Aass cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Aass
Entrez Gene ID	30956
Full Name	aminoadipate-semialdehyde synthase
Synonyms	LKR,LKR/SDH,LOR,LOR/SDH,Lorsdh,SDH
General protein information	Preferred Names aminoadipate-semialdehyde synthase Names alpha-aminoadipic semialdehyde synthase, mitochondrial lysine oxoglutarate reductase saccharopine dehydrogenase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6 A3.1\|6 10.27 cM
Summary	This gene encodes a bifunctional mitochondrial protein that catalyzes the first two steps in the lysine degradation pathway. The N-terminus contains lysine-ketoglutarate reductase activity and converts lysine to saccharopine, whereas the C-terminus contains saccharopine dehydrogenase activity and converts saccharopine to alpha-aminoadipate semialdehyde. Mutations in a human gene encoding a highly similar protein are associated with familial hyperlysinemia. [provided by RefSeq, Jul 2008].

mRNA and Protein(s)

mRNA	Protein	Name
NM_013930.4	NP_038958.2	alpha-aminoadipic semialdehyde synthase, mitochondrial

Pathways from BioSystems

Homologies

	AASS	XP_002803495.1
Canis lupus familiaris (dog)	AASS	XP_005628825.1
Bos taurus (cattle)	AASS	NP_001103267.1
Rattus norvegicus (Norway rat)	Aass	NP_001094433.1
Magnaporthe oryzae (rice blast fungus)	MGG_17041	XP_003716085.1
Arabidopsis thaliana (thale cress)	AT4G33150	NP_567914.1
Homo sapiens (human)	AASS	NP_005754.2
Gallus gallus (chicken)	AASS	XP_416001.2
Schizosaccharomyces pombe (fission yeast)	SPBC3B8.03	NP_596411.1
Oryza sativa (rice)	Os02g0783700	NP_001048324.1
Pan troglodytes (chimpanzee)	AASS	XP_001145257.1
Caenorhabditis elegans (roundworm)	CELE_R02D3.1	NP_499884.1
Neurospora crassa	NCU03748	XP_961095.2
Xenopus tropicalis (tropical clawed frog)	aass	NP_001011437.1
Mus musculus (house mouse)	Aass	NP_038958.2
Danio rerio (zebrafish)	aass	NP_001167456.1
Drosophila melanogaster (fruit fly)	LKR	NP_609150.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008632	XP_314728.3
Saccharomyces cerevisiae (baker's yeast)	LYS9	NP_014448.1
Kluyveromyces lactis	KLLA0C18744g	XP_453035.1

Gene Ontology
Bibliography

Related articles in PubMed

Global gene expression analysis of murine limb development.
Taher L, Collette NM, Murugesh D, Maxwell E, Ovcharenko I, Loots GG
PloS one6(12)e28358(2011)

Protein-induced alterations in murine hepatic alpha-aminoadipate delta-semialdehyde synthase activity are mediated posttranslationally.
Kiess AS, Cleveland BM, Wilson ME, Klandorf H, Blemings KP
Nutrition research (New York, N.Y.)28(12)859-65(2008 Dec)

Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria.
Mootha VK, Bunkenborg J, Olsen JV, Hjerrild M, Wisniewski JR, Stahl E, Bolouri MS, Ray HN, Sihag S, Kamal M, Patterson N, Lander ES, Mann M
Cell115(5)629-40(2003 Nov)

The essential amino acid lysine acts as precursor of glutamate in the mammalian central nervous system.
Papes F, Surpili MJ, Langone F, Trigo JR, Arruda P
FEBS letters488(1-2)34-8(2001 Jan)

Lysine degradation through the saccharopine pathway in mammals: involvement of both bifunctional and monofunctional lysine-degrading enzymes in mouse.
Papes F, Kemper EL, Cord-Neto G, Langone F, Arruda P
The Biochemical journal344 Pt 2555-63(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mice receiving the high-protein diet had greater AASS mRNA compared to mice fed the adequate protein diet.
Title: Protein-induced alterations in murine hepatic alpha-aminoadipate delta-semialdehyde synthase activity are mediated posttranslationally.

These results indicate AASS knockdown decreases the lysine requirement of the cell via a reduction of lysine catabolism through the saccharopine pathway.
Title: Alpha-aminoadipate delta-semialdehyde synthase mRNA knockdown reduces the lysine requirement of a mouse hepatic cell line.

The following Aass gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Aass cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu16036	NM_013930.4 Latest version!	Mus musculus aminoadipate-semialdehyde synthase (Aass), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Aass ( NM_013930.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_038958.2 Mus musculus aminoadipate-semialdehyde synthase (Aass), mRNA.

Price & Availability↑

CloneID	OMu16036
Clone ID Related Accession (Same CDS sequence)	NM_013930.4
Accession Version	NM_013930.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2781bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	alpha-aminoadipic semialdehyde synthase, mitochondrial
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CJ074697.1, AK149400.1 and AC133599.3. On Mar 5, 2010 this sequence version replaced NM_013930.3. ##Evidence-Data-START## Transcript exon combination :: AK149400.1, AK044323.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164135 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGCTGAGAG CGCAGAGGCC GAGACTGGCC AGGCTTAGAG CCTGCCTCTC CAGAGGCCTC 
CACCACAAGC CAGTGATGGC ACTCCGGAGG GAGGACGTGA ACGCCTGGGA GCGCAGGGCG 
CCTCTGGCTC CCAAGCACAT CAAGGGCATC ACCAAACTGG GCTACAAGGT CTTGATACAG 
CCTTCCAACA GACGGGCCAT CCATGACAAG GAGTATGTCA GGGCTGGTGG CATTCTTCAG 
GAAGACATCA CAGAAGCCTG TCTGATTTTG GGAGTAAAAA GGCCTCCAGA GGAAAAACTG 
ATGTCCAAGA AGACGTACGC CTTCTTTTCC CACACAATAA AAGCTCAGGA AGCCAATATG 
AACCTGTTGG ATGAGGTTCT GAAACAGGAA ATCCGGCTCA TTGATTATGA GAAAATGGTG 
GATCACAGAG GGTCTCGGAT AGTGGCTTTC GGACAGTGGG CGGGTGTGGC AGGAATGATT 
AATATTTTAC ATGGAATGGG CTTAAGGCTC CTTGCTTTGG GACATCATAC GCCTTTTATG 
CATCTTGGCA TGGCTCACAA CTACAGGAAC AGCAGCCAGG CCGTGCAGGC TGTCCGTGAT 
GCTGGCTATG AAATATCTCT GGGTTTAATG CCAAAGTCAA TAGGACCCTT AACGTTTGTC 
TTCACAGGGA CTGGCAATGT ATCCAAGGGA GCCCAGGAAG TCTTTAATGA GCTACCTTGT 
GAGTATGTGG AGCCCCACGA ATTAAGAGAA GTTTCAAAAA CTGGAGACCT CAGGAAAGTG 
TATGGGACGG TGTTAAGTCG CCATCATCAT CTTGTCAGGA AAACAGATGG TGTGTACGAT 
CCCGTAGAAT ACGAGAAATA TCCTGAGCGC TACACAAGTC GTTTTAATAC AGATATTGCG 
CCCTATACAA CTTGTTTAAT TAATGGAATC TACTGGGAGC AAAACACCCC TCGCCTGCTC 
ACACGTCAGG ATGCTCAGAG TCTCCTGGTC CCAGTGAAGT CCTCAGTTGT TCCTGTTGAA 
GGATGTCCTG AATTACCACA CAAACTTGTG GCAATATGTG ACATTTCAGC AGACACGGGA 
GGGTCCATAG ATTTTATGAC TGAGTGCACC ACCATAGAAC GGCCCTTTTG CATGTATGAC 
GCAGACCAGC AAATTATTCA CGACAGTGTT GAAGGCTCCG GGATCCTGAT GTGTTCCATT 
GACAATTTGC CAGCACAGCT TCCAATCGAG GCTACGGAAT ACTTTGGAGA CATGCTTTAC 
CCTTATGTTG AAGAAATGTT GTTATCCGAT GCATCACAAC CTCTGGAAAG CCAGAACTTC 
TCCCCAGTGG TGCGAGACGC AGTGATTACG TCCAATGGTC TATTGACTGA CAAATACAAA 
TATATACAGA AACTCCGAGA AAGCAGAGAA CGCATTCAAT TCCTTTCAAT GAGTACCAAG 
AAGAAGGTTT TGGTCCTTGG GTCTGGCTAT GTATCTGGGC CTGTTTTGGA ATATCTGTCA 
AGAGACAACA ATATAGAAAT AACACTAGGC TCTGACATGA CCAACCAAAT GCAGCAGCTG 
AGCAAGAAAT ACAACATTAA CCCTGTCAGT TTGACTGTTG GCAAGCAAGA GGCAAAGCTG 
CAATCCCTGG TGGAATCCCA GGATCTTGTG ATCAGCTTGT TGCCTTATGT GCTTCATCCT 
GTGGTGGCCA AAGCCTGCAT CGAAAGCAGG GTTAATATGG TCACTGCAAG CTATATTACA 
CCAGCCATGA AAGAACTGGA AAAGAGTGTG GATGATGCTG GCATTACAGT CATTGGTGAG 
TTGGGATTAG ACCCTGGTCT TGATCACATG TTGGCAATGG AAACAATTGA TACTGCCAAA 
GAGCTGGGAG CCACGGTTGA GTCTTATGTT TCCTACTGTG GTGGCCTTCC GGCCCCTGAA 
CATTCAGATA ATCCCTTGCG ATACAAATTC AGCTGGAGTC CAGTGGGAGT CTTGATGAAC 
ATAATGCAGC CTGCTAGCTA TCTGCTCAAT GGGAAGGTTG TGAATGTGAC AGGGGGAGTC 
TCTTTTCTCA ACTCTGTCAC ACCCATGGAT TACTTCCCAG GTCTAAACTT GGAAGGCTAT 
CCTAACAGAG ATAGCATAAA ATATGCTGAG ATTTATGGAA TTTCATCTGC CCATACATTA 
TTAAGGGGAA CACTGAGATA TAAGGGATAT TCCAAAGCCT TGAATGGATT TGTGAAGTTG 
GGACTCATCA ACAGAGAAGC ATACCCTGCC CTCCGACCTG AGGCCAACCC TCTCACCTGG 
AAACAACTCC TATGTGACCT AGTTGGAATT TCCCGCTCCT CCCCATGCGA GAAGCTTAAG 
GAAGTTGTCT TCACAAAGCT GGGAGGGGAC AATACCCAGC TGGAAGCTGC AGAATGGTTG 
GGCTTACTGG GGGATGAACA AGTTCCTCAG GCGGAGTCCA TTGTGGACGC GTTCTCCAAG 
CACTTGGTCT CAAAACTCTC CTATGGCCCT GAAGAAAAGG ATATGATTGT GATGAGGGAC 
AGCTTTGGCA TCAGACATCC ATCTGGACAT TTAGAAAATA AGACCATTGA TCTCGTGGTT 
TATGGAGACT TCAACGGCTT CTCGGCAATG GCTAAAACTG TGGGGTTGCC GACAGCCATG 
GCAGCCAAAA TGTTGCTTGA TGGTGAAATT GAAGCCAAAG GCCTAATGGG GCCCTTTACA 
AAGGAGATCT ATGGGCCAAT ATTGGAGCGG ATTAAAGCAG AAGGCATTGT ATTTAACACA 
CAGAGTACAA TCAAGCTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038958.2
CDS	122..2902
Translation	MLRAQRPRLARLRACLSRGLHHKPVMALRREDVNAWERRAPLAPKHIKGITKLGYKVLIQPSNRRAIHDKEYVRAGGILQEDITEACLILGVKRPPEEKLMSKKTYAFFSHTIKAQEANMNLLDEVLKQEIRLIDYEKMVDHRGSRIVAFGQWAGVAGMINILHGMGLRLLALGHHTPFMHLGMAHNYRNSSQAVQAVRDAGYEISLGLMPKSIGPLTFVFTGTGNVSKGAQEVFNELPCEYVEPHELREVSKTGDLRKVYGTVLSRHHHLVRKTDGVYDPVEYEKYPERYTSRFNTDIAPYTTCLINGIYWEQNTPRLLTRQDAQSLLVPVKSSVVPVEGCPELPHKLVAICDISADTGGSIDFMTECTTIERPFCMYDADQQIIHDSVEGSGILMCSIDNLPAQLPIEATEYFGDMLYPYVEEMLLSDASQPLESQNFSPVVRDAVITSNGLLTDKYKYIQKLRESRERIQFLSMSTKKKVLVLGSGYVSGPVLEYLSRDNNIEITLGSDMTNQMQQLSKKYNINPVSLTVGKQEAKLQSLVESQDLVISLLPYVLHPVVAKACIESRVNMVTASYITPAMKELEKSVDDAGITVIGELGLDPGLDHMLAMETIDTAKELGATVESYVSYCGGLPAPEHSDNPLRYKFSWSPVGVLMNIMQPASYLLNGKVVNVTGGVSFLNSVTPMDYFPGLNLEGYPNRDSIKYAEIYGISSAHTLLRGTLRYKGYSKALNGFVKLGLINREAYPALRPEANPLTWKQLLCDLVGISRSSPCEKLKEVVFTKLGGDNTQLEAAEWLGLLGDEQVPQAESIVDAFSKHLVSKLSYGPEEKDMIVMRDSFGIRHPSGHLENKTIDLVVYGDFNGFSAMAKTVGLPTAMAAKMLLDGEIEAKGLMGPFTKEIYGPILERIKAEGIVFNTQSTIKL

Target ORF information:

RefSeq Version	NM_013930.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus aminoadipate-semialdehyde synthase (Aass), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013930.4

ORF Insert Sequence:

ATGCTGAGAG CGCAGAGGCC GAGACTGGCC AGGCTTAGAG CCTGCCTCTC CAGAGGCCTC 
CACCACAAGC CAGTGATGGC ACTCCGGAGG GAGGACGTGA ACGCCTGGGA GCGCAGGGCG 
CCTCTGGCTC CCAAGCACAT CAAGGGCATC ACCAAACTGG GCTACAAGGT CTTGATACAG 
CCTTCCAACA GACGGGCCAT CCATGACAAG GAGTATGTCA GGGCTGGTGG CATTCTTCAG 
GAAGACATCA CAGAAGCCTG TCTGATTTTG GGAGTAAAAA GGCCTCCAGA GGAAAAACTG 
ATGTCCAAGA AGACGTACGC CTTCTTTTCC CACACAATAA AAGCTCAGGA AGCCAATATG 
AACCTGTTGG ATGAGGTTCT GAAACAGGAA ATCCGGCTCA TTGATTATGA GAAAATGGTG 
GATCACAGAG GGTCTCGGAT AGTGGCTTTC GGACAGTGGG CGGGTGTGGC AGGAATGATT 
AATATTTTAC ATGGAATGGG CTTAAGGCTC CTTGCTTTGG GACATCATAC GCCTTTTATG 
CATCTTGGCA TGGCTCACAA CTACAGGAAC AGCAGCCAGG CCGTGCAGGC TGTCCGTGAT 
GCTGGCTATG AAATATCTCT GGGTTTAATG CCAAAGTCAA TAGGACCCTT AACGTTTGTC 
TTCACAGGGA CTGGCAATGT ATCCAAGGGA GCCCAGGAAG TCTTTAATGA GCTACCTTGT 
GAGTATGTGG AGCCCCACGA ATTAAGAGAA GTTTCAAAAA CTGGAGACCT CAGGAAAGTG 
TATGGGACGG TGTTAAGTCG CCATCATCAT CTTGTCAGGA AAACAGATGG TGTGTACGAT 
CCCGTAGAAT ACGAGAAATA TCCTGAGCGC TACACAAGTC GTTTTAATAC AGATATTGCG 
CCCTATACAA CTTGTTTAAT TAATGGAATC TACTGGGAGC AAAACACCCC TCGCCTGCTC 
ACACGTCAGG ATGCTCAGAG TCTCCTGGTC CCAGTGAAGT CCTCAGTTGT TCCTGTTGAA 
GGATGTCCTG AATTACCACA CAAACTTGTG GCAATATGTG ACATTTCAGC AGACACGGGA 
GGGTCCATAG ATTTTATGAC TGAGTGCACC ACCATAGAAC GGCCCTTTTG CATGTATGAC 
GCAGACCAGC AAATTATTCA CGACAGTGTT GAAGGCTCCG GGATCCTGAT GTGTTCCATT 
GACAATTTGC CAGCACAGCT TCCAATCGAG GCTACGGAAT ACTTTGGAGA CATGCTTTAC 
CCTTATGTTG AAGAAATGTT GTTATCCGAT GCATCACAAC CTCTGGAAAG CCAGAACTTC 
TCCCCAGTGG TGCGAGACGC AGTGATTACG TCCAATGGTC TATTGACTGA CAAATACAAA 
TATATACAGA AACTCCGAGA AAGCAGAGAA CGCATTCAAT TCCTTTCAAT GAGTACCAAG 
AAGAAGGTTT TGGTCCTTGG GTCTGGCTAT GTATCTGGGC CTGTTTTGGA ATATCTGTCA 
AGAGACAACA ATATAGAAAT AACACTAGGC TCTGACATGA CCAACCAAAT GCAGCAGCTG 
AGCAAGAAAT ACAACATTAA CCCTGTCAGT TTGACTGTTG GCAAGCAAGA GGCAAAGCTG 
CAATCCCTGG TGGAATCCCA GGATCTTGTG ATCAGCTTGT TGCCTTATGT GCTTCATCCT 
GTGGTGGCCA AAGCCTGCAT CGAAAGCAGG GTTAATATGG TCACTGCAAG CTATATTACA 
CCAGCCATGA AAGAACTGGA AAAGAGTGTG GATGATGCTG GCATTACAGT CATTGGTGAG 
TTGGGATTAG ACCCTGGTCT TGATCACATG TTGGCAATGG AAACAATTGA TACTGCCAAA 
GAGCTGGGAG CCACGGTTGA GTCTTATGTT TCCTACTGTG GTGGCCTTCC GGCCCCTGAA 
CATTCAGATA ATCCCTTGCG ATACAAATTC AGCTGGAGTC CAGTGGGAGT CTTGATGAAC 
ATAATGCAGC CTGCTAGCTA TCTGCTCAAT GGGAAGGTTG TGAATGTGAC AGGGGGAGTC 
TCTTTTCTCA ACTCTGTCAC ACCCATGGAT TACTTCCCAG GTCTAAACTT GGAAGGCTAT 
CCTAACAGAG ATAGCATAAA ATATGCTGAG ATTTATGGAA TTTCATCTGC CCATACATTA 
TTAAGGGGAA CACTGAGATA TAAGGGATAT TCCAAAGCCT TGAATGGATT TGTGAAGTTG 
GGACTCATCA ACAGAGAAGC ATACCCTGCC CTCCGACCTG AGGCCAACCC TCTCACCTGG 
AAACAACTCC TATGTGACCT AGTTGGAATT TCCCGCTCCT CCCCATGCGA GAAGCTTAAG 
GAAGTTGTCT TCACAAAGCT GGGAGGGGAC AATACCCAGC TGGAAGCTGC AGAATGGTTG 
GGCTTACTGG GGGATGAACA AGTTCCTCAG GCGGAGTCCA TTGTGGACGC GTTCTCCAAG 
CACTTGGTCT CAAAACTCTC CTATGGCCCT GAAGAAAAGG ATATGATTGT GATGAGGGAC 
AGCTTTGGCA TCAGACATCC ATCTGGACAT TTAGAAAATA AGACCATTGA TCTCGTGGTT 
TATGGAGACT TCAACGGCTT CTCGGCAATG GCTAAAACTG TGGGGTTGCC GACAGCCATG 
GCAGCCAAAA TGTTGCTTGA TGGTGAAATT GAAGCCAAAG GCCTAATGGG GCCCTTTACA 
AAGGAGATCT ATGGGCCAAT ATTGGAGCGG ATTAAAGCAG AAGGCATTGT ATTTAACACA 
CAGAGTACAA TCAAGCTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Global gene expression analysis of murine limb development.
PloS one6(12)e28358(2011)
Taher L,Collette NM,Murugesh D,Maxwell E,Ovcharenko I,Loots GG

Protein-induced alterations in murine hepatic alpha-aminoadipate delta-semialdehyde synthase activity are mediated posttranslationally.
Nutrition research (New York, N.Y.)28(12)859-65(2008 Dec)
Kiess AS,Cleveland BM,Wilson ME,Klandorf H,Blemings KP

Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria.
Cell115(5)629-40(2003 Nov)
Mootha VK,Bunkenborg J,Olsen JV,Hjerrild M,Wisniewski JR,Stahl E,Bolouri MS,Ray HN,Sihag S,Kamal M,Patterson N,Lander ES,Mann M

The essential amino acid lysine acts as precursor of glutamate in the mammalian central nervous system.
FEBS letters488(1-2)34-8(2001 Jan)
Papes F,Surpili MJ,Langone F,Trigo JR,Arruda P

Lysine degradation through the saccharopine pathway in mammals: involvement of both bifunctional and monofunctional lysine-degrading enzymes in mouse.
The Biochemical journal344 Pt 2555-63(1999 Dec)
Papes F,Kemper EL,Cord-Neto G,Langone F,Arruda P

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Aass cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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