Map1s cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Map1s
Entrez Gene ID	270058
Full Name	microtubule-associated protein 1S
Synonyms	6430517J16Rik,Bpy2ip1,Map8,Mtap1s,VCY2IP1
General protein information	Preferred Names microtubule-associated protein 1S Names microtubule-associated protein 1S BPY2 interacting protein 1 MAP-1S microtubule-associated protein 8
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8\|8 B3.3

mRNA and Protein(s)

mRNA	Protein	Name
XM_030243597.1	XP_030099457.1	microtubule-associated protein 1S isoform X1
XM_011242305.2	XP_011240607.1	microtubule-associated protein 1S isoform X1
NM_173013.3	NP_766601.2	microtubule-associated protein 1S

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	MAP1S	XP_003432881.2
Bos taurus (cattle)	MAP1S	NP_001095584.1
Rattus norvegicus (Norway rat)	Map1s	NP_001099540.1
Pan troglodytes (chimpanzee)	MAP1S	XP_512499.2
Homo sapiens (human)	MAP1S	NP_060644.4
	MAP1S	XP_001113855.1
Mus musculus (house mouse)	Map1s	NP_766601.2

Gene Ontology
Bibliography

Related articles in PubMed

Spermidine Prolongs Lifespan and Prevents Liver Fibrosis and Hepatocellular Carcinoma by Activating MAP1S-Mediated Autophagy.
Yue F, Li W, Zou J, Jiang X, Xu G, Huang H, Liu L
Cancer research77(11)2938-2951(2017 Jun)

Defects in MAP1S-mediated autophagy turnover of fibronectin cause renal fibrosis.
Xu G, Yue F, Huang H, He Y, Li X, Zhao H, Su Z, Jiang X, Li W, Zou J, Chen Q, Liu L
Aging8(5)977-85(2016 May)

Defects in MAP1S-mediated autophagy cause reduction in mouse lifespans especially when fibronectin is overexpressed.
Li W, Zou J, Yue F, Song K, Chen Q, McKeehan WL, Wang F, Xu G, Huang H, Yi J, Liu L
Aging cell15(2)370-9(2016 Apr)

MAP1S Protein Regulates the Phagocytosis of Bacteria and Toll-like Receptor (TLR) Signaling.
Shi M, Zhang Y, Liu L, Zhang T, Han F, Cleveland J, Wang F, McKeehan WL, Li Y, Zhang D
The Journal of biological chemistry291(3)1243-50(2016 Jan)

MAP1S enhances autophagy to suppress tumorigenesis.
Liu L, McKeehan WL, Wang F, Xie R
Autophagy8(2)278-80(2012 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Depletion of MAP1S in mice leads to an accumulation of fibrosis-related proteins and the development of renal fibrosis in aged mice.
Title: Defects in MAP1S-mediated autophagy turnover of fibronectin cause renal fibrosis.

MAP1S-mediated autophagy plays an important role in maintaining mouse lifespan especially in the presence of extra amount of fibronectin.
Title: Defects in MAP1S-mediated autophagy cause reduction in mouse lifespans especially when fibronectin is overexpressed.

Data (including data from studies in knockout mice) suggest that Map1s interacts directly with MyD88 (myeloid differentiation primary response gene 88), a key adaptor of Toll-like receptors, as phagocytosis of bacteria is initiated in macrophages.
Title: MAP1S Protein Regulates the Phagocytosis of Bacteria and Toll-like Receptor (TLR) Signaling.

Data establish a link between MAP1S-enhanced autophagy and suppression of genomic instability and tumorigenesis.
Title: Autophagy enhanced by microtubule- and mitochondrion-associated MAP1S suppresses genome instability and hepatocarcinogenesis.

clarify roles of MAP1S in bridging microtubules and mitochondria with autophagic and mitophagic initiation, maturation, trafficking, and lysosomal clearance.
Title: Microtubule-associated protein 1S (MAP1S) bridges autophagic components with microtubules and mitochondria to affect autophagosomal biogenesis and degradation.

The following Map1s gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Map1s cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu04408	NM_173013.3 Latest version!	Mus musculus microtubule-associated protein 1S (Map1s), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$573.30 $819.00
OMu74443	XM_011242305.2 Latest version!	Mus musculus microtubule-associated protein 1S (Map1s), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu74443	XM_030243597.1 Latest version!	Mus musculus microtubule-associated protein 1S (Map1s), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Map1s ( NM_173013.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_766601.2 Mus musculus microtubule-associated protein 1S (Map1s), mRNA.

Price & Availability↑

CloneID	OMu04408
Clone ID Related Accession (Same CDS sequence)	NM_173013.3
Accession Version	NM_173013.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2922bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	microtubule-associated protein 1S
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB857804.1, AK032300.1, AK162124.1 and AA048974.1. On Dec 11, 2007 this sequence version replaced NM_173013.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK082854.1, SRR1660815.91214.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGCAG TGATGGCGGC GCCGGAGGCT GTGGAGGCGC CCAGCTCCCT ACTGTTGCTG 
GTGGTGGGTG GCGAGTGTGG CTGCCCCGGG CTGCTGGCCT ACGTCATGGA GGAGTTGGAA 
CGAGGTGTTC GCTCCTGGGA GGATGTGGAC CCCGCTGTCT GCAGCCTGGA TGAGCAGCTC 
AAGGCCTTCG TGTCCCGGCA TTCAGCCACC TTCTCCAGCA TTGTGAAAGG GCAGCGGAGC 
CTGCACCACC GTGGAGAGAC CTTGGAGACG CTGGTCCTGC TGAACCCCTC AGACAAGTCC 
CTGTGTGATG AGCTCCGGAA CCTCCTGATG GACCCCGCCC CTCACAAGCT GCTGGTCCTG 
GCCGGGCCCT GCCTGGAGGA GACTGGGGAA CTGCTGTTGC AGACTGGGGG GTTCTCGGCC 
CACCACTTCC TGCAGGTGCT GGGGGACAAA GAGGTCCAGG ATGCACTGGC CTCAGCACCA 
GCTGCCCCAG CCCTCACTGT GTCCTGTCCA ACCTTTGGGG ACTGGGCACT GTTGGGCCCT 
GTGCCCGGGC TGCAGCTGCG ACTAAATCCG CCCGCGCAGC TGCCGGCCTC AGAGGGCCTT 
CGTGCCTTCC TGGAGTATGT GGCTGAGTCG CTGGAGCCGC CATCGCCCTT CGAGTTGTTG 
GAGCCACCGG CTGCGGGCGG CTTTCTGCGC TTAGCAAGGC CCTGTTGCTA TGTGTTCCCG 
GGTGGCTTGG GAGACGCCGC CTTCTTCGCT GTCAACGGCT TTACAGTGCT GGTGAATGGT 
GGCTCCAACC CCAAGTCGAG CTTCTGGAAG CTGGTGCGCC ACCTGGATCG CGTGGACGCT 
GTCCTTGTGA CCCACGCGGG TGCTGACAGC TTGCCGGGCC TCAACAGCCT GCTGCGCCGC 
AAGCTGGCAG AGCGCGAAGC AGCCGCAGGA CCACAGGGAC AGCATGAGGA GCGGCTACGT 
CGCCTGCTGT CGCCTGCGCT GGGTGTGGTG TTCCTGAATG CTCGTGAGGC TGCTTCTCGG 
TTGCGTGGGG GCGAAGACGA GGCTGTGTGT GCTCGGAGCC TGCTGCGGAG CCTGGGCATT 
GCGCCTCTGC CACTGCAGCG TGGCCCACAG CCCTCTTGCC CCACTGTCCT TTTCGAGAAG 
CTGGGCGTAG GCCGCCTAGA GCTTTTTGTG CTGCACCCTC CTCCTGGGGA CCCTGCAGCG 
CCTGCCTGTG CTCTGCTCGT GTGGCAGCCA GCGGCGCCTG GCGACAAGGT TGTGCGTGTA 
CTATTTCCTG GCCGCACGCC GCCCGCGCGC CTGCTGGATG GGCTACAGCG CCTGCAGCAC 
CTGCCGTGCC TGCGCAGGCC AGTAGTTACC ACGCATGACC TGGAAGCGCC CTCCAGGGCC 
AACAGCCAAG ACAGTCTGGC CTCGCGGGAC AGTGCACGCA AGGAGCCAGT CCGTGGCACT 
GTTGGCAGCA TTGCCAACAG GAGTACAGTG CGAAGGGAGC CTGCCCTGGC CACTCGTGAC 
CAGAAGAAGG ACACAAGATC TGGGCCCACT CAGCCTACTG CCCGAGACAC ACGCCGCTCC 
GGGCCTGGTG TGGTCAACAC AAAGCCTCGT GTATCACAGA ATGGGCCTCG TGCCCCAGTT 
CTGGCAGCGC CACTCACAGC TCCTGTGGCT GAGTGCCCTG GAGAAGCGGA GAACATCTTG 
GAGTCTGAGC GGCCACCTGC CCCATCCCCA ACTCTGTCCC CAGCTCAGTC CCCACCACCT 
ACAGCACCTG GTAATAGCCC TGAGCGCCTG TCACTCAGCC CTCTGCGCCC AGAGCCTGCC 
CCTGATGCGT CGCCCTCAGC CACGACGCCC ACGCTGACCA CGCCCTCTTT GCCTGCGGAG 
CTGGGCTCAC CCCACTCAAC TGAGGTGGAC GAGTCGCTCT CTGTGTCTTT CGAGCAAGTC 
CTGCCAGCAG GTGACCCAGG GCTTAGCCTG CCCCTGCGCC TCGCGCGCCG CTCCACGTCT 
CCTCATGATG TGGACCTGTG CCTCGTGTCA CCCTGTGAGT TCTCTCATCG CAAGCCACCC 
CCTCCTGCCT CCCCAGGCAG CTCGGACAGC AGTGCTCGGT CACAGGAGCG GCCACCTGAG 
ACTCCACCCA CGTCTGTGAG CGAGTCACTG CCCACACTAT CTGACTCTGA TCCCGTGCCC 
GTGGCGGATT CTGATGATGA CGCAGGCAGC GAGAGCGCGG CCAGGGACCC GCCGCCTACA 
CCTCGTGTGC CACCACCACT GCCAGATGTA CCAGGAATCT GCATGGTGGA CCCGGAGGCG 
CTACCGCCTC GTGCCCGGCA GCCCCTCAAC ACCACCAATC CCAGCCGTAG CCGCAAGGCC 
CCTGCAAGGC CTAGCTCTGC CTCTGCCACC CCCAGAGCTG CAACTGTAGC TGCCAAGACA 
AAGGGCCCCG CGGGGGACCG GAACCGGCCA CTCAGCGCCC GCAGTGAGCC TGCAGACAGG 
CCAGGCCGTG TGCCCCTTAC CAGGAAGCCC TCAGTCCCTA AGACCGTTCC CAAGATGGCC 
TCTGCCACAA GGCTCTCCTC CGGACCCAGT GGCCGCCCTG CACCGCTTGC TGCTGGCTCC 
CCTGTATACC TGGACCTGGC CTACCTGCCT GGTGGTGGTG CCGGTCACCT GGACCAGAAC 
TTCTTCCTGC GTGTCCGTGC ACTCTGCTAT GTCATCAGTG GGCAAGGCCA GCGCCAGGAA 
GAGGGCCTGC GAGCTGTGCT GGATGCACTG CTGGCTGGCA AGCGGCAGTG GGACCTTGAC 
TTGCAGGTCA CACTGATCCC CACCTTCGAC TCTGCGGTGA TGCACAGGTG GTATGAGGAG 
ACGCATGCGC AGCACCAGGC ACTGGGCATC AGGGTGCTGG GCAGTGGCAG CCTGGTGTCC 
ATGCAGGACG AGGCCTTCCC CGCCTGCAAG GTGGAGTTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_766601.2
CDS	31..2952
Translation	MAAVMAAPEAVEAPSSLLLLVVGGECGCPGLLAYVMEELERGVRSWEDVDPAVCSLDEQLKAFVSRHSATFSSIVKGQRSLHHRGETLETLVLLNPSDKSLCDELRNLLMDPAPHKLLVLAGPCLEETGELLLQTGGFSAHHFLQVLGDKEVQDALASAPAAPALTVSCPTFGDWALLGPVPGLQLRLNPPAQLPASEGLRAFLEYVAESLEPPSPFELLEPPAAGGFLRLARPCCYVFPGGLGDAAFFAVNGFTVLVNGGSNPKSSFWKLVRHLDRVDAVLVTHAGADSLPGLNSLLRRKLAEREAAAGPQGQHEERLRRLLSPALGVVFLNAREAASRLRGGEDEAVCARSLLRSLGIAPLPLQRGPQPSCPTVLFEKLGVGRLELFVLHPPPGDPAAPACALLVWQPAAPGDKVVRVLFPGRTPPARLLDGLQRLQHLPCLRRPVVTTHDLEAPSRANSQDSLASRDSARKEPVRGTVGSIANRSTVRREPALATRDQKKDTRSGPTQPTARDTRRSGPGVVNTKPRVSQNGPRAPVLAAPLTAPVAECPGEAENILESERPPAPSPTLSPAQSPPPTAPGNSPERLSLSPLRPEPAPDASPSATTPTLTTPSLPAELGSPHSTEVDESLSVSFEQVLPAGDPGLSLPLRLARRSTSPHDVDLCLVSPCEFSHRKPPPPASPGSSDSSARSQERPPETPPTSVSESLPTLSDSDPVPVADSDDDAGSESAARDPPPTPRVPPPLPDVPGICMVDPEALPPRARQPLNTTNPSRSRKAPARPSSASATPRAATVAAKTKGPAGDRNRPLSARSEPADRPGRVPLTRKPSVPKTVPKMASATRLSSGPSGRPAPLAAGSPVYLDLAYLPGGGAGHLDQNFFLRVRALCYVISGQGQRQEEGLRAVLDALLAGKRQWDLDLQVTLIPTFDSAVMHRWYEETHAQHQALGIRVLGSGSLVSMQDEAFPACKVEF

Target ORF information:

RefSeq Version	NM_173013.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus microtubule-associated protein 1S (Map1s), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173013.3

ORF Insert Sequence:

ATGGCGGCAG TGATGGCGGC GCCGGAGGCT GTGGAGGCGC CCAGCTCCCT ACTGTTGCTG 
GTGGTGGGTG GCGAGTGTGG CTGCCCCGGG CTGCTGGCCT ACGTCATGGA GGAGTTGGAA 
CGAGGTGTTC GCTCCTGGGA GGATGTGGAC CCCGCTGTCT GCAGCCTGGA TGAGCAGCTC 
AAGGCCTTCG TGTCCCGGCA TTCAGCCACC TTCTCCAGCA TTGTGAAAGG GCAGCGGAGC 
CTGCACCACC GTGGAGAGAC CTTGGAGACG CTGGTCCTGC TGAACCCCTC AGACAAGTCC 
CTGTGTGATG AGCTCCGGAA CCTCCTGATG GACCCCGCCC CTCACAAGCT GCTGGTCCTG 
GCCGGGCCCT GCCTGGAGGA GACTGGGGAA CTGCTGTTGC AGACTGGGGG GTTCTCGGCC 
CACCACTTCC TGCAGGTGCT GGGGGACAAA GAGGTCCAGG ATGCACTGGC CTCAGCACCA 
GCTGCCCCAG CCCTCACTGT GTCCTGTCCA ACCTTTGGGG ACTGGGCACT GTTGGGCCCT 
GTGCCCGGGC TGCAGCTGCG ACTAAATCCG CCCGCGCAGC TGCCGGCCTC AGAGGGCCTT 
CGTGCCTTCC TGGAGTATGT GGCTGAGTCG CTGGAGCCGC CATCGCCCTT CGAGTTGTTG 
GAGCCACCGG CTGCGGGCGG CTTTCTGCGC TTAGCAAGGC CCTGTTGCTA TGTGTTCCCG 
GGTGGCTTGG GAGACGCCGC CTTCTTCGCT GTCAACGGCT TTACAGTGCT GGTGAATGGT 
GGCTCCAACC CCAAGTCGAG CTTCTGGAAG CTGGTGCGCC ACCTGGATCG CGTGGACGCT 
GTCCTTGTGA CCCACGCGGG TGCTGACAGC TTGCCGGGCC TCAACAGCCT GCTGCGCCGC 
AAGCTGGCAG AGCGCGAAGC AGCCGCAGGA CCACAGGGAC AGCATGAGGA GCGGCTACGT 
CGCCTGCTGT CGCCTGCGCT GGGTGTGGTG TTCCTGAATG CTCGTGAGGC TGCTTCTCGG 
TTGCGTGGGG GCGAAGACGA GGCTGTGTGT GCTCGGAGCC TGCTGCGGAG CCTGGGCATT 
GCGCCTCTGC CACTGCAGCG TGGCCCACAG CCCTCTTGCC CCACTGTCCT TTTCGAGAAG 
CTGGGCGTAG GCCGCCTAGA GCTTTTTGTG CTGCACCCTC CTCCTGGGGA CCCTGCAGCG 
CCTGCCTGTG CTCTGCTCGT GTGGCAGCCA GCGGCGCCTG GCGACAAGGT TGTGCGTGTA 
CTATTTCCTG GCCGCACGCC GCCCGCGCGC CTGCTGGATG GGCTACAGCG CCTGCAGCAC 
CTGCCGTGCC TGCGCAGGCC AGTAGTTACC ACGCATGACC TGGAAGCGCC CTCCAGGGCC 
AACAGCCAAG ACAGTCTGGC CTCGCGGGAC AGTGCACGCA AGGAGCCAGT CCGTGGCACT 
GTTGGCAGCA TTGCCAACAG GAGTACAGTG CGAAGGGAGC CTGCCCTGGC CACTCGTGAC 
CAGAAGAAGG ACACAAGATC TGGGCCCACT CAGCCTACTG CCCGAGACAC ACGCCGCTCC 
GGGCCTGGTG TGGTCAACAC AAAGCCTCGT GTATCACAGA ATGGGCCTCG TGCCCCAGTT 
CTGGCAGCGC CACTCACAGC TCCTGTGGCT GAGTGCCCTG GAGAAGCGGA GAACATCTTG 
GAGTCTGAGC GGCCACCTGC CCCATCCCCA ACTCTGTCCC CAGCTCAGTC CCCACCACCT 
ACAGCACCTG GTAATAGCCC TGAGCGCCTG TCACTCAGCC CTCTGCGCCC AGAGCCTGCC 
CCTGATGCGT CGCCCTCAGC CACGACGCCC ACGCTGACCA CGCCCTCTTT GCCTGCGGAG 
CTGGGCTCAC CCCACTCAAC TGAGGTGGAC GAGTCGCTCT CTGTGTCTTT CGAGCAAGTC 
CTGCCAGCAG GTGACCCAGG GCTTAGCCTG CCCCTGCGCC TCGCGCGCCG CTCCACGTCT 
CCTCATGATG TGGACCTGTG CCTCGTGTCA CCCTGTGAGT TCTCTCATCG CAAGCCACCC 
CCTCCTGCCT CCCCAGGCAG CTCGGACAGC AGTGCTCGGT CACAGGAGCG GCCACCTGAG 
ACTCCACCCA CGTCTGTGAG CGAGTCACTG CCCACACTAT CTGACTCTGA TCCCGTGCCC 
GTGGCGGATT CTGATGATGA CGCAGGCAGC GAGAGCGCGG CCAGGGACCC GCCGCCTACA 
CCTCGTGTGC CACCACCACT GCCAGATGTA CCAGGAATCT GCATGGTGGA CCCGGAGGCG 
CTACCGCCTC GTGCCCGGCA GCCCCTCAAC ACCACCAATC CCAGCCGTAG CCGCAAGGCC 
CCTGCAAGGC CTAGCTCTGC CTCTGCCACC CCCAGAGCTG CAACTGTAGC TGCCAAGACA 
AAGGGCCCCG CGGGGGACCG GAACCGGCCA CTCAGCGCCC GCAGTGAGCC TGCAGACAGG 
CCAGGCCGTG TGCCCCTTAC CAGGAAGCCC TCAGTCCCTA AGACCGTTCC CAAGATGGCC 
TCTGCCACAA GGCTCTCCTC CGGACCCAGT GGCCGCCCTG CACCGCTTGC TGCTGGCTCC 
CCTGTATACC TGGACCTGGC CTACCTGCCT GGTGGTGGTG CCGGTCACCT GGACCAGAAC 
TTCTTCCTGC GTGTCCGTGC ACTCTGCTAT GTCATCAGTG GGCAAGGCCA GCGCCAGGAA 
GAGGGCCTGC GAGCTGTGCT GGATGCACTG CTGGCTGGCA AGCGGCAGTG GGACCTTGAC 
TTGCAGGTCA CACTGATCCC CACCTTCGAC TCTGCGGTGA TGCACAGGTG GTATGAGGAG 
ACGCATGCGC AGCACCAGGC ACTGGGCATC AGGGTGCTGG GCAGTGGCAG CCTGGTGTCC 
ATGCAGGACG AGGCCTTCCC CGCCTGCAAG GTGGAGTTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Map1s ( XM_011242305.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011240607.1 Mus musculus microtubule-associated protein 1S (Map1s), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu74443
Clone ID Related Accession (Same CDS sequence)	XM_030243597.1 , XM_011242305.2
Accession Version	XM_011242305.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2676bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	microtubule-associated protein 1S isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011242305.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACAAA TTGATCTGGG AATGTTGTCA CAGGCCTGTT GTCCCAGCAG GAGGAGGCAG 
AAGCAGCTCC GGAACCTCCT GATGGACCCC GCCCCTCACA AGCTGCTGGT CCTGGCCGGG 
CCCTGCCTGG AGGAGACTGG GGAACTGCTG TTGCAGACTG GGGGGTTCTC GGCCCACCAC 
TTCCTGCAGG TGCTGGGGGA CAAAGAGGTC CAGGATGCAC TGGCCTCAGC ACCAGCTGCC 
CCAGCCCTCA CTGTGTCCTG TCCAACCTTT GGGGACTGGG CACTGTTGGG CCCTGTGCCC 
GGGCTGCAGC TGCGACTAAA TCCGCCCGCG CAGCTGCCGG CCTCAGAGGG CCTTCGTGCC 
TTCCTGGAGT ATGTGGCTGA GTCGCTGGAG CCGCCATCGC CCTTCGAGTT GTTGGAGCCA 
CCGGCTGCGG GCGGCTTTCT GCGCTTAGCA AGGCCCTGTT GCTATGTGTT CCCGGGTGGC 
TTGGGAGACG CCGCCTTCTT CGCTGTCAAC GGCTTTACAG TGCTGGTGAA TGGTGGCTCC 
AACCCCAAGT CGAGCTTCTG GAAGCTGGTG CGCCACCTGG ATCGCGTGGA CGCTGTCCTT 
GTGACCCACG CGGGTGCTGA CAGCTTGCCG GGCCTCAACA GCCTGCTGCG CCGCAAGCTG 
GCAGAGCGCG AAGCAGCCGC AGGACCACAG GGACAGCATG AGGAGCGGCT ACGTCGCCTG 
CTGTCGCCTG CGCTGGGTGT GGTGTTCCTG AATGCTCGTG AGGCTGCTTC TCGGTTGCGT 
GGGGGCGAAG ACGAGGCTGT GTGTGCTCGG AGCCTGCTGC GGAGCCTGGG CATTGCGCCT 
CTGCCACTGC AGCGTGGCCC ACAGCCCTCT TGCCCCACTG TCCTTTTCGA GAAGCTGGGC 
GTAGGCCGCC TAGAGCTTTT TGTGCTGCAC CCTCCTCCTG GGGACCCTGC AGCGCCTGCC 
TGTGCTCTGC TCGTGTGGCA GCCAGCGGCG CCTGGCGACA AGGTTGTGCG TGTACTATTT 
CCTGGCCGCA CGCCGCCCGC GCGCCTGCTG GATGGGCTAC AGCGCCTGCA GCACCTGCCG 
TGCCTGCGCA GGCCAGTAGT TACCACGCAT GACCTGGAAG CGCCCTCCAG GGCCAACAGC 
CAAGACAGTC TGGCCTCGCG GGACAGTGCA CGCAAGGAGC CAGTCCGTGG CACTGTTGGC 
AGCATTGCCA ACAGGAGTAC AGTGCGAAGG GAGCCTGCCC TGGCCACTCG TGACCAGAAG 
AAGGACACAA GATCTGGGCC CACTCAGCCT ACTGCCCGAG ACACACGCCG CTCCGGGCCT 
GGTGTGGTCA ACACAAAGCC TCGTGTATCA CAGAATGGGC CTCGTGCCCC AGTTCTGGCA 
GCGCCACTCA CAGCTCCTGT GGCTGAGTGC CCTGGAGAAG CGGAGAACAT CTTGGAGTCT 
GAGCGGCCAC CTGCCCCATC CCCAACTCTG TCCCCAGCTC AGTCCCCACC ACCTACAGCA 
CCTGGTAATA GCCCTGAGCG CCTGTCACTC AGCCCTCTGC GCCCAGAGCC TGCCCCTGAT 
GCGTCGCCCT CAGCCACGAC GCCCACGCTG ACCACGCCCT CTTTGCCTGC GGAGCTGGGC 
TCACCCCACT CAACTGAGGT GGACGAGTCG CTCTCTGTGT CTTTCGAGCA AGTCCTGCCA 
GCAGGTGACC CAGGGCTTAG CCTGCCCCTG CGCCTCGCGC GCCGCTCCAC GTCTCCTCAT 
GATGTGGACC TGTGCCTCGT GTCACCCTGT GAGTTCTCTC ATCGCAAGCC ACCCCCTCCT 
GCCTCCCCAG GCAGCTCGGA CAGCAGTGCT CGGTCACAGG AGCGGCCACC TGAGACTCCA 
CCCACGTCTG TGAGCGAGTC ACTGCCCACA CTATCTGACT CTGATCCCGT GCCCGTGGCG 
GATTCTGATG ATGACGCAGG CAGCGAGAGC GCGGCCAGGG ACCCGCCGCC TACACCTCGT 
GTGCCACCAC CACTGCCAGA TGTACCAGGA ATCTGCATGG TGGACCCGGA GGCGCTACCG 
CCTCGTGCCC GGCAGCCCCT CAACACCACC AATCCCAGCC GTAGCCGCAA GGCCCCTGCA 
AGGCCTAGCT CTGCCTCTGC CACCCCCAGA GCTGCAACTG TAGCTGCCAA GACAAAGGGC 
CCCGCGGGGG ACCGGAACCG GCCACTCAGC GCCCGCAGTG AGCCTGCAGA CAGGCCAGGC 
CGTGTGCCCC TTACCAGGAA GCCCTCAGTC CCTAAGACCG TTCCCAAGAT GGCCTCTGCC 
ACAAGGCTCT CCTCCGGACC CAGTGGCCGC CCTGCACCGC TTGCTGCTGG CTCCCCTGTA 
TACCTGGACC TGGCCTACCT GCCTGGTGGT GGTGCCGGTC ACCTGGACCA GAACTTCTTC 
CTGCGTGTCC GTGCACTCTG CTATGTCATC AGTGGGCAAG GCCAGCGCCA GGAAGAGGGC 
CTGCGAGCTG TGCTGGATGC ACTGCTGGCT GGCAAGCGGC AGTGGGACCT TGACTTGCAG 
GTCACACTGA TCCCCACCTT CGACTCTGCG GTGATGCACA GGTGGTATGA GGAGACGCAT 
GCGCAGCACC AGGCACTGGG CATCAGGGTG CTGGGCAGTG GCAGCCTGGT GTCCATGCAG 
GACGAGGCCT TCCCCGCCTG CAAGGTGGAG TTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011240607.1
CDS	756..3431
Translation	MSQIDLGMLSQACCPSRRRQKQLRNLLMDPAPHKLLVLAGPCLEETGELLLQTGGFSAHHFLQVLGDKEVQDALASAPAAPALTVSCPTFGDWALLGPVPGLQLRLNPPAQLPASEGLRAFLEYVAESLEPPSPFELLEPPAAGGFLRLARPCCYVFPGGLGDAAFFAVNGFTVLVNGGSNPKSSFWKLVRHLDRVDAVLVTHAGADSLPGLNSLLRRKLAEREAAAGPQGQHEERLRRLLSPALGVVFLNAREAASRLRGGEDEAVCARSLLRSLGIAPLPLQRGPQPSCPTVLFEKLGVGRLELFVLHPPPGDPAAPACALLVWQPAAPGDKVVRVLFPGRTPPARLLDGLQRLQHLPCLRRPVVTTHDLEAPSRANSQDSLASRDSARKEPVRGTVGSIANRSTVRREPALATRDQKKDTRSGPTQPTARDTRRSGPGVVNTKPRVSQNGPRAPVLAAPLTAPVAECPGEAENILESERPPAPSPTLSPAQSPPPTAPGNSPERLSLSPLRPEPAPDASPSATTPTLTTPSLPAELGSPHSTEVDESLSVSFEQVLPAGDPGLSLPLRLARRSTSPHDVDLCLVSPCEFSHRKPPPPASPGSSDSSARSQERPPETPPTSVSESLPTLSDSDPVPVADSDDDAGSESAARDPPPTPRVPPPLPDVPGICMVDPEALPPRARQPLNTTNPSRSRKAPARPSSASATPRAATVAAKTKGPAGDRNRPLSARSEPADRPGRVPLTRKPSVPKTVPKMASATRLSSGPSGRPAPLAAGSPVYLDLAYLPGGGAGHLDQNFFLRVRALCYVISGQGQRQEEGLRAVLDALLAGKRQWDLDLQVTLIPTFDSAVMHRWYEETHAQHQALGIRVLGSGSLVSMQDEAFPACKVEF

Target ORF information:

RefSeq Version	XM_011242305.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus microtubule-associated protein 1S (Map1s), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011242305.2

ORF Insert Sequence:

ATGTCACAAA TTGATCTGGG AATGTTGTCA CAGGCCTGTT GTCCCAGCAG GAGGAGGCAG 
AAGCAGCTCC GGAACCTCCT GATGGACCCC GCCCCTCACA AGCTGCTGGT CCTGGCCGGG 
CCCTGCCTGG AGGAGACTGG GGAACTGCTG TTGCAGACTG GGGGGTTCTC GGCCCACCAC 
TTCCTGCAGG TGCTGGGGGA CAAAGAGGTC CAGGATGCAC TGGCCTCAGC ACCAGCTGCC 
CCAGCCCTCA CTGTGTCCTG TCCAACCTTT GGGGACTGGG CACTGTTGGG CCCTGTGCCC 
GGGCTGCAGC TGCGACTAAA TCCGCCCGCG CAGCTGCCGG CCTCAGAGGG CCTTCGTGCC 
TTCCTGGAGT ATGTGGCTGA GTCGCTGGAG CCGCCATCGC CCTTCGAGTT GTTGGAGCCA 
CCGGCTGCGG GCGGCTTTCT GCGCTTAGCA AGGCCCTGTT GCTATGTGTT CCCGGGTGGC 
TTGGGAGACG CCGCCTTCTT CGCTGTCAAC GGCTTTACAG TGCTGGTGAA TGGTGGCTCC 
AACCCCAAGT CGAGCTTCTG GAAGCTGGTG CGCCACCTGG ATCGCGTGGA CGCTGTCCTT 
GTGACCCACG CGGGTGCTGA CAGCTTGCCG GGCCTCAACA GCCTGCTGCG CCGCAAGCTG 
GCAGAGCGCG AAGCAGCCGC AGGACCACAG GGACAGCATG AGGAGCGGCT ACGTCGCCTG 
CTGTCGCCTG CGCTGGGTGT GGTGTTCCTG AATGCTCGTG AGGCTGCTTC TCGGTTGCGT 
GGGGGCGAAG ACGAGGCTGT GTGTGCTCGG AGCCTGCTGC GGAGCCTGGG CATTGCGCCT 
CTGCCACTGC AGCGTGGCCC ACAGCCCTCT TGCCCCACTG TCCTTTTCGA GAAGCTGGGC 
GTAGGCCGCC TAGAGCTTTT TGTGCTGCAC CCTCCTCCTG GGGACCCTGC AGCGCCTGCC 
TGTGCTCTGC TCGTGTGGCA GCCAGCGGCG CCTGGCGACA AGGTTGTGCG TGTACTATTT 
CCTGGCCGCA CGCCGCCCGC GCGCCTGCTG GATGGGCTAC AGCGCCTGCA GCACCTGCCG 
TGCCTGCGCA GGCCAGTAGT TACCACGCAT GACCTGGAAG CGCCCTCCAG GGCCAACAGC 
CAAGACAGTC TGGCCTCGCG GGACAGTGCA CGCAAGGAGC CAGTCCGTGG CACTGTTGGC 
AGCATTGCCA ACAGGAGTAC AGTGCGAAGG GAGCCTGCCC TGGCCACTCG TGACCAGAAG 
AAGGACACAA GATCTGGGCC CACTCAGCCT ACTGCCCGAG ACACACGCCG CTCCGGGCCT 
GGTGTGGTCA ACACAAAGCC TCGTGTATCA CAGAATGGGC CTCGTGCCCC AGTTCTGGCA 
GCGCCACTCA CAGCTCCTGT GGCTGAGTGC CCTGGAGAAG CGGAGAACAT CTTGGAGTCT 
GAGCGGCCAC CTGCCCCATC CCCAACTCTG TCCCCAGCTC AGTCCCCACC ACCTACAGCA 
CCTGGTAATA GCCCTGAGCG CCTGTCACTC AGCCCTCTGC GCCCAGAGCC TGCCCCTGAT 
GCGTCGCCCT CAGCCACGAC GCCCACGCTG ACCACGCCCT CTTTGCCTGC GGAGCTGGGC 
TCACCCCACT CAACTGAGGT GGACGAGTCG CTCTCTGTGT CTTTCGAGCA AGTCCTGCCA 
GCAGGTGACC CAGGGCTTAG CCTGCCCCTG CGCCTCGCGC GCCGCTCCAC GTCTCCTCAT 
GATGTGGACC TGTGCCTCGT GTCACCCTGT GAGTTCTCTC ATCGCAAGCC ACCCCCTCCT 
GCCTCCCCAG GCAGCTCGGA CAGCAGTGCT CGGTCACAGG AGCGGCCACC TGAGACTCCA 
CCCACGTCTG TGAGCGAGTC ACTGCCCACA CTATCTGACT CTGATCCCGT GCCCGTGGCG 
GATTCTGATG ATGACGCAGG CAGCGAGAGC GCGGCCAGGG ACCCGCCGCC TACACCTCGT 
GTGCCACCAC CACTGCCAGA TGTACCAGGA ATCTGCATGG TGGACCCGGA GGCGCTACCG 
CCTCGTGCCC GGCAGCCCCT CAACACCACC AATCCCAGCC GTAGCCGCAA GGCCCCTGCA 
AGGCCTAGCT CTGCCTCTGC CACCCCCAGA GCTGCAACTG TAGCTGCCAA GACAAAGGGC 
CCCGCGGGGG ACCGGAACCG GCCACTCAGC GCCCGCAGTG AGCCTGCAGA CAGGCCAGGC 
CGTGTGCCCC TTACCAGGAA GCCCTCAGTC CCTAAGACCG TTCCCAAGAT GGCCTCTGCC 
ACAAGGCTCT CCTCCGGACC CAGTGGCCGC CCTGCACCGC TTGCTGCTGG CTCCCCTGTA 
TACCTGGACC TGGCCTACCT GCCTGGTGGT GGTGCCGGTC ACCTGGACCA GAACTTCTTC 
CTGCGTGTCC GTGCACTCTG CTATGTCATC AGTGGGCAAG GCCAGCGCCA GGAAGAGGGC 
CTGCGAGCTG TGCTGGATGC ACTGCTGGCT GGCAAGCGGC AGTGGGACCT TGACTTGCAG 
GTCACACTGA TCCCCACCTT CGACTCTGCG GTGATGCACA GGTGGTATGA GGAGACGCAT 
GCGCAGCACC AGGCACTGGG CATCAGGGTG CTGGGCAGTG GCAGCCTGGT GTCCATGCAG 
GACGAGGCCT TCCCCGCCTG CAAGGTGGAG TTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Map1s ( XM_030243597.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030099457.1 Mus musculus microtubule-associated protein 1S (Map1s), transcript variant X1, mRNA.

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CloneID	OMu74443
Clone ID Related Accession (Same CDS sequence)	XM_030243597.1 , XM_011242305.2
Accession Version	XM_030243597.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2676bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	microtubule-associated protein 1S isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACAAA TTGATCTGGG AATGTTGTCA CAGGCCTGTT GTCCCAGCAG GAGGAGGCAG 
AAGCAGCTCC GGAACCTCCT GATGGACCCC GCCCCTCACA AGCTGCTGGT CCTGGCCGGG 
CCCTGCCTGG AGGAGACTGG GGAACTGCTG TTGCAGACTG GGGGGTTCTC GGCCCACCAC 
TTCCTGCAGG TGCTGGGGGA CAAAGAGGTC CAGGATGCAC TGGCCTCAGC ACCAGCTGCC 
CCAGCCCTCA CTGTGTCCTG TCCAACCTTT GGGGACTGGG CACTGTTGGG CCCTGTGCCC 
GGGCTGCAGC TGCGACTAAA TCCGCCCGCG CAGCTGCCGG CCTCAGAGGG CCTTCGTGCC 
TTCCTGGAGT ATGTGGCTGA GTCGCTGGAG CCGCCATCGC CCTTCGAGTT GTTGGAGCCA 
CCGGCTGCGG GCGGCTTTCT GCGCTTAGCA AGGCCCTGTT GCTATGTGTT CCCGGGTGGC 
TTGGGAGACG CCGCCTTCTT CGCTGTCAAC GGCTTTACAG TGCTGGTGAA TGGTGGCTCC 
AACCCCAAGT CGAGCTTCTG GAAGCTGGTG CGCCACCTGG ATCGCGTGGA CGCTGTCCTT 
GTGACCCACG CGGGTGCTGA CAGCTTGCCG GGCCTCAACA GCCTGCTGCG CCGCAAGCTG 
GCAGAGCGCG AAGCAGCCGC AGGACCACAG GGACAGCATG AGGAGCGGCT ACGTCGCCTG 
CTGTCGCCTG CGCTGGGTGT GGTGTTCCTG AATGCTCGTG AGGCTGCTTC TCGGTTGCGT 
GGGGGCGAAG ACGAGGCTGT GTGTGCTCGG AGCCTGCTGC GGAGCCTGGG CATTGCGCCT 
CTGCCACTGC AGCGTGGCCC ACAGCCCTCT TGCCCCACTG TCCTTTTCGA GAAGCTGGGC 
GTAGGCCGCC TAGAGCTTTT TGTGCTGCAC CCTCCTCCTG GGGACCCTGC AGCGCCTGCC 
TGTGCTCTGC TCGTGTGGCA GCCAGCGGCG CCTGGCGACA AGGTTGTGCG TGTACTATTT 
CCTGGCCGCA CGCCGCCCGC GCGCCTGCTG GATGGGCTAC AGCGCCTGCA GCACCTGCCG 
TGCCTGCGCA GGCCAGTAGT TACCACGCAT GACCTGGAAG CGCCCTCCAG GGCCAACAGC 
CAAGACAGTC TGGCCTCGCG GGACAGTGCA CGCAAGGAGC CAGTCCGTGG CACTGTTGGC 
AGCATTGCCA ACAGGAGTAC AGTGCGAAGG GAGCCTGCCC TGGCCACTCG TGACCAGAAG 
AAGGACACAA GATCTGGGCC CACTCAGCCT ACTGCCCGAG ACACACGCCG CTCCGGGCCT 
GGTGTGGTCA ACACAAAGCC TCGTGTATCA CAGAATGGGC CTCGTGCCCC AGTTCTGGCA 
GCGCCACTCA CAGCTCCTGT GGCTGAGTGC CCTGGAGAAG CGGAGAACAT CTTGGAGTCT 
GAGCGGCCAC CTGCCCCATC CCCAACTCTG TCCCCAGCTC AGTCCCCACC ACCTACAGCA 
CCTGGTAATA GCCCTGAGCG CCTGTCACTC AGCCCTCTGC GCCCAGAGCC TGCCCCTGAT 
GCGTCGCCCT CAGCCACGAC GCCCACGCTG ACCACGCCCT CTTTGCCTGC GGAGCTGGGC 
TCACCCCACT CAACTGAGGT GGACGAGTCG CTCTCTGTGT CTTTCGAGCA AGTCCTGCCA 
GCAGGTGACC CAGGGCTTAG CCTGCCCCTG CGCCTCGCGC GCCGCTCCAC GTCTCCTCAT 
GATGTGGACC TGTGCCTCGT GTCACCCTGT GAGTTCTCTC ATCGCAAGCC ACCCCCTCCT 
GCCTCCCCAG GCAGCTCGGA CAGCAGTGCT CGGTCACAGG AGCGGCCACC TGAGACTCCA 
CCCACGTCTG TGAGCGAGTC ACTGCCCACA CTATCTGACT CTGATCCCGT GCCCGTGGCG 
GATTCTGATG ATGACGCAGG CAGCGAGAGC GCGGCCAGGG ACCCGCCGCC TACACCTCGT 
GTGCCACCAC CACTGCCAGA TGTACCAGGA ATCTGCATGG TGGACCCGGA GGCGCTACCG 
CCTCGTGCCC GGCAGCCCCT CAACACCACC AATCCCAGCC GTAGCCGCAA GGCCCCTGCA 
AGGCCTAGCT CTGCCTCTGC CACCCCCAGA GCTGCAACTG TAGCTGCCAA GACAAAGGGC 
CCCGCGGGGG ACCGGAACCG GCCACTCAGC GCCCGCAGTG AGCCTGCAGA CAGGCCAGGC 
CGTGTGCCCC TTACCAGGAA GCCCTCAGTC CCTAAGACCG TTCCCAAGAT GGCCTCTGCC 
ACAAGGCTCT CCTCCGGACC CAGTGGCCGC CCTGCACCGC TTGCTGCTGG CTCCCCTGTA 
TACCTGGACC TGGCCTACCT GCCTGGTGGT GGTGCCGGTC ACCTGGACCA GAACTTCTTC 
CTGCGTGTCC GTGCACTCTG CTATGTCATC AGTGGGCAAG GCCAGCGCCA GGAAGAGGGC 
CTGCGAGCTG TGCTGGATGC ACTGCTGGCT GGCAAGCGGC AGTGGGACCT TGACTTGCAG 
GTCACACTGA TCCCCACCTT CGACTCTGCG GTGATGCACA GGTGGTATGA GGAGACGCAT 
GCGCAGCACC AGGCACTGGG CATCAGGGTG CTGGGCAGTG GCAGCCTGGT GTCCATGCAG 
GACGAGGCCT TCCCCGCCTG CAAGGTGGAG TTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030099457.1
CDS	607..3282
Translation	MSQIDLGMLSQACCPSRRRQKQLRNLLMDPAPHKLLVLAGPCLEETGELLLQTGGFSAHHFLQVLGDKEVQDALASAPAAPALTVSCPTFGDWALLGPVPGLQLRLNPPAQLPASEGLRAFLEYVAESLEPPSPFELLEPPAAGGFLRLARPCCYVFPGGLGDAAFFAVNGFTVLVNGGSNPKSSFWKLVRHLDRVDAVLVTHAGADSLPGLNSLLRRKLAEREAAAGPQGQHEERLRRLLSPALGVVFLNAREAASRLRGGEDEAVCARSLLRSLGIAPLPLQRGPQPSCPTVLFEKLGVGRLELFVLHPPPGDPAAPACALLVWQPAAPGDKVVRVLFPGRTPPARLLDGLQRLQHLPCLRRPVVTTHDLEAPSRANSQDSLASRDSARKEPVRGTVGSIANRSTVRREPALATRDQKKDTRSGPTQPTARDTRRSGPGVVNTKPRVSQNGPRAPVLAAPLTAPVAECPGEAENILESERPPAPSPTLSPAQSPPPTAPGNSPERLSLSPLRPEPAPDASPSATTPTLTTPSLPAELGSPHSTEVDESLSVSFEQVLPAGDPGLSLPLRLARRSTSPHDVDLCLVSPCEFSHRKPPPPASPGSSDSSARSQERPPETPPTSVSESLPTLSDSDPVPVADSDDDAGSESAARDPPPTPRVPPPLPDVPGICMVDPEALPPRARQPLNTTNPSRSRKAPARPSSASATPRAATVAAKTKGPAGDRNRPLSARSEPADRPGRVPLTRKPSVPKTVPKMASATRLSSGPSGRPAPLAAGSPVYLDLAYLPGGGAGHLDQNFFLRVRALCYVISGQGQRQEEGLRAVLDALLAGKRQWDLDLQVTLIPTFDSAVMHRWYEETHAQHQALGIRVLGSGSLVSMQDEAFPACKVEF

Target ORF information:

RefSeq Version	XM_030243597.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus microtubule-associated protein 1S (Map1s), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030243597.1

ORF Insert Sequence:

ATGTCACAAA TTGATCTGGG AATGTTGTCA CAGGCCTGTT GTCCCAGCAG GAGGAGGCAG 
AAGCAGCTCC GGAACCTCCT GATGGACCCC GCCCCTCACA AGCTGCTGGT CCTGGCCGGG 
CCCTGCCTGG AGGAGACTGG GGAACTGCTG TTGCAGACTG GGGGGTTCTC GGCCCACCAC 
TTCCTGCAGG TGCTGGGGGA CAAAGAGGTC CAGGATGCAC TGGCCTCAGC ACCAGCTGCC 
CCAGCCCTCA CTGTGTCCTG TCCAACCTTT GGGGACTGGG CACTGTTGGG CCCTGTGCCC 
GGGCTGCAGC TGCGACTAAA TCCGCCCGCG CAGCTGCCGG CCTCAGAGGG CCTTCGTGCC 
TTCCTGGAGT ATGTGGCTGA GTCGCTGGAG CCGCCATCGC CCTTCGAGTT GTTGGAGCCA 
CCGGCTGCGG GCGGCTTTCT GCGCTTAGCA AGGCCCTGTT GCTATGTGTT CCCGGGTGGC 
TTGGGAGACG CCGCCTTCTT CGCTGTCAAC GGCTTTACAG TGCTGGTGAA TGGTGGCTCC 
AACCCCAAGT CGAGCTTCTG GAAGCTGGTG CGCCACCTGG ATCGCGTGGA CGCTGTCCTT 
GTGACCCACG CGGGTGCTGA CAGCTTGCCG GGCCTCAACA GCCTGCTGCG CCGCAAGCTG 
GCAGAGCGCG AAGCAGCCGC AGGACCACAG GGACAGCATG AGGAGCGGCT ACGTCGCCTG 
CTGTCGCCTG CGCTGGGTGT GGTGTTCCTG AATGCTCGTG AGGCTGCTTC TCGGTTGCGT 
GGGGGCGAAG ACGAGGCTGT GTGTGCTCGG AGCCTGCTGC GGAGCCTGGG CATTGCGCCT 
CTGCCACTGC AGCGTGGCCC ACAGCCCTCT TGCCCCACTG TCCTTTTCGA GAAGCTGGGC 
GTAGGCCGCC TAGAGCTTTT TGTGCTGCAC CCTCCTCCTG GGGACCCTGC AGCGCCTGCC 
TGTGCTCTGC TCGTGTGGCA GCCAGCGGCG CCTGGCGACA AGGTTGTGCG TGTACTATTT 
CCTGGCCGCA CGCCGCCCGC GCGCCTGCTG GATGGGCTAC AGCGCCTGCA GCACCTGCCG 
TGCCTGCGCA GGCCAGTAGT TACCACGCAT GACCTGGAAG CGCCCTCCAG GGCCAACAGC 
CAAGACAGTC TGGCCTCGCG GGACAGTGCA CGCAAGGAGC CAGTCCGTGG CACTGTTGGC 
AGCATTGCCA ACAGGAGTAC AGTGCGAAGG GAGCCTGCCC TGGCCACTCG TGACCAGAAG 
AAGGACACAA GATCTGGGCC CACTCAGCCT ACTGCCCGAG ACACACGCCG CTCCGGGCCT 
GGTGTGGTCA ACACAAAGCC TCGTGTATCA CAGAATGGGC CTCGTGCCCC AGTTCTGGCA 
GCGCCACTCA CAGCTCCTGT GGCTGAGTGC CCTGGAGAAG CGGAGAACAT CTTGGAGTCT 
GAGCGGCCAC CTGCCCCATC CCCAACTCTG TCCCCAGCTC AGTCCCCACC ACCTACAGCA 
CCTGGTAATA GCCCTGAGCG CCTGTCACTC AGCCCTCTGC GCCCAGAGCC TGCCCCTGAT 
GCGTCGCCCT CAGCCACGAC GCCCACGCTG ACCACGCCCT CTTTGCCTGC GGAGCTGGGC 
TCACCCCACT CAACTGAGGT GGACGAGTCG CTCTCTGTGT CTTTCGAGCA AGTCCTGCCA 
GCAGGTGACC CAGGGCTTAG CCTGCCCCTG CGCCTCGCGC GCCGCTCCAC GTCTCCTCAT 
GATGTGGACC TGTGCCTCGT GTCACCCTGT GAGTTCTCTC ATCGCAAGCC ACCCCCTCCT 
GCCTCCCCAG GCAGCTCGGA CAGCAGTGCT CGGTCACAGG AGCGGCCACC TGAGACTCCA 
CCCACGTCTG TGAGCGAGTC ACTGCCCACA CTATCTGACT CTGATCCCGT GCCCGTGGCG 
GATTCTGATG ATGACGCAGG CAGCGAGAGC GCGGCCAGGG ACCCGCCGCC TACACCTCGT 
GTGCCACCAC CACTGCCAGA TGTACCAGGA ATCTGCATGG TGGACCCGGA GGCGCTACCG 
CCTCGTGCCC GGCAGCCCCT CAACACCACC AATCCCAGCC GTAGCCGCAA GGCCCCTGCA 
AGGCCTAGCT CTGCCTCTGC CACCCCCAGA GCTGCAACTG TAGCTGCCAA GACAAAGGGC 
CCCGCGGGGG ACCGGAACCG GCCACTCAGC GCCCGCAGTG AGCCTGCAGA CAGGCCAGGC 
CGTGTGCCCC TTACCAGGAA GCCCTCAGTC CCTAAGACCG TTCCCAAGAT GGCCTCTGCC 
ACAAGGCTCT CCTCCGGACC CAGTGGCCGC CCTGCACCGC TTGCTGCTGG CTCCCCTGTA 
TACCTGGACC TGGCCTACCT GCCTGGTGGT GGTGCCGGTC ACCTGGACCA GAACTTCTTC 
CTGCGTGTCC GTGCACTCTG CTATGTCATC AGTGGGCAAG GCCAGCGCCA GGAAGAGGGC 
CTGCGAGCTG TGCTGGATGC ACTGCTGGCT GGCAAGCGGC AGTGGGACCT TGACTTGCAG 
GTCACACTGA TCCCCACCTT CGACTCTGCG GTGATGCACA GGTGGTATGA GGAGACGCAT 
GCGCAGCACC AGGCACTGGG CATCAGGGTG CTGGGCAGTG GCAGCCTGGT GTCCATGCAG 
GACGAGGCCT TCCCCGCCTG CAAGGTGGAG TTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Spermidine Prolongs Lifespan and Prevents Liver Fibrosis and Hepatocellular Carcinoma by Activating MAP1S-Mediated Autophagy.
Cancer research77(11)2938-2951(2017 Jun)
Yue F,Li W,Zou J,Jiang X,Xu G,Huang H,Liu L

Defects in MAP1S-mediated autophagy turnover of fibronectin cause renal fibrosis.
Aging8(5)977-85(2016 May)
Xu G,Yue F,Huang H,He Y,Li X,Zhao H,Su Z,Jiang X,Li W,Zou J,Chen Q,Liu L

Defects in MAP1S-mediated autophagy cause reduction in mouse lifespans especially when fibronectin is overexpressed.
Aging cell15(2)370-9(2016 Apr)
Li W,Zou J,Yue F,Song K,Chen Q,McKeehan WL,Wang F,Xu G,Huang H,Yi J,Liu L

MAP1S Protein Regulates the Phagocytosis of Bacteria and Toll-like Receptor (TLR) Signaling.
The Journal of biological chemistry291(3)1243-50(2016 Jan)
Shi M,Zhang Y,Liu L,Zhang T,Han F,Cleveland J,Wang F,McKeehan WL,Li Y,Zhang D

MAP1S enhances autophagy to suppress tumorigenesis.
Autophagy8(2)278-80(2012 Feb)
Liu L,McKeehan WL,Wang F,Xie R

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Map1s cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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