Trim36 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Trim36
Entrez Gene ID	28105
Full Name	tripartite motif-containing 36
Synonyms	D18Wsu100e,haprin
General protein information	Preferred Names tripartite motif-containing 36 Names E3 ubiquitin-protein ligase Trim36 RING-type E3 ubiquitin transferase TRIM36 acrosome RBCC protein tripartite motif-containing protein 36
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 18 Map Location: 18 C\|18 24.41 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_178872.4	NP_849203.2	E3 ubiquitin-protein ligase Trim36 isoform 1
NM_001170855.1	NP_001164326.1	E3 ubiquitin-protein ligase Trim36 isoform 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	TRIM36	NP_061170.2
Bos taurus (cattle)	TRIM36	NP_001193655.1
Danio rerio (zebrafish)	trim36	NP_998693.2
Pan troglodytes (chimpanzee)	TRIM36	XP_001146255.1
Rattus norvegicus (Norway rat)	Trim36	NP_001099617.1
Gallus gallus (chicken)	TRIM36	XP_004949742.1
Mus musculus (house mouse)	Trim36	NP_849203.2
	TRIM36	XP_001083279.1
Canis lupus familiaris (dog)	TRIM36	XP_005626376.1
Xenopus tropicalis (tropical clawed frog)	trim36	NP_001039245.1

Gene Ontology
Bibliography

Related articles in PubMed

TRIM46 Controls Neuronal Polarity and Axon Specification by Driving the Formation of Parallel Microtubule Arrays.
van Beuningen SF, Will L, Harterink M, Chazeau A, van Battum EY, Frias CP, Franker MA, Katrukha EA, Stucchi R, Vocking K, Antunes AT, Slenders L, Doulkeridou S, Sillevis Smitt P, Altelaar AF, Post JA, Akhmanova A, Pasterkamp RJ, Kapitein LC, de Graaff E, Hoogenraad CC
Neuron88(6)1208-26(2015 Dec)

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Thompson CL, Ng L, Menon V, Martinez S, Lee CK, Glattfelder K, Sunkin SM, Henry A, Lau C, Dang C, Garcia-Lopez R, Martinez-Ferre A, Pombero A, Rubenstein JLR, Wakeman WB, Hohmann J, Dee N, Sodt AJ, Young R, Smith K, Nguyen TN, Kidney J, Kuan L, Jeromin A, Kaykas A, Miller J, Page D, Orta G, Bernard A, Riley Z, Smith S, Wohnoutka P, Hawrylycz MJ, Puelles L, Jones AR
Neuron83(2)309-323(2014 Jul)

Tudor domain containing 12 (TDRD12) is essential for secondary PIWI interacting RNA biogenesis in mice.
Pandey RR, Tokuzawa Y, Yang Z, Hayashi E, Ichisaka T, Kajita S, Asano Y, Kunieda T, Sachidanandam R, Chuma S, Yamanaka S, Pillai RS
Proceedings of the National Academy of Sciences of the United States of America110(41)16492-7(2013 Oct)

A mutation in the Srrm4 gene causes alternative splicing defects and deafness in the Bronx waltzer mouse.
Nakano Y, Jahan I, Bonde G, Sun X, Hildebrand MS, Engelhardt JF, Smith RJ, Cornell RA, Fritzsch B, B?nfi B
PLoS genetics8(10)e1002966(2012)

Brn3a and Nurr1 mediate a gene regulatory pathway for habenula development.
Quina LA, Wang S, Ng L, Turner EE
The Journal of neuroscience : the official journal of the Society for Neuroscience29(45)14309-22(2009 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

results indicated that the novel RBCC protein Haprin plays a key role in the acrosome reaction and fertilization
Title: Haprin, a novel haploid germ cell-specific RING finger protein involved in the acrosome reaction.

The following Trim36 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Trim36 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu11723	NM_001170855.1 Latest version!	Mus musculus tripartite motif-containing 36 (Trim36), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$419.30 $599.00
OMu07812	NM_178872.4 Latest version!	Mus musculus tripartite motif-containing 36 (Trim36), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$433.30 $619.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Trim36 ( NM_001170855.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001164326.1 Mus musculus tripartite motif-containing 36 (Trim36), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu11723
Clone ID Related Accession (Same CDS sequence)	NM_001170855.1
Accession Version	NM_001170855.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2154bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase Trim36 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC124717.3, CJ046908.1, BC058106.1, CJ301781.1 and BM938922.1. Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGGGG ATGCCTCAGA CTCTCAGGTG ACTATTAAGA ATATCGAAAA GGAGCTTATT 
TGCCCAGCGT GCAAGGAGCT GTTTACCCAC CCGCTGATCC TTCCCTGCCA GCACAGCGTC 
TGTCATAAGT GTGTGAAAGA ACTTTTACTG TCTCTTGATG ACTCGTTCAA TGATGTGGCG 
TCAGACAGCT CAAATCAGAG CAGCCCTAGG CTCCGGCTCA CCTCCCCTAG CATGGATAAA 
ATCGACAAGA TTAACAGACC AGGCTGGAAG CGTAATTCAC TGACCCCGAG ACCAACCACG 
TTCCCTTGTC CTGGCTGTGA ACATGATGTG GATCTTGGAG AGCGAGGAGT CAGTGGTCTG 
TTTCGAAATT TCACTTTGGA AACGATTGTC GAGAGATACC GGCAGGCCGC TAGGGCAGCC 
ACAGCCATTA TGTGTGACCT TTGTAAACCT CCACCCCAGG AATCTACAAA GAGCTGCATG 
GATTGTAGTG CAAGCTACTG CAATGAATGC TTCAAAATTT ATCATCCCTG GGGCACTGTA 
AAAGCCCAGC ATGAGTATGT GGGCCCCACC ACTAATTTCA GACCCAAGGT TCTAATGTGC 
CCAGAACATG AGACAGAGAG AATAAACATG TACTGTGAAC TATGCAGAAG GCCAGTTTGC 
CACCTCTGTA AGTTGGGTGG GAATCATTCC AATCACCGGG TAACCACCAT GAGCAGTGCC 
TACAAAACCT TAAAGGAGAA ACTTTCAAAA GACATTGATT TCCTTATTGG CAAGGAAAGC 
CAGGTGAAGA GTCAAATTTC TGAACTAAAC TTGCTAATGA AAGAGACAGA GTGCAACGGA 
GAGAGGGCGA AGGAAGAAGC GCTGGCGCAT TTTGAAAAGC TCTTTGAAAT CCTGGAAGAC 
AGGAAGTCGT CTGTTCTGAA AGCCATAGAT GCCTCTAAGA AACTAAGACT AGACAAGTTT 
CACACTCAGA TGGAAGAGTA CCAAGGCCTT CTAGAGAATA ACGGGCTCGT GGGGTATGCT 
CAGGAAGTGC TGAAGGAGAC GGATCAGTCT TGCTTTGTGC AGACGGCGAA GCAGCTCCAT 
CTCAGAATAC AGAAAGCTAC GGAGTCCCTG AAGAGCTTTA GACCTGCAGC CCAGGCTTCT 
TTTGAAGACT ATGTTGTTAA CATATCGAAA CAAACAGAGG TGCTTGGAGA GTTGTCCTTT 
TTCTCCAGTG GCATAGACAT TCCTGAGATC AACGAGGAAC AGAGTAAAGT GTATAATAAC 
GCCTTGATAG ACTGGCATCA TCCAGAAAAG GACAAAGCCG ACAGCTATGT TCTAGAATAC 
CGCAAGATTA ATAGAGACGA AGAAATGATA TCATGGAATG AGATAGAAGT TCACGGCACA 
AGTAAAGTTG TCTCCAACCT TGAAAGCAAC AGTCCCTATG CGTTCCGAGT GAGAGCTTAC 
AGGGGTTCTA TCTGCAGTCC CTGCAGCAGA GAATTGATCC TGCATACTCC TCCAGCTCCA 
GTTTTCAGTT TCCTGTTCGA TGAGAAGTGT GGCTACAACA CTGAGCACCT CTTGCTGAAC 
CTGAAGAGAG ACCGGGTGGA GAGCAGAGCT GGATTTAACG TCCTCCTGGC TGCGGAGCGC 
ATCCAAGTGG GCCATTACAC AAGCTTAGAC TACATCATCG GGGATGTCGG AGTCACGAAA 
GGTAAACACT TCTGGGCCTG CCGCGTGGAA CCGTATTCAT ACCTGGTGAA AGTGGGAGTT 
GCTTCCAGCG ACAAACTGCA AGAGTGGCTG CGCTCTCCCC GAGATGCAGC TAGTCCAAGA 
TATGAGCAAG ACAGTGGACA TGACAGTGGA AGCGAGGACG CCTGTTTTGA TTCTTCACAG 
CCCTTTACAT TAGTTACTAT AGGCATGAAG AAATTTTTTA TACCCAAGTC ACCTACTTCC 
TCTAATGAAC CAGAAAACAG AGTTCTCCCC ATGCCAACGA GTATAGGGAT TTTCCTTGAC 
TGTGATAAAG GCAAAGTGAG CTTCTATGAT ATGGACCACA TGAAATGCCT GTATGAGCGC 
CAGGTGGACT GTTCGCATAC AATGTATCCG GCCTTTGCCT TGATGGGCAG CGGAGGAATT 
CAGCTTGAGG AAGCCATCAC AGCAAAGTAT CTGGAATATG AAGAGGATGT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001164326.1
CDS	279..2432
Translation	MEGDASDSQVTIKNIEKELICPACKELFTHPLILPCQHSVCHKCVKELLLSLDDSFNDVASDSSNQSSPRLRLTSPSMDKIDKINRPGWKRNSLTPRPTTFPCPGCEHDVDLGERGVSGLFRNFTLETIVERYRQAARAATAIMCDLCKPPPQESTKSCMDCSASYCNECFKIYHPWGTVKAQHEYVGPTTNFRPKVLMCPEHETERINMYCELCRRPVCHLCKLGGNHSNHRVTTMSSAYKTLKEKLSKDIDFLIGKESQVKSQISELNLLMKETECNGERAKEEALAHFEKLFEILEDRKSSVLKAIDASKKLRLDKFHTQMEEYQGLLENNGLVGYAQEVLKETDQSCFVQTAKQLHLRIQKATESLKSFRPAAQASFEDYVVNISKQTEVLGELSFFSSGIDIPEINEEQSKVYNNALIDWHHPEKDKADSYVLEYRKINRDEEMISWNEIEVHGTSKVVSNLESNSPYAFRVRAYRGSICSPCSRELILHTPPAPVFSFLFDEKCGYNTEHLLLNLKRDRVESRAGFNVLLAAERIQVGHYTSLDYIIGDVGVTKGKHFWACRVEPYSYLVKVGVASSDKLQEWLRSPRDAASPRYEQDSGHDSGSEDACFDSSQPFTLVTIGMKKFFIPKSPTSSNEPENRVLPMPTSIGIFLDCDKGKVSFYDMDHMKCLYERQVDCSHTMYPAFALMGSGGIQLEEAITAKYLEYEEDV

Target ORF information:

RefSeq Version	NM_001170855.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tripartite motif-containing 36 (Trim36), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170855.1

ORF Insert Sequence:

ATGGAGGGGG ATGCCTCAGA CTCTCAGGTG ACTATTAAGA ATATCGAAAA GGAGCTTATT 
TGCCCAGCGT GCAAGGAGCT GTTTACCCAC CCGCTGATCC TTCCCTGCCA GCACAGCGTC 
TGTCATAAGT GTGTGAAAGA ACTTTTACTG TCTCTTGATG ACTCGTTCAA TGATGTGGCG 
TCAGACAGCT CAAATCAGAG CAGCCCTAGG CTCCGGCTCA CCTCCCCTAG CATGGATAAA 
ATCGACAAGA TTAACAGACC AGGCTGGAAG CGTAATTCAC TGACCCCGAG ACCAACCACG 
TTCCCTTGTC CTGGCTGTGA ACATGATGTG GATCTTGGAG AGCGAGGAGT CAGTGGTCTG 
TTTCGAAATT TCACTTTGGA AACGATTGTC GAGAGATACC GGCAGGCCGC TAGGGCAGCC 
ACAGCCATTA TGTGTGACCT TTGTAAACCT CCACCCCAGG AATCTACAAA GAGCTGCATG 
GATTGTAGTG CAAGCTACTG CAATGAATGC TTCAAAATTT ATCATCCCTG GGGCACTGTA 
AAAGCCCAGC ATGAGTATGT GGGCCCCACC ACTAATTTCA GACCCAAGGT TCTAATGTGC 
CCAGAACATG AGACAGAGAG AATAAACATG TACTGTGAAC TATGCAGAAG GCCAGTTTGC 
CACCTCTGTA AGTTGGGTGG GAATCATTCC AATCACCGGG TAACCACCAT GAGCAGTGCC 
TACAAAACCT TAAAGGAGAA ACTTTCAAAA GACATTGATT TCCTTATTGG CAAGGAAAGC 
CAGGTGAAGA GTCAAATTTC TGAACTAAAC TTGCTAATGA AAGAGACAGA GTGCAACGGA 
GAGAGGGCGA AGGAAGAAGC GCTGGCGCAT TTTGAAAAGC TCTTTGAAAT CCTGGAAGAC 
AGGAAGTCGT CTGTTCTGAA AGCCATAGAT GCCTCTAAGA AACTAAGACT AGACAAGTTT 
CACACTCAGA TGGAAGAGTA CCAAGGCCTT CTAGAGAATA ACGGGCTCGT GGGGTATGCT 
CAGGAAGTGC TGAAGGAGAC GGATCAGTCT TGCTTTGTGC AGACGGCGAA GCAGCTCCAT 
CTCAGAATAC AGAAAGCTAC GGAGTCCCTG AAGAGCTTTA GACCTGCAGC CCAGGCTTCT 
TTTGAAGACT ATGTTGTTAA CATATCGAAA CAAACAGAGG TGCTTGGAGA GTTGTCCTTT 
TTCTCCAGTG GCATAGACAT TCCTGAGATC AACGAGGAAC AGAGTAAAGT GTATAATAAC 
GCCTTGATAG ACTGGCATCA TCCAGAAAAG GACAAAGCCG ACAGCTATGT TCTAGAATAC 
CGCAAGATTA ATAGAGACGA AGAAATGATA TCATGGAATG AGATAGAAGT TCACGGCACA 
AGTAAAGTTG TCTCCAACCT TGAAAGCAAC AGTCCCTATG CGTTCCGAGT GAGAGCTTAC 
AGGGGTTCTA TCTGCAGTCC CTGCAGCAGA GAATTGATCC TGCATACTCC TCCAGCTCCA 
GTTTTCAGTT TCCTGTTCGA TGAGAAGTGT GGCTACAACA CTGAGCACCT CTTGCTGAAC 
CTGAAGAGAG ACCGGGTGGA GAGCAGAGCT GGATTTAACG TCCTCCTGGC TGCGGAGCGC 
ATCCAAGTGG GCCATTACAC AAGCTTAGAC TACATCATCG GGGATGTCGG AGTCACGAAA 
GGTAAACACT TCTGGGCCTG CCGCGTGGAA CCGTATTCAT ACCTGGTGAA AGTGGGAGTT 
GCTTCCAGCG ACAAACTGCA AGAGTGGCTG CGCTCTCCCC GAGATGCAGC TAGTCCAAGA 
TATGAGCAAG ACAGTGGACA TGACAGTGGA AGCGAGGACG CCTGTTTTGA TTCTTCACAG 
CCCTTTACAT TAGTTACTAT AGGCATGAAG AAATTTTTTA TACCCAAGTC ACCTACTTCC 
TCTAATGAAC CAGAAAACAG AGTTCTCCCC ATGCCAACGA GTATAGGGAT TTTCCTTGAC 
TGTGATAAAG GCAAAGTGAG CTTCTATGAT ATGGACCACA TGAAATGCCT GTATGAGCGC 
CAGGTGGACT GTTCGCATAC AATGTATCCG GCCTTTGCCT TGATGGGCAG CGGAGGAATT 
CAGCTTGAGG AAGCCATCAC AGCAAAGTAT CTGGAATATG AAGAGGATGT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trim36 ( NM_178872.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_849203.2 Mus musculus tripartite motif-containing 36 (Trim36), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu07812
Clone ID Related Accession (Same CDS sequence)	NM_178872.4
Accession Version	NM_178872.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2190bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase Trim36 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ167273.1, BC058106.1, CJ301781.1 and BM938922.1. On Jan 15, 2010 this sequence version replaced NM_178872.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR1660819.96042.1, SRR1660821.143311.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGGAAT CAGAGGAGAT AAGTGAATTT GGCTACATTA TGGAATTGCT TGCCAAGGGC 
AAGGTGACTA TTAAGAATAT CGAAAAGGAG CTTATTTGCC CAGCGTGCAA GGAGCTGTTT 
ACCCACCCGC TGATCCTTCC CTGCCAGCAC AGCGTCTGTC ATAAGTGTGT GAAAGAACTT 
TTACTGTCTC TTGATGACTC GTTCAATGAT GTGGCGTCAG ACAGCTCAAA TCAGAGCAGC 
CCTAGGCTCC GGCTCACCTC CCCTAGCATG GATAAAATCG ACAAGATTAA CAGACCAGGC 
TGGAAGCGTA ATTCACTGAC CCCGAGACCA ACCACGTTCC CTTGTCCTGG CTGTGAACAT 
GATGTGGATC TTGGAGAGCG AGGAGTCAGT GGTCTGTTTC GAAATTTCAC TTTGGAAACG 
ATTGTCGAGA GATACCGGCA GGCCGCTAGG GCAGCCACAG CCATTATGTG TGACCTTTGT 
AAACCTCCAC CCCAGGAATC TACAAAGAGC TGCATGGATT GTAGTGCAAG CTACTGCAAT 
GAATGCTTCA AAATTTATCA TCCCTGGGGC ACTGTAAAAG CCCAGCATGA GTATGTGGGC 
CCCACCACTA ATTTCAGACC CAAGGTTCTA ATGTGCCCAG AACATGAGAC AGAGAGAATA 
AACATGTACT GTGAACTATG CAGAAGGCCA GTTTGCCACC TCTGTAAGTT GGGTGGGAAT 
CATTCCAATC ACCGGGTAAC CACCATGAGC AGTGCCTACA AAACCTTAAA GGAGAAACTT 
TCAAAAGACA TTGATTTCCT TATTGGCAAG GAAAGCCAGG TGAAGAGTCA AATTTCTGAA 
CTAAACTTGC TAATGAAAGA GACAGAGTGC AACGGAGAGA GGGCGAAGGA AGAAGCGCTG 
GCGCATTTTG AAAAGCTCTT TGAAATCCTG GAAGACAGGA AGTCGTCTGT TCTGAAAGCC 
ATAGATGCCT CTAAGAAACT AAGACTAGAC AAGTTTCACA CTCAGATGGA AGAGTACCAA 
GGCCTTCTAG AGAATAACGG GCTCGTGGGG TATGCTCAGG AAGTGCTGAA GGAGACGGAT 
CAGTCTTGCT TTGTGCAGAC GGCGAAGCAG CTCCATCTCA GAATACAGAA AGCTACGGAG 
TCCCTGAAGA GCTTTAGACC TGCAGCCCAG GCTTCTTTTG AAGACTATGT TGTTAACATA 
TCGAAACAAA CAGAGGTGCT TGGAGAGTTG TCCTTTTTCT CCAGTGGCAT AGACATTCCT 
GAGATCAACG AGGAACAGAG TAAAGTGTAT AATAACGCCT TGATAGACTG GCATCATCCA 
GAAAAGGACA AAGCCGACAG CTATGTTCTA GAATACCGCA AGATTAATAG AGACGAAGAA 
ATGATATCAT GGAATGAGAT AGAAGTTCAC GGCACAAGTA AAGTTGTCTC CAACCTTGAA 
AGCAACAGTC CCTATGCGTT CCGAGTGAGA GCTTACAGGG GTTCTATCTG CAGTCCCTGC 
AGCAGAGAAT TGATCCTGCA TACTCCTCCA GCTCCAGTTT TCAGTTTCCT GTTCGATGAG 
AAGTGTGGCT ACAACACTGA GCACCTCTTG CTGAACCTGA AGAGAGACCG GGTGGAGAGC 
AGAGCTGGAT TTAACGTCCT CCTGGCTGCG GAGCGCATCC AAGTGGGCCA TTACACAAGC 
TTAGACTACA TCATCGGGGA TGTCGGAGTC ACGAAAGGTA AACACTTCTG GGCCTGCCGC 
GTGGAACCGT ATTCATACCT GGTGAAAGTG GGAGTTGCTT CCAGCGACAA ACTGCAAGAG 
TGGCTGCGCT CTCCCCGAGA TGCAGCTAGT CCAAGATATG AGCAAGACAG TGGACATGAC 
AGTGGAAGCG AGGACGCCTG TTTTGATTCT TCACAGCCCT TTACATTAGT TACTATAGGC 
ATGAAGAAAT TTTTTATACC CAAGTCACCT ACTTCCTCTA ATGAACCAGA AAACAGAGTT 
CTCCCCATGC CAACGAGTAT AGGGATTTTC CTTGACTGTG ATAAAGGCAA AGTGAGCTTC 
TATGATATGG ACCACATGAA ATGCCTGTAT GAGCGCCAGG TGGACTGTTC GCATACAATG 
TATCCGGCCT TTGCCTTGAT GGGCAGCGGA GGAATTCAGC TTGAGGAAGC CATCACAGCA 
AAGTATCTGG AATATGAAGA GGATGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_849203.2
CDS	244..2433
Translation	MSESEEISEFGYIMELLAKGKVTIKNIEKELICPACKELFTHPLILPCQHSVCHKCVKELLLSLDDSFNDVASDSSNQSSPRLRLTSPSMDKIDKINRPGWKRNSLTPRPTTFPCPGCEHDVDLGERGVSGLFRNFTLETIVERYRQAARAATAIMCDLCKPPPQESTKSCMDCSASYCNECFKIYHPWGTVKAQHEYVGPTTNFRPKVLMCPEHETERINMYCELCRRPVCHLCKLGGNHSNHRVTTMSSAYKTLKEKLSKDIDFLIGKESQVKSQISELNLLMKETECNGERAKEEALAHFEKLFEILEDRKSSVLKAIDASKKLRLDKFHTQMEEYQGLLENNGLVGYAQEVLKETDQSCFVQTAKQLHLRIQKATESLKSFRPAAQASFEDYVVNISKQTEVLGELSFFSSGIDIPEINEEQSKVYNNALIDWHHPEKDKADSYVLEYRKINRDEEMISWNEIEVHGTSKVVSNLESNSPYAFRVRAYRGSICSPCSRELILHTPPAPVFSFLFDEKCGYNTEHLLLNLKRDRVESRAGFNVLLAAERIQVGHYTSLDYIIGDVGVTKGKHFWACRVEPYSYLVKVGVASSDKLQEWLRSPRDAASPRYEQDSGHDSGSEDACFDSSQPFTLVTIGMKKFFIPKSPTSSNEPENRVLPMPTSIGIFLDCDKGKVSFYDMDHMKCLYERQVDCSHTMYPAFALMGSGGIQLEEAITAKYLEYEEDV

Target ORF information:

RefSeq Version	NM_178872.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus tripartite motif-containing 36 (Trim36), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_178872.4

ORF Insert Sequence:

ATGTCGGAAT CAGAGGAGAT AAGTGAATTT GGCTACATTA TGGAATTGCT TGCCAAGGGC 
AAGGTGACTA TTAAGAATAT CGAAAAGGAG CTTATTTGCC CAGCGTGCAA GGAGCTGTTT 
ACCCACCCGC TGATCCTTCC CTGCCAGCAC AGCGTCTGTC ATAAGTGTGT GAAAGAACTT 
TTACTGTCTC TTGATGACTC GTTCAATGAT GTGGCGTCAG ACAGCTCAAA TCAGAGCAGC 
CCTAGGCTCC GGCTCACCTC CCCTAGCATG GATAAAATCG ACAAGATTAA CAGACCAGGC 
TGGAAGCGTA ATTCACTGAC CCCGAGACCA ACCACGTTCC CTTGTCCTGG CTGTGAACAT 
GATGTGGATC TTGGAGAGCG AGGAGTCAGT GGTCTGTTTC GAAATTTCAC TTTGGAAACG 
ATTGTCGAGA GATACCGGCA GGCCGCTAGG GCAGCCACAG CCATTATGTG TGACCTTTGT 
AAACCTCCAC CCCAGGAATC TACAAAGAGC TGCATGGATT GTAGTGCAAG CTACTGCAAT 
GAATGCTTCA AAATTTATCA TCCCTGGGGC ACTGTAAAAG CCCAGCATGA GTATGTGGGC 
CCCACCACTA ATTTCAGACC CAAGGTTCTA ATGTGCCCAG AACATGAGAC AGAGAGAATA 
AACATGTACT GTGAACTATG CAGAAGGCCA GTTTGCCACC TCTGTAAGTT GGGTGGGAAT 
CATTCCAATC ACCGGGTAAC CACCATGAGC AGTGCCTACA AAACCTTAAA GGAGAAACTT 
TCAAAAGACA TTGATTTCCT TATTGGCAAG GAAAGCCAGG TGAAGAGTCA AATTTCTGAA 
CTAAACTTGC TAATGAAAGA GACAGAGTGC AACGGAGAGA GGGCGAAGGA AGAAGCGCTG 
GCGCATTTTG AAAAGCTCTT TGAAATCCTG GAAGACAGGA AGTCGTCTGT TCTGAAAGCC 
ATAGATGCCT CTAAGAAACT AAGACTAGAC AAGTTTCACA CTCAGATGGA AGAGTACCAA 
GGCCTTCTAG AGAATAACGG GCTCGTGGGG TATGCTCAGG AAGTGCTGAA GGAGACGGAT 
CAGTCTTGCT TTGTGCAGAC GGCGAAGCAG CTCCATCTCA GAATACAGAA AGCTACGGAG 
TCCCTGAAGA GCTTTAGACC TGCAGCCCAG GCTTCTTTTG AAGACTATGT TGTTAACATA 
TCGAAACAAA CAGAGGTGCT TGGAGAGTTG TCCTTTTTCT CCAGTGGCAT AGACATTCCT 
GAGATCAACG AGGAACAGAG TAAAGTGTAT AATAACGCCT TGATAGACTG GCATCATCCA 
GAAAAGGACA AAGCCGACAG CTATGTTCTA GAATACCGCA AGATTAATAG AGACGAAGAA 
ATGATATCAT GGAATGAGAT AGAAGTTCAC GGCACAAGTA AAGTTGTCTC CAACCTTGAA 
AGCAACAGTC CCTATGCGTT CCGAGTGAGA GCTTACAGGG GTTCTATCTG CAGTCCCTGC 
AGCAGAGAAT TGATCCTGCA TACTCCTCCA GCTCCAGTTT TCAGTTTCCT GTTCGATGAG 
AAGTGTGGCT ACAACACTGA GCACCTCTTG CTGAACCTGA AGAGAGACCG GGTGGAGAGC 
AGAGCTGGAT TTAACGTCCT CCTGGCTGCG GAGCGCATCC AAGTGGGCCA TTACACAAGC 
TTAGACTACA TCATCGGGGA TGTCGGAGTC ACGAAAGGTA AACACTTCTG GGCCTGCCGC 
GTGGAACCGT ATTCATACCT GGTGAAAGTG GGAGTTGCTT CCAGCGACAA ACTGCAAGAG 
TGGCTGCGCT CTCCCCGAGA TGCAGCTAGT CCAAGATATG AGCAAGACAG TGGACATGAC 
AGTGGAAGCG AGGACGCCTG TTTTGATTCT TCACAGCCCT TTACATTAGT TACTATAGGC 
ATGAAGAAAT TTTTTATACC CAAGTCACCT ACTTCCTCTA ATGAACCAGA AAACAGAGTT 
CTCCCCATGC CAACGAGTAT AGGGATTTTC CTTGACTGTG ATAAAGGCAA AGTGAGCTTC 
TATGATATGG ACCACATGAA ATGCCTGTAT GAGCGCCAGG TGGACTGTTC GCATACAATG 
TATCCGGCCT TTGCCTTGAT GGGCAGCGGA GGAATTCAGC TTGAGGAAGC CATCACAGCA 
AAGTATCTGG AATATGAAGA GGATGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

TRIM46 Controls Neuronal Polarity and Axon Specification by Driving the Formation of Parallel Microtubule Arrays.
Neuron88(6)1208-26(2015 Dec)
van Beuningen SF,Will L,Harterink M,Chazeau A,van Battum EY,Frias CP,Franker MA,Katrukha EA,Stucchi R,Vocking K,Antunes AT,Slenders L,Doulkeridou S,Sillevis Smitt P,Altelaar AF,Post JA,Akhmanova A,Pasterkamp RJ,Kapitein LC,de Graaff E,Hoogenraad CC

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Neuron83(2)309-323(2014 Jul)
Thompson CL,Ng L,Menon V,Martinez S,Lee CK,Glattfelder K,Sunkin SM,Henry A,Lau C,Dang C,Garcia-Lopez R,Martinez-Ferre A,Pombero A,Rubenstein JLR,Wakeman WB,Hohmann J,Dee N,Sodt AJ,Young R,Smith K,Nguyen TN,Kidney J,Kuan L,Jeromin A,Kaykas A,Miller J,Page D,Orta G,Bernard A,Riley Z,Smith S,Wohnoutka P,Hawrylycz MJ,Puelles L,Jones AR

Tudor domain containing 12 (TDRD12) is essential for secondary PIWI interacting RNA biogenesis in mice.
Proceedings of the National Academy of Sciences of the United States of America110(41)16492-7(2013 Oct)
Pandey RR,Tokuzawa Y,Yang Z,Hayashi E,Ichisaka T,Kajita S,Asano Y,Kunieda T,Sachidanandam R,Chuma S,Yamanaka S,Pillai RS

A mutation in the Srrm4 gene causes alternative splicing defects and deafness in the Bronx waltzer mouse.
PLoS genetics8(10)e1002966(2012)
Nakano Y,Jahan I,Bonde G,Sun X,Hildebrand MS,Engelhardt JF,Smith RJ,Cornell RA,Fritzsch B,B?nfi B

Brn3a and Nurr1 mediate a gene regulatory pathway for habenula development.
The Journal of neuroscience : the official journal of the Society for Neuroscience29(45)14309-22(2009 Nov)
Quina LA,Wang S,Ng L,Turner EE

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Trim36 cDNA ORF clone, Mus musculus(house mouse)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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