H2bc1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	H2bc1
Entrez Gene ID	319177
Full Name	histone cluster 1, H2ba
Synonyms	Th2b
General protein information	Preferred Names histone cluster 1, H2ba Names histone H2B type 1-A histone 1, H2ba histone H2B, testis testis-specific histone H2B
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 13 Map Location: 13\|13 A3.1
Summary	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2A family. Transcripts from this gene contain a palindromic termination element. [provided by RefSeq, Aug 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_175663.2	NP_783594.1	histone H2B type 1-A

Pathways from BioSystems

Homologies

	HIST1H2BA	XP_001086992.1
Rattus norvegicus (Norway rat)	Hist1h2ba	NP_072169.1
Bos taurus (cattle)	HIST1H2BA	XP_005223866.1
Mus musculus (house mouse)	Hist1h2ba	NP_783594.1
Homo sapiens (human)	HIST1H2BA	NP_733759.1
Pan troglodytes (chimpanzee)	HIST1H2BA	XP_527247.1
Canis lupus familiaris (dog)	HIST1H2BA	XP_545374.2
Canis lupus familiaris (dog)	LOC488253	XP_005640164.1

Gene Ontology
Bibliography

Related articles in PubMed

Histone Variant H2A.L.2 Guides Transition Protein-Dependent Protamine Assembly in Male Germ Cells.
Barral S, Morozumi Y, Tanaka H, Montellier E, Govin J, de Dieuleveult M, Charbonnier G, Cout? Y, Puthier D, Buchou T, Boussouar F, Urahama T, Fenaille F, Curtet S, H?ry P, Fernandez-Nunez N, Shiota H, G?rard M, Rousseaux S, Kurumizaka H, Khochbin S
Molecular cell66(1)89-101.e8(2017 Apr)

RNF20 Links Histone H2B Ubiquitylation with Inflammation and Inflammation-Associated Cancer.
Tarcic O, Pateras IS, Cooks T, Shema E, Kanterman J, Ashkenazi H, Boocholez H, Hubert A, Rotkopf R, Baniyash M, Pikarsky E, Gorgoulis VG, Oren M
Cell reports14(6)1462-1476(2016 Feb)

Actin cytoskeleton differentially alters the dynamics of lamin A, HP1? and H2B core histone proteins to remodel chromatin condensation state in living cells.
Toh KC, Ramdas NM, Shivashankar GV
Integrative biology : quantitative biosciences from nano to macro7(10)1309-17(2015 Oct)

Structural and functional analyses of nucleosome complexes with mouse histone variants TH2a and TH2b, involved in reprogramming.
Padavattan S, Shinagawa T, Hasegawa K, Kumasaka T, Ishii S, Kumarevel T
Biochemical and biophysical research communications464(3)929-35(2015 Aug)

Disruption of Th2a and Th2b genes causes defects in spermatogenesis.
Shinagawa T, Huynh LM, Takagi T, Tsukamoto D, Tomaru C, Kwak HG, Dohmae N, Noguchi J, Ishii S
Development (Cambridge, England)142(7)1287-92(2015 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

RNF20 and H2Bub1 promotes chronic colonic inflammation and inflammation-associated colorectal cancer in mice and humans, partly by augmenting NF-kappaB activity and attenuating the antitumoral T cell response.
Title: RNF20 Links Histone H2B Ubiquitylation with Inflammation and Inflammation-Associated Cancer.

demonstrate an actin-driven differential modulation of core histone H2B and heterochromatin HP1alpha protein dynamics with chromatin
Title: Actin cytoskeleton differentially alters the dynamics of lamin A, HP1? and H2B core histone proteins to remodel chromatin condensation state in living cells.

Crystal structure and functional analyses of nucleosome complexes with mouse histone variants TH2a and TH2b, involved in reprogramming.
Title: Structural and functional analyses of nucleosome complexes with mouse histone variants TH2a and TH2b, involved in reprogramming.

These results suggest that a loss of TH2A and TH2B function in chromatin dynamics or a decrease in the total histone levels causes defects in both cohesin release and histone replacement during spermatogenesis.
Title: Disruption of Th2a and Th2b genes causes defects in spermatogenesis.

TH2B is a unique histone variant that plays a key role in the histone-to-protamine packing of the male genome and guides genome-wide chromatin transitions that both precede and follow transmission of the male genome to the egg.
Title: Chromatin-to-nucleoprotamine transition is controlled by the histone H2B variant TH2B.

The following H2bc1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the H2bc1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu16712	NM_175663.2 Latest version!	Mus musculus H2B clustered histone 1 (H2bc1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

H2bc1 ( NM_175663.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_783594.1 Mus musculus H2B clustered histone 1 (H2bc1), mRNA.

Price & Availability↑

CloneID	OMu16712
Clone ID Related Accession (Same CDS sequence)	NM_175663.2
Accession Version	NM_175663.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 384bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-05
Organism	Mus musculus(house mouse)
Product	histone H2B type 1-A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL606464.11. On Feb 21, 2014 this sequence version replaced NM_175663.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## replication-dependent histone :: PMID: 12408966 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCCGGAGG TGGCGGTAAA GGGTGCTACT ATTTCCAAGA AAGGCTTCAA GAAAGCGGTC 
ACCAAGACCC AGAAAAAGGA GGGCCGGAAA CGTAAGAGAT GCCGCAAGGA GAGCTACTCC 
ATTTACATCT ATAAGGTGCT GAAACAAGTG CACCCCGACA CCGGCATCTC CTCCAAGGCC 
ATGAGCATCA TGAACTCCTT TGTGACAGAC ATCTTCGAGC GCATCGCGAG CGAGGCGTCC 
CGCCTGGCGC ATTACAACAA GCGCTCGACC ATCACGTCCC GGGAGATCCA GACGGCCGTG 
CGCCTGCTGC TGCCCGGGGA GCTGGCCAAG CACGCGGTGT CGGAGGGCAC CAAGGCCGTC 
ACCAAGTACA CCAGCTCCAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_783594.1
CDS	1..384
Translation	MPEVAVKGATISKKGFKKAVTKTQKKEGRKRKRCRKESYSIYIYKVLKQVHPDTGISSKAMSIMNSFVTDIFERIASEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK

Target ORF information:

RefSeq Version	NM_175663.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus H2B clustered histone 1 (H2bc1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175663.2

ORF Insert Sequence:

ATGCCGGAGG TGGCGGTAAA GGGTGCTACT ATTTCCAAGA AAGGCTTCAA GAAAGCGGTC 
ACCAAGACCC AGAAAAAGGA GGGCCGGAAA CGTAAGAGAT GCCGCAAGGA GAGCTACTCC 
ATTTACATCT ATAAGGTGCT GAAACAAGTG CACCCCGACA CCGGCATCTC CTCCAAGGCC 
ATGAGCATCA TGAACTCCTT TGTGACAGAC ATCTTCGAGC GCATCGCGAG CGAGGCGTCC 
CGCCTGGCGC ATTACAACAA GCGCTCGACC ATCACGTCCC GGGAGATCCA GACGGCCGTG 
CGCCTGCTGC TGCCCGGGGA GCTGGCCAAG CACGCGGTGT CGGAGGGCAC CAAGGCCGTC 
ACCAAGTACA CCAGCTCCAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Histone Variant H2A.L.2 Guides Transition Protein-Dependent Protamine Assembly in Male Germ Cells.
Molecular cell66(1)89-101.e8(2017 Apr)
Barral S,Morozumi Y,Tanaka H,Montellier E,Govin J,de Dieuleveult M,Charbonnier G,Cout? Y,Puthier D,Buchou T,Boussouar F,Urahama T,Fenaille F,Curtet S,H?ry P,Fernandez-Nunez N,Shiota H,G?rard M,Rousseaux S,Kurumizaka H,Khochbin S

RNF20 Links Histone H2B Ubiquitylation with Inflammation and Inflammation-Associated Cancer.
Cell reports14(6)1462-1476(2016 Feb)
Tarcic O,Pateras IS,Cooks T,Shema E,Kanterman J,Ashkenazi H,Boocholez H,Hubert A,Rotkopf R,Baniyash M,Pikarsky E,Gorgoulis VG,Oren M

Actin cytoskeleton differentially alters the dynamics of lamin A, HP1? and H2B core histone proteins to remodel chromatin condensation state in living cells.
Integrative biology : quantitative biosciences from nano to macro7(10)1309-17(2015 Oct)
Toh KC,Ramdas NM,Shivashankar GV

Structural and functional analyses of nucleosome complexes with mouse histone variants TH2a and TH2b, involved in reprogramming.
Biochemical and biophysical research communications464(3)929-35(2015 Aug)
Padavattan S,Shinagawa T,Hasegawa K,Kumasaka T,Ishii S,Kumarevel T

Disruption of Th2a and Th2b genes causes defects in spermatogenesis.
Development (Cambridge, England)142(7)1287-92(2015 Apr)
Shinagawa T,Huynh LM,Takagi T,Tsukamoto D,Tomaru C,Kwak HG,Dohmae N,Noguchi J,Ishii S

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H2bc1 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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