Usp21 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Usp21
Entrez Gene ID	30941
Full Name	ubiquitin specific peptidase 21
Synonyms	ESTM28,Usp16,Usp23,W53272
General protein information	Preferred Names ubiquitin specific peptidase 21 Names ubiquitin carboxyl-terminal hydrolase 21 deubiquitinating enzyme 21 ubiquitin specific protease 21 ubiquitin specific protease 23 ubiquitin thioesterase 21 ubiquitin thiolesterase 21 ubiquitin-specific-processing protease 21
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1 H3\|1 79.3 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_013919.4	NP_038947.2	ubiquitin carboxyl-terminal hydrolase 21

Pathways from BioSystems

Homologies

	USP21	XP_001117779.2
Bos taurus (cattle)	USP21	NP_001039841.1
Mus musculus (house mouse)	Usp21	NP_038947.2
Rattus norvegicus (Norway rat)	Usp21	NP_001121110.1
Danio rerio (zebrafish)	LOC563546	XP_692003.2
Xenopus tropicalis (tropical clawed frog)	usp21	XP_002942482.2
Canis lupus familiaris (dog)	USP21	XP_536136.3
Homo sapiens (human)	USP21	NP_036607.3
Pan troglodytes (chimpanzee)	USP21	XP_524939.4

Gene Ontology
Bibliography

Related articles in PubMed

p38 inhibition provides anti-DNA virus immunity by regulation of USP21 phosphorylation and STING activation.
Chen Y, Wang L, Jin J, Luan Y, Chen C, Li Y, Chu H, Wang X, Liao G, Yu Y, Teng H, Wang Y, Pan W, Fang L, Liao L, Jiang Z, Ge X, Li B, Wang P
The Journal of experimental medicine214(4)991-1010(2017 Apr)

USP21 prevents the generation of T-helper-1-like Treg cells.
Li Y, Lu Y, Wang S, Han Z, Zhu F, Ni Y, Liang R, Zhang Y, Leng Q, Wei G, Shi G, Zhu R, Li D, Wang H, Zheng SG, Xu H, Tsun A, Li B
Nature communications713559(2016 Nov)

The centrosomal deubiquitylase USP21 regulates Gli1 transcriptional activity and stability.
Heride C, Rigden DJ, Bertsoulaki E, Cucchi D, De Smaele E, Clague MJ, Urb? S
Journal of cell science129(21)4001-4013(2016 11)

The deubiquitinase USP21 maintains the stemness of mouse embryonic stem cells via stabilization of Nanog.
Jin J, Liu J, Chen C, Liu Z, Jiang C, Chu H, Pan W, Wang X, Zhang L, Li B, Jiang C, Ge X, Xie X, Wang P
Nature communications713594(2016 Nov)

Ubiquitin specific protease 21 is dispensable for normal development, hematopoiesis and lymphocyte differentiation.
Pannu J, Belle JI, F?rster M, Duerr CU, Shen S, Kane L, Harcourt K, Fritz JH, Clare S, Nijnik A
PloS one10(2)e0117304(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

USP21 recruits and stabilises Gli1 at the centrosome.
Title: The centrosomal deubiquitylase USP21 regulates Gli1 transcriptional activity and stability.

Ubiquitin specific protease 21 is dispensable for normal development, hematopoiesis and lymphocyte differentiation
Title: Ubiquitin specific protease 21 is dispensable for normal development, hematopoiesis and lymphocyte differentiation.

Overexpression of USP21 inhibited RNA virus-induced RIG-I polyubiquitination and RIG-I-mediated interferon signaling, while USP21 deletion resulted in elevated RIG-I polyubiquitination, IRF3 phosphorylation, IFN production, and antiviral responses.
Title: USP21 negatively regulates antiviral response by acting as a RIG-I deubiquitinase.

USP21 plays an important role in the down-regulation of TNFalpha-induced NF-kappaB activation through deubiquitinating RIP1.
Title: Ubiquitin-specific peptidase 21 inhibits tumor necrosis factor alpha-induced nuclear factor kappaB activation via binding to and deubiquitinating receptor-interacting protein 1.

The following Usp21 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Usp21 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu18092	NM_013919.4 Latest version!	Mus musculus ubiquitin specific peptidase 21 (Usp21), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Usp21 ( NM_013919.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_038947.2 Mus musculus ubiquitin specific peptidase 21 (Usp21), mRNA.

Price & Availability↑

CloneID	OMu18092
Clone ID Related Accession (Same CDS sequence)	NM_013919.4
Accession Version	NM_013919.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1701bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Mus musculus(house mouse)
Product	ubiquitin carboxyl-terminal hydrolase 21
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY110597.1, AK011148.1, AF177759.1 and BP770216.1. On Mar 11, 2009 this sequence version replaced NM_013919.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660815.169981.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164138, SAMN01164143 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCCAGG CTTCTGAGCA CCGCCTGGGC CGGACTCGAG AGCCACCTGT CAATGTACAG 
CCCCGAGTGG GAGCCAAGAT ACCATTTCCT CCCCGGGCAC GCAGCAAGGA GCGCCGAAAC 
CCAGTTCCTG GGCCAAACTC CATGTTACGA CCTTTGCCTC CCAGGCCAGG TCCCCCAGAT 
GAAAGGCTCA AGAAACTGGA GCTGGGTCGG GGTCGGACCT CAGGCTCTCG TCCTAGAGGT 
CCCCTTCGAG CAGATCATGG GGTTCCCCTG CCTGGCTCAC CGCCCCCAGC TGTGGCTCTG 
CCTCTCCCAT CCCGGACCAA CTTAGCCCGT TCCAAGTCTG TGAGCAGTGG GGACTTGCGT 
CCAATGGGGA TTGCCTTGGG AGGGCACCGT GGCGCTGGCG AGCTAGGGGC TGCACTGAGC 
CGCTTGGCAC TCCGGCCTGA GCCACCCACT TTGAGACGTA GTACTTCTCT CCGGCGTCTT 
GGGGGCTTCC CTGGCCCCCC TACCCTGCTC AGCATACGGA CAGAGCCCCC TACTTCCCAT 
GGCTCCTTCC ACATGATATC TGCCCGGCCT TCTGAGCCTT TCTACTCTGA TGACAAGATG 
GCTCACCACA CACTGCTTCT GGGCTCTGGT CATGTTGGCC TCCGAAATCT AGGAAATACA 
TGCTTCCTGA ATGCCGTGCT ACAGTGTTTG AGCAGCACAA GGCCTCTTCG AGACTTCTGT 
CTGCGAAGGG ACTTCCGGCA AGAGGTGCCC GGAGGAGGCC GAGCCCAGGA ACTCACAGAA 
GCCTTTGCAG ATGTGATTGG TGCCCTCTGG CACCCTGACT CCTGTGAAGC TGTGAATCCC 
ACCCGATTCC GGGCTGTCTT CCAGAAATAC GTCCCTTCCT TCTCTGGATA CAGCCAGCAG 
GATGCCCAAG AGTTCCTGAA GCTCCTCATG GAGCGGTTGC ACCTTGAGAT CAACCGACGA 
GGGCGCCGGG CACCGCCAAT CTTGGCCAGC GGACCAGTTC CCTCTCCACC TTGCCGAGGA 
GGAGGGGCTC TGCATGAAGA ACCTGAGTTG AGTGATGATG ACCGAGCCAA CTTAATGTGG 
AAGCGCTACC TGGAGCGAGA AGACAGCAAG ATTGTGGACC TGTTTGTGGG CCAGTTGAAA 
AGTTGCCTCA AGTGCCAGGC CTGTGGGTAT CGCTCCACGA CCTTCGAGGT TTTTTGTGAC 
CTGTCCCTGC CCATCCCCAA GAAAGGATTT GCTGGGGGCA AAGTGTCTTT GCGGGATTGC 
TTCAGCCTTT TCACCAAGGA GGAAGAGCTA GAGTCGGAGA ATGCCCCAGT GTGTGACCGA 
TGTCGGCAGA AAACCCGAAG TACCAAAAAG TTGACAGTAC AAAGATTCCC CCGAATCCTC 
GTGCTCCATC TGAACCGATT TTCCACCTCC CGAGGCTCCA TCAAGAAAAG TTCAGTAGGT 
GTAGACTTCC CACTGCAGAG ACTAAGCCTA GGGGACTTTG CCAGCGACAA AGCGGGAAGC 
CCTGTCTACC AGCTGTATGC CCTTTGCAAC CACTCGGGTA GTGTCCACTA TGGCCACTAC 
ACAGCCCTGT GCCGGTGCCA GACTGGTTGG CATGTCTACA ATGACTCCCG CGTTTCCCCT 
GTCAGTGAAA ATCAGGTGGC ATCCAGTGAG GGCTACGTGC TGTTCTACCA ATTGATGCAG 
GAACCACTTC GGTGCCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038947.2
CDS	222..1922
Translation	MPQASEHRLGRTREPPVNVQPRVGAKIPFPPRARSKERRNPVPGPNSMLRPLPPRPGPPDERLKKLELGRGRTSGSRPRGPLRADHGVPLPGSPPPAVALPLPSRTNLARSKSVSSGDLRPMGIALGGHRGAGELGAALSRLALRPEPPTLRRSTSLRRLGGFPGPPTLLSIRTEPPTSHGSFHMISARPSEPFYSDDKMAHHTLLLGSGHVGLRNLGNTCFLNAVLQCLSSTRPLRDFCLRRDFRQEVPGGGRAQELTEAFADVIGALWHPDSCEAVNPTRFRAVFQKYVPSFSGYSQQDAQEFLKLLMERLHLEINRRGRRAPPILASGPVPSPPCRGGGALHEEPELSDDDRANLMWKRYLEREDSKIVDLFVGQLKSCLKCQACGYRSTTFEVFCDLSLPIPKKGFAGGKVSLRDCFSLFTKEEELESENAPVCDRCRQKTRSTKKLTVQRFPRILVLHLNRFSTSRGSIKKSSVGVDFPLQRLSLGDFASDKAGSPVYQLYALCNHSGSVHYGHYTALCRCQTGWHVYNDSRVSPVSENQVASSEGYVLFYQLMQEPLRCL

Target ORF information:

RefSeq Version	NM_013919.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin specific peptidase 21 (Usp21), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013919.4

ORF Insert Sequence:

ATGCCCCAGG CTTCTGAGCA CCGCCTGGGC CGGACTCGAG AGCCACCTGT CAATGTACAG 
CCCCGAGTGG GAGCCAAGAT ACCATTTCCT CCCCGGGCAC GCAGCAAGGA GCGCCGAAAC 
CCAGTTCCTG GGCCAAACTC CATGTTACGA CCTTTGCCTC CCAGGCCAGG TCCCCCAGAT 
GAAAGGCTCA AGAAACTGGA GCTGGGTCGG GGTCGGACCT CAGGCTCTCG TCCTAGAGGT 
CCCCTTCGAG CAGATCATGG GGTTCCCCTG CCTGGCTCAC CGCCCCCAGC TGTGGCTCTG 
CCTCTCCCAT CCCGGACCAA CTTAGCCCGT TCCAAGTCTG TGAGCAGTGG GGACTTGCGT 
CCAATGGGGA TTGCCTTGGG AGGGCACCGT GGCGCTGGCG AGCTAGGGGC TGCACTGAGC 
CGCTTGGCAC TCCGGCCTGA GCCACCCACT TTGAGACGTA GTACTTCTCT CCGGCGTCTT 
GGGGGCTTCC CTGGCCCCCC TACCCTGCTC AGCATACGGA CAGAGCCCCC TACTTCCCAT 
GGCTCCTTCC ACATGATATC TGCCCGGCCT TCTGAGCCTT TCTACTCTGA TGACAAGATG 
GCTCACCACA CACTGCTTCT GGGCTCTGGT CATGTTGGCC TCCGAAATCT AGGAAATACA 
TGCTTCCTGA ATGCCGTGCT ACAGTGTTTG AGCAGCACAA GGCCTCTTCG AGACTTCTGT 
CTGCGAAGGG ACTTCCGGCA AGAGGTGCCC GGAGGAGGCC GAGCCCAGGA ACTCACAGAA 
GCCTTTGCAG ATGTGATTGG TGCCCTCTGG CACCCTGACT CCTGTGAAGC TGTGAATCCC 
ACCCGATTCC GGGCTGTCTT CCAGAAATAC GTCCCTTCCT TCTCTGGATA CAGCCAGCAG 
GATGCCCAAG AGTTCCTGAA GCTCCTCATG GAGCGGTTGC ACCTTGAGAT CAACCGACGA 
GGGCGCCGGG CACCGCCAAT CTTGGCCAGC GGACCAGTTC CCTCTCCACC TTGCCGAGGA 
GGAGGGGCTC TGCATGAAGA ACCTGAGTTG AGTGATGATG ACCGAGCCAA CTTAATGTGG 
AAGCGCTACC TGGAGCGAGA AGACAGCAAG ATTGTGGACC TGTTTGTGGG CCAGTTGAAA 
AGTTGCCTCA AGTGCCAGGC CTGTGGGTAT CGCTCCACGA CCTTCGAGGT TTTTTGTGAC 
CTGTCCCTGC CCATCCCCAA GAAAGGATTT GCTGGGGGCA AAGTGTCTTT GCGGGATTGC 
TTCAGCCTTT TCACCAAGGA GGAAGAGCTA GAGTCGGAGA ATGCCCCAGT GTGTGACCGA 
TGTCGGCAGA AAACCCGAAG TACCAAAAAG TTGACAGTAC AAAGATTCCC CCGAATCCTC 
GTGCTCCATC TGAACCGATT TTCCACCTCC CGAGGCTCCA TCAAGAAAAG TTCAGTAGGT 
GTAGACTTCC CACTGCAGAG ACTAAGCCTA GGGGACTTTG CCAGCGACAA AGCGGGAAGC 
CCTGTCTACC AGCTGTATGC CCTTTGCAAC CACTCGGGTA GTGTCCACTA TGGCCACTAC 
ACAGCCCTGT GCCGGTGCCA GACTGGTTGG CATGTCTACA ATGACTCCCG CGTTTCCCCT 
GTCAGTGAAA ATCAGGTGGC ATCCAGTGAG GGCTACGTGC TGTTCTACCA ATTGATGCAG 
GAACCACTTC GGTGCCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

p38 inhibition provides anti-DNA virus immunity by regulation of USP21 phosphorylation and STING activation.
The Journal of experimental medicine214(4)991-1010(2017 Apr)
Chen Y,Wang L,Jin J,Luan Y,Chen C,Li Y,Chu H,Wang X,Liao G,Yu Y,Teng H,Wang Y,Pan W,Fang L,Liao L,Jiang Z,Ge X,Li B,Wang P

USP21 prevents the generation of T-helper-1-like Treg cells.
Nature communications713559(2016 Nov)
Li Y,Lu Y,Wang S,Han Z,Zhu F,Ni Y,Liang R,Zhang Y,Leng Q,Wei G,Shi G,Zhu R,Li D,Wang H,Zheng SG,Xu H,Tsun A,Li B

The centrosomal deubiquitylase USP21 regulates Gli1 transcriptional activity and stability.
Journal of cell science129(21)4001-4013(2016 11)
Heride C,Rigden DJ,Bertsoulaki E,Cucchi D,De Smaele E,Clague MJ,Urb? S

The deubiquitinase USP21 maintains the stemness of mouse embryonic stem cells via stabilization of Nanog.
Nature communications713594(2016 Nov)
Jin J,Liu J,Chen C,Liu Z,Jiang C,Chu H,Pan W,Wang X,Zhang L,Li B,Jiang C,Ge X,Xie X,Wang P

Ubiquitin specific protease 21 is dispensable for normal development, hematopoiesis and lymphocyte differentiation.
PloS one10(2)e0117304(2015)
Pannu J,Belle JI,F?rster M,Duerr CU,Shen S,Kane L,Harcourt K,Fritz JH,Clare S,Nijnik A

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Usp21 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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