Shroom3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Shroom3
Entrez Gene ID	27428
Full Name	shroom family member 3
Synonyms	AL022960,D5Ertd287e,Shrm,Shrm3
General protein information	Preferred Names shroom family member 3 Names protein Shroom3
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5 E2\|5 47.29 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006534968.2	XP_006535031.1	protein Shroom3 isoform X2
NM_015756.2	NP_056571.2	protein Shroom3 isoform 1
NM_001077595.2	NP_001071063.1	protein Shroom3 isoform 2
XM_006534966.3	XP_006535029.1	protein Shroom3 isoform X1
XM_006534966.4	XP_006535029.1	protein Shroom3 isoform X1
NM_001077596.2	NP_001071064.1	protein Shroom3 isoform 2
XM_006534968.3	XP_006535031.1	protein Shroom3 isoform X2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	SHROOM3	NP_001178453.1
Rattus norvegicus (Norway rat)	Shroom3	NP_001094359.1
Gallus gallus (chicken)	SHROOM3	XP_420592.4
Pan troglodytes (chimpanzee)	SHROOM3	XP_003950378.1
	SHROOM3	XP_001094783.2
Canis lupus familiaris (dog)	SHROOM3	XP_005639139.1
Mus musculus (house mouse)	Shroom3	NP_056571.2
Xenopus tropicalis (tropical clawed frog)	shroom3	XP_002938160.2
Homo sapiens (human)	SHROOM3	NP_065910.3

Gene Ontology
Bibliography

Related articles in PubMed

Lens placode planar cell polarity is dependent on Cdc42-mediated junctional contraction inhibition.
Muccioli M, Qaisi D, Herman K, Plageman TF Jr
Developmental biology412(1)32-43(2016 Apr)

Thyroid bud morphogenesis requires CDC42- and SHROOM3-dependent apical constriction.
Loebel DA, Plageman TF Jr, Tang TL, Jones VJ, Muccioli M, Tam PP
Biology open5(2)130-9(2016 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Shroom3 has a role in apical constriction during lens pit morphogenesis and is recruited in a pathway involving p120-catenin
Title: p120-catenin-dependent junctional recruitment of Shroom3 is required for apical constriction during lens pit morphogenesis.

findings reveal that Lulu is essential for Shroom3-dependent apical constriction during vertebrate neural tube closure
Title: Lulu regulates Shroom-induced apical constriction during neural tube closure.

Shroom3 and N-cadherin function cooperatively downstream of Pitx2 to directly regulate cell shape changes necessary for early gut tube morphogenesis
Title: Shroom3 and a Pitx2-N-cadherin pathway function cooperatively to generate asymmetric cell shape changes during gut morphogenesis.

Scaffold protein POSH, in association with Shroom3 and mixed lineage kinase LZK, relays axon outgrowth inhibition downstream of NogoA and PirB proteins.
Title: POSH is an intracellular signal transducer for the axon outgrowth inhibitor Nogo66.

expression of Shroom3 is dependent on the crucial lens-induction transcription factor Pax6
Title: Pax6-dependent Shroom3 expression regulates apical constriction during lens placode invagination.

The following Shroom3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Shroom3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu01300	XM_006534968.3 Latest version!	Mus musculus shroom family member 3 (Shroom3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu35489	XM_006534966.4 Latest version!	Mus musculus shroom family member 3 (Shroom3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu01300	NM_001077596.2 Latest version!	Mus musculus shroom family member 3 (Shroom3), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OMu01300	NM_001077595.2 Latest version!	Mus musculus shroom family member 3 (Shroom3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OMu10221	NM_015756.2 Latest version!	Mus musculus shroom family member 3 (Shroom3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
View Old Accession Versions >>
OMu35489	XM_006534966.3	Mus musculus shroom family member 3 (Shroom3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OMu01300	XM_006534968.2	Mus musculus shroom family member 3 (Shroom3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Shroom3 ( XM_006534968.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006535031.1 Mus musculus shroom family member 3 (Shroom3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu01300
Clone ID Related Accession (Same CDS sequence)	XM_006534968.2 , NM_001077595.2 , NM_001077596.2 , XM_006534968.3
Accession Version	XM_006534968.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5418bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein Shroom3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006534968.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCAGA TATCTCAGGG CACAATGGGC CCTCCTTGGC ACCAGAGCTA CCATTCCAGC 
TCTTCGACCA GTGACCTCTC CAACTACGAC CATGCTTATC TGAGGCGGAG CCCTGACCAG 
TGCAGCTCCC AGGGGAGCAT GGAAAGCCTG GAGCCCAGTG GCGGATACCC ACCCTGCCAT 
CTCCTTTCTC CTGCCAAGTC CACGAGCAGC ATCGACCAGC TAGGCCACCT TCATAACAAA 
AGGGACTCCG CATATAGCTC CTTCTCCACC AGTTCGAGCA TCTTCGAGTA CCCACCCCCT 
GGTGGCTCTG CCCGAGAACG TTCGGGTTCC ATGGACGTGA TTTCTGCCCG GGGTGGCCTC 
CTAGAAGGGA TGAGGCAGGC AGACATCCGC TACGTCAAGA CGGTCTATGA TACCCGGAGA 
GGAGTATCTT CAGAGTATGA GGTGAACCCT TCAGCCTTGC TCCTTCAAGG TAGGGATGCC 
CATGCCTCAG CCGACAGTCA GGGCTGTGCT AAATGGCACA GCATTCCTCG GGGCAAGGGA 
ACGCCTTCCC CGTCTTGGAG CCAGCAGTGT TCTGGTTCCC TGGAGACTGC TACTGACAAC 
CTGCCCCAGA AGGCAGGCGC ACCCCTGCCC CCCACCCGCA GTGATAGCTA TGCAGCCTTC 
CGCCATCGCG AGCGTCCCAG CTCCTGGTCC AGCCTTGATC AGAAAAGGTT CTGCCGGCCT 
CAGACAAACT CTTCAGGCTC CCAGAAAACC CCATTCGCTG AGGACCAGCT GCATACAGTC 
CCAGAGAGGA GTCCAGAGAA CAGCCCCCCA GTGAAATCTA AGCATAATTA TACCCAGAAG 
GCGCAGCCTG GCCAACCTCT GCTGCCCACT GGCATCTATC CGGTACCTTC TCCGGAGCCT 
CACTTTGCCC AGGTGCCCCA GCCCTCTGTG AGTAGTAATG GCACCGTCTA CCCTGCGCTA 
GTCAAAGAGA GTGGATACAC AGCTGCTCAG GGAACCTGTA ACAAGATGGC TACCTTAGAT 
GAGAACGGGA ATCAAAATGA AGCTAGCAGG CCTGGGTTTG CCTTCTGTCA GCCCCTAGAA 
CACAACTCGG TGACTCCGGT GGAGAAAAGG CCAGAACCCA CGGCCAAATA CATCTACAAA 
GTCCATTTTT CTTCAGTGCC TGAAAATGAA GACTCCTCCC TAAAGAGGCA CATCACACCT 
CCCCACGGCC ATAGTCCATA TCCCAGCGAG AGAAAGAACA TCCACGGTGG CAGCAGAGCG 
TGTTCCAATC ACCACAGTCT CTCGTCCCCC CAGGCCCAGG CCCTGCACGT GGGTGATGAC 
AGGAGGCCTT CCAGGCTCTC TCAGCCCTGG GAGGGTGACT TTCAGGAAGA TCACAATGCC 
AACCTCAGGC AGAAAGTGGA GAGGGAGGGC CAAGGCCAGG GCCTGTCGGG CAACTCTGGC 
AGGACCAGAT CGGCCTTCTC ATCTCTGCAG AATATTCCAG AGAGTCTGAG AAGACAAAGC 
AACGTGGAGC TTGGAGAAGC CCAGGAGGTT CACCCCGGGG GCAGGTCCAA GGTGGAGGAC 
CCTGGAAGGA AAGCAGGGGC TTCAGACATC AGGGGCTACC TGGACAGATC TGTTTCCTAT 
CCGAGGCCCG AGGGGAAAAT GAACGCCGTA GACTCCGTCC ACAGTGCAGA CTCCAGGTAT 
GAGGAGTCCC CGGCCCCAGC GCTGCCACAG ACATCCGGTG CGAGCCAGCG GCGGCTCAGC 
TCCAGCAGCT CCGCCGCCCC GCAGTACAGG AAGCCCCATT GCTCCGTGCT GGAGAAGGTT 
TCTAGAATAG AGGAACGAGA GCAGGGGCGC CACAGACCCC TGAGCGTGGG CAGCTCTGCA 
TACGGCCCCG GCTACAGGCC TGGGAGGACC GGCCCGACCC CCAGTACTTC CAGCAGTGAC 
CTCGACGATC CAAAAGCCGG CAGCGTCCAT TTCTCGGAGT CCACTGAACA CCTGCGCAAT 
GGGGAGCAGA ACCCCCCAAA CGGGGAGGCT AAGCAGGAAG AGGCTTCCCG GCCCCAGTGC 
AGCCATTTGA TCCGGAGGGC CCCTGCGGAC GGCCGTGGAC CTCCTGCCCG AGGCGGCGAG 
CCCTCAAGGC CGGAGGCCCG CCTGCTCCGC AGCCAGAGCA CCTTTCAGCT CTACAGTGAG 
GCGGAGAGGG AAGCCTCGTG GTCAGAGGAC AGGCCCGGTA CCCCTGAGTC GCCCCTGCTC 
GATGCGCCTT TCAGCCGCGC CTACAGAAAC AGCATCAAGG ATGCCCAGTC CCGCGTCCTG 
GGGGCCACGT CGTTTCGTCG CCGAGACCTG GAACCGGGCA CCCCCGCCAC CTCGAGGCCC 
TGGCGCCCGC GACCCGCCTC CGCCCACGTG GGGATGCGGA GCCCCGAGGC CGCGGTGCCC 
TCGTCCTCCC CGCACACCCC TCGGGAGCGA CACAGCGTGA CCCCGGCCGC GCCCCAGGCT 
GCCCGCCGAG GCCCACGCCG GCGCCTCACT GTCGAGCAGA AGAAGCGCTC CTACTCGGAG 
CCCGAGAAGA TGAACGAGGT AGGGGTCTCG GAGGAGGCCG AGCCCACGCC CTGCGGCCCG 
CCGAGGCCCG CGCAGCCGCG CTTCTCGGAG AGCACCGTGG CCGACCGGCG CCGCATCTTC 
GAGCGCGACG GCAAGGCCTG CTCCACGCTC AGCCTGTCGG GCCCCGAGCT CAAGCAGTTC 
CAGCAGAGCG CCCTGGCCGA CTACATCCAG CGTAAGACGG GCAAGCGGCC CACAGGAGCC 
GCCTGCACAC CGGAGGCCGG GCTGCGCGAG CGCGCACAGA GCGCCTACCT CCAGGCCGGC 
CCCGCGGCGC CCGATGGCCC CGGCCTGGCC TCTGCCTGTA GCCTGAGCTC CCTGCGAGAG 
CCCGAGGCGC TGCCCCGCAA GGAGCACACT CATCCGTCGG CCGCGGACGG CCCGCAGGCG 
CCCCGTGATC GCAGCAGCTC CTTCGCCAGC GGACGCCTAG TCGGGGAGCG ACGTCGCTGG 
GACCCTCAGG TCCCCAGGCA GCTGCTCAGC GGAGCGAACT GCGAACCGAG GGGCGTCCAG 
AGGATGGACG GGGCCCCGGG CGGGCCGCCT TCCTGGGGCA TGGTGGCCGG GAAGGCCGGG 
AAGTCCAAGT CAGCCGAGGA TCTGCTGGAG CGCTCCGACA CACTGGCGGT CCCTGTGCAT 
GTGAGGTCCA GGTCGTCTCC CACCTCCGAC AAGAAAGGTC AGGATGTGCT CCTGAGGGAA 
GGCAGCAACT TTGGTTTTGT AAAGGATCCA TGCTGTTTGG CTGGCCCTGG ACCTAGGTCT 
CTCAGCTGCT CAGACAAAGG CCAGAATGAG CTGGCATTGC CCCTGCACCA CCCTACGCCC 
TGCTGGAACG GCTCAGGCTG CAAAGCCACT GTGGCTTCCT CAGCTCCTCC CGAATCTTCT 
GGAGCCGCGG ATCATCTGAA GCAACGTAGA GCGCCTGGTC CTAGGCCACT CTCAGCAGGA 
ATGCACGGCC ACTTCCCAGA CGCCAGAGCT GCCTCACTCA GCTCCCCTCT GCCATCACCT 
GTCCCCTCCA GTTACCGGTC ACAGCTTGCC ATGGATCAGC AGACTGGGCA ACAGCCTCCC 
TCATCCCCTG CCTCTGCAGT GACCCAGCCT ACCAGTCCAA GAAGCCCTGA GCTCAGCAGC 
CCAGCATATG GGCTAGGGGA GGGGATGTGG AAGAGGACAT CCCTGCCTCA GCGACCCCCT 
CCCCCTTGGG TGAAATGGGC CCACGCGGTC AGAGAGGACG GCCTTGCTGA GGACACCTTG 
GCGCCTGAGT TTGCGAACCT GAAGCACTAT AGAAACCAGC CGAGTCGTCC GAGTTCCTGC 
AGCACTTCTG ACCCAGACAC TCCAGGAAGG ATCTCGCTCC GAATATCGGA GTCAGCCCTT 
CAACCTTCCC CACCGCCCCG GGGGGACTAT GATGACGAAG TGTTCATGAA GGACTTGCAC 
CCCAAGGTTA CTTCAAGCCC CACATTTGAA GCTCTTCCCC CACCCCCGCC ACCTTCGCCA 
CCGAGCGAGG AACCTCTAGT GAATGGCACA GATGACTTTC CTCCGCCTCC TCCTCCCCAA 
GCCCTGTGTG AGGTACTGCT GGATGGTGAG GCATCCACGG AAGCTGGCAG CGGCCCCTGC 
AGAATTCCCA GGGTGATGGT CACAAGGGAA GGGCACGTGC CTGGGGCAGC CCATTCCGAA 
GGTAGTCAGA TAATGACTGC CACACCACCC CAAACCTCGG CCAAAGGTTC AGAAGCCGAG 
TCCAACACGC CTTCCAGCGC GAGCGCCCAG CCTCAGCTCA ACGGTTCTCC TGGCAAGCAG 
CTCTGTCCCA GCCAGACCCG AAACCTCACT TATGAACCTG TCGAGAGAAC TCAGGATCTA 
GGAAAGAAAA CTCACGCCGA GCCTCAGAAG ACCTCAGAAG ATATCCGGAC AGAGGCTCTA 
GCCAAGGAAA TTGTCCACCA AGACAAGTCT CTGGCAGACA TTTTAGATCC TGACTCCAGG 
ATGAAGACAA CGATGGACCT GATGGAGGGT TTATTCCCTG GAGACGCCAG TGTGCTGATG 
GATAGTGGCG CAAAGAGGAA AGCCTTAGAC ATCACCGCCA GGCGTGCGGG ATGTGAAGCC 
AAGGCGAGCG ACCACAAGGA GGCAGTGAGT GTGCTGGTCA ACTGTCCTGC CTACTACAGT 
GTCTCTGCTG CCAAGGCGGA GCTGCTCAAC AAAATCAAAG ACATGCCGGA GGAGCTGCAG 
GAGGAGGAGG GGCAGGAGGA TGTCAATGAG AAAAAGGCTG AGCTTATCGG AAGCCTTACC 
CACAAGCTGG AGAGCCTCCA GGAAGCCAAG GGGAGCCTGC TCACGGACAT CAAGCTGAAC 
AATGCCCTGG GAGAGGAGGT GGAGGCTCTG ATCAGTGAGC TCTGCAAACC CAACGAGTTT 
GACAAGTACA AGATGTTCAT AGGGGACCTG GACAAGGTGG TCAACCTGCT GCTGTCCCTG 
TCTGGACGCC TGGCCCGCGT GGAGAACGTC CTCAGAGGCC TTGGTGAAGA CGCCAGCAAA 
GAGGAAAGGA GTTCTCTGAA CGAGAAAAGG AAGGTTCTGG CTGGCCAGCA TGAGGATGCA 
CGGGAGCTCA AGGAGAACCT GGACCGGAGG GAGCGCGTGG TGCTGGATAT CCTGGCCAAC 
TACCTGTCCG CCGAGCAGCT GCAGGACTAC CAGCACTTCG TGAAGATGAA GTCCACACTC 
CTCATTGAGC AGCGGAAGCT GGATGACAAG ATAAAGCTGG GCCAGGAGCA GGTCAGGTGC 
CTGCTGGAGT CGCTTCCCTC GGACTTCAGG CCCAAGGCAG GGGCCATCTC CCTCCCTCCA 
GCCCTCACCG GCCATGGGAC TCCTGGGGGG ACATCTGTAT TCGGTGGTGT TTTCCCAACG 
TTGACCTCTC CTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006535031.1
CDS	165..5582
Translation	MMQISQGTMGPPWHQSYHSSSSTSDLSNYDHAYLRRSPDQCSSQGSMESLEPSGGYPPCHLLSPAKSTSSIDQLGHLHNKRDSAYSSFSTSSSIFEYPPPGGSARERSGSMDVISARGGLLEGMRQADIRYVKTVYDTRRGVSSEYEVNPSALLLQGRDAHASADSQGCAKWHSIPRGKGTPSPSWSQQCSGSLETATDNLPQKAGAPLPPTRSDSYAAFRHRERPSSWSSLDQKRFCRPQTNSSGSQKTPFAEDQLHTVPERSPENSPPVKSKHNYTQKAQPGQPLLPTGIYPVPSPEPHFAQVPQPSVSSNGTVYPALVKESGYTAAQGTCNKMATLDENGNQNEASRPGFAFCQPLEHNSVTPVEKRPEPTAKYIYKVHFSSVPENEDSSLKRHITPPHGHSPYPSERKNIHGGSRACSNHHSLSSPQAQALHVGDDRRPSRLSQPWEGDFQEDHNANLRQKVEREGQGQGLSGNSGRTRSAFSSLQNIPESLRRQSNVELGEAQEVHPGGRSKVEDPGRKAGASDIRGYLDRSVSYPRPEGKMNAVDSVHSADSRYEESPAPALPQTSGASQRRLSSSSSAAPQYRKPHCSVLEKVSRIEEREQGRHRPLSVGSSAYGPGYRPGRTGPTPSTSSSDLDDPKAGSVHFSESTEHLRNGEQNPPNGEAKQEEASRPQCSHLIRRAPADGRGPPARGGEPSRPEARLLRSQSTFQLYSEAEREASWSEDRPGTPESPLLDAPFSRAYRNSIKDAQSRVLGATSFRRRDLEPGTPATSRPWRPRPASAHVGMRSPEAAVPSSSPHTPRERHSVTPAAPQAARRGPRRRLTVEQKKRSYSEPEKMNEVGVSEEAEPTPCGPPRPAQPRFSESTVADRRRIFERDGKACSTLSLSGPELKQFQQSALADYIQRKTGKRPTGAACTPEAGLRERAQSAYLQAGPAAPDGPGLASACSLSSLREPEALPRKEHTHPSAADGPQAPRDRSSSFASGRLVGERRRWDPQVPRQLLSGANCEPRGVQRMDGAPGGPPSWGMVAGKAGKSKSAEDLLERSDTLAVPVHVRSRSSPTSDKKGQDVLLREGSNFGFVKDPCCLAGPGPRSLSCSDKGQNELALPLHHPTPCWNGSGCKATVASSAPPESSGAADHLKQRRAPGPRPLSAGMHGHFPDARAASLSSPLPSPVPSSYRSQLAMDQQTGQQPPSSPASAVTQPTSPRSPELSSPAYGLGEGMWKRTSLPQRPPPPWVKWAHAVREDGLAEDTLAPEFANLKHYRNQPSRPSSCSTSDPDTPGRISLRISESALQPSPPPRGDYDDEVFMKDLHPKVTSSPTFEALPPPPPPSPPSEEPLVNGTDDFPPPPPPQALCEVLLDGEASTEAGSGPCRIPRVMVTREGHVPGAAHSEGSQIMTATPPQTSAKGSEAESNTPSSASAQPQLNGSPGKQLCPSQTRNLTYEPVERTQDLGKKTHAEPQKTSEDIRTEALAKEIVHQDKSLADILDPDSRMKTTMDLMEGLFPGDASVLMDSGAKRKALDITARRAGCEAKASDHKEAVSVLVNCPAYYSVSAAKAELLNKIKDMPEELQEEEGQEDVNEKKAELIGSLTHKLESLQEAKGSLLTDIKLNNALGEEVEALISELCKPNEFDKYKMFIGDLDKVVNLLLSLSGRLARVENVLRGLGEDASKEERSSLNEKRKVLAGQHEDARELKENLDRRERVVLDILANYLSAEQLQDYQHFVKMKSTLLIEQRKLDDKIKLGQEQVRCLLESLPSDFRPKAGAISLPPALTGHGTPGGTSVFGGVFPTLTSPL

Target ORF information:

RefSeq Version	XM_006534968.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus shroom family member 3 (Shroom3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006534968.3

ORF Insert Sequence:

ATGATGCAGA TATCTCAGGG CACAATGGGC CCTCCTTGGC ACCAGAGCTA CCATTCCAGC 
TCTTCGACCA GTGACCTCTC CAACTACGAC CATGCTTATC TGAGGCGGAG CCCTGACCAG 
TGCAGCTCCC AGGGGAGCAT GGAAAGCCTG GAGCCCAGTG GCGGATACCC ACCCTGCCAT 
CTCCTTTCTC CTGCCAAGTC CACGAGCAGC ATCGACCAGC TAGGCCACCT TCATAACAAA 
AGGGACTCCG CATATAGCTC CTTCTCCACC AGTTCGAGCA TCTTCGAGTA CCCACCCCCT 
GGTGGCTCTG CCCGAGAACG TTCGGGTTCC ATGGACGTGA TTTCTGCCCG GGGTGGCCTC 
CTAGAAGGGA TGAGGCAGGC AGACATCCGC TACGTCAAGA CGGTCTATGA TACCCGGAGA 
GGAGTATCTT CAGAGTATGA GGTGAACCCT TCAGCCTTGC TCCTTCAAGG TAGGGATGCC 
CATGCCTCAG CCGACAGTCA GGGCTGTGCT AAATGGCACA GCATTCCTCG GGGCAAGGGA 
ACGCCTTCCC CGTCTTGGAG CCAGCAGTGT TCTGGTTCCC TGGAGACTGC TACTGACAAC 
CTGCCCCAGA AGGCAGGCGC ACCCCTGCCC CCCACCCGCA GTGATAGCTA TGCAGCCTTC 
CGCCATCGCG AGCGTCCCAG CTCCTGGTCC AGCCTTGATC AGAAAAGGTT CTGCCGGCCT 
CAGACAAACT CTTCAGGCTC CCAGAAAACC CCATTCGCTG AGGACCAGCT GCATACAGTC 
CCAGAGAGGA GTCCAGAGAA CAGCCCCCCA GTGAAATCTA AGCATAATTA TACCCAGAAG 
GCGCAGCCTG GCCAACCTCT GCTGCCCACT GGCATCTATC CGGTACCTTC TCCGGAGCCT 
CACTTTGCCC AGGTGCCCCA GCCCTCTGTG AGTAGTAATG GCACCGTCTA CCCTGCGCTA 
GTCAAAGAGA GTGGATACAC AGCTGCTCAG GGAACCTGTA ACAAGATGGC TACCTTAGAT 
GAGAACGGGA ATCAAAATGA AGCTAGCAGG CCTGGGTTTG CCTTCTGTCA GCCCCTAGAA 
CACAACTCGG TGACTCCGGT GGAGAAAAGG CCAGAACCCA CGGCCAAATA CATCTACAAA 
GTCCATTTTT CTTCAGTGCC TGAAAATGAA GACTCCTCCC TAAAGAGGCA CATCACACCT 
CCCCACGGCC ATAGTCCATA TCCCAGCGAG AGAAAGAACA TCCACGGTGG CAGCAGAGCG 
TGTTCCAATC ACCACAGTCT CTCGTCCCCC CAGGCCCAGG CCCTGCACGT GGGTGATGAC 
AGGAGGCCTT CCAGGCTCTC TCAGCCCTGG GAGGGTGACT TTCAGGAAGA TCACAATGCC 
AACCTCAGGC AGAAAGTGGA GAGGGAGGGC CAAGGCCAGG GCCTGTCGGG CAACTCTGGC 
AGGACCAGAT CGGCCTTCTC ATCTCTGCAG AATATTCCAG AGAGTCTGAG AAGACAAAGC 
AACGTGGAGC TTGGAGAAGC CCAGGAGGTT CACCCCGGGG GCAGGTCCAA GGTGGAGGAC 
CCTGGAAGGA AAGCAGGGGC TTCAGACATC AGGGGCTACC TGGACAGATC TGTTTCCTAT 
CCGAGGCCCG AGGGGAAAAT GAACGCCGTA GACTCCGTCC ACAGTGCAGA CTCCAGGTAT 
GAGGAGTCCC CGGCCCCAGC GCTGCCACAG ACATCCGGTG CGAGCCAGCG GCGGCTCAGC 
TCCAGCAGCT CCGCCGCCCC GCAGTACAGG AAGCCCCATT GCTCCGTGCT GGAGAAGGTT 
TCTAGAATAG AGGAACGAGA GCAGGGGCGC CACAGACCCC TGAGCGTGGG CAGCTCTGCA 
TACGGCCCCG GCTACAGGCC TGGGAGGACC GGCCCGACCC CCAGTACTTC CAGCAGTGAC 
CTCGACGATC CAAAAGCCGG CAGCGTCCAT TTCTCGGAGT CCACTGAACA CCTGCGCAAT 
GGGGAGCAGA ACCCCCCAAA CGGGGAGGCT AAGCAGGAAG AGGCTTCCCG GCCCCAGTGC 
AGCCATTTGA TCCGGAGGGC CCCTGCGGAC GGCCGTGGAC CTCCTGCCCG AGGCGGCGAG 
CCCTCAAGGC CGGAGGCCCG CCTGCTCCGC AGCCAGAGCA CCTTTCAGCT CTACAGTGAG 
GCGGAGAGGG AAGCCTCGTG GTCAGAGGAC AGGCCCGGTA CCCCTGAGTC GCCCCTGCTC 
GATGCGCCTT TCAGCCGCGC CTACAGAAAC AGCATCAAGG ATGCCCAGTC CCGCGTCCTG 
GGGGCCACGT CGTTTCGTCG CCGAGACCTG GAACCGGGCA CCCCCGCCAC CTCGAGGCCC 
TGGCGCCCGC GACCCGCCTC CGCCCACGTG GGGATGCGGA GCCCCGAGGC CGCGGTGCCC 
TCGTCCTCCC CGCACACCCC TCGGGAGCGA CACAGCGTGA CCCCGGCCGC GCCCCAGGCT 
GCCCGCCGAG GCCCACGCCG GCGCCTCACT GTCGAGCAGA AGAAGCGCTC CTACTCGGAG 
CCCGAGAAGA TGAACGAGGT AGGGGTCTCG GAGGAGGCCG AGCCCACGCC CTGCGGCCCG 
CCGAGGCCCG CGCAGCCGCG CTTCTCGGAG AGCACCGTGG CCGACCGGCG CCGCATCTTC 
GAGCGCGACG GCAAGGCCTG CTCCACGCTC AGCCTGTCGG GCCCCGAGCT CAAGCAGTTC 
CAGCAGAGCG CCCTGGCCGA CTACATCCAG CGTAAGACGG GCAAGCGGCC CACAGGAGCC 
GCCTGCACAC CGGAGGCCGG GCTGCGCGAG CGCGCACAGA GCGCCTACCT CCAGGCCGGC 
CCCGCGGCGC CCGATGGCCC CGGCCTGGCC TCTGCCTGTA GCCTGAGCTC CCTGCGAGAG 
CCCGAGGCGC TGCCCCGCAA GGAGCACACT CATCCGTCGG CCGCGGACGG CCCGCAGGCG 
CCCCGTGATC GCAGCAGCTC CTTCGCCAGC GGACGCCTAG TCGGGGAGCG ACGTCGCTGG 
GACCCTCAGG TCCCCAGGCA GCTGCTCAGC GGAGCGAACT GCGAACCGAG GGGCGTCCAG 
AGGATGGACG GGGCCCCGGG CGGGCCGCCT TCCTGGGGCA TGGTGGCCGG GAAGGCCGGG 
AAGTCCAAGT CAGCCGAGGA TCTGCTGGAG CGCTCCGACA CACTGGCGGT CCCTGTGCAT 
GTGAGGTCCA GGTCGTCTCC CACCTCCGAC AAGAAAGGTC AGGATGTGCT CCTGAGGGAA 
GGCAGCAACT TTGGTTTTGT AAAGGATCCA TGCTGTTTGG CTGGCCCTGG ACCTAGGTCT 
CTCAGCTGCT CAGACAAAGG CCAGAATGAG CTGGCATTGC CCCTGCACCA CCCTACGCCC 
TGCTGGAACG GCTCAGGCTG CAAAGCCACT GTGGCTTCCT CAGCTCCTCC CGAATCTTCT 
GGAGCCGCGG ATCATCTGAA GCAACGTAGA GCGCCTGGTC CTAGGCCACT CTCAGCAGGA 
ATGCACGGCC ACTTCCCAGA CGCCAGAGCT GCCTCACTCA GCTCCCCTCT GCCATCACCT 
GTCCCCTCCA GTTACCGGTC ACAGCTTGCC ATGGATCAGC AGACTGGGCA ACAGCCTCCC 
TCATCCCCTG CCTCTGCAGT GACCCAGCCT ACCAGTCCAA GAAGCCCTGA GCTCAGCAGC 
CCAGCATATG GGCTAGGGGA GGGGATGTGG AAGAGGACAT CCCTGCCTCA GCGACCCCCT 
CCCCCTTGGG TGAAATGGGC CCACGCGGTC AGAGAGGACG GCCTTGCTGA GGACACCTTG 
GCGCCTGAGT TTGCGAACCT GAAGCACTAT AGAAACCAGC CGAGTCGTCC GAGTTCCTGC 
AGCACTTCTG ACCCAGACAC TCCAGGAAGG ATCTCGCTCC GAATATCGGA GTCAGCCCTT 
CAACCTTCCC CACCGCCCCG GGGGGACTAT GATGACGAAG TGTTCATGAA GGACTTGCAC 
CCCAAGGTTA CTTCAAGCCC CACATTTGAA GCTCTTCCCC CACCCCCGCC ACCTTCGCCA 
CCGAGCGAGG AACCTCTAGT GAATGGCACA GATGACTTTC CTCCGCCTCC TCCTCCCCAA 
GCCCTGTGTG AGGTACTGCT GGATGGTGAG GCATCCACGG AAGCTGGCAG CGGCCCCTGC 
AGAATTCCCA GGGTGATGGT CACAAGGGAA GGGCACGTGC CTGGGGCAGC CCATTCCGAA 
GGTAGTCAGA TAATGACTGC CACACCACCC CAAACCTCGG CCAAAGGTTC AGAAGCCGAG 
TCCAACACGC CTTCCAGCGC GAGCGCCCAG CCTCAGCTCA ACGGTTCTCC TGGCAAGCAG 
CTCTGTCCCA GCCAGACCCG AAACCTCACT TATGAACCTG TCGAGAGAAC TCAGGATCTA 
GGAAAGAAAA CTCACGCCGA GCCTCAGAAG ACCTCAGAAG ATATCCGGAC AGAGGCTCTA 
GCCAAGGAAA TTGTCCACCA AGACAAGTCT CTGGCAGACA TTTTAGATCC TGACTCCAGG 
ATGAAGACAA CGATGGACCT GATGGAGGGT TTATTCCCTG GAGACGCCAG TGTGCTGATG 
GATAGTGGCG CAAAGAGGAA AGCCTTAGAC ATCACCGCCA GGCGTGCGGG ATGTGAAGCC 
AAGGCGAGCG ACCACAAGGA GGCAGTGAGT GTGCTGGTCA ACTGTCCTGC CTACTACAGT 
GTCTCTGCTG CCAAGGCGGA GCTGCTCAAC AAAATCAAAG ACATGCCGGA GGAGCTGCAG 
GAGGAGGAGG GGCAGGAGGA TGTCAATGAG AAAAAGGCTG AGCTTATCGG AAGCCTTACC 
CACAAGCTGG AGAGCCTCCA GGAAGCCAAG GGGAGCCTGC TCACGGACAT CAAGCTGAAC 
AATGCCCTGG GAGAGGAGGT GGAGGCTCTG ATCAGTGAGC TCTGCAAACC CAACGAGTTT 
GACAAGTACA AGATGTTCAT AGGGGACCTG GACAAGGTGG TCAACCTGCT GCTGTCCCTG 
TCTGGACGCC TGGCCCGCGT GGAGAACGTC CTCAGAGGCC TTGGTGAAGA CGCCAGCAAA 
GAGGAAAGGA GTTCTCTGAA CGAGAAAAGG AAGGTTCTGG CTGGCCAGCA TGAGGATGCA 
CGGGAGCTCA AGGAGAACCT GGACCGGAGG GAGCGCGTGG TGCTGGATAT CCTGGCCAAC 
TACCTGTCCG CCGAGCAGCT GCAGGACTAC CAGCACTTCG TGAAGATGAA GTCCACACTC 
CTCATTGAGC AGCGGAAGCT GGATGACAAG ATAAAGCTGG GCCAGGAGCA GGTCAGGTGC 
CTGCTGGAGT CGCTTCCCTC GGACTTCAGG CCCAAGGCAG GGGCCATCTC CCTCCCTCCA 
GCCCTCACCG GCCATGGGAC TCCTGGGGGG ACATCTGTAT TCGGTGGTGT TTTCCCAACG 
TTGACCTCTC CTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Shroom3 ( XM_006534966.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006535029.1 Mus musculus shroom family member 3 (Shroom3), transcript variant X1, mRNA.

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CloneID	OMu35489
Clone ID Related Accession (Same CDS sequence)	XM_006534966.3 , XM_006534966.4
Accession Version	XM_006534966.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5700bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein Shroom3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006534966.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAGGA GCAAGGGCTG CCTGGCTCTC GTGCTTCCCA GATTTGGGAC CCAAAACTCA 
AGAAGGCAAC AGAAGCTAGA CGTGTGTGCG GCCCCGGGCC ATGCTGATCC TGGTACCTCT 
AAGTCCCTCA GCTCAGAGCT GCTCACCTGC AGTCCCCAGC ACCGGAAAGC AACATGGTCA 
GGAGGGGTGA AGCTTCGGCT AAAGCAGAGG TGCAGTGAGC CTGCCACTCG CCCTCACTCG 
TGGCATACCA CCAAATTTGG GGAGACACAG CCTGATGTCA GCATGATGCA GATATCTCAG 
GGCACAATGG GCCCTCCTTG GCACCAGAGC TACCATTCCA GCTCTTCGAC CAGTGACCTC 
TCCAACTACG ACCATGCTTA TCTGAGGCGG AGCCCTGACC AGTGCAGCTC CCAGGGGAGC 
ATGGAAAGCC TGGAGCCCAG TGGCGGATAC CCACCCTGCC ATCTCCTTTC TCCTGCCAAG 
TCCACGAGCA GCATCGACCA GCTAGGCCAC CTTCATAACA AAAGGGACTC CGCATATAGC 
TCCTTCTCCA CCAGTTCGAG CATCTTCGAG TACCCACCCC CTGGTGGCTC TGCCCGAGAA 
CGTTCGGGTT CCATGGACGT GATTTCTGCC CGGGGTGGCC TCCTAGAAGG GATGAGGCAG 
GCAGACATCC GCTACGTCAA GACGGTCTAT GATACCCGGA GAGGAGTATC TTCAGAGTAT 
GAGGTGAACC CTTCAGCCTT GCTCCTTCAA GGTAGGGATG CCCATGCCTC AGCCGACAGT 
CAGGGCTGTG CTAAATGGCA CAGCATTCCT CGGGGCAAGG GAACGCCTTC CCCGTCTTGG 
AGCCAGCAGT GTTCTGGTTC CCTGGAGACT GCTACTGACA ACCTGCCCCA GAAGGCAGGC 
GCACCCCTGC CCCCCACCCG CAGTGATAGC TATGCAGCCT TCCGCCATCG CGAGCGTCCC 
AGCTCCTGGT CCAGCCTTGA TCAGAAAAGG TTCTGCCGGC CTCAGACAAA CTCTTCAGGC 
TCCCAGAAAA CCCCATTCGC TGAGGACCAG CTGCATACAG TCCCAGAGAG GAGTCCAGAG 
AACAGCCCCC CAGTGAAATC TAAGCATAAT TATACCCAGA AGGCGCAGCC TGGCCAACCT 
CTGCTGCCCA CTGGCATCTA TCCGGTACCT TCTCCGGAGC CTCACTTTGC CCAGGTGCCC 
CAGCCCTCTG TGAGTAGTAA TGGCACCGTC TACCCTGCGC TAGTCAAAGA GAGTGGATAC 
ACAGCTGCTC AGGGAACCTG TAACAAGATG GCTACCTTAG ATGAGAACGG GAATCAAAAT 
GAAGCTAGCA GGCCTGGGTT TGCCTTCTGT CAGCCCCTAG AACACAACTC GGTGACTCCG 
GTGGAGAAAA GGCCAGAACC CACGGCCAAA TACATCTACA AAGTCCATTT TTCTTCAGTG 
CCTGAAAATG AAGACTCCTC CCTAAAGAGG CACATCACAC CTCCCCACGG CCATAGTCCA 
TATCCCAGCG AGAGAAAGAA CATCCACGGT GGCAGCAGAG CGTGTTCCAA TCACCACAGT 
CTCTCGTCCC CCCAGGCCCA GGCCCTGCAC GTGGGTGATG ACAGGAGGCC TTCCAGGCTC 
TCTCAGCCCT GGGAGGGTGA CTTTCAGGAA GATCACAATG CCAACCTCAG GCAGAAAGTG 
GAGAGGGAGG GCCAAGGCCA GGGCCTGTCG GGCAACTCTG GCAGGACCAG ATCGGCCTTC 
TCATCTCTGC AGAATATTCC AGAGAGTCTG AGAAGACAAA GCAACGTGGA GCTTGGAGAA 
GCCCAGGAGG TTCACCCCGG GGGCAGGTCC AAGGTGGAGG ACCCTGGAAG GAAAGCAGGG 
GCTTCAGACA TCAGGGGCTA CCTGGACAGA TCTGTTTCCT ATCCGAGGCC CGAGGGGAAA 
ATGAACGCCG TAGACTCCGT CCACAGTGCA GACTCCAGGT ATGAGGAGTC CCCGGCCCCA 
GCGCTGCCAC AGACATCCGG TGCGAGCCAG CGGCGGCTCA GCTCCAGCAG CTCCGCCGCC 
CCGCAGTACA GGAAGCCCCA TTGCTCCGTG CTGGAGAAGG TTTCTAGAAT AGAGGAACGA 
GAGCAGGGGC GCCACAGACC CCTGAGCGTG GGCAGCTCTG CATACGGCCC CGGCTACAGG 
CCTGGGAGGA CCGGCCCGAC CCCCAGTACT TCCAGCAGTG ACCTCGACGA TCCAAAAGCC 
GGCAGCGTCC ATTTCTCGGA GTCCACTGAA CACCTGCGCA ATGGGGAGCA GAACCCCCCA 
AACGGGGAGG CTAAGCAGGA AGAGGCTTCC CGGCCCCAGT GCAGCCATTT GATCCGGAGG 
GCCCCTGCGG ACGGCCGTGG ACCTCCTGCC CGAGGCGGCG AGCCCTCAAG GCCGGAGGCC 
CGCCTGCTCC GCAGCCAGAG CACCTTTCAG CTCTACAGTG AGGCGGAGAG GGAAGCCTCG 
TGGTCAGAGG ACAGGCCCGG TACCCCTGAG TCGCCCCTGC TCGATGCGCC TTTCAGCCGC 
GCCTACAGAA ACAGCATCAA GGATGCCCAG TCCCGCGTCC TGGGGGCCAC GTCGTTTCGT 
CGCCGAGACC TGGAACCGGG CACCCCCGCC ACCTCGAGGC CCTGGCGCCC GCGACCCGCC 
TCCGCCCACG TGGGGATGCG GAGCCCCGAG GCCGCGGTGC CCTCGTCCTC CCCGCACACC 
CCTCGGGAGC GACACAGCGT GACCCCGGCC GCGCCCCAGG CTGCCCGCCG AGGCCCACGC 
CGGCGCCTCA CTGTCGAGCA GAAGAAGCGC TCCTACTCGG AGCCCGAGAA GATGAACGAG 
GTAGGGGTCT CGGAGGAGGC CGAGCCCACG CCCTGCGGCC CGCCGAGGCC CGCGCAGCCG 
CGCTTCTCGG AGAGCACCGT GGCCGACCGG CGCCGCATCT TCGAGCGCGA CGGCAAGGCC 
TGCTCCACGC TCAGCCTGTC GGGCCCCGAG CTCAAGCAGT TCCAGCAGAG CGCCCTGGCC 
GACTACATCC AGCGTAAGAC GGGCAAGCGG CCCACAGGAG CCGCCTGCAC ACCGGAGGCC 
GGGCTGCGCG AGCGCGCACA GAGCGCCTAC CTCCAGGCCG GCCCCGCGGC GCCCGATGGC 
CCCGGCCTGG CCTCTGCCTG TAGCCTGAGC TCCCTGCGAG AGCCCGAGGC GCTGCCCCGC 
AAGGAGCACA CTCATCCGTC GGCCGCGGAC GGCCCGCAGG CGCCCCGTGA TCGCAGCAGC 
TCCTTCGCCA GCGGACGCCT AGTCGGGGAG CGACGTCGCT GGGACCCTCA GGTCCCCAGG 
CAGCTGCTCA GCGGAGCGAA CTGCGAACCG AGGGGCGTCC AGAGGATGGA CGGGGCCCCG 
GGCGGGCCGC CTTCCTGGGG CATGGTGGCC GGGAAGGCCG GGAAGTCCAA GTCAGCCGAG 
GATCTGCTGG AGCGCTCCGA CACACTGGCG GTCCCTGTGC ATGTGAGGTC CAGGTCGTCT 
CCCACCTCCG ACAAGAAAGG TCAGGATGTG CTCCTGAGGG AAGGCAGCAA CTTTGGTTTT 
GTAAAGGATC CATGCTGTTT GGCTGGCCCT GGACCTAGGT CTCTCAGCTG CTCAGACAAA 
GGCCAGAATG AGCTGGCATT GCCCCTGCAC CACCCTACGC CCTGCTGGAA CGGCTCAGGC 
TGCAAAGCCA CTGTGGCTTC CTCAGCTCCT CCCGAATCTT CTGGAGCCGC GGATCATCTG 
AAGCAACGTA GAGCGCCTGG TCCTAGGCCA CTCTCAGCAG GAATGCACGG CCACTTCCCA 
GACGCCAGAG CTGCCTCACT CAGCTCCCCT CTGCCATCAC CTGTCCCCTC CAGTTACCGG 
TCACAGCTTG CCATGGATCA GCAGACTGGG CAACAGCCTC CCTCATCCCC TGCCTCTGCA 
GTGACCCAGC CTACCAGTCC AAGAAGCCCT GAGCTCAGCA GCCCAGCATA TGGGCTAGGG 
GAGGGGATGT GGAAGAGGAC ATCCCTGCCT CAGCGACCCC CTCCCCCTTG GGTGAAATGG 
GCCCACGCGG TCAGAGAGGA CGGCCTTGCT GAGGACACCT TGGCGCCTGA GTTTGCGAAC 
CTGAAGCACT ATAGAAACCA GCCGAGTCGT CCGAGTTCCT GCAGCACTTC TGACCCAGAC 
ACTCCAGGAA GGATCTCGCT CCGAATATCG GAGTCAGCCC TTCAACCTTC CCCACCGCCC 
CGGGGGGACT ATGATGACGA AGTGTTCATG AAGGACTTGC ACCCCAAGGT TACTTCAAGC 
CCCACATTTG AAGCTCTTCC CCCACCCCCG CCACCTTCGC CACCGAGCGA GGAACCTCTA 
GTGAATGGCA CAGATGACTT TCCTCCGCCT CCTCCTCCCC AAGCCCTGTG TGAGGTACTG 
CTGGATGGTG AGGCATCCAC GGAAGCTGGC AGCGGCCCCT GCAGAATTCC CAGGGTGATG 
GTCACAAGGG AAGGGCACGT GCCTGGGGCA GCCCATTCCG AAGGTAGTCA GATAATGACT 
GCCACACCAC CCCAAACCTC GGCCAAAGGT TCAGAAGCCG AGTCCAACAC GCCTTCCAGC 
GCGAGCGCCC AGCCTCAGCT CAACGGTTCT CCTGGCAAGC AGCTCTGTCC CAGCCAGACC 
CGAAACCTCA CTTATGAACC TGTCGAGAGA ACTCAGGATC TAGGAAAGAA AACTCACGCC 
GAGCCTCAGA AGACCTCAGA AGATATCCGG ACAGAGGCTC TAGCCAAGGA AATTGTCCAC 
CAAGACAAGT CTCTGGCAGA CATTTTAGAT CCTGACTCCA GGATGAAGAC AACGATGGAC 
CTGATGGAGG GTTTATTCCC TGGAGACGCC AGTGTGCTGA TGGATAGTGG CGCAAAGAGG 
AAAGCCTTAG ACATCACCGC CAGGCGTGCG GGATGTGAAG CCAAGGCGAG CGACCACAAG 
GAGGCAGTGA GTGTGCTGGT CAACTGTCCT GCCTACTACA GTGTCTCTGC TGCCAAGGCG 
GAGCTGCTCA ACAAAATCAA AGACATGCCG GAGGAGCTGC AGGAGGAGGA GGGGCAGGAG 
GATGTCAATG AGAAAAAGGC TGAGCTTATC GGAAGCCTTA CCCACAAGCT GGAGAGCCTC 
CAGGAAGCCA AGGGGAGCCT GCTCACGGAC ATCAAGCTGA ACAATGCCCT GGGAGAGGAG 
GTGGAGGCTC TGATCAGTGA GCTCTGCAAA CCCAACGAGT TTGACAAGTA CAAGATGTTC 
ATAGGGGACC TGGACAAGGT GGTCAACCTG CTGCTGTCCC TGTCTGGACG CCTGGCCCGC 
GTGGAGAACG TCCTCAGAGG CCTTGGTGAA GACGCCAGCA AAGAGGAAAG GAGTTCTCTG 
AACGAGAAAA GGAAGGTTCT GGCTGGCCAG CATGAGGATG CACGGGAGCT CAAGGAGAAC 
CTGGACCGGA GGGAGCGCGT GGTGCTGGAT ATCCTGGCCA ACTACCTGTC CGCCGAGCAG 
CTGCAGGACT ACCAGCACTT CGTGAAGATG AAGTCCACAC TCCTCATTGA GCAGCGGAAG 
CTGGATGACA AGATAAAGCT GGGCCAGGAG CAGGTCAGGT GCCTGCTGGA GTCGCTTCCC 
TCGGACTTCA GGCCCAAGGC AGGGGCCATC TCCCTCCCTC CAGCCCTCAC CGGCCATGGG 
ACTCCTGGGG GGACATCTGT ATTCGGTGGT GTTTTCCCAA CGTTGACCTC TCCTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006535029.1
CDS	414..6113
Translation	MVRSKGCLALVLPRFGTQNSRRQQKLDVCAAPGHADPGTSKSLSSELLTCSPQHRKATWSGGVKLRLKQRCSEPATRPHSWHTTKFGETQPDVSMMQISQGTMGPPWHQSYHSSSSTSDLSNYDHAYLRRSPDQCSSQGSMESLEPSGGYPPCHLLSPAKSTSSIDQLGHLHNKRDSAYSSFSTSSSIFEYPPPGGSARERSGSMDVISARGGLLEGMRQADIRYVKTVYDTRRGVSSEYEVNPSALLLQGRDAHASADSQGCAKWHSIPRGKGTPSPSWSQQCSGSLETATDNLPQKAGAPLPPTRSDSYAAFRHRERPSSWSSLDQKRFCRPQTNSSGSQKTPFAEDQLHTVPERSPENSPPVKSKHNYTQKAQPGQPLLPTGIYPVPSPEPHFAQVPQPSVSSNGTVYPALVKESGYTAAQGTCNKMATLDENGNQNEASRPGFAFCQPLEHNSVTPVEKRPEPTAKYIYKVHFSSVPENEDSSLKRHITPPHGHSPYPSERKNIHGGSRACSNHHSLSSPQAQALHVGDDRRPSRLSQPWEGDFQEDHNANLRQKVEREGQGQGLSGNSGRTRSAFSSLQNIPESLRRQSNVELGEAQEVHPGGRSKVEDPGRKAGASDIRGYLDRSVSYPRPEGKMNAVDSVHSADSRYEESPAPALPQTSGASQRRLSSSSSAAPQYRKPHCSVLEKVSRIEEREQGRHRPLSVGSSAYGPGYRPGRTGPTPSTSSSDLDDPKAGSVHFSESTEHLRNGEQNPPNGEAKQEEASRPQCSHLIRRAPADGRGPPARGGEPSRPEARLLRSQSTFQLYSEAEREASWSEDRPGTPESPLLDAPFSRAYRNSIKDAQSRVLGATSFRRRDLEPGTPATSRPWRPRPASAHVGMRSPEAAVPSSSPHTPRERHSVTPAAPQAARRGPRRRLTVEQKKRSYSEPEKMNEVGVSEEAEPTPCGPPRPAQPRFSESTVADRRRIFERDGKACSTLSLSGPELKQFQQSALADYIQRKTGKRPTGAACTPEAGLRERAQSAYLQAGPAAPDGPGLASACSLSSLREPEALPRKEHTHPSAADGPQAPRDRSSSFASGRLVGERRRWDPQVPRQLLSGANCEPRGVQRMDGAPGGPPSWGMVAGKAGKSKSAEDLLERSDTLAVPVHVRSRSSPTSDKKGQDVLLREGSNFGFVKDPCCLAGPGPRSLSCSDKGQNELALPLHHPTPCWNGSGCKATVASSAPPESSGAADHLKQRRAPGPRPLSAGMHGHFPDARAASLSSPLPSPVPSSYRSQLAMDQQTGQQPPSSPASAVTQPTSPRSPELSSPAYGLGEGMWKRTSLPQRPPPPWVKWAHAVREDGLAEDTLAPEFANLKHYRNQPSRPSSCSTSDPDTPGRISLRISESALQPSPPPRGDYDDEVFMKDLHPKVTSSPTFEALPPPPPPSPPSEEPLVNGTDDFPPPPPPQALCEVLLDGEASTEAGSGPCRIPRVMVTREGHVPGAAHSEGSQIMTATPPQTSAKGSEAESNTPSSASAQPQLNGSPGKQLCPSQTRNLTYEPVERTQDLGKKTHAEPQKTSEDIRTEALAKEIVHQDKSLADILDPDSRMKTTMDLMEGLFPGDASVLMDSGAKRKALDITARRAGCEAKASDHKEAVSVLVNCPAYYSVSAAKAELLNKIKDMPEELQEEEGQEDVNEKKAELIGSLTHKLESLQEAKGSLLTDIKLNNALGEEVEALISELCKPNEFDKYKMFIGDLDKVVNLLLSLSGRLARVENVLRGLGEDASKEERSSLNEKRKVLAGQHEDARELKENLDRRERVVLDILANYLSAEQLQDYQHFVKMKSTLLIEQRKLDDKIKLGQEQVRCLLESLPSDFRPKAGAISLPPALTGHGTPGGTSVFGGVFPTLTSPL

Target ORF information:

RefSeq Version	XM_006534966.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus shroom family member 3 (Shroom3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006534966.4

ORF Insert Sequence:

ATGGTGAGGA GCAAGGGCTG CCTGGCTCTC GTGCTTCCCA GATTTGGGAC CCAAAACTCA 
AGAAGGCAAC AGAAGCTAGA CGTGTGTGCG GCCCCGGGCC ATGCTGATCC TGGTACCTCT 
AAGTCCCTCA GCTCAGAGCT GCTCACCTGC AGTCCCCAGC ACCGGAAAGC AACATGGTCA 
GGAGGGGTGA AGCTTCGGCT AAAGCAGAGG TGCAGTGAGC CTGCCACTCG CCCTCACTCG 
TGGCATACCA CCAAATTTGG GGAGACACAG CCTGATGTCA GCATGATGCA GATATCTCAG 
GGCACAATGG GCCCTCCTTG GCACCAGAGC TACCATTCCA GCTCTTCGAC CAGTGACCTC 
TCCAACTACG ACCATGCTTA TCTGAGGCGG AGCCCTGACC AGTGCAGCTC CCAGGGGAGC 
ATGGAAAGCC TGGAGCCCAG TGGCGGATAC CCACCCTGCC ATCTCCTTTC TCCTGCCAAG 
TCCACGAGCA GCATCGACCA GCTAGGCCAC CTTCATAACA AAAGGGACTC CGCATATAGC 
TCCTTCTCCA CCAGTTCGAG CATCTTCGAG TACCCACCCC CTGGTGGCTC TGCCCGAGAA 
CGTTCGGGTT CCATGGACGT GATTTCTGCC CGGGGTGGCC TCCTAGAAGG GATGAGGCAG 
GCAGACATCC GCTACGTCAA GACGGTCTAT GATACCCGGA GAGGAGTATC TTCAGAGTAT 
GAGGTGAACC CTTCAGCCTT GCTCCTTCAA GGTAGGGATG CCCATGCCTC AGCCGACAGT 
CAGGGCTGTG CTAAATGGCA CAGCATTCCT CGGGGCAAGG GAACGCCTTC CCCGTCTTGG 
AGCCAGCAGT GTTCTGGTTC CCTGGAGACT GCTACTGACA ACCTGCCCCA GAAGGCAGGC 
GCACCCCTGC CCCCCACCCG CAGTGATAGC TATGCAGCCT TCCGCCATCG CGAGCGTCCC 
AGCTCCTGGT CCAGCCTTGA TCAGAAAAGG TTCTGCCGGC CTCAGACAAA CTCTTCAGGC 
TCCCAGAAAA CCCCATTCGC TGAGGACCAG CTGCATACAG TCCCAGAGAG GAGTCCAGAG 
AACAGCCCCC CAGTGAAATC TAAGCATAAT TATACCCAGA AGGCGCAGCC TGGCCAACCT 
CTGCTGCCCA CTGGCATCTA TCCGGTACCT TCTCCGGAGC CTCACTTTGC CCAGGTGCCC 
CAGCCCTCTG TGAGTAGTAA TGGCACCGTC TACCCTGCGC TAGTCAAAGA GAGTGGATAC 
ACAGCTGCTC AGGGAACCTG TAACAAGATG GCTACCTTAG ATGAGAACGG GAATCAAAAT 
GAAGCTAGCA GGCCTGGGTT TGCCTTCTGT CAGCCCCTAG AACACAACTC GGTGACTCCG 
GTGGAGAAAA GGCCAGAACC CACGGCCAAA TACATCTACA AAGTCCATTT TTCTTCAGTG 
CCTGAAAATG AAGACTCCTC CCTAAAGAGG CACATCACAC CTCCCCACGG CCATAGTCCA 
TATCCCAGCG AGAGAAAGAA CATCCACGGT GGCAGCAGAG CGTGTTCCAA TCACCACAGT 
CTCTCGTCCC CCCAGGCCCA GGCCCTGCAC GTGGGTGATG ACAGGAGGCC TTCCAGGCTC 
TCTCAGCCCT GGGAGGGTGA CTTTCAGGAA GATCACAATG CCAACCTCAG GCAGAAAGTG 
GAGAGGGAGG GCCAAGGCCA GGGCCTGTCG GGCAACTCTG GCAGGACCAG ATCGGCCTTC 
TCATCTCTGC AGAATATTCC AGAGAGTCTG AGAAGACAAA GCAACGTGGA GCTTGGAGAA 
GCCCAGGAGG TTCACCCCGG GGGCAGGTCC AAGGTGGAGG ACCCTGGAAG GAAAGCAGGG 
GCTTCAGACA TCAGGGGCTA CCTGGACAGA TCTGTTTCCT ATCCGAGGCC CGAGGGGAAA 
ATGAACGCCG TAGACTCCGT CCACAGTGCA GACTCCAGGT ATGAGGAGTC CCCGGCCCCA 
GCGCTGCCAC AGACATCCGG TGCGAGCCAG CGGCGGCTCA GCTCCAGCAG CTCCGCCGCC 
CCGCAGTACA GGAAGCCCCA TTGCTCCGTG CTGGAGAAGG TTTCTAGAAT AGAGGAACGA 
GAGCAGGGGC GCCACAGACC CCTGAGCGTG GGCAGCTCTG CATACGGCCC CGGCTACAGG 
CCTGGGAGGA CCGGCCCGAC CCCCAGTACT TCCAGCAGTG ACCTCGACGA TCCAAAAGCC 
GGCAGCGTCC ATTTCTCGGA GTCCACTGAA CACCTGCGCA ATGGGGAGCA GAACCCCCCA 
AACGGGGAGG CTAAGCAGGA AGAGGCTTCC CGGCCCCAGT GCAGCCATTT GATCCGGAGG 
GCCCCTGCGG ACGGCCGTGG ACCTCCTGCC CGAGGCGGCG AGCCCTCAAG GCCGGAGGCC 
CGCCTGCTCC GCAGCCAGAG CACCTTTCAG CTCTACAGTG AGGCGGAGAG GGAAGCCTCG 
TGGTCAGAGG ACAGGCCCGG TACCCCTGAG TCGCCCCTGC TCGATGCGCC TTTCAGCCGC 
GCCTACAGAA ACAGCATCAA GGATGCCCAG TCCCGCGTCC TGGGGGCCAC GTCGTTTCGT 
CGCCGAGACC TGGAACCGGG CACCCCCGCC ACCTCGAGGC CCTGGCGCCC GCGACCCGCC 
TCCGCCCACG TGGGGATGCG GAGCCCCGAG GCCGCGGTGC CCTCGTCCTC CCCGCACACC 
CCTCGGGAGC GACACAGCGT GACCCCGGCC GCGCCCCAGG CTGCCCGCCG AGGCCCACGC 
CGGCGCCTCA CTGTCGAGCA GAAGAAGCGC TCCTACTCGG AGCCCGAGAA GATGAACGAG 
GTAGGGGTCT CGGAGGAGGC CGAGCCCACG CCCTGCGGCC CGCCGAGGCC CGCGCAGCCG 
CGCTTCTCGG AGAGCACCGT GGCCGACCGG CGCCGCATCT TCGAGCGCGA CGGCAAGGCC 
TGCTCCACGC TCAGCCTGTC GGGCCCCGAG CTCAAGCAGT TCCAGCAGAG CGCCCTGGCC 
GACTACATCC AGCGTAAGAC GGGCAAGCGG CCCACAGGAG CCGCCTGCAC ACCGGAGGCC 
GGGCTGCGCG AGCGCGCACA GAGCGCCTAC CTCCAGGCCG GCCCCGCGGC GCCCGATGGC 
CCCGGCCTGG CCTCTGCCTG TAGCCTGAGC TCCCTGCGAG AGCCCGAGGC GCTGCCCCGC 
AAGGAGCACA CTCATCCGTC GGCCGCGGAC GGCCCGCAGG CGCCCCGTGA TCGCAGCAGC 
TCCTTCGCCA GCGGACGCCT AGTCGGGGAG CGACGTCGCT GGGACCCTCA GGTCCCCAGG 
CAGCTGCTCA GCGGAGCGAA CTGCGAACCG AGGGGCGTCC AGAGGATGGA CGGGGCCCCG 
GGCGGGCCGC CTTCCTGGGG CATGGTGGCC GGGAAGGCCG GGAAGTCCAA GTCAGCCGAG 
GATCTGCTGG AGCGCTCCGA CACACTGGCG GTCCCTGTGC ATGTGAGGTC CAGGTCGTCT 
CCCACCTCCG ACAAGAAAGG TCAGGATGTG CTCCTGAGGG AAGGCAGCAA CTTTGGTTTT 
GTAAAGGATC CATGCTGTTT GGCTGGCCCT GGACCTAGGT CTCTCAGCTG CTCAGACAAA 
GGCCAGAATG AGCTGGCATT GCCCCTGCAC CACCCTACGC CCTGCTGGAA CGGCTCAGGC 
TGCAAAGCCA CTGTGGCTTC CTCAGCTCCT CCCGAATCTT CTGGAGCCGC GGATCATCTG 
AAGCAACGTA GAGCGCCTGG TCCTAGGCCA CTCTCAGCAG GAATGCACGG CCACTTCCCA 
GACGCCAGAG CTGCCTCACT CAGCTCCCCT CTGCCATCAC CTGTCCCCTC CAGTTACCGG 
TCACAGCTTG CCATGGATCA GCAGACTGGG CAACAGCCTC CCTCATCCCC TGCCTCTGCA 
GTGACCCAGC CTACCAGTCC AAGAAGCCCT GAGCTCAGCA GCCCAGCATA TGGGCTAGGG 
GAGGGGATGT GGAAGAGGAC ATCCCTGCCT CAGCGACCCC CTCCCCCTTG GGTGAAATGG 
GCCCACGCGG TCAGAGAGGA CGGCCTTGCT GAGGACACCT TGGCGCCTGA GTTTGCGAAC 
CTGAAGCACT ATAGAAACCA GCCGAGTCGT CCGAGTTCCT GCAGCACTTC TGACCCAGAC 
ACTCCAGGAA GGATCTCGCT CCGAATATCG GAGTCAGCCC TTCAACCTTC CCCACCGCCC 
CGGGGGGACT ATGATGACGA AGTGTTCATG AAGGACTTGC ACCCCAAGGT TACTTCAAGC 
CCCACATTTG AAGCTCTTCC CCCACCCCCG CCACCTTCGC CACCGAGCGA GGAACCTCTA 
GTGAATGGCA CAGATGACTT TCCTCCGCCT CCTCCTCCCC AAGCCCTGTG TGAGGTACTG 
CTGGATGGTG AGGCATCCAC GGAAGCTGGC AGCGGCCCCT GCAGAATTCC CAGGGTGATG 
GTCACAAGGG AAGGGCACGT GCCTGGGGCA GCCCATTCCG AAGGTAGTCA GATAATGACT 
GCCACACCAC CCCAAACCTC GGCCAAAGGT TCAGAAGCCG AGTCCAACAC GCCTTCCAGC 
GCGAGCGCCC AGCCTCAGCT CAACGGTTCT CCTGGCAAGC AGCTCTGTCC CAGCCAGACC 
CGAAACCTCA CTTATGAACC TGTCGAGAGA ACTCAGGATC TAGGAAAGAA AACTCACGCC 
GAGCCTCAGA AGACCTCAGA AGATATCCGG ACAGAGGCTC TAGCCAAGGA AATTGTCCAC 
CAAGACAAGT CTCTGGCAGA CATTTTAGAT CCTGACTCCA GGATGAAGAC AACGATGGAC 
CTGATGGAGG GTTTATTCCC TGGAGACGCC AGTGTGCTGA TGGATAGTGG CGCAAAGAGG 
AAAGCCTTAG ACATCACCGC CAGGCGTGCG GGATGTGAAG CCAAGGCGAG CGACCACAAG 
GAGGCAGTGA GTGTGCTGGT CAACTGTCCT GCCTACTACA GTGTCTCTGC TGCCAAGGCG 
GAGCTGCTCA ACAAAATCAA AGACATGCCG GAGGAGCTGC AGGAGGAGGA GGGGCAGGAG 
GATGTCAATG AGAAAAAGGC TGAGCTTATC GGAAGCCTTA CCCACAAGCT GGAGAGCCTC 
CAGGAAGCCA AGGGGAGCCT GCTCACGGAC ATCAAGCTGA ACAATGCCCT GGGAGAGGAG 
GTGGAGGCTC TGATCAGTGA GCTCTGCAAA CCCAACGAGT TTGACAAGTA CAAGATGTTC 
ATAGGGGACC TGGACAAGGT GGTCAACCTG CTGCTGTCCC TGTCTGGACG CCTGGCCCGC 
GTGGAGAACG TCCTCAGAGG CCTTGGTGAA GACGCCAGCA AAGAGGAAAG GAGTTCTCTG 
AACGAGAAAA GGAAGGTTCT GGCTGGCCAG CATGAGGATG CACGGGAGCT CAAGGAGAAC 
CTGGACCGGA GGGAGCGCGT GGTGCTGGAT ATCCTGGCCA ACTACCTGTC CGCCGAGCAG 
CTGCAGGACT ACCAGCACTT CGTGAAGATG AAGTCCACAC TCCTCATTGA GCAGCGGAAG 
CTGGATGACA AGATAAAGCT GGGCCAGGAG CAGGTCAGGT GCCTGCTGGA GTCGCTTCCC 
TCGGACTTCA GGCCCAAGGC AGGGGCCATC TCCCTCCCTC CAGCCCTCAC CGGCCATGGG 
ACTCCTGGGG GGACATCTGT ATTCGGTGGT GTTTTCCCAA CGTTGACCTC TCCTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Shroom3 ( NM_001077595.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001071063.1 Mus musculus shroom family member 3 (Shroom3), transcript variant 2, mRNA.

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CloneID	OMu01300
Clone ID Related Accession (Same CDS sequence)	XM_006534968.2 , NM_001077595.2 , NM_001077596.2 , XM_006534968.3
Accession Version	NM_001077595.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5418bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-21
Organism	Mus musculus(house mouse)
Product	protein Shroom3 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ103792.1, AK164918.1, AK147493.1 and BM942679.1. On Oct 19, 2013 this sequence version replaced NM_001077595.1. Transcript Variant: This variant (2) differs in the 5' UTR and the 5' coding region and initiates translation at a downstream start codon, compared to variant 1. Variants 2 and 3 encode the same isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK164918.1, AF199422.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGATGCAGA TATCTCAGGG CACAATGGGC CCTCCTTGGC ACCAGAGCTA CCATTCCAGC 
TCTTCGACCA GTGACCTCTC CAACTACGAC CATGCTTATC TGAGGCGGAG CCCTGACCAG 
TGCAGCTCCC AGGGGAGCAT GGAAAGCCTG GAGCCCAGTG GCGGATACCC ACCCTGCCAT 
CTCCTTTCTC CTGCCAAGTC CACGAGCAGC ATCGACCAGC TAGGCCACCT TCATAACAAA 
AGGGACTCCG CATATAGCTC CTTCTCCACC AGTTCGAGCA TCTTCGAGTA CCCACCCCCT 
GGTGGCTCTG CCCGAGAACG TTCGGGTTCC ATGGACGTGA TTTCTGCCCG GGGTGGCCTC 
CTAGAAGGGA TGAGGCAGGC AGACATCCGC TACGTCAAGA CGGTCTATGA TACCCGGAGA 
GGAGTATCTT CAGAGTATGA GGTGAACCCT TCAGCCTTGC TCCTTCAAGG TAGGGATGCC 
CATGCCTCAG CCGACAGTCA GGGCTGTGCT AAATGGCACA GCATTCCTCG GGGCAAGGGA 
ACGCCTTCCC CGTCTTGGAG CCAGCAGTGT TCTGGTTCCC TGGAGACTGC TACTGACAAC 
CTGCCCCAGA AGGCAGGCGC ACCCCTGCCC CCCACCCGCA GTGATAGCTA TGCAGCCTTC 
CGCCATCGCG AGCGTCCCAG CTCCTGGTCC AGCCTTGATC AGAAAAGGTT CTGCCGGCCT 
CAGACAAACT CTTCAGGCTC CCAGAAAACC CCATTCGCTG AGGACCAGCT GCATACAGTC 
CCAGAGAGGA GTCCAGAGAA CAGCCCCCCA GTGAAATCTA AGCATAATTA TACCCAGAAG 
GCGCAGCCTG GCCAACCTCT GCTGCCCACT GGCATCTATC CGGTACCTTC TCCGGAGCCT 
CACTTTGCCC AGGTGCCCCA GCCCTCTGTG AGTAGTAATG GCACCGTCTA CCCTGCGCTA 
GTCAAAGAGA GTGGATACAC AGCTGCTCAG GGAACCTGTA ACAAGATGGC TACCTTAGAT 
GAGAACGGGA ATCAAAATGA AGCTAGCAGG CCTGGGTTTG CCTTCTGTCA GCCCCTAGAA 
CACAACTCGG TGACTCCGGT GGAGAAAAGG CCAGAACCCA CGGCCAAATA CATCTACAAA 
GTCCATTTTT CTTCAGTGCC TGAAAATGAA GACTCCTCCC TAAAGAGGCA CATCACACCT 
CCCCACGGCC ATAGTCCATA TCCCAGCGAG AGAAAGAACA TCCACGGTGG CAGCAGAGCG 
TGTTCCAATC ACCACAGTCT CTCGTCCCCC CAGGCCCAGG CCCTGCACGT GGGTGATGAC 
AGGAGGCCTT CCAGGCTCTC TCAGCCCTGG GAGGGTGACT TTCAGGAAGA TCACAATGCC 
AACCTCAGGC AGAAAGTGGA GAGGGAGGGC CAAGGCCAGG GCCTGTCGGG CAACTCTGGC 
AGGACCAGAT CGGCCTTCTC ATCTCTGCAG AATATTCCAG AGAGTCTGAG AAGACAAAGC 
AACGTGGAGC TTGGAGAAGC CCAGGAGGTT CACCCCGGGG GCAGGTCCAA GGTGGAGGAC 
CCTGGAAGGA AAGCAGGGGC TTCAGACATC AGGGGCTACC TGGACAGATC TGTTTCCTAT 
CCGAGGCCCG AGGGGAAAAT GAACGCCGTA GACTCCGTCC ACAGTGCAGA CTCCAGGTAT 
GAGGAGTCCC CGGCCCCAGC GCTGCCACAG ACATCCGGTG CGAGCCAGCG GCGGCTCAGC 
TCCAGCAGCT CCGCCGCCCC GCAGTACAGG AAGCCCCATT GCTCCGTGCT GGAGAAGGTT 
TCTAGAATAG AGGAACGAGA GCAGGGGCGC CACAGACCCC TGAGCGTGGG CAGCTCTGCA 
TACGGCCCCG GCTACAGGCC TGGGAGGACC GGCCCGACCC CCAGTACTTC CAGCAGTGAC 
CTCGACGATC CAAAAGCCGG CAGCGTCCAT TTCTCGGAGT CCACTGAACA CCTGCGCAAT 
GGGGAGCAGA ACCCCCCAAA CGGGGAGGCT AAGCAGGAAG AGGCTTCCCG GCCCCAGTGC 
AGCCATTTGA TCCGGAGGGC CCCTGCGGAC GGCCGTGGAC CTCCTGCCCG AGGCGGCGAG 
CCCTCAAGGC CGGAGGCCCG CCTGCTCCGC AGCCAGAGCA CCTTTCAGCT CTACAGTGAG 
GCGGAGAGGG AAGCCTCGTG GTCAGAGGAC AGGCCCGGTA CCCCTGAGTC GCCCCTGCTC 
GATGCGCCTT TCAGCCGCGC CTACAGAAAC AGCATCAAGG ATGCCCAGTC CCGCGTCCTG 
GGGGCCACGT CGTTTCGTCG CCGAGACCTG GAACCGGGCA CCCCCGCCAC CTCGAGGCCC 
TGGCGCCCGC GACCCGCCTC CGCCCACGTG GGGATGCGGA GCCCCGAGGC CGCGGTGCCC 
TCGTCCTCCC CGCACACCCC TCGGGAGCGA CACAGCGTGA CCCCGGCCGC GCCCCAGGCT 
GCCCGCCGAG GCCCACGCCG GCGCCTCACT GTCGAGCAGA AGAAGCGCTC CTACTCGGAG 
CCCGAGAAGA TGAACGAGGT AGGGGTCTCG GAGGAGGCCG AGCCCACGCC CTGCGGCCCG 
CCGAGGCCCG CGCAGCCGCG CTTCTCGGAG AGCACCGTGG CCGACCGGCG CCGCATCTTC 
GAGCGCGACG GCAAGGCCTG CTCCACGCTC AGCCTGTCGG GCCCCGAGCT CAAGCAGTTC 
CAGCAGAGCG CCCTGGCCGA CTACATCCAG CGTAAGACGG GCAAGCGGCC CACAGGAGCC 
GCCTGCACAC CGGAGGCCGG GCTGCGCGAG CGCGCACAGA GCGCCTACCT CCAGGCCGGC 
CCCGCGGCGC CCGATGGCCC CGGCCTGGCC TCTGCCTGTA GCCTGAGCTC CCTGCGAGAG 
CCCGAGGCGC TGCCCCGCAA GGAGCACACT CATCCGTCGG CCGCGGACGG CCCGCAGGCG 
CCCCGTGATC GCAGCAGCTC CTTCGCCAGC GGACGCCTAG TCGGGGAGCG ACGTCGCTGG 
GACCCTCAGG TCCCCAGGCA GCTGCTCAGC GGAGCGAACT GCGAACCGAG GGGCGTCCAG 
AGGATGGACG GGGCCCCGGG CGGGCCGCCT TCCTGGGGCA TGGTGGCCGG GAAGGCCGGG 
AAGTCCAAGT CAGCCGAGGA TCTGCTGGAG CGCTCCGACA CACTGGCGGT CCCTGTGCAT 
GTGAGGTCCA GGTCGTCTCC CACCTCCGAC AAGAAAGGTC AGGATGTGCT CCTGAGGGAA 
GGCAGCAACT TTGGTTTTGT AAAGGATCCA TGCTGTTTGG CTGGCCCTGG ACCTAGGTCT 
CTCAGCTGCT CAGACAAAGG CCAGAATGAG CTGGCATTGC CCCTGCACCA CCCTACGCCC 
TGCTGGAACG GCTCAGGCTG CAAAGCCACT GTGGCTTCCT CAGCTCCTCC CGAATCTTCT 
GGAGCCGCGG ATCATCTGAA GCAACGTAGA GCGCCTGGTC CTAGGCCACT CTCAGCAGGA 
ATGCACGGCC ACTTCCCAGA CGCCAGAGCT GCCTCACTCA GCTCCCCTCT GCCATCACCT 
GTCCCCTCCA GTTACCGGTC ACAGCTTGCC ATGGATCAGC AGACTGGGCA ACAGCCTCCC 
TCATCCCCTG CCTCTGCAGT GACCCAGCCT ACCAGTCCAA GAAGCCCTGA GCTCAGCAGC 
CCAGCATATG GGCTAGGGGA GGGGATGTGG AAGAGGACAT CCCTGCCTCA GCGACCCCCT 
CCCCCTTGGG TGAAATGGGC CCACGCGGTC AGAGAGGACG GCCTTGCTGA GGACACCTTG 
GCGCCTGAGT TTGCGAACCT GAAGCACTAT AGAAACCAGC CGAGTCGTCC GAGTTCCTGC 
AGCACTTCTG ACCCAGACAC TCCAGGAAGG ATCTCGCTCC GAATATCGGA GTCAGCCCTT 
CAACCTTCCC CACCGCCCCG GGGGGACTAT GATGACGAAG TGTTCATGAA GGACTTGCAC 
CCCAAGGTTA CTTCAAGCCC CACATTTGAA GCTCTTCCCC CACCCCCGCC ACCTTCGCCA 
CCGAGCGAGG AACCTCTAGT GAATGGCACA GATGACTTTC CTCCGCCTCC TCCTCCCCAA 
GCCCTGTGTG AGGTACTGCT GGATGGTGAG GCATCCACGG AAGCTGGCAG CGGCCCCTGC 
AGAATTCCCA GGGTGATGGT CACAAGGGAA GGGCACGTGC CTGGGGCAGC CCATTCCGAA 
GGTAGTCAGA TAATGACTGC CACACCACCC CAAACCTCGG CCAAAGGTTC AGAAGCCGAG 
TCCAACACGC CTTCCAGCGC GAGCGCCCAG CCTCAGCTCA ACGGTTCTCC TGGCAAGCAG 
CTCTGTCCCA GCCAGACCCG AAACCTCACT TATGAACCTG TCGAGAGAAC TCAGGATCTA 
GGAAAGAAAA CTCACGCCGA GCCTCAGAAG ACCTCAGAAG ATATCCGGAC AGAGGCTCTA 
GCCAAGGAAA TTGTCCACCA AGACAAGTCT CTGGCAGACA TTTTAGATCC TGACTCCAGG 
ATGAAGACAA CGATGGACCT GATGGAGGGT TTATTCCCTG GAGACGCCAG TGTGCTGATG 
GATAGTGGCG CAAAGAGGAA AGCCTTAGAC ATCACCGCCA GGCGTGCGGG ATGTGAAGCC 
AAGGCGAGCG ACCACAAGGA GGCAGTGAGT GTGCTGGTCA ACTGTCCTGC CTACTACAGT 
GTCTCTGCTG CCAAGGCGGA GCTGCTCAAC AAAATCAAAG ACATGCCGGA GGAGCTGCAG 
GAGGAGGAGG GGCAGGAGGA TGTCAATGAG AAAAAGGCTG AGCTTATCGG AAGCCTTACC 
CACAAGCTGG AGAGCCTCCA GGAAGCCAAG GGGAGCCTGC TCACGGACAT CAAGCTGAAC 
AATGCCCTGG GAGAGGAGGT GGAGGCTCTG ATCAGTGAGC TCTGCAAACC CAACGAGTTT 
GACAAGTACA AGATGTTCAT AGGGGACCTG GACAAGGTGG TCAACCTGCT GCTGTCCCTG 
TCTGGACGCC TGGCCCGCGT GGAGAACGTC CTCAGAGGCC TTGGTGAAGA CGCCAGCAAA 
GAGGAAAGGA GTTCTCTGAA CGAGAAAAGG AAGGTTCTGG CTGGCCAGCA TGAGGATGCA 
CGGGAGCTCA AGGAGAACCT GGACCGGAGG GAGCGCGTGG TGCTGGATAT CCTGGCCAAC 
TACCTGTCCG CCGAGCAGCT GCAGGACTAC CAGCACTTCG TGAAGATGAA GTCCACACTC 
CTCATTGAGC AGCGGAAGCT GGATGACAAG ATAAAGCTGG GCCAGGAGCA GGTCAGGTGC 
CTGCTGGAGT CGCTTCCCTC GGACTTCAGG CCCAAGGCAG GGGCCATCTC CCTCCCTCCA 
GCCCTCACCG GCCATGGGAC TCCTGGGGGG ACATCTGTAT TCGGTGGTGT TTTCCCAACG 
TTGACCTCTC CTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001071063.1
CDS	386..5803
Translation	MMQISQGTMGPPWHQSYHSSSSTSDLSNYDHAYLRRSPDQCSSQGSMESLEPSGGYPPCHLLSPAKSTSSIDQLGHLHNKRDSAYSSFSTSSSIFEYPPPGGSARERSGSMDVISARGGLLEGMRQADIRYVKTVYDTRRGVSSEYEVNPSALLLQGRDAHASADSQGCAKWHSIPRGKGTPSPSWSQQCSGSLETATDNLPQKAGAPLPPTRSDSYAAFRHRERPSSWSSLDQKRFCRPQTNSSGSQKTPFAEDQLHTVPERSPENSPPVKSKHNYTQKAQPGQPLLPTGIYPVPSPEPHFAQVPQPSVSSNGTVYPALVKESGYTAAQGTCNKMATLDENGNQNEASRPGFAFCQPLEHNSVTPVEKRPEPTAKYIYKVHFSSVPENEDSSLKRHITPPHGHSPYPSERKNIHGGSRACSNHHSLSSPQAQALHVGDDRRPSRLSQPWEGDFQEDHNANLRQKVEREGQGQGLSGNSGRTRSAFSSLQNIPESLRRQSNVELGEAQEVHPGGRSKVEDPGRKAGASDIRGYLDRSVSYPRPEGKMNAVDSVHSADSRYEESPAPALPQTSGASQRRLSSSSSAAPQYRKPHCSVLEKVSRIEEREQGRHRPLSVGSSAYGPGYRPGRTGPTPSTSSSDLDDPKAGSVHFSESTEHLRNGEQNPPNGEAKQEEASRPQCSHLIRRAPADGRGPPARGGEPSRPEARLLRSQSTFQLYSEAEREASWSEDRPGTPESPLLDAPFSRAYRNSIKDAQSRVLGATSFRRRDLEPGTPATSRPWRPRPASAHVGMRSPEAAVPSSSPHTPRERHSVTPAAPQAARRGPRRRLTVEQKKRSYSEPEKMNEVGVSEEAEPTPCGPPRPAQPRFSESTVADRRRIFERDGKACSTLSLSGPELKQFQQSALADYIQRKTGKRPTGAACTPEAGLRERAQSAYLQAGPAAPDGPGLASACSLSSLREPEALPRKEHTHPSAADGPQAPRDRSSSFASGRLVGERRRWDPQVPRQLLSGANCEPRGVQRMDGAPGGPPSWGMVAGKAGKSKSAEDLLERSDTLAVPVHVRSRSSPTSDKKGQDVLLREGSNFGFVKDPCCLAGPGPRSLSCSDKGQNELALPLHHPTPCWNGSGCKATVASSAPPESSGAADHLKQRRAPGPRPLSAGMHGHFPDARAASLSSPLPSPVPSSYRSQLAMDQQTGQQPPSSPASAVTQPTSPRSPELSSPAYGLGEGMWKRTSLPQRPPPPWVKWAHAVREDGLAEDTLAPEFANLKHYRNQPSRPSSCSTSDPDTPGRISLRISESALQPSPPPRGDYDDEVFMKDLHPKVTSSPTFEALPPPPPPSPPSEEPLVNGTDDFPPPPPPQALCEVLLDGEASTEAGSGPCRIPRVMVTREGHVPGAAHSEGSQIMTATPPQTSAKGSEAESNTPSSASAQPQLNGSPGKQLCPSQTRNLTYEPVERTQDLGKKTHAEPQKTSEDIRTEALAKEIVHQDKSLADILDPDSRMKTTMDLMEGLFPGDASVLMDSGAKRKALDITARRAGCEAKASDHKEAVSVLVNCPAYYSVSAAKAELLNKIKDMPEELQEEEGQEDVNEKKAELIGSLTHKLESLQEAKGSLLTDIKLNNALGEEVEALISELCKPNEFDKYKMFIGDLDKVVNLLLSLSGRLARVENVLRGLGEDASKEERSSLNEKRKVLAGQHEDARELKENLDRRERVVLDILANYLSAEQLQDYQHFVKMKSTLLIEQRKLDDKIKLGQEQVRCLLESLPSDFRPKAGAISLPPALTGHGTPGGTSVFGGVFPTLTSPL

Target ORF information:

RefSeq Version	NM_001077595.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus shroom family member 3 (Shroom3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001077595.2

ORF Insert Sequence:

ATGATGCAGA TATCTCAGGG CACAATGGGC CCTCCTTGGC ACCAGAGCTA CCATTCCAGC 
TCTTCGACCA GTGACCTCTC CAACTACGAC CATGCTTATC TGAGGCGGAG CCCTGACCAG 
TGCAGCTCCC AGGGGAGCAT GGAAAGCCTG GAGCCCAGTG GCGGATACCC ACCCTGCCAT 
CTCCTTTCTC CTGCCAAGTC CACGAGCAGC ATCGACCAGC TAGGCCACCT TCATAACAAA 
AGGGACTCCG CATATAGCTC CTTCTCCACC AGTTCGAGCA TCTTCGAGTA CCCACCCCCT 
GGTGGCTCTG CCCGAGAACG TTCGGGTTCC ATGGACGTGA TTTCTGCCCG GGGTGGCCTC 
CTAGAAGGGA TGAGGCAGGC AGACATCCGC TACGTCAAGA CGGTCTATGA TACCCGGAGA 
GGAGTATCTT CAGAGTATGA GGTGAACCCT TCAGCCTTGC TCCTTCAAGG TAGGGATGCC 
CATGCCTCAG CCGACAGTCA GGGCTGTGCT AAATGGCACA GCATTCCTCG GGGCAAGGGA 
ACGCCTTCCC CGTCTTGGAG CCAGCAGTGT TCTGGTTCCC TGGAGACTGC TACTGACAAC 
CTGCCCCAGA AGGCAGGCGC ACCCCTGCCC CCCACCCGCA GTGATAGCTA TGCAGCCTTC 
CGCCATCGCG AGCGTCCCAG CTCCTGGTCC AGCCTTGATC AGAAAAGGTT CTGCCGGCCT 
CAGACAAACT CTTCAGGCTC CCAGAAAACC CCATTCGCTG AGGACCAGCT GCATACAGTC 
CCAGAGAGGA GTCCAGAGAA CAGCCCCCCA GTGAAATCTA AGCATAATTA TACCCAGAAG 
GCGCAGCCTG GCCAACCTCT GCTGCCCACT GGCATCTATC CGGTACCTTC TCCGGAGCCT 
CACTTTGCCC AGGTGCCCCA GCCCTCTGTG AGTAGTAATG GCACCGTCTA CCCTGCGCTA 
GTCAAAGAGA GTGGATACAC AGCTGCTCAG GGAACCTGTA ACAAGATGGC TACCTTAGAT 
GAGAACGGGA ATCAAAATGA AGCTAGCAGG CCTGGGTTTG CCTTCTGTCA GCCCCTAGAA 
CACAACTCGG TGACTCCGGT GGAGAAAAGG CCAGAACCCA CGGCCAAATA CATCTACAAA 
GTCCATTTTT CTTCAGTGCC TGAAAATGAA GACTCCTCCC TAAAGAGGCA CATCACACCT 
CCCCACGGCC ATAGTCCATA TCCCAGCGAG AGAAAGAACA TCCACGGTGG CAGCAGAGCG 
TGTTCCAATC ACCACAGTCT CTCGTCCCCC CAGGCCCAGG CCCTGCACGT GGGTGATGAC 
AGGAGGCCTT CCAGGCTCTC TCAGCCCTGG GAGGGTGACT TTCAGGAAGA TCACAATGCC 
AACCTCAGGC AGAAAGTGGA GAGGGAGGGC CAAGGCCAGG GCCTGTCGGG CAACTCTGGC 
AGGACCAGAT CGGCCTTCTC ATCTCTGCAG AATATTCCAG AGAGTCTGAG AAGACAAAGC 
AACGTGGAGC TTGGAGAAGC CCAGGAGGTT CACCCCGGGG GCAGGTCCAA GGTGGAGGAC 
CCTGGAAGGA AAGCAGGGGC TTCAGACATC AGGGGCTACC TGGACAGATC TGTTTCCTAT 
CCGAGGCCCG AGGGGAAAAT GAACGCCGTA GACTCCGTCC ACAGTGCAGA CTCCAGGTAT 
GAGGAGTCCC CGGCCCCAGC GCTGCCACAG ACATCCGGTG CGAGCCAGCG GCGGCTCAGC 
TCCAGCAGCT CCGCCGCCCC GCAGTACAGG AAGCCCCATT GCTCCGTGCT GGAGAAGGTT 
TCTAGAATAG AGGAACGAGA GCAGGGGCGC CACAGACCCC TGAGCGTGGG CAGCTCTGCA 
TACGGCCCCG GCTACAGGCC TGGGAGGACC GGCCCGACCC CCAGTACTTC CAGCAGTGAC 
CTCGACGATC CAAAAGCCGG CAGCGTCCAT TTCTCGGAGT CCACTGAACA CCTGCGCAAT 
GGGGAGCAGA ACCCCCCAAA CGGGGAGGCT AAGCAGGAAG AGGCTTCCCG GCCCCAGTGC 
AGCCATTTGA TCCGGAGGGC CCCTGCGGAC GGCCGTGGAC CTCCTGCCCG AGGCGGCGAG 
CCCTCAAGGC CGGAGGCCCG CCTGCTCCGC AGCCAGAGCA CCTTTCAGCT CTACAGTGAG 
GCGGAGAGGG AAGCCTCGTG GTCAGAGGAC AGGCCCGGTA CCCCTGAGTC GCCCCTGCTC 
GATGCGCCTT TCAGCCGCGC CTACAGAAAC AGCATCAAGG ATGCCCAGTC CCGCGTCCTG 
GGGGCCACGT CGTTTCGTCG CCGAGACCTG GAACCGGGCA CCCCCGCCAC CTCGAGGCCC 
TGGCGCCCGC GACCCGCCTC CGCCCACGTG GGGATGCGGA GCCCCGAGGC CGCGGTGCCC 
TCGTCCTCCC CGCACACCCC TCGGGAGCGA CACAGCGTGA CCCCGGCCGC GCCCCAGGCT 
GCCCGCCGAG GCCCACGCCG GCGCCTCACT GTCGAGCAGA AGAAGCGCTC CTACTCGGAG 
CCCGAGAAGA TGAACGAGGT AGGGGTCTCG GAGGAGGCCG AGCCCACGCC CTGCGGCCCG 
CCGAGGCCCG CGCAGCCGCG CTTCTCGGAG AGCACCGTGG CCGACCGGCG CCGCATCTTC 
GAGCGCGACG GCAAGGCCTG CTCCACGCTC AGCCTGTCGG GCCCCGAGCT CAAGCAGTTC 
CAGCAGAGCG CCCTGGCCGA CTACATCCAG CGTAAGACGG GCAAGCGGCC CACAGGAGCC 
GCCTGCACAC CGGAGGCCGG GCTGCGCGAG CGCGCACAGA GCGCCTACCT CCAGGCCGGC 
CCCGCGGCGC CCGATGGCCC CGGCCTGGCC TCTGCCTGTA GCCTGAGCTC CCTGCGAGAG 
CCCGAGGCGC TGCCCCGCAA GGAGCACACT CATCCGTCGG CCGCGGACGG CCCGCAGGCG 
CCCCGTGATC GCAGCAGCTC CTTCGCCAGC GGACGCCTAG TCGGGGAGCG ACGTCGCTGG 
GACCCTCAGG TCCCCAGGCA GCTGCTCAGC GGAGCGAACT GCGAACCGAG GGGCGTCCAG 
AGGATGGACG GGGCCCCGGG CGGGCCGCCT TCCTGGGGCA TGGTGGCCGG GAAGGCCGGG 
AAGTCCAAGT CAGCCGAGGA TCTGCTGGAG CGCTCCGACA CACTGGCGGT CCCTGTGCAT 
GTGAGGTCCA GGTCGTCTCC CACCTCCGAC AAGAAAGGTC AGGATGTGCT CCTGAGGGAA 
GGCAGCAACT TTGGTTTTGT AAAGGATCCA TGCTGTTTGG CTGGCCCTGG ACCTAGGTCT 
CTCAGCTGCT CAGACAAAGG CCAGAATGAG CTGGCATTGC CCCTGCACCA CCCTACGCCC 
TGCTGGAACG GCTCAGGCTG CAAAGCCACT GTGGCTTCCT CAGCTCCTCC CGAATCTTCT 
GGAGCCGCGG ATCATCTGAA GCAACGTAGA GCGCCTGGTC CTAGGCCACT CTCAGCAGGA 
ATGCACGGCC ACTTCCCAGA CGCCAGAGCT GCCTCACTCA GCTCCCCTCT GCCATCACCT 
GTCCCCTCCA GTTACCGGTC ACAGCTTGCC ATGGATCAGC AGACTGGGCA ACAGCCTCCC 
TCATCCCCTG CCTCTGCAGT GACCCAGCCT ACCAGTCCAA GAAGCCCTGA GCTCAGCAGC 
CCAGCATATG GGCTAGGGGA GGGGATGTGG AAGAGGACAT CCCTGCCTCA GCGACCCCCT 
CCCCCTTGGG TGAAATGGGC CCACGCGGTC AGAGAGGACG GCCTTGCTGA GGACACCTTG 
GCGCCTGAGT TTGCGAACCT GAAGCACTAT AGAAACCAGC CGAGTCGTCC GAGTTCCTGC 
AGCACTTCTG ACCCAGACAC TCCAGGAAGG ATCTCGCTCC GAATATCGGA GTCAGCCCTT 
CAACCTTCCC CACCGCCCCG GGGGGACTAT GATGACGAAG TGTTCATGAA GGACTTGCAC 
CCCAAGGTTA CTTCAAGCCC CACATTTGAA GCTCTTCCCC CACCCCCGCC ACCTTCGCCA 
CCGAGCGAGG AACCTCTAGT GAATGGCACA GATGACTTTC CTCCGCCTCC TCCTCCCCAA 
GCCCTGTGTG AGGTACTGCT GGATGGTGAG GCATCCACGG AAGCTGGCAG CGGCCCCTGC 
AGAATTCCCA GGGTGATGGT CACAAGGGAA GGGCACGTGC CTGGGGCAGC CCATTCCGAA 
GGTAGTCAGA TAATGACTGC CACACCACCC CAAACCTCGG CCAAAGGTTC AGAAGCCGAG 
TCCAACACGC CTTCCAGCGC GAGCGCCCAG CCTCAGCTCA ACGGTTCTCC TGGCAAGCAG 
CTCTGTCCCA GCCAGACCCG AAACCTCACT TATGAACCTG TCGAGAGAAC TCAGGATCTA 
GGAAAGAAAA CTCACGCCGA GCCTCAGAAG ACCTCAGAAG ATATCCGGAC AGAGGCTCTA 
GCCAAGGAAA TTGTCCACCA AGACAAGTCT CTGGCAGACA TTTTAGATCC TGACTCCAGG 
ATGAAGACAA CGATGGACCT GATGGAGGGT TTATTCCCTG GAGACGCCAG TGTGCTGATG 
GATAGTGGCG CAAAGAGGAA AGCCTTAGAC ATCACCGCCA GGCGTGCGGG ATGTGAAGCC 
AAGGCGAGCG ACCACAAGGA GGCAGTGAGT GTGCTGGTCA ACTGTCCTGC CTACTACAGT 
GTCTCTGCTG CCAAGGCGGA GCTGCTCAAC AAAATCAAAG ACATGCCGGA GGAGCTGCAG 
GAGGAGGAGG GGCAGGAGGA TGTCAATGAG AAAAAGGCTG AGCTTATCGG AAGCCTTACC 
CACAAGCTGG AGAGCCTCCA GGAAGCCAAG GGGAGCCTGC TCACGGACAT CAAGCTGAAC 
AATGCCCTGG GAGAGGAGGT GGAGGCTCTG ATCAGTGAGC TCTGCAAACC CAACGAGTTT 
GACAAGTACA AGATGTTCAT AGGGGACCTG GACAAGGTGG TCAACCTGCT GCTGTCCCTG 
TCTGGACGCC TGGCCCGCGT GGAGAACGTC CTCAGAGGCC TTGGTGAAGA CGCCAGCAAA 
GAGGAAAGGA GTTCTCTGAA CGAGAAAAGG AAGGTTCTGG CTGGCCAGCA TGAGGATGCA 
CGGGAGCTCA AGGAGAACCT GGACCGGAGG GAGCGCGTGG TGCTGGATAT CCTGGCCAAC 
TACCTGTCCG CCGAGCAGCT GCAGGACTAC CAGCACTTCG TGAAGATGAA GTCCACACTC 
CTCATTGAGC AGCGGAAGCT GGATGACAAG ATAAAGCTGG GCCAGGAGCA GGTCAGGTGC 
CTGCTGGAGT CGCTTCCCTC GGACTTCAGG CCCAAGGCAG GGGCCATCTC CCTCCCTCCA 
GCCCTCACCG GCCATGGGAC TCCTGGGGGG ACATCTGTAT TCGGTGGTGT TTTCCCAACG 
TTGACCTCTC CTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Shroom3 ( NM_001077596.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001071064.1 Mus musculus shroom family member 3 (Shroom3), transcript variant 3, mRNA.

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CloneID	OMu01300
Clone ID Related Accession (Same CDS sequence)	XM_006534968.2 , NM_001077595.2 , NM_001077596.2 , XM_006534968.3
Accession Version	NM_001077596.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5418bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Mus musculus(house mouse)
Product	protein Shroom3 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ045556.1, AK147493.1 and BM942679.1. On Oct 19, 2013 this sequence version replaced NM_001077596.1. Transcript Variant: This variant (3) differs in the 5' UTR and the 5' coding region and initiates translation at a downstream start codon, compared to variant 1. Variants 2 and 3 encode the same isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK147493.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGATGCAGA TATCTCAGGG CACAATGGGC CCTCCTTGGC ACCAGAGCTA CCATTCCAGC 
TCTTCGACCA GTGACCTCTC CAACTACGAC CATGCTTATC TGAGGCGGAG CCCTGACCAG 
TGCAGCTCCC AGGGGAGCAT GGAAAGCCTG GAGCCCAGTG GCGGATACCC ACCCTGCCAT 
CTCCTTTCTC CTGCCAAGTC CACGAGCAGC ATCGACCAGC TAGGCCACCT TCATAACAAA 
AGGGACTCCG CATATAGCTC CTTCTCCACC AGTTCGAGCA TCTTCGAGTA CCCACCCCCT 
GGTGGCTCTG CCCGAGAACG TTCGGGTTCC ATGGACGTGA TTTCTGCCCG GGGTGGCCTC 
CTAGAAGGGA TGAGGCAGGC AGACATCCGC TACGTCAAGA CGGTCTATGA TACCCGGAGA 
GGAGTATCTT CAGAGTATGA GGTGAACCCT TCAGCCTTGC TCCTTCAAGG TAGGGATGCC 
CATGCCTCAG CCGACAGTCA GGGCTGTGCT AAATGGCACA GCATTCCTCG GGGCAAGGGA 
ACGCCTTCCC CGTCTTGGAG CCAGCAGTGT TCTGGTTCCC TGGAGACTGC TACTGACAAC 
CTGCCCCAGA AGGCAGGCGC ACCCCTGCCC CCCACCCGCA GTGATAGCTA TGCAGCCTTC 
CGCCATCGCG AGCGTCCCAG CTCCTGGTCC AGCCTTGATC AGAAAAGGTT CTGCCGGCCT 
CAGACAAACT CTTCAGGCTC CCAGAAAACC CCATTCGCTG AGGACCAGCT GCATACAGTC 
CCAGAGAGGA GTCCAGAGAA CAGCCCCCCA GTGAAATCTA AGCATAATTA TACCCAGAAG 
GCGCAGCCTG GCCAACCTCT GCTGCCCACT GGCATCTATC CGGTACCTTC TCCGGAGCCT 
CACTTTGCCC AGGTGCCCCA GCCCTCTGTG AGTAGTAATG GCACCGTCTA CCCTGCGCTA 
GTCAAAGAGA GTGGATACAC AGCTGCTCAG GGAACCTGTA ACAAGATGGC TACCTTAGAT 
GAGAACGGGA ATCAAAATGA AGCTAGCAGG CCTGGGTTTG CCTTCTGTCA GCCCCTAGAA 
CACAACTCGG TGACTCCGGT GGAGAAAAGG CCAGAACCCA CGGCCAAATA CATCTACAAA 
GTCCATTTTT CTTCAGTGCC TGAAAATGAA GACTCCTCCC TAAAGAGGCA CATCACACCT 
CCCCACGGCC ATAGTCCATA TCCCAGCGAG AGAAAGAACA TCCACGGTGG CAGCAGAGCG 
TGTTCCAATC ACCACAGTCT CTCGTCCCCC CAGGCCCAGG CCCTGCACGT GGGTGATGAC 
AGGAGGCCTT CCAGGCTCTC TCAGCCCTGG GAGGGTGACT TTCAGGAAGA TCACAATGCC 
AACCTCAGGC AGAAAGTGGA GAGGGAGGGC CAAGGCCAGG GCCTGTCGGG CAACTCTGGC 
AGGACCAGAT CGGCCTTCTC ATCTCTGCAG AATATTCCAG AGAGTCTGAG AAGACAAAGC 
AACGTGGAGC TTGGAGAAGC CCAGGAGGTT CACCCCGGGG GCAGGTCCAA GGTGGAGGAC 
CCTGGAAGGA AAGCAGGGGC TTCAGACATC AGGGGCTACC TGGACAGATC TGTTTCCTAT 
CCGAGGCCCG AGGGGAAAAT GAACGCCGTA GACTCCGTCC ACAGTGCAGA CTCCAGGTAT 
GAGGAGTCCC CGGCCCCAGC GCTGCCACAG ACATCCGGTG CGAGCCAGCG GCGGCTCAGC 
TCCAGCAGCT CCGCCGCCCC GCAGTACAGG AAGCCCCATT GCTCCGTGCT GGAGAAGGTT 
TCTAGAATAG AGGAACGAGA GCAGGGGCGC CACAGACCCC TGAGCGTGGG CAGCTCTGCA 
TACGGCCCCG GCTACAGGCC TGGGAGGACC GGCCCGACCC CCAGTACTTC CAGCAGTGAC 
CTCGACGATC CAAAAGCCGG CAGCGTCCAT TTCTCGGAGT CCACTGAACA CCTGCGCAAT 
GGGGAGCAGA ACCCCCCAAA CGGGGAGGCT AAGCAGGAAG AGGCTTCCCG GCCCCAGTGC 
AGCCATTTGA TCCGGAGGGC CCCTGCGGAC GGCCGTGGAC CTCCTGCCCG AGGCGGCGAG 
CCCTCAAGGC CGGAGGCCCG CCTGCTCCGC AGCCAGAGCA CCTTTCAGCT CTACAGTGAG 
GCGGAGAGGG AAGCCTCGTG GTCAGAGGAC AGGCCCGGTA CCCCTGAGTC GCCCCTGCTC 
GATGCGCCTT TCAGCCGCGC CTACAGAAAC AGCATCAAGG ATGCCCAGTC CCGCGTCCTG 
GGGGCCACGT CGTTTCGTCG CCGAGACCTG GAACCGGGCA CCCCCGCCAC CTCGAGGCCC 
TGGCGCCCGC GACCCGCCTC CGCCCACGTG GGGATGCGGA GCCCCGAGGC CGCGGTGCCC 
TCGTCCTCCC CGCACACCCC TCGGGAGCGA CACAGCGTGA CCCCGGCCGC GCCCCAGGCT 
GCCCGCCGAG GCCCACGCCG GCGCCTCACT GTCGAGCAGA AGAAGCGCTC CTACTCGGAG 
CCCGAGAAGA TGAACGAGGT AGGGGTCTCG GAGGAGGCCG AGCCCACGCC CTGCGGCCCG 
CCGAGGCCCG CGCAGCCGCG CTTCTCGGAG AGCACCGTGG CCGACCGGCG CCGCATCTTC 
GAGCGCGACG GCAAGGCCTG CTCCACGCTC AGCCTGTCGG GCCCCGAGCT CAAGCAGTTC 
CAGCAGAGCG CCCTGGCCGA CTACATCCAG CGTAAGACGG GCAAGCGGCC CACAGGAGCC 
GCCTGCACAC CGGAGGCCGG GCTGCGCGAG CGCGCACAGA GCGCCTACCT CCAGGCCGGC 
CCCGCGGCGC CCGATGGCCC CGGCCTGGCC TCTGCCTGTA GCCTGAGCTC CCTGCGAGAG 
CCCGAGGCGC TGCCCCGCAA GGAGCACACT CATCCGTCGG CCGCGGACGG CCCGCAGGCG 
CCCCGTGATC GCAGCAGCTC CTTCGCCAGC GGACGCCTAG TCGGGGAGCG ACGTCGCTGG 
GACCCTCAGG TCCCCAGGCA GCTGCTCAGC GGAGCGAACT GCGAACCGAG GGGCGTCCAG 
AGGATGGACG GGGCCCCGGG CGGGCCGCCT TCCTGGGGCA TGGTGGCCGG GAAGGCCGGG 
AAGTCCAAGT CAGCCGAGGA TCTGCTGGAG CGCTCCGACA CACTGGCGGT CCCTGTGCAT 
GTGAGGTCCA GGTCGTCTCC CACCTCCGAC AAGAAAGGTC AGGATGTGCT CCTGAGGGAA 
GGCAGCAACT TTGGTTTTGT AAAGGATCCA TGCTGTTTGG CTGGCCCTGG ACCTAGGTCT 
CTCAGCTGCT CAGACAAAGG CCAGAATGAG CTGGCATTGC CCCTGCACCA CCCTACGCCC 
TGCTGGAACG GCTCAGGCTG CAAAGCCACT GTGGCTTCCT CAGCTCCTCC CGAATCTTCT 
GGAGCCGCGG ATCATCTGAA GCAACGTAGA GCGCCTGGTC CTAGGCCACT CTCAGCAGGA 
ATGCACGGCC ACTTCCCAGA CGCCAGAGCT GCCTCACTCA GCTCCCCTCT GCCATCACCT 
GTCCCCTCCA GTTACCGGTC ACAGCTTGCC ATGGATCAGC AGACTGGGCA ACAGCCTCCC 
TCATCCCCTG CCTCTGCAGT GACCCAGCCT ACCAGTCCAA GAAGCCCTGA GCTCAGCAGC 
CCAGCATATG GGCTAGGGGA GGGGATGTGG AAGAGGACAT CCCTGCCTCA GCGACCCCCT 
CCCCCTTGGG TGAAATGGGC CCACGCGGTC AGAGAGGACG GCCTTGCTGA GGACACCTTG 
GCGCCTGAGT TTGCGAACCT GAAGCACTAT AGAAACCAGC CGAGTCGTCC GAGTTCCTGC 
AGCACTTCTG ACCCAGACAC TCCAGGAAGG ATCTCGCTCC GAATATCGGA GTCAGCCCTT 
CAACCTTCCC CACCGCCCCG GGGGGACTAT GATGACGAAG TGTTCATGAA GGACTTGCAC 
CCCAAGGTTA CTTCAAGCCC CACATTTGAA GCTCTTCCCC CACCCCCGCC ACCTTCGCCA 
CCGAGCGAGG AACCTCTAGT GAATGGCACA GATGACTTTC CTCCGCCTCC TCCTCCCCAA 
GCCCTGTGTG AGGTACTGCT GGATGGTGAG GCATCCACGG AAGCTGGCAG CGGCCCCTGC 
AGAATTCCCA GGGTGATGGT CACAAGGGAA GGGCACGTGC CTGGGGCAGC CCATTCCGAA 
GGTAGTCAGA TAATGACTGC CACACCACCC CAAACCTCGG CCAAAGGTTC AGAAGCCGAG 
TCCAACACGC CTTCCAGCGC GAGCGCCCAG CCTCAGCTCA ACGGTTCTCC TGGCAAGCAG 
CTCTGTCCCA GCCAGACCCG AAACCTCACT TATGAACCTG TCGAGAGAAC TCAGGATCTA 
GGAAAGAAAA CTCACGCCGA GCCTCAGAAG ACCTCAGAAG ATATCCGGAC AGAGGCTCTA 
GCCAAGGAAA TTGTCCACCA AGACAAGTCT CTGGCAGACA TTTTAGATCC TGACTCCAGG 
ATGAAGACAA CGATGGACCT GATGGAGGGT TTATTCCCTG GAGACGCCAG TGTGCTGATG 
GATAGTGGCG CAAAGAGGAA AGCCTTAGAC ATCACCGCCA GGCGTGCGGG ATGTGAAGCC 
AAGGCGAGCG ACCACAAGGA GGCAGTGAGT GTGCTGGTCA ACTGTCCTGC CTACTACAGT 
GTCTCTGCTG CCAAGGCGGA GCTGCTCAAC AAAATCAAAG ACATGCCGGA GGAGCTGCAG 
GAGGAGGAGG GGCAGGAGGA TGTCAATGAG AAAAAGGCTG AGCTTATCGG AAGCCTTACC 
CACAAGCTGG AGAGCCTCCA GGAAGCCAAG GGGAGCCTGC TCACGGACAT CAAGCTGAAC 
AATGCCCTGG GAGAGGAGGT GGAGGCTCTG ATCAGTGAGC TCTGCAAACC CAACGAGTTT 
GACAAGTACA AGATGTTCAT AGGGGACCTG GACAAGGTGG TCAACCTGCT GCTGTCCCTG 
TCTGGACGCC TGGCCCGCGT GGAGAACGTC CTCAGAGGCC TTGGTGAAGA CGCCAGCAAA 
GAGGAAAGGA GTTCTCTGAA CGAGAAAAGG AAGGTTCTGG CTGGCCAGCA TGAGGATGCA 
CGGGAGCTCA AGGAGAACCT GGACCGGAGG GAGCGCGTGG TGCTGGATAT CCTGGCCAAC 
TACCTGTCCG CCGAGCAGCT GCAGGACTAC CAGCACTTCG TGAAGATGAA GTCCACACTC 
CTCATTGAGC AGCGGAAGCT GGATGACAAG ATAAAGCTGG GCCAGGAGCA GGTCAGGTGC 
CTGCTGGAGT CGCTTCCCTC GGACTTCAGG CCCAAGGCAG GGGCCATCTC CCTCCCTCCA 
GCCCTCACCG GCCATGGGAC TCCTGGGGGG ACATCTGTAT TCGGTGGTGT TTTCCCAACG 
TTGACCTCTC CTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001071064.1
CDS	434..5851
Translation	MMQISQGTMGPPWHQSYHSSSSTSDLSNYDHAYLRRSPDQCSSQGSMESLEPSGGYPPCHLLSPAKSTSSIDQLGHLHNKRDSAYSSFSTSSSIFEYPPPGGSARERSGSMDVISARGGLLEGMRQADIRYVKTVYDTRRGVSSEYEVNPSALLLQGRDAHASADSQGCAKWHSIPRGKGTPSPSWSQQCSGSLETATDNLPQKAGAPLPPTRSDSYAAFRHRERPSSWSSLDQKRFCRPQTNSSGSQKTPFAEDQLHTVPERSPENSPPVKSKHNYTQKAQPGQPLLPTGIYPVPSPEPHFAQVPQPSVSSNGTVYPALVKESGYTAAQGTCNKMATLDENGNQNEASRPGFAFCQPLEHNSVTPVEKRPEPTAKYIYKVHFSSVPENEDSSLKRHITPPHGHSPYPSERKNIHGGSRACSNHHSLSSPQAQALHVGDDRRPSRLSQPWEGDFQEDHNANLRQKVEREGQGQGLSGNSGRTRSAFSSLQNIPESLRRQSNVELGEAQEVHPGGRSKVEDPGRKAGASDIRGYLDRSVSYPRPEGKMNAVDSVHSADSRYEESPAPALPQTSGASQRRLSSSSSAAPQYRKPHCSVLEKVSRIEEREQGRHRPLSVGSSAYGPGYRPGRTGPTPSTSSSDLDDPKAGSVHFSESTEHLRNGEQNPPNGEAKQEEASRPQCSHLIRRAPADGRGPPARGGEPSRPEARLLRSQSTFQLYSEAEREASWSEDRPGTPESPLLDAPFSRAYRNSIKDAQSRVLGATSFRRRDLEPGTPATSRPWRPRPASAHVGMRSPEAAVPSSSPHTPRERHSVTPAAPQAARRGPRRRLTVEQKKRSYSEPEKMNEVGVSEEAEPTPCGPPRPAQPRFSESTVADRRRIFERDGKACSTLSLSGPELKQFQQSALADYIQRKTGKRPTGAACTPEAGLRERAQSAYLQAGPAAPDGPGLASACSLSSLREPEALPRKEHTHPSAADGPQAPRDRSSSFASGRLVGERRRWDPQVPRQLLSGANCEPRGVQRMDGAPGGPPSWGMVAGKAGKSKSAEDLLERSDTLAVPVHVRSRSSPTSDKKGQDVLLREGSNFGFVKDPCCLAGPGPRSLSCSDKGQNELALPLHHPTPCWNGSGCKATVASSAPPESSGAADHLKQRRAPGPRPLSAGMHGHFPDARAASLSSPLPSPVPSSYRSQLAMDQQTGQQPPSSPASAVTQPTSPRSPELSSPAYGLGEGMWKRTSLPQRPPPPWVKWAHAVREDGLAEDTLAPEFANLKHYRNQPSRPSSCSTSDPDTPGRISLRISESALQPSPPPRGDYDDEVFMKDLHPKVTSSPTFEALPPPPPPSPPSEEPLVNGTDDFPPPPPPQALCEVLLDGEASTEAGSGPCRIPRVMVTREGHVPGAAHSEGSQIMTATPPQTSAKGSEAESNTPSSASAQPQLNGSPGKQLCPSQTRNLTYEPVERTQDLGKKTHAEPQKTSEDIRTEALAKEIVHQDKSLADILDPDSRMKTTMDLMEGLFPGDASVLMDSGAKRKALDITARRAGCEAKASDHKEAVSVLVNCPAYYSVSAAKAELLNKIKDMPEELQEEEGQEDVNEKKAELIGSLTHKLESLQEAKGSLLTDIKLNNALGEEVEALISELCKPNEFDKYKMFIGDLDKVVNLLLSLSGRLARVENVLRGLGEDASKEERSSLNEKRKVLAGQHEDARELKENLDRRERVVLDILANYLSAEQLQDYQHFVKMKSTLLIEQRKLDDKIKLGQEQVRCLLESLPSDFRPKAGAISLPPALTGHGTPGGTSVFGGVFPTLTSPL

Target ORF information:

RefSeq Version	NM_001077596.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus shroom family member 3 (Shroom3), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001077596.2

ORF Insert Sequence:

ATGATGCAGA TATCTCAGGG CACAATGGGC CCTCCTTGGC ACCAGAGCTA CCATTCCAGC 
TCTTCGACCA GTGACCTCTC CAACTACGAC CATGCTTATC TGAGGCGGAG CCCTGACCAG 
TGCAGCTCCC AGGGGAGCAT GGAAAGCCTG GAGCCCAGTG GCGGATACCC ACCCTGCCAT 
CTCCTTTCTC CTGCCAAGTC CACGAGCAGC ATCGACCAGC TAGGCCACCT TCATAACAAA 
AGGGACTCCG CATATAGCTC CTTCTCCACC AGTTCGAGCA TCTTCGAGTA CCCACCCCCT 
GGTGGCTCTG CCCGAGAACG TTCGGGTTCC ATGGACGTGA TTTCTGCCCG GGGTGGCCTC 
CTAGAAGGGA TGAGGCAGGC AGACATCCGC TACGTCAAGA CGGTCTATGA TACCCGGAGA 
GGAGTATCTT CAGAGTATGA GGTGAACCCT TCAGCCTTGC TCCTTCAAGG TAGGGATGCC 
CATGCCTCAG CCGACAGTCA GGGCTGTGCT AAATGGCACA GCATTCCTCG GGGCAAGGGA 
ACGCCTTCCC CGTCTTGGAG CCAGCAGTGT TCTGGTTCCC TGGAGACTGC TACTGACAAC 
CTGCCCCAGA AGGCAGGCGC ACCCCTGCCC CCCACCCGCA GTGATAGCTA TGCAGCCTTC 
CGCCATCGCG AGCGTCCCAG CTCCTGGTCC AGCCTTGATC AGAAAAGGTT CTGCCGGCCT 
CAGACAAACT CTTCAGGCTC CCAGAAAACC CCATTCGCTG AGGACCAGCT GCATACAGTC 
CCAGAGAGGA GTCCAGAGAA CAGCCCCCCA GTGAAATCTA AGCATAATTA TACCCAGAAG 
GCGCAGCCTG GCCAACCTCT GCTGCCCACT GGCATCTATC CGGTACCTTC TCCGGAGCCT 
CACTTTGCCC AGGTGCCCCA GCCCTCTGTG AGTAGTAATG GCACCGTCTA CCCTGCGCTA 
GTCAAAGAGA GTGGATACAC AGCTGCTCAG GGAACCTGTA ACAAGATGGC TACCTTAGAT 
GAGAACGGGA ATCAAAATGA AGCTAGCAGG CCTGGGTTTG CCTTCTGTCA GCCCCTAGAA 
CACAACTCGG TGACTCCGGT GGAGAAAAGG CCAGAACCCA CGGCCAAATA CATCTACAAA 
GTCCATTTTT CTTCAGTGCC TGAAAATGAA GACTCCTCCC TAAAGAGGCA CATCACACCT 
CCCCACGGCC ATAGTCCATA TCCCAGCGAG AGAAAGAACA TCCACGGTGG CAGCAGAGCG 
TGTTCCAATC ACCACAGTCT CTCGTCCCCC CAGGCCCAGG CCCTGCACGT GGGTGATGAC 
AGGAGGCCTT CCAGGCTCTC TCAGCCCTGG GAGGGTGACT TTCAGGAAGA TCACAATGCC 
AACCTCAGGC AGAAAGTGGA GAGGGAGGGC CAAGGCCAGG GCCTGTCGGG CAACTCTGGC 
AGGACCAGAT CGGCCTTCTC ATCTCTGCAG AATATTCCAG AGAGTCTGAG AAGACAAAGC 
AACGTGGAGC TTGGAGAAGC CCAGGAGGTT CACCCCGGGG GCAGGTCCAA GGTGGAGGAC 
CCTGGAAGGA AAGCAGGGGC TTCAGACATC AGGGGCTACC TGGACAGATC TGTTTCCTAT 
CCGAGGCCCG AGGGGAAAAT GAACGCCGTA GACTCCGTCC ACAGTGCAGA CTCCAGGTAT 
GAGGAGTCCC CGGCCCCAGC GCTGCCACAG ACATCCGGTG CGAGCCAGCG GCGGCTCAGC 
TCCAGCAGCT CCGCCGCCCC GCAGTACAGG AAGCCCCATT GCTCCGTGCT GGAGAAGGTT 
TCTAGAATAG AGGAACGAGA GCAGGGGCGC CACAGACCCC TGAGCGTGGG CAGCTCTGCA 
TACGGCCCCG GCTACAGGCC TGGGAGGACC GGCCCGACCC CCAGTACTTC CAGCAGTGAC 
CTCGACGATC CAAAAGCCGG CAGCGTCCAT TTCTCGGAGT CCACTGAACA CCTGCGCAAT 
GGGGAGCAGA ACCCCCCAAA CGGGGAGGCT AAGCAGGAAG AGGCTTCCCG GCCCCAGTGC 
AGCCATTTGA TCCGGAGGGC CCCTGCGGAC GGCCGTGGAC CTCCTGCCCG AGGCGGCGAG 
CCCTCAAGGC CGGAGGCCCG CCTGCTCCGC AGCCAGAGCA CCTTTCAGCT CTACAGTGAG 
GCGGAGAGGG AAGCCTCGTG GTCAGAGGAC AGGCCCGGTA CCCCTGAGTC GCCCCTGCTC 
GATGCGCCTT TCAGCCGCGC CTACAGAAAC AGCATCAAGG ATGCCCAGTC CCGCGTCCTG 
GGGGCCACGT CGTTTCGTCG CCGAGACCTG GAACCGGGCA CCCCCGCCAC CTCGAGGCCC 
TGGCGCCCGC GACCCGCCTC CGCCCACGTG GGGATGCGGA GCCCCGAGGC CGCGGTGCCC 
TCGTCCTCCC CGCACACCCC TCGGGAGCGA CACAGCGTGA CCCCGGCCGC GCCCCAGGCT 
GCCCGCCGAG GCCCACGCCG GCGCCTCACT GTCGAGCAGA AGAAGCGCTC CTACTCGGAG 
CCCGAGAAGA TGAACGAGGT AGGGGTCTCG GAGGAGGCCG AGCCCACGCC CTGCGGCCCG 
CCGAGGCCCG CGCAGCCGCG CTTCTCGGAG AGCACCGTGG CCGACCGGCG CCGCATCTTC 
GAGCGCGACG GCAAGGCCTG CTCCACGCTC AGCCTGTCGG GCCCCGAGCT CAAGCAGTTC 
CAGCAGAGCG CCCTGGCCGA CTACATCCAG CGTAAGACGG GCAAGCGGCC CACAGGAGCC 
GCCTGCACAC CGGAGGCCGG GCTGCGCGAG CGCGCACAGA GCGCCTACCT CCAGGCCGGC 
CCCGCGGCGC CCGATGGCCC CGGCCTGGCC TCTGCCTGTA GCCTGAGCTC CCTGCGAGAG 
CCCGAGGCGC TGCCCCGCAA GGAGCACACT CATCCGTCGG CCGCGGACGG CCCGCAGGCG 
CCCCGTGATC GCAGCAGCTC CTTCGCCAGC GGACGCCTAG TCGGGGAGCG ACGTCGCTGG 
GACCCTCAGG TCCCCAGGCA GCTGCTCAGC GGAGCGAACT GCGAACCGAG GGGCGTCCAG 
AGGATGGACG GGGCCCCGGG CGGGCCGCCT TCCTGGGGCA TGGTGGCCGG GAAGGCCGGG 
AAGTCCAAGT CAGCCGAGGA TCTGCTGGAG CGCTCCGACA CACTGGCGGT CCCTGTGCAT 
GTGAGGTCCA GGTCGTCTCC CACCTCCGAC AAGAAAGGTC AGGATGTGCT CCTGAGGGAA 
GGCAGCAACT TTGGTTTTGT AAAGGATCCA TGCTGTTTGG CTGGCCCTGG ACCTAGGTCT 
CTCAGCTGCT CAGACAAAGG CCAGAATGAG CTGGCATTGC CCCTGCACCA CCCTACGCCC 
TGCTGGAACG GCTCAGGCTG CAAAGCCACT GTGGCTTCCT CAGCTCCTCC CGAATCTTCT 
GGAGCCGCGG ATCATCTGAA GCAACGTAGA GCGCCTGGTC CTAGGCCACT CTCAGCAGGA 
ATGCACGGCC ACTTCCCAGA CGCCAGAGCT GCCTCACTCA GCTCCCCTCT GCCATCACCT 
GTCCCCTCCA GTTACCGGTC ACAGCTTGCC ATGGATCAGC AGACTGGGCA ACAGCCTCCC 
TCATCCCCTG CCTCTGCAGT GACCCAGCCT ACCAGTCCAA GAAGCCCTGA GCTCAGCAGC 
CCAGCATATG GGCTAGGGGA GGGGATGTGG AAGAGGACAT CCCTGCCTCA GCGACCCCCT 
CCCCCTTGGG TGAAATGGGC CCACGCGGTC AGAGAGGACG GCCTTGCTGA GGACACCTTG 
GCGCCTGAGT TTGCGAACCT GAAGCACTAT AGAAACCAGC CGAGTCGTCC GAGTTCCTGC 
AGCACTTCTG ACCCAGACAC TCCAGGAAGG ATCTCGCTCC GAATATCGGA GTCAGCCCTT 
CAACCTTCCC CACCGCCCCG GGGGGACTAT GATGACGAAG TGTTCATGAA GGACTTGCAC 
CCCAAGGTTA CTTCAAGCCC CACATTTGAA GCTCTTCCCC CACCCCCGCC ACCTTCGCCA 
CCGAGCGAGG AACCTCTAGT GAATGGCACA GATGACTTTC CTCCGCCTCC TCCTCCCCAA 
GCCCTGTGTG AGGTACTGCT GGATGGTGAG GCATCCACGG AAGCTGGCAG CGGCCCCTGC 
AGAATTCCCA GGGTGATGGT CACAAGGGAA GGGCACGTGC CTGGGGCAGC CCATTCCGAA 
GGTAGTCAGA TAATGACTGC CACACCACCC CAAACCTCGG CCAAAGGTTC AGAAGCCGAG 
TCCAACACGC CTTCCAGCGC GAGCGCCCAG CCTCAGCTCA ACGGTTCTCC TGGCAAGCAG 
CTCTGTCCCA GCCAGACCCG AAACCTCACT TATGAACCTG TCGAGAGAAC TCAGGATCTA 
GGAAAGAAAA CTCACGCCGA GCCTCAGAAG ACCTCAGAAG ATATCCGGAC AGAGGCTCTA 
GCCAAGGAAA TTGTCCACCA AGACAAGTCT CTGGCAGACA TTTTAGATCC TGACTCCAGG 
ATGAAGACAA CGATGGACCT GATGGAGGGT TTATTCCCTG GAGACGCCAG TGTGCTGATG 
GATAGTGGCG CAAAGAGGAA AGCCTTAGAC ATCACCGCCA GGCGTGCGGG ATGTGAAGCC 
AAGGCGAGCG ACCACAAGGA GGCAGTGAGT GTGCTGGTCA ACTGTCCTGC CTACTACAGT 
GTCTCTGCTG CCAAGGCGGA GCTGCTCAAC AAAATCAAAG ACATGCCGGA GGAGCTGCAG 
GAGGAGGAGG GGCAGGAGGA TGTCAATGAG AAAAAGGCTG AGCTTATCGG AAGCCTTACC 
CACAAGCTGG AGAGCCTCCA GGAAGCCAAG GGGAGCCTGC TCACGGACAT CAAGCTGAAC 
AATGCCCTGG GAGAGGAGGT GGAGGCTCTG ATCAGTGAGC TCTGCAAACC CAACGAGTTT 
GACAAGTACA AGATGTTCAT AGGGGACCTG GACAAGGTGG TCAACCTGCT GCTGTCCCTG 
TCTGGACGCC TGGCCCGCGT GGAGAACGTC CTCAGAGGCC TTGGTGAAGA CGCCAGCAAA 
GAGGAAAGGA GTTCTCTGAA CGAGAAAAGG AAGGTTCTGG CTGGCCAGCA TGAGGATGCA 
CGGGAGCTCA AGGAGAACCT GGACCGGAGG GAGCGCGTGG TGCTGGATAT CCTGGCCAAC 
TACCTGTCCG CCGAGCAGCT GCAGGACTAC CAGCACTTCG TGAAGATGAA GTCCACACTC 
CTCATTGAGC AGCGGAAGCT GGATGACAAG ATAAAGCTGG GCCAGGAGCA GGTCAGGTGC 
CTGCTGGAGT CGCTTCCCTC GGACTTCAGG CCCAAGGCAG GGGCCATCTC CCTCCCTCCA 
GCCCTCACCG GCCATGGGAC TCCTGGGGGG ACATCTGTAT TCGGTGGTGT TTTCCCAACG 
TTGACCTCTC CTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Shroom3 ( NM_015756.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_056571.2 Mus musculus shroom family member 3 (Shroom3), transcript variant 1, mRNA.

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CloneID	OMu10221
Clone ID Related Accession (Same CDS sequence)	NM_015756.2
Accession Version	NM_015756.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5943bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	protein Shroom3 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC124560.4, AC125233.4, AK147493.1 and BM942679.1. On Nov 8, 2006 this sequence version replaced NM_015756.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF199421.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384, SAMN00849385 [ECO:0000348] ##Evidence-Data-END##

ATGAAGACTC CGGAGAACTT GGAGGAGCCT AGCGCCACGC CAAACCCCAG TAGGACCCCC 
ACGGAGAGAT TCGTTTATCT GGAAGCGCTC CTAGAGGGAG GGGCTCCCTG GGGCTTCACC 
CTGAAAGGTG GCCTGGAGCG TGGAGAACCG TTAATCATTT CCAAGATTGA AGAAGGGGGC 
AAAGCCGACT CAGTGAGCTC CGGACTGCAA GCTGGGGACG AAGTCATACA CATCAATGAG 
GTGGCGCTGA GCAGTTCCAG GAGAGAGGCT GTCTCCCTAG TGAAAGGGTC CTACAAGACC 
CTGCGGCTGG TGGTGCGCAG AGACGTGTGT GCGGCCCCGG GCCATGCTGA TCCTGGTACC 
TCTAAGTCCC TCAGCTCAGA GCTGCTCACC TGCAGTCCCC AGCACCGGAA AGCAACATGG 
TCAGGAGGGG TGAAGCTTCG GCTAAAGCAG AGGTGCAGTG AGCCTGCCAC TCGCCCTCAC 
TCGTGGCATA CCACCAAATT TGGGGAGACA CAGCCTGATG TCAGCATGAT GCAGATATCT 
CAGGGCACAA TGGGCCCTCC TTGGCACCAG AGCTACCATT CCAGCTCTTC GACCAGTGAC 
CTCTCCAACT ACGACCATGC TTATCTGAGG CGGAGCCCTG ACCAGTGCAG CTCCCAGGGG 
AGCATGGAAA GCCTGGAGCC CAGTGGCGGA TACCCACCCT GCCATCTCCT TTCTCCTGCC 
AAGTCCACGA GCAGCATCGA CCAGCTAGGC CACCTTCATA ACAAAAGGGA CTCCGCATAT 
AGCTCCTTCT CCACCAGTTC GAGCATCTTC GAGTACCCAC CCCCTGGTGG CTCTGCCCGA 
GAACGTTCGG GTTCCATGGA CGTGATTTCT GCCCGGGGTG GCCTCCTAGA AGGGATGAGG 
CAGGCAGACA TCCGCTACGT CAAGACGGTC TATGATACCC GGAGAGGAGT ATCTTCAGAG 
TATGAGGTGA ACCCTTCAGC CTTGCTCCTT CAAGGTAGGG ATGCCCATGC CTCAGCCGAC 
AGTCAGGGCT GTGCTAAATG GCACAGCATT CCTCGGGGCA AGGGAACGCC TTCCCCGTCT 
TGGAGCCAGC AGTGTTCTGG TTCCCTGGAG ACTGCTACTG ACAACCTGCC CCAGAAGGCA 
GGCGCACCCC TGCCCCCCAC CCGCAGTGAT AGCTATGCAG CCTTCCGCCA TCGCGAGCGT 
CCCAGCTCCT GGTCCAGCCT TGATCAGAAA AGGTTCTGCC GGCCTCAGAC AAACTCTTCA 
GGCTCCCAGA AAACCCCATT CGCTGAGGAC CAGCTGCATA CAGTCCCAGA GAGGAGTCCA 
GAGAACAGCC CCCCAGTGAA ATCTAAGCAT AATTATACCC AGAAGGCGCA GCCTGGCCAA 
CCTCTGCTGC CCACTGGCAT CTATCCGGTA CCTTCTCCGG AGCCTCACTT TGCCCAGGTG 
CCCCAGCCCT CTGTGAGTAG TAATGGCACC GTCTACCCTG CGCTAGTCAA AGAGAGTGGA 
TACACAGCTG CTCAGGGAAC CTGTAACAAG ATGGCTACCT TAGATGAGAA CGGGAATCAA 
AATGAAGCTA GCAGGCCTGG GTTTGCCTTC TGTCAGCCCC TAGAACACAA CTCGGTGACT 
CCGGTGGAGA AAAGGCCAGA ACCCACGGCC AAATACATCT ACAAAGTCCA TTTTTCTTCA 
GTGCCTGAAA ATGAAGACTC CTCCCTAAAG AGGCACATCA CACCTCCCCA CGGCCATAGT 
CCATATCCCA GCGAGAGAAA GAACATCCAC GGTGGCAGCA GAGCGTGTTC CAATCACCAC 
AGTCTCTCGT CCCCCCAGGC CCAGGCCCTG CACGTGGGTG ATGACAGGAG GCCTTCCAGG 
CTCTCTCAGC CCTGGGAGGG TGACTTTCAG GAAGATCACA ATGCCAACCT CAGGCAGAAA 
GTGGAGAGGG AGGGCCAAGG CCAGGGCCTG TCGGGCAACT CTGGCAGGAC CAGATCGGCC 
TTCTCATCTC TGCAGAATAT TCCAGAGAGT CTGAGAAGAC AAAGCAACGT GGAGCTTGGA 
GAAGCCCAGG AGGTTCACCC CGGGGGCAGG TCCAAGGTGG AGGACCCTGG AAGGAAAGCA 
GGGGCTTCAG ACATCAGGGG CTACCTGGAC AGATCTGTTT CCTATCCGAG GCCCGAGGGG 
AAAATGAACG CCGTAGACTC CGTCCACAGT GCAGACTCCA GGTATGAGGA GTCCCCGGCC 
CCAGCGCTGC CACAGACATC CGGTGCGAGC CAGCGGCGGC TCAGCTCCAG CAGCTCCGCC 
GCCCCGCAGT ACAGGAAGCC CCATTGCTCC GTGCTGGAGA AGGTTTCTAG AATAGAGGAA 
CGAGAGCAGG GGCGCCACAG ACCCCTGAGC GTGGGCAGCT CTGCATACGG CCCCGGCTAC 
AGGCCTGGGA GGACCGGCCC GACCCCCAGT ACTTCCAGCA GTGACCTCGA CGATCCAAAA 
GCCGGCAGCG TCCATTTCTC GGAGTCCACT GAACACCTGC GCAATGGGGA GCAGAACCCC 
CCAAACGGGG AGGCTAAGCA GGAAGAGGCT TCCCGGCCCC AGTGCAGCCA TTTGATCCGG 
AGGGCCCCTG CGGACGGCCG TGGACCTCCT GCCCGAGGCG GCGAGCCCTC AAGGCCGGAG 
GCCCGCCTGC TCCGCAGCCA GAGCACCTTT CAGCTCTACA GTGAGGCGGA GAGGGAAGCC 
TCGTGGTCAG AGGACAGGCC CGGTACCCCT GAGTCGCCCC TGCTCGATGC GCCTTTCAGC 
CGCGCCTACA GAAACAGCAT CAAGGATGCC CAGTCCCGCG TCCTGGGGGC CACGTCGTTT 
CGTCGCCGAG ACCTGGAACC GGGCACCCCC GCCACCTCGA GGCCCTGGCG CCCGCGACCC 
GCCTCCGCCC ACGTGGGGAT GCGGAGCCCC GAGGCCGCGG TGCCCTCGTC CTCCCCGCAC 
ACCCCTCGGG AGCGACACAG CGTGACCCCG GCCGCGCCCC AGGCTGCCCG CCGAGGCCCA 
CGCCGGCGCC TCACTGTCGA GCAGAAGAAG CGCTCCTACT CGGAGCCCGA GAAGATGAAC 
GAGGTAGGGG TCTCGGAGGA GGCCGAGCCC ACGCCCTGCG GCCCGCCGAG GCCCGCGCAG 
CCGCGCTTCT CGGAGAGCAC CGTGGCCGAC CGGCGCCGCA TCTTCGAGCG CGACGGCAAG 
GCCTGCTCCA CGCTCAGCCT GTCGGGCCCC GAGCTCAAGC AGTTCCAGCA GAGCGCCCTG 
GCCGACTACA TCCAGCGTAA GACGGGCAAG CGGCCCACAG GAGCCGCCTG CACACCGGAG 
GCCGGGCTGC GCGAGCGCGC ACAGAGCGCC TACCTCCAGG CCGGCCCCGC GGCGCCCGAT 
GGCCCCGGCC TGGCCTCTGC CTGTAGCCTG AGCTCCCTGC GAGAGCCCGA GGCGCTGCCC 
CGCAAGGAGC ACACTCATCC GTCGGCCGCG GACGGCCCGC AGGCGCCCCG TGATCGCAGC 
AGCTCCTTCG CCAGCGGACG CCTAGTCGGG GAGCGACGTC GCTGGGACCC TCAGGTCCCC 
AGGCAGCTGC TCAGCGGAGC GAACTGCGAA CCGAGGGGCG TCCAGAGGAT GGACGGGGCC 
CCGGGCGGGC CGCCTTCCTG GGGCATGGTG GCCGGGAAGG CCGGGAAGTC CAAGTCAGCC 
GAGGATCTGC TGGAGCGCTC CGACACACTG GCGGTCCCTG TGCATGTGAG GTCCAGGTCG 
TCTCCCACCT CCGACAAGAA AGGTCAGGAT GTGCTCCTGA GGGAAGGCAG CAACTTTGGT 
TTTGTAAAGG ATCCATGCTG TTTGGCTGGC CCTGGACCTA GGTCTCTCAG CTGCTCAGAC 
AAAGGCCAGA ATGAGCTGGC ATTGCCCCTG CACCACCCTA CGCCCTGCTG GAACGGCTCA 
GGCTGCAAAG CCACTGTGGC TTCCTCAGCT CCTCCCGAAT CTTCTGGAGC CGCGGATCAT 
CTGAAGCAAC GTAGAGCGCC TGGTCCTAGG CCACTCTCAG CAGGAATGCA CGGCCACTTC 
CCAGACGCCA GAGCTGCCTC ACTCAGCTCC CCTCTGCCAT CACCTGTCCC CTCCAGTTAC 
CGGTCACAGC TTGCCATGGA TCAGCAGACT GGGCAACAGC CTCCCTCATC CCCTGCCTCT 
GCAGTGACCC AGCCTACCAG TCCAAGAAGC CCTGAGCTCA GCAGCCCAGC ATATGGGCTA 
GGGGAGGGGA TGTGGAAGAG GACATCCCTG CCTCAGCGAC CCCCTCCCCC TTGGGTGAAA 
TGGGCCCACG CGGTCAGAGA GGACGGCCTT GCTGAGGACA CCTTGGCGCC TGAGTTTGCG 
AACCTGAAGC ACTATAGAAA CCAGCCGAGT CGTCCGAGTT CCTGCAGCAC TTCTGACCCA 
GACACTCCAG GAAGGATCTC GCTCCGAATA TCGGAGTCAG CCCTTCAACC TTCCCCACCG 
CCCCGGGGGG ACTATGATGA CGAAGTGTTC ATGAAGGACT TGCACCCCAA GGTTACTTCA 
AGCCCCACAT TTGAAGCTCT TCCCCCACCC CCGCCACCTT CGCCACCGAG CGAGGAACCT 
CTAGTGAATG GCACAGATGA CTTTCCTCCG CCTCCTCCTC CCCAAGCCCT GTGTGAGGTA 
CTGCTGGATG GTGAGGCATC CACGGAAGCT GGCAGCGGCC CCTGCAGAAT TCCCAGGGTG 
ATGGTCACAA GGGAAGGGCA CGTGCCTGGG GCAGCCCATT CCGAAGGTAG TCAGATAATG 
ACTGCCACAC CACCCCAAAC CTCGGCCAAA GGTTCAGAAG CCGAGTCCAA CACGCCTTCC 
AGCGCGAGCG CCCAGCCTCA GCTCAACGGT TCTCCTGGCA AGCAGCTCTG TCCCAGCCAG 
ACCCGAAACC TCACTTATGA ACCTGTCGAG AGAACTCAGG ATCTAGGAAA GAAAACTCAC 
GCCGAGCCTC AGAAGACCTC AGAAGATATC CGGACAGAGG CTCTAGCCAA GGAAATTGTC 
CACCAAGACA AGTCTCTGGC AGACATTTTA GATCCTGACT CCAGGATGAA GACAACGATG 
GACCTGATGG AGGGTTTATT CCCTGGAGAC GCCAGTGTGC TGATGGATAG TGGCGCAAAG 
AGGAAAGCCT TAGACATCAC CGCCAGGCGT GCGGGATGTG AAGCCAAGGC GAGCGACCAC 
AAGGAGGCAG TGAGTGTGCT GGTCAACTGT CCTGCCTACT ACAGTGTCTC TGCTGCCAAG 
GCGGAGCTGC TCAACAAAAT CAAAGACATG CCGGAGGAGC TGCAGGAGGA GGAGGGGCAG 
GAGGATGTCA ATGAGAAAAA GGCTGAGCTT ATCGGAAGCC TTACCCACAA GCTGGAGAGC 
CTCCAGGAAG CCAAGGGGAG CCTGCTCACG GACATCAAGC TGAACAATGC CCTGGGAGAG 
GAGGTGGAGG CTCTGATCAG TGAGCTCTGC AAACCCAACG AGTTTGACAA GTACAAGATG 
TTCATAGGGG ACCTGGACAA GGTGGTCAAC CTGCTGCTGT CCCTGTCTGG ACGCCTGGCC 
CGCGTGGAGA ACGTCCTCAG AGGCCTTGGT GAAGACGCCA GCAAAGAGGA AAGGAGTTCT 
CTGAACGAGA AAAGGAAGGT TCTGGCTGGC CAGCATGAGG ATGCACGGGA GCTCAAGGAG 
AACCTGGACC GGAGGGAGCG CGTGGTGCTG GATATCCTGG CCAACTACCT GTCCGCCGAG 
CAGCTGCAGG ACTACCAGCA CTTCGTGAAG ATGAAGTCCA CACTCCTCAT TGAGCAGCGG 
AAGCTGGATG ACAAGATAAA GCTGGGCCAG GAGCAGGTCA GGTGCCTGCT GGAGTCGCTT 
CCCTCGGACT TCAGGCCCAA GGCAGGGGCC ATCTCCCTCC CTCCAGCCCT CACCGGCCAT 
GGGACTCCTG GGGGGACATC TGTATTCGGT GGTGTTTTCC CAACGTTGAC CTCTCCTCTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056571.2
CDS	233..6175
Translation	MKTPENLEEPSATPNPSRTPTERFVYLEALLEGGAPWGFTLKGGLERGEPLIISKIEEGGKADSVSSGLQAGDEVIHINEVALSSSRREAVSLVKGSYKTLRLVVRRDVCAAPGHADPGTSKSLSSELLTCSPQHRKATWSGGVKLRLKQRCSEPATRPHSWHTTKFGETQPDVSMMQISQGTMGPPWHQSYHSSSSTSDLSNYDHAYLRRSPDQCSSQGSMESLEPSGGYPPCHLLSPAKSTSSIDQLGHLHNKRDSAYSSFSTSSSIFEYPPPGGSARERSGSMDVISARGGLLEGMRQADIRYVKTVYDTRRGVSSEYEVNPSALLLQGRDAHASADSQGCAKWHSIPRGKGTPSPSWSQQCSGSLETATDNLPQKAGAPLPPTRSDSYAAFRHRERPSSWSSLDQKRFCRPQTNSSGSQKTPFAEDQLHTVPERSPENSPPVKSKHNYTQKAQPGQPLLPTGIYPVPSPEPHFAQVPQPSVSSNGTVYPALVKESGYTAAQGTCNKMATLDENGNQNEASRPGFAFCQPLEHNSVTPVEKRPEPTAKYIYKVHFSSVPENEDSSLKRHITPPHGHSPYPSERKNIHGGSRACSNHHSLSSPQAQALHVGDDRRPSRLSQPWEGDFQEDHNANLRQKVEREGQGQGLSGNSGRTRSAFSSLQNIPESLRRQSNVELGEAQEVHPGGRSKVEDPGRKAGASDIRGYLDRSVSYPRPEGKMNAVDSVHSADSRYEESPAPALPQTSGASQRRLSSSSSAAPQYRKPHCSVLEKVSRIEEREQGRHRPLSVGSSAYGPGYRPGRTGPTPSTSSSDLDDPKAGSVHFSESTEHLRNGEQNPPNGEAKQEEASRPQCSHLIRRAPADGRGPPARGGEPSRPEARLLRSQSTFQLYSEAEREASWSEDRPGTPESPLLDAPFSRAYRNSIKDAQSRVLGATSFRRRDLEPGTPATSRPWRPRPASAHVGMRSPEAAVPSSSPHTPRERHSVTPAAPQAARRGPRRRLTVEQKKRSYSEPEKMNEVGVSEEAEPTPCGPPRPAQPRFSESTVADRRRIFERDGKACSTLSLSGPELKQFQQSALADYIQRKTGKRPTGAACTPEAGLRERAQSAYLQAGPAAPDGPGLASACSLSSLREPEALPRKEHTHPSAADGPQAPRDRSSSFASGRLVGERRRWDPQVPRQLLSGANCEPRGVQRMDGAPGGPPSWGMVAGKAGKSKSAEDLLERSDTLAVPVHVRSRSSPTSDKKGQDVLLREGSNFGFVKDPCCLAGPGPRSLSCSDKGQNELALPLHHPTPCWNGSGCKATVASSAPPESSGAADHLKQRRAPGPRPLSAGMHGHFPDARAASLSSPLPSPVPSSYRSQLAMDQQTGQQPPSSPASAVTQPTSPRSPELSSPAYGLGEGMWKRTSLPQRPPPPWVKWAHAVREDGLAEDTLAPEFANLKHYRNQPSRPSSCSTSDPDTPGRISLRISESALQPSPPPRGDYDDEVFMKDLHPKVTSSPTFEALPPPPPPSPPSEEPLVNGTDDFPPPPPPQALCEVLLDGEASTEAGSGPCRIPRVMVTREGHVPGAAHSEGSQIMTATPPQTSAKGSEAESNTPSSASAQPQLNGSPGKQLCPSQTRNLTYEPVERTQDLGKKTHAEPQKTSEDIRTEALAKEIVHQDKSLADILDPDSRMKTTMDLMEGLFPGDASVLMDSGAKRKALDITARRAGCEAKASDHKEAVSVLVNCPAYYSVSAAKAELLNKIKDMPEELQEEEGQEDVNEKKAELIGSLTHKLESLQEAKGSLLTDIKLNNALGEEVEALISELCKPNEFDKYKMFIGDLDKVVNLLLSLSGRLARVENVLRGLGEDASKEERSSLNEKRKVLAGQHEDARELKENLDRRERVVLDILANYLSAEQLQDYQHFVKMKSTLLIEQRKLDDKIKLGQEQVRCLLESLPSDFRPKAGAISLPPALTGHGTPGGTSVFGGVFPTLTSPL

Target ORF information:

RefSeq Version	NM_015756.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus shroom family member 3 (Shroom3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015756.2

ORF Insert Sequence:

ATGAAGACTC CGGAGAACTT GGAGGAGCCT AGCGCCACGC CAAACCCCAG TAGGACCCCC 
ACGGAGAGAT TCGTTTATCT GGAAGCGCTC CTAGAGGGAG GGGCTCCCTG GGGCTTCACC 
CTGAAAGGTG GCCTGGAGCG TGGAGAACCG TTAATCATTT CCAAGATTGA AGAAGGGGGC 
AAAGCCGACT CAGTGAGCTC CGGACTGCAA GCTGGGGACG AAGTCATACA CATCAATGAG 
GTGGCGCTGA GCAGTTCCAG GAGAGAGGCT GTCTCCCTAG TGAAAGGGTC CTACAAGACC 
CTGCGGCTGG TGGTGCGCAG AGACGTGTGT GCGGCCCCGG GCCATGCTGA TCCTGGTACC 
TCTAAGTCCC TCAGCTCAGA GCTGCTCACC TGCAGTCCCC AGCACCGGAA AGCAACATGG 
TCAGGAGGGG TGAAGCTTCG GCTAAAGCAG AGGTGCAGTG AGCCTGCCAC TCGCCCTCAC 
TCGTGGCATA CCACCAAATT TGGGGAGACA CAGCCTGATG TCAGCATGAT GCAGATATCT 
CAGGGCACAA TGGGCCCTCC TTGGCACCAG AGCTACCATT CCAGCTCTTC GACCAGTGAC 
CTCTCCAACT ACGACCATGC TTATCTGAGG CGGAGCCCTG ACCAGTGCAG CTCCCAGGGG 
AGCATGGAAA GCCTGGAGCC CAGTGGCGGA TACCCACCCT GCCATCTCCT TTCTCCTGCC 
AAGTCCACGA GCAGCATCGA CCAGCTAGGC CACCTTCATA ACAAAAGGGA CTCCGCATAT 
AGCTCCTTCT CCACCAGTTC GAGCATCTTC GAGTACCCAC CCCCTGGTGG CTCTGCCCGA 
GAACGTTCGG GTTCCATGGA CGTGATTTCT GCCCGGGGTG GCCTCCTAGA AGGGATGAGG 
CAGGCAGACA TCCGCTACGT CAAGACGGTC TATGATACCC GGAGAGGAGT ATCTTCAGAG 
TATGAGGTGA ACCCTTCAGC CTTGCTCCTT CAAGGTAGGG ATGCCCATGC CTCAGCCGAC 
AGTCAGGGCT GTGCTAAATG GCACAGCATT CCTCGGGGCA AGGGAACGCC TTCCCCGTCT 
TGGAGCCAGC AGTGTTCTGG TTCCCTGGAG ACTGCTACTG ACAACCTGCC CCAGAAGGCA 
GGCGCACCCC TGCCCCCCAC CCGCAGTGAT AGCTATGCAG CCTTCCGCCA TCGCGAGCGT 
CCCAGCTCCT GGTCCAGCCT TGATCAGAAA AGGTTCTGCC GGCCTCAGAC AAACTCTTCA 
GGCTCCCAGA AAACCCCATT CGCTGAGGAC CAGCTGCATA CAGTCCCAGA GAGGAGTCCA 
GAGAACAGCC CCCCAGTGAA ATCTAAGCAT AATTATACCC AGAAGGCGCA GCCTGGCCAA 
CCTCTGCTGC CCACTGGCAT CTATCCGGTA CCTTCTCCGG AGCCTCACTT TGCCCAGGTG 
CCCCAGCCCT CTGTGAGTAG TAATGGCACC GTCTACCCTG CGCTAGTCAA AGAGAGTGGA 
TACACAGCTG CTCAGGGAAC CTGTAACAAG ATGGCTACCT TAGATGAGAA CGGGAATCAA 
AATGAAGCTA GCAGGCCTGG GTTTGCCTTC TGTCAGCCCC TAGAACACAA CTCGGTGACT 
CCGGTGGAGA AAAGGCCAGA ACCCACGGCC AAATACATCT ACAAAGTCCA TTTTTCTTCA 
GTGCCTGAAA ATGAAGACTC CTCCCTAAAG AGGCACATCA CACCTCCCCA CGGCCATAGT 
CCATATCCCA GCGAGAGAAA GAACATCCAC GGTGGCAGCA GAGCGTGTTC CAATCACCAC 
AGTCTCTCGT CCCCCCAGGC CCAGGCCCTG CACGTGGGTG ATGACAGGAG GCCTTCCAGG 
CTCTCTCAGC CCTGGGAGGG TGACTTTCAG GAAGATCACA ATGCCAACCT CAGGCAGAAA 
GTGGAGAGGG AGGGCCAAGG CCAGGGCCTG TCGGGCAACT CTGGCAGGAC CAGATCGGCC 
TTCTCATCTC TGCAGAATAT TCCAGAGAGT CTGAGAAGAC AAAGCAACGT GGAGCTTGGA 
GAAGCCCAGG AGGTTCACCC CGGGGGCAGG TCCAAGGTGG AGGACCCTGG AAGGAAAGCA 
GGGGCTTCAG ACATCAGGGG CTACCTGGAC AGATCTGTTT CCTATCCGAG GCCCGAGGGG 
AAAATGAACG CCGTAGACTC CGTCCACAGT GCAGACTCCA GGTATGAGGA GTCCCCGGCC 
CCAGCGCTGC CACAGACATC CGGTGCGAGC CAGCGGCGGC TCAGCTCCAG CAGCTCCGCC 
GCCCCGCAGT ACAGGAAGCC CCATTGCTCC GTGCTGGAGA AGGTTTCTAG AATAGAGGAA 
CGAGAGCAGG GGCGCCACAG ACCCCTGAGC GTGGGCAGCT CTGCATACGG CCCCGGCTAC 
AGGCCTGGGA GGACCGGCCC GACCCCCAGT ACTTCCAGCA GTGACCTCGA CGATCCAAAA 
GCCGGCAGCG TCCATTTCTC GGAGTCCACT GAACACCTGC GCAATGGGGA GCAGAACCCC 
CCAAACGGGG AGGCTAAGCA GGAAGAGGCT TCCCGGCCCC AGTGCAGCCA TTTGATCCGG 
AGGGCCCCTG CGGACGGCCG TGGACCTCCT GCCCGAGGCG GCGAGCCCTC AAGGCCGGAG 
GCCCGCCTGC TCCGCAGCCA GAGCACCTTT CAGCTCTACA GTGAGGCGGA GAGGGAAGCC 
TCGTGGTCAG AGGACAGGCC CGGTACCCCT GAGTCGCCCC TGCTCGATGC GCCTTTCAGC 
CGCGCCTACA GAAACAGCAT CAAGGATGCC CAGTCCCGCG TCCTGGGGGC CACGTCGTTT 
CGTCGCCGAG ACCTGGAACC GGGCACCCCC GCCACCTCGA GGCCCTGGCG CCCGCGACCC 
GCCTCCGCCC ACGTGGGGAT GCGGAGCCCC GAGGCCGCGG TGCCCTCGTC CTCCCCGCAC 
ACCCCTCGGG AGCGACACAG CGTGACCCCG GCCGCGCCCC AGGCTGCCCG CCGAGGCCCA 
CGCCGGCGCC TCACTGTCGA GCAGAAGAAG CGCTCCTACT CGGAGCCCGA GAAGATGAAC 
GAGGTAGGGG TCTCGGAGGA GGCCGAGCCC ACGCCCTGCG GCCCGCCGAG GCCCGCGCAG 
CCGCGCTTCT CGGAGAGCAC CGTGGCCGAC CGGCGCCGCA TCTTCGAGCG CGACGGCAAG 
GCCTGCTCCA CGCTCAGCCT GTCGGGCCCC GAGCTCAAGC AGTTCCAGCA GAGCGCCCTG 
GCCGACTACA TCCAGCGTAA GACGGGCAAG CGGCCCACAG GAGCCGCCTG CACACCGGAG 
GCCGGGCTGC GCGAGCGCGC ACAGAGCGCC TACCTCCAGG CCGGCCCCGC GGCGCCCGAT 
GGCCCCGGCC TGGCCTCTGC CTGTAGCCTG AGCTCCCTGC GAGAGCCCGA GGCGCTGCCC 
CGCAAGGAGC ACACTCATCC GTCGGCCGCG GACGGCCCGC AGGCGCCCCG TGATCGCAGC 
AGCTCCTTCG CCAGCGGACG CCTAGTCGGG GAGCGACGTC GCTGGGACCC TCAGGTCCCC 
AGGCAGCTGC TCAGCGGAGC GAACTGCGAA CCGAGGGGCG TCCAGAGGAT GGACGGGGCC 
CCGGGCGGGC CGCCTTCCTG GGGCATGGTG GCCGGGAAGG CCGGGAAGTC CAAGTCAGCC 
GAGGATCTGC TGGAGCGCTC CGACACACTG GCGGTCCCTG TGCATGTGAG GTCCAGGTCG 
TCTCCCACCT CCGACAAGAA AGGTCAGGAT GTGCTCCTGA GGGAAGGCAG CAACTTTGGT 
TTTGTAAAGG ATCCATGCTG TTTGGCTGGC CCTGGACCTA GGTCTCTCAG CTGCTCAGAC 
AAAGGCCAGA ATGAGCTGGC ATTGCCCCTG CACCACCCTA CGCCCTGCTG GAACGGCTCA 
GGCTGCAAAG CCACTGTGGC TTCCTCAGCT CCTCCCGAAT CTTCTGGAGC CGCGGATCAT 
CTGAAGCAAC GTAGAGCGCC TGGTCCTAGG CCACTCTCAG CAGGAATGCA CGGCCACTTC 
CCAGACGCCA GAGCTGCCTC ACTCAGCTCC CCTCTGCCAT CACCTGTCCC CTCCAGTTAC 
CGGTCACAGC TTGCCATGGA TCAGCAGACT GGGCAACAGC CTCCCTCATC CCCTGCCTCT 
GCAGTGACCC AGCCTACCAG TCCAAGAAGC CCTGAGCTCA GCAGCCCAGC ATATGGGCTA 
GGGGAGGGGA TGTGGAAGAG GACATCCCTG CCTCAGCGAC CCCCTCCCCC TTGGGTGAAA 
TGGGCCCACG CGGTCAGAGA GGACGGCCTT GCTGAGGACA CCTTGGCGCC TGAGTTTGCG 
AACCTGAAGC ACTATAGAAA CCAGCCGAGT CGTCCGAGTT CCTGCAGCAC TTCTGACCCA 
GACACTCCAG GAAGGATCTC GCTCCGAATA TCGGAGTCAG CCCTTCAACC TTCCCCACCG 
CCCCGGGGGG ACTATGATGA CGAAGTGTTC ATGAAGGACT TGCACCCCAA GGTTACTTCA 
AGCCCCACAT TTGAAGCTCT TCCCCCACCC CCGCCACCTT CGCCACCGAG CGAGGAACCT 
CTAGTGAATG GCACAGATGA CTTTCCTCCG CCTCCTCCTC CCCAAGCCCT GTGTGAGGTA 
CTGCTGGATG GTGAGGCATC CACGGAAGCT GGCAGCGGCC CCTGCAGAAT TCCCAGGGTG 
ATGGTCACAA GGGAAGGGCA CGTGCCTGGG GCAGCCCATT CCGAAGGTAG TCAGATAATG 
ACTGCCACAC CACCCCAAAC CTCGGCCAAA GGTTCAGAAG CCGAGTCCAA CACGCCTTCC 
AGCGCGAGCG CCCAGCCTCA GCTCAACGGT TCTCCTGGCA AGCAGCTCTG TCCCAGCCAG 
ACCCGAAACC TCACTTATGA ACCTGTCGAG AGAACTCAGG ATCTAGGAAA GAAAACTCAC 
GCCGAGCCTC AGAAGACCTC AGAAGATATC CGGACAGAGG CTCTAGCCAA GGAAATTGTC 
CACCAAGACA AGTCTCTGGC AGACATTTTA GATCCTGACT CCAGGATGAA GACAACGATG 
GACCTGATGG AGGGTTTATT CCCTGGAGAC GCCAGTGTGC TGATGGATAG TGGCGCAAAG 
AGGAAAGCCT TAGACATCAC CGCCAGGCGT GCGGGATGTG AAGCCAAGGC GAGCGACCAC 
AAGGAGGCAG TGAGTGTGCT GGTCAACTGT CCTGCCTACT ACAGTGTCTC TGCTGCCAAG 
GCGGAGCTGC TCAACAAAAT CAAAGACATG CCGGAGGAGC TGCAGGAGGA GGAGGGGCAG 
GAGGATGTCA ATGAGAAAAA GGCTGAGCTT ATCGGAAGCC TTACCCACAA GCTGGAGAGC 
CTCCAGGAAG CCAAGGGGAG CCTGCTCACG GACATCAAGC TGAACAATGC CCTGGGAGAG 
GAGGTGGAGG CTCTGATCAG TGAGCTCTGC AAACCCAACG AGTTTGACAA GTACAAGATG 
TTCATAGGGG ACCTGGACAA GGTGGTCAAC CTGCTGCTGT CCCTGTCTGG ACGCCTGGCC 
CGCGTGGAGA ACGTCCTCAG AGGCCTTGGT GAAGACGCCA GCAAAGAGGA AAGGAGTTCT 
CTGAACGAGA AAAGGAAGGT TCTGGCTGGC CAGCATGAGG ATGCACGGGA GCTCAAGGAG 
AACCTGGACC GGAGGGAGCG CGTGGTGCTG GATATCCTGG CCAACTACCT GTCCGCCGAG 
CAGCTGCAGG ACTACCAGCA CTTCGTGAAG ATGAAGTCCA CACTCCTCAT TGAGCAGCGG 
AAGCTGGATG ACAAGATAAA GCTGGGCCAG GAGCAGGTCA GGTGCCTGCT GGAGTCGCTT 
CCCTCGGACT TCAGGCCCAA GGCAGGGGCC ATCTCCCTCC CTCCAGCCCT CACCGGCCAT 
GGGACTCCTG GGGGGACATC TGTATTCGGT GGTGTTTTCC CAACGTTGAC CTCTCCTCTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Shroom3 ( XM_006534968.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006535031.1 Mus musculus shroom family member 3 (Shroom3), transcript variant X2, mRNA.

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CloneID	OMu01300
Clone ID Related Accession (Same CDS sequence)	XM_006534968.2 , NM_001077595.2 , NM_001077596.2 , XM_006534968.3
Accession Version	XM_006534968.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5418bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein Shroom3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006534968.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCAGA TATCTCAGGG CACAATGGGC CCTCCTTGGC ACCAGAGCTA CCATTCCAGC 
TCTTCGACCA GTGACCTCTC CAACTACGAC CATGCTTATC TGAGGCGGAG CCCTGACCAG 
TGCAGCTCCC AGGGGAGCAT GGAAAGCCTG GAGCCCAGTG GCGGATACCC ACCCTGCCAT 
CTCCTTTCTC CTGCCAAGTC CACGAGCAGC ATCGACCAGC TAGGCCACCT TCATAACAAA 
AGGGACTCCG CATATAGCTC CTTCTCCACC AGTTCGAGCA TCTTCGAGTA CCCACCCCCT 
GGTGGCTCTG CCCGAGAACG TTCGGGTTCC ATGGACGTGA TTTCTGCCCG GGGTGGCCTC 
CTAGAAGGGA TGAGGCAGGC AGACATCCGC TACGTCAAGA CGGTCTATGA TACCCGGAGA 
GGAGTATCTT CAGAGTATGA GGTGAACCCT TCAGCCTTGC TCCTTCAAGG TAGGGATGCC 
CATGCCTCAG CCGACAGTCA GGGCTGTGCT AAATGGCACA GCATTCCTCG GGGCAAGGGA 
ACGCCTTCCC CGTCTTGGAG CCAGCAGTGT TCTGGTTCCC TGGAGACTGC TACTGACAAC 
CTGCCCCAGA AGGCAGGCGC ACCCCTGCCC CCCACCCGCA GTGATAGCTA TGCAGCCTTC 
CGCCATCGCG AGCGTCCCAG CTCCTGGTCC AGCCTTGATC AGAAAAGGTT CTGCCGGCCT 
CAGACAAACT CTTCAGGCTC CCAGAAAACC CCATTCGCTG AGGACCAGCT GCATACAGTC 
CCAGAGAGGA GTCCAGAGAA CAGCCCCCCA GTGAAATCTA AGCATAATTA TACCCAGAAG 
GCGCAGCCTG GCCAACCTCT GCTGCCCACT GGCATCTATC CGGTACCTTC TCCGGAGCCT 
CACTTTGCCC AGGTGCCCCA GCCCTCTGTG AGTAGTAATG GCACCGTCTA CCCTGCGCTA 
GTCAAAGAGA GTGGATACAC AGCTGCTCAG GGAACCTGTA ACAAGATGGC TACCTTAGAT 
GAGAACGGGA ATCAAAATGA AGCTAGCAGG CCTGGGTTTG CCTTCTGTCA GCCCCTAGAA 
CACAACTCGG TGACTCCGGT GGAGAAAAGG CCAGAACCCA CGGCCAAATA CATCTACAAA 
GTCCATTTTT CTTCAGTGCC TGAAAATGAA GACTCCTCCC TAAAGAGGCA CATCACACCT 
CCCCACGGCC ATAGTCCATA TCCCAGCGAG AGAAAGAACA TCCACGGTGG CAGCAGAGCG 
TGTTCCAATC ACCACAGTCT CTCGTCCCCC CAGGCCCAGG CCCTGCACGT GGGTGATGAC 
AGGAGGCCTT CCAGGCTCTC TCAGCCCTGG GAGGGTGACT TTCAGGAAGA TCACAATGCC 
AACCTCAGGC AGAAAGTGGA GAGGGAGGGC CAAGGCCAGG GCCTGTCGGG CAACTCTGGC 
AGGACCAGAT CGGCCTTCTC ATCTCTGCAG AATATTCCAG AGAGTCTGAG AAGACAAAGC 
AACGTGGAGC TTGGAGAAGC CCAGGAGGTT CACCCCGGGG GCAGGTCCAA GGTGGAGGAC 
CCTGGAAGGA AAGCAGGGGC TTCAGACATC AGGGGCTACC TGGACAGATC TGTTTCCTAT 
CCGAGGCCCG AGGGGAAAAT GAACGCCGTA GACTCCGTCC ACAGTGCAGA CTCCAGGTAT 
GAGGAGTCCC CGGCCCCAGC GCTGCCACAG ACATCCGGTG CGAGCCAGCG GCGGCTCAGC 
TCCAGCAGCT CCGCCGCCCC GCAGTACAGG AAGCCCCATT GCTCCGTGCT GGAGAAGGTT 
TCTAGAATAG AGGAACGAGA GCAGGGGCGC CACAGACCCC TGAGCGTGGG CAGCTCTGCA 
TACGGCCCCG GCTACAGGCC TGGGAGGACC GGCCCGACCC CCAGTACTTC CAGCAGTGAC 
CTCGACGATC CAAAAGCCGG CAGCGTCCAT TTCTCGGAGT CCACTGAACA CCTGCGCAAT 
GGGGAGCAGA ACCCCCCAAA CGGGGAGGCT AAGCAGGAAG AGGCTTCCCG GCCCCAGTGC 
AGCCATTTGA TCCGGAGGGC CCCTGCGGAC GGCCGTGGAC CTCCTGCCCG AGGCGGCGAG 
CCCTCAAGGC CGGAGGCCCG CCTGCTCCGC AGCCAGAGCA CCTTTCAGCT CTACAGTGAG 
GCGGAGAGGG AAGCCTCGTG GTCAGAGGAC AGGCCCGGTA CCCCTGAGTC GCCCCTGCTC 
GATGCGCCTT TCAGCCGCGC CTACAGAAAC AGCATCAAGG ATGCCCAGTC CCGCGTCCTG 
GGGGCCACGT CGTTTCGTCG CCGAGACCTG GAACCGGGCA CCCCCGCCAC CTCGAGGCCC 
TGGCGCCCGC GACCCGCCTC CGCCCACGTG GGGATGCGGA GCCCCGAGGC CGCGGTGCCC 
TCGTCCTCCC CGCACACCCC TCGGGAGCGA CACAGCGTGA CCCCGGCCGC GCCCCAGGCT 
GCCCGCCGAG GCCCACGCCG GCGCCTCACT GTCGAGCAGA AGAAGCGCTC CTACTCGGAG 
CCCGAGAAGA TGAACGAGGT AGGGGTCTCG GAGGAGGCCG AGCCCACGCC CTGCGGCCCG 
CCGAGGCCCG CGCAGCCGCG CTTCTCGGAG AGCACCGTGG CCGACCGGCG CCGCATCTTC 
GAGCGCGACG GCAAGGCCTG CTCCACGCTC AGCCTGTCGG GCCCCGAGCT CAAGCAGTTC 
CAGCAGAGCG CCCTGGCCGA CTACATCCAG CGTAAGACGG GCAAGCGGCC CACAGGAGCC 
GCCTGCACAC CGGAGGCCGG GCTGCGCGAG CGCGCACAGA GCGCCTACCT CCAGGCCGGC 
CCCGCGGCGC CCGATGGCCC CGGCCTGGCC TCTGCCTGTA GCCTGAGCTC CCTGCGAGAG 
CCCGAGGCGC TGCCCCGCAA GGAGCACACT CATCCGTCGG CCGCGGACGG CCCGCAGGCG 
CCCCGTGATC GCAGCAGCTC CTTCGCCAGC GGACGCCTAG TCGGGGAGCG ACGTCGCTGG 
GACCCTCAGG TCCCCAGGCA GCTGCTCAGC GGAGCGAACT GCGAACCGAG GGGCGTCCAG 
AGGATGGACG GGGCCCCGGG CGGGCCGCCT TCCTGGGGCA TGGTGGCCGG GAAGGCCGGG 
AAGTCCAAGT CAGCCGAGGA TCTGCTGGAG CGCTCCGACA CACTGGCGGT CCCTGTGCAT 
GTGAGGTCCA GGTCGTCTCC CACCTCCGAC AAGAAAGGTC AGGATGTGCT CCTGAGGGAA 
GGCAGCAACT TTGGTTTTGT AAAGGATCCA TGCTGTTTGG CTGGCCCTGG ACCTAGGTCT 
CTCAGCTGCT CAGACAAAGG CCAGAATGAG CTGGCATTGC CCCTGCACCA CCCTACGCCC 
TGCTGGAACG GCTCAGGCTG CAAAGCCACT GTGGCTTCCT CAGCTCCTCC CGAATCTTCT 
GGAGCCGCGG ATCATCTGAA GCAACGTAGA GCGCCTGGTC CTAGGCCACT CTCAGCAGGA 
ATGCACGGCC ACTTCCCAGA CGCCAGAGCT GCCTCACTCA GCTCCCCTCT GCCATCACCT 
GTCCCCTCCA GTTACCGGTC ACAGCTTGCC ATGGATCAGC AGACTGGGCA ACAGCCTCCC 
TCATCCCCTG CCTCTGCAGT GACCCAGCCT ACCAGTCCAA GAAGCCCTGA GCTCAGCAGC 
CCAGCATATG GGCTAGGGGA GGGGATGTGG AAGAGGACAT CCCTGCCTCA GCGACCCCCT 
CCCCCTTGGG TGAAATGGGC CCACGCGGTC AGAGAGGACG GCCTTGCTGA GGACACCTTG 
GCGCCTGAGT TTGCGAACCT GAAGCACTAT AGAAACCAGC CGAGTCGTCC GAGTTCCTGC 
AGCACTTCTG ACCCAGACAC TCCAGGAAGG ATCTCGCTCC GAATATCGGA GTCAGCCCTT 
CAACCTTCCC CACCGCCCCG GGGGGACTAT GATGACGAAG TGTTCATGAA GGACTTGCAC 
CCCAAGGTTA CTTCAAGCCC CACATTTGAA GCTCTTCCCC CACCCCCGCC ACCTTCGCCA 
CCGAGCGAGG AACCTCTAGT GAATGGCACA GATGACTTTC CTCCGCCTCC TCCTCCCCAA 
GCCCTGTGTG AGGTACTGCT GGATGGTGAG GCATCCACGG AAGCTGGCAG CGGCCCCTGC 
AGAATTCCCA GGGTGATGGT CACAAGGGAA GGGCACGTGC CTGGGGCAGC CCATTCCGAA 
GGTAGTCAGA TAATGACTGC CACACCACCC CAAACCTCGG CCAAAGGTTC AGAAGCCGAG 
TCCAACACGC CTTCCAGCGC GAGCGCCCAG CCTCAGCTCA ACGGTTCTCC TGGCAAGCAG 
CTCTGTCCCA GCCAGACCCG AAACCTCACT TATGAACCTG TCGAGAGAAC TCAGGATCTA 
GGAAAGAAAA CTCACGCCGA GCCTCAGAAG ACCTCAGAAG ATATCCGGAC AGAGGCTCTA 
GCCAAGGAAA TTGTCCACCA AGACAAGTCT CTGGCAGACA TTTTAGATCC TGACTCCAGG 
ATGAAGACAA CGATGGACCT GATGGAGGGT TTATTCCCTG GAGACGCCAG TGTGCTGATG 
GATAGTGGCG CAAAGAGGAA AGCCTTAGAC ATCACCGCCA GGCGTGCGGG ATGTGAAGCC 
AAGGCGAGCG ACCACAAGGA GGCAGTGAGT GTGCTGGTCA ACTGTCCTGC CTACTACAGT 
GTCTCTGCTG CCAAGGCGGA GCTGCTCAAC AAAATCAAAG ACATGCCGGA GGAGCTGCAG 
GAGGAGGAGG GGCAGGAGGA TGTCAATGAG AAAAAGGCTG AGCTTATCGG AAGCCTTACC 
CACAAGCTGG AGAGCCTCCA GGAAGCCAAG GGGAGCCTGC TCACGGACAT CAAGCTGAAC 
AATGCCCTGG GAGAGGAGGT GGAGGCTCTG ATCAGTGAGC TCTGCAAACC CAACGAGTTT 
GACAAGTACA AGATGTTCAT AGGGGACCTG GACAAGGTGG TCAACCTGCT GCTGTCCCTG 
TCTGGACGCC TGGCCCGCGT GGAGAACGTC CTCAGAGGCC TTGGTGAAGA CGCCAGCAAA 
GAGGAAAGGA GTTCTCTGAA CGAGAAAAGG AAGGTTCTGG CTGGCCAGCA TGAGGATGCA 
CGGGAGCTCA AGGAGAACCT GGACCGGAGG GAGCGCGTGG TGCTGGATAT CCTGGCCAAC 
TACCTGTCCG CCGAGCAGCT GCAGGACTAC CAGCACTTCG TGAAGATGAA GTCCACACTC 
CTCATTGAGC AGCGGAAGCT GGATGACAAG ATAAAGCTGG GCCAGGAGCA GGTCAGGTGC 
CTGCTGGAGT CGCTTCCCTC GGACTTCAGG CCCAAGGCAG GGGCCATCTC CCTCCCTCCA 
GCCCTCACCG GCCATGGGAC TCCTGGGGGG ACATCTGTAT TCGGTGGTGT TTTCCCAACG 
TTGACCTCTC CTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006535031.1
CDS	148..5565
Translation	MMQISQGTMGPPWHQSYHSSSSTSDLSNYDHAYLRRSPDQCSSQGSMESLEPSGGYPPCHLLSPAKSTSSIDQLGHLHNKRDSAYSSFSTSSSIFEYPPPGGSARERSGSMDVISARGGLLEGMRQADIRYVKTVYDTRRGVSSEYEVNPSALLLQGRDAHASADSQGCAKWHSIPRGKGTPSPSWSQQCSGSLETATDNLPQKAGAPLPPTRSDSYAAFRHRERPSSWSSLDQKRFCRPQTNSSGSQKTPFAEDQLHTVPERSPENSPPVKSKHNYTQKAQPGQPLLPTGIYPVPSPEPHFAQVPQPSVSSNGTVYPALVKESGYTAAQGTCNKMATLDENGNQNEASRPGFAFCQPLEHNSVTPVEKRPEPTAKYIYKVHFSSVPENEDSSLKRHITPPHGHSPYPSERKNIHGGSRACSNHHSLSSPQAQALHVGDDRRPSRLSQPWEGDFQEDHNANLRQKVEREGQGQGLSGNSGRTRSAFSSLQNIPESLRRQSNVELGEAQEVHPGGRSKVEDPGRKAGASDIRGYLDRSVSYPRPEGKMNAVDSVHSADSRYEESPAPALPQTSGASQRRLSSSSSAAPQYRKPHCSVLEKVSRIEEREQGRHRPLSVGSSAYGPGYRPGRTGPTPSTSSSDLDDPKAGSVHFSESTEHLRNGEQNPPNGEAKQEEASRPQCSHLIRRAPADGRGPPARGGEPSRPEARLLRSQSTFQLYSEAEREASWSEDRPGTPESPLLDAPFSRAYRNSIKDAQSRVLGATSFRRRDLEPGTPATSRPWRPRPASAHVGMRSPEAAVPSSSPHTPRERHSVTPAAPQAARRGPRRRLTVEQKKRSYSEPEKMNEVGVSEEAEPTPCGPPRPAQPRFSESTVADRRRIFERDGKACSTLSLSGPELKQFQQSALADYIQRKTGKRPTGAACTPEAGLRERAQSAYLQAGPAAPDGPGLASACSLSSLREPEALPRKEHTHPSAADGPQAPRDRSSSFASGRLVGERRRWDPQVPRQLLSGANCEPRGVQRMDGAPGGPPSWGMVAGKAGKSKSAEDLLERSDTLAVPVHVRSRSSPTSDKKGQDVLLREGSNFGFVKDPCCLAGPGPRSLSCSDKGQNELALPLHHPTPCWNGSGCKATVASSAPPESSGAADHLKQRRAPGPRPLSAGMHGHFPDARAASLSSPLPSPVPSSYRSQLAMDQQTGQQPPSSPASAVTQPTSPRSPELSSPAYGLGEGMWKRTSLPQRPPPPWVKWAHAVREDGLAEDTLAPEFANLKHYRNQPSRPSSCSTSDPDTPGRISLRISESALQPSPPPRGDYDDEVFMKDLHPKVTSSPTFEALPPPPPPSPPSEEPLVNGTDDFPPPPPPQALCEVLLDGEASTEAGSGPCRIPRVMVTREGHVPGAAHSEGSQIMTATPPQTSAKGSEAESNTPSSASAQPQLNGSPGKQLCPSQTRNLTYEPVERTQDLGKKTHAEPQKTSEDIRTEALAKEIVHQDKSLADILDPDSRMKTTMDLMEGLFPGDASVLMDSGAKRKALDITARRAGCEAKASDHKEAVSVLVNCPAYYSVSAAKAELLNKIKDMPEELQEEEGQEDVNEKKAELIGSLTHKLESLQEAKGSLLTDIKLNNALGEEVEALISELCKPNEFDKYKMFIGDLDKVVNLLLSLSGRLARVENVLRGLGEDASKEERSSLNEKRKVLAGQHEDARELKENLDRRERVVLDILANYLSAEQLQDYQHFVKMKSTLLIEQRKLDDKIKLGQEQVRCLLESLPSDFRPKAGAISLPPALTGHGTPGGTSVFGGVFPTLTSPL

Target ORF information:

RefSeq Version	XM_006534968.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus shroom family member 3 (Shroom3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006534968.2

ORF Insert Sequence:

ATGATGCAGA TATCTCAGGG CACAATGGGC CCTCCTTGGC ACCAGAGCTA CCATTCCAGC 
TCTTCGACCA GTGACCTCTC CAACTACGAC CATGCTTATC TGAGGCGGAG CCCTGACCAG 
TGCAGCTCCC AGGGGAGCAT GGAAAGCCTG GAGCCCAGTG GCGGATACCC ACCCTGCCAT 
CTCCTTTCTC CTGCCAAGTC CACGAGCAGC ATCGACCAGC TAGGCCACCT TCATAACAAA 
AGGGACTCCG CATATAGCTC CTTCTCCACC AGTTCGAGCA TCTTCGAGTA CCCACCCCCT 
GGTGGCTCTG CCCGAGAACG TTCGGGTTCC ATGGACGTGA TTTCTGCCCG GGGTGGCCTC 
CTAGAAGGGA TGAGGCAGGC AGACATCCGC TACGTCAAGA CGGTCTATGA TACCCGGAGA 
GGAGTATCTT CAGAGTATGA GGTGAACCCT TCAGCCTTGC TCCTTCAAGG TAGGGATGCC 
CATGCCTCAG CCGACAGTCA GGGCTGTGCT AAATGGCACA GCATTCCTCG GGGCAAGGGA 
ACGCCTTCCC CGTCTTGGAG CCAGCAGTGT TCTGGTTCCC TGGAGACTGC TACTGACAAC 
CTGCCCCAGA AGGCAGGCGC ACCCCTGCCC CCCACCCGCA GTGATAGCTA TGCAGCCTTC 
CGCCATCGCG AGCGTCCCAG CTCCTGGTCC AGCCTTGATC AGAAAAGGTT CTGCCGGCCT 
CAGACAAACT CTTCAGGCTC CCAGAAAACC CCATTCGCTG AGGACCAGCT GCATACAGTC 
CCAGAGAGGA GTCCAGAGAA CAGCCCCCCA GTGAAATCTA AGCATAATTA TACCCAGAAG 
GCGCAGCCTG GCCAACCTCT GCTGCCCACT GGCATCTATC CGGTACCTTC TCCGGAGCCT 
CACTTTGCCC AGGTGCCCCA GCCCTCTGTG AGTAGTAATG GCACCGTCTA CCCTGCGCTA 
GTCAAAGAGA GTGGATACAC AGCTGCTCAG GGAACCTGTA ACAAGATGGC TACCTTAGAT 
GAGAACGGGA ATCAAAATGA AGCTAGCAGG CCTGGGTTTG CCTTCTGTCA GCCCCTAGAA 
CACAACTCGG TGACTCCGGT GGAGAAAAGG CCAGAACCCA CGGCCAAATA CATCTACAAA 
GTCCATTTTT CTTCAGTGCC TGAAAATGAA GACTCCTCCC TAAAGAGGCA CATCACACCT 
CCCCACGGCC ATAGTCCATA TCCCAGCGAG AGAAAGAACA TCCACGGTGG CAGCAGAGCG 
TGTTCCAATC ACCACAGTCT CTCGTCCCCC CAGGCCCAGG CCCTGCACGT GGGTGATGAC 
AGGAGGCCTT CCAGGCTCTC TCAGCCCTGG GAGGGTGACT TTCAGGAAGA TCACAATGCC 
AACCTCAGGC AGAAAGTGGA GAGGGAGGGC CAAGGCCAGG GCCTGTCGGG CAACTCTGGC 
AGGACCAGAT CGGCCTTCTC ATCTCTGCAG AATATTCCAG AGAGTCTGAG AAGACAAAGC 
AACGTGGAGC TTGGAGAAGC CCAGGAGGTT CACCCCGGGG GCAGGTCCAA GGTGGAGGAC 
CCTGGAAGGA AAGCAGGGGC TTCAGACATC AGGGGCTACC TGGACAGATC TGTTTCCTAT 
CCGAGGCCCG AGGGGAAAAT GAACGCCGTA GACTCCGTCC ACAGTGCAGA CTCCAGGTAT 
GAGGAGTCCC CGGCCCCAGC GCTGCCACAG ACATCCGGTG CGAGCCAGCG GCGGCTCAGC 
TCCAGCAGCT CCGCCGCCCC GCAGTACAGG AAGCCCCATT GCTCCGTGCT GGAGAAGGTT 
TCTAGAATAG AGGAACGAGA GCAGGGGCGC CACAGACCCC TGAGCGTGGG CAGCTCTGCA 
TACGGCCCCG GCTACAGGCC TGGGAGGACC GGCCCGACCC CCAGTACTTC CAGCAGTGAC 
CTCGACGATC CAAAAGCCGG CAGCGTCCAT TTCTCGGAGT CCACTGAACA CCTGCGCAAT 
GGGGAGCAGA ACCCCCCAAA CGGGGAGGCT AAGCAGGAAG AGGCTTCCCG GCCCCAGTGC 
AGCCATTTGA TCCGGAGGGC CCCTGCGGAC GGCCGTGGAC CTCCTGCCCG AGGCGGCGAG 
CCCTCAAGGC CGGAGGCCCG CCTGCTCCGC AGCCAGAGCA CCTTTCAGCT CTACAGTGAG 
GCGGAGAGGG AAGCCTCGTG GTCAGAGGAC AGGCCCGGTA CCCCTGAGTC GCCCCTGCTC 
GATGCGCCTT TCAGCCGCGC CTACAGAAAC AGCATCAAGG ATGCCCAGTC CCGCGTCCTG 
GGGGCCACGT CGTTTCGTCG CCGAGACCTG GAACCGGGCA CCCCCGCCAC CTCGAGGCCC 
TGGCGCCCGC GACCCGCCTC CGCCCACGTG GGGATGCGGA GCCCCGAGGC CGCGGTGCCC 
TCGTCCTCCC CGCACACCCC TCGGGAGCGA CACAGCGTGA CCCCGGCCGC GCCCCAGGCT 
GCCCGCCGAG GCCCACGCCG GCGCCTCACT GTCGAGCAGA AGAAGCGCTC CTACTCGGAG 
CCCGAGAAGA TGAACGAGGT AGGGGTCTCG GAGGAGGCCG AGCCCACGCC CTGCGGCCCG 
CCGAGGCCCG CGCAGCCGCG CTTCTCGGAG AGCACCGTGG CCGACCGGCG CCGCATCTTC 
GAGCGCGACG GCAAGGCCTG CTCCACGCTC AGCCTGTCGG GCCCCGAGCT CAAGCAGTTC 
CAGCAGAGCG CCCTGGCCGA CTACATCCAG CGTAAGACGG GCAAGCGGCC CACAGGAGCC 
GCCTGCACAC CGGAGGCCGG GCTGCGCGAG CGCGCACAGA GCGCCTACCT CCAGGCCGGC 
CCCGCGGCGC CCGATGGCCC CGGCCTGGCC TCTGCCTGTA GCCTGAGCTC CCTGCGAGAG 
CCCGAGGCGC TGCCCCGCAA GGAGCACACT CATCCGTCGG CCGCGGACGG CCCGCAGGCG 
CCCCGTGATC GCAGCAGCTC CTTCGCCAGC GGACGCCTAG TCGGGGAGCG ACGTCGCTGG 
GACCCTCAGG TCCCCAGGCA GCTGCTCAGC GGAGCGAACT GCGAACCGAG GGGCGTCCAG 
AGGATGGACG GGGCCCCGGG CGGGCCGCCT TCCTGGGGCA TGGTGGCCGG GAAGGCCGGG 
AAGTCCAAGT CAGCCGAGGA TCTGCTGGAG CGCTCCGACA CACTGGCGGT CCCTGTGCAT 
GTGAGGTCCA GGTCGTCTCC CACCTCCGAC AAGAAAGGTC AGGATGTGCT CCTGAGGGAA 
GGCAGCAACT TTGGTTTTGT AAAGGATCCA TGCTGTTTGG CTGGCCCTGG ACCTAGGTCT 
CTCAGCTGCT CAGACAAAGG CCAGAATGAG CTGGCATTGC CCCTGCACCA CCCTACGCCC 
TGCTGGAACG GCTCAGGCTG CAAAGCCACT GTGGCTTCCT CAGCTCCTCC CGAATCTTCT 
GGAGCCGCGG ATCATCTGAA GCAACGTAGA GCGCCTGGTC CTAGGCCACT CTCAGCAGGA 
ATGCACGGCC ACTTCCCAGA CGCCAGAGCT GCCTCACTCA GCTCCCCTCT GCCATCACCT 
GTCCCCTCCA GTTACCGGTC ACAGCTTGCC ATGGATCAGC AGACTGGGCA ACAGCCTCCC 
TCATCCCCTG CCTCTGCAGT GACCCAGCCT ACCAGTCCAA GAAGCCCTGA GCTCAGCAGC 
CCAGCATATG GGCTAGGGGA GGGGATGTGG AAGAGGACAT CCCTGCCTCA GCGACCCCCT 
CCCCCTTGGG TGAAATGGGC CCACGCGGTC AGAGAGGACG GCCTTGCTGA GGACACCTTG 
GCGCCTGAGT TTGCGAACCT GAAGCACTAT AGAAACCAGC CGAGTCGTCC GAGTTCCTGC 
AGCACTTCTG ACCCAGACAC TCCAGGAAGG ATCTCGCTCC GAATATCGGA GTCAGCCCTT 
CAACCTTCCC CACCGCCCCG GGGGGACTAT GATGACGAAG TGTTCATGAA GGACTTGCAC 
CCCAAGGTTA CTTCAAGCCC CACATTTGAA GCTCTTCCCC CACCCCCGCC ACCTTCGCCA 
CCGAGCGAGG AACCTCTAGT GAATGGCACA GATGACTTTC CTCCGCCTCC TCCTCCCCAA 
GCCCTGTGTG AGGTACTGCT GGATGGTGAG GCATCCACGG AAGCTGGCAG CGGCCCCTGC 
AGAATTCCCA GGGTGATGGT CACAAGGGAA GGGCACGTGC CTGGGGCAGC CCATTCCGAA 
GGTAGTCAGA TAATGACTGC CACACCACCC CAAACCTCGG CCAAAGGTTC AGAAGCCGAG 
TCCAACACGC CTTCCAGCGC GAGCGCCCAG CCTCAGCTCA ACGGTTCTCC TGGCAAGCAG 
CTCTGTCCCA GCCAGACCCG AAACCTCACT TATGAACCTG TCGAGAGAAC TCAGGATCTA 
GGAAAGAAAA CTCACGCCGA GCCTCAGAAG ACCTCAGAAG ATATCCGGAC AGAGGCTCTA 
GCCAAGGAAA TTGTCCACCA AGACAAGTCT CTGGCAGACA TTTTAGATCC TGACTCCAGG 
ATGAAGACAA CGATGGACCT GATGGAGGGT TTATTCCCTG GAGACGCCAG TGTGCTGATG 
GATAGTGGCG CAAAGAGGAA AGCCTTAGAC ATCACCGCCA GGCGTGCGGG ATGTGAAGCC 
AAGGCGAGCG ACCACAAGGA GGCAGTGAGT GTGCTGGTCA ACTGTCCTGC CTACTACAGT 
GTCTCTGCTG CCAAGGCGGA GCTGCTCAAC AAAATCAAAG ACATGCCGGA GGAGCTGCAG 
GAGGAGGAGG GGCAGGAGGA TGTCAATGAG AAAAAGGCTG AGCTTATCGG AAGCCTTACC 
CACAAGCTGG AGAGCCTCCA GGAAGCCAAG GGGAGCCTGC TCACGGACAT CAAGCTGAAC 
AATGCCCTGG GAGAGGAGGT GGAGGCTCTG ATCAGTGAGC TCTGCAAACC CAACGAGTTT 
GACAAGTACA AGATGTTCAT AGGGGACCTG GACAAGGTGG TCAACCTGCT GCTGTCCCTG 
TCTGGACGCC TGGCCCGCGT GGAGAACGTC CTCAGAGGCC TTGGTGAAGA CGCCAGCAAA 
GAGGAAAGGA GTTCTCTGAA CGAGAAAAGG AAGGTTCTGG CTGGCCAGCA TGAGGATGCA 
CGGGAGCTCA AGGAGAACCT GGACCGGAGG GAGCGCGTGG TGCTGGATAT CCTGGCCAAC 
TACCTGTCCG CCGAGCAGCT GCAGGACTAC CAGCACTTCG TGAAGATGAA GTCCACACTC 
CTCATTGAGC AGCGGAAGCT GGATGACAAG ATAAAGCTGG GCCAGGAGCA GGTCAGGTGC 
CTGCTGGAGT CGCTTCCCTC GGACTTCAGG CCCAAGGCAG GGGCCATCTC CCTCCCTCCA 
GCCCTCACCG GCCATGGGAC TCCTGGGGGG ACATCTGTAT TCGGTGGTGT TTTCCCAACG 
TTGACCTCTC CTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Shroom3 ( XM_006534966.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006535029.1 Mus musculus shroom family member 3 (Shroom3), transcript variant X1, mRNA.

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CloneID	OMu35489
Clone ID Related Accession (Same CDS sequence)	XM_006534966.3 , XM_006534966.4
Accession Version	XM_006534966.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5700bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein Shroom3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006534966.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAGGA GCAAGGGCTG CCTGGCTCTC GTGCTTCCCA GATTTGGGAC CCAAAACTCA 
AGAAGGCAAC AGAAGCTAGA CGTGTGTGCG GCCCCGGGCC ATGCTGATCC TGGTACCTCT 
AAGTCCCTCA GCTCAGAGCT GCTCACCTGC AGTCCCCAGC ACCGGAAAGC AACATGGTCA 
GGAGGGGTGA AGCTTCGGCT AAAGCAGAGG TGCAGTGAGC CTGCCACTCG CCCTCACTCG 
TGGCATACCA CCAAATTTGG GGAGACACAG CCTGATGTCA GCATGATGCA GATATCTCAG 
GGCACAATGG GCCCTCCTTG GCACCAGAGC TACCATTCCA GCTCTTCGAC CAGTGACCTC 
TCCAACTACG ACCATGCTTA TCTGAGGCGG AGCCCTGACC AGTGCAGCTC CCAGGGGAGC 
ATGGAAAGCC TGGAGCCCAG TGGCGGATAC CCACCCTGCC ATCTCCTTTC TCCTGCCAAG 
TCCACGAGCA GCATCGACCA GCTAGGCCAC CTTCATAACA AAAGGGACTC CGCATATAGC 
TCCTTCTCCA CCAGTTCGAG CATCTTCGAG TACCCACCCC CTGGTGGCTC TGCCCGAGAA 
CGTTCGGGTT CCATGGACGT GATTTCTGCC CGGGGTGGCC TCCTAGAAGG GATGAGGCAG 
GCAGACATCC GCTACGTCAA GACGGTCTAT GATACCCGGA GAGGAGTATC TTCAGAGTAT 
GAGGTGAACC CTTCAGCCTT GCTCCTTCAA GGTAGGGATG CCCATGCCTC AGCCGACAGT 
CAGGGCTGTG CTAAATGGCA CAGCATTCCT CGGGGCAAGG GAACGCCTTC CCCGTCTTGG 
AGCCAGCAGT GTTCTGGTTC CCTGGAGACT GCTACTGACA ACCTGCCCCA GAAGGCAGGC 
GCACCCCTGC CCCCCACCCG CAGTGATAGC TATGCAGCCT TCCGCCATCG CGAGCGTCCC 
AGCTCCTGGT CCAGCCTTGA TCAGAAAAGG TTCTGCCGGC CTCAGACAAA CTCTTCAGGC 
TCCCAGAAAA CCCCATTCGC TGAGGACCAG CTGCATACAG TCCCAGAGAG GAGTCCAGAG 
AACAGCCCCC CAGTGAAATC TAAGCATAAT TATACCCAGA AGGCGCAGCC TGGCCAACCT 
CTGCTGCCCA CTGGCATCTA TCCGGTACCT TCTCCGGAGC CTCACTTTGC CCAGGTGCCC 
CAGCCCTCTG TGAGTAGTAA TGGCACCGTC TACCCTGCGC TAGTCAAAGA GAGTGGATAC 
ACAGCTGCTC AGGGAACCTG TAACAAGATG GCTACCTTAG ATGAGAACGG GAATCAAAAT 
GAAGCTAGCA GGCCTGGGTT TGCCTTCTGT CAGCCCCTAG AACACAACTC GGTGACTCCG 
GTGGAGAAAA GGCCAGAACC CACGGCCAAA TACATCTACA AAGTCCATTT TTCTTCAGTG 
CCTGAAAATG AAGACTCCTC CCTAAAGAGG CACATCACAC CTCCCCACGG CCATAGTCCA 
TATCCCAGCG AGAGAAAGAA CATCCACGGT GGCAGCAGAG CGTGTTCCAA TCACCACAGT 
CTCTCGTCCC CCCAGGCCCA GGCCCTGCAC GTGGGTGATG ACAGGAGGCC TTCCAGGCTC 
TCTCAGCCCT GGGAGGGTGA CTTTCAGGAA GATCACAATG CCAACCTCAG GCAGAAAGTG 
GAGAGGGAGG GCCAAGGCCA GGGCCTGTCG GGCAACTCTG GCAGGACCAG ATCGGCCTTC 
TCATCTCTGC AGAATATTCC AGAGAGTCTG AGAAGACAAA GCAACGTGGA GCTTGGAGAA 
GCCCAGGAGG TTCACCCCGG GGGCAGGTCC AAGGTGGAGG ACCCTGGAAG GAAAGCAGGG 
GCTTCAGACA TCAGGGGCTA CCTGGACAGA TCTGTTTCCT ATCCGAGGCC CGAGGGGAAA 
ATGAACGCCG TAGACTCCGT CCACAGTGCA GACTCCAGGT ATGAGGAGTC CCCGGCCCCA 
GCGCTGCCAC AGACATCCGG TGCGAGCCAG CGGCGGCTCA GCTCCAGCAG CTCCGCCGCC 
CCGCAGTACA GGAAGCCCCA TTGCTCCGTG CTGGAGAAGG TTTCTAGAAT AGAGGAACGA 
GAGCAGGGGC GCCACAGACC CCTGAGCGTG GGCAGCTCTG CATACGGCCC CGGCTACAGG 
CCTGGGAGGA CCGGCCCGAC CCCCAGTACT TCCAGCAGTG ACCTCGACGA TCCAAAAGCC 
GGCAGCGTCC ATTTCTCGGA GTCCACTGAA CACCTGCGCA ATGGGGAGCA GAACCCCCCA 
AACGGGGAGG CTAAGCAGGA AGAGGCTTCC CGGCCCCAGT GCAGCCATTT GATCCGGAGG 
GCCCCTGCGG ACGGCCGTGG ACCTCCTGCC CGAGGCGGCG AGCCCTCAAG GCCGGAGGCC 
CGCCTGCTCC GCAGCCAGAG CACCTTTCAG CTCTACAGTG AGGCGGAGAG GGAAGCCTCG 
TGGTCAGAGG ACAGGCCCGG TACCCCTGAG TCGCCCCTGC TCGATGCGCC TTTCAGCCGC 
GCCTACAGAA ACAGCATCAA GGATGCCCAG TCCCGCGTCC TGGGGGCCAC GTCGTTTCGT 
CGCCGAGACC TGGAACCGGG CACCCCCGCC ACCTCGAGGC CCTGGCGCCC GCGACCCGCC 
TCCGCCCACG TGGGGATGCG GAGCCCCGAG GCCGCGGTGC CCTCGTCCTC CCCGCACACC 
CCTCGGGAGC GACACAGCGT GACCCCGGCC GCGCCCCAGG CTGCCCGCCG AGGCCCACGC 
CGGCGCCTCA CTGTCGAGCA GAAGAAGCGC TCCTACTCGG AGCCCGAGAA GATGAACGAG 
GTAGGGGTCT CGGAGGAGGC CGAGCCCACG CCCTGCGGCC CGCCGAGGCC CGCGCAGCCG 
CGCTTCTCGG AGAGCACCGT GGCCGACCGG CGCCGCATCT TCGAGCGCGA CGGCAAGGCC 
TGCTCCACGC TCAGCCTGTC GGGCCCCGAG CTCAAGCAGT TCCAGCAGAG CGCCCTGGCC 
GACTACATCC AGCGTAAGAC GGGCAAGCGG CCCACAGGAG CCGCCTGCAC ACCGGAGGCC 
GGGCTGCGCG AGCGCGCACA GAGCGCCTAC CTCCAGGCCG GCCCCGCGGC GCCCGATGGC 
CCCGGCCTGG CCTCTGCCTG TAGCCTGAGC TCCCTGCGAG AGCCCGAGGC GCTGCCCCGC 
AAGGAGCACA CTCATCCGTC GGCCGCGGAC GGCCCGCAGG CGCCCCGTGA TCGCAGCAGC 
TCCTTCGCCA GCGGACGCCT AGTCGGGGAG CGACGTCGCT GGGACCCTCA GGTCCCCAGG 
CAGCTGCTCA GCGGAGCGAA CTGCGAACCG AGGGGCGTCC AGAGGATGGA CGGGGCCCCG 
GGCGGGCCGC CTTCCTGGGG CATGGTGGCC GGGAAGGCCG GGAAGTCCAA GTCAGCCGAG 
GATCTGCTGG AGCGCTCCGA CACACTGGCG GTCCCTGTGC ATGTGAGGTC CAGGTCGTCT 
CCCACCTCCG ACAAGAAAGG TCAGGATGTG CTCCTGAGGG AAGGCAGCAA CTTTGGTTTT 
GTAAAGGATC CATGCTGTTT GGCTGGCCCT GGACCTAGGT CTCTCAGCTG CTCAGACAAA 
GGCCAGAATG AGCTGGCATT GCCCCTGCAC CACCCTACGC CCTGCTGGAA CGGCTCAGGC 
TGCAAAGCCA CTGTGGCTTC CTCAGCTCCT CCCGAATCTT CTGGAGCCGC GGATCATCTG 
AAGCAACGTA GAGCGCCTGG TCCTAGGCCA CTCTCAGCAG GAATGCACGG CCACTTCCCA 
GACGCCAGAG CTGCCTCACT CAGCTCCCCT CTGCCATCAC CTGTCCCCTC CAGTTACCGG 
TCACAGCTTG CCATGGATCA GCAGACTGGG CAACAGCCTC CCTCATCCCC TGCCTCTGCA 
GTGACCCAGC CTACCAGTCC AAGAAGCCCT GAGCTCAGCA GCCCAGCATA TGGGCTAGGG 
GAGGGGATGT GGAAGAGGAC ATCCCTGCCT CAGCGACCCC CTCCCCCTTG GGTGAAATGG 
GCCCACGCGG TCAGAGAGGA CGGCCTTGCT GAGGACACCT TGGCGCCTGA GTTTGCGAAC 
CTGAAGCACT ATAGAAACCA GCCGAGTCGT CCGAGTTCCT GCAGCACTTC TGACCCAGAC 
ACTCCAGGAA GGATCTCGCT CCGAATATCG GAGTCAGCCC TTCAACCTTC CCCACCGCCC 
CGGGGGGACT ATGATGACGA AGTGTTCATG AAGGACTTGC ACCCCAAGGT TACTTCAAGC 
CCCACATTTG AAGCTCTTCC CCCACCCCCG CCACCTTCGC CACCGAGCGA GGAACCTCTA 
GTGAATGGCA CAGATGACTT TCCTCCGCCT CCTCCTCCCC AAGCCCTGTG TGAGGTACTG 
CTGGATGGTG AGGCATCCAC GGAAGCTGGC AGCGGCCCCT GCAGAATTCC CAGGGTGATG 
GTCACAAGGG AAGGGCACGT GCCTGGGGCA GCCCATTCCG AAGGTAGTCA GATAATGACT 
GCCACACCAC CCCAAACCTC GGCCAAAGGT TCAGAAGCCG AGTCCAACAC GCCTTCCAGC 
GCGAGCGCCC AGCCTCAGCT CAACGGTTCT CCTGGCAAGC AGCTCTGTCC CAGCCAGACC 
CGAAACCTCA CTTATGAACC TGTCGAGAGA ACTCAGGATC TAGGAAAGAA AACTCACGCC 
GAGCCTCAGA AGACCTCAGA AGATATCCGG ACAGAGGCTC TAGCCAAGGA AATTGTCCAC 
CAAGACAAGT CTCTGGCAGA CATTTTAGAT CCTGACTCCA GGATGAAGAC AACGATGGAC 
CTGATGGAGG GTTTATTCCC TGGAGACGCC AGTGTGCTGA TGGATAGTGG CGCAAAGAGG 
AAAGCCTTAG ACATCACCGC CAGGCGTGCG GGATGTGAAG CCAAGGCGAG CGACCACAAG 
GAGGCAGTGA GTGTGCTGGT CAACTGTCCT GCCTACTACA GTGTCTCTGC TGCCAAGGCG 
GAGCTGCTCA ACAAAATCAA AGACATGCCG GAGGAGCTGC AGGAGGAGGA GGGGCAGGAG 
GATGTCAATG AGAAAAAGGC TGAGCTTATC GGAAGCCTTA CCCACAAGCT GGAGAGCCTC 
CAGGAAGCCA AGGGGAGCCT GCTCACGGAC ATCAAGCTGA ACAATGCCCT GGGAGAGGAG 
GTGGAGGCTC TGATCAGTGA GCTCTGCAAA CCCAACGAGT TTGACAAGTA CAAGATGTTC 
ATAGGGGACC TGGACAAGGT GGTCAACCTG CTGCTGTCCC TGTCTGGACG CCTGGCCCGC 
GTGGAGAACG TCCTCAGAGG CCTTGGTGAA GACGCCAGCA AAGAGGAAAG GAGTTCTCTG 
AACGAGAAAA GGAAGGTTCT GGCTGGCCAG CATGAGGATG CACGGGAGCT CAAGGAGAAC 
CTGGACCGGA GGGAGCGCGT GGTGCTGGAT ATCCTGGCCA ACTACCTGTC CGCCGAGCAG 
CTGCAGGACT ACCAGCACTT CGTGAAGATG AAGTCCACAC TCCTCATTGA GCAGCGGAAG 
CTGGATGACA AGATAAAGCT GGGCCAGGAG CAGGTCAGGT GCCTGCTGGA GTCGCTTCCC 
TCGGACTTCA GGCCCAAGGC AGGGGCCATC TCCCTCCCTC CAGCCCTCAC CGGCCATGGG 
ACTCCTGGGG GGACATCTGT ATTCGGTGGT GTTTTCCCAA CGTTGACCTC TCCTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006535029.1
CDS	415..6114
Translation	MVRSKGCLALVLPRFGTQNSRRQQKLDVCAAPGHADPGTSKSLSSELLTCSPQHRKATWSGGVKLRLKQRCSEPATRPHSWHTTKFGETQPDVSMMQISQGTMGPPWHQSYHSSSSTSDLSNYDHAYLRRSPDQCSSQGSMESLEPSGGYPPCHLLSPAKSTSSIDQLGHLHNKRDSAYSSFSTSSSIFEYPPPGGSARERSGSMDVISARGGLLEGMRQADIRYVKTVYDTRRGVSSEYEVNPSALLLQGRDAHASADSQGCAKWHSIPRGKGTPSPSWSQQCSGSLETATDNLPQKAGAPLPPTRSDSYAAFRHRERPSSWSSLDQKRFCRPQTNSSGSQKTPFAEDQLHTVPERSPENSPPVKSKHNYTQKAQPGQPLLPTGIYPVPSPEPHFAQVPQPSVSSNGTVYPALVKESGYTAAQGTCNKMATLDENGNQNEASRPGFAFCQPLEHNSVTPVEKRPEPTAKYIYKVHFSSVPENEDSSLKRHITPPHGHSPYPSERKNIHGGSRACSNHHSLSSPQAQALHVGDDRRPSRLSQPWEGDFQEDHNANLRQKVEREGQGQGLSGNSGRTRSAFSSLQNIPESLRRQSNVELGEAQEVHPGGRSKVEDPGRKAGASDIRGYLDRSVSYPRPEGKMNAVDSVHSADSRYEESPAPALPQTSGASQRRLSSSSSAAPQYRKPHCSVLEKVSRIEEREQGRHRPLSVGSSAYGPGYRPGRTGPTPSTSSSDLDDPKAGSVHFSESTEHLRNGEQNPPNGEAKQEEASRPQCSHLIRRAPADGRGPPARGGEPSRPEARLLRSQSTFQLYSEAEREASWSEDRPGTPESPLLDAPFSRAYRNSIKDAQSRVLGATSFRRRDLEPGTPATSRPWRPRPASAHVGMRSPEAAVPSSSPHTPRERHSVTPAAPQAARRGPRRRLTVEQKKRSYSEPEKMNEVGVSEEAEPTPCGPPRPAQPRFSESTVADRRRIFERDGKACSTLSLSGPELKQFQQSALADYIQRKTGKRPTGAACTPEAGLRERAQSAYLQAGPAAPDGPGLASACSLSSLREPEALPRKEHTHPSAADGPQAPRDRSSSFASGRLVGERRRWDPQVPRQLLSGANCEPRGVQRMDGAPGGPPSWGMVAGKAGKSKSAEDLLERSDTLAVPVHVRSRSSPTSDKKGQDVLLREGSNFGFVKDPCCLAGPGPRSLSCSDKGQNELALPLHHPTPCWNGSGCKATVASSAPPESSGAADHLKQRRAPGPRPLSAGMHGHFPDARAASLSSPLPSPVPSSYRSQLAMDQQTGQQPPSSPASAVTQPTSPRSPELSSPAYGLGEGMWKRTSLPQRPPPPWVKWAHAVREDGLAEDTLAPEFANLKHYRNQPSRPSSCSTSDPDTPGRISLRISESALQPSPPPRGDYDDEVFMKDLHPKVTSSPTFEALPPPPPPSPPSEEPLVNGTDDFPPPPPPQALCEVLLDGEASTEAGSGPCRIPRVMVTREGHVPGAAHSEGSQIMTATPPQTSAKGSEAESNTPSSASAQPQLNGSPGKQLCPSQTRNLTYEPVERTQDLGKKTHAEPQKTSEDIRTEALAKEIVHQDKSLADILDPDSRMKTTMDLMEGLFPGDASVLMDSGAKRKALDITARRAGCEAKASDHKEAVSVLVNCPAYYSVSAAKAELLNKIKDMPEELQEEEGQEDVNEKKAELIGSLTHKLESLQEAKGSLLTDIKLNNALGEEVEALISELCKPNEFDKYKMFIGDLDKVVNLLLSLSGRLARVENVLRGLGEDASKEERSSLNEKRKVLAGQHEDARELKENLDRRERVVLDILANYLSAEQLQDYQHFVKMKSTLLIEQRKLDDKIKLGQEQVRCLLESLPSDFRPKAGAISLPPALTGHGTPGGTSVFGGVFPTLTSPL

Target ORF information:

RefSeq Version	XM_006534966.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus shroom family member 3 (Shroom3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006534966.3

ORF Insert Sequence:

ATGGTGAGGA GCAAGGGCTG CCTGGCTCTC GTGCTTCCCA GATTTGGGAC CCAAAACTCA 
AGAAGGCAAC AGAAGCTAGA CGTGTGTGCG GCCCCGGGCC ATGCTGATCC TGGTACCTCT 
AAGTCCCTCA GCTCAGAGCT GCTCACCTGC AGTCCCCAGC ACCGGAAAGC AACATGGTCA 
GGAGGGGTGA AGCTTCGGCT AAAGCAGAGG TGCAGTGAGC CTGCCACTCG CCCTCACTCG 
TGGCATACCA CCAAATTTGG GGAGACACAG CCTGATGTCA GCATGATGCA GATATCTCAG 
GGCACAATGG GCCCTCCTTG GCACCAGAGC TACCATTCCA GCTCTTCGAC CAGTGACCTC 
TCCAACTACG ACCATGCTTA TCTGAGGCGG AGCCCTGACC AGTGCAGCTC CCAGGGGAGC 
ATGGAAAGCC TGGAGCCCAG TGGCGGATAC CCACCCTGCC ATCTCCTTTC TCCTGCCAAG 
TCCACGAGCA GCATCGACCA GCTAGGCCAC CTTCATAACA AAAGGGACTC CGCATATAGC 
TCCTTCTCCA CCAGTTCGAG CATCTTCGAG TACCCACCCC CTGGTGGCTC TGCCCGAGAA 
CGTTCGGGTT CCATGGACGT GATTTCTGCC CGGGGTGGCC TCCTAGAAGG GATGAGGCAG 
GCAGACATCC GCTACGTCAA GACGGTCTAT GATACCCGGA GAGGAGTATC TTCAGAGTAT 
GAGGTGAACC CTTCAGCCTT GCTCCTTCAA GGTAGGGATG CCCATGCCTC AGCCGACAGT 
CAGGGCTGTG CTAAATGGCA CAGCATTCCT CGGGGCAAGG GAACGCCTTC CCCGTCTTGG 
AGCCAGCAGT GTTCTGGTTC CCTGGAGACT GCTACTGACA ACCTGCCCCA GAAGGCAGGC 
GCACCCCTGC CCCCCACCCG CAGTGATAGC TATGCAGCCT TCCGCCATCG CGAGCGTCCC 
AGCTCCTGGT CCAGCCTTGA TCAGAAAAGG TTCTGCCGGC CTCAGACAAA CTCTTCAGGC 
TCCCAGAAAA CCCCATTCGC TGAGGACCAG CTGCATACAG TCCCAGAGAG GAGTCCAGAG 
AACAGCCCCC CAGTGAAATC TAAGCATAAT TATACCCAGA AGGCGCAGCC TGGCCAACCT 
CTGCTGCCCA CTGGCATCTA TCCGGTACCT TCTCCGGAGC CTCACTTTGC CCAGGTGCCC 
CAGCCCTCTG TGAGTAGTAA TGGCACCGTC TACCCTGCGC TAGTCAAAGA GAGTGGATAC 
ACAGCTGCTC AGGGAACCTG TAACAAGATG GCTACCTTAG ATGAGAACGG GAATCAAAAT 
GAAGCTAGCA GGCCTGGGTT TGCCTTCTGT CAGCCCCTAG AACACAACTC GGTGACTCCG 
GTGGAGAAAA GGCCAGAACC CACGGCCAAA TACATCTACA AAGTCCATTT TTCTTCAGTG 
CCTGAAAATG AAGACTCCTC CCTAAAGAGG CACATCACAC CTCCCCACGG CCATAGTCCA 
TATCCCAGCG AGAGAAAGAA CATCCACGGT GGCAGCAGAG CGTGTTCCAA TCACCACAGT 
CTCTCGTCCC CCCAGGCCCA GGCCCTGCAC GTGGGTGATG ACAGGAGGCC TTCCAGGCTC 
TCTCAGCCCT GGGAGGGTGA CTTTCAGGAA GATCACAATG CCAACCTCAG GCAGAAAGTG 
GAGAGGGAGG GCCAAGGCCA GGGCCTGTCG GGCAACTCTG GCAGGACCAG ATCGGCCTTC 
TCATCTCTGC AGAATATTCC AGAGAGTCTG AGAAGACAAA GCAACGTGGA GCTTGGAGAA 
GCCCAGGAGG TTCACCCCGG GGGCAGGTCC AAGGTGGAGG ACCCTGGAAG GAAAGCAGGG 
GCTTCAGACA TCAGGGGCTA CCTGGACAGA TCTGTTTCCT ATCCGAGGCC CGAGGGGAAA 
ATGAACGCCG TAGACTCCGT CCACAGTGCA GACTCCAGGT ATGAGGAGTC CCCGGCCCCA 
GCGCTGCCAC AGACATCCGG TGCGAGCCAG CGGCGGCTCA GCTCCAGCAG CTCCGCCGCC 
CCGCAGTACA GGAAGCCCCA TTGCTCCGTG CTGGAGAAGG TTTCTAGAAT AGAGGAACGA 
GAGCAGGGGC GCCACAGACC CCTGAGCGTG GGCAGCTCTG CATACGGCCC CGGCTACAGG 
CCTGGGAGGA CCGGCCCGAC CCCCAGTACT TCCAGCAGTG ACCTCGACGA TCCAAAAGCC 
GGCAGCGTCC ATTTCTCGGA GTCCACTGAA CACCTGCGCA ATGGGGAGCA GAACCCCCCA 
AACGGGGAGG CTAAGCAGGA AGAGGCTTCC CGGCCCCAGT GCAGCCATTT GATCCGGAGG 
GCCCCTGCGG ACGGCCGTGG ACCTCCTGCC CGAGGCGGCG AGCCCTCAAG GCCGGAGGCC 
CGCCTGCTCC GCAGCCAGAG CACCTTTCAG CTCTACAGTG AGGCGGAGAG GGAAGCCTCG 
TGGTCAGAGG ACAGGCCCGG TACCCCTGAG TCGCCCCTGC TCGATGCGCC TTTCAGCCGC 
GCCTACAGAA ACAGCATCAA GGATGCCCAG TCCCGCGTCC TGGGGGCCAC GTCGTTTCGT 
CGCCGAGACC TGGAACCGGG CACCCCCGCC ACCTCGAGGC CCTGGCGCCC GCGACCCGCC 
TCCGCCCACG TGGGGATGCG GAGCCCCGAG GCCGCGGTGC CCTCGTCCTC CCCGCACACC 
CCTCGGGAGC GACACAGCGT GACCCCGGCC GCGCCCCAGG CTGCCCGCCG AGGCCCACGC 
CGGCGCCTCA CTGTCGAGCA GAAGAAGCGC TCCTACTCGG AGCCCGAGAA GATGAACGAG 
GTAGGGGTCT CGGAGGAGGC CGAGCCCACG CCCTGCGGCC CGCCGAGGCC CGCGCAGCCG 
CGCTTCTCGG AGAGCACCGT GGCCGACCGG CGCCGCATCT TCGAGCGCGA CGGCAAGGCC 
TGCTCCACGC TCAGCCTGTC GGGCCCCGAG CTCAAGCAGT TCCAGCAGAG CGCCCTGGCC 
GACTACATCC AGCGTAAGAC GGGCAAGCGG CCCACAGGAG CCGCCTGCAC ACCGGAGGCC 
GGGCTGCGCG AGCGCGCACA GAGCGCCTAC CTCCAGGCCG GCCCCGCGGC GCCCGATGGC 
CCCGGCCTGG CCTCTGCCTG TAGCCTGAGC TCCCTGCGAG AGCCCGAGGC GCTGCCCCGC 
AAGGAGCACA CTCATCCGTC GGCCGCGGAC GGCCCGCAGG CGCCCCGTGA TCGCAGCAGC 
TCCTTCGCCA GCGGACGCCT AGTCGGGGAG CGACGTCGCT GGGACCCTCA GGTCCCCAGG 
CAGCTGCTCA GCGGAGCGAA CTGCGAACCG AGGGGCGTCC AGAGGATGGA CGGGGCCCCG 
GGCGGGCCGC CTTCCTGGGG CATGGTGGCC GGGAAGGCCG GGAAGTCCAA GTCAGCCGAG 
GATCTGCTGG AGCGCTCCGA CACACTGGCG GTCCCTGTGC ATGTGAGGTC CAGGTCGTCT 
CCCACCTCCG ACAAGAAAGG TCAGGATGTG CTCCTGAGGG AAGGCAGCAA CTTTGGTTTT 
GTAAAGGATC CATGCTGTTT GGCTGGCCCT GGACCTAGGT CTCTCAGCTG CTCAGACAAA 
GGCCAGAATG AGCTGGCATT GCCCCTGCAC CACCCTACGC CCTGCTGGAA CGGCTCAGGC 
TGCAAAGCCA CTGTGGCTTC CTCAGCTCCT CCCGAATCTT CTGGAGCCGC GGATCATCTG 
AAGCAACGTA GAGCGCCTGG TCCTAGGCCA CTCTCAGCAG GAATGCACGG CCACTTCCCA 
GACGCCAGAG CTGCCTCACT CAGCTCCCCT CTGCCATCAC CTGTCCCCTC CAGTTACCGG 
TCACAGCTTG CCATGGATCA GCAGACTGGG CAACAGCCTC CCTCATCCCC TGCCTCTGCA 
GTGACCCAGC CTACCAGTCC AAGAAGCCCT GAGCTCAGCA GCCCAGCATA TGGGCTAGGG 
GAGGGGATGT GGAAGAGGAC ATCCCTGCCT CAGCGACCCC CTCCCCCTTG GGTGAAATGG 
GCCCACGCGG TCAGAGAGGA CGGCCTTGCT GAGGACACCT TGGCGCCTGA GTTTGCGAAC 
CTGAAGCACT ATAGAAACCA GCCGAGTCGT CCGAGTTCCT GCAGCACTTC TGACCCAGAC 
ACTCCAGGAA GGATCTCGCT CCGAATATCG GAGTCAGCCC TTCAACCTTC CCCACCGCCC 
CGGGGGGACT ATGATGACGA AGTGTTCATG AAGGACTTGC ACCCCAAGGT TACTTCAAGC 
CCCACATTTG AAGCTCTTCC CCCACCCCCG CCACCTTCGC CACCGAGCGA GGAACCTCTA 
GTGAATGGCA CAGATGACTT TCCTCCGCCT CCTCCTCCCC AAGCCCTGTG TGAGGTACTG 
CTGGATGGTG AGGCATCCAC GGAAGCTGGC AGCGGCCCCT GCAGAATTCC CAGGGTGATG 
GTCACAAGGG AAGGGCACGT GCCTGGGGCA GCCCATTCCG AAGGTAGTCA GATAATGACT 
GCCACACCAC CCCAAACCTC GGCCAAAGGT TCAGAAGCCG AGTCCAACAC GCCTTCCAGC 
GCGAGCGCCC AGCCTCAGCT CAACGGTTCT CCTGGCAAGC AGCTCTGTCC CAGCCAGACC 
CGAAACCTCA CTTATGAACC TGTCGAGAGA ACTCAGGATC TAGGAAAGAA AACTCACGCC 
GAGCCTCAGA AGACCTCAGA AGATATCCGG ACAGAGGCTC TAGCCAAGGA AATTGTCCAC 
CAAGACAAGT CTCTGGCAGA CATTTTAGAT CCTGACTCCA GGATGAAGAC AACGATGGAC 
CTGATGGAGG GTTTATTCCC TGGAGACGCC AGTGTGCTGA TGGATAGTGG CGCAAAGAGG 
AAAGCCTTAG ACATCACCGC CAGGCGTGCG GGATGTGAAG CCAAGGCGAG CGACCACAAG 
GAGGCAGTGA GTGTGCTGGT CAACTGTCCT GCCTACTACA GTGTCTCTGC TGCCAAGGCG 
GAGCTGCTCA ACAAAATCAA AGACATGCCG GAGGAGCTGC AGGAGGAGGA GGGGCAGGAG 
GATGTCAATG AGAAAAAGGC TGAGCTTATC GGAAGCCTTA CCCACAAGCT GGAGAGCCTC 
CAGGAAGCCA AGGGGAGCCT GCTCACGGAC ATCAAGCTGA ACAATGCCCT GGGAGAGGAG 
GTGGAGGCTC TGATCAGTGA GCTCTGCAAA CCCAACGAGT TTGACAAGTA CAAGATGTTC 
ATAGGGGACC TGGACAAGGT GGTCAACCTG CTGCTGTCCC TGTCTGGACG CCTGGCCCGC 
GTGGAGAACG TCCTCAGAGG CCTTGGTGAA GACGCCAGCA AAGAGGAAAG GAGTTCTCTG 
AACGAGAAAA GGAAGGTTCT GGCTGGCCAG CATGAGGATG CACGGGAGCT CAAGGAGAAC 
CTGGACCGGA GGGAGCGCGT GGTGCTGGAT ATCCTGGCCA ACTACCTGTC CGCCGAGCAG 
CTGCAGGACT ACCAGCACTT CGTGAAGATG AAGTCCACAC TCCTCATTGA GCAGCGGAAG 
CTGGATGACA AGATAAAGCT GGGCCAGGAG CAGGTCAGGT GCCTGCTGGA GTCGCTTCCC 
TCGGACTTCA GGCCCAAGGC AGGGGCCATC TCCCTCCCTC CAGCCCTCAC CGGCCATGGG 
ACTCCTGGGG GGACATCTGT ATTCGGTGGT GTTTTCCCAA CGTTGACCTC TCCTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Lens placode planar cell polarity is dependent on Cdc42-mediated junctional contraction inhibition.
Developmental biology412(1)32-43(2016 Apr)
Muccioli M,Qaisi D,Herman K,Plageman TF Jr

Thyroid bud morphogenesis requires CDC42- and SHROOM3-dependent apical constriction.
Biology open5(2)130-9(2016 Jan)
Loebel DA,Plageman TF Jr,Tang TL,Jones VJ,Muccioli M,Tam PP

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Shroom3 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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