Ahcy cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ahcy
Entrez Gene ID	269378
Full Name	S-adenosylhomocysteine hydrolase
Synonyms	AA987153,AL024110,CuBP,SAHH
General protein information	Preferred Names S-adenosylhomocysteine hydrolase Names adenosylhomocysteinase S-adenosyl-L-homocysteine hydrolase adoHcyase liver copper-binding protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2 H1\|2 76.94 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_016661.3	NP_057870.3	adenosylhomocysteinase

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	AHCY	XP_417331.4
Homo sapiens (human)	AHCY	NP_000678.1
Canis lupus familiaris (dog)	AHCY	XP_534388.2
Bos taurus (cattle)	AHCY	NP_001029487.1
Mus musculus (house mouse)	Ahcy	NP_057870.3
	AHCY	XP_001089745.1
Saccharomyces cerevisiae (baker's yeast)	SAH1	NP_010961.3
Kluyveromyces lactis	KLLA0A01199g	XP_451052.1
Eremothecium gossypii	AGOS_AFR243C	NP_985790.1
Pan troglodytes (chimpanzee)	AHCY	XP_514594.2
Rattus norvegicus (Norway rat)	Ahcy	NP_058897.1
Danio rerio (zebrafish)	ahcy	NP_954688.1
Anopheles gambiae (African malaria mosquito)	SAHH_ANOGA	XP_311257.2
Neurospora crassa	NCU07930	XP_962446.1
Arabidopsis thaliana (thale cress)	MEE58	NP_193130.1
Drosophila melanogaster (fruit fly)	Ahcy13	NP_511164.2
Caenorhabditis elegans (roundworm)	ahcy-1	NP_491955.1
Schizosaccharomyces pombe (fission yeast)	SPBC8D2.18c	NP_595580.1
Magnaporthe oryzae (rice blast fungus)	MGG_05155	XP_003712689.1
Arabidopsis thaliana (thale cress)	SAHH2	NP_189023.1
Oryza sativa (rice)	Os11g0455500	NP_001067845.2
Xenopus tropicalis (tropical clawed frog)	ahcy	NP_001037894.1

Gene Ontology
Bibliography

Related articles in PubMed

Epigenetically mediated inhibition of
Pogribny IP, Dreval K, Kindrat I, Melnyk S, Jimenez L, de Conti A, Tryndyak V, Pogribna M, Ortega JF, James SJ, Rusyn I, Beland FA
FASEB journal : official publication of the Federation of American Societies for Experimental Biology(2017 Nov)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

H19 lncRNA alters DNA methylation genome wide by regulating S-adenosylhomocysteine hydrolase.
Zhou J, Yang L, Zhong T, Mueller M, Men Y, Zhang N, Xie J, Giang K, Chung H, Sun X, Lu L, Carmichael GG, Taylor HS, Huang Y
Nature communications610221(2015 Dec)

Coupling global methylation and gene expression profiles reveal key pathophysiological events in liver injury induced by a methyl-deficient diet.
Tryndyak VP, Han T, Muskhelishvili L, Fuscoe JC, Ross SA, Beland FA, Pogribny IP
Molecular nutrition & food research55(3)411-8(2011 Mar)

Crystallization of mouse S-adenosyl-L-homocysteine hydrolase.
Ishihara M, Kusakabe Y, Ohsumichi T, Tanaka N, Nakanishi M, Kitade Y, Nakamura KT
Acta crystallographica. Section F, Structural biology and crystallization communications66(Pt 3)313-5(2010 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

H19 knockdown activates SAHH, leading to increased DNMT3B-mediated methylation of an lncRNA-encoding gene Nctc1 within the Igf2-H19-Nctc1 locus.
Title: H19 lncRNA alters DNA methylation genome wide by regulating S-adenosylhomocysteine hydrolase.

Ahcy was identified from coupling of methylation with gene expression data, shed light on the underlying mechanisms of cytosine demethylation under methyl-deficient conditions.
Title: Coupling global methylation and gene expression profiles reveal key pathophysiological events in liver injury induced by a methyl-deficient diet.

report the crystallization of mouse SAHH in the presence of the reaction product adenosine. The crystals diffracted to at least 1.55 A degrees resolution and are suitable for X-ray structure analysis at high resolution.
Title: Crystallization of mouse S-adenosyl-L-homocysteine hydrolase.

report that Myc promotes upregulation of S-adenosyl homocysteine hydrolase.
Title: S-adenosyl homocysteine hydrolase is required for Myc-induced mRNA cap methylation, protein synthesis, and cell proliferation.

The following Ahcy gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ahcy cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu10370	NM_016661.3 Latest version!	Mus musculus S-adenosylhomocysteine hydrolase (Ahcy), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ahcy ( NM_016661.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_057870.3 Mus musculus S-adenosylhomocysteine hydrolase (Ahcy), mRNA.

Price & Availability↑

CloneID	OMu10370
Clone ID Related Accession (Same CDS sequence)	NM_016661.3
Accession Version	NM_016661.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1299bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Mus musculus(house mouse)
Product	adenosylhomocysteinase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BQ929555.1, AK169331.1, BC086781.1 and AA237720.1. On Oct 31, 2009 this sequence version replaced NM_016661.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC086781.1, BC108367.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849386 [ECO:0000348] ##Evidence-Data-END##

ATGTCTGATA AACTGCCCTA CAAAGTCGCG GACATCGGAC TGGCCGCCTG GGGACGGAAG 
GCTCTGGATA TAGCTGAGAA TGAGATGCCA GGATTGATGC GCATGCGGGA GATGTACTCA 
GCCTCCAAGC CACTGAAGGG TGCTCGCATT GCTGGCTGCC TGCACATGAC CGTGGAGACT 
GCTGTTCTCA TTGAGACTCT CGTGGCCCTG GGTGCTGAGG TGCGGTGGTC CAGCTGCAAC 
ATCTTCTCTA CTCAGGACCA TGCAGCGGCT GCCATTGCCA AGGCTGGCAT TCCAGTGTTT 
GCCTGGAAGG GCGAGACAGA TGAGGAGTAC CTGTGGTGCA TTGAGCAGAC GCTGCACTTC 
AAGGACGGAC CCCTCAACAT GATTCTGGAT GATGGTGGTG ACCTTACTAA CCTCATCCAC 
ACCAAATACC CACAGCTTCT GTCAGGCATC CGAGGTATCT CTGAGGAGAC CACGACTGGG 
GTCCACAACC TCTACAAGAT GATGTCCAAT GGGATACTGA AGGTGCCTGC CATCAATGTC 
AACGATTCTG TCACCAAGAG CAAGTTTGAC AACCTCTATG GCTGCCGGGA GTCCCTCATA 
GATGGCATCA AACGGGCCAC AGATGTGATG ATTGCGGGCA AGGTGGCGGT GGTGGCAGGC 
TATGGTGATG TGGGCAAGGG CTGTGCCCAG GCCCTGAGGG GTTTTGGGGC CCGAGTCATC 
ATCACCGAGA TCGACCCCAT CAATGCACTG CAAGCTGCCA TGGAGGGCTA TGAGGTAACC 
ACTATGGACG AAGCCTGTAA GGAGGGCAAC ATCTTTGTCA CCACCACAGG CTGTGTGGAT 
ATCATCCTTG GCCGGCACTT TGAGCAGATG AAGGATGACG CCATTGTCTG TAACATTGGA 
CACTTCGATG TGGAGATTGA TGTGAAGTGG CTCAATGAGA ACGCGGTGGA GAAAGTGAAC 
ATCAAGCCCC AGGTGGACCG CTACTGGCTA AAGAATGGGC GCCGCATCAT CTTGCTGGCT 
GAAGGCCGTC TGGTCAACCT GGGTTGTGCC ATGGGACACC CCAGCTTCGT GATGAGCAAC 
TCCTTCACAA ACCAGGTGAT GGCACAGATT GAGCTGTGGA CCCACCCAGA TAAATACCCT 
GTTGGGGTTC ACTTCCTGCC TAAGAAGCTG GATGAGGCGG TGGCTGAAGC CCACCTGGGC 
AAGCTGAATG TGAAGCTGAC CAAGCTGACT GAGAAGCAGG CCCAGTACCT GGGCATGCCC 
ATCAACGGCC CCTTCAAGCC TGATCACTAC CGCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057870.3
CDS	106..1404
Translation	MSDKLPYKVADIGLAAWGRKALDIAENEMPGLMRMREMYSASKPLKGARIAGCLHMTVETAVLIETLVALGAEVRWSSCNIFSTQDHAAAAIAKAGIPVFAWKGETDEEYLWCIEQTLHFKDGPLNMILDDGGDLTNLIHTKYPQLLSGIRGISEETTTGVHNLYKMMSNGILKVPAINVNDSVTKSKFDNLYGCRESLIDGIKRATDVMIAGKVAVVAGYGDVGKGCAQALRGFGARVIITEIDPINALQAAMEGYEVTTMDEACKEGNIFVTTTGCVDIILGRHFEQMKDDAIVCNIGHFDVEIDVKWLNENAVEKVNIKPQVDRYWLKNGRRIILLAEGRLVNLGCAMGHPSFVMSNSFTNQVMAQIELWTHPDKYPVGVHFLPKKLDEAVAEAHLGKLNVKLTKLTEKQAQYLGMPINGPFKPDHYRY

Target ORF information:

RefSeq Version	NM_016661.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus S-adenosylhomocysteine hydrolase (Ahcy), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016661.3

ORF Insert Sequence:

ATGTCTGATA AACTGCCCTA CAAAGTCGCG GACATCGGAC TGGCCGCCTG GGGACGGAAG 
GCTCTGGATA TAGCTGAGAA TGAGATGCCA GGATTGATGC GCATGCGGGA GATGTACTCA 
GCCTCCAAGC CACTGAAGGG TGCTCGCATT GCTGGCTGCC TGCACATGAC CGTGGAGACT 
GCTGTTCTCA TTGAGACTCT CGTGGCCCTG GGTGCTGAGG TGCGGTGGTC CAGCTGCAAC 
ATCTTCTCTA CTCAGGACCA TGCAGCGGCT GCCATTGCCA AGGCTGGCAT TCCAGTGTTT 
GCCTGGAAGG GCGAGACAGA TGAGGAGTAC CTGTGGTGCA TTGAGCAGAC GCTGCACTTC 
AAGGACGGAC CCCTCAACAT GATTCTGGAT GATGGTGGTG ACCTTACTAA CCTCATCCAC 
ACCAAATACC CACAGCTTCT GTCAGGCATC CGAGGTATCT CTGAGGAGAC CACGACTGGG 
GTCCACAACC TCTACAAGAT GATGTCCAAT GGGATACTGA AGGTGCCTGC CATCAATGTC 
AACGATTCTG TCACCAAGAG CAAGTTTGAC AACCTCTATG GCTGCCGGGA GTCCCTCATA 
GATGGCATCA AACGGGCCAC AGATGTGATG ATTGCGGGCA AGGTGGCGGT GGTGGCAGGC 
TATGGTGATG TGGGCAAGGG CTGTGCCCAG GCCCTGAGGG GTTTTGGGGC CCGAGTCATC 
ATCACCGAGA TCGACCCCAT CAATGCACTG CAAGCTGCCA TGGAGGGCTA TGAGGTAACC 
ACTATGGACG AAGCCTGTAA GGAGGGCAAC ATCTTTGTCA CCACCACAGG CTGTGTGGAT 
ATCATCCTTG GCCGGCACTT TGAGCAGATG AAGGATGACG CCATTGTCTG TAACATTGGA 
CACTTCGATG TGGAGATTGA TGTGAAGTGG CTCAATGAGA ACGCGGTGGA GAAAGTGAAC 
ATCAAGCCCC AGGTGGACCG CTACTGGCTA AAGAATGGGC GCCGCATCAT CTTGCTGGCT 
GAAGGCCGTC TGGTCAACCT GGGTTGTGCC ATGGGACACC CCAGCTTCGT GATGAGCAAC 
TCCTTCACAA ACCAGGTGAT GGCACAGATT GAGCTGTGGA CCCACCCAGA TAAATACCCT 
GTTGGGGTTC ACTTCCTGCC TAAGAAGCTG GATGAGGCGG TGGCTGAAGC CCACCTGGGC 
AAGCTGAATG TGAAGCTGAC CAAGCTGACT GAGAAGCAGG CCCAGTACCT GGGCATGCCC 
ATCAACGGCC CCTTCAAGCC TGATCACTAC CGCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Epigenetically mediated inhibition of
FASEB journal : official publication of the Federation of American Societies for Experimental Biology(2017 Nov)
Pogribny IP,Dreval K,Kindrat I,Melnyk S,Jimenez L,de Conti A,Tryndyak V,Pogribna M,Ortega JF,James SJ,Rusyn I,Beland FA

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

H19 lncRNA alters DNA methylation genome wide by regulating S-adenosylhomocysteine hydrolase.
Nature communications610221(2015 Dec)
Zhou J,Yang L,Zhong T,Mueller M,Men Y,Zhang N,Xie J,Giang K,Chung H,Sun X,Lu L,Carmichael GG,Taylor HS,Huang Y

Coupling global methylation and gene expression profiles reveal key pathophysiological events in liver injury induced by a methyl-deficient diet.
Molecular nutrition & food research55(3)411-8(2011 Mar)
Tryndyak VP,Han T,Muskhelishvili L,Fuscoe JC,Ross SA,Beland FA,Pogribny IP

Crystallization of mouse S-adenosyl-L-homocysteine hydrolase.
Acta crystallographica. Section F, Structural biology and crystallization communications66(Pt 3)313-5(2010 Mar)
Ishihara M,Kusakabe Y,Ohsumichi T,Tanaka N,Nakanishi M,Kitade Y,Nakamura KT

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Ahcy cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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