Limd1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Limd1
Entrez Gene ID	29806
Full Name	LIM domains containing 1
Synonyms	AW822033,D9Ertd192e
General protein information	Preferred Names LIM domains containing 1 Names LIM domain-containing protein 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9 F4\|9 74.0 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_011242977.2	XP_011241279.1	LIM domain-containing protein 1 isoform X1
XM_011242977.1	XP_011241279.1	LIM domain-containing protein 1 isoform X1
NM_013860.2	NP_038888.2	LIM domain-containing protein 1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Limd1	NP_038888.2
Danio rerio (zebrafish)	LOC562793	XP_691259.5
Pan troglodytes (chimpanzee)	LIMD1	XP_001147894.1
Canis lupus familiaris (dog)	LIMD1	XP_003639802.1
Bos taurus (cattle)	LIMD1	NP_001180092.1
Gallus gallus (chicken)	LIMD1	XP_418800.4
Homo sapiens (human)	LIMD1	NP_055055.1
Rattus norvegicus (Norway rat)	Limd1	NP_001106208.1

Gene Ontology
Bibliography

Related articles in PubMed

TGF? regulates epithelial-mesenchymal interactions through WNT signaling activity to control muscle development in the soft palate.
Iwata J, Suzuki A, Yokota T, Ho TV, Pelikan R, Urata M, Sanchez-Lara PA, Chai Y
Development (Cambridge, England)141(4)909-17(2014 Feb)

Loss of migfilin expression has no overt consequences on murine development and homeostasis.
Moik DV, Janbandhu VC, F?ssler R
Journal of cell science124(Pt 3)414-21(2011 Feb)

Transcription factors expressed in olfactory bulb local progenitor cells revealed by genome-wide transcriptome profiling.
Campbell GR, Baudhuin A, Vranizan K, Ngai J
Molecular and cellular neurosciences46(2)548-61(2011 Feb)

Screening large numbers of expression patterns of transcription factors in late stages of the mouse thymus.
Chung YC, Tsai YJ, Shiu TY, Sun YY, Wang PF, Chen CL
Gene expression patterns : GEP11(1-2)84-92(2011 Jan-Feb)

LIM-domain proteins, LIMD1, Ajuba, and WTIP are required for microRNA-mediated gene silencing.
James V, Zhang Y, Foxler DE, de Moor CH, Kong YW, Webb TM, Self TJ, Feng Y, Lagos D, Chu CY, Rana TM, Morley SJ, Longmore GD, Bushell M, Sharp TV
Proceedings of the National Academy of Sciences of the United States of America107(28)12499-504(2010 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Limd1 influences not only stress osteoclastogenesis but also osteoblast function and osteoblast progenitor commitment. Together, these data identify Limd1 as a novel regulator of both bone osetoclast and bone osteoblast development and function.
Title: The LIM protein LIMD1 influences osteoblast differentiation and function.

Limd1 regulates AP-1 activation through an interaction with Traf6 to influence osteoclast development
Title: The LIM protein, Limd1, regulates AP-1 activation through an interaction with Traf6 to influence osteoclast development.

The following Limd1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Limd1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu69316	XM_011242977.2 Latest version!	Mus musculus LIM domains containing 1 (Limd1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu10067	NM_013860.2 Latest version!	Mus musculus LIM domains containing 1 (Limd1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
View Old Accession Versions >>
OMu69316	XM_011242977.1	Mus musculus LIM domains containing 1 (Limd1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Limd1 ( XM_011242977.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011241279.1 Mus musculus LIM domains containing 1 (Limd1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu69316
Clone ID Related Accession (Same CDS sequence)	XM_011242977.2 , XM_011242977.1
Accession Version	XM_011242977.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1449bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	LIM domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAAT ACGATGATCT GGGCCTTGAG GCCAGTAAGT TCATCGAGGA CCTGAACATG 
TATGAGGCCT CCAAGGATGG GCTCTTCCGG GTGGACAAGG GTGCTGGCAA CAACCCAGAA 
TTTGAGGAAA CTCGAAGGGT GTTCGCGACC AAGATGGCCA AAATCCACCT CCAGCAGCAG 
CAACAGCAGC AGCTCCTACA GGAGGAGGCC CTACCTAGGG CAGGCAGAAG CCCGGTCAAC 
GGTGGGAACC GTCAGGGTGC GAGCGGCAAG CTGGCTGCAG ATGGGGCCGC TAAGCCTCCT 
CTGGCTGTGC CAACAGTGGC ACCTGGATTA GCTACTACCA CTGCGGCCGC GCAGCCCTCG 
TACCCATCTC AGGAGCAGAG GATCAGGCCA TCTGCCCATG GTGCAAGGCC TGGCAGTCAG 
AACTGTGGTT CCAGGGAGGG GCCTGTGAGT TCCCAGAGAC CTGCTTTACA CGGCCTGAGT 
CCCTCCTGTG AAGACCCTTC CTGCCTCACT CACGGAGACT ATTATGACAA TTTCTCTTTG 
GCAAGCCCAC AGTGGGGTGA TAAACCTGAA GGGTGCCCCA GCGTGAGTCT GGGTGTAGGA 
AGTGGATGGC CTGGTTGCCC AGGGAATGAC TCCACATTGC CCAAATCCTG TGGAGACCAT 
CACCCTTACC AGCCACAGCT CTCCACAGTG TGCTCTGGCA GGTCTTTTGA AAGTGGCATC 
AGTGGCCAGG ACGGTGGCAT TGGTGGCCAT AGCAGCGAGA AGCCAACAGG CCTTTGGTCC 
ACTGCCTCCT CTCAGCGAGT GAACCTCGGT TTTTCTTCCA TGGGCTTGGA GAATGGGACC 
TCCGCTCAAC CCAAGGGCAC AACTGTTTCA GCACCGATGG TCCCTAGCAG CGCCAGCCAA 
GGAGCTTGTC CGAAAAGAGA TTCAGGTCTG GGATATGAGG CTTCAGGCAG GGTCTTCAAA 
CCCCTTGTGG ACACTCAGCC TTGGCTGCAG GATGGGCCCA AGTCTTACCT CTCAGTTTCT 
GCTCCATTAT CCTCGACAGC TGGCAAGGAC AGTACCCAGC CAGGTATGAC CACCGGGCTG 
GATCCTAAGT TTGGATGCGT GGAGTCTGGC ACTAGTCCCA AGCCCAGCCC CACCAGTAAC 
GTCCATCCGG TAATGTCCAC TCCATCTGAG TTATCTTGTA AAGAGAGTTC TCCCAGCTGG 
TCCACTGACA GTAGTCTGGA ACCTGTGCTC CCAGGGAGTC CCACCCCCTC CAGGGTGAGA 
TTGCCCTGCC AGACCCTCGC ACCGGGCCCT GAGCTTGGAC CCTCCACTGC GGAATTGAAG 
TTGGAAGCCC TCACCCAGCG TCTGGAGCGA GAGATGGATG CTCACCCCAA AGCGGACTAC 
TTCGATGCTG GTTACAAAAG GAAATCATTA GCCCCTCCCT GGATCTGCTG CAGCAGGAGC 
TCAGGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011241279.1
CDS	578..2026
Translation	MDKYDDLGLEASKFIEDLNMYEASKDGLFRVDKGAGNNPEFEETRRVFATKMAKIHLQQQQQQQLLQEEALPRAGRSPVNGGNRQGASGKLAADGAAKPPLAVPTVAPGLATTTAAAQPSYPSQEQRIRPSAHGARPGSQNCGSREGPVSSQRPALHGLSPSCEDPSCLTHGDYYDNFSLASPQWGDKPEGCPSVSLGVGSGWPGCPGNDSTLPKSCGDHHPYQPQLSTVCSGRSFESGISGQDGGIGGHSSEKPTGLWSTASSQRVNLGFSSMGLENGTSAQPKGTTVSAPMVPSSASQGACPKRDSGLGYEASGRVFKPLVDTQPWLQDGPKSYLSVSAPLSSTAGKDSTQPGMTTGLDPKFGCVESGTSPKPSPTSNVHPVMSTPSELSCKESSPSWSTDSSLEPVLPGSPTPSRVRLPCQTLAPGPELGPSTAELKLEALTQRLEREMDAHPKADYFDAGYKRKSLAPPWICCSRSSG

Target ORF information:

RefSeq Version	XM_011242977.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus LIM domains containing 1 (Limd1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011242977.1

ORF Insert Sequence:

ATGGATAAAT ACGATGATCT GGGCCTTGAG GCCAGTAAGT TCATCGAGGA CCTGAACATG 
TATGAGGCCT CCAAGGATGG GCTCTTCCGG GTGGACAAGG GTGCTGGCAA CAACCCAGAA 
TTTGAGGAAA CTCGAAGGGT GTTCGCGACC AAGATGGCCA AAATCCACCT CCAGCAGCAG 
CAACAGCAGC AGCTCCTACA GGAGGAGGCC CTACCTAGGG CAGGCAGAAG CCCGGTCAAC 
GGTGGGAACC GTCAGGGTGC GAGCGGCAAG CTGGCTGCAG ATGGGGCCGC TAAGCCTCCT 
CTGGCTGTGC CAACAGTGGC ACCTGGATTA GCTACTACCA CTGCGGCCGC GCAGCCCTCG 
TACCCATCTC AGGAGCAGAG GATCAGGCCA TCTGCCCATG GTGCAAGGCC TGGCAGTCAG 
AACTGTGGTT CCAGGGAGGG GCCTGTGAGT TCCCAGAGAC CTGCTTTACA CGGCCTGAGT 
CCCTCCTGTG AAGACCCTTC CTGCCTCACT CACGGAGACT ATTATGACAA TTTCTCTTTG 
GCAAGCCCAC AGTGGGGTGA TAAACCTGAA GGGTGCCCCA GCGTGAGTCT GGGTGTAGGA 
AGTGGATGGC CTGGTTGCCC AGGGAATGAC TCCACATTGC CCAAATCCTG TGGAGACCAT 
CACCCTTACC AGCCACAGCT CTCCACAGTG TGCTCTGGCA GGTCTTTTGA AAGTGGCATC 
AGTGGCCAGG ACGGTGGCAT TGGTGGCCAT AGCAGCGAGA AGCCAACAGG CCTTTGGTCC 
ACTGCCTCCT CTCAGCGAGT GAACCTCGGT TTTTCTTCCA TGGGCTTGGA GAATGGGACC 
TCCGCTCAAC CCAAGGGCAC AACTGTTTCA GCACCGATGG TCCCTAGCAG CGCCAGCCAA 
GGAGCTTGTC CGAAAAGAGA TTCAGGTCTG GGATATGAGG CTTCAGGCAG GGTCTTCAAA 
CCCCTTGTGG ACACTCAGCC TTGGCTGCAG GATGGGCCCA AGTCTTACCT CTCAGTTTCT 
GCTCCATTAT CCTCGACAGC TGGCAAGGAC AGTACCCAGC CAGGTATGAC CACCGGGCTG 
GATCCTAAGT TTGGATGCGT GGAGTCTGGC ACTAGTCCCA AGCCCAGCCC CACCAGTAAC 
GTCCATCCGG TAATGTCCAC TCCATCTGAG TTATCTTGTA AAGAGAGTTC TCCCAGCTGG 
TCCACTGACA GTAGTCTGGA ACCTGTGCTC CCAGGGAGTC CCACCCCCTC CAGGGTGAGA 
TTGCCCTGCC AGACCCTCGC ACCGGGCCCT GAGCTTGGAC CCTCCACTGC GGAATTGAAG 
TTGGAAGCCC TCACCCAGCG TCTGGAGCGA GAGATGGATG CTCACCCCAA AGCGGACTAC 
TTCGATGCTG GTTACAAAAG GAAATCATTA GCCCCTCCCT GGATCTGCTG CAGCAGGAGC 
TCAGGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Limd1 ( XM_011242977.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011241279.1 Mus musculus LIM domains containing 1 (Limd1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu69316
Clone ID Related Accession (Same CDS sequence)	XM_011242977.2 , XM_011242977.1
Accession Version	XM_011242977.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1449bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	LIM domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011242977.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAAT ACGATGATCT GGGCCTTGAG GCCAGTAAGT TCATCGAGGA CCTGAACATG 
TATGAGGCCT CCAAGGATGG GCTCTTCCGG GTGGACAAGG GTGCTGGCAA CAACCCAGAA 
TTTGAGGAAA CTCGAAGGGT GTTCGCGACC AAGATGGCCA AAATCCACCT CCAGCAGCAG 
CAACAGCAGC AGCTCCTACA GGAGGAGGCC CTACCTAGGG CAGGCAGAAG CCCGGTCAAC 
GGTGGGAACC GTCAGGGTGC GAGCGGCAAG CTGGCTGCAG ATGGGGCCGC TAAGCCTCCT 
CTGGCTGTGC CAACAGTGGC ACCTGGATTA GCTACTACCA CTGCGGCCGC GCAGCCCTCG 
TACCCATCTC AGGAGCAGAG GATCAGGCCA TCTGCCCATG GTGCAAGGCC TGGCAGTCAG 
AACTGTGGTT CCAGGGAGGG GCCTGTGAGT TCCCAGAGAC CTGCTTTACA CGGCCTGAGT 
CCCTCCTGTG AAGACCCTTC CTGCCTCACT CACGGAGACT ATTATGACAA TTTCTCTTTG 
GCAAGCCCAC AGTGGGGTGA TAAACCTGAA GGGTGCCCCA GCGTGAGTCT GGGTGTAGGA 
AGTGGATGGC CTGGTTGCCC AGGGAATGAC TCCACATTGC CCAAATCCTG TGGAGACCAT 
CACCCTTACC AGCCACAGCT CTCCACAGTG TGCTCTGGCA GGTCTTTTGA AAGTGGCATC 
AGTGGCCAGG ACGGTGGCAT TGGTGGCCAT AGCAGCGAGA AGCCAACAGG CCTTTGGTCC 
ACTGCCTCCT CTCAGCGAGT GAACCTCGGT TTTTCTTCCA TGGGCTTGGA GAATGGGACC 
TCCGCTCAAC CCAAGGGCAC AACTGTTTCA GCACCGATGG TCCCTAGCAG CGCCAGCCAA 
GGAGCTTGTC CGAAAAGAGA TTCAGGTCTG GGATATGAGG CTTCAGGCAG GGTCTTCAAA 
CCCCTTGTGG ACACTCAGCC TTGGCTGCAG GATGGGCCCA AGTCTTACCT CTCAGTTTCT 
GCTCCATTAT CCTCGACAGC TGGCAAGGAC AGTACCCAGC CAGGTATGAC CACCGGGCTG 
GATCCTAAGT TTGGATGCGT GGAGTCTGGC ACTAGTCCCA AGCCCAGCCC CACCAGTAAC 
GTCCATCCGG TAATGTCCAC TCCATCTGAG TTATCTTGTA AAGAGAGTTC TCCCAGCTGG 
TCCACTGACA GTAGTCTGGA ACCTGTGCTC CCAGGGAGTC CCACCCCCTC CAGGGTGAGA 
TTGCCCTGCC AGACCCTCGC ACCGGGCCCT GAGCTTGGAC CCTCCACTGC GGAATTGAAG 
TTGGAAGCCC TCACCCAGCG TCTGGAGCGA GAGATGGATG CTCACCCCAA AGCGGACTAC 
TTCGATGCTG GTTACAAAAG GAAATCATTA GCCCCTCCCT GGATCTGCTG CAGCAGGAGC 
TCAGGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011241279.1
CDS	578..2026
Translation	MDKYDDLGLEASKFIEDLNMYEASKDGLFRVDKGAGNNPEFEETRRVFATKMAKIHLQQQQQQQLLQEEALPRAGRSPVNGGNRQGASGKLAADGAAKPPLAVPTVAPGLATTTAAAQPSYPSQEQRIRPSAHGARPGSQNCGSREGPVSSQRPALHGLSPSCEDPSCLTHGDYYDNFSLASPQWGDKPEGCPSVSLGVGSGWPGCPGNDSTLPKSCGDHHPYQPQLSTVCSGRSFESGISGQDGGIGGHSSEKPTGLWSTASSQRVNLGFSSMGLENGTSAQPKGTTVSAPMVPSSASQGACPKRDSGLGYEASGRVFKPLVDTQPWLQDGPKSYLSVSAPLSSTAGKDSTQPGMTTGLDPKFGCVESGTSPKPSPTSNVHPVMSTPSELSCKESSPSWSTDSSLEPVLPGSPTPSRVRLPCQTLAPGPELGPSTAELKLEALTQRLEREMDAHPKADYFDAGYKRKSLAPPWICCSRSSG

Target ORF information:

RefSeq Version	XM_011242977.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus LIM domains containing 1 (Limd1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011242977.2

ORF Insert Sequence:

ATGGATAAAT ACGATGATCT GGGCCTTGAG GCCAGTAAGT TCATCGAGGA CCTGAACATG 
TATGAGGCCT CCAAGGATGG GCTCTTCCGG GTGGACAAGG GTGCTGGCAA CAACCCAGAA 
TTTGAGGAAA CTCGAAGGGT GTTCGCGACC AAGATGGCCA AAATCCACCT CCAGCAGCAG 
CAACAGCAGC AGCTCCTACA GGAGGAGGCC CTACCTAGGG CAGGCAGAAG CCCGGTCAAC 
GGTGGGAACC GTCAGGGTGC GAGCGGCAAG CTGGCTGCAG ATGGGGCCGC TAAGCCTCCT 
CTGGCTGTGC CAACAGTGGC ACCTGGATTA GCTACTACCA CTGCGGCCGC GCAGCCCTCG 
TACCCATCTC AGGAGCAGAG GATCAGGCCA TCTGCCCATG GTGCAAGGCC TGGCAGTCAG 
AACTGTGGTT CCAGGGAGGG GCCTGTGAGT TCCCAGAGAC CTGCTTTACA CGGCCTGAGT 
CCCTCCTGTG AAGACCCTTC CTGCCTCACT CACGGAGACT ATTATGACAA TTTCTCTTTG 
GCAAGCCCAC AGTGGGGTGA TAAACCTGAA GGGTGCCCCA GCGTGAGTCT GGGTGTAGGA 
AGTGGATGGC CTGGTTGCCC AGGGAATGAC TCCACATTGC CCAAATCCTG TGGAGACCAT 
CACCCTTACC AGCCACAGCT CTCCACAGTG TGCTCTGGCA GGTCTTTTGA AAGTGGCATC 
AGTGGCCAGG ACGGTGGCAT TGGTGGCCAT AGCAGCGAGA AGCCAACAGG CCTTTGGTCC 
ACTGCCTCCT CTCAGCGAGT GAACCTCGGT TTTTCTTCCA TGGGCTTGGA GAATGGGACC 
TCCGCTCAAC CCAAGGGCAC AACTGTTTCA GCACCGATGG TCCCTAGCAG CGCCAGCCAA 
GGAGCTTGTC CGAAAAGAGA TTCAGGTCTG GGATATGAGG CTTCAGGCAG GGTCTTCAAA 
CCCCTTGTGG ACACTCAGCC TTGGCTGCAG GATGGGCCCA AGTCTTACCT CTCAGTTTCT 
GCTCCATTAT CCTCGACAGC TGGCAAGGAC AGTACCCAGC CAGGTATGAC CACCGGGCTG 
GATCCTAAGT TTGGATGCGT GGAGTCTGGC ACTAGTCCCA AGCCCAGCCC CACCAGTAAC 
GTCCATCCGG TAATGTCCAC TCCATCTGAG TTATCTTGTA AAGAGAGTTC TCCCAGCTGG 
TCCACTGACA GTAGTCTGGA ACCTGTGCTC CCAGGGAGTC CCACCCCCTC CAGGGTGAGA 
TTGCCCTGCC AGACCCTCGC ACCGGGCCCT GAGCTTGGAC CCTCCACTGC GGAATTGAAG 
TTGGAAGCCC TCACCCAGCG TCTGGAGCGA GAGATGGATG CTCACCCCAA AGCGGACTAC 
TTCGATGCTG GTTACAAAAG GAAATCATTA GCCCCTCCCT GGATCTGCTG CAGCAGGAGC 
TCAGGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Limd1 ( NM_013860.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_038888.2 Mus musculus LIM domains containing 1 (Limd1), mRNA.

Price & Availability↑

CloneID	OMu10067
Clone ID Related Accession (Same CDS sequence)	NM_013860.2
Accession Version	NM_013860.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	LIM domain-containing protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY068411.1, BC056449.1, AK047198.1, AJ132409.1 and BE199820.1. On Mar 14, 2009 this sequence version replaced NM_013860.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ132409.1, AK047198.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGATAAAT ACGATGATCT GGGCCTTGAG GCCAGTAAGT TCATCGAGGA CCTGAACATG 
TATGAGGCCT CCAAGGATGG GCTCTTCCGG GTGGACAAGG GTGCTGGCAA CAACCCAGAA 
TTTGAGGAAA CTCGAAGGGT GTTCGCGACC AAGATGGCCA AAATCCACCT CCAGCAGCAG 
CAACAGCAGC AGCTCCTACA GGAGGAGGCC CTACCTAGGG CAGGCAGAAG CCCGGTCAAC 
GGTGGGAACC GTCAGGGTGC GAGCGGCAAG CTGGCTGCAG ATGGGGCCGC TAAGCCTCCT 
CTGGCTGTGC CAACAGTGGC ACCTGGATTA GCTACTACCA CTGCGGCCGC GCAGCCCTCG 
TACCCATCTC AGGAGCAGAG GATCAGGCCA TCTGCCCATG GTGCAAGGCC TGGCAGTCAG 
AACTGTGGTT CCAGGGAGGG GCCTGTGAGT TCCCAGAGAC CTGCTTTACA CGGCCTGAGT 
CCCTCCTGTG AAGACCCTTC CTGCCTCACT CACGGAGACT ATTATGACAA TTTCTCTTTG 
GCAAGCCCAC AGTGGGGTGA TAAACCTGAA GGGTGCCCCA GCGTGAGTCT GGGTGTAGGA 
AGTGGATGGC CTGGTTGCCC AGGGAATGAC TCCACATTGC CCAAATCCTG TGGAGACCAT 
CACCCTTACC AGCCACAGCT CTCCACAGTG TGCTCTGGCA GGTCTTTTGA AAGTGGCATC 
AGTGGCCAGG ACGGTGGCAT TGGTGGCCAT AGCAGCGAGA AGCCAACAGG CCTTTGGTCC 
ACTGCCTCCT CTCAGCGAGT GAACCTCGGT TTTTCTTCCA TGGGCTTGGA GAATGGGACC 
TCCGCTCAAC CCAAGGGCAC AACTGTTTCA GCACCGATGG TCCCTAGCAG CGCCAGCCAA 
GGAGCTTGTC CGAAAAGAGA TTCAGGTCTG GGATATGAGG CTTCAGGCAG GGTCTTCAAA 
CCCCTTGTGG ACACTCAGCC TTGGCTGCAG GATGGGCCCA AGTCTTACCT CTCAGTTTCT 
GCTCCATTAT CCTCGACAGC TGGCAAGGAC AGTACCCAGC CAGGTATGAC CACCGGGCTG 
GATCCTAAGT TTGGATGCGT GGAGTCTGGC ACTAGTCCCA AGCCCAGCCC CACCAGTAAC 
GTCCATCCGG TAATGTCCAC TCCATCTGAG TTATCTTGTA AAGAGAGTTC TCCCAGCTGG 
TCCACTGACA GTAGTCTGGA ACCTGTGCTC CCAGGGAGTC CCACCCCCTC CAGGGTGAGA 
TTGCCCTGCC AGACCCTCGC ACCGGGCCCT GAGCTTGGAC CCTCCACTGC GGAATTGAAG 
TTGGAAGCCC TCACCCAGCG TCTGGAGCGA GAGATGGATG CTCACCCCAA AGCGGACTAC 
TTCGGTTCCT GTGTGAAATG CAGCAAAGGG GTGTTTGGAG CTGGCCAGGC CTGTCAGGCC 
ATGGGAGATC TCTACCACGA TGCGTGCTTC ACCTGTGCAG CCTGCAGCAG GAAGTTAAGA 
GGAAAGGCCT TCTATTTTGT CAATGGCAAA GTATTCTGTG AGGAAGACTT TTTGTATTCT 
GGCTTTCAGC AGTCTGCGGA CAGGTGTTTT CTTTGTGGAC ACCTGATCAT GGACATGATC 
CTACAGGCCC TAGGGAAGTC CTATCACCCC GGCTGTTTCC GCTGCGTCAT CTGTAATGAA 
TGTCTGGATG GGGTTCCTTT CACCGTGGAC TCTGAGAACA AGATCTACTG TGTCCGAGAC 
TATCACAAGG TGCTGGCCCC CAAGTGTGCA GCCTGTGGCC TTCCCATCCT TCCGCCAGAG 
GGTTCAGACG AGACCATCAG AGTTGTGTCC ATGGACAGGG ACTACCACGT AGAGTGCTAC 
CACTGTGAGG ACTGTGGTCT GGAGCTCAAT GACGAGGACG GTCACCGCTG CTATCCACTG 
GAGGACCACC TGTTCTGTCA CTCCTGCCAT GTCAAGAGAC TGGAGAAAGG ACCCTCACCT 
GCACCCCTCC ACCAGCACCA CTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038888.2
CDS	538..2544
Translation	MDKYDDLGLEASKFIEDLNMYEASKDGLFRVDKGAGNNPEFEETRRVFATKMAKIHLQQQQQQQLLQEEALPRAGRSPVNGGNRQGASGKLAADGAAKPPLAVPTVAPGLATTTAAAQPSYPSQEQRIRPSAHGARPGSQNCGSREGPVSSQRPALHGLSPSCEDPSCLTHGDYYDNFSLASPQWGDKPEGCPSVSLGVGSGWPGCPGNDSTLPKSCGDHHPYQPQLSTVCSGRSFESGISGQDGGIGGHSSEKPTGLWSTASSQRVNLGFSSMGLENGTSAQPKGTTVSAPMVPSSASQGACPKRDSGLGYEASGRVFKPLVDTQPWLQDGPKSYLSVSAPLSSTAGKDSTQPGMTTGLDPKFGCVESGTSPKPSPTSNVHPVMSTPSELSCKESSPSWSTDSSLEPVLPGSPTPSRVRLPCQTLAPGPELGPSTAELKLEALTQRLEREMDAHPKADYFGSCVKCSKGVFGAGQACQAMGDLYHDACFTCAACSRKLRGKAFYFVNGKVFCEEDFLYSGFQQSADRCFLCGHLIMDMILQALGKSYHPGCFRCVICNECLDGVPFTVDSENKIYCVRDYHKVLAPKCAACGLPILPPEGSDETIRVVSMDRDYHVECYHCEDCGLELNDEDGHRCYPLEDHLFCHSCHVKRLEKGPSPAPLHQHHF

Target ORF information:

RefSeq Version	NM_013860.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus LIM domains containing 1 (Limd1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013860.2

ORF Insert Sequence:

ATGGATAAAT ACGATGATCT GGGCCTTGAG GCCAGTAAGT TCATCGAGGA CCTGAACATG 
TATGAGGCCT CCAAGGATGG GCTCTTCCGG GTGGACAAGG GTGCTGGCAA CAACCCAGAA 
TTTGAGGAAA CTCGAAGGGT GTTCGCGACC AAGATGGCCA AAATCCACCT CCAGCAGCAG 
CAACAGCAGC AGCTCCTACA GGAGGAGGCC CTACCTAGGG CAGGCAGAAG CCCGGTCAAC 
GGTGGGAACC GTCAGGGTGC GAGCGGCAAG CTGGCTGCAG ATGGGGCCGC TAAGCCTCCT 
CTGGCTGTGC CAACAGTGGC ACCTGGATTA GCTACTACCA CTGCGGCCGC GCAGCCCTCG 
TACCCATCTC AGGAGCAGAG GATCAGGCCA TCTGCCCATG GTGCAAGGCC TGGCAGTCAG 
AACTGTGGTT CCAGGGAGGG GCCTGTGAGT TCCCAGAGAC CTGCTTTACA CGGCCTGAGT 
CCCTCCTGTG AAGACCCTTC CTGCCTCACT CACGGAGACT ATTATGACAA TTTCTCTTTG 
GCAAGCCCAC AGTGGGGTGA TAAACCTGAA GGGTGCCCCA GCGTGAGTCT GGGTGTAGGA 
AGTGGATGGC CTGGTTGCCC AGGGAATGAC TCCACATTGC CCAAATCCTG TGGAGACCAT 
CACCCTTACC AGCCACAGCT CTCCACAGTG TGCTCTGGCA GGTCTTTTGA AAGTGGCATC 
AGTGGCCAGG ACGGTGGCAT TGGTGGCCAT AGCAGCGAGA AGCCAACAGG CCTTTGGTCC 
ACTGCCTCCT CTCAGCGAGT GAACCTCGGT TTTTCTTCCA TGGGCTTGGA GAATGGGACC 
TCCGCTCAAC CCAAGGGCAC AACTGTTTCA GCACCGATGG TCCCTAGCAG CGCCAGCCAA 
GGAGCTTGTC CGAAAAGAGA TTCAGGTCTG GGATATGAGG CTTCAGGCAG GGTCTTCAAA 
CCCCTTGTGG ACACTCAGCC TTGGCTGCAG GATGGGCCCA AGTCTTACCT CTCAGTTTCT 
GCTCCATTAT CCTCGACAGC TGGCAAGGAC AGTACCCAGC CAGGTATGAC CACCGGGCTG 
GATCCTAAGT TTGGATGCGT GGAGTCTGGC ACTAGTCCCA AGCCCAGCCC CACCAGTAAC 
GTCCATCCGG TAATGTCCAC TCCATCTGAG TTATCTTGTA AAGAGAGTTC TCCCAGCTGG 
TCCACTGACA GTAGTCTGGA ACCTGTGCTC CCAGGGAGTC CCACCCCCTC CAGGGTGAGA 
TTGCCCTGCC AGACCCTCGC ACCGGGCCCT GAGCTTGGAC CCTCCACTGC GGAATTGAAG 
TTGGAAGCCC TCACCCAGCG TCTGGAGCGA GAGATGGATG CTCACCCCAA AGCGGACTAC 
TTCGGTTCCT GTGTGAAATG CAGCAAAGGG GTGTTTGGAG CTGGCCAGGC CTGTCAGGCC 
ATGGGAGATC TCTACCACGA TGCGTGCTTC ACCTGTGCAG CCTGCAGCAG GAAGTTAAGA 
GGAAAGGCCT TCTATTTTGT CAATGGCAAA GTATTCTGTG AGGAAGACTT TTTGTATTCT 
GGCTTTCAGC AGTCTGCGGA CAGGTGTTTT CTTTGTGGAC ACCTGATCAT GGACATGATC 
CTACAGGCCC TAGGGAAGTC CTATCACCCC GGCTGTTTCC GCTGCGTCAT CTGTAATGAA 
TGTCTGGATG GGGTTCCTTT CACCGTGGAC TCTGAGAACA AGATCTACTG TGTCCGAGAC 
TATCACAAGG TGCTGGCCCC CAAGTGTGCA GCCTGTGGCC TTCCCATCCT TCCGCCAGAG 
GGTTCAGACG AGACCATCAG AGTTGTGTCC ATGGACAGGG ACTACCACGT AGAGTGCTAC 
CACTGTGAGG ACTGTGGTCT GGAGCTCAAT GACGAGGACG GTCACCGCTG CTATCCACTG 
GAGGACCACC TGTTCTGTCA CTCCTGCCAT GTCAAGAGAC TGGAGAAAGG ACCCTCACCT 
GCACCCCTCC ACCAGCACCA CTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

TGF? regulates epithelial-mesenchymal interactions through WNT signaling activity to control muscle development in the soft palate.
Development (Cambridge, England)141(4)909-17(2014 Feb)
Iwata J,Suzuki A,Yokota T,Ho TV,Pelikan R,Urata M,Sanchez-Lara PA,Chai Y

Loss of migfilin expression has no overt consequences on murine development and homeostasis.
Journal of cell science124(Pt 3)414-21(2011 Feb)
Moik DV,Janbandhu VC,F?ssler R

Transcription factors expressed in olfactory bulb local progenitor cells revealed by genome-wide transcriptome profiling.
Molecular and cellular neurosciences46(2)548-61(2011 Feb)
Campbell GR,Baudhuin A,Vranizan K,Ngai J

Screening large numbers of expression patterns of transcription factors in late stages of the mouse thymus.
Gene expression patterns : GEP11(1-2)84-92(2011 Jan-Feb)
Chung YC,Tsai YJ,Shiu TY,Sun YY,Wang PF,Chen CL

LIM-domain proteins, LIMD1, Ajuba, and WTIP are required for microRNA-mediated gene silencing.
Proceedings of the National Academy of Sciences of the United States of America107(28)12499-504(2010 Jul)
James V,Zhang Y,Foxler DE,de Moor CH,Kong YW,Webb TM,Self TJ,Feng Y,Lagos D,Chu CY,Rana TM,Morley SJ,Longmore GD,Bushell M,Sharp TV

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Limd1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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