APLN cDNA ORF clone, Mus musculus(House mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	APLN
Entrez Gene ID	30878
Full Name	apelin
Synonyms	6030430G11Rik,Apel
General protein information	Preferred Names apelin Names apelin APJ endogenous ligand preproapelin
Gene Type	protein-coding
Organism	Mus musculus(House mouse)
Genome	Chromosome: X Map Location: X\|X A5
Summary	This gene encodes the neuropeptide Apelin, an endogenous ligand of the G protein-coupled receptor APJ. This gene is highly expressed in the central nervous system and peripheral tissues and the encoded preproprotein undergoes proteolytic processing to generate mature peptides of different sizes that are secreted into the plasma. These peptides play important roles in a broad range of physiological processes such as cardiac contractility, blood pressure, blood vessel growth, appetite and drink behavior, and pituitary hormone secretion. Mice lacking the encoded protein develop progressive heart failure and exhibit a shorter bleeding time, prothrombotic profile and increased bone mass. [provided by RefSeq, Jul 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_013912.4	NP_038940.1	apelin preproprotein
NM_013912.3	NP_038940.1	apelin precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Apln	NP_113800.1
Homo sapiens (human)	APLN	NP_059109.3
Bos taurus (cattle)	APLN	NP_776928.1
Canis lupus familiaris (dog)	APLN	XP_854262.2
Mus musculus (house mouse)	Apln	NP_038940.1

Gene Ontology

The following APLN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the APLN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu19425	NM_013912.4 Latest version!	Mus musculus apelin (Apln), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
View Old Accession Versions >>
OMu19425	NM_013912.3	Mus musculus apelin (Apln), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

APLN ( NM_013912.4 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_038940.1 Mus musculus apelin (Apln), mRNA.

Price & Availability↑

CloneID	OMu19425
Clone ID Related Accession (Same CDS sequence)	NM_013912.4 , NM_013912.3
Accession Version	NM_013912.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 234bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Mus musculus(house mouse)
Product	apelin preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL672274.7 and DQ832282.1. On Jul 15, 2016 this sequence version replaced NM_013912.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ832282.1, SRR1660813.203415.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849387, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAATCTGA GGCTCTGCGT GCAGGCGCTG CTGCTGCTCT GGCTCTCCTT GACTGCAGTT 
TGTGGAGTGC CACTGATGTT GCCTCCAGAT GGAACAGGAC TAGAAGAAGG AAGCATGCGC 
TACCTGGTGA AGCCCAGAAC TTCGAGGACT GGACCAGGAG CCTGGCAGGG AGGCAGGAGG 
AAATTTCGCA GACAGCGCCC CCGGCTCTCC CATAAGGGCC CCATGCCTTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038940.1
CDS	349..582
Translation	MNLRLCVQALLLLWLSLTAVCGVPLMLPPDGTGLEEGSMRYLVKPRTSRTGPGAWQGGRRKFRRQRPRLSHKGPMPF

Target ORF information:

RefSeq Version	NM_013912.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus apelin (Apln), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013912.4

ORF Insert Sequence:

ATGAATCTGA GGCTCTGCGT GCAGGCGCTG CTGCTGCTCT GGCTCTCCTT GACTGCAGTT 
TGTGGAGTGC CACTGATGTT GCCTCCAGAT GGAACAGGAC TAGAAGAAGG AAGCATGCGC 
TACCTGGTGA AGCCCAGAAC TTCGAGGACT GGACCAGGAG CCTGGCAGGG AGGCAGGAGG 
AAATTTCGCA GACAGCGCCC CCGGCTCTCC CATAAGGGCC CCATGCCTTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APLN ( NM_013912.3 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_038940.1 Mus musculus apelin (Apln), mRNA.

Price & Availability↑

CloneID	OMu19425
Clone ID Related Accession (Same CDS sequence)	NM_013912.4 , NM_013912.3
Accession Version	NM_013912.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 234bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-14
Organism	Mus musculus(House mouse)
Product	apelin precursor
Comment

ATGAATCTGA GGCTCTGCGT GCAGGCGCTG CTGCTGCTCT GGCTCTCCTT GACTGCAGTT 
TGTGGAGTGC CACTGATGTT GCCTCCAGAT GGAACAGGAC TAGAAGAAGG AAGCATGCGC 
TACCTGGTGA AGCCCAGAAC TTCGAGGACT GGACCAGGAG CCTGGCAGGG AGGCAGGAGG 
AAATTTCGCA GACAGCGCCC CCGGCTCTCC CATAAGGGCC CCATGCCTTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038940.1
Translation	MNLRLCVQALLLLWLSLTAVCGVPLMLPPDGTGLEEGSMRYLVKPRTSRTGPGAWQGGRRKFRRQRPRLSHKGPMPF

Target ORF information:

RefSeq Version	NM_013912.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus apelin (Apln), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013912.3

ORF Insert Sequence:

ATGAATCTGA GGCTCTGCGT GCAGGCGCTG CTGCTGCTCT GGCTCTCCTT GACTGCAGTT 
TGTGGAGTGC CACTGATGTT GCCTCCAGAT GGAACAGGAC TAGAAGAAGG AAGCATGCGC 
TACCTGGTGA AGCCCAGAAC TTCGAGGACT GGACCAGGAG CCTGGCAGGG AGGCAGGAGG 
AAATTTCGCA GACAGCGCCC CCGGCTCTCC CATAAGGGCC CCATGCCTTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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APLN cDNA ORF clone, Mus musculus(House mouse)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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