B9d1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	B9d1
Entrez Gene ID	27078
Full Name	B9 protein domain 1
Synonyms	AW045994,B9,Eppb9
General protein information	Preferred Names B9 protein domain 1 Names B9 domain-containing protein 1 endothelial precursor cells protein B9
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 B2

mRNA and Protein(s)

mRNA	Protein	Name
XM_006533447.3	XP_006533510.1	B9 domain-containing protein 1 isoform X1
XM_006533447.2	XP_006533510.1	B9 domain-containing protein 1 isoform X3
NM_001330781.1	NP_001317710.1	B9 domain-containing protein 1 isoform 3 precursor
NM_001330780.1	NP_001317709.1	B9 domain-containing protein 1 isoform 2 precursor
NM_013717.3	NP_038745.1	B9 domain-containing protein 1 isoform 1 precursor
XM_006533448.3	XP_006533511.1	B9 domain-containing protein 1 isoform X1
XM_006533448.2	XP_006533511.1	B9 domain-containing protein 1 isoform X3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	B9D1	XP_001153772.1
Xenopus tropicalis (tropical clawed frog)	b9d1	NP_001016309.1
Homo sapiens (human)	B9D1	NP_056496.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006071	XP_001237757.1
	B9D1	XP_001100199.1
Mus musculus (house mouse)	B9d1	NP_038745.1
Rattus norvegicus (Norway rat)	B9d1	NP_001099256.1
Rattus norvegicus (Norway rat)	LOC100911746	XP_003750891.1
Danio rerio (zebrafish)	b9d1	NP_001019544.1
Drosophila melanogaster (fruit fly)	CG14870	NP_650470.1
Bos taurus (cattle)	B9D1	NP_001103534.2
Gallus gallus (chicken)	B9D1	XP_004945274.1
Canis lupus familiaris (dog)	B9D1	XP_536657.3
Caenorhabditis elegans (roundworm)	mksr-1	NP_508203.1

Gene Ontology
Bibliography

Related articles in PubMed

Cilia gene mutations cause atrioventricular septal defects by multiple mechanisms.
Burnicka-Turek O, Steimle JD, Huang W, Felker L, Kamp A, Kweon J, Peterson M, Reeves RH, Maslen CL, Gruber PJ, Yang XH, Shendure J, Moskowitz IP
Human molecular genetics25(14)3011-3028(2016 07)

TMEM231, mutated in orofaciodigital and Meckel syndromes, organizes the ciliary transition zone.
Roberson EC, Dowdle WE, Ozanturk A, Garcia-Gonzalo FR, Li C, Halbritter J, Elkhartoufi N, Porath JD, Cope H, Ashley-Koch A, Gregory S, Thomas S, Sayer JA, Saunier S, Otto EA, Katsanis N, Davis EE, Atti?-Bitach T, Hildebrandt F, Leroux MR, Reiter JF
The Journal of cell biology209(1)129-42(2015 Apr)

Bioinformatic analysis of ciliary transition zone proteins reveals insights into the evolution of ciliopathy networks.
Barker AR, Renzaglia KS, Fry K, Dawe HR
BMC genomics15531(2014 Jun)

Mutations in B9D1 and MKS1 cause mild Joubert syndrome: expanding the genetic overlap with the lethal ciliopathy Meckel syndrome.
Romani M, Micalizzi A, Kraoua I, Dotti MT, Cavallin M, Sztriha L, Ruta R, Mancini F, Mazza T, Castellana S, Hanene B, Carluccio MA, Darra F, M?t? A, Zimmermann A, Gouider-Khouja N, Valente EM
Orphanet journal of rare diseases972(2014 May)

A ciliopathy complex at the transition zone protects the cilia as a privileged membrane domain.
Chih B, Liu P, Chinn Y, Chalouni C, Komuves LG, Hass PE, Sandoval W, Peterson AS
Nature cell biology14(1)61-72(2011 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

B9d1 is required for normal Hh signaling, ciliogenesis, and ciliary protein localization. B9d1 and B9d2 are essential components of a B9 protein complex, disruption of which causes Meckel syndrome.
Title: Disruption of a ciliary B9 protein complex causes Meckel syndrome.

The following B9d1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the B9d1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu09828	NM_013717.3 Latest version!	Mus musculus B9 protein domain 1 (B9d1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OMu46397	NM_001330780.1 Latest version!	Mus musculus B9 protein domain 1 (B9d1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu46398	NM_001330781.1 Latest version!	Mus musculus B9 protein domain 1 (B9d1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu46399	XM_006533448.3 Latest version!	Mus musculus B9 protein domain 1 (B9d1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OMu46399	XM_006533447.3 Latest version!	Mus musculus B9 protein domain 1 (B9d1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OMu46399	XM_006533447.2	Mus musculus B9 protein domain 1 (B9d1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu46399	XM_006533448.2	Mus musculus B9 protein domain 1 (B9d1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

B9d1 ( NM_013717.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_038745.1 Mus musculus B9 protein domain 1 (B9d1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu09828
Clone ID Related Accession (Same CDS sequence)	NM_013717.3
Accession Version	NM_013717.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 615bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Mus musculus(house mouse)
Product	B9 domain-containing protein 1 isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK010355.1 and AW121128.1. On Sep 3, 2016 this sequence version replaced NM_013717.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: AK010355.1, AB030483.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGCAG CAAGTCCCAG CGTCTTCCTG CTCATGATCA CCGGGCAGGT AGAGAGCGCA 
CAGTTTCCAG AGTATGATGA CCTCTACTGC AAGTACTGCT TTGTGTATGG CCAGGACTGG 
GCCCCAACAG CGGGTCTGGA GGAGGGGATC TCACAGATAG CATCTAAGAG CCAAGATGTA 
CGGCAAGCAC TGGTGTGGAA CTTCCCCATC GATGTGACCT TTAAAAGTAC CAACCCCTAT 
GGCTGGCCAC AGATTGTACT CAGTGTCTAT GGGCCGGATG TGTTTGGGAA TGATGTCGTC 
CGAGGCTATG GAGCAGTGCA TGTGCCCCTC TCTCCAGGAC GGCACAAAAG GACCATCCCC 
ATGTTTGTGC CAGAGTCTAC ATCTACACTA CAGAAGTTCA CAAGCTGGTT CATGGGACGG 
CGACCCGAGT ACACAGACCC CAAGGTGGTG GCCCAGGGTG AAGGCCGGGA AGTGACTCGT 
GTTCGCTCTC AGGGATTTGT TACCCTCCTC TTCAATGTGG TGACCAAGGA CATGAAGAAG 
CTGGGCTATG ATACTGGGCC TGTGGACACA CAAGGAGTCC TGGGTCCTAG CCTGCCACAG 
GGCAACCCAC AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038745.1
CDS	66..680
Translation	MAAASPSVFLLMITGQVESAQFPEYDDLYCKYCFVYGQDWAPTAGLEEGISQIASKSQDVRQALVWNFPIDVTFKSTNPYGWPQIVLSVYGPDVFGNDVVRGYGAVHVPLSPGRHKRTIPMFVPESTSTLQKFTSWFMGRRPEYTDPKVVAQGEGREVTRVRSQGFVTLLFNVVTKDMKKLGYDTGPVDTQGVLGPSLPQGNPQ

Target ORF information:

RefSeq Version	NM_013717.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus B9 protein domain 1 (B9d1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013717.3

ORF Insert Sequence:

ATGGCTGCAG CAAGTCCCAG CGTCTTCCTG CTCATGATCA CCGGGCAGGT AGAGAGCGCA 
CAGTTTCCAG AGTATGATGA CCTCTACTGC AAGTACTGCT TTGTGTATGG CCAGGACTGG 
GCCCCAACAG CGGGTCTGGA GGAGGGGATC TCACAGATAG CATCTAAGAG CCAAGATGTA 
CGGCAAGCAC TGGTGTGGAA CTTCCCCATC GATGTGACCT TTAAAAGTAC CAACCCCTAT 
GGCTGGCCAC AGATTGTACT CAGTGTCTAT GGGCCGGATG TGTTTGGGAA TGATGTCGTC 
CGAGGCTATG GAGCAGTGCA TGTGCCCCTC TCTCCAGGAC GGCACAAAAG GACCATCCCC 
ATGTTTGTGC CAGAGTCTAC ATCTACACTA CAGAAGTTCA CAAGCTGGTT CATGGGACGG 
CGACCCGAGT ACACAGACCC CAAGGTGGTG GCCCAGGGTG AAGGCCGGGA AGTGACTCGT 
GTTCGCTCTC AGGGATTTGT TACCCTCCTC TTCAATGTGG TGACCAAGGA CATGAAGAAG 
CTGGGCTATG ATACTGGGCC TGTGGACACA CAAGGAGTCC TGGGTCCTAG CCTGCCACAG 
GGCAACCCAC AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

B9d1 ( NM_001330780.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001317709.1 Mus musculus B9 protein domain 1 (B9d1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu46397
Clone ID Related Accession (Same CDS sequence)	NM_001330780.1
Accession Version	NM_001330780.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Mus musculus(house mouse)
Product	B9 domain-containing protein 1 isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK010355.1, BQ044636.1 and AW121128.1. On Sep 3, 2016 this sequence version replaced XM_006533445.2. Transcript Variant: This variant (2) lacks an exon in the coding region, which results in a frameshift and an early stop codon, compared to variant 1. The encoded isoform (2) is shorter and has a distinct C-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BU846799.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849379, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGCAG CAAGTCCCAG CGTCTTCCTG CTCATGATCA CCGGGCAGGT AGAGAGCGCA 
CAGTTTCCAG AGTATGATGA CCTCTACTGC AAGTACTGCT TTGTGTATGG CCAGGACTGG 
GCCCCAACAG CGGGTCTGGA GGAGGGGATC TCACAGATAG CATCTAAGAG CCAAGATGTA 
CGGCAAGCAC TGGTGTGGAA CTTCCCCATC GATGTGACCT TTAAAAGTAC CAACCCCTAT 
GGCTGCACAA AAGGACCATC CCCATGTTTG TGCCAGAGTC TACATCTACA CTACAGAAGT 
TCACAAGCTG GTTCATGGGA CGGCGACCCG AGTACACAGA CCCCAAGGTG GTGGCCCAGG 
GTGAAGGCCG GGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317709.1
CDS	66..443
Translation	MAAASPSVFLLMITGQVESAQFPEYDDLYCKYCFVYGQDWAPTAGLEEGISQIASKSQDVRQALVWNFPIDVTFKSTNPYGCTKGPSPCLCQSLHLHYRSSQAGSWDGDPSTQTPRWWPRVKAGK

Target ORF information:

RefSeq Version	NM_001330780.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus B9 protein domain 1 (B9d1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330780.1

ORF Insert Sequence:

ATGGCTGCAG CAAGTCCCAG CGTCTTCCTG CTCATGATCA CCGGGCAGGT AGAGAGCGCA 
CAGTTTCCAG AGTATGATGA CCTCTACTGC AAGTACTGCT TTGTGTATGG CCAGGACTGG 
GCCCCAACAG CGGGTCTGGA GGAGGGGATC TCACAGATAG CATCTAAGAG CCAAGATGTA 
CGGCAAGCAC TGGTGTGGAA CTTCCCCATC GATGTGACCT TTAAAAGTAC CAACCCCTAT 
GGCTGCACAA AAGGACCATC CCCATGTTTG TGCCAGAGTC TACATCTACA CTACAGAAGT 
TCACAAGCTG GTTCATGGGA CGGCGACCCG AGTACACAGA CCCCAAGGTG GTGGCCCAGG 
GTGAAGGCCG GGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

B9d1 ( NM_001330781.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001317710.1 Mus musculus B9 protein domain 1 (B9d1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu46398
Clone ID Related Accession (Same CDS sequence)	NM_001330781.1
Accession Version	NM_001330781.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Mus musculus(house mouse)
Product	B9 domain-containing protein 1 isoform 3 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK010355.1, CF551490.1 and AW121128.1. On Sep 3, 2016 this sequence version replaced XM_006533446.2. Transcript Variant: This variant (3) lacks an exon in the coding region, which results in a frameshift and an early stop codon, compared to variant 1. The encoded isoform (3) is shorter and has a distinct C-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: CF551490.1, BG077982.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849376 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGCAG CAAGTCCCAG CGTCTTCCTG CTCATGATCA CCGGGCAGGT AGAGAGCGCA 
CAGTTTCCAG AGTATGATGA CCTCTACTGC AAGTACTGCT TTGTGTATGG CCAGGACTGG 
GCCCCAACAG CGGGCCACAG ATTGTACTCA GTGTCTATGG GCCGGATGTG TTTGGGAATG 
ATGTCGTCCG AGGCTATGGA GCAGTGCATG TGCCCCTCTC TCCAGGACGG CACAAAAGGA 
CCATCCCCAT GTTTGTGCCA GAGTCTACAT CTACACTACA GAAGTTCACA AGCTGGTTCA 
TGGGACGGCG ACCCGAGTAC ACAGACCCCA AGGTGGTGGC CCAGGGTGAA GGCCGGGAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317710.1
CDS	66..428
Translation	MAAASPSVFLLMITGQVESAQFPEYDDLYCKYCFVYGQDWAPTAGHRLYSVSMGRMCLGMMSSEAMEQCMCPSLQDGTKGPSPCLCQSLHLHYRSSQAGSWDGDPSTQTPRWWPRVKAGK

Target ORF information:

RefSeq Version	NM_001330781.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus B9 protein domain 1 (B9d1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330781.1

ORF Insert Sequence:

ATGGCTGCAG CAAGTCCCAG CGTCTTCCTG CTCATGATCA CCGGGCAGGT AGAGAGCGCA 
CAGTTTCCAG AGTATGATGA CCTCTACTGC AAGTACTGCT TTGTGTATGG CCAGGACTGG 
GCCCCAACAG CGGGCCACAG ATTGTACTCA GTGTCTATGG GCCGGATGTG TTTGGGAATG 
ATGTCGTCCG AGGCTATGGA GCAGTGCATG TGCCCCTCTC TCCAGGACGG CACAAAAGGA 
CCATCCCCAT GTTTGTGCCA GAGTCTACAT CTACACTACA GAAGTTCACA AGCTGGTTCA 
TGGGACGGCG ACCCGAGTAC ACAGACCCCA AGGTGGTGGC CCAGGGTGAA GGCCGGGAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

B9d1 ( XM_006533447.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006533510.1 Mus musculus B9 protein domain 1 (B9d1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu46399
Clone ID Related Accession (Same CDS sequence)	XM_006533447.3 , XM_006533447.2 , XM_006533448.3 , XM_006533448.2
Accession Version	XM_006533447.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	B9 domain-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006533447.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGACCT CTACTGCAAG TACTGCTTTG TGTATGGCCA GGACTGGGCC CCAACAGCGG 
CACAAAAGGA CCATCCCCAT GTTTGTGCCA GAGTCTACAT CTACACTACA GAAGTTCACA 
AGCTGGTTCA TGGGACGGCG ACCCGAGTAC ACAGACCCCA AGGTGGTGGC CCAGGGTGAA 
GGCCGGGAAG TGACTCGTGT TCGCTCTCAG GGATTTGTTA CCCTCCTCTT CAATGTGGTG 
ACCAAGGACA TGAAGAAGCT GGGCTATGAT ACTGGGCCTG TGGACACACA AGGAGTCCTG 
GGTCCTAGCC TGCCACAGGG CAACCCACAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006533510.1
CDS	33..365
Translation	MMTSTASTALCMARTGPQQRHKRTIPMFVPESTSTLQKFTSWFMGRRPEYTDPKVVAQGEGREVTRVRSQGFVTLLFNVVTKDMKKLGYDTGPVDTQGVLGPSLPQGNPQ

Target ORF information:

RefSeq Version	XM_006533447.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus B9 protein domain 1 (B9d1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006533447.2

ORF Insert Sequence:

ATGATGACCT CTACTGCAAG TACTGCTTTG TGTATGGCCA GGACTGGGCC CCAACAGCGG 
CACAAAAGGA CCATCCCCAT GTTTGTGCCA GAGTCTACAT CTACACTACA GAAGTTCACA 
AGCTGGTTCA TGGGACGGCG ACCCGAGTAC ACAGACCCCA AGGTGGTGGC CCAGGGTGAA 
GGCCGGGAAG TGACTCGTGT TCGCTCTCAG GGATTTGTTA CCCTCCTCTT CAATGTGGTG 
ACCAAGGACA TGAAGAAGCT GGGCTATGAT ACTGGGCCTG TGGACACACA AGGAGTCCTG 
GGTCCTAGCC TGCCACAGGG CAACCCACAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

B9d1 ( XM_006533448.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006533511.1 Mus musculus B9 protein domain 1 (B9d1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu46399
Clone ID Related Accession (Same CDS sequence)	XM_006533447.3 , XM_006533447.2 , XM_006533448.3 , XM_006533448.2
Accession Version	XM_006533448.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	B9 domain-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006533448.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGACCT CTACTGCAAG TACTGCTTTG TGTATGGCCA GGACTGGGCC CCAACAGCGG 
CACAAAAGGA CCATCCCCAT GTTTGTGCCA GAGTCTACAT CTACACTACA GAAGTTCACA 
AGCTGGTTCA TGGGACGGCG ACCCGAGTAC ACAGACCCCA AGGTGGTGGC CCAGGGTGAA 
GGCCGGGAAG TGACTCGTGT TCGCTCTCAG GGATTTGTTA CCCTCCTCTT CAATGTGGTG 
ACCAAGGACA TGAAGAAGCT GGGCTATGAT ACTGGGCCTG TGGACACACA AGGAGTCCTG 
GGTCCTAGCC TGCCACAGGG CAACCCACAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006533511.1
CDS	421..753
Translation	MMTSTASTALCMARTGPQQRHKRTIPMFVPESTSTLQKFTSWFMGRRPEYTDPKVVAQGEGREVTRVRSQGFVTLLFNVVTKDMKKLGYDTGPVDTQGVLGPSLPQGNPQ

Target ORF information:

RefSeq Version	XM_006533448.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus B9 protein domain 1 (B9d1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006533448.2

ORF Insert Sequence:

ATGATGACCT CTACTGCAAG TACTGCTTTG TGTATGGCCA GGACTGGGCC CCAACAGCGG 
CACAAAAGGA CCATCCCCAT GTTTGTGCCA GAGTCTACAT CTACACTACA GAAGTTCACA 
AGCTGGTTCA TGGGACGGCG ACCCGAGTAC ACAGACCCCA AGGTGGTGGC CCAGGGTGAA 
GGCCGGGAAG TGACTCGTGT TCGCTCTCAG GGATTTGTTA CCCTCCTCTT CAATGTGGTG 
ACCAAGGACA TGAAGAAGCT GGGCTATGAT ACTGGGCCTG TGGACACACA AGGAGTCCTG 
GGTCCTAGCC TGCCACAGGG CAACCCACAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

B9d1 ( XM_006533447.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006533510.1 Mus musculus B9 protein domain 1 (B9d1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu46399
Clone ID Related Accession (Same CDS sequence)	XM_006533447.3 , XM_006533447.2 , XM_006533448.3 , XM_006533448.2
Accession Version	XM_006533447.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	B9 domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006533447.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGACCT CTACTGCAAG TACTGCTTTG TGTATGGCCA GGACTGGGCC CCAACAGCGG 
CACAAAAGGA CCATCCCCAT GTTTGTGCCA GAGTCTACAT CTACACTACA GAAGTTCACA 
AGCTGGTTCA TGGGACGGCG ACCCGAGTAC ACAGACCCCA AGGTGGTGGC CCAGGGTGAA 
GGCCGGGAAG TGACTCGTGT TCGCTCTCAG GGATTTGTTA CCCTCCTCTT CAATGTGGTG 
ACCAAGGACA TGAAGAAGCT GGGCTATGAT ACTGGGCCTG TGGACACACA AGGAGTCCTG 
GGTCCTAGCC TGCCACAGGG CAACCCACAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006533510.1
CDS	106..438
Translation	MMTSTASTALCMARTGPQQRHKRTIPMFVPESTSTLQKFTSWFMGRRPEYTDPKVVAQGEGREVTRVRSQGFVTLLFNVVTKDMKKLGYDTGPVDTQGVLGPSLPQGNPQ

Target ORF information:

RefSeq Version	XM_006533447.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus B9 protein domain 1 (B9d1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006533447.3

ORF Insert Sequence:

ATGATGACCT CTACTGCAAG TACTGCTTTG TGTATGGCCA GGACTGGGCC CCAACAGCGG 
CACAAAAGGA CCATCCCCAT GTTTGTGCCA GAGTCTACAT CTACACTACA GAAGTTCACA 
AGCTGGTTCA TGGGACGGCG ACCCGAGTAC ACAGACCCCA AGGTGGTGGC CCAGGGTGAA 
GGCCGGGAAG TGACTCGTGT TCGCTCTCAG GGATTTGTTA CCCTCCTCTT CAATGTGGTG 
ACCAAGGACA TGAAGAAGCT GGGCTATGAT ACTGGGCCTG TGGACACACA AGGAGTCCTG 
GGTCCTAGCC TGCCACAGGG CAACCCACAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

B9d1 ( XM_006533448.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006533511.1 Mus musculus B9 protein domain 1 (B9d1), transcript variant X2, mRNA.

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CloneID	OMu46399
Clone ID Related Accession (Same CDS sequence)	XM_006533447.3 , XM_006533447.2 , XM_006533448.3 , XM_006533448.2
Accession Version	XM_006533448.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	B9 domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006533448.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGACCT CTACTGCAAG TACTGCTTTG TGTATGGCCA GGACTGGGCC CCAACAGCGG 
CACAAAAGGA CCATCCCCAT GTTTGTGCCA GAGTCTACAT CTACACTACA GAAGTTCACA 
AGCTGGTTCA TGGGACGGCG ACCCGAGTAC ACAGACCCCA AGGTGGTGGC CCAGGGTGAA 
GGCCGGGAAG TGACTCGTGT TCGCTCTCAG GGATTTGTTA CCCTCCTCTT CAATGTGGTG 
ACCAAGGACA TGAAGAAGCT GGGCTATGAT ACTGGGCCTG TGGACACACA AGGAGTCCTG 
GGTCCTAGCC TGCCACAGGG CAACCCACAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006533511.1
CDS	397..729
Translation	MMTSTASTALCMARTGPQQRHKRTIPMFVPESTSTLQKFTSWFMGRRPEYTDPKVVAQGEGREVTRVRSQGFVTLLFNVVTKDMKKLGYDTGPVDTQGVLGPSLPQGNPQ

Target ORF information:

RefSeq Version	XM_006533448.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus B9 protein domain 1 (B9d1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006533448.3

ORF Insert Sequence:

ATGATGACCT CTACTGCAAG TACTGCTTTG TGTATGGCCA GGACTGGGCC CCAACAGCGG 
CACAAAAGGA CCATCCCCAT GTTTGTGCCA GAGTCTACAT CTACACTACA GAAGTTCACA 
AGCTGGTTCA TGGGACGGCG ACCCGAGTAC ACAGACCCCA AGGTGGTGGC CCAGGGTGAA 
GGCCGGGAAG TGACTCGTGT TCGCTCTCAG GGATTTGTTA CCCTCCTCTT CAATGTGGTG 
ACCAAGGACA TGAAGAAGCT GGGCTATGAT ACTGGGCCTG TGGACACACA AGGAGTCCTG 
GGTCCTAGCC TGCCACAGGG CAACCCACAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cilia gene mutations cause atrioventricular septal defects by multiple mechanisms.
Human molecular genetics25(14)3011-3028(2016 07)
Burnicka-Turek O,Steimle JD,Huang W,Felker L,Kamp A,Kweon J,Peterson M,Reeves RH,Maslen CL,Gruber PJ,Yang XH,Shendure J,Moskowitz IP

TMEM231, mutated in orofaciodigital and Meckel syndromes, organizes the ciliary transition zone.
The Journal of cell biology209(1)129-42(2015 Apr)
Roberson EC,Dowdle WE,Ozanturk A,Garcia-Gonzalo FR,Li C,Halbritter J,Elkhartoufi N,Porath JD,Cope H,Ashley-Koch A,Gregory S,Thomas S,Sayer JA,Saunier S,Otto EA,Katsanis N,Davis EE,Atti?-Bitach T,Hildebrandt F,Leroux MR,Reiter JF

Bioinformatic analysis of ciliary transition zone proteins reveals insights into the evolution of ciliopathy networks.
BMC genomics15531(2014 Jun)
Barker AR,Renzaglia KS,Fry K,Dawe HR

Mutations in B9D1 and MKS1 cause mild Joubert syndrome: expanding the genetic overlap with the lethal ciliopathy Meckel syndrome.
Orphanet journal of rare diseases972(2014 May)
Romani M,Micalizzi A,Kraoua I,Dotti MT,Cavallin M,Sztriha L,Ruta R,Mancini F,Mazza T,Castellana S,Hanene B,Carluccio MA,Darra F,M?t? A,Zimmermann A,Gouider-Khouja N,Valente EM

A ciliopathy complex at the transition zone protects the cilia as a privileged membrane domain.
Nature cell biology14(1)61-72(2011 Dec)
Chih B,Liu P,Chinn Y,Chalouni C,Komuves LG,Hass PE,Sandoval W,Peterson AS

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B9d1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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