Tcirg1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tcirg1
Entrez Gene ID	27060
Full Name	T cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 protein A3
Synonyms	ATP6N1C,ATP6a3,Atp6i,OC-116,OPTB1,Stv1,TIRC7,Vph1,oc
General protein information	Preferred Names T cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 protein A3 Names V-type proton ATPase 116 kDa subunit a ATPase, H+ transporting, lysosomal I V-ATPase a3 osteosclerotic
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 19 Map Location: 19 A\|19 3.62 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_011248632.1	XP_011246934.1	V-type proton ATPase 116 kDa subunit a isoform X1
NM_016921.3	NP_058617.3	V-type proton ATPase 116 kDa subunit a
NM_001136091.2	NP_001129563.1	V-type proton ATPase 116 kDa subunit a
NM_001167784.1	NP_001161256.1	V-type proton ATPase 116 kDa subunit a

Pathways from BioSystems

Homologies

Homo sapiens (human)	TCIRG1	NP_006010.2
Pan troglodytes (chimpanzee)	TCIRG1	XP_522295.3
Danio rerio (zebrafish)	zgc:55891	NP_998234.1
Danio rerio (zebrafish)	LOC100147923	XP_001922910.4
Xenopus tropicalis (tropical clawed frog)	tcirg1	NP_001016849.1
	TCIRG1	XP_001103418.2
Rattus norvegicus (Norway rat)	Tcirg1	NP_954520.2
Canis lupus familiaris (dog)	TCIRG1	XP_540812.1
Bos taurus (cattle)	TCIRG1	NP_001069102.1
Gallus gallus (chicken)	TCIRG1	NP_990053.1
Danio rerio (zebrafish)	tcirg1	XP_005160329.1
Mus musculus (house mouse)	Tcirg1	NP_001129563.1

Gene Ontology
Bibliography

Related articles in PubMed

Activity-independent targeting of mTOR to lysosomes in primary osteoclasts.
Wang A, Carraro-Lacroix LR, Owen C, Gao B, Corey PN, Tyrrell P, Brumell JH, Voronov I
Scientific reports7(1)3005(2017 Jun)

Oocyte-expressed yes-associated protein is a key activator of the early zygotic genome in mouse.
Yu C, Ji SY, Dang YJ, Sha QQ, Yuan YF, Zhou JJ, Yan LY, Qiao J, Tang F, Fan HY
Cell research26(3)275-87(2016 Mar)

Osteopetrorickets due to Snx10 deficiency in mice results from both failed osteoclast activity and loss of gastric acid-dependent calcium absorption.
Ye L, Morse LR, Zhang L, Sasaki H, Mills JC, Odgren PR, Sibbel G, Stanley JR, Wong G, Zamarioli A, Battaglino RA
PLoS genetics11(3)e1005057(2015 Mar)

The R740S mutation in the V-ATPase a3 subunit results in osteoclast apoptosis and defective early-stage autophagy.
Ochotny N, Voronov I, Owen C, Aubin JE, Manolson MF
Journal of cellular biochemistry114(12)2823-33(2013 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Here we show mice homozygous for R740S (R740S/R740S) of the Vacuolar-type H(+)-ATPases have more severe osteopetrosis and die by postnatal day 14
Title: The R740S mutation in the V-ATPase a3 subunit results in osteoclast apoptosis and defective early-stage autophagy.

These results demonstrate that adeno-associated virus-mediated Atp6i/TIRC7 knockdown in periapical tissues can inhibit endodontic disease development, bone resorption, and inflammation.
Title: RNA interference-mediated silencing of Atp6i prevents both periapical bone erosion and inflammation in the mouse model of endodontic disease.

Luteolin can be effective in reducing bone resorption and that this effect of luteolin may be through disruption of osteoclast V-ATPase a3-d2 interaction.
Title: Luteolin inhibition of V-ATPase a3-d2 interaction decreases osteoclast resorptive activity.

The N termini of a-subunit isoforms are involved in signaling between vacuolar H+-ATPase (V-ATPase) and cytohesin-2
Title: The N termini of a-subunit isoforms are involved in signaling between vacuolar H+-ATPase (V-ATPase) and cytohesin-2.

increased lysosomal pH in V ATPase a3 mutant mice osteoclasts leads to decreased NFATc1 signaling and nuclear translocation, resulting in a cell autonomous impairment of osteoclastogenesis in vitro.
Title: The R740S mutation in the V-ATPase a3 subunit increases lysosomal pH, impairs NFATc1 translocation, and decreases in vitro osteoclastogenesis.

The following Tcirg1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tcirg1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu20568	NM_016921.3 Latest version!	Mus musculus T cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 protein A3 (Tcirg1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu20568	NM_001136091.2 Latest version!	Mus musculus T cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 protein A3 (Tcirg1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu20568	NM_001167784.1 Latest version!	Mus musculus T cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 protein A3 (Tcirg1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu69266	XM_011248632.1 Latest version!	Mus musculus T cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 protein A3 (Tcirg1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tcirg1 ( NM_016921.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_058617.3 Mus musculus T cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 protein A3 (Tcirg1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu20568
Clone ID Related Accession (Same CDS sequence)	NM_016921.3 , NM_001136091.2 , NM_001167784.1
Accession Version	NM_016921.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2505bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Mus musculus(house mouse)
Product	V-type proton ATPase 116 kDa subunit a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC133523.3, AB022322.1 and BE987533.1. On Nov 17, 2009 this sequence version replaced NM_016921.2. Transcript Variant: This variant (1) differs in the 5' UTR compared to variant 3. All three variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC006761.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164137 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCTCTA TGTTCCGGAG TGAAGAGGTG GCCTTGGTCC AGCTCCTGTT GCCCACAGGG 
TCTGCTTACA ACTGTGTGAG CCAGCTTGGT GAGCTGGGCC TCGTGGAGTT CAGAGACCTC 
AACGAATCCG TGAGCGCCTT CCAGAGACGC TTCGTGGTAG ACGTCCGGCG CTGCGAGGAA 
CTGGAGAAGA CGTTTACCTT CTTGCGGGAA GAAGTGCAGC GGGCAGGTCT GACGCTGGCC 
CCACCTGAGG GGACACTGCC AGCACCACCT CCCCGAGACC TGCTGCGCAT CCAGGAGGAA 
ACGGATCGCT TGGCCCAGGA GCTTCGGGAT GTTCGTGGCA ATCAGCAGGC ACTGCGGGCC 
CAGCTGCACC AGCTGCGGCT TCACTCGGCT GTGCTGGGCC AGAGCCACAG CCCGCCGGTG 
GCAGCTGACC ATACTGAAGG ACCCTTCTCT GAGACAACAC CCCTGCTTCC AGGCACCCGG 
GGGCCACATT CAGACCTGAA GGTCAATTTT GTGGCAGGTG CTGTGGAGCC CTACAAGGCT 
GCGGCCCTGG AGCGCCTGCT GTGGAGAGCT TGCCGCGGCT TCCTCATTGC CAGCTTTCGG 
GAGACTGAGG GGCAGCTGGA GGACCCAGTG ACGGGTGAGC CTGCAACTTG GATGACCTTT 
GTCATCTCCT ACTGGGGTGA GCAGATCGGA CAGAAGATCC GAAAGATCAC AGACTGCTTC 
CACTGTCATG TCTTCCCATA CCTGGAGCAG GAGGAAGCCC GGTTCAGGAC CTTGCAGCAG 
CTGCAGCAGC AAAGCCAGGA GCTTCAGGAA GTCCTGGGGG AGACAGACCG GTTCCTGAGC 
CAGGTGTTGG GCCGGGTGCA GCAGCTGCTG CCCCCATGGC AGGTGCAGAT CCACAAGATG 
AAGGCAGTGT ACCTGACCCT CAACCAGTGC AGTGTGAACA CCACACACAA GTGCCTCATC 
GCGGAGGTCT GGTGTGCCGC GAGGGACCTG CCCACTGTGC AGCAGGCGCT GCAGAGCGGC 
TCAAGTGAGG AAGGAGTGAG TGCTGTCGCT CACCGCATCC CCTGCCAGGA CATGCCTCCA 
ACCCTCATCA GGACCAACCG CTTCACCTCC AGCTTCCAGG GCATTGTGGA TGCCTATGGT 
GTGGGCCGCT ACAGGGAAGT TAACCCTGCT CCCTACACCA TCATCACATT CCCCTTTCTC 
TTCGCTGTGA TGTTTGGCGA TGTGGGGCAC GGACTGCTCA TGTTTCTCTT TGCCCTGGCC 
ATGGTCCTCA CTGAAAACCG TCCAGCTGTG AAGGCTGCAC AAAACGAGAT CTGGCAGACC 
TTCTTTGGGG GTCGATACCT ACTCCTGCTC ATGGGTCTGT TCTCCGTCTA CACTGGCTTC 
ATCTACAATG AGTGCTTCAG CCGAGCCACC ACTATTTTCC CCTCAGGTTG GAGTGTGGCT 
GCCATGGCCA ACCAGTCAGG CTGGAGTGAC GAGTATCTGT CCCAGCACTC CATGCTCACC 
CTGAACCCTA ACATCACTGG TGTCTTCCTT GGACCATATC CCTTTGGCAT TGACCCGATC 
TGGAGCCTGG CCACGAACCA CCTGAGCTTT CTCAACTCCT TCAAGATGAA GATGTCTGTC 
ATCCTTGGGG TCACGCACAT GGCCTTTGGG GTGTTCCTCA GCATCTTCAA CCACGTGCAC 
TTTGGCCAGG CCCACCGGCT ACTGCTGGAG ACCTTGCCTG AGCTCATCTT CCTGCTGGGT 
CTCTTTGGCT ACCTTGTGTT CCTCATTGTC TACAAGTGGG TGAATGTCTC AGCTGCTAGC 
GCCTCCTCGG CCCCCAGTAT CCTCATTCAC TTCATCAATA TGTTCCTCTT CTCTCAAAAC 
CCCACCAATC ATCTGCTCTT CCATGGGCAG GAAGTGGTAC AGTACGTGCT GGTGGTCCTG 
GCTTTGGCTA CCGTTCCTAT CCTGTTGCTG GGCACACCCT TGTACCTGCT GCGCCAGCAC 
CGCCACAGAA GAAACACTCA GAGAAGGCCA GCAGGCCAGC AGGATGAGGA CACGGACAAG 
CTTCTGGCCT CCCCTGATGC ATCCACCTTG GAGAACAGCT GGAGCCCTGA TGAGGAGAAG 
GCTGGGAGCC CAGGGGATGA AGAAACTGAG TTTGTCCCTT CTGAGATCTT CATGCACCAA 
GCAATCCACA CCATTGAGTT CTGCCTGGGC TGCATCTCCA ACACAGCCTC CTACTTGCGT 
CTCTGGGCCC TGAGCCTGGC CCATGCCCAG CTGTCTGAGG TCCTGTGGGC CATGGTGATG 
CGCATAGGCC TGGGCATGGG CCGAGAGATC GGCGTGGCAG CTGTGGTGCT GGTCCCTGTG 
TTTGCTGCCT TTGCTGTGTT GACTGTGGCC ATCCTGTTAG TGATGGAGGG GCTCTCAGCC 
TTCCTGCATG CCCTGCGGTT GCACTGGGTG GAGTTCCAGA ACAAGTTCTA TTCAGGCACC 
GGCTACAAGC TCAGCCCCTT CACCTTCACT GTGGACAGTG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058617.3
CDS	161..2665
Translation	MGSMFRSEEVALVQLLLPTGSAYNCVSQLGELGLVEFRDLNESVSAFQRRFVVDVRRCEELEKTFTFLREEVQRAGLTLAPPEGTLPAPPPRDLLRIQEETDRLAQELRDVRGNQQALRAQLHQLRLHSAVLGQSHSPPVAADHTEGPFSETTPLLPGTRGPHSDLKVNFVAGAVEPYKAAALERLLWRACRGFLIASFRETEGQLEDPVTGEPATWMTFVISYWGEQIGQKIRKITDCFHCHVFPYLEQEEARFRTLQQLQQQSQELQEVLGETDRFLSQVLGRVQQLLPPWQVQIHKMKAVYLTLNQCSVNTTHKCLIAEVWCAARDLPTVQQALQSGSSEEGVSAVAHRIPCQDMPPTLIRTNRFTSSFQGIVDAYGVGRYREVNPAPYTIITFPFLFAVMFGDVGHGLLMFLFALAMVLTENRPAVKAAQNEIWQTFFGGRYLLLLMGLFSVYTGFIYNECFSRATTIFPSGWSVAAMANQSGWSDEYLSQHSMLTLNPNITGVFLGPYPFGIDPIWSLATNHLSFLNSFKMKMSVILGVTHMAFGVFLSIFNHVHFGQAHRLLLETLPELIFLLGLFGYLVFLIVYKWVNVSAASASSAPSILIHFINMFLFSQNPTNHLLFHGQEVVQYVLVVLALATVPILLLGTPLYLLRQHRHRRNTQRRPAGQQDEDTDKLLASPDASTLENSWSPDEEKAGSPGDEETEFVPSEIFMHQAIHTIEFCLGCISNTASYLRLWALSLAHAQLSEVLWAMVMRIGLGMGREIGVAAVVLVPVFAAFAVLTVAILLVMEGLSAFLHALRLHWVEFQNKFYSGTGYKLSPFTFTVDSD

Target ORF information:

RefSeq Version	NM_016921.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus T cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 protein A3 (Tcirg1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016921.3

ORF Insert Sequence:

ATGGGCTCTA TGTTCCGGAG TGAAGAGGTG GCCTTGGTCC AGCTCCTGTT GCCCACAGGG 
TCTGCTTACA ACTGTGTGAG CCAGCTTGGT GAGCTGGGCC TCGTGGAGTT CAGAGACCTC 
AACGAATCCG TGAGCGCCTT CCAGAGACGC TTCGTGGTAG ACGTCCGGCG CTGCGAGGAA 
CTGGAGAAGA CGTTTACCTT CTTGCGGGAA GAAGTGCAGC GGGCAGGTCT GACGCTGGCC 
CCACCTGAGG GGACACTGCC AGCACCACCT CCCCGAGACC TGCTGCGCAT CCAGGAGGAA 
ACGGATCGCT TGGCCCAGGA GCTTCGGGAT GTTCGTGGCA ATCAGCAGGC ACTGCGGGCC 
CAGCTGCACC AGCTGCGGCT TCACTCGGCT GTGCTGGGCC AGAGCCACAG CCCGCCGGTG 
GCAGCTGACC ATACTGAAGG ACCCTTCTCT GAGACAACAC CCCTGCTTCC AGGCACCCGG 
GGGCCACATT CAGACCTGAA GGTCAATTTT GTGGCAGGTG CTGTGGAGCC CTACAAGGCT 
GCGGCCCTGG AGCGCCTGCT GTGGAGAGCT TGCCGCGGCT TCCTCATTGC CAGCTTTCGG 
GAGACTGAGG GGCAGCTGGA GGACCCAGTG ACGGGTGAGC CTGCAACTTG GATGACCTTT 
GTCATCTCCT ACTGGGGTGA GCAGATCGGA CAGAAGATCC GAAAGATCAC AGACTGCTTC 
CACTGTCATG TCTTCCCATA CCTGGAGCAG GAGGAAGCCC GGTTCAGGAC CTTGCAGCAG 
CTGCAGCAGC AAAGCCAGGA GCTTCAGGAA GTCCTGGGGG AGACAGACCG GTTCCTGAGC 
CAGGTGTTGG GCCGGGTGCA GCAGCTGCTG CCCCCATGGC AGGTGCAGAT CCACAAGATG 
AAGGCAGTGT ACCTGACCCT CAACCAGTGC AGTGTGAACA CCACACACAA GTGCCTCATC 
GCGGAGGTCT GGTGTGCCGC GAGGGACCTG CCCACTGTGC AGCAGGCGCT GCAGAGCGGC 
TCAAGTGAGG AAGGAGTGAG TGCTGTCGCT CACCGCATCC CCTGCCAGGA CATGCCTCCA 
ACCCTCATCA GGACCAACCG CTTCACCTCC AGCTTCCAGG GCATTGTGGA TGCCTATGGT 
GTGGGCCGCT ACAGGGAAGT TAACCCTGCT CCCTACACCA TCATCACATT CCCCTTTCTC 
TTCGCTGTGA TGTTTGGCGA TGTGGGGCAC GGACTGCTCA TGTTTCTCTT TGCCCTGGCC 
ATGGTCCTCA CTGAAAACCG TCCAGCTGTG AAGGCTGCAC AAAACGAGAT CTGGCAGACC 
TTCTTTGGGG GTCGATACCT ACTCCTGCTC ATGGGTCTGT TCTCCGTCTA CACTGGCTTC 
ATCTACAATG AGTGCTTCAG CCGAGCCACC ACTATTTTCC CCTCAGGTTG GAGTGTGGCT 
GCCATGGCCA ACCAGTCAGG CTGGAGTGAC GAGTATCTGT CCCAGCACTC CATGCTCACC 
CTGAACCCTA ACATCACTGG TGTCTTCCTT GGACCATATC CCTTTGGCAT TGACCCGATC 
TGGAGCCTGG CCACGAACCA CCTGAGCTTT CTCAACTCCT TCAAGATGAA GATGTCTGTC 
ATCCTTGGGG TCACGCACAT GGCCTTTGGG GTGTTCCTCA GCATCTTCAA CCACGTGCAC 
TTTGGCCAGG CCCACCGGCT ACTGCTGGAG ACCTTGCCTG AGCTCATCTT CCTGCTGGGT 
CTCTTTGGCT ACCTTGTGTT CCTCATTGTC TACAAGTGGG TGAATGTCTC AGCTGCTAGC 
GCCTCCTCGG CCCCCAGTAT CCTCATTCAC TTCATCAATA TGTTCCTCTT CTCTCAAAAC 
CCCACCAATC ATCTGCTCTT CCATGGGCAG GAAGTGGTAC AGTACGTGCT GGTGGTCCTG 
GCTTTGGCTA CCGTTCCTAT CCTGTTGCTG GGCACACCCT TGTACCTGCT GCGCCAGCAC 
CGCCACAGAA GAAACACTCA GAGAAGGCCA GCAGGCCAGC AGGATGAGGA CACGGACAAG 
CTTCTGGCCT CCCCTGATGC ATCCACCTTG GAGAACAGCT GGAGCCCTGA TGAGGAGAAG 
GCTGGGAGCC CAGGGGATGA AGAAACTGAG TTTGTCCCTT CTGAGATCTT CATGCACCAA 
GCAATCCACA CCATTGAGTT CTGCCTGGGC TGCATCTCCA ACACAGCCTC CTACTTGCGT 
CTCTGGGCCC TGAGCCTGGC CCATGCCCAG CTGTCTGAGG TCCTGTGGGC CATGGTGATG 
CGCATAGGCC TGGGCATGGG CCGAGAGATC GGCGTGGCAG CTGTGGTGCT GGTCCCTGTG 
TTTGCTGCCT TTGCTGTGTT GACTGTGGCC ATCCTGTTAG TGATGGAGGG GCTCTCAGCC 
TTCCTGCATG CCCTGCGGTT GCACTGGGTG GAGTTCCAGA ACAAGTTCTA TTCAGGCACC 
GGCTACAAGC TCAGCCCCTT CACCTTCACT GTGGACAGTG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tcirg1 ( NM_001136091.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001129563.1 Mus musculus T cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 protein A3 (Tcirg1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu20568
Clone ID Related Accession (Same CDS sequence)	NM_016921.3 , NM_001136091.2 , NM_001167784.1
Accession Version	NM_001136091.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2505bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Mus musculus(house mouse)
Product	V-type proton ATPase 116 kDa subunit a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ057829.1, AB022322.1 and BE987533.1. On Nov 17, 2009 this sequence version replaced NM_001136091.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 3. All three variants encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF218253.1, BC085234.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164137 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCTCTA TGTTCCGGAG TGAAGAGGTG GCCTTGGTCC AGCTCCTGTT GCCCACAGGG 
TCTGCTTACA ACTGTGTGAG CCAGCTTGGT GAGCTGGGCC TCGTGGAGTT CAGAGACCTC 
AACGAATCCG TGAGCGCCTT CCAGAGACGC TTCGTGGTAG ACGTCCGGCG CTGCGAGGAA 
CTGGAGAAGA CGTTTACCTT CTTGCGGGAA GAAGTGCAGC GGGCAGGTCT GACGCTGGCC 
CCACCTGAGG GGACACTGCC AGCACCACCT CCCCGAGACC TGCTGCGCAT CCAGGAGGAA 
ACGGATCGCT TGGCCCAGGA GCTTCGGGAT GTTCGTGGCA ATCAGCAGGC ACTGCGGGCC 
CAGCTGCACC AGCTGCGGCT TCACTCGGCT GTGCTGGGCC AGAGCCACAG CCCGCCGGTG 
GCAGCTGACC ATACTGAAGG ACCCTTCTCT GAGACAACAC CCCTGCTTCC AGGCACCCGG 
GGGCCACATT CAGACCTGAA GGTCAATTTT GTGGCAGGTG CTGTGGAGCC CTACAAGGCT 
GCGGCCCTGG AGCGCCTGCT GTGGAGAGCT TGCCGCGGCT TCCTCATTGC CAGCTTTCGG 
GAGACTGAGG GGCAGCTGGA GGACCCAGTG ACGGGTGAGC CTGCAACTTG GATGACCTTT 
GTCATCTCCT ACTGGGGTGA GCAGATCGGA CAGAAGATCC GAAAGATCAC AGACTGCTTC 
CACTGTCATG TCTTCCCATA CCTGGAGCAG GAGGAAGCCC GGTTCAGGAC CTTGCAGCAG 
CTGCAGCAGC AAAGCCAGGA GCTTCAGGAA GTCCTGGGGG AGACAGACCG GTTCCTGAGC 
CAGGTGTTGG GCCGGGTGCA GCAGCTGCTG CCCCCATGGC AGGTGCAGAT CCACAAGATG 
AAGGCAGTGT ACCTGACCCT CAACCAGTGC AGTGTGAACA CCACACACAA GTGCCTCATC 
GCGGAGGTCT GGTGTGCCGC GAGGGACCTG CCCACTGTGC AGCAGGCGCT GCAGAGCGGC 
TCAAGTGAGG AAGGAGTGAG TGCTGTCGCT CACCGCATCC CCTGCCAGGA CATGCCTCCA 
ACCCTCATCA GGACCAACCG CTTCACCTCC AGCTTCCAGG GCATTGTGGA TGCCTATGGT 
GTGGGCCGCT ACAGGGAAGT TAACCCTGCT CCCTACACCA TCATCACATT CCCCTTTCTC 
TTCGCTGTGA TGTTTGGCGA TGTGGGGCAC GGACTGCTCA TGTTTCTCTT TGCCCTGGCC 
ATGGTCCTCA CTGAAAACCG TCCAGCTGTG AAGGCTGCAC AAAACGAGAT CTGGCAGACC 
TTCTTTGGGG GTCGATACCT ACTCCTGCTC ATGGGTCTGT TCTCCGTCTA CACTGGCTTC 
ATCTACAATG AGTGCTTCAG CCGAGCCACC ACTATTTTCC CCTCAGGTTG GAGTGTGGCT 
GCCATGGCCA ACCAGTCAGG CTGGAGTGAC GAGTATCTGT CCCAGCACTC CATGCTCACC 
CTGAACCCTA ACATCACTGG TGTCTTCCTT GGACCATATC CCTTTGGCAT TGACCCGATC 
TGGAGCCTGG CCACGAACCA CCTGAGCTTT CTCAACTCCT TCAAGATGAA GATGTCTGTC 
ATCCTTGGGG TCACGCACAT GGCCTTTGGG GTGTTCCTCA GCATCTTCAA CCACGTGCAC 
TTTGGCCAGG CCCACCGGCT ACTGCTGGAG ACCTTGCCTG AGCTCATCTT CCTGCTGGGT 
CTCTTTGGCT ACCTTGTGTT CCTCATTGTC TACAAGTGGG TGAATGTCTC AGCTGCTAGC 
GCCTCCTCGG CCCCCAGTAT CCTCATTCAC TTCATCAATA TGTTCCTCTT CTCTCAAAAC 
CCCACCAATC ATCTGCTCTT CCATGGGCAG GAAGTGGTAC AGTACGTGCT GGTGGTCCTG 
GCTTTGGCTA CCGTTCCTAT CCTGTTGCTG GGCACACCCT TGTACCTGCT GCGCCAGCAC 
CGCCACAGAA GAAACACTCA GAGAAGGCCA GCAGGCCAGC AGGATGAGGA CACGGACAAG 
CTTCTGGCCT CCCCTGATGC ATCCACCTTG GAGAACAGCT GGAGCCCTGA TGAGGAGAAG 
GCTGGGAGCC CAGGGGATGA AGAAACTGAG TTTGTCCCTT CTGAGATCTT CATGCACCAA 
GCAATCCACA CCATTGAGTT CTGCCTGGGC TGCATCTCCA ACACAGCCTC CTACTTGCGT 
CTCTGGGCCC TGAGCCTGGC CCATGCCCAG CTGTCTGAGG TCCTGTGGGC CATGGTGATG 
CGCATAGGCC TGGGCATGGG CCGAGAGATC GGCGTGGCAG CTGTGGTGCT GGTCCCTGTG 
TTTGCTGCCT TTGCTGTGTT GACTGTGGCC ATCCTGTTAG TGATGGAGGG GCTCTCAGCC 
TTCCTGCATG CCCTGCGGTT GCACTGGGTG GAGTTCCAGA ACAAGTTCTA TTCAGGCACC 
GGCTACAAGC TCAGCCCCTT CACCTTCACT GTGGACAGTG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001129563.1
CDS	120..2624
Translation	MGSMFRSEEVALVQLLLPTGSAYNCVSQLGELGLVEFRDLNESVSAFQRRFVVDVRRCEELEKTFTFLREEVQRAGLTLAPPEGTLPAPPPRDLLRIQEETDRLAQELRDVRGNQQALRAQLHQLRLHSAVLGQSHSPPVAADHTEGPFSETTPLLPGTRGPHSDLKVNFVAGAVEPYKAAALERLLWRACRGFLIASFRETEGQLEDPVTGEPATWMTFVISYWGEQIGQKIRKITDCFHCHVFPYLEQEEARFRTLQQLQQQSQELQEVLGETDRFLSQVLGRVQQLLPPWQVQIHKMKAVYLTLNQCSVNTTHKCLIAEVWCAARDLPTVQQALQSGSSEEGVSAVAHRIPCQDMPPTLIRTNRFTSSFQGIVDAYGVGRYREVNPAPYTIITFPFLFAVMFGDVGHGLLMFLFALAMVLTENRPAVKAAQNEIWQTFFGGRYLLLLMGLFSVYTGFIYNECFSRATTIFPSGWSVAAMANQSGWSDEYLSQHSMLTLNPNITGVFLGPYPFGIDPIWSLATNHLSFLNSFKMKMSVILGVTHMAFGVFLSIFNHVHFGQAHRLLLETLPELIFLLGLFGYLVFLIVYKWVNVSAASASSAPSILIHFINMFLFSQNPTNHLLFHGQEVVQYVLVVLALATVPILLLGTPLYLLRQHRHRRNTQRRPAGQQDEDTDKLLASPDASTLENSWSPDEEKAGSPGDEETEFVPSEIFMHQAIHTIEFCLGCISNTASYLRLWALSLAHAQLSEVLWAMVMRIGLGMGREIGVAAVVLVPVFAAFAVLTVAILLVMEGLSAFLHALRLHWVEFQNKFYSGTGYKLSPFTFTVDSD

Target ORF information:

RefSeq Version	NM_001136091.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus T cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 protein A3 (Tcirg1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001136091.2

ORF Insert Sequence:

ATGGGCTCTA TGTTCCGGAG TGAAGAGGTG GCCTTGGTCC AGCTCCTGTT GCCCACAGGG 
TCTGCTTACA ACTGTGTGAG CCAGCTTGGT GAGCTGGGCC TCGTGGAGTT CAGAGACCTC 
AACGAATCCG TGAGCGCCTT CCAGAGACGC TTCGTGGTAG ACGTCCGGCG CTGCGAGGAA 
CTGGAGAAGA CGTTTACCTT CTTGCGGGAA GAAGTGCAGC GGGCAGGTCT GACGCTGGCC 
CCACCTGAGG GGACACTGCC AGCACCACCT CCCCGAGACC TGCTGCGCAT CCAGGAGGAA 
ACGGATCGCT TGGCCCAGGA GCTTCGGGAT GTTCGTGGCA ATCAGCAGGC ACTGCGGGCC 
CAGCTGCACC AGCTGCGGCT TCACTCGGCT GTGCTGGGCC AGAGCCACAG CCCGCCGGTG 
GCAGCTGACC ATACTGAAGG ACCCTTCTCT GAGACAACAC CCCTGCTTCC AGGCACCCGG 
GGGCCACATT CAGACCTGAA GGTCAATTTT GTGGCAGGTG CTGTGGAGCC CTACAAGGCT 
GCGGCCCTGG AGCGCCTGCT GTGGAGAGCT TGCCGCGGCT TCCTCATTGC CAGCTTTCGG 
GAGACTGAGG GGCAGCTGGA GGACCCAGTG ACGGGTGAGC CTGCAACTTG GATGACCTTT 
GTCATCTCCT ACTGGGGTGA GCAGATCGGA CAGAAGATCC GAAAGATCAC AGACTGCTTC 
CACTGTCATG TCTTCCCATA CCTGGAGCAG GAGGAAGCCC GGTTCAGGAC CTTGCAGCAG 
CTGCAGCAGC AAAGCCAGGA GCTTCAGGAA GTCCTGGGGG AGACAGACCG GTTCCTGAGC 
CAGGTGTTGG GCCGGGTGCA GCAGCTGCTG CCCCCATGGC AGGTGCAGAT CCACAAGATG 
AAGGCAGTGT ACCTGACCCT CAACCAGTGC AGTGTGAACA CCACACACAA GTGCCTCATC 
GCGGAGGTCT GGTGTGCCGC GAGGGACCTG CCCACTGTGC AGCAGGCGCT GCAGAGCGGC 
TCAAGTGAGG AAGGAGTGAG TGCTGTCGCT CACCGCATCC CCTGCCAGGA CATGCCTCCA 
ACCCTCATCA GGACCAACCG CTTCACCTCC AGCTTCCAGG GCATTGTGGA TGCCTATGGT 
GTGGGCCGCT ACAGGGAAGT TAACCCTGCT CCCTACACCA TCATCACATT CCCCTTTCTC 
TTCGCTGTGA TGTTTGGCGA TGTGGGGCAC GGACTGCTCA TGTTTCTCTT TGCCCTGGCC 
ATGGTCCTCA CTGAAAACCG TCCAGCTGTG AAGGCTGCAC AAAACGAGAT CTGGCAGACC 
TTCTTTGGGG GTCGATACCT ACTCCTGCTC ATGGGTCTGT TCTCCGTCTA CACTGGCTTC 
ATCTACAATG AGTGCTTCAG CCGAGCCACC ACTATTTTCC CCTCAGGTTG GAGTGTGGCT 
GCCATGGCCA ACCAGTCAGG CTGGAGTGAC GAGTATCTGT CCCAGCACTC CATGCTCACC 
CTGAACCCTA ACATCACTGG TGTCTTCCTT GGACCATATC CCTTTGGCAT TGACCCGATC 
TGGAGCCTGG CCACGAACCA CCTGAGCTTT CTCAACTCCT TCAAGATGAA GATGTCTGTC 
ATCCTTGGGG TCACGCACAT GGCCTTTGGG GTGTTCCTCA GCATCTTCAA CCACGTGCAC 
TTTGGCCAGG CCCACCGGCT ACTGCTGGAG ACCTTGCCTG AGCTCATCTT CCTGCTGGGT 
CTCTTTGGCT ACCTTGTGTT CCTCATTGTC TACAAGTGGG TGAATGTCTC AGCTGCTAGC 
GCCTCCTCGG CCCCCAGTAT CCTCATTCAC TTCATCAATA TGTTCCTCTT CTCTCAAAAC 
CCCACCAATC ATCTGCTCTT CCATGGGCAG GAAGTGGTAC AGTACGTGCT GGTGGTCCTG 
GCTTTGGCTA CCGTTCCTAT CCTGTTGCTG GGCACACCCT TGTACCTGCT GCGCCAGCAC 
CGCCACAGAA GAAACACTCA GAGAAGGCCA GCAGGCCAGC AGGATGAGGA CACGGACAAG 
CTTCTGGCCT CCCCTGATGC ATCCACCTTG GAGAACAGCT GGAGCCCTGA TGAGGAGAAG 
GCTGGGAGCC CAGGGGATGA AGAAACTGAG TTTGTCCCTT CTGAGATCTT CATGCACCAA 
GCAATCCACA CCATTGAGTT CTGCCTGGGC TGCATCTCCA ACACAGCCTC CTACTTGCGT 
CTCTGGGCCC TGAGCCTGGC CCATGCCCAG CTGTCTGAGG TCCTGTGGGC CATGGTGATG 
CGCATAGGCC TGGGCATGGG CCGAGAGATC GGCGTGGCAG CTGTGGTGCT GGTCCCTGTG 
TTTGCTGCCT TTGCTGTGTT GACTGTGGCC ATCCTGTTAG TGATGGAGGG GCTCTCAGCC 
TTCCTGCATG CCCTGCGGTT GCACTGGGTG GAGTTCCAGA ACAAGTTCTA TTCAGGCACC 
GGCTACAAGC TCAGCCCCTT CACCTTCACT GTGGACAGTG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tcirg1 ( NM_001167784.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001161256.1 Mus musculus T cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 protein A3 (Tcirg1), transcript variant 3, mRNA.

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CloneID	OMu20568
Clone ID Related Accession (Same CDS sequence)	NM_016921.3 , NM_001136091.2 , NM_001167784.1
Accession Version	NM_001167784.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2505bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Mus musculus(house mouse)
Product	V-type proton ATPase 116 kDa subunit a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC133523.3, AB022322.1 and BE987533.1. Transcript Variant: This variant (3) represents the longest transcript. All three variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK155140.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164137 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCTCTA TGTTCCGGAG TGAAGAGGTG GCCTTGGTCC AGCTCCTGTT GCCCACAGGG 
TCTGCTTACA ACTGTGTGAG CCAGCTTGGT GAGCTGGGCC TCGTGGAGTT CAGAGACCTC 
AACGAATCCG TGAGCGCCTT CCAGAGACGC TTCGTGGTAG ACGTCCGGCG CTGCGAGGAA 
CTGGAGAAGA CGTTTACCTT CTTGCGGGAA GAAGTGCAGC GGGCAGGTCT GACGCTGGCC 
CCACCTGAGG GGACACTGCC AGCACCACCT CCCCGAGACC TGCTGCGCAT CCAGGAGGAA 
ACGGATCGCT TGGCCCAGGA GCTTCGGGAT GTTCGTGGCA ATCAGCAGGC ACTGCGGGCC 
CAGCTGCACC AGCTGCGGCT TCACTCGGCT GTGCTGGGCC AGAGCCACAG CCCGCCGGTG 
GCAGCTGACC ATACTGAAGG ACCCTTCTCT GAGACAACAC CCCTGCTTCC AGGCACCCGG 
GGGCCACATT CAGACCTGAA GGTCAATTTT GTGGCAGGTG CTGTGGAGCC CTACAAGGCT 
GCGGCCCTGG AGCGCCTGCT GTGGAGAGCT TGCCGCGGCT TCCTCATTGC CAGCTTTCGG 
GAGACTGAGG GGCAGCTGGA GGACCCAGTG ACGGGTGAGC CTGCAACTTG GATGACCTTT 
GTCATCTCCT ACTGGGGTGA GCAGATCGGA CAGAAGATCC GAAAGATCAC AGACTGCTTC 
CACTGTCATG TCTTCCCATA CCTGGAGCAG GAGGAAGCCC GGTTCAGGAC CTTGCAGCAG 
CTGCAGCAGC AAAGCCAGGA GCTTCAGGAA GTCCTGGGGG AGACAGACCG GTTCCTGAGC 
CAGGTGTTGG GCCGGGTGCA GCAGCTGCTG CCCCCATGGC AGGTGCAGAT CCACAAGATG 
AAGGCAGTGT ACCTGACCCT CAACCAGTGC AGTGTGAACA CCACACACAA GTGCCTCATC 
GCGGAGGTCT GGTGTGCCGC GAGGGACCTG CCCACTGTGC AGCAGGCGCT GCAGAGCGGC 
TCAAGTGAGG AAGGAGTGAG TGCTGTCGCT CACCGCATCC CCTGCCAGGA CATGCCTCCA 
ACCCTCATCA GGACCAACCG CTTCACCTCC AGCTTCCAGG GCATTGTGGA TGCCTATGGT 
GTGGGCCGCT ACAGGGAAGT TAACCCTGCT CCCTACACCA TCATCACATT CCCCTTTCTC 
TTCGCTGTGA TGTTTGGCGA TGTGGGGCAC GGACTGCTCA TGTTTCTCTT TGCCCTGGCC 
ATGGTCCTCA CTGAAAACCG TCCAGCTGTG AAGGCTGCAC AAAACGAGAT CTGGCAGACC 
TTCTTTGGGG GTCGATACCT ACTCCTGCTC ATGGGTCTGT TCTCCGTCTA CACTGGCTTC 
ATCTACAATG AGTGCTTCAG CCGAGCCACC ACTATTTTCC CCTCAGGTTG GAGTGTGGCT 
GCCATGGCCA ACCAGTCAGG CTGGAGTGAC GAGTATCTGT CCCAGCACTC CATGCTCACC 
CTGAACCCTA ACATCACTGG TGTCTTCCTT GGACCATATC CCTTTGGCAT TGACCCGATC 
TGGAGCCTGG CCACGAACCA CCTGAGCTTT CTCAACTCCT TCAAGATGAA GATGTCTGTC 
ATCCTTGGGG TCACGCACAT GGCCTTTGGG GTGTTCCTCA GCATCTTCAA CCACGTGCAC 
TTTGGCCAGG CCCACCGGCT ACTGCTGGAG ACCTTGCCTG AGCTCATCTT CCTGCTGGGT 
CTCTTTGGCT ACCTTGTGTT CCTCATTGTC TACAAGTGGG TGAATGTCTC AGCTGCTAGC 
GCCTCCTCGG CCCCCAGTAT CCTCATTCAC TTCATCAATA TGTTCCTCTT CTCTCAAAAC 
CCCACCAATC ATCTGCTCTT CCATGGGCAG GAAGTGGTAC AGTACGTGCT GGTGGTCCTG 
GCTTTGGCTA CCGTTCCTAT CCTGTTGCTG GGCACACCCT TGTACCTGCT GCGCCAGCAC 
CGCCACAGAA GAAACACTCA GAGAAGGCCA GCAGGCCAGC AGGATGAGGA CACGGACAAG 
CTTCTGGCCT CCCCTGATGC ATCCACCTTG GAGAACAGCT GGAGCCCTGA TGAGGAGAAG 
GCTGGGAGCC CAGGGGATGA AGAAACTGAG TTTGTCCCTT CTGAGATCTT CATGCACCAA 
GCAATCCACA CCATTGAGTT CTGCCTGGGC TGCATCTCCA ACACAGCCTC CTACTTGCGT 
CTCTGGGCCC TGAGCCTGGC CCATGCCCAG CTGTCTGAGG TCCTGTGGGC CATGGTGATG 
CGCATAGGCC TGGGCATGGG CCGAGAGATC GGCGTGGCAG CTGTGGTGCT GGTCCCTGTG 
TTTGCTGCCT TTGCTGTGTT GACTGTGGCC ATCCTGTTAG TGATGGAGGG GCTCTCAGCC 
TTCCTGCATG CCCTGCGGTT GCACTGGGTG GAGTTCCAGA ACAAGTTCTA TTCAGGCACC 
GGCTACAAGC TCAGCCCCTT CACCTTCACT GTGGACAGTG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001161256.1
CDS	465..2969
Translation	MGSMFRSEEVALVQLLLPTGSAYNCVSQLGELGLVEFRDLNESVSAFQRRFVVDVRRCEELEKTFTFLREEVQRAGLTLAPPEGTLPAPPPRDLLRIQEETDRLAQELRDVRGNQQALRAQLHQLRLHSAVLGQSHSPPVAADHTEGPFSETTPLLPGTRGPHSDLKVNFVAGAVEPYKAAALERLLWRACRGFLIASFRETEGQLEDPVTGEPATWMTFVISYWGEQIGQKIRKITDCFHCHVFPYLEQEEARFRTLQQLQQQSQELQEVLGETDRFLSQVLGRVQQLLPPWQVQIHKMKAVYLTLNQCSVNTTHKCLIAEVWCAARDLPTVQQALQSGSSEEGVSAVAHRIPCQDMPPTLIRTNRFTSSFQGIVDAYGVGRYREVNPAPYTIITFPFLFAVMFGDVGHGLLMFLFALAMVLTENRPAVKAAQNEIWQTFFGGRYLLLLMGLFSVYTGFIYNECFSRATTIFPSGWSVAAMANQSGWSDEYLSQHSMLTLNPNITGVFLGPYPFGIDPIWSLATNHLSFLNSFKMKMSVILGVTHMAFGVFLSIFNHVHFGQAHRLLLETLPELIFLLGLFGYLVFLIVYKWVNVSAASASSAPSILIHFINMFLFSQNPTNHLLFHGQEVVQYVLVVLALATVPILLLGTPLYLLRQHRHRRNTQRRPAGQQDEDTDKLLASPDASTLENSWSPDEEKAGSPGDEETEFVPSEIFMHQAIHTIEFCLGCISNTASYLRLWALSLAHAQLSEVLWAMVMRIGLGMGREIGVAAVVLVPVFAAFAVLTVAILLVMEGLSAFLHALRLHWVEFQNKFYSGTGYKLSPFTFTVDSD

Target ORF information:

RefSeq Version	NM_001167784.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus T cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 protein A3 (Tcirg1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001167784.1

ORF Insert Sequence:

ATGGGCTCTA TGTTCCGGAG TGAAGAGGTG GCCTTGGTCC AGCTCCTGTT GCCCACAGGG 
TCTGCTTACA ACTGTGTGAG CCAGCTTGGT GAGCTGGGCC TCGTGGAGTT CAGAGACCTC 
AACGAATCCG TGAGCGCCTT CCAGAGACGC TTCGTGGTAG ACGTCCGGCG CTGCGAGGAA 
CTGGAGAAGA CGTTTACCTT CTTGCGGGAA GAAGTGCAGC GGGCAGGTCT GACGCTGGCC 
CCACCTGAGG GGACACTGCC AGCACCACCT CCCCGAGACC TGCTGCGCAT CCAGGAGGAA 
ACGGATCGCT TGGCCCAGGA GCTTCGGGAT GTTCGTGGCA ATCAGCAGGC ACTGCGGGCC 
CAGCTGCACC AGCTGCGGCT TCACTCGGCT GTGCTGGGCC AGAGCCACAG CCCGCCGGTG 
GCAGCTGACC ATACTGAAGG ACCCTTCTCT GAGACAACAC CCCTGCTTCC AGGCACCCGG 
GGGCCACATT CAGACCTGAA GGTCAATTTT GTGGCAGGTG CTGTGGAGCC CTACAAGGCT 
GCGGCCCTGG AGCGCCTGCT GTGGAGAGCT TGCCGCGGCT TCCTCATTGC CAGCTTTCGG 
GAGACTGAGG GGCAGCTGGA GGACCCAGTG ACGGGTGAGC CTGCAACTTG GATGACCTTT 
GTCATCTCCT ACTGGGGTGA GCAGATCGGA CAGAAGATCC GAAAGATCAC AGACTGCTTC 
CACTGTCATG TCTTCCCATA CCTGGAGCAG GAGGAAGCCC GGTTCAGGAC CTTGCAGCAG 
CTGCAGCAGC AAAGCCAGGA GCTTCAGGAA GTCCTGGGGG AGACAGACCG GTTCCTGAGC 
CAGGTGTTGG GCCGGGTGCA GCAGCTGCTG CCCCCATGGC AGGTGCAGAT CCACAAGATG 
AAGGCAGTGT ACCTGACCCT CAACCAGTGC AGTGTGAACA CCACACACAA GTGCCTCATC 
GCGGAGGTCT GGTGTGCCGC GAGGGACCTG CCCACTGTGC AGCAGGCGCT GCAGAGCGGC 
TCAAGTGAGG AAGGAGTGAG TGCTGTCGCT CACCGCATCC CCTGCCAGGA CATGCCTCCA 
ACCCTCATCA GGACCAACCG CTTCACCTCC AGCTTCCAGG GCATTGTGGA TGCCTATGGT 
GTGGGCCGCT ACAGGGAAGT TAACCCTGCT CCCTACACCA TCATCACATT CCCCTTTCTC 
TTCGCTGTGA TGTTTGGCGA TGTGGGGCAC GGACTGCTCA TGTTTCTCTT TGCCCTGGCC 
ATGGTCCTCA CTGAAAACCG TCCAGCTGTG AAGGCTGCAC AAAACGAGAT CTGGCAGACC 
TTCTTTGGGG GTCGATACCT ACTCCTGCTC ATGGGTCTGT TCTCCGTCTA CACTGGCTTC 
ATCTACAATG AGTGCTTCAG CCGAGCCACC ACTATTTTCC CCTCAGGTTG GAGTGTGGCT 
GCCATGGCCA ACCAGTCAGG CTGGAGTGAC GAGTATCTGT CCCAGCACTC CATGCTCACC 
CTGAACCCTA ACATCACTGG TGTCTTCCTT GGACCATATC CCTTTGGCAT TGACCCGATC 
TGGAGCCTGG CCACGAACCA CCTGAGCTTT CTCAACTCCT TCAAGATGAA GATGTCTGTC 
ATCCTTGGGG TCACGCACAT GGCCTTTGGG GTGTTCCTCA GCATCTTCAA CCACGTGCAC 
TTTGGCCAGG CCCACCGGCT ACTGCTGGAG ACCTTGCCTG AGCTCATCTT CCTGCTGGGT 
CTCTTTGGCT ACCTTGTGTT CCTCATTGTC TACAAGTGGG TGAATGTCTC AGCTGCTAGC 
GCCTCCTCGG CCCCCAGTAT CCTCATTCAC TTCATCAATA TGTTCCTCTT CTCTCAAAAC 
CCCACCAATC ATCTGCTCTT CCATGGGCAG GAAGTGGTAC AGTACGTGCT GGTGGTCCTG 
GCTTTGGCTA CCGTTCCTAT CCTGTTGCTG GGCACACCCT TGTACCTGCT GCGCCAGCAC 
CGCCACAGAA GAAACACTCA GAGAAGGCCA GCAGGCCAGC AGGATGAGGA CACGGACAAG 
CTTCTGGCCT CCCCTGATGC ATCCACCTTG GAGAACAGCT GGAGCCCTGA TGAGGAGAAG 
GCTGGGAGCC CAGGGGATGA AGAAACTGAG TTTGTCCCTT CTGAGATCTT CATGCACCAA 
GCAATCCACA CCATTGAGTT CTGCCTGGGC TGCATCTCCA ACACAGCCTC CTACTTGCGT 
CTCTGGGCCC TGAGCCTGGC CCATGCCCAG CTGTCTGAGG TCCTGTGGGC CATGGTGATG 
CGCATAGGCC TGGGCATGGG CCGAGAGATC GGCGTGGCAG CTGTGGTGCT GGTCCCTGTG 
TTTGCTGCCT TTGCTGTGTT GACTGTGGCC ATCCTGTTAG TGATGGAGGG GCTCTCAGCC 
TTCCTGCATG CCCTGCGGTT GCACTGGGTG GAGTTCCAGA ACAAGTTCTA TTCAGGCACC 
GGCTACAAGC TCAGCCCCTT CACCTTCACT GTGGACAGTG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tcirg1 ( XM_011248632.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246934.1 Mus musculus T cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 protein A3 (Tcirg1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu69266
Clone ID Related Accession (Same CDS sequence)	XM_011248632.1
Accession Version	XM_011248632.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2052bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	V-type proton ATPase 116 kDa subunit a isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTCTA TGTTCCGGAG TGAAGAGGTG GCCTTGGTCC AGCTCCTGTT GCCCACAGGG 
TCTGCTTACA ACTGTGTGAG CCAGCTTGGT GAGCTGGGCC TCGTGGAGTT CAGAGACCTC 
AACGAATCCG TGAGCGCCTT CCAGAGACGC TTCGTGGTAG ACGTCCGGCG CTGCGAGGAA 
CTGGAGAAGA CGTTTACCTT CTTGCGGGAA GAAGTGCAGC GGGCAGGTCT GACGCTGGCC 
CCACCTGAGG GGACACTGCC AGCACCACCT CCCCGAGACC TGCTGCGCAT CCAGGAGGAA 
ACGGATCGCT TGGCCCAGGA GCTTCGGGAT GTTCGTGGCA ATCAGCAGGC ACTGCGGGCC 
CAGCTGCACC AGCTGCGGCT TCACTCGGCT GTGCTGGGCC AGAGCCACAG CCCGCCGGTG 
GCAGCTGACC ATACTGAAGG ACCCTTCTCT GAGACAACAC CCCTGCTTCC AGGCACCCGG 
GGGCCACATT CAGACCTGAA GGTCAATTTT GTGGCAGGTG CTGTGGAGCC CTACAAGGCT 
GCGGCCCTGG AGCGCCTGCT GTGGAGAGCT TGCCGCGGCT TCCTCATTGC CAGCTTTCGG 
GAGACTGAGG GGCAGCTGGA GGACCCAGTG ACGGGTGAGC CTGCAACTTG GATGACCTTT 
GTCATCTCCT ACTGGGGTGA GCAGATCGGA CAGAAGATCC GAAAGATCAC AGACTGCTTC 
CACTGTCATG TCTTCCCATA CCTGGAGCAG GAGGAAGCCC GGTTCAGGAC CTTGCAGCAG 
CTGCAGCAGC AAAGCCAGGA GCTTCAGGAA GTCCTGGGGG AGACAGACCG GTTCCTGAGC 
CAGGTGTTGG GCCGGGTGCA GCAGCTGCTG CCCCCATGGC AGGTGCAGAT CCACAAGATG 
AAGGCAGTGT ACCTGACCCT CAACCAGTGC AGTGTGAACA CCACACACAA GTGCCTCATC 
GCGGAGGTCT GGTGTGCCGC GAGGGACCTG CCCACTGTGC AGCAGGCGCT GCAGAGCGGC 
TCAAGTGAGG AAGGAGTGAG TGCTGTCGCT CACCGCATCC CCTGCCAGGA CATGCCTCCA 
ACCCTCATCA GGACCAACCG CTTCACCTCC AGCTTCCAGG GCATTGTGGA TGCCTATGGT 
GTGGGCCGCT ACAGGGAAGT TAACCCTGCT CCCTACACCA TCATCACATT CCCCTTTCTC 
TTCGCTGTGA TGTTTGGCGA TGTGGGGCAC GGACTGCTCA TGTTTCTCTT TGCCCTGGCC 
ATGGTCCTCA CTGAAAACCG TCCAGCTGTG AAGGCTGCAC AAAACGAGAT CTGGCAGACC 
TTCTTTGGGG GTCGATACCT ACTCCTGCTC ATGGGTCTGT TCTCCGTCTA CACTGGCTTC 
ATCTACAATG AGTGCTTCAG CCGAGCCACC ACTATTTTCC CCTCAGGTTG GAGTGTGGCT 
GCCATGGCCA ACCAGTCAGG CTGGAGTGAC GAGTATCTGT CCCAGCACTC CATGCTCACC 
CTGAACCCTA ACATCACTGG TGTCTTCCTT GGACCATATC CCTTTGGCAT TGACCCGATC 
TGGAGCCTGG CCACGAACCA CCTGAGCTTT CTCAACTCCT TCAAGATGAA GATGTCTGTC 
ATCCTTGGGG TCACGCACAT GGCCTTTGGG GTGTTCCTCA GCATCTTCAA CCACGTGCAC 
TTTGGCCAGG CCCACCGGCT ACTGCTGGAG ACCTTGCCTG AGCTCATCTT CCTGCTGGGT 
CTCTTTGGCT ACCTTGTGTT CCTCATTGTC TACAAGTGGG TGAATGTCTC AGCTGCTAGC 
GCCTCCTCGG CCCCCAGTAT CCTCATTCAC TTCATCAATA TGTTCCTCTT CTCTCAAAAC 
CCCACCAATC ATCTGCTCTT CCATGGGCAG GAAGTGGTAC AGTACGTGCT GGTGGTCCTG 
GCTTTGGCTA CCGTTCCTAT CCTGTTGCTG GGCACACCCT TGTACCTGCT GCGCCAGCAC 
CGCCACAGAA GAAACACTCA GAGAAGGCCA GCAGGCCAGC AGGTAGACGC AGCAGAAAGG 
ATTACAGGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246934.1
CDS	115..2166
Translation	MGSMFRSEEVALVQLLLPTGSAYNCVSQLGELGLVEFRDLNESVSAFQRRFVVDVRRCEELEKTFTFLREEVQRAGLTLAPPEGTLPAPPPRDLLRIQEETDRLAQELRDVRGNQQALRAQLHQLRLHSAVLGQSHSPPVAADHTEGPFSETTPLLPGTRGPHSDLKVNFVAGAVEPYKAAALERLLWRACRGFLIASFRETEGQLEDPVTGEPATWMTFVISYWGEQIGQKIRKITDCFHCHVFPYLEQEEARFRTLQQLQQQSQELQEVLGETDRFLSQVLGRVQQLLPPWQVQIHKMKAVYLTLNQCSVNTTHKCLIAEVWCAARDLPTVQQALQSGSSEEGVSAVAHRIPCQDMPPTLIRTNRFTSSFQGIVDAYGVGRYREVNPAPYTIITFPFLFAVMFGDVGHGLLMFLFALAMVLTENRPAVKAAQNEIWQTFFGGRYLLLLMGLFSVYTGFIYNECFSRATTIFPSGWSVAAMANQSGWSDEYLSQHSMLTLNPNITGVFLGPYPFGIDPIWSLATNHLSFLNSFKMKMSVILGVTHMAFGVFLSIFNHVHFGQAHRLLLETLPELIFLLGLFGYLVFLIVYKWVNVSAASASSAPSILIHFINMFLFSQNPTNHLLFHGQEVVQYVLVVLALATVPILLLGTPLYLLRQHRHRRNTQRRPAGQQVDAAERITG

Target ORF information:

RefSeq Version	XM_011248632.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus T cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 protein A3 (Tcirg1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248632.1

ORF Insert Sequence:

ATGGGCTCTA TGTTCCGGAG TGAAGAGGTG GCCTTGGTCC AGCTCCTGTT GCCCACAGGG 
TCTGCTTACA ACTGTGTGAG CCAGCTTGGT GAGCTGGGCC TCGTGGAGTT CAGAGACCTC 
AACGAATCCG TGAGCGCCTT CCAGAGACGC TTCGTGGTAG ACGTCCGGCG CTGCGAGGAA 
CTGGAGAAGA CGTTTACCTT CTTGCGGGAA GAAGTGCAGC GGGCAGGTCT GACGCTGGCC 
CCACCTGAGG GGACACTGCC AGCACCACCT CCCCGAGACC TGCTGCGCAT CCAGGAGGAA 
ACGGATCGCT TGGCCCAGGA GCTTCGGGAT GTTCGTGGCA ATCAGCAGGC ACTGCGGGCC 
CAGCTGCACC AGCTGCGGCT TCACTCGGCT GTGCTGGGCC AGAGCCACAG CCCGCCGGTG 
GCAGCTGACC ATACTGAAGG ACCCTTCTCT GAGACAACAC CCCTGCTTCC AGGCACCCGG 
GGGCCACATT CAGACCTGAA GGTCAATTTT GTGGCAGGTG CTGTGGAGCC CTACAAGGCT 
GCGGCCCTGG AGCGCCTGCT GTGGAGAGCT TGCCGCGGCT TCCTCATTGC CAGCTTTCGG 
GAGACTGAGG GGCAGCTGGA GGACCCAGTG ACGGGTGAGC CTGCAACTTG GATGACCTTT 
GTCATCTCCT ACTGGGGTGA GCAGATCGGA CAGAAGATCC GAAAGATCAC AGACTGCTTC 
CACTGTCATG TCTTCCCATA CCTGGAGCAG GAGGAAGCCC GGTTCAGGAC CTTGCAGCAG 
CTGCAGCAGC AAAGCCAGGA GCTTCAGGAA GTCCTGGGGG AGACAGACCG GTTCCTGAGC 
CAGGTGTTGG GCCGGGTGCA GCAGCTGCTG CCCCCATGGC AGGTGCAGAT CCACAAGATG 
AAGGCAGTGT ACCTGACCCT CAACCAGTGC AGTGTGAACA CCACACACAA GTGCCTCATC 
GCGGAGGTCT GGTGTGCCGC GAGGGACCTG CCCACTGTGC AGCAGGCGCT GCAGAGCGGC 
TCAAGTGAGG AAGGAGTGAG TGCTGTCGCT CACCGCATCC CCTGCCAGGA CATGCCTCCA 
ACCCTCATCA GGACCAACCG CTTCACCTCC AGCTTCCAGG GCATTGTGGA TGCCTATGGT 
GTGGGCCGCT ACAGGGAAGT TAACCCTGCT CCCTACACCA TCATCACATT CCCCTTTCTC 
TTCGCTGTGA TGTTTGGCGA TGTGGGGCAC GGACTGCTCA TGTTTCTCTT TGCCCTGGCC 
ATGGTCCTCA CTGAAAACCG TCCAGCTGTG AAGGCTGCAC AAAACGAGAT CTGGCAGACC 
TTCTTTGGGG GTCGATACCT ACTCCTGCTC ATGGGTCTGT TCTCCGTCTA CACTGGCTTC 
ATCTACAATG AGTGCTTCAG CCGAGCCACC ACTATTTTCC CCTCAGGTTG GAGTGTGGCT 
GCCATGGCCA ACCAGTCAGG CTGGAGTGAC GAGTATCTGT CCCAGCACTC CATGCTCACC 
CTGAACCCTA ACATCACTGG TGTCTTCCTT GGACCATATC CCTTTGGCAT TGACCCGATC 
TGGAGCCTGG CCACGAACCA CCTGAGCTTT CTCAACTCCT TCAAGATGAA GATGTCTGTC 
ATCCTTGGGG TCACGCACAT GGCCTTTGGG GTGTTCCTCA GCATCTTCAA CCACGTGCAC 
TTTGGCCAGG CCCACCGGCT ACTGCTGGAG ACCTTGCCTG AGCTCATCTT CCTGCTGGGT 
CTCTTTGGCT ACCTTGTGTT CCTCATTGTC TACAAGTGGG TGAATGTCTC AGCTGCTAGC 
GCCTCCTCGG CCCCCAGTAT CCTCATTCAC TTCATCAATA TGTTCCTCTT CTCTCAAAAC 
CCCACCAATC ATCTGCTCTT CCATGGGCAG GAAGTGGTAC AGTACGTGCT GGTGGTCCTG 
GCTTTGGCTA CCGTTCCTAT CCTGTTGCTG GGCACACCCT TGTACCTGCT GCGCCAGCAC 
CGCCACAGAA GAAACACTCA GAGAAGGCCA GCAGGCCAGC AGGTAGACGC AGCAGAAAGG 
ATTACAGGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Activity-independent targeting of mTOR to lysosomes in primary osteoclasts.
Scientific reports7(1)3005(2017 Jun)
Wang A,Carraro-Lacroix LR,Owen C,Gao B,Corey PN,Tyrrell P,Brumell JH,Voronov I

Oocyte-expressed yes-associated protein is a key activator of the early zygotic genome in mouse.
Cell research26(3)275-87(2016 Mar)
Yu C,Ji SY,Dang YJ,Sha QQ,Yuan YF,Zhou JJ,Yan LY,Qiao J,Tang F,Fan HY

Osteopetrorickets due to Snx10 deficiency in mice results from both failed osteoclast activity and loss of gastric acid-dependent calcium absorption.
PLoS genetics11(3)e1005057(2015 Mar)
Ye L,Morse LR,Zhang L,Sasaki H,Mills JC,Odgren PR,Sibbel G,Stanley JR,Wong G,Zamarioli A,Battaglino RA

The R740S mutation in the V-ATPase a3 subunit results in osteoclast apoptosis and defective early-stage autophagy.
Journal of cellular biochemistry114(12)2823-33(2013 Dec)
Ochotny N,Voronov I,Owen C,Aubin JE,Manolson MF

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Tcirg1 cDNA ORF clone, Mus musculus(house mouse)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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