gRNAs
The following Prpf19 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prpf19 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OMu15841 | |
| Clone ID Related Accession (Same CDS sequence) | NM_134129.4 | |
| Accession Version | NM_134129.4 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1515bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-27 | |
| Organism | Mus musculus(house mouse) | |
| Product | pre-mRNA-processing factor 19 isoform 2 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC132402.3 and AK164681.1. On Dec 8, 2011 this sequence version replaced NM_134129.3. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the central coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK164681.1, AK088769.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGTCCCTGA TCTGCTCGAT CTCCAATGAA GTGCCAGAGC ACCCGTGCGT GTCCCCTGTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_598890.1 |
| CDS | 220..1734 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_134129.4 |
1 | ATGTCCCTGA TCTGCTCGAT CTCCAATGAA GTGCCAGAGC ACCCGTGCGT GTCCCCTGTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu05657 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001253844.1 | |
| Accession Version | NM_001253844.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1260bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-02-22 | |
| Organism | Mus musculus(house mouse) | |
| Product | pre-mRNA-processing factor 19 isoform 3 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC132402.3 and AK076998.1. Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (3) has a distinct N-terminus and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK076998.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGCTGCACA GCTTCACTCT TCGCCAGCAA CTGCAGACAA CCCGCCAGGA GCTGTCCCAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001240773.1 |
| CDS | 125..1384 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001253844.1 |
1 | ATGCTGCACA GCTTCACTCT TCGCCAGCAA CTGCAGACAA CCCGCCAGGA GCTGTCCCAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu05656 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001253843.1 | |
| Accession Version | NM_001253843.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1572bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-02-22 | |
| Organism | Mus musculus(house mouse) | |
| Product | pre-mRNA-processing factor 19 isoform 1 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC132402.3 and AK047693.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK047693.1, AB222602.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGTCCCTGA TCTGCTCGAT CTCCAATGAA GTGCCAGAGC ACCCGTGCGT GTCCCCTGTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001240772.1 |
| CDS | 220..1791 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001253843.1 |
1 | ATGTCCCTGA TCTGCTCGAT CTCCAATGAA GTGCCAGAGC ACCCGTGCGT GTCCCCTGTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
SNEV(Prp19/PSO4) deficiency increases PUVA-induced senescence in mouse skin. |
A proteomic comparison of immature and mature mouse gonadotrophs reveals novel differentially expressed nuclear proteins that regulate gonadotropin gene transcription and RNA splicing. |
Haploinsufficiency of senescence evasion factor causes defects of hematopoietic stem cells functions. |
Identification of the domains required for the localization of Prp19p to lipid droplets or the nucleus. |
Mouse homologue of yeast Prp19 interacts with mouse SUG1, the regulatory subunit of 26S proteasome. |
