Chsy1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Chsy1
Entrez Gene ID	269941
Full Name	chondroitin sulfate synthase 1
Synonyms	mKIAA0990
General protein information	Preferred Names chondroitin sulfate synthase 1 Names chondroitin sulfate synthase 1 N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase 1 N-acetylgalactosaminyltransferase 1 carbohydrate (chondroitin) synthase 1 chSy-1 chondroitin glucuronyltransferase 1 chondroitin synthase 1 glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001081163.1	NP_001074632.1	chondroitin sulfate synthase 1 precursor
XM_006540910.3	XP_006540973.1	chondroitin sulfate synthase 1 isoform X1
NM_001081163.2	NP_001074632.1	chondroitin sulfate synthase 1 precursor
XM_006540910.4	XP_006540973.1	chondroitin sulfate synthase 1 isoform X1

Pathways from BioSystems

Homologies

	CHSY1	XP_001084554.2
Rattus norvegicus (Norway rat)	Chsy1	NP_001099738.1
Homo sapiens (human)	CHSY1	NP_055733.2
Gallus gallus (chicken)	CHSY1	XP_413889.3
Drosophila melanogaster (fruit fly)	CG9220	NP_996440.2
Canis lupus familiaris (dog)	CHSY1	XP_545821.3
Danio rerio (zebrafish)	chsy1	NP_997843.1
Bos taurus (cattle)	CHSY1	NP_001178086.1
Mus musculus (house mouse)	Chsy1	NP_001074632.1
Xenopus tropicalis (tropical clawed frog)	chsy1	NP_001090724.1
Pan troglodytes (chimpanzee)	CHSY1	XP_523172.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001010	XP_309203.5

Gene Ontology
Bibliography

Related articles in PubMed

GlcUA?1-3Gal?1-3Gal?1-4Xyl(2-O-phosphate) is the preferred substrate for chondroitin N-acetylgalactosaminyltransferase-1.
Izumikawa T, Sato B, Mikami T, Tamura J, Igarashi M, Kitagawa H
The Journal of biological chemistry290(9)5438-48(2015 Feb)

Chondroitin sulfate synthase 1 (Chsy1) is required for bone development and digit patterning.
Wilson DG, Phamluong K, Lin WY, Barck K, Carano RA, Diehl L, Peterson AS, Martin F, Solloway MJ
Developmental biology363(2)413-25(2012 Mar)

Chondroitin sulfate synthase-2 is necessary for chain extension of chondroitin sulfate but not critical for skeletal development.
Ogawa H, Hatano S, Sugiura N, Nagai N, Sato T, Shimizu K, Kimata K, Narimatsu H, Watanabe H
PloS one7(8)e43806(2012)

A mouse knockout library for secreted and transmembrane proteins.
Tang T, Li L, Tang J, Li Y, Lin WY, Martin F, Grant D, Solloway M, Parker L, Ye W, Forrest W, Ghilardi N, Oravecz T, Platt KA, Rice DS, Hansen GM, Abuin A, Eberhart DE, Godowski P, Holt KH, Peterson A, Zambrowicz BP, de Sauvage FJ
Nature biotechnology28(7)749-55(2010 Jul)

Chondroitin sulfate N-acetylgalactosaminyltransferase-1 plays a critical role in chondroitin sulfate synthesis in cartilage.
Sakai K, Kimata K, Sato T, Gotoh M, Narimatsu H, Shinomiya K, Watanabe H
The Journal of biological chemistry282(6)4152-61(2007 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate a phosphorylated tetrasaccharide linkage structure, GlcUAbeta1-3Galbeta1-3Galbeta1-4Xyl(2-O-phosphate), in chondroitin N-acetylgalactosaminyltransferase-1 knockout ChGn-1(-/-) growth plate cartilage.
Title: GlcUA?1-3Gal?1-3Gal?1-4Xyl(2-O-phosphate) is the preferred substrate for chondroitin N-acetylgalactosaminyltransferase-1.

The results showed that Chsy1 is an essential regulator of joint patterning and provide a mouse model of human brachydactylies caused by mutations in CHSY1.
Title: Chondroitin sulfate synthase 1 (Chsy1) is required for bone development and digit patterning.

The following Chsy1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Chsy1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu38862	XM_006540910.4 Latest version!	Mus musculus chondroitin sulfate synthase 1 (Chsy1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu01796	NM_001081163.2 Latest version!	Mus musculus chondroitin sulfate synthase 1 (Chsy1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
View Old Accession Versions >>
OMu01796	NM_001081163.1	Mus musculus chondroitin sulfate synthase 1 (Chsy1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OMu38862	XM_006540910.3	Mus musculus chondroitin sulfate synthase 1 (Chsy1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Chsy1 ( XM_006540910.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006540973.1 Mus musculus chondroitin sulfate synthase 1 (Chsy1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu38862
Clone ID Related Accession (Same CDS sequence)	XM_006540910.3 , XM_006540910.4
Accession Version	XM_006540910.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	chondroitin sulfate synthase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006540910.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCTCA AGTACATGCA CGATCACTAC CTGGACAAGT ACGAGTGGTT TATGAGGGCA 
GACGATGACG TTTACATCAA GGGAGATCGT CTGGAGAGTT TCCTGAGGAG TTTGAATAGC 
AGCGAGCCCC TCTTCCTCGG GCAGACAGGA CTGGGCACCA CAGAAGAAAT GGGGAAGCTG 
GCCCTTGAGC CTGGAGAGAA CTTCTGCATG GGCGGGCCTG GTGTGATTCT GAGCCGGGAA 
GTGCTTCGGA GAATGGCACC ACACATTGGC AAGTGTCTCC GGGAAATGTA CACCACCCAT 
GAGGATGTAG AGGTGGGGAG ATGTGTCCGG AGATTCGCAG GCGTGCAGTG TGTCTGGTCT 
TATGAGATGC AGCAGCTTTT TTATGAGAAT TATGAACAGA ACAAGAAAGG GTACATTAGA 
GATCTCCATA GCAGTAAAAT CCACCGAGCG ATCACCTTAC ACCCCAACAA AAACCCACCG 
TACCAGTACA GGCTGCACAG TTACATGCTC AGCCGCAAGA TCGCCGAGCT GCGCCATCGC 
ACGATCCAGC TCCACCGGGA GATCGTCCTG ATGAGCAAGT ACAGCAGCAC GGAGATCCAG 
AAGGAAGACC TCCAGCTGGG CATCCCCCCC TCCTTCATGC GCTTCCAGGC CCGCCAGAGA 
GAGGAGATCC TGGAGTGGGA GTTCCTTACT GGGAAATACT TATACTCAGC CACAGATGGC 
CAGCCTCCCC GCAGAGGGAT GGACTCAGCA CAGAGGGAGG CCTTGGATGA CATAGTCATG 
CAAGTGATGG AGATGATCAA TGCCAACGCC AAGACTCGCG GGCGCATCAT CGACTTCAAG 
GAGATCCAGT ATGGCTACAG GCGTGTGAAC CCCATGTACG GGGCAGAGTA CATTCTGGAC 
CTGCTGCTCC TGTATAAGAA ACACAAAGGG AAGAAGATGA CGGTCCCTGT GAGGCGGCAC 
GCGTACCTGC AGCAGACCTT CAGCAAGATG CAGTTTGTGG AGCACGAGGA GCTGGATGCA 
CAAGAGCTGG CAGACAGAAT CAACCAGGAC TCTGGGTCCC TGTCATTCCT GTCCAATTCC 
CTGAAGAAGC TCGTTGCCTT CCAGCTTCCT GGATCCAAGA CGGAGCACAA AGAACCCAAA 
GAAAAGAAGA TCAACATTCT GATCCCGTTG TCTGGGCGCT TCGACATGTT TGTGAGGTTC 
ATGGGGAACT TTGAGAAGAC GTGTCTCATT CCAAATCTGA ACGTGAAGCT GGTCATTCTG 
CTTTTCAACT CTGACTCTAA CCCCGACAAG GCCAAACAGG TCGAGCTGAT GCGAGATTAC 
CGAGTCAAGT ACCCTAAGGC TGACATGCAG GTCCTGCCTG TGTCGGGAGG GTTTTCAAGA 
GCACTGGCCC TAGAAGTAGG CTCTTCCCAG TTTAACAATG AGTCTTTGCT CTTCTTCTGT 
GATGTGGACC TGGTGTTTAC AGCGGAATTT TTACAGAGGT GCCGAGCCAA CACAGTGCTG 
GGGCAGCAGA TCTATTTCCC AATCATCTTT AGCCAGTATG ATCCAAAGAT TGTTTATAGT 
GGGAAGGTTC CCAGTGACAA CCATTTTGCC TTTACTCAGA AAACAGGTTT CTGGAGAAAC 
TATGGGTTTG GCATCACTTG TATTTACAAG GGAGATCTTG TCAGAGTGGG TGGCTTCGAT 
GTCTCCATCC AAGGCTGGGG GCTGGAGGAT GTGGACCTCT TTAACAAGGT TGTCCAGGCA 
GGGTTGAAGA CATTCAGGAG CCAGGAGGTT GGAGTTGTTC ACATCCACCA CCCTGTCGTC 
TGTGACCCTA ATCTGGACCC CAAACAGTAT AAAATGTGCT TGGGGTCGAA AGCATCGACA 
TTTGGGTCCA CGCAGCAGCT GGCCGAAATG TGGCTAGAAA AGAATGACCC CAGTTACAGC 
AAAGGTGGCA GCCACGGCTC TGCCAGGACA GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006540973.1
CDS	386..2341
Translation	MMLKYMHDHYLDKYEWFMRADDDVYIKGDRLESFLRSLNSSEPLFLGQTGLGTTEEMGKLALEPGENFCMGGPGVILSREVLRRMAPHIGKCLREMYTTHEDVEVGRCVRRFAGVQCVWSYEMQQLFYENYEQNKKGYIRDLHSSKIHRAITLHPNKNPPYQYRLHSYMLSRKIAELRHRTIQLHREIVLMSKYSSTEIQKEDLQLGIPPSFMRFQARQREEILEWEFLTGKYLYSATDGQPPRRGMDSAQREALDDIVMQVMEMINANAKTRGRIIDFKEIQYGYRRVNPMYGAEYILDLLLLYKKHKGKKMTVPVRRHAYLQQTFSKMQFVEHEELDAQELADRINQDSGSLSFLSNSLKKLVAFQLPGSKTEHKEPKEKKINILIPLSGRFDMFVRFMGNFEKTCLIPNLNVKLVILLFNSDSNPDKAKQVELMRDYRVKYPKADMQVLPVSGGFSRALALEVGSSQFNNESLLFFCDVDLVFTAEFLQRCRANTVLGQQIYFPIIFSQYDPKIVYSGKVPSDNHFAFTQKTGFWRNYGFGITCIYKGDLVRVGGFDVSIQGWGLEDVDLFNKVVQAGLKTFRSQEVGVVHIHHPVVCDPNLDPKQYKMCLGSKASTFGSTQQLAEMWLEKNDPSYSKGGSHGSARTA

Target ORF information:

RefSeq Version	XM_006540910.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus chondroitin sulfate synthase 1 (Chsy1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006540910.3

ORF Insert Sequence:

ATGATGCTCA AGTACATGCA CGATCACTAC CTGGACAAGT ACGAGTGGTT TATGAGGGCA 
GACGATGACG TTTACATCAA GGGAGATCGT CTGGAGAGTT TCCTGAGGAG TTTGAATAGC 
AGCGAGCCCC TCTTCCTCGG GCAGACAGGA CTGGGCACCA CAGAAGAAAT GGGGAAGCTG 
GCCCTTGAGC CTGGAGAGAA CTTCTGCATG GGCGGGCCTG GTGTGATTCT GAGCCGGGAA 
GTGCTTCGGA GAATGGCACC ACACATTGGC AAGTGTCTCC GGGAAATGTA CACCACCCAT 
GAGGATGTAG AGGTGGGGAG ATGTGTCCGG AGATTCGCAG GCGTGCAGTG TGTCTGGTCT 
TATGAGATGC AGCAGCTTTT TTATGAGAAT TATGAACAGA ACAAGAAAGG GTACATTAGA 
GATCTCCATA GCAGTAAAAT CCACCGAGCG ATCACCTTAC ACCCCAACAA AAACCCACCG 
TACCAGTACA GGCTGCACAG TTACATGCTC AGCCGCAAGA TCGCCGAGCT GCGCCATCGC 
ACGATCCAGC TCCACCGGGA GATCGTCCTG ATGAGCAAGT ACAGCAGCAC GGAGATCCAG 
AAGGAAGACC TCCAGCTGGG CATCCCCCCC TCCTTCATGC GCTTCCAGGC CCGCCAGAGA 
GAGGAGATCC TGGAGTGGGA GTTCCTTACT GGGAAATACT TATACTCAGC CACAGATGGC 
CAGCCTCCCC GCAGAGGGAT GGACTCAGCA CAGAGGGAGG CCTTGGATGA CATAGTCATG 
CAAGTGATGG AGATGATCAA TGCCAACGCC AAGACTCGCG GGCGCATCAT CGACTTCAAG 
GAGATCCAGT ATGGCTACAG GCGTGTGAAC CCCATGTACG GGGCAGAGTA CATTCTGGAC 
CTGCTGCTCC TGTATAAGAA ACACAAAGGG AAGAAGATGA CGGTCCCTGT GAGGCGGCAC 
GCGTACCTGC AGCAGACCTT CAGCAAGATG CAGTTTGTGG AGCACGAGGA GCTGGATGCA 
CAAGAGCTGG CAGACAGAAT CAACCAGGAC TCTGGGTCCC TGTCATTCCT GTCCAATTCC 
CTGAAGAAGC TCGTTGCCTT CCAGCTTCCT GGATCCAAGA CGGAGCACAA AGAACCCAAA 
GAAAAGAAGA TCAACATTCT GATCCCGTTG TCTGGGCGCT TCGACATGTT TGTGAGGTTC 
ATGGGGAACT TTGAGAAGAC GTGTCTCATT CCAAATCTGA ACGTGAAGCT GGTCATTCTG 
CTTTTCAACT CTGACTCTAA CCCCGACAAG GCCAAACAGG TCGAGCTGAT GCGAGATTAC 
CGAGTCAAGT ACCCTAAGGC TGACATGCAG GTCCTGCCTG TGTCGGGAGG GTTTTCAAGA 
GCACTGGCCC TAGAAGTAGG CTCTTCCCAG TTTAACAATG AGTCTTTGCT CTTCTTCTGT 
GATGTGGACC TGGTGTTTAC AGCGGAATTT TTACAGAGGT GCCGAGCCAA CACAGTGCTG 
GGGCAGCAGA TCTATTTCCC AATCATCTTT AGCCAGTATG ATCCAAAGAT TGTTTATAGT 
GGGAAGGTTC CCAGTGACAA CCATTTTGCC TTTACTCAGA AAACAGGTTT CTGGAGAAAC 
TATGGGTTTG GCATCACTTG TATTTACAAG GGAGATCTTG TCAGAGTGGG TGGCTTCGAT 
GTCTCCATCC AAGGCTGGGG GCTGGAGGAT GTGGACCTCT TTAACAAGGT TGTCCAGGCA 
GGGTTGAAGA CATTCAGGAG CCAGGAGGTT GGAGTTGTTC ACATCCACCA CCCTGTCGTC 
TGTGACCCTA ATCTGGACCC CAAACAGTAT AAAATGTGCT TGGGGTCGAA AGCATCGACA 
TTTGGGTCCA CGCAGCAGCT GGCCGAAATG TGGCTAGAAA AGAATGACCC CAGTTACAGC 
AAAGGTGGCA GCCACGGCTC TGCCAGGACA GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chsy1 ( XM_006540910.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006540973.1 Mus musculus chondroitin sulfate synthase 1 (Chsy1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu38862
Clone ID Related Accession (Same CDS sequence)	XM_006540910.3 , XM_006540910.4
Accession Version	XM_006540910.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	chondroitin sulfate synthase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006540910.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCTCA AGTACATGCA CGATCACTAC CTGGACAAGT ACGAGTGGTT TATGAGGGCA 
GACGATGACG TTTACATCAA GGGAGATCGT CTGGAGAGTT TCCTGAGGAG TTTGAATAGC 
AGCGAGCCCC TCTTCCTCGG GCAGACAGGA CTGGGCACCA CAGAAGAAAT GGGGAAGCTG 
GCCCTTGAGC CTGGAGAGAA CTTCTGCATG GGCGGGCCTG GTGTGATTCT GAGCCGGGAA 
GTGCTTCGGA GAATGGCACC ACACATTGGC AAGTGTCTCC GGGAAATGTA CACCACCCAT 
GAGGATGTAG AGGTGGGGAG ATGTGTCCGG AGATTCGCAG GCGTGCAGTG TGTCTGGTCT 
TATGAGATGC AGCAGCTTTT TTATGAGAAT TATGAACAGA ACAAGAAAGG GTACATTAGA 
GATCTCCATA GCAGTAAAAT CCACCGAGCG ATCACCTTAC ACCCCAACAA AAACCCACCG 
TACCAGTACA GGCTGCACAG TTACATGCTC AGCCGCAAGA TCGCCGAGCT GCGCCATCGC 
ACGATCCAGC TCCACCGGGA GATCGTCCTG ATGAGCAAGT ACAGCAGCAC GGAGATCCAG 
AAGGAAGACC TCCAGCTGGG CATCCCCCCC TCCTTCATGC GCTTCCAGGC CCGCCAGAGA 
GAGGAGATCC TGGAGTGGGA GTTCCTTACT GGGAAATACT TATACTCAGC CACAGATGGC 
CAGCCTCCCC GCAGAGGGAT GGACTCAGCA CAGAGGGAGG CCTTGGATGA CATAGTCATG 
CAAGTGATGG AGATGATCAA TGCCAACGCC AAGACTCGCG GGCGCATCAT CGACTTCAAG 
GAGATCCAGT ATGGCTACAG GCGTGTGAAC CCCATGTACG GGGCAGAGTA CATTCTGGAC 
CTGCTGCTCC TGTATAAGAA ACACAAAGGG AAGAAGATGA CGGTCCCTGT GAGGCGGCAC 
GCGTACCTGC AGCAGACCTT CAGCAAGATG CAGTTTGTGG AGCACGAGGA GCTGGATGCA 
CAAGAGCTGG CAGACAGAAT CAACCAGGAC TCTGGGTCCC TGTCATTCCT GTCCAATTCC 
CTGAAGAAGC TCGTTGCCTT CCAGCTTCCT GGATCCAAGA CGGAGCACAA AGAACCCAAA 
GAAAAGAAGA TCAACATTCT GATCCCGTTG TCTGGGCGCT TCGACATGTT TGTGAGGTTC 
ATGGGGAACT TTGAGAAGAC GTGTCTCATT CCAAATCTGA ACGTGAAGCT GGTCATTCTG 
CTTTTCAACT CTGACTCTAA CCCCGACAAG GCCAAACAGG TCGAGCTGAT GCGAGATTAC 
CGAGTCAAGT ACCCTAAGGC TGACATGCAG GTCCTGCCTG TGTCGGGAGG GTTTTCAAGA 
GCACTGGCCC TAGAAGTAGG CTCTTCCCAG TTTAACAATG AGTCTTTGCT CTTCTTCTGT 
GATGTGGACC TGGTGTTTAC AGCGGAATTT TTACAGAGGT GCCGAGCCAA CACAGTGCTG 
GGGCAGCAGA TCTATTTCCC AATCATCTTT AGCCAGTATG ATCCAAAGAT TGTTTATAGT 
GGGAAGGTTC CCAGTGACAA CCATTTTGCC TTTACTCAGA AAACAGGTTT CTGGAGAAAC 
TATGGGTTTG GCATCACTTG TATTTACAAG GGAGATCTTG TCAGAGTGGG TGGCTTCGAT 
GTCTCCATCC AAGGCTGGGG GCTGGAGGAT GTGGACCTCT TTAACAAGGT TGTCCAGGCA 
GGGTTGAAGA CATTCAGGAG CCAGGAGGTT GGAGTTGTTC ACATCCACCA CCCTGTCGTC 
TGTGACCCTA ATCTGGACCC CAAACAGTAT AAAATGTGCT TGGGGTCGAA AGCATCGACA 
TTTGGGTCCA CGCAGCAGCT GGCCGAAATG TGGCTAGAAA AGAATGACCC CAGTTACAGC 
AAAGGTGGCA GCCACGGCTC TGCCAGGACA GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006540973.1
CDS	384..2339
Translation	MMLKYMHDHYLDKYEWFMRADDDVYIKGDRLESFLRSLNSSEPLFLGQTGLGTTEEMGKLALEPGENFCMGGPGVILSREVLRRMAPHIGKCLREMYTTHEDVEVGRCVRRFAGVQCVWSYEMQQLFYENYEQNKKGYIRDLHSSKIHRAITLHPNKNPPYQYRLHSYMLSRKIAELRHRTIQLHREIVLMSKYSSTEIQKEDLQLGIPPSFMRFQARQREEILEWEFLTGKYLYSATDGQPPRRGMDSAQREALDDIVMQVMEMINANAKTRGRIIDFKEIQYGYRRVNPMYGAEYILDLLLLYKKHKGKKMTVPVRRHAYLQQTFSKMQFVEHEELDAQELADRINQDSGSLSFLSNSLKKLVAFQLPGSKTEHKEPKEKKINILIPLSGRFDMFVRFMGNFEKTCLIPNLNVKLVILLFNSDSNPDKAKQVELMRDYRVKYPKADMQVLPVSGGFSRALALEVGSSQFNNESLLFFCDVDLVFTAEFLQRCRANTVLGQQIYFPIIFSQYDPKIVYSGKVPSDNHFAFTQKTGFWRNYGFGITCIYKGDLVRVGGFDVSIQGWGLEDVDLFNKVVQAGLKTFRSQEVGVVHIHHPVVCDPNLDPKQYKMCLGSKASTFGSTQQLAEMWLEKNDPSYSKGGSHGSARTA

Target ORF information:

RefSeq Version	XM_006540910.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus chondroitin sulfate synthase 1 (Chsy1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006540910.4

ORF Insert Sequence:

ATGATGCTCA AGTACATGCA CGATCACTAC CTGGACAAGT ACGAGTGGTT TATGAGGGCA 
GACGATGACG TTTACATCAA GGGAGATCGT CTGGAGAGTT TCCTGAGGAG TTTGAATAGC 
AGCGAGCCCC TCTTCCTCGG GCAGACAGGA CTGGGCACCA CAGAAGAAAT GGGGAAGCTG 
GCCCTTGAGC CTGGAGAGAA CTTCTGCATG GGCGGGCCTG GTGTGATTCT GAGCCGGGAA 
GTGCTTCGGA GAATGGCACC ACACATTGGC AAGTGTCTCC GGGAAATGTA CACCACCCAT 
GAGGATGTAG AGGTGGGGAG ATGTGTCCGG AGATTCGCAG GCGTGCAGTG TGTCTGGTCT 
TATGAGATGC AGCAGCTTTT TTATGAGAAT TATGAACAGA ACAAGAAAGG GTACATTAGA 
GATCTCCATA GCAGTAAAAT CCACCGAGCG ATCACCTTAC ACCCCAACAA AAACCCACCG 
TACCAGTACA GGCTGCACAG TTACATGCTC AGCCGCAAGA TCGCCGAGCT GCGCCATCGC 
ACGATCCAGC TCCACCGGGA GATCGTCCTG ATGAGCAAGT ACAGCAGCAC GGAGATCCAG 
AAGGAAGACC TCCAGCTGGG CATCCCCCCC TCCTTCATGC GCTTCCAGGC CCGCCAGAGA 
GAGGAGATCC TGGAGTGGGA GTTCCTTACT GGGAAATACT TATACTCAGC CACAGATGGC 
CAGCCTCCCC GCAGAGGGAT GGACTCAGCA CAGAGGGAGG CCTTGGATGA CATAGTCATG 
CAAGTGATGG AGATGATCAA TGCCAACGCC AAGACTCGCG GGCGCATCAT CGACTTCAAG 
GAGATCCAGT ATGGCTACAG GCGTGTGAAC CCCATGTACG GGGCAGAGTA CATTCTGGAC 
CTGCTGCTCC TGTATAAGAA ACACAAAGGG AAGAAGATGA CGGTCCCTGT GAGGCGGCAC 
GCGTACCTGC AGCAGACCTT CAGCAAGATG CAGTTTGTGG AGCACGAGGA GCTGGATGCA 
CAAGAGCTGG CAGACAGAAT CAACCAGGAC TCTGGGTCCC TGTCATTCCT GTCCAATTCC 
CTGAAGAAGC TCGTTGCCTT CCAGCTTCCT GGATCCAAGA CGGAGCACAA AGAACCCAAA 
GAAAAGAAGA TCAACATTCT GATCCCGTTG TCTGGGCGCT TCGACATGTT TGTGAGGTTC 
ATGGGGAACT TTGAGAAGAC GTGTCTCATT CCAAATCTGA ACGTGAAGCT GGTCATTCTG 
CTTTTCAACT CTGACTCTAA CCCCGACAAG GCCAAACAGG TCGAGCTGAT GCGAGATTAC 
CGAGTCAAGT ACCCTAAGGC TGACATGCAG GTCCTGCCTG TGTCGGGAGG GTTTTCAAGA 
GCACTGGCCC TAGAAGTAGG CTCTTCCCAG TTTAACAATG AGTCTTTGCT CTTCTTCTGT 
GATGTGGACC TGGTGTTTAC AGCGGAATTT TTACAGAGGT GCCGAGCCAA CACAGTGCTG 
GGGCAGCAGA TCTATTTCCC AATCATCTTT AGCCAGTATG ATCCAAAGAT TGTTTATAGT 
GGGAAGGTTC CCAGTGACAA CCATTTTGCC TTTACTCAGA AAACAGGTTT CTGGAGAAAC 
TATGGGTTTG GCATCACTTG TATTTACAAG GGAGATCTTG TCAGAGTGGG TGGCTTCGAT 
GTCTCCATCC AAGGCTGGGG GCTGGAGGAT GTGGACCTCT TTAACAAGGT TGTCCAGGCA 
GGGTTGAAGA CATTCAGGAG CCAGGAGGTT GGAGTTGTTC ACATCCACCA CCCTGTCGTC 
TGTGACCCTA ATCTGGACCC CAAACAGTAT AAAATGTGCT TGGGGTCGAA AGCATCGACA 
TTTGGGTCCA CGCAGCAGCT GGCCGAAATG TGGCTAGAAA AGAATGACCC CAGTTACAGC 
AAAGGTGGCA GCCACGGCTC TGCCAGGACA GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chsy1 ( NM_001081163.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001074632.1 Mus musculus chondroitin sulfate synthase 1 (Chsy1), mRNA.

Price & Availability↑

CloneID	OMu01796
Clone ID Related Accession (Same CDS sequence)	NM_001081163.1 , NM_001081163.2
Accession Version	NM_001081163.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2403bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-19
Organism	Mus musculus(house mouse)
Product	chondroitin sulfate synthase 1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC124695.4 and AC127595.3. On Feb 6, 2007 this sequence version replaced XM_194358.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK129255.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCCGCGC GCGGCCGGCG CGCCTGGCTC AGCATGCTGC TCGGGCTCGT GCTGGGCTTC 
GTGCTGGCCT CGCGGCTCGT CCTGCCCCGC GCCTCGGAGC TGAAGCGAGT GGGCCCGCGG 
CGGCGCCCCA GTCCCGAGGG ATGCCGGCCA GGGCAGGAGG CGTCGCAGCC CGGCGGGGCG 
CGCGGCGATG CGCGCGGGGC GCAGCTCTGG CCGCAGGGCT CGGCCGCGGA GGGCGTCCCG 
CGCGACAGGA ACTTTCTCTT CGTGGGAGTC ATGACAGCCC AGAAATACCT GCAGACCCGC 
GCCGTGGCCG CCTACAGAAC ATGGTCCAAG ACAATTCCCG GGAAGGTGGA GTTCTTCTCT 
AGCGAGGGTT CAGATACATC TATACCCATT CCAGTGGTGC CCCTGCGGGG TGTGGATGAC 
TCCTACCCAC CCCAGAAGAA GTCTTTCATG ATGCTCAAGT ACATGCACGA TCACTACCTG 
GACAAGTACG AGTGGTTTAT GAGGGCAGAC GATGACGTTT ACATCAAGGG AGATCGTCTG 
GAGAGTTTCC TGAGGAGTTT GAATAGCAGC GAGCCCCTCT TCCTCGGGCA GACAGGACTG 
GGCACCACAG AAGAAATGGG GAAGCTGGCC CTTGAGCCTG GAGAGAACTT CTGCATGGGC 
GGGCCTGGTG TGATTCTGAG CCGGGAAGTG CTTCGGAGAA TGGCACCACA CATTGGCAAG 
TGTCTCCGGG AAATGTACAC CACCCATGAG GATGTAGAGG TGGGGAGATG TGTCCGGAGA 
TTCGCAGGCG TGCAGTGTGT CTGGTCTTAT GAGATGCAGC AGCTTTTTTA TGAGAATTAT 
GAACAGAACA AGAAAGGGTA CATTAGAGAT CTCCATAGCA GTAAAATCCA CCGAGCGATC 
ACCTTACACC CCAACAAAAA CCCACCGTAC CAGTACAGGC TGCACAGTTA CATGCTCAGC 
CGCAAGATCG CCGAGCTGCG CCATCGCACG ATCCAGCTCC ACCGGGAGAT CGTCCTGATG 
AGCAAGTACA GCAGCACGGA GATCCAGAAG GAAGACCTCC AGCTGGGCAT CCCCCCCTCC 
TTCATGCGCT TCCAGGCCCG CCAGAGAGAG GAGATCCTGG AGTGGGAGTT CCTTACTGGG 
AAATACTTAT ACTCAGCCAC AGATGGCCAG CCTCCCCGCA GAGGGATGGA CTCAGCACAG 
AGGGAGGCCT TGGATGACAT AGTCATGCAA GTGATGGAGA TGATCAATGC CAACGCCAAG 
ACTCGCGGGC GCATCATCGA CTTCAAGGAG ATCCAGTATG GCTACAGGCG TGTGAACCCC 
ATGTACGGGG CAGAGTACAT TCTGGACCTG CTGCTCCTGT ATAAGAAACA CAAAGGGAAG 
AAGATGACGG TCCCTGTGAG GCGGCACGCG TACCTGCAGC AGACCTTCAG CAAGATGCAG 
TTTGTGGAGC ACGAGGAGCT GGATGCACAA GAGCTGGCAG ACAGAATCAA CCAGGACTCT 
GGGTCCCTGT CATTCCTGTC CAATTCCCTG AAGAAGCTCG TTGCCTTCCA GCTTCCTGGA 
TCCAAGACGG AGCACAAAGA ACCCAAAGAA AAGAAGATCA ACATTCTGAT CCCGTTGTCT 
GGGCGCTTCG ACATGTTTGT GAGGTTCATG GGGAACTTTG AGAAGACGTG TCTCATTCCA 
AATCTGAACG TGAAGCTGGT CATTCTGCTT TTCAACTCTG ACTCTAACCC CGACAAGGCC 
AAACAGGTCG AGCTGATGCG AGATTACCGA GTCAAGTACC CTAAGGCTGA CATGCAGGTC 
CTGCCTGTGT CGGGAGGGTT TTCAAGAGCA CTGGCCCTAG AAGTAGGCTC TTCCCAGTTT 
AACAATGAGT CTTTGCTCTT CTTCTGTGAT GTGGACCTGG TGTTTACAGC GGAATTTTTA 
CAGAGGTGCC GAGCCAACAC AGTGCTGGGG CAGCAGATCT ATTTCCCAAT CATCTTTAGC 
CAGTATGATC CAAAGATTGT TTATAGTGGG AAGGTTCCCA GTGACAACCA TTTTGCCTTT 
ACTCAGAAAA CAGGTTTCTG GAGAAACTAT GGGTTTGGCA TCACTTGTAT TTACAAGGGA 
GATCTTGTCA GAGTGGGTGG CTTCGATGTC TCCATCCAAG GCTGGGGGCT GGAGGATGTG 
GACCTCTTTA ACAAGGTTGT CCAGGCAGGG TTGAAGACAT TCAGGAGCCA GGAGGTTGGA 
GTTGTTCACA TCCACCACCC TGTCGTCTGT GACCCTAATC TGGACCCCAA ACAGTATAAA 
ATGTGCTTGG GGTCGAAAGC ATCGACATTT GGGTCCACGC AGCAGCTGGC CGAAATGTGG 
CTAGAAAAGA ATGACCCCAG TTACAGCAAA GGTGGCAGCC ACGGCTCTGC CAGGACAGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074632.1
CDS	397..2799
Translation	MAARGRRAWLSMLLGLVLGFVLASRLVLPRASELKRVGPRRRPSPEGCRPGQEASQPGGARGDARGAQLWPQGSAAEGVPRDRNFLFVGVMTAQKYLQTRAVAAYRTWSKTIPGKVEFFSSEGSDTSIPIPVVPLRGVDDSYPPQKKSFMMLKYMHDHYLDKYEWFMRADDDVYIKGDRLESFLRSLNSSEPLFLGQTGLGTTEEMGKLALEPGENFCMGGPGVILSREVLRRMAPHIGKCLREMYTTHEDVEVGRCVRRFAGVQCVWSYEMQQLFYENYEQNKKGYIRDLHSSKIHRAITLHPNKNPPYQYRLHSYMLSRKIAELRHRTIQLHREIVLMSKYSSTEIQKEDLQLGIPPSFMRFQARQREEILEWEFLTGKYLYSATDGQPPRRGMDSAQREALDDIVMQVMEMINANAKTRGRIIDFKEIQYGYRRVNPMYGAEYILDLLLLYKKHKGKKMTVPVRRHAYLQQTFSKMQFVEHEELDAQELADRINQDSGSLSFLSNSLKKLVAFQLPGSKTEHKEPKEKKINILIPLSGRFDMFVRFMGNFEKTCLIPNLNVKLVILLFNSDSNPDKAKQVELMRDYRVKYPKADMQVLPVSGGFSRALALEVGSSQFNNESLLFFCDVDLVFTAEFLQRCRANTVLGQQIYFPIIFSQYDPKIVYSGKVPSDNHFAFTQKTGFWRNYGFGITCIYKGDLVRVGGFDVSIQGWGLEDVDLFNKVVQAGLKTFRSQEVGVVHIHHPVVCDPNLDPKQYKMCLGSKASTFGSTQQLAEMWLEKNDPSYSKGGSHGSARTA

Target ORF information:

RefSeq Version	NM_001081163.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus chondroitin sulfate synthase 1 (Chsy1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001081163.1

ORF Insert Sequence:

ATGGCCGCGC GCGGCCGGCG CGCCTGGCTC AGCATGCTGC TCGGGCTCGT GCTGGGCTTC 
GTGCTGGCCT CGCGGCTCGT CCTGCCCCGC GCCTCGGAGC TGAAGCGAGT GGGCCCGCGG 
CGGCGCCCCA GTCCCGAGGG ATGCCGGCCA GGGCAGGAGG CGTCGCAGCC CGGCGGGGCG 
CGCGGCGATG CGCGCGGGGC GCAGCTCTGG CCGCAGGGCT CGGCCGCGGA GGGCGTCCCG 
CGCGACAGGA ACTTTCTCTT CGTGGGAGTC ATGACAGCCC AGAAATACCT GCAGACCCGC 
GCCGTGGCCG CCTACAGAAC ATGGTCCAAG ACAATTCCCG GGAAGGTGGA GTTCTTCTCT 
AGCGAGGGTT CAGATACATC TATACCCATT CCAGTGGTGC CCCTGCGGGG TGTGGATGAC 
TCCTACCCAC CCCAGAAGAA GTCTTTCATG ATGCTCAAGT ACATGCACGA TCACTACCTG 
GACAAGTACG AGTGGTTTAT GAGGGCAGAC GATGACGTTT ACATCAAGGG AGATCGTCTG 
GAGAGTTTCC TGAGGAGTTT GAATAGCAGC GAGCCCCTCT TCCTCGGGCA GACAGGACTG 
GGCACCACAG AAGAAATGGG GAAGCTGGCC CTTGAGCCTG GAGAGAACTT CTGCATGGGC 
GGGCCTGGTG TGATTCTGAG CCGGGAAGTG CTTCGGAGAA TGGCACCACA CATTGGCAAG 
TGTCTCCGGG AAATGTACAC CACCCATGAG GATGTAGAGG TGGGGAGATG TGTCCGGAGA 
TTCGCAGGCG TGCAGTGTGT CTGGTCTTAT GAGATGCAGC AGCTTTTTTA TGAGAATTAT 
GAACAGAACA AGAAAGGGTA CATTAGAGAT CTCCATAGCA GTAAAATCCA CCGAGCGATC 
ACCTTACACC CCAACAAAAA CCCACCGTAC CAGTACAGGC TGCACAGTTA CATGCTCAGC 
CGCAAGATCG CCGAGCTGCG CCATCGCACG ATCCAGCTCC ACCGGGAGAT CGTCCTGATG 
AGCAAGTACA GCAGCACGGA GATCCAGAAG GAAGACCTCC AGCTGGGCAT CCCCCCCTCC 
TTCATGCGCT TCCAGGCCCG CCAGAGAGAG GAGATCCTGG AGTGGGAGTT CCTTACTGGG 
AAATACTTAT ACTCAGCCAC AGATGGCCAG CCTCCCCGCA GAGGGATGGA CTCAGCACAG 
AGGGAGGCCT TGGATGACAT AGTCATGCAA GTGATGGAGA TGATCAATGC CAACGCCAAG 
ACTCGCGGGC GCATCATCGA CTTCAAGGAG ATCCAGTATG GCTACAGGCG TGTGAACCCC 
ATGTACGGGG CAGAGTACAT TCTGGACCTG CTGCTCCTGT ATAAGAAACA CAAAGGGAAG 
AAGATGACGG TCCCTGTGAG GCGGCACGCG TACCTGCAGC AGACCTTCAG CAAGATGCAG 
TTTGTGGAGC ACGAGGAGCT GGATGCACAA GAGCTGGCAG ACAGAATCAA CCAGGACTCT 
GGGTCCCTGT CATTCCTGTC CAATTCCCTG AAGAAGCTCG TTGCCTTCCA GCTTCCTGGA 
TCCAAGACGG AGCACAAAGA ACCCAAAGAA AAGAAGATCA ACATTCTGAT CCCGTTGTCT 
GGGCGCTTCG ACATGTTTGT GAGGTTCATG GGGAACTTTG AGAAGACGTG TCTCATTCCA 
AATCTGAACG TGAAGCTGGT CATTCTGCTT TTCAACTCTG ACTCTAACCC CGACAAGGCC 
AAACAGGTCG AGCTGATGCG AGATTACCGA GTCAAGTACC CTAAGGCTGA CATGCAGGTC 
CTGCCTGTGT CGGGAGGGTT TTCAAGAGCA CTGGCCCTAG AAGTAGGCTC TTCCCAGTTT 
AACAATGAGT CTTTGCTCTT CTTCTGTGAT GTGGACCTGG TGTTTACAGC GGAATTTTTA 
CAGAGGTGCC GAGCCAACAC AGTGCTGGGG CAGCAGATCT ATTTCCCAAT CATCTTTAGC 
CAGTATGATC CAAAGATTGT TTATAGTGGG AAGGTTCCCA GTGACAACCA TTTTGCCTTT 
ACTCAGAAAA CAGGTTTCTG GAGAAACTAT GGGTTTGGCA TCACTTGTAT TTACAAGGGA 
GATCTTGTCA GAGTGGGTGG CTTCGATGTC TCCATCCAAG GCTGGGGGCT GGAGGATGTG 
GACCTCTTTA ACAAGGTTGT CCAGGCAGGG TTGAAGACAT TCAGGAGCCA GGAGGTTGGA 
GTTGTTCACA TCCACCACCC TGTCGTCTGT GACCCTAATC TGGACCCCAA ACAGTATAAA 
ATGTGCTTGG GGTCGAAAGC ATCGACATTT GGGTCCACGC AGCAGCTGGC CGAAATGTGG 
CTAGAAAAGA ATGACCCCAG TTACAGCAAA GGTGGCAGCC ACGGCTCTGC CAGGACAGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chsy1 ( NM_001081163.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001074632.1 Mus musculus chondroitin sulfate synthase 1 (Chsy1), mRNA.

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CloneID	OMu01796
Clone ID Related Accession (Same CDS sequence)	NM_001081163.1 , NM_001081163.2
Accession Version	NM_001081163.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2403bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-27
Organism	Mus musculus(house mouse)
Product	chondroitin sulfate synthase 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC124695.4 and AC127595.3. On Jun 10, 2018 this sequence version replaced NM_001081163.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK129255.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCGCGC GCGGCCGGCG CGCCTGGCTC AGCATGCTGC TCGGGCTCGT GCTGGGCTTC 
GTGCTGGCCT CGCGGCTCGT CCTGCCCCGC GCCTCGGAGC TGAAGCGAGT GGGCCCGCGG 
CGGCGCCCCA GTCCCGAGGG ATGCCGGCCA GGGCAGGAGG CGTCGCAGCC CGGCGGGGCG 
CGCGGCGATG CGCGCGGGGC GCAGCTCTGG CCGCAGGGCT CGGCCGCGGA GGGCGTCCCG 
CGCGACAGGA ACTTTCTCTT CGTGGGAGTC ATGACAGCCC AGAAATACCT GCAGACCCGC 
GCCGTGGCCG CCTACAGAAC ATGGTCCAAG ACAATTCCCG GGAAGGTGGA GTTCTTCTCT 
AGCGAGGGTT CAGATACATC TATACCCATT CCAGTGGTGC CCCTGCGGGG TGTGGATGAC 
TCCTACCCAC CCCAGAAGAA GTCTTTCATG ATGCTCAAGT ACATGCACGA TCACTACCTG 
GACAAGTACG AGTGGTTTAT GAGGGCAGAC GATGACGTTT ACATCAAGGG AGATCGTCTG 
GAGAGTTTCC TGAGGAGTTT GAATAGCAGC GAGCCCCTCT TCCTCGGGCA GACAGGACTG 
GGCACCACAG AAGAAATGGG GAAGCTGGCC CTTGAGCCTG GAGAGAACTT CTGCATGGGC 
GGGCCTGGTG TGATTCTGAG CCGGGAAGTG CTTCGGAGAA TGGCACCACA CATTGGCAAG 
TGTCTCCGGG AAATGTACAC CACCCATGAG GATGTAGAGG TGGGGAGATG TGTCCGGAGA 
TTCGCAGGCG TGCAGTGTGT CTGGTCTTAT GAGATGCAGC AGCTTTTTTA TGAGAATTAT 
GAACAGAACA AGAAAGGGTA CATTAGAGAT CTCCATAGCA GTAAAATCCA CCGAGCGATC 
ACCTTACACC CCAACAAAAA CCCACCGTAC CAGTACAGGC TGCACAGTTA CATGCTCAGC 
CGCAAGATCG CCGAGCTGCG CCATCGCACG ATCCAGCTCC ACCGGGAGAT CGTCCTGATG 
AGCAAGTACA GCAGCACGGA GATCCAGAAG GAAGACCTCC AGCTGGGCAT CCCCCCCTCC 
TTCATGCGCT TCCAGGCCCG CCAGAGAGAG GAGATCCTGG AGTGGGAGTT CCTTACTGGG 
AAATACTTAT ACTCAGCCAC AGATGGCCAG CCTCCCCGCA GAGGGATGGA CTCAGCACAG 
AGGGAGGCCT TGGATGACAT AGTCATGCAA GTGATGGAGA TGATCAATGC CAACGCCAAG 
ACTCGCGGGC GCATCATCGA CTTCAAGGAG ATCCAGTATG GCTACAGGCG TGTGAACCCC 
ATGTACGGGG CAGAGTACAT TCTGGACCTG CTGCTCCTGT ATAAGAAACA CAAAGGGAAG 
AAGATGACGG TCCCTGTGAG GCGGCACGCG TACCTGCAGC AGACCTTCAG CAAGATGCAG 
TTTGTGGAGC ACGAGGAGCT GGATGCACAA GAGCTGGCAG ACAGAATCAA CCAGGACTCT 
GGGTCCCTGT CATTCCTGTC CAATTCCCTG AAGAAGCTCG TTGCCTTCCA GCTTCCTGGA 
TCCAAGACGG AGCACAAAGA ACCCAAAGAA AAGAAGATCA ACATTCTGAT CCCGTTGTCT 
GGGCGCTTCG ACATGTTTGT GAGGTTCATG GGGAACTTTG AGAAGACGTG TCTCATTCCA 
AATCTGAACG TGAAGCTGGT CATTCTGCTT TTCAACTCTG ACTCTAACCC CGACAAGGCC 
AAACAGGTCG AGCTGATGCG AGATTACCGA GTCAAGTACC CTAAGGCTGA CATGCAGGTC 
CTGCCTGTGT CGGGAGGGTT TTCAAGAGCA CTGGCCCTAG AAGTAGGCTC TTCCCAGTTT 
AACAATGAGT CTTTGCTCTT CTTCTGTGAT GTGGACCTGG TGTTTACAGC GGAATTTTTA 
CAGAGGTGCC GAGCCAACAC AGTGCTGGGG CAGCAGATCT ATTTCCCAAT CATCTTTAGC 
CAGTATGATC CAAAGATTGT TTATAGTGGG AAGGTTCCCA GTGACAACCA TTTTGCCTTT 
ACTCAGAAAA CAGGTTTCTG GAGAAACTAT GGGTTTGGCA TCACTTGTAT TTACAAGGGA 
GATCTTGTCA GAGTGGGTGG CTTCGATGTC TCCATCCAAG GCTGGGGGCT GGAGGATGTG 
GACCTCTTTA ACAAGGTTGT CCAGGCAGGG TTGAAGACAT TCAGGAGCCA GGAGGTTGGA 
GTTGTTCACA TCCACCACCC TGTCGTCTGT GACCCTAATC TGGACCCCAA ACAGTATAAA 
ATGTGCTTGG GGTCGAAAGC ATCGACATTT GGGTCCACGC AGCAGCTGGC CGAAATGTGG 
CTAGAAAAGA ATGACCCCAG TTACAGCAAA GGTGGCAGCC ACGGCTCTGC CAGGACAGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074632.1
CDS	420..2822
Translation	MAARGRRAWLSMLLGLVLGFVLASRLVLPRASELKRVGPRRRPSPEGCRPGQEASQPGGARGDARGAQLWPQGSAAEGVPRDRNFLFVGVMTAQKYLQTRAVAAYRTWSKTIPGKVEFFSSEGSDTSIPIPVVPLRGVDDSYPPQKKSFMMLKYMHDHYLDKYEWFMRADDDVYIKGDRLESFLRSLNSSEPLFLGQTGLGTTEEMGKLALEPGENFCMGGPGVILSREVLRRMAPHIGKCLREMYTTHEDVEVGRCVRRFAGVQCVWSYEMQQLFYENYEQNKKGYIRDLHSSKIHRAITLHPNKNPPYQYRLHSYMLSRKIAELRHRTIQLHREIVLMSKYSSTEIQKEDLQLGIPPSFMRFQARQREEILEWEFLTGKYLYSATDGQPPRRGMDSAQREALDDIVMQVMEMINANAKTRGRIIDFKEIQYGYRRVNPMYGAEYILDLLLLYKKHKGKKMTVPVRRHAYLQQTFSKMQFVEHEELDAQELADRINQDSGSLSFLSNSLKKLVAFQLPGSKTEHKEPKEKKINILIPLSGRFDMFVRFMGNFEKTCLIPNLNVKLVILLFNSDSNPDKAKQVELMRDYRVKYPKADMQVLPVSGGFSRALALEVGSSQFNNESLLFFCDVDLVFTAEFLQRCRANTVLGQQIYFPIIFSQYDPKIVYSGKVPSDNHFAFTQKTGFWRNYGFGITCIYKGDLVRVGGFDVSIQGWGLEDVDLFNKVVQAGLKTFRSQEVGVVHIHHPVVCDPNLDPKQYKMCLGSKASTFGSTQQLAEMWLEKNDPSYSKGGSHGSARTA

Target ORF information:

RefSeq Version	NM_001081163.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus chondroitin sulfate synthase 1 (Chsy1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001081163.2

ORF Insert Sequence:

ATGGCCGCGC GCGGCCGGCG CGCCTGGCTC AGCATGCTGC TCGGGCTCGT GCTGGGCTTC 
GTGCTGGCCT CGCGGCTCGT CCTGCCCCGC GCCTCGGAGC TGAAGCGAGT GGGCCCGCGG 
CGGCGCCCCA GTCCCGAGGG ATGCCGGCCA GGGCAGGAGG CGTCGCAGCC CGGCGGGGCG 
CGCGGCGATG CGCGCGGGGC GCAGCTCTGG CCGCAGGGCT CGGCCGCGGA GGGCGTCCCG 
CGCGACAGGA ACTTTCTCTT CGTGGGAGTC ATGACAGCCC AGAAATACCT GCAGACCCGC 
GCCGTGGCCG CCTACAGAAC ATGGTCCAAG ACAATTCCCG GGAAGGTGGA GTTCTTCTCT 
AGCGAGGGTT CAGATACATC TATACCCATT CCAGTGGTGC CCCTGCGGGG TGTGGATGAC 
TCCTACCCAC CCCAGAAGAA GTCTTTCATG ATGCTCAAGT ACATGCACGA TCACTACCTG 
GACAAGTACG AGTGGTTTAT GAGGGCAGAC GATGACGTTT ACATCAAGGG AGATCGTCTG 
GAGAGTTTCC TGAGGAGTTT GAATAGCAGC GAGCCCCTCT TCCTCGGGCA GACAGGACTG 
GGCACCACAG AAGAAATGGG GAAGCTGGCC CTTGAGCCTG GAGAGAACTT CTGCATGGGC 
GGGCCTGGTG TGATTCTGAG CCGGGAAGTG CTTCGGAGAA TGGCACCACA CATTGGCAAG 
TGTCTCCGGG AAATGTACAC CACCCATGAG GATGTAGAGG TGGGGAGATG TGTCCGGAGA 
TTCGCAGGCG TGCAGTGTGT CTGGTCTTAT GAGATGCAGC AGCTTTTTTA TGAGAATTAT 
GAACAGAACA AGAAAGGGTA CATTAGAGAT CTCCATAGCA GTAAAATCCA CCGAGCGATC 
ACCTTACACC CCAACAAAAA CCCACCGTAC CAGTACAGGC TGCACAGTTA CATGCTCAGC 
CGCAAGATCG CCGAGCTGCG CCATCGCACG ATCCAGCTCC ACCGGGAGAT CGTCCTGATG 
AGCAAGTACA GCAGCACGGA GATCCAGAAG GAAGACCTCC AGCTGGGCAT CCCCCCCTCC 
TTCATGCGCT TCCAGGCCCG CCAGAGAGAG GAGATCCTGG AGTGGGAGTT CCTTACTGGG 
AAATACTTAT ACTCAGCCAC AGATGGCCAG CCTCCCCGCA GAGGGATGGA CTCAGCACAG 
AGGGAGGCCT TGGATGACAT AGTCATGCAA GTGATGGAGA TGATCAATGC CAACGCCAAG 
ACTCGCGGGC GCATCATCGA CTTCAAGGAG ATCCAGTATG GCTACAGGCG TGTGAACCCC 
ATGTACGGGG CAGAGTACAT TCTGGACCTG CTGCTCCTGT ATAAGAAACA CAAAGGGAAG 
AAGATGACGG TCCCTGTGAG GCGGCACGCG TACCTGCAGC AGACCTTCAG CAAGATGCAG 
TTTGTGGAGC ACGAGGAGCT GGATGCACAA GAGCTGGCAG ACAGAATCAA CCAGGACTCT 
GGGTCCCTGT CATTCCTGTC CAATTCCCTG AAGAAGCTCG TTGCCTTCCA GCTTCCTGGA 
TCCAAGACGG AGCACAAAGA ACCCAAAGAA AAGAAGATCA ACATTCTGAT CCCGTTGTCT 
GGGCGCTTCG ACATGTTTGT GAGGTTCATG GGGAACTTTG AGAAGACGTG TCTCATTCCA 
AATCTGAACG TGAAGCTGGT CATTCTGCTT TTCAACTCTG ACTCTAACCC CGACAAGGCC 
AAACAGGTCG AGCTGATGCG AGATTACCGA GTCAAGTACC CTAAGGCTGA CATGCAGGTC 
CTGCCTGTGT CGGGAGGGTT TTCAAGAGCA CTGGCCCTAG AAGTAGGCTC TTCCCAGTTT 
AACAATGAGT CTTTGCTCTT CTTCTGTGAT GTGGACCTGG TGTTTACAGC GGAATTTTTA 
CAGAGGTGCC GAGCCAACAC AGTGCTGGGG CAGCAGATCT ATTTCCCAAT CATCTTTAGC 
CAGTATGATC CAAAGATTGT TTATAGTGGG AAGGTTCCCA GTGACAACCA TTTTGCCTTT 
ACTCAGAAAA CAGGTTTCTG GAGAAACTAT GGGTTTGGCA TCACTTGTAT TTACAAGGGA 
GATCTTGTCA GAGTGGGTGG CTTCGATGTC TCCATCCAAG GCTGGGGGCT GGAGGATGTG 
GACCTCTTTA ACAAGGTTGT CCAGGCAGGG TTGAAGACAT TCAGGAGCCA GGAGGTTGGA 
GTTGTTCACA TCCACCACCC TGTCGTCTGT GACCCTAATC TGGACCCCAA ACAGTATAAA 
ATGTGCTTGG GGTCGAAAGC ATCGACATTT GGGTCCACGC AGCAGCTGGC CGAAATGTGG 
CTAGAAAAGA ATGACCCCAG TTACAGCAAA GGTGGCAGCC ACGGCTCTGC CAGGACAGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

GlcUA?1-3Gal?1-3Gal?1-4Xyl(2-O-phosphate) is the preferred substrate for chondroitin N-acetylgalactosaminyltransferase-1.
The Journal of biological chemistry290(9)5438-48(2015 Feb)
Izumikawa T,Sato B,Mikami T,Tamura J,Igarashi M,Kitagawa H

Chondroitin sulfate synthase 1 (Chsy1) is required for bone development and digit patterning.
Developmental biology363(2)413-25(2012 Mar)
Wilson DG,Phamluong K,Lin WY,Barck K,Carano RA,Diehl L,Peterson AS,Martin F,Solloway MJ

Chondroitin sulfate synthase-2 is necessary for chain extension of chondroitin sulfate but not critical for skeletal development.
PloS one7(8)e43806(2012)
Ogawa H,Hatano S,Sugiura N,Nagai N,Sato T,Shimizu K,Kimata K,Narimatsu H,Watanabe H

A mouse knockout library for secreted and transmembrane proteins.
Nature biotechnology28(7)749-55(2010 Jul)
Tang T,Li L,Tang J,Li Y,Lin WY,Martin F,Grant D,Solloway M,Parker L,Ye W,Forrest W,Ghilardi N,Oravecz T,Platt KA,Rice DS,Hansen GM,Abuin A,Eberhart DE,Godowski P,Holt KH,Peterson A,Zambrowicz BP,de Sauvage FJ

Chondroitin sulfate N-acetylgalactosaminyltransferase-1 plays a critical role in chondroitin sulfate synthesis in cartilage.
The Journal of biological chemistry282(6)4152-61(2007 Feb)
Sakai K,Kimata K,Sato T,Gotoh M,Narimatsu H,Shinomiya K,Watanabe H

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Chsy1 cDNA ORF clone, Mus musculus(house mouse)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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