Sh2d1b1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sh2d1b1
Entrez Gene ID	26904
Full Name	SH2 domain containing 1B1
Synonyms	EAT-2,EAT-2A,Eat2,Eat2a,Sh2d1b
General protein information	Preferred Names SH2 domain containing 1B1 Names SH2 domain-containing protein 1B EWS/FLI1 activated transcript 2 SH2 domain protein 1B1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1\|1 H3

mRNA and Protein(s)

mRNA	Protein	Name
NM_012009.5	NP_036139.3	SH2 domain-containing protein 1B
NM_012009.4	NP_036139.3	SH2 domain-containing protein 1B

Pathways from BioSystems

Homologies

Homo sapiens (human)	SH2D1B	NP_444512.2
Pan troglodytes (chimpanzee)	SH2D1B	XP_003949678.1
Rattus norvegicus (Norway rat)	Sh2d1b	XP_577285.1
Canis lupus familiaris (dog)	SH2D1B	XP_005640915.1
Bos taurus (cattle)	SH2D1B	NP_001180091.1
Mus musculus (house mouse)	Sh2d1b1	NP_036139.3
Danio rerio (zebrafish)	si:dkey-11p10.10	NP_001189421.1
Xenopus tropicalis (tropical clawed frog)	LOC100486929	XP_002932331.1
	SH2D1B	XP_001118174.1
Gallus gallus (chicken)	SH2D1B	NP_001265002.1

Gene Ontology
Bibliography

Related articles in PubMed

A hematopoietic cell-driven mechanism involving SLAMF6 receptor, SAP adaptors and SHP-1 phosphatase regulates NK cell education.
Wu N, Zhong MC, Roncagalli R, P?rez-Quintero LA, Guo H, Zhang Z, Lenoir C, Dong Z, Latour S, Veillette A
Nature immunology17(4)387-96(2016 Apr)

Identification of the SLAM Adapter Molecule EAT-2 as a Lupus-Susceptibility Gene That Acts through Impaired Negative Regulation of Dendritic Cell Signaling.
Talaei N, Yu T, Manion K, Bremner R, Wither JE
Journal of immunology (Baltimore, Md. : 1950)195(10)4623-31(2015 Nov)

Immune cell inhibition by SLAMF7 is mediated by a mechanism requiring src kinases, CD45, and SHIP-1 that is defective in multiple myeloma cells.
Guo H, Cruz-Munoz ME, Wu N, Robbins M, Veillette A
Molecular and cellular biology35(1)41-51(2015 Jan)

EAT-2, a SAP-like adaptor, controls NK cell activation through phospholipase C?, Ca++, and Erk, leading to granule polarization.
P?rez-Quintero LA, Roncagalli R, Guo H, Latour S, Davidson D, Veillette A
The Journal of experimental medicine211(4)727-42(2014 Apr)

Signaling lymphocyte activation molecule regulates development of colitis in mice.
van Driel B, Liao G, Romero X, O'Keeffe MS, Wang G, Faubion WA, Berger SB, Magelky EM, Manocha M, Azcutia V, Grisham M, Luscinskas FW, Mizoguchi E, de Waal Malefyt R, Reinecker HC, Bhan AK, Wang N, Terhorst C
Gastroenterology143(6)1544-1554.e7(2012 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

EAT-2 negatively regulates cytokine production in dendritic cells downstream of SLAM engagement and a genetic polymorphism that disturbs this process promotes the development of lupus.
Title: Identification of the SLAM Adapter Molecule EAT-2 as a Lupus-Susceptibility Gene That Acts through Impaired Negative Regulation of Dendritic Cell Signaling.

EAT-2 mediates its effects in natural killer cells by linking SLAM family receptors to phospholipase Cgamma, calcium fluxes, and Erk kinase.
Title: EAT-2, a SAP-like adaptor, controls NK cell activation through phospholipase C?, Ca++, and Erk, leading to granule polarization.

EAT-2A and EAT-2B act as positive regulators of signaling lymphocyte activation molecule family receptor-specific NK cell functions in C57BL/6 mice.
Title: Cutting edge: The adapters EAT-2A and -2B are positive regulators of CD244- and CD84-dependent NK cell functions in the C57BL/6 mouse.

EAT-2 mediates CRACC function. In the absence of EAT-2, CRACC inhibited natural killer cell function.
Title: Influence of CRACC, a SLAM family receptor coupled to the adaptor EAT-2, on natural killer cell function.

Taken together, the data suggest that both EAT-2A and EAT-2B are adapters that recruit Src kinases to SLAM family receptors using a mechanism that is distinct from that of SAP.
Title: Identification and characterization of two related murine genes, Eat2a and Eat2b, encoding single SH2-domain adapters.

The following Sh2d1b1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sh2d1b1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu09327	NM_012009.5 Latest version!	Mus musculus SH2 domain containing 1B1 (Sh2d1b1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
View Old Accession Versions >>
OMu09327	NM_012009.4	Mus musculus SH2 domain containing 1B1 (Sh2d1b1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sh2d1b1 ( NM_012009.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_036139.3 Mus musculus SH2 domain containing 1B1 (Sh2d1b1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu09327
Clone ID Related Accession (Same CDS sequence)	NM_012009.5 , NM_012009.4
Accession Version	NM_012009.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 399bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Mus musculus(house mouse)
Product	SH2 domain-containing protein 1B
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC123650.7 and BC116698.1. On Feb 16, 2018 this sequence version replaced NM_012009.4. Transcript Variant: This variant (1) represents the longer, protein-coding transcript. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK089191.1, BC116698.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849377 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATCTGC CTTACTACCA TGGCTGCCTG ACCAAGCGAG AGTGTGAAGC CCTGCTCCTC 
AAGGGAGGTG TGGATGGCAA CTTTCTGATA AGAGACAGCG AGTCTGTGCC AGGAGCCCTG 
TGCCTCTGTG TCTCGTTTAA AAAGCTTGTC TACAGCTACC GAATCTTCAG AGAGAAACAT 
GGATATTACA GGATAGAGAC TAATGCTCAT ACTCCAAGAA CGATCTTTCC AAACCTACAG 
GAATTGGTCT CCAAATATGG AAAACCGGGT CAAGGATTGG TGGTTCACCT TTCAAACCCA 
ATAATGAGAA ACAACCTATG CCAAAGAGGG AGAAGAATGG AGTTAGAGCT GAATGTTTAT 
GAGAACACTG ATAAGGAGTA TGTGGACGTC TTGCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036139.3
CDS	59..457
Translation	MDLPYYHGCLTKRECEALLLKGGVDGNFLIRDSESVPGALCLCVSFKKLVYSYRIFREKHGYYRIETNAHTPRTIFPNLQELVSKYGKPGQGLVVHLSNPIMRNNLCQRGRRMELELNVYENTDKEYVDVLP

Target ORF information:

RefSeq Version	NM_012009.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus SH2 domain containing 1B1 (Sh2d1b1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012009.5

ORF Insert Sequence:

ATGGATCTGC CTTACTACCA TGGCTGCCTG ACCAAGCGAG AGTGTGAAGC CCTGCTCCTC 
AAGGGAGGTG TGGATGGCAA CTTTCTGATA AGAGACAGCG AGTCTGTGCC AGGAGCCCTG 
TGCCTCTGTG TCTCGTTTAA AAAGCTTGTC TACAGCTACC GAATCTTCAG AGAGAAACAT 
GGATATTACA GGATAGAGAC TAATGCTCAT ACTCCAAGAA CGATCTTTCC AAACCTACAG 
GAATTGGTCT CCAAATATGG AAAACCGGGT CAAGGATTGG TGGTTCACCT TTCAAACCCA 
ATAATGAGAA ACAACCTATG CCAAAGAGGG AGAAGAATGG AGTTAGAGCT GAATGTTTAT 
GAGAACACTG ATAAGGAGTA TGTGGACGTC TTGCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sh2d1b1 ( NM_012009.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_036139.3 Mus musculus SH2 domain containing 1B1 (Sh2d1b1), mRNA.

Price & Availability↑

CloneID	OMu09327
Clone ID Related Accession (Same CDS sequence)	NM_012009.5 , NM_012009.4
Accession Version	NM_012009.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 399bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Mus musculus(house mouse)
Product	SH2 domain-containing protein 1B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC117508.1. On Jun 10, 2007 this sequence version replaced NM_012009.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC116698.1, AK089191.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849377 [ECO:0000348] ##Evidence-Data-END##

ATGGATCTGC CTTACTACCA TGGCTGCCTG ACCAAGCGAG AGTGTGAAGC CCTGCTCCTC 
AAGGGAGGTG TGGATGGCAA CTTTCTGATA AGAGACAGCG AGTCTGTGCC AGGAGCCCTG 
TGCCTCTGTG TCTCGTTTAA AAAGCTTGTC TACAGCTACC GAATCTTCAG AGAGAAACAT 
GGATATTACA GGATAGAGAC TAATGCTCAT ACTCCAAGAA CGATCTTTCC AAACCTACAG 
GAATTGGTCT CCAAATATGG AAAACCGGGT CAAGGATTGG TGGTTCACCT TTCAAACCCA 
ATAATGAGAA ACAACCTATG CCAAAGAGGG AGAAGAATGG AGTTAGAGCT GAATGTTTAT 
GAGAACACTG ATAAGGAGTA TGTGGACGTC TTGCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036139.3
CDS	53..451
Translation	MDLPYYHGCLTKRECEALLLKGGVDGNFLIRDSESVPGALCLCVSFKKLVYSYRIFREKHGYYRIETNAHTPRTIFPNLQELVSKYGKPGQGLVVHLSNPIMRNNLCQRGRRMELELNVYENTDKEYVDVLP

Target ORF information:

RefSeq Version	NM_012009.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus SH2 domain containing 1B1 (Sh2d1b1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012009.4

ORF Insert Sequence:

ATGGATCTGC CTTACTACCA TGGCTGCCTG ACCAAGCGAG AGTGTGAAGC CCTGCTCCTC 
AAGGGAGGTG TGGATGGCAA CTTTCTGATA AGAGACAGCG AGTCTGTGCC AGGAGCCCTG 
TGCCTCTGTG TCTCGTTTAA AAAGCTTGTC TACAGCTACC GAATCTTCAG AGAGAAACAT 
GGATATTACA GGATAGAGAC TAATGCTCAT ACTCCAAGAA CGATCTTTCC AAACCTACAG 
GAATTGGTCT CCAAATATGG AAAACCGGGT CAAGGATTGG TGGTTCACCT TTCAAACCCA 
ATAATGAGAA ACAACCTATG CCAAAGAGGG AGAAGAATGG AGTTAGAGCT GAATGTTTAT 
GAGAACACTG ATAAGGAGTA TGTGGACGTC TTGCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A hematopoietic cell-driven mechanism involving SLAMF6 receptor, SAP adaptors and SHP-1 phosphatase regulates NK cell education.
Nature immunology17(4)387-96(2016 Apr)
Wu N,Zhong MC,Roncagalli R,P?rez-Quintero LA,Guo H,Zhang Z,Lenoir C,Dong Z,Latour S,Veillette A

Identification of the SLAM Adapter Molecule EAT-2 as a Lupus-Susceptibility Gene That Acts through Impaired Negative Regulation of Dendritic Cell Signaling.
Journal of immunology (Baltimore, Md. : 1950)195(10)4623-31(2015 Nov)
Talaei N,Yu T,Manion K,Bremner R,Wither JE

Immune cell inhibition by SLAMF7 is mediated by a mechanism requiring src kinases, CD45, and SHIP-1 that is defective in multiple myeloma cells.
Molecular and cellular biology35(1)41-51(2015 Jan)
Guo H,Cruz-Munoz ME,Wu N,Robbins M,Veillette A

EAT-2, a SAP-like adaptor, controls NK cell activation through phospholipase C?, Ca++, and Erk, leading to granule polarization.
The Journal of experimental medicine211(4)727-42(2014 Apr)
P?rez-Quintero LA,Roncagalli R,Guo H,Latour S,Davidson D,Veillette A

Signaling lymphocyte activation molecule regulates development of colitis in mice.
Gastroenterology143(6)1544-1554.e7(2012 Dec)
van Driel B,Liao G,Romero X,O'Keeffe MS,Wang G,Faubion WA,Berger SB,Magelky EM,Manocha M,Azcutia V,Grisham M,Luscinskas FW,Mizoguchi E,de Waal Malefyt R,Reinecker HC,Bhan AK,Wang N,Terhorst C

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Sh2d1b1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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