Clnk cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Clnk
Entrez Gene ID	27278
Full Name	cytokine-dependent hematopoietic cell linker
Synonyms	MIST
General protein information	Preferred Names cytokine-dependent hematopoietic cell linker Names cytokine-dependent hematopoietic cell linker mast cell immunoreceptor signal transducer
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 B3

mRNA and Protein(s)

mRNA	Protein	Name
XM_006503977.3	XP_006504040.1	cytokine-dependent hematopoietic cell linker isoform X4
XM_006503974.2	XP_006504037.1	cytokine-dependent hematopoietic cell linker isoform X1
NM_013748.3	NP_038776.3	cytokine-dependent hematopoietic cell linker
XM_011240734.1	XP_011239036.1	cytokine-dependent hematopoietic cell linker isoform X2
XM_011240735.1	XP_011239037.1	cytokine-dependent hematopoietic cell linker isoform X3
XM_006503977.4	XP_006504040.1	cytokine-dependent hematopoietic cell linker isoform X4

Pathways from BioSystems

Homologies

Homo sapiens (human)	CLNK	NP_443196.2
Bos taurus (cattle)	CLNK	XP_002688505.1
Mus musculus (house mouse)	Clnk	NP_038776.3
Pan troglodytes (chimpanzee)	CLNK	XP_520687.2
	LOC713238	XP_001098116.2
Rattus norvegicus (Norway rat)	Clnk	XP_006251170.1
Canis lupus familiaris (dog)	CLNK	XP_005618634.1

Gene Ontology
Bibliography

Related articles in PubMed

Clnk plays a role in TNF-alpha-induced cell death in murine fibrosarcoma cell line L929.
Xu M, Cai C, Sun X, Chen W, Li Q, Zhou H
Biochemical and biophysical research communications463(3)275-9(2015 Jul)

Distinct regulatory functions of SLP-76 and MIST in NK cell cytotoxicity and IFN-gamma production.
Hidano S, Sasanuma H, Ohshima K, Seino K, Kumar L, Hayashi K, Hikida M, Kurosaki T, Taniguchi M, Geha RS, Kitamura D, Goitsuka R
International immunology20(3)345-52(2008 Mar)

Dual function for the adaptor MIST in IFN-gamma production by NK and CD4+NKT cells regulated by the Src kinase Fgr.
Sasanuma H, Tatsuno A, Hidano S, Ohshima K, Matsuzaki Y, Hayashi K, Lowell CA, Kitamura D, Goitsuka R
Blood107(9)3647-55(2006 May)

Transcriptional regulation of SLP-76 family hematopoietic cell adaptor MIST/Clnk by STAT5.
Sasanuma H, Tatsuno A, Tsuji K, Hidano S, Morita S, Kitamura T, Kubo M, Kitamura D, Goitsuka R
Biochemical and biophysical research communications321(1)145-53(2004 Aug)

Immune functions in mice lacking Clnk, an SLP-76-related adaptor expressed in a subset of immune cells.
Utting O, Sedgmen BJ, Watts TH, Shi X, Rottapel R, Iulianella A, Lohnes D, Veillette A
Molecular and cellular biology24(13)6067-75(2004 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Clnk is required for TNF-alpha -induced cell death in L929 cells.
Title: Clnk plays a role in TNF-alpha-induced cell death in murine fibrosarcoma cell line L929.

interaction of MIST with Fgr, mediated by the C-terminal proline-rich region of MIST and the SH3 domain of Fgr, was required for the suppression of NK-cell receptor-induced IFN-gamma production
Title: Dual function for the adaptor MIST in IFN-gamma production by NK and CD4+NKT cells regulated by the Src kinase Fgr.

STAT5 is involved in cytokine-mediated up-regulation of MIST gene expression, in collaboration with other lineage-specific transcription factors that promote basal MIST expression in mast cells.
Title: Transcriptional regulation of SLP-76 family hematopoietic cell adaptor MIST/Clnk by STAT5.

Clnk is dispensable for normal differentiation and function of T cells, mast cells, and NK cells. Hence, unlike its relatives, Clnk is not essential for normal immune functions
Title: Immune functions in mice lacking Clnk, an SLP-76-related adaptor expressed in a subset of immune cells.

The following Clnk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Clnk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu69280	XM_011240735.1 Latest version!	Mus musculus cytokine-dependent hematopoietic cell linker (Clnk), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OMu69279	XM_011240734.1 Latest version!	Mus musculus cytokine-dependent hematopoietic cell linker (Clnk), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu09882	NM_013748.3 Latest version!	Mus musculus cytokine-dependent hematopoietic cell linker (Clnk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OMu09882	XM_006503974.2 Latest version!	Mus musculus cytokine-dependent hematopoietic cell linker (Clnk), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OMu35104	XM_006503977.4 Latest version!	Mus musculus cytokine-dependent hematopoietic cell linker (Clnk), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OMu35104	XM_006503977.3	Mus musculus cytokine-dependent hematopoietic cell linker (Clnk), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Clnk ( XM_011240735.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011239037.1 Mus musculus cytokine-dependent hematopoietic cell linker (Clnk), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu69280
Clone ID Related Accession (Same CDS sequence)	XM_011240735.1
Accession Version	XM_011240735.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1062bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	cytokine-dependent hematopoietic cell linker isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATTT TACCAGCCAG ACCTATCCAG GAATCGGAAT ACGCAGATAC ACGCTATTTC 
CAGGATACGA TGGAGGCTCC CCTTCTGTTA CCTCCCAAGG CTTCTGTCTC CACTGAGAGA 
CAAACCAGGG ATGTGAGGAT GACACATCTG GAAGAAGTGG ACAAGCCTAC CTTCAAGGAT 
GTCAGAAGCC AACGCTTTAA AGGATTCAAA TACACAAAAA TAAACAAGAC TCCTTTGCCA 
CCTCCTCGGC CTGCTATCAC TCTCCCCAAG AAGTACCAAC CCTTACCCCC AGCACCACCA 
GAGGAGAGCA GTGCATACTT CGCTCCAAAG CCCACCTTTC CAGAAGTCCA GAGGGGGCCC 
AGGCAGAGGA GTGCAAAAGA CTTCAGTAGG GTCCTTGGAG CAGAAGAAGA ATCTCACCAC 
CAGACAAAGC CAGAATCTTC TTGCCCATCA TCAAACCAAA ACACACAGAA GAGTCCACCT 
GCCATTGCCA GCTCTTCCTA CATGCCAGGA AAGCACAGTA TACAAGCCAG AGACCATACA 
GGTAGCATGC AGCACTGTCC TGCTCAGAGA TGCCAAGCTG CAGCCAGCCA CAGCCCTCGA 
ATGCTGCCCT ATGAAAACAC AAACTCGGAG AAACCTGACC CCACAAAGCC TGATGAGAAG 
GATGTCTGGC AGAATGAATG GTACATTGGA GAATACAGTC GCCAGGCAGT GGAAGATGTG 
TTAATGAAAG AGAACAAGGA TGGTACTTTT TTGGTCCGAG ACTGCTCTAC AAAATCCAAG 
GCAGAACCAT ATGTTTTGGT GGTGTTTTAT GGGAACAAGG TCTACAATGT GAAAATCCGT 
TTCCTCGAGA GCAATCAACA GTTTGCCCTG GGCACAGGAC TACGAGGAAA TGAGATGTTT 
GATTCTGTGG AAGACATCAT TGAACACTAC ACATATTTTC CCATTCTGCT AATAGATGGG 
AAAGACAAGG CTGCACGCAG GAAACAGTGC TACCTCACCC AGCCACTGCC TCTCGCCAGG 
CTCCTTCTCA CTCAGTACTC CAGCCAGGCA CTTCATGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011239037.1
CDS	144..1205
Translation	MKILPARPIQESEYADTRYFQDTMEAPLLLPPKASVSTERQTRDVRMTHLEEVDKPTFKDVRSQRFKGFKYTKINKTPLPPPRPAITLPKKYQPLPPAPPEESSAYFAPKPTFPEVQRGPRQRSAKDFSRVLGAEEESHHQTKPESSCPSSNQNTQKSPPAIASSSYMPGKHSIQARDHTGSMQHCPAQRCQAAASHSPRMLPYENTNSEKPDPTKPDEKDVWQNEWYIGEYSRQAVEDVLMKENKDGTFLVRDCSTKSKAEPYVLVVFYGNKVYNVKIRFLESNQQFALGTGLRGNEMFDSVEDIIEHYTYFPILLIDGKDKAARRKQCYLTQPLPLARLLLTQYSSQALHE

Target ORF information:

RefSeq Version	XM_011240735.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus cytokine-dependent hematopoietic cell linker (Clnk), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011240735.1

ORF Insert Sequence:

ATGAAAATTT TACCAGCCAG ACCTATCCAG GAATCGGAAT ACGCAGATAC ACGCTATTTC 
CAGGATACGA TGGAGGCTCC CCTTCTGTTA CCTCCCAAGG CTTCTGTCTC CACTGAGAGA 
CAAACCAGGG ATGTGAGGAT GACACATCTG GAAGAAGTGG ACAAGCCTAC CTTCAAGGAT 
GTCAGAAGCC AACGCTTTAA AGGATTCAAA TACACAAAAA TAAACAAGAC TCCTTTGCCA 
CCTCCTCGGC CTGCTATCAC TCTCCCCAAG AAGTACCAAC CCTTACCCCC AGCACCACCA 
GAGGAGAGCA GTGCATACTT CGCTCCAAAG CCCACCTTTC CAGAAGTCCA GAGGGGGCCC 
AGGCAGAGGA GTGCAAAAGA CTTCAGTAGG GTCCTTGGAG CAGAAGAAGA ATCTCACCAC 
CAGACAAAGC CAGAATCTTC TTGCCCATCA TCAAACCAAA ACACACAGAA GAGTCCACCT 
GCCATTGCCA GCTCTTCCTA CATGCCAGGA AAGCACAGTA TACAAGCCAG AGACCATACA 
GGTAGCATGC AGCACTGTCC TGCTCAGAGA TGCCAAGCTG CAGCCAGCCA CAGCCCTCGA 
ATGCTGCCCT ATGAAAACAC AAACTCGGAG AAACCTGACC CCACAAAGCC TGATGAGAAG 
GATGTCTGGC AGAATGAATG GTACATTGGA GAATACAGTC GCCAGGCAGT GGAAGATGTG 
TTAATGAAAG AGAACAAGGA TGGTACTTTT TTGGTCCGAG ACTGCTCTAC AAAATCCAAG 
GCAGAACCAT ATGTTTTGGT GGTGTTTTAT GGGAACAAGG TCTACAATGT GAAAATCCGT 
TTCCTCGAGA GCAATCAACA GTTTGCCCTG GGCACAGGAC TACGAGGAAA TGAGATGTTT 
GATTCTGTGG AAGACATCAT TGAACACTAC ACATATTTTC CCATTCTGCT AATAGATGGG 
AAAGACAAGG CTGCACGCAG GAAACAGTGC TACCTCACCC AGCCACTGCC TCTCGCCAGG 
CTCCTTCTCA CTCAGTACTC CAGCCAGGCA CTTCATGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Clnk ( XM_006503977.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006504040.1 Mus musculus cytokine-dependent hematopoietic cell linker (Clnk), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu35104
Clone ID Related Accession (Same CDS sequence)	XM_006503977.3 , XM_006503977.4
Accession Version	XM_006503977.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 708bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	cytokine-dependent hematopoietic cell linker isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006503977.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATGC AGAGGAGTGC AAAAGACTTC AGTAGGGTCC TTGGAGCAGA AGAAGAATCT 
CACCACCAGA CAAAGCCAGA ATCTTCTTGC CCATCATCAA ACCAAAACAC ACAGAAGAGT 
CCACCTGCCA TTGCCAGCTC TTCCTACATG CCAGGAAAGC ACAGTATACA AGCCAGAGAC 
CATACAGGTA GCATGCAGCA CTGTCCTGCT CAGAGATGCC AAGCTGCAGC CAGCCACAGC 
CCTCGAATGC TGCCCTATGA AAACACAAAC TCGGAGAAAC CTGACCCCAC AAAGCCTGAT 
GAGAAGGATG TCTGGCAGAA TGAATGGTAC ATTGGAGAAT ACAGTCGCCA GGCAGTGGAA 
GATGTGTTAA TGAAAGAGAA CAAGGATGGT ACTTTTTTGG TCCGAGACTG CTCTACAAAA 
TCCAAGGCAG AACCATATGT TTTGGTGGTG TTTTATGGGA ACAAGGTCTA CAATGTGAAA 
ATCCGTTTCC TCGAGAGCAA TCAACAGTTT GCCCTGGGCA CAGGACTACG AGGAAATGAG 
ATGTTTGATT CTGTGGAAGA CATCATTGAA CACTACACAT ATTTTCCCAT TCTGCTAATA 
GATGGGAAAG ACAAGGCTGC ACGCAGGAAA CAGTGCTACC TCACCCAGCC ACTGCCTCTC 
GCCAGGCTCC TTCTCACTCA GTACTCCAGC CAGGCACTTC ATGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006504040.1
CDS	130..837
Translation	MKMQRSAKDFSRVLGAEEESHHQTKPESSCPSSNQNTQKSPPAIASSSYMPGKHSIQARDHTGSMQHCPAQRCQAAASHSPRMLPYENTNSEKPDPTKPDEKDVWQNEWYIGEYSRQAVEDVLMKENKDGTFLVRDCSTKSKAEPYVLVVFYGNKVYNVKIRFLESNQQFALGTGLRGNEMFDSVEDIIEHYTYFPILLIDGKDKAARRKQCYLTQPLPLARLLLTQYSSQALHE

Target ORF information:

RefSeq Version	XM_006503977.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus cytokine-dependent hematopoietic cell linker (Clnk), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006503977.3

ORF Insert Sequence:

ATGAAAATGC AGAGGAGTGC AAAAGACTTC AGTAGGGTCC TTGGAGCAGA AGAAGAATCT 
CACCACCAGA CAAAGCCAGA ATCTTCTTGC CCATCATCAA ACCAAAACAC ACAGAAGAGT 
CCACCTGCCA TTGCCAGCTC TTCCTACATG CCAGGAAAGC ACAGTATACA AGCCAGAGAC 
CATACAGGTA GCATGCAGCA CTGTCCTGCT CAGAGATGCC AAGCTGCAGC CAGCCACAGC 
CCTCGAATGC TGCCCTATGA AAACACAAAC TCGGAGAAAC CTGACCCCAC AAAGCCTGAT 
GAGAAGGATG TCTGGCAGAA TGAATGGTAC ATTGGAGAAT ACAGTCGCCA GGCAGTGGAA 
GATGTGTTAA TGAAAGAGAA CAAGGATGGT ACTTTTTTGG TCCGAGACTG CTCTACAAAA 
TCCAAGGCAG AACCATATGT TTTGGTGGTG TTTTATGGGA ACAAGGTCTA CAATGTGAAA 
ATCCGTTTCC TCGAGAGCAA TCAACAGTTT GCCCTGGGCA CAGGACTACG AGGAAATGAG 
ATGTTTGATT CTGTGGAAGA CATCATTGAA CACTACACAT ATTTTCCCAT TCTGCTAATA 
GATGGGAAAG ACAAGGCTGC ACGCAGGAAA CAGTGCTACC TCACCCAGCC ACTGCCTCTC 
GCCAGGCTCC TTCTCACTCA GTACTCCAGC CAGGCACTTC ATGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Clnk ( XM_011240734.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011239036.1 Mus musculus cytokine-dependent hematopoietic cell linker (Clnk), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu69279
Clone ID Related Accession (Same CDS sequence)	XM_011240734.1
Accession Version	XM_011240734.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1236bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	cytokine-dependent hematopoietic cell linker isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCAGCC AGTCATGGCC AAGCCTCAGC AGTGCCAAAG GGCGGTGTCG AGCGGTTCTG 
GAACCACTTC CGGATCACAG AAGGAACTTG GCTGGGGTCC CAGGTGGAGA AAAATGCAAC 
AGTAACAACG ACTACGAAGA TCCTGAGTTC CAGCTGCTGA AGGCATGGCC ATCAATGAAA 
ATTTTACCAG CCAGACCTAT CCAGGAATCG GAATACGCAG ATACACGCTA TTTCCAGGAT 
ACGATGGAGG CTCCCCTTCT GTTACCTCCC AAGGCTTCTG TCTCCACTGA GAGACAAACC 
AGGGATGTGA GGATGACACA TCTGGAAGAA GTGGACAAGC CTACCTTCAA GGATGTCAGA 
AGCCAACGCT TTAAAGGATT CAAATACACA AAAATAAACA AGACTCCTTT GCCACCTCCT 
CGGCCTGCTA TCACTCTCCC CAAGAAGTAC CAACCCTTAC CCCCAGCACC ACCAGAGGAG 
AGCAGTGCAT ACTTCGCTCC AAAGCCCACC TTTCCAGAAG TCCAGAGGGG GCCCAGGCAG 
AGGAGTGCAA AAGACTTCAG TAGGGTCCTT GGAGCAGAAG AAGAATCTCA CCACCAGACA 
AAGCCAGAAT CTTCTTGCCC ATCATCAAAC CAAAACACAC AGAAGAGTCC ACCTGCCATT 
GCCAGCTCTT CCTACATGCC AGGAAAGCAC AGTATACAAG CCAGAGACCA TACAGGTAGC 
ATGCAGCACT GTCCTGCTCA GAGATGCCAA GCTGCAGCCA GCCACAGCCC TCGAATGCTG 
CCCTATGAAA ACACAAACTC GGAGAAACCT GACCCCACAA AGCCTGATGA GAAGGATGTC 
TGGCAGAATG AATGGTACAT TGGAGAATAC AGTCGCCAGG CAGTGGAAGA TGTGTTAATG 
AAAGAGAACA AGGATGGTAC TTTTTTGGTC CGAGACTGCT CTACAAAATC CAAGGCAGAA 
CCATATGTTT TGGTGGTGTT TTATGGGAAC AAGGTCTACA ATGTGAAAAT CCGTTTCCTC 
GAGAGCAATC AACAGTTTGC CCTGGGCACA GGACTACGAG GAAATGAGAT GTTTGATTCT 
GTGGAAGACA TCATTGAACA CTACACATAT TTTCCCATTC TGCTAATAGA TGGGAAAGAC 
AAGGCTGCAC GCAGGAAACA GTGCTACCTC ACCCAGCCAC TGCCTCTCGC CAGGCTCCTT 
CTCACTCAGT ACTCCAGCCA GGCACTTCAT GAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011239036.1
CDS	300..1535
Translation	MTSQSWPSLSSAKGRCRAVLEPLPDHRRNLAGVPGGEKCNSNNDYEDPEFQLLKAWPSMKILPARPIQESEYADTRYFQDTMEAPLLLPPKASVSTERQTRDVRMTHLEEVDKPTFKDVRSQRFKGFKYTKINKTPLPPPRPAITLPKKYQPLPPAPPEESSAYFAPKPTFPEVQRGPRQRSAKDFSRVLGAEEESHHQTKPESSCPSSNQNTQKSPPAIASSSYMPGKHSIQARDHTGSMQHCPAQRCQAAASHSPRMLPYENTNSEKPDPTKPDEKDVWQNEWYIGEYSRQAVEDVLMKENKDGTFLVRDCSTKSKAEPYVLVVFYGNKVYNVKIRFLESNQQFALGTGLRGNEMFDSVEDIIEHYTYFPILLIDGKDKAARRKQCYLTQPLPLARLLLTQYSSQALHE

Target ORF information:

RefSeq Version	XM_011240734.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus cytokine-dependent hematopoietic cell linker (Clnk), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011240734.1

ORF Insert Sequence:

ATGACCAGCC AGTCATGGCC AAGCCTCAGC AGTGCCAAAG GGCGGTGTCG AGCGGTTCTG 
GAACCACTTC CGGATCACAG AAGGAACTTG GCTGGGGTCC CAGGTGGAGA AAAATGCAAC 
AGTAACAACG ACTACGAAGA TCCTGAGTTC CAGCTGCTGA AGGCATGGCC ATCAATGAAA 
ATTTTACCAG CCAGACCTAT CCAGGAATCG GAATACGCAG ATACACGCTA TTTCCAGGAT 
ACGATGGAGG CTCCCCTTCT GTTACCTCCC AAGGCTTCTG TCTCCACTGA GAGACAAACC 
AGGGATGTGA GGATGACACA TCTGGAAGAA GTGGACAAGC CTACCTTCAA GGATGTCAGA 
AGCCAACGCT TTAAAGGATT CAAATACACA AAAATAAACA AGACTCCTTT GCCACCTCCT 
CGGCCTGCTA TCACTCTCCC CAAGAAGTAC CAACCCTTAC CCCCAGCACC ACCAGAGGAG 
AGCAGTGCAT ACTTCGCTCC AAAGCCCACC TTTCCAGAAG TCCAGAGGGG GCCCAGGCAG 
AGGAGTGCAA AAGACTTCAG TAGGGTCCTT GGAGCAGAAG AAGAATCTCA CCACCAGACA 
AAGCCAGAAT CTTCTTGCCC ATCATCAAAC CAAAACACAC AGAAGAGTCC ACCTGCCATT 
GCCAGCTCTT CCTACATGCC AGGAAAGCAC AGTATACAAG CCAGAGACCA TACAGGTAGC 
ATGCAGCACT GTCCTGCTCA GAGATGCCAA GCTGCAGCCA GCCACAGCCC TCGAATGCTG 
CCCTATGAAA ACACAAACTC GGAGAAACCT GACCCCACAA AGCCTGATGA GAAGGATGTC 
TGGCAGAATG AATGGTACAT TGGAGAATAC AGTCGCCAGG CAGTGGAAGA TGTGTTAATG 
AAAGAGAACA AGGATGGTAC TTTTTTGGTC CGAGACTGCT CTACAAAATC CAAGGCAGAA 
CCATATGTTT TGGTGGTGTT TTATGGGAAC AAGGTCTACA ATGTGAAAAT CCGTTTCCTC 
GAGAGCAATC AACAGTTTGC CCTGGGCACA GGACTACGAG GAAATGAGAT GTTTGATTCT 
GTGGAAGACA TCATTGAACA CTACACATAT TTTCCCATTC TGCTAATAGA TGGGAAAGAC 
AAGGCTGCAC GCAGGAAACA GTGCTACCTC ACCCAGCCAC TGCCTCTCGC CAGGCTCCTT 
CTCACTCAGT ACTCCAGCCA GGCACTTCAT GAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Clnk ( NM_013748.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_038776.3 Mus musculus cytokine-dependent hematopoietic cell linker (Clnk), mRNA.

Price & Availability↑

CloneID	OMu09882
Clone ID Related Accession (Same CDS sequence)	XM_006503974.2 , NM_013748.3
Accession Version	NM_013748.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1308bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Mus musculus(house mouse)
Product	cytokine-dependent hematopoietic cell linker
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF187819.1. On Jun 3, 2007 this sequence version replaced NM_013748.2. ##Evidence-Data-START## Transcript exon combination :: AF187819.1, AB021220.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGACCAGCC AGGGCAATAA AAGGACAACG AAAGAAGGAT TCGGTGATCT GAGATTCCAG 
AACGTCTCTC TGCTGAAAAA TAGGTCATGG CCAAGCCTCA GCAGTGCCAA AGGGCGGTGT 
CGAGCGGTTC TGGAACCACT TCCGGATCAC AGAAGGAACT TGGCTGGGGT CCCAGGTGGA 
GAAAAATGCA ACAGTAACAA CGACTACGAA GATCCTGAGT TCCAGCTGCT GAAGGCATGG 
CCATCAATGA AAATTTTACC AGCCAGACCT ATCCAGGAAT CGGAATACGC AGATACACGC 
TATTTCCAGG ATACGATGGA GGCTCCCCTT CTGTTACCTC CCAAGGCTTC TGTCTCCACT 
GAGAGACAAA CCAGGGATGT GAGGATGACA CATCTGGAAG AAGTGGACAA GCCTACCTTC 
AAGGATGTCA GAAGCCAACG CTTTAAAGGA TTCAAATACA CAAAAATAAA CAAGACTCCT 
TTGCCACCTC CTCGGCCTGC TATCACTCTC CCCAAGAAGT ACCAACCCTT ACCCCCAGCA 
CCACCAGAGG AGAGCAGTGC ATACTTCGCT CCAAAGCCCA CCTTTCCAGA AGTCCAGAGG 
GGGCCCAGGC AGAGGAGTGC AAAAGACTTC AGTAGGGTCC TTGGAGCAGA AGAAGAATCT 
CACCACCAGA CAAAGCCAGA ATCTTCTTGC CCATCATCAA ACCAAAACAC ACAGAAGAGT 
CCACCTGCCA TTGCCAGCTC TTCCTACATG CCAGGAAAGC ACAGTATACA AGCCAGAGAC 
CATACAGGTA GCATGCAGCA CTGTCCTGCT CAGAGATGCC AAGCTGCAGC CAGCCACAGC 
CCTCGAATGC TGCCCTATGA AAACACAAAC TCGGAGAAAC CTGACCCCAC AAAGCCTGAT 
GAGAAGGATG TCTGGCAGAA TGAATGGTAC ATTGGAGAAT ACAGTCGCCA GGCAGTGGAA 
GATGTGTTAA TGAAAGAGAA CAAGGATGGT ACTTTTTTGG TCCGAGACTG CTCTACAAAA 
TCCAAGGCAG AACCATATGT TTTGGTGGTG TTTTATGGGA ACAAGGTCTA CAATGTGAAA 
ATCCGTTTCC TCGAGAGCAA TCAACAGTTT GCCCTGGGCA CAGGACTACG AGGAAATGAG 
ATGTTTGATT CTGTGGAAGA CATCATTGAA CACTACACAT ATTTTCCCAT TCTGCTAATA 
GATGGGAAAG ACAAGGCTGC ACGCAGGAAA CAGTGCTACC TCACCCAGCC ACTGCCTCTC 
GCCAGGCTCC TTCTCACTCA GTACTCCAGC CAGGCACTTC ATGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038776.3
CDS	135..1442
Translation	MTSQGNKRTTKEGFGDLRFQNVSLLKNRSWPSLSSAKGRCRAVLEPLPDHRRNLAGVPGGEKCNSNNDYEDPEFQLLKAWPSMKILPARPIQESEYADTRYFQDTMEAPLLLPPKASVSTERQTRDVRMTHLEEVDKPTFKDVRSQRFKGFKYTKINKTPLPPPRPAITLPKKYQPLPPAPPEESSAYFAPKPTFPEVQRGPRQRSAKDFSRVLGAEEESHHQTKPESSCPSSNQNTQKSPPAIASSSYMPGKHSIQARDHTGSMQHCPAQRCQAAASHSPRMLPYENTNSEKPDPTKPDEKDVWQNEWYIGEYSRQAVEDVLMKENKDGTFLVRDCSTKSKAEPYVLVVFYGNKVYNVKIRFLESNQQFALGTGLRGNEMFDSVEDIIEHYTYFPILLIDGKDKAARRKQCYLTQPLPLARLLLTQYSSQALHE

Target ORF information:

RefSeq Version	NM_013748.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus cytokine-dependent hematopoietic cell linker (Clnk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013748.3

ORF Insert Sequence:

ATGACCAGCC AGGGCAATAA AAGGACAACG AAAGAAGGAT TCGGTGATCT GAGATTCCAG 
AACGTCTCTC TGCTGAAAAA TAGGTCATGG CCAAGCCTCA GCAGTGCCAA AGGGCGGTGT 
CGAGCGGTTC TGGAACCACT TCCGGATCAC AGAAGGAACT TGGCTGGGGT CCCAGGTGGA 
GAAAAATGCA ACAGTAACAA CGACTACGAA GATCCTGAGT TCCAGCTGCT GAAGGCATGG 
CCATCAATGA AAATTTTACC AGCCAGACCT ATCCAGGAAT CGGAATACGC AGATACACGC 
TATTTCCAGG ATACGATGGA GGCTCCCCTT CTGTTACCTC CCAAGGCTTC TGTCTCCACT 
GAGAGACAAA CCAGGGATGT GAGGATGACA CATCTGGAAG AAGTGGACAA GCCTACCTTC 
AAGGATGTCA GAAGCCAACG CTTTAAAGGA TTCAAATACA CAAAAATAAA CAAGACTCCT 
TTGCCACCTC CTCGGCCTGC TATCACTCTC CCCAAGAAGT ACCAACCCTT ACCCCCAGCA 
CCACCAGAGG AGAGCAGTGC ATACTTCGCT CCAAAGCCCA CCTTTCCAGA AGTCCAGAGG 
GGGCCCAGGC AGAGGAGTGC AAAAGACTTC AGTAGGGTCC TTGGAGCAGA AGAAGAATCT 
CACCACCAGA CAAAGCCAGA ATCTTCTTGC CCATCATCAA ACCAAAACAC ACAGAAGAGT 
CCACCTGCCA TTGCCAGCTC TTCCTACATG CCAGGAAAGC ACAGTATACA AGCCAGAGAC 
CATACAGGTA GCATGCAGCA CTGTCCTGCT CAGAGATGCC AAGCTGCAGC CAGCCACAGC 
CCTCGAATGC TGCCCTATGA AAACACAAAC TCGGAGAAAC CTGACCCCAC AAAGCCTGAT 
GAGAAGGATG TCTGGCAGAA TGAATGGTAC ATTGGAGAAT ACAGTCGCCA GGCAGTGGAA 
GATGTGTTAA TGAAAGAGAA CAAGGATGGT ACTTTTTTGG TCCGAGACTG CTCTACAAAA 
TCCAAGGCAG AACCATATGT TTTGGTGGTG TTTTATGGGA ACAAGGTCTA CAATGTGAAA 
ATCCGTTTCC TCGAGAGCAA TCAACAGTTT GCCCTGGGCA CAGGACTACG AGGAAATGAG 
ATGTTTGATT CTGTGGAAGA CATCATTGAA CACTACACAT ATTTTCCCAT TCTGCTAATA 
GATGGGAAAG ACAAGGCTGC ACGCAGGAAA CAGTGCTACC TCACCCAGCC ACTGCCTCTC 
GCCAGGCTCC TTCTCACTCA GTACTCCAGC CAGGCACTTC ATGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Clnk ( XM_006503974.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006504037.1 Mus musculus cytokine-dependent hematopoietic cell linker (Clnk), transcript variant X1, mRNA.

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CloneID	OMu09882
Clone ID Related Accession (Same CDS sequence)	XM_006503974.2 , NM_013748.3
Accession Version	XM_006503974.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1308bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	cytokine-dependent hematopoietic cell linker isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006503974.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCAGCC AGGGCAATAA AAGGACAACG AAAGAAGGAT TCGGTGATCT GAGATTCCAG 
AACGTCTCTC TGCTGAAAAA TAGGTCATGG CCAAGCCTCA GCAGTGCCAA AGGGCGGTGT 
CGAGCGGTTC TGGAACCACT TCCGGATCAC AGAAGGAACT TGGCTGGGGT CCCAGGTGGA 
GAAAAATGCA ACAGTAACAA CGACTACGAA GATCCTGAGT TCCAGCTGCT GAAGGCATGG 
CCATCAATGA AAATTTTACC AGCCAGACCT ATCCAGGAAT CGGAATACGC AGATACACGC 
TATTTCCAGG ATACGATGGA GGCTCCCCTT CTGTTACCTC CCAAGGCTTC TGTCTCCACT 
GAGAGACAAA CCAGGGATGT GAGGATGACA CATCTGGAAG AAGTGGACAA GCCTACCTTC 
AAGGATGTCA GAAGCCAACG CTTTAAAGGA TTCAAATACA CAAAAATAAA CAAGACTCCT 
TTGCCACCTC CTCGGCCTGC TATCACTCTC CCCAAGAAGT ACCAACCCTT ACCCCCAGCA 
CCACCAGAGG AGAGCAGTGC ATACTTCGCT CCAAAGCCCA CCTTTCCAGA AGTCCAGAGG 
GGGCCCAGGC AGAGGAGTGC AAAAGACTTC AGTAGGGTCC TTGGAGCAGA AGAAGAATCT 
CACCACCAGA CAAAGCCAGA ATCTTCTTGC CCATCATCAA ACCAAAACAC ACAGAAGAGT 
CCACCTGCCA TTGCCAGCTC TTCCTACATG CCAGGAAAGC ACAGTATACA AGCCAGAGAC 
CATACAGGTA GCATGCAGCA CTGTCCTGCT CAGAGATGCC AAGCTGCAGC CAGCCACAGC 
CCTCGAATGC TGCCCTATGA AAACACAAAC TCGGAGAAAC CTGACCCCAC AAAGCCTGAT 
GAGAAGGATG TCTGGCAGAA TGAATGGTAC ATTGGAGAAT ACAGTCGCCA GGCAGTGGAA 
GATGTGTTAA TGAAAGAGAA CAAGGATGGT ACTTTTTTGG TCCGAGACTG CTCTACAAAA 
TCCAAGGCAG AACCATATGT TTTGGTGGTG TTTTATGGGA ACAAGGTCTA CAATGTGAAA 
ATCCGTTTCC TCGAGAGCAA TCAACAGTTT GCCCTGGGCA CAGGACTACG AGGAAATGAG 
ATGTTTGATT CTGTGGAAGA CATCATTGAA CACTACACAT ATTTTCCCAT TCTGCTAATA 
GATGGGAAAG ACAAGGCTGC ACGCAGGAAA CAGTGCTACC TCACCCAGCC ACTGCCTCTC 
GCCAGGCTCC TTCTCACTCA GTACTCCAGC CAGGCACTTC ATGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006504037.1
CDS	143..1450
Translation	MTSQGNKRTTKEGFGDLRFQNVSLLKNRSWPSLSSAKGRCRAVLEPLPDHRRNLAGVPGGEKCNSNNDYEDPEFQLLKAWPSMKILPARPIQESEYADTRYFQDTMEAPLLLPPKASVSTERQTRDVRMTHLEEVDKPTFKDVRSQRFKGFKYTKINKTPLPPPRPAITLPKKYQPLPPAPPEESSAYFAPKPTFPEVQRGPRQRSAKDFSRVLGAEEESHHQTKPESSCPSSNQNTQKSPPAIASSSYMPGKHSIQARDHTGSMQHCPAQRCQAAASHSPRMLPYENTNSEKPDPTKPDEKDVWQNEWYIGEYSRQAVEDVLMKENKDGTFLVRDCSTKSKAEPYVLVVFYGNKVYNVKIRFLESNQQFALGTGLRGNEMFDSVEDIIEHYTYFPILLIDGKDKAARRKQCYLTQPLPLARLLLTQYSSQALHE

Target ORF information:

RefSeq Version	XM_006503974.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus cytokine-dependent hematopoietic cell linker (Clnk), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006503974.2

ORF Insert Sequence:

ATGACCAGCC AGGGCAATAA AAGGACAACG AAAGAAGGAT TCGGTGATCT GAGATTCCAG 
AACGTCTCTC TGCTGAAAAA TAGGTCATGG CCAAGCCTCA GCAGTGCCAA AGGGCGGTGT 
CGAGCGGTTC TGGAACCACT TCCGGATCAC AGAAGGAACT TGGCTGGGGT CCCAGGTGGA 
GAAAAATGCA ACAGTAACAA CGACTACGAA GATCCTGAGT TCCAGCTGCT GAAGGCATGG 
CCATCAATGA AAATTTTACC AGCCAGACCT ATCCAGGAAT CGGAATACGC AGATACACGC 
TATTTCCAGG ATACGATGGA GGCTCCCCTT CTGTTACCTC CCAAGGCTTC TGTCTCCACT 
GAGAGACAAA CCAGGGATGT GAGGATGACA CATCTGGAAG AAGTGGACAA GCCTACCTTC 
AAGGATGTCA GAAGCCAACG CTTTAAAGGA TTCAAATACA CAAAAATAAA CAAGACTCCT 
TTGCCACCTC CTCGGCCTGC TATCACTCTC CCCAAGAAGT ACCAACCCTT ACCCCCAGCA 
CCACCAGAGG AGAGCAGTGC ATACTTCGCT CCAAAGCCCA CCTTTCCAGA AGTCCAGAGG 
GGGCCCAGGC AGAGGAGTGC AAAAGACTTC AGTAGGGTCC TTGGAGCAGA AGAAGAATCT 
CACCACCAGA CAAAGCCAGA ATCTTCTTGC CCATCATCAA ACCAAAACAC ACAGAAGAGT 
CCACCTGCCA TTGCCAGCTC TTCCTACATG CCAGGAAAGC ACAGTATACA AGCCAGAGAC 
CATACAGGTA GCATGCAGCA CTGTCCTGCT CAGAGATGCC AAGCTGCAGC CAGCCACAGC 
CCTCGAATGC TGCCCTATGA AAACACAAAC TCGGAGAAAC CTGACCCCAC AAAGCCTGAT 
GAGAAGGATG TCTGGCAGAA TGAATGGTAC ATTGGAGAAT ACAGTCGCCA GGCAGTGGAA 
GATGTGTTAA TGAAAGAGAA CAAGGATGGT ACTTTTTTGG TCCGAGACTG CTCTACAAAA 
TCCAAGGCAG AACCATATGT TTTGGTGGTG TTTTATGGGA ACAAGGTCTA CAATGTGAAA 
ATCCGTTTCC TCGAGAGCAA TCAACAGTTT GCCCTGGGCA CAGGACTACG AGGAAATGAG 
ATGTTTGATT CTGTGGAAGA CATCATTGAA CACTACACAT ATTTTCCCAT TCTGCTAATA 
GATGGGAAAG ACAAGGCTGC ACGCAGGAAA CAGTGCTACC TCACCCAGCC ACTGCCTCTC 
GCCAGGCTCC TTCTCACTCA GTACTCCAGC CAGGCACTTC ATGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Clnk ( XM_006503977.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006504040.1 Mus musculus cytokine-dependent hematopoietic cell linker (Clnk), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu35104
Clone ID Related Accession (Same CDS sequence)	XM_006503977.3 , XM_006503977.4
Accession Version	XM_006503977.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 708bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	cytokine-dependent hematopoietic cell linker isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006503977.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATGC AGAGGAGTGC AAAAGACTTC AGTAGGGTCC TTGGAGCAGA AGAAGAATCT 
CACCACCAGA CAAAGCCAGA ATCTTCTTGC CCATCATCAA ACCAAAACAC ACAGAAGAGT 
CCACCTGCCA TTGCCAGCTC TTCCTACATG CCAGGAAAGC ACAGTATACA AGCCAGAGAC 
CATACAGGTA GCATGCAGCA CTGTCCTGCT CAGAGATGCC AAGCTGCAGC CAGCCACAGC 
CCTCGAATGC TGCCCTATGA AAACACAAAC TCGGAGAAAC CTGACCCCAC AAAGCCTGAT 
GAGAAGGATG TCTGGCAGAA TGAATGGTAC ATTGGAGAAT ACAGTCGCCA GGCAGTGGAA 
GATGTGTTAA TGAAAGAGAA CAAGGATGGT ACTTTTTTGG TCCGAGACTG CTCTACAAAA 
TCCAAGGCAG AACCATATGT TTTGGTGGTG TTTTATGGGA ACAAGGTCTA CAATGTGAAA 
ATCCGTTTCC TCGAGAGCAA TCAACAGTTT GCCCTGGGCA CAGGACTACG AGGAAATGAG 
ATGTTTGATT CTGTGGAAGA CATCATTGAA CACTACACAT ATTTTCCCAT TCTGCTAATA 
GATGGGAAAG ACAAGGCTGC ACGCAGGAAA CAGTGCTACC TCACCCAGCC ACTGCCTCTC 
GCCAGGCTCC TTCTCACTCA GTACTCCAGC CAGGCACTTC ATGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006504040.1
CDS	128..835
Translation	MKMQRSAKDFSRVLGAEEESHHQTKPESSCPSSNQNTQKSPPAIASSSYMPGKHSIQARDHTGSMQHCPAQRCQAAASHSPRMLPYENTNSEKPDPTKPDEKDVWQNEWYIGEYSRQAVEDVLMKENKDGTFLVRDCSTKSKAEPYVLVVFYGNKVYNVKIRFLESNQQFALGTGLRGNEMFDSVEDIIEHYTYFPILLIDGKDKAARRKQCYLTQPLPLARLLLTQYSSQALHE

Target ORF information:

RefSeq Version	XM_006503977.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus cytokine-dependent hematopoietic cell linker (Clnk), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006503977.4

ORF Insert Sequence:

ATGAAAATGC AGAGGAGTGC AAAAGACTTC AGTAGGGTCC TTGGAGCAGA AGAAGAATCT 
CACCACCAGA CAAAGCCAGA ATCTTCTTGC CCATCATCAA ACCAAAACAC ACAGAAGAGT 
CCACCTGCCA TTGCCAGCTC TTCCTACATG CCAGGAAAGC ACAGTATACA AGCCAGAGAC 
CATACAGGTA GCATGCAGCA CTGTCCTGCT CAGAGATGCC AAGCTGCAGC CAGCCACAGC 
CCTCGAATGC TGCCCTATGA AAACACAAAC TCGGAGAAAC CTGACCCCAC AAAGCCTGAT 
GAGAAGGATG TCTGGCAGAA TGAATGGTAC ATTGGAGAAT ACAGTCGCCA GGCAGTGGAA 
GATGTGTTAA TGAAAGAGAA CAAGGATGGT ACTTTTTTGG TCCGAGACTG CTCTACAAAA 
TCCAAGGCAG AACCATATGT TTTGGTGGTG TTTTATGGGA ACAAGGTCTA CAATGTGAAA 
ATCCGTTTCC TCGAGAGCAA TCAACAGTTT GCCCTGGGCA CAGGACTACG AGGAAATGAG 
ATGTTTGATT CTGTGGAAGA CATCATTGAA CACTACACAT ATTTTCCCAT TCTGCTAATA 
GATGGGAAAG ACAAGGCTGC ACGCAGGAAA CAGTGCTACC TCACCCAGCC ACTGCCTCTC 
GCCAGGCTCC TTCTCACTCA GTACTCCAGC CAGGCACTTC ATGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Clnk plays a role in TNF-alpha-induced cell death in murine fibrosarcoma cell line L929.
Biochemical and biophysical research communications463(3)275-9(2015 Jul)
Xu M,Cai C,Sun X,Chen W,Li Q,Zhou H

Distinct regulatory functions of SLP-76 and MIST in NK cell cytotoxicity and IFN-gamma production.
International immunology20(3)345-52(2008 Mar)
Hidano S,Sasanuma H,Ohshima K,Seino K,Kumar L,Hayashi K,Hikida M,Kurosaki T,Taniguchi M,Geha RS,Kitamura D,Goitsuka R

Dual function for the adaptor MIST in IFN-gamma production by NK and CD4+NKT cells regulated by the Src kinase Fgr.
Blood107(9)3647-55(2006 May)
Sasanuma H,Tatsuno A,Hidano S,Ohshima K,Matsuzaki Y,Hayashi K,Lowell CA,Kitamura D,Goitsuka R

Transcriptional regulation of SLP-76 family hematopoietic cell adaptor MIST/Clnk by STAT5.
Biochemical and biophysical research communications321(1)145-53(2004 Aug)
Sasanuma H,Tatsuno A,Tsuji K,Hidano S,Morita S,Kitamura T,Kubo M,Kitamura D,Goitsuka R

Immune functions in mice lacking Clnk, an SLP-76-related adaptor expressed in a subset of immune cells.
Molecular and cellular biology24(13)6067-75(2004 Jul)
Utting O,Sedgmen BJ,Watts TH,Shi X,Rottapel R,Iulianella A,Lohnes D,Veillette A

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Clnk cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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