Cars cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cars
Entrez Gene ID	27267
Full Name	cysteinyl-tRNA synthetase
Synonyms	CA3
General protein information	Preferred Names cysteinyl-tRNA synthetase Names cysteine--tRNA ligase, cytoplasmic cysRS cysteinyl-tRNA synthetase, cytoplasmic
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7 F5\|7 88.25 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_011242001.1	XP_011240303.1	cysteine--tRNA ligase, cytoplasmic isoform X3
XM_011242001.2	XP_011240303.1	cysteine--tRNA ligase, cytoplasmic isoform X3
XM_030242631.1	XP_030098491.1	cysteine--tRNA ligase, cytoplasmic isoform X3
XM_011242000.1	XP_011240302.1	cysteine--tRNA ligase, cytoplasmic isoform X3
XM_006508627.2	XP_006508690.1	cysteine--tRNA ligase, cytoplasmic isoform X1
NM_001252593.1	NP_001239522.1	cysteine--tRNA ligase, cytoplasmic isoform 2
XM_017322295.1	XP_017177784.1	cysteine--tRNA ligase, cytoplasmic isoform X2
NM_013742.5	NP_038770.3	cysteine--tRNA ligase, cytoplasmic isoform 1

Pathways from BioSystems

Homologies

	CARS	XP_001096302.1
Oryza sativa (rice)	Os09g0556500	NP_001063899.1
Homo sapiens (human)	CARS	NP_001014437.1
Rattus norvegicus (Norway rat)	Cars	NP_001099789.1
Drosophila melanogaster (fruit fly)	Aats-cys	NP_611087.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012283	XP_320253.2
Arabidopsis thaliana (thale cress)	SYCO ARATH	NP_565717.2
Mus musculus (house mouse)	Cars	NP_038770.3
Gallus gallus (chicken)	CARS	NP_001012601.1
Caenorhabditis elegans (roundworm)	cars-1	NP_491014.1
Saccharomyces cerevisiae (baker's yeast)	YNL247W	NP_014152.1
Kluyveromyces lactis	KLLA0C03025g	XP_452332.1
Pan troglodytes (chimpanzee)	CARS	XP_003312912.1
Canis lupus familiaris (dog)	CARS	XP_851224.1
Bos taurus (cattle)	CARS	NP_001096719.1
Xenopus tropicalis (tropical clawed frog)	cars	NP_001011365.1
Danio rerio (zebrafish)	cars	NP_001112372.1
Schizosaccharomyces pombe (fission yeast)	SPAC29E6.06c	NP_594564.1

Gene Ontology
Bibliography

Related articles in PubMed

A Dynamic Unfolded Protein Response Contributes to the Control of Cortical Neurogenesis.
Laguesse S, Creppe C, Nedialkova DD, Pr?vot PP, Borgs L, Huysseune S, Franco B, Duysens G, Krusy N, Lee G, Thelen N, Thiry M, Close P, Chariot A, Malgrange B, Leidel SA, Godin JD, Nguyen L
Developmental cell35(5)553-67(2015 Dec)

Kcnq1ot1 noncoding RNA mediates transcriptional gene silencing by interacting with Dnmt1.
Mohammad F, Mondal T, Guseva N, Pandey GK, Kanduri C
Development (Cambridge, England)137(15)2493-9(2010 Aug)

A germline-specific class of small RNAs binds mammalian Piwi proteins.
Girard A, Sachidanandam R, Hannon GJ, Carmell MA
Nature442(7099)199-202(2006 Jul)

Identification of a new murine tumor necrosis factor receptor locus that contains two novel murine receptors for tumor necrosis factor-related apoptosis-inducing ligand (TRAIL).
Schneider P, Olson D, Tardivel A, Browning B, Lugovskoy A, Gong D, Dobles M, Hertig S, Hofmann K, Van Vlijmen H, Hsu YM, Burkly LC, Tschopp J, Zheng TS
The Journal of biological chemistry278(7)5444-54(2003 Feb)

Gene density and transcription influence the localization of chromatin outside of chromosome territories detectable by FISH.
Mahy NL, Perry PE, Bickmore WA
The Journal of cell biology159(5)753-63(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Cars gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cars cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu69278	XM_011242000.1 Latest version!	Mus musculus cysteinyl-tRNA synthetase (Cars), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OMu05317	NM_001252593.1 Latest version!	Mus musculus cysteinyl-tRNA synthetase (Cars), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu40273	XM_006508627.2 Latest version!	Mus musculus cysteinyl-tRNA synthetase (Cars), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu09873	NM_013742.5 Latest version!	Mus musculus cysteinyl-tRNA synthetase (Cars), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
OMu69278	XM_011242001.2 Latest version!	Mus musculus cysteinyl-tRNA synthetase (Cars), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu69278	XM_030242631.1 Latest version!	Mus musculus cysteinyl-tRNA synthetase (Cars), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu82109	XM_017322295.1 Latest version!	Mus musculus cysteinyl-tRNA synthetase (Cars), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OMu69278	XM_011242001.1	Mus musculus cysteinyl-tRNA synthetase (Cars), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cars ( XM_011242001.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011240303.1 Mus musculus cysteinyl-tRNA synthetase (Cars), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu69278
Clone ID Related Accession (Same CDS sequence)	XM_011242001.1 , XM_011242001.2 , XM_030242631.1 , XM_011242000.1
Accession Version	XM_011242001.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	cysteine--tRNA ligase, cytoplasmic isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACATCA CAGACATTGA TGATAAGATC ATCAGGCGGG CACGGCAGAA CTACCTGTTT 
GAGCAGTATC GGGAGCAGAA ACCCCCAGCC ACCCAGCTCC TGAAGGATGT CCGTGATGCC 
ATGAAGCCAT TTTCAGTCAA GTTAAGTGAG ACAACAGATC CCGACAAGAG GCAGATGCTG 
GAGCGGATCC AGAACTCTGT GAAACTTGCC ACAGAACCAC TGGAACAGGC TGTGCGATCC 
AGCCTCTCTG GAGAGGAAGT AGACAGCAAA GTACAGGTGT TGCTGGAGGA AGCAAAGGAC 
TTGCTCTCTG ACTGGCTGGA TTCCACAGGT GGCAGTGAGG TGACTGACAA CTCGATTTTC 
TCCAAACTGC CCAAGTTCTG GGAAGAAGAG TTCCACAAAG ACATGGAAGC CCTGAATGTT 
CTCCCTCCTG ATGTCCTAAC CCGTGTCAGT GAATATGTGC CAGAAATTGT GAACTTCGTC 
CAGAAGATTG TGGACAATGG TTATGGTTAT GCTTCAAATG GATCTGTTTA CTTCGACACA 
GCCAAGTTTG CTGCTAGTGA AAAGCACTCC TATGGGAAGC TGGTGCCTGA GGCCGTTGGG 
GATCAGAAGG CACTTCAGGA AGGGGAAGGT GATCTGAGCA TCTCTGCTGA CCGCCTTAGT 
GAGAAACGCT CTCCCAATGA CTTTGCCTTG TGGAAGGCCT CCAAGCCAGG CGAGCCATCC 
TGGCCTTGCC CCTGGGGAAA GGGTCGTCCC GGATGGCACA TAGAGTGTTC TGCTATGGCA 
GGCACGCTCC TGGGAGCCTC AATGGACATT CATGGTGGAG GGTTTGACCT CCGCTTCCCC 
CACCATGACA ATGAGCTGGC ACAGTCGGAG GCCTACTTTG AAAATGACTG CTGGGTCAGG 
TACTTCTTGC ACACGGGCCA CTTGACGATA GCAGGCTGCA AGATGTCCAA GTCACTGAAA 
AACTTTATCA CCATTAAAGA TGCCTTGAAG AAGCACTCAG CACGGCAGCT GCGACTGGCA 
TTCCTCATGC ACTCATGGAA AGACACACTG GACTATTCCA GCAACACTAT GGAGTCTGCT 
CTTCAGTATG AAAAATTCAT GAACGAGTTT TTCTTAAATG TGAAAGACAT CCTCCGAGCC 
CCTGTAGACA TCACTGGCCA GTTTGAAAAG TGGGAAGCTG AAGAAGTGGA GCTAAATAAG 
AACTTCTATG GCAAGAAGAC CGCAGTTCAC GAAGCCTTGT GTGATAACAT TGACACCCGG 
ACTGTCATGG AAGAGATGCG GGCTTTGGTC AGTCAGTGCA ACCTCTACAT GGCAGCCAGG 
AAGGCTGAAC GGAGGAGGCC CAACCGGGCT CTGCTGGAGA ACATTGCCAT GTACCTCACC 
CACATGCTGA AGATCTTTGG GGCCATAGAG GAGGAGAGCC CCCTGGGGTT CCCAGTTGGT 
GGGCCTGGAA CCAACCTGAA CCTCGAGTCA ACAGTCATGC CCTACCTTCA GGTGTTATCA 
GAATTCAGAG AGGGAGTACG AAAGATTGCC CGAGAGAAAA AAGTTCTTGA GGTTCTACAG 
CTCAGTGATG CCCTCCGGGA TGACATCCTG CCTGAGCTTG GGGTCCGGTT TGAAGACCAT 
GAAGGGCTGC CAACAGTGGT GAAGTTGGTG GACAGAGACA CCTTACTGAA AGAGAAGGAA 
GGAAAGAAAA GGGCTGAAGA AGAGAAGAGG AGGAAGAAAG AGGAGGCGGC CAGAAAGAAA 
CAGGAGCAAG AAGCAGCAAA GCTGGCCAAG ATGAAGATAC CACCCAGTGA GATGTTCCTG 
TCAGAAGTCA ACAAGTATTC TAAATTTGAT GAAAATGGTC TGCCTACCCA CGACACCGAA 
GGCAAAGAGC TGAGCAAGGG CCAGGCCAAG AAGCTGAAGA AGCTCTTTGA AGCCCAGGAG 
AAACTGTACA AGGAGTATCT GCAAATGTTA CAGAATGGCA GCCTCCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011240303.1
CDS	233..2203
Translation	MNITDIDDKIIRRARQNYLFEQYREQKPPATQLLKDVRDAMKPFSVKLSETTDPDKRQMLERIQNSVKLATEPLEQAVRSSLSGEEVDSKVQVLLEEAKDLLSDWLDSTGGSEVTDNSIFSKLPKFWEEEFHKDMEALNVLPPDVLTRVSEYVPEIVNFVQKIVDNGYGYASNGSVYFDTAKFAASEKHSYGKLVPEAVGDQKALQEGEGDLSISADRLSEKRSPNDFALWKASKPGEPSWPCPWGKGRPGWHIECSAMAGTLLGASMDIHGGGFDLRFPHHDNELAQSEAYFENDCWVRYFLHTGHLTIAGCKMSKSLKNFITIKDALKKHSARQLRLAFLMHSWKDTLDYSSNTMESALQYEKFMNEFFLNVKDILRAPVDITGQFEKWEAEEVELNKNFYGKKTAVHEALCDNIDTRTVMEEMRALVSQCNLYMAARKAERRRPNRALLENIAMYLTHMLKIFGAIEEESPLGFPVGGPGTNLNLESTVMPYLQVLSEFREGVRKIAREKKVLEVLQLSDALRDDILPELGVRFEDHEGLPTVVKLVDRDTLLKEKEGKKRAEEEKRRKKEEAARKKQEQEAAKLAKMKIPPSEMFLSEVNKYSKFDENGLPTHDTEGKELSKGQAKKLKKLFEAQEKLYKEYLQMLQNGSLQ

Target ORF information:

RefSeq Version	XM_011242001.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus cysteinyl-tRNA synthetase (Cars), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011242001.1

ORF Insert Sequence:

ATGAACATCA CAGACATTGA TGATAAGATC ATCAGGCGGG CACGGCAGAA CTACCTGTTT 
GAGCAGTATC GGGAGCAGAA ACCCCCAGCC ACCCAGCTCC TGAAGGATGT CCGTGATGCC 
ATGAAGCCAT TTTCAGTCAA GTTAAGTGAG ACAACAGATC CCGACAAGAG GCAGATGCTG 
GAGCGGATCC AGAACTCTGT GAAACTTGCC ACAGAACCAC TGGAACAGGC TGTGCGATCC 
AGCCTCTCTG GAGAGGAAGT AGACAGCAAA GTACAGGTGT TGCTGGAGGA AGCAAAGGAC 
TTGCTCTCTG ACTGGCTGGA TTCCACAGGT GGCAGTGAGG TGACTGACAA CTCGATTTTC 
TCCAAACTGC CCAAGTTCTG GGAAGAAGAG TTCCACAAAG ACATGGAAGC CCTGAATGTT 
CTCCCTCCTG ATGTCCTAAC CCGTGTCAGT GAATATGTGC CAGAAATTGT GAACTTCGTC 
CAGAAGATTG TGGACAATGG TTATGGTTAT GCTTCAAATG GATCTGTTTA CTTCGACACA 
GCCAAGTTTG CTGCTAGTGA AAAGCACTCC TATGGGAAGC TGGTGCCTGA GGCCGTTGGG 
GATCAGAAGG CACTTCAGGA AGGGGAAGGT GATCTGAGCA TCTCTGCTGA CCGCCTTAGT 
GAGAAACGCT CTCCCAATGA CTTTGCCTTG TGGAAGGCCT CCAAGCCAGG CGAGCCATCC 
TGGCCTTGCC CCTGGGGAAA GGGTCGTCCC GGATGGCACA TAGAGTGTTC TGCTATGGCA 
GGCACGCTCC TGGGAGCCTC AATGGACATT CATGGTGGAG GGTTTGACCT CCGCTTCCCC 
CACCATGACA ATGAGCTGGC ACAGTCGGAG GCCTACTTTG AAAATGACTG CTGGGTCAGG 
TACTTCTTGC ACACGGGCCA CTTGACGATA GCAGGCTGCA AGATGTCCAA GTCACTGAAA 
AACTTTATCA CCATTAAAGA TGCCTTGAAG AAGCACTCAG CACGGCAGCT GCGACTGGCA 
TTCCTCATGC ACTCATGGAA AGACACACTG GACTATTCCA GCAACACTAT GGAGTCTGCT 
CTTCAGTATG AAAAATTCAT GAACGAGTTT TTCTTAAATG TGAAAGACAT CCTCCGAGCC 
CCTGTAGACA TCACTGGCCA GTTTGAAAAG TGGGAAGCTG AAGAAGTGGA GCTAAATAAG 
AACTTCTATG GCAAGAAGAC CGCAGTTCAC GAAGCCTTGT GTGATAACAT TGACACCCGG 
ACTGTCATGG AAGAGATGCG GGCTTTGGTC AGTCAGTGCA ACCTCTACAT GGCAGCCAGG 
AAGGCTGAAC GGAGGAGGCC CAACCGGGCT CTGCTGGAGA ACATTGCCAT GTACCTCACC 
CACATGCTGA AGATCTTTGG GGCCATAGAG GAGGAGAGCC CCCTGGGGTT CCCAGTTGGT 
GGGCCTGGAA CCAACCTGAA CCTCGAGTCA ACAGTCATGC CCTACCTTCA GGTGTTATCA 
GAATTCAGAG AGGGAGTACG AAAGATTGCC CGAGAGAAAA AAGTTCTTGA GGTTCTACAG 
CTCAGTGATG CCCTCCGGGA TGACATCCTG CCTGAGCTTG GGGTCCGGTT TGAAGACCAT 
GAAGGGCTGC CAACAGTGGT GAAGTTGGTG GACAGAGACA CCTTACTGAA AGAGAAGGAA 
GGAAAGAAAA GGGCTGAAGA AGAGAAGAGG AGGAAGAAAG AGGAGGCGGC CAGAAAGAAA 
CAGGAGCAAG AAGCAGCAAA GCTGGCCAAG ATGAAGATAC CACCCAGTGA GATGTTCCTG 
TCAGAAGTCA ACAAGTATTC TAAATTTGAT GAAAATGGTC TGCCTACCCA CGACACCGAA 
GGCAAAGAGC TGAGCAAGGG CCAGGCCAAG AAGCTGAAGA AGCTCTTTGA AGCCCAGGAG 
AAACTGTACA AGGAGTATCT GCAAATGTTA CAGAATGGCA GCCTCCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cars ( XM_011242000.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011240302.1 Mus musculus cysteinyl-tRNA synthetase (Cars), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu69278
Clone ID Related Accession (Same CDS sequence)	XM_011242001.1 , XM_011242001.2 , XM_030242631.1 , XM_011242000.1
Accession Version	XM_011242000.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	cysteine--tRNA ligase, cytoplasmic isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACATCA CAGACATTGA TGATAAGATC ATCAGGCGGG CACGGCAGAA CTACCTGTTT 
GAGCAGTATC GGGAGCAGAA ACCCCCAGCC ACCCAGCTCC TGAAGGATGT CCGTGATGCC 
ATGAAGCCAT TTTCAGTCAA GTTAAGTGAG ACAACAGATC CCGACAAGAG GCAGATGCTG 
GAGCGGATCC AGAACTCTGT GAAACTTGCC ACAGAACCAC TGGAACAGGC TGTGCGATCC 
AGCCTCTCTG GAGAGGAAGT AGACAGCAAA GTACAGGTGT TGCTGGAGGA AGCAAAGGAC 
TTGCTCTCTG ACTGGCTGGA TTCCACAGGT GGCAGTGAGG TGACTGACAA CTCGATTTTC 
TCCAAACTGC CCAAGTTCTG GGAAGAAGAG TTCCACAAAG ACATGGAAGC CCTGAATGTT 
CTCCCTCCTG ATGTCCTAAC CCGTGTCAGT GAATATGTGC CAGAAATTGT GAACTTCGTC 
CAGAAGATTG TGGACAATGG TTATGGTTAT GCTTCAAATG GATCTGTTTA CTTCGACACA 
GCCAAGTTTG CTGCTAGTGA AAAGCACTCC TATGGGAAGC TGGTGCCTGA GGCCGTTGGG 
GATCAGAAGG CACTTCAGGA AGGGGAAGGT GATCTGAGCA TCTCTGCTGA CCGCCTTAGT 
GAGAAACGCT CTCCCAATGA CTTTGCCTTG TGGAAGGCCT CCAAGCCAGG CGAGCCATCC 
TGGCCTTGCC CCTGGGGAAA GGGTCGTCCC GGATGGCACA TAGAGTGTTC TGCTATGGCA 
GGCACGCTCC TGGGAGCCTC AATGGACATT CATGGTGGAG GGTTTGACCT CCGCTTCCCC 
CACCATGACA ATGAGCTGGC ACAGTCGGAG GCCTACTTTG AAAATGACTG CTGGGTCAGG 
TACTTCTTGC ACACGGGCCA CTTGACGATA GCAGGCTGCA AGATGTCCAA GTCACTGAAA 
AACTTTATCA CCATTAAAGA TGCCTTGAAG AAGCACTCAG CACGGCAGCT GCGACTGGCA 
TTCCTCATGC ACTCATGGAA AGACACACTG GACTATTCCA GCAACACTAT GGAGTCTGCT 
CTTCAGTATG AAAAATTCAT GAACGAGTTT TTCTTAAATG TGAAAGACAT CCTCCGAGCC 
CCTGTAGACA TCACTGGCCA GTTTGAAAAG TGGGAAGCTG AAGAAGTGGA GCTAAATAAG 
AACTTCTATG GCAAGAAGAC CGCAGTTCAC GAAGCCTTGT GTGATAACAT TGACACCCGG 
ACTGTCATGG AAGAGATGCG GGCTTTGGTC AGTCAGTGCA ACCTCTACAT GGCAGCCAGG 
AAGGCTGAAC GGAGGAGGCC CAACCGGGCT CTGCTGGAGA ACATTGCCAT GTACCTCACC 
CACATGCTGA AGATCTTTGG GGCCATAGAG GAGGAGAGCC CCCTGGGGTT CCCAGTTGGT 
GGGCCTGGAA CCAACCTGAA CCTCGAGTCA ACAGTCATGC CCTACCTTCA GGTGTTATCA 
GAATTCAGAG AGGGAGTACG AAAGATTGCC CGAGAGAAAA AAGTTCTTGA GGTTCTACAG 
CTCAGTGATG CCCTCCGGGA TGACATCCTG CCTGAGCTTG GGGTCCGGTT TGAAGACCAT 
GAAGGGCTGC CAACAGTGGT GAAGTTGGTG GACAGAGACA CCTTACTGAA AGAGAAGGAA 
GGAAAGAAAA GGGCTGAAGA AGAGAAGAGG AGGAAGAAAG AGGAGGCGGC CAGAAAGAAA 
CAGGAGCAAG AAGCAGCAAA GCTGGCCAAG ATGAAGATAC CACCCAGTGA GATGTTCCTG 
TCAGAAGTCA ACAAGTATTC TAAATTTGAT GAAAATGGTC TGCCTACCCA CGACACCGAA 
GGCAAAGAGC TGAGCAAGGG CCAGGCCAAG AAGCTGAAGA AGCTCTTTGA AGCCCAGGAG 
AAACTGTACA AGGAGTATCT GCAAATGTTA CAGAATGGCA GCCTCCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011240302.1
CDS	281..2251
Translation	MNITDIDDKIIRRARQNYLFEQYREQKPPATQLLKDVRDAMKPFSVKLSETTDPDKRQMLERIQNSVKLATEPLEQAVRSSLSGEEVDSKVQVLLEEAKDLLSDWLDSTGGSEVTDNSIFSKLPKFWEEEFHKDMEALNVLPPDVLTRVSEYVPEIVNFVQKIVDNGYGYASNGSVYFDTAKFAASEKHSYGKLVPEAVGDQKALQEGEGDLSISADRLSEKRSPNDFALWKASKPGEPSWPCPWGKGRPGWHIECSAMAGTLLGASMDIHGGGFDLRFPHHDNELAQSEAYFENDCWVRYFLHTGHLTIAGCKMSKSLKNFITIKDALKKHSARQLRLAFLMHSWKDTLDYSSNTMESALQYEKFMNEFFLNVKDILRAPVDITGQFEKWEAEEVELNKNFYGKKTAVHEALCDNIDTRTVMEEMRALVSQCNLYMAARKAERRRPNRALLENIAMYLTHMLKIFGAIEEESPLGFPVGGPGTNLNLESTVMPYLQVLSEFREGVRKIAREKKVLEVLQLSDALRDDILPELGVRFEDHEGLPTVVKLVDRDTLLKEKEGKKRAEEEKRRKKEEAARKKQEQEAAKLAKMKIPPSEMFLSEVNKYSKFDENGLPTHDTEGKELSKGQAKKLKKLFEAQEKLYKEYLQMLQNGSLQ

Target ORF information:

RefSeq Version	XM_011242000.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus cysteinyl-tRNA synthetase (Cars), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011242000.1

ORF Insert Sequence:

ATGAACATCA CAGACATTGA TGATAAGATC ATCAGGCGGG CACGGCAGAA CTACCTGTTT 
GAGCAGTATC GGGAGCAGAA ACCCCCAGCC ACCCAGCTCC TGAAGGATGT CCGTGATGCC 
ATGAAGCCAT TTTCAGTCAA GTTAAGTGAG ACAACAGATC CCGACAAGAG GCAGATGCTG 
GAGCGGATCC AGAACTCTGT GAAACTTGCC ACAGAACCAC TGGAACAGGC TGTGCGATCC 
AGCCTCTCTG GAGAGGAAGT AGACAGCAAA GTACAGGTGT TGCTGGAGGA AGCAAAGGAC 
TTGCTCTCTG ACTGGCTGGA TTCCACAGGT GGCAGTGAGG TGACTGACAA CTCGATTTTC 
TCCAAACTGC CCAAGTTCTG GGAAGAAGAG TTCCACAAAG ACATGGAAGC CCTGAATGTT 
CTCCCTCCTG ATGTCCTAAC CCGTGTCAGT GAATATGTGC CAGAAATTGT GAACTTCGTC 
CAGAAGATTG TGGACAATGG TTATGGTTAT GCTTCAAATG GATCTGTTTA CTTCGACACA 
GCCAAGTTTG CTGCTAGTGA AAAGCACTCC TATGGGAAGC TGGTGCCTGA GGCCGTTGGG 
GATCAGAAGG CACTTCAGGA AGGGGAAGGT GATCTGAGCA TCTCTGCTGA CCGCCTTAGT 
GAGAAACGCT CTCCCAATGA CTTTGCCTTG TGGAAGGCCT CCAAGCCAGG CGAGCCATCC 
TGGCCTTGCC CCTGGGGAAA GGGTCGTCCC GGATGGCACA TAGAGTGTTC TGCTATGGCA 
GGCACGCTCC TGGGAGCCTC AATGGACATT CATGGTGGAG GGTTTGACCT CCGCTTCCCC 
CACCATGACA ATGAGCTGGC ACAGTCGGAG GCCTACTTTG AAAATGACTG CTGGGTCAGG 
TACTTCTTGC ACACGGGCCA CTTGACGATA GCAGGCTGCA AGATGTCCAA GTCACTGAAA 
AACTTTATCA CCATTAAAGA TGCCTTGAAG AAGCACTCAG CACGGCAGCT GCGACTGGCA 
TTCCTCATGC ACTCATGGAA AGACACACTG GACTATTCCA GCAACACTAT GGAGTCTGCT 
CTTCAGTATG AAAAATTCAT GAACGAGTTT TTCTTAAATG TGAAAGACAT CCTCCGAGCC 
CCTGTAGACA TCACTGGCCA GTTTGAAAAG TGGGAAGCTG AAGAAGTGGA GCTAAATAAG 
AACTTCTATG GCAAGAAGAC CGCAGTTCAC GAAGCCTTGT GTGATAACAT TGACACCCGG 
ACTGTCATGG AAGAGATGCG GGCTTTGGTC AGTCAGTGCA ACCTCTACAT GGCAGCCAGG 
AAGGCTGAAC GGAGGAGGCC CAACCGGGCT CTGCTGGAGA ACATTGCCAT GTACCTCACC 
CACATGCTGA AGATCTTTGG GGCCATAGAG GAGGAGAGCC CCCTGGGGTT CCCAGTTGGT 
GGGCCTGGAA CCAACCTGAA CCTCGAGTCA ACAGTCATGC CCTACCTTCA GGTGTTATCA 
GAATTCAGAG AGGGAGTACG AAAGATTGCC CGAGAGAAAA AAGTTCTTGA GGTTCTACAG 
CTCAGTGATG CCCTCCGGGA TGACATCCTG CCTGAGCTTG GGGTCCGGTT TGAAGACCAT 
GAAGGGCTGC CAACAGTGGT GAAGTTGGTG GACAGAGACA CCTTACTGAA AGAGAAGGAA 
GGAAAGAAAA GGGCTGAAGA AGAGAAGAGG AGGAAGAAAG AGGAGGCGGC CAGAAAGAAA 
CAGGAGCAAG AAGCAGCAAA GCTGGCCAAG ATGAAGATAC CACCCAGTGA GATGTTCCTG 
TCAGAAGTCA ACAAGTATTC TAAATTTGAT GAAAATGGTC TGCCTACCCA CGACACCGAA 
GGCAAAGAGC TGAGCAAGGG CCAGGCCAAG AAGCTGAAGA AGCTCTTTGA AGCCCAGGAG 
AAACTGTACA AGGAGTATCT GCAAATGTTA CAGAATGGCA GCCTCCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cars ( NM_001252593.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001239522.1 Mus musculus cysteinyl-tRNA synthetase (Cars), transcript variant 2, mRNA.

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CloneID	OMu05317
Clone ID Related Accession (Same CDS sequence)	NM_001252593.1
Accession Version	NM_001252593.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2247bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Mus musculus(house mouse)
Product	cysteine--tRNA ligase, cytoplasmic isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY253195.1, BY021374.1, AK152866.1 and BC030473.1. Transcript Variant: This variant (2) lacks an alternate, in-frame, exon in the 5' coding region, compared to variant 1. The resulting protein (isoform 2) is shorter when it is compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK152866.1, AK037227.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164138, SAMN01164139 [ECO:0000348] ##Evidence-Data-END##

ATGGCAGGTT CCTCCGCGGA GCAGGGTAAA GGCCGGCGAG TGCAGCCTCA GTGGTCCCCC 
CCAGCAGGGA CTGAGCCCTG CAGGCTCCGC CTCTATAATA GCCTCACCCG GAACAAGGAT 
GTATTTATAC CTCAAGATGG GAAGAAGGTG ACGTGGTACT GTTGCGGGCC GACTGTCTAT 
GATGCGTCTC ACATGGGACA TGCCAGGTCC TACATCTCCT TCGATATCCT GAGGAGGGTG 
CTGAGGGATT ACTTCCAGTA TGATGTCTTT TACTGCATGA ACATCACAGA CATTGATGAT 
AAGATCATCA GGCGGGCACG GCAGAACTAC CTGTTTGAGC AGTATCGGGA GCAGAAACCC 
CCAGCCACCC AGCTCCTGAA GGATGTCCGT GATGCCATGA AGCCATTTTC AGTCAAGTTA 
AGTGAGACAA CAGATCCCGA CAAGAGGCAG ATGCTGGAGC GGATCCAGAA CTCTGTGAAA 
CTTGCCACAG AACCACTGGA ACAGGCTGTG CGATCCAGCC TCTCTGGAGA GGAAGTAGAC 
AGCAAAGTAC AGGTGTTGCT GGAGGAAGCA AAGGACTTGC TCTCTGACTG GCTGGATTCC 
ACAGGTGGCA GTGAGGTGAC TGACAACTCG ATTTTCTCCA AACTGCCCAA GTTCTGGGAA 
GAAGAGTTCC ACAAAGACAT GGAAGCCCTG AATGTTCTCC CTCCTGATGT CCTAACCCGT 
GTCAGTGAAT ATGTGCCAGA AATTGTGAAC TTCGTCCAGA AGATTGTGGA CAATGGTTAT 
GGTTATGCTT CAAATGGATC TGTTTACTTC GACACAGCCA AGTTTGCTGC TAGTGAAAAG 
CACTCCTATG GGAAGCTGGT GCCTGAGGCC GTTGGGGATC AGAAGGCACT TCAGGAAGGG 
GAAGGTGATC TGAGCATCTC TGCTGACCGC CTTAGTGAGA AACGCTCTCC CAATGACTTT 
GCCTTGTGGA AGGCCTCCAA GCCAGGCGAG CCATCCTGGC CTTGCCCCTG GGGAAAGGGT 
CGTCCCGGAT GGCACATAGA GTGTTCTGCT ATGGCAGGCA CGCTCCTGGG AGCCTCAATG 
GACATTCATG GTGGAGGGTT TGACCTCCGC TTCCCCCACC ATGACAATGA GCTGGCACAG 
TCGGAGGCCT ACTTTGAAAA TGACTGCTGG GTCAGGTACT TCTTGCACAC GGGCCACTTG 
ACGATAGCAG GCTGCAAGAT GTCCAAGTCA CTGAAAAACT TTATCACCAT TAAAGATGCC 
TTGAAGAAGC ACTCAGCACG GCAGCTGCGA CTGGCATTCC TCATGCACTC ATGGAAAGAC 
ACACTGGACT ATTCCAGCAA CACTATGGAG TCTGCTCTTC AGTATGAAAA ATTCATGAAC 
GAGTTTTTCT TAAATGTGAA AGACATCCTC CGAGCCCCTG TAGACATCAC TGGCCAGTTT 
GAAAAGTGGG AAGCTGAAGA AGTGGAGCTA AATAAGAACT TCTATGGCAA GAAGACCGCA 
GTTCACGAAG CCTTGTGTGA TAACATTGAC ACCCGGACTG TCATGGAAGA GATGCGGGCT 
TTGGTCAGTC AGTGCAACCT CTACATGGCA GCCAGGAAGG CTGAACGGAG GAGGCCCAAC 
CGGGCTCTGC TGGAGAACAT TGCCATGTAC CTCACCCACA TGCTGAAGAT CTTTGGGGCC 
ATAGAGGAGG AGAGCCCCCT GGGGTTCCCA GTTGGTGGGC CTGGAACCAA CCTGAACCTC 
GAGTCAACAG TCATGCCCTA CCTTCAGGTG TTATCAGAAT TCAGAGAGGG AGTACGAAAG 
ATTGCCCGAG AGAAAAAAGT TCTTGAGGTT CTACAGCTCA GTGATGCCCT CCGGGATGAC 
ATCCTGCCTG AGCTTGGGGT CCGGTTTGAA GACCATGAAG GGCTGCCAAC AGTGGTGAAG 
TTGGTGGACA GAGACACCTT ACTGAAAGAG AAGGAAGGAA AGAAAAGGGC TGAAGAAGAG 
AAGAGGAGGA AGAAAGAGGA GGCGGCCAGA AAGAAACAGG AGCAAGAAGC AGCAAAGCTG 
GCCAAGATGA AGATACCACC CAGTGAGATG TTCCTGTCAG AAGTCAACAA GTATTCTAAA 
TTTGATGAAA ATGGTCTGCC TACCCACGAC ACCGAAGGCA AAGAGCTGAG CAAGGGCCAG 
GCCAAGAAGC TGAAGAAGCT CTTTGAAGCC CAGGAGAAAC TGTACAAGGA GTATCTGCAA 
ATGTTACAGA ATGGCAGCCT CCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001239522.1
CDS	85..2331
Translation	MAGSSAEQGKGRRVQPQWSPPAGTEPCRLRLYNSLTRNKDVFIPQDGKKVTWYCCGPTVYDASHMGHARSYISFDILRRVLRDYFQYDVFYCMNITDIDDKIIRRARQNYLFEQYREQKPPATQLLKDVRDAMKPFSVKLSETTDPDKRQMLERIQNSVKLATEPLEQAVRSSLSGEEVDSKVQVLLEEAKDLLSDWLDSTGGSEVTDNSIFSKLPKFWEEEFHKDMEALNVLPPDVLTRVSEYVPEIVNFVQKIVDNGYGYASNGSVYFDTAKFAASEKHSYGKLVPEAVGDQKALQEGEGDLSISADRLSEKRSPNDFALWKASKPGEPSWPCPWGKGRPGWHIECSAMAGTLLGASMDIHGGGFDLRFPHHDNELAQSEAYFENDCWVRYFLHTGHLTIAGCKMSKSLKNFITIKDALKKHSARQLRLAFLMHSWKDTLDYSSNTMESALQYEKFMNEFFLNVKDILRAPVDITGQFEKWEAEEVELNKNFYGKKTAVHEALCDNIDTRTVMEEMRALVSQCNLYMAARKAERRRPNRALLENIAMYLTHMLKIFGAIEEESPLGFPVGGPGTNLNLESTVMPYLQVLSEFREGVRKIAREKKVLEVLQLSDALRDDILPELGVRFEDHEGLPTVVKLVDRDTLLKEKEGKKRAEEEKRRKKEEAARKKQEQEAAKLAKMKIPPSEMFLSEVNKYSKFDENGLPTHDTEGKELSKGQAKKLKKLFEAQEKLYKEYLQMLQNGSLQ

Target ORF information:

RefSeq Version	NM_001252593.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus cysteinyl-tRNA synthetase (Cars), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001252593.1

ORF Insert Sequence:

ATGGCAGGTT CCTCCGCGGA GCAGGGTAAA GGCCGGCGAG TGCAGCCTCA GTGGTCCCCC 
CCAGCAGGGA CTGAGCCCTG CAGGCTCCGC CTCTATAATA GCCTCACCCG GAACAAGGAT 
GTATTTATAC CTCAAGATGG GAAGAAGGTG ACGTGGTACT GTTGCGGGCC GACTGTCTAT 
GATGCGTCTC ACATGGGACA TGCCAGGTCC TACATCTCCT TCGATATCCT GAGGAGGGTG 
CTGAGGGATT ACTTCCAGTA TGATGTCTTT TACTGCATGA ACATCACAGA CATTGATGAT 
AAGATCATCA GGCGGGCACG GCAGAACTAC CTGTTTGAGC AGTATCGGGA GCAGAAACCC 
CCAGCCACCC AGCTCCTGAA GGATGTCCGT GATGCCATGA AGCCATTTTC AGTCAAGTTA 
AGTGAGACAA CAGATCCCGA CAAGAGGCAG ATGCTGGAGC GGATCCAGAA CTCTGTGAAA 
CTTGCCACAG AACCACTGGA ACAGGCTGTG CGATCCAGCC TCTCTGGAGA GGAAGTAGAC 
AGCAAAGTAC AGGTGTTGCT GGAGGAAGCA AAGGACTTGC TCTCTGACTG GCTGGATTCC 
ACAGGTGGCA GTGAGGTGAC TGACAACTCG ATTTTCTCCA AACTGCCCAA GTTCTGGGAA 
GAAGAGTTCC ACAAAGACAT GGAAGCCCTG AATGTTCTCC CTCCTGATGT CCTAACCCGT 
GTCAGTGAAT ATGTGCCAGA AATTGTGAAC TTCGTCCAGA AGATTGTGGA CAATGGTTAT 
GGTTATGCTT CAAATGGATC TGTTTACTTC GACACAGCCA AGTTTGCTGC TAGTGAAAAG 
CACTCCTATG GGAAGCTGGT GCCTGAGGCC GTTGGGGATC AGAAGGCACT TCAGGAAGGG 
GAAGGTGATC TGAGCATCTC TGCTGACCGC CTTAGTGAGA AACGCTCTCC CAATGACTTT 
GCCTTGTGGA AGGCCTCCAA GCCAGGCGAG CCATCCTGGC CTTGCCCCTG GGGAAAGGGT 
CGTCCCGGAT GGCACATAGA GTGTTCTGCT ATGGCAGGCA CGCTCCTGGG AGCCTCAATG 
GACATTCATG GTGGAGGGTT TGACCTCCGC TTCCCCCACC ATGACAATGA GCTGGCACAG 
TCGGAGGCCT ACTTTGAAAA TGACTGCTGG GTCAGGTACT TCTTGCACAC GGGCCACTTG 
ACGATAGCAG GCTGCAAGAT GTCCAAGTCA CTGAAAAACT TTATCACCAT TAAAGATGCC 
TTGAAGAAGC ACTCAGCACG GCAGCTGCGA CTGGCATTCC TCATGCACTC ATGGAAAGAC 
ACACTGGACT ATTCCAGCAA CACTATGGAG TCTGCTCTTC AGTATGAAAA ATTCATGAAC 
GAGTTTTTCT TAAATGTGAA AGACATCCTC CGAGCCCCTG TAGACATCAC TGGCCAGTTT 
GAAAAGTGGG AAGCTGAAGA AGTGGAGCTA AATAAGAACT TCTATGGCAA GAAGACCGCA 
GTTCACGAAG CCTTGTGTGA TAACATTGAC ACCCGGACTG TCATGGAAGA GATGCGGGCT 
TTGGTCAGTC AGTGCAACCT CTACATGGCA GCCAGGAAGG CTGAACGGAG GAGGCCCAAC 
CGGGCTCTGC TGGAGAACAT TGCCATGTAC CTCACCCACA TGCTGAAGAT CTTTGGGGCC 
ATAGAGGAGG AGAGCCCCCT GGGGTTCCCA GTTGGTGGGC CTGGAACCAA CCTGAACCTC 
GAGTCAACAG TCATGCCCTA CCTTCAGGTG TTATCAGAAT TCAGAGAGGG AGTACGAAAG 
ATTGCCCGAG AGAAAAAAGT TCTTGAGGTT CTACAGCTCA GTGATGCCCT CCGGGATGAC 
ATCCTGCCTG AGCTTGGGGT CCGGTTTGAA GACCATGAAG GGCTGCCAAC AGTGGTGAAG 
TTGGTGGACA GAGACACCTT ACTGAAAGAG AAGGAAGGAA AGAAAAGGGC TGAAGAAGAG 
AAGAGGAGGA AGAAAGAGGA GGCGGCCAGA AAGAAACAGG AGCAAGAAGC AGCAAAGCTG 
GCCAAGATGA AGATACCACC CAGTGAGATG TTCCTGTCAG AAGTCAACAA GTATTCTAAA 
TTTGATGAAA ATGGTCTGCC TACCCACGAC ACCGAAGGCA AAGAGCTGAG CAAGGGCCAG 
GCCAAGAAGC TGAAGAAGCT CTTTGAAGCC CAGGAGAAAC TGTACAAGGA GTATCTGCAA 
ATGTTACAGA ATGGCAGCCT CCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cars ( XM_006508627.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508690.1 Mus musculus cysteinyl-tRNA synthetase (Cars), transcript variant X1, mRNA.

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CloneID	OMu40273
Clone ID Related Accession (Same CDS sequence)	XM_006508627.2
Accession Version	XM_006508627.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2436bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	cysteine--tRNA ligase, cytoplasmic isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006508627.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGTT CCTCCGCGGA GCAGGCTGCC GACTACAGGT CCATTCTGAG CATTAGTGAC 
GAGGCAGCCA GGGTGCAGGC CCTGGACCAA CACCTCAGCA CGCGTAGCTA TATCCAGGGG 
TACTCCCTAT CCCAGGCAGA TGTGGACGTG TTCAGGCAGC TCTCGGCCCC TCCCGCTGAC 
TCAAGGCTCT TCCACGTGGC TCGGTGGTTC AGGCACATAG AAGCGCTCCT GGGTGGCCCC 
CAAGGCAGAG ACGAGCCCTG CAGGCTTCAA GCAAGTAAAG GCCGGCGAGT GCAGCCTCAG 
TGGTCCCCCC CAGCAGGGAC TGAGCCCTGC AGGCTCCGCC TCTATAATAG CCTCACCCGG 
AACAAGGATG TATTTATACC TCAAGATGGG AAGAAGGTGA CGTGGTACTG TTGCGGGCCG 
ACTGTCTATG ATGCGTCTCA CATGGGACAT GCCAGGTCCT ACATCTCCTT CGATATCCTG 
AGGAGGGTGC TGAGGGATTA CTTCCAGTAT GATGTCTTTT ACTGCATGAA CATCACAGAC 
ATTGATGATA AGATCATCAG GCGGGCACGG CAGAACTACC TGTTTGAGCA GTATCGGGAG 
CAGAAACCCC CAGCCACCCA GCTCCTGAAG GATGTCCGTG ATGCCATGAA GCCATTTTCA 
GTCAAGTTAA GTGAGACAAC AGATCCCGAC AAGAGGCAGA TGCTGGAGCG GATCCAGAAC 
TCTGTGAAAC TTGCCACAGA ACCACTGGAA CAGGCTGTGC GATCCAGCCT CTCTGGAGAG 
GAAGTAGACA GCAAAGTACA GGTGTTGCTG GAGGAAGCAA AGGACTTGCT CTCTGACTGG 
CTGGATTCCA CAGGTGGCAG TGAGGTGACT GACAACTCGA TTTTCTCCAA ACTGCCCAAG 
TTCTGGGAAG AAGAGTTCCA CAAAGACATG GAAGCCCTGA ATGTTCTCCC TCCTGATGTC 
CTAACCCGTG TCAGTGAATA TGTGCCAGAA ATTGTGAACT TCGTCCAGAA GATTGTGGAC 
AATGGTTATG GTTATGCTTC AAATGGATCT GTTTACTTCG ACACAGCCAA GTTTGCTGCT 
AGTGAAAAGC ACTCCTATGG GAAGCTGGTG CCTGAGGCCG TTGGGGATCA GAAGGCACTT 
CAGGAAGGGG AAGGTGATCT GAGCATCTCT GCTGACCGCC TTAGTGAGAA ACGCTCTCCC 
AATGACTTTG CCTTGTGGAA GGCCTCCAAG CCAGGCGAGC CATCCTGGCC TTGCCCCTGG 
GGAAAGGGTC GTCCCGGATG GCACATAGAG TGTTCTGCTA TGGCAGGCAC GCTCCTGGGA 
GCCTCAATGG ACATTCATGG TGGAGGGTTT GACCTCCGCT TCCCCCACCA TGACAATGAG 
CTGGCACAGT CGGAGGCCTA CTTTGAAAAT GACTGCTGGG TCAGGTACTT CTTGCACACG 
GGCCACTTGA CGATAGCAGG CTGCAAGATG TCCAAGTCAC TGAAAAACTT TATCACCATT 
AAAGATGCCT TGAAGAAGCA CTCAGCACGG CAGCTGCGAC TGGCATTCCT CATGCACTCA 
TGGAAAGACA CACTGGACTA TTCCAGCAAC ACTATGGAGT CTGCTCTTCA GTATGAAAAA 
TTCATGAACG AGTTTTTCTT AAATGTGAAA GACATCCTCC GAGCCCCTGT AGACATCACT 
GGCCAGTTTG AAAAGTGGGA AGCTGAAGAA GTGGAGCTAA ATAAGAACTT CTATGGCAAG 
AAGACCGCAG TTCACGAAGC CTTGTGTGAT AACATTGACA CCCGGACTGT CATGGAAGAG 
ATGCGGGCTT TGGTCAGTCA GTGCAACCTC TACATGGCAG CCAGGAAGGC TGAACGGAGG 
AGGCCCAACC GGGCTCTGCT GGAGAACATT GCCATGTACC TCACCCACAT GCTGAAGATC 
TTTGGGGCCA TAGAGGAGGA GAGCCCCCTG GGGTTCCCAG TTGGTGGGCC TGGAACCAAC 
CTGAACCTCG AGTCAACAGT CATGCCCTAC CTTCAGGTGT TATCAGAATT CAGAGAGGGA 
GTACGAAAGA TTGCCCGAGA GAAAAAAGTT CTTGAGGTTC TACAGCTCAG TGATGCCCTC 
CGGGATGACA TCCTGCCTGA GCTTGGGGTC CGGTTTGAAG ACCATGAAGG GCTGCCAACA 
GTGGTGAAGT TGGTGGACAG AGACACCTTA CTGAAAGAGA AGGAAGGAAA GAAAAGGGCA 
GCAAAGCTGG CCAAGATGAA GATACCACCC AGTGAGATGT TCCTGTCAGA AGTCAACAAG 
TATTCTAAAT TTGATGAAAA TGGTCTGCCT ACCCACGACA CCGAAGGCAA AGAGCTGAGC 
AAGGGCCAGG CCAAGAAGCT GAAGAAGCTC TTTGAAGCCC AGGAGAAACT GTACAAGGAG 
TATCTGCAAA TGTTACAGAA TGGCAGCCTC CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508690.1
CDS	83..2518
Translation	MAGSSAEQAADYRSILSISDEAARVQALDQHLSTRSYIQGYSLSQADVDVFRQLSAPPADSRLFHVARWFRHIEALLGGPQGRDEPCRLQASKGRRVQPQWSPPAGTEPCRLRLYNSLTRNKDVFIPQDGKKVTWYCCGPTVYDASHMGHARSYISFDILRRVLRDYFQYDVFYCMNITDIDDKIIRRARQNYLFEQYREQKPPATQLLKDVRDAMKPFSVKLSETTDPDKRQMLERIQNSVKLATEPLEQAVRSSLSGEEVDSKVQVLLEEAKDLLSDWLDSTGGSEVTDNSIFSKLPKFWEEEFHKDMEALNVLPPDVLTRVSEYVPEIVNFVQKIVDNGYGYASNGSVYFDTAKFAASEKHSYGKLVPEAVGDQKALQEGEGDLSISADRLSEKRSPNDFALWKASKPGEPSWPCPWGKGRPGWHIECSAMAGTLLGASMDIHGGGFDLRFPHHDNELAQSEAYFENDCWVRYFLHTGHLTIAGCKMSKSLKNFITIKDALKKHSARQLRLAFLMHSWKDTLDYSSNTMESALQYEKFMNEFFLNVKDILRAPVDITGQFEKWEAEEVELNKNFYGKKTAVHEALCDNIDTRTVMEEMRALVSQCNLYMAARKAERRRPNRALLENIAMYLTHMLKIFGAIEEESPLGFPVGGPGTNLNLESTVMPYLQVLSEFREGVRKIAREKKVLEVLQLSDALRDDILPELGVRFEDHEGLPTVVKLVDRDTLLKEKEGKKRAAKLAKMKIPPSEMFLSEVNKYSKFDENGLPTHDTEGKELSKGQAKKLKKLFEAQEKLYKEYLQMLQNGSLQ

Target ORF information:

RefSeq Version	XM_006508627.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus cysteinyl-tRNA synthetase (Cars), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508627.2

ORF Insert Sequence:

ATGGCAGGTT CCTCCGCGGA GCAGGCTGCC GACTACAGGT CCATTCTGAG CATTAGTGAC 
GAGGCAGCCA GGGTGCAGGC CCTGGACCAA CACCTCAGCA CGCGTAGCTA TATCCAGGGG 
TACTCCCTAT CCCAGGCAGA TGTGGACGTG TTCAGGCAGC TCTCGGCCCC TCCCGCTGAC 
TCAAGGCTCT TCCACGTGGC TCGGTGGTTC AGGCACATAG AAGCGCTCCT GGGTGGCCCC 
CAAGGCAGAG ACGAGCCCTG CAGGCTTCAA GCAAGTAAAG GCCGGCGAGT GCAGCCTCAG 
TGGTCCCCCC CAGCAGGGAC TGAGCCCTGC AGGCTCCGCC TCTATAATAG CCTCACCCGG 
AACAAGGATG TATTTATACC TCAAGATGGG AAGAAGGTGA CGTGGTACTG TTGCGGGCCG 
ACTGTCTATG ATGCGTCTCA CATGGGACAT GCCAGGTCCT ACATCTCCTT CGATATCCTG 
AGGAGGGTGC TGAGGGATTA CTTCCAGTAT GATGTCTTTT ACTGCATGAA CATCACAGAC 
ATTGATGATA AGATCATCAG GCGGGCACGG CAGAACTACC TGTTTGAGCA GTATCGGGAG 
CAGAAACCCC CAGCCACCCA GCTCCTGAAG GATGTCCGTG ATGCCATGAA GCCATTTTCA 
GTCAAGTTAA GTGAGACAAC AGATCCCGAC AAGAGGCAGA TGCTGGAGCG GATCCAGAAC 
TCTGTGAAAC TTGCCACAGA ACCACTGGAA CAGGCTGTGC GATCCAGCCT CTCTGGAGAG 
GAAGTAGACA GCAAAGTACA GGTGTTGCTG GAGGAAGCAA AGGACTTGCT CTCTGACTGG 
CTGGATTCCA CAGGTGGCAG TGAGGTGACT GACAACTCGA TTTTCTCCAA ACTGCCCAAG 
TTCTGGGAAG AAGAGTTCCA CAAAGACATG GAAGCCCTGA ATGTTCTCCC TCCTGATGTC 
CTAACCCGTG TCAGTGAATA TGTGCCAGAA ATTGTGAACT TCGTCCAGAA GATTGTGGAC 
AATGGTTATG GTTATGCTTC AAATGGATCT GTTTACTTCG ACACAGCCAA GTTTGCTGCT 
AGTGAAAAGC ACTCCTATGG GAAGCTGGTG CCTGAGGCCG TTGGGGATCA GAAGGCACTT 
CAGGAAGGGG AAGGTGATCT GAGCATCTCT GCTGACCGCC TTAGTGAGAA ACGCTCTCCC 
AATGACTTTG CCTTGTGGAA GGCCTCCAAG CCAGGCGAGC CATCCTGGCC TTGCCCCTGG 
GGAAAGGGTC GTCCCGGATG GCACATAGAG TGTTCTGCTA TGGCAGGCAC GCTCCTGGGA 
GCCTCAATGG ACATTCATGG TGGAGGGTTT GACCTCCGCT TCCCCCACCA TGACAATGAG 
CTGGCACAGT CGGAGGCCTA CTTTGAAAAT GACTGCTGGG TCAGGTACTT CTTGCACACG 
GGCCACTTGA CGATAGCAGG CTGCAAGATG TCCAAGTCAC TGAAAAACTT TATCACCATT 
AAAGATGCCT TGAAGAAGCA CTCAGCACGG CAGCTGCGAC TGGCATTCCT CATGCACTCA 
TGGAAAGACA CACTGGACTA TTCCAGCAAC ACTATGGAGT CTGCTCTTCA GTATGAAAAA 
TTCATGAACG AGTTTTTCTT AAATGTGAAA GACATCCTCC GAGCCCCTGT AGACATCACT 
GGCCAGTTTG AAAAGTGGGA AGCTGAAGAA GTGGAGCTAA ATAAGAACTT CTATGGCAAG 
AAGACCGCAG TTCACGAAGC CTTGTGTGAT AACATTGACA CCCGGACTGT CATGGAAGAG 
ATGCGGGCTT TGGTCAGTCA GTGCAACCTC TACATGGCAG CCAGGAAGGC TGAACGGAGG 
AGGCCCAACC GGGCTCTGCT GGAGAACATT GCCATGTACC TCACCCACAT GCTGAAGATC 
TTTGGGGCCA TAGAGGAGGA GAGCCCCCTG GGGTTCCCAG TTGGTGGGCC TGGAACCAAC 
CTGAACCTCG AGTCAACAGT CATGCCCTAC CTTCAGGTGT TATCAGAATT CAGAGAGGGA 
GTACGAAAGA TTGCCCGAGA GAAAAAAGTT CTTGAGGTTC TACAGCTCAG TGATGCCCTC 
CGGGATGACA TCCTGCCTGA GCTTGGGGTC CGGTTTGAAG ACCATGAAGG GCTGCCAACA 
GTGGTGAAGT TGGTGGACAG AGACACCTTA CTGAAAGAGA AGGAAGGAAA GAAAAGGGCA 
GCAAAGCTGG CCAAGATGAA GATACCACCC AGTGAGATGT TCCTGTCAGA AGTCAACAAG 
TATTCTAAAT TTGATGAAAA TGGTCTGCCT ACCCACGACA CCGAAGGCAA AGAGCTGAGC 
AAGGGCCAGG CCAAGAAGCT GAAGAAGCTC TTTGAAGCCC AGGAGAAACT GTACAAGGAG 
TATCTGCAAA TGTTACAGAA TGGCAGCCTC CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cars ( NM_013742.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_038770.3 Mus musculus cysteinyl-tRNA synthetase (Cars), transcript variant 1, mRNA.

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CloneID	OMu09873
Clone ID Related Accession (Same CDS sequence)	NM_013742.5
Accession Version	NM_013742.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2496bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-20
Organism	Mus musculus(house mouse)
Product	cysteine--tRNA ligase, cytoplasmic isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY253195.1, BY021374.1, AK135433.1, AJ276796.1 and BC030473.1. On Nov 19, 2011 this sequence version replaced NM_013742.4. Transcript Variant: This variant (1) represents the longer transcript and it encodes the longer protein (isoform 1). ##Evidence-Data-START## Transcript exon combination :: AK033328.1, AK135433.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END##

ATGGCAGGTT CCTCCGCGGA GCAGGCTGCC GACTACAGGT CCATTCTGAG CATTAGTGAC 
GAGGCAGCCA GGGTGCAGGC CCTGGACCAA CACCTCAGCA CGCGTAGCTA TATCCAGGGG 
TACTCCCTAT CCCAGGCAGA TGTGGACGTG TTCAGGCAGC TCTCGGCCCC TCCCGCTGAC 
TCAAGGCTCT TCCACGTGGC TCGGTGGTTC AGGCACATAG AAGCGCTCCT GGGTGGCCCC 
CAAGGCAGAG ACGAGCCCTG CAGGCTTCAA GCAAGTAAAG GCCGGCGAGT GCAGCCTCAG 
TGGTCCCCCC CAGCAGGGAC TGAGCCCTGC AGGCTCCGCC TCTATAATAG CCTCACCCGG 
AACAAGGATG TATTTATACC TCAAGATGGG AAGAAGGTGA CGTGGTACTG TTGCGGGCCG 
ACTGTCTATG ATGCGTCTCA CATGGGACAT GCCAGGTCCT ACATCTCCTT CGATATCCTG 
AGGAGGGTGC TGAGGGATTA CTTCCAGTAT GATGTCTTTT ACTGCATGAA CATCACAGAC 
ATTGATGATA AGATCATCAG GCGGGCACGG CAGAACTACC TGTTTGAGCA GTATCGGGAG 
CAGAAACCCC CAGCCACCCA GCTCCTGAAG GATGTCCGTG ATGCCATGAA GCCATTTTCA 
GTCAAGTTAA GTGAGACAAC AGATCCCGAC AAGAGGCAGA TGCTGGAGCG GATCCAGAAC 
TCTGTGAAAC TTGCCACAGA ACCACTGGAA CAGGCTGTGC GATCCAGCCT CTCTGGAGAG 
GAAGTAGACA GCAAAGTACA GGTGTTGCTG GAGGAAGCAA AGGACTTGCT CTCTGACTGG 
CTGGATTCCA CAGGTGGCAG TGAGGTGACT GACAACTCGA TTTTCTCCAA ACTGCCCAAG 
TTCTGGGAAG AAGAGTTCCA CAAAGACATG GAAGCCCTGA ATGTTCTCCC TCCTGATGTC 
CTAACCCGTG TCAGTGAATA TGTGCCAGAA ATTGTGAACT TCGTCCAGAA GATTGTGGAC 
AATGGTTATG GTTATGCTTC AAATGGATCT GTTTACTTCG ACACAGCCAA GTTTGCTGCT 
AGTGAAAAGC ACTCCTATGG GAAGCTGGTG CCTGAGGCCG TTGGGGATCA GAAGGCACTT 
CAGGAAGGGG AAGGTGATCT GAGCATCTCT GCTGACCGCC TTAGTGAGAA ACGCTCTCCC 
AATGACTTTG CCTTGTGGAA GGCCTCCAAG CCAGGCGAGC CATCCTGGCC TTGCCCCTGG 
GGAAAGGGTC GTCCCGGATG GCACATAGAG TGTTCTGCTA TGGCAGGCAC GCTCCTGGGA 
GCCTCAATGG ACATTCATGG TGGAGGGTTT GACCTCCGCT TCCCCCACCA TGACAATGAG 
CTGGCACAGT CGGAGGCCTA CTTTGAAAAT GACTGCTGGG TCAGGTACTT CTTGCACACG 
GGCCACTTGA CGATAGCAGG CTGCAAGATG TCCAAGTCAC TGAAAAACTT TATCACCATT 
AAAGATGCCT TGAAGAAGCA CTCAGCACGG CAGCTGCGAC TGGCATTCCT CATGCACTCA 
TGGAAAGACA CACTGGACTA TTCCAGCAAC ACTATGGAGT CTGCTCTTCA GTATGAAAAA 
TTCATGAACG AGTTTTTCTT AAATGTGAAA GACATCCTCC GAGCCCCTGT AGACATCACT 
GGCCAGTTTG AAAAGTGGGA AGCTGAAGAA GTGGAGCTAA ATAAGAACTT CTATGGCAAG 
AAGACCGCAG TTCACGAAGC CTTGTGTGAT AACATTGACA CCCGGACTGT CATGGAAGAG 
ATGCGGGCTT TGGTCAGTCA GTGCAACCTC TACATGGCAG CCAGGAAGGC TGAACGGAGG 
AGGCCCAACC GGGCTCTGCT GGAGAACATT GCCATGTACC TCACCCACAT GCTGAAGATC 
TTTGGGGCCA TAGAGGAGGA GAGCCCCCTG GGGTTCCCAG TTGGTGGGCC TGGAACCAAC 
CTGAACCTCG AGTCAACAGT CATGCCCTAC CTTCAGGTGT TATCAGAATT CAGAGAGGGA 
GTACGAAAGA TTGCCCGAGA GAAAAAAGTT CTTGAGGTTC TACAGCTCAG TGATGCCCTC 
CGGGATGACA TCCTGCCTGA GCTTGGGGTC CGGTTTGAAG ACCATGAAGG GCTGCCAACA 
GTGGTGAAGT TGGTGGACAG AGACACCTTA CTGAAAGAGA AGGAAGGAAA GAAAAGGGCT 
GAAGAAGAGA AGAGGAGGAA GAAAGAGGAG GCGGCCAGAA AGAAACAGGA GCAAGAAGCA 
GCAAAGCTGG CCAAGATGAA GATACCACCC AGTGAGATGT TCCTGTCAGA AGTCAACAAG 
TATTCTAAAT TTGATGAAAA TGGTCTGCCT ACCCACGACA CCGAAGGCAA AGAGCTGAGC 
AAGGGCCAGG CCAAGAAGCT GAAGAAGCTC TTTGAAGCCC AGGAGAAACT GTACAAGGAG 
TATCTGCAAA TGTTACAGAA TGGCAGCCTC CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038770.3
CDS	85..2580
Translation	MAGSSAEQAADYRSILSISDEAARVQALDQHLSTRSYIQGYSLSQADVDVFRQLSAPPADSRLFHVARWFRHIEALLGGPQGRDEPCRLQASKGRRVQPQWSPPAGTEPCRLRLYNSLTRNKDVFIPQDGKKVTWYCCGPTVYDASHMGHARSYISFDILRRVLRDYFQYDVFYCMNITDIDDKIIRRARQNYLFEQYREQKPPATQLLKDVRDAMKPFSVKLSETTDPDKRQMLERIQNSVKLATEPLEQAVRSSLSGEEVDSKVQVLLEEAKDLLSDWLDSTGGSEVTDNSIFSKLPKFWEEEFHKDMEALNVLPPDVLTRVSEYVPEIVNFVQKIVDNGYGYASNGSVYFDTAKFAASEKHSYGKLVPEAVGDQKALQEGEGDLSISADRLSEKRSPNDFALWKASKPGEPSWPCPWGKGRPGWHIECSAMAGTLLGASMDIHGGGFDLRFPHHDNELAQSEAYFENDCWVRYFLHTGHLTIAGCKMSKSLKNFITIKDALKKHSARQLRLAFLMHSWKDTLDYSSNTMESALQYEKFMNEFFLNVKDILRAPVDITGQFEKWEAEEVELNKNFYGKKTAVHEALCDNIDTRTVMEEMRALVSQCNLYMAARKAERRRPNRALLENIAMYLTHMLKIFGAIEEESPLGFPVGGPGTNLNLESTVMPYLQVLSEFREGVRKIAREKKVLEVLQLSDALRDDILPELGVRFEDHEGLPTVVKLVDRDTLLKEKEGKKRAEEEKRRKKEEAARKKQEQEAAKLAKMKIPPSEMFLSEVNKYSKFDENGLPTHDTEGKELSKGQAKKLKKLFEAQEKLYKEYLQMLQNGSLQ

Target ORF information:

RefSeq Version	NM_013742.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus cysteinyl-tRNA synthetase (Cars), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013742.5

ORF Insert Sequence:

ATGGCAGGTT CCTCCGCGGA GCAGGCTGCC GACTACAGGT CCATTCTGAG CATTAGTGAC 
GAGGCAGCCA GGGTGCAGGC CCTGGACCAA CACCTCAGCA CGCGTAGCTA TATCCAGGGG 
TACTCCCTAT CCCAGGCAGA TGTGGACGTG TTCAGGCAGC TCTCGGCCCC TCCCGCTGAC 
TCAAGGCTCT TCCACGTGGC TCGGTGGTTC AGGCACATAG AAGCGCTCCT GGGTGGCCCC 
CAAGGCAGAG ACGAGCCCTG CAGGCTTCAA GCAAGTAAAG GCCGGCGAGT GCAGCCTCAG 
TGGTCCCCCC CAGCAGGGAC TGAGCCCTGC AGGCTCCGCC TCTATAATAG CCTCACCCGG 
AACAAGGATG TATTTATACC TCAAGATGGG AAGAAGGTGA CGTGGTACTG TTGCGGGCCG 
ACTGTCTATG ATGCGTCTCA CATGGGACAT GCCAGGTCCT ACATCTCCTT CGATATCCTG 
AGGAGGGTGC TGAGGGATTA CTTCCAGTAT GATGTCTTTT ACTGCATGAA CATCACAGAC 
ATTGATGATA AGATCATCAG GCGGGCACGG CAGAACTACC TGTTTGAGCA GTATCGGGAG 
CAGAAACCCC CAGCCACCCA GCTCCTGAAG GATGTCCGTG ATGCCATGAA GCCATTTTCA 
GTCAAGTTAA GTGAGACAAC AGATCCCGAC AAGAGGCAGA TGCTGGAGCG GATCCAGAAC 
TCTGTGAAAC TTGCCACAGA ACCACTGGAA CAGGCTGTGC GATCCAGCCT CTCTGGAGAG 
GAAGTAGACA GCAAAGTACA GGTGTTGCTG GAGGAAGCAA AGGACTTGCT CTCTGACTGG 
CTGGATTCCA CAGGTGGCAG TGAGGTGACT GACAACTCGA TTTTCTCCAA ACTGCCCAAG 
TTCTGGGAAG AAGAGTTCCA CAAAGACATG GAAGCCCTGA ATGTTCTCCC TCCTGATGTC 
CTAACCCGTG TCAGTGAATA TGTGCCAGAA ATTGTGAACT TCGTCCAGAA GATTGTGGAC 
AATGGTTATG GTTATGCTTC AAATGGATCT GTTTACTTCG ACACAGCCAA GTTTGCTGCT 
AGTGAAAAGC ACTCCTATGG GAAGCTGGTG CCTGAGGCCG TTGGGGATCA GAAGGCACTT 
CAGGAAGGGG AAGGTGATCT GAGCATCTCT GCTGACCGCC TTAGTGAGAA ACGCTCTCCC 
AATGACTTTG CCTTGTGGAA GGCCTCCAAG CCAGGCGAGC CATCCTGGCC TTGCCCCTGG 
GGAAAGGGTC GTCCCGGATG GCACATAGAG TGTTCTGCTA TGGCAGGCAC GCTCCTGGGA 
GCCTCAATGG ACATTCATGG TGGAGGGTTT GACCTCCGCT TCCCCCACCA TGACAATGAG 
CTGGCACAGT CGGAGGCCTA CTTTGAAAAT GACTGCTGGG TCAGGTACTT CTTGCACACG 
GGCCACTTGA CGATAGCAGG CTGCAAGATG TCCAAGTCAC TGAAAAACTT TATCACCATT 
AAAGATGCCT TGAAGAAGCA CTCAGCACGG CAGCTGCGAC TGGCATTCCT CATGCACTCA 
TGGAAAGACA CACTGGACTA TTCCAGCAAC ACTATGGAGT CTGCTCTTCA GTATGAAAAA 
TTCATGAACG AGTTTTTCTT AAATGTGAAA GACATCCTCC GAGCCCCTGT AGACATCACT 
GGCCAGTTTG AAAAGTGGGA AGCTGAAGAA GTGGAGCTAA ATAAGAACTT CTATGGCAAG 
AAGACCGCAG TTCACGAAGC CTTGTGTGAT AACATTGACA CCCGGACTGT CATGGAAGAG 
ATGCGGGCTT TGGTCAGTCA GTGCAACCTC TACATGGCAG CCAGGAAGGC TGAACGGAGG 
AGGCCCAACC GGGCTCTGCT GGAGAACATT GCCATGTACC TCACCCACAT GCTGAAGATC 
TTTGGGGCCA TAGAGGAGGA GAGCCCCCTG GGGTTCCCAG TTGGTGGGCC TGGAACCAAC 
CTGAACCTCG AGTCAACAGT CATGCCCTAC CTTCAGGTGT TATCAGAATT CAGAGAGGGA 
GTACGAAAGA TTGCCCGAGA GAAAAAAGTT CTTGAGGTTC TACAGCTCAG TGATGCCCTC 
CGGGATGACA TCCTGCCTGA GCTTGGGGTC CGGTTTGAAG ACCATGAAGG GCTGCCAACA 
GTGGTGAAGT TGGTGGACAG AGACACCTTA CTGAAAGAGA AGGAAGGAAA GAAAAGGGCT 
GAAGAAGAGA AGAGGAGGAA GAAAGAGGAG GCGGCCAGAA AGAAACAGGA GCAAGAAGCA 
GCAAAGCTGG CCAAGATGAA GATACCACCC AGTGAGATGT TCCTGTCAGA AGTCAACAAG 
TATTCTAAAT TTGATGAAAA TGGTCTGCCT ACCCACGACA CCGAAGGCAA AGAGCTGAGC 
AAGGGCCAGG CCAAGAAGCT GAAGAAGCTC TTTGAAGCCC AGGAGAAACT GTACAAGGAG 
TATCTGCAAA TGTTACAGAA TGGCAGCCTC CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cars ( XM_011242001.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011240303.1 Mus musculus cysteinyl-tRNA synthetase (Cars), transcript variant X4, mRNA.

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CloneID	OMu69278
Clone ID Related Accession (Same CDS sequence)	XM_011242001.1 , XM_011242001.2 , XM_030242631.1 , XM_011242000.1
Accession Version	XM_011242001.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	cysteine--tRNA ligase, cytoplasmic isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011242001.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACATCA CAGACATTGA TGATAAGATC ATCAGGCGGG CACGGCAGAA CTACCTGTTT 
GAGCAGTATC GGGAGCAGAA ACCCCCAGCC ACCCAGCTCC TGAAGGATGT CCGTGATGCC 
ATGAAGCCAT TTTCAGTCAA GTTAAGTGAG ACAACAGATC CCGACAAGAG GCAGATGCTG 
GAGCGGATCC AGAACTCTGT GAAACTTGCC ACAGAACCAC TGGAACAGGC TGTGCGATCC 
AGCCTCTCTG GAGAGGAAGT AGACAGCAAA GTACAGGTGT TGCTGGAGGA AGCAAAGGAC 
TTGCTCTCTG ACTGGCTGGA TTCCACAGGT GGCAGTGAGG TGACTGACAA CTCGATTTTC 
TCCAAACTGC CCAAGTTCTG GGAAGAAGAG TTCCACAAAG ACATGGAAGC CCTGAATGTT 
CTCCCTCCTG ATGTCCTAAC CCGTGTCAGT GAATATGTGC CAGAAATTGT GAACTTCGTC 
CAGAAGATTG TGGACAATGG TTATGGTTAT GCTTCAAATG GATCTGTTTA CTTCGACACA 
GCCAAGTTTG CTGCTAGTGA AAAGCACTCC TATGGGAAGC TGGTGCCTGA GGCCGTTGGG 
GATCAGAAGG CACTTCAGGA AGGGGAAGGT GATCTGAGCA TCTCTGCTGA CCGCCTTAGT 
GAGAAACGCT CTCCCAATGA CTTTGCCTTG TGGAAGGCCT CCAAGCCAGG CGAGCCATCC 
TGGCCTTGCC CCTGGGGAAA GGGTCGTCCC GGATGGCACA TAGAGTGTTC TGCTATGGCA 
GGCACGCTCC TGGGAGCCTC AATGGACATT CATGGTGGAG GGTTTGACCT CCGCTTCCCC 
CACCATGACA ATGAGCTGGC ACAGTCGGAG GCCTACTTTG AAAATGACTG CTGGGTCAGG 
TACTTCTTGC ACACGGGCCA CTTGACGATA GCAGGCTGCA AGATGTCCAA GTCACTGAAA 
AACTTTATCA CCATTAAAGA TGCCTTGAAG AAGCACTCAG CACGGCAGCT GCGACTGGCA 
TTCCTCATGC ACTCATGGAA AGACACACTG GACTATTCCA GCAACACTAT GGAGTCTGCT 
CTTCAGTATG AAAAATTCAT GAACGAGTTT TTCTTAAATG TGAAAGACAT CCTCCGAGCC 
CCTGTAGACA TCACTGGCCA GTTTGAAAAG TGGGAAGCTG AAGAAGTGGA GCTAAATAAG 
AACTTCTATG GCAAGAAGAC CGCAGTTCAC GAAGCCTTGT GTGATAACAT TGACACCCGG 
ACTGTCATGG AAGAGATGCG GGCTTTGGTC AGTCAGTGCA ACCTCTACAT GGCAGCCAGG 
AAGGCTGAAC GGAGGAGGCC CAACCGGGCT CTGCTGGAGA ACATTGCCAT GTACCTCACC 
CACATGCTGA AGATCTTTGG GGCCATAGAG GAGGAGAGCC CCCTGGGGTT CCCAGTTGGT 
GGGCCTGGAA CCAACCTGAA CCTCGAGTCA ACAGTCATGC CCTACCTTCA GGTGTTATCA 
GAATTCAGAG AGGGAGTACG AAAGATTGCC CGAGAGAAAA AAGTTCTTGA GGTTCTACAG 
CTCAGTGATG CCCTCCGGGA TGACATCCTG CCTGAGCTTG GGGTCCGGTT TGAAGACCAT 
GAAGGGCTGC CAACAGTGGT GAAGTTGGTG GACAGAGACA CCTTACTGAA AGAGAAGGAA 
GGAAAGAAAA GGGCTGAAGA AGAGAAGAGG AGGAAGAAAG AGGAGGCGGC CAGAAAGAAA 
CAGGAGCAAG AAGCAGCAAA GCTGGCCAAG ATGAAGATAC CACCCAGTGA GATGTTCCTG 
TCAGAAGTCA ACAAGTATTC TAAATTTGAT GAAAATGGTC TGCCTACCCA CGACACCGAA 
GGCAAAGAGC TGAGCAAGGG CCAGGCCAAG AAGCTGAAGA AGCTCTTTGA AGCCCAGGAG 
AAACTGTACA AGGAGTATCT GCAAATGTTA CAGAATGGCA GCCTCCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011240303.1
CDS	268..2238
Translation	MNITDIDDKIIRRARQNYLFEQYREQKPPATQLLKDVRDAMKPFSVKLSETTDPDKRQMLERIQNSVKLATEPLEQAVRSSLSGEEVDSKVQVLLEEAKDLLSDWLDSTGGSEVTDNSIFSKLPKFWEEEFHKDMEALNVLPPDVLTRVSEYVPEIVNFVQKIVDNGYGYASNGSVYFDTAKFAASEKHSYGKLVPEAVGDQKALQEGEGDLSISADRLSEKRSPNDFALWKASKPGEPSWPCPWGKGRPGWHIECSAMAGTLLGASMDIHGGGFDLRFPHHDNELAQSEAYFENDCWVRYFLHTGHLTIAGCKMSKSLKNFITIKDALKKHSARQLRLAFLMHSWKDTLDYSSNTMESALQYEKFMNEFFLNVKDILRAPVDITGQFEKWEAEEVELNKNFYGKKTAVHEALCDNIDTRTVMEEMRALVSQCNLYMAARKAERRRPNRALLENIAMYLTHMLKIFGAIEEESPLGFPVGGPGTNLNLESTVMPYLQVLSEFREGVRKIAREKKVLEVLQLSDALRDDILPELGVRFEDHEGLPTVVKLVDRDTLLKEKEGKKRAEEEKRRKKEEAARKKQEQEAAKLAKMKIPPSEMFLSEVNKYSKFDENGLPTHDTEGKELSKGQAKKLKKLFEAQEKLYKEYLQMLQNGSLQ

Target ORF information:

RefSeq Version	XM_011242001.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus cysteinyl-tRNA synthetase (Cars), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011242001.2

ORF Insert Sequence:

ATGAACATCA CAGACATTGA TGATAAGATC ATCAGGCGGG CACGGCAGAA CTACCTGTTT 
GAGCAGTATC GGGAGCAGAA ACCCCCAGCC ACCCAGCTCC TGAAGGATGT CCGTGATGCC 
ATGAAGCCAT TTTCAGTCAA GTTAAGTGAG ACAACAGATC CCGACAAGAG GCAGATGCTG 
GAGCGGATCC AGAACTCTGT GAAACTTGCC ACAGAACCAC TGGAACAGGC TGTGCGATCC 
AGCCTCTCTG GAGAGGAAGT AGACAGCAAA GTACAGGTGT TGCTGGAGGA AGCAAAGGAC 
TTGCTCTCTG ACTGGCTGGA TTCCACAGGT GGCAGTGAGG TGACTGACAA CTCGATTTTC 
TCCAAACTGC CCAAGTTCTG GGAAGAAGAG TTCCACAAAG ACATGGAAGC CCTGAATGTT 
CTCCCTCCTG ATGTCCTAAC CCGTGTCAGT GAATATGTGC CAGAAATTGT GAACTTCGTC 
CAGAAGATTG TGGACAATGG TTATGGTTAT GCTTCAAATG GATCTGTTTA CTTCGACACA 
GCCAAGTTTG CTGCTAGTGA AAAGCACTCC TATGGGAAGC TGGTGCCTGA GGCCGTTGGG 
GATCAGAAGG CACTTCAGGA AGGGGAAGGT GATCTGAGCA TCTCTGCTGA CCGCCTTAGT 
GAGAAACGCT CTCCCAATGA CTTTGCCTTG TGGAAGGCCT CCAAGCCAGG CGAGCCATCC 
TGGCCTTGCC CCTGGGGAAA GGGTCGTCCC GGATGGCACA TAGAGTGTTC TGCTATGGCA 
GGCACGCTCC TGGGAGCCTC AATGGACATT CATGGTGGAG GGTTTGACCT CCGCTTCCCC 
CACCATGACA ATGAGCTGGC ACAGTCGGAG GCCTACTTTG AAAATGACTG CTGGGTCAGG 
TACTTCTTGC ACACGGGCCA CTTGACGATA GCAGGCTGCA AGATGTCCAA GTCACTGAAA 
AACTTTATCA CCATTAAAGA TGCCTTGAAG AAGCACTCAG CACGGCAGCT GCGACTGGCA 
TTCCTCATGC ACTCATGGAA AGACACACTG GACTATTCCA GCAACACTAT GGAGTCTGCT 
CTTCAGTATG AAAAATTCAT GAACGAGTTT TTCTTAAATG TGAAAGACAT CCTCCGAGCC 
CCTGTAGACA TCACTGGCCA GTTTGAAAAG TGGGAAGCTG AAGAAGTGGA GCTAAATAAG 
AACTTCTATG GCAAGAAGAC CGCAGTTCAC GAAGCCTTGT GTGATAACAT TGACACCCGG 
ACTGTCATGG AAGAGATGCG GGCTTTGGTC AGTCAGTGCA ACCTCTACAT GGCAGCCAGG 
AAGGCTGAAC GGAGGAGGCC CAACCGGGCT CTGCTGGAGA ACATTGCCAT GTACCTCACC 
CACATGCTGA AGATCTTTGG GGCCATAGAG GAGGAGAGCC CCCTGGGGTT CCCAGTTGGT 
GGGCCTGGAA CCAACCTGAA CCTCGAGTCA ACAGTCATGC CCTACCTTCA GGTGTTATCA 
GAATTCAGAG AGGGAGTACG AAAGATTGCC CGAGAGAAAA AAGTTCTTGA GGTTCTACAG 
CTCAGTGATG CCCTCCGGGA TGACATCCTG CCTGAGCTTG GGGTCCGGTT TGAAGACCAT 
GAAGGGCTGC CAACAGTGGT GAAGTTGGTG GACAGAGACA CCTTACTGAA AGAGAAGGAA 
GGAAAGAAAA GGGCTGAAGA AGAGAAGAGG AGGAAGAAAG AGGAGGCGGC CAGAAAGAAA 
CAGGAGCAAG AAGCAGCAAA GCTGGCCAAG ATGAAGATAC CACCCAGTGA GATGTTCCTG 
TCAGAAGTCA ACAAGTATTC TAAATTTGAT GAAAATGGTC TGCCTACCCA CGACACCGAA 
GGCAAAGAGC TGAGCAAGGG CCAGGCCAAG AAGCTGAAGA AGCTCTTTGA AGCCCAGGAG 
AAACTGTACA AGGAGTATCT GCAAATGTTA CAGAATGGCA GCCTCCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cars ( XM_030242631.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030098491.1 Mus musculus cysteinyl-tRNA synthetase (Cars), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu69278
Clone ID Related Accession (Same CDS sequence)	XM_011242001.1 , XM_011242001.2 , XM_030242631.1 , XM_011242000.1
Accession Version	XM_030242631.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	cysteine--tRNA ligase, cytoplasmic isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACATCA CAGACATTGA TGATAAGATC ATCAGGCGGG CACGGCAGAA CTACCTGTTT 
GAGCAGTATC GGGAGCAGAA ACCCCCAGCC ACCCAGCTCC TGAAGGATGT CCGTGATGCC 
ATGAAGCCAT TTTCAGTCAA GTTAAGTGAG ACAACAGATC CCGACAAGAG GCAGATGCTG 
GAGCGGATCC AGAACTCTGT GAAACTTGCC ACAGAACCAC TGGAACAGGC TGTGCGATCC 
AGCCTCTCTG GAGAGGAAGT AGACAGCAAA GTACAGGTGT TGCTGGAGGA AGCAAAGGAC 
TTGCTCTCTG ACTGGCTGGA TTCCACAGGT GGCAGTGAGG TGACTGACAA CTCGATTTTC 
TCCAAACTGC CCAAGTTCTG GGAAGAAGAG TTCCACAAAG ACATGGAAGC CCTGAATGTT 
CTCCCTCCTG ATGTCCTAAC CCGTGTCAGT GAATATGTGC CAGAAATTGT GAACTTCGTC 
CAGAAGATTG TGGACAATGG TTATGGTTAT GCTTCAAATG GATCTGTTTA CTTCGACACA 
GCCAAGTTTG CTGCTAGTGA AAAGCACTCC TATGGGAAGC TGGTGCCTGA GGCCGTTGGG 
GATCAGAAGG CACTTCAGGA AGGGGAAGGT GATCTGAGCA TCTCTGCTGA CCGCCTTAGT 
GAGAAACGCT CTCCCAATGA CTTTGCCTTG TGGAAGGCCT CCAAGCCAGG CGAGCCATCC 
TGGCCTTGCC CCTGGGGAAA GGGTCGTCCC GGATGGCACA TAGAGTGTTC TGCTATGGCA 
GGCACGCTCC TGGGAGCCTC AATGGACATT CATGGTGGAG GGTTTGACCT CCGCTTCCCC 
CACCATGACA ATGAGCTGGC ACAGTCGGAG GCCTACTTTG AAAATGACTG CTGGGTCAGG 
TACTTCTTGC ACACGGGCCA CTTGACGATA GCAGGCTGCA AGATGTCCAA GTCACTGAAA 
AACTTTATCA CCATTAAAGA TGCCTTGAAG AAGCACTCAG CACGGCAGCT GCGACTGGCA 
TTCCTCATGC ACTCATGGAA AGACACACTG GACTATTCCA GCAACACTAT GGAGTCTGCT 
CTTCAGTATG AAAAATTCAT GAACGAGTTT TTCTTAAATG TGAAAGACAT CCTCCGAGCC 
CCTGTAGACA TCACTGGCCA GTTTGAAAAG TGGGAAGCTG AAGAAGTGGA GCTAAATAAG 
AACTTCTATG GCAAGAAGAC CGCAGTTCAC GAAGCCTTGT GTGATAACAT TGACACCCGG 
ACTGTCATGG AAGAGATGCG GGCTTTGGTC AGTCAGTGCA ACCTCTACAT GGCAGCCAGG 
AAGGCTGAAC GGAGGAGGCC CAACCGGGCT CTGCTGGAGA ACATTGCCAT GTACCTCACC 
CACATGCTGA AGATCTTTGG GGCCATAGAG GAGGAGAGCC CCCTGGGGTT CCCAGTTGGT 
GGGCCTGGAA CCAACCTGAA CCTCGAGTCA ACAGTCATGC CCTACCTTCA GGTGTTATCA 
GAATTCAGAG AGGGAGTACG AAAGATTGCC CGAGAGAAAA AAGTTCTTGA GGTTCTACAG 
CTCAGTGATG CCCTCCGGGA TGACATCCTG CCTGAGCTTG GGGTCCGGTT TGAAGACCAT 
GAAGGGCTGC CAACAGTGGT GAAGTTGGTG GACAGAGACA CCTTACTGAA AGAGAAGGAA 
GGAAAGAAAA GGGCTGAAGA AGAGAAGAGG AGGAAGAAAG AGGAGGCGGC CAGAAAGAAA 
CAGGAGCAAG AAGCAGCAAA GCTGGCCAAG ATGAAGATAC CACCCAGTGA GATGTTCCTG 
TCAGAAGTCA ACAAGTATTC TAAATTTGAT GAAAATGGTC TGCCTACCCA CGACACCGAA 
GGCAAAGAGC TGAGCAAGGG CCAGGCCAAG AAGCTGAAGA AGCTCTTTGA AGCCCAGGAG 
AAACTGTACA AGGAGTATCT GCAAATGTTA CAGAATGGCA GCCTCCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030098491.1
CDS	482..2452
Translation	MNITDIDDKIIRRARQNYLFEQYREQKPPATQLLKDVRDAMKPFSVKLSETTDPDKRQMLERIQNSVKLATEPLEQAVRSSLSGEEVDSKVQVLLEEAKDLLSDWLDSTGGSEVTDNSIFSKLPKFWEEEFHKDMEALNVLPPDVLTRVSEYVPEIVNFVQKIVDNGYGYASNGSVYFDTAKFAASEKHSYGKLVPEAVGDQKALQEGEGDLSISADRLSEKRSPNDFALWKASKPGEPSWPCPWGKGRPGWHIECSAMAGTLLGASMDIHGGGFDLRFPHHDNELAQSEAYFENDCWVRYFLHTGHLTIAGCKMSKSLKNFITIKDALKKHSARQLRLAFLMHSWKDTLDYSSNTMESALQYEKFMNEFFLNVKDILRAPVDITGQFEKWEAEEVELNKNFYGKKTAVHEALCDNIDTRTVMEEMRALVSQCNLYMAARKAERRRPNRALLENIAMYLTHMLKIFGAIEEESPLGFPVGGPGTNLNLESTVMPYLQVLSEFREGVRKIAREKKVLEVLQLSDALRDDILPELGVRFEDHEGLPTVVKLVDRDTLLKEKEGKKRAEEEKRRKKEEAARKKQEQEAAKLAKMKIPPSEMFLSEVNKYSKFDENGLPTHDTEGKELSKGQAKKLKKLFEAQEKLYKEYLQMLQNGSLQ

Target ORF information:

RefSeq Version	XM_030242631.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus cysteinyl-tRNA synthetase (Cars), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030242631.1

ORF Insert Sequence:

ATGAACATCA CAGACATTGA TGATAAGATC ATCAGGCGGG CACGGCAGAA CTACCTGTTT 
GAGCAGTATC GGGAGCAGAA ACCCCCAGCC ACCCAGCTCC TGAAGGATGT CCGTGATGCC 
ATGAAGCCAT TTTCAGTCAA GTTAAGTGAG ACAACAGATC CCGACAAGAG GCAGATGCTG 
GAGCGGATCC AGAACTCTGT GAAACTTGCC ACAGAACCAC TGGAACAGGC TGTGCGATCC 
AGCCTCTCTG GAGAGGAAGT AGACAGCAAA GTACAGGTGT TGCTGGAGGA AGCAAAGGAC 
TTGCTCTCTG ACTGGCTGGA TTCCACAGGT GGCAGTGAGG TGACTGACAA CTCGATTTTC 
TCCAAACTGC CCAAGTTCTG GGAAGAAGAG TTCCACAAAG ACATGGAAGC CCTGAATGTT 
CTCCCTCCTG ATGTCCTAAC CCGTGTCAGT GAATATGTGC CAGAAATTGT GAACTTCGTC 
CAGAAGATTG TGGACAATGG TTATGGTTAT GCTTCAAATG GATCTGTTTA CTTCGACACA 
GCCAAGTTTG CTGCTAGTGA AAAGCACTCC TATGGGAAGC TGGTGCCTGA GGCCGTTGGG 
GATCAGAAGG CACTTCAGGA AGGGGAAGGT GATCTGAGCA TCTCTGCTGA CCGCCTTAGT 
GAGAAACGCT CTCCCAATGA CTTTGCCTTG TGGAAGGCCT CCAAGCCAGG CGAGCCATCC 
TGGCCTTGCC CCTGGGGAAA GGGTCGTCCC GGATGGCACA TAGAGTGTTC TGCTATGGCA 
GGCACGCTCC TGGGAGCCTC AATGGACATT CATGGTGGAG GGTTTGACCT CCGCTTCCCC 
CACCATGACA ATGAGCTGGC ACAGTCGGAG GCCTACTTTG AAAATGACTG CTGGGTCAGG 
TACTTCTTGC ACACGGGCCA CTTGACGATA GCAGGCTGCA AGATGTCCAA GTCACTGAAA 
AACTTTATCA CCATTAAAGA TGCCTTGAAG AAGCACTCAG CACGGCAGCT GCGACTGGCA 
TTCCTCATGC ACTCATGGAA AGACACACTG GACTATTCCA GCAACACTAT GGAGTCTGCT 
CTTCAGTATG AAAAATTCAT GAACGAGTTT TTCTTAAATG TGAAAGACAT CCTCCGAGCC 
CCTGTAGACA TCACTGGCCA GTTTGAAAAG TGGGAAGCTG AAGAAGTGGA GCTAAATAAG 
AACTTCTATG GCAAGAAGAC CGCAGTTCAC GAAGCCTTGT GTGATAACAT TGACACCCGG 
ACTGTCATGG AAGAGATGCG GGCTTTGGTC AGTCAGTGCA ACCTCTACAT GGCAGCCAGG 
AAGGCTGAAC GGAGGAGGCC CAACCGGGCT CTGCTGGAGA ACATTGCCAT GTACCTCACC 
CACATGCTGA AGATCTTTGG GGCCATAGAG GAGGAGAGCC CCCTGGGGTT CCCAGTTGGT 
GGGCCTGGAA CCAACCTGAA CCTCGAGTCA ACAGTCATGC CCTACCTTCA GGTGTTATCA 
GAATTCAGAG AGGGAGTACG AAAGATTGCC CGAGAGAAAA AAGTTCTTGA GGTTCTACAG 
CTCAGTGATG CCCTCCGGGA TGACATCCTG CCTGAGCTTG GGGTCCGGTT TGAAGACCAT 
GAAGGGCTGC CAACAGTGGT GAAGTTGGTG GACAGAGACA CCTTACTGAA AGAGAAGGAA 
GGAAAGAAAA GGGCTGAAGA AGAGAAGAGG AGGAAGAAAG AGGAGGCGGC CAGAAAGAAA 
CAGGAGCAAG AAGCAGCAAA GCTGGCCAAG ATGAAGATAC CACCCAGTGA GATGTTCCTG 
TCAGAAGTCA ACAAGTATTC TAAATTTGAT GAAAATGGTC TGCCTACCCA CGACACCGAA 
GGCAAAGAGC TGAGCAAGGG CCAGGCCAAG AAGCTGAAGA AGCTCTTTGA AGCCCAGGAG 
AAACTGTACA AGGAGTATCT GCAAATGTTA CAGAATGGCA GCCTCCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cars ( XM_017322295.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017177784.1 Mus musculus cysteinyl-tRNA synthetase (Cars), transcript variant X3, mRNA.

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CloneID	OMu82109
Clone ID Related Accession (Same CDS sequence)	XM_017322295.1
Accession Version	XM_017322295.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	cysteine--tRNA ligase, cytoplasmic isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGTT CCTCCGCGGA GCAGGCTGCC GACTACAGGT CCATTCTGAG CATTAGTGAC 
GAGGCAGCCA GGGTGCAGGC CCTGGACCAA CACCTCAGCA CGCGTAGCTA TATCCAGGGG 
TACTCCCTAT CCCAGGCAGA TGTGGACGTG TTCAGGCAGC TCTCGGCCCC TCCCGCTGAC 
TCAAGGCTCT TCCACGTGGC TCGGTGGTTC AGGCACATAG AAGCGCTCCT GGGTGGCCCC 
CAAGGCAGAG ACGAGCCCTG CAGGCTTCAA GCAAGTAAAG GCCGGCGAGT GCAGCCTCAG 
TGGTCCCCCC CAGCAGGGAC TGAGCCCTGC AGGCTCCGCC TCTATAATAG CCTCACCCGG 
AACAAGGATG TATTTATACC TCAAGATGGG AAGAAGGTGA CGTGGTACTG TTGCGGGCCG 
ACTGTCTATG ATGCGTCTCA CATGGGACAT GCCAGGTCCT ACATCTCCTT CGATATCCTG 
AGGAGGGTGC TGAGGGATTA CTTCCAGTAT GATGTCTTTT ACTGCATGAA CATCACAGAC 
ATTGATGATA AGATCATCAG GCGGGCACGG CAGAACTACC TGTTTGAGCA GTATCGGGAG 
CAGAAACCCC CAGCCACCCA GCTCCTGAAG GATGTCCGTG ATGCCATGAA GCCATTTTCA 
GTCAAGTTAA GTGAGACAAC AGATCCCGAC AAGAGGCAGA TGCTGGAGCG GATCCAGAAC 
TCTGTGAAAC TTGCCACAGA ACCACTGGAA CAGGCTGTGC GATCCAGCCT CTCTGGAGAG 
GAAGTAGACA GCAAAGTACA GGTGTTGCTG GAGGAAGCAA AGGACTTGCT CTCTGACTGG 
CTGGATTCCA CAGGTGGCAG TGAGGTGACT GACAACTCGA TTTTCTCCAA ACTGCCCAAG 
TTCTGGGAAG AAGAGTTCCA CAAAGACATG GAAGCCCTGA ATGTTCTCCC TCCTGATGTC 
CTAACCCGTG TCAGTGAATA TGTGCCAGAA ATTGTGAACT TCGTCCAGAA GATTGTGGAC 
AATGGTTATG GTTATGCTTC AAATGGATCT GTTTACTTCG ACACAGCCAA GTTTGCTGCT 
AGTGAAAAGC ACTCCTATGG GAAGCTGGTG CCTGAGGCCG TTGGGGATCA GAAGGCACTT 
CAGGAAGGGG AAGGTGATCT GAGCATCTCT GCTGACCGCC TTAGTGAGAA ACGCTCTCCC 
AATGACTTTG CCTTGTGGAA GGCCTCCAAG CCAGGCGAGC CATCCTGGCC TTGCCCCTGG 
GGAAAGGGTC GTCCCGGATG GCACATAGAG TGTTCTGCTA TGGCAGGCAC GCTCCTGGGA 
GCCTCAATGG ACATTCATGG TGGAGGGTTT GACCTCCGCT TCCCCCACCA TGACAATGAG 
CTGGCACAGT CGGAGGCCTA CTTTGAAAAT GACTGCTGGG TCAGGTACTT CTTGCACACG 
GGCCACTTGA CGATAGCAGG CTGCAAGATG TCCAAGTCAC TGAAAAACTT TATCACCATT 
AAAGATGCCT TGAAGAAGCA CTCAGCACGG CAGCTGCGAC TGGCATTCCT CATGCACTCA 
TGGAAAGACA CACTGGACTA TTCCAGCAAC ACTATGGAGT CTGCTCTTCA GTATGAAAAA 
TTCATGAACG AGTTTTTCTT AAATGTGAAA GACATCCTCC GAGCCCCTGT AGACATCACT 
GGCCAGTTTG AAAAGTGGGA AGCTGAAGAA GTGGAGCTAA ATAAGAACTT CTATGGCAAG 
AAGACCGCAG TTCACGAAGC CTTGTGTGAT AACATTGACA CCCGGACTGT CATGGAAGAG 
ATGCGGGCTT TGGTCAGTCA GTGCAACCTC TACATGGCAG CCAGGAAGGC TGAACGGAGG 
AGGCCCAACC GGGCTCTGCT GGAGAACATT GCCATGTACC TCACCCACAT GCTGAAGATC 
TTTGGGGCCA TAGAGGAGGA GAGCCCCCTG GGGTTCCCAG TTGGTGGGCC TGGAACCAAC 
CTGAACCTCG AGTCAACAGT CATGCCCTAC CTTCAGGTGT TATCAGAATT CAGAGAGGGA 
GTACGAAAGA TTGCCCGAGA GAAAAAAAGT TCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017177784.1
CDS	83..2158
Translation	MAGSSAEQAADYRSILSISDEAARVQALDQHLSTRSYIQGYSLSQADVDVFRQLSAPPADSRLFHVARWFRHIEALLGGPQGRDEPCRLQASKGRRVQPQWSPPAGTEPCRLRLYNSLTRNKDVFIPQDGKKVTWYCCGPTVYDASHMGHARSYISFDILRRVLRDYFQYDVFYCMNITDIDDKIIRRARQNYLFEQYREQKPPATQLLKDVRDAMKPFSVKLSETTDPDKRQMLERIQNSVKLATEPLEQAVRSSLSGEEVDSKVQVLLEEAKDLLSDWLDSTGGSEVTDNSIFSKLPKFWEEEFHKDMEALNVLPPDVLTRVSEYVPEIVNFVQKIVDNGYGYASNGSVYFDTAKFAASEKHSYGKLVPEAVGDQKALQEGEGDLSISADRLSEKRSPNDFALWKASKPGEPSWPCPWGKGRPGWHIECSAMAGTLLGASMDIHGGGFDLRFPHHDNELAQSEAYFENDCWVRYFLHTGHLTIAGCKMSKSLKNFITIKDALKKHSARQLRLAFLMHSWKDTLDYSSNTMESALQYEKFMNEFFLNVKDILRAPVDITGQFEKWEAEEVELNKNFYGKKTAVHEALCDNIDTRTVMEEMRALVSQCNLYMAARKAERRRPNRALLENIAMYLTHMLKIFGAIEEESPLGFPVGGPGTNLNLESTVMPYLQVLSEFREGVRKIAREKKSS

Target ORF information:

RefSeq Version	XM_017322295.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus cysteinyl-tRNA synthetase (Cars), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017322295.1

ORF Insert Sequence:

ATGGCAGGTT CCTCCGCGGA GCAGGCTGCC GACTACAGGT CCATTCTGAG CATTAGTGAC 
GAGGCAGCCA GGGTGCAGGC CCTGGACCAA CACCTCAGCA CGCGTAGCTA TATCCAGGGG 
TACTCCCTAT CCCAGGCAGA TGTGGACGTG TTCAGGCAGC TCTCGGCCCC TCCCGCTGAC 
TCAAGGCTCT TCCACGTGGC TCGGTGGTTC AGGCACATAG AAGCGCTCCT GGGTGGCCCC 
CAAGGCAGAG ACGAGCCCTG CAGGCTTCAA GCAAGTAAAG GCCGGCGAGT GCAGCCTCAG 
TGGTCCCCCC CAGCAGGGAC TGAGCCCTGC AGGCTCCGCC TCTATAATAG CCTCACCCGG 
AACAAGGATG TATTTATACC TCAAGATGGG AAGAAGGTGA CGTGGTACTG TTGCGGGCCG 
ACTGTCTATG ATGCGTCTCA CATGGGACAT GCCAGGTCCT ACATCTCCTT CGATATCCTG 
AGGAGGGTGC TGAGGGATTA CTTCCAGTAT GATGTCTTTT ACTGCATGAA CATCACAGAC 
ATTGATGATA AGATCATCAG GCGGGCACGG CAGAACTACC TGTTTGAGCA GTATCGGGAG 
CAGAAACCCC CAGCCACCCA GCTCCTGAAG GATGTCCGTG ATGCCATGAA GCCATTTTCA 
GTCAAGTTAA GTGAGACAAC AGATCCCGAC AAGAGGCAGA TGCTGGAGCG GATCCAGAAC 
TCTGTGAAAC TTGCCACAGA ACCACTGGAA CAGGCTGTGC GATCCAGCCT CTCTGGAGAG 
GAAGTAGACA GCAAAGTACA GGTGTTGCTG GAGGAAGCAA AGGACTTGCT CTCTGACTGG 
CTGGATTCCA CAGGTGGCAG TGAGGTGACT GACAACTCGA TTTTCTCCAA ACTGCCCAAG 
TTCTGGGAAG AAGAGTTCCA CAAAGACATG GAAGCCCTGA ATGTTCTCCC TCCTGATGTC 
CTAACCCGTG TCAGTGAATA TGTGCCAGAA ATTGTGAACT TCGTCCAGAA GATTGTGGAC 
AATGGTTATG GTTATGCTTC AAATGGATCT GTTTACTTCG ACACAGCCAA GTTTGCTGCT 
AGTGAAAAGC ACTCCTATGG GAAGCTGGTG CCTGAGGCCG TTGGGGATCA GAAGGCACTT 
CAGGAAGGGG AAGGTGATCT GAGCATCTCT GCTGACCGCC TTAGTGAGAA ACGCTCTCCC 
AATGACTTTG CCTTGTGGAA GGCCTCCAAG CCAGGCGAGC CATCCTGGCC TTGCCCCTGG 
GGAAAGGGTC GTCCCGGATG GCACATAGAG TGTTCTGCTA TGGCAGGCAC GCTCCTGGGA 
GCCTCAATGG ACATTCATGG TGGAGGGTTT GACCTCCGCT TCCCCCACCA TGACAATGAG 
CTGGCACAGT CGGAGGCCTA CTTTGAAAAT GACTGCTGGG TCAGGTACTT CTTGCACACG 
GGCCACTTGA CGATAGCAGG CTGCAAGATG TCCAAGTCAC TGAAAAACTT TATCACCATT 
AAAGATGCCT TGAAGAAGCA CTCAGCACGG CAGCTGCGAC TGGCATTCCT CATGCACTCA 
TGGAAAGACA CACTGGACTA TTCCAGCAAC ACTATGGAGT CTGCTCTTCA GTATGAAAAA 
TTCATGAACG AGTTTTTCTT AAATGTGAAA GACATCCTCC GAGCCCCTGT AGACATCACT 
GGCCAGTTTG AAAAGTGGGA AGCTGAAGAA GTGGAGCTAA ATAAGAACTT CTATGGCAAG 
AAGACCGCAG TTCACGAAGC CTTGTGTGAT AACATTGACA CCCGGACTGT CATGGAAGAG 
ATGCGGGCTT TGGTCAGTCA GTGCAACCTC TACATGGCAG CCAGGAAGGC TGAACGGAGG 
AGGCCCAACC GGGCTCTGCT GGAGAACATT GCCATGTACC TCACCCACAT GCTGAAGATC 
TTTGGGGCCA TAGAGGAGGA GAGCCCCCTG GGGTTCCCAG TTGGTGGGCC TGGAACCAAC 
CTGAACCTCG AGTCAACAGT CATGCCCTAC CTTCAGGTGT TATCAGAATT CAGAGAGGGA 
GTACGAAAGA TTGCCCGAGA GAAAAAAAGT TCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A Dynamic Unfolded Protein Response Contributes to the Control of Cortical Neurogenesis.
Developmental cell35(5)553-67(2015 Dec)
Laguesse S,Creppe C,Nedialkova DD,Pr?vot PP,Borgs L,Huysseune S,Franco B,Duysens G,Krusy N,Lee G,Thelen N,Thiry M,Close P,Chariot A,Malgrange B,Leidel SA,Godin JD,Nguyen L

Kcnq1ot1 noncoding RNA mediates transcriptional gene silencing by interacting with Dnmt1.
Development (Cambridge, England)137(15)2493-9(2010 Aug)
Mohammad F,Mondal T,Guseva N,Pandey GK,Kanduri C

A germline-specific class of small RNAs binds mammalian Piwi proteins.
Nature442(7099)199-202(2006 Jul)
Girard A,Sachidanandam R,Hannon GJ,Carmell MA

Identification of a new murine tumor necrosis factor receptor locus that contains two novel murine receptors for tumor necrosis factor-related apoptosis-inducing ligand (TRAIL).
The Journal of biological chemistry278(7)5444-54(2003 Feb)
Schneider P,Olson D,Tardivel A,Browning B,Lugovskoy A,Gong D,Dobles M,Hertig S,Hofmann K,Van Vlijmen H,Hsu YM,Burkly LC,Tschopp J,Zheng TS

Gene density and transcription influence the localization of chromatin outside of chromosome territories detectable by FISH.
The Journal of cell biology159(5)753-63(2002 Dec)
Mahy NL,Perry PE,Bickmore WA

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Cars cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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