Pdk4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pdk4
Entrez Gene ID	27273
Full Name	pyruvate dehydrogenase kinase, isoenzyme 4
Synonyms	AV005916
General protein information	Preferred Names pyruvate dehydrogenase kinase, isoenzyme 4 Names [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4, mitochondrial pyruvate dehydrogenase kinase 4
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6 A1\|6 2.06 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_013743.2	NP_038771.1	[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PDK4	NP_001233460.1
Mus musculus (house mouse)	Pdk4	NP_038771.1
Bos taurus (cattle)	PDK4	NP_001095353.1
Rattus norvegicus (Norway rat)	Pdk4	NP_446003.1
Gallus gallus (chicken)	PDK4	NP_001186838.1
	PDK4	XP_001093471.1
Danio rerio (zebrafish)	pdk4	NP_001122176.1
Homo sapiens (human)	PDK4	NP_002603.1
Canis lupus familiaris (dog)	PDK4	XP_005628582.1

Gene Ontology
Bibliography

Related articles in PubMed

SIRT6 deacetylase transcriptionally regulates glucose metabolism in heart.
Khan D, Sarikhani M, Dasgupta S, Maniyadath B, Pandit AS, Mishra S, Ahamed F, Dubey A, Fathma N, Atreya HS, Kolthur-Seetharam U, Sundaresan NR
Journal of cellular physiology(2018 Jan)

Pyruvate dehydrogenase kinase 4 mediates lipogenesis and contributes to the pathogenesis of nonalcoholic steatohepatitis.
Zhang M, Zhao Y, Li Z, Wang C
Biochemical and biophysical research communications495(1)582-586(2018 01)

Pyruvate dehydrogenase kinase 4 exhibits a novel role in the activation of mutant KRAS, regulating cell growth in lung and colorectal tumour cells.
Trinidad AG, Whalley N, Rowlinson R, Delpuech O, Dudley P, Rooney C, Critchlow SE
Oncogene36(44)6164-6176(2017 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In conclusion, our data indicated that PDK4 potentially contributes to the hepatic steatosis in NASH via regulating several signaling pathway and PDK4 may be a new therapeutic strategy against NAFLD.
Title: Pyruvate dehydrogenase kinase 4 mediates lipogenesis and contributes to the pathogenesis of nonalcoholic steatohepatitis.

Taken together these data support a model where PDHK4 regulates KRAS signalling and its tumorigenic properties and suggest that inhibition of PDHK4 could represent a novel therapeutic strategy to target KRAS mutant colorectal and lung cancers
Title: Pyruvate dehydrogenase kinase 4 exhibits a novel role in the activation of mutant KRAS, regulating cell growth in lung and colorectal tumour cells.

Pyruvate dehydrogenase kinase 4 is a direct transcriptional target of FoxO1 in the heart. FoxO1 directly regulates Pdk4 transcription in the heart, thereby controlling PDH activity and subsequent glucose oxidation rates.
Title: FoxO1 regulates myocardial glucose oxidation rates via transcriptional control of pyruvate dehydrogenase kinase 4 expression.

PDK4 mediates cisplatin-induced acute kidney injury.
Title: Pyruvate dehydrogenase kinase 4 deficiency attenuates cisplatin-induced acute kidney injury.

inhibition of PDK4 activity in Hepatocellular carcinoma cells increased cyclin E1, cyclin A2, and E2F1 proteins.
Title: Pyruvate Dehydrogenase Kinase 4 Deficiency Results in Expedited Cellular Proliferation through E2F1-Mediated Increase of Cyclins.

The following Pdk4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pdk4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu18196	NM_013743.2 Latest version!	Mus musculus pyruvate dehydrogenase kinase, isoenzyme 4 (Pdk4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pdk4 ( NM_013743.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_038771.1 Mus musculus pyruvate dehydrogenase kinase, isoenzyme 4 (Pdk4), mRNA.

Price & Availability↑

CloneID	OMu18196
Clone ID Related Accession (Same CDS sequence)	NM_013743.2
Accession Version	NM_013743.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1239bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-31
Organism	Mus musculus(house mouse)
Product	[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC023174.4. On Nov 17, 2006 this sequence version replaced NM_013743.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ001418.1, AK004543.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164135 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGAAGGCAG CCCGCTTCGT GATGCGCAGC GCCAGCTCGC TGAGCAGCGC CAGCCTGGTC 
CCCAGGGAGG TCGAGCTGTT CTCCCGCTAC AGCCCGTCCC CGCTGTCCAT GAAGCAGCTG 
CTGGACTTTG GTTCAGAAAA TGCCTGTGAA AGAACGTCCT TTGCTTTTCT GCGGCAAGAG 
CTGCCCGTCC GCCTGGCCAA TATCCTGAAG GAGATTGACA TCCTGCCTGA CCGCTTAGTG 
AACACTCCTT CGGTGCAGCT GGTGAAGAGC TGGTATATCC AGAGCCTGAT GGATTTGGTG 
GAGTTCCATG AGAAGAGCCC AGAAGACCAG AAAGCCCTGT CAGAGTTTGT AGACACGCTG 
GTCAAAGTTC GAAACAGACA TCATAATGTG GTCCCTACAA TGGCTCAAGG CATCCTGGAG 
TATAAAGACA CCTGCACAGT GGACCCCGTT ACCAATCAAA ATCTTCAGTA TTTTTTAGAC 
CGGTTTTACA TGAACCGCAT TTCTACTCGG ATGCTCATGA ATCAGCACAT CCTCATATTC 
AGTGACTCAA AGACGGGAAA CCCAAGCCAC ATTGGAAGTA TCGACCCAAA CTGTGATGTG 
GTAGCAGTAG TCCAAGATGC CTTTGAGTGT GCAAAGATGC TCTGCGACCA GTATTATCTA 
ACATCGCCAG AATTAAACCT CACACAAGTC AATGGAAAAT TTCCAGGCCA ACCAATCCAC 
ATTGTGTACG TTCCTTCACA CCTTCACCAC ATGCTCTTCG AACTCTTCAA GAATGCCATG 
AGGGCCACGG TCGAGCATCA AGAAAACCGT CCTTCCTTGA CCCCAGTAGA GGCCACTGTC 
GTCTTGGGAA AAGAAGACCT TACAATCAAG ATTTCTGACC GAGGAGGCGG TGTTCCTCTG 
AGGATTACTG ACCGCCTCTT TAGTTACACG TACTCCACTG CTCCAACACC TGTGATGGAC 
AATTCCCGGA ATGCCCCTTT GGCTGGTTTT GGTTATGGCT TGCCAATTTC TCGTCTCTAC 
GCCAAGTATT TTCAAGGAGA TCTGAATCTC TACTCTATGT CAGGTTATGG GACAGACGCT 
ATCATCTACT TAAAGGCTTT ATCTTCTGAG TCTGTAGAAA AGCTCCCAGT CTTTAACAAG 
TCAGCCTTCA AACATTATCA GATGAGCTCC GAAGCTGATG ACTGGTGTAT CCCAAGCAGG 
GAACCGAAGA ACCTGGCGAA GGAGAAGCTG GCAGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038771.1
CDS	117..1355
Translation	MKAARFVMRSASSLSSASLVPREVELFSRYSPSPLSMKQLLDFGSENACERTSFAFLRQELPVRLANILKEIDILPDRLVNTPSVQLVKSWYIQSLMDLVEFHEKSPEDQKALSEFVDTLVKVRNRHHNVVPTMAQGILEYKDTCTVDPVTNQNLQYFLDRFYMNRISTRMLMNQHILIFSDSKTGNPSHIGSIDPNCDVVAVVQDAFECAKMLCDQYYLTSPELNLTQVNGKFPGQPIHIVYVPSHLHHMLFELFKNAMRATVEHQENRPSLTPVEATVVLGKEDLTIKISDRGGGVPLRITDRLFSYTYSTAPTPVMDNSRNAPLAGFGYGLPISRLYAKYFQGDLNLYSMSGYGTDAIIYLKALSSESVEKLPVFNKSAFKHYQMSSEADDWCIPSREPKNLAKEKLAV

Target ORF information:

RefSeq Version	NM_013743.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus pyruvate dehydrogenase kinase, isoenzyme 4 (Pdk4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013743.2

ORF Insert Sequence:

ATGAAGGCAG CCCGCTTCGT GATGCGCAGC GCCAGCTCGC TGAGCAGCGC CAGCCTGGTC 
CCCAGGGAGG TCGAGCTGTT CTCCCGCTAC AGCCCGTCCC CGCTGTCCAT GAAGCAGCTG 
CTGGACTTTG GTTCAGAAAA TGCCTGTGAA AGAACGTCCT TTGCTTTTCT GCGGCAAGAG 
CTGCCCGTCC GCCTGGCCAA TATCCTGAAG GAGATTGACA TCCTGCCTGA CCGCTTAGTG 
AACACTCCTT CGGTGCAGCT GGTGAAGAGC TGGTATATCC AGAGCCTGAT GGATTTGGTG 
GAGTTCCATG AGAAGAGCCC AGAAGACCAG AAAGCCCTGT CAGAGTTTGT AGACACGCTG 
GTCAAAGTTC GAAACAGACA TCATAATGTG GTCCCTACAA TGGCTCAAGG CATCCTGGAG 
TATAAAGACA CCTGCACAGT GGACCCCGTT ACCAATCAAA ATCTTCAGTA TTTTTTAGAC 
CGGTTTTACA TGAACCGCAT TTCTACTCGG ATGCTCATGA ATCAGCACAT CCTCATATTC 
AGTGACTCAA AGACGGGAAA CCCAAGCCAC ATTGGAAGTA TCGACCCAAA CTGTGATGTG 
GTAGCAGTAG TCCAAGATGC CTTTGAGTGT GCAAAGATGC TCTGCGACCA GTATTATCTA 
ACATCGCCAG AATTAAACCT CACACAAGTC AATGGAAAAT TTCCAGGCCA ACCAATCCAC 
ATTGTGTACG TTCCTTCACA CCTTCACCAC ATGCTCTTCG AACTCTTCAA GAATGCCATG 
AGGGCCACGG TCGAGCATCA AGAAAACCGT CCTTCCTTGA CCCCAGTAGA GGCCACTGTC 
GTCTTGGGAA AAGAAGACCT TACAATCAAG ATTTCTGACC GAGGAGGCGG TGTTCCTCTG 
AGGATTACTG ACCGCCTCTT TAGTTACACG TACTCCACTG CTCCAACACC TGTGATGGAC 
AATTCCCGGA ATGCCCCTTT GGCTGGTTTT GGTTATGGCT TGCCAATTTC TCGTCTCTAC 
GCCAAGTATT TTCAAGGAGA TCTGAATCTC TACTCTATGT CAGGTTATGG GACAGACGCT 
ATCATCTACT TAAAGGCTTT ATCTTCTGAG TCTGTAGAAA AGCTCCCAGT CTTTAACAAG 
TCAGCCTTCA AACATTATCA GATGAGCTCC GAAGCTGATG ACTGGTGTAT CCCAAGCAGG 
GAACCGAAGA ACCTGGCGAA GGAGAAGCTG GCAGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

SIRT6 deacetylase transcriptionally regulates glucose metabolism in heart.
Journal of cellular physiology(2018 Jan)
Khan D,Sarikhani M,Dasgupta S,Maniyadath B,Pandit AS,Mishra S,Ahamed F,Dubey A,Fathma N,Atreya HS,Kolthur-Seetharam U,Sundaresan NR

Pyruvate dehydrogenase kinase 4 mediates lipogenesis and contributes to the pathogenesis of nonalcoholic steatohepatitis.
Biochemical and biophysical research communications495(1)582-586(2018 01)
Zhang M,Zhao Y,Li Z,Wang C

Pyruvate dehydrogenase kinase 4 exhibits a novel role in the activation of mutant KRAS, regulating cell growth in lung and colorectal tumour cells.
Oncogene36(44)6164-6176(2017 Nov)
Trinidad AG,Whalley N,Rowlinson R,Delpuech O,Dudley P,Rooney C,Critchlow SE

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Pdk4 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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