Esyt3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Esyt3
Entrez Gene ID	272636
Full Name	extended synaptotagmin-like protein 3
Synonyms	D930024E11,D9Ertd280e,Fam62c,mKIAA4186
General protein information	Preferred Names extended synaptotagmin-like protein 3 Names extended synaptotagmin-3 E-Syt3 family with sequence similarity 62 (C2 domain containing), member C family with sequence similarity 62, member C
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9 E3.3\|9 51.54 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_177775.3	NP_808443.2	extended synaptotagmin-3
XM_006511221.3	XP_006511284.1	extended synaptotagmin-3 isoform X1
XM_006511221.4	XP_006511284.1	extended synaptotagmin-3 isoform X1
XM_011242752.2	XP_011241054.1	extended synaptotagmin-3 isoform X2

Pathways from BioSystems

Homologies

	LOC716304	XP_001114238.2
Mus musculus (house mouse)	Esyt3	NP_808443.2
Arabidopsis thaliana (thale cress)	NTMC2T2.1	NP_172041.2
Canis lupus familiaris (dog)	ESYT3	XP_542806.3
Rattus norvegicus (Norway rat)	Esyt3	XP_343455.4
Gallus gallus (chicken)	FAM62C	XP_422118.3
Xenopus tropicalis (tropical clawed frog)	esyt3	NP_001011364.1
Homo sapiens (human)	ESYT3	NP_114119.2
Arabidopsis thaliana (thale cress)	SYTD	NP_196671.2
Oryza sativa (rice)	Os08g0300200	NP_001061492.1
Pan troglodytes (chimpanzee)	ESYT3	XP_003310063.1
Bos taurus (cattle)	ESYT3	XP_002685096.1
Danio rerio (zebrafish)	esyt3	NP_001116705.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Loss of all 3 Extended Synaptotagmins does not affect normal mouse development, viability or fertility.
Tremblay MG, Moss T
Cell cycle (Georgetown, Tex.)15(17)2360-6(2016 Sep)

Extended Synaptotagmin (ESyt) Triple Knock-Out Mice Are Viable and Fertile without Obvious Endoplasmic Reticulum Dysfunction.
Sclip A, Bacaj T, Giam LR, S?dhof TC
PloS one11(6)e0158295(2016)

Loss of Extended Synaptotagmins ESyt2 and ESyt3 does not affect mouse development or viability, but in vitro cell migration and survival under stress are affected.
Herdman C, Tremblay MG, Mishra PK, Moss T
Cell cycle (Georgetown, Tex.)13(16)2616-25(2014)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Esyt3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Esyt3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu74485	XM_011242752.2 Latest version!	Mus musculus extended synaptotagmin-like protein 3 (Esyt3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu20770	NM_177775.3 Latest version!	Mus musculus extended synaptotagmin-like protein 3 (Esyt3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu20770	XM_006511221.4 Latest version!	Mus musculus extended synaptotagmin-like protein 3 (Esyt3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OMu20770	XM_006511221.3	Mus musculus extended synaptotagmin-like protein 3 (Esyt3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Esyt3 ( XM_011242752.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011241054.1 Mus musculus extended synaptotagmin-like protein 3 (Esyt3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu74485
Clone ID Related Accession (Same CDS sequence)	XM_011242752.2
Accession Version	XM_011242752.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1884bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	extended synaptotagmin-3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011242752.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGGGA TCAATGAGTT ATCAGACAGC CTGCTGGAGG ACCTCATTGC TGCCCACCTG 
GTGCTGCCCA ACCGTGTGAC TGTGCCTGTG AAGAAGGGGC TCGATGTAAC CAACCTGCGC 
GTCCCTCTCC CCTGTGGTGT GATCCGAGTC CATTTGCTGG AGGCCAAGAA GCTGGCCCAG 
AAGGACAACT TCCTAGGGCT CGGAGGCAAG TCAGACCCCT ACGCCAAGGT GAGCATTGGC 
TTGCAGCATT GCCGGAGCAG AACCATCTAC AAGAACCTGA ACCCCACCTG GAATGAAGTA 
TTTGAGTTTA TGGTGTACGA GGTACCTGGG CAGGACCTGG AGGTGGACCT GTATGATGAG 
GATACTGACA AGGATGACTT CCTGGGCAGC CTGCAGATCT GCCTTGGAGA TGTCATGAAG 
AACAGAGTGG TGGATGAGTG GTTTGCCCTG AATGACACGA CCAGTGGGCG GCTGCACCTG 
CGGCTGGAGT GGCTCTCACT ACTCACTGAC CAGGAAGCGC TGACGGAGAA TGATAGTGGC 
CTCTCCACTG CCATCCTCGT GGTCTTCTTA GAGAATGCCT GCAACCTGCC GAGAAACCCT 
TTTGACTATC TGAATGGTGA ATATCGAGCC AAAAAACTCT CCAGGTTTGT CAAAAATAAG 
GCCAGCAGAG ACCCTTCTTC ATACGTCAAG CTCACTGTAG GCAAGAAGAC CTTTACAAGT 
AAGACTTGTC CCCACAGCAA GGACCCTGTG TGGAGCCAGG TGTTCTCCTT CTTTGTGCAC 
AGCGTGGCAG CCGAGCAGCT CTGCCTGAAG GTGCTGGACG ATGAGCTAGA GTGTGCTCTG 
GGAGTGCTGG AGTTCCCCCT GTGCCGGATC CTCCCCTGTG CTGACCTCAC CCTCGAACAG 
TGCTTCCAGC TGGATCACTC AGGCCTGGAC AGCCTCATCT CCATGAGGCT GGTGCTTCGG 
TTCCTACGTG TGGAGGGACG AGAGCTGGGA AGCCCATACA CTGGACCCGA CGCTCTAAAG 
AAAGGTCCTC TGTTCATCAA GAAGGTGGCC ACCAACCAGG GTTGTAAAGC CCCACCCCTA 
AACGAAGGCC TTGCAGATGT GACAAGCACC TCGAACCCTG CCTCTTACAT CAAAGGAGCC 
TCCAAGAGCA TTGACAATAT CAGCGCAGCC ACCACAGACC CTGAACCTAT GCCTGAGCCC 
CAGGGGCCAG GCCCGGAGCC CAAAGGCAAG GACAGTGCCA GAGGGCTCTG TGAGTCCCCT 
GGAAAGAAGA AGAACCCAGC TACCACCTTC CTGACTGTCC CAGGTCTCCA CTCTCCAGGG 
CCCATCAAGT CACCCAGACC CATGAGCCGC CCTGCCTTCC CATTCGCATG GCCACTCACG 
AGGGTGGCTC CCAGCATGTC CTCACTCAAC TCCCTGGCCT CTTCCTGCTT TGACCTGACA 
GATGTCAGCC TCAACACTGA AGCTGGGGAT TCCAGGCAGG GACGGCTGGG TGAGATTCAG 
CTCACAGTGC GCTATGTGTG TCTCCGACAC TGTCTCAGAG TGCTGGTCAA CGGCTGCAGA 
AACTTAACAC CATGCACCAG CAGTGGAGCT GACCCCTACG TCCGCATCTA CCTGCTGCCA 
GAGAGAAGAT GGGCGAGTCG GAAGAAGACC TCAGTAAAAC AGAAGACCCT GGAGCCCCTA 
TTCGATGAGA CGTTTGAGTT TTTTGTCCCC ATGGGAGAAG TGCAGAAGAG ATCACTGGAT 
GTCGCAGTGA AAAACAGCAG GCCTCTCGGC TCACACAGAA GGAAGGAGTT GGGGAAAGTG 
CTGATTGACT TATCAAAGCA AGATCTGATT AAGGGCTTCT CACAGTGGTA TGAACTGACT 
GCAGATGGAC AGCCCAGAAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011241054.1
CDS	190..2073
Translation	MPGINELSDSLLEDLIAAHLVLPNRVTVPVKKGLDVTNLRVPLPCGVIRVHLLEAKKLAQKDNFLGLGGKSDPYAKVSIGLQHCRSRTIYKNLNPTWNEVFEFMVYEVPGQDLEVDLYDEDTDKDDFLGSLQICLGDVMKNRVVDEWFALNDTTSGRLHLRLEWLSLLTDQEALTENDSGLSTAILVVFLENACNLPRNPFDYLNGEYRAKKLSRFVKNKASRDPSSYVKLTVGKKTFTSKTCPHSKDPVWSQVFSFFVHSVAAEQLCLKVLDDELECALGVLEFPLCRILPCADLTLEQCFQLDHSGLDSLISMRLVLRFLRVEGRELGSPYTGPDALKKGPLFIKKVATNQGCKAPPLNEGLADVTSTSNPASYIKGASKSIDNISAATTDPEPMPEPQGPGPEPKGKDSARGLCESPGKKKNPATTFLTVPGLHSPGPIKSPRPMSRPAFPFAWPLTRVAPSMSSLNSLASSCFDLTDVSLNTEAGDSRQGRLGEIQLTVRYVCLRHCLRVLVNGCRNLTPCTSSGADPYVRIYLLPERRWASRKKTSVKQKTLEPLFDETFEFFVPMGEVQKRSLDVAVKNSRPLGSHRRKELGKVLIDLSKQDLIKGFSQWYELTADGQPRS

Target ORF information:

RefSeq Version	XM_011242752.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus extended synaptotagmin-like protein 3 (Esyt3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011242752.2

ORF Insert Sequence:

ATGCCGGGGA TCAATGAGTT ATCAGACAGC CTGCTGGAGG ACCTCATTGC TGCCCACCTG 
GTGCTGCCCA ACCGTGTGAC TGTGCCTGTG AAGAAGGGGC TCGATGTAAC CAACCTGCGC 
GTCCCTCTCC CCTGTGGTGT GATCCGAGTC CATTTGCTGG AGGCCAAGAA GCTGGCCCAG 
AAGGACAACT TCCTAGGGCT CGGAGGCAAG TCAGACCCCT ACGCCAAGGT GAGCATTGGC 
TTGCAGCATT GCCGGAGCAG AACCATCTAC AAGAACCTGA ACCCCACCTG GAATGAAGTA 
TTTGAGTTTA TGGTGTACGA GGTACCTGGG CAGGACCTGG AGGTGGACCT GTATGATGAG 
GATACTGACA AGGATGACTT CCTGGGCAGC CTGCAGATCT GCCTTGGAGA TGTCATGAAG 
AACAGAGTGG TGGATGAGTG GTTTGCCCTG AATGACACGA CCAGTGGGCG GCTGCACCTG 
CGGCTGGAGT GGCTCTCACT ACTCACTGAC CAGGAAGCGC TGACGGAGAA TGATAGTGGC 
CTCTCCACTG CCATCCTCGT GGTCTTCTTA GAGAATGCCT GCAACCTGCC GAGAAACCCT 
TTTGACTATC TGAATGGTGA ATATCGAGCC AAAAAACTCT CCAGGTTTGT CAAAAATAAG 
GCCAGCAGAG ACCCTTCTTC ATACGTCAAG CTCACTGTAG GCAAGAAGAC CTTTACAAGT 
AAGACTTGTC CCCACAGCAA GGACCCTGTG TGGAGCCAGG TGTTCTCCTT CTTTGTGCAC 
AGCGTGGCAG CCGAGCAGCT CTGCCTGAAG GTGCTGGACG ATGAGCTAGA GTGTGCTCTG 
GGAGTGCTGG AGTTCCCCCT GTGCCGGATC CTCCCCTGTG CTGACCTCAC CCTCGAACAG 
TGCTTCCAGC TGGATCACTC AGGCCTGGAC AGCCTCATCT CCATGAGGCT GGTGCTTCGG 
TTCCTACGTG TGGAGGGACG AGAGCTGGGA AGCCCATACA CTGGACCCGA CGCTCTAAAG 
AAAGGTCCTC TGTTCATCAA GAAGGTGGCC ACCAACCAGG GTTGTAAAGC CCCACCCCTA 
AACGAAGGCC TTGCAGATGT GACAAGCACC TCGAACCCTG CCTCTTACAT CAAAGGAGCC 
TCCAAGAGCA TTGACAATAT CAGCGCAGCC ACCACAGACC CTGAACCTAT GCCTGAGCCC 
CAGGGGCCAG GCCCGGAGCC CAAAGGCAAG GACAGTGCCA GAGGGCTCTG TGAGTCCCCT 
GGAAAGAAGA AGAACCCAGC TACCACCTTC CTGACTGTCC CAGGTCTCCA CTCTCCAGGG 
CCCATCAAGT CACCCAGACC CATGAGCCGC CCTGCCTTCC CATTCGCATG GCCACTCACG 
AGGGTGGCTC CCAGCATGTC CTCACTCAAC TCCCTGGCCT CTTCCTGCTT TGACCTGACA 
GATGTCAGCC TCAACACTGA AGCTGGGGAT TCCAGGCAGG GACGGCTGGG TGAGATTCAG 
CTCACAGTGC GCTATGTGTG TCTCCGACAC TGTCTCAGAG TGCTGGTCAA CGGCTGCAGA 
AACTTAACAC CATGCACCAG CAGTGGAGCT GACCCCTACG TCCGCATCTA CCTGCTGCCA 
GAGAGAAGAT GGGCGAGTCG GAAGAAGACC TCAGTAAAAC AGAAGACCCT GGAGCCCCTA 
TTCGATGAGA CGTTTGAGTT TTTTGTCCCC ATGGGAGAAG TGCAGAAGAG ATCACTGGAT 
GTCGCAGTGA AAAACAGCAG GCCTCTCGGC TCACACAGAA GGAAGGAGTT GGGGAAAGTG 
CTGATTGACT TATCAAAGCA AGATCTGATT AAGGGCTTCT CACAGTGGTA TGAACTGACT 
GCAGATGGAC AGCCCAGAAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Esyt3 ( NM_177775.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_808443.2 Mus musculus extended synaptotagmin-like protein 3 (Esyt3), mRNA.

Price & Availability↑

CloneID	OMu20770
Clone ID Related Accession (Same CDS sequence)	NM_177775.3 , XM_006511221.3 , XM_006511221.4
Accession Version	NM_177775.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2676bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-19
Organism	Mus musculus(house mouse)
Product	extended synaptotagmin-3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK086367.1, AK142948.1 and BY754935.1. On Dec 15, 2006 this sequence version replaced NM_177775.2. ##Evidence-Data-START## Transcript exon combination :: AK220532.1, AK086367.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGCAGCCGG AGGAGCCCTG CGCCCCGAGC GCGCCCGGCG GTCCTGACGT CCCGGAGAGA 
GGCCAGCGCT CGCGGGACCC CGGACCGCGC CTGTCGGGCC AGCTACTGCC CGAGCTCTAC 
AGCTTCGTGG CGCGCGTCCT TTTCTACCTG GCACCGGTCT ACCTGGCGGG CTACCTGGGG 
CTCAGCGTCA CCTGGCTGCT GCTTGGCGCC CTGCTCTGGA TGTGGTGGCG CCGGAACCGC 
CGCGGGAAGC TGGGGCGCTT GGAGGCCGCC TTCGAATTCC TGGAGCACGA GCGCGAGTTC 
ATCAGCCGTG AGCTGCGGGG CCAGCATTTG CCAGCCTGGA TACATTTCCC AGATGTGGAG 
CGTGTGGAGT GGGCCAATAA GATCATCATA CAGATCTGGC CCTACCTAAG CATGATCATG 
GAGAACAAGA TCCGGGAGAA GCTGGAGCCC AAGATCCGGG AGAAGAGCAT CCACCTCAGA 
ACCTTCACCT TCACCAAGCT CTACTTTGGG CAGAAGTGTC CCAAGGTGAA CGGCGTCAAG 
GTACACACGG ACAAACGCAA CCGGAGGAAA GTGACCCTGG ACCTGCAGAT CTGCTACATC 
GGGGACTGTG AGATCAGCGT GGAACTTCAG AAGATCCGGG GCGGTGTGAG CGGCGTCCAG 
TTGCAGGGGA CCCTGCGTGT CATTCTGGAG CCCCTGCTGG TGGACAAGCC CTTCATAGGA 
GCTGTGACAG TGTTCTTCCT TCAAAAGCCG CACCTGCAGA TTAACTGGAC AGGCTTGACC 
AACCTGCTGG ACATGCCGGG GATCAATGAG TTATCAGACA GCCTGCTGGA GGACCTCATT 
GCTGCCCACC TGGTGCTGCC CAACCGTGTG ACTGTGCCTG TGAAGAAGGG GCTCGATGTA 
ACCAACCTGC GCGTCCCTCT CCCCTGTGGT GTGATCCGAG TCCATTTGCT GGAGGCCAAG 
AAGCTGGCCC AGAAGGACAA CTTCCTAGGG CTCGGAGGCA AGTCAGACCC CTACGCCAAG 
GTGAGCATTG GCTTGCAGCA TTGCCGGAGC AGAACCATCT ACAAGAACCT GAACCCCACC 
TGGAATGAAG TATTTGAGTT TATGGTGTAC GAGGTACCTG GGCAGGACCT GGAGGTGGAC 
CTGTATGATG AGGATACTGA CAAGGATGAC TTCCTGGGCA GCCTGCAGAT CTGCCTTGGA 
GATGTCATGA AGAACAGAGT GGTGGATGAG TGGTTTGCCC TGAATGACAC GACCAGTGGG 
CGGCTGCACC TGCGGCTGGA GTGGCTCTCA CTACTCACTG ACCAGGAAGC GCTGACGGAG 
AATGATAGTG GCCTCTCCAC TGCCATCCTC GTGGTCTTCT TAGAGAATGC CTGCAACCTG 
CCGAGAAACC CTTTTGACTA TCTGAATGGT GAATATCGAG CCAAAAAACT CTCCAGGTTT 
GTCAAAAATA AGGCCAGCAG AGACCCTTCT TCATACGTCA AGCTCACTGT AGGCAAGAAG 
ACCTTTACAA GTAAGACTTG TCCCCACAGC AAGGACCCTG TGTGGAGCCA GGTGTTCTCC 
TTCTTTGTGC ACAGCGTGGC AGCCGAGCAG CTCTGCCTGA AGGTGCTGGA CGATGAGCTA 
GAGTGTGCTC TGGGAGTGCT GGAGTTCCCC CTGTGCCGGA TCCTCCCCTG TGCTGACCTC 
ACCCTCGAAC AGTGCTTCCA GCTGGATCAC TCAGGCCTGG ACAGCCTCAT CTCCATGAGG 
CTGGTGCTTC GGTTCCTACG TGTGGAGGGA CGAGAGCTGG GAAGCCCATA CACTGGACCC 
GACGCTCTAA AGAAAGGTCC TCTGTTCATC AAGAAGGTGG CCACCAACCA GGGTTGTAAA 
GCCCCACCCC TAAACGAAGG CCTTGCAGAT GTGACAAGCA CCTCGAACCC TGCCTCTTAC 
ATCAAAGGAG CCTCCAAGAG CATTGACAAT ATCAGCGCAG CCACCACAGA CCCTGAACCT 
ATGCCTGAGC CCCAGGGGCC AGGCCCGGAG CCCAAAGGCA AGGACAGTGC CAGAGGGCTC 
TGTGAGTCCC CTGGAAAGAA GAAGAACCCA GCTACCACCT TCCTGACTGT CCCAGGTCTC 
CACTCTCCAG GGCCCATCAA GTCACCCAGA CCCATGAGCC GCCCTGCCTT CCCATTCGCA 
TGGCCACTCA CGAGGGTGGC TCCCAGCATG TCCTCACTCA ACTCCCTGGC CTCTTCCTGC 
TTTGACCTGA CAGATGTCAG CCTCAACACT GAAGCTGGGG ATTCCAGGCA GGGACGGCTG 
GGTGAGATTC AGCTCACAGT GCGCTATGTG TGTCTCCGAC ACTGTCTCAG AGTGCTGGTC 
AACGGCTGCA GAAACTTAAC ACCATGCACC AGCAGTGGAG CTGACCCCTA CGTCCGCATC 
TACCTGCTGC CAGAGAGAAG ATGGGCGAGT CGGAAGAAGA CCTCAGTAAA ACAGAAGACC 
CTGGAGCCCC TATTCGATGA GACGTTTGAG TTTTTTGTCC CCATGGGAGA AGTGCAGAAG 
AGATCACTGG ATGTCGCAGT GAAAAACAGC AGGCCTCTCG GCTCACACAG AAGGAAGGAG 
TTGGGGAAAG TGCTGATTGA CTTATCAAAG CAAGATCTGA TTAAGGGCTT CTCACAGTGG 
TATGAACTGA CTGCAGATGG ACAGCCCAGA AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_808443.2
CDS	142..2817
Translation	MQPEEPCAPSAPGGPDVPERGQRSRDPGPRLSGQLLPELYSFVARVLFYLAPVYLAGYLGLSVTWLLLGALLWMWWRRNRRGKLGRLEAAFEFLEHEREFISRELRGQHLPAWIHFPDVERVEWANKIIIQIWPYLSMIMENKIREKLEPKIREKSIHLRTFTFTKLYFGQKCPKVNGVKVHTDKRNRRKVTLDLQICYIGDCEISVELQKIRGGVSGVQLQGTLRVILEPLLVDKPFIGAVTVFFLQKPHLQINWTGLTNLLDMPGINELSDSLLEDLIAAHLVLPNRVTVPVKKGLDVTNLRVPLPCGVIRVHLLEAKKLAQKDNFLGLGGKSDPYAKVSIGLQHCRSRTIYKNLNPTWNEVFEFMVYEVPGQDLEVDLYDEDTDKDDFLGSLQICLGDVMKNRVVDEWFALNDTTSGRLHLRLEWLSLLTDQEALTENDSGLSTAILVVFLENACNLPRNPFDYLNGEYRAKKLSRFVKNKASRDPSSYVKLTVGKKTFTSKTCPHSKDPVWSQVFSFFVHSVAAEQLCLKVLDDELECALGVLEFPLCRILPCADLTLEQCFQLDHSGLDSLISMRLVLRFLRVEGRELGSPYTGPDALKKGPLFIKKVATNQGCKAPPLNEGLADVTSTSNPASYIKGASKSIDNISAATTDPEPMPEPQGPGPEPKGKDSARGLCESPGKKKNPATTFLTVPGLHSPGPIKSPRPMSRPAFPFAWPLTRVAPSMSSLNSLASSCFDLTDVSLNTEAGDSRQGRLGEIQLTVRYVCLRHCLRVLVNGCRNLTPCTSSGADPYVRIYLLPERRWASRKKTSVKQKTLEPLFDETFEFFVPMGEVQKRSLDVAVKNSRPLGSHRRKELGKVLIDLSKQDLIKGFSQWYELTADGQPRS

Target ORF information:

RefSeq Version	NM_177775.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus extended synaptotagmin-like protein 3 (Esyt3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177775.3

ORF Insert Sequence:

ATGCAGCCGG AGGAGCCCTG CGCCCCGAGC GCGCCCGGCG GTCCTGACGT CCCGGAGAGA 
GGCCAGCGCT CGCGGGACCC CGGACCGCGC CTGTCGGGCC AGCTACTGCC CGAGCTCTAC 
AGCTTCGTGG CGCGCGTCCT TTTCTACCTG GCACCGGTCT ACCTGGCGGG CTACCTGGGG 
CTCAGCGTCA CCTGGCTGCT GCTTGGCGCC CTGCTCTGGA TGTGGTGGCG CCGGAACCGC 
CGCGGGAAGC TGGGGCGCTT GGAGGCCGCC TTCGAATTCC TGGAGCACGA GCGCGAGTTC 
ATCAGCCGTG AGCTGCGGGG CCAGCATTTG CCAGCCTGGA TACATTTCCC AGATGTGGAG 
CGTGTGGAGT GGGCCAATAA GATCATCATA CAGATCTGGC CCTACCTAAG CATGATCATG 
GAGAACAAGA TCCGGGAGAA GCTGGAGCCC AAGATCCGGG AGAAGAGCAT CCACCTCAGA 
ACCTTCACCT TCACCAAGCT CTACTTTGGG CAGAAGTGTC CCAAGGTGAA CGGCGTCAAG 
GTACACACGG ACAAACGCAA CCGGAGGAAA GTGACCCTGG ACCTGCAGAT CTGCTACATC 
GGGGACTGTG AGATCAGCGT GGAACTTCAG AAGATCCGGG GCGGTGTGAG CGGCGTCCAG 
TTGCAGGGGA CCCTGCGTGT CATTCTGGAG CCCCTGCTGG TGGACAAGCC CTTCATAGGA 
GCTGTGACAG TGTTCTTCCT TCAAAAGCCG CACCTGCAGA TTAACTGGAC AGGCTTGACC 
AACCTGCTGG ACATGCCGGG GATCAATGAG TTATCAGACA GCCTGCTGGA GGACCTCATT 
GCTGCCCACC TGGTGCTGCC CAACCGTGTG ACTGTGCCTG TGAAGAAGGG GCTCGATGTA 
ACCAACCTGC GCGTCCCTCT CCCCTGTGGT GTGATCCGAG TCCATTTGCT GGAGGCCAAG 
AAGCTGGCCC AGAAGGACAA CTTCCTAGGG CTCGGAGGCA AGTCAGACCC CTACGCCAAG 
GTGAGCATTG GCTTGCAGCA TTGCCGGAGC AGAACCATCT ACAAGAACCT GAACCCCACC 
TGGAATGAAG TATTTGAGTT TATGGTGTAC GAGGTACCTG GGCAGGACCT GGAGGTGGAC 
CTGTATGATG AGGATACTGA CAAGGATGAC TTCCTGGGCA GCCTGCAGAT CTGCCTTGGA 
GATGTCATGA AGAACAGAGT GGTGGATGAG TGGTTTGCCC TGAATGACAC GACCAGTGGG 
CGGCTGCACC TGCGGCTGGA GTGGCTCTCA CTACTCACTG ACCAGGAAGC GCTGACGGAG 
AATGATAGTG GCCTCTCCAC TGCCATCCTC GTGGTCTTCT TAGAGAATGC CTGCAACCTG 
CCGAGAAACC CTTTTGACTA TCTGAATGGT GAATATCGAG CCAAAAAACT CTCCAGGTTT 
GTCAAAAATA AGGCCAGCAG AGACCCTTCT TCATACGTCA AGCTCACTGT AGGCAAGAAG 
ACCTTTACAA GTAAGACTTG TCCCCACAGC AAGGACCCTG TGTGGAGCCA GGTGTTCTCC 
TTCTTTGTGC ACAGCGTGGC AGCCGAGCAG CTCTGCCTGA AGGTGCTGGA CGATGAGCTA 
GAGTGTGCTC TGGGAGTGCT GGAGTTCCCC CTGTGCCGGA TCCTCCCCTG TGCTGACCTC 
ACCCTCGAAC AGTGCTTCCA GCTGGATCAC TCAGGCCTGG ACAGCCTCAT CTCCATGAGG 
CTGGTGCTTC GGTTCCTACG TGTGGAGGGA CGAGAGCTGG GAAGCCCATA CACTGGACCC 
GACGCTCTAA AGAAAGGTCC TCTGTTCATC AAGAAGGTGG CCACCAACCA GGGTTGTAAA 
GCCCCACCCC TAAACGAAGG CCTTGCAGAT GTGACAAGCA CCTCGAACCC TGCCTCTTAC 
ATCAAAGGAG CCTCCAAGAG CATTGACAAT ATCAGCGCAG CCACCACAGA CCCTGAACCT 
ATGCCTGAGC CCCAGGGGCC AGGCCCGGAG CCCAAAGGCA AGGACAGTGC CAGAGGGCTC 
TGTGAGTCCC CTGGAAAGAA GAAGAACCCA GCTACCACCT TCCTGACTGT CCCAGGTCTC 
CACTCTCCAG GGCCCATCAA GTCACCCAGA CCCATGAGCC GCCCTGCCTT CCCATTCGCA 
TGGCCACTCA CGAGGGTGGC TCCCAGCATG TCCTCACTCA ACTCCCTGGC CTCTTCCTGC 
TTTGACCTGA CAGATGTCAG CCTCAACACT GAAGCTGGGG ATTCCAGGCA GGGACGGCTG 
GGTGAGATTC AGCTCACAGT GCGCTATGTG TGTCTCCGAC ACTGTCTCAG AGTGCTGGTC 
AACGGCTGCA GAAACTTAAC ACCATGCACC AGCAGTGGAG CTGACCCCTA CGTCCGCATC 
TACCTGCTGC CAGAGAGAAG ATGGGCGAGT CGGAAGAAGA CCTCAGTAAA ACAGAAGACC 
CTGGAGCCCC TATTCGATGA GACGTTTGAG TTTTTTGTCC CCATGGGAGA AGTGCAGAAG 
AGATCACTGG ATGTCGCAGT GAAAAACAGC AGGCCTCTCG GCTCACACAG AAGGAAGGAG 
TTGGGGAAAG TGCTGATTGA CTTATCAAAG CAAGATCTGA TTAAGGGCTT CTCACAGTGG 
TATGAACTGA CTGCAGATGG ACAGCCCAGA AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Esyt3 ( XM_006511221.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006511284.1 Mus musculus extended synaptotagmin-like protein 3 (Esyt3), transcript variant X1, mRNA.

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CloneID	OMu20770
Clone ID Related Accession (Same CDS sequence)	NM_177775.3 , XM_006511221.3 , XM_006511221.4
Accession Version	XM_006511221.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2676bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	extended synaptotagmin-3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006511221.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCCGG AGGAGCCCTG CGCCCCGAGC GCGCCCGGCG GTCCTGACGT CCCGGAGAGA 
GGCCAGCGCT CGCGGGACCC CGGACCGCGC CTGTCGGGCC AGCTACTGCC CGAGCTCTAC 
AGCTTCGTGG CGCGCGTCCT TTTCTACCTG GCACCGGTCT ACCTGGCGGG CTACCTGGGG 
CTCAGCGTCA CCTGGCTGCT GCTTGGCGCC CTGCTCTGGA TGTGGTGGCG CCGGAACCGC 
CGCGGGAAGC TGGGGCGCTT GGAGGCCGCC TTCGAATTCC TGGAGCACGA GCGCGAGTTC 
ATCAGCCGTG AGCTGCGGGG CCAGCATTTG CCAGCCTGGA TACATTTCCC AGATGTGGAG 
CGTGTGGAGT GGGCCAATAA GATCATCATA CAGATCTGGC CCTACCTAAG CATGATCATG 
GAGAACAAGA TCCGGGAGAA GCTGGAGCCC AAGATCCGGG AGAAGAGCAT CCACCTCAGA 
ACCTTCACCT TCACCAAGCT CTACTTTGGG CAGAAGTGTC CCAAGGTGAA CGGCGTCAAG 
GTACACACGG ACAAACGCAA CCGGAGGAAA GTGACCCTGG ACCTGCAGAT CTGCTACATC 
GGGGACTGTG AGATCAGCGT GGAACTTCAG AAGATCCGGG GCGGTGTGAG CGGCGTCCAG 
TTGCAGGGGA CCCTGCGTGT CATTCTGGAG CCCCTGCTGG TGGACAAGCC CTTCATAGGA 
GCTGTGACAG TGTTCTTCCT TCAAAAGCCG CACCTGCAGA TTAACTGGAC AGGCTTGACC 
AACCTGCTGG ACATGCCGGG GATCAATGAG TTATCAGACA GCCTGCTGGA GGACCTCATT 
GCTGCCCACC TGGTGCTGCC CAACCGTGTG ACTGTGCCTG TGAAGAAGGG GCTCGATGTA 
ACCAACCTGC GCGTCCCTCT CCCCTGTGGT GTGATCCGAG TCCATTTGCT GGAGGCCAAG 
AAGCTGGCCC AGAAGGACAA CTTCCTAGGG CTCGGAGGCA AGTCAGACCC CTACGCCAAG 
GTGAGCATTG GCTTGCAGCA TTGCCGGAGC AGAACCATCT ACAAGAACCT GAACCCCACC 
TGGAATGAAG TATTTGAGTT TATGGTGTAC GAGGTACCTG GGCAGGACCT GGAGGTGGAC 
CTGTATGATG AGGATACTGA CAAGGATGAC TTCCTGGGCA GCCTGCAGAT CTGCCTTGGA 
GATGTCATGA AGAACAGAGT GGTGGATGAG TGGTTTGCCC TGAATGACAC GACCAGTGGG 
CGGCTGCACC TGCGGCTGGA GTGGCTCTCA CTACTCACTG ACCAGGAAGC GCTGACGGAG 
AATGATAGTG GCCTCTCCAC TGCCATCCTC GTGGTCTTCT TAGAGAATGC CTGCAACCTG 
CCGAGAAACC CTTTTGACTA TCTGAATGGT GAATATCGAG CCAAAAAACT CTCCAGGTTT 
GTCAAAAATA AGGCCAGCAG AGACCCTTCT TCATACGTCA AGCTCACTGT AGGCAAGAAG 
ACCTTTACAA GTAAGACTTG TCCCCACAGC AAGGACCCTG TGTGGAGCCA GGTGTTCTCC 
TTCTTTGTGC ACAGCGTGGC AGCCGAGCAG CTCTGCCTGA AGGTGCTGGA CGATGAGCTA 
GAGTGTGCTC TGGGAGTGCT GGAGTTCCCC CTGTGCCGGA TCCTCCCCTG TGCTGACCTC 
ACCCTCGAAC AGTGCTTCCA GCTGGATCAC TCAGGCCTGG ACAGCCTCAT CTCCATGAGG 
CTGGTGCTTC GGTTCCTACG TGTGGAGGGA CGAGAGCTGG GAAGCCCATA CACTGGACCC 
GACGCTCTAA AGAAAGGTCC TCTGTTCATC AAGAAGGTGG CCACCAACCA GGGTTGTAAA 
GCCCCACCCC TAAACGAAGG CCTTGCAGAT GTGACAAGCA CCTCGAACCC TGCCTCTTAC 
ATCAAAGGAG CCTCCAAGAG CATTGACAAT ATCAGCGCAG CCACCACAGA CCCTGAACCT 
ATGCCTGAGC CCCAGGGGCC AGGCCCGGAG CCCAAAGGCA AGGACAGTGC CAGAGGGCTC 
TGTGAGTCCC CTGGAAAGAA GAAGAACCCA GCTACCACCT TCCTGACTGT CCCAGGTCTC 
CACTCTCCAG GGCCCATCAA GTCACCCAGA CCCATGAGCC GCCCTGCCTT CCCATTCGCA 
TGGCCACTCA CGAGGGTGGC TCCCAGCATG TCCTCACTCA ACTCCCTGGC CTCTTCCTGC 
TTTGACCTGA CAGATGTCAG CCTCAACACT GAAGCTGGGG ATTCCAGGCA GGGACGGCTG 
GGTGAGATTC AGCTCACAGT GCGCTATGTG TGTCTCCGAC ACTGTCTCAG AGTGCTGGTC 
AACGGCTGCA GAAACTTAAC ACCATGCACC AGCAGTGGAG CTGACCCCTA CGTCCGCATC 
TACCTGCTGC CAGAGAGAAG ATGGGCGAGT CGGAAGAAGA CCTCAGTAAA ACAGAAGACC 
CTGGAGCCCC TATTCGATGA GACGTTTGAG TTTTTTGTCC CCATGGGAGA AGTGCAGAAG 
AGATCACTGG ATGTCGCAGT GAAAAACAGC AGGCCTCTCG GCTCACACAG AAGGAAGGAG 
TTGGGGAAAG TGCTGATTGA CTTATCAAAG CAAGATCTGA TTAAGGGCTT CTCACAGTGG 
TATGAACTGA CTGCAGATGG ACAGCCCAGA AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006511284.1
CDS	1091..3766
Translation	MQPEEPCAPSAPGGPDVPERGQRSRDPGPRLSGQLLPELYSFVARVLFYLAPVYLAGYLGLSVTWLLLGALLWMWWRRNRRGKLGRLEAAFEFLEHEREFISRELRGQHLPAWIHFPDVERVEWANKIIIQIWPYLSMIMENKIREKLEPKIREKSIHLRTFTFTKLYFGQKCPKVNGVKVHTDKRNRRKVTLDLQICYIGDCEISVELQKIRGGVSGVQLQGTLRVILEPLLVDKPFIGAVTVFFLQKPHLQINWTGLTNLLDMPGINELSDSLLEDLIAAHLVLPNRVTVPVKKGLDVTNLRVPLPCGVIRVHLLEAKKLAQKDNFLGLGGKSDPYAKVSIGLQHCRSRTIYKNLNPTWNEVFEFMVYEVPGQDLEVDLYDEDTDKDDFLGSLQICLGDVMKNRVVDEWFALNDTTSGRLHLRLEWLSLLTDQEALTENDSGLSTAILVVFLENACNLPRNPFDYLNGEYRAKKLSRFVKNKASRDPSSYVKLTVGKKTFTSKTCPHSKDPVWSQVFSFFVHSVAAEQLCLKVLDDELECALGVLEFPLCRILPCADLTLEQCFQLDHSGLDSLISMRLVLRFLRVEGRELGSPYTGPDALKKGPLFIKKVATNQGCKAPPLNEGLADVTSTSNPASYIKGASKSIDNISAATTDPEPMPEPQGPGPEPKGKDSARGLCESPGKKKNPATTFLTVPGLHSPGPIKSPRPMSRPAFPFAWPLTRVAPSMSSLNSLASSCFDLTDVSLNTEAGDSRQGRLGEIQLTVRYVCLRHCLRVLVNGCRNLTPCTSSGADPYVRIYLLPERRWASRKKTSVKQKTLEPLFDETFEFFVPMGEVQKRSLDVAVKNSRPLGSHRRKELGKVLIDLSKQDLIKGFSQWYELTADGQPRS

Target ORF information:

RefSeq Version	XM_006511221.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus extended synaptotagmin-like protein 3 (Esyt3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006511221.3

ORF Insert Sequence:

ATGCAGCCGG AGGAGCCCTG CGCCCCGAGC GCGCCCGGCG GTCCTGACGT CCCGGAGAGA 
GGCCAGCGCT CGCGGGACCC CGGACCGCGC CTGTCGGGCC AGCTACTGCC CGAGCTCTAC 
AGCTTCGTGG CGCGCGTCCT TTTCTACCTG GCACCGGTCT ACCTGGCGGG CTACCTGGGG 
CTCAGCGTCA CCTGGCTGCT GCTTGGCGCC CTGCTCTGGA TGTGGTGGCG CCGGAACCGC 
CGCGGGAAGC TGGGGCGCTT GGAGGCCGCC TTCGAATTCC TGGAGCACGA GCGCGAGTTC 
ATCAGCCGTG AGCTGCGGGG CCAGCATTTG CCAGCCTGGA TACATTTCCC AGATGTGGAG 
CGTGTGGAGT GGGCCAATAA GATCATCATA CAGATCTGGC CCTACCTAAG CATGATCATG 
GAGAACAAGA TCCGGGAGAA GCTGGAGCCC AAGATCCGGG AGAAGAGCAT CCACCTCAGA 
ACCTTCACCT TCACCAAGCT CTACTTTGGG CAGAAGTGTC CCAAGGTGAA CGGCGTCAAG 
GTACACACGG ACAAACGCAA CCGGAGGAAA GTGACCCTGG ACCTGCAGAT CTGCTACATC 
GGGGACTGTG AGATCAGCGT GGAACTTCAG AAGATCCGGG GCGGTGTGAG CGGCGTCCAG 
TTGCAGGGGA CCCTGCGTGT CATTCTGGAG CCCCTGCTGG TGGACAAGCC CTTCATAGGA 
GCTGTGACAG TGTTCTTCCT TCAAAAGCCG CACCTGCAGA TTAACTGGAC AGGCTTGACC 
AACCTGCTGG ACATGCCGGG GATCAATGAG TTATCAGACA GCCTGCTGGA GGACCTCATT 
GCTGCCCACC TGGTGCTGCC CAACCGTGTG ACTGTGCCTG TGAAGAAGGG GCTCGATGTA 
ACCAACCTGC GCGTCCCTCT CCCCTGTGGT GTGATCCGAG TCCATTTGCT GGAGGCCAAG 
AAGCTGGCCC AGAAGGACAA CTTCCTAGGG CTCGGAGGCA AGTCAGACCC CTACGCCAAG 
GTGAGCATTG GCTTGCAGCA TTGCCGGAGC AGAACCATCT ACAAGAACCT GAACCCCACC 
TGGAATGAAG TATTTGAGTT TATGGTGTAC GAGGTACCTG GGCAGGACCT GGAGGTGGAC 
CTGTATGATG AGGATACTGA CAAGGATGAC TTCCTGGGCA GCCTGCAGAT CTGCCTTGGA 
GATGTCATGA AGAACAGAGT GGTGGATGAG TGGTTTGCCC TGAATGACAC GACCAGTGGG 
CGGCTGCACC TGCGGCTGGA GTGGCTCTCA CTACTCACTG ACCAGGAAGC GCTGACGGAG 
AATGATAGTG GCCTCTCCAC TGCCATCCTC GTGGTCTTCT TAGAGAATGC CTGCAACCTG 
CCGAGAAACC CTTTTGACTA TCTGAATGGT GAATATCGAG CCAAAAAACT CTCCAGGTTT 
GTCAAAAATA AGGCCAGCAG AGACCCTTCT TCATACGTCA AGCTCACTGT AGGCAAGAAG 
ACCTTTACAA GTAAGACTTG TCCCCACAGC AAGGACCCTG TGTGGAGCCA GGTGTTCTCC 
TTCTTTGTGC ACAGCGTGGC AGCCGAGCAG CTCTGCCTGA AGGTGCTGGA CGATGAGCTA 
GAGTGTGCTC TGGGAGTGCT GGAGTTCCCC CTGTGCCGGA TCCTCCCCTG TGCTGACCTC 
ACCCTCGAAC AGTGCTTCCA GCTGGATCAC TCAGGCCTGG ACAGCCTCAT CTCCATGAGG 
CTGGTGCTTC GGTTCCTACG TGTGGAGGGA CGAGAGCTGG GAAGCCCATA CACTGGACCC 
GACGCTCTAA AGAAAGGTCC TCTGTTCATC AAGAAGGTGG CCACCAACCA GGGTTGTAAA 
GCCCCACCCC TAAACGAAGG CCTTGCAGAT GTGACAAGCA CCTCGAACCC TGCCTCTTAC 
ATCAAAGGAG CCTCCAAGAG CATTGACAAT ATCAGCGCAG CCACCACAGA CCCTGAACCT 
ATGCCTGAGC CCCAGGGGCC AGGCCCGGAG CCCAAAGGCA AGGACAGTGC CAGAGGGCTC 
TGTGAGTCCC CTGGAAAGAA GAAGAACCCA GCTACCACCT TCCTGACTGT CCCAGGTCTC 
CACTCTCCAG GGCCCATCAA GTCACCCAGA CCCATGAGCC GCCCTGCCTT CCCATTCGCA 
TGGCCACTCA CGAGGGTGGC TCCCAGCATG TCCTCACTCA ACTCCCTGGC CTCTTCCTGC 
TTTGACCTGA CAGATGTCAG CCTCAACACT GAAGCTGGGG ATTCCAGGCA GGGACGGCTG 
GGTGAGATTC AGCTCACAGT GCGCTATGTG TGTCTCCGAC ACTGTCTCAG AGTGCTGGTC 
AACGGCTGCA GAAACTTAAC ACCATGCACC AGCAGTGGAG CTGACCCCTA CGTCCGCATC 
TACCTGCTGC CAGAGAGAAG ATGGGCGAGT CGGAAGAAGA CCTCAGTAAA ACAGAAGACC 
CTGGAGCCCC TATTCGATGA GACGTTTGAG TTTTTTGTCC CCATGGGAGA AGTGCAGAAG 
AGATCACTGG ATGTCGCAGT GAAAAACAGC AGGCCTCTCG GCTCACACAG AAGGAAGGAG 
TTGGGGAAAG TGCTGATTGA CTTATCAAAG CAAGATCTGA TTAAGGGCTT CTCACAGTGG 
TATGAACTGA CTGCAGATGG ACAGCCCAGA AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Esyt3 ( XM_006511221.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006511284.1 Mus musculus extended synaptotagmin-like protein 3 (Esyt3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu20770
Clone ID Related Accession (Same CDS sequence)	NM_177775.3 , XM_006511221.3 , XM_006511221.4
Accession Version	XM_006511221.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2676bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	extended synaptotagmin-3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006511221.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCCGG AGGAGCCCTG CGCCCCGAGC GCGCCCGGCG GTCCTGACGT CCCGGAGAGA 
GGCCAGCGCT CGCGGGACCC CGGACCGCGC CTGTCGGGCC AGCTACTGCC CGAGCTCTAC 
AGCTTCGTGG CGCGCGTCCT TTTCTACCTG GCACCGGTCT ACCTGGCGGG CTACCTGGGG 
CTCAGCGTCA CCTGGCTGCT GCTTGGCGCC CTGCTCTGGA TGTGGTGGCG CCGGAACCGC 
CGCGGGAAGC TGGGGCGCTT GGAGGCCGCC TTCGAATTCC TGGAGCACGA GCGCGAGTTC 
ATCAGCCGTG AGCTGCGGGG CCAGCATTTG CCAGCCTGGA TACATTTCCC AGATGTGGAG 
CGTGTGGAGT GGGCCAATAA GATCATCATA CAGATCTGGC CCTACCTAAG CATGATCATG 
GAGAACAAGA TCCGGGAGAA GCTGGAGCCC AAGATCCGGG AGAAGAGCAT CCACCTCAGA 
ACCTTCACCT TCACCAAGCT CTACTTTGGG CAGAAGTGTC CCAAGGTGAA CGGCGTCAAG 
GTACACACGG ACAAACGCAA CCGGAGGAAA GTGACCCTGG ACCTGCAGAT CTGCTACATC 
GGGGACTGTG AGATCAGCGT GGAACTTCAG AAGATCCGGG GCGGTGTGAG CGGCGTCCAG 
TTGCAGGGGA CCCTGCGTGT CATTCTGGAG CCCCTGCTGG TGGACAAGCC CTTCATAGGA 
GCTGTGACAG TGTTCTTCCT TCAAAAGCCG CACCTGCAGA TTAACTGGAC AGGCTTGACC 
AACCTGCTGG ACATGCCGGG GATCAATGAG TTATCAGACA GCCTGCTGGA GGACCTCATT 
GCTGCCCACC TGGTGCTGCC CAACCGTGTG ACTGTGCCTG TGAAGAAGGG GCTCGATGTA 
ACCAACCTGC GCGTCCCTCT CCCCTGTGGT GTGATCCGAG TCCATTTGCT GGAGGCCAAG 
AAGCTGGCCC AGAAGGACAA CTTCCTAGGG CTCGGAGGCA AGTCAGACCC CTACGCCAAG 
GTGAGCATTG GCTTGCAGCA TTGCCGGAGC AGAACCATCT ACAAGAACCT GAACCCCACC 
TGGAATGAAG TATTTGAGTT TATGGTGTAC GAGGTACCTG GGCAGGACCT GGAGGTGGAC 
CTGTATGATG AGGATACTGA CAAGGATGAC TTCCTGGGCA GCCTGCAGAT CTGCCTTGGA 
GATGTCATGA AGAACAGAGT GGTGGATGAG TGGTTTGCCC TGAATGACAC GACCAGTGGG 
CGGCTGCACC TGCGGCTGGA GTGGCTCTCA CTACTCACTG ACCAGGAAGC GCTGACGGAG 
AATGATAGTG GCCTCTCCAC TGCCATCCTC GTGGTCTTCT TAGAGAATGC CTGCAACCTG 
CCGAGAAACC CTTTTGACTA TCTGAATGGT GAATATCGAG CCAAAAAACT CTCCAGGTTT 
GTCAAAAATA AGGCCAGCAG AGACCCTTCT TCATACGTCA AGCTCACTGT AGGCAAGAAG 
ACCTTTACAA GTAAGACTTG TCCCCACAGC AAGGACCCTG TGTGGAGCCA GGTGTTCTCC 
TTCTTTGTGC ACAGCGTGGC AGCCGAGCAG CTCTGCCTGA AGGTGCTGGA CGATGAGCTA 
GAGTGTGCTC TGGGAGTGCT GGAGTTCCCC CTGTGCCGGA TCCTCCCCTG TGCTGACCTC 
ACCCTCGAAC AGTGCTTCCA GCTGGATCAC TCAGGCCTGG ACAGCCTCAT CTCCATGAGG 
CTGGTGCTTC GGTTCCTACG TGTGGAGGGA CGAGAGCTGG GAAGCCCATA CACTGGACCC 
GACGCTCTAA AGAAAGGTCC TCTGTTCATC AAGAAGGTGG CCACCAACCA GGGTTGTAAA 
GCCCCACCCC TAAACGAAGG CCTTGCAGAT GTGACAAGCA CCTCGAACCC TGCCTCTTAC 
ATCAAAGGAG CCTCCAAGAG CATTGACAAT ATCAGCGCAG CCACCACAGA CCCTGAACCT 
ATGCCTGAGC CCCAGGGGCC AGGCCCGGAG CCCAAAGGCA AGGACAGTGC CAGAGGGCTC 
TGTGAGTCCC CTGGAAAGAA GAAGAACCCA GCTACCACCT TCCTGACTGT CCCAGGTCTC 
CACTCTCCAG GGCCCATCAA GTCACCCAGA CCCATGAGCC GCCCTGCCTT CCCATTCGCA 
TGGCCACTCA CGAGGGTGGC TCCCAGCATG TCCTCACTCA ACTCCCTGGC CTCTTCCTGC 
TTTGACCTGA CAGATGTCAG CCTCAACACT GAAGCTGGGG ATTCCAGGCA GGGACGGCTG 
GGTGAGATTC AGCTCACAGT GCGCTATGTG TGTCTCCGAC ACTGTCTCAG AGTGCTGGTC 
AACGGCTGCA GAAACTTAAC ACCATGCACC AGCAGTGGAG CTGACCCCTA CGTCCGCATC 
TACCTGCTGC CAGAGAGAAG ATGGGCGAGT CGGAAGAAGA CCTCAGTAAA ACAGAAGACC 
CTGGAGCCCC TATTCGATGA GACGTTTGAG TTTTTTGTCC CCATGGGAGA AGTGCAGAAG 
AGATCACTGG ATGTCGCAGT GAAAAACAGC AGGCCTCTCG GCTCACACAG AAGGAAGGAG 
TTGGGGAAAG TGCTGATTGA CTTATCAAAG CAAGATCTGA TTAAGGGCTT CTCACAGTGG 
TATGAACTGA CTGCAGATGG ACAGCCCAGA AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006511284.1
CDS	841..3516
Translation	MQPEEPCAPSAPGGPDVPERGQRSRDPGPRLSGQLLPELYSFVARVLFYLAPVYLAGYLGLSVTWLLLGALLWMWWRRNRRGKLGRLEAAFEFLEHEREFISRELRGQHLPAWIHFPDVERVEWANKIIIQIWPYLSMIMENKIREKLEPKIREKSIHLRTFTFTKLYFGQKCPKVNGVKVHTDKRNRRKVTLDLQICYIGDCEISVELQKIRGGVSGVQLQGTLRVILEPLLVDKPFIGAVTVFFLQKPHLQINWTGLTNLLDMPGINELSDSLLEDLIAAHLVLPNRVTVPVKKGLDVTNLRVPLPCGVIRVHLLEAKKLAQKDNFLGLGGKSDPYAKVSIGLQHCRSRTIYKNLNPTWNEVFEFMVYEVPGQDLEVDLYDEDTDKDDFLGSLQICLGDVMKNRVVDEWFALNDTTSGRLHLRLEWLSLLTDQEALTENDSGLSTAILVVFLENACNLPRNPFDYLNGEYRAKKLSRFVKNKASRDPSSYVKLTVGKKTFTSKTCPHSKDPVWSQVFSFFVHSVAAEQLCLKVLDDELECALGVLEFPLCRILPCADLTLEQCFQLDHSGLDSLISMRLVLRFLRVEGRELGSPYTGPDALKKGPLFIKKVATNQGCKAPPLNEGLADVTSTSNPASYIKGASKSIDNISAATTDPEPMPEPQGPGPEPKGKDSARGLCESPGKKKNPATTFLTVPGLHSPGPIKSPRPMSRPAFPFAWPLTRVAPSMSSLNSLASSCFDLTDVSLNTEAGDSRQGRLGEIQLTVRYVCLRHCLRVLVNGCRNLTPCTSSGADPYVRIYLLPERRWASRKKTSVKQKTLEPLFDETFEFFVPMGEVQKRSLDVAVKNSRPLGSHRRKELGKVLIDLSKQDLIKGFSQWYELTADGQPRS

Target ORF information:

RefSeq Version	XM_006511221.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus extended synaptotagmin-like protein 3 (Esyt3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006511221.4

ORF Insert Sequence:

ATGCAGCCGG AGGAGCCCTG CGCCCCGAGC GCGCCCGGCG GTCCTGACGT CCCGGAGAGA 
GGCCAGCGCT CGCGGGACCC CGGACCGCGC CTGTCGGGCC AGCTACTGCC CGAGCTCTAC 
AGCTTCGTGG CGCGCGTCCT TTTCTACCTG GCACCGGTCT ACCTGGCGGG CTACCTGGGG 
CTCAGCGTCA CCTGGCTGCT GCTTGGCGCC CTGCTCTGGA TGTGGTGGCG CCGGAACCGC 
CGCGGGAAGC TGGGGCGCTT GGAGGCCGCC TTCGAATTCC TGGAGCACGA GCGCGAGTTC 
ATCAGCCGTG AGCTGCGGGG CCAGCATTTG CCAGCCTGGA TACATTTCCC AGATGTGGAG 
CGTGTGGAGT GGGCCAATAA GATCATCATA CAGATCTGGC CCTACCTAAG CATGATCATG 
GAGAACAAGA TCCGGGAGAA GCTGGAGCCC AAGATCCGGG AGAAGAGCAT CCACCTCAGA 
ACCTTCACCT TCACCAAGCT CTACTTTGGG CAGAAGTGTC CCAAGGTGAA CGGCGTCAAG 
GTACACACGG ACAAACGCAA CCGGAGGAAA GTGACCCTGG ACCTGCAGAT CTGCTACATC 
GGGGACTGTG AGATCAGCGT GGAACTTCAG AAGATCCGGG GCGGTGTGAG CGGCGTCCAG 
TTGCAGGGGA CCCTGCGTGT CATTCTGGAG CCCCTGCTGG TGGACAAGCC CTTCATAGGA 
GCTGTGACAG TGTTCTTCCT TCAAAAGCCG CACCTGCAGA TTAACTGGAC AGGCTTGACC 
AACCTGCTGG ACATGCCGGG GATCAATGAG TTATCAGACA GCCTGCTGGA GGACCTCATT 
GCTGCCCACC TGGTGCTGCC CAACCGTGTG ACTGTGCCTG TGAAGAAGGG GCTCGATGTA 
ACCAACCTGC GCGTCCCTCT CCCCTGTGGT GTGATCCGAG TCCATTTGCT GGAGGCCAAG 
AAGCTGGCCC AGAAGGACAA CTTCCTAGGG CTCGGAGGCA AGTCAGACCC CTACGCCAAG 
GTGAGCATTG GCTTGCAGCA TTGCCGGAGC AGAACCATCT ACAAGAACCT GAACCCCACC 
TGGAATGAAG TATTTGAGTT TATGGTGTAC GAGGTACCTG GGCAGGACCT GGAGGTGGAC 
CTGTATGATG AGGATACTGA CAAGGATGAC TTCCTGGGCA GCCTGCAGAT CTGCCTTGGA 
GATGTCATGA AGAACAGAGT GGTGGATGAG TGGTTTGCCC TGAATGACAC GACCAGTGGG 
CGGCTGCACC TGCGGCTGGA GTGGCTCTCA CTACTCACTG ACCAGGAAGC GCTGACGGAG 
AATGATAGTG GCCTCTCCAC TGCCATCCTC GTGGTCTTCT TAGAGAATGC CTGCAACCTG 
CCGAGAAACC CTTTTGACTA TCTGAATGGT GAATATCGAG CCAAAAAACT CTCCAGGTTT 
GTCAAAAATA AGGCCAGCAG AGACCCTTCT TCATACGTCA AGCTCACTGT AGGCAAGAAG 
ACCTTTACAA GTAAGACTTG TCCCCACAGC AAGGACCCTG TGTGGAGCCA GGTGTTCTCC 
TTCTTTGTGC ACAGCGTGGC AGCCGAGCAG CTCTGCCTGA AGGTGCTGGA CGATGAGCTA 
GAGTGTGCTC TGGGAGTGCT GGAGTTCCCC CTGTGCCGGA TCCTCCCCTG TGCTGACCTC 
ACCCTCGAAC AGTGCTTCCA GCTGGATCAC TCAGGCCTGG ACAGCCTCAT CTCCATGAGG 
CTGGTGCTTC GGTTCCTACG TGTGGAGGGA CGAGAGCTGG GAAGCCCATA CACTGGACCC 
GACGCTCTAA AGAAAGGTCC TCTGTTCATC AAGAAGGTGG CCACCAACCA GGGTTGTAAA 
GCCCCACCCC TAAACGAAGG CCTTGCAGAT GTGACAAGCA CCTCGAACCC TGCCTCTTAC 
ATCAAAGGAG CCTCCAAGAG CATTGACAAT ATCAGCGCAG CCACCACAGA CCCTGAACCT 
ATGCCTGAGC CCCAGGGGCC AGGCCCGGAG CCCAAAGGCA AGGACAGTGC CAGAGGGCTC 
TGTGAGTCCC CTGGAAAGAA GAAGAACCCA GCTACCACCT TCCTGACTGT CCCAGGTCTC 
CACTCTCCAG GGCCCATCAA GTCACCCAGA CCCATGAGCC GCCCTGCCTT CCCATTCGCA 
TGGCCACTCA CGAGGGTGGC TCCCAGCATG TCCTCACTCA ACTCCCTGGC CTCTTCCTGC 
TTTGACCTGA CAGATGTCAG CCTCAACACT GAAGCTGGGG ATTCCAGGCA GGGACGGCTG 
GGTGAGATTC AGCTCACAGT GCGCTATGTG TGTCTCCGAC ACTGTCTCAG AGTGCTGGTC 
AACGGCTGCA GAAACTTAAC ACCATGCACC AGCAGTGGAG CTGACCCCTA CGTCCGCATC 
TACCTGCTGC CAGAGAGAAG ATGGGCGAGT CGGAAGAAGA CCTCAGTAAA ACAGAAGACC 
CTGGAGCCCC TATTCGATGA GACGTTTGAG TTTTTTGTCC CCATGGGAGA AGTGCAGAAG 
AGATCACTGG ATGTCGCAGT GAAAAACAGC AGGCCTCTCG GCTCACACAG AAGGAAGGAG 
TTGGGGAAAG TGCTGATTGA CTTATCAAAG CAAGATCTGA TTAAGGGCTT CTCACAGTGG 
TATGAACTGA CTGCAGATGG ACAGCCCAGA AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Loss of all 3 Extended Synaptotagmins does not affect normal mouse development, viability or fertility.
Cell cycle (Georgetown, Tex.)15(17)2360-6(2016 Sep)
Tremblay MG,Moss T

Extended Synaptotagmin (ESyt) Triple Knock-Out Mice Are Viable and Fertile without Obvious Endoplasmic Reticulum Dysfunction.
PloS one11(6)e0158295(2016)
Sclip A,Bacaj T,Giam LR,S?dhof TC

Loss of Extended Synaptotagmins ESyt2 and ESyt3 does not affect mouse development or viability, but in vitro cell migration and survival under stress are affected.
Cell cycle (Georgetown, Tex.)13(16)2616-25(2014)
Herdman C,Tremblay MG,Mishra PK,Moss T

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

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Esyt3 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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