Abcg5 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Abcg5
Entrez Gene ID	27409
Full Name	ATP binding cassette subfamily G member 5
Synonyms	cmp,sterolin-1,trac
General protein information	Preferred Names ATP binding cassette subfamily G member 5 Names ATP-binding cassette sub-family G member 5 ATP-binding cassette, sub-family G (WHITE), member 5 sterolin 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17 E4\|17 55.02 cM
Summary	The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily, and functions as a half-transporter to limit intestinal absorption and promote biliary excretion of sterols. Disruption of this gene in mice results in thrombocytopenia, prolonged bleeding times, anemia, leukopenia, infertility, shortened life span and cardiomyopathy. Mice lacking this gene show symptoms of sitosterolemia. [provided by RefSeq, Nov 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_031884.2	NP_114090.1	ATP-binding cassette sub-family G member 5

Pathways from BioSystems

Homologies

Homo sapiens (human)	ABCG5	NP_071881.1
Canis lupus familiaris (dog)	ABCG5	XP_538475.1
Rattus norvegicus (Norway rat)	Abcg5	NP_446206.2
Danio rerio (zebrafish)	abcg5	NP_001122162.1
Bos taurus (cattle)	ABCG5	NP_001019718.1
Mus musculus (house mouse)	Abcg5	NP_114090.1
Drosophila melanogaster (fruit fly)	CG11069	NP_651307.2
Xenopus tropicalis (tropical clawed frog)	abcg5	NP_001120613.1
Pan troglodytes (chimpanzee)	ABCG5	XP_003309098.1
	ABCG5	XP_001111277.1
Gallus gallus (chicken)	ABCG5	XP_419457.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002051	XP_320996.5

Gene Ontology
Bibliography

Related articles in PubMed

Inhibition of Ceramide Decreased the Expression of ATP-Binding Cassette Transporter G5/8 mRNA in an Animal Model of Cholesterol Gallstone.
Kim HJ, Kim JS, Oh S, Yoo HS
Digestive diseases (Basel, Switzerland)35(5)439-443(2017)

Evidence that the adenosine triphosphate-binding cassette G5/G8-independent pathway plays a determinant role in cholesterol gallstone formation in mice.
Wang HH, Li X, Patel SB, Wang DQ
Hepatology (Baltimore, Md.)64(3)853-64(2016 09)

Hepatic ABCG5/G8 overexpression substantially increases biliary cholesterol secretion but does not impact in?vivo macrophage-to-feces RCT.
Dikkers A, de Boer JF, Groen AK, Tietge UJ
Atherosclerosis243(2)402-6(2015 Dec)

GRP78 rescues the ABCG5 ABCG8 sterol transporter in db/db mice.
Wang Y, Su K, Sabeva NS, Ji A, van der Westhuyzen DR, Foufelle F, Gao X, Graf GA
Metabolism: clinical and experimental64(11)1435-43(2015 Nov)

The mouse mutation "thrombocytopenia and cardiomyopathy" (trac) disrupts Abcg5: a spontaneous single gene model for human hereditary phytosterolemia/sitosterolemia.
Chase TH, Lyons BL, Bronson RT, Foreman O, Donahue LR, Burzenski LM, Gott B, Lane P, Harris B, Ceglarek U, Thiery J, Wittenburg H, Thon JN, Italiano JE Jr, Johnson KR, Shultz LD
Blood115(6)1267-76(2010 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ABCG5 and ABCG8 mRNA levels were significantly increased in cholesterol group and less increased in myriocin group, relative to that in normal group.
Title: Inhibition of Ceramide Decreased the Expression of ATP-Binding Cassette Transporter G5/8 mRNA in an Animal Model of Cholesterol Gallstone.

The ABCG5/G8-independent pathway plays an important role in regulating biliary cholesterol secretion, and gallstone formation, which works independently of the ABCG5/G8 pathway.
Title: Evidence that the adenosine triphosphate-binding cassette G5/G8-independent pathway plays a determinant role in cholesterol gallstone formation in mice.

ABCG5/G8 mediate mass biliary cholesterol secretion but not from a reverse cholesterol transport-relevant pool.
Title: Hepatic ABCG5/G8 overexpression substantially increases biliary cholesterol secretion but does not impact in?vivo macrophage-to-feces RCT.

AdGRP78 reduced expression of lipogenic genes and plasma triglycerides in the db/db strain. Both G5 and G8 protein levels increased as did total biliary cholesterol
Title: GRP78 rescues the ABCG5 ABCG8 sterol transporter in db/db mice.

The data demonstrate that Abcg5/Abcg8 deficiency reduces the uptake and secretion of both dietary triacylglycerols and cholesterol by the intestine, suggesting a novel role for the sterol transporter in the formation and secretion of chylomicrons.
Title: ABCG5/G8 deficiency in mice reduces dietary triacylglycerol and cholesterol transport into the lymph.

The following Abcg5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Abcg5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu83500	NM_031884.2 Latest version!	Mus musculus ATP binding cassette subfamily G member 5 (Abcg5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Abcg5 ( NM_031884.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_114090.1 Mus musculus ATP binding cassette subfamily G member 5 (Abcg5), mRNA.

Price & Availability↑

CloneID	OMu83500
Clone ID Related Accession (Same CDS sequence)	NM_031884.2
Accession Version	NM_031884.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1959bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Mus musculus(house mouse)
Product	ATP-binding cassette sub-family G member 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF312713.2 and BB798401.1. On Nov 25, 2015 this sequence version replaced NM_031884.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF312713.2, AK149569.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGGTGAGC TGCCCTTTCT GAGTCCAGAG GGAGCCAGAG GGCCTCACAT CAACAGAGGG 
TCTCTGAGCT CCCTGGAGCA AGGTTCGGTC ACGGGCACAG AGGCTCGGCA CAGCTTAGGT 
GTCCTGCATG TGTCCTACAG CGTCAGCAAC CGTGTCGGGC CTTGGTGGAA CATCAAATCA 
TGCCAGCAGA AGTGGGACAG GCAAATCCTC AAAGATGTCT CCTTGTACAT CGAGAGTGGC 
CAGATTATGT GCATCTTAGG CAGCTCAGGC TCAGGGAAGA CCACGCTGCT GGACGCCATC 
TCCGGGAGGC TGCGGCGCAC TGGGACCCTG GAAGGGGAGG TGTTTGTGAA TGGCTGCGAG 
CTGCGCAGGG ACCAGTTCCA AGACTGCTTC TCCTACGTCC TGCAGAGCGA CGTTTTTCTG 
AGCAGCCTCA CTGTGCGCGA GACGTTGCGA TACACAGCGA TGCTGGCCCT CTGCCGCAGC 
TCCGCGGACT TCTACAACAA GAAGGTAGAG GCAGTCATGA CAGAGCTGAG CCTGAGCCAC 
GTGGCGGACC AAATGATTGG CAGCTATAAT TTTGGGGGAA TTTCCAGTGG CGAGCGGCGC 
CGAGTTTCCA TCGCAGCCCA ACTCCTTCAG GACCCCAAGG TCATGATGCT AGATGAGCCA 
ACCACAGGAC TGGACTGCAT GACTGCAAAT CAAATTGTCC TTCTCTTGGC TGAGCTGGCT 
CGCAGGGACC GAATTGTGAT TGTCACCATC CACCAGCCTC GCTCTGAGCT CTTCCAACAC 
TTCGACAAAA TTGCCATCCT GACTTACGGA GAGTTGGTGT TCTGTGGCAC CCCAGAGGAG 
ATGCTTGGCT TCTTCAATAA CTGTGGTTAC CCCTGTCCTG AACATTCCAA TCCCTTTGAT 
TTTTACATGG ACTTGACATC AGTGGACACC CAAAGCAGAG AGCGGGAAAT AGAAACGTAC 
AAGCGAGTAC AGATGCTGGA ATGTGCCTTC AAGGAATCTG ACATCTATCA CAAAATTCTG 
GAGAACATTG AAAGAGCACG ATACCTGAAA ACCTTACCCA CGGTTCCTTT CAAAACAAAA 
GATCCTCCTG GGATGTTCGG CAAGCTTGGT GTCCTGCTGA GGCGAGTAAC AAGAAACTTA 
ATGAGGAATA AGCAGGCAGT GATTATGCGT CTCGTTCAGA ATCTGATCAT GGGCCTCTTC 
CTCATTTTCT ACCTTCTCCG CGTCCAGAAC AACACGCTAA AGGGCGCTGT GCAGGACCGC 
GTGGGGCTGC TCTATCAGCT TGTGGGTGCC ACCCCATACA CCGGCATGCT CAATGCTGTG 
AATCTGTTTC CCATGCTGAG AGCCGTCAGC GACCAGGAGA GTCAGGATGG CCTGTATCAT 
AAGTGGCAGA TGCTGCTCGC CTACGTGCTA CACGTCCTCC CCTTCAGCGT CATCGCCACG 
GTCATTTTCA GCAGTGTGTG TTATTGGACT CTGGGCTTGT ATCCTGAAGT TGCCAGATTT 
GGATATTTCT CTGCTGCTCT TTTGGCCCCT CACTTAATTG GAGAATTTCT AACACTTGTG 
CTGCTTGGTA TAGTCCAAAA CCCTAATATT GTCAACAGTA TAGTGGCTCT GCTCAGCATC 
TCTGGGCTGC TTATTGGATC TGGATTTATC AGAAACATAC AAGAAATGCC CATTCCTTTA 
AAAATCCTGG GTTATTTTAC ATTCCAAAAA TACTGTTGTG AGATTCTCGT GGTCAATGAG 
TTTTACGGCC TGAACTTCAC TTGTGGTGGA TCCAACACCT CTATGCTAAA TCACCCGATG 
TGCGCCATCA CCCAAGGGGT CCAGTTCATC GAGAAAACCT GCCCAGGTGC TACATCCAGA 
TTCACGGCAA ACTTCCTCAT CTTATATGGG TTTATCCCAG CTCTGGTCAT CCTAGGAATA 
GTGATTTTTA AAGTCAGGGA CTACCTGATT AGCAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_114090.1
CDS	139..2097
Translation	MGELPFLSPEGARGPHINRGSLSSLEQGSVTGTEARHSLGVLHVSYSVSNRVGPWWNIKSCQQKWDRQILKDVSLYIESGQIMCILGSSGSGKTTLLDAISGRLRRTGTLEGEVFVNGCELRRDQFQDCFSYVLQSDVFLSSLTVRETLRYTAMLALCRSSADFYNKKVEAVMTELSLSHVADQMIGSYNFGGISSGERRRVSIAAQLLQDPKVMMLDEPTTGLDCMTANQIVLLLAELARRDRIVIVTIHQPRSELFQHFDKIAILTYGELVFCGTPEEMLGFFNNCGYPCPEHSNPFDFYMDLTSVDTQSREREIETYKRVQMLECAFKESDIYHKILENIERARYLKTLPTVPFKTKDPPGMFGKLGVLLRRVTRNLMRNKQAVIMRLVQNLIMGLFLIFYLLRVQNNTLKGAVQDRVGLLYQLVGATPYTGMLNAVNLFPMLRAVSDQESQDGLYHKWQMLLAYVLHVLPFSVIATVIFSSVCYWTLGLYPEVARFGYFSAALLAPHLIGEFLTLVLLGIVQNPNIVNSIVALLSISGLLIGSGFIRNIQEMPIPLKILGYFTFQKYCCEILVVNEFYGLNFTCGGSNTSMLNHPMCAITQGVQFIEKTCPGATSRFTANFLILYGFIPALVILGIVIFKVRDYLISR

Target ORF information:

RefSeq Version	NM_031884.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ATP binding cassette subfamily G member 5 (Abcg5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031884.2

ORF Insert Sequence:

ATGGGTGAGC TGCCCTTTCT GAGTCCAGAG GGAGCCAGAG GGCCTCACAT CAACAGAGGG 
TCTCTGAGCT CCCTGGAGCA AGGTTCGGTC ACGGGCACAG AGGCTCGGCA CAGCTTAGGT 
GTCCTGCATG TGTCCTACAG CGTCAGCAAC CGTGTCGGGC CTTGGTGGAA CATCAAATCA 
TGCCAGCAGA AGTGGGACAG GCAAATCCTC AAAGATGTCT CCTTGTACAT CGAGAGTGGC 
CAGATTATGT GCATCTTAGG CAGCTCAGGC TCAGGGAAGA CCACGCTGCT GGACGCCATC 
TCCGGGAGGC TGCGGCGCAC TGGGACCCTG GAAGGGGAGG TGTTTGTGAA TGGCTGCGAG 
CTGCGCAGGG ACCAGTTCCA AGACTGCTTC TCCTACGTCC TGCAGAGCGA CGTTTTTCTG 
AGCAGCCTCA CTGTGCGCGA GACGTTGCGA TACACAGCGA TGCTGGCCCT CTGCCGCAGC 
TCCGCGGACT TCTACAACAA GAAGGTAGAG GCAGTCATGA CAGAGCTGAG CCTGAGCCAC 
GTGGCGGACC AAATGATTGG CAGCTATAAT TTTGGGGGAA TTTCCAGTGG CGAGCGGCGC 
CGAGTTTCCA TCGCAGCCCA ACTCCTTCAG GACCCCAAGG TCATGATGCT AGATGAGCCA 
ACCACAGGAC TGGACTGCAT GACTGCAAAT CAAATTGTCC TTCTCTTGGC TGAGCTGGCT 
CGCAGGGACC GAATTGTGAT TGTCACCATC CACCAGCCTC GCTCTGAGCT CTTCCAACAC 
TTCGACAAAA TTGCCATCCT GACTTACGGA GAGTTGGTGT TCTGTGGCAC CCCAGAGGAG 
ATGCTTGGCT TCTTCAATAA CTGTGGTTAC CCCTGTCCTG AACATTCCAA TCCCTTTGAT 
TTTTACATGG ACTTGACATC AGTGGACACC CAAAGCAGAG AGCGGGAAAT AGAAACGTAC 
AAGCGAGTAC AGATGCTGGA ATGTGCCTTC AAGGAATCTG ACATCTATCA CAAAATTCTG 
GAGAACATTG AAAGAGCACG ATACCTGAAA ACCTTACCCA CGGTTCCTTT CAAAACAAAA 
GATCCTCCTG GGATGTTCGG CAAGCTTGGT GTCCTGCTGA GGCGAGTAAC AAGAAACTTA 
ATGAGGAATA AGCAGGCAGT GATTATGCGT CTCGTTCAGA ATCTGATCAT GGGCCTCTTC 
CTCATTTTCT ACCTTCTCCG CGTCCAGAAC AACACGCTAA AGGGCGCTGT GCAGGACCGC 
GTGGGGCTGC TCTATCAGCT TGTGGGTGCC ACCCCATACA CCGGCATGCT CAATGCTGTG 
AATCTGTTTC CCATGCTGAG AGCCGTCAGC GACCAGGAGA GTCAGGATGG CCTGTATCAT 
AAGTGGCAGA TGCTGCTCGC CTACGTGCTA CACGTCCTCC CCTTCAGCGT CATCGCCACG 
GTCATTTTCA GCAGTGTGTG TTATTGGACT CTGGGCTTGT ATCCTGAAGT TGCCAGATTT 
GGATATTTCT CTGCTGCTCT TTTGGCCCCT CACTTAATTG GAGAATTTCT AACACTTGTG 
CTGCTTGGTA TAGTCCAAAA CCCTAATATT GTCAACAGTA TAGTGGCTCT GCTCAGCATC 
TCTGGGCTGC TTATTGGATC TGGATTTATC AGAAACATAC AAGAAATGCC CATTCCTTTA 
AAAATCCTGG GTTATTTTAC ATTCCAAAAA TACTGTTGTG AGATTCTCGT GGTCAATGAG 
TTTTACGGCC TGAACTTCAC TTGTGGTGGA TCCAACACCT CTATGCTAAA TCACCCGATG 
TGCGCCATCA CCCAAGGGGT CCAGTTCATC GAGAAAACCT GCCCAGGTGC TACATCCAGA 
TTCACGGCAA ACTTCCTCAT CTTATATGGG TTTATCCCAG CTCTGGTCAT CCTAGGAATA 
GTGATTTTTA AAGTCAGGGA CTACCTGATT AGCAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Inhibition of Ceramide Decreased the Expression of ATP-Binding Cassette Transporter G5/8 mRNA in an Animal Model of Cholesterol Gallstone.
Digestive diseases (Basel, Switzerland)35(5)439-443(2017)
Kim HJ,Kim JS,Oh S,Yoo HS

Evidence that the adenosine triphosphate-binding cassette G5/G8-independent pathway plays a determinant role in cholesterol gallstone formation in mice.
Hepatology (Baltimore, Md.)64(3)853-64(2016 09)
Wang HH,Li X,Patel SB,Wang DQ

Hepatic ABCG5/G8 overexpression substantially increases biliary cholesterol secretion but does not impact in?vivo macrophage-to-feces RCT.
Atherosclerosis243(2)402-6(2015 Dec)
Dikkers A,de Boer JF,Groen AK,Tietge UJ

GRP78 rescues the ABCG5 ABCG8 sterol transporter in db/db mice.
Metabolism: clinical and experimental64(11)1435-43(2015 Nov)
Wang Y,Su K,Sabeva NS,Ji A,van der Westhuyzen DR,Foufelle F,Gao X,Graf GA

The mouse mutation "thrombocytopenia and cardiomyopathy" (trac) disrupts Abcg5: a spontaneous single gene model for human hereditary phytosterolemia/sitosterolemia.
Blood115(6)1267-76(2010 Feb)
Chase TH,Lyons BL,Bronson RT,Foreman O,Donahue LR,Burzenski LM,Gott B,Lane P,Harris B,Ceglarek U,Thiery J,Wittenburg H,Thon JN,Italiano JE Jr,Johnson KR,Shultz LD

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Abcg5 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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