Atp1a4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Atp1a4
Entrez Gene ID	27222
Full Name	ATPase, Na+/K+ transporting, alpha 4 polypeptide
General protein information	Preferred Names ATPase, Na+/K+ transporting, alpha 4 polypeptide Names sodium/potassium-transporting ATPase subunit alpha-4 Na(+)/K(+) ATPase alpha-4 subunit Na+/K+ ATPase alpha 4 subunit sodium pump subunit alpha-4 sodium/potassium-transporting ATPase alpha-4 chain
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1 H3\|1 79.54 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006496874.2	XP_006496937.1	sodium/potassium-transporting ATPase subunit alpha-4 isoform X1
NM_013734.1	NP_038762.1	sodium/potassium-transporting ATPase subunit alpha-4

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Atp1a4	NP_001257959.1
Homo sapiens (human)	ATP1A4	NP_653300.2
Pan troglodytes (chimpanzee)	ATP1A4	XP_001171135.1
	ATP1A4	XP_001117376.1
Canis lupus familiaris (dog)	ATP1A4	XP_545754.3
Mus musculus (house mouse)	Atp1a4	NP_038762.1
Bos taurus (cattle)	ATP1A4	NP_001137575.1

Gene Ontology
Bibliography

Related articles in PubMed

Daple Coordinates Planar Polarized Microtubule Dynamics in Ependymal Cells and Contributes to Hydrocephalus.
Takagishi M, Sawada M, Ohata S, Asai N, Enomoto A, Takahashi K, Weng L, Ushida K, Ara H, Matsui S, Kaibuchi K, Sawamoto K, Takahashi M
Cell reports20(4)960-972(2017 Jul)

Ouabain interactions with the ?4 isoform of the sodium pump trigger non-classical steroid hormone signaling and integrin expression in spermatogenic cells.
Upmanyu N, Dietze R, Kirch U, Scheiner-Bobis G
Biochimica et biophysica acta1863(11)2809-2819(2016 11)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Methylation-dependent and independent regulatory regions in the Na,K-ATPase alpha4 (Atp1a4) gene may impact its testis-specific expression.
Kumar DL, Kumar PL, James PF
Gene575(2 Pt 1)339-52(2016 Jan)

Green fluorescence protein driven by the Na,K-ATPase ?4 isoform promoter is expressed only in male germ cells of mouse testis.
McDermott JP, S?nchez G, Chennathukuzhi V, Blanco G
Journal of assisted reproduction and genetics29(12)1313-25(2012 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Ouabain interactions with the alpha4 isoform of the sodium pump trigger non-classical steroid hormone signaling and integrin expression in spermatocytes.
Title: Ouabain interactions with the ?4 isoform of the sodium pump trigger non-classical steroid hormone signaling and integrin expression in spermatogenic cells.

data suggests that both the promoter and the intragenic CGI could combine to provide multiple modes of regulation for optimizing the Atp1a4 expression level in a cell type-specific manner.
Title: Methylation-dependent and independent regulatory regions in the Na,K-ATPase alpha4 (Atp1a4) gene may impact its testis-specific expression.

These findings highlight the exquisite spatial and temporal control of expression exerted by the Atp1a4 promoter on Na,K-ATPase alpha4, which is particularly well suited to fulfill the special functions of spermatozoa.
Title: Green fluorescence protein driven by the Na,K-ATPase ?4 isoform promoter is expressed only in male germ cells of mouse testis.

Na,K-ATPase alpha4 isoform is essential for sperm fertility
Title: Na,K-ATPase alpha4 isoform is essential for sperm fertility.

The following Atp1a4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Atp1a4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu28772	XM_006496874.2 Latest version!	Mus musculus ATPase, Na+/K+ transporting, alpha 4 polypeptide (Atp1a4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu09858	NM_013734.1 Latest version!	Mus musculus ATPase, Na+/K+ transporting, alpha 4 polypeptide (Atp1a4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Atp1a4 ( XM_006496874.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496937.1 Mus musculus ATPase, Na+/K+ transporting, alpha 4 polypeptide (Atp1a4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu28772
Clone ID Related Accession (Same CDS sequence)	XM_006496874.2
Accession Version	XM_006496874.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2187bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	sodium/potassium-transporting ATPase subunit alpha-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006496874.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAAGA GGCTCCTGAA TGAAAGATCT AAATGCGTCC CGGGGAAGGA GCCTGTGCTC 
TGTGACAGAC ACAGGAGTGA GGCGCACACT GAAAACTCTC GCCATAGACC ATTTCCTCAG 
CATCCCTTAC AGAGATCACA GGTGTGCCTG ACGCTGACCG CCAAGCGCAT GGCTCGCAAG 
AACTGCCTGG TGAAGAACCT GGAGGCCGTG GAGACGCTGG GCTCCACGTC CACCATCTGC 
TCAGACAAGA CGGGCACCCT CACCCAGAAC CGCATGACAG TGGCGCATTT GTGGTTTGAT 
AAGACTGTGT ATGAGGCCGA CACCAGTGAG GAGCAAACCA CGGGGAAAAC GTTTCCCAAG 
TCCTCAGACA CCTGGTTTTA CCTGGCTCGA ATCGCTGGCC TCTGTAACCG GGCCGACTTT 
AAACCCCATC AGGAGTCTGT TCCCATAGCT AAGCGGGCAA CAACAGGGGA TGCTTCCGAG 
TCAGCTCTCC TCAAATTCAT TGAGCAGTCT TACAACCCCG TGAGCGAAAT GAGGCAGAAA 
AACCCCAAGG TGGCGGAGAT TCCCTTTAAC TCCACCAACA AGTATCAGAT GTCCATCCAC 
CTTCTGGAAG ATAATTCCGA AGCCCACGTG CTGCTAATGA AAGGTGCCCC AGAGAGGATC 
TTTGATTTTT GCTCCAGTTT CCTGCTGAAT GGGCAGGAGT ACCCTATGGA TGAGGAAATG 
AAGATGGACT TCCAGAATGC CTACATAGAA CTGGGAGGTC TGGGCGAACG TGTGCTGGGG 
TTCTGCTTCT TGAACTTGCC TAGCAATTTC TCTAAGGGGT TCCAGTTTAA TACCGATGAG 
TTAAATTTTC CCATGGAAAA TCTGTGTTTC GCGGGCCTCA TCTCCATGAT TGATCCTCCT 
CGGACGGCCG TGCCTGACGC TGTGAGCAAA TGTCGAAGCG CGGGGATCAA GGTGATCATG 
GTAACGGGGG ATCATCCCAT CACAGCCAAA GCTATTGCCA AAAGTGTGGG TATCATTTCA 
GAAGGTAACG ACACGGCGGA GGACATTGCC GCTCGGCTGA ATATCCCTAT CAGTCAAGTC 
AATAACAAGT CTGTTAAAGC CATTGTAGTG CACGGCTCAG AACTGAAAGA CATGGAATCC 
CAACAGCTGG ACGACATCCT CAAGTCCTAT AAGGAGATTG TCTTTGCCCG GACCTCTCCT 
CAGCAGAAAC TCATCATTGT GGAGGGGTGT CAGCGACTGG GAGCCATTGT GGCAGTGACT 
GGTGACGGGG TGAACGACTC CCCCGCACTG AAGAAGGCTG ACATCGGCAT TGCCATGGGC 
ATCACAGGCT CTGATGTCTC TAAACAGGCG GCTGACATGA TCCTTCTTGA CGACAATTTT 
GCCTCCATTG TGACAGGCGT GGAGGAGGGC CGCCTCATCT TTGACAACCT GAAGAAGTCC 
ATCGCATACA CCCTGACCAG CAACATCCCT GAGATCACCC CCTTCCTGCT GTTCATCATC 
TTAAGCATAC CCTTGCCTCT GGGCACCATA ACCATTCTCT GCATCGACCT GGGCACTGAC 
ATGGTCCCTG CTATCTCCCT GGCTTATGAA TCACCTGAAA GTGACATCAT GAAAAGGCTT 
CCACGGAACC CCAAAACGGA CAATCTTGTG AACAACCGTC TTATTGGCAT GGCCTATGGG 
CAGATTGGGA TGATACAGGC TCTGGCTGGA TTTTTCACCT ATTTTGTGAT CCTGGCGGAG 
AATGGTTTTA AGCCTCTTGA TCTTCTGGGT ATCCGTCTAT ACTGGGATGA TACACAATTA 
AATGACCTAG AGGACAGCTA TGGGCAGCAG TGGACCTATG AGCAGCGGAA GGTGGTGGAA 
TTCACATGCC AAACGGCCTT CTTCATCAGT ATCGTGATTG TGCAGTGGGC TGACCTCATC 
ATCTGCAAGA CACGCAGAAA TTCACTCTTC AAGCAGGGCA TGAAAAACAA AATCCTAATA 
TTTGGACTCC TTGAGGAGAC AGTCCTGGCA GCTTTCCTGT CCTACGTTCC AGGCATGGAT 
GTGTCCCTGC GGATGTACCC ACTCAAGATA AACTGGTGGT TCTGCGCACT TCCCTACAGT 
GTTCTCATCT TCGTCTATGA TGAAATCAGA AAACTTATCA TCCGGAGACG GCCTGGTGGA 
TGGCTGGAGA AGGAGACGTA CTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496937.1
CDS	66..2252
Translation	MEKRLLNERSKCVPGKEPVLCDRHRSEAHTENSRHRPFPQHPLQRSQVCLTLTAKRMARKNCLVKNLEAVETLGSTSTICSDKTGTLTQNRMTVAHLWFDKTVYEADTSEEQTTGKTFPKSSDTWFYLARIAGLCNRADFKPHQESVPIAKRATTGDASESALLKFIEQSYNPVSEMRQKNPKVAEIPFNSTNKYQMSIHLLEDNSEAHVLLMKGAPERIFDFCSSFLLNGQEYPMDEEMKMDFQNAYIELGGLGERVLGFCFLNLPSNFSKGFQFNTDELNFPMENLCFAGLISMIDPPRTAVPDAVSKCRSAGIKVIMVTGDHPITAKAIAKSVGIISEGNDTAEDIAARLNIPISQVNNKSVKAIVVHGSELKDMESQQLDDILKSYKEIVFARTSPQQKLIIVEGCQRLGAIVAVTGDGVNDSPALKKADIGIAMGITGSDVSKQAADMILLDDNFASIVTGVEEGRLIFDNLKKSIAYTLTSNIPEITPFLLFIILSIPLPLGTITILCIDLGTDMVPAISLAYESPESDIMKRLPRNPKTDNLVNNRLIGMAYGQIGMIQALAGFFTYFVILAENGFKPLDLLGIRLYWDDTQLNDLEDSYGQQWTYEQRKVVEFTCQTAFFISIVIVQWADLIICKTRRNSLFKQGMKNKILIFGLLEETVLAAFLSYVPGMDVSLRMYPLKINWWFCALPYSVLIFVYDEIRKLIIRRRPGGWLEKETYY

Target ORF information:

RefSeq Version	XM_006496874.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ATPase, Na+/K+ transporting, alpha 4 polypeptide (Atp1a4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496874.2

ORF Insert Sequence:

ATGGAAAAGA GGCTCCTGAA TGAAAGATCT AAATGCGTCC CGGGGAAGGA GCCTGTGCTC 
TGTGACAGAC ACAGGAGTGA GGCGCACACT GAAAACTCTC GCCATAGACC ATTTCCTCAG 
CATCCCTTAC AGAGATCACA GGTGTGCCTG ACGCTGACCG CCAAGCGCAT GGCTCGCAAG 
AACTGCCTGG TGAAGAACCT GGAGGCCGTG GAGACGCTGG GCTCCACGTC CACCATCTGC 
TCAGACAAGA CGGGCACCCT CACCCAGAAC CGCATGACAG TGGCGCATTT GTGGTTTGAT 
AAGACTGTGT ATGAGGCCGA CACCAGTGAG GAGCAAACCA CGGGGAAAAC GTTTCCCAAG 
TCCTCAGACA CCTGGTTTTA CCTGGCTCGA ATCGCTGGCC TCTGTAACCG GGCCGACTTT 
AAACCCCATC AGGAGTCTGT TCCCATAGCT AAGCGGGCAA CAACAGGGGA TGCTTCCGAG 
TCAGCTCTCC TCAAATTCAT TGAGCAGTCT TACAACCCCG TGAGCGAAAT GAGGCAGAAA 
AACCCCAAGG TGGCGGAGAT TCCCTTTAAC TCCACCAACA AGTATCAGAT GTCCATCCAC 
CTTCTGGAAG ATAATTCCGA AGCCCACGTG CTGCTAATGA AAGGTGCCCC AGAGAGGATC 
TTTGATTTTT GCTCCAGTTT CCTGCTGAAT GGGCAGGAGT ACCCTATGGA TGAGGAAATG 
AAGATGGACT TCCAGAATGC CTACATAGAA CTGGGAGGTC TGGGCGAACG TGTGCTGGGG 
TTCTGCTTCT TGAACTTGCC TAGCAATTTC TCTAAGGGGT TCCAGTTTAA TACCGATGAG 
TTAAATTTTC CCATGGAAAA TCTGTGTTTC GCGGGCCTCA TCTCCATGAT TGATCCTCCT 
CGGACGGCCG TGCCTGACGC TGTGAGCAAA TGTCGAAGCG CGGGGATCAA GGTGATCATG 
GTAACGGGGG ATCATCCCAT CACAGCCAAA GCTATTGCCA AAAGTGTGGG TATCATTTCA 
GAAGGTAACG ACACGGCGGA GGACATTGCC GCTCGGCTGA ATATCCCTAT CAGTCAAGTC 
AATAACAAGT CTGTTAAAGC CATTGTAGTG CACGGCTCAG AACTGAAAGA CATGGAATCC 
CAACAGCTGG ACGACATCCT CAAGTCCTAT AAGGAGATTG TCTTTGCCCG GACCTCTCCT 
CAGCAGAAAC TCATCATTGT GGAGGGGTGT CAGCGACTGG GAGCCATTGT GGCAGTGACT 
GGTGACGGGG TGAACGACTC CCCCGCACTG AAGAAGGCTG ACATCGGCAT TGCCATGGGC 
ATCACAGGCT CTGATGTCTC TAAACAGGCG GCTGACATGA TCCTTCTTGA CGACAATTTT 
GCCTCCATTG TGACAGGCGT GGAGGAGGGC CGCCTCATCT TTGACAACCT GAAGAAGTCC 
ATCGCATACA CCCTGACCAG CAACATCCCT GAGATCACCC CCTTCCTGCT GTTCATCATC 
TTAAGCATAC CCTTGCCTCT GGGCACCATA ACCATTCTCT GCATCGACCT GGGCACTGAC 
ATGGTCCCTG CTATCTCCCT GGCTTATGAA TCACCTGAAA GTGACATCAT GAAAAGGCTT 
CCACGGAACC CCAAAACGGA CAATCTTGTG AACAACCGTC TTATTGGCAT GGCCTATGGG 
CAGATTGGGA TGATACAGGC TCTGGCTGGA TTTTTCACCT ATTTTGTGAT CCTGGCGGAG 
AATGGTTTTA AGCCTCTTGA TCTTCTGGGT ATCCGTCTAT ACTGGGATGA TACACAATTA 
AATGACCTAG AGGACAGCTA TGGGCAGCAG TGGACCTATG AGCAGCGGAA GGTGGTGGAA 
TTCACATGCC AAACGGCCTT CTTCATCAGT ATCGTGATTG TGCAGTGGGC TGACCTCATC 
ATCTGCAAGA CACGCAGAAA TTCACTCTTC AAGCAGGGCA TGAAAAACAA AATCCTAATA 
TTTGGACTCC TTGAGGAGAC AGTCCTGGCA GCTTTCCTGT CCTACGTTCC AGGCATGGAT 
GTGTCCCTGC GGATGTACCC ACTCAAGATA AACTGGTGGT TCTGCGCACT TCCCTACAGT 
GTTCTCATCT TCGTCTATGA TGAAATCAGA AAACTTATCA TCCGGAGACG GCCTGGTGGA 
TGGCTGGAGA AGGAGACGTA CTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Atp1a4 ( NM_013734.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_038762.1 Mus musculus ATPase, Na+/K+ transporting, alpha 4 polypeptide (Atp1a4), mRNA.

Price & Availability↑

CloneID	OMu09858
Clone ID Related Accession (Same CDS sequence)	NM_013734.1
Accession Version	NM_013734.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3099bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	sodium/potassium-transporting ATPase subunit alpha-4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC087061.22 and AC074310.29. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849384 [ECO:0000348] ##Evidence-Data-END##

ATGGAGCCTG GAAAAGAAAA GGAGGTAGAG GCCCCTGGTG AGCTGAATCA GAAACCAAGA 
CCCAGCACCA GGTCCTCAAC TACAAATAGA CAGCCGAAAA TGAAAAGAAG GAAGAAAGAT 
TTAGAAGAGC TGAAGAAGGA AGTGGTTATG GATGATCACA AGCTAACGTT GGATGAGCTG 
AGTGCCAAGT ACTCTGTGGA CTTGACCAAG GGTCTTAGCA TCCTAGAAGC CCAGGATATT 
CTGTTTCAAA ATGGACCGAA TGTACTAACT CCTCCTCCAA CCACTCCAGA GTGGGTCAAA 
TTCTGTAGAC AACTGTTTGG TGGCTTCTCC CTCCTACTGT GGACTGGTGC CTGTCTCTGC 
TTCCTGGCCT ATGGCATACA TGTAAATTAT TACAAAGAAA ACGCCAACAA AGATAATCTG 
TACCTGGGGA TTGTACTATC TGCCGTGGTC ATCATTACCG GCTGCTTCTC ATATTACCAG 
GAGGCCAAGA GCTCCAAGAT CATGGAGTCT TTTAAGAACA TGGTGCCTCA GCAAGCTCTG 
GTGATTCGAG ATGGAGAGAA AATGCAAATC AATGTAAGAG ATGTGGTTTT GGGAGACCTA 
GTAGAGGTGA AGGGAGGAGA CCAAATCCCT GCTGATATCA GGGTTATCTC GGCGCAAGGA 
TGCAAGGTAG ACAATTCCTC CTTGACTGGG GAGTCAGAAC CCCAGTCTCG CTGCCCTGAT 
TGCACTCATG AGAACCCTCT GGAGACACGG AATATCATCT TCTTCTCCAC TAACTGTGTA 
GAAGGAACAG CTCGGGGTAT TGTGATTGCT ACAGGAGACT ACACAGTAAT GGGCCGGATT 
GCTTCCCTGA CATCAGGCTT GCAAATGGGC AAGACCCCGA TTGCCACCGA AATCGAACAC 
TTCATCCATT TGATCACAGC TGTCGCGGTC TTCCTCGGTG TTTCATTTTT TTGGCTCTCG 
ATTATCTTGG GTTACACCTG GCTGGATGCT GTCATTTTCC TTATTGGTAT CATTGTGGCC 
AATGTGCCGG AAGGGCTGCT GGCCACTGTC ACTGTGTGCC TGACGCTGAC CGCCAAGCGC 
ATGGCTCGCA AGAACTGCCT GGTGAAGAAC CTGGAGGCCG TGGAGACGCT GGGCTCCACG 
TCCACCATCT GCTCAGACAA GACGGGCACC CTCACCCAGA ACCGCATGAC AGTGGCGCAT 
TTGTGGTTTG ATAAGACTGT GTATGAGGCC GACACCAGTG AGGAGCAAAC CACGGGGAAA 
ACGTTTCCCA AGTCCTCAGA CACCTGGTTT TACCTGGCTC GAATCGCTGG CCTCTGTAAC 
CGGGCCGACT TTAAACCCCA TCAGGAGTCT GTTCCCATAG CTAAGCGGGC AACAACAGGG 
GATGCTTCCG AGTCAGCTCT CCTCAAATTC ATTGAGCAGT CTTACAACCC CGTGAGCGAA 
ATGAGGCAGA AAAACCCCAA GGTGGCGGAG ATTCCCTTTA ACTCCACCAA CAAGTATCAG 
ATGTCCATCC ACCTTCTGGA AGATAATTCC GAAGCCCACG TGCTGCTAAT GAAAGGTGCC 
CCAGAGAGGA TCTTTGATTT TTGCTCCAGT TTCCTGCTGA ATGGGCAGGA GTACCCTATG 
GATGAGGAAA TGAAGATGGA CTTCCAGAAT GCCTACATAG AACTGGGAGG TCTGGGCGAA 
CGTGTGCTGG GGTTCTGCTT CTTGAACTTG CCTAGCAATT TCTCTAAGGG GTTCCAGTTT 
AATACCGATG AGTTAAATTT TCCCATGGAA AATCTGTGTT TCGCGGGCCT CATCTCCATG 
ATTGATCCTC CTCGGACGGC CGTGCCTGAC GCTGTGAGCA AATGTCGAAG CGCGGGGATC 
AAGGTGATCA TGGTAACGGG GGATCATCCC ATCACAGCCA AAGCTATTGC CAAAAGTGTG 
GGTATCATTT CAGAAGGTAA CGACACGGCG GAGGACATTG CCGCTCGGCT GAATATCCCT 
ATCAGTCAAG TCAATAACAA GTCTGTTAAA GCCATTGTAG TGCACGGCTC AGAACTGAAA 
GACATGGAAT CCCAACAGCT GGACGACATC CTCAAGTCCT ATAAGGAGAT TGTCTTTGCC 
CGGACCTCTC CTCAGCAGAA ACTCATCATT GTGGAGGGGT GTCAGCGACT GGGAGCCATT 
GTGGCAGTGA CTGGTGACGG GGTGAACGAC TCCCCCGCAC TGAAGAAGGC TGACATCGGC 
ATTGCCATGG GCATCACAGG CTCTGATGTC TCTAAACAGG CGGCTGACAT GATCCTTCTT 
GACGACAATT TTGCCTCCAT TGTGACAGGC GTGGAGGAGG GCCGCCTCAT CTTTGACAAC 
CTGAAGAAGT CCATCGCATA CACCCTGACC AGCAACATCC CTGAGATCAC CCCCTTCCTG 
CTGTTCATCA TCTTAAGCAT ACCCTTGCCT CTGGGCACCA TAACCATTCT CTGCATCGAC 
CTGGGCACTG ACATGGTCCC TGCTATCTCC CTGGCTTATG AATCACCTGA AAGTGACATC 
ATGAAAAGGC TTCCACGGAA CCCCAAAACG GACAATCTTG TGAACAACCG TCTTATTGGC 
ATGGCCTATG GGCAGATTGG GATGATACAG GCTCTGGCTG GATTTTTCAC CTATTTTGTG 
ATCCTGGCGG AGAATGGTTT TAAGCCTCTT GATCTTCTGG GTATCCGTCT ATACTGGGAT 
GATACACAAT TAAATGACCT AGAGGACAGC TATGGGCAGC AGTGGACCTA TGAGCAGCGG 
AAGGTGGTGG AATTCACATG CCAAACGGCC TTCTTCATCA GTATCGTGAT TGTGCAGTGG 
GCTGACCTCA TCATCTGCAA GACACGCAGA AATTCACTCT TCAAGCAGGG CATGAAAAAC 
AAAATCCTAA TATTTGGACT CCTTGAGGAG ACAGTCCTGG CAGCTTTCCT GTCCTACGTT 
CCAGGCATGG ATGTGTCCCT GCGGATGTAC CCACTCAAGA TAAACTGGTG GTTCTGCGCA 
CTTCCCTACA GTGTTCTCAT CTTCGTCTAT GATGAAATCA GAAAACTTAT CATCCGGAGA 
CGGCCTGGTG GATGGCTGGA GAAGGAGACG TACTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038762.1
CDS	97..3195
Translation	MEPGKEKEVEAPGELNQKPRPSTRSSTTNRQPKMKRRKKDLEELKKEVVMDDHKLTLDELSAKYSVDLTKGLSILEAQDILFQNGPNVLTPPPTTPEWVKFCRQLFGGFSLLLWTGACLCFLAYGIHVNYYKENANKDNLYLGIVLSAVVIITGCFSYYQEAKSSKIMESFKNMVPQQALVIRDGEKMQINVRDVVLGDLVEVKGGDQIPADIRVISAQGCKVDNSSLTGESEPQSRCPDCTHENPLETRNIIFFSTNCVEGTARGIVIATGDYTVMGRIASLTSGLQMGKTPIATEIEHFIHLITAVAVFLGVSFFWLSIILGYTWLDAVIFLIGIIVANVPEGLLATVTVCLTLTAKRMARKNCLVKNLEAVETLGSTSTICSDKTGTLTQNRMTVAHLWFDKTVYEADTSEEQTTGKTFPKSSDTWFYLARIAGLCNRADFKPHQESVPIAKRATTGDASESALLKFIEQSYNPVSEMRQKNPKVAEIPFNSTNKYQMSIHLLEDNSEAHVLLMKGAPERIFDFCSSFLLNGQEYPMDEEMKMDFQNAYIELGGLGERVLGFCFLNLPSNFSKGFQFNTDELNFPMENLCFAGLISMIDPPRTAVPDAVSKCRSAGIKVIMVTGDHPITAKAIAKSVGIISEGNDTAEDIAARLNIPISQVNNKSVKAIVVHGSELKDMESQQLDDILKSYKEIVFARTSPQQKLIIVEGCQRLGAIVAVTGDGVNDSPALKKADIGIAMGITGSDVSKQAADMILLDDNFASIVTGVEEGRLIFDNLKKSIAYTLTSNIPEITPFLLFIILSIPLPLGTITILCIDLGTDMVPAISLAYESPESDIMKRLPRNPKTDNLVNNRLIGMAYGQIGMIQALAGFFTYFVILAENGFKPLDLLGIRLYWDDTQLNDLEDSYGQQWTYEQRKVVEFTCQTAFFISIVIVQWADLIICKTRRNSLFKQGMKNKILIFGLLEETVLAAFLSYVPGMDVSLRMYPLKINWWFCALPYSVLIFVYDEIRKLIIRRRPGGWLEKETYY

Target ORF information:

RefSeq Version	NM_013734.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ATPase, Na+/K+ transporting, alpha 4 polypeptide (Atp1a4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013734.1

ORF Insert Sequence:

ATGGAGCCTG GAAAAGAAAA GGAGGTAGAG GCCCCTGGTG AGCTGAATCA GAAACCAAGA 
CCCAGCACCA GGTCCTCAAC TACAAATAGA CAGCCGAAAA TGAAAAGAAG GAAGAAAGAT 
TTAGAAGAGC TGAAGAAGGA AGTGGTTATG GATGATCACA AGCTAACGTT GGATGAGCTG 
AGTGCCAAGT ACTCTGTGGA CTTGACCAAG GGTCTTAGCA TCCTAGAAGC CCAGGATATT 
CTGTTTCAAA ATGGACCGAA TGTACTAACT CCTCCTCCAA CCACTCCAGA GTGGGTCAAA 
TTCTGTAGAC AACTGTTTGG TGGCTTCTCC CTCCTACTGT GGACTGGTGC CTGTCTCTGC 
TTCCTGGCCT ATGGCATACA TGTAAATTAT TACAAAGAAA ACGCCAACAA AGATAATCTG 
TACCTGGGGA TTGTACTATC TGCCGTGGTC ATCATTACCG GCTGCTTCTC ATATTACCAG 
GAGGCCAAGA GCTCCAAGAT CATGGAGTCT TTTAAGAACA TGGTGCCTCA GCAAGCTCTG 
GTGATTCGAG ATGGAGAGAA AATGCAAATC AATGTAAGAG ATGTGGTTTT GGGAGACCTA 
GTAGAGGTGA AGGGAGGAGA CCAAATCCCT GCTGATATCA GGGTTATCTC GGCGCAAGGA 
TGCAAGGTAG ACAATTCCTC CTTGACTGGG GAGTCAGAAC CCCAGTCTCG CTGCCCTGAT 
TGCACTCATG AGAACCCTCT GGAGACACGG AATATCATCT TCTTCTCCAC TAACTGTGTA 
GAAGGAACAG CTCGGGGTAT TGTGATTGCT ACAGGAGACT ACACAGTAAT GGGCCGGATT 
GCTTCCCTGA CATCAGGCTT GCAAATGGGC AAGACCCCGA TTGCCACCGA AATCGAACAC 
TTCATCCATT TGATCACAGC TGTCGCGGTC TTCCTCGGTG TTTCATTTTT TTGGCTCTCG 
ATTATCTTGG GTTACACCTG GCTGGATGCT GTCATTTTCC TTATTGGTAT CATTGTGGCC 
AATGTGCCGG AAGGGCTGCT GGCCACTGTC ACTGTGTGCC TGACGCTGAC CGCCAAGCGC 
ATGGCTCGCA AGAACTGCCT GGTGAAGAAC CTGGAGGCCG TGGAGACGCT GGGCTCCACG 
TCCACCATCT GCTCAGACAA GACGGGCACC CTCACCCAGA ACCGCATGAC AGTGGCGCAT 
TTGTGGTTTG ATAAGACTGT GTATGAGGCC GACACCAGTG AGGAGCAAAC CACGGGGAAA 
ACGTTTCCCA AGTCCTCAGA CACCTGGTTT TACCTGGCTC GAATCGCTGG CCTCTGTAAC 
CGGGCCGACT TTAAACCCCA TCAGGAGTCT GTTCCCATAG CTAAGCGGGC AACAACAGGG 
GATGCTTCCG AGTCAGCTCT CCTCAAATTC ATTGAGCAGT CTTACAACCC CGTGAGCGAA 
ATGAGGCAGA AAAACCCCAA GGTGGCGGAG ATTCCCTTTA ACTCCACCAA CAAGTATCAG 
ATGTCCATCC ACCTTCTGGA AGATAATTCC GAAGCCCACG TGCTGCTAAT GAAAGGTGCC 
CCAGAGAGGA TCTTTGATTT TTGCTCCAGT TTCCTGCTGA ATGGGCAGGA GTACCCTATG 
GATGAGGAAA TGAAGATGGA CTTCCAGAAT GCCTACATAG AACTGGGAGG TCTGGGCGAA 
CGTGTGCTGG GGTTCTGCTT CTTGAACTTG CCTAGCAATT TCTCTAAGGG GTTCCAGTTT 
AATACCGATG AGTTAAATTT TCCCATGGAA AATCTGTGTT TCGCGGGCCT CATCTCCATG 
ATTGATCCTC CTCGGACGGC CGTGCCTGAC GCTGTGAGCA AATGTCGAAG CGCGGGGATC 
AAGGTGATCA TGGTAACGGG GGATCATCCC ATCACAGCCA AAGCTATTGC CAAAAGTGTG 
GGTATCATTT CAGAAGGTAA CGACACGGCG GAGGACATTG CCGCTCGGCT GAATATCCCT 
ATCAGTCAAG TCAATAACAA GTCTGTTAAA GCCATTGTAG TGCACGGCTC AGAACTGAAA 
GACATGGAAT CCCAACAGCT GGACGACATC CTCAAGTCCT ATAAGGAGAT TGTCTTTGCC 
CGGACCTCTC CTCAGCAGAA ACTCATCATT GTGGAGGGGT GTCAGCGACT GGGAGCCATT 
GTGGCAGTGA CTGGTGACGG GGTGAACGAC TCCCCCGCAC TGAAGAAGGC TGACATCGGC 
ATTGCCATGG GCATCACAGG CTCTGATGTC TCTAAACAGG CGGCTGACAT GATCCTTCTT 
GACGACAATT TTGCCTCCAT TGTGACAGGC GTGGAGGAGG GCCGCCTCAT CTTTGACAAC 
CTGAAGAAGT CCATCGCATA CACCCTGACC AGCAACATCC CTGAGATCAC CCCCTTCCTG 
CTGTTCATCA TCTTAAGCAT ACCCTTGCCT CTGGGCACCA TAACCATTCT CTGCATCGAC 
CTGGGCACTG ACATGGTCCC TGCTATCTCC CTGGCTTATG AATCACCTGA AAGTGACATC 
ATGAAAAGGC TTCCACGGAA CCCCAAAACG GACAATCTTG TGAACAACCG TCTTATTGGC 
ATGGCCTATG GGCAGATTGG GATGATACAG GCTCTGGCTG GATTTTTCAC CTATTTTGTG 
ATCCTGGCGG AGAATGGTTT TAAGCCTCTT GATCTTCTGG GTATCCGTCT ATACTGGGAT 
GATACACAAT TAAATGACCT AGAGGACAGC TATGGGCAGC AGTGGACCTA TGAGCAGCGG 
AAGGTGGTGG AATTCACATG CCAAACGGCC TTCTTCATCA GTATCGTGAT TGTGCAGTGG 
GCTGACCTCA TCATCTGCAA GACACGCAGA AATTCACTCT TCAAGCAGGG CATGAAAAAC 
AAAATCCTAA TATTTGGACT CCTTGAGGAG ACAGTCCTGG CAGCTTTCCT GTCCTACGTT 
CCAGGCATGG ATGTGTCCCT GCGGATGTAC CCACTCAAGA TAAACTGGTG GTTCTGCGCA 
CTTCCCTACA GTGTTCTCAT CTTCGTCTAT GATGAAATCA GAAAACTTAT CATCCGGAGA 
CGGCCTGGTG GATGGCTGGA GAAGGAGACG TACTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Daple Coordinates Planar Polarized Microtubule Dynamics in Ependymal Cells and Contributes to Hydrocephalus.
Cell reports20(4)960-972(2017 Jul)
Takagishi M,Sawada M,Ohata S,Asai N,Enomoto A,Takahashi K,Weng L,Ushida K,Ara H,Matsui S,Kaibuchi K,Sawamoto K,Takahashi M

Ouabain interactions with the ?4 isoform of the sodium pump trigger non-classical steroid hormone signaling and integrin expression in spermatogenic cells.
Biochimica et biophysica acta1863(11)2809-2819(2016 11)
Upmanyu N,Dietze R,Kirch U,Scheiner-Bobis G

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Methylation-dependent and independent regulatory regions in the Na,K-ATPase alpha4 (Atp1a4) gene may impact its testis-specific expression.
Gene575(2 Pt 1)339-52(2016 Jan)
Kumar DL,Kumar PL,James PF

Green fluorescence protein driven by the Na,K-ATPase ?4 isoform promoter is expressed only in male germ cells of mouse testis.
Journal of assisted reproduction and genetics29(12)1313-25(2012 Dec)
McDermott JP,S?nchez G,Chennathukuzhi V,Blanco G

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Atp1a4 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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