Sytl4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sytl4
Entrez Gene ID	27359
Full Name	synaptotagmin-like 4
Synonyms	Gph,Slp4
General protein information	Preferred Names synaptotagmin-like 4 Names synaptotagmin-like protein 4 exophilin-2 granuphilin-a granuphilin-b
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: X Map Location: X\|X E3

mRNA and Protein(s)

mRNA	Protein	Name
NM_013757.2	NP_038785.1	synaptotagmin-like protein 4 isoform 1
NM_001290718.1	NP_001277647.1	synaptotagmin-like protein 4 isoform 2
XM_006528551.2	XP_006528614.1	synaptotagmin-like protein 4 isoform X1
NM_001290717.1	NP_001277646.1	synaptotagmin-like protein 4 isoform 2
NM_001290719.1	NP_001277648.1	synaptotagmin-like protein 4 isoform 3
XM_006528551.3	XP_006528614.1	synaptotagmin-like protein 4 isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Sytl4	NP_536335.1
Canis lupus familiaris (dog)	SYTL4	XP_005641613.1
Mus musculus (house mouse)	Sytl4	NP_038785.1
Danio rerio (zebrafish)	LOC100535893	XP_003199981.2
Homo sapiens (human)	SYTL4	NP_001123368.1
Danio rerio (zebrafish)	sytl4	NP_001074457.1
Xenopus tropicalis (tropical clawed frog)	sytl4	XP_002940484.1
Pan troglodytes (chimpanzee)	SYTL4	XP_003317604.1
	LOC700889	XP_002808587.1
Bos taurus (cattle)	SYTL4	NP_001179362.1
Drosophila melanogaster (fruit fly)	btsz	NP_001262582.1
Gallus gallus (chicken)	SYTL4	XP_004940758.1

Gene Ontology
Bibliography

Related articles in PubMed

?-Cell-Specific Mafk Overexpression Impairs Pancreatic Endocrine Cell Development.
Abdellatif AM, Oishi H, Itagaki T, Jung Y, Shawki HH, Okita Y, Hasegawa Y, Suzuki H, El-Morsy SE, El-Sayed MA, Shoaib MB, Sugiyama F, Takahashi S
PloS one11(2)e0150010(2016)

The Rab27a effector exophilin7 promotes fusion of secretory granules that have not been docked to the plasma membrane.
Wang H, Ishizaki R, Xu J, Kasai K, Kobayashi E, Gomi H, Izumi T
Molecular biology of the cell24(3)319-30(2013 Feb)

Modular genetic control of sexually dimorphic behaviors.
Xu X, Coats JK, Yang CF, Wang A, Ahmed OM, Alvarado M, Izumi T, Shah NM
Cell148(3)596-607(2012 Feb)

Loss of granuphilin and loss of syntaxin-1A cause differential effects on insulin granule docking and fusion.
Wang H, Ishizaki R, Kobayashi E, Fujiwara T, Akagawa K, Izumi T
The Journal of biological chemistry286(37)32244-50(2011 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Granuphilin and exophilin7, differentially regulate the exocytosis of either stably or minimally docked granules, respectively.
Title: The Rab27a effector exophilin7 promotes fusion of secretory granules that have not been docked to the plasma membrane.

granuphilin binds to syntaxin-2 and syntaxin-3 as well as syntaxin-1a, it likely mediates granule docking through interactions with those multiple syntaxins on the plasma membrane.
Title: Loss of granuphilin and loss of syntaxin-1A cause differential effects on insulin granule docking and fusion.

It is shown that the granuphilin promoter is a target of SREBP-1c, a transcription factor that controls fatty acid synthesis, and MafA, a beta cell differentiation factor
Title: Granuphilin is activated by SREBP-1c and involved in impaired insulin secretion in diabetic mice.

granuphilin promotes the plasma membrane targeting of insulin granules via interaction with syntaxin 1a
Title: Rab27 effector granuphilin promotes the plasma membrane targeting of insulin granules via interaction with syntaxin 1a.

Synaptotagmin-like protein 4 (Slp4)/granuphilin-a contains an N-terminal Slp homology domain (SHD) (PMID: 11327731). The SHD of Slp4 specifically and directly binds the GTP-bound form of Rab3A, Rab8, and Rab27A.
Title: The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain.

The following Sytl4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sytl4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu17017	NM_001290718.1 Latest version!	Mus musculus synaptotagmin-like 4 (Sytl4), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu17017	NM_001290717.1 Latest version!	Mus musculus synaptotagmin-like 4 (Sytl4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu14296	NM_013757.2 Latest version!	Mus musculus synaptotagmin-like 4 (Sytl4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu14322	NM_001290719.1 Latest version!	Mus musculus synaptotagmin-like 4 (Sytl4), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OMu14296	XM_006528551.3 Latest version!	Mus musculus synaptotagmin-like 4 (Sytl4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OMu14296	XM_006528551.2	Mus musculus synaptotagmin-like 4 (Sytl4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sytl4 ( NM_001290718.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001277647.1 Mus musculus synaptotagmin-like 4 (Sytl4), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu17017
Clone ID Related Accession (Same CDS sequence)	NM_001290718.1 , NM_001290717.1
Accession Version	NM_001290718.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1509bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	synaptotagmin-like protein 4 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX510358.4, AK030401.1 and AB025258.1. On Mar 19, 2014 this sequence version replaced XM_006528553.1. Transcript Variant: This variant (3) uses an alternate splice site in the 5' UTR and lacks three alternate exons, which results in a frameshift, compared to variant 1. The encoded isoform (2) has a distinct C-terminus and is shorter than isoform 1. Variants 2 and 3 encode the same isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK030401.1, SRR1660825.77123.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGGAGA TACTAGACCT CTCTTTTCTG TCCGAGATGG AAAGGGATTT GATCCTCGGT 
GTTCTGCAGA GAGATGAAGA ACTCAGAAAA GCAGATGAGA AAAGGATTCG GCGACTGAAG 
AATGAGCTCC TGGAGATCAA AAGAAAAGGG GCCAAAAGGG GCAGCCAACA CTATAGTGAT 
CGAACCTGTG CCAGGTGCCA GGAGGGCCTG GGCCGCTTAA TTCCCAAAAG CAGCACTTGT 
GTGGGCTGCA ATCATTTAGT GTGCCGAGAA TGCCGAGTTC TGGAAAGCAA TGGCTCTTGG 
AGGTGCAAGG TGTGCTCGAA GGAGATAGAG CTGAAGAAAG CAACTGGAGA TTGGTTTTAT 
GACCAGAAAG TAAATCGCTT TGATTACCGA ACAGGCAGCG AGATAATCAG GATGTCCCTG 
CGCCAGAAGC CTGCAGTGAA TAAAAGAGAG ACAGCGGGGC AGTCCCTCCT TCAGCAGACA 
CAGATGGGTG ATATCTGGCC AGGAAGAAGG ATCATTCAAG AACAACAACA GAGGGAACAA 
AGTGTGCTGT TTGAAGTGCC AAAGACCAGA AGTGGAAAGA GTGCACTGGA GGCTGAGAGC 
GAGAGTCTGG ATAGCTACAC TGCTGACTCA GACAGCACCT CCAGGAGAGA TTCTCTGGAT 
AAATCTGGAC TCTTCCCAGA ATGGAAGAAG ATGTCCGCTC CCAAATCTCA AGTTGAAAAG 
GAAATTCCAC CAGGGAACCA AAATGCAGTC TGTGGTGATG AGGGTGACAT GGTATTCAAG 
AAGAACACCA AAAAAGTCCT GAGGCCTTCA GAATACACTA AGTCTGTGAT AGACCTTCGC 
CCAGAAGATG TGGCCCAAGA AAGTGGCATC TTGGGAGACA GAAGCAAATC TGTACCAGGC 
CTCAGTGTGG ATATGGAAGA AGAGGAGGAG GAGGAAGAGG ACATTGACCA CCTGGTGAAG 
TTGCACCGCC AGAAACTGGC CAGAGGCAGC ATGCAGAGTG GCTCCTCCAT GAGTACCCTT 
GGCAGCATTA TGAGTATCTA TAGCGAAGCT GGTGATTTTG GGAACATCTC TGTGACGGGC 
AAGATTGCCT TTTCACTGAA GTTTGAGCAG AAAACACAGA CTTTGGTCAT CCATGTCAAG 
GAGTGCCACC AGCTGGCCTA TGCTGATGAA GCCAAGAAGC GTTCTAACCC ATATGTGAAG 
ACTTATCTTC TGCCTGACAA GTCCCGCCAA GGAAAAAGAA AAACCAGCAT CAAGCGGGAC 
ACCATCAATC CACTATATGA TGAGACCTTT CGGTACGAGA TTTCGGAATC TCTTCTGGCT 
CAGAGGACTT TGCAGTTTTC CGTTTGGCAT CATGGTCGTT TTGGCAGAAA CACTTTCCTT 
GGAGAGGCGG AGGTCCACAT GGACTCTTGG AAGCTGGATA AGAAGCTGGA TCACTGCCTC 
CCTTTACATG GAAAGGGATC AGTAATGGCG AAGTGGTGGA CTGGATGGAT TCGACTGGTG 
AAGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001277647.1
CDS	188..1696
Translation	MSEILDLSFLSEMERDLILGVLQRDEELRKADEKRIRRLKNELLEIKRKGAKRGSQHYSDRTCARCQEGLGRLIPKSSTCVGCNHLVCRECRVLESNGSWRCKVCSKEIELKKATGDWFYDQKVNRFDYRTGSEIIRMSLRQKPAVNKRETAGQSLLQQTQMGDIWPGRRIIQEQQQREQSVLFEVPKTRSGKSALEAESESLDSYTADSDSTSRRDSLDKSGLFPEWKKMSAPKSQVEKEIPPGNQNAVCGDEGDMVFKKNTKKVLRPSEYTKSVIDLRPEDVAQESGILGDRSKSVPGLSVDMEEEEEEEEDIDHLVKLHRQKLARGSMQSGSSMSTLGSIMSIYSEAGDFGNISVTGKIAFSLKFEQKTQTLVIHVKECHQLAYADEAKKRSNPYVKTYLLPDKSRQGKRKTSIKRDTINPLYDETFRYEISESLLAQRTLQFSVWHHGRFGRNTFLGEAEVHMDSWKLDKKLDHCLPLHGKGSVMAKWWTGWIRLVKK

Target ORF information:

RefSeq Version	NM_001290718.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus synaptotagmin-like 4 (Sytl4), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001290718.1

ORF Insert Sequence:

ATGTCGGAGA TACTAGACCT CTCTTTTCTG TCCGAGATGG AAAGGGATTT GATCCTCGGT 
GTTCTGCAGA GAGATGAAGA ACTCAGAAAA GCAGATGAGA AAAGGATTCG GCGACTGAAG 
AATGAGCTCC TGGAGATCAA AAGAAAAGGG GCCAAAAGGG GCAGCCAACA CTATAGTGAT 
CGAACCTGTG CCAGGTGCCA GGAGGGCCTG GGCCGCTTAA TTCCCAAAAG CAGCACTTGT 
GTGGGCTGCA ATCATTTAGT GTGCCGAGAA TGCCGAGTTC TGGAAAGCAA TGGCTCTTGG 
AGGTGCAAGG TGTGCTCGAA GGAGATAGAG CTGAAGAAAG CAACTGGAGA TTGGTTTTAT 
GACCAGAAAG TAAATCGCTT TGATTACCGA ACAGGCAGCG AGATAATCAG GATGTCCCTG 
CGCCAGAAGC CTGCAGTGAA TAAAAGAGAG ACAGCGGGGC AGTCCCTCCT TCAGCAGACA 
CAGATGGGTG ATATCTGGCC AGGAAGAAGG ATCATTCAAG AACAACAACA GAGGGAACAA 
AGTGTGCTGT TTGAAGTGCC AAAGACCAGA AGTGGAAAGA GTGCACTGGA GGCTGAGAGC 
GAGAGTCTGG ATAGCTACAC TGCTGACTCA GACAGCACCT CCAGGAGAGA TTCTCTGGAT 
AAATCTGGAC TCTTCCCAGA ATGGAAGAAG ATGTCCGCTC CCAAATCTCA AGTTGAAAAG 
GAAATTCCAC CAGGGAACCA AAATGCAGTC TGTGGTGATG AGGGTGACAT GGTATTCAAG 
AAGAACACCA AAAAAGTCCT GAGGCCTTCA GAATACACTA AGTCTGTGAT AGACCTTCGC 
CCAGAAGATG TGGCCCAAGA AAGTGGCATC TTGGGAGACA GAAGCAAATC TGTACCAGGC 
CTCAGTGTGG ATATGGAAGA AGAGGAGGAG GAGGAAGAGG ACATTGACCA CCTGGTGAAG 
TTGCACCGCC AGAAACTGGC CAGAGGCAGC ATGCAGAGTG GCTCCTCCAT GAGTACCCTT 
GGCAGCATTA TGAGTATCTA TAGCGAAGCT GGTGATTTTG GGAACATCTC TGTGACGGGC 
AAGATTGCCT TTTCACTGAA GTTTGAGCAG AAAACACAGA CTTTGGTCAT CCATGTCAAG 
GAGTGCCACC AGCTGGCCTA TGCTGATGAA GCCAAGAAGC GTTCTAACCC ATATGTGAAG 
ACTTATCTTC TGCCTGACAA GTCCCGCCAA GGAAAAAGAA AAACCAGCAT CAAGCGGGAC 
ACCATCAATC CACTATATGA TGAGACCTTT CGGTACGAGA TTTCGGAATC TCTTCTGGCT 
CAGAGGACTT TGCAGTTTTC CGTTTGGCAT CATGGTCGTT TTGGCAGAAA CACTTTCCTT 
GGAGAGGCGG AGGTCCACAT GGACTCTTGG AAGCTGGATA AGAAGCTGGA TCACTGCCTC 
CCTTTACATG GAAAGGGATC AGTAATGGCG AAGTGGTGGA CTGGATGGAT TCGACTGGTG 
AAGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sytl4 ( NM_001290717.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001277646.1 Mus musculus synaptotagmin-like 4 (Sytl4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu17017
Clone ID Related Accession (Same CDS sequence)	NM_001290718.1 , NM_001290717.1
Accession Version	NM_001290717.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1509bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Mus musculus(house mouse)
Product	synaptotagmin-like protein 4 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX510358.4, AK161582.1, AL691421.9 and AB025258.1. On Mar 19, 2014 this sequence version replaced XM_006528552.1. Transcript Variant: This variant (2) lacks three alternate exons, which results in a frameshift, compared to variant 1. The encoded isoform (2) has a distinct C-terminus and is shorter than isoform 1. Variants 2 and 3 encode the same isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB025259.1, AK161582.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGGAGA TACTAGACCT CTCTTTTCTG TCCGAGATGG AAAGGGATTT GATCCTCGGT 
GTTCTGCAGA GAGATGAAGA ACTCAGAAAA GCAGATGAGA AAAGGATTCG GCGACTGAAG 
AATGAGCTCC TGGAGATCAA AAGAAAAGGG GCCAAAAGGG GCAGCCAACA CTATAGTGAT 
CGAACCTGTG CCAGGTGCCA GGAGGGCCTG GGCCGCTTAA TTCCCAAAAG CAGCACTTGT 
GTGGGCTGCA ATCATTTAGT GTGCCGAGAA TGCCGAGTTC TGGAAAGCAA TGGCTCTTGG 
AGGTGCAAGG TGTGCTCGAA GGAGATAGAG CTGAAGAAAG CAACTGGAGA TTGGTTTTAT 
GACCAGAAAG TAAATCGCTT TGATTACCGA ACAGGCAGCG AGATAATCAG GATGTCCCTG 
CGCCAGAAGC CTGCAGTGAA TAAAAGAGAG ACAGCGGGGC AGTCCCTCCT TCAGCAGACA 
CAGATGGGTG ATATCTGGCC AGGAAGAAGG ATCATTCAAG AACAACAACA GAGGGAACAA 
AGTGTGCTGT TTGAAGTGCC AAAGACCAGA AGTGGAAAGA GTGCACTGGA GGCTGAGAGC 
GAGAGTCTGG ATAGCTACAC TGCTGACTCA GACAGCACCT CCAGGAGAGA TTCTCTGGAT 
AAATCTGGAC TCTTCCCAGA ATGGAAGAAG ATGTCCGCTC CCAAATCTCA AGTTGAAAAG 
GAAATTCCAC CAGGGAACCA AAATGCAGTC TGTGGTGATG AGGGTGACAT GGTATTCAAG 
AAGAACACCA AAAAAGTCCT GAGGCCTTCA GAATACACTA AGTCTGTGAT AGACCTTCGC 
CCAGAAGATG TGGCCCAAGA AAGTGGCATC TTGGGAGACA GAAGCAAATC TGTACCAGGC 
CTCAGTGTGG ATATGGAAGA AGAGGAGGAG GAGGAAGAGG ACATTGACCA CCTGGTGAAG 
TTGCACCGCC AGAAACTGGC CAGAGGCAGC ATGCAGAGTG GCTCCTCCAT GAGTACCCTT 
GGCAGCATTA TGAGTATCTA TAGCGAAGCT GGTGATTTTG GGAACATCTC TGTGACGGGC 
AAGATTGCCT TTTCACTGAA GTTTGAGCAG AAAACACAGA CTTTGGTCAT CCATGTCAAG 
GAGTGCCACC AGCTGGCCTA TGCTGATGAA GCCAAGAAGC GTTCTAACCC ATATGTGAAG 
ACTTATCTTC TGCCTGACAA GTCCCGCCAA GGAAAAAGAA AAACCAGCAT CAAGCGGGAC 
ACCATCAATC CACTATATGA TGAGACCTTT CGGTACGAGA TTTCGGAATC TCTTCTGGCT 
CAGAGGACTT TGCAGTTTTC CGTTTGGCAT CATGGTCGTT TTGGCAGAAA CACTTTCCTT 
GGAGAGGCGG AGGTCCACAT GGACTCTTGG AAGCTGGATA AGAAGCTGGA TCACTGCCTC 
CCTTTACATG GAAAGGGATC AGTAATGGCG AAGTGGTGGA CTGGATGGAT TCGACTGGTG 
AAGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001277646.1
CDS	215..1723
Translation	MSEILDLSFLSEMERDLILGVLQRDEELRKADEKRIRRLKNELLEIKRKGAKRGSQHYSDRTCARCQEGLGRLIPKSSTCVGCNHLVCRECRVLESNGSWRCKVCSKEIELKKATGDWFYDQKVNRFDYRTGSEIIRMSLRQKPAVNKRETAGQSLLQQTQMGDIWPGRRIIQEQQQREQSVLFEVPKTRSGKSALEAESESLDSYTADSDSTSRRDSLDKSGLFPEWKKMSAPKSQVEKEIPPGNQNAVCGDEGDMVFKKNTKKVLRPSEYTKSVIDLRPEDVAQESGILGDRSKSVPGLSVDMEEEEEEEEDIDHLVKLHRQKLARGSMQSGSSMSTLGSIMSIYSEAGDFGNISVTGKIAFSLKFEQKTQTLVIHVKECHQLAYADEAKKRSNPYVKTYLLPDKSRQGKRKTSIKRDTINPLYDETFRYEISESLLAQRTLQFSVWHHGRFGRNTFLGEAEVHMDSWKLDKKLDHCLPLHGKGSVMAKWWTGWIRLVKK

Target ORF information:

RefSeq Version	NM_001290717.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus synaptotagmin-like 4 (Sytl4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001290717.1

ORF Insert Sequence:

ATGTCGGAGA TACTAGACCT CTCTTTTCTG TCCGAGATGG AAAGGGATTT GATCCTCGGT 
GTTCTGCAGA GAGATGAAGA ACTCAGAAAA GCAGATGAGA AAAGGATTCG GCGACTGAAG 
AATGAGCTCC TGGAGATCAA AAGAAAAGGG GCCAAAAGGG GCAGCCAACA CTATAGTGAT 
CGAACCTGTG CCAGGTGCCA GGAGGGCCTG GGCCGCTTAA TTCCCAAAAG CAGCACTTGT 
GTGGGCTGCA ATCATTTAGT GTGCCGAGAA TGCCGAGTTC TGGAAAGCAA TGGCTCTTGG 
AGGTGCAAGG TGTGCTCGAA GGAGATAGAG CTGAAGAAAG CAACTGGAGA TTGGTTTTAT 
GACCAGAAAG TAAATCGCTT TGATTACCGA ACAGGCAGCG AGATAATCAG GATGTCCCTG 
CGCCAGAAGC CTGCAGTGAA TAAAAGAGAG ACAGCGGGGC AGTCCCTCCT TCAGCAGACA 
CAGATGGGTG ATATCTGGCC AGGAAGAAGG ATCATTCAAG AACAACAACA GAGGGAACAA 
AGTGTGCTGT TTGAAGTGCC AAAGACCAGA AGTGGAAAGA GTGCACTGGA GGCTGAGAGC 
GAGAGTCTGG ATAGCTACAC TGCTGACTCA GACAGCACCT CCAGGAGAGA TTCTCTGGAT 
AAATCTGGAC TCTTCCCAGA ATGGAAGAAG ATGTCCGCTC CCAAATCTCA AGTTGAAAAG 
GAAATTCCAC CAGGGAACCA AAATGCAGTC TGTGGTGATG AGGGTGACAT GGTATTCAAG 
AAGAACACCA AAAAAGTCCT GAGGCCTTCA GAATACACTA AGTCTGTGAT AGACCTTCGC 
CCAGAAGATG TGGCCCAAGA AAGTGGCATC TTGGGAGACA GAAGCAAATC TGTACCAGGC 
CTCAGTGTGG ATATGGAAGA AGAGGAGGAG GAGGAAGAGG ACATTGACCA CCTGGTGAAG 
TTGCACCGCC AGAAACTGGC CAGAGGCAGC ATGCAGAGTG GCTCCTCCAT GAGTACCCTT 
GGCAGCATTA TGAGTATCTA TAGCGAAGCT GGTGATTTTG GGAACATCTC TGTGACGGGC 
AAGATTGCCT TTTCACTGAA GTTTGAGCAG AAAACACAGA CTTTGGTCAT CCATGTCAAG 
GAGTGCCACC AGCTGGCCTA TGCTGATGAA GCCAAGAAGC GTTCTAACCC ATATGTGAAG 
ACTTATCTTC TGCCTGACAA GTCCCGCCAA GGAAAAAGAA AAACCAGCAT CAAGCGGGAC 
ACCATCAATC CACTATATGA TGAGACCTTT CGGTACGAGA TTTCGGAATC TCTTCTGGCT 
CAGAGGACTT TGCAGTTTTC CGTTTGGCAT CATGGTCGTT TTGGCAGAAA CACTTTCCTT 
GGAGAGGCGG AGGTCCACAT GGACTCTTGG AAGCTGGATA AGAAGCTGGA TCACTGCCTC 
CCTTTACATG GAAAGGGATC AGTAATGGCG AAGTGGTGGA CTGGATGGAT TCGACTGGTG 
AAGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sytl4 ( NM_013757.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_038785.1 Mus musculus synaptotagmin-like 4 (Sytl4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu14296
Clone ID Related Accession (Same CDS sequence)	NM_013757.2 , XM_006528551.2 , XM_006528551.3
Accession Version	NM_013757.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2022bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Mus musculus(house mouse)
Product	synaptotagmin-like protein 4 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX510358.4 and AB025258.1. On Mar 19, 2014 this sequence version replaced NM_013757.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB025258.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGGAGA TACTAGACCT CTCTTTTCTG TCCGAGATGG AAAGGGATTT GATCCTCGGT 
GTTCTGCAGA GAGATGAAGA ACTCAGAAAA GCAGATGAGA AAAGGATTCG GCGACTGAAG 
AATGAGCTCC TGGAGATCAA AAGAAAAGGG GCCAAAAGGG GCAGCCAACA CTATAGTGAT 
CGAACCTGTG CCAGGTGCCA GGAGGGCCTG GGCCGCTTAA TTCCCAAAAG CAGCACTTGT 
GTGGGCTGCA ATCATTTAGT GTGCCGAGAA TGCCGAGTTC TGGAAAGCAA TGGCTCTTGG 
AGGTGCAAGG TGTGCTCGAA GGAGATAGAG CTGAAGAAAG CAACTGGAGA TTGGTTTTAT 
GACCAGAAAG TAAATCGCTT TGATTACCGA ACAGGCAGCG AGATAATCAG GATGTCCCTG 
CGCCAGAAGC CTGCAGTGAA TAAAAGAGAG ACAGCGGGGC AGTCCCTCCT TCAGCAGACA 
CAGATGGGTG ATATCTGGCC AGGAAGAAGG ATCATTCAAG AACAACAACA GAGGGAACAA 
AGTGTGCTGT TTGAAGTGCC AAAGACCAGA AGTGGAAAGA GTGCACTGGA GGCTGAGAGC 
GAGAGTCTGG ATAGCTACAC TGCTGACTCA GACAGCACCT CCAGGAGAGA TTCTCTGGAT 
AAATCTGGAC TCTTCCCAGA ATGGAAGAAG ATGTCCGCTC CCAAATCTCA AGTTGAAAAG 
GAAATTCCAC CAGGGAACCA AAATGCAGTC TGTGGTGATG AGGGTGACAT GGTATTCAAG 
AAGAACACCA AAAAAGTCCT GAGGCCTTCA GAATACACTA AGTCTGTGAT AGACCTTCGC 
CCAGAAGATG TGGCCCAAGA AAGTGGCATC TTGGGAGACA GAAGCAAATC TGTACCAGGC 
CTCAGTGTGG ATATGGAAGA AGAGGAGGAG GAGGAAGAGG ACATTGACCA CCTGGTGAAG 
TTGCACCGCC AGAAACTGGC CAGAGGCAGC ATGCAGAGTG GCTCCTCCAT GAGTACCCTT 
GGCAGCATTA TGAGTATCTA TAGCGAAGCT GGTGATTTTG GGAACATCTC TGTGACGGGC 
AAGATTGCCT TTTCACTGAA GTTTGAGCAG AAAACACAGA CTTTGGTCAT CCATGTCAAG 
GAGTGCCACC AGCTGGCCTA TGCTGATGAA GCCAAGAAGC GTTCTAACCC ATATGTGAAG 
ACTTATCTTC TGCCTGACAA GTCCCGCCAA GGAAAAAGAA AAACCAGCAT CAAGCGGGAC 
ACCATCAATC CACTATATGA TGAGACCTTT CGGTACGAGA TTTCGGAATC TCTTCTGGCT 
CAGAGGACTT TGCAGTTTTC CGTTTGGCAT CATGGTCGTT TTGGCAGAAA CACTTTCCTT 
GGAGAGGCGG AGGTCCACAT GGACTCTTGG AAGCTGGATA AGAAGCTGGA TCACTGCCTC 
CCTTTACATG GAAAGATCAG TACTGAGTCC TCGCCTGGTT TGCCAGCACA CAAAGGCGAG 
TTGGTGGTTT CATTGAAATA CATCCCAGCC TCCAAACTCC CTGTTGGAGG TGACCGGAAA 
AAGAGTAAAG GTGGGGAAGG GGGAGAACTC CAGGTGTGGA TCAAAGAAGC CAAGAACCTG 
ACGGCTGCCA AATCAGGAGG GACTTCAGAC AGCTTTGTCA AGGGATACCT CCTTCCCATG 
AGGAACAAGG CCAGTAAGCG TAAAACTCCT GTGATGAAGA AGACTCTGAG TCCCCACTAC 
AACCATACAT TTGTCTACAA TGGTGTGAGG CTGGAAGATC TACAACACAT GTGCCTGGAA 
CTGACTGTAT GGGACCGGGA GCCCCTGGCC AGCAATGACT TCCTGGGAGG GGTCAGACTG 
GGTGTTGGCA CTGGGATCAG TAATGGCGAA GTGGTGGACT GGATGGATTC GACTGGTGAA 
GAAGTAAGCT TGTGGCAGAA GATGCGACAG TACCCAGGGT CTTGGGCAGA AGGAACTCTG 
CAGCTCCGTT CTTCAATGGT CAAGCAGAAG CTGGGTGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038785.1
CDS	215..2236
Translation	MSEILDLSFLSEMERDLILGVLQRDEELRKADEKRIRRLKNELLEIKRKGAKRGSQHYSDRTCARCQEGLGRLIPKSSTCVGCNHLVCRECRVLESNGSWRCKVCSKEIELKKATGDWFYDQKVNRFDYRTGSEIIRMSLRQKPAVNKRETAGQSLLQQTQMGDIWPGRRIIQEQQQREQSVLFEVPKTRSGKSALEAESESLDSYTADSDSTSRRDSLDKSGLFPEWKKMSAPKSQVEKEIPPGNQNAVCGDEGDMVFKKNTKKVLRPSEYTKSVIDLRPEDVAQESGILGDRSKSVPGLSVDMEEEEEEEEDIDHLVKLHRQKLARGSMQSGSSMSTLGSIMSIYSEAGDFGNISVTGKIAFSLKFEQKTQTLVIHVKECHQLAYADEAKKRSNPYVKTYLLPDKSRQGKRKTSIKRDTINPLYDETFRYEISESLLAQRTLQFSVWHHGRFGRNTFLGEAEVHMDSWKLDKKLDHCLPLHGKISTESSPGLPAHKGELVVSLKYIPASKLPVGGDRKKSKGGEGGELQVWIKEAKNLTAAKSGGTSDSFVKGYLLPMRNKASKRKTPVMKKTLSPHYNHTFVYNGVRLEDLQHMCLELTVWDREPLASNDFLGGVRLGVGTGISNGEVVDWMDSTGEEVSLWQKMRQYPGSWAEGTLQLRSSMVKQKLGV

Target ORF information:

RefSeq Version	NM_013757.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus synaptotagmin-like 4 (Sytl4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013757.2

ORF Insert Sequence:

ATGTCGGAGA TACTAGACCT CTCTTTTCTG TCCGAGATGG AAAGGGATTT GATCCTCGGT 
GTTCTGCAGA GAGATGAAGA ACTCAGAAAA GCAGATGAGA AAAGGATTCG GCGACTGAAG 
AATGAGCTCC TGGAGATCAA AAGAAAAGGG GCCAAAAGGG GCAGCCAACA CTATAGTGAT 
CGAACCTGTG CCAGGTGCCA GGAGGGCCTG GGCCGCTTAA TTCCCAAAAG CAGCACTTGT 
GTGGGCTGCA ATCATTTAGT GTGCCGAGAA TGCCGAGTTC TGGAAAGCAA TGGCTCTTGG 
AGGTGCAAGG TGTGCTCGAA GGAGATAGAG CTGAAGAAAG CAACTGGAGA TTGGTTTTAT 
GACCAGAAAG TAAATCGCTT TGATTACCGA ACAGGCAGCG AGATAATCAG GATGTCCCTG 
CGCCAGAAGC CTGCAGTGAA TAAAAGAGAG ACAGCGGGGC AGTCCCTCCT TCAGCAGACA 
CAGATGGGTG ATATCTGGCC AGGAAGAAGG ATCATTCAAG AACAACAACA GAGGGAACAA 
AGTGTGCTGT TTGAAGTGCC AAAGACCAGA AGTGGAAAGA GTGCACTGGA GGCTGAGAGC 
GAGAGTCTGG ATAGCTACAC TGCTGACTCA GACAGCACCT CCAGGAGAGA TTCTCTGGAT 
AAATCTGGAC TCTTCCCAGA ATGGAAGAAG ATGTCCGCTC CCAAATCTCA AGTTGAAAAG 
GAAATTCCAC CAGGGAACCA AAATGCAGTC TGTGGTGATG AGGGTGACAT GGTATTCAAG 
AAGAACACCA AAAAAGTCCT GAGGCCTTCA GAATACACTA AGTCTGTGAT AGACCTTCGC 
CCAGAAGATG TGGCCCAAGA AAGTGGCATC TTGGGAGACA GAAGCAAATC TGTACCAGGC 
CTCAGTGTGG ATATGGAAGA AGAGGAGGAG GAGGAAGAGG ACATTGACCA CCTGGTGAAG 
TTGCACCGCC AGAAACTGGC CAGAGGCAGC ATGCAGAGTG GCTCCTCCAT GAGTACCCTT 
GGCAGCATTA TGAGTATCTA TAGCGAAGCT GGTGATTTTG GGAACATCTC TGTGACGGGC 
AAGATTGCCT TTTCACTGAA GTTTGAGCAG AAAACACAGA CTTTGGTCAT CCATGTCAAG 
GAGTGCCACC AGCTGGCCTA TGCTGATGAA GCCAAGAAGC GTTCTAACCC ATATGTGAAG 
ACTTATCTTC TGCCTGACAA GTCCCGCCAA GGAAAAAGAA AAACCAGCAT CAAGCGGGAC 
ACCATCAATC CACTATATGA TGAGACCTTT CGGTACGAGA TTTCGGAATC TCTTCTGGCT 
CAGAGGACTT TGCAGTTTTC CGTTTGGCAT CATGGTCGTT TTGGCAGAAA CACTTTCCTT 
GGAGAGGCGG AGGTCCACAT GGACTCTTGG AAGCTGGATA AGAAGCTGGA TCACTGCCTC 
CCTTTACATG GAAAGATCAG TACTGAGTCC TCGCCTGGTT TGCCAGCACA CAAAGGCGAG 
TTGGTGGTTT CATTGAAATA CATCCCAGCC TCCAAACTCC CTGTTGGAGG TGACCGGAAA 
AAGAGTAAAG GTGGGGAAGG GGGAGAACTC CAGGTGTGGA TCAAAGAAGC CAAGAACCTG 
ACGGCTGCCA AATCAGGAGG GACTTCAGAC AGCTTTGTCA AGGGATACCT CCTTCCCATG 
AGGAACAAGG CCAGTAAGCG TAAAACTCCT GTGATGAAGA AGACTCTGAG TCCCCACTAC 
AACCATACAT TTGTCTACAA TGGTGTGAGG CTGGAAGATC TACAACACAT GTGCCTGGAA 
CTGACTGTAT GGGACCGGGA GCCCCTGGCC AGCAATGACT TCCTGGGAGG GGTCAGACTG 
GGTGTTGGCA CTGGGATCAG TAATGGCGAA GTGGTGGACT GGATGGATTC GACTGGTGAA 
GAAGTAAGCT TGTGGCAGAA GATGCGACAG TACCCAGGGT CTTGGGCAGA AGGAACTCTG 
CAGCTCCGTT CTTCAATGGT CAAGCAGAAG CTGGGTGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sytl4 ( XM_006528551.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006528614.1 Mus musculus synaptotagmin-like 4 (Sytl4), transcript variant X1, mRNA.

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CloneID	OMu14296
Clone ID Related Accession (Same CDS sequence)	NM_013757.2 , XM_006528551.2 , XM_006528551.3
Accession Version	XM_006528551.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2022bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	synaptotagmin-like protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006528551.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TACTAGACCT CTCTTTTCTG TCCGAGATGG AAAGGGATTT GATCCTCGGT 
GTTCTGCAGA GAGATGAAGA ACTCAGAAAA GCAGATGAGA AAAGGATTCG GCGACTGAAG 
AATGAGCTCC TGGAGATCAA AAGAAAAGGG GCCAAAAGGG GCAGCCAACA CTATAGTGAT 
CGAACCTGTG CCAGGTGCCA GGAGGGCCTG GGCCGCTTAA TTCCCAAAAG CAGCACTTGT 
GTGGGCTGCA ATCATTTAGT GTGCCGAGAA TGCCGAGTTC TGGAAAGCAA TGGCTCTTGG 
AGGTGCAAGG TGTGCTCGAA GGAGATAGAG CTGAAGAAAG CAACTGGAGA TTGGTTTTAT 
GACCAGAAAG TAAATCGCTT TGATTACCGA ACAGGCAGCG AGATAATCAG GATGTCCCTG 
CGCCAGAAGC CTGCAGTGAA TAAAAGAGAG ACAGCGGGGC AGTCCCTCCT TCAGCAGACA 
CAGATGGGTG ATATCTGGCC AGGAAGAAGG ATCATTCAAG AACAACAACA GAGGGAACAA 
AGTGTGCTGT TTGAAGTGCC AAAGACCAGA AGTGGAAAGA GTGCACTGGA GGCTGAGAGC 
GAGAGTCTGG ATAGCTACAC TGCTGACTCA GACAGCACCT CCAGGAGAGA TTCTCTGGAT 
AAATCTGGAC TCTTCCCAGA ATGGAAGAAG ATGTCCGCTC CCAAATCTCA AGTTGAAAAG 
GAAATTCCAC CAGGGAACCA AAATGCAGTC TGTGGTGATG AGGGTGACAT GGTATTCAAG 
AAGAACACCA AAAAAGTCCT GAGGCCTTCA GAATACACTA AGTCTGTGAT AGACCTTCGC 
CCAGAAGATG TGGCCCAAGA AAGTGGCATC TTGGGAGACA GAAGCAAATC TGTACCAGGC 
CTCAGTGTGG ATATGGAAGA AGAGGAGGAG GAGGAAGAGG ACATTGACCA CCTGGTGAAG 
TTGCACCGCC AGAAACTGGC CAGAGGCAGC ATGCAGAGTG GCTCCTCCAT GAGTACCCTT 
GGCAGCATTA TGAGTATCTA TAGCGAAGCT GGTGATTTTG GGAACATCTC TGTGACGGGC 
AAGATTGCCT TTTCACTGAA GTTTGAGCAG AAAACACAGA CTTTGGTCAT CCATGTCAAG 
GAGTGCCACC AGCTGGCCTA TGCTGATGAA GCCAAGAAGC GTTCTAACCC ATATGTGAAG 
ACTTATCTTC TGCCTGACAA GTCCCGCCAA GGAAAAAGAA AAACCAGCAT CAAGCGGGAC 
ACCATCAATC CACTATATGA TGAGACCTTT CGGTACGAGA TTTCGGAATC TCTTCTGGCT 
CAGAGGACTT TGCAGTTTTC CGTTTGGCAT CATGGTCGTT TTGGCAGAAA CACTTTCCTT 
GGAGAGGCGG AGGTCCACAT GGACTCTTGG AAGCTGGATA AGAAGCTGGA TCACTGCCTC 
CCTTTACATG GAAAGATCAG TACTGAGTCC TCGCCTGGTT TGCCAGCACA CAAAGGCGAG 
TTGGTGGTTT CATTGAAATA CATCCCAGCC TCCAAACTCC CTGTTGGAGG TGACCGGAAA 
AAGAGTAAAG GTGGGGAAGG GGGAGAACTC CAGGTGTGGA TCAAAGAAGC CAAGAACCTG 
ACGGCTGCCA AATCAGGAGG GACTTCAGAC AGCTTTGTCA AGGGATACCT CCTTCCCATG 
AGGAACAAGG CCAGTAAGCG TAAAACTCCT GTGATGAAGA AGACTCTGAG TCCCCACTAC 
AACCATACAT TTGTCTACAA TGGTGTGAGG CTGGAAGATC TACAACACAT GTGCCTGGAA 
CTGACTGTAT GGGACCGGGA GCCCCTGGCC AGCAATGACT TCCTGGGAGG GGTCAGACTG 
GGTGTTGGCA CTGGGATCAG TAATGGCGAA GTGGTGGACT GGATGGATTC GACTGGTGAA 
GAAGTAAGCT TGTGGCAGAA GATGCGACAG TACCCAGGGT CTTGGGCAGA AGGAACTCTG 
CAGCTCCGTT CTTCAATGGT CAAGCAGAAG CTGGGTGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006528614.1
CDS	198..2219
Translation	MSEILDLSFLSEMERDLILGVLQRDEELRKADEKRIRRLKNELLEIKRKGAKRGSQHYSDRTCARCQEGLGRLIPKSSTCVGCNHLVCRECRVLESNGSWRCKVCSKEIELKKATGDWFYDQKVNRFDYRTGSEIIRMSLRQKPAVNKRETAGQSLLQQTQMGDIWPGRRIIQEQQQREQSVLFEVPKTRSGKSALEAESESLDSYTADSDSTSRRDSLDKSGLFPEWKKMSAPKSQVEKEIPPGNQNAVCGDEGDMVFKKNTKKVLRPSEYTKSVIDLRPEDVAQESGILGDRSKSVPGLSVDMEEEEEEEEDIDHLVKLHRQKLARGSMQSGSSMSTLGSIMSIYSEAGDFGNISVTGKIAFSLKFEQKTQTLVIHVKECHQLAYADEAKKRSNPYVKTYLLPDKSRQGKRKTSIKRDTINPLYDETFRYEISESLLAQRTLQFSVWHHGRFGRNTFLGEAEVHMDSWKLDKKLDHCLPLHGKISTESSPGLPAHKGELVVSLKYIPASKLPVGGDRKKSKGGEGGELQVWIKEAKNLTAAKSGGTSDSFVKGYLLPMRNKASKRKTPVMKKTLSPHYNHTFVYNGVRLEDLQHMCLELTVWDREPLASNDFLGGVRLGVGTGISNGEVVDWMDSTGEEVSLWQKMRQYPGSWAEGTLQLRSSMVKQKLGV

Target ORF information:

RefSeq Version	XM_006528551.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus synaptotagmin-like 4 (Sytl4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006528551.2

ORF Insert Sequence:

ATGTCGGAGA TACTAGACCT CTCTTTTCTG TCCGAGATGG AAAGGGATTT GATCCTCGGT 
GTTCTGCAGA GAGATGAAGA ACTCAGAAAA GCAGATGAGA AAAGGATTCG GCGACTGAAG 
AATGAGCTCC TGGAGATCAA AAGAAAAGGG GCCAAAAGGG GCAGCCAACA CTATAGTGAT 
CGAACCTGTG CCAGGTGCCA GGAGGGCCTG GGCCGCTTAA TTCCCAAAAG CAGCACTTGT 
GTGGGCTGCA ATCATTTAGT GTGCCGAGAA TGCCGAGTTC TGGAAAGCAA TGGCTCTTGG 
AGGTGCAAGG TGTGCTCGAA GGAGATAGAG CTGAAGAAAG CAACTGGAGA TTGGTTTTAT 
GACCAGAAAG TAAATCGCTT TGATTACCGA ACAGGCAGCG AGATAATCAG GATGTCCCTG 
CGCCAGAAGC CTGCAGTGAA TAAAAGAGAG ACAGCGGGGC AGTCCCTCCT TCAGCAGACA 
CAGATGGGTG ATATCTGGCC AGGAAGAAGG ATCATTCAAG AACAACAACA GAGGGAACAA 
AGTGTGCTGT TTGAAGTGCC AAAGACCAGA AGTGGAAAGA GTGCACTGGA GGCTGAGAGC 
GAGAGTCTGG ATAGCTACAC TGCTGACTCA GACAGCACCT CCAGGAGAGA TTCTCTGGAT 
AAATCTGGAC TCTTCCCAGA ATGGAAGAAG ATGTCCGCTC CCAAATCTCA AGTTGAAAAG 
GAAATTCCAC CAGGGAACCA AAATGCAGTC TGTGGTGATG AGGGTGACAT GGTATTCAAG 
AAGAACACCA AAAAAGTCCT GAGGCCTTCA GAATACACTA AGTCTGTGAT AGACCTTCGC 
CCAGAAGATG TGGCCCAAGA AAGTGGCATC TTGGGAGACA GAAGCAAATC TGTACCAGGC 
CTCAGTGTGG ATATGGAAGA AGAGGAGGAG GAGGAAGAGG ACATTGACCA CCTGGTGAAG 
TTGCACCGCC AGAAACTGGC CAGAGGCAGC ATGCAGAGTG GCTCCTCCAT GAGTACCCTT 
GGCAGCATTA TGAGTATCTA TAGCGAAGCT GGTGATTTTG GGAACATCTC TGTGACGGGC 
AAGATTGCCT TTTCACTGAA GTTTGAGCAG AAAACACAGA CTTTGGTCAT CCATGTCAAG 
GAGTGCCACC AGCTGGCCTA TGCTGATGAA GCCAAGAAGC GTTCTAACCC ATATGTGAAG 
ACTTATCTTC TGCCTGACAA GTCCCGCCAA GGAAAAAGAA AAACCAGCAT CAAGCGGGAC 
ACCATCAATC CACTATATGA TGAGACCTTT CGGTACGAGA TTTCGGAATC TCTTCTGGCT 
CAGAGGACTT TGCAGTTTTC CGTTTGGCAT CATGGTCGTT TTGGCAGAAA CACTTTCCTT 
GGAGAGGCGG AGGTCCACAT GGACTCTTGG AAGCTGGATA AGAAGCTGGA TCACTGCCTC 
CCTTTACATG GAAAGATCAG TACTGAGTCC TCGCCTGGTT TGCCAGCACA CAAAGGCGAG 
TTGGTGGTTT CATTGAAATA CATCCCAGCC TCCAAACTCC CTGTTGGAGG TGACCGGAAA 
AAGAGTAAAG GTGGGGAAGG GGGAGAACTC CAGGTGTGGA TCAAAGAAGC CAAGAACCTG 
ACGGCTGCCA AATCAGGAGG GACTTCAGAC AGCTTTGTCA AGGGATACCT CCTTCCCATG 
AGGAACAAGG CCAGTAAGCG TAAAACTCCT GTGATGAAGA AGACTCTGAG TCCCCACTAC 
AACCATACAT TTGTCTACAA TGGTGTGAGG CTGGAAGATC TACAACACAT GTGCCTGGAA 
CTGACTGTAT GGGACCGGGA GCCCCTGGCC AGCAATGACT TCCTGGGAGG GGTCAGACTG 
GGTGTTGGCA CTGGGATCAG TAATGGCGAA GTGGTGGACT GGATGGATTC GACTGGTGAA 
GAAGTAAGCT TGTGGCAGAA GATGCGACAG TACCCAGGGT CTTGGGCAGA AGGAACTCTG 
CAGCTCCGTT CTTCAATGGT CAAGCAGAAG CTGGGTGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sytl4 ( NM_001290719.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001277648.1 Mus musculus synaptotagmin-like 4 (Sytl4), transcript variant 4, mRNA.

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CloneID	OMu14322
Clone ID Related Accession (Same CDS sequence)	NM_001290719.1
Accession Version	NM_001290719.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1509bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Mus musculus(house mouse)
Product	synaptotagmin-like protein 4 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX510358.4, AB025258.1 and AL691421.9. Transcript Variant: This variant (4) lacks multiple 3' coding exons and its transcription extends past a splice site used in variant 1, resulting in a different 3' coding region and 3' UTR. The encoded isoform (3) has a distinct C-terminus and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC026819.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGGAGA TACTAGACCT CTCTTTTCTG TCCGAGATGG AAAGGGATTT GATCCTCGGT 
GTTCTGCAGA GAGATGAAGA ACTCAGAAAA GCAGATGAGA AAAGGATTCG GCGACTGAAG 
AATGAGCTCC TGGAGATCAA AAGAAAAGGG GCCAAAAGGG GCAGCCAACA CTATAGTGAT 
CGAACCTGTG CCAGGTGCCA GGAGGGCCTG GGCCGCTTAA TTCCCAAAAG CAGCACTTGT 
GTGGGCTGCA ATCATTTAGT GTGCCGAGAA TGCCGAGTTC TGGAAAGCAA TGGCTCTTGG 
AGGTGCAAGG TGTGCTCGAA GGAGATAGAG CTGAAGAAAG CAACTGGAGA TTGGTTTTAT 
GACCAGAAAG TAAATCGCTT TGATTACCGA ACAGGCAGCG AGATAATCAG GATGTCCCTG 
CGCCAGAAGC CTGCAGTGAA TAAAAGAGAG ACAGCGGGGC AGTCCCTCCT TCAGCAGACA 
CAGATGGGTG ATATCTGGCC AGGAAGAAGG ATCATTCAAG AACAACAACA GAGGGAACAA 
AGTGTGCTGT TTGAAGTGCC AAAGACCAGA AGTGGAAAGA GTGCACTGGA GGCTGAGAGC 
GAGAGTCTGG ATAGCTACAC TGCTGACTCA GACAGCACCT CCAGGAGAGA TTCTCTGGAT 
AAATCTGGAC TCTTCCCAGA ATGGAAGAAG ATGTCCGCTC CCAAATCTCA AGTTGAAAAG 
GAAATTCCAC CAGGGAACCA AAATGCAGTC TGTGGTGATG AGGGTGACAT GGTATTCAAG 
AAGAACACCA AAAAAGTCCT GAGGCCTTCA GAATACACTA AGTCTGTGAT AGACCTTCGC 
CCAGAAGATG TGGCCCAAGA AAGTGGCATC TTGGGAGACA GAAGCAAATC TGTACCAGGC 
CTCAGTGTGG ATATGGAAGA AGAGGAGGAG GAGGAAGAGG ACATTGACCA CCTGGTGAAG 
TTGCACCGCC AGAAACTGGC CAGAGGCAGC ATGCAGAGTG GCTCCTCCAT GAGTACCCTT 
GGCAGCATTA TGAGTATCTA TAGCGAAGCT GGTGATTTTG GGAACATCTC TGTGACGGGC 
AAGATTGCCT TTTCACTGAA GTTTGAGCAG AAAACACAGA CTTTGGTCAT CCATGTCAAG 
GAGTGCCACC AGCTGGCCTA TGCTGATGAA GCCAAGAAGC GTTCTAACCC ATATGTGAAG 
ACTTATCTTC TGCCTGACAA GTCCCGCCAA GGAAAAAGAA AAACCAGCAT CAAGCGGGAC 
ACCATCAATC CACTATATGA TGAGACCTTT CGGTACGAGA TTTCGGAATC TCTTCTGGCT 
CAGAGGACTT TGCAGTTTTC CGTTTGGCAT CATGGTCGTT TTGGCAGAAA CACTTTCCTT 
GGAGAGGCGG AGGTCCACAT GGACTCTTGG AAGCTGGATA AGAAGCTGGA TCACTGCCTC 
CCTTTACATG GAAAGGTAGT CTGCTTTAAA GGTCGTTCAC AGCTTGCTTC CCAGGTGAAT 
TGTCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001277648.1
CDS	215..1723
Translation	MSEILDLSFLSEMERDLILGVLQRDEELRKADEKRIRRLKNELLEIKRKGAKRGSQHYSDRTCARCQEGLGRLIPKSSTCVGCNHLVCRECRVLESNGSWRCKVCSKEIELKKATGDWFYDQKVNRFDYRTGSEIIRMSLRQKPAVNKRETAGQSLLQQTQMGDIWPGRRIIQEQQQREQSVLFEVPKTRSGKSALEAESESLDSYTADSDSTSRRDSLDKSGLFPEWKKMSAPKSQVEKEIPPGNQNAVCGDEGDMVFKKNTKKVLRPSEYTKSVIDLRPEDVAQESGILGDRSKSVPGLSVDMEEEEEEEEDIDHLVKLHRQKLARGSMQSGSSMSTLGSIMSIYSEAGDFGNISVTGKIAFSLKFEQKTQTLVIHVKECHQLAYADEAKKRSNPYVKTYLLPDKSRQGKRKTSIKRDTINPLYDETFRYEISESLLAQRTLQFSVWHHGRFGRNTFLGEAEVHMDSWKLDKKLDHCLPLHGKVVCFKGRSQLASQVNCL

Target ORF information:

RefSeq Version	NM_001290719.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus synaptotagmin-like 4 (Sytl4), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001290719.1

ORF Insert Sequence:

ATGTCGGAGA TACTAGACCT CTCTTTTCTG TCCGAGATGG AAAGGGATTT GATCCTCGGT 
GTTCTGCAGA GAGATGAAGA ACTCAGAAAA GCAGATGAGA AAAGGATTCG GCGACTGAAG 
AATGAGCTCC TGGAGATCAA AAGAAAAGGG GCCAAAAGGG GCAGCCAACA CTATAGTGAT 
CGAACCTGTG CCAGGTGCCA GGAGGGCCTG GGCCGCTTAA TTCCCAAAAG CAGCACTTGT 
GTGGGCTGCA ATCATTTAGT GTGCCGAGAA TGCCGAGTTC TGGAAAGCAA TGGCTCTTGG 
AGGTGCAAGG TGTGCTCGAA GGAGATAGAG CTGAAGAAAG CAACTGGAGA TTGGTTTTAT 
GACCAGAAAG TAAATCGCTT TGATTACCGA ACAGGCAGCG AGATAATCAG GATGTCCCTG 
CGCCAGAAGC CTGCAGTGAA TAAAAGAGAG ACAGCGGGGC AGTCCCTCCT TCAGCAGACA 
CAGATGGGTG ATATCTGGCC AGGAAGAAGG ATCATTCAAG AACAACAACA GAGGGAACAA 
AGTGTGCTGT TTGAAGTGCC AAAGACCAGA AGTGGAAAGA GTGCACTGGA GGCTGAGAGC 
GAGAGTCTGG ATAGCTACAC TGCTGACTCA GACAGCACCT CCAGGAGAGA TTCTCTGGAT 
AAATCTGGAC TCTTCCCAGA ATGGAAGAAG ATGTCCGCTC CCAAATCTCA AGTTGAAAAG 
GAAATTCCAC CAGGGAACCA AAATGCAGTC TGTGGTGATG AGGGTGACAT GGTATTCAAG 
AAGAACACCA AAAAAGTCCT GAGGCCTTCA GAATACACTA AGTCTGTGAT AGACCTTCGC 
CCAGAAGATG TGGCCCAAGA AAGTGGCATC TTGGGAGACA GAAGCAAATC TGTACCAGGC 
CTCAGTGTGG ATATGGAAGA AGAGGAGGAG GAGGAAGAGG ACATTGACCA CCTGGTGAAG 
TTGCACCGCC AGAAACTGGC CAGAGGCAGC ATGCAGAGTG GCTCCTCCAT GAGTACCCTT 
GGCAGCATTA TGAGTATCTA TAGCGAAGCT GGTGATTTTG GGAACATCTC TGTGACGGGC 
AAGATTGCCT TTTCACTGAA GTTTGAGCAG AAAACACAGA CTTTGGTCAT CCATGTCAAG 
GAGTGCCACC AGCTGGCCTA TGCTGATGAA GCCAAGAAGC GTTCTAACCC ATATGTGAAG 
ACTTATCTTC TGCCTGACAA GTCCCGCCAA GGAAAAAGAA AAACCAGCAT CAAGCGGGAC 
ACCATCAATC CACTATATGA TGAGACCTTT CGGTACGAGA TTTCGGAATC TCTTCTGGCT 
CAGAGGACTT TGCAGTTTTC CGTTTGGCAT CATGGTCGTT TTGGCAGAAA CACTTTCCTT 
GGAGAGGCGG AGGTCCACAT GGACTCTTGG AAGCTGGATA AGAAGCTGGA TCACTGCCTC 
CCTTTACATG GAAAGGTAGT CTGCTTTAAA GGTCGTTCAC AGCTTGCTTC CCAGGTGAAT 
TGTCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sytl4 ( XM_006528551.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006528614.1 Mus musculus synaptotagmin-like 4 (Sytl4), transcript variant X1, mRNA.

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CloneID	OMu14296
Clone ID Related Accession (Same CDS sequence)	NM_013757.2 , XM_006528551.2 , XM_006528551.3
Accession Version	XM_006528551.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2022bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	synaptotagmin-like protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006528551.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TACTAGACCT CTCTTTTCTG TCCGAGATGG AAAGGGATTT GATCCTCGGT 
GTTCTGCAGA GAGATGAAGA ACTCAGAAAA GCAGATGAGA AAAGGATTCG GCGACTGAAG 
AATGAGCTCC TGGAGATCAA AAGAAAAGGG GCCAAAAGGG GCAGCCAACA CTATAGTGAT 
CGAACCTGTG CCAGGTGCCA GGAGGGCCTG GGCCGCTTAA TTCCCAAAAG CAGCACTTGT 
GTGGGCTGCA ATCATTTAGT GTGCCGAGAA TGCCGAGTTC TGGAAAGCAA TGGCTCTTGG 
AGGTGCAAGG TGTGCTCGAA GGAGATAGAG CTGAAGAAAG CAACTGGAGA TTGGTTTTAT 
GACCAGAAAG TAAATCGCTT TGATTACCGA ACAGGCAGCG AGATAATCAG GATGTCCCTG 
CGCCAGAAGC CTGCAGTGAA TAAAAGAGAG ACAGCGGGGC AGTCCCTCCT TCAGCAGACA 
CAGATGGGTG ATATCTGGCC AGGAAGAAGG ATCATTCAAG AACAACAACA GAGGGAACAA 
AGTGTGCTGT TTGAAGTGCC AAAGACCAGA AGTGGAAAGA GTGCACTGGA GGCTGAGAGC 
GAGAGTCTGG ATAGCTACAC TGCTGACTCA GACAGCACCT CCAGGAGAGA TTCTCTGGAT 
AAATCTGGAC TCTTCCCAGA ATGGAAGAAG ATGTCCGCTC CCAAATCTCA AGTTGAAAAG 
GAAATTCCAC CAGGGAACCA AAATGCAGTC TGTGGTGATG AGGGTGACAT GGTATTCAAG 
AAGAACACCA AAAAAGTCCT GAGGCCTTCA GAATACACTA AGTCTGTGAT AGACCTTCGC 
CCAGAAGATG TGGCCCAAGA AAGTGGCATC TTGGGAGACA GAAGCAAATC TGTACCAGGC 
CTCAGTGTGG ATATGGAAGA AGAGGAGGAG GAGGAAGAGG ACATTGACCA CCTGGTGAAG 
TTGCACCGCC AGAAACTGGC CAGAGGCAGC ATGCAGAGTG GCTCCTCCAT GAGTACCCTT 
GGCAGCATTA TGAGTATCTA TAGCGAAGCT GGTGATTTTG GGAACATCTC TGTGACGGGC 
AAGATTGCCT TTTCACTGAA GTTTGAGCAG AAAACACAGA CTTTGGTCAT CCATGTCAAG 
GAGTGCCACC AGCTGGCCTA TGCTGATGAA GCCAAGAAGC GTTCTAACCC ATATGTGAAG 
ACTTATCTTC TGCCTGACAA GTCCCGCCAA GGAAAAAGAA AAACCAGCAT CAAGCGGGAC 
ACCATCAATC CACTATATGA TGAGACCTTT CGGTACGAGA TTTCGGAATC TCTTCTGGCT 
CAGAGGACTT TGCAGTTTTC CGTTTGGCAT CATGGTCGTT TTGGCAGAAA CACTTTCCTT 
GGAGAGGCGG AGGTCCACAT GGACTCTTGG AAGCTGGATA AGAAGCTGGA TCACTGCCTC 
CCTTTACATG GAAAGATCAG TACTGAGTCC TCGCCTGGTT TGCCAGCACA CAAAGGCGAG 
TTGGTGGTTT CATTGAAATA CATCCCAGCC TCCAAACTCC CTGTTGGAGG TGACCGGAAA 
AAGAGTAAAG GTGGGGAAGG GGGAGAACTC CAGGTGTGGA TCAAAGAAGC CAAGAACCTG 
ACGGCTGCCA AATCAGGAGG GACTTCAGAC AGCTTTGTCA AGGGATACCT CCTTCCCATG 
AGGAACAAGG CCAGTAAGCG TAAAACTCCT GTGATGAAGA AGACTCTGAG TCCCCACTAC 
AACCATACAT TTGTCTACAA TGGTGTGAGG CTGGAAGATC TACAACACAT GTGCCTGGAA 
CTGACTGTAT GGGACCGGGA GCCCCTGGCC AGCAATGACT TCCTGGGAGG GGTCAGACTG 
GGTGTTGGCA CTGGGATCAG TAATGGCGAA GTGGTGGACT GGATGGATTC GACTGGTGAA 
GAAGTAAGCT TGTGGCAGAA GATGCGACAG TACCCAGGGT CTTGGGCAGA AGGAACTCTG 
CAGCTCCGTT CTTCAATGGT CAAGCAGAAG CTGGGTGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006528614.1
CDS	197..2218
Translation	MSEILDLSFLSEMERDLILGVLQRDEELRKADEKRIRRLKNELLEIKRKGAKRGSQHYSDRTCARCQEGLGRLIPKSSTCVGCNHLVCRECRVLESNGSWRCKVCSKEIELKKATGDWFYDQKVNRFDYRTGSEIIRMSLRQKPAVNKRETAGQSLLQQTQMGDIWPGRRIIQEQQQREQSVLFEVPKTRSGKSALEAESESLDSYTADSDSTSRRDSLDKSGLFPEWKKMSAPKSQVEKEIPPGNQNAVCGDEGDMVFKKNTKKVLRPSEYTKSVIDLRPEDVAQESGILGDRSKSVPGLSVDMEEEEEEEEDIDHLVKLHRQKLARGSMQSGSSMSTLGSIMSIYSEAGDFGNISVTGKIAFSLKFEQKTQTLVIHVKECHQLAYADEAKKRSNPYVKTYLLPDKSRQGKRKTSIKRDTINPLYDETFRYEISESLLAQRTLQFSVWHHGRFGRNTFLGEAEVHMDSWKLDKKLDHCLPLHGKISTESSPGLPAHKGELVVSLKYIPASKLPVGGDRKKSKGGEGGELQVWIKEAKNLTAAKSGGTSDSFVKGYLLPMRNKASKRKTPVMKKTLSPHYNHTFVYNGVRLEDLQHMCLELTVWDREPLASNDFLGGVRLGVGTGISNGEVVDWMDSTGEEVSLWQKMRQYPGSWAEGTLQLRSSMVKQKLGV

Target ORF information:

RefSeq Version	XM_006528551.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus synaptotagmin-like 4 (Sytl4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006528551.3

ORF Insert Sequence:

ATGTCGGAGA TACTAGACCT CTCTTTTCTG TCCGAGATGG AAAGGGATTT GATCCTCGGT 
GTTCTGCAGA GAGATGAAGA ACTCAGAAAA GCAGATGAGA AAAGGATTCG GCGACTGAAG 
AATGAGCTCC TGGAGATCAA AAGAAAAGGG GCCAAAAGGG GCAGCCAACA CTATAGTGAT 
CGAACCTGTG CCAGGTGCCA GGAGGGCCTG GGCCGCTTAA TTCCCAAAAG CAGCACTTGT 
GTGGGCTGCA ATCATTTAGT GTGCCGAGAA TGCCGAGTTC TGGAAAGCAA TGGCTCTTGG 
AGGTGCAAGG TGTGCTCGAA GGAGATAGAG CTGAAGAAAG CAACTGGAGA TTGGTTTTAT 
GACCAGAAAG TAAATCGCTT TGATTACCGA ACAGGCAGCG AGATAATCAG GATGTCCCTG 
CGCCAGAAGC CTGCAGTGAA TAAAAGAGAG ACAGCGGGGC AGTCCCTCCT TCAGCAGACA 
CAGATGGGTG ATATCTGGCC AGGAAGAAGG ATCATTCAAG AACAACAACA GAGGGAACAA 
AGTGTGCTGT TTGAAGTGCC AAAGACCAGA AGTGGAAAGA GTGCACTGGA GGCTGAGAGC 
GAGAGTCTGG ATAGCTACAC TGCTGACTCA GACAGCACCT CCAGGAGAGA TTCTCTGGAT 
AAATCTGGAC TCTTCCCAGA ATGGAAGAAG ATGTCCGCTC CCAAATCTCA AGTTGAAAAG 
GAAATTCCAC CAGGGAACCA AAATGCAGTC TGTGGTGATG AGGGTGACAT GGTATTCAAG 
AAGAACACCA AAAAAGTCCT GAGGCCTTCA GAATACACTA AGTCTGTGAT AGACCTTCGC 
CCAGAAGATG TGGCCCAAGA AAGTGGCATC TTGGGAGACA GAAGCAAATC TGTACCAGGC 
CTCAGTGTGG ATATGGAAGA AGAGGAGGAG GAGGAAGAGG ACATTGACCA CCTGGTGAAG 
TTGCACCGCC AGAAACTGGC CAGAGGCAGC ATGCAGAGTG GCTCCTCCAT GAGTACCCTT 
GGCAGCATTA TGAGTATCTA TAGCGAAGCT GGTGATTTTG GGAACATCTC TGTGACGGGC 
AAGATTGCCT TTTCACTGAA GTTTGAGCAG AAAACACAGA CTTTGGTCAT CCATGTCAAG 
GAGTGCCACC AGCTGGCCTA TGCTGATGAA GCCAAGAAGC GTTCTAACCC ATATGTGAAG 
ACTTATCTTC TGCCTGACAA GTCCCGCCAA GGAAAAAGAA AAACCAGCAT CAAGCGGGAC 
ACCATCAATC CACTATATGA TGAGACCTTT CGGTACGAGA TTTCGGAATC TCTTCTGGCT 
CAGAGGACTT TGCAGTTTTC CGTTTGGCAT CATGGTCGTT TTGGCAGAAA CACTTTCCTT 
GGAGAGGCGG AGGTCCACAT GGACTCTTGG AAGCTGGATA AGAAGCTGGA TCACTGCCTC 
CCTTTACATG GAAAGATCAG TACTGAGTCC TCGCCTGGTT TGCCAGCACA CAAAGGCGAG 
TTGGTGGTTT CATTGAAATA CATCCCAGCC TCCAAACTCC CTGTTGGAGG TGACCGGAAA 
AAGAGTAAAG GTGGGGAAGG GGGAGAACTC CAGGTGTGGA TCAAAGAAGC CAAGAACCTG 
ACGGCTGCCA AATCAGGAGG GACTTCAGAC AGCTTTGTCA AGGGATACCT CCTTCCCATG 
AGGAACAAGG CCAGTAAGCG TAAAACTCCT GTGATGAAGA AGACTCTGAG TCCCCACTAC 
AACCATACAT TTGTCTACAA TGGTGTGAGG CTGGAAGATC TACAACACAT GTGCCTGGAA 
CTGACTGTAT GGGACCGGGA GCCCCTGGCC AGCAATGACT TCCTGGGAGG GGTCAGACTG 
GGTGTTGGCA CTGGGATCAG TAATGGCGAA GTGGTGGACT GGATGGATTC GACTGGTGAA 
GAAGTAAGCT TGTGGCAGAA GATGCGACAG TACCCAGGGT CTTGGGCAGA AGGAACTCTG 
CAGCTCCGTT CTTCAATGGT CAAGCAGAAG CTGGGTGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

?-Cell-Specific Mafk Overexpression Impairs Pancreatic Endocrine Cell Development.
PloS one11(2)e0150010(2016)
Abdellatif AM,Oishi H,Itagaki T,Jung Y,Shawki HH,Okita Y,Hasegawa Y,Suzuki H,El-Morsy SE,El-Sayed MA,Shoaib MB,Sugiyama F,Takahashi S

The Rab27a effector exophilin7 promotes fusion of secretory granules that have not been docked to the plasma membrane.
Molecular biology of the cell24(3)319-30(2013 Feb)
Wang H,Ishizaki R,Xu J,Kasai K,Kobayashi E,Gomi H,Izumi T

Modular genetic control of sexually dimorphic behaviors.
Cell148(3)596-607(2012 Feb)
Xu X,Coats JK,Yang CF,Wang A,Ahmed OM,Alvarado M,Izumi T,Shah NM

Loss of granuphilin and loss of syntaxin-1A cause differential effects on insulin granule docking and fusion.
The Journal of biological chemistry286(37)32244-50(2011 Sep)
Wang H,Ishizaki R,Kobayashi E,Fujiwara T,Akagawa K,Izumi T

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Sytl4 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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