Nat8l cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nat8l
Entrez Gene ID	269642
Full Name	N-acetyltransferase 8-like
Synonyms	1110038O08Rik,Shati
General protein information	Preferred Names N-acetyltransferase 8-like Names N-acetylaspartate synthetase N-acetyltransferase 8-like protein NAA synthase NAA synthetase aspartate N-acetyltransferase protein Shati
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 B2

mRNA and Protein(s)

mRNA	Protein	Name
NM_001001985.3	NP_001001985.3	N-acetylaspartate synthetase

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	NAT8L	XP_853267.2
Homo sapiens (human)	NAT8L	NP_848652.2
Mus musculus (house mouse)	Nat8l	NP_001001985.3
Xenopus tropicalis (tropical clawed frog)	nat8l	NP_001096350.1
Rattus norvegicus (Norway rat)	Nat8l	NP_001178610.1
Gallus gallus (chicken)	NAT8L	XP_420835.4
Danio rerio (zebrafish)	nat8l	NP_001077308.1

Gene Ontology
Bibliography

Related articles in PubMed

Shati/Nat8l knockout mice show behavioral deficits ameliorated by atomoxetine and methylphenidate.
Toriumi K, Tanaka J, Mamiya T, Alkam T, Kim HC, Nitta A, Nabeshima T
Behavioural brain research339207-214(2018 Feb)

Uncoupling N-acetylaspartate from brain pathology: implications for Canavan disease gene therapy.
von Jonquieres G, Spencer ZHT, Rowlands BD, Klugmann CB, Bongers A, Harasta AE, Parley KE, Cederholm J, Teahan O, Pickford R, Delerue F, Ittner LM, Fr?hlich D, McLean CA, Don AS, Schneider M, Housley GD, Rae CD, Klugmann M
Acta neuropathologica135(1)95-113(2018 Jan)

Striatal N-Acetylaspartate Synthetase Shati/Nat8l Regulates Depression-Like Behaviors via mGluR3-Mediated Serotonergic Suppression in Mice.
Miyamoto Y, Iegaki N, Fu K, Ishikawa Y, Sumi K, Azuma S, Uno K, Muramatsu SI, Nitta A
The international journal of neuropsychopharmacology20(12)1027-1035(2017 Dec)

Suppressing N-Acetyl-l-Aspartate Synthesis Prevents Loss of Neurons in a Murine Model of Canavan Leukodystrophy.
Sohn J, Bannerman P, Guo F, Burns T, Miers L, Croteau C, Singhal NK, McDonough JA, Pleasure D
The Journal of neuroscience : the official journal of the Society for Neuroscience37(2)413-421(2017 Jan)

Methamphetamine induces Shati/Nat8L expression in the mouse nucleus accumbens via CREB- and dopamine D1 receptor-dependent mechanism.
Uno K, Miyazaki T, Sodeyama K, Miyamoto Y, Nitta A
PloS one12(3)e0174196(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results showed that the Shati/Nat8L mRNA was increased by methamphetamine-induced CREB pathway via dopamine D1 receptor signaling in mouse nucleus accumbens. These findings may contribute to development of a clinical tool for methamphetamine addiction.
Title: Methamphetamine induces Shati/Nat8L expression in the mouse nucleus accumbens via CREB- and dopamine D1 receptor-dependent mechanism.

Results showed that both Shati+/- and Shati-/- mice demonstrated lower levels of NAA in the brain than Shati+/+ mice, suggesting that SHATI predominantly regulates NAA content in the brain
Title: Deletion of SHATI/NAT8L decreases the N-acetylaspartate content in the brain and induces behavioral deficits, which can be ameliorated by administering N-acetylaspartate.

Study shows that Shati/Nat8l in the neuron may induce axon outgrowth by ATP synthesis and not through mGluR3 signaling.
Title: Induction of neuronal axon outgrowth by Shati/Nat8l by energy metabolism in mice cultured neurons.

Dysregulated expression of Nat8L is associated with deficits in oxidative phosphorylation in 5xFAD model of Alzheimer disease.
Title: Transcriptional regulation of N-acetylaspartate metabolism in the 5xFAD model of Alzheimer's disease: evidence for neuron-glia communication during energetic crisis.

results indicate that Shati/Nat8l in the Nucleus Accumbens, but not in the dorsal striatum, plays a suppressive role in the behavioral responses to METHamphetamine by controlling the dopaminergic system via activation of group II mGlutamate receptors
Title: Overexpression of Shati/Nat8l, an N-acetyltransferase, in the nucleus accumbens attenuates the response to methamphetamine via activation of group II mGluRs in mice.

The following Nat8l gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nat8l cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu10442	NM_001001985.3 Latest version!	Mus musculus N-acetyltransferase 8-like (Nat8l), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$188.30 $269.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nat8l ( NM_001001985.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001001985.3 Mus musculus N-acetyltransferase 8-like (Nat8l), mRNA.

Price & Availability↑

CloneID	OMu10442
Clone ID Related Accession (Same CDS sequence)	NM_001001985.3
Accession Version	NM_001001985.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 900bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	N-acetylaspartate synthetase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC163329.6 and AK147594.1. On Mar 30, 2007 this sequence version replaced NM_001001985.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK147307.1, AK147703.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCATTGTG GGCCTCCCGA CATGGTCTGC GAGACGAAGA TCGTGGCTAC GGAGGACCAT 
GAGGCACTGC CGGGGGCCAA GAAGGACGCG CTGCTCGTCG CCGCCGGAGC CATGTGGCCC 
CCGCTGCCCG CCGCGCCCGG GCCGGCCGCC GCACCCCCAC CCGCGGCAGG TCCCCAGCCC 
CACGGAGGCA CCGGGGGCGC GGGGCCGCCG GAGGGGCGCG GCGTGTGCAT CCGCGAGTTC 
CGCGCAGCCG AGCAGGAGGC GGCGCGCCGC ATCTTCTACG ACGGCATCTT GGAGCGCATC 
CCCAACACGG CTTTCCGCGG CCTGCGGCAG CACCCGCGCA CCCAACTGCT CTACGCCCTG 
CTGGCCGCCC TCTGTTTTGC TGTGACCCGC TCACTGCTGC TGACATGCCT GGTGCCGGCC 
GGGCTCCTGG CCCTGCGCTA CTACTACAGC CGCAAGGTGA TTCTGGCCTA CCTGGAGTGT 
GCGCTGCACA CGGACATGGC TGACATTGAG CAGTACTACA TGAAGCCACC TGGTTCCTGT 
TTCTGGGTGG CTGTGCTGGA CGGCAACGTT GTGGGCATCG TGGCCGCACG GGCCCATGAG 
GAGGACAACA CAGTGGAGCT GCTCCGCATG TCCGTGGACT CGCGTTTCCG CGGAAAAGGC 
ATCGCCAAGG CTCTGGGCAG GAGGGTGTTG GAGTTTGCCA TGCTGCACAA CTACTCTGCA 
GTGGTACTGG GCACCACAGC CGTTAAGGTG GCCGCCCACA AGCTCTATGA GTCACTGGGC 
TTCAGACACA TGGGCGCGAG TGATCACTAC GTGCTGCCTG GCATGACCCT CTCGCTGGCC 
GAGCGCCTCT TCTTCCAGGT CCGCTACCAC CGCTACCGCC TGCAGCTGCG CGAGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001001985.3
CDS	861..1760
Translation	MHCGPPDMVCETKIVATEDHEALPGAKKDALLVAAGAMWPPLPAAPGPAAAPPPAAGPQPHGGTGGAGPPEGRGVCIREFRAAEQEAARRIFYDGILERIPNTAFRGLRQHPRTQLLYALLAALCFAVTRSLLLTCLVPAGLLALRYYYSRKVILAYLECALHTDMADIEQYYMKPPGSCFWVAVLDGNVVGIVAARAHEEDNTVELLRMSVDSRFRGKGIAKALGRRVLEFAMLHNYSAVVLGTTAVKVAAHKLYESLGFRHMGASDHYVLPGMTLSLAERLFFQVRYHRYRLQLREE

Target ORF information:

RefSeq Version	NM_001001985.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus N-acetyltransferase 8-like (Nat8l), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001001985.3

ORF Insert Sequence:

ATGCATTGTG GGCCTCCCGA CATGGTCTGC GAGACGAAGA TCGTGGCTAC GGAGGACCAT 
GAGGCACTGC CGGGGGCCAA GAAGGACGCG CTGCTCGTCG CCGCCGGAGC CATGTGGCCC 
CCGCTGCCCG CCGCGCCCGG GCCGGCCGCC GCACCCCCAC CCGCGGCAGG TCCCCAGCCC 
CACGGAGGCA CCGGGGGCGC GGGGCCGCCG GAGGGGCGCG GCGTGTGCAT CCGCGAGTTC 
CGCGCAGCCG AGCAGGAGGC GGCGCGCCGC ATCTTCTACG ACGGCATCTT GGAGCGCATC 
CCCAACACGG CTTTCCGCGG CCTGCGGCAG CACCCGCGCA CCCAACTGCT CTACGCCCTG 
CTGGCCGCCC TCTGTTTTGC TGTGACCCGC TCACTGCTGC TGACATGCCT GGTGCCGGCC 
GGGCTCCTGG CCCTGCGCTA CTACTACAGC CGCAAGGTGA TTCTGGCCTA CCTGGAGTGT 
GCGCTGCACA CGGACATGGC TGACATTGAG CAGTACTACA TGAAGCCACC TGGTTCCTGT 
TTCTGGGTGG CTGTGCTGGA CGGCAACGTT GTGGGCATCG TGGCCGCACG GGCCCATGAG 
GAGGACAACA CAGTGGAGCT GCTCCGCATG TCCGTGGACT CGCGTTTCCG CGGAAAAGGC 
ATCGCCAAGG CTCTGGGCAG GAGGGTGTTG GAGTTTGCCA TGCTGCACAA CTACTCTGCA 
GTGGTACTGG GCACCACAGC CGTTAAGGTG GCCGCCCACA AGCTCTATGA GTCACTGGGC 
TTCAGACACA TGGGCGCGAG TGATCACTAC GTGCTGCCTG GCATGACCCT CTCGCTGGCC 
GAGCGCCTCT TCTTCCAGGT CCGCTACCAC CGCTACCGCC TGCAGCTGCG CGAGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Shati/Nat8l knockout mice show behavioral deficits ameliorated by atomoxetine and methylphenidate.
Behavioural brain research339207-214(2018 Feb)
Toriumi K,Tanaka J,Mamiya T,Alkam T,Kim HC,Nitta A,Nabeshima T

Uncoupling N-acetylaspartate from brain pathology: implications for Canavan disease gene therapy.
Acta neuropathologica135(1)95-113(2018 Jan)
von Jonquieres G,Spencer ZHT,Rowlands BD,Klugmann CB,Bongers A,Harasta AE,Parley KE,Cederholm J,Teahan O,Pickford R,Delerue F,Ittner LM,Fr?hlich D,McLean CA,Don AS,Schneider M,Housley GD,Rae CD,Klugmann M

Striatal N-Acetylaspartate Synthetase Shati/Nat8l Regulates Depression-Like Behaviors via mGluR3-Mediated Serotonergic Suppression in Mice.
The international journal of neuropsychopharmacology20(12)1027-1035(2017 Dec)
Miyamoto Y,Iegaki N,Fu K,Ishikawa Y,Sumi K,Azuma S,Uno K,Muramatsu SI,Nitta A

Suppressing N-Acetyl-l-Aspartate Synthesis Prevents Loss of Neurons in a Murine Model of Canavan Leukodystrophy.
The Journal of neuroscience : the official journal of the Society for Neuroscience37(2)413-421(2017 Jan)
Sohn J,Bannerman P,Guo F,Burns T,Miers L,Croteau C,Singhal NK,McDonough JA,Pleasure D

Methamphetamine induces Shati/Nat8L expression in the mouse nucleus accumbens via CREB- and dopamine D1 receptor-dependent mechanism.
PloS one12(3)e0174196(2017)
Uno K,Miyazaki T,Sodeyama K,Miyamoto Y,Nitta A

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Nat8l cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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