Robo2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Robo2
Entrez Gene ID	268902
Full Name	roundabout guidance receptor 2
Synonyms	2600013A04Rik,9430089E08Rik,BB097918,D230004I22Rik,mKIAA1568
General protein information	Preferred Names roundabout guidance receptor 2 Names roundabout homolog 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 16 Map Location: 16\|16 C3.1

mRNA and Protein(s)

mRNA	Protein	Name
NM_175549.4	NP_780758.3	roundabout homolog 2 precursor
XM_017317013.1	XP_017172502.1	roundabout homolog 2 isoform X8
XM_006523036.3	XP_006523099.1	roundabout homolog 2 isoform X2
XM_006523038.3	XP_006523101.1	roundabout homolog 2 isoform X4
XM_006523041.3	XP_006523104.1	roundabout homolog 2 isoform X7
XM_006523046.3	XP_006523109.1	roundabout homolog 2 isoform X13
XM_017317015.1	XP_017172504.1	roundabout homolog 2 isoform X16
XM_006523035.4	XP_006523098.1	roundabout homolog 2 isoform X1
XM_006523037.4	XP_006523100.1	roundabout homolog 2 isoform X3
XM_006523038.4	XP_006523101.1	roundabout homolog 2 isoform X4
XM_006523040.4	XP_006523103.1	roundabout homolog 2 isoform X6
XM_030249141.1	XP_030105001.1	roundabout homolog 2 isoform X11
XM_017317016.2	XP_017172505.1	roundabout homolog 2 isoform X16
XM_006523037.3	XP_006523100.1	roundabout homolog 2 isoform X3
XM_006523040.3	XP_006523103.1	roundabout homolog 2 isoform X6
XM_011246131.2	XP_011244433.1	roundabout homolog 2 isoform X15
XM_006523049.3	XP_006523112.1	roundabout homolog 2 isoform X20
XM_006523039.4	XP_006523102.1	roundabout homolog 2 isoform X5
XM_006523045.4	XP_006523108.1	roundabout homolog 2 isoform X10
XM_006523046.4	XP_006523109.1	roundabout homolog 2 isoform X12
XM_011246131.3	XP_011244433.1	roundabout homolog 2 isoform X13
NM_001358493.1	NP_001345422.1	roundabout homolog 2 isoform 1
XM_006523035.3	XP_006523098.1	roundabout homolog 2 isoform X1
XM_006523043.3	XP_006523106.1	roundabout homolog 2 isoform X10
XM_017317017.1	XP_017172506.1	roundabout homolog 2 isoform X21
XM_006523044.3	XP_006523107.1	roundabout homolog 2 isoform X11
XM_030249140.1	XP_030105000.1	roundabout homolog 2 isoform X4
XM_006523041.4	XP_006523104.1	roundabout homolog 2 isoform X7
XM_006523043.4	XP_006523106.1	roundabout homolog 2 isoform X9
XM_017317015.2	XP_017172504.1	roundabout homolog 2 isoform X14
XM_006523048.4	XP_006523111.1	roundabout homolog 2 isoform X15
NM_001358492.1	NP_001345421.1	roundabout homolog 2 isoform 2
XM_011246132.2	XP_011244434.1	roundabout homolog 2 isoform X18
XM_006523039.3	XP_006523102.1	roundabout homolog 2 isoform X5
XM_006523048.3	XP_006523111.1	roundabout homolog 2 isoform X17
XM_017317016.1	XP_017172505.1	roundabout homolog 2 isoform X19
XM_006523049.4	XP_006523112.1	roundabout homolog 2 isoform X17
NM_001358491.1	NP_001345420.1	roundabout homolog 2 isoform 3
NM_175549.5	NP_780758.3	roundabout homolog 2 isoform 5 precursor
NM_001358494.1	NP_001345423.1	roundabout homolog 2 isoform 6 precursor
XM_006523042.3	XP_006523105.1	roundabout homolog 2 isoform X9
XM_006523045.3	XP_006523108.1	roundabout homolog 2 isoform X12
XM_006523047.3	XP_006523110.1	roundabout homolog 2 isoform X14
XM_017317018.1	XP_017172507.1	roundabout homolog 2 isoform X22
XM_006523036.4	XP_006523099.1	roundabout homolog 2 isoform X2
XM_030249139.1	XP_030104999.1	roundabout homolog 2 isoform X4
XM_006523042.4	XP_006523105.1	roundabout homolog 2 isoform X8
NM_001358490.1	NP_001345419.1	roundabout homolog 2 isoform 4 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	ROBO2	NP_002933.1
Rattus norvegicus (Norway rat)	Robo2	NP_115289.1
Canis lupus familiaris (dog)	LOC487691	XP_005638813.1
Bos taurus (cattle)	LOC534842	XP_002684698.3
Gallus gallus (chicken)	ROBO2	XP_416674.4
Danio rerio (zebrafish)	robo2	NP_571708.1
	LOC719197	XP_002802664.1
Mus musculus (house mouse)	Robo2	NP_780758.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010969	XP_309726.4
Drosophila melanogaster (fruit fly)	robo	NP_476899.1
Xenopus tropicalis (tropical clawed frog)	robo2	XP_004912137.1
Pan troglodytes (chimpanzee)	ROBO2	XP_516591.3

Gene Ontology

The following Robo2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Robo2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu79345	NM_001358494.1 Latest version!	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu52766	XM_006523046.4 Latest version!	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu52768	XM_006523048.4 Latest version!	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu52769	XM_006523049.4 Latest version!	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu74355	XM_011246131.3 Latest version!	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu79342	XM_017317015.2 Latest version!	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu79343	XM_017317016.2 Latest version!	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu79344	XM_017317017.1 Latest version!	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu79345	XM_017317018.1 Latest version!	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu79341	NM_001358493.1 Latest version!	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu52755	XM_006523035.4 Latest version!	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu52756	XM_006523036.4 Latest version!	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu52757	XM_006523037.4 Latest version!	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu52758	XM_030249140.1 Latest version!	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu52758	XM_006523038.4 Latest version!	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu52758	XM_030249139.1 Latest version!	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu52759	XM_006523039.4 Latest version!	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu52760	XM_006523040.4 Latest version!	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu52761	XM_006523041.4 Latest version!	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu52762	XM_006523042.4 Latest version!	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu52763	XM_006523043.4 Latest version!	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu52765	XM_006523045.4 Latest version!	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu79341	XM_017317013.1 Latest version!	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu86964	XM_030249141.1 Latest version!	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu15146	NM_175549.5 Latest version!	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$888.30 $1269.00
OMu52764	NM_001358490.1 Latest version!	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu52767	NM_001358491.1 Latest version!	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu74356	NM_001358492.1 Latest version!	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu52764	XM_006523044.3 Latest version!	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu52767	XM_006523047.3 Latest version!	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu74356	XM_011246132.2 Latest version!	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
View Old Accession Versions >>
OMu52758	XM_006523038.3	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu52757	XM_006523037.3	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu52760	XM_006523040.3	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu52766	XM_006523046.3	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu74355	XM_011246131.2	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu52755	XM_006523035.3	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu52761	XM_006523041.3	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu52763	XM_006523043.3	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu79342	XM_017317015.1	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu52759	XM_006523039.3	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu52768	XM_006523048.3	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu79343	XM_017317016.1	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu52769	XM_006523049.3	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu15146	NM_175549.4	Mus musculus roundabout guidance receptor 2 (Robo2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$888.30 $1269.00
OMu52765	XM_006523045.3	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu52756	XM_006523036.3	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu52762	XM_006523042.3	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Robo2 ( NM_001358494.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001345423.1 Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant 6, mRNA.

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CloneID	OMu79345
Clone ID Related Accession (Same CDS sequence)	NM_001358494.1 , XM_017317018.1
Accession Version	NM_001358494.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4140bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	roundabout homolog 2 isoform 6 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC158234.2, AC112155.3, AC134452.4, AC122049.2 and AC162374.4. On Dec 1, 2017 this sequence version replaced XM_017317018.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660813.207525.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END##

ATGAATCCTC TGATGTTTAC ACTATTATTG CTCTTTGGAT TTCTCTGCAT TCAGATTGAT 
GGATCTCGTC TTCGTCAAGA AGACTTTCCC CCCAGGATCG TAGAACACCC TTCTGATGTC 
ATCGTCTCCA AGGGAGAGCC CACCACTCTG AACTGTAAAG CAGAGGGCCG ACCCACCCCC 
ACCATTGAAT GGTACAAGGA TGGTGAGAGG GTGGAGACAG ACAAGGATGA TCCCAGGTCC 
CACAGAATGC TTCTGCCCAG CGGATCTTTA TTCTTTTTGC GAATTGTTCA TGGGCGCAGA 
AGTAAACCGG ACGAAGGGAG TTACGTTTGT GTTGCAAGGA ACTATCTTGG TGAAGCAGTG 
AGTCGAAATG CATCTCTGGA AGTGGCATTA TTGCGAGATG ACTTCCGGCA AAACCCCACA 
GATGTGGTAG TCGCAGCTGG AGAGCCTGCA ATCTTGGAGT GCCAGCCACC ACGGGGACAC 
CCAGAACCAA CCATCTACTG GAAAAAGGAC AAAGTCCGAA TTGATGACAA GGAAGAGAGA 
ATAAGTATCC GTGGTGGGAA GCTGATGATC TCTAATACTA GGAAAAGCGA TGCTGGCATG 
TACACCTGTG TGGGAACCAA TATGGTGGGA GAAAGGGACA GCGACCCTGC AGAGCTCACT 
GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTGCT AGAGGAAGAA 
GCTGTAGAAT TCCGTTGTCA GGTCCAAGGA GATCCCCAGC CAACGGTGAG GTGGAAAAAA 
GATGATGCAG ACTTGCCGAG AGGAAGGTAT GATATCAAAG ATGACTACAC GCTGAGAATT 
AAAAAGGCCA TGAGTACTGA TGAAGGTACC TATGTGTGTA TTGCTGAGAA TCGGGTGGGA 
AAAGTGGAAG CCTCTGCTAC CCTCACTGTC CGAGTTCGCC CTGTTGCTCC TCCACAGTTT 
GTGGTTAGGC CAAGAGATCA GATCGTTGCT CAAGGCCGAA CAGTGACATT CCCCTGTGAA 
ACTAAAGGAA ACCCACAGCC AGCTGTTTTT TGGCAGAAAG AAGGCAGCCA GAACCTACTT 
TTCCCGAATC AACCTCAGCA GCCCAACAGC CGATGTTCAG TGTCGCCCAC GGGGGACCTC 
ACCATCACCA ACATCCAGCG TTCAGATGCG GGTTACTACA TCTGCCAGGC CCTAACCGTG 
GCAGGAAGCA TTTTAGCTAA AGCACAGTTG GAAGTTACTG ACGTTTTGAC AGATAGACCT 
CCACCCATAA TCTTGCAAGG ACCAATAAAC CAAACACTTG CAGTAGACGG TACAGCATTG 
TTGAAGTGTA AAGCCACTGG TGAGCCTCTG CCTGTAATTA GCTGGCTAAA GGAGGGCTTT 
ACTTTTCTGG GGAGAGATCC AAGAGCCACG ATCCAAGACC AAGGAACACT GCAGATTAAG 
AATTTACGGA TATCTGATAC TGGCACTTAT ACTTGTGTGG CTACAAGTTC CAGTGGAGAG 
ACTTCCTGGA GTGCAGTGCT GGATGTAACA GAATCTGGAG CAACAATCAG TAAAAATTAT 
GATATGAATG ACCTCCCGGG ACCACCATCC AAACCTCAGG TCACTGATGT TTCTAAGAAC 
AGTGTCACCT TATCCTGGCA GCCAGGTACA CCTGGCGTTC TTCCTGCAAG CGCGTATATC 
ATTGAGGCTT TCAGCCAATC GGTGAGCAAT AGCTGGCAGA CAGTGGCAAA CCATGTTAAG 
ACAACTCTGT ATACAGTAAG GGGGCTGAGG CCCAACACAA TCTACTTGTT TATGGTCAGA 
GCGATCAACC CACAAGGTCT CAGTGATCCA AGTCCTATGT CGGATCCTGT ACGCACACAA 
GATATCAGCC CCCCAGCACA AGGAGTGGAC CACAGACAGG TGCAGAAGGA ATTAGGTGAT 
GTCGTTGTTC GTCTCCATAA TCCAGTTGTC CTGACACCTA CAACTGTTCA AGTCACATGG 
ACGGTGGACC GACAACCCCA GTTTATTCAG GGCTACAGAG TGATGTACCG TCAGACTTCG 
GGACTACAAG CCTCAACTGT GTGGCAGAAT CTAGACGCCA AAGTCCCGAC TGAGAGGAGT 
GCTGTCCTTG TGAATTTGAA AAAGGGGGTG ACTTATGAAA TTAAAGTCCG GCCGTATTTT 
AACGAGTTCC AAGGAATGGA CAGTGAATCG AAAACAGTCC GAACCACTGA GGAAGCCCCA 
AGTGCCCCTC CCCAGTCTGT CACTGTGCTG ACAGTTGGAA GTCACAACAG CACAAGCATC 
AGTGTTTCCT GGGATCCTCC ACCAGCCGAC CACCAGAATG GAATTATTCA GGAATATAAG 
ATCTGGTGTC TGGGAAACGA AACGCGATTC CATATCAATA AAACGGTGGA TGCAGCCATT 
CGCTCTGTAG TAATAGGTGG CTTGTTCCCT GGAATTCAGT ACCGGGTAGA AGTGGCAGCT 
AGCACAAGTG CAGGGGTTGG AGTAAAAAGT GAACCACAGC CGATAATAAT TGGGGGACGT 
AACGAAGTTG TCATTACTGA AAACAATAAC AGCATCACTG AGCAAATCAC GGATGTCGTG 
AAGCAACCGG CATTTATAGC TGGCATTGGT GGTGCCTGCT GGGTAATTCT GATGGGTTTT 
AGCATCTGGT TGTACTGGAG AAGAAAGAAG AGAAAGGGAC TCAGCAATTA TGCTGTAACA 
TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGGAGCC GTCCAGGTCT TCTAAATGCT 
GGCGATCCCA ATTACCCATG GCTTGCTGAT TCTTGGCCAG CCACGAGTTT GCCAGTGAAC 
AATAGCAATA GTGGCCCAAA TGAAATTGGA AATTTTGGGC GTGGAGATGT GCTGCCTCCG 
GTGCCAGGCC AAGGGGATAA AACAGCGACC ATGCTCTCGG ATGGAGCCAT TTATAGCAGC 
ATTGACTTCA CTACCAAAAC CACTTACAAC AGTTCCAGCC AAATAACACA GGCCACCCCA 
TATGCCACTA CACAAATCCT GCATTCAAAC AGCATCCACG AACTGGCAGT TGATCTTCCT 
GATCCACAGT GGAAAAGCTC AGTTCAACAG AAGACAGACC TCATGGGATT TGGTTATTCG 
CTACCTGATC AGAACAAGGG GAACAACGGT GGGAAAGGTG GAAAAAAGAA GAAAACTAAA 
AATTCTTCGA AAGCGCAGAA AAACAACGGA TCCACTTGGG CTAATGTCCC TCTACCTCCT 
CCTCCCGTCC AGCCCCTTCC TGGTACAGAG CTGGGCCACT ATGCTGCGGA ACAAGAAAAT 
GGCTATGACA GTGACAGCTG GTGCCCACCA TTACCGGTGC AAACATACTT GCATCAGGGT 
ATGGAAGATG AGCTGGAAGA AGACGAAGAT CGGGTCCCAA CACCTCCTGT TCGCGGCGTG 
GCCTCTTCAC CAGCTATCTC TTTTGGACAG CAGTCCACTG CCACTCTTAC TCCATCCCCA 
CGGGAAGAGA TGCAACCCAT GCTGCAAGCT CACTTGGATG AGTTGACAAG GGCCTATCAG 
TTTGATATAG CAAAACAAAC ATGGCACATT CAAAGCAATA CCCCACCTCC ACAACCCCCA 
GCTCCGCCAT TAGGTTACGT GTCCGGAGCC TTGATTTCTG ATTTGGAGAC AGATGTTCCA 
GATGAGGATG CTGATGATGA AGAGGAACCA TTAGAAATTC CCAGGCCCCT CAGAGCACTA 
GACCAGACAC CTGGATCCAG TATGGACAAT CTAGACAGCT CTGTCACAGG AAAAGCCTTT 
AGCTCCTCTC AAAGGCAGCG GCCCACCAGC CCATTTTCTA CGGACAGTAA CACCAGTGCT 
GCCCAGAATC AAAGCCAGAG GCCTCGGCCC ACGAAAAAAC ATAAGGGAGG ACGGATGGAC 
CCACAGCCAG TGTTACCTCA TCGGAGGGAA GGGATGCCAG ATGAGGAGTC CCTGGTGCCC 
TACAGCAAGC CCAGCTTCCC GTCTCCTGGC GGGCACAGCT CATCAGGAAC ATCTTCCTCT 
AAAGGGTCCA CTGGCCCTCG GAAGGCTGAC GTGCTGAGAG GCAGCCACCA GAGGAATGCC 
AATGACCTTC TCGACATTGG ATATGTGGGC TCAAATAGTC AAGGACAGTT TACAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001345423.1
CDS	914..5053
Translation	MNPLMFTLLLLFGFLCIQIDGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKAMSTDEGTYVCIAENRVGKVEASATLTVRVRPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPINQTLAVDGTALLKCKATGEPLPVISWLKEGFTFLGRDPRATIQDQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDMNDLPGPPSKPQVTDVSKNSVTLSWQPGTPGVLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVVVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQASTVWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSHNSTSISVSWDPPPADHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVVIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGGRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPNYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTTYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSVQQKTDLMGFGYSLPDQNKGNNGGKGGKKKKTKNSSKAQKNNGSTWANVPLPPPPVQPLPGTELGHYAAEQENGYDSDSWCPPLPVQTYLHQGMEDELEEDEDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNTPPPQPPAPPLGYVSGALISDLETDVPDEDADDEEEPLEIPRPLRALDQTPGSSMDNLDSSVTGKAFSSSQRQRPTSPFSTDSNTSAAQNQSQRPRPTKKHKGGRMDPQPVLPHRREGMPDEESLVPYSKPSFPSPGGHSSSGTSSSKGSTGPRKADVLRGSHQRNANDLLDIGYVGSNSQGQFTE

Target ORF information:

RefSeq Version	NM_001358494.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001358494.1

ORF Insert Sequence:

ATGAATCCTC TGATGTTTAC ACTATTATTG CTCTTTGGAT TTCTCTGCAT TCAGATTGAT 
GGATCTCGTC TTCGTCAAGA AGACTTTCCC CCCAGGATCG TAGAACACCC TTCTGATGTC 
ATCGTCTCCA AGGGAGAGCC CACCACTCTG AACTGTAAAG CAGAGGGCCG ACCCACCCCC 
ACCATTGAAT GGTACAAGGA TGGTGAGAGG GTGGAGACAG ACAAGGATGA TCCCAGGTCC 
CACAGAATGC TTCTGCCCAG CGGATCTTTA TTCTTTTTGC GAATTGTTCA TGGGCGCAGA 
AGTAAACCGG ACGAAGGGAG TTACGTTTGT GTTGCAAGGA ACTATCTTGG TGAAGCAGTG 
AGTCGAAATG CATCTCTGGA AGTGGCATTA TTGCGAGATG ACTTCCGGCA AAACCCCACA 
GATGTGGTAG TCGCAGCTGG AGAGCCTGCA ATCTTGGAGT GCCAGCCACC ACGGGGACAC 
CCAGAACCAA CCATCTACTG GAAAAAGGAC AAAGTCCGAA TTGATGACAA GGAAGAGAGA 
ATAAGTATCC GTGGTGGGAA GCTGATGATC TCTAATACTA GGAAAAGCGA TGCTGGCATG 
TACACCTGTG TGGGAACCAA TATGGTGGGA GAAAGGGACA GCGACCCTGC AGAGCTCACT 
GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTGCT AGAGGAAGAA 
GCTGTAGAAT TCCGTTGTCA GGTCCAAGGA GATCCCCAGC CAACGGTGAG GTGGAAAAAA 
GATGATGCAG ACTTGCCGAG AGGAAGGTAT GATATCAAAG ATGACTACAC GCTGAGAATT 
AAAAAGGCCA TGAGTACTGA TGAAGGTACC TATGTGTGTA TTGCTGAGAA TCGGGTGGGA 
AAAGTGGAAG CCTCTGCTAC CCTCACTGTC CGAGTTCGCC CTGTTGCTCC TCCACAGTTT 
GTGGTTAGGC CAAGAGATCA GATCGTTGCT CAAGGCCGAA CAGTGACATT CCCCTGTGAA 
ACTAAAGGAA ACCCACAGCC AGCTGTTTTT TGGCAGAAAG AAGGCAGCCA GAACCTACTT 
TTCCCGAATC AACCTCAGCA GCCCAACAGC CGATGTTCAG TGTCGCCCAC GGGGGACCTC 
ACCATCACCA ACATCCAGCG TTCAGATGCG GGTTACTACA TCTGCCAGGC CCTAACCGTG 
GCAGGAAGCA TTTTAGCTAA AGCACAGTTG GAAGTTACTG ACGTTTTGAC AGATAGACCT 
CCACCCATAA TCTTGCAAGG ACCAATAAAC CAAACACTTG CAGTAGACGG TACAGCATTG 
TTGAAGTGTA AAGCCACTGG TGAGCCTCTG CCTGTAATTA GCTGGCTAAA GGAGGGCTTT 
ACTTTTCTGG GGAGAGATCC AAGAGCCACG ATCCAAGACC AAGGAACACT GCAGATTAAG 
AATTTACGGA TATCTGATAC TGGCACTTAT ACTTGTGTGG CTACAAGTTC CAGTGGAGAG 
ACTTCCTGGA GTGCAGTGCT GGATGTAACA GAATCTGGAG CAACAATCAG TAAAAATTAT 
GATATGAATG ACCTCCCGGG ACCACCATCC AAACCTCAGG TCACTGATGT TTCTAAGAAC 
AGTGTCACCT TATCCTGGCA GCCAGGTACA CCTGGCGTTC TTCCTGCAAG CGCGTATATC 
ATTGAGGCTT TCAGCCAATC GGTGAGCAAT AGCTGGCAGA CAGTGGCAAA CCATGTTAAG 
ACAACTCTGT ATACAGTAAG GGGGCTGAGG CCCAACACAA TCTACTTGTT TATGGTCAGA 
GCGATCAACC CACAAGGTCT CAGTGATCCA AGTCCTATGT CGGATCCTGT ACGCACACAA 
GATATCAGCC CCCCAGCACA AGGAGTGGAC CACAGACAGG TGCAGAAGGA ATTAGGTGAT 
GTCGTTGTTC GTCTCCATAA TCCAGTTGTC CTGACACCTA CAACTGTTCA AGTCACATGG 
ACGGTGGACC GACAACCCCA GTTTATTCAG GGCTACAGAG TGATGTACCG TCAGACTTCG 
GGACTACAAG CCTCAACTGT GTGGCAGAAT CTAGACGCCA AAGTCCCGAC TGAGAGGAGT 
GCTGTCCTTG TGAATTTGAA AAAGGGGGTG ACTTATGAAA TTAAAGTCCG GCCGTATTTT 
AACGAGTTCC AAGGAATGGA CAGTGAATCG AAAACAGTCC GAACCACTGA GGAAGCCCCA 
AGTGCCCCTC CCCAGTCTGT CACTGTGCTG ACAGTTGGAA GTCACAACAG CACAAGCATC 
AGTGTTTCCT GGGATCCTCC ACCAGCCGAC CACCAGAATG GAATTATTCA GGAATATAAG 
ATCTGGTGTC TGGGAAACGA AACGCGATTC CATATCAATA AAACGGTGGA TGCAGCCATT 
CGCTCTGTAG TAATAGGTGG CTTGTTCCCT GGAATTCAGT ACCGGGTAGA AGTGGCAGCT 
AGCACAAGTG CAGGGGTTGG AGTAAAAAGT GAACCACAGC CGATAATAAT TGGGGGACGT 
AACGAAGTTG TCATTACTGA AAACAATAAC AGCATCACTG AGCAAATCAC GGATGTCGTG 
AAGCAACCGG CATTTATAGC TGGCATTGGT GGTGCCTGCT GGGTAATTCT GATGGGTTTT 
AGCATCTGGT TGTACTGGAG AAGAAAGAAG AGAAAGGGAC TCAGCAATTA TGCTGTAACA 
TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGGAGCC GTCCAGGTCT TCTAAATGCT 
GGCGATCCCA ATTACCCATG GCTTGCTGAT TCTTGGCCAG CCACGAGTTT GCCAGTGAAC 
AATAGCAATA GTGGCCCAAA TGAAATTGGA AATTTTGGGC GTGGAGATGT GCTGCCTCCG 
GTGCCAGGCC AAGGGGATAA AACAGCGACC ATGCTCTCGG ATGGAGCCAT TTATAGCAGC 
ATTGACTTCA CTACCAAAAC CACTTACAAC AGTTCCAGCC AAATAACACA GGCCACCCCA 
TATGCCACTA CACAAATCCT GCATTCAAAC AGCATCCACG AACTGGCAGT TGATCTTCCT 
GATCCACAGT GGAAAAGCTC AGTTCAACAG AAGACAGACC TCATGGGATT TGGTTATTCG 
CTACCTGATC AGAACAAGGG GAACAACGGT GGGAAAGGTG GAAAAAAGAA GAAAACTAAA 
AATTCTTCGA AAGCGCAGAA AAACAACGGA TCCACTTGGG CTAATGTCCC TCTACCTCCT 
CCTCCCGTCC AGCCCCTTCC TGGTACAGAG CTGGGCCACT ATGCTGCGGA ACAAGAAAAT 
GGCTATGACA GTGACAGCTG GTGCCCACCA TTACCGGTGC AAACATACTT GCATCAGGGT 
ATGGAAGATG AGCTGGAAGA AGACGAAGAT CGGGTCCCAA CACCTCCTGT TCGCGGCGTG 
GCCTCTTCAC CAGCTATCTC TTTTGGACAG CAGTCCACTG CCACTCTTAC TCCATCCCCA 
CGGGAAGAGA TGCAACCCAT GCTGCAAGCT CACTTGGATG AGTTGACAAG GGCCTATCAG 
TTTGATATAG CAAAACAAAC ATGGCACATT CAAAGCAATA CCCCACCTCC ACAACCCCCA 
GCTCCGCCAT TAGGTTACGT GTCCGGAGCC TTGATTTCTG ATTTGGAGAC AGATGTTCCA 
GATGAGGATG CTGATGATGA AGAGGAACCA TTAGAAATTC CCAGGCCCCT CAGAGCACTA 
GACCAGACAC CTGGATCCAG TATGGACAAT CTAGACAGCT CTGTCACAGG AAAAGCCTTT 
AGCTCCTCTC AAAGGCAGCG GCCCACCAGC CCATTTTCTA CGGACAGTAA CACCAGTGCT 
GCCCAGAATC AAAGCCAGAG GCCTCGGCCC ACGAAAAAAC ATAAGGGAGG ACGGATGGAC 
CCACAGCCAG TGTTACCTCA TCGGAGGGAA GGGATGCCAG ATGAGGAGTC CCTGGTGCCC 
TACAGCAAGC CCAGCTTCCC GTCTCCTGGC GGGCACAGCT CATCAGGAAC ATCTTCCTCT 
AAAGGGTCCA CTGGCCCTCG GAAGGCTGAC GTGCTGAGAG GCAGCCACCA GAGGAATGCC 
AATGACCTTC TCGACATTGG ATATGTGGGC TCAAATAGTC AAGGACAGTT TACAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo2 ( XM_006523046.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523109.1 Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X14, mRNA.

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CloneID	OMu52766
Clone ID Related Accession (Same CDS sequence)	XM_006523046.3 , XM_006523046.4
Accession Version	XM_006523046.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4488bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	roundabout homolog 2 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006523046.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT 
CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG 
GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC 
CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA 
GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT 
ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG 
GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG 
CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT 
AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT 
TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC 
GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC 
CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT 
ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA 
CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA 
TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA 
GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT 
CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT 
GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG 
CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG 
GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC 
ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG 
GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT 
GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA 
GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT 
GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG 
GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT 
GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT 
CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG 
CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG 
CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT 
TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG 
GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT 
GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT 
GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGGTG GGAAAGGTGG AAAAAAGAAG 
AAAACTAAAA ATTCTTCGAA AGCGCAGAAA AACAACGGAT CCACTTGGGC TAATGTCCCT 
CTACCTCCTC CTCCCGTCCA GCCCCTTCCT GGTACAGAGC TGGGCCACTA TGCTGCGGAA 
CAAGAAAATG GCTATGACAG TGACAGCTGG TGCCCACCAT TACCGGTGCA AACATACTTG 
CATCAGGGTA TGGAAGATGA GCTGGAAGAA GACGAAGATC GGGTCCCAAC ACCTCCTGTT 
CGCGGCGTGG CCTCTTCACC AGCTATCTCT TTTGGACAGC AGTCCACTGC CACTCTTACT 
CCATCCCCAC GGGAAGAGAT GCAACCCATG CTGCAAGCTC ACTTGGATGA GTTGACAAGG 
GCCTATCAGT TTGATATAGC AAAACAAACA TGGCACATTC AAAGCAATAC CCCACCTCCA 
CAACCCCCAG CTCCGCCATT AGGTTACGTG TCCGGAGCCT TGATTTCTGA TTTGGAGACA 
GATGTTCCAG ATGAGGATGC TGATGATGAA GAGGAACCAT TAGAAATTCC CAGGCCCCTC 
AGAGCACTAG ACCAGACACC TGGATCCAGT ATGGACAATC TAGACAGCTC TGTCACAGGA 
AAAGCCTTTA GCTCCTCTCA AAGGCAGCGG CCCACCAGCC CATTTTCTAC GGACAGTAAC 
ACCAGTGCTG CCCAGAATCA AAGCCAGAGG CCTCGGCCCA CGAAAAAACA TAAGGGAGGA 
CGGATGGACC CACAGCCAGT GTTACCTCAT CGGAGGGAAG GGATGCCAGA TGATCTTCCC 
CCACCACCAG ATCCCCCACC AGGTCAGGGT TTAAGGCAGC AGATAGGCCT GAGCCAACAC 
TCTGGTAACG TGGAAAACTC AACAGAGAGA AAAGGAAGCT CTCTAGAGAG ACAACAAGCA 
GCCAACTTAG AAGACACAAA GAGCTCATTG GATTGTCCAG CTAAAACTGT TCTAGAGTGG 
CAGCGTCAAA CCCAGGACTG GATAAACTCT ACAGAACGCC AAGAAGAAAC TCGGAAAGCC 
CCACACAAAC AAGGGGTTGG ATCAGAGGAG TCCCTGGTGC CCTACAGCAA GCCCAGCTTC 
CCGTCTCCTG GCGGGCACAG CTCATCAGGA ACATCTTCCT CTAAAGGGTC CACTGGCCCT 
CGGAAGGCTG ACGTGCTGAG AGGCAGCCAC CAGAGGAATG CCAATGACCT TCTCGACATT 
GGATATGTGG GCTCAAATAG TCAAGGACAG TTTACAGGTG AATTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523109.1
CDS	9440..13927
Translation	MLSSEKVDKITRLELLFVLMLFFSGPTLGKLTRTPWKGKPWKGGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKAMSTDEGTYVCIAENRVGKVEASATLTVRVRPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPINQTLAVDGTALLKCKATGEPLPVISWLKEGFTFLGRDPRATIQDQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDMNDLPGPPSKPQVTDVSKNSVTLSWQPGTPGVLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVVVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQASTVWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSHNSTSISVSWDPPPADHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVVIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGGRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPNYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTTYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSVQQKTDLMGFGYSLPDQNKGNNGGKGGKKKKTKNSSKAQKNNGSTWANVPLPPPPVQPLPGTELGHYAAEQENGYDSDSWCPPLPVQTYLHQGMEDELEEDEDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNTPPPQPPAPPLGYVSGALISDLETDVPDEDADDEEEPLEIPRPLRALDQTPGSSMDNLDSSVTGKAFSSSQRQRPTSPFSTDSNTSAAQNQSQRPRPTKKHKGGRMDPQPVLPHRREGMPDDLPPPPDPPPGQGLRQQIGLSQHSGNVENSTERKGSSLERQQAANLEDTKSSLDCPAKTVLEWQRQTQDWINSTERQEETRKAPHKQGVGSEESLVPYSKPSFPSPGGHSSSGTSSSKGSTGPRKADVLRGSHQRNANDLLDIGYVGSNSQGQFTGEL

Target ORF information:

RefSeq Version	XM_006523046.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523046.4

ORF Insert Sequence:

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT 
CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG 
GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC 
CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA 
GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT 
ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG 
GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG 
CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT 
AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT 
TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC 
GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC 
CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT 
ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA 
CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA 
TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA 
GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT 
CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT 
GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG 
CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG 
GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC 
ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG 
GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT 
GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA 
GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT 
GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG 
GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT 
GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT 
CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG 
CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG 
CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT 
TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG 
GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT 
GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT 
GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGGTG GGAAAGGTGG AAAAAAGAAG 
AAAACTAAAA ATTCTTCGAA AGCGCAGAAA AACAACGGAT CCACTTGGGC TAATGTCCCT 
CTACCTCCTC CTCCCGTCCA GCCCCTTCCT GGTACAGAGC TGGGCCACTA TGCTGCGGAA 
CAAGAAAATG GCTATGACAG TGACAGCTGG TGCCCACCAT TACCGGTGCA AACATACTTG 
CATCAGGGTA TGGAAGATGA GCTGGAAGAA GACGAAGATC GGGTCCCAAC ACCTCCTGTT 
CGCGGCGTGG CCTCTTCACC AGCTATCTCT TTTGGACAGC AGTCCACTGC CACTCTTACT 
CCATCCCCAC GGGAAGAGAT GCAACCCATG CTGCAAGCTC ACTTGGATGA GTTGACAAGG 
GCCTATCAGT TTGATATAGC AAAACAAACA TGGCACATTC AAAGCAATAC CCCACCTCCA 
CAACCCCCAG CTCCGCCATT AGGTTACGTG TCCGGAGCCT TGATTTCTGA TTTGGAGACA 
GATGTTCCAG ATGAGGATGC TGATGATGAA GAGGAACCAT TAGAAATTCC CAGGCCCCTC 
AGAGCACTAG ACCAGACACC TGGATCCAGT ATGGACAATC TAGACAGCTC TGTCACAGGA 
AAAGCCTTTA GCTCCTCTCA AAGGCAGCGG CCCACCAGCC CATTTTCTAC GGACAGTAAC 
ACCAGTGCTG CCCAGAATCA AAGCCAGAGG CCTCGGCCCA CGAAAAAACA TAAGGGAGGA 
CGGATGGACC CACAGCCAGT GTTACCTCAT CGGAGGGAAG GGATGCCAGA TGATCTTCCC 
CCACCACCAG ATCCCCCACC AGGTCAGGGT TTAAGGCAGC AGATAGGCCT GAGCCAACAC 
TCTGGTAACG TGGAAAACTC AACAGAGAGA AAAGGAAGCT CTCTAGAGAG ACAACAAGCA 
GCCAACTTAG AAGACACAAA GAGCTCATTG GATTGTCCAG CTAAAACTGT TCTAGAGTGG 
CAGCGTCAAA CCCAGGACTG GATAAACTCT ACAGAACGCC AAGAAGAAAC TCGGAAAGCC 
CCACACAAAC AAGGGGTTGG ATCAGAGGAG TCCCTGGTGC CCTACAGCAA GCCCAGCTTC 
CCGTCTCCTG GCGGGCACAG CTCATCAGGA ACATCTTCCT CTAAAGGGTC CACTGGCCCT 
CGGAAGGCTG ACGTGCTGAG AGGCAGCCAC CAGAGGAATG CCAATGACCT TCTCGACATT 
GGATATGTGG GCTCAAATAG TCAAGGACAG TTTACAGGTG AATTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo2 ( XM_006523048.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523111.1 Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X17, mRNA.

Price & Availability↑

CloneID	OMu52768
Clone ID Related Accession (Same CDS sequence)	XM_006523048.4 , XM_006523048.3
Accession Version	XM_006523048.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4335bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	roundabout homolog 2 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006523048.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT 
CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG 
GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC 
CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA 
GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT 
ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG 
GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG 
CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT 
AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT 
TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC 
GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC 
CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT 
ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA 
CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA 
TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA 
GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT 
CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT 
GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG 
CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG 
GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC 
ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG 
GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT 
GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA 
GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT 
GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG 
GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT 
GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT 
CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG 
CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG 
CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT 
TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG 
GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT 
GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT 
GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGCCT TACTTTACAT CCCTGACTAC 
CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC 
ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA 
AAGAAGAAAA CTAAAAATTC TTCGAAAGCG CAGAAAAACA ACGGATCCAC TTGGGCTAAT 
GTCCCTCTAC CTCCTCCTCC CGTCCAGCCC CTTCCTGGTA CAGAGCTGGG CCACTATGCT 
GCGGAACAAG AAAATGGCTA TGACAGTGAC AGCTGGTGCC CACCATTACC GGTGCAAACA 
TACTTGCATC AGGGTATGGA AGATGAGCTG GAAGAAGACG AAGATCGGGT CCCAACACCT 
CCTGTTCGCG GCGTGGCCTC TTCACCAGCT ATCTCTTTTG GACAGCAGTC CACTGCCACT 
CTTACTCCAT CCCCACGGGA AGAGATGCAA CCCATGCTGC AAGCTCACTT GGATGAGTTG 
ACAAGGGCCT ATCAGTTTGA TATAGCAAAA CAAACATGGC ACATTCAAAG CAATACCCCA 
CCTCCACAAC CCCCAGCTCC GCCATTAGGT TACGTGTCCG GAGCCTTGAT TTCTGATTTG 
GAGACAGATG TTCCAGATGA GGATGCTGAT GATGAAGAGG AACCATTAGA AATTCCCAGG 
CCCCTCAGAG CACTAGACCA GACACCTGGA TCCAGTATGG ACAATCTAGA CAGCTCTGTC 
ACAGGAAAAG CCTTTAGCTC CTCTCAAAGG CAGCGGCCCA CCAGCCCATT TTCTACGGAC 
AGTAACACCA GTGCTGCCCA GAATCAAAGC CAGAGGCCTC GGCCCACGAA AAAACATAAG 
GGAGGACGGA TGGACCCACA GCCAGTGTTA CCTCATCGGA GGGAAGGGAT GCCAGATGAG 
GAGTCCCTGG TGCCCTACAG CAAGCCCAGC TTCCCGTCTC CTGGCGGGCA CAGCTCATCA 
GGAACATCTT CCTCTAAAGG GTCCACTGGC CCTCGGAAGG CTGACGTGCT GAGAGGCAGC 
CACCAGAGGA ATGCCAATGA CCTTCTCGAC ATTGGATATG TGGGCTCAAA TAGTCAAGGA 
CAGTTTACAG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523111.1
CDS	9440..13774
Translation	MLSSEKVDKITRLELLFVLMLFFSGPTLGKLTRTPWKGKPWKGGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKAMSTDEGTYVCIAENRVGKVEASATLTVRVRPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPINQTLAVDGTALLKCKATGEPLPVISWLKEGFTFLGRDPRATIQDQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDMNDLPGPPSKPQVTDVSKNSVTLSWQPGTPGVLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVVVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQASTVWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSHNSTSISVSWDPPPADHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVVIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGGRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPNYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTTYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSVQQKTDLMGFGYSLPDQNKGNNALLYIPDYRLAEGLSNRMPHNQSQDFSTTSSHNSSERSGSLSGGKGGKKKKTKNSSKAQKNNGSTWANVPLPPPPVQPLPGTELGHYAAEQENGYDSDSWCPPLPVQTYLHQGMEDELEEDEDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNTPPPQPPAPPLGYVSGALISDLETDVPDEDADDEEEPLEIPRPLRALDQTPGSSMDNLDSSVTGKAFSSSQRQRPTSPFSTDSNTSAAQNQSQRPRPTKKHKGGRMDPQPVLPHRREGMPDEESLVPYSKPSFPSPGGHSSSGTSSSKGSTGPRKADVLRGSHQRNANDLLDIGYVGSNSQGQFTE

Target ORF information:

RefSeq Version	XM_006523048.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523048.4

ORF Insert Sequence:

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT 
CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG 
GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC 
CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA 
GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT 
ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG 
GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG 
CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT 
AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT 
TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC 
GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC 
CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT 
ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA 
CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA 
TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA 
GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT 
CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT 
GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG 
CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG 
GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC 
ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG 
GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT 
GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA 
GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT 
GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG 
GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT 
GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT 
CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG 
CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG 
CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT 
TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG 
GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT 
GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT 
GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGCCT TACTTTACAT CCCTGACTAC 
CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC 
ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA 
AAGAAGAAAA CTAAAAATTC TTCGAAAGCG CAGAAAAACA ACGGATCCAC TTGGGCTAAT 
GTCCCTCTAC CTCCTCCTCC CGTCCAGCCC CTTCCTGGTA CAGAGCTGGG CCACTATGCT 
GCGGAACAAG AAAATGGCTA TGACAGTGAC AGCTGGTGCC CACCATTACC GGTGCAAACA 
TACTTGCATC AGGGTATGGA AGATGAGCTG GAAGAAGACG AAGATCGGGT CCCAACACCT 
CCTGTTCGCG GCGTGGCCTC TTCACCAGCT ATCTCTTTTG GACAGCAGTC CACTGCCACT 
CTTACTCCAT CCCCACGGGA AGAGATGCAA CCCATGCTGC AAGCTCACTT GGATGAGTTG 
ACAAGGGCCT ATCAGTTTGA TATAGCAAAA CAAACATGGC ACATTCAAAG CAATACCCCA 
CCTCCACAAC CCCCAGCTCC GCCATTAGGT TACGTGTCCG GAGCCTTGAT TTCTGATTTG 
GAGACAGATG TTCCAGATGA GGATGCTGAT GATGAAGAGG AACCATTAGA AATTCCCAGG 
CCCCTCAGAG CACTAGACCA GACACCTGGA TCCAGTATGG ACAATCTAGA CAGCTCTGTC 
ACAGGAAAAG CCTTTAGCTC CTCTCAAAGG CAGCGGCCCA CCAGCCCATT TTCTACGGAC 
AGTAACACCA GTGCTGCCCA GAATCAAAGC CAGAGGCCTC GGCCCACGAA AAAACATAAG 
GGAGGACGGA TGGACCCACA GCCAGTGTTA CCTCATCGGA GGGAAGGGAT GCCAGATGAG 
GAGTCCCTGG TGCCCTACAG CAAGCCCAGC TTCCCGTCTC CTGGCGGGCA CAGCTCATCA 
GGAACATCTT CCTCTAAAGG GTCCACTGGC CCTCGGAAGG CTGACGTGCT GAGAGGCAGC 
CACCAGAGGA ATGCCAATGA CCTTCTCGAC ATTGGATATG TGGGCTCAAA TAGTCAAGGA 
CAGTTTACAG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo2 ( XM_006523049.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523112.1 Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X19, mRNA.

Price & Availability↑

CloneID	OMu52769
Clone ID Related Accession (Same CDS sequence)	XM_006523049.3 , XM_006523049.4
Accession Version	XM_006523049.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4203bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	roundabout homolog 2 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006523049.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGCTCCTCC ACAGTTTGTG 
GTTAGGCCAA GAGATCAGAT CGTTGCTCAA GGCCGAACAG TGACATTCCC CTGTGAAACT 
AAAGGAAACC CACAGCCAGC TGTTTTTTGG CAGAAAGAAG GCAGCCAGAA CCTACTTTTC 
CCGAATCAAC CTCAGCAGCC CAACAGCCGA TGTTCAGTGT CGCCCACGGG GGACCTCACC 
ATCACCAACA TCCAGCGTTC AGATGCGGGT TACTACATCT GCCAGGCCCT AACCGTGGCA 
GGAAGCATTT TAGCTAAAGC ACAGTTGGAA GTTACTGACG TTTTGACAGA TAGACCTCCA 
CCCATAATCT TGCAAGGACC AATAAACCAA ACACTTGCAG TAGACGGTAC AGCATTGTTG 
AAGTGTAAAG CCACTGGTGA GCCTCTGCCT GTAATTAGCT GGCTAAAGGA GGGCTTTACT 
TTTCTGGGGA GAGATCCAAG AGCCACGATC CAAGACCAAG GAACACTGCA GATTAAGAAT 
TTACGGATAT CTGATACTGG CACTTATACT TGTGTGGCTA CAAGTTCCAG TGGAGAGACT 
TCCTGGAGTG CAGTGCTGGA TGTAACAGAA TCTGGAGCAA CAATCAGTAA AAATTATGAT 
ATGAATGACC TCCCGGGACC ACCATCCAAA CCTCAGGTCA CTGATGTTTC TAAGAACAGT 
GTCACCTTAT CCTGGCAGCC AGGTACACCT GGCGTTCTTC CTGCAAGCGC GTATATCATT 
GAGGCTTTCA GCCAATCGGT GAGCAATAGC TGGCAGACAG TGGCAAACCA TGTTAAGACA 
ACTCTGTATA CAGTAAGGGG GCTGAGGCCC AACACAATCT ACTTGTTTAT GGTCAGAGCG 
ATCAACCCAC AAGGTCTCAG TGATCCAAGT CCTATGTCGG ATCCTGTACG CACACAAGAT 
ATCAGCCCCC CAGCACAAGG AGTGGACCAC AGACAGGTGC AGAAGGAATT AGGTGATGTC 
GTTGTTCGTC TCCATAATCC AGTTGTCCTG ACACCTACAA CTGTTCAAGT CACATGGACG 
GTGGACCGAC AACCCCAGTT TATTCAGGGC TACAGAGTGA TGTACCGTCA GACTTCGGGA 
CTACAAGCCT CAACTGTGTG GCAGAATCTA GACGCCAAAG TCCCGACTGA GAGGAGTGCT 
GTCCTTGTGA ATTTGAAAAA GGGGGTGACT TATGAAATTA AAGTCCGGCC GTATTTTAAC 
GAGTTCCAAG GAATGGACAG TGAATCGAAA ACAGTCCGAA CCACTGAGGA AGCCCCAAGT 
GCCCCTCCCC AGTCTGTCAC TGTGCTGACA GTTGGAAGTC ACAACAGCAC AAGCATCAGT 
GTTTCCTGGG ATCCTCCACC AGCCGACCAC CAGAATGGAA TTATTCAGGA ATATAAGATC 
TGGTGTCTGG GAAACGAAAC GCGATTCCAT ATCAATAAAA CGGTGGATGC AGCCATTCGC 
TCTGTAGTAA TAGGTGGCTT GTTCCCTGGA ATTCAGTACC GGGTAGAAGT GGCAGCTAGC 
ACAAGTGCAG GGGTTGGAGT AAAAAGTGAA CCACAGCCGA TAATAATTGG GGGACGTAAC 
GAAGTTGTCA TTACTGAAAA CAATAACAGC ATCACTGAGC AAATCACGGA TGTCGTGAAG 
CAACCGGCAT TTATAGCTGG CATTGGTGGT GCCTGCTGGG TAATTCTGAT GGGTTTTAGC 
ATCTGGTTGT ACTGGAGAAG AAAGAAGAGA AAGGGACTCA GCAATTATGC TGTAACATTT 
CAAAGAGGAG ATGGAGGACT AATGAGCAAT GGGAGCCGTC CAGGTCTTCT AAATGCTGGC 
GATCCCAATT ACCCATGGCT TGCTGATTCT TGGCCAGCCA CGAGTTTGCC AGTGAACAAT 
AGCAATAGTG GCCCAAATGA AATTGGAAAT TTTGGGCGTG GAGATGTGCT GCCTCCGGTG 
CCAGGCCAAG GGGATAAAAC AGCGACCATG CTCTCGGATG GAGCCATTTA TAGCAGCATT 
GACTTCACTA CCAAAACCAC TTACAACAGT TCCAGCCAAA TAACACAGGC CACCCCATAT 
GCCACTACAC AAATCCTGCA TTCAAACAGC ATCCACGAAC TGGCAGTTGA TCTTCCTGAT 
CCACAGTGGA AAAGCTCAGT TCAACAGAAG ACAGACCTCA TGGGATTTGG TTATTCGCTA 
CCTGATCAGA ACAAGGGGAA CAACGGTGGG AAAGGTGGAA AAAAGAAGAA AACTAAAAAT 
TCTTCGAAAG CGCAGAAAAA CAACGGATCC ACTTGGGCTA ATGTCCCTCT ACCTCCTCCT 
CCCGTCCAGC CCCTTCCTGG TACAGAGCTG GGCCACTATG CTGCGGAACA AGAAAATGGC 
TATGACAGTG ACAGCTGGTG CCCACCATTA CCGGTGCAAA CATACTTGCA TCAGGGTATG 
GAAGATGAGC TGGAAGAAGA CGAAGATCGG GTCCCAACAC CTCCTGTTCG CGGCGTGGCC 
TCTTCACCAG CTATCTCTTT TGGACAGCAG TCCACTGCCA CTCTTACTCC ATCCCCACGG 
GAAGAGATGC AACCCATGCT GCAAGCTCAC TTGGATGAGT TGACAAGGGC CTATCAGTTT 
GATATAGCAA AACAAACATG GCACATTCAA AGCAATACCC CACCTCCACA ACCCCCAGCT 
CCGCCATTAG GTTACGTGTC CGGAGCCTTG ATTTCTGATT TGGAGACAGA TGTTCCAGAT 
GAGGATGCTG ATGATGAAGA GGAACCATTA GAAATTCCCA GGCCCCTCAG AGCACTAGAC 
CAGACACCTG GATCCAGTAT GGACAATCTA GACAGCTCTG TCACAGGAAA AGCCTTTAGC 
TCCTCTCAAA GGCAGCGGCC CACCAGCCCA TTTTCTACGG ACAGTAACAC CAGTGCTGCC 
CAGAATCAAA GCCAGAGGCC TCGGCCCACG AAAAAACATA AGGGAGGACG GATGGACCCA 
CAGCCAGTGT TACCTCATCG GAGGGAAGGG ATGCCAGATG AGGAGTCCCT GGTGCCCTAC 
AGCAAGCCCA GCTTCCCGTC TCCTGGCGGG CACAGCTCAT CAGGAACATC TTCCTCTAAA 
GGGTCCACTG GCCCTCGGAA GGCTGACGTG CTGAGAGGCA GCCACCAGAG GAATGCCAAT 
GACCTTCTCG ACATTGGATA TGTGGGCTCA AATAGTCAAG GACAGTTTAC AGGTGAATTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523112.1
CDS	9440..13642
Translation	MLSSEKVDKITRLELLFVLMLFFSGPTLGKLTRTPWKGKPWKGGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKAMSTDEGTYVCIAENRVGKVEASATLTVRAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPINQTLAVDGTALLKCKATGEPLPVISWLKEGFTFLGRDPRATIQDQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDMNDLPGPPSKPQVTDVSKNSVTLSWQPGTPGVLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVVVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQASTVWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSHNSTSISVSWDPPPADHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVVIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGGRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPNYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTTYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSVQQKTDLMGFGYSLPDQNKGNNGGKGGKKKKTKNSSKAQKNNGSTWANVPLPPPPVQPLPGTELGHYAAEQENGYDSDSWCPPLPVQTYLHQGMEDELEEDEDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNTPPPQPPAPPLGYVSGALISDLETDVPDEDADDEEEPLEIPRPLRALDQTPGSSMDNLDSSVTGKAFSSSQRQRPTSPFSTDSNTSAAQNQSQRPRPTKKHKGGRMDPQPVLPHRREGMPDEESLVPYSKPSFPSPGGHSSSGTSSSKGSTGPRKADVLRGSHQRNANDLLDIGYVGSNSQGQFTGEL

Target ORF information:

RefSeq Version	XM_006523049.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523049.4

ORF Insert Sequence:

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGCTCCTCC ACAGTTTGTG 
GTTAGGCCAA GAGATCAGAT CGTTGCTCAA GGCCGAACAG TGACATTCCC CTGTGAAACT 
AAAGGAAACC CACAGCCAGC TGTTTTTTGG CAGAAAGAAG GCAGCCAGAA CCTACTTTTC 
CCGAATCAAC CTCAGCAGCC CAACAGCCGA TGTTCAGTGT CGCCCACGGG GGACCTCACC 
ATCACCAACA TCCAGCGTTC AGATGCGGGT TACTACATCT GCCAGGCCCT AACCGTGGCA 
GGAAGCATTT TAGCTAAAGC ACAGTTGGAA GTTACTGACG TTTTGACAGA TAGACCTCCA 
CCCATAATCT TGCAAGGACC AATAAACCAA ACACTTGCAG TAGACGGTAC AGCATTGTTG 
AAGTGTAAAG CCACTGGTGA GCCTCTGCCT GTAATTAGCT GGCTAAAGGA GGGCTTTACT 
TTTCTGGGGA GAGATCCAAG AGCCACGATC CAAGACCAAG GAACACTGCA GATTAAGAAT 
TTACGGATAT CTGATACTGG CACTTATACT TGTGTGGCTA CAAGTTCCAG TGGAGAGACT 
TCCTGGAGTG CAGTGCTGGA TGTAACAGAA TCTGGAGCAA CAATCAGTAA AAATTATGAT 
ATGAATGACC TCCCGGGACC ACCATCCAAA CCTCAGGTCA CTGATGTTTC TAAGAACAGT 
GTCACCTTAT CCTGGCAGCC AGGTACACCT GGCGTTCTTC CTGCAAGCGC GTATATCATT 
GAGGCTTTCA GCCAATCGGT GAGCAATAGC TGGCAGACAG TGGCAAACCA TGTTAAGACA 
ACTCTGTATA CAGTAAGGGG GCTGAGGCCC AACACAATCT ACTTGTTTAT GGTCAGAGCG 
ATCAACCCAC AAGGTCTCAG TGATCCAAGT CCTATGTCGG ATCCTGTACG CACACAAGAT 
ATCAGCCCCC CAGCACAAGG AGTGGACCAC AGACAGGTGC AGAAGGAATT AGGTGATGTC 
GTTGTTCGTC TCCATAATCC AGTTGTCCTG ACACCTACAA CTGTTCAAGT CACATGGACG 
GTGGACCGAC AACCCCAGTT TATTCAGGGC TACAGAGTGA TGTACCGTCA GACTTCGGGA 
CTACAAGCCT CAACTGTGTG GCAGAATCTA GACGCCAAAG TCCCGACTGA GAGGAGTGCT 
GTCCTTGTGA ATTTGAAAAA GGGGGTGACT TATGAAATTA AAGTCCGGCC GTATTTTAAC 
GAGTTCCAAG GAATGGACAG TGAATCGAAA ACAGTCCGAA CCACTGAGGA AGCCCCAAGT 
GCCCCTCCCC AGTCTGTCAC TGTGCTGACA GTTGGAAGTC ACAACAGCAC AAGCATCAGT 
GTTTCCTGGG ATCCTCCACC AGCCGACCAC CAGAATGGAA TTATTCAGGA ATATAAGATC 
TGGTGTCTGG GAAACGAAAC GCGATTCCAT ATCAATAAAA CGGTGGATGC AGCCATTCGC 
TCTGTAGTAA TAGGTGGCTT GTTCCCTGGA ATTCAGTACC GGGTAGAAGT GGCAGCTAGC 
ACAAGTGCAG GGGTTGGAGT AAAAAGTGAA CCACAGCCGA TAATAATTGG GGGACGTAAC 
GAAGTTGTCA TTACTGAAAA CAATAACAGC ATCACTGAGC AAATCACGGA TGTCGTGAAG 
CAACCGGCAT TTATAGCTGG CATTGGTGGT GCCTGCTGGG TAATTCTGAT GGGTTTTAGC 
ATCTGGTTGT ACTGGAGAAG AAAGAAGAGA AAGGGACTCA GCAATTATGC TGTAACATTT 
CAAAGAGGAG ATGGAGGACT AATGAGCAAT GGGAGCCGTC CAGGTCTTCT AAATGCTGGC 
GATCCCAATT ACCCATGGCT TGCTGATTCT TGGCCAGCCA CGAGTTTGCC AGTGAACAAT 
AGCAATAGTG GCCCAAATGA AATTGGAAAT TTTGGGCGTG GAGATGTGCT GCCTCCGGTG 
CCAGGCCAAG GGGATAAAAC AGCGACCATG CTCTCGGATG GAGCCATTTA TAGCAGCATT 
GACTTCACTA CCAAAACCAC TTACAACAGT TCCAGCCAAA TAACACAGGC CACCCCATAT 
GCCACTACAC AAATCCTGCA TTCAAACAGC ATCCACGAAC TGGCAGTTGA TCTTCCTGAT 
CCACAGTGGA AAAGCTCAGT TCAACAGAAG ACAGACCTCA TGGGATTTGG TTATTCGCTA 
CCTGATCAGA ACAAGGGGAA CAACGGTGGG AAAGGTGGAA AAAAGAAGAA AACTAAAAAT 
TCTTCGAAAG CGCAGAAAAA CAACGGATCC ACTTGGGCTA ATGTCCCTCT ACCTCCTCCT 
CCCGTCCAGC CCCTTCCTGG TACAGAGCTG GGCCACTATG CTGCGGAACA AGAAAATGGC 
TATGACAGTG ACAGCTGGTG CCCACCATTA CCGGTGCAAA CATACTTGCA TCAGGGTATG 
GAAGATGAGC TGGAAGAAGA CGAAGATCGG GTCCCAACAC CTCCTGTTCG CGGCGTGGCC 
TCTTCACCAG CTATCTCTTT TGGACAGCAG TCCACTGCCA CTCTTACTCC ATCCCCACGG 
GAAGAGATGC AACCCATGCT GCAAGCTCAC TTGGATGAGT TGACAAGGGC CTATCAGTTT 
GATATAGCAA AACAAACATG GCACATTCAA AGCAATACCC CACCTCCACA ACCCCCAGCT 
CCGCCATTAG GTTACGTGTC CGGAGCCTTG ATTTCTGATT TGGAGACAGA TGTTCCAGAT 
GAGGATGCTG ATGATGAAGA GGAACCATTA GAAATTCCCA GGCCCCTCAG AGCACTAGAC 
CAGACACCTG GATCCAGTAT GGACAATCTA GACAGCTCTG TCACAGGAAA AGCCTTTAGC 
TCCTCTCAAA GGCAGCGGCC CACCAGCCCA TTTTCTACGG ACAGTAACAC CAGTGCTGCC 
CAGAATCAAA GCCAGAGGCC TCGGCCCACG AAAAAACATA AGGGAGGACG GATGGACCCA 
CAGCCAGTGT TACCTCATCG GAGGGAAGGG ATGCCAGATG AGGAGTCCCT GGTGCCCTAC 
AGCAAGCCCA GCTTCCCGTC TCCTGGCGGG CACAGCTCAT CAGGAACATC TTCCTCTAAA 
GGGTCCACTG GCCCTCGGAA GGCTGACGTG CTGAGAGGCA GCCACCAGAG GAATGCCAAT 
GACCTTCTCG ACATTGGATA TGTGGGCTCA AATAGTCAAG GACAGTTTAC AGGTGAATTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo2 ( XM_011246131.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244433.1 Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X15, mRNA.

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CloneID	OMu74355
Clone ID Related Accession (Same CDS sequence)	XM_011246131.2 , XM_011246131.3
Accession Version	XM_011246131.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4398bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	roundabout homolog 2 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011246131.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT 
CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG 
GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC 
CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA 
GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT 
ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG 
GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG 
CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT 
AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT 
TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC 
GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC 
CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT 
ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA 
CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA 
TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA 
GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT 
CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT 
GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG 
CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG 
GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC 
ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG 
GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT 
GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA 
GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT 
GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG 
GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT 
GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT 
CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG 
CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG 
CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT 
TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG 
GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT 
GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT 
GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGGTG GGAAAGGTGG AAAAAAGAAG 
AAAACTAAAA ATTCTTCGAA AGCGCAGAAA AACAACGGAT CCACTTGGGC TAATGTCCCT 
CTACCTCCTC CTCCCGTCCA GCCCCTTCCT GGTACAGAGC TGGGCCACTA TGCTGCGGAA 
CAAGAAAATG GCTATGACAG TGACAGCTGG TGCCCACCAT TACCGGTGCA AACATACTTG 
CATCAGGGTA TGGAAGATGA GCTGGAAGAA GACGAAGATC GGGTCCCAAC ACCTCCTGTT 
CGCGGCGTGG CCTCTTCACC AGCTATCTCT TTTGGACAGC AGTCCACTGC CACTCTTACT 
CCATCCCCAC GGGAAGAGAT GCAACCCATG CTGCAAGCTC ACTTGGATGA GTTGACAAGG 
GCCTATCAGT TTGATATAGC AAAACAAACA TGGCACATTC AAAGCAATAC CCCACCTCCA 
CAACCCCCAG CTCCGCCATT AGGTTACGTG TCCGGAGCCT TGATTTCTGA TTTGGAGACA 
GATGTTCCAG ATGAGGATGC TGATGATGAA GAGGAACCAT TAGAAATTCC CAGGCCCCTC 
AGAGCACTAG ACCAGACACC TGGATCCAGT ATGGACAATC TAGACAGCTC TGTCACAGGT 
TCAATGGTAA ATGGATGGGG CTCTGCATCT GATGAGGATC GTAACTTTTC TAGTCATAGA 
TCTAGTGTAG GTAGCTCCTC AGATGGCTCC ATCTTTGCCA GCGGCAGCTT TGCAAAAGCA 
TTGGTGGCAG CAGCAGACAA AGCTGGCTTT AGGCTGGATG GAACCAGTCT TACGAGAACA 
GGAAAAGCCT TTAGCTCCTC TCAAAGGCAG CGGCCCACCA GCCCATTTTC TACGGACAGT 
AACACCAGTG CTGCCCAGAA TCAAAGCCAG AGGCCTCGGC CCACGAAAAA ACATAAGGGA 
GGACGGATGG ACCCACAGCC AGTGTTACCT CATCGGAGGG AAGGGATGCC AGATGAGGAG 
TCCCTGGTGC CCTACAGCAA GCCCAGCTTC CCGTCTCCTG GCGGGCACAG CTCATCAGGA 
ACATCTTCCT CTAAAGGGTC CACTGGCCCT CGGAAGGCTG ACGTGCTGAG AGGCAGCCAC 
CAGAGGAATG CCAATGACCT TCTCGACATT GGATATGTGG GCTCAAATAG TCAAGGACAG 
TTTACAGGTG AATTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244433.1
CDS	9440..13837
Translation	MLSSEKVDKITRLELLFVLMLFFSGPTLGKLTRTPWKGKPWKGGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKAMSTDEGTYVCIAENRVGKVEASATLTVRVRPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPINQTLAVDGTALLKCKATGEPLPVISWLKEGFTFLGRDPRATIQDQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDMNDLPGPPSKPQVTDVSKNSVTLSWQPGTPGVLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVVVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQASTVWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSHNSTSISVSWDPPPADHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVVIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGGRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPNYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTTYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSVQQKTDLMGFGYSLPDQNKGNNGGKGGKKKKTKNSSKAQKNNGSTWANVPLPPPPVQPLPGTELGHYAAEQENGYDSDSWCPPLPVQTYLHQGMEDELEEDEDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNTPPPQPPAPPLGYVSGALISDLETDVPDEDADDEEEPLEIPRPLRALDQTPGSSMDNLDSSVTGSMVNGWGSASDEDRNFSSHRSSVGSSSDGSIFASGSFAKALVAAADKAGFRLDGTSLTRTGKAFSSSQRQRPTSPFSTDSNTSAAQNQSQRPRPTKKHKGGRMDPQPVLPHRREGMPDEESLVPYSKPSFPSPGGHSSSGTSSSKGSTGPRKADVLRGSHQRNANDLLDIGYVGSNSQGQFTGEL

Target ORF information:

RefSeq Version	XM_011246131.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011246131.3

ORF Insert Sequence:

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT 
CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG 
GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC 
CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA 
GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT 
ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG 
GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG 
CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT 
AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT 
TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC 
GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC 
CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT 
ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA 
CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA 
TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA 
GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT 
CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT 
GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG 
CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG 
GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC 
ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG 
GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT 
GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA 
GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT 
GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG 
GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT 
GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT 
CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG 
CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG 
CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT 
TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG 
GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT 
GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT 
GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGGTG GGAAAGGTGG AAAAAAGAAG 
AAAACTAAAA ATTCTTCGAA AGCGCAGAAA AACAACGGAT CCACTTGGGC TAATGTCCCT 
CTACCTCCTC CTCCCGTCCA GCCCCTTCCT GGTACAGAGC TGGGCCACTA TGCTGCGGAA 
CAAGAAAATG GCTATGACAG TGACAGCTGG TGCCCACCAT TACCGGTGCA AACATACTTG 
CATCAGGGTA TGGAAGATGA GCTGGAAGAA GACGAAGATC GGGTCCCAAC ACCTCCTGTT 
CGCGGCGTGG CCTCTTCACC AGCTATCTCT TTTGGACAGC AGTCCACTGC CACTCTTACT 
CCATCCCCAC GGGAAGAGAT GCAACCCATG CTGCAAGCTC ACTTGGATGA GTTGACAAGG 
GCCTATCAGT TTGATATAGC AAAACAAACA TGGCACATTC AAAGCAATAC CCCACCTCCA 
CAACCCCCAG CTCCGCCATT AGGTTACGTG TCCGGAGCCT TGATTTCTGA TTTGGAGACA 
GATGTTCCAG ATGAGGATGC TGATGATGAA GAGGAACCAT TAGAAATTCC CAGGCCCCTC 
AGAGCACTAG ACCAGACACC TGGATCCAGT ATGGACAATC TAGACAGCTC TGTCACAGGT 
TCAATGGTAA ATGGATGGGG CTCTGCATCT GATGAGGATC GTAACTTTTC TAGTCATAGA 
TCTAGTGTAG GTAGCTCCTC AGATGGCTCC ATCTTTGCCA GCGGCAGCTT TGCAAAAGCA 
TTGGTGGCAG CAGCAGACAA AGCTGGCTTT AGGCTGGATG GAACCAGTCT TACGAGAACA 
GGAAAAGCCT TTAGCTCCTC TCAAAGGCAG CGGCCCACCA GCCCATTTTC TACGGACAGT 
AACACCAGTG CTGCCCAGAA TCAAAGCCAG AGGCCTCGGC CCACGAAAAA ACATAAGGGA 
GGACGGATGG ACCCACAGCC AGTGTTACCT CATCGGAGGG AAGGGATGCC AGATGAGGAG 
TCCCTGGTGC CCTACAGCAA GCCCAGCTTC CCGTCTCCTG GCGGGCACAG CTCATCAGGA 
ACATCTTCCT CTAAAGGGTC CACTGGCCCT CGGAAGGCTG ACGTGCTGAG AGGCAGCCAC 
CAGAGGAATG CCAATGACCT TCTCGACATT GGATATGTGG GCTCAAATAG TCAAGGACAG 
TTTACAGGTG AATTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo2 ( XM_017317015.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172504.1 Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X16, mRNA.

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CloneID	OMu79342
Clone ID Related Accession (Same CDS sequence)	XM_017317015.1 , XM_017317015.2
Accession Version	XM_017317015.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4341bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	roundabout homolog 2 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT 
CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG 
GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC 
CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA 
GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT 
ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG 
GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG 
CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT 
AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT 
TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC 
GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC 
CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT 
ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA 
CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA 
TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA 
GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT 
CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT 
GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG 
CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG 
GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC 
ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG 
GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT 
GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA 
GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT 
GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG 
GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT 
GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT 
CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG 
CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG 
CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT 
TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG 
GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT 
GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT 
GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGCCT TACTTTACAT CCCTGACTAC 
CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC 
ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA 
AAGAAGAAAA CTAAAAATTC TTCGAAAGCG CAGAAAAACA ACGGATCCAC TTGGGCTAAT 
GTCCCTCTAC CTCCTCCTCC CGTCCAGCCC CTTCCTGGTA CAGAGCTGGG CCACTATGCT 
GCGGAACAAG AAAATGGCTA TGACAGTGAC AGCTGGTGCC CACCATTACC GGTGCAAACA 
TACTTGCATC AGGGTATGGA AGATGAGCTG GAAGAAGACG AAGATCGGGT CCCAACACCT 
CCTGTTCGCG GCGTGGCCTC TTCACCAGCT ATCTCTTTTG GACAGCAGTC CACTGCCACT 
CTTACTCCAT CCCCACGGGA AGAGATGCAA CCCATGCTGC AAGCTCACTT GGATGAGTTG 
ACAAGGGCCT ATCAGTTTGA TATAGCAAAA CAAACATGGC ACATTCAAAG CAATACCCCA 
CCTCCACAAC CCCCAGCTCC GCCATTAGGT TACGTGTCCG GAGCCTTGAT TTCTGATTTG 
GAGACAGATG TTCCAGATGA GGATGCTGAT GATGAAGAGG AACCATTAGA AATTCCCAGG 
CCCCTCAGAG CACTAGACCA GACACCTGGA TCCAGTATGG ACAATCTAGA CAGCTCTGTC 
ACAGGAAAAG CCTTTAGCTC CTCTCAAAGG CAGCGGCCCA CCAGCCCATT TTCTACGGAC 
AGTAACACCA GTGCTGCCCA GAATCAAAGC CAGAGGCCTC GGCCCACGAA AAAACATAAG 
GGAGGACGGA TGGACCCACA GCCAGTGTTA CCTCATCGGA GGGAAGGGAT GCCAGATGAG 
GAGTCCCTGG TGCCCTACAG CAAGCCCAGC TTCCCGTCTC CTGGCGGGCA CAGCTCATCA 
GGAACATCTT CCTCTAAAGG GTCCACTGGC CCTCGGAAGG CTGACGTGCT GAGAGGCAGC 
CACCAGAGGA ATGCCAATGA CCTTCTCGAC ATTGGATATG TGGGCTCAAA TAGTCAAGGA 
CAGTTTACAG GTGAATTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172504.1
CDS	337..4677
Translation	MLSSEKVDKITRLELLFVLMLFFSGPTLGKLTRTPWKGKPWKGGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKAMSTDEGTYVCIAENRVGKVEASATLTVRVRPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPINQTLAVDGTALLKCKATGEPLPVISWLKEGFTFLGRDPRATIQDQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDMNDLPGPPSKPQVTDVSKNSVTLSWQPGTPGVLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVVVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQASTVWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSHNSTSISVSWDPPPADHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVVIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGGRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPNYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTTYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSVQQKTDLMGFGYSLPDQNKGNNALLYIPDYRLAEGLSNRMPHNQSQDFSTTSSHNSSERSGSLSGGKGGKKKKTKNSSKAQKNNGSTWANVPLPPPPVQPLPGTELGHYAAEQENGYDSDSWCPPLPVQTYLHQGMEDELEEDEDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNTPPPQPPAPPLGYVSGALISDLETDVPDEDADDEEEPLEIPRPLRALDQTPGSSMDNLDSSVTGKAFSSSQRQRPTSPFSTDSNTSAAQNQSQRPRPTKKHKGGRMDPQPVLPHRREGMPDEESLVPYSKPSFPSPGGHSSSGTSSSKGSTGPRKADVLRGSHQRNANDLLDIGYVGSNSQGQFTGEL

Target ORF information:

RefSeq Version	XM_017317015.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317015.1

ORF Insert Sequence:

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT 
CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG 
GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC 
CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA 
GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT 
ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG 
GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG 
CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT 
AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT 
TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC 
GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC 
CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT 
ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA 
CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA 
TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA 
GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT 
CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT 
GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG 
CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG 
GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC 
ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG 
GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT 
GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA 
GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT 
GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG 
GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT 
GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT 
CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG 
CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG 
CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT 
TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG 
GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT 
GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT 
GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGCCT TACTTTACAT CCCTGACTAC 
CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC 
ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA 
AAGAAGAAAA CTAAAAATTC TTCGAAAGCG CAGAAAAACA ACGGATCCAC TTGGGCTAAT 
GTCCCTCTAC CTCCTCCTCC CGTCCAGCCC CTTCCTGGTA CAGAGCTGGG CCACTATGCT 
GCGGAACAAG AAAATGGCTA TGACAGTGAC AGCTGGTGCC CACCATTACC GGTGCAAACA 
TACTTGCATC AGGGTATGGA AGATGAGCTG GAAGAAGACG AAGATCGGGT CCCAACACCT 
CCTGTTCGCG GCGTGGCCTC TTCACCAGCT ATCTCTTTTG GACAGCAGTC CACTGCCACT 
CTTACTCCAT CCCCACGGGA AGAGATGCAA CCCATGCTGC AAGCTCACTT GGATGAGTTG 
ACAAGGGCCT ATCAGTTTGA TATAGCAAAA CAAACATGGC ACATTCAAAG CAATACCCCA 
CCTCCACAAC CCCCAGCTCC GCCATTAGGT TACGTGTCCG GAGCCTTGAT TTCTGATTTG 
GAGACAGATG TTCCAGATGA GGATGCTGAT GATGAAGAGG AACCATTAGA AATTCCCAGG 
CCCCTCAGAG CACTAGACCA GACACCTGGA TCCAGTATGG ACAATCTAGA CAGCTCTGTC 
ACAGGAAAAG CCTTTAGCTC CTCTCAAAGG CAGCGGCCCA CCAGCCCATT TTCTACGGAC 
AGTAACACCA GTGCTGCCCA GAATCAAAGC CAGAGGCCTC GGCCCACGAA AAAACATAAG 
GGAGGACGGA TGGACCCACA GCCAGTGTTA CCTCATCGGA GGGAAGGGAT GCCAGATGAG 
GAGTCCCTGG TGCCCTACAG CAAGCCCAGC TTCCCGTCTC CTGGCGGGCA CAGCTCATCA 
GGAACATCTT CCTCTAAAGG GTCCACTGGC CCTCGGAAGG CTGACGTGCT GAGAGGCAGC 
CACCAGAGGA ATGCCAATGA CCTTCTCGAC ATTGGATATG TGGGCTCAAA TAGTCAAGGA 
CAGTTTACAG GTGAATTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo2 ( XM_017317015.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172504.1 Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OMu79342
Clone ID Related Accession (Same CDS sequence)	XM_017317015.1 , XM_017317015.2
Accession Version	XM_017317015.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4341bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	roundabout homolog 2 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017317015.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT 
CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG 
GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC 
CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA 
GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT 
ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG 
GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG 
CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT 
AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT 
TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC 
GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC 
CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT 
ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA 
CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA 
TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA 
GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT 
CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT 
GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG 
CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG 
GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC 
ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG 
GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT 
GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA 
GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT 
GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG 
GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT 
GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT 
CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG 
CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG 
CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT 
TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG 
GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT 
GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT 
GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGCCT TACTTTACAT CCCTGACTAC 
CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC 
ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA 
AAGAAGAAAA CTAAAAATTC TTCGAAAGCG CAGAAAAACA ACGGATCCAC TTGGGCTAAT 
GTCCCTCTAC CTCCTCCTCC CGTCCAGCCC CTTCCTGGTA CAGAGCTGGG CCACTATGCT 
GCGGAACAAG AAAATGGCTA TGACAGTGAC AGCTGGTGCC CACCATTACC GGTGCAAACA 
TACTTGCATC AGGGTATGGA AGATGAGCTG GAAGAAGACG AAGATCGGGT CCCAACACCT 
CCTGTTCGCG GCGTGGCCTC TTCACCAGCT ATCTCTTTTG GACAGCAGTC CACTGCCACT 
CTTACTCCAT CCCCACGGGA AGAGATGCAA CCCATGCTGC AAGCTCACTT GGATGAGTTG 
ACAAGGGCCT ATCAGTTTGA TATAGCAAAA CAAACATGGC ACATTCAAAG CAATACCCCA 
CCTCCACAAC CCCCAGCTCC GCCATTAGGT TACGTGTCCG GAGCCTTGAT TTCTGATTTG 
GAGACAGATG TTCCAGATGA GGATGCTGAT GATGAAGAGG AACCATTAGA AATTCCCAGG 
CCCCTCAGAG CACTAGACCA GACACCTGGA TCCAGTATGG ACAATCTAGA CAGCTCTGTC 
ACAGGAAAAG CCTTTAGCTC CTCTCAAAGG CAGCGGCCCA CCAGCCCATT TTCTACGGAC 
AGTAACACCA GTGCTGCCCA GAATCAAAGC CAGAGGCCTC GGCCCACGAA AAAACATAAG 
GGAGGACGGA TGGACCCACA GCCAGTGTTA CCTCATCGGA GGGAAGGGAT GCCAGATGAG 
GAGTCCCTGG TGCCCTACAG CAAGCCCAGC TTCCCGTCTC CTGGCGGGCA CAGCTCATCA 
GGAACATCTT CCTCTAAAGG GTCCACTGGC CCTCGGAAGG CTGACGTGCT GAGAGGCAGC 
CACCAGAGGA ATGCCAATGA CCTTCTCGAC ATTGGATATG TGGGCTCAAA TAGTCAAGGA 
CAGTTTACAG GTGAATTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172504.1
CDS	9440..13780
Translation	MLSSEKVDKITRLELLFVLMLFFSGPTLGKLTRTPWKGKPWKGGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKAMSTDEGTYVCIAENRVGKVEASATLTVRVRPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPINQTLAVDGTALLKCKATGEPLPVISWLKEGFTFLGRDPRATIQDQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDMNDLPGPPSKPQVTDVSKNSVTLSWQPGTPGVLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVVVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQASTVWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSHNSTSISVSWDPPPADHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVVIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGGRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPNYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTTYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSVQQKTDLMGFGYSLPDQNKGNNALLYIPDYRLAEGLSNRMPHNQSQDFSTTSSHNSSERSGSLSGGKGGKKKKTKNSSKAQKNNGSTWANVPLPPPPVQPLPGTELGHYAAEQENGYDSDSWCPPLPVQTYLHQGMEDELEEDEDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNTPPPQPPAPPLGYVSGALISDLETDVPDEDADDEEEPLEIPRPLRALDQTPGSSMDNLDSSVTGKAFSSSQRQRPTSPFSTDSNTSAAQNQSQRPRPTKKHKGGRMDPQPVLPHRREGMPDEESLVPYSKPSFPSPGGHSSSGTSSSKGSTGPRKADVLRGSHQRNANDLLDIGYVGSNSQGQFTGEL

Target ORF information:

RefSeq Version	XM_017317015.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317015.2

ORF Insert Sequence:

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT 
CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG 
GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC 
CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA 
GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT 
ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG 
GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG 
CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT 
AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT 
TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC 
GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC 
CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT 
ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA 
CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA 
TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA 
GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT 
CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT 
GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG 
CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG 
GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC 
ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG 
GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT 
GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA 
GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT 
GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG 
GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT 
GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT 
CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG 
CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG 
CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT 
TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG 
GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT 
GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT 
GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGCCT TACTTTACAT CCCTGACTAC 
CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC 
ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA 
AAGAAGAAAA CTAAAAATTC TTCGAAAGCG CAGAAAAACA ACGGATCCAC TTGGGCTAAT 
GTCCCTCTAC CTCCTCCTCC CGTCCAGCCC CTTCCTGGTA CAGAGCTGGG CCACTATGCT 
GCGGAACAAG AAAATGGCTA TGACAGTGAC AGCTGGTGCC CACCATTACC GGTGCAAACA 
TACTTGCATC AGGGTATGGA AGATGAGCTG GAAGAAGACG AAGATCGGGT CCCAACACCT 
CCTGTTCGCG GCGTGGCCTC TTCACCAGCT ATCTCTTTTG GACAGCAGTC CACTGCCACT 
CTTACTCCAT CCCCACGGGA AGAGATGCAA CCCATGCTGC AAGCTCACTT GGATGAGTTG 
ACAAGGGCCT ATCAGTTTGA TATAGCAAAA CAAACATGGC ACATTCAAAG CAATACCCCA 
CCTCCACAAC CCCCAGCTCC GCCATTAGGT TACGTGTCCG GAGCCTTGAT TTCTGATTTG 
GAGACAGATG TTCCAGATGA GGATGCTGAT GATGAAGAGG AACCATTAGA AATTCCCAGG 
CCCCTCAGAG CACTAGACCA GACACCTGGA TCCAGTATGG ACAATCTAGA CAGCTCTGTC 
ACAGGAAAAG CCTTTAGCTC CTCTCAAAGG CAGCGGCCCA CCAGCCCATT TTCTACGGAC 
AGTAACACCA GTGCTGCCCA GAATCAAAGC CAGAGGCCTC GGCCCACGAA AAAACATAAG 
GGAGGACGGA TGGACCCACA GCCAGTGTTA CCTCATCGGA GGGAAGGGAT GCCAGATGAG 
GAGTCCCTGG TGCCCTACAG CAAGCCCAGC TTCCCGTCTC CTGGCGGGCA CAGCTCATCA 
GGAACATCTT CCTCTAAAGG GTCCACTGGC CCTCGGAAGG CTGACGTGCT GAGAGGCAGC 
CACCAGAGGA ATGCCAATGA CCTTCTCGAC ATTGGATATG TGGGCTCAAA TAGTCAAGGA 
CAGTTTACAG GTGAATTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo2 ( XM_017317016.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172505.1 Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X18, mRNA.

Price & Availability↑

CloneID	OMu79343
Clone ID Related Accession (Same CDS sequence)	XM_017317016.2 , XM_017317016.1
Accession Version	XM_017317016.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4209bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	roundabout homolog 2 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017317016.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT 
CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG 
GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC 
CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA 
GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT 
ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG 
GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG 
CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT 
AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT 
TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC 
GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC 
CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT 
ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA 
CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA 
TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA 
GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT 
CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT 
GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG 
CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG 
GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC 
ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG 
GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT 
GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA 
GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT 
GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG 
GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT 
GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT 
CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG 
CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG 
CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT 
TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG 
GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT 
GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT 
GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGGTG GGAAAGGTGG AAAAAAGAAG 
AAAACTAAAA ATTCTTCGAA AGCGCAGAAA AACAACGGAT CCACTTGGGC TAATGTCCCT 
CTACCTCCTC CTCCCGTCCA GCCCCTTCCT GGTACAGAGC TGGGCCACTA TGCTGCGGAA 
CAAGAAAATG GCTATGACAG TGACAGCTGG TGCCCACCAT TACCGGTGCA AACATACTTG 
CATCAGGGTA TGGAAGATGA GCTGGAAGAA GACGAAGATC GGGTCCCAAC ACCTCCTGTT 
CGCGGCGTGG CCTCTTCACC AGCTATCTCT TTTGGACAGC AGTCCACTGC CACTCTTACT 
CCATCCCCAC GGGAAGAGAT GCAACCCATG CTGCAAGCTC ACTTGGATGA GTTGACAAGG 
GCCTATCAGT TTGATATAGC AAAACAAACA TGGCACATTC AAAGCAATAC CCCACCTCCA 
CAACCCCCAG CTCCGCCATT AGGTTACGTG TCCGGAGCCT TGATTTCTGA TTTGGAGACA 
GATGTTCCAG ATGAGGATGC TGATGATGAA GAGGAACCAT TAGAAATTCC CAGGCCCCTC 
AGAGCACTAG ACCAGACACC TGGATCCAGT ATGGACAATC TAGACAGCTC TGTCACAGGA 
AAAGCCTTTA GCTCCTCTCA AAGGCAGCGG CCCACCAGCC CATTTTCTAC GGACAGTAAC 
ACCAGTGCTG CCCAGAATCA AAGCCAGAGG CCTCGGCCCA CGAAAAAACA TAAGGGAGGA 
CGGATGGACC CACAGCCAGT GTTACCTCAT CGGAGGGAAG GGATGCCAGA TGAGGAGTCC 
CTGGTGCCCT ACAGCAAGCC CAGCTTCCCG TCTCCTGGCG GGCACAGCTC ATCAGGAACA 
TCTTCCTCTA AAGGGTCCAC TGGCCCTCGG AAGGCTGACG TGCTGAGAGG CAGCCACCAG 
AGGAATGCCA ATGACCTTCT CGACATTGGA TATGTGGGCT CAAATAGTCA AGGACAGTTT 
ACAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172505.1
CDS	9440..13648
Translation	MLSSEKVDKITRLELLFVLMLFFSGPTLGKLTRTPWKGKPWKGGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKAMSTDEGTYVCIAENRVGKVEASATLTVRVRPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPINQTLAVDGTALLKCKATGEPLPVISWLKEGFTFLGRDPRATIQDQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDMNDLPGPPSKPQVTDVSKNSVTLSWQPGTPGVLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVVVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQASTVWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSHNSTSISVSWDPPPADHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVVIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGGRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPNYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTTYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSVQQKTDLMGFGYSLPDQNKGNNGGKGGKKKKTKNSSKAQKNNGSTWANVPLPPPPVQPLPGTELGHYAAEQENGYDSDSWCPPLPVQTYLHQGMEDELEEDEDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNTPPPQPPAPPLGYVSGALISDLETDVPDEDADDEEEPLEIPRPLRALDQTPGSSMDNLDSSVTGKAFSSSQRQRPTSPFSTDSNTSAAQNQSQRPRPTKKHKGGRMDPQPVLPHRREGMPDEESLVPYSKPSFPSPGGHSSSGTSSSKGSTGPRKADVLRGSHQRNANDLLDIGYVGSNSQGQFTE

Target ORF information:

RefSeq Version	XM_017317016.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317016.2

ORF Insert Sequence:

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT 
CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG 
GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC 
CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA 
GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT 
ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG 
GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG 
CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT 
AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT 
TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC 
GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC 
CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT 
ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA 
CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA 
TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA 
GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT 
CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT 
GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG 
CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG 
GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC 
ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG 
GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT 
GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA 
GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT 
GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG 
GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT 
GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT 
CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG 
CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG 
CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT 
TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG 
GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT 
GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT 
GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGGTG GGAAAGGTGG AAAAAAGAAG 
AAAACTAAAA ATTCTTCGAA AGCGCAGAAA AACAACGGAT CCACTTGGGC TAATGTCCCT 
CTACCTCCTC CTCCCGTCCA GCCCCTTCCT GGTACAGAGC TGGGCCACTA TGCTGCGGAA 
CAAGAAAATG GCTATGACAG TGACAGCTGG TGCCCACCAT TACCGGTGCA AACATACTTG 
CATCAGGGTA TGGAAGATGA GCTGGAAGAA GACGAAGATC GGGTCCCAAC ACCTCCTGTT 
CGCGGCGTGG CCTCTTCACC AGCTATCTCT TTTGGACAGC AGTCCACTGC CACTCTTACT 
CCATCCCCAC GGGAAGAGAT GCAACCCATG CTGCAAGCTC ACTTGGATGA GTTGACAAGG 
GCCTATCAGT TTGATATAGC AAAACAAACA TGGCACATTC AAAGCAATAC CCCACCTCCA 
CAACCCCCAG CTCCGCCATT AGGTTACGTG TCCGGAGCCT TGATTTCTGA TTTGGAGACA 
GATGTTCCAG ATGAGGATGC TGATGATGAA GAGGAACCAT TAGAAATTCC CAGGCCCCTC 
AGAGCACTAG ACCAGACACC TGGATCCAGT ATGGACAATC TAGACAGCTC TGTCACAGGA 
AAAGCCTTTA GCTCCTCTCA AAGGCAGCGG CCCACCAGCC CATTTTCTAC GGACAGTAAC 
ACCAGTGCTG CCCAGAATCA AAGCCAGAGG CCTCGGCCCA CGAAAAAACA TAAGGGAGGA 
CGGATGGACC CACAGCCAGT GTTACCTCAT CGGAGGGAAG GGATGCCAGA TGAGGAGTCC 
CTGGTGCCCT ACAGCAAGCC CAGCTTCCCG TCTCCTGGCG GGCACAGCTC ATCAGGAACA 
TCTTCCTCTA AAGGGTCCAC TGGCCCTCGG AAGGCTGACG TGCTGAGAGG CAGCCACCAG 
AGGAATGCCA ATGACCTTCT CGACATTGGA TATGTGGGCT CAAATAGTCA AGGACAGTTT 
ACAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo2 ( XM_017317016.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172505.1 Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X19, mRNA.

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CloneID	OMu79343
Clone ID Related Accession (Same CDS sequence)	XM_017317016.2 , XM_017317016.1
Accession Version	XM_017317016.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4209bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	roundabout homolog 2 isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT 
CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG 
GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC 
CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA 
GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT 
ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG 
GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG 
CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT 
AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT 
TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC 
GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC 
CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT 
ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA 
CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA 
TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA 
GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT 
CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT 
GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG 
CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG 
GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC 
ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG 
GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT 
GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA 
GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT 
GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG 
GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT 
GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT 
CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG 
CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG 
CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT 
TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG 
GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT 
GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT 
GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGGTG GGAAAGGTGG AAAAAAGAAG 
AAAACTAAAA ATTCTTCGAA AGCGCAGAAA AACAACGGAT CCACTTGGGC TAATGTCCCT 
CTACCTCCTC CTCCCGTCCA GCCCCTTCCT GGTACAGAGC TGGGCCACTA TGCTGCGGAA 
CAAGAAAATG GCTATGACAG TGACAGCTGG TGCCCACCAT TACCGGTGCA AACATACTTG 
CATCAGGGTA TGGAAGATGA GCTGGAAGAA GACGAAGATC GGGTCCCAAC ACCTCCTGTT 
CGCGGCGTGG CCTCTTCACC AGCTATCTCT TTTGGACAGC AGTCCACTGC CACTCTTACT 
CCATCCCCAC GGGAAGAGAT GCAACCCATG CTGCAAGCTC ACTTGGATGA GTTGACAAGG 
GCCTATCAGT TTGATATAGC AAAACAAACA TGGCACATTC AAAGCAATAC CCCACCTCCA 
CAACCCCCAG CTCCGCCATT AGGTTACGTG TCCGGAGCCT TGATTTCTGA TTTGGAGACA 
GATGTTCCAG ATGAGGATGC TGATGATGAA GAGGAACCAT TAGAAATTCC CAGGCCCCTC 
AGAGCACTAG ACCAGACACC TGGATCCAGT ATGGACAATC TAGACAGCTC TGTCACAGGA 
AAAGCCTTTA GCTCCTCTCA AAGGCAGCGG CCCACCAGCC CATTTTCTAC GGACAGTAAC 
ACCAGTGCTG CCCAGAATCA AAGCCAGAGG CCTCGGCCCA CGAAAAAACA TAAGGGAGGA 
CGGATGGACC CACAGCCAGT GTTACCTCAT CGGAGGGAAG GGATGCCAGA TGAGGAGTCC 
CTGGTGCCCT ACAGCAAGCC CAGCTTCCCG TCTCCTGGCG GGCACAGCTC ATCAGGAACA 
TCTTCCTCTA AAGGGTCCAC TGGCCCTCGG AAGGCTGACG TGCTGAGAGG CAGCCACCAG 
AGGAATGCCA ATGACCTTCT CGACATTGGA TATGTGGGCT CAAATAGTCA AGGACAGTTT 
ACAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172505.1
CDS	337..4545
Translation	MLSSEKVDKITRLELLFVLMLFFSGPTLGKLTRTPWKGKPWKGGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKAMSTDEGTYVCIAENRVGKVEASATLTVRVRPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPINQTLAVDGTALLKCKATGEPLPVISWLKEGFTFLGRDPRATIQDQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDMNDLPGPPSKPQVTDVSKNSVTLSWQPGTPGVLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVVVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQASTVWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSHNSTSISVSWDPPPADHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVVIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGGRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPNYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTTYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSVQQKTDLMGFGYSLPDQNKGNNGGKGGKKKKTKNSSKAQKNNGSTWANVPLPPPPVQPLPGTELGHYAAEQENGYDSDSWCPPLPVQTYLHQGMEDELEEDEDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNTPPPQPPAPPLGYVSGALISDLETDVPDEDADDEEEPLEIPRPLRALDQTPGSSMDNLDSSVTGKAFSSSQRQRPTSPFSTDSNTSAAQNQSQRPRPTKKHKGGRMDPQPVLPHRREGMPDEESLVPYSKPSFPSPGGHSSSGTSSSKGSTGPRKADVLRGSHQRNANDLLDIGYVGSNSQGQFTE

Target ORF information:

RefSeq Version	XM_017317016.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317016.1

ORF Insert Sequence:

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT 
CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG 
GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC 
CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA 
GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT 
ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG 
GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG 
CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT 
AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT 
TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC 
GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC 
CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT 
ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA 
CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA 
TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA 
GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT 
CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT 
GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG 
CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG 
GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC 
ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG 
GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT 
GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA 
GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT 
GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG 
GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT 
GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT 
CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG 
CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG 
CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT 
TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG 
GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT 
GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT 
GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGGTG GGAAAGGTGG AAAAAAGAAG 
AAAACTAAAA ATTCTTCGAA AGCGCAGAAA AACAACGGAT CCACTTGGGC TAATGTCCCT 
CTACCTCCTC CTCCCGTCCA GCCCCTTCCT GGTACAGAGC TGGGCCACTA TGCTGCGGAA 
CAAGAAAATG GCTATGACAG TGACAGCTGG TGCCCACCAT TACCGGTGCA AACATACTTG 
CATCAGGGTA TGGAAGATGA GCTGGAAGAA GACGAAGATC GGGTCCCAAC ACCTCCTGTT 
CGCGGCGTGG CCTCTTCACC AGCTATCTCT TTTGGACAGC AGTCCACTGC CACTCTTACT 
CCATCCCCAC GGGAAGAGAT GCAACCCATG CTGCAAGCTC ACTTGGATGA GTTGACAAGG 
GCCTATCAGT TTGATATAGC AAAACAAACA TGGCACATTC AAAGCAATAC CCCACCTCCA 
CAACCCCCAG CTCCGCCATT AGGTTACGTG TCCGGAGCCT TGATTTCTGA TTTGGAGACA 
GATGTTCCAG ATGAGGATGC TGATGATGAA GAGGAACCAT TAGAAATTCC CAGGCCCCTC 
AGAGCACTAG ACCAGACACC TGGATCCAGT ATGGACAATC TAGACAGCTC TGTCACAGGA 
AAAGCCTTTA GCTCCTCTCA AAGGCAGCGG CCCACCAGCC CATTTTCTAC GGACAGTAAC 
ACCAGTGCTG CCCAGAATCA AAGCCAGAGG CCTCGGCCCA CGAAAAAACA TAAGGGAGGA 
CGGATGGACC CACAGCCAGT GTTACCTCAT CGGAGGGAAG GGATGCCAGA TGAGGAGTCC 
CTGGTGCCCT ACAGCAAGCC CAGCTTCCCG TCTCCTGGCG GGCACAGCTC ATCAGGAACA 
TCTTCCTCTA AAGGGTCCAC TGGCCCTCGG AAGGCTGACG TGCTGAGAGG CAGCCACCAG 
AGGAATGCCA ATGACCTTCT CGACATTGGA TATGTGGGCT CAAATAGTCA AGGACAGTTT 
ACAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo2 ( XM_017317017.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172506.1 Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X21, mRNA.

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CloneID	OMu79344
Clone ID Related Accession (Same CDS sequence)	XM_017317017.1
Accession Version	XM_017317017.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4185bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	roundabout homolog 2 isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGAA GACAAGAAAG TATCTGTGGA AGGCCGTGGA CGTGGACGCC AGGACTACTG 
ATGCTGATCA TCCTTGGCAT TCATCAGGGG AGTGGGCAAG GTCAAGGATC TCGTCTTCGT 
CAAGAAGACT TTCCCCCCAG GATCGTAGAA CACCCTTCTG ATGTCATCGT CTCCAAGGGA 
GAGCCCACCA CTCTGAACTG TAAAGCAGAG GGCCGACCCA CCCCCACCAT TGAATGGTAC 
AAGGATGGTG AGAGGGTGGA GACAGACAAG GATGATCCCA GGTCCCACAG AATGCTTCTG 
CCCAGCGGAT CTTTATTCTT TTTGCGAATT GTTCATGGGC GCAGAAGTAA ACCGGACGAA 
GGGAGTTACG TTTGTGTTGC AAGGAACTAT CTTGGTGAAG CAGTGAGTCG AAATGCATCT 
CTGGAAGTGG CATTATTGCG AGATGACTTC CGGCAAAACC CCACAGATGT GGTAGTCGCA 
GCTGGAGAGC CTGCAATCTT GGAGTGCCAG CCACCACGGG GACACCCAGA ACCAACCATC 
TACTGGAAAA AGGACAAAGT CCGAATTGAT GACAAGGAAG AGAGAATAAG TATCCGTGGT 
GGGAAGCTGA TGATCTCTAA TACTAGGAAA AGCGATGCTG GCATGTACAC CTGTGTGGGA 
ACCAATATGG TGGGAGAAAG GGACAGCGAC CCTGCAGAGC TCACTGTCTT TGAACGACCC 
ACATTTCTCA GGAGGCCAAT TAACCAGGTG GTGCTAGAGG AAGAAGCTGT AGAATTCCGT 
TGTCAGGTCC AAGGAGATCC CCAGCCAACG GTGAGGTGGA AAAAAGATGA TGCAGACTTG 
CCGAGAGGAA GGTATGATAT CAAAGATGAC TACACGCTGA GAATTAAAAA GGCCATGAGT 
ACTGATGAAG GTACCTATGT GTGTATTGCT GAGAATCGGG TGGGAAAAGT GGAAGCCTCT 
GCTACCCTCA CTGTCCGAGT TCGCCCTGTT GCTCCTCCAC AGTTTGTGGT TAGGCCAAGA 
GATCAGATCG TTGCTCAAGG CCGAACAGTG ACATTCCCCT GTGAAACTAA AGGAAACCCA 
CAGCCAGCTG TTTTTTGGCA GAAAGAAGGC AGCCAGAACC TACTTTTCCC GAATCAACCT 
CAGCAGCCCA ACAGCCGATG TTCAGTGTCG CCCACGGGGG ACCTCACCAT CACCAACATC 
CAGCGTTCAG ATGCGGGTTA CTACATCTGC CAGGCCCTAA CCGTGGCAGG AAGCATTTTA 
GCTAAAGCAC AGTTGGAAGT TACTGACGTT TTGACAGATA GACCTCCACC CATAATCTTG 
CAAGGACCAA TAAACCAAAC ACTTGCAGTA GACGGTACAG CATTGTTGAA GTGTAAAGCC 
ACTGGTGAGC CTCTGCCTGT AATTAGCTGG CTAAAGGAGG GCTTTACTTT TCTGGGGAGA 
GATCCAAGAG CCACGATCCA AGACCAAGGA ACACTGCAGA TTAAGAATTT ACGGATATCT 
GATACTGGCA CTTATACTTG TGTGGCTACA AGTTCCAGTG GAGAGACTTC CTGGAGTGCA 
GTGCTGGATG TAACAGAATC TGGAGCAACA ATCAGTAAAA ATTATGATAT GAATGACCTC 
CCGGGACCAC CATCCAAACC TCAGGTCACT GATGTTTCTA AGAACAGTGT CACCTTATCC 
TGGCAGCCAG GTACACCTGG CGTTCTTCCT GCAAGCGCGT ATATCATTGA GGCTTTCAGC 
CAATCGGTGA GCAATAGCTG GCAGACAGTG GCAAACCATG TTAAGACAAC TCTGTATACA 
GTAAGGGGGC TGAGGCCCAA CACAATCTAC TTGTTTATGG TCAGAGCGAT CAACCCACAA 
GGTCTCAGTG ATCCAAGTCC TATGTCGGAT CCTGTACGCA CACAAGATAT CAGCCCCCCA 
GCACAAGGAG TGGACCACAG ACAGGTGCAG AAGGAATTAG GTGATGTCGT TGTTCGTCTC 
CATAATCCAG TTGTCCTGAC ACCTACAACT GTTCAAGTCA CATGGACGGT GGACCGACAA 
CCCCAGTTTA TTCAGGGCTA CAGAGTGATG TACCGTCAGA CTTCGGGACT ACAAGCCTCA 
ACTGTGTGGC AGAATCTAGA CGCCAAAGTC CCGACTGAGA GGAGTGCTGT CCTTGTGAAT 
TTGAAAAAGG GGGTGACTTA TGAAATTAAA GTCCGGCCGT ATTTTAACGA GTTCCAAGGA 
ATGGACAGTG AATCGAAAAC AGTCCGAACC ACTGAGGAAG CCCCAAGTGC CCCTCCCCAG 
TCTGTCACTG TGCTGACAGT TGGAAGTCAC AACAGCACAA GCATCAGTGT TTCCTGGGAT 
CCTCCACCAG CCGACCACCA GAATGGAATT ATTCAGGAAT ATAAGATCTG GTGTCTGGGA 
AACGAAACGC GATTCCATAT CAATAAAACG GTGGATGCAG CCATTCGCTC TGTAGTAATA 
GGTGGCTTGT TCCCTGGAAT TCAGTACCGG GTAGAAGTGG CAGCTAGCAC AAGTGCAGGG 
GTTGGAGTAA AAAGTGAACC ACAGCCGATA ATAATTGGGG GACGTAACGA AGTTGTCATT 
ACTGAAAACA ATAACAGCAT CACTGAGCAA ATCACGGATG TCGTGAAGCA ACCGGCATTT 
ATAGCTGGCA TTGGTGGTGC CTGCTGGGTA ATTCTGATGG GTTTTAGCAT CTGGTTGTAC 
TGGAGAAGAA AGAAGAGAAA GGGACTCAGC AATTATGCTG TAACATTTCA AAGAGGAGAT 
GGAGGACTAA TGAGCAATGG GAGCCGTCCA GGTCTTCTAA ATGCTGGCGA TCCCAATTAC 
CCATGGCTTG CTGATTCTTG GCCAGCCACG AGTTTGCCAG TGAACAATAG CAATAGTGGC 
CCAAATGAAA TTGGAAATTT TGGGCGTGGA GATGTGCTGC CTCCGGTGCC AGGCCAAGGG 
GATAAAACAG CGACCATGCT CTCGGATGGA GCCATTTATA GCAGCATTGA CTTCACTACC 
AAAACCACTT ACAACAGTTC CAGCCAAATA ACACAGGCCA CCCCATATGC CACTACACAA 
ATCCTGCATT CAAACAGCAT CCACGAACTG GCAGTTGATC TTCCTGATCC ACAGTGGAAA 
AGCTCAGTTC AACAGAAGAC AGACCTCATG GGATTTGGTT ATTCGCTACC TGATCAGAAC 
AAGGGGAACA ACGGTGGGAA AGGTGGAAAA AAGAAGAAAA CTAAAAATTC TTCGAAAGCG 
CAGAAAAACA ACGGATCCAC TTGGGCTAAT GTCCCTCTAC CTCCTCCTCC CGTCCAGCCC 
CTTCCTGGTA CAGAGCTGGG CCACTATGCT GCGGAACAAG AAAATGGCTA TGACAGTGAC 
AGCTGGTGCC CACCATTACC GGTGCAAACA TACTTGCATC AGGGTATGGA AGATGAGCTG 
GAAGAAGACG AAGATCGGGT CCCAACACCT CCTGTTCGCG GCGTGGCCTC TTCACCAGCT 
ATCTCTTTTG GACAGCAGTC CACTGCCACT CTTACTCCAT CCCCACGGGA AGAGATGCAA 
CCCATGCTGC AAGCTCACTT GGATGAGTTG ACAAGGGCCT ATCAGTTTGA TATAGCAAAA 
CAAACATGGC ACATTCAAAG CAATACCCCA CCTCCACAAC CCCCAGCTCC GCCATTAGGT 
TACGTGTCCG GAGCCTTGAT TTCTGATTTG GAGACAGATG TTCCAGATGA GGATGCTGAT 
GATGAAGAGG AACCATTAGA AATTCCCAGG CCCCTCAGAG CACTAGACCA GACACCTGGA 
TCCAGTATGG ACAATCTAGA CAGCTCTGTC ACAGGAAAAG CCTTTAGCTC CTCTCAAAGG 
CAGCGGCCCA CCAGCCCATT TTCTACGGAC AGTAACACCA GTGCTGCCCA GAATCAAAGC 
CAGAGGCCTC GGCCCACGAA AAAACATAAG GGAGGACGGA TGGACCCACA GCCAGTGTTA 
CCTCATCGGA GGGAAGGGAT GCCAGATGAG GAGTCCCTGG TGCCCTACAG CAAGCCCAGC 
TTCCCGTCTC CTGGCGGGCA CAGCTCATCA GGAACATCTT CCTCTAAAGG GTCCACTGGC 
CCTCGGAAGG CTGACGTGCT GAGAGGCAGC CACCAGAGGA ATGCCAATGA CCTTCTCGAC 
ATTGGATATG TGGGCTCAAA TAGTCAAGGA CAGTTTACAG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172506.1
CDS	241..4425
Translation	MARRQESICGRPWTWTPGLLMLIILGIHQGSGQGQGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKAMSTDEGTYVCIAENRVGKVEASATLTVRVRPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPINQTLAVDGTALLKCKATGEPLPVISWLKEGFTFLGRDPRATIQDQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDMNDLPGPPSKPQVTDVSKNSVTLSWQPGTPGVLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVVVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQASTVWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSHNSTSISVSWDPPPADHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVVIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGGRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPNYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTTYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSVQQKTDLMGFGYSLPDQNKGNNGGKGGKKKKTKNSSKAQKNNGSTWANVPLPPPPVQPLPGTELGHYAAEQENGYDSDSWCPPLPVQTYLHQGMEDELEEDEDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNTPPPQPPAPPLGYVSGALISDLETDVPDEDADDEEEPLEIPRPLRALDQTPGSSMDNLDSSVTGKAFSSSQRQRPTSPFSTDSNTSAAQNQSQRPRPTKKHKGGRMDPQPVLPHRREGMPDEESLVPYSKPSFPSPGGHSSSGTSSSKGSTGPRKADVLRGSHQRNANDLLDIGYVGSNSQGQFTE

Target ORF information:

RefSeq Version	XM_017317017.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317017.1

ORF Insert Sequence:

ATGGCCAGAA GACAAGAAAG TATCTGTGGA AGGCCGTGGA CGTGGACGCC AGGACTACTG 
ATGCTGATCA TCCTTGGCAT TCATCAGGGG AGTGGGCAAG GTCAAGGATC TCGTCTTCGT 
CAAGAAGACT TTCCCCCCAG GATCGTAGAA CACCCTTCTG ATGTCATCGT CTCCAAGGGA 
GAGCCCACCA CTCTGAACTG TAAAGCAGAG GGCCGACCCA CCCCCACCAT TGAATGGTAC 
AAGGATGGTG AGAGGGTGGA GACAGACAAG GATGATCCCA GGTCCCACAG AATGCTTCTG 
CCCAGCGGAT CTTTATTCTT TTTGCGAATT GTTCATGGGC GCAGAAGTAA ACCGGACGAA 
GGGAGTTACG TTTGTGTTGC AAGGAACTAT CTTGGTGAAG CAGTGAGTCG AAATGCATCT 
CTGGAAGTGG CATTATTGCG AGATGACTTC CGGCAAAACC CCACAGATGT GGTAGTCGCA 
GCTGGAGAGC CTGCAATCTT GGAGTGCCAG CCACCACGGG GACACCCAGA ACCAACCATC 
TACTGGAAAA AGGACAAAGT CCGAATTGAT GACAAGGAAG AGAGAATAAG TATCCGTGGT 
GGGAAGCTGA TGATCTCTAA TACTAGGAAA AGCGATGCTG GCATGTACAC CTGTGTGGGA 
ACCAATATGG TGGGAGAAAG GGACAGCGAC CCTGCAGAGC TCACTGTCTT TGAACGACCC 
ACATTTCTCA GGAGGCCAAT TAACCAGGTG GTGCTAGAGG AAGAAGCTGT AGAATTCCGT 
TGTCAGGTCC AAGGAGATCC CCAGCCAACG GTGAGGTGGA AAAAAGATGA TGCAGACTTG 
CCGAGAGGAA GGTATGATAT CAAAGATGAC TACACGCTGA GAATTAAAAA GGCCATGAGT 
ACTGATGAAG GTACCTATGT GTGTATTGCT GAGAATCGGG TGGGAAAAGT GGAAGCCTCT 
GCTACCCTCA CTGTCCGAGT TCGCCCTGTT GCTCCTCCAC AGTTTGTGGT TAGGCCAAGA 
GATCAGATCG TTGCTCAAGG CCGAACAGTG ACATTCCCCT GTGAAACTAA AGGAAACCCA 
CAGCCAGCTG TTTTTTGGCA GAAAGAAGGC AGCCAGAACC TACTTTTCCC GAATCAACCT 
CAGCAGCCCA ACAGCCGATG TTCAGTGTCG CCCACGGGGG ACCTCACCAT CACCAACATC 
CAGCGTTCAG ATGCGGGTTA CTACATCTGC CAGGCCCTAA CCGTGGCAGG AAGCATTTTA 
GCTAAAGCAC AGTTGGAAGT TACTGACGTT TTGACAGATA GACCTCCACC CATAATCTTG 
CAAGGACCAA TAAACCAAAC ACTTGCAGTA GACGGTACAG CATTGTTGAA GTGTAAAGCC 
ACTGGTGAGC CTCTGCCTGT AATTAGCTGG CTAAAGGAGG GCTTTACTTT TCTGGGGAGA 
GATCCAAGAG CCACGATCCA AGACCAAGGA ACACTGCAGA TTAAGAATTT ACGGATATCT 
GATACTGGCA CTTATACTTG TGTGGCTACA AGTTCCAGTG GAGAGACTTC CTGGAGTGCA 
GTGCTGGATG TAACAGAATC TGGAGCAACA ATCAGTAAAA ATTATGATAT GAATGACCTC 
CCGGGACCAC CATCCAAACC TCAGGTCACT GATGTTTCTA AGAACAGTGT CACCTTATCC 
TGGCAGCCAG GTACACCTGG CGTTCTTCCT GCAAGCGCGT ATATCATTGA GGCTTTCAGC 
CAATCGGTGA GCAATAGCTG GCAGACAGTG GCAAACCATG TTAAGACAAC TCTGTATACA 
GTAAGGGGGC TGAGGCCCAA CACAATCTAC TTGTTTATGG TCAGAGCGAT CAACCCACAA 
GGTCTCAGTG ATCCAAGTCC TATGTCGGAT CCTGTACGCA CACAAGATAT CAGCCCCCCA 
GCACAAGGAG TGGACCACAG ACAGGTGCAG AAGGAATTAG GTGATGTCGT TGTTCGTCTC 
CATAATCCAG TTGTCCTGAC ACCTACAACT GTTCAAGTCA CATGGACGGT GGACCGACAA 
CCCCAGTTTA TTCAGGGCTA CAGAGTGATG TACCGTCAGA CTTCGGGACT ACAAGCCTCA 
ACTGTGTGGC AGAATCTAGA CGCCAAAGTC CCGACTGAGA GGAGTGCTGT CCTTGTGAAT 
TTGAAAAAGG GGGTGACTTA TGAAATTAAA GTCCGGCCGT ATTTTAACGA GTTCCAAGGA 
ATGGACAGTG AATCGAAAAC AGTCCGAACC ACTGAGGAAG CCCCAAGTGC CCCTCCCCAG 
TCTGTCACTG TGCTGACAGT TGGAAGTCAC AACAGCACAA GCATCAGTGT TTCCTGGGAT 
CCTCCACCAG CCGACCACCA GAATGGAATT ATTCAGGAAT ATAAGATCTG GTGTCTGGGA 
AACGAAACGC GATTCCATAT CAATAAAACG GTGGATGCAG CCATTCGCTC TGTAGTAATA 
GGTGGCTTGT TCCCTGGAAT TCAGTACCGG GTAGAAGTGG CAGCTAGCAC AAGTGCAGGG 
GTTGGAGTAA AAAGTGAACC ACAGCCGATA ATAATTGGGG GACGTAACGA AGTTGTCATT 
ACTGAAAACA ATAACAGCAT CACTGAGCAA ATCACGGATG TCGTGAAGCA ACCGGCATTT 
ATAGCTGGCA TTGGTGGTGC CTGCTGGGTA ATTCTGATGG GTTTTAGCAT CTGGTTGTAC 
TGGAGAAGAA AGAAGAGAAA GGGACTCAGC AATTATGCTG TAACATTTCA AAGAGGAGAT 
GGAGGACTAA TGAGCAATGG GAGCCGTCCA GGTCTTCTAA ATGCTGGCGA TCCCAATTAC 
CCATGGCTTG CTGATTCTTG GCCAGCCACG AGTTTGCCAG TGAACAATAG CAATAGTGGC 
CCAAATGAAA TTGGAAATTT TGGGCGTGGA GATGTGCTGC CTCCGGTGCC AGGCCAAGGG 
GATAAAACAG CGACCATGCT CTCGGATGGA GCCATTTATA GCAGCATTGA CTTCACTACC 
AAAACCACTT ACAACAGTTC CAGCCAAATA ACACAGGCCA CCCCATATGC CACTACACAA 
ATCCTGCATT CAAACAGCAT CCACGAACTG GCAGTTGATC TTCCTGATCC ACAGTGGAAA 
AGCTCAGTTC AACAGAAGAC AGACCTCATG GGATTTGGTT ATTCGCTACC TGATCAGAAC 
AAGGGGAACA ACGGTGGGAA AGGTGGAAAA AAGAAGAAAA CTAAAAATTC TTCGAAAGCG 
CAGAAAAACA ACGGATCCAC TTGGGCTAAT GTCCCTCTAC CTCCTCCTCC CGTCCAGCCC 
CTTCCTGGTA CAGAGCTGGG CCACTATGCT GCGGAACAAG AAAATGGCTA TGACAGTGAC 
AGCTGGTGCC CACCATTACC GGTGCAAACA TACTTGCATC AGGGTATGGA AGATGAGCTG 
GAAGAAGACG AAGATCGGGT CCCAACACCT CCTGTTCGCG GCGTGGCCTC TTCACCAGCT 
ATCTCTTTTG GACAGCAGTC CACTGCCACT CTTACTCCAT CCCCACGGGA AGAGATGCAA 
CCCATGCTGC AAGCTCACTT GGATGAGTTG ACAAGGGCCT ATCAGTTTGA TATAGCAAAA 
CAAACATGGC ACATTCAAAG CAATACCCCA CCTCCACAAC CCCCAGCTCC GCCATTAGGT 
TACGTGTCCG GAGCCTTGAT TTCTGATTTG GAGACAGATG TTCCAGATGA GGATGCTGAT 
GATGAAGAGG AACCATTAGA AATTCCCAGG CCCCTCAGAG CACTAGACCA GACACCTGGA 
TCCAGTATGG ACAATCTAGA CAGCTCTGTC ACAGGAAAAG CCTTTAGCTC CTCTCAAAGG 
CAGCGGCCCA CCAGCCCATT TTCTACGGAC AGTAACACCA GTGCTGCCCA GAATCAAAGC 
CAGAGGCCTC GGCCCACGAA AAAACATAAG GGAGGACGGA TGGACCCACA GCCAGTGTTA 
CCTCATCGGA GGGAAGGGAT GCCAGATGAG GAGTCCCTGG TGCCCTACAG CAAGCCCAGC 
TTCCCGTCTC CTGGCGGGCA CAGCTCATCA GGAACATCTT CCTCTAAAGG GTCCACTGGC 
CCTCGGAAGG CTGACGTGCT GAGAGGCAGC CACCAGAGGA ATGCCAATGA CCTTCTCGAC 
ATTGGATATG TGGGCTCAAA TAGTCAAGGA CAGTTTACAG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo2 ( XM_017317018.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172507.1 Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X22, mRNA.

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CloneID	OMu79345
Clone ID Related Accession (Same CDS sequence)	NM_001358494.1 , XM_017317018.1
Accession Version	XM_017317018.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4140bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	roundabout homolog 2 isoform X22
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCCTC TGATGTTTAC ACTATTATTG CTCTTTGGAT TTCTCTGCAT TCAGATTGAT 
GGATCTCGTC TTCGTCAAGA AGACTTTCCC CCCAGGATCG TAGAACACCC TTCTGATGTC 
ATCGTCTCCA AGGGAGAGCC CACCACTCTG AACTGTAAAG CAGAGGGCCG ACCCACCCCC 
ACCATTGAAT GGTACAAGGA TGGTGAGAGG GTGGAGACAG ACAAGGATGA TCCCAGGTCC 
CACAGAATGC TTCTGCCCAG CGGATCTTTA TTCTTTTTGC GAATTGTTCA TGGGCGCAGA 
AGTAAACCGG ACGAAGGGAG TTACGTTTGT GTTGCAAGGA ACTATCTTGG TGAAGCAGTG 
AGTCGAAATG CATCTCTGGA AGTGGCATTA TTGCGAGATG ACTTCCGGCA AAACCCCACA 
GATGTGGTAG TCGCAGCTGG AGAGCCTGCA ATCTTGGAGT GCCAGCCACC ACGGGGACAC 
CCAGAACCAA CCATCTACTG GAAAAAGGAC AAAGTCCGAA TTGATGACAA GGAAGAGAGA 
ATAAGTATCC GTGGTGGGAA GCTGATGATC TCTAATACTA GGAAAAGCGA TGCTGGCATG 
TACACCTGTG TGGGAACCAA TATGGTGGGA GAAAGGGACA GCGACCCTGC AGAGCTCACT 
GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTGCT AGAGGAAGAA 
GCTGTAGAAT TCCGTTGTCA GGTCCAAGGA GATCCCCAGC CAACGGTGAG GTGGAAAAAA 
GATGATGCAG ACTTGCCGAG AGGAAGGTAT GATATCAAAG ATGACTACAC GCTGAGAATT 
AAAAAGGCCA TGAGTACTGA TGAAGGTACC TATGTGTGTA TTGCTGAGAA TCGGGTGGGA 
AAAGTGGAAG CCTCTGCTAC CCTCACTGTC CGAGTTCGCC CTGTTGCTCC TCCACAGTTT 
GTGGTTAGGC CAAGAGATCA GATCGTTGCT CAAGGCCGAA CAGTGACATT CCCCTGTGAA 
ACTAAAGGAA ACCCACAGCC AGCTGTTTTT TGGCAGAAAG AAGGCAGCCA GAACCTACTT 
TTCCCGAATC AACCTCAGCA GCCCAACAGC CGATGTTCAG TGTCGCCCAC GGGGGACCTC 
ACCATCACCA ACATCCAGCG TTCAGATGCG GGTTACTACA TCTGCCAGGC CCTAACCGTG 
GCAGGAAGCA TTTTAGCTAA AGCACAGTTG GAAGTTACTG ACGTTTTGAC AGATAGACCT 
CCACCCATAA TCTTGCAAGG ACCAATAAAC CAAACACTTG CAGTAGACGG TACAGCATTG 
TTGAAGTGTA AAGCCACTGG TGAGCCTCTG CCTGTAATTA GCTGGCTAAA GGAGGGCTTT 
ACTTTTCTGG GGAGAGATCC AAGAGCCACG ATCCAAGACC AAGGAACACT GCAGATTAAG 
AATTTACGGA TATCTGATAC TGGCACTTAT ACTTGTGTGG CTACAAGTTC CAGTGGAGAG 
ACTTCCTGGA GTGCAGTGCT GGATGTAACA GAATCTGGAG CAACAATCAG TAAAAATTAT 
GATATGAATG ACCTCCCGGG ACCACCATCC AAACCTCAGG TCACTGATGT TTCTAAGAAC 
AGTGTCACCT TATCCTGGCA GCCAGGTACA CCTGGCGTTC TTCCTGCAAG CGCGTATATC 
ATTGAGGCTT TCAGCCAATC GGTGAGCAAT AGCTGGCAGA CAGTGGCAAA CCATGTTAAG 
ACAACTCTGT ATACAGTAAG GGGGCTGAGG CCCAACACAA TCTACTTGTT TATGGTCAGA 
GCGATCAACC CACAAGGTCT CAGTGATCCA AGTCCTATGT CGGATCCTGT ACGCACACAA 
GATATCAGCC CCCCAGCACA AGGAGTGGAC CACAGACAGG TGCAGAAGGA ATTAGGTGAT 
GTCGTTGTTC GTCTCCATAA TCCAGTTGTC CTGACACCTA CAACTGTTCA AGTCACATGG 
ACGGTGGACC GACAACCCCA GTTTATTCAG GGCTACAGAG TGATGTACCG TCAGACTTCG 
GGACTACAAG CCTCAACTGT GTGGCAGAAT CTAGACGCCA AAGTCCCGAC TGAGAGGAGT 
GCTGTCCTTG TGAATTTGAA AAAGGGGGTG ACTTATGAAA TTAAAGTCCG GCCGTATTTT 
AACGAGTTCC AAGGAATGGA CAGTGAATCG AAAACAGTCC GAACCACTGA GGAAGCCCCA 
AGTGCCCCTC CCCAGTCTGT CACTGTGCTG ACAGTTGGAA GTCACAACAG CACAAGCATC 
AGTGTTTCCT GGGATCCTCC ACCAGCCGAC CACCAGAATG GAATTATTCA GGAATATAAG 
ATCTGGTGTC TGGGAAACGA AACGCGATTC CATATCAATA AAACGGTGGA TGCAGCCATT 
CGCTCTGTAG TAATAGGTGG CTTGTTCCCT GGAATTCAGT ACCGGGTAGA AGTGGCAGCT 
AGCACAAGTG CAGGGGTTGG AGTAAAAAGT GAACCACAGC CGATAATAAT TGGGGGACGT 
AACGAAGTTG TCATTACTGA AAACAATAAC AGCATCACTG AGCAAATCAC GGATGTCGTG 
AAGCAACCGG CATTTATAGC TGGCATTGGT GGTGCCTGCT GGGTAATTCT GATGGGTTTT 
AGCATCTGGT TGTACTGGAG AAGAAAGAAG AGAAAGGGAC TCAGCAATTA TGCTGTAACA 
TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGGAGCC GTCCAGGTCT TCTAAATGCT 
GGCGATCCCA ATTACCCATG GCTTGCTGAT TCTTGGCCAG CCACGAGTTT GCCAGTGAAC 
AATAGCAATA GTGGCCCAAA TGAAATTGGA AATTTTGGGC GTGGAGATGT GCTGCCTCCG 
GTGCCAGGCC AAGGGGATAA AACAGCGACC ATGCTCTCGG ATGGAGCCAT TTATAGCAGC 
ATTGACTTCA CTACCAAAAC CACTTACAAC AGTTCCAGCC AAATAACACA GGCCACCCCA 
TATGCCACTA CACAAATCCT GCATTCAAAC AGCATCCACG AACTGGCAGT TGATCTTCCT 
GATCCACAGT GGAAAAGCTC AGTTCAACAG AAGACAGACC TCATGGGATT TGGTTATTCG 
CTACCTGATC AGAACAAGGG GAACAACGGT GGGAAAGGTG GAAAAAAGAA GAAAACTAAA 
AATTCTTCGA AAGCGCAGAA AAACAACGGA TCCACTTGGG CTAATGTCCC TCTACCTCCT 
CCTCCCGTCC AGCCCCTTCC TGGTACAGAG CTGGGCCACT ATGCTGCGGA ACAAGAAAAT 
GGCTATGACA GTGACAGCTG GTGCCCACCA TTACCGGTGC AAACATACTT GCATCAGGGT 
ATGGAAGATG AGCTGGAAGA AGACGAAGAT CGGGTCCCAA CACCTCCTGT TCGCGGCGTG 
GCCTCTTCAC CAGCTATCTC TTTTGGACAG CAGTCCACTG CCACTCTTAC TCCATCCCCA 
CGGGAAGAGA TGCAACCCAT GCTGCAAGCT CACTTGGATG AGTTGACAAG GGCCTATCAG 
TTTGATATAG CAAAACAAAC ATGGCACATT CAAAGCAATA CCCCACCTCC ACAACCCCCA 
GCTCCGCCAT TAGGTTACGT GTCCGGAGCC TTGATTTCTG ATTTGGAGAC AGATGTTCCA 
GATGAGGATG CTGATGATGA AGAGGAACCA TTAGAAATTC CCAGGCCCCT CAGAGCACTA 
GACCAGACAC CTGGATCCAG TATGGACAAT CTAGACAGCT CTGTCACAGG AAAAGCCTTT 
AGCTCCTCTC AAAGGCAGCG GCCCACCAGC CCATTTTCTA CGGACAGTAA CACCAGTGCT 
GCCCAGAATC AAAGCCAGAG GCCTCGGCCC ACGAAAAAAC ATAAGGGAGG ACGGATGGAC 
CCACAGCCAG TGTTACCTCA TCGGAGGGAA GGGATGCCAG ATGAGGAGTC CCTGGTGCCC 
TACAGCAAGC CCAGCTTCCC GTCTCCTGGC GGGCACAGCT CATCAGGAAC ATCTTCCTCT 
AAAGGGTCCA CTGGCCCTCG GAAGGCTGAC GTGCTGAGAG GCAGCCACCA GAGGAATGCC 
AATGACCTTC TCGACATTGG ATATGTGGGC TCAAATAGTC AAGGACAGTT TACAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172507.1
CDS	874..5013
Translation	MNPLMFTLLLLFGFLCIQIDGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKAMSTDEGTYVCIAENRVGKVEASATLTVRVRPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPINQTLAVDGTALLKCKATGEPLPVISWLKEGFTFLGRDPRATIQDQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDMNDLPGPPSKPQVTDVSKNSVTLSWQPGTPGVLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVVVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQASTVWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSHNSTSISVSWDPPPADHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVVIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGGRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPNYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTTYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSVQQKTDLMGFGYSLPDQNKGNNGGKGGKKKKTKNSSKAQKNNGSTWANVPLPPPPVQPLPGTELGHYAAEQENGYDSDSWCPPLPVQTYLHQGMEDELEEDEDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNTPPPQPPAPPLGYVSGALISDLETDVPDEDADDEEEPLEIPRPLRALDQTPGSSMDNLDSSVTGKAFSSSQRQRPTSPFSTDSNTSAAQNQSQRPRPTKKHKGGRMDPQPVLPHRREGMPDEESLVPYSKPSFPSPGGHSSSGTSSSKGSTGPRKADVLRGSHQRNANDLLDIGYVGSNSQGQFTE

Target ORF information:

RefSeq Version	XM_017317018.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317018.1

ORF Insert Sequence:

ATGAATCCTC TGATGTTTAC ACTATTATTG CTCTTTGGAT TTCTCTGCAT TCAGATTGAT 
GGATCTCGTC TTCGTCAAGA AGACTTTCCC CCCAGGATCG TAGAACACCC TTCTGATGTC 
ATCGTCTCCA AGGGAGAGCC CACCACTCTG AACTGTAAAG CAGAGGGCCG ACCCACCCCC 
ACCATTGAAT GGTACAAGGA TGGTGAGAGG GTGGAGACAG ACAAGGATGA TCCCAGGTCC 
CACAGAATGC TTCTGCCCAG CGGATCTTTA TTCTTTTTGC GAATTGTTCA TGGGCGCAGA 
AGTAAACCGG ACGAAGGGAG TTACGTTTGT GTTGCAAGGA ACTATCTTGG TGAAGCAGTG 
AGTCGAAATG CATCTCTGGA AGTGGCATTA TTGCGAGATG ACTTCCGGCA AAACCCCACA 
GATGTGGTAG TCGCAGCTGG AGAGCCTGCA ATCTTGGAGT GCCAGCCACC ACGGGGACAC 
CCAGAACCAA CCATCTACTG GAAAAAGGAC AAAGTCCGAA TTGATGACAA GGAAGAGAGA 
ATAAGTATCC GTGGTGGGAA GCTGATGATC TCTAATACTA GGAAAAGCGA TGCTGGCATG 
TACACCTGTG TGGGAACCAA TATGGTGGGA GAAAGGGACA GCGACCCTGC AGAGCTCACT 
GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTGCT AGAGGAAGAA 
GCTGTAGAAT TCCGTTGTCA GGTCCAAGGA GATCCCCAGC CAACGGTGAG GTGGAAAAAA 
GATGATGCAG ACTTGCCGAG AGGAAGGTAT GATATCAAAG ATGACTACAC GCTGAGAATT 
AAAAAGGCCA TGAGTACTGA TGAAGGTACC TATGTGTGTA TTGCTGAGAA TCGGGTGGGA 
AAAGTGGAAG CCTCTGCTAC CCTCACTGTC CGAGTTCGCC CTGTTGCTCC TCCACAGTTT 
GTGGTTAGGC CAAGAGATCA GATCGTTGCT CAAGGCCGAA CAGTGACATT CCCCTGTGAA 
ACTAAAGGAA ACCCACAGCC AGCTGTTTTT TGGCAGAAAG AAGGCAGCCA GAACCTACTT 
TTCCCGAATC AACCTCAGCA GCCCAACAGC CGATGTTCAG TGTCGCCCAC GGGGGACCTC 
ACCATCACCA ACATCCAGCG TTCAGATGCG GGTTACTACA TCTGCCAGGC CCTAACCGTG 
GCAGGAAGCA TTTTAGCTAA AGCACAGTTG GAAGTTACTG ACGTTTTGAC AGATAGACCT 
CCACCCATAA TCTTGCAAGG ACCAATAAAC CAAACACTTG CAGTAGACGG TACAGCATTG 
TTGAAGTGTA AAGCCACTGG TGAGCCTCTG CCTGTAATTA GCTGGCTAAA GGAGGGCTTT 
ACTTTTCTGG GGAGAGATCC AAGAGCCACG ATCCAAGACC AAGGAACACT GCAGATTAAG 
AATTTACGGA TATCTGATAC TGGCACTTAT ACTTGTGTGG CTACAAGTTC CAGTGGAGAG 
ACTTCCTGGA GTGCAGTGCT GGATGTAACA GAATCTGGAG CAACAATCAG TAAAAATTAT 
GATATGAATG ACCTCCCGGG ACCACCATCC AAACCTCAGG TCACTGATGT TTCTAAGAAC 
AGTGTCACCT TATCCTGGCA GCCAGGTACA CCTGGCGTTC TTCCTGCAAG CGCGTATATC 
ATTGAGGCTT TCAGCCAATC GGTGAGCAAT AGCTGGCAGA CAGTGGCAAA CCATGTTAAG 
ACAACTCTGT ATACAGTAAG GGGGCTGAGG CCCAACACAA TCTACTTGTT TATGGTCAGA 
GCGATCAACC CACAAGGTCT CAGTGATCCA AGTCCTATGT CGGATCCTGT ACGCACACAA 
GATATCAGCC CCCCAGCACA AGGAGTGGAC CACAGACAGG TGCAGAAGGA ATTAGGTGAT 
GTCGTTGTTC GTCTCCATAA TCCAGTTGTC CTGACACCTA CAACTGTTCA AGTCACATGG 
ACGGTGGACC GACAACCCCA GTTTATTCAG GGCTACAGAG TGATGTACCG TCAGACTTCG 
GGACTACAAG CCTCAACTGT GTGGCAGAAT CTAGACGCCA AAGTCCCGAC TGAGAGGAGT 
GCTGTCCTTG TGAATTTGAA AAAGGGGGTG ACTTATGAAA TTAAAGTCCG GCCGTATTTT 
AACGAGTTCC AAGGAATGGA CAGTGAATCG AAAACAGTCC GAACCACTGA GGAAGCCCCA 
AGTGCCCCTC CCCAGTCTGT CACTGTGCTG ACAGTTGGAA GTCACAACAG CACAAGCATC 
AGTGTTTCCT GGGATCCTCC ACCAGCCGAC CACCAGAATG GAATTATTCA GGAATATAAG 
ATCTGGTGTC TGGGAAACGA AACGCGATTC CATATCAATA AAACGGTGGA TGCAGCCATT 
CGCTCTGTAG TAATAGGTGG CTTGTTCCCT GGAATTCAGT ACCGGGTAGA AGTGGCAGCT 
AGCACAAGTG CAGGGGTTGG AGTAAAAAGT GAACCACAGC CGATAATAAT TGGGGGACGT 
AACGAAGTTG TCATTACTGA AAACAATAAC AGCATCACTG AGCAAATCAC GGATGTCGTG 
AAGCAACCGG CATTTATAGC TGGCATTGGT GGTGCCTGCT GGGTAATTCT GATGGGTTTT 
AGCATCTGGT TGTACTGGAG AAGAAAGAAG AGAAAGGGAC TCAGCAATTA TGCTGTAACA 
TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGGAGCC GTCCAGGTCT TCTAAATGCT 
GGCGATCCCA ATTACCCATG GCTTGCTGAT TCTTGGCCAG CCACGAGTTT GCCAGTGAAC 
AATAGCAATA GTGGCCCAAA TGAAATTGGA AATTTTGGGC GTGGAGATGT GCTGCCTCCG 
GTGCCAGGCC AAGGGGATAA AACAGCGACC ATGCTCTCGG ATGGAGCCAT TTATAGCAGC 
ATTGACTTCA CTACCAAAAC CACTTACAAC AGTTCCAGCC AAATAACACA GGCCACCCCA 
TATGCCACTA CACAAATCCT GCATTCAAAC AGCATCCACG AACTGGCAGT TGATCTTCCT 
GATCCACAGT GGAAAAGCTC AGTTCAACAG AAGACAGACC TCATGGGATT TGGTTATTCG 
CTACCTGATC AGAACAAGGG GAACAACGGT GGGAAAGGTG GAAAAAAGAA GAAAACTAAA 
AATTCTTCGA AAGCGCAGAA AAACAACGGA TCCACTTGGG CTAATGTCCC TCTACCTCCT 
CCTCCCGTCC AGCCCCTTCC TGGTACAGAG CTGGGCCACT ATGCTGCGGA ACAAGAAAAT 
GGCTATGACA GTGACAGCTG GTGCCCACCA TTACCGGTGC AAACATACTT GCATCAGGGT 
ATGGAAGATG AGCTGGAAGA AGACGAAGAT CGGGTCCCAA CACCTCCTGT TCGCGGCGTG 
GCCTCTTCAC CAGCTATCTC TTTTGGACAG CAGTCCACTG CCACTCTTAC TCCATCCCCA 
CGGGAAGAGA TGCAACCCAT GCTGCAAGCT CACTTGGATG AGTTGACAAG GGCCTATCAG 
TTTGATATAG CAAAACAAAC ATGGCACATT CAAAGCAATA CCCCACCTCC ACAACCCCCA 
GCTCCGCCAT TAGGTTACGT GTCCGGAGCC TTGATTTCTG ATTTGGAGAC AGATGTTCCA 
GATGAGGATG CTGATGATGA AGAGGAACCA TTAGAAATTC CCAGGCCCCT CAGAGCACTA 
GACCAGACAC CTGGATCCAG TATGGACAAT CTAGACAGCT CTGTCACAGG AAAAGCCTTT 
AGCTCCTCTC AAAGGCAGCG GCCCACCAGC CCATTTTCTA CGGACAGTAA CACCAGTGCT 
GCCCAGAATC AAAGCCAGAG GCCTCGGCCC ACGAAAAAAC ATAAGGGAGG ACGGATGGAC 
CCACAGCCAG TGTTACCTCA TCGGAGGGAA GGGATGCCAG ATGAGGAGTC CCTGGTGCCC 
TACAGCAAGC CCAGCTTCCC GTCTCCTGGC GGGCACAGCT CATCAGGAAC ATCTTCCTCT 
AAAGGGTCCA CTGGCCCTCG GAAGGCTGAC GTGCTGAGAG GCAGCCACCA GAGGAATGCC 
AATGACCTTC TCGACATTGG ATATGTGGGC TCAAATAGTC AAGGACAGTT TACAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo2 ( NM_001358493.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001345422.1 Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant 1, mRNA.

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CloneID	OMu79341
Clone ID Related Accession (Same CDS sequence)	XM_017317013.1 , NM_001358493.1
Accession Version	NM_001358493.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4608bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	roundabout homolog 2 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC115120.4, AC158234.2, AC112155.3, AC134452.4, AC122049.2 and AC162374.4. On Dec 1, 2017 this sequence version replaced XM_017317013.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END##

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT 
CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG 
GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC 
CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA 
GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT 
ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG 
GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG 
CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT 
AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT 
TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC 
GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC 
CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT 
ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA 
CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA 
TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA 
GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT 
CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT 
GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG 
CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG 
GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC 
ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG 
GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT 
GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA 
GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT 
GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG 
GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT 
GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT 
CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG 
CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG 
CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT 
TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG 
GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT 
GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT 
GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGCCT TACTTTACAT CCCTGACTAC 
CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC 
ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA 
AAGAAGAAAA CTAAAAATTC TTCGAAAGCG CAGAAAAACA ACGGATCCAC TTGGGCTAAT 
GTCCCTCTAC CTCCTCCTCC CGTCCAGCCC CTTCCTGGTA CAGAGCTGGG CCACTATGCT 
GCGGAACAAG AAAATGGCTA TGACAGTGAC AGCTGGTGCC CACCATTACC GGTGCAAACA 
TACTTGCATC AGGGTATGGA AGATGAGCTG GAAGAAGACG AAGATCGGGT CCCAACACCT 
CCTGTTCGCG GCGTGGCCTC TTCACCAGCT ATCTCTTTTG GACAGCAGTC CACTGCCACT 
CTTACTCCAT CCCCACGGGA AGAGATGCAA CCCATGCTGC AAGCTCACTT GGATGAGTTG 
ACAAGGGCCT ATCAGTTTGA TATAGCAAAA CAAACATGGC ACATTCAAAG CAATACCCCA 
CCTCCACAAC CCCCAGCTCC GCCATTAGGT TACGTGTCCG GAGCCTTGAT TTCTGATTTG 
GAGACAGATG TTCCAGATGA GGATGCTGAT GATGAAGAGG AACCATTAGA AATTCCCAGG 
CCCCTCAGAG CACTAGACCA GACACCTGGA TCCAGTATGG ACAATCTAGA CAGCTCTGTC 
ACAGGAAAAG CCTTTAGCTC CTCTCAAAGG CAGCGGCCCA CCAGCCCATT TTCTACGGAC 
AGTAACACCA GTGCTGCCCA GAATCAAAGC CAGAGGCCTC GGCCCACGAA AAAACATAAG 
GGAGGACGGA TGGACCCACA GCCAGTGTTA CCTCATCGGA GGGAAGGGAT GCCAGATGAT 
CTTCCCCCAC CACCAGATCC CCCACCAGGT CAGGGTTTAA GGCAGCAGAT AGGCCTGAGC 
CAACACTCTG GTAACGTGGA AAACTCAACA GAGAGAAAAG GAAGCTCTCT AGAGAGACAA 
CAAGCAGCCA ACTTAGAAGA CACAAAGAGC TCATTGGATT GTCCAGCTAA AACTGTTCTA 
GAGTGGCAGC GTCAAACCCA GGACTGGATA AACTCTACAG AACGCCAAGA AGAAACTCGG 
AAAGCCCCAC ACAAACAAGG GGTTGGATCA GAGGAGTCCC TGGTGCCCTA CAGCAAGCCC 
AGCTTCCCGT CTCCTGGCGG GCACAGCTCA TCAGGAACAT CTTCCTCTAA AGGGTCCACT 
GGCCCTCGGA AGGCTGACGT GCTGAGAGGC AGCCACCAGA GGAATGCCAA TGACCTTCTC 
GACATTGGAT ATGTGGGCTC AAATAGTCAA GGACAGTTTA CAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001345422.1
CDS	353..4960
Translation	MLSSEKVDKITRLELLFVLMLFFSGPTLGKLTRTPWKGKPWKGGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKAMSTDEGTYVCIAENRVGKVEASATLTVRVRPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPINQTLAVDGTALLKCKATGEPLPVISWLKEGFTFLGRDPRATIQDQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDMNDLPGPPSKPQVTDVSKNSVTLSWQPGTPGVLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVVVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQASTVWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSHNSTSISVSWDPPPADHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVVIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGGRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPNYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTTYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSVQQKTDLMGFGYSLPDQNKGNNALLYIPDYRLAEGLSNRMPHNQSQDFSTTSSHNSSERSGSLSGGKGGKKKKTKNSSKAQKNNGSTWANVPLPPPPVQPLPGTELGHYAAEQENGYDSDSWCPPLPVQTYLHQGMEDELEEDEDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNTPPPQPPAPPLGYVSGALISDLETDVPDEDADDEEEPLEIPRPLRALDQTPGSSMDNLDSSVTGKAFSSSQRQRPTSPFSTDSNTSAAQNQSQRPRPTKKHKGGRMDPQPVLPHRREGMPDDLPPPPDPPPGQGLRQQIGLSQHSGNVENSTERKGSSLERQQAANLEDTKSSLDCPAKTVLEWQRQTQDWINSTERQEETRKAPHKQGVGSEESLVPYSKPSFPSPGGHSSSGTSSSKGSTGPRKADVLRGSHQRNANDLLDIGYVGSNSQGQFTE

Target ORF information:

RefSeq Version	NM_001358493.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001358493.1

ORF Insert Sequence:

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT 
CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG 
GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC 
CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA 
GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT 
ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG 
GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG 
CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT 
AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT 
TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC 
GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC 
CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT 
ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA 
CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA 
TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA 
GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT 
CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT 
GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG 
CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG 
GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC 
ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG 
GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT 
GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA 
GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT 
GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG 
GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT 
GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT 
CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG 
CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG 
CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT 
TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG 
GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT 
GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT 
GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGCCT TACTTTACAT CCCTGACTAC 
CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC 
ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA 
AAGAAGAAAA CTAAAAATTC TTCGAAAGCG CAGAAAAACA ACGGATCCAC TTGGGCTAAT 
GTCCCTCTAC CTCCTCCTCC CGTCCAGCCC CTTCCTGGTA CAGAGCTGGG CCACTATGCT 
GCGGAACAAG AAAATGGCTA TGACAGTGAC AGCTGGTGCC CACCATTACC GGTGCAAACA 
TACTTGCATC AGGGTATGGA AGATGAGCTG GAAGAAGACG AAGATCGGGT CCCAACACCT 
CCTGTTCGCG GCGTGGCCTC TTCACCAGCT ATCTCTTTTG GACAGCAGTC CACTGCCACT 
CTTACTCCAT CCCCACGGGA AGAGATGCAA CCCATGCTGC AAGCTCACTT GGATGAGTTG 
ACAAGGGCCT ATCAGTTTGA TATAGCAAAA CAAACATGGC ACATTCAAAG CAATACCCCA 
CCTCCACAAC CCCCAGCTCC GCCATTAGGT TACGTGTCCG GAGCCTTGAT TTCTGATTTG 
GAGACAGATG TTCCAGATGA GGATGCTGAT GATGAAGAGG AACCATTAGA AATTCCCAGG 
CCCCTCAGAG CACTAGACCA GACACCTGGA TCCAGTATGG ACAATCTAGA CAGCTCTGTC 
ACAGGAAAAG CCTTTAGCTC CTCTCAAAGG CAGCGGCCCA CCAGCCCATT TTCTACGGAC 
AGTAACACCA GTGCTGCCCA GAATCAAAGC CAGAGGCCTC GGCCCACGAA AAAACATAAG 
GGAGGACGGA TGGACCCACA GCCAGTGTTA CCTCATCGGA GGGAAGGGAT GCCAGATGAT 
CTTCCCCCAC CACCAGATCC CCCACCAGGT CAGGGTTTAA GGCAGCAGAT AGGCCTGAGC 
CAACACTCTG GTAACGTGGA AAACTCAACA GAGAGAAAAG GAAGCTCTCT AGAGAGACAA 
CAAGCAGCCA ACTTAGAAGA CACAAAGAGC TCATTGGATT GTCCAGCTAA AACTGTTCTA 
GAGTGGCAGC GTCAAACCCA GGACTGGATA AACTCTACAG AACGCCAAGA AGAAACTCGG 
AAAGCCCCAC ACAAACAAGG GGTTGGATCA GAGGAGTCCC TGGTGCCCTA CAGCAAGCCC 
AGCTTCCCGT CTCCTGGCGG GCACAGCTCA TCAGGAACAT CTTCCTCTAA AGGGTCCACT 
GGCCCTCGGA AGGCTGACGT GCTGAGAGGC AGCCACCAGA GGAATGCCAA TGACCTTCTC 
GACATTGGAT ATGTGGGCTC AAATAGTCAA GGACAGTTTA CAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo2 ( XM_006523035.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523098.1 Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X1, mRNA.

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CloneID	OMu52755
Clone ID Related Accession (Same CDS sequence)	XM_006523035.4 , XM_006523035.3
Accession Version	XM_006523035.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4797bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	roundabout homolog 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006523035.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT 
CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG 
GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC 
CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA 
GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT 
ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG 
GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG 
CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT 
AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT 
TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC 
GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC 
CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT 
ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA 
CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA 
TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA 
GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT 
CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT 
GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG 
CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG 
GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC 
ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG 
GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT 
GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA 
GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT 
GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG 
GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT 
GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT 
CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG 
CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG 
CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT 
TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG 
GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT 
GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT 
GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGCCT TACTTTACAT CCCTGACTAC 
CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC 
ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA 
AAGAAGAAAA CTAAAAATTC TTCGAAAGCG CAGAAAAACA ACGGATCCAC TTGGGCTAAT 
GTCCCTCTAC CTCCTCCTCC CGTCCAGCCC CTTCCTGGTA CAGAGCTGGG CCACTATGCT 
GCGGAACAAG AAAATGGCTA TGACAGTGAC AGCTGGTGCC CACCATTACC GGTGCAAACA 
TACTTGCATC AGGGTATGGA AGATGAGCTG GAAGAAGACG AAGATCGGGT CCCAACACCT 
CCTGTTCGCG GCGTGGCCTC TTCACCAGCT ATCTCTTTTG GACAGCAGTC CACTGCCACT 
CTTACTCCAT CCCCACGGGA AGAGATGCAA CCCATGCTGC AAGCTCACTT GGATGAGTTG 
ACAAGGGCCT ATCAGTTTGA TATAGCAAAA CAAACATGGC ACATTCAAAG CAATACCCCA 
CCTCCACAAC CCCCAGCTCC GCCATTAGGT TACGTGTCCG GAGCCTTGAT TTCTGATTTG 
GAGACAGATG TTCCAGATGA GGATGCTGAT GATGAAGAGG AACCATTAGA AATTCCCAGG 
CCCCTCAGAG CACTAGACCA GACACCTGGA TCCAGTATGG ACAATCTAGA CAGCTCTGTC 
ACAGGTTCAA TGGTAAATGG ATGGGGCTCT GCATCTGATG AGGATCGTAA CTTTTCTAGT 
CATAGATCTA GTGTAGGTAG CTCCTCAGAT GGCTCCATCT TTGCCAGCGG CAGCTTTGCA 
AAAGCATTGG TGGCAGCAGC AGACAAAGCT GGCTTTAGGC TGGATGGAAC CAGTCTTACG 
AGAACAGGAA AAGCCTTTAG CTCCTCTCAA AGGCAGCGGC CCACCAGCCC ATTTTCTACG 
GACAGTAACA CCAGTGCTGC CCAGAATCAA AGCCAGAGGC CTCGGCCCAC GAAAAAACAT 
AAGGGAGGAC GGATGGACCC ACAGCCAGTG TTACCTCATC GGAGGGAAGG GATGCCAGAT 
GATCTTCCCC CACCACCAGA TCCCCCACCA GGTCAGGGTT TAAGGCAGCA GATAGGCCTG 
AGCCAACACT CTGGTAACGT GGAAAACTCA ACAGAGAGAA AAGGAAGCTC TCTAGAGAGA 
CAACAAGCAG CCAACTTAGA AGACACAAAG AGCTCATTGG ATTGTCCAGC TAAAACTGTT 
CTAGAGTGGC AGCGTCAAAC CCAGGACTGG ATAAACTCTA CAGAACGCCA AGAAGAAACT 
CGGAAAGCCC CACACAAACA AGGGGTTGGA TCAGAGGAGT CCCTGGTGCC CTACAGCAAG 
CCCAGCTTCC CGTCTCCTGG CGGGCACAGC TCATCAGGAA CATCTTCCTC TAAAGGGTCC 
ACTGGCCCTC GGAAGGCTGA CGTGCTGAGA GGCAGCCACC AGAGGAATGC CAATGACCTT 
CTCGACATTG GATATGTGGG CTCAAATAGT CAAGGACAGT TTACAGGTGA ATTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523098.1
CDS	9440..14236
Translation	MLSSEKVDKITRLELLFVLMLFFSGPTLGKLTRTPWKGKPWKGGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKAMSTDEGTYVCIAENRVGKVEASATLTVRVRPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPINQTLAVDGTALLKCKATGEPLPVISWLKEGFTFLGRDPRATIQDQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDMNDLPGPPSKPQVTDVSKNSVTLSWQPGTPGVLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVVVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQASTVWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSHNSTSISVSWDPPPADHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVVIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGGRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPNYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTTYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSVQQKTDLMGFGYSLPDQNKGNNALLYIPDYRLAEGLSNRMPHNQSQDFSTTSSHNSSERSGSLSGGKGGKKKKTKNSSKAQKNNGSTWANVPLPPPPVQPLPGTELGHYAAEQENGYDSDSWCPPLPVQTYLHQGMEDELEEDEDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNTPPPQPPAPPLGYVSGALISDLETDVPDEDADDEEEPLEIPRPLRALDQTPGSSMDNLDSSVTGSMVNGWGSASDEDRNFSSHRSSVGSSSDGSIFASGSFAKALVAAADKAGFRLDGTSLTRTGKAFSSSQRQRPTSPFSTDSNTSAAQNQSQRPRPTKKHKGGRMDPQPVLPHRREGMPDDLPPPPDPPPGQGLRQQIGLSQHSGNVENSTERKGSSLERQQAANLEDTKSSLDCPAKTVLEWQRQTQDWINSTERQEETRKAPHKQGVGSEESLVPYSKPSFPSPGGHSSSGTSSSKGSTGPRKADVLRGSHQRNANDLLDIGYVGSNSQGQFTGEL

Target ORF information:

RefSeq Version	XM_006523035.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523035.4

ORF Insert Sequence:

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT 
CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG 
GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC 
CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA 
GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT 
ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG 
GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG 
CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT 
AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT 
TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC 
GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC 
CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT 
ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA 
CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA 
TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA 
GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT 
CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT 
GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG 
CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG 
GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC 
ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG 
GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT 
GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA 
GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT 
GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG 
GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT 
GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT 
CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG 
CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG 
CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT 
TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG 
GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT 
GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT 
GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGCCT TACTTTACAT CCCTGACTAC 
CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC 
ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA 
AAGAAGAAAA CTAAAAATTC TTCGAAAGCG CAGAAAAACA ACGGATCCAC TTGGGCTAAT 
GTCCCTCTAC CTCCTCCTCC CGTCCAGCCC CTTCCTGGTA CAGAGCTGGG CCACTATGCT 
GCGGAACAAG AAAATGGCTA TGACAGTGAC AGCTGGTGCC CACCATTACC GGTGCAAACA 
TACTTGCATC AGGGTATGGA AGATGAGCTG GAAGAAGACG AAGATCGGGT CCCAACACCT 
CCTGTTCGCG GCGTGGCCTC TTCACCAGCT ATCTCTTTTG GACAGCAGTC CACTGCCACT 
CTTACTCCAT CCCCACGGGA AGAGATGCAA CCCATGCTGC AAGCTCACTT GGATGAGTTG 
ACAAGGGCCT ATCAGTTTGA TATAGCAAAA CAAACATGGC ACATTCAAAG CAATACCCCA 
CCTCCACAAC CCCCAGCTCC GCCATTAGGT TACGTGTCCG GAGCCTTGAT TTCTGATTTG 
GAGACAGATG TTCCAGATGA GGATGCTGAT GATGAAGAGG AACCATTAGA AATTCCCAGG 
CCCCTCAGAG CACTAGACCA GACACCTGGA TCCAGTATGG ACAATCTAGA CAGCTCTGTC 
ACAGGTTCAA TGGTAAATGG ATGGGGCTCT GCATCTGATG AGGATCGTAA CTTTTCTAGT 
CATAGATCTA GTGTAGGTAG CTCCTCAGAT GGCTCCATCT TTGCCAGCGG CAGCTTTGCA 
AAAGCATTGG TGGCAGCAGC AGACAAAGCT GGCTTTAGGC TGGATGGAAC CAGTCTTACG 
AGAACAGGAA AAGCCTTTAG CTCCTCTCAA AGGCAGCGGC CCACCAGCCC ATTTTCTACG 
GACAGTAACA CCAGTGCTGC CCAGAATCAA AGCCAGAGGC CTCGGCCCAC GAAAAAACAT 
AAGGGAGGAC GGATGGACCC ACAGCCAGTG TTACCTCATC GGAGGGAAGG GATGCCAGAT 
GATCTTCCCC CACCACCAGA TCCCCCACCA GGTCAGGGTT TAAGGCAGCA GATAGGCCTG 
AGCCAACACT CTGGTAACGT GGAAAACTCA ACAGAGAGAA AAGGAAGCTC TCTAGAGAGA 
CAACAAGCAG CCAACTTAGA AGACACAAAG AGCTCATTGG ATTGTCCAGC TAAAACTGTT 
CTAGAGTGGC AGCGTCAAAC CCAGGACTGG ATAAACTCTA CAGAACGCCA AGAAGAAACT 
CGGAAAGCCC CACACAAACA AGGGGTTGGA TCAGAGGAGT CCCTGGTGCC CTACAGCAAG 
CCCAGCTTCC CGTCTCCTGG CGGGCACAGC TCATCAGGAA CATCTTCCTC TAAAGGGTCC 
ACTGGCCCTC GGAAGGCTGA CGTGCTGAGA GGCAGCCACC AGAGGAATGC CAATGACCTT 
CTCGACATTG GATATGTGGG CTCAAATAGT CAAGGACAGT TTACAGGTGA ATTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo2 ( XM_006523036.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523099.1 Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X2, mRNA.

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CloneID	OMu52756
Clone ID Related Accession (Same CDS sequence)	XM_006523036.3 , XM_006523036.4
Accession Version	XM_006523036.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	roundabout homolog 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006523036.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT 
CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG 
GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC 
CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA 
GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT 
ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG 
GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG 
CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT 
AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT 
TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC 
GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC 
CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT 
ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA 
CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA 
TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA 
GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT 
CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT 
GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG 
CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG 
GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC 
ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG 
GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT 
GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA 
GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT 
GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG 
GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT 
GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT 
CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG 
CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG 
CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT 
TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG 
GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT 
GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT 
GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGCCT TACTTTACAT CCCTGACTAC 
CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC 
ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA 
AAGAAGAAAA CTAAAAATTC TTCGAAAGCG CAGAAAAACA ACGGATCCAC TTGGGCTAAT 
GTCCCTCTAC CTCCTCCTCC CGTCCAGCCC CTTCCTGGTA CAGAGCTGGG CCACTATGCT 
GCGGAACAAG AAAATGGCTA TGACAGTGAC AGCTGGTGCC CACCATTACC GGTGCAAACA 
TACTTGCATC AGGGTATGGA AGATGAGCTG GAAGAAGACG AAGATCGGGT CCCAACACCT 
CCTGTTCGCG GCGTGGCCTC TTCACCAGCT ATCTCTTTTG GACAGCAGTC CACTGCCACT 
CTTACTCCAT CCCCACGGGA AGAGATGCAA CCCATGCTGC AAGCTCACTT GGATGAGTTG 
ACAAGGGCCT ATCAGTTTGA TATAGCAAAA CAAACATGGC ACATTCAAAG CAATACCCCA 
CCTCCACAAC CCCCAGCTCC GCCATTAGGT TACGTGTCCG GAGCCTTGAT TTCTGATTTG 
GAGACAGATG TTCCAGATGA GGATGCTGAT GATGAAGAGG AACCATTAGA AATTCCCAGG 
CCCCTCAGAG CACTAGACCA GACACCTGGA TCCAGTATGG ACAATCTAGA CAGCTCTGTC 
ACAGGTTCAA TGGTAAATGG ATGGGGCTCT GCATCTGATG AGGATCGTAA CTTTTCTAGT 
CATAGATCTA GTGTAGGTAG CTCCTCAGAT GGCTCCATCT TTGCCAGCGG CAGCTTTGCA 
AAAGCATTGG TGGCAGCAGC AGACAAAGCT GGCTTTAGGC TGGATGGAAC CAGTCTTACG 
AGAACAGGAA AAGCCTTTAG CTCCTCTCAA AGGCAGCGGC CCACCAGCCC ATTTTCTACG 
GACAGTAACA CCAGTGCTGC CCAGAATCAA AGCCAGAGGC CTCGGCCCAC GAAAAAACAT 
AAGGGAGGAC GGATGGACCC ACAGCCAGTG TTACCTCATC GGAGGGAAGG GATGCCAGAT 
GATCTTCCCC CACCACCAGA TCCCCCACCA GGTCAGGGTT TAAGGCAGCA GATAGGCCTG 
AGCCAACACT CTGGTAACGT GGAAAACTCA ACAGAGAGAA AAGGAAGCTC TCTAGAGAGA 
CAACAAGCAG CCAACTTAGA AGACACAAAG AGCTCATTGG ATTGTCCAGC TAAAACTGTT 
CTAGAGTGGC AGCGTCAAAC CCAGGACTGG ATAAACTCTA CAGAACGCCA AGAAGAAACT 
CGGAAAGCCC CACACAAACA AGGGGTTGGA TCAGAGGAGT CCCTGGTGCC CTACAGCAAG 
CCCAGCTTCC CGTCTCCTGG CGGGCACAGC TCATCAGGAA CATCTTCCTC TAAAGGGTCC 
ACTGGCCCTC GGAAGGCTGA CGTGCTGAGA GGCAGCCACC AGAGGAATGC CAATGACCTT 
CTCGACATTG GATATGTGGG CTCAAATAGT CAAGGACAGT TTACAGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523099.1
CDS	9440..14230
Translation	MLSSEKVDKITRLELLFVLMLFFSGPTLGKLTRTPWKGKPWKGGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKAMSTDEGTYVCIAENRVGKVEASATLTVRVRPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPINQTLAVDGTALLKCKATGEPLPVISWLKEGFTFLGRDPRATIQDQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDMNDLPGPPSKPQVTDVSKNSVTLSWQPGTPGVLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVVVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQASTVWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSHNSTSISVSWDPPPADHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVVIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGGRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPNYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTTYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSVQQKTDLMGFGYSLPDQNKGNNALLYIPDYRLAEGLSNRMPHNQSQDFSTTSSHNSSERSGSLSGGKGGKKKKTKNSSKAQKNNGSTWANVPLPPPPVQPLPGTELGHYAAEQENGYDSDSWCPPLPVQTYLHQGMEDELEEDEDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNTPPPQPPAPPLGYVSGALISDLETDVPDEDADDEEEPLEIPRPLRALDQTPGSSMDNLDSSVTGSMVNGWGSASDEDRNFSSHRSSVGSSSDGSIFASGSFAKALVAAADKAGFRLDGTSLTRTGKAFSSSQRQRPTSPFSTDSNTSAAQNQSQRPRPTKKHKGGRMDPQPVLPHRREGMPDDLPPPPDPPPGQGLRQQIGLSQHSGNVENSTERKGSSLERQQAANLEDTKSSLDCPAKTVLEWQRQTQDWINSTERQEETRKAPHKQGVGSEESLVPYSKPSFPSPGGHSSSGTSSSKGSTGPRKADVLRGSHQRNANDLLDIGYVGSNSQGQFTE

Target ORF information:

RefSeq Version	XM_006523036.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523036.4

ORF Insert Sequence:

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT 
CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG 
GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC 
CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA 
GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT 
ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG 
GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG 
CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT 
AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT 
TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC 
GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC 
CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT 
ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA 
CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA 
TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA 
GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT 
CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT 
GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG 
CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG 
GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC 
ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG 
GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT 
GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA 
GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT 
GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG 
GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT 
GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT 
CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG 
CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG 
CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT 
TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG 
GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT 
GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT 
GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGCCT TACTTTACAT CCCTGACTAC 
CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC 
ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA 
AAGAAGAAAA CTAAAAATTC TTCGAAAGCG CAGAAAAACA ACGGATCCAC TTGGGCTAAT 
GTCCCTCTAC CTCCTCCTCC CGTCCAGCCC CTTCCTGGTA CAGAGCTGGG CCACTATGCT 
GCGGAACAAG AAAATGGCTA TGACAGTGAC AGCTGGTGCC CACCATTACC GGTGCAAACA 
TACTTGCATC AGGGTATGGA AGATGAGCTG GAAGAAGACG AAGATCGGGT CCCAACACCT 
CCTGTTCGCG GCGTGGCCTC TTCACCAGCT ATCTCTTTTG GACAGCAGTC CACTGCCACT 
CTTACTCCAT CCCCACGGGA AGAGATGCAA CCCATGCTGC AAGCTCACTT GGATGAGTTG 
ACAAGGGCCT ATCAGTTTGA TATAGCAAAA CAAACATGGC ACATTCAAAG CAATACCCCA 
CCTCCACAAC CCCCAGCTCC GCCATTAGGT TACGTGTCCG GAGCCTTGAT TTCTGATTTG 
GAGACAGATG TTCCAGATGA GGATGCTGAT GATGAAGAGG AACCATTAGA AATTCCCAGG 
CCCCTCAGAG CACTAGACCA GACACCTGGA TCCAGTATGG ACAATCTAGA CAGCTCTGTC 
ACAGGTTCAA TGGTAAATGG ATGGGGCTCT GCATCTGATG AGGATCGTAA CTTTTCTAGT 
CATAGATCTA GTGTAGGTAG CTCCTCAGAT GGCTCCATCT TTGCCAGCGG CAGCTTTGCA 
AAAGCATTGG TGGCAGCAGC AGACAAAGCT GGCTTTAGGC TGGATGGAAC CAGTCTTACG 
AGAACAGGAA AAGCCTTTAG CTCCTCTCAA AGGCAGCGGC CCACCAGCCC ATTTTCTACG 
GACAGTAACA CCAGTGCTGC CCAGAATCAA AGCCAGAGGC CTCGGCCCAC GAAAAAACAT 
AAGGGAGGAC GGATGGACCC ACAGCCAGTG TTACCTCATC GGAGGGAAGG GATGCCAGAT 
GATCTTCCCC CACCACCAGA TCCCCCACCA GGTCAGGGTT TAAGGCAGCA GATAGGCCTG 
AGCCAACACT CTGGTAACGT GGAAAACTCA ACAGAGAGAA AAGGAAGCTC TCTAGAGAGA 
CAACAAGCAG CCAACTTAGA AGACACAAAG AGCTCATTGG ATTGTCCAGC TAAAACTGTT 
CTAGAGTGGC AGCGTCAAAC CCAGGACTGG ATAAACTCTA CAGAACGCCA AGAAGAAACT 
CGGAAAGCCC CACACAAACA AGGGGTTGGA TCAGAGGAGT CCCTGGTGCC CTACAGCAAG 
CCCAGCTTCC CGTCTCCTGG CGGGCACAGC TCATCAGGAA CATCTTCCTC TAAAGGGTCC 
ACTGGCCCTC GGAAGGCTGA CGTGCTGAGA GGCAGCCACC AGAGGAATGC CAATGACCTT 
CTCGACATTG GATATGTGGG CTCAAATAGT CAAGGACAGT TTACAGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo2 ( XM_006523037.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523100.1 Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X3, mRNA.

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CloneID	OMu52757
Clone ID Related Accession (Same CDS sequence)	XM_006523037.4 , XM_006523037.3
Accession Version	XM_006523037.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4785bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	roundabout homolog 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006523037.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGCTCCTCC ACAGTTTGTG 
GTTAGGCCAA GAGATCAGAT CGTTGCTCAA GGCCGAACAG TGACATTCCC CTGTGAAACT 
AAAGGAAACC CACAGCCAGC TGTTTTTTGG CAGAAAGAAG GCAGCCAGAA CCTACTTTTC 
CCGAATCAAC CTCAGCAGCC CAACAGCCGA TGTTCAGTGT CGCCCACGGG GGACCTCACC 
ATCACCAACA TCCAGCGTTC AGATGCGGGT TACTACATCT GCCAGGCCCT AACCGTGGCA 
GGAAGCATTT TAGCTAAAGC ACAGTTGGAA GTTACTGACG TTTTGACAGA TAGACCTCCA 
CCCATAATCT TGCAAGGACC AATAAACCAA ACACTTGCAG TAGACGGTAC AGCATTGTTG 
AAGTGTAAAG CCACTGGTGA GCCTCTGCCT GTAATTAGCT GGCTAAAGGA GGGCTTTACT 
TTTCTGGGGA GAGATCCAAG AGCCACGATC CAAGACCAAG GAACACTGCA GATTAAGAAT 
TTACGGATAT CTGATACTGG CACTTATACT TGTGTGGCTA CAAGTTCCAG TGGAGAGACT 
TCCTGGAGTG CAGTGCTGGA TGTAACAGAA TCTGGAGCAA CAATCAGTAA AAATTATGAT 
ATGAATGACC TCCCGGGACC ACCATCCAAA CCTCAGGTCA CTGATGTTTC TAAGAACAGT 
GTCACCTTAT CCTGGCAGCC AGGTACACCT GGCGTTCTTC CTGCAAGCGC GTATATCATT 
GAGGCTTTCA GCCAATCGGT GAGCAATAGC TGGCAGACAG TGGCAAACCA TGTTAAGACA 
ACTCTGTATA CAGTAAGGGG GCTGAGGCCC AACACAATCT ACTTGTTTAT GGTCAGAGCG 
ATCAACCCAC AAGGTCTCAG TGATCCAAGT CCTATGTCGG ATCCTGTACG CACACAAGAT 
ATCAGCCCCC CAGCACAAGG AGTGGACCAC AGACAGGTGC AGAAGGAATT AGGTGATGTC 
GTTGTTCGTC TCCATAATCC AGTTGTCCTG ACACCTACAA CTGTTCAAGT CACATGGACG 
GTGGACCGAC AACCCCAGTT TATTCAGGGC TACAGAGTGA TGTACCGTCA GACTTCGGGA 
CTACAAGCCT CAACTGTGTG GCAGAATCTA GACGCCAAAG TCCCGACTGA GAGGAGTGCT 
GTCCTTGTGA ATTTGAAAAA GGGGGTGACT TATGAAATTA AAGTCCGGCC GTATTTTAAC 
GAGTTCCAAG GAATGGACAG TGAATCGAAA ACAGTCCGAA CCACTGAGGA AGCCCCAAGT 
GCCCCTCCCC AGTCTGTCAC TGTGCTGACA GTTGGAAGTC ACAACAGCAC AAGCATCAGT 
GTTTCCTGGG ATCCTCCACC AGCCGACCAC CAGAATGGAA TTATTCAGGA ATATAAGATC 
TGGTGTCTGG GAAACGAAAC GCGATTCCAT ATCAATAAAA CGGTGGATGC AGCCATTCGC 
TCTGTAGTAA TAGGTGGCTT GTTCCCTGGA ATTCAGTACC GGGTAGAAGT GGCAGCTAGC 
ACAAGTGCAG GGGTTGGAGT AAAAAGTGAA CCACAGCCGA TAATAATTGG GGGACGTAAC 
GAAGTTGTCA TTACTGAAAA CAATAACAGC ATCACTGAGC AAATCACGGA TGTCGTGAAG 
CAACCGGCAT TTATAGCTGG CATTGGTGGT GCCTGCTGGG TAATTCTGAT GGGTTTTAGC 
ATCTGGTTGT ACTGGAGAAG AAAGAAGAGA AAGGGACTCA GCAATTATGC TGTAACATTT 
CAAAGAGGAG ATGGAGGACT AATGAGCAAT GGGAGCCGTC CAGGTCTTCT AAATGCTGGC 
GATCCCAATT ACCCATGGCT TGCTGATTCT TGGCCAGCCA CGAGTTTGCC AGTGAACAAT 
AGCAATAGTG GCCCAAATGA AATTGGAAAT TTTGGGCGTG GAGATGTGCT GCCTCCGGTG 
CCAGGCCAAG GGGATAAAAC AGCGACCATG CTCTCGGATG GAGCCATTTA TAGCAGCATT 
GACTTCACTA CCAAAACCAC TTACAACAGT TCCAGCCAAA TAACACAGGC CACCCCATAT 
GCCACTACAC AAATCCTGCA TTCAAACAGC ATCCACGAAC TGGCAGTTGA TCTTCCTGAT 
CCACAGTGGA AAAGCTCAGT TCAACAGAAG ACAGACCTCA TGGGATTTGG TTATTCGCTA 
CCTGATCAGA ACAAGGGGAA CAACGCCTTA CTTTACATCC CTGACTACCG ATTGGCTGAG 
GGATTGTCTA ATAGAATGCC ACACAACCAG TCACAGGATT TCAGCACCAC CAGCTCTCAC 
AACAGCTCAG AAAGGAGTGG CAGTCTCTCA GGTGGGAAAG GTGGAAAAAA GAAGAAAACT 
AAAAATTCTT CGAAAGCGCA GAAAAACAAC GGATCCACTT GGGCTAATGT CCCTCTACCT 
CCTCCTCCCG TCCAGCCCCT TCCTGGTACA GAGCTGGGCC ACTATGCTGC GGAACAAGAA 
AATGGCTATG ACAGTGACAG CTGGTGCCCA CCATTACCGG TGCAAACATA CTTGCATCAG 
GGTATGGAAG ATGAGCTGGA AGAAGACGAA GATCGGGTCC CAACACCTCC TGTTCGCGGC 
GTGGCCTCTT CACCAGCTAT CTCTTTTGGA CAGCAGTCCA CTGCCACTCT TACTCCATCC 
CCACGGGAAG AGATGCAACC CATGCTGCAA GCTCACTTGG ATGAGTTGAC AAGGGCCTAT 
CAGTTTGATA TAGCAAAACA AACATGGCAC ATTCAAAGCA ATACCCCACC TCCACAACCC 
CCAGCTCCGC CATTAGGTTA CGTGTCCGGA GCCTTGATTT CTGATTTGGA GACAGATGTT 
CCAGATGAGG ATGCTGATGA TGAAGAGGAA CCATTAGAAA TTCCCAGGCC CCTCAGAGCA 
CTAGACCAGA CACCTGGATC CAGTATGGAC AATCTAGACA GCTCTGTCAC AGGTTCAATG 
GTAAATGGAT GGGGCTCTGC ATCTGATGAG GATCGTAACT TTTCTAGTCA TAGATCTAGT 
GTAGGTAGCT CCTCAGATGG CTCCATCTTT GCCAGCGGCA GCTTTGCAAA AGCATTGGTG 
GCAGCAGCAG ACAAAGCTGG CTTTAGGCTG GATGGAACCA GTCTTACGAG AACAGGAAAA 
GCCTTTAGCT CCTCTCAAAG GCAGCGGCCC ACCAGCCCAT TTTCTACGGA CAGTAACACC 
AGTGCTGCCC AGAATCAAAG CCAGAGGCCT CGGCCCACGA AAAAACATAA GGGAGGACGG 
ATGGACCCAC AGCCAGTGTT ACCTCATCGG AGGGAAGGGA TGCCAGATGA TCTTCCCCCA 
CCACCAGATC CCCCACCAGG TCAGGGTTTA AGGCAGCAGA TAGGCCTGAG CCAACACTCT 
GGTAACGTGG AAAACTCAAC AGAGAGAAAA GGAAGCTCTC TAGAGAGACA ACAAGCAGCC 
AACTTAGAAG ACACAAAGAG CTCATTGGAT TGTCCAGCTA AAACTGTTCT AGAGTGGCAG 
CGTCAAACCC AGGACTGGAT AAACTCTACA GAACGCCAAG AAGAAACTCG GAAAGCCCCA 
CACAAACAAG GGGTTGGATC AGAGGAGTCC CTGGTGCCCT ACAGCAAGCC CAGCTTCCCG 
TCTCCTGGCG GGCACAGCTC ATCAGGAACA TCTTCCTCTA AAGGGTCCAC TGGCCCTCGG 
AAGGCTGACG TGCTGAGAGG CAGCCACCAG AGGAATGCCA ATGACCTTCT CGACATTGGA 
TATGTGGGCT CAAATAGTCA AGGACAGTTT ACAGGTGAAT TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523100.1
CDS	9440..14224
Translation	MLSSEKVDKITRLELLFVLMLFFSGPTLGKLTRTPWKGKPWKGGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKAMSTDEGTYVCIAENRVGKVEASATLTVRAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPINQTLAVDGTALLKCKATGEPLPVISWLKEGFTFLGRDPRATIQDQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDMNDLPGPPSKPQVTDVSKNSVTLSWQPGTPGVLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVVVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQASTVWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSHNSTSISVSWDPPPADHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVVIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGGRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPNYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTTYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSVQQKTDLMGFGYSLPDQNKGNNALLYIPDYRLAEGLSNRMPHNQSQDFSTTSSHNSSERSGSLSGGKGGKKKKTKNSSKAQKNNGSTWANVPLPPPPVQPLPGTELGHYAAEQENGYDSDSWCPPLPVQTYLHQGMEDELEEDEDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNTPPPQPPAPPLGYVSGALISDLETDVPDEDADDEEEPLEIPRPLRALDQTPGSSMDNLDSSVTGSMVNGWGSASDEDRNFSSHRSSVGSSSDGSIFASGSFAKALVAAADKAGFRLDGTSLTRTGKAFSSSQRQRPTSPFSTDSNTSAAQNQSQRPRPTKKHKGGRMDPQPVLPHRREGMPDDLPPPPDPPPGQGLRQQIGLSQHSGNVENSTERKGSSLERQQAANLEDTKSSLDCPAKTVLEWQRQTQDWINSTERQEETRKAPHKQGVGSEESLVPYSKPSFPSPGGHSSSGTSSSKGSTGPRKADVLRGSHQRNANDLLDIGYVGSNSQGQFTGEL

Target ORF information:

RefSeq Version	XM_006523037.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523037.4

ORF Insert Sequence:

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGCTCCTCC ACAGTTTGTG 
GTTAGGCCAA GAGATCAGAT CGTTGCTCAA GGCCGAACAG TGACATTCCC CTGTGAAACT 
AAAGGAAACC CACAGCCAGC TGTTTTTTGG CAGAAAGAAG GCAGCCAGAA CCTACTTTTC 
CCGAATCAAC CTCAGCAGCC CAACAGCCGA TGTTCAGTGT CGCCCACGGG GGACCTCACC 
ATCACCAACA TCCAGCGTTC AGATGCGGGT TACTACATCT GCCAGGCCCT AACCGTGGCA 
GGAAGCATTT TAGCTAAAGC ACAGTTGGAA GTTACTGACG TTTTGACAGA TAGACCTCCA 
CCCATAATCT TGCAAGGACC AATAAACCAA ACACTTGCAG TAGACGGTAC AGCATTGTTG 
AAGTGTAAAG CCACTGGTGA GCCTCTGCCT GTAATTAGCT GGCTAAAGGA GGGCTTTACT 
TTTCTGGGGA GAGATCCAAG AGCCACGATC CAAGACCAAG GAACACTGCA GATTAAGAAT 
TTACGGATAT CTGATACTGG CACTTATACT TGTGTGGCTA CAAGTTCCAG TGGAGAGACT 
TCCTGGAGTG CAGTGCTGGA TGTAACAGAA TCTGGAGCAA CAATCAGTAA AAATTATGAT 
ATGAATGACC TCCCGGGACC ACCATCCAAA CCTCAGGTCA CTGATGTTTC TAAGAACAGT 
GTCACCTTAT CCTGGCAGCC AGGTACACCT GGCGTTCTTC CTGCAAGCGC GTATATCATT 
GAGGCTTTCA GCCAATCGGT GAGCAATAGC TGGCAGACAG TGGCAAACCA TGTTAAGACA 
ACTCTGTATA CAGTAAGGGG GCTGAGGCCC AACACAATCT ACTTGTTTAT GGTCAGAGCG 
ATCAACCCAC AAGGTCTCAG TGATCCAAGT CCTATGTCGG ATCCTGTACG CACACAAGAT 
ATCAGCCCCC CAGCACAAGG AGTGGACCAC AGACAGGTGC AGAAGGAATT AGGTGATGTC 
GTTGTTCGTC TCCATAATCC AGTTGTCCTG ACACCTACAA CTGTTCAAGT CACATGGACG 
GTGGACCGAC AACCCCAGTT TATTCAGGGC TACAGAGTGA TGTACCGTCA GACTTCGGGA 
CTACAAGCCT CAACTGTGTG GCAGAATCTA GACGCCAAAG TCCCGACTGA GAGGAGTGCT 
GTCCTTGTGA ATTTGAAAAA GGGGGTGACT TATGAAATTA AAGTCCGGCC GTATTTTAAC 
GAGTTCCAAG GAATGGACAG TGAATCGAAA ACAGTCCGAA CCACTGAGGA AGCCCCAAGT 
GCCCCTCCCC AGTCTGTCAC TGTGCTGACA GTTGGAAGTC ACAACAGCAC AAGCATCAGT 
GTTTCCTGGG ATCCTCCACC AGCCGACCAC CAGAATGGAA TTATTCAGGA ATATAAGATC 
TGGTGTCTGG GAAACGAAAC GCGATTCCAT ATCAATAAAA CGGTGGATGC AGCCATTCGC 
TCTGTAGTAA TAGGTGGCTT GTTCCCTGGA ATTCAGTACC GGGTAGAAGT GGCAGCTAGC 
ACAAGTGCAG GGGTTGGAGT AAAAAGTGAA CCACAGCCGA TAATAATTGG GGGACGTAAC 
GAAGTTGTCA TTACTGAAAA CAATAACAGC ATCACTGAGC AAATCACGGA TGTCGTGAAG 
CAACCGGCAT TTATAGCTGG CATTGGTGGT GCCTGCTGGG TAATTCTGAT GGGTTTTAGC 
ATCTGGTTGT ACTGGAGAAG AAAGAAGAGA AAGGGACTCA GCAATTATGC TGTAACATTT 
CAAAGAGGAG ATGGAGGACT AATGAGCAAT GGGAGCCGTC CAGGTCTTCT AAATGCTGGC 
GATCCCAATT ACCCATGGCT TGCTGATTCT TGGCCAGCCA CGAGTTTGCC AGTGAACAAT 
AGCAATAGTG GCCCAAATGA AATTGGAAAT TTTGGGCGTG GAGATGTGCT GCCTCCGGTG 
CCAGGCCAAG GGGATAAAAC AGCGACCATG CTCTCGGATG GAGCCATTTA TAGCAGCATT 
GACTTCACTA CCAAAACCAC TTACAACAGT TCCAGCCAAA TAACACAGGC CACCCCATAT 
GCCACTACAC AAATCCTGCA TTCAAACAGC ATCCACGAAC TGGCAGTTGA TCTTCCTGAT 
CCACAGTGGA AAAGCTCAGT TCAACAGAAG ACAGACCTCA TGGGATTTGG TTATTCGCTA 
CCTGATCAGA ACAAGGGGAA CAACGCCTTA CTTTACATCC CTGACTACCG ATTGGCTGAG 
GGATTGTCTA ATAGAATGCC ACACAACCAG TCACAGGATT TCAGCACCAC CAGCTCTCAC 
AACAGCTCAG AAAGGAGTGG CAGTCTCTCA GGTGGGAAAG GTGGAAAAAA GAAGAAAACT 
AAAAATTCTT CGAAAGCGCA GAAAAACAAC GGATCCACTT GGGCTAATGT CCCTCTACCT 
CCTCCTCCCG TCCAGCCCCT TCCTGGTACA GAGCTGGGCC ACTATGCTGC GGAACAAGAA 
AATGGCTATG ACAGTGACAG CTGGTGCCCA CCATTACCGG TGCAAACATA CTTGCATCAG 
GGTATGGAAG ATGAGCTGGA AGAAGACGAA GATCGGGTCC CAACACCTCC TGTTCGCGGC 
GTGGCCTCTT CACCAGCTAT CTCTTTTGGA CAGCAGTCCA CTGCCACTCT TACTCCATCC 
CCACGGGAAG AGATGCAACC CATGCTGCAA GCTCACTTGG ATGAGTTGAC AAGGGCCTAT 
CAGTTTGATA TAGCAAAACA AACATGGCAC ATTCAAAGCA ATACCCCACC TCCACAACCC 
CCAGCTCCGC CATTAGGTTA CGTGTCCGGA GCCTTGATTT CTGATTTGGA GACAGATGTT 
CCAGATGAGG ATGCTGATGA TGAAGAGGAA CCATTAGAAA TTCCCAGGCC CCTCAGAGCA 
CTAGACCAGA CACCTGGATC CAGTATGGAC AATCTAGACA GCTCTGTCAC AGGTTCAATG 
GTAAATGGAT GGGGCTCTGC ATCTGATGAG GATCGTAACT TTTCTAGTCA TAGATCTAGT 
GTAGGTAGCT CCTCAGATGG CTCCATCTTT GCCAGCGGCA GCTTTGCAAA AGCATTGGTG 
GCAGCAGCAG ACAAAGCTGG CTTTAGGCTG GATGGAACCA GTCTTACGAG AACAGGAAAA 
GCCTTTAGCT CCTCTCAAAG GCAGCGGCCC ACCAGCCCAT TTTCTACGGA CAGTAACACC 
AGTGCTGCCC AGAATCAAAG CCAGAGGCCT CGGCCCACGA AAAAACATAA GGGAGGACGG 
ATGGACCCAC AGCCAGTGTT ACCTCATCGG AGGGAAGGGA TGCCAGATGA TCTTCCCCCA 
CCACCAGATC CCCCACCAGG TCAGGGTTTA AGGCAGCAGA TAGGCCTGAG CCAACACTCT 
GGTAACGTGG AAAACTCAAC AGAGAGAAAA GGAAGCTCTC TAGAGAGACA ACAAGCAGCC 
AACTTAGAAG ACACAAAGAG CTCATTGGAT TGTCCAGCTA AAACTGTTCT AGAGTGGCAG 
CGTCAAACCC AGGACTGGAT AAACTCTACA GAACGCCAAG AAGAAACTCG GAAAGCCCCA 
CACAAACAAG GGGTTGGATC AGAGGAGTCC CTGGTGCCCT ACAGCAAGCC CAGCTTCCCG 
TCTCCTGGCG GGCACAGCTC ATCAGGAACA TCTTCCTCTA AAGGGTCCAC TGGCCCTCGG 
AAGGCTGACG TGCTGAGAGG CAGCCACCAG AGGAATGCCA ATGACCTTCT CGACATTGGA 
TATGTGGGCT CAAATAGTCA AGGACAGTTT ACAGGTGAAT TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo2 ( XM_006523038.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523101.1 Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu52758
Clone ID Related Accession (Same CDS sequence)	XM_006523038.3 , XM_006523038.4 , XM_030249140.1 , XM_030249139.1
Accession Version	XM_006523038.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4773bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	roundabout homolog 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006523038.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGAA GACAAGAAAG TATCTGTGGA AGGCCGTGGA CGTGGACGCC AGGACTACTG 
ATGCTGATCA TCCTTGGCAT TCATCAGGGG AGTGGGCAAG GTCAAGGATC TCGTCTTCGT 
CAAGAAGACT TTCCCCCCAG GATCGTAGAA CACCCTTCTG ATGTCATCGT CTCCAAGGGA 
GAGCCCACCA CTCTGAACTG TAAAGCAGAG GGCCGACCCA CCCCCACCAT TGAATGGTAC 
AAGGATGGTG AGAGGGTGGA GACAGACAAG GATGATCCCA GGTCCCACAG AATGCTTCTG 
CCCAGCGGAT CTTTATTCTT TTTGCGAATT GTTCATGGGC GCAGAAGTAA ACCGGACGAA 
GGGAGTTACG TTTGTGTTGC AAGGAACTAT CTTGGTGAAG CAGTGAGTCG AAATGCATCT 
CTGGAAGTGG CATTATTGCG AGATGACTTC CGGCAAAACC CCACAGATGT GGTAGTCGCA 
GCTGGAGAGC CTGCAATCTT GGAGTGCCAG CCACCACGGG GACACCCAGA ACCAACCATC 
TACTGGAAAA AGGACAAAGT CCGAATTGAT GACAAGGAAG AGAGAATAAG TATCCGTGGT 
GGGAAGCTGA TGATCTCTAA TACTAGGAAA AGCGATGCTG GCATGTACAC CTGTGTGGGA 
ACCAATATGG TGGGAGAAAG GGACAGCGAC CCTGCAGAGC TCACTGTCTT TGAACGACCC 
ACATTTCTCA GGAGGCCAAT TAACCAGGTG GTGCTAGAGG AAGAAGCTGT AGAATTCCGT 
TGTCAGGTCC AAGGAGATCC CCAGCCAACG GTGAGGTGGA AAAAAGATGA TGCAGACTTG 
CCGAGAGGAA GGTATGATAT CAAAGATGAC TACACGCTGA GAATTAAAAA GGCCATGAGT 
ACTGATGAAG GTACCTATGT GTGTATTGCT GAGAATCGGG TGGGAAAAGT GGAAGCCTCT 
GCTACCCTCA CTGTCCGAGT TCGCCCTGTT GCTCCTCCAC AGTTTGTGGT TAGGCCAAGA 
GATCAGATCG TTGCTCAAGG CCGAACAGTG ACATTCCCCT GTGAAACTAA AGGAAACCCA 
CAGCCAGCTG TTTTTTGGCA GAAAGAAGGC AGCCAGAACC TACTTTTCCC GAATCAACCT 
CAGCAGCCCA ACAGCCGATG TTCAGTGTCG CCCACGGGGG ACCTCACCAT CACCAACATC 
CAGCGTTCAG ATGCGGGTTA CTACATCTGC CAGGCCCTAA CCGTGGCAGG AAGCATTTTA 
GCTAAAGCAC AGTTGGAAGT TACTGACGTT TTGACAGATA GACCTCCACC CATAATCTTG 
CAAGGACCAA TAAACCAAAC ACTTGCAGTA GACGGTACAG CATTGTTGAA GTGTAAAGCC 
ACTGGTGAGC CTCTGCCTGT AATTAGCTGG CTAAAGGAGG GCTTTACTTT TCTGGGGAGA 
GATCCAAGAG CCACGATCCA AGACCAAGGA ACACTGCAGA TTAAGAATTT ACGGATATCT 
GATACTGGCA CTTATACTTG TGTGGCTACA AGTTCCAGTG GAGAGACTTC CTGGAGTGCA 
GTGCTGGATG TAACAGAATC TGGAGCAACA ATCAGTAAAA ATTATGATAT GAATGACCTC 
CCGGGACCAC CATCCAAACC TCAGGTCACT GATGTTTCTA AGAACAGTGT CACCTTATCC 
TGGCAGCCAG GTACACCTGG CGTTCTTCCT GCAAGCGCGT ATATCATTGA GGCTTTCAGC 
CAATCGGTGA GCAATAGCTG GCAGACAGTG GCAAACCATG TTAAGACAAC TCTGTATACA 
GTAAGGGGGC TGAGGCCCAA CACAATCTAC TTGTTTATGG TCAGAGCGAT CAACCCACAA 
GGTCTCAGTG ATCCAAGTCC TATGTCGGAT CCTGTACGCA CACAAGATAT CAGCCCCCCA 
GCACAAGGAG TGGACCACAG ACAGGTGCAG AAGGAATTAG GTGATGTCGT TGTTCGTCTC 
CATAATCCAG TTGTCCTGAC ACCTACAACT GTTCAAGTCA CATGGACGGT GGACCGACAA 
CCCCAGTTTA TTCAGGGCTA CAGAGTGATG TACCGTCAGA CTTCGGGACT ACAAGCCTCA 
ACTGTGTGGC AGAATCTAGA CGCCAAAGTC CCGACTGAGA GGAGTGCTGT CCTTGTGAAT 
TTGAAAAAGG GGGTGACTTA TGAAATTAAA GTCCGGCCGT ATTTTAACGA GTTCCAAGGA 
ATGGACAGTG AATCGAAAAC AGTCCGAACC ACTGAGGAAG CCCCAAGTGC CCCTCCCCAG 
TCTGTCACTG TGCTGACAGT TGGAAGTCAC AACAGCACAA GCATCAGTGT TTCCTGGGAT 
CCTCCACCAG CCGACCACCA GAATGGAATT ATTCAGGAAT ATAAGATCTG GTGTCTGGGA 
AACGAAACGC GATTCCATAT CAATAAAACG GTGGATGCAG CCATTCGCTC TGTAGTAATA 
GGTGGCTTGT TCCCTGGAAT TCAGTACCGG GTAGAAGTGG CAGCTAGCAC AAGTGCAGGG 
GTTGGAGTAA AAAGTGAACC ACAGCCGATA ATAATTGGGG GACGTAACGA AGTTGTCATT 
ACTGAAAACA ATAACAGCAT CACTGAGCAA ATCACGGATG TCGTGAAGCA ACCGGCATTT 
ATAGCTGGCA TTGGTGGTGC CTGCTGGGTA ATTCTGATGG GTTTTAGCAT CTGGTTGTAC 
TGGAGAAGAA AGAAGAGAAA GGGACTCAGC AATTATGCTG TAACATTTCA AAGAGGAGAT 
GGAGGACTAA TGAGCAATGG GAGCCGTCCA GGTCTTCTAA ATGCTGGCGA TCCCAATTAC 
CCATGGCTTG CTGATTCTTG GCCAGCCACG AGTTTGCCAG TGAACAATAG CAATAGTGGC 
CCAAATGAAA TTGGAAATTT TGGGCGTGGA GATGTGCTGC CTCCGGTGCC AGGCCAAGGG 
GATAAAACAG CGACCATGCT CTCGGATGGA GCCATTTATA GCAGCATTGA CTTCACTACC 
AAAACCACTT ACAACAGTTC CAGCCAAATA ACACAGGCCA CCCCATATGC CACTACACAA 
ATCCTGCATT CAAACAGCAT CCACGAACTG GCAGTTGATC TTCCTGATCC ACAGTGGAAA 
AGCTCAGTTC AACAGAAGAC AGACCTCATG GGATTTGGTT ATTCGCTACC TGATCAGAAC 
AAGGGGAACA ACGCCTTACT TTACATCCCT GACTACCGAT TGGCTGAGGG ATTGTCTAAT 
AGAATGCCAC ACAACCAGTC ACAGGATTTC AGCACCACCA GCTCTCACAA CAGCTCAGAA 
AGGAGTGGCA GTCTCTCAGG TGGGAAAGGT GGAAAAAAGA AGAAAACTAA AAATTCTTCG 
AAAGCGCAGA AAAACAACGG ATCCACTTGG GCTAATGTCC CTCTACCTCC TCCTCCCGTC 
CAGCCCCTTC CTGGTACAGA GCTGGGCCAC TATGCTGCGG AACAAGAAAA TGGCTATGAC 
AGTGACAGCT GGTGCCCACC ATTACCGGTG CAAACATACT TGCATCAGGG TATGGAAGAT 
GAGCTGGAAG AAGACGAAGA TCGGGTCCCA ACACCTCCTG TTCGCGGCGT GGCCTCTTCA 
CCAGCTATCT CTTTTGGACA GCAGTCCACT GCCACTCTTA CTCCATCCCC ACGGGAAGAG 
ATGCAACCCA TGCTGCAAGC TCACTTGGAT GAGTTGACAA GGGCCTATCA GTTTGATATA 
GCAAAACAAA CATGGCACAT TCAAAGCAAT ACCCCACCTC CACAACCCCC AGCTCCGCCA 
TTAGGTTACG TGTCCGGAGC CTTGATTTCT GATTTGGAGA CAGATGTTCC AGATGAGGAT 
GCTGATGATG AAGAGGAACC ATTAGAAATT CCCAGGCCCC TCAGAGCACT AGACCAGACA 
CCTGGATCCA GTATGGACAA TCTAGACAGC TCTGTCACAG GTTCAATGGT AAATGGATGG 
GGCTCTGCAT CTGATGAGGA TCGTAACTTT TCTAGTCATA GATCTAGTGT AGGTAGCTCC 
TCAGATGGCT CCATCTTTGC CAGCGGCAGC TTTGCAAAAG CATTGGTGGC AGCAGCAGAC 
AAAGCTGGCT TTAGGCTGGA TGGAACCAGT CTTACGAGAA CAGGAAAAGC CTTTAGCTCC 
TCTCAAAGGC AGCGGCCCAC CAGCCCATTT TCTACGGACA GTAACACCAG TGCTGCCCAG 
AATCAAAGCC AGAGGCCTCG GCCCACGAAA AAACATAAGG GAGGACGGAT GGACCCACAG 
CCAGTGTTAC CTCATCGGAG GGAAGGGATG CCAGATGATC TTCCCCCACC ACCAGATCCC 
CCACCAGGTC AGGGTTTAAG GCAGCAGATA GGCCTGAGCC AACACTCTGG TAACGTGGAA 
AACTCAACAG AGAGAAAAGG AAGCTCTCTA GAGAGACAAC AAGCAGCCAA CTTAGAAGAC 
ACAAAGAGCT CATTGGATTG TCCAGCTAAA ACTGTTCTAG AGTGGCAGCG TCAAACCCAG 
GACTGGATAA ACTCTACAGA ACGCCAAGAA GAAACTCGGA AAGCCCCACA CAAACAAGGG 
GTTGGATCAG AGGAGTCCCT GGTGCCCTAC AGCAAGCCCA GCTTCCCGTC TCCTGGCGGG 
CACAGCTCAT CAGGAACATC TTCCTCTAAA GGGTCCACTG GCCCTCGGAA GGCTGACGTG 
CTGAGAGGCA GCCACCAGAG GAATGCCAAT GACCTTCTCG ACATTGGATA TGTGGGCTCA 
AATAGTCAAG GACAGTTTAC AGGTGAATTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523101.1
CDS	386..5158
Translation	MARRQESICGRPWTWTPGLLMLIILGIHQGSGQGQGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKAMSTDEGTYVCIAENRVGKVEASATLTVRVRPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPINQTLAVDGTALLKCKATGEPLPVISWLKEGFTFLGRDPRATIQDQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDMNDLPGPPSKPQVTDVSKNSVTLSWQPGTPGVLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVVVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQASTVWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSHNSTSISVSWDPPPADHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVVIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGGRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPNYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTTYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSVQQKTDLMGFGYSLPDQNKGNNALLYIPDYRLAEGLSNRMPHNQSQDFSTTSSHNSSERSGSLSGGKGGKKKKTKNSSKAQKNNGSTWANVPLPPPPVQPLPGTELGHYAAEQENGYDSDSWCPPLPVQTYLHQGMEDELEEDEDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNTPPPQPPAPPLGYVSGALISDLETDVPDEDADDEEEPLEIPRPLRALDQTPGSSMDNLDSSVTGSMVNGWGSASDEDRNFSSHRSSVGSSSDGSIFASGSFAKALVAAADKAGFRLDGTSLTRTGKAFSSSQRQRPTSPFSTDSNTSAAQNQSQRPRPTKKHKGGRMDPQPVLPHRREGMPDDLPPPPDPPPGQGLRQQIGLSQHSGNVENSTERKGSSLERQQAANLEDTKSSLDCPAKTVLEWQRQTQDWINSTERQEETRKAPHKQGVGSEESLVPYSKPSFPSPGGHSSSGTSSSKGSTGPRKADVLRGSHQRNANDLLDIGYVGSNSQGQFTGEL

Target ORF information:

RefSeq Version	XM_006523038.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523038.4

ORF Insert Sequence:

ATGGCCAGAA GACAAGAAAG TATCTGTGGA AGGCCGTGGA CGTGGACGCC AGGACTACTG 
ATGCTGATCA TCCTTGGCAT TCATCAGGGG AGTGGGCAAG GTCAAGGATC TCGTCTTCGT 
CAAGAAGACT TTCCCCCCAG GATCGTAGAA CACCCTTCTG ATGTCATCGT CTCCAAGGGA 
GAGCCCACCA CTCTGAACTG TAAAGCAGAG GGCCGACCCA CCCCCACCAT TGAATGGTAC 
AAGGATGGTG AGAGGGTGGA GACAGACAAG GATGATCCCA GGTCCCACAG AATGCTTCTG 
CCCAGCGGAT CTTTATTCTT TTTGCGAATT GTTCATGGGC GCAGAAGTAA ACCGGACGAA 
GGGAGTTACG TTTGTGTTGC AAGGAACTAT CTTGGTGAAG CAGTGAGTCG AAATGCATCT 
CTGGAAGTGG CATTATTGCG AGATGACTTC CGGCAAAACC CCACAGATGT GGTAGTCGCA 
GCTGGAGAGC CTGCAATCTT GGAGTGCCAG CCACCACGGG GACACCCAGA ACCAACCATC 
TACTGGAAAA AGGACAAAGT CCGAATTGAT GACAAGGAAG AGAGAATAAG TATCCGTGGT 
GGGAAGCTGA TGATCTCTAA TACTAGGAAA AGCGATGCTG GCATGTACAC CTGTGTGGGA 
ACCAATATGG TGGGAGAAAG GGACAGCGAC CCTGCAGAGC TCACTGTCTT TGAACGACCC 
ACATTTCTCA GGAGGCCAAT TAACCAGGTG GTGCTAGAGG AAGAAGCTGT AGAATTCCGT 
TGTCAGGTCC AAGGAGATCC CCAGCCAACG GTGAGGTGGA AAAAAGATGA TGCAGACTTG 
CCGAGAGGAA GGTATGATAT CAAAGATGAC TACACGCTGA GAATTAAAAA GGCCATGAGT 
ACTGATGAAG GTACCTATGT GTGTATTGCT GAGAATCGGG TGGGAAAAGT GGAAGCCTCT 
GCTACCCTCA CTGTCCGAGT TCGCCCTGTT GCTCCTCCAC AGTTTGTGGT TAGGCCAAGA 
GATCAGATCG TTGCTCAAGG CCGAACAGTG ACATTCCCCT GTGAAACTAA AGGAAACCCA 
CAGCCAGCTG TTTTTTGGCA GAAAGAAGGC AGCCAGAACC TACTTTTCCC GAATCAACCT 
CAGCAGCCCA ACAGCCGATG TTCAGTGTCG CCCACGGGGG ACCTCACCAT CACCAACATC 
CAGCGTTCAG ATGCGGGTTA CTACATCTGC CAGGCCCTAA CCGTGGCAGG AAGCATTTTA 
GCTAAAGCAC AGTTGGAAGT TACTGACGTT TTGACAGATA GACCTCCACC CATAATCTTG 
CAAGGACCAA TAAACCAAAC ACTTGCAGTA GACGGTACAG CATTGTTGAA GTGTAAAGCC 
ACTGGTGAGC CTCTGCCTGT AATTAGCTGG CTAAAGGAGG GCTTTACTTT TCTGGGGAGA 
GATCCAAGAG CCACGATCCA AGACCAAGGA ACACTGCAGA TTAAGAATTT ACGGATATCT 
GATACTGGCA CTTATACTTG TGTGGCTACA AGTTCCAGTG GAGAGACTTC CTGGAGTGCA 
GTGCTGGATG TAACAGAATC TGGAGCAACA ATCAGTAAAA ATTATGATAT GAATGACCTC 
CCGGGACCAC CATCCAAACC TCAGGTCACT GATGTTTCTA AGAACAGTGT CACCTTATCC 
TGGCAGCCAG GTACACCTGG CGTTCTTCCT GCAAGCGCGT ATATCATTGA GGCTTTCAGC 
CAATCGGTGA GCAATAGCTG GCAGACAGTG GCAAACCATG TTAAGACAAC TCTGTATACA 
GTAAGGGGGC TGAGGCCCAA CACAATCTAC TTGTTTATGG TCAGAGCGAT CAACCCACAA 
GGTCTCAGTG ATCCAAGTCC TATGTCGGAT CCTGTACGCA CACAAGATAT CAGCCCCCCA 
GCACAAGGAG TGGACCACAG ACAGGTGCAG AAGGAATTAG GTGATGTCGT TGTTCGTCTC 
CATAATCCAG TTGTCCTGAC ACCTACAACT GTTCAAGTCA CATGGACGGT GGACCGACAA 
CCCCAGTTTA TTCAGGGCTA CAGAGTGATG TACCGTCAGA CTTCGGGACT ACAAGCCTCA 
ACTGTGTGGC AGAATCTAGA CGCCAAAGTC CCGACTGAGA GGAGTGCTGT CCTTGTGAAT 
TTGAAAAAGG GGGTGACTTA TGAAATTAAA GTCCGGCCGT ATTTTAACGA GTTCCAAGGA 
ATGGACAGTG AATCGAAAAC AGTCCGAACC ACTGAGGAAG CCCCAAGTGC CCCTCCCCAG 
TCTGTCACTG TGCTGACAGT TGGAAGTCAC AACAGCACAA GCATCAGTGT TTCCTGGGAT 
CCTCCACCAG CCGACCACCA GAATGGAATT ATTCAGGAAT ATAAGATCTG GTGTCTGGGA 
AACGAAACGC GATTCCATAT CAATAAAACG GTGGATGCAG CCATTCGCTC TGTAGTAATA 
GGTGGCTTGT TCCCTGGAAT TCAGTACCGG GTAGAAGTGG CAGCTAGCAC AAGTGCAGGG 
GTTGGAGTAA AAAGTGAACC ACAGCCGATA ATAATTGGGG GACGTAACGA AGTTGTCATT 
ACTGAAAACA ATAACAGCAT CACTGAGCAA ATCACGGATG TCGTGAAGCA ACCGGCATTT 
ATAGCTGGCA TTGGTGGTGC CTGCTGGGTA ATTCTGATGG GTTTTAGCAT CTGGTTGTAC 
TGGAGAAGAA AGAAGAGAAA GGGACTCAGC AATTATGCTG TAACATTTCA AAGAGGAGAT 
GGAGGACTAA TGAGCAATGG GAGCCGTCCA GGTCTTCTAA ATGCTGGCGA TCCCAATTAC 
CCATGGCTTG CTGATTCTTG GCCAGCCACG AGTTTGCCAG TGAACAATAG CAATAGTGGC 
CCAAATGAAA TTGGAAATTT TGGGCGTGGA GATGTGCTGC CTCCGGTGCC AGGCCAAGGG 
GATAAAACAG CGACCATGCT CTCGGATGGA GCCATTTATA GCAGCATTGA CTTCACTACC 
AAAACCACTT ACAACAGTTC CAGCCAAATA ACACAGGCCA CCCCATATGC CACTACACAA 
ATCCTGCATT CAAACAGCAT CCACGAACTG GCAGTTGATC TTCCTGATCC ACAGTGGAAA 
AGCTCAGTTC AACAGAAGAC AGACCTCATG GGATTTGGTT ATTCGCTACC TGATCAGAAC 
AAGGGGAACA ACGCCTTACT TTACATCCCT GACTACCGAT TGGCTGAGGG ATTGTCTAAT 
AGAATGCCAC ACAACCAGTC ACAGGATTTC AGCACCACCA GCTCTCACAA CAGCTCAGAA 
AGGAGTGGCA GTCTCTCAGG TGGGAAAGGT GGAAAAAAGA AGAAAACTAA AAATTCTTCG 
AAAGCGCAGA AAAACAACGG ATCCACTTGG GCTAATGTCC CTCTACCTCC TCCTCCCGTC 
CAGCCCCTTC CTGGTACAGA GCTGGGCCAC TATGCTGCGG AACAAGAAAA TGGCTATGAC 
AGTGACAGCT GGTGCCCACC ATTACCGGTG CAAACATACT TGCATCAGGG TATGGAAGAT 
GAGCTGGAAG AAGACGAAGA TCGGGTCCCA ACACCTCCTG TTCGCGGCGT GGCCTCTTCA 
CCAGCTATCT CTTTTGGACA GCAGTCCACT GCCACTCTTA CTCCATCCCC ACGGGAAGAG 
ATGCAACCCA TGCTGCAAGC TCACTTGGAT GAGTTGACAA GGGCCTATCA GTTTGATATA 
GCAAAACAAA CATGGCACAT TCAAAGCAAT ACCCCACCTC CACAACCCCC AGCTCCGCCA 
TTAGGTTACG TGTCCGGAGC CTTGATTTCT GATTTGGAGA CAGATGTTCC AGATGAGGAT 
GCTGATGATG AAGAGGAACC ATTAGAAATT CCCAGGCCCC TCAGAGCACT AGACCAGACA 
CCTGGATCCA GTATGGACAA TCTAGACAGC TCTGTCACAG GTTCAATGGT AAATGGATGG 
GGCTCTGCAT CTGATGAGGA TCGTAACTTT TCTAGTCATA GATCTAGTGT AGGTAGCTCC 
TCAGATGGCT CCATCTTTGC CAGCGGCAGC TTTGCAAAAG CATTGGTGGC AGCAGCAGAC 
AAAGCTGGCT TTAGGCTGGA TGGAACCAGT CTTACGAGAA CAGGAAAAGC CTTTAGCTCC 
TCTCAAAGGC AGCGGCCCAC CAGCCCATTT TCTACGGACA GTAACACCAG TGCTGCCCAG 
AATCAAAGCC AGAGGCCTCG GCCCACGAAA AAACATAAGG GAGGACGGAT GGACCCACAG 
CCAGTGTTAC CTCATCGGAG GGAAGGGATG CCAGATGATC TTCCCCCACC ACCAGATCCC 
CCACCAGGTC AGGGTTTAAG GCAGCAGATA GGCCTGAGCC AACACTCTGG TAACGTGGAA 
AACTCAACAG AGAGAAAAGG AAGCTCTCTA GAGAGACAAC AAGCAGCCAA CTTAGAAGAC 
ACAAAGAGCT CATTGGATTG TCCAGCTAAA ACTGTTCTAG AGTGGCAGCG TCAAACCCAG 
GACTGGATAA ACTCTACAGA ACGCCAAGAA GAAACTCGGA AAGCCCCACA CAAACAAGGG 
GTTGGATCAG AGGAGTCCCT GGTGCCCTAC AGCAAGCCCA GCTTCCCGTC TCCTGGCGGG 
CACAGCTCAT CAGGAACATC TTCCTCTAAA GGGTCCACTG GCCCTCGGAA GGCTGACGTG 
CTGAGAGGCA GCCACCAGAG GAATGCCAAT GACCTTCTCG ACATTGGATA TGTGGGCTCA 
AATAGTCAAG GACAGTTTAC AGGTGAATTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo2 ( XM_030249140.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105000.1 Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OMu52758
Clone ID Related Accession (Same CDS sequence)	XM_006523038.3 , XM_006523038.4 , XM_030249140.1 , XM_030249139.1
Accession Version	XM_030249140.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4773bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	roundabout homolog 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGAA GACAAGAAAG TATCTGTGGA AGGCCGTGGA CGTGGACGCC AGGACTACTG 
ATGCTGATCA TCCTTGGCAT TCATCAGGGG AGTGGGCAAG GTCAAGGATC TCGTCTTCGT 
CAAGAAGACT TTCCCCCCAG GATCGTAGAA CACCCTTCTG ATGTCATCGT CTCCAAGGGA 
GAGCCCACCA CTCTGAACTG TAAAGCAGAG GGCCGACCCA CCCCCACCAT TGAATGGTAC 
AAGGATGGTG AGAGGGTGGA GACAGACAAG GATGATCCCA GGTCCCACAG AATGCTTCTG 
CCCAGCGGAT CTTTATTCTT TTTGCGAATT GTTCATGGGC GCAGAAGTAA ACCGGACGAA 
GGGAGTTACG TTTGTGTTGC AAGGAACTAT CTTGGTGAAG CAGTGAGTCG AAATGCATCT 
CTGGAAGTGG CATTATTGCG AGATGACTTC CGGCAAAACC CCACAGATGT GGTAGTCGCA 
GCTGGAGAGC CTGCAATCTT GGAGTGCCAG CCACCACGGG GACACCCAGA ACCAACCATC 
TACTGGAAAA AGGACAAAGT CCGAATTGAT GACAAGGAAG AGAGAATAAG TATCCGTGGT 
GGGAAGCTGA TGATCTCTAA TACTAGGAAA AGCGATGCTG GCATGTACAC CTGTGTGGGA 
ACCAATATGG TGGGAGAAAG GGACAGCGAC CCTGCAGAGC TCACTGTCTT TGAACGACCC 
ACATTTCTCA GGAGGCCAAT TAACCAGGTG GTGCTAGAGG AAGAAGCTGT AGAATTCCGT 
TGTCAGGTCC AAGGAGATCC CCAGCCAACG GTGAGGTGGA AAAAAGATGA TGCAGACTTG 
CCGAGAGGAA GGTATGATAT CAAAGATGAC TACACGCTGA GAATTAAAAA GGCCATGAGT 
ACTGATGAAG GTACCTATGT GTGTATTGCT GAGAATCGGG TGGGAAAAGT GGAAGCCTCT 
GCTACCCTCA CTGTCCGAGT TCGCCCTGTT GCTCCTCCAC AGTTTGTGGT TAGGCCAAGA 
GATCAGATCG TTGCTCAAGG CCGAACAGTG ACATTCCCCT GTGAAACTAA AGGAAACCCA 
CAGCCAGCTG TTTTTTGGCA GAAAGAAGGC AGCCAGAACC TACTTTTCCC GAATCAACCT 
CAGCAGCCCA ACAGCCGATG TTCAGTGTCG CCCACGGGGG ACCTCACCAT CACCAACATC 
CAGCGTTCAG ATGCGGGTTA CTACATCTGC CAGGCCCTAA CCGTGGCAGG AAGCATTTTA 
GCTAAAGCAC AGTTGGAAGT TACTGACGTT TTGACAGATA GACCTCCACC CATAATCTTG 
CAAGGACCAA TAAACCAAAC ACTTGCAGTA GACGGTACAG CATTGTTGAA GTGTAAAGCC 
ACTGGTGAGC CTCTGCCTGT AATTAGCTGG CTAAAGGAGG GCTTTACTTT TCTGGGGAGA 
GATCCAAGAG CCACGATCCA AGACCAAGGA ACACTGCAGA TTAAGAATTT ACGGATATCT 
GATACTGGCA CTTATACTTG TGTGGCTACA AGTTCCAGTG GAGAGACTTC CTGGAGTGCA 
GTGCTGGATG TAACAGAATC TGGAGCAACA ATCAGTAAAA ATTATGATAT GAATGACCTC 
CCGGGACCAC CATCCAAACC TCAGGTCACT GATGTTTCTA AGAACAGTGT CACCTTATCC 
TGGCAGCCAG GTACACCTGG CGTTCTTCCT GCAAGCGCGT ATATCATTGA GGCTTTCAGC 
CAATCGGTGA GCAATAGCTG GCAGACAGTG GCAAACCATG TTAAGACAAC TCTGTATACA 
GTAAGGGGGC TGAGGCCCAA CACAATCTAC TTGTTTATGG TCAGAGCGAT CAACCCACAA 
GGTCTCAGTG ATCCAAGTCC TATGTCGGAT CCTGTACGCA CACAAGATAT CAGCCCCCCA 
GCACAAGGAG TGGACCACAG ACAGGTGCAG AAGGAATTAG GTGATGTCGT TGTTCGTCTC 
CATAATCCAG TTGTCCTGAC ACCTACAACT GTTCAAGTCA CATGGACGGT GGACCGACAA 
CCCCAGTTTA TTCAGGGCTA CAGAGTGATG TACCGTCAGA CTTCGGGACT ACAAGCCTCA 
ACTGTGTGGC AGAATCTAGA CGCCAAAGTC CCGACTGAGA GGAGTGCTGT CCTTGTGAAT 
TTGAAAAAGG GGGTGACTTA TGAAATTAAA GTCCGGCCGT ATTTTAACGA GTTCCAAGGA 
ATGGACAGTG AATCGAAAAC AGTCCGAACC ACTGAGGAAG CCCCAAGTGC CCCTCCCCAG 
TCTGTCACTG TGCTGACAGT TGGAAGTCAC AACAGCACAA GCATCAGTGT TTCCTGGGAT 
CCTCCACCAG CCGACCACCA GAATGGAATT ATTCAGGAAT ATAAGATCTG GTGTCTGGGA 
AACGAAACGC GATTCCATAT CAATAAAACG GTGGATGCAG CCATTCGCTC TGTAGTAATA 
GGTGGCTTGT TCCCTGGAAT TCAGTACCGG GTAGAAGTGG CAGCTAGCAC AAGTGCAGGG 
GTTGGAGTAA AAAGTGAACC ACAGCCGATA ATAATTGGGG GACGTAACGA AGTTGTCATT 
ACTGAAAACA ATAACAGCAT CACTGAGCAA ATCACGGATG TCGTGAAGCA ACCGGCATTT 
ATAGCTGGCA TTGGTGGTGC CTGCTGGGTA ATTCTGATGG GTTTTAGCAT CTGGTTGTAC 
TGGAGAAGAA AGAAGAGAAA GGGACTCAGC AATTATGCTG TAACATTTCA AAGAGGAGAT 
GGAGGACTAA TGAGCAATGG GAGCCGTCCA GGTCTTCTAA ATGCTGGCGA TCCCAATTAC 
CCATGGCTTG CTGATTCTTG GCCAGCCACG AGTTTGCCAG TGAACAATAG CAATAGTGGC 
CCAAATGAAA TTGGAAATTT TGGGCGTGGA GATGTGCTGC CTCCGGTGCC AGGCCAAGGG 
GATAAAACAG CGACCATGCT CTCGGATGGA GCCATTTATA GCAGCATTGA CTTCACTACC 
AAAACCACTT ACAACAGTTC CAGCCAAATA ACACAGGCCA CCCCATATGC CACTACACAA 
ATCCTGCATT CAAACAGCAT CCACGAACTG GCAGTTGATC TTCCTGATCC ACAGTGGAAA 
AGCTCAGTTC AACAGAAGAC AGACCTCATG GGATTTGGTT ATTCGCTACC TGATCAGAAC 
AAGGGGAACA ACGCCTTACT TTACATCCCT GACTACCGAT TGGCTGAGGG ATTGTCTAAT 
AGAATGCCAC ACAACCAGTC ACAGGATTTC AGCACCACCA GCTCTCACAA CAGCTCAGAA 
AGGAGTGGCA GTCTCTCAGG TGGGAAAGGT GGAAAAAAGA AGAAAACTAA AAATTCTTCG 
AAAGCGCAGA AAAACAACGG ATCCACTTGG GCTAATGTCC CTCTACCTCC TCCTCCCGTC 
CAGCCCCTTC CTGGTACAGA GCTGGGCCAC TATGCTGCGG AACAAGAAAA TGGCTATGAC 
AGTGACAGCT GGTGCCCACC ATTACCGGTG CAAACATACT TGCATCAGGG TATGGAAGAT 
GAGCTGGAAG AAGACGAAGA TCGGGTCCCA ACACCTCCTG TTCGCGGCGT GGCCTCTTCA 
CCAGCTATCT CTTTTGGACA GCAGTCCACT GCCACTCTTA CTCCATCCCC ACGGGAAGAG 
ATGCAACCCA TGCTGCAAGC TCACTTGGAT GAGTTGACAA GGGCCTATCA GTTTGATATA 
GCAAAACAAA CATGGCACAT TCAAAGCAAT ACCCCACCTC CACAACCCCC AGCTCCGCCA 
TTAGGTTACG TGTCCGGAGC CTTGATTTCT GATTTGGAGA CAGATGTTCC AGATGAGGAT 
GCTGATGATG AAGAGGAACC ATTAGAAATT CCCAGGCCCC TCAGAGCACT AGACCAGACA 
CCTGGATCCA GTATGGACAA TCTAGACAGC TCTGTCACAG GTTCAATGGT AAATGGATGG 
GGCTCTGCAT CTGATGAGGA TCGTAACTTT TCTAGTCATA GATCTAGTGT AGGTAGCTCC 
TCAGATGGCT CCATCTTTGC CAGCGGCAGC TTTGCAAAAG CATTGGTGGC AGCAGCAGAC 
AAAGCTGGCT TTAGGCTGGA TGGAACCAGT CTTACGAGAA CAGGAAAAGC CTTTAGCTCC 
TCTCAAAGGC AGCGGCCCAC CAGCCCATTT TCTACGGACA GTAACACCAG TGCTGCCCAG 
AATCAAAGCC AGAGGCCTCG GCCCACGAAA AAACATAAGG GAGGACGGAT GGACCCACAG 
CCAGTGTTAC CTCATCGGAG GGAAGGGATG CCAGATGATC TTCCCCCACC ACCAGATCCC 
CCACCAGGTC AGGGTTTAAG GCAGCAGATA GGCCTGAGCC AACACTCTGG TAACGTGGAA 
AACTCAACAG AGAGAAAAGG AAGCTCTCTA GAGAGACAAC AAGCAGCCAA CTTAGAAGAC 
ACAAAGAGCT CATTGGATTG TCCAGCTAAA ACTGTTCTAG AGTGGCAGCG TCAAACCCAG 
GACTGGATAA ACTCTACAGA ACGCCAAGAA GAAACTCGGA AAGCCCCACA CAAACAAGGG 
GTTGGATCAG AGGAGTCCCT GGTGCCCTAC AGCAAGCCCA GCTTCCCGTC TCCTGGCGGG 
CACAGCTCAT CAGGAACATC TTCCTCTAAA GGGTCCACTG GCCCTCGGAA GGCTGACGTG 
CTGAGAGGCA GCCACCAGAG GAATGCCAAT GACCTTCTCG ACATTGGATA TGTGGGCTCA 
AATAGTCAAG GACAGTTTAC AGGTGAATTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105000.1
CDS	112..4884
Translation	MARRQESICGRPWTWTPGLLMLIILGIHQGSGQGQGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKAMSTDEGTYVCIAENRVGKVEASATLTVRVRPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPINQTLAVDGTALLKCKATGEPLPVISWLKEGFTFLGRDPRATIQDQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDMNDLPGPPSKPQVTDVSKNSVTLSWQPGTPGVLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVVVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQASTVWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSHNSTSISVSWDPPPADHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVVIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGGRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPNYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTTYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSVQQKTDLMGFGYSLPDQNKGNNALLYIPDYRLAEGLSNRMPHNQSQDFSTTSSHNSSERSGSLSGGKGGKKKKTKNSSKAQKNNGSTWANVPLPPPPVQPLPGTELGHYAAEQENGYDSDSWCPPLPVQTYLHQGMEDELEEDEDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNTPPPQPPAPPLGYVSGALISDLETDVPDEDADDEEEPLEIPRPLRALDQTPGSSMDNLDSSVTGSMVNGWGSASDEDRNFSSHRSSVGSSSDGSIFASGSFAKALVAAADKAGFRLDGTSLTRTGKAFSSSQRQRPTSPFSTDSNTSAAQNQSQRPRPTKKHKGGRMDPQPVLPHRREGMPDDLPPPPDPPPGQGLRQQIGLSQHSGNVENSTERKGSSLERQQAANLEDTKSSLDCPAKTVLEWQRQTQDWINSTERQEETRKAPHKQGVGSEESLVPYSKPSFPSPGGHSSSGTSSSKGSTGPRKADVLRGSHQRNANDLLDIGYVGSNSQGQFTGEL

Target ORF information:

RefSeq Version	XM_030249140.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249140.1

ORF Insert Sequence:

ATGGCCAGAA GACAAGAAAG TATCTGTGGA AGGCCGTGGA CGTGGACGCC AGGACTACTG 
ATGCTGATCA TCCTTGGCAT TCATCAGGGG AGTGGGCAAG GTCAAGGATC TCGTCTTCGT 
CAAGAAGACT TTCCCCCCAG GATCGTAGAA CACCCTTCTG ATGTCATCGT CTCCAAGGGA 
GAGCCCACCA CTCTGAACTG TAAAGCAGAG GGCCGACCCA CCCCCACCAT TGAATGGTAC 
AAGGATGGTG AGAGGGTGGA GACAGACAAG GATGATCCCA GGTCCCACAG AATGCTTCTG 
CCCAGCGGAT CTTTATTCTT TTTGCGAATT GTTCATGGGC GCAGAAGTAA ACCGGACGAA 
GGGAGTTACG TTTGTGTTGC AAGGAACTAT CTTGGTGAAG CAGTGAGTCG AAATGCATCT 
CTGGAAGTGG CATTATTGCG AGATGACTTC CGGCAAAACC CCACAGATGT GGTAGTCGCA 
GCTGGAGAGC CTGCAATCTT GGAGTGCCAG CCACCACGGG GACACCCAGA ACCAACCATC 
TACTGGAAAA AGGACAAAGT CCGAATTGAT GACAAGGAAG AGAGAATAAG TATCCGTGGT 
GGGAAGCTGA TGATCTCTAA TACTAGGAAA AGCGATGCTG GCATGTACAC CTGTGTGGGA 
ACCAATATGG TGGGAGAAAG GGACAGCGAC CCTGCAGAGC TCACTGTCTT TGAACGACCC 
ACATTTCTCA GGAGGCCAAT TAACCAGGTG GTGCTAGAGG AAGAAGCTGT AGAATTCCGT 
TGTCAGGTCC AAGGAGATCC CCAGCCAACG GTGAGGTGGA AAAAAGATGA TGCAGACTTG 
CCGAGAGGAA GGTATGATAT CAAAGATGAC TACACGCTGA GAATTAAAAA GGCCATGAGT 
ACTGATGAAG GTACCTATGT GTGTATTGCT GAGAATCGGG TGGGAAAAGT GGAAGCCTCT 
GCTACCCTCA CTGTCCGAGT TCGCCCTGTT GCTCCTCCAC AGTTTGTGGT TAGGCCAAGA 
GATCAGATCG TTGCTCAAGG CCGAACAGTG ACATTCCCCT GTGAAACTAA AGGAAACCCA 
CAGCCAGCTG TTTTTTGGCA GAAAGAAGGC AGCCAGAACC TACTTTTCCC GAATCAACCT 
CAGCAGCCCA ACAGCCGATG TTCAGTGTCG CCCACGGGGG ACCTCACCAT CACCAACATC 
CAGCGTTCAG ATGCGGGTTA CTACATCTGC CAGGCCCTAA CCGTGGCAGG AAGCATTTTA 
GCTAAAGCAC AGTTGGAAGT TACTGACGTT TTGACAGATA GACCTCCACC CATAATCTTG 
CAAGGACCAA TAAACCAAAC ACTTGCAGTA GACGGTACAG CATTGTTGAA GTGTAAAGCC 
ACTGGTGAGC CTCTGCCTGT AATTAGCTGG CTAAAGGAGG GCTTTACTTT TCTGGGGAGA 
GATCCAAGAG CCACGATCCA AGACCAAGGA ACACTGCAGA TTAAGAATTT ACGGATATCT 
GATACTGGCA CTTATACTTG TGTGGCTACA AGTTCCAGTG GAGAGACTTC CTGGAGTGCA 
GTGCTGGATG TAACAGAATC TGGAGCAACA ATCAGTAAAA ATTATGATAT GAATGACCTC 
CCGGGACCAC CATCCAAACC TCAGGTCACT GATGTTTCTA AGAACAGTGT CACCTTATCC 
TGGCAGCCAG GTACACCTGG CGTTCTTCCT GCAAGCGCGT ATATCATTGA GGCTTTCAGC 
CAATCGGTGA GCAATAGCTG GCAGACAGTG GCAAACCATG TTAAGACAAC TCTGTATACA 
GTAAGGGGGC TGAGGCCCAA CACAATCTAC TTGTTTATGG TCAGAGCGAT CAACCCACAA 
GGTCTCAGTG ATCCAAGTCC TATGTCGGAT CCTGTACGCA CACAAGATAT CAGCCCCCCA 
GCACAAGGAG TGGACCACAG ACAGGTGCAG AAGGAATTAG GTGATGTCGT TGTTCGTCTC 
CATAATCCAG TTGTCCTGAC ACCTACAACT GTTCAAGTCA CATGGACGGT GGACCGACAA 
CCCCAGTTTA TTCAGGGCTA CAGAGTGATG TACCGTCAGA CTTCGGGACT ACAAGCCTCA 
ACTGTGTGGC AGAATCTAGA CGCCAAAGTC CCGACTGAGA GGAGTGCTGT CCTTGTGAAT 
TTGAAAAAGG GGGTGACTTA TGAAATTAAA GTCCGGCCGT ATTTTAACGA GTTCCAAGGA 
ATGGACAGTG AATCGAAAAC AGTCCGAACC ACTGAGGAAG CCCCAAGTGC CCCTCCCCAG 
TCTGTCACTG TGCTGACAGT TGGAAGTCAC AACAGCACAA GCATCAGTGT TTCCTGGGAT 
CCTCCACCAG CCGACCACCA GAATGGAATT ATTCAGGAAT ATAAGATCTG GTGTCTGGGA 
AACGAAACGC GATTCCATAT CAATAAAACG GTGGATGCAG CCATTCGCTC TGTAGTAATA 
GGTGGCTTGT TCCCTGGAAT TCAGTACCGG GTAGAAGTGG CAGCTAGCAC AAGTGCAGGG 
GTTGGAGTAA AAAGTGAACC ACAGCCGATA ATAATTGGGG GACGTAACGA AGTTGTCATT 
ACTGAAAACA ATAACAGCAT CACTGAGCAA ATCACGGATG TCGTGAAGCA ACCGGCATTT 
ATAGCTGGCA TTGGTGGTGC CTGCTGGGTA ATTCTGATGG GTTTTAGCAT CTGGTTGTAC 
TGGAGAAGAA AGAAGAGAAA GGGACTCAGC AATTATGCTG TAACATTTCA AAGAGGAGAT 
GGAGGACTAA TGAGCAATGG GAGCCGTCCA GGTCTTCTAA ATGCTGGCGA TCCCAATTAC 
CCATGGCTTG CTGATTCTTG GCCAGCCACG AGTTTGCCAG TGAACAATAG CAATAGTGGC 
CCAAATGAAA TTGGAAATTT TGGGCGTGGA GATGTGCTGC CTCCGGTGCC AGGCCAAGGG 
GATAAAACAG CGACCATGCT CTCGGATGGA GCCATTTATA GCAGCATTGA CTTCACTACC 
AAAACCACTT ACAACAGTTC CAGCCAAATA ACACAGGCCA CCCCATATGC CACTACACAA 
ATCCTGCATT CAAACAGCAT CCACGAACTG GCAGTTGATC TTCCTGATCC ACAGTGGAAA 
AGCTCAGTTC AACAGAAGAC AGACCTCATG GGATTTGGTT ATTCGCTACC TGATCAGAAC 
AAGGGGAACA ACGCCTTACT TTACATCCCT GACTACCGAT TGGCTGAGGG ATTGTCTAAT 
AGAATGCCAC ACAACCAGTC ACAGGATTTC AGCACCACCA GCTCTCACAA CAGCTCAGAA 
AGGAGTGGCA GTCTCTCAGG TGGGAAAGGT GGAAAAAAGA AGAAAACTAA AAATTCTTCG 
AAAGCGCAGA AAAACAACGG ATCCACTTGG GCTAATGTCC CTCTACCTCC TCCTCCCGTC 
CAGCCCCTTC CTGGTACAGA GCTGGGCCAC TATGCTGCGG AACAAGAAAA TGGCTATGAC 
AGTGACAGCT GGTGCCCACC ATTACCGGTG CAAACATACT TGCATCAGGG TATGGAAGAT 
GAGCTGGAAG AAGACGAAGA TCGGGTCCCA ACACCTCCTG TTCGCGGCGT GGCCTCTTCA 
CCAGCTATCT CTTTTGGACA GCAGTCCACT GCCACTCTTA CTCCATCCCC ACGGGAAGAG 
ATGCAACCCA TGCTGCAAGC TCACTTGGAT GAGTTGACAA GGGCCTATCA GTTTGATATA 
GCAAAACAAA CATGGCACAT TCAAAGCAAT ACCCCACCTC CACAACCCCC AGCTCCGCCA 
TTAGGTTACG TGTCCGGAGC CTTGATTTCT GATTTGGAGA CAGATGTTCC AGATGAGGAT 
GCTGATGATG AAGAGGAACC ATTAGAAATT CCCAGGCCCC TCAGAGCACT AGACCAGACA 
CCTGGATCCA GTATGGACAA TCTAGACAGC TCTGTCACAG GTTCAATGGT AAATGGATGG 
GGCTCTGCAT CTGATGAGGA TCGTAACTTT TCTAGTCATA GATCTAGTGT AGGTAGCTCC 
TCAGATGGCT CCATCTTTGC CAGCGGCAGC TTTGCAAAAG CATTGGTGGC AGCAGCAGAC 
AAAGCTGGCT TTAGGCTGGA TGGAACCAGT CTTACGAGAA CAGGAAAAGC CTTTAGCTCC 
TCTCAAAGGC AGCGGCCCAC CAGCCCATTT TCTACGGACA GTAACACCAG TGCTGCCCAG 
AATCAAAGCC AGAGGCCTCG GCCCACGAAA AAACATAAGG GAGGACGGAT GGACCCACAG 
CCAGTGTTAC CTCATCGGAG GGAAGGGATG CCAGATGATC TTCCCCCACC ACCAGATCCC 
CCACCAGGTC AGGGTTTAAG GCAGCAGATA GGCCTGAGCC AACACTCTGG TAACGTGGAA 
AACTCAACAG AGAGAAAAGG AAGCTCTCTA GAGAGACAAC AAGCAGCCAA CTTAGAAGAC 
ACAAAGAGCT CATTGGATTG TCCAGCTAAA ACTGTTCTAG AGTGGCAGCG TCAAACCCAG 
GACTGGATAA ACTCTACAGA ACGCCAAGAA GAAACTCGGA AAGCCCCACA CAAACAAGGG 
GTTGGATCAG AGGAGTCCCT GGTGCCCTAC AGCAAGCCCA GCTTCCCGTC TCCTGGCGGG 
CACAGCTCAT CAGGAACATC TTCCTCTAAA GGGTCCACTG GCCCTCGGAA GGCTGACGTG 
CTGAGAGGCA GCCACCAGAG GAATGCCAAT GACCTTCTCG ACATTGGATA TGTGGGCTCA 
AATAGTCAAG GACAGTTTAC AGGTGAATTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo2 ( XM_030249139.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030104999.1 Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X5, mRNA.

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CloneID	OMu52758
Clone ID Related Accession (Same CDS sequence)	XM_006523038.3 , XM_006523038.4 , XM_030249140.1 , XM_030249139.1
Accession Version	XM_030249139.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4773bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	roundabout homolog 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGAA GACAAGAAAG TATCTGTGGA AGGCCGTGGA CGTGGACGCC AGGACTACTG 
ATGCTGATCA TCCTTGGCAT TCATCAGGGG AGTGGGCAAG GTCAAGGATC TCGTCTTCGT 
CAAGAAGACT TTCCCCCCAG GATCGTAGAA CACCCTTCTG ATGTCATCGT CTCCAAGGGA 
GAGCCCACCA CTCTGAACTG TAAAGCAGAG GGCCGACCCA CCCCCACCAT TGAATGGTAC 
AAGGATGGTG AGAGGGTGGA GACAGACAAG GATGATCCCA GGTCCCACAG AATGCTTCTG 
CCCAGCGGAT CTTTATTCTT TTTGCGAATT GTTCATGGGC GCAGAAGTAA ACCGGACGAA 
GGGAGTTACG TTTGTGTTGC AAGGAACTAT CTTGGTGAAG CAGTGAGTCG AAATGCATCT 
CTGGAAGTGG CATTATTGCG AGATGACTTC CGGCAAAACC CCACAGATGT GGTAGTCGCA 
GCTGGAGAGC CTGCAATCTT GGAGTGCCAG CCACCACGGG GACACCCAGA ACCAACCATC 
TACTGGAAAA AGGACAAAGT CCGAATTGAT GACAAGGAAG AGAGAATAAG TATCCGTGGT 
GGGAAGCTGA TGATCTCTAA TACTAGGAAA AGCGATGCTG GCATGTACAC CTGTGTGGGA 
ACCAATATGG TGGGAGAAAG GGACAGCGAC CCTGCAGAGC TCACTGTCTT TGAACGACCC 
ACATTTCTCA GGAGGCCAAT TAACCAGGTG GTGCTAGAGG AAGAAGCTGT AGAATTCCGT 
TGTCAGGTCC AAGGAGATCC CCAGCCAACG GTGAGGTGGA AAAAAGATGA TGCAGACTTG 
CCGAGAGGAA GGTATGATAT CAAAGATGAC TACACGCTGA GAATTAAAAA GGCCATGAGT 
ACTGATGAAG GTACCTATGT GTGTATTGCT GAGAATCGGG TGGGAAAAGT GGAAGCCTCT 
GCTACCCTCA CTGTCCGAGT TCGCCCTGTT GCTCCTCCAC AGTTTGTGGT TAGGCCAAGA 
GATCAGATCG TTGCTCAAGG CCGAACAGTG ACATTCCCCT GTGAAACTAA AGGAAACCCA 
CAGCCAGCTG TTTTTTGGCA GAAAGAAGGC AGCCAGAACC TACTTTTCCC GAATCAACCT 
CAGCAGCCCA ACAGCCGATG TTCAGTGTCG CCCACGGGGG ACCTCACCAT CACCAACATC 
CAGCGTTCAG ATGCGGGTTA CTACATCTGC CAGGCCCTAA CCGTGGCAGG AAGCATTTTA 
GCTAAAGCAC AGTTGGAAGT TACTGACGTT TTGACAGATA GACCTCCACC CATAATCTTG 
CAAGGACCAA TAAACCAAAC ACTTGCAGTA GACGGTACAG CATTGTTGAA GTGTAAAGCC 
ACTGGTGAGC CTCTGCCTGT AATTAGCTGG CTAAAGGAGG GCTTTACTTT TCTGGGGAGA 
GATCCAAGAG CCACGATCCA AGACCAAGGA ACACTGCAGA TTAAGAATTT ACGGATATCT 
GATACTGGCA CTTATACTTG TGTGGCTACA AGTTCCAGTG GAGAGACTTC CTGGAGTGCA 
GTGCTGGATG TAACAGAATC TGGAGCAACA ATCAGTAAAA ATTATGATAT GAATGACCTC 
CCGGGACCAC CATCCAAACC TCAGGTCACT GATGTTTCTA AGAACAGTGT CACCTTATCC 
TGGCAGCCAG GTACACCTGG CGTTCTTCCT GCAAGCGCGT ATATCATTGA GGCTTTCAGC 
CAATCGGTGA GCAATAGCTG GCAGACAGTG GCAAACCATG TTAAGACAAC TCTGTATACA 
GTAAGGGGGC TGAGGCCCAA CACAATCTAC TTGTTTATGG TCAGAGCGAT CAACCCACAA 
GGTCTCAGTG ATCCAAGTCC TATGTCGGAT CCTGTACGCA CACAAGATAT CAGCCCCCCA 
GCACAAGGAG TGGACCACAG ACAGGTGCAG AAGGAATTAG GTGATGTCGT TGTTCGTCTC 
CATAATCCAG TTGTCCTGAC ACCTACAACT GTTCAAGTCA CATGGACGGT GGACCGACAA 
CCCCAGTTTA TTCAGGGCTA CAGAGTGATG TACCGTCAGA CTTCGGGACT ACAAGCCTCA 
ACTGTGTGGC AGAATCTAGA CGCCAAAGTC CCGACTGAGA GGAGTGCTGT CCTTGTGAAT 
TTGAAAAAGG GGGTGACTTA TGAAATTAAA GTCCGGCCGT ATTTTAACGA GTTCCAAGGA 
ATGGACAGTG AATCGAAAAC AGTCCGAACC ACTGAGGAAG CCCCAAGTGC CCCTCCCCAG 
TCTGTCACTG TGCTGACAGT TGGAAGTCAC AACAGCACAA GCATCAGTGT TTCCTGGGAT 
CCTCCACCAG CCGACCACCA GAATGGAATT ATTCAGGAAT ATAAGATCTG GTGTCTGGGA 
AACGAAACGC GATTCCATAT CAATAAAACG GTGGATGCAG CCATTCGCTC TGTAGTAATA 
GGTGGCTTGT TCCCTGGAAT TCAGTACCGG GTAGAAGTGG CAGCTAGCAC AAGTGCAGGG 
GTTGGAGTAA AAAGTGAACC ACAGCCGATA ATAATTGGGG GACGTAACGA AGTTGTCATT 
ACTGAAAACA ATAACAGCAT CACTGAGCAA ATCACGGATG TCGTGAAGCA ACCGGCATTT 
ATAGCTGGCA TTGGTGGTGC CTGCTGGGTA ATTCTGATGG GTTTTAGCAT CTGGTTGTAC 
TGGAGAAGAA AGAAGAGAAA GGGACTCAGC AATTATGCTG TAACATTTCA AAGAGGAGAT 
GGAGGACTAA TGAGCAATGG GAGCCGTCCA GGTCTTCTAA ATGCTGGCGA TCCCAATTAC 
CCATGGCTTG CTGATTCTTG GCCAGCCACG AGTTTGCCAG TGAACAATAG CAATAGTGGC 
CCAAATGAAA TTGGAAATTT TGGGCGTGGA GATGTGCTGC CTCCGGTGCC AGGCCAAGGG 
GATAAAACAG CGACCATGCT CTCGGATGGA GCCATTTATA GCAGCATTGA CTTCACTACC 
AAAACCACTT ACAACAGTTC CAGCCAAATA ACACAGGCCA CCCCATATGC CACTACACAA 
ATCCTGCATT CAAACAGCAT CCACGAACTG GCAGTTGATC TTCCTGATCC ACAGTGGAAA 
AGCTCAGTTC AACAGAAGAC AGACCTCATG GGATTTGGTT ATTCGCTACC TGATCAGAAC 
AAGGGGAACA ACGCCTTACT TTACATCCCT GACTACCGAT TGGCTGAGGG ATTGTCTAAT 
AGAATGCCAC ACAACCAGTC ACAGGATTTC AGCACCACCA GCTCTCACAA CAGCTCAGAA 
AGGAGTGGCA GTCTCTCAGG TGGGAAAGGT GGAAAAAAGA AGAAAACTAA AAATTCTTCG 
AAAGCGCAGA AAAACAACGG ATCCACTTGG GCTAATGTCC CTCTACCTCC TCCTCCCGTC 
CAGCCCCTTC CTGGTACAGA GCTGGGCCAC TATGCTGCGG AACAAGAAAA TGGCTATGAC 
AGTGACAGCT GGTGCCCACC ATTACCGGTG CAAACATACT TGCATCAGGG TATGGAAGAT 
GAGCTGGAAG AAGACGAAGA TCGGGTCCCA ACACCTCCTG TTCGCGGCGT GGCCTCTTCA 
CCAGCTATCT CTTTTGGACA GCAGTCCACT GCCACTCTTA CTCCATCCCC ACGGGAAGAG 
ATGCAACCCA TGCTGCAAGC TCACTTGGAT GAGTTGACAA GGGCCTATCA GTTTGATATA 
GCAAAACAAA CATGGCACAT TCAAAGCAAT ACCCCACCTC CACAACCCCC AGCTCCGCCA 
TTAGGTTACG TGTCCGGAGC CTTGATTTCT GATTTGGAGA CAGATGTTCC AGATGAGGAT 
GCTGATGATG AAGAGGAACC ATTAGAAATT CCCAGGCCCC TCAGAGCACT AGACCAGACA 
CCTGGATCCA GTATGGACAA TCTAGACAGC TCTGTCACAG GTTCAATGGT AAATGGATGG 
GGCTCTGCAT CTGATGAGGA TCGTAACTTT TCTAGTCATA GATCTAGTGT AGGTAGCTCC 
TCAGATGGCT CCATCTTTGC CAGCGGCAGC TTTGCAAAAG CATTGGTGGC AGCAGCAGAC 
AAAGCTGGCT TTAGGCTGGA TGGAACCAGT CTTACGAGAA CAGGAAAAGC CTTTAGCTCC 
TCTCAAAGGC AGCGGCCCAC CAGCCCATTT TCTACGGACA GTAACACCAG TGCTGCCCAG 
AATCAAAGCC AGAGGCCTCG GCCCACGAAA AAACATAAGG GAGGACGGAT GGACCCACAG 
CCAGTGTTAC CTCATCGGAG GGAAGGGATG CCAGATGATC TTCCCCCACC ACCAGATCCC 
CCACCAGGTC AGGGTTTAAG GCAGCAGATA GGCCTGAGCC AACACTCTGG TAACGTGGAA 
AACTCAACAG AGAGAAAAGG AAGCTCTCTA GAGAGACAAC AAGCAGCCAA CTTAGAAGAC 
ACAAAGAGCT CATTGGATTG TCCAGCTAAA ACTGTTCTAG AGTGGCAGCG TCAAACCCAG 
GACTGGATAA ACTCTACAGA ACGCCAAGAA GAAACTCGGA AAGCCCCACA CAAACAAGGG 
GTTGGATCAG AGGAGTCCCT GGTGCCCTAC AGCAAGCCCA GCTTCCCGTC TCCTGGCGGG 
CACAGCTCAT CAGGAACATC TTCCTCTAAA GGGTCCACTG GCCCTCGGAA GGCTGACGTG 
CTGAGAGGCA GCCACCAGAG GAATGCCAAT GACCTTCTCG ACATTGGATA TGTGGGCTCA 
AATAGTCAAG GACAGTTTAC AGGTGAATTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030104999.1
CDS	233..5005
Translation	MARRQESICGRPWTWTPGLLMLIILGIHQGSGQGQGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKAMSTDEGTYVCIAENRVGKVEASATLTVRVRPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPINQTLAVDGTALLKCKATGEPLPVISWLKEGFTFLGRDPRATIQDQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDMNDLPGPPSKPQVTDVSKNSVTLSWQPGTPGVLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVVVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQASTVWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSHNSTSISVSWDPPPADHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVVIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGGRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPNYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTTYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSVQQKTDLMGFGYSLPDQNKGNNALLYIPDYRLAEGLSNRMPHNQSQDFSTTSSHNSSERSGSLSGGKGGKKKKTKNSSKAQKNNGSTWANVPLPPPPVQPLPGTELGHYAAEQENGYDSDSWCPPLPVQTYLHQGMEDELEEDEDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNTPPPQPPAPPLGYVSGALISDLETDVPDEDADDEEEPLEIPRPLRALDQTPGSSMDNLDSSVTGSMVNGWGSASDEDRNFSSHRSSVGSSSDGSIFASGSFAKALVAAADKAGFRLDGTSLTRTGKAFSSSQRQRPTSPFSTDSNTSAAQNQSQRPRPTKKHKGGRMDPQPVLPHRREGMPDDLPPPPDPPPGQGLRQQIGLSQHSGNVENSTERKGSSLERQQAANLEDTKSSLDCPAKTVLEWQRQTQDWINSTERQEETRKAPHKQGVGSEESLVPYSKPSFPSPGGHSSSGTSSSKGSTGPRKADVLRGSHQRNANDLLDIGYVGSNSQGQFTGEL

Target ORF information:

RefSeq Version	XM_030249139.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249139.1

ORF Insert Sequence:

ATGGCCAGAA GACAAGAAAG TATCTGTGGA AGGCCGTGGA CGTGGACGCC AGGACTACTG 
ATGCTGATCA TCCTTGGCAT TCATCAGGGG AGTGGGCAAG GTCAAGGATC TCGTCTTCGT 
CAAGAAGACT TTCCCCCCAG GATCGTAGAA CACCCTTCTG ATGTCATCGT CTCCAAGGGA 
GAGCCCACCA CTCTGAACTG TAAAGCAGAG GGCCGACCCA CCCCCACCAT TGAATGGTAC 
AAGGATGGTG AGAGGGTGGA GACAGACAAG GATGATCCCA GGTCCCACAG AATGCTTCTG 
CCCAGCGGAT CTTTATTCTT TTTGCGAATT GTTCATGGGC GCAGAAGTAA ACCGGACGAA 
GGGAGTTACG TTTGTGTTGC AAGGAACTAT CTTGGTGAAG CAGTGAGTCG AAATGCATCT 
CTGGAAGTGG CATTATTGCG AGATGACTTC CGGCAAAACC CCACAGATGT GGTAGTCGCA 
GCTGGAGAGC CTGCAATCTT GGAGTGCCAG CCACCACGGG GACACCCAGA ACCAACCATC 
TACTGGAAAA AGGACAAAGT CCGAATTGAT GACAAGGAAG AGAGAATAAG TATCCGTGGT 
GGGAAGCTGA TGATCTCTAA TACTAGGAAA AGCGATGCTG GCATGTACAC CTGTGTGGGA 
ACCAATATGG TGGGAGAAAG GGACAGCGAC CCTGCAGAGC TCACTGTCTT TGAACGACCC 
ACATTTCTCA GGAGGCCAAT TAACCAGGTG GTGCTAGAGG AAGAAGCTGT AGAATTCCGT 
TGTCAGGTCC AAGGAGATCC CCAGCCAACG GTGAGGTGGA AAAAAGATGA TGCAGACTTG 
CCGAGAGGAA GGTATGATAT CAAAGATGAC TACACGCTGA GAATTAAAAA GGCCATGAGT 
ACTGATGAAG GTACCTATGT GTGTATTGCT GAGAATCGGG TGGGAAAAGT GGAAGCCTCT 
GCTACCCTCA CTGTCCGAGT TCGCCCTGTT GCTCCTCCAC AGTTTGTGGT TAGGCCAAGA 
GATCAGATCG TTGCTCAAGG CCGAACAGTG ACATTCCCCT GTGAAACTAA AGGAAACCCA 
CAGCCAGCTG TTTTTTGGCA GAAAGAAGGC AGCCAGAACC TACTTTTCCC GAATCAACCT 
CAGCAGCCCA ACAGCCGATG TTCAGTGTCG CCCACGGGGG ACCTCACCAT CACCAACATC 
CAGCGTTCAG ATGCGGGTTA CTACATCTGC CAGGCCCTAA CCGTGGCAGG AAGCATTTTA 
GCTAAAGCAC AGTTGGAAGT TACTGACGTT TTGACAGATA GACCTCCACC CATAATCTTG 
CAAGGACCAA TAAACCAAAC ACTTGCAGTA GACGGTACAG CATTGTTGAA GTGTAAAGCC 
ACTGGTGAGC CTCTGCCTGT AATTAGCTGG CTAAAGGAGG GCTTTACTTT TCTGGGGAGA 
GATCCAAGAG CCACGATCCA AGACCAAGGA ACACTGCAGA TTAAGAATTT ACGGATATCT 
GATACTGGCA CTTATACTTG TGTGGCTACA AGTTCCAGTG GAGAGACTTC CTGGAGTGCA 
GTGCTGGATG TAACAGAATC TGGAGCAACA ATCAGTAAAA ATTATGATAT GAATGACCTC 
CCGGGACCAC CATCCAAACC TCAGGTCACT GATGTTTCTA AGAACAGTGT CACCTTATCC 
TGGCAGCCAG GTACACCTGG CGTTCTTCCT GCAAGCGCGT ATATCATTGA GGCTTTCAGC 
CAATCGGTGA GCAATAGCTG GCAGACAGTG GCAAACCATG TTAAGACAAC TCTGTATACA 
GTAAGGGGGC TGAGGCCCAA CACAATCTAC TTGTTTATGG TCAGAGCGAT CAACCCACAA 
GGTCTCAGTG ATCCAAGTCC TATGTCGGAT CCTGTACGCA CACAAGATAT CAGCCCCCCA 
GCACAAGGAG TGGACCACAG ACAGGTGCAG AAGGAATTAG GTGATGTCGT TGTTCGTCTC 
CATAATCCAG TTGTCCTGAC ACCTACAACT GTTCAAGTCA CATGGACGGT GGACCGACAA 
CCCCAGTTTA TTCAGGGCTA CAGAGTGATG TACCGTCAGA CTTCGGGACT ACAAGCCTCA 
ACTGTGTGGC AGAATCTAGA CGCCAAAGTC CCGACTGAGA GGAGTGCTGT CCTTGTGAAT 
TTGAAAAAGG GGGTGACTTA TGAAATTAAA GTCCGGCCGT ATTTTAACGA GTTCCAAGGA 
ATGGACAGTG AATCGAAAAC AGTCCGAACC ACTGAGGAAG CCCCAAGTGC CCCTCCCCAG 
TCTGTCACTG TGCTGACAGT TGGAAGTCAC AACAGCACAA GCATCAGTGT TTCCTGGGAT 
CCTCCACCAG CCGACCACCA GAATGGAATT ATTCAGGAAT ATAAGATCTG GTGTCTGGGA 
AACGAAACGC GATTCCATAT CAATAAAACG GTGGATGCAG CCATTCGCTC TGTAGTAATA 
GGTGGCTTGT TCCCTGGAAT TCAGTACCGG GTAGAAGTGG CAGCTAGCAC AAGTGCAGGG 
GTTGGAGTAA AAAGTGAACC ACAGCCGATA ATAATTGGGG GACGTAACGA AGTTGTCATT 
ACTGAAAACA ATAACAGCAT CACTGAGCAA ATCACGGATG TCGTGAAGCA ACCGGCATTT 
ATAGCTGGCA TTGGTGGTGC CTGCTGGGTA ATTCTGATGG GTTTTAGCAT CTGGTTGTAC 
TGGAGAAGAA AGAAGAGAAA GGGACTCAGC AATTATGCTG TAACATTTCA AAGAGGAGAT 
GGAGGACTAA TGAGCAATGG GAGCCGTCCA GGTCTTCTAA ATGCTGGCGA TCCCAATTAC 
CCATGGCTTG CTGATTCTTG GCCAGCCACG AGTTTGCCAG TGAACAATAG CAATAGTGGC 
CCAAATGAAA TTGGAAATTT TGGGCGTGGA GATGTGCTGC CTCCGGTGCC AGGCCAAGGG 
GATAAAACAG CGACCATGCT CTCGGATGGA GCCATTTATA GCAGCATTGA CTTCACTACC 
AAAACCACTT ACAACAGTTC CAGCCAAATA ACACAGGCCA CCCCATATGC CACTACACAA 
ATCCTGCATT CAAACAGCAT CCACGAACTG GCAGTTGATC TTCCTGATCC ACAGTGGAAA 
AGCTCAGTTC AACAGAAGAC AGACCTCATG GGATTTGGTT ATTCGCTACC TGATCAGAAC 
AAGGGGAACA ACGCCTTACT TTACATCCCT GACTACCGAT TGGCTGAGGG ATTGTCTAAT 
AGAATGCCAC ACAACCAGTC ACAGGATTTC AGCACCACCA GCTCTCACAA CAGCTCAGAA 
AGGAGTGGCA GTCTCTCAGG TGGGAAAGGT GGAAAAAAGA AGAAAACTAA AAATTCTTCG 
AAAGCGCAGA AAAACAACGG ATCCACTTGG GCTAATGTCC CTCTACCTCC TCCTCCCGTC 
CAGCCCCTTC CTGGTACAGA GCTGGGCCAC TATGCTGCGG AACAAGAAAA TGGCTATGAC 
AGTGACAGCT GGTGCCCACC ATTACCGGTG CAAACATACT TGCATCAGGG TATGGAAGAT 
GAGCTGGAAG AAGACGAAGA TCGGGTCCCA ACACCTCCTG TTCGCGGCGT GGCCTCTTCA 
CCAGCTATCT CTTTTGGACA GCAGTCCACT GCCACTCTTA CTCCATCCCC ACGGGAAGAG 
ATGCAACCCA TGCTGCAAGC TCACTTGGAT GAGTTGACAA GGGCCTATCA GTTTGATATA 
GCAAAACAAA CATGGCACAT TCAAAGCAAT ACCCCACCTC CACAACCCCC AGCTCCGCCA 
TTAGGTTACG TGTCCGGAGC CTTGATTTCT GATTTGGAGA CAGATGTTCC AGATGAGGAT 
GCTGATGATG AAGAGGAACC ATTAGAAATT CCCAGGCCCC TCAGAGCACT AGACCAGACA 
CCTGGATCCA GTATGGACAA TCTAGACAGC TCTGTCACAG GTTCAATGGT AAATGGATGG 
GGCTCTGCAT CTGATGAGGA TCGTAACTTT TCTAGTCATA GATCTAGTGT AGGTAGCTCC 
TCAGATGGCT CCATCTTTGC CAGCGGCAGC TTTGCAAAAG CATTGGTGGC AGCAGCAGAC 
AAAGCTGGCT TTAGGCTGGA TGGAACCAGT CTTACGAGAA CAGGAAAAGC CTTTAGCTCC 
TCTCAAAGGC AGCGGCCCAC CAGCCCATTT TCTACGGACA GTAACACCAG TGCTGCCCAG 
AATCAAAGCC AGAGGCCTCG GCCCACGAAA AAACATAAGG GAGGACGGAT GGACCCACAG 
CCAGTGTTAC CTCATCGGAG GGAAGGGATG CCAGATGATC TTCCCCCACC ACCAGATCCC 
CCACCAGGTC AGGGTTTAAG GCAGCAGATA GGCCTGAGCC AACACTCTGG TAACGTGGAA 
AACTCAACAG AGAGAAAAGG AAGCTCTCTA GAGAGACAAC AAGCAGCCAA CTTAGAAGAC 
ACAAAGAGCT CATTGGATTG TCCAGCTAAA ACTGTTCTAG AGTGGCAGCG TCAAACCCAG 
GACTGGATAA ACTCTACAGA ACGCCAAGAA GAAACTCGGA AAGCCCCACA CAAACAAGGG 
GTTGGATCAG AGGAGTCCCT GGTGCCCTAC AGCAAGCCCA GCTTCCCGTC TCCTGGCGGG 
CACAGCTCAT CAGGAACATC TTCCTCTAAA GGGTCCACTG GCCCTCGGAA GGCTGACGTG 
CTGAGAGGCA GCCACCAGAG GAATGCCAAT GACCTTCTCG ACATTGGATA TGTGGGCTCA 
AATAGTCAAG GACAGTTTAC AGGTGAATTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo2 ( XM_006523039.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523102.1 Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X7, mRNA.

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CloneID	OMu52759
Clone ID Related Accession (Same CDS sequence)	XM_006523039.4 , XM_006523039.3
Accession Version	XM_006523039.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4728bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	roundabout homolog 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006523039.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCCTC TGATGTTTAC ACTATTATTG CTCTTTGGAT TTCTCTGCAT TCAGATTGAT 
GGATCTCGTC TTCGTCAAGA AGACTTTCCC CCCAGGATCG TAGAACACCC TTCTGATGTC 
ATCGTCTCCA AGGGAGAGCC CACCACTCTG AACTGTAAAG CAGAGGGCCG ACCCACCCCC 
ACCATTGAAT GGTACAAGGA TGGTGAGAGG GTGGAGACAG ACAAGGATGA TCCCAGGTCC 
CACAGAATGC TTCTGCCCAG CGGATCTTTA TTCTTTTTGC GAATTGTTCA TGGGCGCAGA 
AGTAAACCGG ACGAAGGGAG TTACGTTTGT GTTGCAAGGA ACTATCTTGG TGAAGCAGTG 
AGTCGAAATG CATCTCTGGA AGTGGCATTA TTGCGAGATG ACTTCCGGCA AAACCCCACA 
GATGTGGTAG TCGCAGCTGG AGAGCCTGCA ATCTTGGAGT GCCAGCCACC ACGGGGACAC 
CCAGAACCAA CCATCTACTG GAAAAAGGAC AAAGTCCGAA TTGATGACAA GGAAGAGAGA 
ATAAGTATCC GTGGTGGGAA GCTGATGATC TCTAATACTA GGAAAAGCGA TGCTGGCATG 
TACACCTGTG TGGGAACCAA TATGGTGGGA GAAAGGGACA GCGACCCTGC AGAGCTCACT 
GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTGCT AGAGGAAGAA 
GCTGTAGAAT TCCGTTGTCA GGTCCAAGGA GATCCCCAGC CAACGGTGAG GTGGAAAAAA 
GATGATGCAG ACTTGCCGAG AGGAAGGTAT GATATCAAAG ATGACTACAC GCTGAGAATT 
AAAAAGGCCA TGAGTACTGA TGAAGGTACC TATGTGTGTA TTGCTGAGAA TCGGGTGGGA 
AAAGTGGAAG CCTCTGCTAC CCTCACTGTC CGAGTTCGCC CTGTTGCTCC TCCACAGTTT 
GTGGTTAGGC CAAGAGATCA GATCGTTGCT CAAGGCCGAA CAGTGACATT CCCCTGTGAA 
ACTAAAGGAA ACCCACAGCC AGCTGTTTTT TGGCAGAAAG AAGGCAGCCA GAACCTACTT 
TTCCCGAATC AACCTCAGCA GCCCAACAGC CGATGTTCAG TGTCGCCCAC GGGGGACCTC 
ACCATCACCA ACATCCAGCG TTCAGATGCG GGTTACTACA TCTGCCAGGC CCTAACCGTG 
GCAGGAAGCA TTTTAGCTAA AGCACAGTTG GAAGTTACTG ACGTTTTGAC AGATAGACCT 
CCACCCATAA TCTTGCAAGG ACCAATAAAC CAAACACTTG CAGTAGACGG TACAGCATTG 
TTGAAGTGTA AAGCCACTGG TGAGCCTCTG CCTGTAATTA GCTGGCTAAA GGAGGGCTTT 
ACTTTTCTGG GGAGAGATCC AAGAGCCACG ATCCAAGACC AAGGAACACT GCAGATTAAG 
AATTTACGGA TATCTGATAC TGGCACTTAT ACTTGTGTGG CTACAAGTTC CAGTGGAGAG 
ACTTCCTGGA GTGCAGTGCT GGATGTAACA GAATCTGGAG CAACAATCAG TAAAAATTAT 
GATATGAATG ACCTCCCGGG ACCACCATCC AAACCTCAGG TCACTGATGT TTCTAAGAAC 
AGTGTCACCT TATCCTGGCA GCCAGGTACA CCTGGCGTTC TTCCTGCAAG CGCGTATATC 
ATTGAGGCTT TCAGCCAATC GGTGAGCAAT AGCTGGCAGA CAGTGGCAAA CCATGTTAAG 
ACAACTCTGT ATACAGTAAG GGGGCTGAGG CCCAACACAA TCTACTTGTT TATGGTCAGA 
GCGATCAACC CACAAGGTCT CAGTGATCCA AGTCCTATGT CGGATCCTGT ACGCACACAA 
GATATCAGCC CCCCAGCACA AGGAGTGGAC CACAGACAGG TGCAGAAGGA ATTAGGTGAT 
GTCGTTGTTC GTCTCCATAA TCCAGTTGTC CTGACACCTA CAACTGTTCA AGTCACATGG 
ACGGTGGACC GACAACCCCA GTTTATTCAG GGCTACAGAG TGATGTACCG TCAGACTTCG 
GGACTACAAG CCTCAACTGT GTGGCAGAAT CTAGACGCCA AAGTCCCGAC TGAGAGGAGT 
GCTGTCCTTG TGAATTTGAA AAAGGGGGTG ACTTATGAAA TTAAAGTCCG GCCGTATTTT 
AACGAGTTCC AAGGAATGGA CAGTGAATCG AAAACAGTCC GAACCACTGA GGAAGCCCCA 
AGTGCCCCTC CCCAGTCTGT CACTGTGCTG ACAGTTGGAA GTCACAACAG CACAAGCATC 
AGTGTTTCCT GGGATCCTCC ACCAGCCGAC CACCAGAATG GAATTATTCA GGAATATAAG 
ATCTGGTGTC TGGGAAACGA AACGCGATTC CATATCAATA AAACGGTGGA TGCAGCCATT 
CGCTCTGTAG TAATAGGTGG CTTGTTCCCT GGAATTCAGT ACCGGGTAGA AGTGGCAGCT 
AGCACAAGTG CAGGGGTTGG AGTAAAAAGT GAACCACAGC CGATAATAAT TGGGGGACGT 
AACGAAGTTG TCATTACTGA AAACAATAAC AGCATCACTG AGCAAATCAC GGATGTCGTG 
AAGCAACCGG CATTTATAGC TGGCATTGGT GGTGCCTGCT GGGTAATTCT GATGGGTTTT 
AGCATCTGGT TGTACTGGAG AAGAAAGAAG AGAAAGGGAC TCAGCAATTA TGCTGTAACA 
TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGGAGCC GTCCAGGTCT TCTAAATGCT 
GGCGATCCCA ATTACCCATG GCTTGCTGAT TCTTGGCCAG CCACGAGTTT GCCAGTGAAC 
AATAGCAATA GTGGCCCAAA TGAAATTGGA AATTTTGGGC GTGGAGATGT GCTGCCTCCG 
GTGCCAGGCC AAGGGGATAA AACAGCGACC ATGCTCTCGG ATGGAGCCAT TTATAGCAGC 
ATTGACTTCA CTACCAAAAC CACTTACAAC AGTTCCAGCC AAATAACACA GGCCACCCCA 
TATGCCACTA CACAAATCCT GCATTCAAAC AGCATCCACG AACTGGCAGT TGATCTTCCT 
GATCCACAGT GGAAAAGCTC AGTTCAACAG AAGACAGACC TCATGGGATT TGGTTATTCG 
CTACCTGATC AGAACAAGGG GAACAACGCC TTACTTTACA TCCCTGACTA CCGATTGGCT 
GAGGGATTGT CTAATAGAAT GCCACACAAC CAGTCACAGG ATTTCAGCAC CACCAGCTCT 
CACAACAGCT CAGAAAGGAG TGGCAGTCTC TCAGGTGGGA AAGGTGGAAA AAAGAAGAAA 
ACTAAAAATT CTTCGAAAGC GCAGAAAAAC AACGGATCCA CTTGGGCTAA TGTCCCTCTA 
CCTCCTCCTC CCGTCCAGCC CCTTCCTGGT ACAGAGCTGG GCCACTATGC TGCGGAACAA 
GAAAATGGCT ATGACAGTGA CAGCTGGTGC CCACCATTAC CGGTGCAAAC ATACTTGCAT 
CAGGGTATGG AAGATGAGCT GGAAGAAGAC GAAGATCGGG TCCCAACACC TCCTGTTCGC 
GGCGTGGCCT CTTCACCAGC TATCTCTTTT GGACAGCAGT CCACTGCCAC TCTTACTCCA 
TCCCCACGGG AAGAGATGCA ACCCATGCTG CAAGCTCACT TGGATGAGTT GACAAGGGCC 
TATCAGTTTG ATATAGCAAA ACAAACATGG CACATTCAAA GCAATACCCC ACCTCCACAA 
CCCCCAGCTC CGCCATTAGG TTACGTGTCC GGAGCCTTGA TTTCTGATTT GGAGACAGAT 
GTTCCAGATG AGGATGCTGA TGATGAAGAG GAACCATTAG AAATTCCCAG GCCCCTCAGA 
GCACTAGACC AGACACCTGG ATCCAGTATG GACAATCTAG ACAGCTCTGT CACAGGTTCA 
ATGGTAAATG GATGGGGCTC TGCATCTGAT GAGGATCGTA ACTTTTCTAG TCATAGATCT 
AGTGTAGGTA GCTCCTCAGA TGGCTCCATC TTTGCCAGCG GCAGCTTTGC AAAAGCATTG 
GTGGCAGCAG CAGACAAAGC TGGCTTTAGG CTGGATGGAA CCAGTCTTAC GAGAACAGGA 
AAAGCCTTTA GCTCCTCTCA AAGGCAGCGG CCCACCAGCC CATTTTCTAC GGACAGTAAC 
ACCAGTGCTG CCCAGAATCA AAGCCAGAGG CCTCGGCCCA CGAAAAAACA TAAGGGAGGA 
CGGATGGACC CACAGCCAGT GTTACCTCAT CGGAGGGAAG GGATGCCAGA TGATCTTCCC 
CCACCACCAG ATCCCCCACC AGGTCAGGGT TTAAGGCAGC AGATAGGCCT GAGCCAACAC 
TCTGGTAACG TGGAAAACTC AACAGAGAGA AAAGGAAGCT CTCTAGAGAG ACAACAAGCA 
GCCAACTTAG AAGACACAAA GAGCTCATTG GATTGTCCAG CTAAAACTGT TCTAGAGTGG 
CAGCGTCAAA CCCAGGACTG GATAAACTCT ACAGAACGCC AAGAAGAAAC TCGGAAAGCC 
CCACACAAAC AAGGGGTTGG ATCAGAGGAG TCCCTGGTGC CCTACAGCAA GCCCAGCTTC 
CCGTCTCCTG GCGGGCACAG CTCATCAGGA ACATCTTCCT CTAAAGGGTC CACTGGCCCT 
CGGAAGGCTG ACGTGCTGAG AGGCAGCCAC CAGAGGAATG CCAATGACCT TCTCGACATT 
GGATATGTGG GCTCAAATAG TCAAGGACAG TTTACAGGTG AATTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523102.1
CDS	2416..7143
Translation	MNPLMFTLLLLFGFLCIQIDGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKAMSTDEGTYVCIAENRVGKVEASATLTVRVRPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPINQTLAVDGTALLKCKATGEPLPVISWLKEGFTFLGRDPRATIQDQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDMNDLPGPPSKPQVTDVSKNSVTLSWQPGTPGVLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVVVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQASTVWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSHNSTSISVSWDPPPADHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVVIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGGRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPNYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTTYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSVQQKTDLMGFGYSLPDQNKGNNALLYIPDYRLAEGLSNRMPHNQSQDFSTTSSHNSSERSGSLSGGKGGKKKKTKNSSKAQKNNGSTWANVPLPPPPVQPLPGTELGHYAAEQENGYDSDSWCPPLPVQTYLHQGMEDELEEDEDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNTPPPQPPAPPLGYVSGALISDLETDVPDEDADDEEEPLEIPRPLRALDQTPGSSMDNLDSSVTGSMVNGWGSASDEDRNFSSHRSSVGSSSDGSIFASGSFAKALVAAADKAGFRLDGTSLTRTGKAFSSSQRQRPTSPFSTDSNTSAAQNQSQRPRPTKKHKGGRMDPQPVLPHRREGMPDDLPPPPDPPPGQGLRQQIGLSQHSGNVENSTERKGSSLERQQAANLEDTKSSLDCPAKTVLEWQRQTQDWINSTERQEETRKAPHKQGVGSEESLVPYSKPSFPSPGGHSSSGTSSSKGSTGPRKADVLRGSHQRNANDLLDIGYVGSNSQGQFTGEL

Target ORF information:

RefSeq Version	XM_006523039.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523039.4

ORF Insert Sequence:

ATGAATCCTC TGATGTTTAC ACTATTATTG CTCTTTGGAT TTCTCTGCAT TCAGATTGAT 
GGATCTCGTC TTCGTCAAGA AGACTTTCCC CCCAGGATCG TAGAACACCC TTCTGATGTC 
ATCGTCTCCA AGGGAGAGCC CACCACTCTG AACTGTAAAG CAGAGGGCCG ACCCACCCCC 
ACCATTGAAT GGTACAAGGA TGGTGAGAGG GTGGAGACAG ACAAGGATGA TCCCAGGTCC 
CACAGAATGC TTCTGCCCAG CGGATCTTTA TTCTTTTTGC GAATTGTTCA TGGGCGCAGA 
AGTAAACCGG ACGAAGGGAG TTACGTTTGT GTTGCAAGGA ACTATCTTGG TGAAGCAGTG 
AGTCGAAATG CATCTCTGGA AGTGGCATTA TTGCGAGATG ACTTCCGGCA AAACCCCACA 
GATGTGGTAG TCGCAGCTGG AGAGCCTGCA ATCTTGGAGT GCCAGCCACC ACGGGGACAC 
CCAGAACCAA CCATCTACTG GAAAAAGGAC AAAGTCCGAA TTGATGACAA GGAAGAGAGA 
ATAAGTATCC GTGGTGGGAA GCTGATGATC TCTAATACTA GGAAAAGCGA TGCTGGCATG 
TACACCTGTG TGGGAACCAA TATGGTGGGA GAAAGGGACA GCGACCCTGC AGAGCTCACT 
GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTGCT AGAGGAAGAA 
GCTGTAGAAT TCCGTTGTCA GGTCCAAGGA GATCCCCAGC CAACGGTGAG GTGGAAAAAA 
GATGATGCAG ACTTGCCGAG AGGAAGGTAT GATATCAAAG ATGACTACAC GCTGAGAATT 
AAAAAGGCCA TGAGTACTGA TGAAGGTACC TATGTGTGTA TTGCTGAGAA TCGGGTGGGA 
AAAGTGGAAG CCTCTGCTAC CCTCACTGTC CGAGTTCGCC CTGTTGCTCC TCCACAGTTT 
GTGGTTAGGC CAAGAGATCA GATCGTTGCT CAAGGCCGAA CAGTGACATT CCCCTGTGAA 
ACTAAAGGAA ACCCACAGCC AGCTGTTTTT TGGCAGAAAG AAGGCAGCCA GAACCTACTT 
TTCCCGAATC AACCTCAGCA GCCCAACAGC CGATGTTCAG TGTCGCCCAC GGGGGACCTC 
ACCATCACCA ACATCCAGCG TTCAGATGCG GGTTACTACA TCTGCCAGGC CCTAACCGTG 
GCAGGAAGCA TTTTAGCTAA AGCACAGTTG GAAGTTACTG ACGTTTTGAC AGATAGACCT 
CCACCCATAA TCTTGCAAGG ACCAATAAAC CAAACACTTG CAGTAGACGG TACAGCATTG 
TTGAAGTGTA AAGCCACTGG TGAGCCTCTG CCTGTAATTA GCTGGCTAAA GGAGGGCTTT 
ACTTTTCTGG GGAGAGATCC AAGAGCCACG ATCCAAGACC AAGGAACACT GCAGATTAAG 
AATTTACGGA TATCTGATAC TGGCACTTAT ACTTGTGTGG CTACAAGTTC CAGTGGAGAG 
ACTTCCTGGA GTGCAGTGCT GGATGTAACA GAATCTGGAG CAACAATCAG TAAAAATTAT 
GATATGAATG ACCTCCCGGG ACCACCATCC AAACCTCAGG TCACTGATGT TTCTAAGAAC 
AGTGTCACCT TATCCTGGCA GCCAGGTACA CCTGGCGTTC TTCCTGCAAG CGCGTATATC 
ATTGAGGCTT TCAGCCAATC GGTGAGCAAT AGCTGGCAGA CAGTGGCAAA CCATGTTAAG 
ACAACTCTGT ATACAGTAAG GGGGCTGAGG CCCAACACAA TCTACTTGTT TATGGTCAGA 
GCGATCAACC CACAAGGTCT CAGTGATCCA AGTCCTATGT CGGATCCTGT ACGCACACAA 
GATATCAGCC CCCCAGCACA AGGAGTGGAC CACAGACAGG TGCAGAAGGA ATTAGGTGAT 
GTCGTTGTTC GTCTCCATAA TCCAGTTGTC CTGACACCTA CAACTGTTCA AGTCACATGG 
ACGGTGGACC GACAACCCCA GTTTATTCAG GGCTACAGAG TGATGTACCG TCAGACTTCG 
GGACTACAAG CCTCAACTGT GTGGCAGAAT CTAGACGCCA AAGTCCCGAC TGAGAGGAGT 
GCTGTCCTTG TGAATTTGAA AAAGGGGGTG ACTTATGAAA TTAAAGTCCG GCCGTATTTT 
AACGAGTTCC AAGGAATGGA CAGTGAATCG AAAACAGTCC GAACCACTGA GGAAGCCCCA 
AGTGCCCCTC CCCAGTCTGT CACTGTGCTG ACAGTTGGAA GTCACAACAG CACAAGCATC 
AGTGTTTCCT GGGATCCTCC ACCAGCCGAC CACCAGAATG GAATTATTCA GGAATATAAG 
ATCTGGTGTC TGGGAAACGA AACGCGATTC CATATCAATA AAACGGTGGA TGCAGCCATT 
CGCTCTGTAG TAATAGGTGG CTTGTTCCCT GGAATTCAGT ACCGGGTAGA AGTGGCAGCT 
AGCACAAGTG CAGGGGTTGG AGTAAAAAGT GAACCACAGC CGATAATAAT TGGGGGACGT 
AACGAAGTTG TCATTACTGA AAACAATAAC AGCATCACTG AGCAAATCAC GGATGTCGTG 
AAGCAACCGG CATTTATAGC TGGCATTGGT GGTGCCTGCT GGGTAATTCT GATGGGTTTT 
AGCATCTGGT TGTACTGGAG AAGAAAGAAG AGAAAGGGAC TCAGCAATTA TGCTGTAACA 
TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGGAGCC GTCCAGGTCT TCTAAATGCT 
GGCGATCCCA ATTACCCATG GCTTGCTGAT TCTTGGCCAG CCACGAGTTT GCCAGTGAAC 
AATAGCAATA GTGGCCCAAA TGAAATTGGA AATTTTGGGC GTGGAGATGT GCTGCCTCCG 
GTGCCAGGCC AAGGGGATAA AACAGCGACC ATGCTCTCGG ATGGAGCCAT TTATAGCAGC 
ATTGACTTCA CTACCAAAAC CACTTACAAC AGTTCCAGCC AAATAACACA GGCCACCCCA 
TATGCCACTA CACAAATCCT GCATTCAAAC AGCATCCACG AACTGGCAGT TGATCTTCCT 
GATCCACAGT GGAAAAGCTC AGTTCAACAG AAGACAGACC TCATGGGATT TGGTTATTCG 
CTACCTGATC AGAACAAGGG GAACAACGCC TTACTTTACA TCCCTGACTA CCGATTGGCT 
GAGGGATTGT CTAATAGAAT GCCACACAAC CAGTCACAGG ATTTCAGCAC CACCAGCTCT 
CACAACAGCT CAGAAAGGAG TGGCAGTCTC TCAGGTGGGA AAGGTGGAAA AAAGAAGAAA 
ACTAAAAATT CTTCGAAAGC GCAGAAAAAC AACGGATCCA CTTGGGCTAA TGTCCCTCTA 
CCTCCTCCTC CCGTCCAGCC CCTTCCTGGT ACAGAGCTGG GCCACTATGC TGCGGAACAA 
GAAAATGGCT ATGACAGTGA CAGCTGGTGC CCACCATTAC CGGTGCAAAC ATACTTGCAT 
CAGGGTATGG AAGATGAGCT GGAAGAAGAC GAAGATCGGG TCCCAACACC TCCTGTTCGC 
GGCGTGGCCT CTTCACCAGC TATCTCTTTT GGACAGCAGT CCACTGCCAC TCTTACTCCA 
TCCCCACGGG AAGAGATGCA ACCCATGCTG CAAGCTCACT TGGATGAGTT GACAAGGGCC 
TATCAGTTTG ATATAGCAAA ACAAACATGG CACATTCAAA GCAATACCCC ACCTCCACAA 
CCCCCAGCTC CGCCATTAGG TTACGTGTCC GGAGCCTTGA TTTCTGATTT GGAGACAGAT 
GTTCCAGATG AGGATGCTGA TGATGAAGAG GAACCATTAG AAATTCCCAG GCCCCTCAGA 
GCACTAGACC AGACACCTGG ATCCAGTATG GACAATCTAG ACAGCTCTGT CACAGGTTCA 
ATGGTAAATG GATGGGGCTC TGCATCTGAT GAGGATCGTA ACTTTTCTAG TCATAGATCT 
AGTGTAGGTA GCTCCTCAGA TGGCTCCATC TTTGCCAGCG GCAGCTTTGC AAAAGCATTG 
GTGGCAGCAG CAGACAAAGC TGGCTTTAGG CTGGATGGAA CCAGTCTTAC GAGAACAGGA 
AAAGCCTTTA GCTCCTCTCA AAGGCAGCGG CCCACCAGCC CATTTTCTAC GGACAGTAAC 
ACCAGTGCTG CCCAGAATCA AAGCCAGAGG CCTCGGCCCA CGAAAAAACA TAAGGGAGGA 
CGGATGGACC CACAGCCAGT GTTACCTCAT CGGAGGGAAG GGATGCCAGA TGATCTTCCC 
CCACCACCAG ATCCCCCACC AGGTCAGGGT TTAAGGCAGC AGATAGGCCT GAGCCAACAC 
TCTGGTAACG TGGAAAACTC AACAGAGAGA AAAGGAAGCT CTCTAGAGAG ACAACAAGCA 
GCCAACTTAG AAGACACAAA GAGCTCATTG GATTGTCCAG CTAAAACTGT TCTAGAGTGG 
CAGCGTCAAA CCCAGGACTG GATAAACTCT ACAGAACGCC AAGAAGAAAC TCGGAAAGCC 
CCACACAAAC AAGGGGTTGG ATCAGAGGAG TCCCTGGTGC CCTACAGCAA GCCCAGCTTC 
CCGTCTCCTG GCGGGCACAG CTCATCAGGA ACATCTTCCT CTAAAGGGTC CACTGGCCCT 
CGGAAGGCTG ACGTGCTGAG AGGCAGCCAC CAGAGGAATG CCAATGACCT TCTCGACATT 
GGATATGTGG GCTCAAATAG TCAAGGACAG TTTACAGGTG AATTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo2 ( XM_006523040.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523103.1 Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X8, mRNA.

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CloneID	OMu52760
Clone ID Related Accession (Same CDS sequence)	XM_006523040.4 , XM_006523040.3
Accession Version	XM_006523040.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4671bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	roundabout homolog 2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006523040.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT 
CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG 
GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC 
CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA 
GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT 
ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG 
GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG 
CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT 
AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT 
TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC 
GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC 
CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT 
ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA 
CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA 
TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA 
GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT 
CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT 
GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG 
CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG 
GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC 
ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG 
GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT 
GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA 
GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT 
GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG 
GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT 
GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT 
CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG 
CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG 
CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT 
TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG 
GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT 
GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT 
GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGGTG GGAAAGGTGG AAAAAAGAAG 
AAAACTAAAA ATTCTTCGAA AGCGCAGAAA AACAACGGAT CCACTTGGGC TAATGTCCCT 
CTACCTCCTC CTCCCGTCCA GCCCCTTCCT GGTACAGAGC TGGGCCACTA TGCTGCGGAA 
CAAGAAAATG GCTATGACAG TGACAGCTGG TGCCCACCAT TACCGGTGCA AACATACTTG 
CATCAGGGTA TGGAAGATGA GCTGGAAGAA GACGAAGATC GGGTCCCAAC ACCTCCTGTT 
CGCGGCGTGG CCTCTTCACC AGCTATCTCT TTTGGACAGC AGTCCACTGC CACTCTTACT 
CCATCCCCAC GGGAAGAGAT GCAACCCATG CTGCAAGCTC ACTTGGATGA GTTGACAAGG 
GCCTATCAGT TTGATATAGC AAAACAAACA TGGCACATTC AAAGCAATAC CCCACCTCCA 
CAACCCCCAG CTCCGCCATT AGGTTACGTG TCCGGAGCCT TGATTTCTGA TTTGGAGACA 
GATGTTCCAG ATGAGGATGC TGATGATGAA GAGGAACCAT TAGAAATTCC CAGGCCCCTC 
AGAGCACTAG ACCAGACACC TGGATCCAGT ATGGACAATC TAGACAGCTC TGTCACAGGT 
TCAATGGTAA ATGGATGGGG CTCTGCATCT GATGAGGATC GTAACTTTTC TAGTCATAGA 
TCTAGTGTAG GTAGCTCCTC AGATGGCTCC ATCTTTGCCA GCGGCAGCTT TGCAAAAGCA 
TTGGTGGCAG CAGCAGACAA AGCTGGCTTT AGGCTGGATG GAACCAGTCT TACGAGAACA 
GGAAAAGCCT TTAGCTCCTC TCAAAGGCAG CGGCCCACCA GCCCATTTTC TACGGACAGT 
AACACCAGTG CTGCCCAGAA TCAAAGCCAG AGGCCTCGGC CCACGAAAAA ACATAAGGGA 
GGACGGATGG ACCCACAGCC AGTGTTACCT CATCGGAGGG AAGGGATGCC AGATGATCTT 
CCCCCACCAC CAGATCCCCC ACCAGGTCAG GGTTTAAGGC AGCAGATAGG CCTGAGCCAA 
CACTCTGGTA ACGTGGAAAA CTCAACAGAG AGAAAAGGAA GCTCTCTAGA GAGACAACAA 
GCAGCCAACT TAGAAGACAC AAAGAGCTCA TTGGATTGTC CAGCTAAAAC TGTTCTAGAG 
TGGCAGCGTC AAACCCAGGA CTGGATAAAC TCTACAGAAC GCCAAGAAGA AACTCGGAAA 
GCCCCACACA AACAAGGGGT TGGATCAGAG GAGTCCCTGG TGCCCTACAG CAAGCCCAGC 
TTCCCGTCTC CTGGCGGGCA CAGCTCATCA GGAACATCTT CCTCTAAAGG GTCCACTGGC 
CCTCGGAAGG CTGACGTGCT GAGAGGCAGC CACCAGAGGA ATGCCAATGA CCTTCTCGAC 
ATTGGATATG TGGGCTCAAA TAGTCAAGGA CAGTTTACAG GTGAATTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523103.1
CDS	9440..14110
Translation	MLSSEKVDKITRLELLFVLMLFFSGPTLGKLTRTPWKGKPWKGGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKAMSTDEGTYVCIAENRVGKVEASATLTVRVRPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPINQTLAVDGTALLKCKATGEPLPVISWLKEGFTFLGRDPRATIQDQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDMNDLPGPPSKPQVTDVSKNSVTLSWQPGTPGVLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVVVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQASTVWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSHNSTSISVSWDPPPADHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVVIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGGRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPNYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTTYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSVQQKTDLMGFGYSLPDQNKGNNGGKGGKKKKTKNSSKAQKNNGSTWANVPLPPPPVQPLPGTELGHYAAEQENGYDSDSWCPPLPVQTYLHQGMEDELEEDEDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNTPPPQPPAPPLGYVSGALISDLETDVPDEDADDEEEPLEIPRPLRALDQTPGSSMDNLDSSVTGSMVNGWGSASDEDRNFSSHRSSVGSSSDGSIFASGSFAKALVAAADKAGFRLDGTSLTRTGKAFSSSQRQRPTSPFSTDSNTSAAQNQSQRPRPTKKHKGGRMDPQPVLPHRREGMPDDLPPPPDPPPGQGLRQQIGLSQHSGNVENSTERKGSSLERQQAANLEDTKSSLDCPAKTVLEWQRQTQDWINSTERQEETRKAPHKQGVGSEESLVPYSKPSFPSPGGHSSSGTSSSKGSTGPRKADVLRGSHQRNANDLLDIGYVGSNSQGQFTGEL

Target ORF information:

RefSeq Version	XM_006523040.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523040.4

ORF Insert Sequence:

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT 
CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG 
GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC 
CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA 
GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT 
ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG 
GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG 
CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT 
AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT 
TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC 
GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC 
CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT 
ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA 
CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA 
TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA 
GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT 
CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT 
GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG 
CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG 
GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC 
ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG 
GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT 
GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA 
GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT 
GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG 
GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT 
GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT 
CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG 
CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG 
CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT 
TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG 
GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT 
GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT 
GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGGTG GGAAAGGTGG AAAAAAGAAG 
AAAACTAAAA ATTCTTCGAA AGCGCAGAAA AACAACGGAT CCACTTGGGC TAATGTCCCT 
CTACCTCCTC CTCCCGTCCA GCCCCTTCCT GGTACAGAGC TGGGCCACTA TGCTGCGGAA 
CAAGAAAATG GCTATGACAG TGACAGCTGG TGCCCACCAT TACCGGTGCA AACATACTTG 
CATCAGGGTA TGGAAGATGA GCTGGAAGAA GACGAAGATC GGGTCCCAAC ACCTCCTGTT 
CGCGGCGTGG CCTCTTCACC AGCTATCTCT TTTGGACAGC AGTCCACTGC CACTCTTACT 
CCATCCCCAC GGGAAGAGAT GCAACCCATG CTGCAAGCTC ACTTGGATGA GTTGACAAGG 
GCCTATCAGT TTGATATAGC AAAACAAACA TGGCACATTC AAAGCAATAC CCCACCTCCA 
CAACCCCCAG CTCCGCCATT AGGTTACGTG TCCGGAGCCT TGATTTCTGA TTTGGAGACA 
GATGTTCCAG ATGAGGATGC TGATGATGAA GAGGAACCAT TAGAAATTCC CAGGCCCCTC 
AGAGCACTAG ACCAGACACC TGGATCCAGT ATGGACAATC TAGACAGCTC TGTCACAGGT 
TCAATGGTAA ATGGATGGGG CTCTGCATCT GATGAGGATC GTAACTTTTC TAGTCATAGA 
TCTAGTGTAG GTAGCTCCTC AGATGGCTCC ATCTTTGCCA GCGGCAGCTT TGCAAAAGCA 
TTGGTGGCAG CAGCAGACAA AGCTGGCTTT AGGCTGGATG GAACCAGTCT TACGAGAACA 
GGAAAAGCCT TTAGCTCCTC TCAAAGGCAG CGGCCCACCA GCCCATTTTC TACGGACAGT 
AACACCAGTG CTGCCCAGAA TCAAAGCCAG AGGCCTCGGC CCACGAAAAA ACATAAGGGA 
GGACGGATGG ACCCACAGCC AGTGTTACCT CATCGGAGGG AAGGGATGCC AGATGATCTT 
CCCCCACCAC CAGATCCCCC ACCAGGTCAG GGTTTAAGGC AGCAGATAGG CCTGAGCCAA 
CACTCTGGTA ACGTGGAAAA CTCAACAGAG AGAAAAGGAA GCTCTCTAGA GAGACAACAA 
GCAGCCAACT TAGAAGACAC AAAGAGCTCA TTGGATTGTC CAGCTAAAAC TGTTCTAGAG 
TGGCAGCGTC AAACCCAGGA CTGGATAAAC TCTACAGAAC GCCAAGAAGA AACTCGGAAA 
GCCCCACACA AACAAGGGGT TGGATCAGAG GAGTCCCTGG TGCCCTACAG CAAGCCCAGC 
TTCCCGTCTC CTGGCGGGCA CAGCTCATCA GGAACATCTT CCTCTAAAGG GTCCACTGGC 
CCTCGGAAGG CTGACGTGCT GAGAGGCAGC CACCAGAGGA ATGCCAATGA CCTTCTCGAC 
ATTGGATATG TGGGCTCAAA TAGTCAAGGA CAGTTTACAG GTGAATTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo2 ( XM_006523041.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523104.1 Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OMu52761
Clone ID Related Accession (Same CDS sequence)	XM_006523041.3 , XM_006523041.4
Accession Version	XM_006523041.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4614bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	roundabout homolog 2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006523041.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT 
CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG 
GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC 
CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA 
GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT 
ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG 
GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG 
CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT 
AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT 
TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC 
GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC 
CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT 
ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA 
CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA 
TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA 
GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT 
CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT 
GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG 
CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG 
GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC 
ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG 
GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT 
GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA 
GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT 
GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG 
GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT 
GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT 
CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG 
CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG 
CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT 
TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG 
GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT 
GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT 
GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGCCT TACTTTACAT CCCTGACTAC 
CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC 
ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA 
AAGAAGAAAA CTAAAAATTC TTCGAAAGCG CAGAAAAACA ACGGATCCAC TTGGGCTAAT 
GTCCCTCTAC CTCCTCCTCC CGTCCAGCCC CTTCCTGGTA CAGAGCTGGG CCACTATGCT 
GCGGAACAAG AAAATGGCTA TGACAGTGAC AGCTGGTGCC CACCATTACC GGTGCAAACA 
TACTTGCATC AGGGTATGGA AGATGAGCTG GAAGAAGACG AAGATCGGGT CCCAACACCT 
CCTGTTCGCG GCGTGGCCTC TTCACCAGCT ATCTCTTTTG GACAGCAGTC CACTGCCACT 
CTTACTCCAT CCCCACGGGA AGAGATGCAA CCCATGCTGC AAGCTCACTT GGATGAGTTG 
ACAAGGGCCT ATCAGTTTGA TATAGCAAAA CAAACATGGC ACATTCAAAG CAATACCCCA 
CCTCCACAAC CCCCAGCTCC GCCATTAGGT TACGTGTCCG GAGCCTTGAT TTCTGATTTG 
GAGACAGATG TTCCAGATGA GGATGCTGAT GATGAAGAGG AACCATTAGA AATTCCCAGG 
CCCCTCAGAG CACTAGACCA GACACCTGGA TCCAGTATGG ACAATCTAGA CAGCTCTGTC 
ACAGGAAAAG CCTTTAGCTC CTCTCAAAGG CAGCGGCCCA CCAGCCCATT TTCTACGGAC 
AGTAACACCA GTGCTGCCCA GAATCAAAGC CAGAGGCCTC GGCCCACGAA AAAACATAAG 
GGAGGACGGA TGGACCCACA GCCAGTGTTA CCTCATCGGA GGGAAGGGAT GCCAGATGAT 
CTTCCCCCAC CACCAGATCC CCCACCAGGT CAGGGTTTAA GGCAGCAGAT AGGCCTGAGC 
CAACACTCTG GTAACGTGGA AAACTCAACA GAGAGAAAAG GAAGCTCTCT AGAGAGACAA 
CAAGCAGCCA ACTTAGAAGA CACAAAGAGC TCATTGGATT GTCCAGCTAA AACTGTTCTA 
GAGTGGCAGC GTCAAACCCA GGACTGGATA AACTCTACAG AACGCCAAGA AGAAACTCGG 
AAAGCCCCAC ACAAACAAGG GGTTGGATCA GAGGAGTCCC TGGTGCCCTA CAGCAAGCCC 
AGCTTCCCGT CTCCTGGCGG GCACAGCTCA TCAGGAACAT CTTCCTCTAA AGGGTCCACT 
GGCCCTCGGA AGGCTGACGT GCTGAGAGGC AGCCACCAGA GGAATGCCAA TGACCTTCTC 
GACATTGGAT ATGTGGGCTC AAATAGTCAA GGACAGTTTA CAGGTGAATT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523104.1
CDS	9440..14053
Translation	MLSSEKVDKITRLELLFVLMLFFSGPTLGKLTRTPWKGKPWKGGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKAMSTDEGTYVCIAENRVGKVEASATLTVRVRPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPINQTLAVDGTALLKCKATGEPLPVISWLKEGFTFLGRDPRATIQDQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDMNDLPGPPSKPQVTDVSKNSVTLSWQPGTPGVLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVVVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQASTVWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSHNSTSISVSWDPPPADHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVVIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGGRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPNYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTTYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSVQQKTDLMGFGYSLPDQNKGNNALLYIPDYRLAEGLSNRMPHNQSQDFSTTSSHNSSERSGSLSGGKGGKKKKTKNSSKAQKNNGSTWANVPLPPPPVQPLPGTELGHYAAEQENGYDSDSWCPPLPVQTYLHQGMEDELEEDEDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNTPPPQPPAPPLGYVSGALISDLETDVPDEDADDEEEPLEIPRPLRALDQTPGSSMDNLDSSVTGKAFSSSQRQRPTSPFSTDSNTSAAQNQSQRPRPTKKHKGGRMDPQPVLPHRREGMPDDLPPPPDPPPGQGLRQQIGLSQHSGNVENSTERKGSSLERQQAANLEDTKSSLDCPAKTVLEWQRQTQDWINSTERQEETRKAPHKQGVGSEESLVPYSKPSFPSPGGHSSSGTSSSKGSTGPRKADVLRGSHQRNANDLLDIGYVGSNSQGQFTGEL

Target ORF information:

RefSeq Version	XM_006523041.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523041.4

ORF Insert Sequence:

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT 
CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG 
GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC 
CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA 
GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT 
ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG 
GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG 
CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT 
AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT 
TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC 
GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC 
CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT 
ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA 
CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA 
TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA 
GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT 
CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT 
GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG 
CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG 
GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC 
ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG 
GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT 
GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA 
GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT 
GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG 
GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT 
GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT 
CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG 
CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG 
CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT 
TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG 
GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT 
GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT 
GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGCCT TACTTTACAT CCCTGACTAC 
CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC 
ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA 
AAGAAGAAAA CTAAAAATTC TTCGAAAGCG CAGAAAAACA ACGGATCCAC TTGGGCTAAT 
GTCCCTCTAC CTCCTCCTCC CGTCCAGCCC CTTCCTGGTA CAGAGCTGGG CCACTATGCT 
GCGGAACAAG AAAATGGCTA TGACAGTGAC AGCTGGTGCC CACCATTACC GGTGCAAACA 
TACTTGCATC AGGGTATGGA AGATGAGCTG GAAGAAGACG AAGATCGGGT CCCAACACCT 
CCTGTTCGCG GCGTGGCCTC TTCACCAGCT ATCTCTTTTG GACAGCAGTC CACTGCCACT 
CTTACTCCAT CCCCACGGGA AGAGATGCAA CCCATGCTGC AAGCTCACTT GGATGAGTTG 
ACAAGGGCCT ATCAGTTTGA TATAGCAAAA CAAACATGGC ACATTCAAAG CAATACCCCA 
CCTCCACAAC CCCCAGCTCC GCCATTAGGT TACGTGTCCG GAGCCTTGAT TTCTGATTTG 
GAGACAGATG TTCCAGATGA GGATGCTGAT GATGAAGAGG AACCATTAGA AATTCCCAGG 
CCCCTCAGAG CACTAGACCA GACACCTGGA TCCAGTATGG ACAATCTAGA CAGCTCTGTC 
ACAGGAAAAG CCTTTAGCTC CTCTCAAAGG CAGCGGCCCA CCAGCCCATT TTCTACGGAC 
AGTAACACCA GTGCTGCCCA GAATCAAAGC CAGAGGCCTC GGCCCACGAA AAAACATAAG 
GGAGGACGGA TGGACCCACA GCCAGTGTTA CCTCATCGGA GGGAAGGGAT GCCAGATGAT 
CTTCCCCCAC CACCAGATCC CCCACCAGGT CAGGGTTTAA GGCAGCAGAT AGGCCTGAGC 
CAACACTCTG GTAACGTGGA AAACTCAACA GAGAGAAAAG GAAGCTCTCT AGAGAGACAA 
CAAGCAGCCA ACTTAGAAGA CACAAAGAGC TCATTGGATT GTCCAGCTAA AACTGTTCTA 
GAGTGGCAGC GTCAAACCCA GGACTGGATA AACTCTACAG AACGCCAAGA AGAAACTCGG 
AAAGCCCCAC ACAAACAAGG GGTTGGATCA GAGGAGTCCC TGGTGCCCTA CAGCAAGCCC 
AGCTTCCCGT CTCCTGGCGG GCACAGCTCA TCAGGAACAT CTTCCTCTAA AGGGTCCACT 
GGCCCTCGGA AGGCTGACGT GCTGAGAGGC AGCCACCAGA GGAATGCCAA TGACCTTCTC 
GACATTGGAT ATGTGGGCTC AAATAGTCAA GGACAGTTTA CAGGTGAATT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo2 ( XM_006523042.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523105.1 Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OMu52762
Clone ID Related Accession (Same CDS sequence)	XM_006523042.3 , XM_006523042.4
Accession Version	XM_006523042.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	roundabout homolog 2 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006523042.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGCTCCTCC ACAGTTTGTG 
GTTAGGCCAA GAGATCAGAT CGTTGCTCAA GGCCGAACAG TGACATTCCC CTGTGAAACT 
AAAGGAAACC CACAGCCAGC TGTTTTTTGG CAGAAAGAAG GCAGCCAGAA CCTACTTTTC 
CCGAATCAAC CTCAGCAGCC CAACAGCCGA TGTTCAGTGT CGCCCACGGG GGACCTCACC 
ATCACCAACA TCCAGCGTTC AGATGCGGGT TACTACATCT GCCAGGCCCT AACCGTGGCA 
GGAAGCATTT TAGCTAAAGC ACAGTTGGAA GTTACTGACG TTTTGACAGA TAGACCTCCA 
CCCATAATCT TGCAAGGACC AATAAACCAA ACACTTGCAG TAGACGGTAC AGCATTGTTG 
AAGTGTAAAG CCACTGGTGA GCCTCTGCCT GTAATTAGCT GGCTAAAGGA GGGCTTTACT 
TTTCTGGGGA GAGATCCAAG AGCCACGATC CAAGACCAAG GAACACTGCA GATTAAGAAT 
TTACGGATAT CTGATACTGG CACTTATACT TGTGTGGCTA CAAGTTCCAG TGGAGAGACT 
TCCTGGAGTG CAGTGCTGGA TGTAACAGAA TCTGGAGCAA CAATCAGTAA AAATTATGAT 
ATGAATGACC TCCCGGGACC ACCATCCAAA CCTCAGGTCA CTGATGTTTC TAAGAACAGT 
GTCACCTTAT CCTGGCAGCC AGGTACACCT GGCGTTCTTC CTGCAAGCGC GTATATCATT 
GAGGCTTTCA GCCAATCGGT GAGCAATAGC TGGCAGACAG TGGCAAACCA TGTTAAGACA 
ACTCTGTATA CAGTAAGGGG GCTGAGGCCC AACACAATCT ACTTGTTTAT GGTCAGAGCG 
ATCAACCCAC AAGGTCTCAG TGATCCAAGT CCTATGTCGG ATCCTGTACG CACACAAGAT 
ATCAGCCCCC CAGCACAAGG AGTGGACCAC AGACAGGTGC AGAAGGAATT AGGTGATGTC 
GTTGTTCGTC TCCATAATCC AGTTGTCCTG ACACCTACAA CTGTTCAAGT CACATGGACG 
GTGGACCGAC AACCCCAGTT TATTCAGGGC TACAGAGTGA TGTACCGTCA GACTTCGGGA 
CTACAAGCCT CAACTGTGTG GCAGAATCTA GACGCCAAAG TCCCGACTGA GAGGAGTGCT 
GTCCTTGTGA ATTTGAAAAA GGGGGTGACT TATGAAATTA AAGTCCGGCC GTATTTTAAC 
GAGTTCCAAG GAATGGACAG TGAATCGAAA ACAGTCCGAA CCACTGAGGA AGCCCCAAGT 
GCCCCTCCCC AGTCTGTCAC TGTGCTGACA GTTGGAAGTC ACAACAGCAC AAGCATCAGT 
GTTTCCTGGG ATCCTCCACC AGCCGACCAC CAGAATGGAA TTATTCAGGA ATATAAGATC 
TGGTGTCTGG GAAACGAAAC GCGATTCCAT ATCAATAAAA CGGTGGATGC AGCCATTCGC 
TCTGTAGTAA TAGGTGGCTT GTTCCCTGGA ATTCAGTACC GGGTAGAAGT GGCAGCTAGC 
ACAAGTGCAG GGGTTGGAGT AAAAAGTGAA CCACAGCCGA TAATAATTGG GGGACGTAAC 
GAAGTTGTCA TTACTGAAAA CAATAACAGC ATCACTGAGC AAATCACGGA TGTCGTGAAG 
CAACCGGCAT TTATAGCTGG CATTGGTGGT GCCTGCTGGG TAATTCTGAT GGGTTTTAGC 
ATCTGGTTGT ACTGGAGAAG AAAGAAGAGA AAGGGACTCA GCAATTATGC TGTAACATTT 
CAAAGAGGAG ATGGAGGACT AATGAGCAAT GGGAGCCGTC CAGGTCTTCT AAATGCTGGC 
GATCCCAATT ACCCATGGCT TGCTGATTCT TGGCCAGCCA CGAGTTTGCC AGTGAACAAT 
AGCAATAGTG GCCCAAATGA AATTGGAAAT TTTGGGCGTG GAGATGTGCT GCCTCCGGTG 
CCAGGCCAAG GGGATAAAAC AGCGACCATG CTCTCGGATG GAGCCATTTA TAGCAGCATT 
GACTTCACTA CCAAAACCAC TTACAACAGT TCCAGCCAAA TAACACAGGC CACCCCATAT 
GCCACTACAC AAATCCTGCA TTCAAACAGC ATCCACGAAC TGGCAGTTGA TCTTCCTGAT 
CCACAGTGGA AAAGCTCAGT TCAACAGAAG ACAGACCTCA TGGGATTTGG TTATTCGCTA 
CCTGATCAGA ACAAGGGGAA CAACGCCTTA CTTTACATCC CTGACTACCG ATTGGCTGAG 
GGATTGTCTA ATAGAATGCC ACACAACCAG TCACAGGATT TCAGCACCAC CAGCTCTCAC 
AACAGCTCAG AAAGGAGTGG CAGTCTCTCA GGTGGGAAAG GTGGAAAAAA GAAGAAAACT 
AAAAATTCTT CGAAAGCGCA GAAAAACAAC GGATCCACTT GGGCTAATGT CCCTCTACCT 
CCTCCTCCCG TCCAGCCCCT TCCTGGTACA GAGCTGGGCC ACTATGCTGC GGAACAAGAA 
AATGGCTATG ACAGTGACAG CTGGTGCCCA CCATTACCGG TGCAAACATA CTTGCATCAG 
GGTATGGAAG ATGAGCTGGA AGAAGACGAA GATCGGGTCC CAACACCTCC TGTTCGCGGC 
GTGGCCTCTT CACCAGCTAT CTCTTTTGGA CAGCAGTCCA CTGCCACTCT TACTCCATCC 
CCACGGGAAG AGATGCAACC CATGCTGCAA GCTCACTTGG ATGAGTTGAC AAGGGCCTAT 
CAGTTTGATA TAGCAAAACA AACATGGCAC ATTCAAAGCA ATACCCCACC TCCACAACCC 
CCAGCTCCGC CATTAGGTTA CGTGTCCGGA GCCTTGATTT CTGATTTGGA GACAGATGTT 
CCAGATGAGG ATGCTGATGA TGAAGAGGAA CCATTAGAAA TTCCCAGGCC CCTCAGAGCA 
CTAGACCAGA CACCTGGATC CAGTATGGAC AATCTAGACA GCTCTGTCAC AGGAAAAGCC 
TTTAGCTCCT CTCAAAGGCA GCGGCCCACC AGCCCATTTT CTACGGACAG TAACACCAGT 
GCTGCCCAGA ATCAAAGCCA GAGGCCTCGG CCCACGAAAA AACATAAGGG AGGACGGATG 
GACCCACAGC CAGTGTTACC TCATCGGAGG GAAGGGATGC CAGATGATCT TCCCCCACCA 
CCAGATCCCC CACCAGGTCA GGGTTTAAGG CAGCAGATAG GCCTGAGCCA ACACTCTGGT 
AACGTGGAAA ACTCAACAGA GAGAAAAGGA AGCTCTCTAG AGAGACAACA AGCAGCCAAC 
TTAGAAGACA CAAAGAGCTC ATTGGATTGT CCAGCTAAAA CTGTTCTAGA GTGGCAGCGT 
CAAACCCAGG ACTGGATAAA CTCTACAGAA CGCCAAGAAG AAACTCGGAA AGCCCCACAC 
AAACAAGGGG TTGGATCAGA GGAGTCCCTG GTGCCCTACA GCAAGCCCAG CTTCCCGTCT 
CCTGGCGGGC ACAGCTCATC AGGAACATCT TCCTCTAAAG GGTCCACTGG CCCTCGGAAG 
GCTGACGTGC TGAGAGGCAG CCACCAGAGG AATGCCAATG ACCTTCTCGA CATTGGATAT 
GTGGGCTCAA ATAGTCAAGG ACAGTTTACA GGTGAATTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523105.1
CDS	9440..14041
Translation	MLSSEKVDKITRLELLFVLMLFFSGPTLGKLTRTPWKGKPWKGGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKAMSTDEGTYVCIAENRVGKVEASATLTVRAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPINQTLAVDGTALLKCKATGEPLPVISWLKEGFTFLGRDPRATIQDQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDMNDLPGPPSKPQVTDVSKNSVTLSWQPGTPGVLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVVVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQASTVWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSHNSTSISVSWDPPPADHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVVIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGGRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPNYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTTYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSVQQKTDLMGFGYSLPDQNKGNNALLYIPDYRLAEGLSNRMPHNQSQDFSTTSSHNSSERSGSLSGGKGGKKKKTKNSSKAQKNNGSTWANVPLPPPPVQPLPGTELGHYAAEQENGYDSDSWCPPLPVQTYLHQGMEDELEEDEDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNTPPPQPPAPPLGYVSGALISDLETDVPDEDADDEEEPLEIPRPLRALDQTPGSSMDNLDSSVTGKAFSSSQRQRPTSPFSTDSNTSAAQNQSQRPRPTKKHKGGRMDPQPVLPHRREGMPDDLPPPPDPPPGQGLRQQIGLSQHSGNVENSTERKGSSLERQQAANLEDTKSSLDCPAKTVLEWQRQTQDWINSTERQEETRKAPHKQGVGSEESLVPYSKPSFPSPGGHSSSGTSSSKGSTGPRKADVLRGSHQRNANDLLDIGYVGSNSQGQFTGEL

Target ORF information:

RefSeq Version	XM_006523042.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523042.4

ORF Insert Sequence:

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGCTCCTCC ACAGTTTGTG 
GTTAGGCCAA GAGATCAGAT CGTTGCTCAA GGCCGAACAG TGACATTCCC CTGTGAAACT 
AAAGGAAACC CACAGCCAGC TGTTTTTTGG CAGAAAGAAG GCAGCCAGAA CCTACTTTTC 
CCGAATCAAC CTCAGCAGCC CAACAGCCGA TGTTCAGTGT CGCCCACGGG GGACCTCACC 
ATCACCAACA TCCAGCGTTC AGATGCGGGT TACTACATCT GCCAGGCCCT AACCGTGGCA 
GGAAGCATTT TAGCTAAAGC ACAGTTGGAA GTTACTGACG TTTTGACAGA TAGACCTCCA 
CCCATAATCT TGCAAGGACC AATAAACCAA ACACTTGCAG TAGACGGTAC AGCATTGTTG 
AAGTGTAAAG CCACTGGTGA GCCTCTGCCT GTAATTAGCT GGCTAAAGGA GGGCTTTACT 
TTTCTGGGGA GAGATCCAAG AGCCACGATC CAAGACCAAG GAACACTGCA GATTAAGAAT 
TTACGGATAT CTGATACTGG CACTTATACT TGTGTGGCTA CAAGTTCCAG TGGAGAGACT 
TCCTGGAGTG CAGTGCTGGA TGTAACAGAA TCTGGAGCAA CAATCAGTAA AAATTATGAT 
ATGAATGACC TCCCGGGACC ACCATCCAAA CCTCAGGTCA CTGATGTTTC TAAGAACAGT 
GTCACCTTAT CCTGGCAGCC AGGTACACCT GGCGTTCTTC CTGCAAGCGC GTATATCATT 
GAGGCTTTCA GCCAATCGGT GAGCAATAGC TGGCAGACAG TGGCAAACCA TGTTAAGACA 
ACTCTGTATA CAGTAAGGGG GCTGAGGCCC AACACAATCT ACTTGTTTAT GGTCAGAGCG 
ATCAACCCAC AAGGTCTCAG TGATCCAAGT CCTATGTCGG ATCCTGTACG CACACAAGAT 
ATCAGCCCCC CAGCACAAGG AGTGGACCAC AGACAGGTGC AGAAGGAATT AGGTGATGTC 
GTTGTTCGTC TCCATAATCC AGTTGTCCTG ACACCTACAA CTGTTCAAGT CACATGGACG 
GTGGACCGAC AACCCCAGTT TATTCAGGGC TACAGAGTGA TGTACCGTCA GACTTCGGGA 
CTACAAGCCT CAACTGTGTG GCAGAATCTA GACGCCAAAG TCCCGACTGA GAGGAGTGCT 
GTCCTTGTGA ATTTGAAAAA GGGGGTGACT TATGAAATTA AAGTCCGGCC GTATTTTAAC 
GAGTTCCAAG GAATGGACAG TGAATCGAAA ACAGTCCGAA CCACTGAGGA AGCCCCAAGT 
GCCCCTCCCC AGTCTGTCAC TGTGCTGACA GTTGGAAGTC ACAACAGCAC AAGCATCAGT 
GTTTCCTGGG ATCCTCCACC AGCCGACCAC CAGAATGGAA TTATTCAGGA ATATAAGATC 
TGGTGTCTGG GAAACGAAAC GCGATTCCAT ATCAATAAAA CGGTGGATGC AGCCATTCGC 
TCTGTAGTAA TAGGTGGCTT GTTCCCTGGA ATTCAGTACC GGGTAGAAGT GGCAGCTAGC 
ACAAGTGCAG GGGTTGGAGT AAAAAGTGAA CCACAGCCGA TAATAATTGG GGGACGTAAC 
GAAGTTGTCA TTACTGAAAA CAATAACAGC ATCACTGAGC AAATCACGGA TGTCGTGAAG 
CAACCGGCAT TTATAGCTGG CATTGGTGGT GCCTGCTGGG TAATTCTGAT GGGTTTTAGC 
ATCTGGTTGT ACTGGAGAAG AAAGAAGAGA AAGGGACTCA GCAATTATGC TGTAACATTT 
CAAAGAGGAG ATGGAGGACT AATGAGCAAT GGGAGCCGTC CAGGTCTTCT AAATGCTGGC 
GATCCCAATT ACCCATGGCT TGCTGATTCT TGGCCAGCCA CGAGTTTGCC AGTGAACAAT 
AGCAATAGTG GCCCAAATGA AATTGGAAAT TTTGGGCGTG GAGATGTGCT GCCTCCGGTG 
CCAGGCCAAG GGGATAAAAC AGCGACCATG CTCTCGGATG GAGCCATTTA TAGCAGCATT 
GACTTCACTA CCAAAACCAC TTACAACAGT TCCAGCCAAA TAACACAGGC CACCCCATAT 
GCCACTACAC AAATCCTGCA TTCAAACAGC ATCCACGAAC TGGCAGTTGA TCTTCCTGAT 
CCACAGTGGA AAAGCTCAGT TCAACAGAAG ACAGACCTCA TGGGATTTGG TTATTCGCTA 
CCTGATCAGA ACAAGGGGAA CAACGCCTTA CTTTACATCC CTGACTACCG ATTGGCTGAG 
GGATTGTCTA ATAGAATGCC ACACAACCAG TCACAGGATT TCAGCACCAC CAGCTCTCAC 
AACAGCTCAG AAAGGAGTGG CAGTCTCTCA GGTGGGAAAG GTGGAAAAAA GAAGAAAACT 
AAAAATTCTT CGAAAGCGCA GAAAAACAAC GGATCCACTT GGGCTAATGT CCCTCTACCT 
CCTCCTCCCG TCCAGCCCCT TCCTGGTACA GAGCTGGGCC ACTATGCTGC GGAACAAGAA 
AATGGCTATG ACAGTGACAG CTGGTGCCCA CCATTACCGG TGCAAACATA CTTGCATCAG 
GGTATGGAAG ATGAGCTGGA AGAAGACGAA GATCGGGTCC CAACACCTCC TGTTCGCGGC 
GTGGCCTCTT CACCAGCTAT CTCTTTTGGA CAGCAGTCCA CTGCCACTCT TACTCCATCC 
CCACGGGAAG AGATGCAACC CATGCTGCAA GCTCACTTGG ATGAGTTGAC AAGGGCCTAT 
CAGTTTGATA TAGCAAAACA AACATGGCAC ATTCAAAGCA ATACCCCACC TCCACAACCC 
CCAGCTCCGC CATTAGGTTA CGTGTCCGGA GCCTTGATTT CTGATTTGGA GACAGATGTT 
CCAGATGAGG ATGCTGATGA TGAAGAGGAA CCATTAGAAA TTCCCAGGCC CCTCAGAGCA 
CTAGACCAGA CACCTGGATC CAGTATGGAC AATCTAGACA GCTCTGTCAC AGGAAAAGCC 
TTTAGCTCCT CTCAAAGGCA GCGGCCCACC AGCCCATTTT CTACGGACAG TAACACCAGT 
GCTGCCCAGA ATCAAAGCCA GAGGCCTCGG CCCACGAAAA AACATAAGGG AGGACGGATG 
GACCCACAGC CAGTGTTACC TCATCGGAGG GAAGGGATGC CAGATGATCT TCCCCCACCA 
CCAGATCCCC CACCAGGTCA GGGTTTAAGG CAGCAGATAG GCCTGAGCCA ACACTCTGGT 
AACGTGGAAA ACTCAACAGA GAGAAAAGGA AGCTCTCTAG AGAGACAACA AGCAGCCAAC 
TTAGAAGACA CAAAGAGCTC ATTGGATTGT CCAGCTAAAA CTGTTCTAGA GTGGCAGCGT 
CAAACCCAGG ACTGGATAAA CTCTACAGAA CGCCAAGAAG AAACTCGGAA AGCCCCACAC 
AAACAAGGGG TTGGATCAGA GGAGTCCCTG GTGCCCTACA GCAAGCCCAG CTTCCCGTCT 
CCTGGCGGGC ACAGCTCATC AGGAACATCT TCCTCTAAAG GGTCCACTGG CCCTCGGAAG 
GCTGACGTGC TGAGAGGCAG CCACCAGAGG AATGCCAATG ACCTTCTCGA CATTGGATAT 
GTGGGCTCAA ATAGTCAAGG ACAGTTTACA GGTGAATTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo2 ( XM_006523043.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523106.1 Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X11, mRNA.

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CloneID	OMu52763
Clone ID Related Accession (Same CDS sequence)	XM_006523043.3 , XM_006523043.4
Accession Version	XM_006523043.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	roundabout homolog 2 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006523043.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCCTC TGATGTTTAC ACTATTATTG CTCTTTGGAT TTCTCTGCAT TCAGATTGAT 
GGATCTCGTC TTCGTCAAGA AGACTTTCCC CCCAGGATCG TAGAACACCC TTCTGATGTC 
ATCGTCTCCA AGGGAGAGCC CACCACTCTG AACTGTAAAG CAGAGGGCCG ACCCACCCCC 
ACCATTGAAT GGTACAAGGA TGGTGAGAGG GTGGAGACAG ACAAGGATGA TCCCAGGTCC 
CACAGAATGC TTCTGCCCAG CGGATCTTTA TTCTTTTTGC GAATTGTTCA TGGGCGCAGA 
AGTAAACCGG ACGAAGGGAG TTACGTTTGT GTTGCAAGGA ACTATCTTGG TGAAGCAGTG 
AGTCGAAATG CATCTCTGGA AGTGGCATTA TTGCGAGATG ACTTCCGGCA AAACCCCACA 
GATGTGGTAG TCGCAGCTGG AGAGCCTGCA ATCTTGGAGT GCCAGCCACC ACGGGGACAC 
CCAGAACCAA CCATCTACTG GAAAAAGGAC AAAGTCCGAA TTGATGACAA GGAAGAGAGA 
ATAAGTATCC GTGGTGGGAA GCTGATGATC TCTAATACTA GGAAAAGCGA TGCTGGCATG 
TACACCTGTG TGGGAACCAA TATGGTGGGA GAAAGGGACA GCGACCCTGC AGAGCTCACT 
GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTGCT AGAGGAAGAA 
GCTGTAGAAT TCCGTTGTCA GGTCCAAGGA GATCCCCAGC CAACGGTGAG GTGGAAAAAA 
GATGATGCAG ACTTGCCGAG AGGAAGGTAT GATATCAAAG ATGACTACAC GCTGAGAATT 
AAAAAGGCCA TGAGTACTGA TGAAGGTACC TATGTGTGTA TTGCTGAGAA TCGGGTGGGA 
AAAGTGGAAG CCTCTGCTAC CCTCACTGTC CGAGTTCGCC CTGTTGCTCC TCCACAGTTT 
GTGGTTAGGC CAAGAGATCA GATCGTTGCT CAAGGCCGAA CAGTGACATT CCCCTGTGAA 
ACTAAAGGAA ACCCACAGCC AGCTGTTTTT TGGCAGAAAG AAGGCAGCCA GAACCTACTT 
TTCCCGAATC AACCTCAGCA GCCCAACAGC CGATGTTCAG TGTCGCCCAC GGGGGACCTC 
ACCATCACCA ACATCCAGCG TTCAGATGCG GGTTACTACA TCTGCCAGGC CCTAACCGTG 
GCAGGAAGCA TTTTAGCTAA AGCACAGTTG GAAGTTACTG ACGTTTTGAC AGATAGACCT 
CCACCCATAA TCTTGCAAGG ACCAATAAAC CAAACACTTG CAGTAGACGG TACAGCATTG 
TTGAAGTGTA AAGCCACTGG TGAGCCTCTG CCTGTAATTA GCTGGCTAAA GGAGGGCTTT 
ACTTTTCTGG GGAGAGATCC AAGAGCCACG ATCCAAGACC AAGGAACACT GCAGATTAAG 
AATTTACGGA TATCTGATAC TGGCACTTAT ACTTGTGTGG CTACAAGTTC CAGTGGAGAG 
ACTTCCTGGA GTGCAGTGCT GGATGTAACA GAATCTGGAG CAACAATCAG TAAAAATTAT 
GATATGAATG ACCTCCCGGG ACCACCATCC AAACCTCAGG TCACTGATGT TTCTAAGAAC 
AGTGTCACCT TATCCTGGCA GCCAGGTACA CCTGGCGTTC TTCCTGCAAG CGCGTATATC 
ATTGAGGCTT TCAGCCAATC GGTGAGCAAT AGCTGGCAGA CAGTGGCAAA CCATGTTAAG 
ACAACTCTGT ATACAGTAAG GGGGCTGAGG CCCAACACAA TCTACTTGTT TATGGTCAGA 
GCGATCAACC CACAAGGTCT CAGTGATCCA AGTCCTATGT CGGATCCTGT ACGCACACAA 
GATATCAGCC CCCCAGCACA AGGAGTGGAC CACAGACAGG TGCAGAAGGA ATTAGGTGAT 
GTCGTTGTTC GTCTCCATAA TCCAGTTGTC CTGACACCTA CAACTGTTCA AGTCACATGG 
ACGGTGGACC GACAACCCCA GTTTATTCAG GGCTACAGAG TGATGTACCG TCAGACTTCG 
GGACTACAAG CCTCAACTGT GTGGCAGAAT CTAGACGCCA AAGTCCCGAC TGAGAGGAGT 
GCTGTCCTTG TGAATTTGAA AAAGGGGGTG ACTTATGAAA TTAAAGTCCG GCCGTATTTT 
AACGAGTTCC AAGGAATGGA CAGTGAATCG AAAACAGTCC GAACCACTGA GGAAGCCCCA 
AGTGCCCCTC CCCAGTCTGT CACTGTGCTG ACAGTTGGAA GTCACAACAG CACAAGCATC 
AGTGTTTCCT GGGATCCTCC ACCAGCCGAC CACCAGAATG GAATTATTCA GGAATATAAG 
ATCTGGTGTC TGGGAAACGA AACGCGATTC CATATCAATA AAACGGTGGA TGCAGCCATT 
CGCTCTGTAG TAATAGGTGG CTTGTTCCCT GGAATTCAGT ACCGGGTAGA AGTGGCAGCT 
AGCACAAGTG CAGGGGTTGG AGTAAAAAGT GAACCACAGC CGATAATAAT TGGGGGACGT 
AACGAAGTTG TCATTACTGA AAACAATAAC AGCATCACTG AGCAAATCAC GGATGTCGTG 
AAGCAACCGG CATTTATAGC TGGCATTGGT GGTGCCTGCT GGGTAATTCT GATGGGTTTT 
AGCATCTGGT TGTACTGGAG AAGAAAGAAG AGAAAGGGAC TCAGCAATTA TGCTGTAACA 
TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGGAGCC GTCCAGGTCT TCTAAATGCT 
GGCGATCCCA ATTACCCATG GCTTGCTGAT TCTTGGCCAG CCACGAGTTT GCCAGTGAAC 
AATAGCAATA GTGGCCCAAA TGAAATTGGA AATTTTGGGC GTGGAGATGT GCTGCCTCCG 
GTGCCAGGCC AAGGGGATAA AACAGCGACC ATGCTCTCGG ATGGAGCCAT TTATAGCAGC 
ATTGACTTCA CTACCAAAAC CACTTACAAC AGTTCCAGCC AAATAACACA GGCCACCCCA 
TATGCCACTA CACAAATCCT GCATTCAAAC AGCATCCACG AACTGGCAGT TGATCTTCCT 
GATCCACAGT GGAAAAGCTC AGTTCAACAG AAGACAGACC TCATGGGATT TGGTTATTCG 
CTACCTGATC AGAACAAGGG GAACAACGGT GGGAAAGGTG GAAAAAAGAA GAAAACTAAA 
AATTCTTCGA AAGCGCAGAA AAACAACGGA TCCACTTGGG CTAATGTCCC TCTACCTCCT 
CCTCCCGTCC AGCCCCTTCC TGGTACAGAG CTGGGCCACT ATGCTGCGGA ACAAGAAAAT 
GGCTATGACA GTGACAGCTG GTGCCCACCA TTACCGGTGC AAACATACTT GCATCAGGGT 
ATGGAAGATG AGCTGGAAGA AGACGAAGAT CGGGTCCCAA CACCTCCTGT TCGCGGCGTG 
GCCTCTTCAC CAGCTATCTC TTTTGGACAG CAGTCCACTG CCACTCTTAC TCCATCCCCA 
CGGGAAGAGA TGCAACCCAT GCTGCAAGCT CACTTGGATG AGTTGACAAG GGCCTATCAG 
TTTGATATAG CAAAACAAAC ATGGCACATT CAAAGCAATA CCCCACCTCC ACAACCCCCA 
GCTCCGCCAT TAGGTTACGT GTCCGGAGCC TTGATTTCTG ATTTGGAGAC AGATGTTCCA 
GATGAGGATG CTGATGATGA AGAGGAACCA TTAGAAATTC CCAGGCCCCT CAGAGCACTA 
GACCAGACAC CTGGATCCAG TATGGACAAT CTAGACAGCT CTGTCACAGG TTCAATGGTA 
AATGGATGGG GCTCTGCATC TGATGAGGAT CGTAACTTTT CTAGTCATAG ATCTAGTGTA 
GGTAGCTCCT CAGATGGCTC CATCTTTGCC AGCGGCAGCT TTGCAAAAGC ATTGGTGGCA 
GCAGCAGACA AAGCTGGCTT TAGGCTGGAT GGAACCAGTC TTACGAGAAC AGGAAAAGCC 
TTTAGCTCCT CTCAAAGGCA GCGGCCCACC AGCCCATTTT CTACGGACAG TAACACCAGT 
GCTGCCCAGA ATCAAAGCCA GAGGCCTCGG CCCACGAAAA AACATAAGGG AGGACGGATG 
GACCCACAGC CAGTGTTACC TCATCGGAGG GAAGGGATGC CAGATGATCT TCCCCCACCA 
CCAGATCCCC CACCAGGTCA GGGTTTAAGG CAGCAGATAG GCCTGAGCCA ACACTCTGGT 
AACGTGGAAA ACTCAACAGA GAGAAAAGGA AGCTCTCTAG AGAGACAACA AGCAGCCAAC 
TTAGAAGACA CAAAGAGCTC ATTGGATTGT CCAGCTAAAA CTGTTCTAGA GTGGCAGCGT 
CAAACCCAGG ACTGGATAAA CTCTACAGAA CGCCAAGAAG AAACTCGGAA AGCCCCACAC 
AAACAAGGGG TTGGATCAGA GGAGTCCCTG GTGCCCTACA GCAAGCCCAG CTTCCCGTCT 
CCTGGCGGGC ACAGCTCATC AGGAACATCT TCCTCTAAAG GGTCCACTGG CCCTCGGAAG 
GCTGACGTGC TGAGAGGCAG CCACCAGAGG AATGCCAATG ACCTTCTCGA CATTGGATAT 
GTGGGCTCAA ATAGTCAAGG ACAGTTTACA GGTGAATTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523106.1
CDS	2416..7017
Translation	MNPLMFTLLLLFGFLCIQIDGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKAMSTDEGTYVCIAENRVGKVEASATLTVRVRPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPINQTLAVDGTALLKCKATGEPLPVISWLKEGFTFLGRDPRATIQDQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDMNDLPGPPSKPQVTDVSKNSVTLSWQPGTPGVLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVVVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQASTVWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSHNSTSISVSWDPPPADHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVVIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGGRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPNYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTTYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSVQQKTDLMGFGYSLPDQNKGNNGGKGGKKKKTKNSSKAQKNNGSTWANVPLPPPPVQPLPGTELGHYAAEQENGYDSDSWCPPLPVQTYLHQGMEDELEEDEDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNTPPPQPPAPPLGYVSGALISDLETDVPDEDADDEEEPLEIPRPLRALDQTPGSSMDNLDSSVTGSMVNGWGSASDEDRNFSSHRSSVGSSSDGSIFASGSFAKALVAAADKAGFRLDGTSLTRTGKAFSSSQRQRPTSPFSTDSNTSAAQNQSQRPRPTKKHKGGRMDPQPVLPHRREGMPDDLPPPPDPPPGQGLRQQIGLSQHSGNVENSTERKGSSLERQQAANLEDTKSSLDCPAKTVLEWQRQTQDWINSTERQEETRKAPHKQGVGSEESLVPYSKPSFPSPGGHSSSGTSSSKGSTGPRKADVLRGSHQRNANDLLDIGYVGSNSQGQFTGEL

Target ORF information:

RefSeq Version	XM_006523043.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523043.4

ORF Insert Sequence:

ATGAATCCTC TGATGTTTAC ACTATTATTG CTCTTTGGAT TTCTCTGCAT TCAGATTGAT 
GGATCTCGTC TTCGTCAAGA AGACTTTCCC CCCAGGATCG TAGAACACCC TTCTGATGTC 
ATCGTCTCCA AGGGAGAGCC CACCACTCTG AACTGTAAAG CAGAGGGCCG ACCCACCCCC 
ACCATTGAAT GGTACAAGGA TGGTGAGAGG GTGGAGACAG ACAAGGATGA TCCCAGGTCC 
CACAGAATGC TTCTGCCCAG CGGATCTTTA TTCTTTTTGC GAATTGTTCA TGGGCGCAGA 
AGTAAACCGG ACGAAGGGAG TTACGTTTGT GTTGCAAGGA ACTATCTTGG TGAAGCAGTG 
AGTCGAAATG CATCTCTGGA AGTGGCATTA TTGCGAGATG ACTTCCGGCA AAACCCCACA 
GATGTGGTAG TCGCAGCTGG AGAGCCTGCA ATCTTGGAGT GCCAGCCACC ACGGGGACAC 
CCAGAACCAA CCATCTACTG GAAAAAGGAC AAAGTCCGAA TTGATGACAA GGAAGAGAGA 
ATAAGTATCC GTGGTGGGAA GCTGATGATC TCTAATACTA GGAAAAGCGA TGCTGGCATG 
TACACCTGTG TGGGAACCAA TATGGTGGGA GAAAGGGACA GCGACCCTGC AGAGCTCACT 
GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTGCT AGAGGAAGAA 
GCTGTAGAAT TCCGTTGTCA GGTCCAAGGA GATCCCCAGC CAACGGTGAG GTGGAAAAAA 
GATGATGCAG ACTTGCCGAG AGGAAGGTAT GATATCAAAG ATGACTACAC GCTGAGAATT 
AAAAAGGCCA TGAGTACTGA TGAAGGTACC TATGTGTGTA TTGCTGAGAA TCGGGTGGGA 
AAAGTGGAAG CCTCTGCTAC CCTCACTGTC CGAGTTCGCC CTGTTGCTCC TCCACAGTTT 
GTGGTTAGGC CAAGAGATCA GATCGTTGCT CAAGGCCGAA CAGTGACATT CCCCTGTGAA 
ACTAAAGGAA ACCCACAGCC AGCTGTTTTT TGGCAGAAAG AAGGCAGCCA GAACCTACTT 
TTCCCGAATC AACCTCAGCA GCCCAACAGC CGATGTTCAG TGTCGCCCAC GGGGGACCTC 
ACCATCACCA ACATCCAGCG TTCAGATGCG GGTTACTACA TCTGCCAGGC CCTAACCGTG 
GCAGGAAGCA TTTTAGCTAA AGCACAGTTG GAAGTTACTG ACGTTTTGAC AGATAGACCT 
CCACCCATAA TCTTGCAAGG ACCAATAAAC CAAACACTTG CAGTAGACGG TACAGCATTG 
TTGAAGTGTA AAGCCACTGG TGAGCCTCTG CCTGTAATTA GCTGGCTAAA GGAGGGCTTT 
ACTTTTCTGG GGAGAGATCC AAGAGCCACG ATCCAAGACC AAGGAACACT GCAGATTAAG 
AATTTACGGA TATCTGATAC TGGCACTTAT ACTTGTGTGG CTACAAGTTC CAGTGGAGAG 
ACTTCCTGGA GTGCAGTGCT GGATGTAACA GAATCTGGAG CAACAATCAG TAAAAATTAT 
GATATGAATG ACCTCCCGGG ACCACCATCC AAACCTCAGG TCACTGATGT TTCTAAGAAC 
AGTGTCACCT TATCCTGGCA GCCAGGTACA CCTGGCGTTC TTCCTGCAAG CGCGTATATC 
ATTGAGGCTT TCAGCCAATC GGTGAGCAAT AGCTGGCAGA CAGTGGCAAA CCATGTTAAG 
ACAACTCTGT ATACAGTAAG GGGGCTGAGG CCCAACACAA TCTACTTGTT TATGGTCAGA 
GCGATCAACC CACAAGGTCT CAGTGATCCA AGTCCTATGT CGGATCCTGT ACGCACACAA 
GATATCAGCC CCCCAGCACA AGGAGTGGAC CACAGACAGG TGCAGAAGGA ATTAGGTGAT 
GTCGTTGTTC GTCTCCATAA TCCAGTTGTC CTGACACCTA CAACTGTTCA AGTCACATGG 
ACGGTGGACC GACAACCCCA GTTTATTCAG GGCTACAGAG TGATGTACCG TCAGACTTCG 
GGACTACAAG CCTCAACTGT GTGGCAGAAT CTAGACGCCA AAGTCCCGAC TGAGAGGAGT 
GCTGTCCTTG TGAATTTGAA AAAGGGGGTG ACTTATGAAA TTAAAGTCCG GCCGTATTTT 
AACGAGTTCC AAGGAATGGA CAGTGAATCG AAAACAGTCC GAACCACTGA GGAAGCCCCA 
AGTGCCCCTC CCCAGTCTGT CACTGTGCTG ACAGTTGGAA GTCACAACAG CACAAGCATC 
AGTGTTTCCT GGGATCCTCC ACCAGCCGAC CACCAGAATG GAATTATTCA GGAATATAAG 
ATCTGGTGTC TGGGAAACGA AACGCGATTC CATATCAATA AAACGGTGGA TGCAGCCATT 
CGCTCTGTAG TAATAGGTGG CTTGTTCCCT GGAATTCAGT ACCGGGTAGA AGTGGCAGCT 
AGCACAAGTG CAGGGGTTGG AGTAAAAAGT GAACCACAGC CGATAATAAT TGGGGGACGT 
AACGAAGTTG TCATTACTGA AAACAATAAC AGCATCACTG AGCAAATCAC GGATGTCGTG 
AAGCAACCGG CATTTATAGC TGGCATTGGT GGTGCCTGCT GGGTAATTCT GATGGGTTTT 
AGCATCTGGT TGTACTGGAG AAGAAAGAAG AGAAAGGGAC TCAGCAATTA TGCTGTAACA 
TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGGAGCC GTCCAGGTCT TCTAAATGCT 
GGCGATCCCA ATTACCCATG GCTTGCTGAT TCTTGGCCAG CCACGAGTTT GCCAGTGAAC 
AATAGCAATA GTGGCCCAAA TGAAATTGGA AATTTTGGGC GTGGAGATGT GCTGCCTCCG 
GTGCCAGGCC AAGGGGATAA AACAGCGACC ATGCTCTCGG ATGGAGCCAT TTATAGCAGC 
ATTGACTTCA CTACCAAAAC CACTTACAAC AGTTCCAGCC AAATAACACA GGCCACCCCA 
TATGCCACTA CACAAATCCT GCATTCAAAC AGCATCCACG AACTGGCAGT TGATCTTCCT 
GATCCACAGT GGAAAAGCTC AGTTCAACAG AAGACAGACC TCATGGGATT TGGTTATTCG 
CTACCTGATC AGAACAAGGG GAACAACGGT GGGAAAGGTG GAAAAAAGAA GAAAACTAAA 
AATTCTTCGA AAGCGCAGAA AAACAACGGA TCCACTTGGG CTAATGTCCC TCTACCTCCT 
CCTCCCGTCC AGCCCCTTCC TGGTACAGAG CTGGGCCACT ATGCTGCGGA ACAAGAAAAT 
GGCTATGACA GTGACAGCTG GTGCCCACCA TTACCGGTGC AAACATACTT GCATCAGGGT 
ATGGAAGATG AGCTGGAAGA AGACGAAGAT CGGGTCCCAA CACCTCCTGT TCGCGGCGTG 
GCCTCTTCAC CAGCTATCTC TTTTGGACAG CAGTCCACTG CCACTCTTAC TCCATCCCCA 
CGGGAAGAGA TGCAACCCAT GCTGCAAGCT CACTTGGATG AGTTGACAAG GGCCTATCAG 
TTTGATATAG CAAAACAAAC ATGGCACATT CAAAGCAATA CCCCACCTCC ACAACCCCCA 
GCTCCGCCAT TAGGTTACGT GTCCGGAGCC TTGATTTCTG ATTTGGAGAC AGATGTTCCA 
GATGAGGATG CTGATGATGA AGAGGAACCA TTAGAAATTC CCAGGCCCCT CAGAGCACTA 
GACCAGACAC CTGGATCCAG TATGGACAAT CTAGACAGCT CTGTCACAGG TTCAATGGTA 
AATGGATGGG GCTCTGCATC TGATGAGGAT CGTAACTTTT CTAGTCATAG ATCTAGTGTA 
GGTAGCTCCT CAGATGGCTC CATCTTTGCC AGCGGCAGCT TTGCAAAAGC ATTGGTGGCA 
GCAGCAGACA AAGCTGGCTT TAGGCTGGAT GGAACCAGTC TTACGAGAAC AGGAAAAGCC 
TTTAGCTCCT CTCAAAGGCA GCGGCCCACC AGCCCATTTT CTACGGACAG TAACACCAGT 
GCTGCCCAGA ATCAAAGCCA GAGGCCTCGG CCCACGAAAA AACATAAGGG AGGACGGATG 
GACCCACAGC CAGTGTTACC TCATCGGAGG GAAGGGATGC CAGATGATCT TCCCCCACCA 
CCAGATCCCC CACCAGGTCA GGGTTTAAGG CAGCAGATAG GCCTGAGCCA ACACTCTGGT 
AACGTGGAAA ACTCAACAGA GAGAAAAGGA AGCTCTCTAG AGAGACAACA AGCAGCCAAC 
TTAGAAGACA CAAAGAGCTC ATTGGATTGT CCAGCTAAAA CTGTTCTAGA GTGGCAGCGT 
CAAACCCAGG ACTGGATAAA CTCTACAGAA CGCCAAGAAG AAACTCGGAA AGCCCCACAC 
AAACAAGGGG TTGGATCAGA GGAGTCCCTG GTGCCCTACA GCAAGCCCAG CTTCCCGTCT 
CCTGGCGGGC ACAGCTCATC AGGAACATCT TCCTCTAAAG GGTCCACTGG CCCTCGGAAG 
GCTGACGTGC TGAGAGGCAG CCACCAGAGG AATGCCAATG ACCTTCTCGA CATTGGATAT 
GTGGGCTCAA ATAGTCAAGG ACAGTTTACA GGTGAATTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo2 ( XM_006523045.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523108.1 Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X12, mRNA.

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CloneID	OMu52765
Clone ID Related Accession (Same CDS sequence)	XM_006523045.4 , XM_006523045.3
Accession Version	XM_006523045.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	roundabout homolog 2 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006523045.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT 
CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG 
GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC 
CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA 
GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT 
ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG 
GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG 
CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT 
AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT 
TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC 
GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC 
CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT 
ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA 
CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA 
TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA 
GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT 
CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT 
GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG 
CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG 
GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC 
ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG 
GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT 
GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA 
GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT 
GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG 
GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT 
GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT 
CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG 
CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG 
CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT 
TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG 
GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT 
GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT 
GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGCCT TACTTTACAT CCCTGACTAC 
CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC 
ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA 
AAGAAGAAAA CTAAAAATTC TTCGAAAGCG CAGAAAAACA ACGGATCCAC TTGGGCTAAT 
GTCCCTCTAC CTCCTCCTCC CGTCCAGCCC CTTCCTGGTA CAGAGCTGGG CCACTATGCT 
GCGGAACAAG AAAATGGCTA TGACAGTGAC AGCTGGTGCC CACCATTACC GGTGCAAACA 
TACTTGCATC AGGGTATGGA AGATGAGCTG GAAGAAGACG AAGATCGGGT CCCAACACCT 
CCTGTTCGCG GCGTGGCCTC TTCACCAGCT ATCTCTTTTG GACAGCAGTC CACTGCCACT 
CTTACTCCAT CCCCACGGGA AGAGATGCAA CCCATGCTGC AAGCTCACTT GGATGAGTTG 
ACAAGGGCCT ATCAGTTTGA TATAGCAAAA CAAACATGGC ACATTCAAAG CAATACCCCA 
CCTCCACAAC CCCCAGCTCC GCCATTAGGT TACGTGTCCG GAGCCTTGAT TTCTGATTTG 
GAGACAGATG TTCCAGATGA GGATGCTGAT GATGAAGAGG AACCATTAGA AATTCCCAGG 
CCCCTCAGAG CACTAGACCA GACACCTGGA TCCAGTATGG ACAATCTAGA CAGCTCTGTC 
ACAGGTTCAA TGGTAAATGG ATGGGGCTCT GCATCTGATG AGGATCGTAA CTTTTCTAGT 
CATAGATCTA GTGTAGGTAG CTCCTCAGAT GGCTCCATCT TTGCCAGCGG CAGCTTTGCA 
AAAGCATTGG TGGCAGCAGC AGACAAAGCT GGCTTTAGGC TGGATGGAAC CAGTCTTACG 
AGAACAGGAA AAGCCTTTAG CTCCTCTCAA AGGCAGCGGC CCACCAGCCC ATTTTCTACG 
GACAGTAACA CCAGTGCTGC CCAGAATCAA AGCCAGAGGC CTCGGCCCAC GAAAAAACAT 
AAGGGAGGAC GGATGGACCC ACAGCCAGTG TTACCTCATC GGAGGGAAGG GATGCCAGAT 
GAGGAGTCCC TGGTGCCCTA CAGCAAGCCC AGCTTCCCGT CTCCTGGCGG GCACAGCTCA 
TCAGGAACAT CTTCCTCTAA AGGGTCCACT GGCCCTCGGA AGGCTGACGT GCTGAGAGGC 
AGCCACCAGA GGAATGCCAA TGACCTTCTC GACATTGGAT ATGTGGGCTC AAATAGTCAA 
GGACAGTTTA CAGGTGAATT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523108.1
CDS	9440..13963
Translation	MLSSEKVDKITRLELLFVLMLFFSGPTLGKLTRTPWKGKPWKGGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKAMSTDEGTYVCIAENRVGKVEASATLTVRVRPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPINQTLAVDGTALLKCKATGEPLPVISWLKEGFTFLGRDPRATIQDQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDMNDLPGPPSKPQVTDVSKNSVTLSWQPGTPGVLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVVVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQASTVWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSHNSTSISVSWDPPPADHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVVIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGGRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPNYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTTYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSVQQKTDLMGFGYSLPDQNKGNNALLYIPDYRLAEGLSNRMPHNQSQDFSTTSSHNSSERSGSLSGGKGGKKKKTKNSSKAQKNNGSTWANVPLPPPPVQPLPGTELGHYAAEQENGYDSDSWCPPLPVQTYLHQGMEDELEEDEDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNTPPPQPPAPPLGYVSGALISDLETDVPDEDADDEEEPLEIPRPLRALDQTPGSSMDNLDSSVTGSMVNGWGSASDEDRNFSSHRSSVGSSSDGSIFASGSFAKALVAAADKAGFRLDGTSLTRTGKAFSSSQRQRPTSPFSTDSNTSAAQNQSQRPRPTKKHKGGRMDPQPVLPHRREGMPDEESLVPYSKPSFPSPGGHSSSGTSSSKGSTGPRKADVLRGSHQRNANDLLDIGYVGSNSQGQFTGEL

Target ORF information:

RefSeq Version	XM_006523045.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523045.4

ORF Insert Sequence:

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT 
CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG 
GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC 
CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA 
GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT 
ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG 
GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG 
CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT 
AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT 
TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC 
GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC 
CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT 
ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA 
CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA 
TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA 
GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT 
CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT 
GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG 
CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG 
GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC 
ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG 
GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT 
GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA 
GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT 
GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG 
GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT 
GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT 
CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG 
CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG 
CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT 
TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG 
GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT 
GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT 
GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGCCT TACTTTACAT CCCTGACTAC 
CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC 
ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA 
AAGAAGAAAA CTAAAAATTC TTCGAAAGCG CAGAAAAACA ACGGATCCAC TTGGGCTAAT 
GTCCCTCTAC CTCCTCCTCC CGTCCAGCCC CTTCCTGGTA CAGAGCTGGG CCACTATGCT 
GCGGAACAAG AAAATGGCTA TGACAGTGAC AGCTGGTGCC CACCATTACC GGTGCAAACA 
TACTTGCATC AGGGTATGGA AGATGAGCTG GAAGAAGACG AAGATCGGGT CCCAACACCT 
CCTGTTCGCG GCGTGGCCTC TTCACCAGCT ATCTCTTTTG GACAGCAGTC CACTGCCACT 
CTTACTCCAT CCCCACGGGA AGAGATGCAA CCCATGCTGC AAGCTCACTT GGATGAGTTG 
ACAAGGGCCT ATCAGTTTGA TATAGCAAAA CAAACATGGC ACATTCAAAG CAATACCCCA 
CCTCCACAAC CCCCAGCTCC GCCATTAGGT TACGTGTCCG GAGCCTTGAT TTCTGATTTG 
GAGACAGATG TTCCAGATGA GGATGCTGAT GATGAAGAGG AACCATTAGA AATTCCCAGG 
CCCCTCAGAG CACTAGACCA GACACCTGGA TCCAGTATGG ACAATCTAGA CAGCTCTGTC 
ACAGGTTCAA TGGTAAATGG ATGGGGCTCT GCATCTGATG AGGATCGTAA CTTTTCTAGT 
CATAGATCTA GTGTAGGTAG CTCCTCAGAT GGCTCCATCT TTGCCAGCGG CAGCTTTGCA 
AAAGCATTGG TGGCAGCAGC AGACAAAGCT GGCTTTAGGC TGGATGGAAC CAGTCTTACG 
AGAACAGGAA AAGCCTTTAG CTCCTCTCAA AGGCAGCGGC CCACCAGCCC ATTTTCTACG 
GACAGTAACA CCAGTGCTGC CCAGAATCAA AGCCAGAGGC CTCGGCCCAC GAAAAAACAT 
AAGGGAGGAC GGATGGACCC ACAGCCAGTG TTACCTCATC GGAGGGAAGG GATGCCAGAT 
GAGGAGTCCC TGGTGCCCTA CAGCAAGCCC AGCTTCCCGT CTCCTGGCGG GCACAGCTCA 
TCAGGAACAT CTTCCTCTAA AGGGTCCACT GGCCCTCGGA AGGCTGACGT GCTGAGAGGC 
AGCCACCAGA GGAATGCCAA TGACCTTCTC GACATTGGAT ATGTGGGCTC AAATAGTCAA 
GGACAGTTTA CAGGTGAATT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo2 ( XM_017317013.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172502.1 Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OMu79341
Clone ID Related Accession (Same CDS sequence)	XM_017317013.1 , NM_001358493.1
Accession Version	XM_017317013.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4608bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	roundabout homolog 2 isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT 
CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG 
GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC 
CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA 
GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT 
ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG 
GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG 
CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT 
AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT 
TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC 
GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC 
CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT 
ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA 
CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA 
TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA 
GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT 
CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT 
GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG 
CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG 
GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC 
ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG 
GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT 
GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA 
GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT 
GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG 
GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT 
GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT 
CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG 
CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG 
CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT 
TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG 
GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT 
GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT 
GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGCCT TACTTTACAT CCCTGACTAC 
CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC 
ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA 
AAGAAGAAAA CTAAAAATTC TTCGAAAGCG CAGAAAAACA ACGGATCCAC TTGGGCTAAT 
GTCCCTCTAC CTCCTCCTCC CGTCCAGCCC CTTCCTGGTA CAGAGCTGGG CCACTATGCT 
GCGGAACAAG AAAATGGCTA TGACAGTGAC AGCTGGTGCC CACCATTACC GGTGCAAACA 
TACTTGCATC AGGGTATGGA AGATGAGCTG GAAGAAGACG AAGATCGGGT CCCAACACCT 
CCTGTTCGCG GCGTGGCCTC TTCACCAGCT ATCTCTTTTG GACAGCAGTC CACTGCCACT 
CTTACTCCAT CCCCACGGGA AGAGATGCAA CCCATGCTGC AAGCTCACTT GGATGAGTTG 
ACAAGGGCCT ATCAGTTTGA TATAGCAAAA CAAACATGGC ACATTCAAAG CAATACCCCA 
CCTCCACAAC CCCCAGCTCC GCCATTAGGT TACGTGTCCG GAGCCTTGAT TTCTGATTTG 
GAGACAGATG TTCCAGATGA GGATGCTGAT GATGAAGAGG AACCATTAGA AATTCCCAGG 
CCCCTCAGAG CACTAGACCA GACACCTGGA TCCAGTATGG ACAATCTAGA CAGCTCTGTC 
ACAGGAAAAG CCTTTAGCTC CTCTCAAAGG CAGCGGCCCA CCAGCCCATT TTCTACGGAC 
AGTAACACCA GTGCTGCCCA GAATCAAAGC CAGAGGCCTC GGCCCACGAA AAAACATAAG 
GGAGGACGGA TGGACCCACA GCCAGTGTTA CCTCATCGGA GGGAAGGGAT GCCAGATGAT 
CTTCCCCCAC CACCAGATCC CCCACCAGGT CAGGGTTTAA GGCAGCAGAT AGGCCTGAGC 
CAACACTCTG GTAACGTGGA AAACTCAACA GAGAGAAAAG GAAGCTCTCT AGAGAGACAA 
CAAGCAGCCA ACTTAGAAGA CACAAAGAGC TCATTGGATT GTCCAGCTAA AACTGTTCTA 
GAGTGGCAGC GTCAAACCCA GGACTGGATA AACTCTACAG AACGCCAAGA AGAAACTCGG 
AAAGCCCCAC ACAAACAAGG GGTTGGATCA GAGGAGTCCC TGGTGCCCTA CAGCAAGCCC 
AGCTTCCCGT CTCCTGGCGG GCACAGCTCA TCAGGAACAT CTTCCTCTAA AGGGTCCACT 
GGCCCTCGGA AGGCTGACGT GCTGAGAGGC AGCCACCAGA GGAATGCCAA TGACCTTCTC 
GACATTGGAT ATGTGGGCTC AAATAGTCAA GGACAGTTTA CAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172502.1
CDS	365..4972
Translation	MLSSEKVDKITRLELLFVLMLFFSGPTLGKLTRTPWKGKPWKGGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKAMSTDEGTYVCIAENRVGKVEASATLTVRVRPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPINQTLAVDGTALLKCKATGEPLPVISWLKEGFTFLGRDPRATIQDQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDMNDLPGPPSKPQVTDVSKNSVTLSWQPGTPGVLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVVVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQASTVWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSHNSTSISVSWDPPPADHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVVIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGGRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPNYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTTYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSVQQKTDLMGFGYSLPDQNKGNNALLYIPDYRLAEGLSNRMPHNQSQDFSTTSSHNSSERSGSLSGGKGGKKKKTKNSSKAQKNNGSTWANVPLPPPPVQPLPGTELGHYAAEQENGYDSDSWCPPLPVQTYLHQGMEDELEEDEDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNTPPPQPPAPPLGYVSGALISDLETDVPDEDADDEEEPLEIPRPLRALDQTPGSSMDNLDSSVTGKAFSSSQRQRPTSPFSTDSNTSAAQNQSQRPRPTKKHKGGRMDPQPVLPHRREGMPDDLPPPPDPPPGQGLRQQIGLSQHSGNVENSTERKGSSLERQQAANLEDTKSSLDCPAKTVLEWQRQTQDWINSTERQEETRKAPHKQGVGSEESLVPYSKPSFPSPGGHSSSGTSSSKGSTGPRKADVLRGSHQRNANDLLDIGYVGSNSQGQFTE

Target ORF information:

RefSeq Version	XM_017317013.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017317013.1

ORF Insert Sequence:

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT 
CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG 
GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC 
CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA 
GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT 
ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG 
GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG 
CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT 
AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT 
TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC 
GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC 
CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT 
ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA 
CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA 
TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA 
GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT 
CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT 
GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG 
CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG 
GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC 
ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG 
GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT 
GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA 
GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT 
GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG 
GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT 
GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT 
CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG 
CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG 
CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT 
TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG 
GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT 
GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT 
GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGCCT TACTTTACAT CCCTGACTAC 
CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC 
ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA 
AAGAAGAAAA CTAAAAATTC TTCGAAAGCG CAGAAAAACA ACGGATCCAC TTGGGCTAAT 
GTCCCTCTAC CTCCTCCTCC CGTCCAGCCC CTTCCTGGTA CAGAGCTGGG CCACTATGCT 
GCGGAACAAG AAAATGGCTA TGACAGTGAC AGCTGGTGCC CACCATTACC GGTGCAAACA 
TACTTGCATC AGGGTATGGA AGATGAGCTG GAAGAAGACG AAGATCGGGT CCCAACACCT 
CCTGTTCGCG GCGTGGCCTC TTCACCAGCT ATCTCTTTTG GACAGCAGTC CACTGCCACT 
CTTACTCCAT CCCCACGGGA AGAGATGCAA CCCATGCTGC AAGCTCACTT GGATGAGTTG 
ACAAGGGCCT ATCAGTTTGA TATAGCAAAA CAAACATGGC ACATTCAAAG CAATACCCCA 
CCTCCACAAC CCCCAGCTCC GCCATTAGGT TACGTGTCCG GAGCCTTGAT TTCTGATTTG 
GAGACAGATG TTCCAGATGA GGATGCTGAT GATGAAGAGG AACCATTAGA AATTCCCAGG 
CCCCTCAGAG CACTAGACCA GACACCTGGA TCCAGTATGG ACAATCTAGA CAGCTCTGTC 
ACAGGAAAAG CCTTTAGCTC CTCTCAAAGG CAGCGGCCCA CCAGCCCATT TTCTACGGAC 
AGTAACACCA GTGCTGCCCA GAATCAAAGC CAGAGGCCTC GGCCCACGAA AAAACATAAG 
GGAGGACGGA TGGACCCACA GCCAGTGTTA CCTCATCGGA GGGAAGGGAT GCCAGATGAT 
CTTCCCCCAC CACCAGATCC CCCACCAGGT CAGGGTTTAA GGCAGCAGAT AGGCCTGAGC 
CAACACTCTG GTAACGTGGA AAACTCAACA GAGAGAAAAG GAAGCTCTCT AGAGAGACAA 
CAAGCAGCCA ACTTAGAAGA CACAAAGAGC TCATTGGATT GTCCAGCTAA AACTGTTCTA 
GAGTGGCAGC GTCAAACCCA GGACTGGATA AACTCTACAG AACGCCAAGA AGAAACTCGG 
AAAGCCCCAC ACAAACAAGG GGTTGGATCA GAGGAGTCCC TGGTGCCCTA CAGCAAGCCC 
AGCTTCCCGT CTCCTGGCGG GCACAGCTCA TCAGGAACAT CTTCCTCTAA AGGGTCCACT 
GGCCCTCGGA AGGCTGACGT GCTGAGAGGC AGCCACCAGA GGAATGCCAA TGACCTTCTC 
GACATTGGAT ATGTGGGCTC AAATAGTCAA GGACAGTTTA CAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo2 ( XM_030249141.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105001.1 Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OMu86964
Clone ID Related Accession (Same CDS sequence)	XM_030249141.1
Accession Version	XM_030249141.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4518bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	roundabout homolog 2 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT 
CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG 
GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC 
CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA 
GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT 
ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG 
GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG 
CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT 
AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT 
TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC 
GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC 
CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT 
ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA 
CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA 
TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA 
GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT 
CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT 
GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG 
CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG 
GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC 
ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG 
GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT 
GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA 
GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT 
GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG 
GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT 
GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT 
CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG 
CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG 
CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT 
TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG 
GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT 
GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT 
GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGCCT TACTTTACAT CCCTGACTAC 
CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC 
ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA 
AAGAAGAAAA CTAAAAATTC TTCGAAAGCG CAGAAAAACA ACGGATCCAC TTGGGCTAAT 
GTCCCTCTAC CTCCTCCTCC CGTCCAGCCC CTTCCTGGTA CAGAGCTGGG CCACTATGCT 
GCGGAACAAG AAAATGGCTA TGACAGTGAC AGCTGGTGCC CACCATTACC GGTGCAAACA 
TACTTGCATC AGGGTATGGA AGATGAGCTG GAAGAAGACG AAGATCGGGT CCCAACACCT 
CCTGTTCGCG GCGTGGCCTC TTCACCAGCT ATCTCTTTTG GACAGCAGTC CACTGCCACT 
CTTACTCCAT CCCCACGGGA AGAGATGCAA CCCATGCTGC AAGCTCACTT GGATGAGTTG 
ACAAGGGCCT ATCAGTTTGA TATAGCAAAA CAAACATGGC ACATTCAAAG CAATACCCCA 
CCTCCACAAC CCCCAGCTCC GCCATTAGGT TACGTGTCCG GAGCCTTGAT TTCTGATTTG 
GAGACAGATG TTCCAGATGA GGATGCTGAT GATGAAGAGG AACCATTAGA AATTCCCAGG 
CCCCTCAGAG CACTAGACCA GACACCTGGA TCCAGTATGG ACAATCTAGA CAGCTCTGTC 
ACAGGTTCAA TGGTAAATGG ATGGGGCTCT GCATCTGATG AGGATCGTAA CTTTTCTAGT 
CATAGATCTA GTGTAGGTAG CTCCTCAGAT GGCTCCATCT TTGCCAGCGG CAGCTTTGCA 
AAAGCATTGG TGGCAGCAGC AGACAAAGCT GGCTTTAGGC TGGATGGAAC CAGTCTTACG 
AGAACAGGAA AAGCCTTTAG CTCCTCTCAA AGGCAGCGGC CCACCAGCCC ATTTTCTACG 
GACAGTAACA CCAGTGCTGC CCAGAATCAA AGCCAGAGGC CTCGGCCCAC GAAAAAACAT 
AAGGGAGGAC GGATGGACCC ACAGCCAGTG TTACCTCATC GGAGGGAAGG GATGCCAGAT 
GAGGAGTCCC TGGTGCCCTA CAGCAAGCCC AGCTTCCCGT CTCCTGGCGG GCACAGCTCA 
TCAGGAACAT CTTCCTCTAA AGGGTCCACT GGCCCTCGGA AGGCTGACGT GCTGAGAGGC 
AGCCACCAGA GGAATGCCAA TGACCTTCTC GACATTGGAT ATGTGGGCTC AAATAGTCAA 
GGACAGTTTA CAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105001.1
CDS	9440..13957
Translation	MLSSEKVDKITRLELLFVLMLFFSGPTLGKLTRTPWKGKPWKGGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKAMSTDEGTYVCIAENRVGKVEASATLTVRVRPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPINQTLAVDGTALLKCKATGEPLPVISWLKEGFTFLGRDPRATIQDQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDMNDLPGPPSKPQVTDVSKNSVTLSWQPGTPGVLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVVVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQASTVWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSHNSTSISVSWDPPPADHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVVIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGGRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPNYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTTYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSVQQKTDLMGFGYSLPDQNKGNNALLYIPDYRLAEGLSNRMPHNQSQDFSTTSSHNSSERSGSLSGGKGGKKKKTKNSSKAQKNNGSTWANVPLPPPPVQPLPGTELGHYAAEQENGYDSDSWCPPLPVQTYLHQGMEDELEEDEDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNTPPPQPPAPPLGYVSGALISDLETDVPDEDADDEEEPLEIPRPLRALDQTPGSSMDNLDSSVTGSMVNGWGSASDEDRNFSSHRSSVGSSSDGSIFASGSFAKALVAAADKAGFRLDGTSLTRTGKAFSSSQRQRPTSPFSTDSNTSAAQNQSQRPRPTKKHKGGRMDPQPVLPHRREGMPDEESLVPYSKPSFPSPGGHSSSGTSSSKGSTGPRKADVLRGSHQRNANDLLDIGYVGSNSQGQFTE

Target ORF information:

RefSeq Version	XM_030249141.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249141.1

ORF Insert Sequence:

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT 
CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG 
GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC 
CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA 
GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT 
ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG 
GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG 
CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT 
AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT 
TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC 
GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC 
CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT 
ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA 
CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA 
TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA 
GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT 
CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT 
GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG 
CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG 
GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC 
ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG 
GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT 
GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA 
GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT 
GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG 
GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT 
GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT 
CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG 
CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG 
CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT 
TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG 
GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT 
GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT 
GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGCCT TACTTTACAT CCCTGACTAC 
CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC 
ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA 
AAGAAGAAAA CTAAAAATTC TTCGAAAGCG CAGAAAAACA ACGGATCCAC TTGGGCTAAT 
GTCCCTCTAC CTCCTCCTCC CGTCCAGCCC CTTCCTGGTA CAGAGCTGGG CCACTATGCT 
GCGGAACAAG AAAATGGCTA TGACAGTGAC AGCTGGTGCC CACCATTACC GGTGCAAACA 
TACTTGCATC AGGGTATGGA AGATGAGCTG GAAGAAGACG AAGATCGGGT CCCAACACCT 
CCTGTTCGCG GCGTGGCCTC TTCACCAGCT ATCTCTTTTG GACAGCAGTC CACTGCCACT 
CTTACTCCAT CCCCACGGGA AGAGATGCAA CCCATGCTGC AAGCTCACTT GGATGAGTTG 
ACAAGGGCCT ATCAGTTTGA TATAGCAAAA CAAACATGGC ACATTCAAAG CAATACCCCA 
CCTCCACAAC CCCCAGCTCC GCCATTAGGT TACGTGTCCG GAGCCTTGAT TTCTGATTTG 
GAGACAGATG TTCCAGATGA GGATGCTGAT GATGAAGAGG AACCATTAGA AATTCCCAGG 
CCCCTCAGAG CACTAGACCA GACACCTGGA TCCAGTATGG ACAATCTAGA CAGCTCTGTC 
ACAGGTTCAA TGGTAAATGG ATGGGGCTCT GCATCTGATG AGGATCGTAA CTTTTCTAGT 
CATAGATCTA GTGTAGGTAG CTCCTCAGAT GGCTCCATCT TTGCCAGCGG CAGCTTTGCA 
AAAGCATTGG TGGCAGCAGC AGACAAAGCT GGCTTTAGGC TGGATGGAAC CAGTCTTACG 
AGAACAGGAA AAGCCTTTAG CTCCTCTCAA AGGCAGCGGC CCACCAGCCC ATTTTCTACG 
GACAGTAACA CCAGTGCTGC CCAGAATCAA AGCCAGAGGC CTCGGCCCAC GAAAAAACAT 
AAGGGAGGAC GGATGGACCC ACAGCCAGTG TTACCTCATC GGAGGGAAGG GATGCCAGAT 
GAGGAGTCCC TGGTGCCCTA CAGCAAGCCC AGCTTCCCGT CTCCTGGCGG GCACAGCTCA 
TCAGGAACAT CTTCCTCTAA AGGGTCCACT GGCCCTCGGA AGGCTGACGT GCTGAGAGGC 
AGCCACCAGA GGAATGCCAA TGACCTTCTC GACATTGGAT ATGTGGGCTC AAATAGTCAA 
GGACAGTTTA CAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo2 ( NM_175549.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_780758.3 Mus musculus roundabout guidance receptor 2 (Robo2), mRNA.

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CloneID	OMu15146
Clone ID Related Accession (Same CDS sequence)	NM_175549.4 , NM_175549.5
Accession Version	NM_175549.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4527bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-27
Organism	Mus musculus(House mouse)
Product	roundabout homolog 2 precursor
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ533876.1. On Jul 28, 2006 this sequence version replaced NM_175549.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ533876.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END##

ATGAATCCTC TGATGTTTAC ACTATTATTG CTCTTTGGAT TTCTCTGCAT TCAGATTGAT 
GGATCTCGTC TTCGTCAAGA AGACTTTCCC CCCAGGATCG TAGAACACCC TTCTGATGTC 
ATCGTCTCCA AGGGAGAGCC CACCACTCTG AACTGTAAAG CAGAGGGCCG ACCCACCCCC 
ACCATTGAAT GGTACAAGGA TGGTGAGAGG GTGGAGACAG ACAAGGATGA TCCCAGGTCC 
CACAGAATGC TTCTGCCCAG CGGATCTTTA TTCTTTTTGC GAATTGTTCA TGGGCGCAGA 
AGTAAACCGG ACGAAGGGAG TTACGTTTGT GTTGCAAGGA ACTATCTTGG TGAAGCAGTG 
AGTCGAAATG CATCTCTGGA AGTGGCATTA TTGCGAGATG ACTTCCGGCA AAACCCCACA 
GATGTGGTAG TCGCAGCTGG AGAGCCTGCA ATCTTGGAGT GCCAGCCACC ACGGGGACAC 
CCAGAACCAA CCATCTACTG GAAAAAGGAC AAAGTCCGAA TTGATGACAA GGAAGAGAGA 
ATAAGTATCC GTGGTGGGAA GCTGATGATC TCTAATACTA GGAAAAGCGA TGCTGGCATG 
TACACCTGTG TGGGAACCAA TATGGTGGGA GAAAGGGACA GCGACCCTGC AGAGCTCACT 
GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTGCT AGAGGAAGAA 
GCTGTAGAAT TCCGTTGTCA GGTCCAAGGA GATCCCCAGC CAACGGTGAG GTGGAAAAAA 
GATGATGCAG ACTTGCCGAG AGGAAGGTAT GATATCAAAG ATGACTACAC GCTGAGAATT 
AAAAAGGCCA TGAGTACTGA TGAAGGTACC TATGTGTGTA TTGCTGAGAA TCGGGTGGGA 
AAAGTGGAAG CCTCTGCTAC CCTCACTGTC CGAGCTCCTC CACAGTTTGT GGTTAGGCCA 
AGAGATCAGA TCGTTGCTCA AGGCCGAACA GTGACATTCC CCTGTGAAAC TAAAGGAAAC 
CCACAGCCAG CTGTTTTTTG GCAGAAAGAA GGCAGCCAGA ACCTACTTTT CCCGAATCAA 
CCTCAGCAGC CCAACAGCCG ATGTTCAGTG TCGCCCACGG GGGACCTCAC CATCACCAAC 
ATCCAGCGTT CAGATGCGGG TTACTACATC TGCCAGGCCC TAACCGTGGC AGGAAGCATT 
TTAGCTAAAG CACAGTTGGA AGTTACTGAC GTTTTGACAG ATAGACCTCC ACCCATAATC 
TTGCAAGGAC CAATAAACCA AACACTTGCA GTAGACGGTA CAGCATTGTT GAAGTGTAAA 
GCCACTGGTG AGCCTCTGCC TGTAATTAGC TGGCTAAAGG AGGGCTTTAC TTTTCTGGGG 
AGAGATCCAA GAGCCACGAT CCAAGACCAA GGAACACTGC AGATTAAGAA TTTACGGATA 
TCTGATACTG GCACTTATAC TTGTGTGGCT ACAAGTTCCA GTGGAGAGAC TTCCTGGAGT 
GCAGTGCTGG ATGTAACAGA ATCTGGAGCA ACAATCAGTA AAAATTATGA TATGAATGAC 
CTCCCGGGAC CACCATCCAA ACCTCAGGTC ACTGATGTTT CTAAGAACAG TGTCACCTTA 
TCCTGGCAGC CAGGTACACC TGGCGTTCTT CCTGCAAGCG CGTATATCAT TGAGGCTTTC 
AGCCAATCGG TGAGCAATAG CTGGCAGACA GTGGCAAACC ATGTTAAGAC AACTCTGTAT 
ACAGTAAGGG GGCTGAGGCC CAACACAATC TACTTGTTTA TGGTCAGAGC GATCAACCCA 
CAAGGTCTCA GTGATCCAAG TCCTATGTCG GATCCTGTAC GCACACAAGA TATCAGCCCC 
CCAGCACAAG GAGTGGACCA CAGACAGGTG CAGAAGGAAT TAGGTGATGT CGTTGTTCGT 
CTCCATAATC CAGTTGTCCT GACACCTACA ACTGTTCAAG TCACATGGAC GGTGGACCGA 
CAACCCCAGT TTATTCAGGG CTACAGAGTG ATGTACCGTC AGACTTCGGG ACTACAAGCC 
TCAACTGTGT GGCAGAATCT AGACGCCAAA GTCCCGACTG AGAGGAGTGC TGTCCTTGTG 
AATTTGAAAA AGGGGGTGAC TTATGAAATT AAAGTCCGGC CGTATTTTAA CGAGTTCCAA 
GGAATGGACA GTGAATCGAA AACAGTCCGA ACCACTGAGG AAGCCCCAAG TGCCCCTCCC 
CAGTCTGTCA CTGTGCTGAC AGTTGGAAGT CACAACAGCA CAAGCATCAG TGTTTCCTGG 
GATCCTCCAC CAGCCGACCA CCAGAATGGA ATTATTCAGG AATATAAGAT CTGGTGTCTG 
GGAAACGAAA CGCGATTCCA TATCAATAAA ACGGTGGATG CAGCCATTCG CTCTGTAGTA 
ATAGGTGGCT TGTTCCCTGG AATTCAGTAC CGGGTAGAAG TGGCAGCTAG CACAAGTGCA 
GGGGTTGGAG TAAAAAGTGA ACCACAGCCG ATAATAATTG GGGGACGTAA CGAAGTTGTC 
ATTACTGAAA ACAATAACAG CATCACTGAG CAAATCACGG ATGTCGTGAA GCAACCGGCA 
TTTATAGCTG GCATTGGTGG TGCCTGCTGG GTAATTCTGA TGGGTTTTAG CATCTGGTTG 
TACTGGAGAA GAAAGAAGAG AAAGGGACTC AGCAATTATG CTGTAACATT TCAAAGAGGA 
GATGGAGGAC TAATGAGCAA TGGGAGCCGT CCAGGTCTTC TAAATGCTGG CGATCCCAAT 
TACCCATGGC TTGCTGATTC TTGGCCAGCC ACGAGTTTGC CAGTGAACAA TAGCAATAGT 
GGCCCAAATG AAATTGGAAA TTTTGGGCGT GGAGATGTGC TGCCTCCGGT GCCAGGCCAA 
GGGGATAAAA CAGCGACCAT GCTCTCGGAT GGAGCCATTT ATAGCAGCAT TGACTTCACT 
ACCAAAACCA CTTACAACAG TTCCAGCCAA ATAACACAGG CCACCCCATA TGCCACTACA 
CAAATCCTGC ATTCAAACAG CATCCACGAA CTGGCAGTTG ATCTTCCTGA TCCACAGTGG 
AAAAGCTCAG TTCAACAGAA GACAGACCTC ATGGGATTTG GTTATTCGCT ACCTGATCAG 
AACAAGGGGA ACAACGCCTT ACTTTACATC CCTGACTACC GATTGGCTGA GGGATTGTCT 
AATAGAATGC CACACAACCA GTCACAGGAT TTCAGCACCA CCAGCTCTCA CAACAGCTCA 
GAAAGGAGTG GCAGTCTCTC AGGTGGGAAA GGTGGAAAAA AGAAGAAAAC TAAAAATTCT 
TCGAAAGCGC AGAAAAACAA CGGATCCACT TGGGCTAATG TCCCTCTACC TCCTCCTCCC 
GTCCAGCCCC TTCCTGGTAC AGAGCTGGGC CACTATGCTG CGGAACAAGA AAATGGCTAT 
GACAGTGACA GCTGGTGCCC ACCATTACCG GTGCAAACAT ACTTGCATCA GGGTATGGAA 
GATGAGCTGG AAGAAGACGA AGATCGGGTC CCAACACCTC CTGTTCGCGG CGTGGCCTCT 
TCACCAGCTA TCTCTTTTGG ACAGCAGTCC ACTGCCACTC TTACTCCATC CCCACGGGAA 
GAGATGCAAC CCATGCTGCA AGCTCACTTG GATGAGTTGA CAAGGGCCTA TCAGTTTGAT 
ATAGCAAAAC AAACATGGCA CATTCAAAGC AATACCCCAC CTCCACAACC CCCAGCTCCG 
CCATTAGGTT ACGTGTCCGG AGCCTTGATT TCTGATTTGG AGACAGATGT TCCAGATGAG 
GATGCTGATG ATGAAGAGGA ACCATTAGAA ATTCCCAGGC CCCTCAGAGC ACTAGACCAG 
ACACCTGGAT CCAGTATGGA CAATCTAGAC AGCTCTGTCA CAGGAAAAGC CTTTAGCTCC 
TCTCAAAGGC AGCGGCCCAC CAGCCCATTT TCTACGGACA GTAACACCAG TGCTGCCCAG 
AATCAAAGCC AGAGGCCTCG GCCCACGAAA AAACATAAGG GAGGACGGAT GGACCCACAG 
CCAGTGTTAC CTCATCGGAG GGAAGGGATG CCAGATGATC TTCCCCCACC ACCAGATCCC 
CCACCAGGTC AGGGTTTAAG GCAGCAGATA GGCCTGAGCC AACACTCTGG TAACGTGGAA 
AACTCAACAG AGAGAAAAGG AAGCTCTCTA GAGAGACAAC AAGCAGCCAA CTTAGAAGAC 
ACAAAGAGCT CATTGGATTG TCCAGCTAAA ACTGTTCTAG AGTGGCAGCG TCAAACCCAG 
GACTGGATAA ACTCTACAGA ACGCCAAGAA GAAACTCGGA AAGCCCCACA CAAACAAGGG 
GTTGGATCAG AGGAGTCCCT GGTGCCCTAC AGCAAGCCCA GCTTCCCGTC TCCTGGCGGG 
CACAGCTCAT CAGGAACATC TTCCTCTAAA GGGTCCACTG GCCCTCGGAA GGCTGACGTG 
CTGAGAGGCA GCCACCAGAG GAATGCCAAT GACCTTCTCG ACATTGGATA TGTGGGCTCA 
AATAGTCAAG GACAGTTTAC AGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_780758.3
CDS	1..4527
Translation	MNPLMFTLLLLFGFLCIQIDGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKAMSTDEGTYVCIAENRVGKVEASATLTVRAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPINQTLAVDGTALLKCKATGEPLPVISWLKEGFTFLGRDPRATIQDQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDMNDLPGPPSKPQVTDVSKNSVTLSWQPGTPGVLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVVVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQASTVWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSHNSTSISVSWDPPPADHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVVIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGGRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPNYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTTYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSVQQKTDLMGFGYSLPDQNKGNNALLYIPDYRLAEGLSNRMPHNQSQDFSTTSSHNSSERSGSLSGGKGGKKKKTKNSSKAQKNNGSTWANVPLPPPPVQPLPGTELGHYAAEQENGYDSDSWCPPLPVQTYLHQGMEDELEEDEDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNTPPPQPPAPPLGYVSGALISDLETDVPDEDADDEEEPLEIPRPLRALDQTPGSSMDNLDSSVTGKAFSSSQRQRPTSPFSTDSNTSAAQNQSQRPRPTKKHKGGRMDPQPVLPHRREGMPDDLPPPPDPPPGQGLRQQIGLSQHSGNVENSTERKGSSLERQQAANLEDTKSSLDCPAKTVLEWQRQTQDWINSTERQEETRKAPHKQGVGSEESLVPYSKPSFPSPGGHSSSGTSSSKGSTGPRKADVLRGSHQRNANDLLDIGYVGSNSQGQFTE

Target ORF information:

RefSeq Version	NM_175549.4
Organism	Mus musculus(House mouse)
Definition	Mus musculus roundabout guidance receptor 2 (Robo2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175549.4

ORF Insert Sequence:

ATGAATCCTC TGATGTTTAC ACTATTATTG CTCTTTGGAT TTCTCTGCAT TCAGATTGAT 
GGATCTCGTC TTCGTCAAGA AGACTTTCCC CCCAGGATCG TAGAACACCC TTCTGATGTC 
ATCGTCTCCA AGGGAGAGCC CACCACTCTG AACTGTAAAG CAGAGGGCCG ACCCACCCCC 
ACCATTGAAT GGTACAAGGA TGGTGAGAGG GTGGAGACAG ACAAGGATGA TCCCAGGTCC 
CACAGAATGC TTCTGCCCAG CGGATCTTTA TTCTTTTTGC GAATTGTTCA TGGGCGCAGA 
AGTAAACCGG ACGAAGGGAG TTACGTTTGT GTTGCAAGGA ACTATCTTGG TGAAGCAGTG 
AGTCGAAATG CATCTCTGGA AGTGGCATTA TTGCGAGATG ACTTCCGGCA AAACCCCACA 
GATGTGGTAG TCGCAGCTGG AGAGCCTGCA ATCTTGGAGT GCCAGCCACC ACGGGGACAC 
CCAGAACCAA CCATCTACTG GAAAAAGGAC AAAGTCCGAA TTGATGACAA GGAAGAGAGA 
ATAAGTATCC GTGGTGGGAA GCTGATGATC TCTAATACTA GGAAAAGCGA TGCTGGCATG 
TACACCTGTG TGGGAACCAA TATGGTGGGA GAAAGGGACA GCGACCCTGC AGAGCTCACT 
GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTGCT AGAGGAAGAA 
GCTGTAGAAT TCCGTTGTCA GGTCCAAGGA GATCCCCAGC CAACGGTGAG GTGGAAAAAA 
GATGATGCAG ACTTGCCGAG AGGAAGGTAT GATATCAAAG ATGACTACAC GCTGAGAATT 
AAAAAGGCCA TGAGTACTGA TGAAGGTACC TATGTGTGTA TTGCTGAGAA TCGGGTGGGA 
AAAGTGGAAG CCTCTGCTAC CCTCACTGTC CGAGCTCCTC CACAGTTTGT GGTTAGGCCA 
AGAGATCAGA TCGTTGCTCA AGGCCGAACA GTGACATTCC CCTGTGAAAC TAAAGGAAAC 
CCACAGCCAG CTGTTTTTTG GCAGAAAGAA GGCAGCCAGA ACCTACTTTT CCCGAATCAA 
CCTCAGCAGC CCAACAGCCG ATGTTCAGTG TCGCCCACGG GGGACCTCAC CATCACCAAC 
ATCCAGCGTT CAGATGCGGG TTACTACATC TGCCAGGCCC TAACCGTGGC AGGAAGCATT 
TTAGCTAAAG CACAGTTGGA AGTTACTGAC GTTTTGACAG ATAGACCTCC ACCCATAATC 
TTGCAAGGAC CAATAAACCA AACACTTGCA GTAGACGGTA CAGCATTGTT GAAGTGTAAA 
GCCACTGGTG AGCCTCTGCC TGTAATTAGC TGGCTAAAGG AGGGCTTTAC TTTTCTGGGG 
AGAGATCCAA GAGCCACGAT CCAAGACCAA GGAACACTGC AGATTAAGAA TTTACGGATA 
TCTGATACTG GCACTTATAC TTGTGTGGCT ACAAGTTCCA GTGGAGAGAC TTCCTGGAGT 
GCAGTGCTGG ATGTAACAGA ATCTGGAGCA ACAATCAGTA AAAATTATGA TATGAATGAC 
CTCCCGGGAC CACCATCCAA ACCTCAGGTC ACTGATGTTT CTAAGAACAG TGTCACCTTA 
TCCTGGCAGC CAGGTACACC TGGCGTTCTT CCTGCAAGCG CGTATATCAT TGAGGCTTTC 
AGCCAATCGG TGAGCAATAG CTGGCAGACA GTGGCAAACC ATGTTAAGAC AACTCTGTAT 
ACAGTAAGGG GGCTGAGGCC CAACACAATC TACTTGTTTA TGGTCAGAGC GATCAACCCA 
CAAGGTCTCA GTGATCCAAG TCCTATGTCG GATCCTGTAC GCACACAAGA TATCAGCCCC 
CCAGCACAAG GAGTGGACCA CAGACAGGTG CAGAAGGAAT TAGGTGATGT CGTTGTTCGT 
CTCCATAATC CAGTTGTCCT GACACCTACA ACTGTTCAAG TCACATGGAC GGTGGACCGA 
CAACCCCAGT TTATTCAGGG CTACAGAGTG ATGTACCGTC AGACTTCGGG ACTACAAGCC 
TCAACTGTGT GGCAGAATCT AGACGCCAAA GTCCCGACTG AGAGGAGTGC TGTCCTTGTG 
AATTTGAAAA AGGGGGTGAC TTATGAAATT AAAGTCCGGC CGTATTTTAA CGAGTTCCAA 
GGAATGGACA GTGAATCGAA AACAGTCCGA ACCACTGAGG AAGCCCCAAG TGCCCCTCCC 
CAGTCTGTCA CTGTGCTGAC AGTTGGAAGT CACAACAGCA CAAGCATCAG TGTTTCCTGG 
GATCCTCCAC CAGCCGACCA CCAGAATGGA ATTATTCAGG AATATAAGAT CTGGTGTCTG 
GGAAACGAAA CGCGATTCCA TATCAATAAA ACGGTGGATG CAGCCATTCG CTCTGTAGTA 
ATAGGTGGCT TGTTCCCTGG AATTCAGTAC CGGGTAGAAG TGGCAGCTAG CACAAGTGCA 
GGGGTTGGAG TAAAAAGTGA ACCACAGCCG ATAATAATTG GGGGACGTAA CGAAGTTGTC 
ATTACTGAAA ACAATAACAG CATCACTGAG CAAATCACGG ATGTCGTGAA GCAACCGGCA 
TTTATAGCTG GCATTGGTGG TGCCTGCTGG GTAATTCTGA TGGGTTTTAG CATCTGGTTG 
TACTGGAGAA GAAAGAAGAG AAAGGGACTC AGCAATTATG CTGTAACATT TCAAAGAGGA 
GATGGAGGAC TAATGAGCAA TGGGAGCCGT CCAGGTCTTC TAAATGCTGG CGATCCCAAT 
TACCCATGGC TTGCTGATTC TTGGCCAGCC ACGAGTTTGC CAGTGAACAA TAGCAATAGT 
GGCCCAAATG AAATTGGAAA TTTTGGGCGT GGAGATGTGC TGCCTCCGGT GCCAGGCCAA 
GGGGATAAAA CAGCGACCAT GCTCTCGGAT GGAGCCATTT ATAGCAGCAT TGACTTCACT 
ACCAAAACCA CTTACAACAG TTCCAGCCAA ATAACACAGG CCACCCCATA TGCCACTACA 
CAAATCCTGC ATTCAAACAG CATCCACGAA CTGGCAGTTG ATCTTCCTGA TCCACAGTGG 
AAAAGCTCAG TTCAACAGAA GACAGACCTC ATGGGATTTG GTTATTCGCT ACCTGATCAG 
AACAAGGGGA ACAACGCCTT ACTTTACATC CCTGACTACC GATTGGCTGA GGGATTGTCT 
AATAGAATGC CACACAACCA GTCACAGGAT TTCAGCACCA CCAGCTCTCA CAACAGCTCA 
GAAAGGAGTG GCAGTCTCTC AGGTGGGAAA GGTGGAAAAA AGAAGAAAAC TAAAAATTCT 
TCGAAAGCGC AGAAAAACAA CGGATCCACT TGGGCTAATG TCCCTCTACC TCCTCCTCCC 
GTCCAGCCCC TTCCTGGTAC AGAGCTGGGC CACTATGCTG CGGAACAAGA AAATGGCTAT 
GACAGTGACA GCTGGTGCCC ACCATTACCG GTGCAAACAT ACTTGCATCA GGGTATGGAA 
GATGAGCTGG AAGAAGACGA AGATCGGGTC CCAACACCTC CTGTTCGCGG CGTGGCCTCT 
TCACCAGCTA TCTCTTTTGG ACAGCAGTCC ACTGCCACTC TTACTCCATC CCCACGGGAA 
GAGATGCAAC CCATGCTGCA AGCTCACTTG GATGAGTTGA CAAGGGCCTA TCAGTTTGAT 
ATAGCAAAAC AAACATGGCA CATTCAAAGC AATACCCCAC CTCCACAACC CCCAGCTCCG 
CCATTAGGTT ACGTGTCCGG AGCCTTGATT TCTGATTTGG AGACAGATGT TCCAGATGAG 
GATGCTGATG ATGAAGAGGA ACCATTAGAA ATTCCCAGGC CCCTCAGAGC ACTAGACCAG 
ACACCTGGAT CCAGTATGGA CAATCTAGAC AGCTCTGTCA CAGGAAAAGC CTTTAGCTCC 
TCTCAAAGGC AGCGGCCCAC CAGCCCATTT TCTACGGACA GTAACACCAG TGCTGCCCAG 
AATCAAAGCC AGAGGCCTCG GCCCACGAAA AAACATAAGG GAGGACGGAT GGACCCACAG 
CCAGTGTTAC CTCATCGGAG GGAAGGGATG CCAGATGATC TTCCCCCACC ACCAGATCCC 
CCACCAGGTC AGGGTTTAAG GCAGCAGATA GGCCTGAGCC AACACTCTGG TAACGTGGAA 
AACTCAACAG AGAGAAAAGG AAGCTCTCTA GAGAGACAAC AAGCAGCCAA CTTAGAAGAC 
ACAAAGAGCT CATTGGATTG TCCAGCTAAA ACTGTTCTAG AGTGGCAGCG TCAAACCCAG 
GACTGGATAA ACTCTACAGA ACGCCAAGAA GAAACTCGGA AAGCCCCACA CAAACAAGGG 
GTTGGATCAG AGGAGTCCCT GGTGCCCTAC AGCAAGCCCA GCTTCCCGTC TCCTGGCGGG 
CACAGCTCAT CAGGAACATC TTCCTCTAAA GGGTCCACTG GCCCTCGGAA GGCTGACGTG 
CTGAGAGGCA GCCACCAGAG GAATGCCAAT GACCTTCTCG ACATTGGATA TGTGGGCTCA 
AATAGTCAAG GACAGTTTAC AGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo2 ( NM_175549.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_780758.3 Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant 5, mRNA.

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CloneID	OMu15146
Clone ID Related Accession (Same CDS sequence)	NM_175549.4 , NM_175549.5
Accession Version	NM_175549.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4527bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-22
Organism	Mus musculus(house mouse)
Product	roundabout homolog 2 isoform 5 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC158234.2, AC112155.3, AC134452.4, AC122049.2 and AC162374.4. On Dec 1, 2017 this sequence version replaced NM_175549.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ533876.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END##

ATGAATCCTC TGATGTTTAC ACTATTATTG CTCTTTGGAT TTCTCTGCAT TCAGATTGAT 
GGATCTCGTC TTCGTCAAGA AGACTTTCCC CCCAGGATCG TAGAACACCC TTCTGATGTC 
ATCGTCTCCA AGGGAGAGCC CACCACTCTG AACTGTAAAG CAGAGGGCCG ACCCACCCCC 
ACCATTGAAT GGTACAAGGA TGGTGAGAGG GTGGAGACAG ACAAGGATGA TCCCAGGTCC 
CACAGAATGC TTCTGCCCAG CGGATCTTTA TTCTTTTTGC GAATTGTTCA TGGGCGCAGA 
AGTAAACCGG ACGAAGGGAG TTACGTTTGT GTTGCAAGGA ACTATCTTGG TGAAGCAGTG 
AGTCGAAATG CATCTCTGGA AGTGGCATTA TTGCGAGATG ACTTCCGGCA AAACCCCACA 
GATGTGGTAG TCGCAGCTGG AGAGCCTGCA ATCTTGGAGT GCCAGCCACC ACGGGGACAC 
CCAGAACCAA CCATCTACTG GAAAAAGGAC AAAGTCCGAA TTGATGACAA GGAAGAGAGA 
ATAAGTATCC GTGGTGGGAA GCTGATGATC TCTAATACTA GGAAAAGCGA TGCTGGCATG 
TACACCTGTG TGGGAACCAA TATGGTGGGA GAAAGGGACA GCGACCCTGC AGAGCTCACT 
GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTGCT AGAGGAAGAA 
GCTGTAGAAT TCCGTTGTCA GGTCCAAGGA GATCCCCAGC CAACGGTGAG GTGGAAAAAA 
GATGATGCAG ACTTGCCGAG AGGAAGGTAT GATATCAAAG ATGACTACAC GCTGAGAATT 
AAAAAGGCCA TGAGTACTGA TGAAGGTACC TATGTGTGTA TTGCTGAGAA TCGGGTGGGA 
AAAGTGGAAG CCTCTGCTAC CCTCACTGTC CGAGCTCCTC CACAGTTTGT GGTTAGGCCA 
AGAGATCAGA TCGTTGCTCA AGGCCGAACA GTGACATTCC CCTGTGAAAC TAAAGGAAAC 
CCACAGCCAG CTGTTTTTTG GCAGAAAGAA GGCAGCCAGA ACCTACTTTT CCCGAATCAA 
CCTCAGCAGC CCAACAGCCG ATGTTCAGTG TCGCCCACGG GGGACCTCAC CATCACCAAC 
ATCCAGCGTT CAGATGCGGG TTACTACATC TGCCAGGCCC TAACCGTGGC AGGAAGCATT 
TTAGCTAAAG CACAGTTGGA AGTTACTGAC GTTTTGACAG ATAGACCTCC ACCCATAATC 
TTGCAAGGAC CAATAAACCA AACACTTGCA GTAGACGGTA CAGCATTGTT GAAGTGTAAA 
GCCACTGGTG AGCCTCTGCC TGTAATTAGC TGGCTAAAGG AGGGCTTTAC TTTTCTGGGG 
AGAGATCCAA GAGCCACGAT CCAAGACCAA GGAACACTGC AGATTAAGAA TTTACGGATA 
TCTGATACTG GCACTTATAC TTGTGTGGCT ACAAGTTCCA GTGGAGAGAC TTCCTGGAGT 
GCAGTGCTGG ATGTAACAGA ATCTGGAGCA ACAATCAGTA AAAATTATGA TATGAATGAC 
CTCCCGGGAC CACCATCCAA ACCTCAGGTC ACTGATGTTT CTAAGAACAG TGTCACCTTA 
TCCTGGCAGC CAGGTACACC TGGCGTTCTT CCTGCAAGCG CGTATATCAT TGAGGCTTTC 
AGCCAATCGG TGAGCAATAG CTGGCAGACA GTGGCAAACC ATGTTAAGAC AACTCTGTAT 
ACAGTAAGGG GGCTGAGGCC CAACACAATC TACTTGTTTA TGGTCAGAGC GATCAACCCA 
CAAGGTCTCA GTGATCCAAG TCCTATGTCG GATCCTGTAC GCACACAAGA TATCAGCCCC 
CCAGCACAAG GAGTGGACCA CAGACAGGTG CAGAAGGAAT TAGGTGATGT CGTTGTTCGT 
CTCCATAATC CAGTTGTCCT GACACCTACA ACTGTTCAAG TCACATGGAC GGTGGACCGA 
CAACCCCAGT TTATTCAGGG CTACAGAGTG ATGTACCGTC AGACTTCGGG ACTACAAGCC 
TCAACTGTGT GGCAGAATCT AGACGCCAAA GTCCCGACTG AGAGGAGTGC TGTCCTTGTG 
AATTTGAAAA AGGGGGTGAC TTATGAAATT AAAGTCCGGC CGTATTTTAA CGAGTTCCAA 
GGAATGGACA GTGAATCGAA AACAGTCCGA ACCACTGAGG AAGCCCCAAG TGCCCCTCCC 
CAGTCTGTCA CTGTGCTGAC AGTTGGAAGT CACAACAGCA CAAGCATCAG TGTTTCCTGG 
GATCCTCCAC CAGCCGACCA CCAGAATGGA ATTATTCAGG AATATAAGAT CTGGTGTCTG 
GGAAACGAAA CGCGATTCCA TATCAATAAA ACGGTGGATG CAGCCATTCG CTCTGTAGTA 
ATAGGTGGCT TGTTCCCTGG AATTCAGTAC CGGGTAGAAG TGGCAGCTAG CACAAGTGCA 
GGGGTTGGAG TAAAAAGTGA ACCACAGCCG ATAATAATTG GGGGACGTAA CGAAGTTGTC 
ATTACTGAAA ACAATAACAG CATCACTGAG CAAATCACGG ATGTCGTGAA GCAACCGGCA 
TTTATAGCTG GCATTGGTGG TGCCTGCTGG GTAATTCTGA TGGGTTTTAG CATCTGGTTG 
TACTGGAGAA GAAAGAAGAG AAAGGGACTC AGCAATTATG CTGTAACATT TCAAAGAGGA 
GATGGAGGAC TAATGAGCAA TGGGAGCCGT CCAGGTCTTC TAAATGCTGG CGATCCCAAT 
TACCCATGGC TTGCTGATTC TTGGCCAGCC ACGAGTTTGC CAGTGAACAA TAGCAATAGT 
GGCCCAAATG AAATTGGAAA TTTTGGGCGT GGAGATGTGC TGCCTCCGGT GCCAGGCCAA 
GGGGATAAAA CAGCGACCAT GCTCTCGGAT GGAGCCATTT ATAGCAGCAT TGACTTCACT 
ACCAAAACCA CTTACAACAG TTCCAGCCAA ATAACACAGG CCACCCCATA TGCCACTACA 
CAAATCCTGC ATTCAAACAG CATCCACGAA CTGGCAGTTG ATCTTCCTGA TCCACAGTGG 
AAAAGCTCAG TTCAACAGAA GACAGACCTC ATGGGATTTG GTTATTCGCT ACCTGATCAG 
AACAAGGGGA ACAACGCCTT ACTTTACATC CCTGACTACC GATTGGCTGA GGGATTGTCT 
AATAGAATGC CACACAACCA GTCACAGGAT TTCAGCACCA CCAGCTCTCA CAACAGCTCA 
GAAAGGAGTG GCAGTCTCTC AGGTGGGAAA GGTGGAAAAA AGAAGAAAAC TAAAAATTCT 
TCGAAAGCGC AGAAAAACAA CGGATCCACT TGGGCTAATG TCCCTCTACC TCCTCCTCCC 
GTCCAGCCCC TTCCTGGTAC AGAGCTGGGC CACTATGCTG CGGAACAAGA AAATGGCTAT 
GACAGTGACA GCTGGTGCCC ACCATTACCG GTGCAAACAT ACTTGCATCA GGGTATGGAA 
GATGAGCTGG AAGAAGACGA AGATCGGGTC CCAACACCTC CTGTTCGCGG CGTGGCCTCT 
TCACCAGCTA TCTCTTTTGG ACAGCAGTCC ACTGCCACTC TTACTCCATC CCCACGGGAA 
GAGATGCAAC CCATGCTGCA AGCTCACTTG GATGAGTTGA CAAGGGCCTA TCAGTTTGAT 
ATAGCAAAAC AAACATGGCA CATTCAAAGC AATACCCCAC CTCCACAACC CCCAGCTCCG 
CCATTAGGTT ACGTGTCCGG AGCCTTGATT TCTGATTTGG AGACAGATGT TCCAGATGAG 
GATGCTGATG ATGAAGAGGA ACCATTAGAA ATTCCCAGGC CCCTCAGAGC ACTAGACCAG 
ACACCTGGAT CCAGTATGGA CAATCTAGAC AGCTCTGTCA CAGGAAAAGC CTTTAGCTCC 
TCTCAAAGGC AGCGGCCCAC CAGCCCATTT TCTACGGACA GTAACACCAG TGCTGCCCAG 
AATCAAAGCC AGAGGCCTCG GCCCACGAAA AAACATAAGG GAGGACGGAT GGACCCACAG 
CCAGTGTTAC CTCATCGGAG GGAAGGGATG CCAGATGATC TTCCCCCACC ACCAGATCCC 
CCACCAGGTC AGGGTTTAAG GCAGCAGATA GGCCTGAGCC AACACTCTGG TAACGTGGAA 
AACTCAACAG AGAGAAAAGG AAGCTCTCTA GAGAGACAAC AAGCAGCCAA CTTAGAAGAC 
ACAAAGAGCT CATTGGATTG TCCAGCTAAA ACTGTTCTAG AGTGGCAGCG TCAAACCCAG 
GACTGGATAA ACTCTACAGA ACGCCAAGAA GAAACTCGGA AAGCCCCACA CAAACAAGGG 
GTTGGATCAG AGGAGTCCCT GGTGCCCTAC AGCAAGCCCA GCTTCCCGTC TCCTGGCGGG 
CACAGCTCAT CAGGAACATC TTCCTCTAAA GGGTCCACTG GCCCTCGGAA GGCTGACGTG 
CTGAGAGGCA GCCACCAGAG GAATGCCAAT GACCTTCTCG ACATTGGATA TGTGGGCTCA 
AATAGTCAAG GACAGTTTAC AGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_780758.3
CDS	914..5440
Translation	MNPLMFTLLLLFGFLCIQIDGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKAMSTDEGTYVCIAENRVGKVEASATLTVRAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPINQTLAVDGTALLKCKATGEPLPVISWLKEGFTFLGRDPRATIQDQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDMNDLPGPPSKPQVTDVSKNSVTLSWQPGTPGVLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVVVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQASTVWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSHNSTSISVSWDPPPADHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVVIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGGRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPNYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTTYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSVQQKTDLMGFGYSLPDQNKGNNALLYIPDYRLAEGLSNRMPHNQSQDFSTTSSHNSSERSGSLSGGKGGKKKKTKNSSKAQKNNGSTWANVPLPPPPVQPLPGTELGHYAAEQENGYDSDSWCPPLPVQTYLHQGMEDELEEDEDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNTPPPQPPAPPLGYVSGALISDLETDVPDEDADDEEEPLEIPRPLRALDQTPGSSMDNLDSSVTGKAFSSSQRQRPTSPFSTDSNTSAAQNQSQRPRPTKKHKGGRMDPQPVLPHRREGMPDDLPPPPDPPPGQGLRQQIGLSQHSGNVENSTERKGSSLERQQAANLEDTKSSLDCPAKTVLEWQRQTQDWINSTERQEETRKAPHKQGVGSEESLVPYSKPSFPSPGGHSSSGTSSSKGSTGPRKADVLRGSHQRNANDLLDIGYVGSNSQGQFTE

Target ORF information:

RefSeq Version	NM_175549.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175549.5

ORF Insert Sequence:

ATGAATCCTC TGATGTTTAC ACTATTATTG CTCTTTGGAT TTCTCTGCAT TCAGATTGAT 
GGATCTCGTC TTCGTCAAGA AGACTTTCCC CCCAGGATCG TAGAACACCC TTCTGATGTC 
ATCGTCTCCA AGGGAGAGCC CACCACTCTG AACTGTAAAG CAGAGGGCCG ACCCACCCCC 
ACCATTGAAT GGTACAAGGA TGGTGAGAGG GTGGAGACAG ACAAGGATGA TCCCAGGTCC 
CACAGAATGC TTCTGCCCAG CGGATCTTTA TTCTTTTTGC GAATTGTTCA TGGGCGCAGA 
AGTAAACCGG ACGAAGGGAG TTACGTTTGT GTTGCAAGGA ACTATCTTGG TGAAGCAGTG 
AGTCGAAATG CATCTCTGGA AGTGGCATTA TTGCGAGATG ACTTCCGGCA AAACCCCACA 
GATGTGGTAG TCGCAGCTGG AGAGCCTGCA ATCTTGGAGT GCCAGCCACC ACGGGGACAC 
CCAGAACCAA CCATCTACTG GAAAAAGGAC AAAGTCCGAA TTGATGACAA GGAAGAGAGA 
ATAAGTATCC GTGGTGGGAA GCTGATGATC TCTAATACTA GGAAAAGCGA TGCTGGCATG 
TACACCTGTG TGGGAACCAA TATGGTGGGA GAAAGGGACA GCGACCCTGC AGAGCTCACT 
GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTGCT AGAGGAAGAA 
GCTGTAGAAT TCCGTTGTCA GGTCCAAGGA GATCCCCAGC CAACGGTGAG GTGGAAAAAA 
GATGATGCAG ACTTGCCGAG AGGAAGGTAT GATATCAAAG ATGACTACAC GCTGAGAATT 
AAAAAGGCCA TGAGTACTGA TGAAGGTACC TATGTGTGTA TTGCTGAGAA TCGGGTGGGA 
AAAGTGGAAG CCTCTGCTAC CCTCACTGTC CGAGCTCCTC CACAGTTTGT GGTTAGGCCA 
AGAGATCAGA TCGTTGCTCA AGGCCGAACA GTGACATTCC CCTGTGAAAC TAAAGGAAAC 
CCACAGCCAG CTGTTTTTTG GCAGAAAGAA GGCAGCCAGA ACCTACTTTT CCCGAATCAA 
CCTCAGCAGC CCAACAGCCG ATGTTCAGTG TCGCCCACGG GGGACCTCAC CATCACCAAC 
ATCCAGCGTT CAGATGCGGG TTACTACATC TGCCAGGCCC TAACCGTGGC AGGAAGCATT 
TTAGCTAAAG CACAGTTGGA AGTTACTGAC GTTTTGACAG ATAGACCTCC ACCCATAATC 
TTGCAAGGAC CAATAAACCA AACACTTGCA GTAGACGGTA CAGCATTGTT GAAGTGTAAA 
GCCACTGGTG AGCCTCTGCC TGTAATTAGC TGGCTAAAGG AGGGCTTTAC TTTTCTGGGG 
AGAGATCCAA GAGCCACGAT CCAAGACCAA GGAACACTGC AGATTAAGAA TTTACGGATA 
TCTGATACTG GCACTTATAC TTGTGTGGCT ACAAGTTCCA GTGGAGAGAC TTCCTGGAGT 
GCAGTGCTGG ATGTAACAGA ATCTGGAGCA ACAATCAGTA AAAATTATGA TATGAATGAC 
CTCCCGGGAC CACCATCCAA ACCTCAGGTC ACTGATGTTT CTAAGAACAG TGTCACCTTA 
TCCTGGCAGC CAGGTACACC TGGCGTTCTT CCTGCAAGCG CGTATATCAT TGAGGCTTTC 
AGCCAATCGG TGAGCAATAG CTGGCAGACA GTGGCAAACC ATGTTAAGAC AACTCTGTAT 
ACAGTAAGGG GGCTGAGGCC CAACACAATC TACTTGTTTA TGGTCAGAGC GATCAACCCA 
CAAGGTCTCA GTGATCCAAG TCCTATGTCG GATCCTGTAC GCACACAAGA TATCAGCCCC 
CCAGCACAAG GAGTGGACCA CAGACAGGTG CAGAAGGAAT TAGGTGATGT CGTTGTTCGT 
CTCCATAATC CAGTTGTCCT GACACCTACA ACTGTTCAAG TCACATGGAC GGTGGACCGA 
CAACCCCAGT TTATTCAGGG CTACAGAGTG ATGTACCGTC AGACTTCGGG ACTACAAGCC 
TCAACTGTGT GGCAGAATCT AGACGCCAAA GTCCCGACTG AGAGGAGTGC TGTCCTTGTG 
AATTTGAAAA AGGGGGTGAC TTATGAAATT AAAGTCCGGC CGTATTTTAA CGAGTTCCAA 
GGAATGGACA GTGAATCGAA AACAGTCCGA ACCACTGAGG AAGCCCCAAG TGCCCCTCCC 
CAGTCTGTCA CTGTGCTGAC AGTTGGAAGT CACAACAGCA CAAGCATCAG TGTTTCCTGG 
GATCCTCCAC CAGCCGACCA CCAGAATGGA ATTATTCAGG AATATAAGAT CTGGTGTCTG 
GGAAACGAAA CGCGATTCCA TATCAATAAA ACGGTGGATG CAGCCATTCG CTCTGTAGTA 
ATAGGTGGCT TGTTCCCTGG AATTCAGTAC CGGGTAGAAG TGGCAGCTAG CACAAGTGCA 
GGGGTTGGAG TAAAAAGTGA ACCACAGCCG ATAATAATTG GGGGACGTAA CGAAGTTGTC 
ATTACTGAAA ACAATAACAG CATCACTGAG CAAATCACGG ATGTCGTGAA GCAACCGGCA 
TTTATAGCTG GCATTGGTGG TGCCTGCTGG GTAATTCTGA TGGGTTTTAG CATCTGGTTG 
TACTGGAGAA GAAAGAAGAG AAAGGGACTC AGCAATTATG CTGTAACATT TCAAAGAGGA 
GATGGAGGAC TAATGAGCAA TGGGAGCCGT CCAGGTCTTC TAAATGCTGG CGATCCCAAT 
TACCCATGGC TTGCTGATTC TTGGCCAGCC ACGAGTTTGC CAGTGAACAA TAGCAATAGT 
GGCCCAAATG AAATTGGAAA TTTTGGGCGT GGAGATGTGC TGCCTCCGGT GCCAGGCCAA 
GGGGATAAAA CAGCGACCAT GCTCTCGGAT GGAGCCATTT ATAGCAGCAT TGACTTCACT 
ACCAAAACCA CTTACAACAG TTCCAGCCAA ATAACACAGG CCACCCCATA TGCCACTACA 
CAAATCCTGC ATTCAAACAG CATCCACGAA CTGGCAGTTG ATCTTCCTGA TCCACAGTGG 
AAAAGCTCAG TTCAACAGAA GACAGACCTC ATGGGATTTG GTTATTCGCT ACCTGATCAG 
AACAAGGGGA ACAACGCCTT ACTTTACATC CCTGACTACC GATTGGCTGA GGGATTGTCT 
AATAGAATGC CACACAACCA GTCACAGGAT TTCAGCACCA CCAGCTCTCA CAACAGCTCA 
GAAAGGAGTG GCAGTCTCTC AGGTGGGAAA GGTGGAAAAA AGAAGAAAAC TAAAAATTCT 
TCGAAAGCGC AGAAAAACAA CGGATCCACT TGGGCTAATG TCCCTCTACC TCCTCCTCCC 
GTCCAGCCCC TTCCTGGTAC AGAGCTGGGC CACTATGCTG CGGAACAAGA AAATGGCTAT 
GACAGTGACA GCTGGTGCCC ACCATTACCG GTGCAAACAT ACTTGCATCA GGGTATGGAA 
GATGAGCTGG AAGAAGACGA AGATCGGGTC CCAACACCTC CTGTTCGCGG CGTGGCCTCT 
TCACCAGCTA TCTCTTTTGG ACAGCAGTCC ACTGCCACTC TTACTCCATC CCCACGGGAA 
GAGATGCAAC CCATGCTGCA AGCTCACTTG GATGAGTTGA CAAGGGCCTA TCAGTTTGAT 
ATAGCAAAAC AAACATGGCA CATTCAAAGC AATACCCCAC CTCCACAACC CCCAGCTCCG 
CCATTAGGTT ACGTGTCCGG AGCCTTGATT TCTGATTTGG AGACAGATGT TCCAGATGAG 
GATGCTGATG ATGAAGAGGA ACCATTAGAA ATTCCCAGGC CCCTCAGAGC ACTAGACCAG 
ACACCTGGAT CCAGTATGGA CAATCTAGAC AGCTCTGTCA CAGGAAAAGC CTTTAGCTCC 
TCTCAAAGGC AGCGGCCCAC CAGCCCATTT TCTACGGACA GTAACACCAG TGCTGCCCAG 
AATCAAAGCC AGAGGCCTCG GCCCACGAAA AAACATAAGG GAGGACGGAT GGACCCACAG 
CCAGTGTTAC CTCATCGGAG GGAAGGGATG CCAGATGATC TTCCCCCACC ACCAGATCCC 
CCACCAGGTC AGGGTTTAAG GCAGCAGATA GGCCTGAGCC AACACTCTGG TAACGTGGAA 
AACTCAACAG AGAGAAAAGG AAGCTCTCTA GAGAGACAAC AAGCAGCCAA CTTAGAAGAC 
ACAAAGAGCT CATTGGATTG TCCAGCTAAA ACTGTTCTAG AGTGGCAGCG TCAAACCCAG 
GACTGGATAA ACTCTACAGA ACGCCAAGAA GAAACTCGGA AAGCCCCACA CAAACAAGGG 
GTTGGATCAG AGGAGTCCCT GGTGCCCTAC AGCAAGCCCA GCTTCCCGTC TCCTGGCGGG 
CACAGCTCAT CAGGAACATC TTCCTCTAAA GGGTCCACTG GCCCTCGGAA GGCTGACGTG 
CTGAGAGGCA GCCACCAGAG GAATGCCAAT GACCTTCTCG ACATTGGATA TGTGGGCTCA 
AATAGTCAAG GACAGTTTAC AGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo2 ( NM_001358490.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001345419.1 Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant 4, mRNA.

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CloneID	OMu52764
Clone ID Related Accession (Same CDS sequence)	XM_006523044.3 , NM_001358490.1
Accession Version	NM_001358490.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4584bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	roundabout homolog 2 isoform 4 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC129186.3, AC158234.2, AC112155.3, AC134452.4, AC122049.2 and AC162374.4. On Dec 1, 2017 this sequence version replaced XM_006523044.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ533875.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END##

ATGGCCAGAA GACAAGAAAG TATCTGTGGA AGGCCGTGGA CGTGGACGCC AGGACTACTG 
ATGCTGATCA TCCTTGGCAT TCATCAGGGG AGTGGGCAAG GTCAAGGATC TCGTCTTCGT 
CAAGAAGACT TTCCCCCCAG GATCGTAGAA CACCCTTCTG ATGTCATCGT CTCCAAGGGA 
GAGCCCACCA CTCTGAACTG TAAAGCAGAG GGCCGACCCA CCCCCACCAT TGAATGGTAC 
AAGGATGGTG AGAGGGTGGA GACAGACAAG GATGATCCCA GGTCCCACAG AATGCTTCTG 
CCCAGCGGAT CTTTATTCTT TTTGCGAATT GTTCATGGGC GCAGAAGTAA ACCGGACGAA 
GGGAGTTACG TTTGTGTTGC AAGGAACTAT CTTGGTGAAG CAGTGAGTCG AAATGCATCT 
CTGGAAGTGG CATTATTGCG AGATGACTTC CGGCAAAACC CCACAGATGT GGTAGTCGCA 
GCTGGAGAGC CTGCAATCTT GGAGTGCCAG CCACCACGGG GACACCCAGA ACCAACCATC 
TACTGGAAAA AGGACAAAGT CCGAATTGAT GACAAGGAAG AGAGAATAAG TATCCGTGGT 
GGGAAGCTGA TGATCTCTAA TACTAGGAAA AGCGATGCTG GCATGTACAC CTGTGTGGGA 
ACCAATATGG TGGGAGAAAG GGACAGCGAC CCTGCAGAGC TCACTGTCTT TGAACGACCC 
ACATTTCTCA GGAGGCCAAT TAACCAGGTG GTGCTAGAGG AAGAAGCTGT AGAATTCCGT 
TGTCAGGTCC AAGGAGATCC CCAGCCAACG GTGAGGTGGA AAAAAGATGA TGCAGACTTG 
CCGAGAGGAA GGTATGATAT CAAAGATGAC TACACGCTGA GAATTAAAAA GGCCATGAGT 
ACTGATGAAG GTACCTATGT GTGTATTGCT GAGAATCGGG TGGGAAAAGT GGAAGCCTCT 
GCTACCCTCA CTGTCCGAGT TCGCCCTGTT GCTCCTCCAC AGTTTGTGGT TAGGCCAAGA 
GATCAGATCG TTGCTCAAGG CCGAACAGTG ACATTCCCCT GTGAAACTAA AGGAAACCCA 
CAGCCAGCTG TTTTTTGGCA GAAAGAAGGC AGCCAGAACC TACTTTTCCC GAATCAACCT 
CAGCAGCCCA ACAGCCGATG TTCAGTGTCG CCCACGGGGG ACCTCACCAT CACCAACATC 
CAGCGTTCAG ATGCGGGTTA CTACATCTGC CAGGCCCTAA CCGTGGCAGG AAGCATTTTA 
GCTAAAGCAC AGTTGGAAGT TACTGACGTT TTGACAGATA GACCTCCACC CATAATCTTG 
CAAGGACCAA TAAACCAAAC ACTTGCAGTA GACGGTACAG CATTGTTGAA GTGTAAAGCC 
ACTGGTGAGC CTCTGCCTGT AATTAGCTGG CTAAAGGAGG GCTTTACTTT TCTGGGGAGA 
GATCCAAGAG CCACGATCCA AGACCAAGGA ACACTGCAGA TTAAGAATTT ACGGATATCT 
GATACTGGCA CTTATACTTG TGTGGCTACA AGTTCCAGTG GAGAGACTTC CTGGAGTGCA 
GTGCTGGATG TAACAGAATC TGGAGCAACA ATCAGTAAAA ATTATGATAT GAATGACCTC 
CCGGGACCAC CATCCAAACC TCAGGTCACT GATGTTTCTA AGAACAGTGT CACCTTATCC 
TGGCAGCCAG GTACACCTGG CGTTCTTCCT GCAAGCGCGT ATATCATTGA GGCTTTCAGC 
CAATCGGTGA GCAATAGCTG GCAGACAGTG GCAAACCATG TTAAGACAAC TCTGTATACA 
GTAAGGGGGC TGAGGCCCAA CACAATCTAC TTGTTTATGG TCAGAGCGAT CAACCCACAA 
GGTCTCAGTG ATCCAAGTCC TATGTCGGAT CCTGTACGCA CACAAGATAT CAGCCCCCCA 
GCACAAGGAG TGGACCACAG ACAGGTGCAG AAGGAATTAG GTGATGTCGT TGTTCGTCTC 
CATAATCCAG TTGTCCTGAC ACCTACAACT GTTCAAGTCA CATGGACGGT GGACCGACAA 
CCCCAGTTTA TTCAGGGCTA CAGAGTGATG TACCGTCAGA CTTCGGGACT ACAAGCCTCA 
ACTGTGTGGC AGAATCTAGA CGCCAAAGTC CCGACTGAGA GGAGTGCTGT CCTTGTGAAT 
TTGAAAAAGG GGGTGACTTA TGAAATTAAA GTCCGGCCGT ATTTTAACGA GTTCCAAGGA 
ATGGACAGTG AATCGAAAAC AGTCCGAACC ACTGAGGAAG CCCCAAGTGC CCCTCCCCAG 
TCTGTCACTG TGCTGACAGT TGGAAGTCAC AACAGCACAA GCATCAGTGT TTCCTGGGAT 
CCTCCACCAG CCGACCACCA GAATGGAATT ATTCAGGAAT ATAAGATCTG GTGTCTGGGA 
AACGAAACGC GATTCCATAT CAATAAAACG GTGGATGCAG CCATTCGCTC TGTAGTAATA 
GGTGGCTTGT TCCCTGGAAT TCAGTACCGG GTAGAAGTGG CAGCTAGCAC AAGTGCAGGG 
GTTGGAGTAA AAAGTGAACC ACAGCCGATA ATAATTGGGG GACGTAACGA AGTTGTCATT 
ACTGAAAACA ATAACAGCAT CACTGAGCAA ATCACGGATG TCGTGAAGCA ACCGGCATTT 
ATAGCTGGCA TTGGTGGTGC CTGCTGGGTA ATTCTGATGG GTTTTAGCAT CTGGTTGTAC 
TGGAGAAGAA AGAAGAGAAA GGGACTCAGC AATTATGCTG TAACATTTCA AAGAGGAGAT 
GGAGGACTAA TGAGCAATGG GAGCCGTCCA GGTCTTCTAA ATGCTGGCGA TCCCAATTAC 
CCATGGCTTG CTGATTCTTG GCCAGCCACG AGTTTGCCAG TGAACAATAG CAATAGTGGC 
CCAAATGAAA TTGGAAATTT TGGGCGTGGA GATGTGCTGC CTCCGGTGCC AGGCCAAGGG 
GATAAAACAG CGACCATGCT CTCGGATGGA GCCATTTATA GCAGCATTGA CTTCACTACC 
AAAACCACTT ACAACAGTTC CAGCCAAATA ACACAGGCCA CCCCATATGC CACTACACAA 
ATCCTGCATT CAAACAGCAT CCACGAACTG GCAGTTGATC TTCCTGATCC ACAGTGGAAA 
AGCTCAGTTC AACAGAAGAC AGACCTCATG GGATTTGGTT ATTCGCTACC TGATCAGAAC 
AAGGGGAACA ACGCCTTACT TTACATCCCT GACTACCGAT TGGCTGAGGG ATTGTCTAAT 
AGAATGCCAC ACAACCAGTC ACAGGATTTC AGCACCACCA GCTCTCACAA CAGCTCAGAA 
AGGAGTGGCA GTCTCTCAGG TGGGAAAGGT GGAAAAAAGA AGAAAACTAA AAATTCTTCG 
AAAGCGCAGA AAAACAACGG ATCCACTTGG GCTAATGTCC CTCTACCTCC TCCTCCCGTC 
CAGCCCCTTC CTGGTACAGA GCTGGGCCAC TATGCTGCGG AACAAGAAAA TGGCTATGAC 
AGTGACAGCT GGTGCCCACC ATTACCGGTG CAAACATACT TGCATCAGGG TATGGAAGAT 
GAGCTGGAAG AAGACGAAGA TCGGGTCCCA ACACCTCCTG TTCGCGGCGT GGCCTCTTCA 
CCAGCTATCT CTTTTGGACA GCAGTCCACT GCCACTCTTA CTCCATCCCC ACGGGAAGAG 
ATGCAACCCA TGCTGCAAGC TCACTTGGAT GAGTTGACAA GGGCCTATCA GTTTGATATA 
GCAAAACAAA CATGGCACAT TCAAAGCAAT ACCCCACCTC CACAACCCCC AGCTCCGCCA 
TTAGGTTACG TGTCCGGAGC CTTGATTTCT GATTTGGAGA CAGATGTTCC AGATGAGGAT 
GCTGATGATG AAGAGGAACC ATTAGAAATT CCCAGGCCCC TCAGAGCACT AGACCAGACA 
CCTGGATCCA GTATGGACAA TCTAGACAGC TCTGTCACAG GAAAAGCCTT TAGCTCCTCT 
CAAAGGCAGC GGCCCACCAG CCCATTTTCT ACGGACAGTA ACACCAGTGC TGCCCAGAAT 
CAAAGCCAGA GGCCTCGGCC CACGAAAAAA CATAAGGGAG GACGGATGGA CCCACAGCCA 
GTGTTACCTC ATCGGAGGGA AGGGATGCCA GATGATCTTC CCCCACCACC AGATCCCCCA 
CCAGGTCAGG GTTTAAGGCA GCAGATAGGC CTGAGCCAAC ACTCTGGTAA CGTGGAAAAC 
TCAACAGAGA GAAAAGGAAG CTCTCTAGAG AGACAACAAG CAGCCAACTT AGAAGACACA 
AAGAGCTCAT TGGATTGTCC AGCTAAAACT GTTCTAGAGT GGCAGCGTCA AACCCAGGAC 
TGGATAAACT CTACAGAACG CCAAGAAGAA ACTCGGAAAG CCCCACACAA ACAAGGGGTT 
GGATCAGAGG AGTCCCTGGT GCCCTACAGC AAGCCCAGCT TCCCGTCTCC TGGCGGGCAC 
AGCTCATCAG GAACATCTTC CTCTAAAGGG TCCACTGGCC CTCGGAAGGC TGACGTGCTG 
AGAGGCAGCC ACCAGAGGAA TGCCAATGAC CTTCTCGACA TTGGATATGT GGGCTCAAAT 
AGTCAAGGAC AGTTTACAGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001345419.1
CDS	236..4819
Translation	MARRQESICGRPWTWTPGLLMLIILGIHQGSGQGQGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKAMSTDEGTYVCIAENRVGKVEASATLTVRVRPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPINQTLAVDGTALLKCKATGEPLPVISWLKEGFTFLGRDPRATIQDQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDMNDLPGPPSKPQVTDVSKNSVTLSWQPGTPGVLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVVVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQASTVWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSHNSTSISVSWDPPPADHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVVIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGGRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPNYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTTYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSVQQKTDLMGFGYSLPDQNKGNNALLYIPDYRLAEGLSNRMPHNQSQDFSTTSSHNSSERSGSLSGGKGGKKKKTKNSSKAQKNNGSTWANVPLPPPPVQPLPGTELGHYAAEQENGYDSDSWCPPLPVQTYLHQGMEDELEEDEDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNTPPPQPPAPPLGYVSGALISDLETDVPDEDADDEEEPLEIPRPLRALDQTPGSSMDNLDSSVTGKAFSSSQRQRPTSPFSTDSNTSAAQNQSQRPRPTKKHKGGRMDPQPVLPHRREGMPDDLPPPPDPPPGQGLRQQIGLSQHSGNVENSTERKGSSLERQQAANLEDTKSSLDCPAKTVLEWQRQTQDWINSTERQEETRKAPHKQGVGSEESLVPYSKPSFPSPGGHSSSGTSSSKGSTGPRKADVLRGSHQRNANDLLDIGYVGSNSQGQFTE

Target ORF information:

RefSeq Version	NM_001358490.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001358490.1

ORF Insert Sequence:

ATGGCCAGAA GACAAGAAAG TATCTGTGGA AGGCCGTGGA CGTGGACGCC AGGACTACTG 
ATGCTGATCA TCCTTGGCAT TCATCAGGGG AGTGGGCAAG GTCAAGGATC TCGTCTTCGT 
CAAGAAGACT TTCCCCCCAG GATCGTAGAA CACCCTTCTG ATGTCATCGT CTCCAAGGGA 
GAGCCCACCA CTCTGAACTG TAAAGCAGAG GGCCGACCCA CCCCCACCAT TGAATGGTAC 
AAGGATGGTG AGAGGGTGGA GACAGACAAG GATGATCCCA GGTCCCACAG AATGCTTCTG 
CCCAGCGGAT CTTTATTCTT TTTGCGAATT GTTCATGGGC GCAGAAGTAA ACCGGACGAA 
GGGAGTTACG TTTGTGTTGC AAGGAACTAT CTTGGTGAAG CAGTGAGTCG AAATGCATCT 
CTGGAAGTGG CATTATTGCG AGATGACTTC CGGCAAAACC CCACAGATGT GGTAGTCGCA 
GCTGGAGAGC CTGCAATCTT GGAGTGCCAG CCACCACGGG GACACCCAGA ACCAACCATC 
TACTGGAAAA AGGACAAAGT CCGAATTGAT GACAAGGAAG AGAGAATAAG TATCCGTGGT 
GGGAAGCTGA TGATCTCTAA TACTAGGAAA AGCGATGCTG GCATGTACAC CTGTGTGGGA 
ACCAATATGG TGGGAGAAAG GGACAGCGAC CCTGCAGAGC TCACTGTCTT TGAACGACCC 
ACATTTCTCA GGAGGCCAAT TAACCAGGTG GTGCTAGAGG AAGAAGCTGT AGAATTCCGT 
TGTCAGGTCC AAGGAGATCC CCAGCCAACG GTGAGGTGGA AAAAAGATGA TGCAGACTTG 
CCGAGAGGAA GGTATGATAT CAAAGATGAC TACACGCTGA GAATTAAAAA GGCCATGAGT 
ACTGATGAAG GTACCTATGT GTGTATTGCT GAGAATCGGG TGGGAAAAGT GGAAGCCTCT 
GCTACCCTCA CTGTCCGAGT TCGCCCTGTT GCTCCTCCAC AGTTTGTGGT TAGGCCAAGA 
GATCAGATCG TTGCTCAAGG CCGAACAGTG ACATTCCCCT GTGAAACTAA AGGAAACCCA 
CAGCCAGCTG TTTTTTGGCA GAAAGAAGGC AGCCAGAACC TACTTTTCCC GAATCAACCT 
CAGCAGCCCA ACAGCCGATG TTCAGTGTCG CCCACGGGGG ACCTCACCAT CACCAACATC 
CAGCGTTCAG ATGCGGGTTA CTACATCTGC CAGGCCCTAA CCGTGGCAGG AAGCATTTTA 
GCTAAAGCAC AGTTGGAAGT TACTGACGTT TTGACAGATA GACCTCCACC CATAATCTTG 
CAAGGACCAA TAAACCAAAC ACTTGCAGTA GACGGTACAG CATTGTTGAA GTGTAAAGCC 
ACTGGTGAGC CTCTGCCTGT AATTAGCTGG CTAAAGGAGG GCTTTACTTT TCTGGGGAGA 
GATCCAAGAG CCACGATCCA AGACCAAGGA ACACTGCAGA TTAAGAATTT ACGGATATCT 
GATACTGGCA CTTATACTTG TGTGGCTACA AGTTCCAGTG GAGAGACTTC CTGGAGTGCA 
GTGCTGGATG TAACAGAATC TGGAGCAACA ATCAGTAAAA ATTATGATAT GAATGACCTC 
CCGGGACCAC CATCCAAACC TCAGGTCACT GATGTTTCTA AGAACAGTGT CACCTTATCC 
TGGCAGCCAG GTACACCTGG CGTTCTTCCT GCAAGCGCGT ATATCATTGA GGCTTTCAGC 
CAATCGGTGA GCAATAGCTG GCAGACAGTG GCAAACCATG TTAAGACAAC TCTGTATACA 
GTAAGGGGGC TGAGGCCCAA CACAATCTAC TTGTTTATGG TCAGAGCGAT CAACCCACAA 
GGTCTCAGTG ATCCAAGTCC TATGTCGGAT CCTGTACGCA CACAAGATAT CAGCCCCCCA 
GCACAAGGAG TGGACCACAG ACAGGTGCAG AAGGAATTAG GTGATGTCGT TGTTCGTCTC 
CATAATCCAG TTGTCCTGAC ACCTACAACT GTTCAAGTCA CATGGACGGT GGACCGACAA 
CCCCAGTTTA TTCAGGGCTA CAGAGTGATG TACCGTCAGA CTTCGGGACT ACAAGCCTCA 
ACTGTGTGGC AGAATCTAGA CGCCAAAGTC CCGACTGAGA GGAGTGCTGT CCTTGTGAAT 
TTGAAAAAGG GGGTGACTTA TGAAATTAAA GTCCGGCCGT ATTTTAACGA GTTCCAAGGA 
ATGGACAGTG AATCGAAAAC AGTCCGAACC ACTGAGGAAG CCCCAAGTGC CCCTCCCCAG 
TCTGTCACTG TGCTGACAGT TGGAAGTCAC AACAGCACAA GCATCAGTGT TTCCTGGGAT 
CCTCCACCAG CCGACCACCA GAATGGAATT ATTCAGGAAT ATAAGATCTG GTGTCTGGGA 
AACGAAACGC GATTCCATAT CAATAAAACG GTGGATGCAG CCATTCGCTC TGTAGTAATA 
GGTGGCTTGT TCCCTGGAAT TCAGTACCGG GTAGAAGTGG CAGCTAGCAC AAGTGCAGGG 
GTTGGAGTAA AAAGTGAACC ACAGCCGATA ATAATTGGGG GACGTAACGA AGTTGTCATT 
ACTGAAAACA ATAACAGCAT CACTGAGCAA ATCACGGATG TCGTGAAGCA ACCGGCATTT 
ATAGCTGGCA TTGGTGGTGC CTGCTGGGTA ATTCTGATGG GTTTTAGCAT CTGGTTGTAC 
TGGAGAAGAA AGAAGAGAAA GGGACTCAGC AATTATGCTG TAACATTTCA AAGAGGAGAT 
GGAGGACTAA TGAGCAATGG GAGCCGTCCA GGTCTTCTAA ATGCTGGCGA TCCCAATTAC 
CCATGGCTTG CTGATTCTTG GCCAGCCACG AGTTTGCCAG TGAACAATAG CAATAGTGGC 
CCAAATGAAA TTGGAAATTT TGGGCGTGGA GATGTGCTGC CTCCGGTGCC AGGCCAAGGG 
GATAAAACAG CGACCATGCT CTCGGATGGA GCCATTTATA GCAGCATTGA CTTCACTACC 
AAAACCACTT ACAACAGTTC CAGCCAAATA ACACAGGCCA CCCCATATGC CACTACACAA 
ATCCTGCATT CAAACAGCAT CCACGAACTG GCAGTTGATC TTCCTGATCC ACAGTGGAAA 
AGCTCAGTTC AACAGAAGAC AGACCTCATG GGATTTGGTT ATTCGCTACC TGATCAGAAC 
AAGGGGAACA ACGCCTTACT TTACATCCCT GACTACCGAT TGGCTGAGGG ATTGTCTAAT 
AGAATGCCAC ACAACCAGTC ACAGGATTTC AGCACCACCA GCTCTCACAA CAGCTCAGAA 
AGGAGTGGCA GTCTCTCAGG TGGGAAAGGT GGAAAAAAGA AGAAAACTAA AAATTCTTCG 
AAAGCGCAGA AAAACAACGG ATCCACTTGG GCTAATGTCC CTCTACCTCC TCCTCCCGTC 
CAGCCCCTTC CTGGTACAGA GCTGGGCCAC TATGCTGCGG AACAAGAAAA TGGCTATGAC 
AGTGACAGCT GGTGCCCACC ATTACCGGTG CAAACATACT TGCATCAGGG TATGGAAGAT 
GAGCTGGAAG AAGACGAAGA TCGGGTCCCA ACACCTCCTG TTCGCGGCGT GGCCTCTTCA 
CCAGCTATCT CTTTTGGACA GCAGTCCACT GCCACTCTTA CTCCATCCCC ACGGGAAGAG 
ATGCAACCCA TGCTGCAAGC TCACTTGGAT GAGTTGACAA GGGCCTATCA GTTTGATATA 
GCAAAACAAA CATGGCACAT TCAAAGCAAT ACCCCACCTC CACAACCCCC AGCTCCGCCA 
TTAGGTTACG TGTCCGGAGC CTTGATTTCT GATTTGGAGA CAGATGTTCC AGATGAGGAT 
GCTGATGATG AAGAGGAACC ATTAGAAATT CCCAGGCCCC TCAGAGCACT AGACCAGACA 
CCTGGATCCA GTATGGACAA TCTAGACAGC TCTGTCACAG GAAAAGCCTT TAGCTCCTCT 
CAAAGGCAGC GGCCCACCAG CCCATTTTCT ACGGACAGTA ACACCAGTGC TGCCCAGAAT 
CAAAGCCAGA GGCCTCGGCC CACGAAAAAA CATAAGGGAG GACGGATGGA CCCACAGCCA 
GTGTTACCTC ATCGGAGGGA AGGGATGCCA GATGATCTTC CCCCACCACC AGATCCCCCA 
CCAGGTCAGG GTTTAAGGCA GCAGATAGGC CTGAGCCAAC ACTCTGGTAA CGTGGAAAAC 
TCAACAGAGA GAAAAGGAAG CTCTCTAGAG AGACAACAAG CAGCCAACTT AGAAGACACA 
AAGAGCTCAT TGGATTGTCC AGCTAAAACT GTTCTAGAGT GGCAGCGTCA AACCCAGGAC 
TGGATAAACT CTACAGAACG CCAAGAAGAA ACTCGGAAAG CCCCACACAA ACAAGGGGTT 
GGATCAGAGG AGTCCCTGGT GCCCTACAGC AAGCCCAGCT TCCCGTCTCC TGGCGGGCAC 
AGCTCATCAG GAACATCTTC CTCTAAAGGG TCCACTGGCC CTCGGAAGGC TGACGTGCTG 
AGAGGCAGCC ACCAGAGGAA TGCCAATGAC CTTCTCGACA TTGGATATGT GGGCTCAAAT 
AGTCAAGGAC AGTTTACAGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo2 ( NM_001358491.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001345420.1 Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu52767
Clone ID Related Accession (Same CDS sequence)	NM_001358491.1 , XM_006523047.3
Accession Version	NM_001358491.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4482bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-22
Organism	Mus musculus(house mouse)
Product	roundabout homolog 2 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC115120.4, AC158234.2, AC112155.3, AC134452.4, AC122049.2 and AC162374.4. On Dec 2, 2017 this sequence version replaced XM_006523047.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660815.22491.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END##

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT 
CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG 
GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC 
CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA 
GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT 
ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG 
GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG 
CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT 
AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT 
TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC 
GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC 
CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT 
ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA 
CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA 
TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA 
GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT 
CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT 
GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG 
CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG 
GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC 
ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG 
GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT 
GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA 
GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT 
GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG 
GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT 
GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT 
CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG 
CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG 
CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT 
TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG 
GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT 
GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT 
GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGGTG GGAAAGGTGG AAAAAAGAAG 
AAAACTAAAA ATTCTTCGAA AGCGCAGAAA AACAACGGAT CCACTTGGGC TAATGTCCCT 
CTACCTCCTC CTCCCGTCCA GCCCCTTCCT GGTACAGAGC TGGGCCACTA TGCTGCGGAA 
CAAGAAAATG GCTATGACAG TGACAGCTGG TGCCCACCAT TACCGGTGCA AACATACTTG 
CATCAGGGTA TGGAAGATGA GCTGGAAGAA GACGAAGATC GGGTCCCAAC ACCTCCTGTT 
CGCGGCGTGG CCTCTTCACC AGCTATCTCT TTTGGACAGC AGTCCACTGC CACTCTTACT 
CCATCCCCAC GGGAAGAGAT GCAACCCATG CTGCAAGCTC ACTTGGATGA GTTGACAAGG 
GCCTATCAGT TTGATATAGC AAAACAAACA TGGCACATTC AAAGCAATAC CCCACCTCCA 
CAACCCCCAG CTCCGCCATT AGGTTACGTG TCCGGAGCCT TGATTTCTGA TTTGGAGACA 
GATGTTCCAG ATGAGGATGC TGATGATGAA GAGGAACCAT TAGAAATTCC CAGGCCCCTC 
AGAGCACTAG ACCAGACACC TGGATCCAGT ATGGACAATC TAGACAGCTC TGTCACAGGA 
AAAGCCTTTA GCTCCTCTCA AAGGCAGCGG CCCACCAGCC CATTTTCTAC GGACAGTAAC 
ACCAGTGCTG CCCAGAATCA AAGCCAGAGG CCTCGGCCCA CGAAAAAACA TAAGGGAGGA 
CGGATGGACC CACAGCCAGT GTTACCTCAT CGGAGGGAAG GGATGCCAGA TGATCTTCCC 
CCACCACCAG ATCCCCCACC AGGTCAGGGT TTAAGGCAGC AGATAGGCCT GAGCCAACAC 
TCTGGTAACG TGGAAAACTC AACAGAGAGA AAAGGAAGCT CTCTAGAGAG ACAACAAGCA 
GCCAACTTAG AAGACACAAA GAGCTCATTG GATTGTCCAG CTAAAACTGT TCTAGAGTGG 
CAGCGTCAAA CCCAGGACTG GATAAACTCT ACAGAACGCC AAGAAGAAAC TCGGAAAGCC 
CCACACAAAC AAGGGGTTGG ATCAGAGGAG TCCCTGGTGC CCTACAGCAA GCCCAGCTTC 
CCGTCTCCTG GCGGGCACAG CTCATCAGGA ACATCTTCCT CTAAAGGGTC CACTGGCCCT 
CGGAAGGCTG ACGTGCTGAG AGGCAGCCAC CAGAGGAATG CCAATGACCT TCTCGACATT 
GGATATGTGG GCTCAAATAG TCAAGGACAG TTTACAGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001345420.1
CDS	353..4834
Translation	MLSSEKVDKITRLELLFVLMLFFSGPTLGKLTRTPWKGKPWKGGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKAMSTDEGTYVCIAENRVGKVEASATLTVRVRPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPINQTLAVDGTALLKCKATGEPLPVISWLKEGFTFLGRDPRATIQDQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDMNDLPGPPSKPQVTDVSKNSVTLSWQPGTPGVLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVVVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQASTVWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSHNSTSISVSWDPPPADHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVVIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGGRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPNYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTTYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSVQQKTDLMGFGYSLPDQNKGNNGGKGGKKKKTKNSSKAQKNNGSTWANVPLPPPPVQPLPGTELGHYAAEQENGYDSDSWCPPLPVQTYLHQGMEDELEEDEDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNTPPPQPPAPPLGYVSGALISDLETDVPDEDADDEEEPLEIPRPLRALDQTPGSSMDNLDSSVTGKAFSSSQRQRPTSPFSTDSNTSAAQNQSQRPRPTKKHKGGRMDPQPVLPHRREGMPDDLPPPPDPPPGQGLRQQIGLSQHSGNVENSTERKGSSLERQQAANLEDTKSSLDCPAKTVLEWQRQTQDWINSTERQEETRKAPHKQGVGSEESLVPYSKPSFPSPGGHSSSGTSSSKGSTGPRKADVLRGSHQRNANDLLDIGYVGSNSQGQFTE

Target ORF information:

RefSeq Version	NM_001358491.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001358491.1

ORF Insert Sequence:

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT 
CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG 
GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC 
CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA 
GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT 
ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG 
GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG 
CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT 
AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT 
TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC 
GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC 
CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT 
ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA 
CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA 
TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA 
GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT 
CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT 
GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG 
CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG 
GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC 
ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG 
GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT 
GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA 
GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT 
GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG 
GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT 
GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT 
CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG 
CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG 
CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT 
TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG 
GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT 
GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT 
GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGGTG GGAAAGGTGG AAAAAAGAAG 
AAAACTAAAA ATTCTTCGAA AGCGCAGAAA AACAACGGAT CCACTTGGGC TAATGTCCCT 
CTACCTCCTC CTCCCGTCCA GCCCCTTCCT GGTACAGAGC TGGGCCACTA TGCTGCGGAA 
CAAGAAAATG GCTATGACAG TGACAGCTGG TGCCCACCAT TACCGGTGCA AACATACTTG 
CATCAGGGTA TGGAAGATGA GCTGGAAGAA GACGAAGATC GGGTCCCAAC ACCTCCTGTT 
CGCGGCGTGG CCTCTTCACC AGCTATCTCT TTTGGACAGC AGTCCACTGC CACTCTTACT 
CCATCCCCAC GGGAAGAGAT GCAACCCATG CTGCAAGCTC ACTTGGATGA GTTGACAAGG 
GCCTATCAGT TTGATATAGC AAAACAAACA TGGCACATTC AAAGCAATAC CCCACCTCCA 
CAACCCCCAG CTCCGCCATT AGGTTACGTG TCCGGAGCCT TGATTTCTGA TTTGGAGACA 
GATGTTCCAG ATGAGGATGC TGATGATGAA GAGGAACCAT TAGAAATTCC CAGGCCCCTC 
AGAGCACTAG ACCAGACACC TGGATCCAGT ATGGACAATC TAGACAGCTC TGTCACAGGA 
AAAGCCTTTA GCTCCTCTCA AAGGCAGCGG CCCACCAGCC CATTTTCTAC GGACAGTAAC 
ACCAGTGCTG CCCAGAATCA AAGCCAGAGG CCTCGGCCCA CGAAAAAACA TAAGGGAGGA 
CGGATGGACC CACAGCCAGT GTTACCTCAT CGGAGGGAAG GGATGCCAGA TGATCTTCCC 
CCACCACCAG ATCCCCCACC AGGTCAGGGT TTAAGGCAGC AGATAGGCCT GAGCCAACAC 
TCTGGTAACG TGGAAAACTC AACAGAGAGA AAAGGAAGCT CTCTAGAGAG ACAACAAGCA 
GCCAACTTAG AAGACACAAA GAGCTCATTG GATTGTCCAG CTAAAACTGT TCTAGAGTGG 
CAGCGTCAAA CCCAGGACTG GATAAACTCT ACAGAACGCC AAGAAGAAAC TCGGAAAGCC 
CCACACAAAC AAGGGGTTGG ATCAGAGGAG TCCCTGGTGC CCTACAGCAA GCCCAGCTTC 
CCGTCTCCTG GCGGGCACAG CTCATCAGGA ACATCTTCCT CTAAAGGGTC CACTGGCCCT 
CGGAAGGCTG ACGTGCTGAG AGGCAGCCAC CAGAGGAATG CCAATGACCT TCTCGACATT 
GGATATGTGG GCTCAAATAG TCAAGGACAG TTTACAGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo2 ( NM_001358492.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001345421.1 Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu74356
Clone ID Related Accession (Same CDS sequence)	NM_001358492.1 , XM_011246132.2
Accession Version	NM_001358492.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4215bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-22
Organism	Mus musculus(house mouse)
Product	roundabout homolog 2 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC115120.4, AC158234.2, AC112155.3, AC134452.4, AC122049.2 and AC162374.4. On Dec 1, 2017 this sequence version replaced XM_011246132.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660819.50109.1, SRR1660811.204850.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END##

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT 
CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG 
GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC 
CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA 
GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT 
ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG 
GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG 
CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT 
AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT 
TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC 
GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC 
CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT 
ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA 
CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA 
TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA 
GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT 
CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT 
GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG 
CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG 
GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC 
ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG 
GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT 
GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA 
GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT 
GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG 
GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT 
GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT 
CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG 
CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG 
CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT 
TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG 
GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT 
GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT 
GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGGTG GGAAAGGTGG AAAAAAGAAG 
AAAACTAAAA ATTCTTCGAA AGCGCAGAAA AACAACGGAT CCACTTGGGC TAATGTCCCT 
CTACCTCCTC CTCCCGTCCA GCCCCTTCCT GGTACAGAGC TGGGCCACTA TGCTGCGGAA 
CAAGAAAATG GCTATGACAG TGACAGCTGG TGCCCACCAT TACCGGTGCA AACATACTTG 
CATCAGGGTA TGGAAGATGA GCTGGAAGAA GACGAAGATC GGGTCCCAAC ACCTCCTGTT 
CGCGGCGTGG CCTCTTCACC AGCTATCTCT TTTGGACAGC AGTCCACTGC CACTCTTACT 
CCATCCCCAC GGGAAGAGAT GCAACCCATG CTGCAAGCTC ACTTGGATGA GTTGACAAGG 
GCCTATCAGT TTGATATAGC AAAACAAACA TGGCACATTC AAAGCAATAC CCCACCTCCA 
CAACCCCCAG CTCCGCCATT AGGTTACGTG TCCGGAGCCT TGATTTCTGA TTTGGAGACA 
GATGTTCCAG ATGAGGATGC TGATGATGAA GAGGAACCAT TAGAAATTCC CAGGCCCCTC 
AGAGCACTAG ACCAGACACC TGGATCCAGT ATGGACAATC TAGACAGCTC TGTCACAGGA 
AAAGCCTTTA GCTCCTCTCA AAGGCAGCGG CCCACCAGCC CATTTTCTAC GGACAGTAAC 
ACCAGTGCTG CCCAGAATCA AAGCCAGAGG CCTCGGCCCA CGAAAAAACA TAAGGGAGGA 
CGGATGGACC CACAGCCAGT GTTACCTCAT CGGAGGGAAG GGATGCCAGA TGAGGAGTCC 
CTGGTGCCCT ACAGCAAGCC CAGCTTCCCG TCTCCTGGCG GGCACAGCTC ATCAGGAACA 
TCTTCCTCTA AAGGGTCCAC TGGCCCTCGG AAGGCTGACG TGCTGAGAGG CAGCCACCAG 
AGGAATGCCA ATGACCTTCT CGACATTGGA TATGTGGGCT CAAATAGTCA AGGACAGTTT 
ACAGGTGAAT TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001345421.1
CDS	353..4567
Translation	MLSSEKVDKITRLELLFVLMLFFSGPTLGKLTRTPWKGKPWKGGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKAMSTDEGTYVCIAENRVGKVEASATLTVRVRPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPINQTLAVDGTALLKCKATGEPLPVISWLKEGFTFLGRDPRATIQDQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDMNDLPGPPSKPQVTDVSKNSVTLSWQPGTPGVLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVVVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQASTVWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSHNSTSISVSWDPPPADHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVVIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGGRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPNYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTTYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSVQQKTDLMGFGYSLPDQNKGNNGGKGGKKKKTKNSSKAQKNNGSTWANVPLPPPPVQPLPGTELGHYAAEQENGYDSDSWCPPLPVQTYLHQGMEDELEEDEDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNTPPPQPPAPPLGYVSGALISDLETDVPDEDADDEEEPLEIPRPLRALDQTPGSSMDNLDSSVTGKAFSSSQRQRPTSPFSTDSNTSAAQNQSQRPRPTKKHKGGRMDPQPVLPHRREGMPDEESLVPYSKPSFPSPGGHSSSGTSSSKGSTGPRKADVLRGSHQRNANDLLDIGYVGSNSQGQFTGEL

Target ORF information:

RefSeq Version	NM_001358492.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001358492.1

ORF Insert Sequence:

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT 
CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG 
GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC 
CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA 
GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT 
ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG 
GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG 
CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT 
AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT 
TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC 
GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC 
CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT 
ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA 
CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA 
TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA 
GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT 
CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT 
GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG 
CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG 
GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC 
ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG 
GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT 
GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA 
GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT 
GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG 
GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT 
GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT 
CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG 
CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG 
CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT 
TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG 
GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT 
GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT 
GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGGTG GGAAAGGTGG AAAAAAGAAG 
AAAACTAAAA ATTCTTCGAA AGCGCAGAAA AACAACGGAT CCACTTGGGC TAATGTCCCT 
CTACCTCCTC CTCCCGTCCA GCCCCTTCCT GGTACAGAGC TGGGCCACTA TGCTGCGGAA 
CAAGAAAATG GCTATGACAG TGACAGCTGG TGCCCACCAT TACCGGTGCA AACATACTTG 
CATCAGGGTA TGGAAGATGA GCTGGAAGAA GACGAAGATC GGGTCCCAAC ACCTCCTGTT 
CGCGGCGTGG CCTCTTCACC AGCTATCTCT TTTGGACAGC AGTCCACTGC CACTCTTACT 
CCATCCCCAC GGGAAGAGAT GCAACCCATG CTGCAAGCTC ACTTGGATGA GTTGACAAGG 
GCCTATCAGT TTGATATAGC AAAACAAACA TGGCACATTC AAAGCAATAC CCCACCTCCA 
CAACCCCCAG CTCCGCCATT AGGTTACGTG TCCGGAGCCT TGATTTCTGA TTTGGAGACA 
GATGTTCCAG ATGAGGATGC TGATGATGAA GAGGAACCAT TAGAAATTCC CAGGCCCCTC 
AGAGCACTAG ACCAGACACC TGGATCCAGT ATGGACAATC TAGACAGCTC TGTCACAGGA 
AAAGCCTTTA GCTCCTCTCA AAGGCAGCGG CCCACCAGCC CATTTTCTAC GGACAGTAAC 
ACCAGTGCTG CCCAGAATCA AAGCCAGAGG CCTCGGCCCA CGAAAAAACA TAAGGGAGGA 
CGGATGGACC CACAGCCAGT GTTACCTCAT CGGAGGGAAG GGATGCCAGA TGAGGAGTCC 
CTGGTGCCCT ACAGCAAGCC CAGCTTCCCG TCTCCTGGCG GGCACAGCTC ATCAGGAACA 
TCTTCCTCTA AAGGGTCCAC TGGCCCTCGG AAGGCTGACG TGCTGAGAGG CAGCCACCAG 
AGGAATGCCA ATGACCTTCT CGACATTGGA TATGTGGGCT CAAATAGTCA AGGACAGTTT 
ACAGGTGAAT TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo2 ( XM_006523035.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523098.1 Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X1, mRNA.

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CloneID	OMu52755
Clone ID Related Accession (Same CDS sequence)	XM_006523035.4 , XM_006523035.3
Accession Version	XM_006523035.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4797bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	roundabout homolog 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006523035.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT 
CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG 
GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC 
CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA 
GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT 
ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG 
GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG 
CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT 
AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT 
TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC 
GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC 
CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT 
ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA 
CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA 
TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA 
GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT 
CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT 
GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG 
CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG 
GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC 
ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG 
GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT 
GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA 
GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT 
GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG 
GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT 
GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT 
CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG 
CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG 
CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT 
TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG 
GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT 
GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT 
GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGCCT TACTTTACAT CCCTGACTAC 
CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC 
ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA 
AAGAAGAAAA CTAAAAATTC TTCGAAAGCG CAGAAAAACA ACGGATCCAC TTGGGCTAAT 
GTCCCTCTAC CTCCTCCTCC CGTCCAGCCC CTTCCTGGTA CAGAGCTGGG CCACTATGCT 
GCGGAACAAG AAAATGGCTA TGACAGTGAC AGCTGGTGCC CACCATTACC GGTGCAAACA 
TACTTGCATC AGGGTATGGA AGATGAGCTG GAAGAAGACG AAGATCGGGT CCCAACACCT 
CCTGTTCGCG GCGTGGCCTC TTCACCAGCT ATCTCTTTTG GACAGCAGTC CACTGCCACT 
CTTACTCCAT CCCCACGGGA AGAGATGCAA CCCATGCTGC AAGCTCACTT GGATGAGTTG 
ACAAGGGCCT ATCAGTTTGA TATAGCAAAA CAAACATGGC ACATTCAAAG CAATACCCCA 
CCTCCACAAC CCCCAGCTCC GCCATTAGGT TACGTGTCCG GAGCCTTGAT TTCTGATTTG 
GAGACAGATG TTCCAGATGA GGATGCTGAT GATGAAGAGG AACCATTAGA AATTCCCAGG 
CCCCTCAGAG CACTAGACCA GACACCTGGA TCCAGTATGG ACAATCTAGA CAGCTCTGTC 
ACAGGTTCAA TGGTAAATGG ATGGGGCTCT GCATCTGATG AGGATCGTAA CTTTTCTAGT 
CATAGATCTA GTGTAGGTAG CTCCTCAGAT GGCTCCATCT TTGCCAGCGG CAGCTTTGCA 
AAAGCATTGG TGGCAGCAGC AGACAAAGCT GGCTTTAGGC TGGATGGAAC CAGTCTTACG 
AGAACAGGAA AAGCCTTTAG CTCCTCTCAA AGGCAGCGGC CCACCAGCCC ATTTTCTACG 
GACAGTAACA CCAGTGCTGC CCAGAATCAA AGCCAGAGGC CTCGGCCCAC GAAAAAACAT 
AAGGGAGGAC GGATGGACCC ACAGCCAGTG TTACCTCATC GGAGGGAAGG GATGCCAGAT 
GATCTTCCCC CACCACCAGA TCCCCCACCA GGTCAGGGTT TAAGGCAGCA GATAGGCCTG 
AGCCAACACT CTGGTAACGT GGAAAACTCA ACAGAGAGAA AAGGAAGCTC TCTAGAGAGA 
CAACAAGCAG CCAACTTAGA AGACACAAAG AGCTCATTGG ATTGTCCAGC TAAAACTGTT 
CTAGAGTGGC AGCGTCAAAC CCAGGACTGG ATAAACTCTA CAGAACGCCA AGAAGAAACT 
CGGAAAGCCC CACACAAACA AGGGGTTGGA TCAGAGGAGT CCCTGGTGCC CTACAGCAAG 
CCCAGCTTCC CGTCTCCTGG CGGGCACAGC TCATCAGGAA CATCTTCCTC TAAAGGGTCC 
ACTGGCCCTC GGAAGGCTGA CGTGCTGAGA GGCAGCCACC AGAGGAATGC CAATGACCTT 
CTCGACATTG GATATGTGGG CTCAAATAGT CAAGGACAGT TTACAGGTGA ATTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523098.1
CDS	797..5593
Translation	MLSSEKVDKITRLELLFVLMLFFSGPTLGKLTRTPWKGKPWKGGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKAMSTDEGTYVCIAENRVGKVEASATLTVRVRPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPINQTLAVDGTALLKCKATGEPLPVISWLKEGFTFLGRDPRATIQDQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDMNDLPGPPSKPQVTDVSKNSVTLSWQPGTPGVLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVVVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQASTVWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSHNSTSISVSWDPPPADHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVVIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGGRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPNYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTTYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSVQQKTDLMGFGYSLPDQNKGNNALLYIPDYRLAEGLSNRMPHNQSQDFSTTSSHNSSERSGSLSGGKGGKKKKTKNSSKAQKNNGSTWANVPLPPPPVQPLPGTELGHYAAEQENGYDSDSWCPPLPVQTYLHQGMEDELEEDEDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNTPPPQPPAPPLGYVSGALISDLETDVPDEDADDEEEPLEIPRPLRALDQTPGSSMDNLDSSVTGSMVNGWGSASDEDRNFSSHRSSVGSSSDGSIFASGSFAKALVAAADKAGFRLDGTSLTRTGKAFSSSQRQRPTSPFSTDSNTSAAQNQSQRPRPTKKHKGGRMDPQPVLPHRREGMPDDLPPPPDPPPGQGLRQQIGLSQHSGNVENSTERKGSSLERQQAANLEDTKSSLDCPAKTVLEWQRQTQDWINSTERQEETRKAPHKQGVGSEESLVPYSKPSFPSPGGHSSSGTSSSKGSTGPRKADVLRGSHQRNANDLLDIGYVGSNSQGQFTGEL

Target ORF information:

RefSeq Version	XM_006523035.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523035.3

ORF Insert Sequence:

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT 
CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG 
GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC 
CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA 
GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT 
ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG 
GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG 
CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT 
AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT 
TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC 
GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC 
CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT 
ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA 
CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA 
TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA 
GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT 
CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT 
GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG 
CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG 
GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC 
ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG 
GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT 
GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA 
GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT 
GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG 
GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT 
GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT 
CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG 
CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG 
CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT 
TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG 
GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT 
GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT 
GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGCCT TACTTTACAT CCCTGACTAC 
CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC 
ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA 
AAGAAGAAAA CTAAAAATTC TTCGAAAGCG CAGAAAAACA ACGGATCCAC TTGGGCTAAT 
GTCCCTCTAC CTCCTCCTCC CGTCCAGCCC CTTCCTGGTA CAGAGCTGGG CCACTATGCT 
GCGGAACAAG AAAATGGCTA TGACAGTGAC AGCTGGTGCC CACCATTACC GGTGCAAACA 
TACTTGCATC AGGGTATGGA AGATGAGCTG GAAGAAGACG AAGATCGGGT CCCAACACCT 
CCTGTTCGCG GCGTGGCCTC TTCACCAGCT ATCTCTTTTG GACAGCAGTC CACTGCCACT 
CTTACTCCAT CCCCACGGGA AGAGATGCAA CCCATGCTGC AAGCTCACTT GGATGAGTTG 
ACAAGGGCCT ATCAGTTTGA TATAGCAAAA CAAACATGGC ACATTCAAAG CAATACCCCA 
CCTCCACAAC CCCCAGCTCC GCCATTAGGT TACGTGTCCG GAGCCTTGAT TTCTGATTTG 
GAGACAGATG TTCCAGATGA GGATGCTGAT GATGAAGAGG AACCATTAGA AATTCCCAGG 
CCCCTCAGAG CACTAGACCA GACACCTGGA TCCAGTATGG ACAATCTAGA CAGCTCTGTC 
ACAGGTTCAA TGGTAAATGG ATGGGGCTCT GCATCTGATG AGGATCGTAA CTTTTCTAGT 
CATAGATCTA GTGTAGGTAG CTCCTCAGAT GGCTCCATCT TTGCCAGCGG CAGCTTTGCA 
AAAGCATTGG TGGCAGCAGC AGACAAAGCT GGCTTTAGGC TGGATGGAAC CAGTCTTACG 
AGAACAGGAA AAGCCTTTAG CTCCTCTCAA AGGCAGCGGC CCACCAGCCC ATTTTCTACG 
GACAGTAACA CCAGTGCTGC CCAGAATCAA AGCCAGAGGC CTCGGCCCAC GAAAAAACAT 
AAGGGAGGAC GGATGGACCC ACAGCCAGTG TTACCTCATC GGAGGGAAGG GATGCCAGAT 
GATCTTCCCC CACCACCAGA TCCCCCACCA GGTCAGGGTT TAAGGCAGCA GATAGGCCTG 
AGCCAACACT CTGGTAACGT GGAAAACTCA ACAGAGAGAA AAGGAAGCTC TCTAGAGAGA 
CAACAAGCAG CCAACTTAGA AGACACAAAG AGCTCATTGG ATTGTCCAGC TAAAACTGTT 
CTAGAGTGGC AGCGTCAAAC CCAGGACTGG ATAAACTCTA CAGAACGCCA AGAAGAAACT 
CGGAAAGCCC CACACAAACA AGGGGTTGGA TCAGAGGAGT CCCTGGTGCC CTACAGCAAG 
CCCAGCTTCC CGTCTCCTGG CGGGCACAGC TCATCAGGAA CATCTTCCTC TAAAGGGTCC 
ACTGGCCCTC GGAAGGCTGA CGTGCTGAGA GGCAGCCACC AGAGGAATGC CAATGACCTT 
CTCGACATTG GATATGTGGG CTCAAATAGT CAAGGACAGT TTACAGGTGA ATTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo2 ( XM_006523036.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523099.1 Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X2, mRNA.

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CloneID	OMu52756
Clone ID Related Accession (Same CDS sequence)	XM_006523036.3 , XM_006523036.4
Accession Version	XM_006523036.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	roundabout homolog 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006523036.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT 
CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG 
GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC 
CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA 
GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT 
ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG 
GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG 
CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT 
AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT 
TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC 
GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC 
CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT 
ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA 
CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA 
TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA 
GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT 
CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT 
GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG 
CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG 
GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC 
ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG 
GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT 
GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA 
GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT 
GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG 
GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT 
GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT 
CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG 
CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG 
CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT 
TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG 
GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT 
GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT 
GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGCCT TACTTTACAT CCCTGACTAC 
CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC 
ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA 
AAGAAGAAAA CTAAAAATTC TTCGAAAGCG CAGAAAAACA ACGGATCCAC TTGGGCTAAT 
GTCCCTCTAC CTCCTCCTCC CGTCCAGCCC CTTCCTGGTA CAGAGCTGGG CCACTATGCT 
GCGGAACAAG AAAATGGCTA TGACAGTGAC AGCTGGTGCC CACCATTACC GGTGCAAACA 
TACTTGCATC AGGGTATGGA AGATGAGCTG GAAGAAGACG AAGATCGGGT CCCAACACCT 
CCTGTTCGCG GCGTGGCCTC TTCACCAGCT ATCTCTTTTG GACAGCAGTC CACTGCCACT 
CTTACTCCAT CCCCACGGGA AGAGATGCAA CCCATGCTGC AAGCTCACTT GGATGAGTTG 
ACAAGGGCCT ATCAGTTTGA TATAGCAAAA CAAACATGGC ACATTCAAAG CAATACCCCA 
CCTCCACAAC CCCCAGCTCC GCCATTAGGT TACGTGTCCG GAGCCTTGAT TTCTGATTTG 
GAGACAGATG TTCCAGATGA GGATGCTGAT GATGAAGAGG AACCATTAGA AATTCCCAGG 
CCCCTCAGAG CACTAGACCA GACACCTGGA TCCAGTATGG ACAATCTAGA CAGCTCTGTC 
ACAGGTTCAA TGGTAAATGG ATGGGGCTCT GCATCTGATG AGGATCGTAA CTTTTCTAGT 
CATAGATCTA GTGTAGGTAG CTCCTCAGAT GGCTCCATCT TTGCCAGCGG CAGCTTTGCA 
AAAGCATTGG TGGCAGCAGC AGACAAAGCT GGCTTTAGGC TGGATGGAAC CAGTCTTACG 
AGAACAGGAA AAGCCTTTAG CTCCTCTCAA AGGCAGCGGC CCACCAGCCC ATTTTCTACG 
GACAGTAACA CCAGTGCTGC CCAGAATCAA AGCCAGAGGC CTCGGCCCAC GAAAAAACAT 
AAGGGAGGAC GGATGGACCC ACAGCCAGTG TTACCTCATC GGAGGGAAGG GATGCCAGAT 
GATCTTCCCC CACCACCAGA TCCCCCACCA GGTCAGGGTT TAAGGCAGCA GATAGGCCTG 
AGCCAACACT CTGGTAACGT GGAAAACTCA ACAGAGAGAA AAGGAAGCTC TCTAGAGAGA 
CAACAAGCAG CCAACTTAGA AGACACAAAG AGCTCATTGG ATTGTCCAGC TAAAACTGTT 
CTAGAGTGGC AGCGTCAAAC CCAGGACTGG ATAAACTCTA CAGAACGCCA AGAAGAAACT 
CGGAAAGCCC CACACAAACA AGGGGTTGGA TCAGAGGAGT CCCTGGTGCC CTACAGCAAG 
CCCAGCTTCC CGTCTCCTGG CGGGCACAGC TCATCAGGAA CATCTTCCTC TAAAGGGTCC 
ACTGGCCCTC GGAAGGCTGA CGTGCTGAGA GGCAGCCACC AGAGGAATGC CAATGACCTT 
CTCGACATTG GATATGTGGG CTCAAATAGT CAAGGACAGT TTACAGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523099.1
CDS	337..5127
Translation	MLSSEKVDKITRLELLFVLMLFFSGPTLGKLTRTPWKGKPWKGGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKAMSTDEGTYVCIAENRVGKVEASATLTVRVRPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPINQTLAVDGTALLKCKATGEPLPVISWLKEGFTFLGRDPRATIQDQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDMNDLPGPPSKPQVTDVSKNSVTLSWQPGTPGVLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVVVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQASTVWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSHNSTSISVSWDPPPADHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVVIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGGRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPNYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTTYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSVQQKTDLMGFGYSLPDQNKGNNALLYIPDYRLAEGLSNRMPHNQSQDFSTTSSHNSSERSGSLSGGKGGKKKKTKNSSKAQKNNGSTWANVPLPPPPVQPLPGTELGHYAAEQENGYDSDSWCPPLPVQTYLHQGMEDELEEDEDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNTPPPQPPAPPLGYVSGALISDLETDVPDEDADDEEEPLEIPRPLRALDQTPGSSMDNLDSSVTGSMVNGWGSASDEDRNFSSHRSSVGSSSDGSIFASGSFAKALVAAADKAGFRLDGTSLTRTGKAFSSSQRQRPTSPFSTDSNTSAAQNQSQRPRPTKKHKGGRMDPQPVLPHRREGMPDDLPPPPDPPPGQGLRQQIGLSQHSGNVENSTERKGSSLERQQAANLEDTKSSLDCPAKTVLEWQRQTQDWINSTERQEETRKAPHKQGVGSEESLVPYSKPSFPSPGGHSSSGTSSSKGSTGPRKADVLRGSHQRNANDLLDIGYVGSNSQGQFTE

Target ORF information:

RefSeq Version	XM_006523036.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523036.3

ORF Insert Sequence:

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT 
CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG 
GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC 
CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA 
GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT 
ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG 
GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG 
CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT 
AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT 
TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC 
GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC 
CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT 
ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA 
CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA 
TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA 
GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT 
CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT 
GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG 
CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG 
GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC 
ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG 
GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT 
GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA 
GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT 
GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG 
GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT 
GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT 
CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG 
CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG 
CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT 
TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG 
GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT 
GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT 
GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGCCT TACTTTACAT CCCTGACTAC 
CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC 
ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA 
AAGAAGAAAA CTAAAAATTC TTCGAAAGCG CAGAAAAACA ACGGATCCAC TTGGGCTAAT 
GTCCCTCTAC CTCCTCCTCC CGTCCAGCCC CTTCCTGGTA CAGAGCTGGG CCACTATGCT 
GCGGAACAAG AAAATGGCTA TGACAGTGAC AGCTGGTGCC CACCATTACC GGTGCAAACA 
TACTTGCATC AGGGTATGGA AGATGAGCTG GAAGAAGACG AAGATCGGGT CCCAACACCT 
CCTGTTCGCG GCGTGGCCTC TTCACCAGCT ATCTCTTTTG GACAGCAGTC CACTGCCACT 
CTTACTCCAT CCCCACGGGA AGAGATGCAA CCCATGCTGC AAGCTCACTT GGATGAGTTG 
ACAAGGGCCT ATCAGTTTGA TATAGCAAAA CAAACATGGC ACATTCAAAG CAATACCCCA 
CCTCCACAAC CCCCAGCTCC GCCATTAGGT TACGTGTCCG GAGCCTTGAT TTCTGATTTG 
GAGACAGATG TTCCAGATGA GGATGCTGAT GATGAAGAGG AACCATTAGA AATTCCCAGG 
CCCCTCAGAG CACTAGACCA GACACCTGGA TCCAGTATGG ACAATCTAGA CAGCTCTGTC 
ACAGGTTCAA TGGTAAATGG ATGGGGCTCT GCATCTGATG AGGATCGTAA CTTTTCTAGT 
CATAGATCTA GTGTAGGTAG CTCCTCAGAT GGCTCCATCT TTGCCAGCGG CAGCTTTGCA 
AAAGCATTGG TGGCAGCAGC AGACAAAGCT GGCTTTAGGC TGGATGGAAC CAGTCTTACG 
AGAACAGGAA AAGCCTTTAG CTCCTCTCAA AGGCAGCGGC CCACCAGCCC ATTTTCTACG 
GACAGTAACA CCAGTGCTGC CCAGAATCAA AGCCAGAGGC CTCGGCCCAC GAAAAAACAT 
AAGGGAGGAC GGATGGACCC ACAGCCAGTG TTACCTCATC GGAGGGAAGG GATGCCAGAT 
GATCTTCCCC CACCACCAGA TCCCCCACCA GGTCAGGGTT TAAGGCAGCA GATAGGCCTG 
AGCCAACACT CTGGTAACGT GGAAAACTCA ACAGAGAGAA AAGGAAGCTC TCTAGAGAGA 
CAACAAGCAG CCAACTTAGA AGACACAAAG AGCTCATTGG ATTGTCCAGC TAAAACTGTT 
CTAGAGTGGC AGCGTCAAAC CCAGGACTGG ATAAACTCTA CAGAACGCCA AGAAGAAACT 
CGGAAAGCCC CACACAAACA AGGGGTTGGA TCAGAGGAGT CCCTGGTGCC CTACAGCAAG 
CCCAGCTTCC CGTCTCCTGG CGGGCACAGC TCATCAGGAA CATCTTCCTC TAAAGGGTCC 
ACTGGCCCTC GGAAGGCTGA CGTGCTGAGA GGCAGCCACC AGAGGAATGC CAATGACCTT 
CTCGACATTG GATATGTGGG CTCAAATAGT CAAGGACAGT TTACAGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo2 ( XM_006523037.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523100.1 Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu52757
Clone ID Related Accession (Same CDS sequence)	XM_006523037.4 , XM_006523037.3
Accession Version	XM_006523037.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4785bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	roundabout homolog 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006523037.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGCTCCTCC ACAGTTTGTG 
GTTAGGCCAA GAGATCAGAT CGTTGCTCAA GGCCGAACAG TGACATTCCC CTGTGAAACT 
AAAGGAAACC CACAGCCAGC TGTTTTTTGG CAGAAAGAAG GCAGCCAGAA CCTACTTTTC 
CCGAATCAAC CTCAGCAGCC CAACAGCCGA TGTTCAGTGT CGCCCACGGG GGACCTCACC 
ATCACCAACA TCCAGCGTTC AGATGCGGGT TACTACATCT GCCAGGCCCT AACCGTGGCA 
GGAAGCATTT TAGCTAAAGC ACAGTTGGAA GTTACTGACG TTTTGACAGA TAGACCTCCA 
CCCATAATCT TGCAAGGACC AATAAACCAA ACACTTGCAG TAGACGGTAC AGCATTGTTG 
AAGTGTAAAG CCACTGGTGA GCCTCTGCCT GTAATTAGCT GGCTAAAGGA GGGCTTTACT 
TTTCTGGGGA GAGATCCAAG AGCCACGATC CAAGACCAAG GAACACTGCA GATTAAGAAT 
TTACGGATAT CTGATACTGG CACTTATACT TGTGTGGCTA CAAGTTCCAG TGGAGAGACT 
TCCTGGAGTG CAGTGCTGGA TGTAACAGAA TCTGGAGCAA CAATCAGTAA AAATTATGAT 
ATGAATGACC TCCCGGGACC ACCATCCAAA CCTCAGGTCA CTGATGTTTC TAAGAACAGT 
GTCACCTTAT CCTGGCAGCC AGGTACACCT GGCGTTCTTC CTGCAAGCGC GTATATCATT 
GAGGCTTTCA GCCAATCGGT GAGCAATAGC TGGCAGACAG TGGCAAACCA TGTTAAGACA 
ACTCTGTATA CAGTAAGGGG GCTGAGGCCC AACACAATCT ACTTGTTTAT GGTCAGAGCG 
ATCAACCCAC AAGGTCTCAG TGATCCAAGT CCTATGTCGG ATCCTGTACG CACACAAGAT 
ATCAGCCCCC CAGCACAAGG AGTGGACCAC AGACAGGTGC AGAAGGAATT AGGTGATGTC 
GTTGTTCGTC TCCATAATCC AGTTGTCCTG ACACCTACAA CTGTTCAAGT CACATGGACG 
GTGGACCGAC AACCCCAGTT TATTCAGGGC TACAGAGTGA TGTACCGTCA GACTTCGGGA 
CTACAAGCCT CAACTGTGTG GCAGAATCTA GACGCCAAAG TCCCGACTGA GAGGAGTGCT 
GTCCTTGTGA ATTTGAAAAA GGGGGTGACT TATGAAATTA AAGTCCGGCC GTATTTTAAC 
GAGTTCCAAG GAATGGACAG TGAATCGAAA ACAGTCCGAA CCACTGAGGA AGCCCCAAGT 
GCCCCTCCCC AGTCTGTCAC TGTGCTGACA GTTGGAAGTC ACAACAGCAC AAGCATCAGT 
GTTTCCTGGG ATCCTCCACC AGCCGACCAC CAGAATGGAA TTATTCAGGA ATATAAGATC 
TGGTGTCTGG GAAACGAAAC GCGATTCCAT ATCAATAAAA CGGTGGATGC AGCCATTCGC 
TCTGTAGTAA TAGGTGGCTT GTTCCCTGGA ATTCAGTACC GGGTAGAAGT GGCAGCTAGC 
ACAAGTGCAG GGGTTGGAGT AAAAAGTGAA CCACAGCCGA TAATAATTGG GGGACGTAAC 
GAAGTTGTCA TTACTGAAAA CAATAACAGC ATCACTGAGC AAATCACGGA TGTCGTGAAG 
CAACCGGCAT TTATAGCTGG CATTGGTGGT GCCTGCTGGG TAATTCTGAT GGGTTTTAGC 
ATCTGGTTGT ACTGGAGAAG AAAGAAGAGA AAGGGACTCA GCAATTATGC TGTAACATTT 
CAAAGAGGAG ATGGAGGACT AATGAGCAAT GGGAGCCGTC CAGGTCTTCT AAATGCTGGC 
GATCCCAATT ACCCATGGCT TGCTGATTCT TGGCCAGCCA CGAGTTTGCC AGTGAACAAT 
AGCAATAGTG GCCCAAATGA AATTGGAAAT TTTGGGCGTG GAGATGTGCT GCCTCCGGTG 
CCAGGCCAAG GGGATAAAAC AGCGACCATG CTCTCGGATG GAGCCATTTA TAGCAGCATT 
GACTTCACTA CCAAAACCAC TTACAACAGT TCCAGCCAAA TAACACAGGC CACCCCATAT 
GCCACTACAC AAATCCTGCA TTCAAACAGC ATCCACGAAC TGGCAGTTGA TCTTCCTGAT 
CCACAGTGGA AAAGCTCAGT TCAACAGAAG ACAGACCTCA TGGGATTTGG TTATTCGCTA 
CCTGATCAGA ACAAGGGGAA CAACGCCTTA CTTTACATCC CTGACTACCG ATTGGCTGAG 
GGATTGTCTA ATAGAATGCC ACACAACCAG TCACAGGATT TCAGCACCAC CAGCTCTCAC 
AACAGCTCAG AAAGGAGTGG CAGTCTCTCA GGTGGGAAAG GTGGAAAAAA GAAGAAAACT 
AAAAATTCTT CGAAAGCGCA GAAAAACAAC GGATCCACTT GGGCTAATGT CCCTCTACCT 
CCTCCTCCCG TCCAGCCCCT TCCTGGTACA GAGCTGGGCC ACTATGCTGC GGAACAAGAA 
AATGGCTATG ACAGTGACAG CTGGTGCCCA CCATTACCGG TGCAAACATA CTTGCATCAG 
GGTATGGAAG ATGAGCTGGA AGAAGACGAA GATCGGGTCC CAACACCTCC TGTTCGCGGC 
GTGGCCTCTT CACCAGCTAT CTCTTTTGGA CAGCAGTCCA CTGCCACTCT TACTCCATCC 
CCACGGGAAG AGATGCAACC CATGCTGCAA GCTCACTTGG ATGAGTTGAC AAGGGCCTAT 
CAGTTTGATA TAGCAAAACA AACATGGCAC ATTCAAAGCA ATACCCCACC TCCACAACCC 
CCAGCTCCGC CATTAGGTTA CGTGTCCGGA GCCTTGATTT CTGATTTGGA GACAGATGTT 
CCAGATGAGG ATGCTGATGA TGAAGAGGAA CCATTAGAAA TTCCCAGGCC CCTCAGAGCA 
CTAGACCAGA CACCTGGATC CAGTATGGAC AATCTAGACA GCTCTGTCAC AGGTTCAATG 
GTAAATGGAT GGGGCTCTGC ATCTGATGAG GATCGTAACT TTTCTAGTCA TAGATCTAGT 
GTAGGTAGCT CCTCAGATGG CTCCATCTTT GCCAGCGGCA GCTTTGCAAA AGCATTGGTG 
GCAGCAGCAG ACAAAGCTGG CTTTAGGCTG GATGGAACCA GTCTTACGAG AACAGGAAAA 
GCCTTTAGCT CCTCTCAAAG GCAGCGGCCC ACCAGCCCAT TTTCTACGGA CAGTAACACC 
AGTGCTGCCC AGAATCAAAG CCAGAGGCCT CGGCCCACGA AAAAACATAA GGGAGGACGG 
ATGGACCCAC AGCCAGTGTT ACCTCATCGG AGGGAAGGGA TGCCAGATGA TCTTCCCCCA 
CCACCAGATC CCCCACCAGG TCAGGGTTTA AGGCAGCAGA TAGGCCTGAG CCAACACTCT 
GGTAACGTGG AAAACTCAAC AGAGAGAAAA GGAAGCTCTC TAGAGAGACA ACAAGCAGCC 
AACTTAGAAG ACACAAAGAG CTCATTGGAT TGTCCAGCTA AAACTGTTCT AGAGTGGCAG 
CGTCAAACCC AGGACTGGAT AAACTCTACA GAACGCCAAG AAGAAACTCG GAAAGCCCCA 
CACAAACAAG GGGTTGGATC AGAGGAGTCC CTGGTGCCCT ACAGCAAGCC CAGCTTCCCG 
TCTCCTGGCG GGCACAGCTC ATCAGGAACA TCTTCCTCTA AAGGGTCCAC TGGCCCTCGG 
AAGGCTGACG TGCTGAGAGG CAGCCACCAG AGGAATGCCA ATGACCTTCT CGACATTGGA 
TATGTGGGCT CAAATAGTCA AGGACAGTTT ACAGGTGAAT TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523100.1
CDS	337..5121
Translation	MLSSEKVDKITRLELLFVLMLFFSGPTLGKLTRTPWKGKPWKGGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKAMSTDEGTYVCIAENRVGKVEASATLTVRAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPINQTLAVDGTALLKCKATGEPLPVISWLKEGFTFLGRDPRATIQDQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDMNDLPGPPSKPQVTDVSKNSVTLSWQPGTPGVLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVVVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQASTVWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSHNSTSISVSWDPPPADHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVVIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGGRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPNYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTTYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSVQQKTDLMGFGYSLPDQNKGNNALLYIPDYRLAEGLSNRMPHNQSQDFSTTSSHNSSERSGSLSGGKGGKKKKTKNSSKAQKNNGSTWANVPLPPPPVQPLPGTELGHYAAEQENGYDSDSWCPPLPVQTYLHQGMEDELEEDEDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNTPPPQPPAPPLGYVSGALISDLETDVPDEDADDEEEPLEIPRPLRALDQTPGSSMDNLDSSVTGSMVNGWGSASDEDRNFSSHRSSVGSSSDGSIFASGSFAKALVAAADKAGFRLDGTSLTRTGKAFSSSQRQRPTSPFSTDSNTSAAQNQSQRPRPTKKHKGGRMDPQPVLPHRREGMPDDLPPPPDPPPGQGLRQQIGLSQHSGNVENSTERKGSSLERQQAANLEDTKSSLDCPAKTVLEWQRQTQDWINSTERQEETRKAPHKQGVGSEESLVPYSKPSFPSPGGHSSSGTSSSKGSTGPRKADVLRGSHQRNANDLLDIGYVGSNSQGQFTGEL

Target ORF information:

RefSeq Version	XM_006523037.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523037.3

ORF Insert Sequence:

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGCTCCTCC ACAGTTTGTG 
GTTAGGCCAA GAGATCAGAT CGTTGCTCAA GGCCGAACAG TGACATTCCC CTGTGAAACT 
AAAGGAAACC CACAGCCAGC TGTTTTTTGG CAGAAAGAAG GCAGCCAGAA CCTACTTTTC 
CCGAATCAAC CTCAGCAGCC CAACAGCCGA TGTTCAGTGT CGCCCACGGG GGACCTCACC 
ATCACCAACA TCCAGCGTTC AGATGCGGGT TACTACATCT GCCAGGCCCT AACCGTGGCA 
GGAAGCATTT TAGCTAAAGC ACAGTTGGAA GTTACTGACG TTTTGACAGA TAGACCTCCA 
CCCATAATCT TGCAAGGACC AATAAACCAA ACACTTGCAG TAGACGGTAC AGCATTGTTG 
AAGTGTAAAG CCACTGGTGA GCCTCTGCCT GTAATTAGCT GGCTAAAGGA GGGCTTTACT 
TTTCTGGGGA GAGATCCAAG AGCCACGATC CAAGACCAAG GAACACTGCA GATTAAGAAT 
TTACGGATAT CTGATACTGG CACTTATACT TGTGTGGCTA CAAGTTCCAG TGGAGAGACT 
TCCTGGAGTG CAGTGCTGGA TGTAACAGAA TCTGGAGCAA CAATCAGTAA AAATTATGAT 
ATGAATGACC TCCCGGGACC ACCATCCAAA CCTCAGGTCA CTGATGTTTC TAAGAACAGT 
GTCACCTTAT CCTGGCAGCC AGGTACACCT GGCGTTCTTC CTGCAAGCGC GTATATCATT 
GAGGCTTTCA GCCAATCGGT GAGCAATAGC TGGCAGACAG TGGCAAACCA TGTTAAGACA 
ACTCTGTATA CAGTAAGGGG GCTGAGGCCC AACACAATCT ACTTGTTTAT GGTCAGAGCG 
ATCAACCCAC AAGGTCTCAG TGATCCAAGT CCTATGTCGG ATCCTGTACG CACACAAGAT 
ATCAGCCCCC CAGCACAAGG AGTGGACCAC AGACAGGTGC AGAAGGAATT AGGTGATGTC 
GTTGTTCGTC TCCATAATCC AGTTGTCCTG ACACCTACAA CTGTTCAAGT CACATGGACG 
GTGGACCGAC AACCCCAGTT TATTCAGGGC TACAGAGTGA TGTACCGTCA GACTTCGGGA 
CTACAAGCCT CAACTGTGTG GCAGAATCTA GACGCCAAAG TCCCGACTGA GAGGAGTGCT 
GTCCTTGTGA ATTTGAAAAA GGGGGTGACT TATGAAATTA AAGTCCGGCC GTATTTTAAC 
GAGTTCCAAG GAATGGACAG TGAATCGAAA ACAGTCCGAA CCACTGAGGA AGCCCCAAGT 
GCCCCTCCCC AGTCTGTCAC TGTGCTGACA GTTGGAAGTC ACAACAGCAC AAGCATCAGT 
GTTTCCTGGG ATCCTCCACC AGCCGACCAC CAGAATGGAA TTATTCAGGA ATATAAGATC 
TGGTGTCTGG GAAACGAAAC GCGATTCCAT ATCAATAAAA CGGTGGATGC AGCCATTCGC 
TCTGTAGTAA TAGGTGGCTT GTTCCCTGGA ATTCAGTACC GGGTAGAAGT GGCAGCTAGC 
ACAAGTGCAG GGGTTGGAGT AAAAAGTGAA CCACAGCCGA TAATAATTGG GGGACGTAAC 
GAAGTTGTCA TTACTGAAAA CAATAACAGC ATCACTGAGC AAATCACGGA TGTCGTGAAG 
CAACCGGCAT TTATAGCTGG CATTGGTGGT GCCTGCTGGG TAATTCTGAT GGGTTTTAGC 
ATCTGGTTGT ACTGGAGAAG AAAGAAGAGA AAGGGACTCA GCAATTATGC TGTAACATTT 
CAAAGAGGAG ATGGAGGACT AATGAGCAAT GGGAGCCGTC CAGGTCTTCT AAATGCTGGC 
GATCCCAATT ACCCATGGCT TGCTGATTCT TGGCCAGCCA CGAGTTTGCC AGTGAACAAT 
AGCAATAGTG GCCCAAATGA AATTGGAAAT TTTGGGCGTG GAGATGTGCT GCCTCCGGTG 
CCAGGCCAAG GGGATAAAAC AGCGACCATG CTCTCGGATG GAGCCATTTA TAGCAGCATT 
GACTTCACTA CCAAAACCAC TTACAACAGT TCCAGCCAAA TAACACAGGC CACCCCATAT 
GCCACTACAC AAATCCTGCA TTCAAACAGC ATCCACGAAC TGGCAGTTGA TCTTCCTGAT 
CCACAGTGGA AAAGCTCAGT TCAACAGAAG ACAGACCTCA TGGGATTTGG TTATTCGCTA 
CCTGATCAGA ACAAGGGGAA CAACGCCTTA CTTTACATCC CTGACTACCG ATTGGCTGAG 
GGATTGTCTA ATAGAATGCC ACACAACCAG TCACAGGATT TCAGCACCAC CAGCTCTCAC 
AACAGCTCAG AAAGGAGTGG CAGTCTCTCA GGTGGGAAAG GTGGAAAAAA GAAGAAAACT 
AAAAATTCTT CGAAAGCGCA GAAAAACAAC GGATCCACTT GGGCTAATGT CCCTCTACCT 
CCTCCTCCCG TCCAGCCCCT TCCTGGTACA GAGCTGGGCC ACTATGCTGC GGAACAAGAA 
AATGGCTATG ACAGTGACAG CTGGTGCCCA CCATTACCGG TGCAAACATA CTTGCATCAG 
GGTATGGAAG ATGAGCTGGA AGAAGACGAA GATCGGGTCC CAACACCTCC TGTTCGCGGC 
GTGGCCTCTT CACCAGCTAT CTCTTTTGGA CAGCAGTCCA CTGCCACTCT TACTCCATCC 
CCACGGGAAG AGATGCAACC CATGCTGCAA GCTCACTTGG ATGAGTTGAC AAGGGCCTAT 
CAGTTTGATA TAGCAAAACA AACATGGCAC ATTCAAAGCA ATACCCCACC TCCACAACCC 
CCAGCTCCGC CATTAGGTTA CGTGTCCGGA GCCTTGATTT CTGATTTGGA GACAGATGTT 
CCAGATGAGG ATGCTGATGA TGAAGAGGAA CCATTAGAAA TTCCCAGGCC CCTCAGAGCA 
CTAGACCAGA CACCTGGATC CAGTATGGAC AATCTAGACA GCTCTGTCAC AGGTTCAATG 
GTAAATGGAT GGGGCTCTGC ATCTGATGAG GATCGTAACT TTTCTAGTCA TAGATCTAGT 
GTAGGTAGCT CCTCAGATGG CTCCATCTTT GCCAGCGGCA GCTTTGCAAA AGCATTGGTG 
GCAGCAGCAG ACAAAGCTGG CTTTAGGCTG GATGGAACCA GTCTTACGAG AACAGGAAAA 
GCCTTTAGCT CCTCTCAAAG GCAGCGGCCC ACCAGCCCAT TTTCTACGGA CAGTAACACC 
AGTGCTGCCC AGAATCAAAG CCAGAGGCCT CGGCCCACGA AAAAACATAA GGGAGGACGG 
ATGGACCCAC AGCCAGTGTT ACCTCATCGG AGGGAAGGGA TGCCAGATGA TCTTCCCCCA 
CCACCAGATC CCCCACCAGG TCAGGGTTTA AGGCAGCAGA TAGGCCTGAG CCAACACTCT 
GGTAACGTGG AAAACTCAAC AGAGAGAAAA GGAAGCTCTC TAGAGAGACA ACAAGCAGCC 
AACTTAGAAG ACACAAAGAG CTCATTGGAT TGTCCAGCTA AAACTGTTCT AGAGTGGCAG 
CGTCAAACCC AGGACTGGAT AAACTCTACA GAACGCCAAG AAGAAACTCG GAAAGCCCCA 
CACAAACAAG GGGTTGGATC AGAGGAGTCC CTGGTGCCCT ACAGCAAGCC CAGCTTCCCG 
TCTCCTGGCG GGCACAGCTC ATCAGGAACA TCTTCCTCTA AAGGGTCCAC TGGCCCTCGG 
AAGGCTGACG TGCTGAGAGG CAGCCACCAG AGGAATGCCA ATGACCTTCT CGACATTGGA 
TATGTGGGCT CAAATAGTCA AGGACAGTTT ACAGGTGAAT TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo2 ( XM_006523038.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523101.1 Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu52758
Clone ID Related Accession (Same CDS sequence)	XM_006523038.3 , XM_006523038.4 , XM_030249140.1 , XM_030249139.1
Accession Version	XM_006523038.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4773bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	roundabout homolog 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006523038.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGAA GACAAGAAAG TATCTGTGGA AGGCCGTGGA CGTGGACGCC AGGACTACTG 
ATGCTGATCA TCCTTGGCAT TCATCAGGGG AGTGGGCAAG GTCAAGGATC TCGTCTTCGT 
CAAGAAGACT TTCCCCCCAG GATCGTAGAA CACCCTTCTG ATGTCATCGT CTCCAAGGGA 
GAGCCCACCA CTCTGAACTG TAAAGCAGAG GGCCGACCCA CCCCCACCAT TGAATGGTAC 
AAGGATGGTG AGAGGGTGGA GACAGACAAG GATGATCCCA GGTCCCACAG AATGCTTCTG 
CCCAGCGGAT CTTTATTCTT TTTGCGAATT GTTCATGGGC GCAGAAGTAA ACCGGACGAA 
GGGAGTTACG TTTGTGTTGC AAGGAACTAT CTTGGTGAAG CAGTGAGTCG AAATGCATCT 
CTGGAAGTGG CATTATTGCG AGATGACTTC CGGCAAAACC CCACAGATGT GGTAGTCGCA 
GCTGGAGAGC CTGCAATCTT GGAGTGCCAG CCACCACGGG GACACCCAGA ACCAACCATC 
TACTGGAAAA AGGACAAAGT CCGAATTGAT GACAAGGAAG AGAGAATAAG TATCCGTGGT 
GGGAAGCTGA TGATCTCTAA TACTAGGAAA AGCGATGCTG GCATGTACAC CTGTGTGGGA 
ACCAATATGG TGGGAGAAAG GGACAGCGAC CCTGCAGAGC TCACTGTCTT TGAACGACCC 
ACATTTCTCA GGAGGCCAAT TAACCAGGTG GTGCTAGAGG AAGAAGCTGT AGAATTCCGT 
TGTCAGGTCC AAGGAGATCC CCAGCCAACG GTGAGGTGGA AAAAAGATGA TGCAGACTTG 
CCGAGAGGAA GGTATGATAT CAAAGATGAC TACACGCTGA GAATTAAAAA GGCCATGAGT 
ACTGATGAAG GTACCTATGT GTGTATTGCT GAGAATCGGG TGGGAAAAGT GGAAGCCTCT 
GCTACCCTCA CTGTCCGAGT TCGCCCTGTT GCTCCTCCAC AGTTTGTGGT TAGGCCAAGA 
GATCAGATCG TTGCTCAAGG CCGAACAGTG ACATTCCCCT GTGAAACTAA AGGAAACCCA 
CAGCCAGCTG TTTTTTGGCA GAAAGAAGGC AGCCAGAACC TACTTTTCCC GAATCAACCT 
CAGCAGCCCA ACAGCCGATG TTCAGTGTCG CCCACGGGGG ACCTCACCAT CACCAACATC 
CAGCGTTCAG ATGCGGGTTA CTACATCTGC CAGGCCCTAA CCGTGGCAGG AAGCATTTTA 
GCTAAAGCAC AGTTGGAAGT TACTGACGTT TTGACAGATA GACCTCCACC CATAATCTTG 
CAAGGACCAA TAAACCAAAC ACTTGCAGTA GACGGTACAG CATTGTTGAA GTGTAAAGCC 
ACTGGTGAGC CTCTGCCTGT AATTAGCTGG CTAAAGGAGG GCTTTACTTT TCTGGGGAGA 
GATCCAAGAG CCACGATCCA AGACCAAGGA ACACTGCAGA TTAAGAATTT ACGGATATCT 
GATACTGGCA CTTATACTTG TGTGGCTACA AGTTCCAGTG GAGAGACTTC CTGGAGTGCA 
GTGCTGGATG TAACAGAATC TGGAGCAACA ATCAGTAAAA ATTATGATAT GAATGACCTC 
CCGGGACCAC CATCCAAACC TCAGGTCACT GATGTTTCTA AGAACAGTGT CACCTTATCC 
TGGCAGCCAG GTACACCTGG CGTTCTTCCT GCAAGCGCGT ATATCATTGA GGCTTTCAGC 
CAATCGGTGA GCAATAGCTG GCAGACAGTG GCAAACCATG TTAAGACAAC TCTGTATACA 
GTAAGGGGGC TGAGGCCCAA CACAATCTAC TTGTTTATGG TCAGAGCGAT CAACCCACAA 
GGTCTCAGTG ATCCAAGTCC TATGTCGGAT CCTGTACGCA CACAAGATAT CAGCCCCCCA 
GCACAAGGAG TGGACCACAG ACAGGTGCAG AAGGAATTAG GTGATGTCGT TGTTCGTCTC 
CATAATCCAG TTGTCCTGAC ACCTACAACT GTTCAAGTCA CATGGACGGT GGACCGACAA 
CCCCAGTTTA TTCAGGGCTA CAGAGTGATG TACCGTCAGA CTTCGGGACT ACAAGCCTCA 
ACTGTGTGGC AGAATCTAGA CGCCAAAGTC CCGACTGAGA GGAGTGCTGT CCTTGTGAAT 
TTGAAAAAGG GGGTGACTTA TGAAATTAAA GTCCGGCCGT ATTTTAACGA GTTCCAAGGA 
ATGGACAGTG AATCGAAAAC AGTCCGAACC ACTGAGGAAG CCCCAAGTGC CCCTCCCCAG 
TCTGTCACTG TGCTGACAGT TGGAAGTCAC AACAGCACAA GCATCAGTGT TTCCTGGGAT 
CCTCCACCAG CCGACCACCA GAATGGAATT ATTCAGGAAT ATAAGATCTG GTGTCTGGGA 
AACGAAACGC GATTCCATAT CAATAAAACG GTGGATGCAG CCATTCGCTC TGTAGTAATA 
GGTGGCTTGT TCCCTGGAAT TCAGTACCGG GTAGAAGTGG CAGCTAGCAC AAGTGCAGGG 
GTTGGAGTAA AAAGTGAACC ACAGCCGATA ATAATTGGGG GACGTAACGA AGTTGTCATT 
ACTGAAAACA ATAACAGCAT CACTGAGCAA ATCACGGATG TCGTGAAGCA ACCGGCATTT 
ATAGCTGGCA TTGGTGGTGC CTGCTGGGTA ATTCTGATGG GTTTTAGCAT CTGGTTGTAC 
TGGAGAAGAA AGAAGAGAAA GGGACTCAGC AATTATGCTG TAACATTTCA AAGAGGAGAT 
GGAGGACTAA TGAGCAATGG GAGCCGTCCA GGTCTTCTAA ATGCTGGCGA TCCCAATTAC 
CCATGGCTTG CTGATTCTTG GCCAGCCACG AGTTTGCCAG TGAACAATAG CAATAGTGGC 
CCAAATGAAA TTGGAAATTT TGGGCGTGGA GATGTGCTGC CTCCGGTGCC AGGCCAAGGG 
GATAAAACAG CGACCATGCT CTCGGATGGA GCCATTTATA GCAGCATTGA CTTCACTACC 
AAAACCACTT ACAACAGTTC CAGCCAAATA ACACAGGCCA CCCCATATGC CACTACACAA 
ATCCTGCATT CAAACAGCAT CCACGAACTG GCAGTTGATC TTCCTGATCC ACAGTGGAAA 
AGCTCAGTTC AACAGAAGAC AGACCTCATG GGATTTGGTT ATTCGCTACC TGATCAGAAC 
AAGGGGAACA ACGCCTTACT TTACATCCCT GACTACCGAT TGGCTGAGGG ATTGTCTAAT 
AGAATGCCAC ACAACCAGTC ACAGGATTTC AGCACCACCA GCTCTCACAA CAGCTCAGAA 
AGGAGTGGCA GTCTCTCAGG TGGGAAAGGT GGAAAAAAGA AGAAAACTAA AAATTCTTCG 
AAAGCGCAGA AAAACAACGG ATCCACTTGG GCTAATGTCC CTCTACCTCC TCCTCCCGTC 
CAGCCCCTTC CTGGTACAGA GCTGGGCCAC TATGCTGCGG AACAAGAAAA TGGCTATGAC 
AGTGACAGCT GGTGCCCACC ATTACCGGTG CAAACATACT TGCATCAGGG TATGGAAGAT 
GAGCTGGAAG AAGACGAAGA TCGGGTCCCA ACACCTCCTG TTCGCGGCGT GGCCTCTTCA 
CCAGCTATCT CTTTTGGACA GCAGTCCACT GCCACTCTTA CTCCATCCCC ACGGGAAGAG 
ATGCAACCCA TGCTGCAAGC TCACTTGGAT GAGTTGACAA GGGCCTATCA GTTTGATATA 
GCAAAACAAA CATGGCACAT TCAAAGCAAT ACCCCACCTC CACAACCCCC AGCTCCGCCA 
TTAGGTTACG TGTCCGGAGC CTTGATTTCT GATTTGGAGA CAGATGTTCC AGATGAGGAT 
GCTGATGATG AAGAGGAACC ATTAGAAATT CCCAGGCCCC TCAGAGCACT AGACCAGACA 
CCTGGATCCA GTATGGACAA TCTAGACAGC TCTGTCACAG GTTCAATGGT AAATGGATGG 
GGCTCTGCAT CTGATGAGGA TCGTAACTTT TCTAGTCATA GATCTAGTGT AGGTAGCTCC 
TCAGATGGCT CCATCTTTGC CAGCGGCAGC TTTGCAAAAG CATTGGTGGC AGCAGCAGAC 
AAAGCTGGCT TTAGGCTGGA TGGAACCAGT CTTACGAGAA CAGGAAAAGC CTTTAGCTCC 
TCTCAAAGGC AGCGGCCCAC CAGCCCATTT TCTACGGACA GTAACACCAG TGCTGCCCAG 
AATCAAAGCC AGAGGCCTCG GCCCACGAAA AAACATAAGG GAGGACGGAT GGACCCACAG 
CCAGTGTTAC CTCATCGGAG GGAAGGGATG CCAGATGATC TTCCCCCACC ACCAGATCCC 
CCACCAGGTC AGGGTTTAAG GCAGCAGATA GGCCTGAGCC AACACTCTGG TAACGTGGAA 
AACTCAACAG AGAGAAAAGG AAGCTCTCTA GAGAGACAAC AAGCAGCCAA CTTAGAAGAC 
ACAAAGAGCT CATTGGATTG TCCAGCTAAA ACTGTTCTAG AGTGGCAGCG TCAAACCCAG 
GACTGGATAA ACTCTACAGA ACGCCAAGAA GAAACTCGGA AAGCCCCACA CAAACAAGGG 
GTTGGATCAG AGGAGTCCCT GGTGCCCTAC AGCAAGCCCA GCTTCCCGTC TCCTGGCGGG 
CACAGCTCAT CAGGAACATC TTCCTCTAAA GGGTCCACTG GCCCTCGGAA GGCTGACGTG 
CTGAGAGGCA GCCACCAGAG GAATGCCAAT GACCTTCTCG ACATTGGATA TGTGGGCTCA 
AATAGTCAAG GACAGTTTAC AGGTGAATTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523101.1
CDS	245..5017
Translation	MARRQESICGRPWTWTPGLLMLIILGIHQGSGQGQGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKAMSTDEGTYVCIAENRVGKVEASATLTVRVRPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPINQTLAVDGTALLKCKATGEPLPVISWLKEGFTFLGRDPRATIQDQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDMNDLPGPPSKPQVTDVSKNSVTLSWQPGTPGVLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVVVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQASTVWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSHNSTSISVSWDPPPADHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVVIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGGRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPNYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTTYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSVQQKTDLMGFGYSLPDQNKGNNALLYIPDYRLAEGLSNRMPHNQSQDFSTTSSHNSSERSGSLSGGKGGKKKKTKNSSKAQKNNGSTWANVPLPPPPVQPLPGTELGHYAAEQENGYDSDSWCPPLPVQTYLHQGMEDELEEDEDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNTPPPQPPAPPLGYVSGALISDLETDVPDEDADDEEEPLEIPRPLRALDQTPGSSMDNLDSSVTGSMVNGWGSASDEDRNFSSHRSSVGSSSDGSIFASGSFAKALVAAADKAGFRLDGTSLTRTGKAFSSSQRQRPTSPFSTDSNTSAAQNQSQRPRPTKKHKGGRMDPQPVLPHRREGMPDDLPPPPDPPPGQGLRQQIGLSQHSGNVENSTERKGSSLERQQAANLEDTKSSLDCPAKTVLEWQRQTQDWINSTERQEETRKAPHKQGVGSEESLVPYSKPSFPSPGGHSSSGTSSSKGSTGPRKADVLRGSHQRNANDLLDIGYVGSNSQGQFTGEL

Target ORF information:

RefSeq Version	XM_006523038.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523038.3

ORF Insert Sequence:

ATGGCCAGAA GACAAGAAAG TATCTGTGGA AGGCCGTGGA CGTGGACGCC AGGACTACTG 
ATGCTGATCA TCCTTGGCAT TCATCAGGGG AGTGGGCAAG GTCAAGGATC TCGTCTTCGT 
CAAGAAGACT TTCCCCCCAG GATCGTAGAA CACCCTTCTG ATGTCATCGT CTCCAAGGGA 
GAGCCCACCA CTCTGAACTG TAAAGCAGAG GGCCGACCCA CCCCCACCAT TGAATGGTAC 
AAGGATGGTG AGAGGGTGGA GACAGACAAG GATGATCCCA GGTCCCACAG AATGCTTCTG 
CCCAGCGGAT CTTTATTCTT TTTGCGAATT GTTCATGGGC GCAGAAGTAA ACCGGACGAA 
GGGAGTTACG TTTGTGTTGC AAGGAACTAT CTTGGTGAAG CAGTGAGTCG AAATGCATCT 
CTGGAAGTGG CATTATTGCG AGATGACTTC CGGCAAAACC CCACAGATGT GGTAGTCGCA 
GCTGGAGAGC CTGCAATCTT GGAGTGCCAG CCACCACGGG GACACCCAGA ACCAACCATC 
TACTGGAAAA AGGACAAAGT CCGAATTGAT GACAAGGAAG AGAGAATAAG TATCCGTGGT 
GGGAAGCTGA TGATCTCTAA TACTAGGAAA AGCGATGCTG GCATGTACAC CTGTGTGGGA 
ACCAATATGG TGGGAGAAAG GGACAGCGAC CCTGCAGAGC TCACTGTCTT TGAACGACCC 
ACATTTCTCA GGAGGCCAAT TAACCAGGTG GTGCTAGAGG AAGAAGCTGT AGAATTCCGT 
TGTCAGGTCC AAGGAGATCC CCAGCCAACG GTGAGGTGGA AAAAAGATGA TGCAGACTTG 
CCGAGAGGAA GGTATGATAT CAAAGATGAC TACACGCTGA GAATTAAAAA GGCCATGAGT 
ACTGATGAAG GTACCTATGT GTGTATTGCT GAGAATCGGG TGGGAAAAGT GGAAGCCTCT 
GCTACCCTCA CTGTCCGAGT TCGCCCTGTT GCTCCTCCAC AGTTTGTGGT TAGGCCAAGA 
GATCAGATCG TTGCTCAAGG CCGAACAGTG ACATTCCCCT GTGAAACTAA AGGAAACCCA 
CAGCCAGCTG TTTTTTGGCA GAAAGAAGGC AGCCAGAACC TACTTTTCCC GAATCAACCT 
CAGCAGCCCA ACAGCCGATG TTCAGTGTCG CCCACGGGGG ACCTCACCAT CACCAACATC 
CAGCGTTCAG ATGCGGGTTA CTACATCTGC CAGGCCCTAA CCGTGGCAGG AAGCATTTTA 
GCTAAAGCAC AGTTGGAAGT TACTGACGTT TTGACAGATA GACCTCCACC CATAATCTTG 
CAAGGACCAA TAAACCAAAC ACTTGCAGTA GACGGTACAG CATTGTTGAA GTGTAAAGCC 
ACTGGTGAGC CTCTGCCTGT AATTAGCTGG CTAAAGGAGG GCTTTACTTT TCTGGGGAGA 
GATCCAAGAG CCACGATCCA AGACCAAGGA ACACTGCAGA TTAAGAATTT ACGGATATCT 
GATACTGGCA CTTATACTTG TGTGGCTACA AGTTCCAGTG GAGAGACTTC CTGGAGTGCA 
GTGCTGGATG TAACAGAATC TGGAGCAACA ATCAGTAAAA ATTATGATAT GAATGACCTC 
CCGGGACCAC CATCCAAACC TCAGGTCACT GATGTTTCTA AGAACAGTGT CACCTTATCC 
TGGCAGCCAG GTACACCTGG CGTTCTTCCT GCAAGCGCGT ATATCATTGA GGCTTTCAGC 
CAATCGGTGA GCAATAGCTG GCAGACAGTG GCAAACCATG TTAAGACAAC TCTGTATACA 
GTAAGGGGGC TGAGGCCCAA CACAATCTAC TTGTTTATGG TCAGAGCGAT CAACCCACAA 
GGTCTCAGTG ATCCAAGTCC TATGTCGGAT CCTGTACGCA CACAAGATAT CAGCCCCCCA 
GCACAAGGAG TGGACCACAG ACAGGTGCAG AAGGAATTAG GTGATGTCGT TGTTCGTCTC 
CATAATCCAG TTGTCCTGAC ACCTACAACT GTTCAAGTCA CATGGACGGT GGACCGACAA 
CCCCAGTTTA TTCAGGGCTA CAGAGTGATG TACCGTCAGA CTTCGGGACT ACAAGCCTCA 
ACTGTGTGGC AGAATCTAGA CGCCAAAGTC CCGACTGAGA GGAGTGCTGT CCTTGTGAAT 
TTGAAAAAGG GGGTGACTTA TGAAATTAAA GTCCGGCCGT ATTTTAACGA GTTCCAAGGA 
ATGGACAGTG AATCGAAAAC AGTCCGAACC ACTGAGGAAG CCCCAAGTGC CCCTCCCCAG 
TCTGTCACTG TGCTGACAGT TGGAAGTCAC AACAGCACAA GCATCAGTGT TTCCTGGGAT 
CCTCCACCAG CCGACCACCA GAATGGAATT ATTCAGGAAT ATAAGATCTG GTGTCTGGGA 
AACGAAACGC GATTCCATAT CAATAAAACG GTGGATGCAG CCATTCGCTC TGTAGTAATA 
GGTGGCTTGT TCCCTGGAAT TCAGTACCGG GTAGAAGTGG CAGCTAGCAC AAGTGCAGGG 
GTTGGAGTAA AAAGTGAACC ACAGCCGATA ATAATTGGGG GACGTAACGA AGTTGTCATT 
ACTGAAAACA ATAACAGCAT CACTGAGCAA ATCACGGATG TCGTGAAGCA ACCGGCATTT 
ATAGCTGGCA TTGGTGGTGC CTGCTGGGTA ATTCTGATGG GTTTTAGCAT CTGGTTGTAC 
TGGAGAAGAA AGAAGAGAAA GGGACTCAGC AATTATGCTG TAACATTTCA AAGAGGAGAT 
GGAGGACTAA TGAGCAATGG GAGCCGTCCA GGTCTTCTAA ATGCTGGCGA TCCCAATTAC 
CCATGGCTTG CTGATTCTTG GCCAGCCACG AGTTTGCCAG TGAACAATAG CAATAGTGGC 
CCAAATGAAA TTGGAAATTT TGGGCGTGGA GATGTGCTGC CTCCGGTGCC AGGCCAAGGG 
GATAAAACAG CGACCATGCT CTCGGATGGA GCCATTTATA GCAGCATTGA CTTCACTACC 
AAAACCACTT ACAACAGTTC CAGCCAAATA ACACAGGCCA CCCCATATGC CACTACACAA 
ATCCTGCATT CAAACAGCAT CCACGAACTG GCAGTTGATC TTCCTGATCC ACAGTGGAAA 
AGCTCAGTTC AACAGAAGAC AGACCTCATG GGATTTGGTT ATTCGCTACC TGATCAGAAC 
AAGGGGAACA ACGCCTTACT TTACATCCCT GACTACCGAT TGGCTGAGGG ATTGTCTAAT 
AGAATGCCAC ACAACCAGTC ACAGGATTTC AGCACCACCA GCTCTCACAA CAGCTCAGAA 
AGGAGTGGCA GTCTCTCAGG TGGGAAAGGT GGAAAAAAGA AGAAAACTAA AAATTCTTCG 
AAAGCGCAGA AAAACAACGG ATCCACTTGG GCTAATGTCC CTCTACCTCC TCCTCCCGTC 
CAGCCCCTTC CTGGTACAGA GCTGGGCCAC TATGCTGCGG AACAAGAAAA TGGCTATGAC 
AGTGACAGCT GGTGCCCACC ATTACCGGTG CAAACATACT TGCATCAGGG TATGGAAGAT 
GAGCTGGAAG AAGACGAAGA TCGGGTCCCA ACACCTCCTG TTCGCGGCGT GGCCTCTTCA 
CCAGCTATCT CTTTTGGACA GCAGTCCACT GCCACTCTTA CTCCATCCCC ACGGGAAGAG 
ATGCAACCCA TGCTGCAAGC TCACTTGGAT GAGTTGACAA GGGCCTATCA GTTTGATATA 
GCAAAACAAA CATGGCACAT TCAAAGCAAT ACCCCACCTC CACAACCCCC AGCTCCGCCA 
TTAGGTTACG TGTCCGGAGC CTTGATTTCT GATTTGGAGA CAGATGTTCC AGATGAGGAT 
GCTGATGATG AAGAGGAACC ATTAGAAATT CCCAGGCCCC TCAGAGCACT AGACCAGACA 
CCTGGATCCA GTATGGACAA TCTAGACAGC TCTGTCACAG GTTCAATGGT AAATGGATGG 
GGCTCTGCAT CTGATGAGGA TCGTAACTTT TCTAGTCATA GATCTAGTGT AGGTAGCTCC 
TCAGATGGCT CCATCTTTGC CAGCGGCAGC TTTGCAAAAG CATTGGTGGC AGCAGCAGAC 
AAAGCTGGCT TTAGGCTGGA TGGAACCAGT CTTACGAGAA CAGGAAAAGC CTTTAGCTCC 
TCTCAAAGGC AGCGGCCCAC CAGCCCATTT TCTACGGACA GTAACACCAG TGCTGCCCAG 
AATCAAAGCC AGAGGCCTCG GCCCACGAAA AAACATAAGG GAGGACGGAT GGACCCACAG 
CCAGTGTTAC CTCATCGGAG GGAAGGGATG CCAGATGATC TTCCCCCACC ACCAGATCCC 
CCACCAGGTC AGGGTTTAAG GCAGCAGATA GGCCTGAGCC AACACTCTGG TAACGTGGAA 
AACTCAACAG AGAGAAAAGG AAGCTCTCTA GAGAGACAAC AAGCAGCCAA CTTAGAAGAC 
ACAAAGAGCT CATTGGATTG TCCAGCTAAA ACTGTTCTAG AGTGGCAGCG TCAAACCCAG 
GACTGGATAA ACTCTACAGA ACGCCAAGAA GAAACTCGGA AAGCCCCACA CAAACAAGGG 
GTTGGATCAG AGGAGTCCCT GGTGCCCTAC AGCAAGCCCA GCTTCCCGTC TCCTGGCGGG 
CACAGCTCAT CAGGAACATC TTCCTCTAAA GGGTCCACTG GCCCTCGGAA GGCTGACGTG 
CTGAGAGGCA GCCACCAGAG GAATGCCAAT GACCTTCTCG ACATTGGATA TGTGGGCTCA 
AATAGTCAAG GACAGTTTAC AGGTGAATTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo2 ( XM_006523039.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523102.1 Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X5, mRNA.

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CloneID	OMu52759
Clone ID Related Accession (Same CDS sequence)	XM_006523039.4 , XM_006523039.3
Accession Version	XM_006523039.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4728bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	roundabout homolog 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006523039.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCCTC TGATGTTTAC ACTATTATTG CTCTTTGGAT TTCTCTGCAT TCAGATTGAT 
GGATCTCGTC TTCGTCAAGA AGACTTTCCC CCCAGGATCG TAGAACACCC TTCTGATGTC 
ATCGTCTCCA AGGGAGAGCC CACCACTCTG AACTGTAAAG CAGAGGGCCG ACCCACCCCC 
ACCATTGAAT GGTACAAGGA TGGTGAGAGG GTGGAGACAG ACAAGGATGA TCCCAGGTCC 
CACAGAATGC TTCTGCCCAG CGGATCTTTA TTCTTTTTGC GAATTGTTCA TGGGCGCAGA 
AGTAAACCGG ACGAAGGGAG TTACGTTTGT GTTGCAAGGA ACTATCTTGG TGAAGCAGTG 
AGTCGAAATG CATCTCTGGA AGTGGCATTA TTGCGAGATG ACTTCCGGCA AAACCCCACA 
GATGTGGTAG TCGCAGCTGG AGAGCCTGCA ATCTTGGAGT GCCAGCCACC ACGGGGACAC 
CCAGAACCAA CCATCTACTG GAAAAAGGAC AAAGTCCGAA TTGATGACAA GGAAGAGAGA 
ATAAGTATCC GTGGTGGGAA GCTGATGATC TCTAATACTA GGAAAAGCGA TGCTGGCATG 
TACACCTGTG TGGGAACCAA TATGGTGGGA GAAAGGGACA GCGACCCTGC AGAGCTCACT 
GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTGCT AGAGGAAGAA 
GCTGTAGAAT TCCGTTGTCA GGTCCAAGGA GATCCCCAGC CAACGGTGAG GTGGAAAAAA 
GATGATGCAG ACTTGCCGAG AGGAAGGTAT GATATCAAAG ATGACTACAC GCTGAGAATT 
AAAAAGGCCA TGAGTACTGA TGAAGGTACC TATGTGTGTA TTGCTGAGAA TCGGGTGGGA 
AAAGTGGAAG CCTCTGCTAC CCTCACTGTC CGAGTTCGCC CTGTTGCTCC TCCACAGTTT 
GTGGTTAGGC CAAGAGATCA GATCGTTGCT CAAGGCCGAA CAGTGACATT CCCCTGTGAA 
ACTAAAGGAA ACCCACAGCC AGCTGTTTTT TGGCAGAAAG AAGGCAGCCA GAACCTACTT 
TTCCCGAATC AACCTCAGCA GCCCAACAGC CGATGTTCAG TGTCGCCCAC GGGGGACCTC 
ACCATCACCA ACATCCAGCG TTCAGATGCG GGTTACTACA TCTGCCAGGC CCTAACCGTG 
GCAGGAAGCA TTTTAGCTAA AGCACAGTTG GAAGTTACTG ACGTTTTGAC AGATAGACCT 
CCACCCATAA TCTTGCAAGG ACCAATAAAC CAAACACTTG CAGTAGACGG TACAGCATTG 
TTGAAGTGTA AAGCCACTGG TGAGCCTCTG CCTGTAATTA GCTGGCTAAA GGAGGGCTTT 
ACTTTTCTGG GGAGAGATCC AAGAGCCACG ATCCAAGACC AAGGAACACT GCAGATTAAG 
AATTTACGGA TATCTGATAC TGGCACTTAT ACTTGTGTGG CTACAAGTTC CAGTGGAGAG 
ACTTCCTGGA GTGCAGTGCT GGATGTAACA GAATCTGGAG CAACAATCAG TAAAAATTAT 
GATATGAATG ACCTCCCGGG ACCACCATCC AAACCTCAGG TCACTGATGT TTCTAAGAAC 
AGTGTCACCT TATCCTGGCA GCCAGGTACA CCTGGCGTTC TTCCTGCAAG CGCGTATATC 
ATTGAGGCTT TCAGCCAATC GGTGAGCAAT AGCTGGCAGA CAGTGGCAAA CCATGTTAAG 
ACAACTCTGT ATACAGTAAG GGGGCTGAGG CCCAACACAA TCTACTTGTT TATGGTCAGA 
GCGATCAACC CACAAGGTCT CAGTGATCCA AGTCCTATGT CGGATCCTGT ACGCACACAA 
GATATCAGCC CCCCAGCACA AGGAGTGGAC CACAGACAGG TGCAGAAGGA ATTAGGTGAT 
GTCGTTGTTC GTCTCCATAA TCCAGTTGTC CTGACACCTA CAACTGTTCA AGTCACATGG 
ACGGTGGACC GACAACCCCA GTTTATTCAG GGCTACAGAG TGATGTACCG TCAGACTTCG 
GGACTACAAG CCTCAACTGT GTGGCAGAAT CTAGACGCCA AAGTCCCGAC TGAGAGGAGT 
GCTGTCCTTG TGAATTTGAA AAAGGGGGTG ACTTATGAAA TTAAAGTCCG GCCGTATTTT 
AACGAGTTCC AAGGAATGGA CAGTGAATCG AAAACAGTCC GAACCACTGA GGAAGCCCCA 
AGTGCCCCTC CCCAGTCTGT CACTGTGCTG ACAGTTGGAA GTCACAACAG CACAAGCATC 
AGTGTTTCCT GGGATCCTCC ACCAGCCGAC CACCAGAATG GAATTATTCA GGAATATAAG 
ATCTGGTGTC TGGGAAACGA AACGCGATTC CATATCAATA AAACGGTGGA TGCAGCCATT 
CGCTCTGTAG TAATAGGTGG CTTGTTCCCT GGAATTCAGT ACCGGGTAGA AGTGGCAGCT 
AGCACAAGTG CAGGGGTTGG AGTAAAAAGT GAACCACAGC CGATAATAAT TGGGGGACGT 
AACGAAGTTG TCATTACTGA AAACAATAAC AGCATCACTG AGCAAATCAC GGATGTCGTG 
AAGCAACCGG CATTTATAGC TGGCATTGGT GGTGCCTGCT GGGTAATTCT GATGGGTTTT 
AGCATCTGGT TGTACTGGAG AAGAAAGAAG AGAAAGGGAC TCAGCAATTA TGCTGTAACA 
TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGGAGCC GTCCAGGTCT TCTAAATGCT 
GGCGATCCCA ATTACCCATG GCTTGCTGAT TCTTGGCCAG CCACGAGTTT GCCAGTGAAC 
AATAGCAATA GTGGCCCAAA TGAAATTGGA AATTTTGGGC GTGGAGATGT GCTGCCTCCG 
GTGCCAGGCC AAGGGGATAA AACAGCGACC ATGCTCTCGG ATGGAGCCAT TTATAGCAGC 
ATTGACTTCA CTACCAAAAC CACTTACAAC AGTTCCAGCC AAATAACACA GGCCACCCCA 
TATGCCACTA CACAAATCCT GCATTCAAAC AGCATCCACG AACTGGCAGT TGATCTTCCT 
GATCCACAGT GGAAAAGCTC AGTTCAACAG AAGACAGACC TCATGGGATT TGGTTATTCG 
CTACCTGATC AGAACAAGGG GAACAACGCC TTACTTTACA TCCCTGACTA CCGATTGGCT 
GAGGGATTGT CTAATAGAAT GCCACACAAC CAGTCACAGG ATTTCAGCAC CACCAGCTCT 
CACAACAGCT CAGAAAGGAG TGGCAGTCTC TCAGGTGGGA AAGGTGGAAA AAAGAAGAAA 
ACTAAAAATT CTTCGAAAGC GCAGAAAAAC AACGGATCCA CTTGGGCTAA TGTCCCTCTA 
CCTCCTCCTC CCGTCCAGCC CCTTCCTGGT ACAGAGCTGG GCCACTATGC TGCGGAACAA 
GAAAATGGCT ATGACAGTGA CAGCTGGTGC CCACCATTAC CGGTGCAAAC ATACTTGCAT 
CAGGGTATGG AAGATGAGCT GGAAGAAGAC GAAGATCGGG TCCCAACACC TCCTGTTCGC 
GGCGTGGCCT CTTCACCAGC TATCTCTTTT GGACAGCAGT CCACTGCCAC TCTTACTCCA 
TCCCCACGGG AAGAGATGCA ACCCATGCTG CAAGCTCACT TGGATGAGTT GACAAGGGCC 
TATCAGTTTG ATATAGCAAA ACAAACATGG CACATTCAAA GCAATACCCC ACCTCCACAA 
CCCCCAGCTC CGCCATTAGG TTACGTGTCC GGAGCCTTGA TTTCTGATTT GGAGACAGAT 
GTTCCAGATG AGGATGCTGA TGATGAAGAG GAACCATTAG AAATTCCCAG GCCCCTCAGA 
GCACTAGACC AGACACCTGG ATCCAGTATG GACAATCTAG ACAGCTCTGT CACAGGTTCA 
ATGGTAAATG GATGGGGCTC TGCATCTGAT GAGGATCGTA ACTTTTCTAG TCATAGATCT 
AGTGTAGGTA GCTCCTCAGA TGGCTCCATC TTTGCCAGCG GCAGCTTTGC AAAAGCATTG 
GTGGCAGCAG CAGACAAAGC TGGCTTTAGG CTGGATGGAA CCAGTCTTAC GAGAACAGGA 
AAAGCCTTTA GCTCCTCTCA AAGGCAGCGG CCCACCAGCC CATTTTCTAC GGACAGTAAC 
ACCAGTGCTG CCCAGAATCA AAGCCAGAGG CCTCGGCCCA CGAAAAAACA TAAGGGAGGA 
CGGATGGACC CACAGCCAGT GTTACCTCAT CGGAGGGAAG GGATGCCAGA TGATCTTCCC 
CCACCACCAG ATCCCCCACC AGGTCAGGGT TTAAGGCAGC AGATAGGCCT GAGCCAACAC 
TCTGGTAACG TGGAAAACTC AACAGAGAGA AAAGGAAGCT CTCTAGAGAG ACAACAAGCA 
GCCAACTTAG AAGACACAAA GAGCTCATTG GATTGTCCAG CTAAAACTGT TCTAGAGTGG 
CAGCGTCAAA CCCAGGACTG GATAAACTCT ACAGAACGCC AAGAAGAAAC TCGGAAAGCC 
CCACACAAAC AAGGGGTTGG ATCAGAGGAG TCCCTGGTGC CCTACAGCAA GCCCAGCTTC 
CCGTCTCCTG GCGGGCACAG CTCATCAGGA ACATCTTCCT CTAAAGGGTC CACTGGCCCT 
CGGAAGGCTG ACGTGCTGAG AGGCAGCCAC CAGAGGAATG CCAATGACCT TCTCGACATT 
GGATATGTGG GCTCAAATAG TCAAGGACAG TTTACAGGTG AATTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523102.1
CDS	2415..7142
Translation	MNPLMFTLLLLFGFLCIQIDGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKAMSTDEGTYVCIAENRVGKVEASATLTVRVRPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPINQTLAVDGTALLKCKATGEPLPVISWLKEGFTFLGRDPRATIQDQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDMNDLPGPPSKPQVTDVSKNSVTLSWQPGTPGVLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVVVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQASTVWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSHNSTSISVSWDPPPADHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVVIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGGRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPNYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTTYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSVQQKTDLMGFGYSLPDQNKGNNALLYIPDYRLAEGLSNRMPHNQSQDFSTTSSHNSSERSGSLSGGKGGKKKKTKNSSKAQKNNGSTWANVPLPPPPVQPLPGTELGHYAAEQENGYDSDSWCPPLPVQTYLHQGMEDELEEDEDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNTPPPQPPAPPLGYVSGALISDLETDVPDEDADDEEEPLEIPRPLRALDQTPGSSMDNLDSSVTGSMVNGWGSASDEDRNFSSHRSSVGSSSDGSIFASGSFAKALVAAADKAGFRLDGTSLTRTGKAFSSSQRQRPTSPFSTDSNTSAAQNQSQRPRPTKKHKGGRMDPQPVLPHRREGMPDDLPPPPDPPPGQGLRQQIGLSQHSGNVENSTERKGSSLERQQAANLEDTKSSLDCPAKTVLEWQRQTQDWINSTERQEETRKAPHKQGVGSEESLVPYSKPSFPSPGGHSSSGTSSSKGSTGPRKADVLRGSHQRNANDLLDIGYVGSNSQGQFTGEL

Target ORF information:

RefSeq Version	XM_006523039.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523039.3

ORF Insert Sequence:

ATGAATCCTC TGATGTTTAC ACTATTATTG CTCTTTGGAT TTCTCTGCAT TCAGATTGAT 
GGATCTCGTC TTCGTCAAGA AGACTTTCCC CCCAGGATCG TAGAACACCC TTCTGATGTC 
ATCGTCTCCA AGGGAGAGCC CACCACTCTG AACTGTAAAG CAGAGGGCCG ACCCACCCCC 
ACCATTGAAT GGTACAAGGA TGGTGAGAGG GTGGAGACAG ACAAGGATGA TCCCAGGTCC 
CACAGAATGC TTCTGCCCAG CGGATCTTTA TTCTTTTTGC GAATTGTTCA TGGGCGCAGA 
AGTAAACCGG ACGAAGGGAG TTACGTTTGT GTTGCAAGGA ACTATCTTGG TGAAGCAGTG 
AGTCGAAATG CATCTCTGGA AGTGGCATTA TTGCGAGATG ACTTCCGGCA AAACCCCACA 
GATGTGGTAG TCGCAGCTGG AGAGCCTGCA ATCTTGGAGT GCCAGCCACC ACGGGGACAC 
CCAGAACCAA CCATCTACTG GAAAAAGGAC AAAGTCCGAA TTGATGACAA GGAAGAGAGA 
ATAAGTATCC GTGGTGGGAA GCTGATGATC TCTAATACTA GGAAAAGCGA TGCTGGCATG 
TACACCTGTG TGGGAACCAA TATGGTGGGA GAAAGGGACA GCGACCCTGC AGAGCTCACT 
GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTGCT AGAGGAAGAA 
GCTGTAGAAT TCCGTTGTCA GGTCCAAGGA GATCCCCAGC CAACGGTGAG GTGGAAAAAA 
GATGATGCAG ACTTGCCGAG AGGAAGGTAT GATATCAAAG ATGACTACAC GCTGAGAATT 
AAAAAGGCCA TGAGTACTGA TGAAGGTACC TATGTGTGTA TTGCTGAGAA TCGGGTGGGA 
AAAGTGGAAG CCTCTGCTAC CCTCACTGTC CGAGTTCGCC CTGTTGCTCC TCCACAGTTT 
GTGGTTAGGC CAAGAGATCA GATCGTTGCT CAAGGCCGAA CAGTGACATT CCCCTGTGAA 
ACTAAAGGAA ACCCACAGCC AGCTGTTTTT TGGCAGAAAG AAGGCAGCCA GAACCTACTT 
TTCCCGAATC AACCTCAGCA GCCCAACAGC CGATGTTCAG TGTCGCCCAC GGGGGACCTC 
ACCATCACCA ACATCCAGCG TTCAGATGCG GGTTACTACA TCTGCCAGGC CCTAACCGTG 
GCAGGAAGCA TTTTAGCTAA AGCACAGTTG GAAGTTACTG ACGTTTTGAC AGATAGACCT 
CCACCCATAA TCTTGCAAGG ACCAATAAAC CAAACACTTG CAGTAGACGG TACAGCATTG 
TTGAAGTGTA AAGCCACTGG TGAGCCTCTG CCTGTAATTA GCTGGCTAAA GGAGGGCTTT 
ACTTTTCTGG GGAGAGATCC AAGAGCCACG ATCCAAGACC AAGGAACACT GCAGATTAAG 
AATTTACGGA TATCTGATAC TGGCACTTAT ACTTGTGTGG CTACAAGTTC CAGTGGAGAG 
ACTTCCTGGA GTGCAGTGCT GGATGTAACA GAATCTGGAG CAACAATCAG TAAAAATTAT 
GATATGAATG ACCTCCCGGG ACCACCATCC AAACCTCAGG TCACTGATGT TTCTAAGAAC 
AGTGTCACCT TATCCTGGCA GCCAGGTACA CCTGGCGTTC TTCCTGCAAG CGCGTATATC 
ATTGAGGCTT TCAGCCAATC GGTGAGCAAT AGCTGGCAGA CAGTGGCAAA CCATGTTAAG 
ACAACTCTGT ATACAGTAAG GGGGCTGAGG CCCAACACAA TCTACTTGTT TATGGTCAGA 
GCGATCAACC CACAAGGTCT CAGTGATCCA AGTCCTATGT CGGATCCTGT ACGCACACAA 
GATATCAGCC CCCCAGCACA AGGAGTGGAC CACAGACAGG TGCAGAAGGA ATTAGGTGAT 
GTCGTTGTTC GTCTCCATAA TCCAGTTGTC CTGACACCTA CAACTGTTCA AGTCACATGG 
ACGGTGGACC GACAACCCCA GTTTATTCAG GGCTACAGAG TGATGTACCG TCAGACTTCG 
GGACTACAAG CCTCAACTGT GTGGCAGAAT CTAGACGCCA AAGTCCCGAC TGAGAGGAGT 
GCTGTCCTTG TGAATTTGAA AAAGGGGGTG ACTTATGAAA TTAAAGTCCG GCCGTATTTT 
AACGAGTTCC AAGGAATGGA CAGTGAATCG AAAACAGTCC GAACCACTGA GGAAGCCCCA 
AGTGCCCCTC CCCAGTCTGT CACTGTGCTG ACAGTTGGAA GTCACAACAG CACAAGCATC 
AGTGTTTCCT GGGATCCTCC ACCAGCCGAC CACCAGAATG GAATTATTCA GGAATATAAG 
ATCTGGTGTC TGGGAAACGA AACGCGATTC CATATCAATA AAACGGTGGA TGCAGCCATT 
CGCTCTGTAG TAATAGGTGG CTTGTTCCCT GGAATTCAGT ACCGGGTAGA AGTGGCAGCT 
AGCACAAGTG CAGGGGTTGG AGTAAAAAGT GAACCACAGC CGATAATAAT TGGGGGACGT 
AACGAAGTTG TCATTACTGA AAACAATAAC AGCATCACTG AGCAAATCAC GGATGTCGTG 
AAGCAACCGG CATTTATAGC TGGCATTGGT GGTGCCTGCT GGGTAATTCT GATGGGTTTT 
AGCATCTGGT TGTACTGGAG AAGAAAGAAG AGAAAGGGAC TCAGCAATTA TGCTGTAACA 
TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGGAGCC GTCCAGGTCT TCTAAATGCT 
GGCGATCCCA ATTACCCATG GCTTGCTGAT TCTTGGCCAG CCACGAGTTT GCCAGTGAAC 
AATAGCAATA GTGGCCCAAA TGAAATTGGA AATTTTGGGC GTGGAGATGT GCTGCCTCCG 
GTGCCAGGCC AAGGGGATAA AACAGCGACC ATGCTCTCGG ATGGAGCCAT TTATAGCAGC 
ATTGACTTCA CTACCAAAAC CACTTACAAC AGTTCCAGCC AAATAACACA GGCCACCCCA 
TATGCCACTA CACAAATCCT GCATTCAAAC AGCATCCACG AACTGGCAGT TGATCTTCCT 
GATCCACAGT GGAAAAGCTC AGTTCAACAG AAGACAGACC TCATGGGATT TGGTTATTCG 
CTACCTGATC AGAACAAGGG GAACAACGCC TTACTTTACA TCCCTGACTA CCGATTGGCT 
GAGGGATTGT CTAATAGAAT GCCACACAAC CAGTCACAGG ATTTCAGCAC CACCAGCTCT 
CACAACAGCT CAGAAAGGAG TGGCAGTCTC TCAGGTGGGA AAGGTGGAAA AAAGAAGAAA 
ACTAAAAATT CTTCGAAAGC GCAGAAAAAC AACGGATCCA CTTGGGCTAA TGTCCCTCTA 
CCTCCTCCTC CCGTCCAGCC CCTTCCTGGT ACAGAGCTGG GCCACTATGC TGCGGAACAA 
GAAAATGGCT ATGACAGTGA CAGCTGGTGC CCACCATTAC CGGTGCAAAC ATACTTGCAT 
CAGGGTATGG AAGATGAGCT GGAAGAAGAC GAAGATCGGG TCCCAACACC TCCTGTTCGC 
GGCGTGGCCT CTTCACCAGC TATCTCTTTT GGACAGCAGT CCACTGCCAC TCTTACTCCA 
TCCCCACGGG AAGAGATGCA ACCCATGCTG CAAGCTCACT TGGATGAGTT GACAAGGGCC 
TATCAGTTTG ATATAGCAAA ACAAACATGG CACATTCAAA GCAATACCCC ACCTCCACAA 
CCCCCAGCTC CGCCATTAGG TTACGTGTCC GGAGCCTTGA TTTCTGATTT GGAGACAGAT 
GTTCCAGATG AGGATGCTGA TGATGAAGAG GAACCATTAG AAATTCCCAG GCCCCTCAGA 
GCACTAGACC AGACACCTGG ATCCAGTATG GACAATCTAG ACAGCTCTGT CACAGGTTCA 
ATGGTAAATG GATGGGGCTC TGCATCTGAT GAGGATCGTA ACTTTTCTAG TCATAGATCT 
AGTGTAGGTA GCTCCTCAGA TGGCTCCATC TTTGCCAGCG GCAGCTTTGC AAAAGCATTG 
GTGGCAGCAG CAGACAAAGC TGGCTTTAGG CTGGATGGAA CCAGTCTTAC GAGAACAGGA 
AAAGCCTTTA GCTCCTCTCA AAGGCAGCGG CCCACCAGCC CATTTTCTAC GGACAGTAAC 
ACCAGTGCTG CCCAGAATCA AAGCCAGAGG CCTCGGCCCA CGAAAAAACA TAAGGGAGGA 
CGGATGGACC CACAGCCAGT GTTACCTCAT CGGAGGGAAG GGATGCCAGA TGATCTTCCC 
CCACCACCAG ATCCCCCACC AGGTCAGGGT TTAAGGCAGC AGATAGGCCT GAGCCAACAC 
TCTGGTAACG TGGAAAACTC AACAGAGAGA AAAGGAAGCT CTCTAGAGAG ACAACAAGCA 
GCCAACTTAG AAGACACAAA GAGCTCATTG GATTGTCCAG CTAAAACTGT TCTAGAGTGG 
CAGCGTCAAA CCCAGGACTG GATAAACTCT ACAGAACGCC AAGAAGAAAC TCGGAAAGCC 
CCACACAAAC AAGGGGTTGG ATCAGAGGAG TCCCTGGTGC CCTACAGCAA GCCCAGCTTC 
CCGTCTCCTG GCGGGCACAG CTCATCAGGA ACATCTTCCT CTAAAGGGTC CACTGGCCCT 
CGGAAGGCTG ACGTGCTGAG AGGCAGCCAC CAGAGGAATG CCAATGACCT TCTCGACATT 
GGATATGTGG GCTCAAATAG TCAAGGACAG TTTACAGGTG AATTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo2 ( XM_006523040.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523103.1 Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X6, mRNA.

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CloneID	OMu52760
Clone ID Related Accession (Same CDS sequence)	XM_006523040.4 , XM_006523040.3
Accession Version	XM_006523040.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4671bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	roundabout homolog 2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006523040.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT 
CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG 
GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC 
CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA 
GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT 
ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG 
GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG 
CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT 
AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT 
TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC 
GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC 
CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT 
ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA 
CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA 
TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA 
GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT 
CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT 
GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG 
CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG 
GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC 
ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG 
GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT 
GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA 
GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT 
GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG 
GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT 
GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT 
CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG 
CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG 
CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT 
TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG 
GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT 
GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT 
GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGGTG GGAAAGGTGG AAAAAAGAAG 
AAAACTAAAA ATTCTTCGAA AGCGCAGAAA AACAACGGAT CCACTTGGGC TAATGTCCCT 
CTACCTCCTC CTCCCGTCCA GCCCCTTCCT GGTACAGAGC TGGGCCACTA TGCTGCGGAA 
CAAGAAAATG GCTATGACAG TGACAGCTGG TGCCCACCAT TACCGGTGCA AACATACTTG 
CATCAGGGTA TGGAAGATGA GCTGGAAGAA GACGAAGATC GGGTCCCAAC ACCTCCTGTT 
CGCGGCGTGG CCTCTTCACC AGCTATCTCT TTTGGACAGC AGTCCACTGC CACTCTTACT 
CCATCCCCAC GGGAAGAGAT GCAACCCATG CTGCAAGCTC ACTTGGATGA GTTGACAAGG 
GCCTATCAGT TTGATATAGC AAAACAAACA TGGCACATTC AAAGCAATAC CCCACCTCCA 
CAACCCCCAG CTCCGCCATT AGGTTACGTG TCCGGAGCCT TGATTTCTGA TTTGGAGACA 
GATGTTCCAG ATGAGGATGC TGATGATGAA GAGGAACCAT TAGAAATTCC CAGGCCCCTC 
AGAGCACTAG ACCAGACACC TGGATCCAGT ATGGACAATC TAGACAGCTC TGTCACAGGT 
TCAATGGTAA ATGGATGGGG CTCTGCATCT GATGAGGATC GTAACTTTTC TAGTCATAGA 
TCTAGTGTAG GTAGCTCCTC AGATGGCTCC ATCTTTGCCA GCGGCAGCTT TGCAAAAGCA 
TTGGTGGCAG CAGCAGACAA AGCTGGCTTT AGGCTGGATG GAACCAGTCT TACGAGAACA 
GGAAAAGCCT TTAGCTCCTC TCAAAGGCAG CGGCCCACCA GCCCATTTTC TACGGACAGT 
AACACCAGTG CTGCCCAGAA TCAAAGCCAG AGGCCTCGGC CCACGAAAAA ACATAAGGGA 
GGACGGATGG ACCCACAGCC AGTGTTACCT CATCGGAGGG AAGGGATGCC AGATGATCTT 
CCCCCACCAC CAGATCCCCC ACCAGGTCAG GGTTTAAGGC AGCAGATAGG CCTGAGCCAA 
CACTCTGGTA ACGTGGAAAA CTCAACAGAG AGAAAAGGAA GCTCTCTAGA GAGACAACAA 
GCAGCCAACT TAGAAGACAC AAAGAGCTCA TTGGATTGTC CAGCTAAAAC TGTTCTAGAG 
TGGCAGCGTC AAACCCAGGA CTGGATAAAC TCTACAGAAC GCCAAGAAGA AACTCGGAAA 
GCCCCACACA AACAAGGGGT TGGATCAGAG GAGTCCCTGG TGCCCTACAG CAAGCCCAGC 
TTCCCGTCTC CTGGCGGGCA CAGCTCATCA GGAACATCTT CCTCTAAAGG GTCCACTGGC 
CCTCGGAAGG CTGACGTGCT GAGAGGCAGC CACCAGAGGA ATGCCAATGA CCTTCTCGAC 
ATTGGATATG TGGGCTCAAA TAGTCAAGGA CAGTTTACAG GTGAATTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523103.1
CDS	337..5007
Translation	MLSSEKVDKITRLELLFVLMLFFSGPTLGKLTRTPWKGKPWKGGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKAMSTDEGTYVCIAENRVGKVEASATLTVRVRPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPINQTLAVDGTALLKCKATGEPLPVISWLKEGFTFLGRDPRATIQDQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDMNDLPGPPSKPQVTDVSKNSVTLSWQPGTPGVLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVVVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQASTVWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSHNSTSISVSWDPPPADHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVVIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGGRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPNYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTTYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSVQQKTDLMGFGYSLPDQNKGNNGGKGGKKKKTKNSSKAQKNNGSTWANVPLPPPPVQPLPGTELGHYAAEQENGYDSDSWCPPLPVQTYLHQGMEDELEEDEDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNTPPPQPPAPPLGYVSGALISDLETDVPDEDADDEEEPLEIPRPLRALDQTPGSSMDNLDSSVTGSMVNGWGSASDEDRNFSSHRSSVGSSSDGSIFASGSFAKALVAAADKAGFRLDGTSLTRTGKAFSSSQRQRPTSPFSTDSNTSAAQNQSQRPRPTKKHKGGRMDPQPVLPHRREGMPDDLPPPPDPPPGQGLRQQIGLSQHSGNVENSTERKGSSLERQQAANLEDTKSSLDCPAKTVLEWQRQTQDWINSTERQEETRKAPHKQGVGSEESLVPYSKPSFPSPGGHSSSGTSSSKGSTGPRKADVLRGSHQRNANDLLDIGYVGSNSQGQFTGEL

Target ORF information:

RefSeq Version	XM_006523040.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523040.3

ORF Insert Sequence:

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT 
CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG 
GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC 
CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA 
GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT 
ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG 
GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG 
CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT 
AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT 
TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC 
GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC 
CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT 
ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA 
CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA 
TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA 
GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT 
CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT 
GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG 
CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG 
GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC 
ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG 
GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT 
GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA 
GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT 
GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG 
GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT 
GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT 
CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG 
CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG 
CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT 
TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG 
GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT 
GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT 
GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGGTG GGAAAGGTGG AAAAAAGAAG 
AAAACTAAAA ATTCTTCGAA AGCGCAGAAA AACAACGGAT CCACTTGGGC TAATGTCCCT 
CTACCTCCTC CTCCCGTCCA GCCCCTTCCT GGTACAGAGC TGGGCCACTA TGCTGCGGAA 
CAAGAAAATG GCTATGACAG TGACAGCTGG TGCCCACCAT TACCGGTGCA AACATACTTG 
CATCAGGGTA TGGAAGATGA GCTGGAAGAA GACGAAGATC GGGTCCCAAC ACCTCCTGTT 
CGCGGCGTGG CCTCTTCACC AGCTATCTCT TTTGGACAGC AGTCCACTGC CACTCTTACT 
CCATCCCCAC GGGAAGAGAT GCAACCCATG CTGCAAGCTC ACTTGGATGA GTTGACAAGG 
GCCTATCAGT TTGATATAGC AAAACAAACA TGGCACATTC AAAGCAATAC CCCACCTCCA 
CAACCCCCAG CTCCGCCATT AGGTTACGTG TCCGGAGCCT TGATTTCTGA TTTGGAGACA 
GATGTTCCAG ATGAGGATGC TGATGATGAA GAGGAACCAT TAGAAATTCC CAGGCCCCTC 
AGAGCACTAG ACCAGACACC TGGATCCAGT ATGGACAATC TAGACAGCTC TGTCACAGGT 
TCAATGGTAA ATGGATGGGG CTCTGCATCT GATGAGGATC GTAACTTTTC TAGTCATAGA 
TCTAGTGTAG GTAGCTCCTC AGATGGCTCC ATCTTTGCCA GCGGCAGCTT TGCAAAAGCA 
TTGGTGGCAG CAGCAGACAA AGCTGGCTTT AGGCTGGATG GAACCAGTCT TACGAGAACA 
GGAAAAGCCT TTAGCTCCTC TCAAAGGCAG CGGCCCACCA GCCCATTTTC TACGGACAGT 
AACACCAGTG CTGCCCAGAA TCAAAGCCAG AGGCCTCGGC CCACGAAAAA ACATAAGGGA 
GGACGGATGG ACCCACAGCC AGTGTTACCT CATCGGAGGG AAGGGATGCC AGATGATCTT 
CCCCCACCAC CAGATCCCCC ACCAGGTCAG GGTTTAAGGC AGCAGATAGG CCTGAGCCAA 
CACTCTGGTA ACGTGGAAAA CTCAACAGAG AGAAAAGGAA GCTCTCTAGA GAGACAACAA 
GCAGCCAACT TAGAAGACAC AAAGAGCTCA TTGGATTGTC CAGCTAAAAC TGTTCTAGAG 
TGGCAGCGTC AAACCCAGGA CTGGATAAAC TCTACAGAAC GCCAAGAAGA AACTCGGAAA 
GCCCCACACA AACAAGGGGT TGGATCAGAG GAGTCCCTGG TGCCCTACAG CAAGCCCAGC 
TTCCCGTCTC CTGGCGGGCA CAGCTCATCA GGAACATCTT CCTCTAAAGG GTCCACTGGC 
CCTCGGAAGG CTGACGTGCT GAGAGGCAGC CACCAGAGGA ATGCCAATGA CCTTCTCGAC 
ATTGGATATG TGGGCTCAAA TAGTCAAGGA CAGTTTACAG GTGAATTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo2 ( XM_006523041.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523104.1 Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X7, mRNA.

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CloneID	OMu52761
Clone ID Related Accession (Same CDS sequence)	XM_006523041.3 , XM_006523041.4
Accession Version	XM_006523041.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4614bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	roundabout homolog 2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006523041.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT 
CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG 
GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC 
CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA 
GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT 
ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG 
GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG 
CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT 
AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT 
TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC 
GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC 
CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT 
ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA 
CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA 
TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA 
GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT 
CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT 
GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG 
CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG 
GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC 
ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG 
GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT 
GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA 
GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT 
GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG 
GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT 
GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT 
CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG 
CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG 
CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT 
TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG 
GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT 
GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT 
GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGCCT TACTTTACAT CCCTGACTAC 
CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC 
ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA 
AAGAAGAAAA CTAAAAATTC TTCGAAAGCG CAGAAAAACA ACGGATCCAC TTGGGCTAAT 
GTCCCTCTAC CTCCTCCTCC CGTCCAGCCC CTTCCTGGTA CAGAGCTGGG CCACTATGCT 
GCGGAACAAG AAAATGGCTA TGACAGTGAC AGCTGGTGCC CACCATTACC GGTGCAAACA 
TACTTGCATC AGGGTATGGA AGATGAGCTG GAAGAAGACG AAGATCGGGT CCCAACACCT 
CCTGTTCGCG GCGTGGCCTC TTCACCAGCT ATCTCTTTTG GACAGCAGTC CACTGCCACT 
CTTACTCCAT CCCCACGGGA AGAGATGCAA CCCATGCTGC AAGCTCACTT GGATGAGTTG 
ACAAGGGCCT ATCAGTTTGA TATAGCAAAA CAAACATGGC ACATTCAAAG CAATACCCCA 
CCTCCACAAC CCCCAGCTCC GCCATTAGGT TACGTGTCCG GAGCCTTGAT TTCTGATTTG 
GAGACAGATG TTCCAGATGA GGATGCTGAT GATGAAGAGG AACCATTAGA AATTCCCAGG 
CCCCTCAGAG CACTAGACCA GACACCTGGA TCCAGTATGG ACAATCTAGA CAGCTCTGTC 
ACAGGAAAAG CCTTTAGCTC CTCTCAAAGG CAGCGGCCCA CCAGCCCATT TTCTACGGAC 
AGTAACACCA GTGCTGCCCA GAATCAAAGC CAGAGGCCTC GGCCCACGAA AAAACATAAG 
GGAGGACGGA TGGACCCACA GCCAGTGTTA CCTCATCGGA GGGAAGGGAT GCCAGATGAT 
CTTCCCCCAC CACCAGATCC CCCACCAGGT CAGGGTTTAA GGCAGCAGAT AGGCCTGAGC 
CAACACTCTG GTAACGTGGA AAACTCAACA GAGAGAAAAG GAAGCTCTCT AGAGAGACAA 
CAAGCAGCCA ACTTAGAAGA CACAAAGAGC TCATTGGATT GTCCAGCTAA AACTGTTCTA 
GAGTGGCAGC GTCAAACCCA GGACTGGATA AACTCTACAG AACGCCAAGA AGAAACTCGG 
AAAGCCCCAC ACAAACAAGG GGTTGGATCA GAGGAGTCCC TGGTGCCCTA CAGCAAGCCC 
AGCTTCCCGT CTCCTGGCGG GCACAGCTCA TCAGGAACAT CTTCCTCTAA AGGGTCCACT 
GGCCCTCGGA AGGCTGACGT GCTGAGAGGC AGCCACCAGA GGAATGCCAA TGACCTTCTC 
GACATTGGAT ATGTGGGCTC AAATAGTCAA GGACAGTTTA CAGGTGAATT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523104.1
CDS	337..4950
Translation	MLSSEKVDKITRLELLFVLMLFFSGPTLGKLTRTPWKGKPWKGGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKAMSTDEGTYVCIAENRVGKVEASATLTVRVRPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPINQTLAVDGTALLKCKATGEPLPVISWLKEGFTFLGRDPRATIQDQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDMNDLPGPPSKPQVTDVSKNSVTLSWQPGTPGVLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVVVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQASTVWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSHNSTSISVSWDPPPADHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVVIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGGRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPNYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTTYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSVQQKTDLMGFGYSLPDQNKGNNALLYIPDYRLAEGLSNRMPHNQSQDFSTTSSHNSSERSGSLSGGKGGKKKKTKNSSKAQKNNGSTWANVPLPPPPVQPLPGTELGHYAAEQENGYDSDSWCPPLPVQTYLHQGMEDELEEDEDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNTPPPQPPAPPLGYVSGALISDLETDVPDEDADDEEEPLEIPRPLRALDQTPGSSMDNLDSSVTGKAFSSSQRQRPTSPFSTDSNTSAAQNQSQRPRPTKKHKGGRMDPQPVLPHRREGMPDDLPPPPDPPPGQGLRQQIGLSQHSGNVENSTERKGSSLERQQAANLEDTKSSLDCPAKTVLEWQRQTQDWINSTERQEETRKAPHKQGVGSEESLVPYSKPSFPSPGGHSSSGTSSSKGSTGPRKADVLRGSHQRNANDLLDIGYVGSNSQGQFTGEL

Target ORF information:

RefSeq Version	XM_006523041.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523041.3

ORF Insert Sequence:

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT 
CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG 
GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC 
CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA 
GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT 
ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG 
GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG 
CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT 
AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT 
TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC 
GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC 
CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT 
ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA 
CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA 
TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA 
GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT 
CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT 
GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG 
CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG 
GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC 
ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG 
GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT 
GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA 
GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT 
GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG 
GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT 
GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT 
CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG 
CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG 
CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT 
TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG 
GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT 
GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT 
GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGCCT TACTTTACAT CCCTGACTAC 
CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC 
ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA 
AAGAAGAAAA CTAAAAATTC TTCGAAAGCG CAGAAAAACA ACGGATCCAC TTGGGCTAAT 
GTCCCTCTAC CTCCTCCTCC CGTCCAGCCC CTTCCTGGTA CAGAGCTGGG CCACTATGCT 
GCGGAACAAG AAAATGGCTA TGACAGTGAC AGCTGGTGCC CACCATTACC GGTGCAAACA 
TACTTGCATC AGGGTATGGA AGATGAGCTG GAAGAAGACG AAGATCGGGT CCCAACACCT 
CCTGTTCGCG GCGTGGCCTC TTCACCAGCT ATCTCTTTTG GACAGCAGTC CACTGCCACT 
CTTACTCCAT CCCCACGGGA AGAGATGCAA CCCATGCTGC AAGCTCACTT GGATGAGTTG 
ACAAGGGCCT ATCAGTTTGA TATAGCAAAA CAAACATGGC ACATTCAAAG CAATACCCCA 
CCTCCACAAC CCCCAGCTCC GCCATTAGGT TACGTGTCCG GAGCCTTGAT TTCTGATTTG 
GAGACAGATG TTCCAGATGA GGATGCTGAT GATGAAGAGG AACCATTAGA AATTCCCAGG 
CCCCTCAGAG CACTAGACCA GACACCTGGA TCCAGTATGG ACAATCTAGA CAGCTCTGTC 
ACAGGAAAAG CCTTTAGCTC CTCTCAAAGG CAGCGGCCCA CCAGCCCATT TTCTACGGAC 
AGTAACACCA GTGCTGCCCA GAATCAAAGC CAGAGGCCTC GGCCCACGAA AAAACATAAG 
GGAGGACGGA TGGACCCACA GCCAGTGTTA CCTCATCGGA GGGAAGGGAT GCCAGATGAT 
CTTCCCCCAC CACCAGATCC CCCACCAGGT CAGGGTTTAA GGCAGCAGAT AGGCCTGAGC 
CAACACTCTG GTAACGTGGA AAACTCAACA GAGAGAAAAG GAAGCTCTCT AGAGAGACAA 
CAAGCAGCCA ACTTAGAAGA CACAAAGAGC TCATTGGATT GTCCAGCTAA AACTGTTCTA 
GAGTGGCAGC GTCAAACCCA GGACTGGATA AACTCTACAG AACGCCAAGA AGAAACTCGG 
AAAGCCCCAC ACAAACAAGG GGTTGGATCA GAGGAGTCCC TGGTGCCCTA CAGCAAGCCC 
AGCTTCCCGT CTCCTGGCGG GCACAGCTCA TCAGGAACAT CTTCCTCTAA AGGGTCCACT 
GGCCCTCGGA AGGCTGACGT GCTGAGAGGC AGCCACCAGA GGAATGCCAA TGACCTTCTC 
GACATTGGAT ATGTGGGCTC AAATAGTCAA GGACAGTTTA CAGGTGAATT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo2 ( XM_006523042.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523105.1 Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OMu52762
Clone ID Related Accession (Same CDS sequence)	XM_006523042.3 , XM_006523042.4
Accession Version	XM_006523042.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	roundabout homolog 2 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006523042.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGCTCCTCC ACAGTTTGTG 
GTTAGGCCAA GAGATCAGAT CGTTGCTCAA GGCCGAACAG TGACATTCCC CTGTGAAACT 
AAAGGAAACC CACAGCCAGC TGTTTTTTGG CAGAAAGAAG GCAGCCAGAA CCTACTTTTC 
CCGAATCAAC CTCAGCAGCC CAACAGCCGA TGTTCAGTGT CGCCCACGGG GGACCTCACC 
ATCACCAACA TCCAGCGTTC AGATGCGGGT TACTACATCT GCCAGGCCCT AACCGTGGCA 
GGAAGCATTT TAGCTAAAGC ACAGTTGGAA GTTACTGACG TTTTGACAGA TAGACCTCCA 
CCCATAATCT TGCAAGGACC AATAAACCAA ACACTTGCAG TAGACGGTAC AGCATTGTTG 
AAGTGTAAAG CCACTGGTGA GCCTCTGCCT GTAATTAGCT GGCTAAAGGA GGGCTTTACT 
TTTCTGGGGA GAGATCCAAG AGCCACGATC CAAGACCAAG GAACACTGCA GATTAAGAAT 
TTACGGATAT CTGATACTGG CACTTATACT TGTGTGGCTA CAAGTTCCAG TGGAGAGACT 
TCCTGGAGTG CAGTGCTGGA TGTAACAGAA TCTGGAGCAA CAATCAGTAA AAATTATGAT 
ATGAATGACC TCCCGGGACC ACCATCCAAA CCTCAGGTCA CTGATGTTTC TAAGAACAGT 
GTCACCTTAT CCTGGCAGCC AGGTACACCT GGCGTTCTTC CTGCAAGCGC GTATATCATT 
GAGGCTTTCA GCCAATCGGT GAGCAATAGC TGGCAGACAG TGGCAAACCA TGTTAAGACA 
ACTCTGTATA CAGTAAGGGG GCTGAGGCCC AACACAATCT ACTTGTTTAT GGTCAGAGCG 
ATCAACCCAC AAGGTCTCAG TGATCCAAGT CCTATGTCGG ATCCTGTACG CACACAAGAT 
ATCAGCCCCC CAGCACAAGG AGTGGACCAC AGACAGGTGC AGAAGGAATT AGGTGATGTC 
GTTGTTCGTC TCCATAATCC AGTTGTCCTG ACACCTACAA CTGTTCAAGT CACATGGACG 
GTGGACCGAC AACCCCAGTT TATTCAGGGC TACAGAGTGA TGTACCGTCA GACTTCGGGA 
CTACAAGCCT CAACTGTGTG GCAGAATCTA GACGCCAAAG TCCCGACTGA GAGGAGTGCT 
GTCCTTGTGA ATTTGAAAAA GGGGGTGACT TATGAAATTA AAGTCCGGCC GTATTTTAAC 
GAGTTCCAAG GAATGGACAG TGAATCGAAA ACAGTCCGAA CCACTGAGGA AGCCCCAAGT 
GCCCCTCCCC AGTCTGTCAC TGTGCTGACA GTTGGAAGTC ACAACAGCAC AAGCATCAGT 
GTTTCCTGGG ATCCTCCACC AGCCGACCAC CAGAATGGAA TTATTCAGGA ATATAAGATC 
TGGTGTCTGG GAAACGAAAC GCGATTCCAT ATCAATAAAA CGGTGGATGC AGCCATTCGC 
TCTGTAGTAA TAGGTGGCTT GTTCCCTGGA ATTCAGTACC GGGTAGAAGT GGCAGCTAGC 
ACAAGTGCAG GGGTTGGAGT AAAAAGTGAA CCACAGCCGA TAATAATTGG GGGACGTAAC 
GAAGTTGTCA TTACTGAAAA CAATAACAGC ATCACTGAGC AAATCACGGA TGTCGTGAAG 
CAACCGGCAT TTATAGCTGG CATTGGTGGT GCCTGCTGGG TAATTCTGAT GGGTTTTAGC 
ATCTGGTTGT ACTGGAGAAG AAAGAAGAGA AAGGGACTCA GCAATTATGC TGTAACATTT 
CAAAGAGGAG ATGGAGGACT AATGAGCAAT GGGAGCCGTC CAGGTCTTCT AAATGCTGGC 
GATCCCAATT ACCCATGGCT TGCTGATTCT TGGCCAGCCA CGAGTTTGCC AGTGAACAAT 
AGCAATAGTG GCCCAAATGA AATTGGAAAT TTTGGGCGTG GAGATGTGCT GCCTCCGGTG 
CCAGGCCAAG GGGATAAAAC AGCGACCATG CTCTCGGATG GAGCCATTTA TAGCAGCATT 
GACTTCACTA CCAAAACCAC TTACAACAGT TCCAGCCAAA TAACACAGGC CACCCCATAT 
GCCACTACAC AAATCCTGCA TTCAAACAGC ATCCACGAAC TGGCAGTTGA TCTTCCTGAT 
CCACAGTGGA AAAGCTCAGT TCAACAGAAG ACAGACCTCA TGGGATTTGG TTATTCGCTA 
CCTGATCAGA ACAAGGGGAA CAACGCCTTA CTTTACATCC CTGACTACCG ATTGGCTGAG 
GGATTGTCTA ATAGAATGCC ACACAACCAG TCACAGGATT TCAGCACCAC CAGCTCTCAC 
AACAGCTCAG AAAGGAGTGG CAGTCTCTCA GGTGGGAAAG GTGGAAAAAA GAAGAAAACT 
AAAAATTCTT CGAAAGCGCA GAAAAACAAC GGATCCACTT GGGCTAATGT CCCTCTACCT 
CCTCCTCCCG TCCAGCCCCT TCCTGGTACA GAGCTGGGCC ACTATGCTGC GGAACAAGAA 
AATGGCTATG ACAGTGACAG CTGGTGCCCA CCATTACCGG TGCAAACATA CTTGCATCAG 
GGTATGGAAG ATGAGCTGGA AGAAGACGAA GATCGGGTCC CAACACCTCC TGTTCGCGGC 
GTGGCCTCTT CACCAGCTAT CTCTTTTGGA CAGCAGTCCA CTGCCACTCT TACTCCATCC 
CCACGGGAAG AGATGCAACC CATGCTGCAA GCTCACTTGG ATGAGTTGAC AAGGGCCTAT 
CAGTTTGATA TAGCAAAACA AACATGGCAC ATTCAAAGCA ATACCCCACC TCCACAACCC 
CCAGCTCCGC CATTAGGTTA CGTGTCCGGA GCCTTGATTT CTGATTTGGA GACAGATGTT 
CCAGATGAGG ATGCTGATGA TGAAGAGGAA CCATTAGAAA TTCCCAGGCC CCTCAGAGCA 
CTAGACCAGA CACCTGGATC CAGTATGGAC AATCTAGACA GCTCTGTCAC AGGAAAAGCC 
TTTAGCTCCT CTCAAAGGCA GCGGCCCACC AGCCCATTTT CTACGGACAG TAACACCAGT 
GCTGCCCAGA ATCAAAGCCA GAGGCCTCGG CCCACGAAAA AACATAAGGG AGGACGGATG 
GACCCACAGC CAGTGTTACC TCATCGGAGG GAAGGGATGC CAGATGATCT TCCCCCACCA 
CCAGATCCCC CACCAGGTCA GGGTTTAAGG CAGCAGATAG GCCTGAGCCA ACACTCTGGT 
AACGTGGAAA ACTCAACAGA GAGAAAAGGA AGCTCTCTAG AGAGACAACA AGCAGCCAAC 
TTAGAAGACA CAAAGAGCTC ATTGGATTGT CCAGCTAAAA CTGTTCTAGA GTGGCAGCGT 
CAAACCCAGG ACTGGATAAA CTCTACAGAA CGCCAAGAAG AAACTCGGAA AGCCCCACAC 
AAACAAGGGG TTGGATCAGA GGAGTCCCTG GTGCCCTACA GCAAGCCCAG CTTCCCGTCT 
CCTGGCGGGC ACAGCTCATC AGGAACATCT TCCTCTAAAG GGTCCACTGG CCCTCGGAAG 
GCTGACGTGC TGAGAGGCAG CCACCAGAGG AATGCCAATG ACCTTCTCGA CATTGGATAT 
GTGGGCTCAA ATAGTCAAGG ACAGTTTACA GGTGAATTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523105.1
CDS	337..4938
Translation	MLSSEKVDKITRLELLFVLMLFFSGPTLGKLTRTPWKGKPWKGGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKAMSTDEGTYVCIAENRVGKVEASATLTVRAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPINQTLAVDGTALLKCKATGEPLPVISWLKEGFTFLGRDPRATIQDQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDMNDLPGPPSKPQVTDVSKNSVTLSWQPGTPGVLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVVVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQASTVWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSHNSTSISVSWDPPPADHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVVIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGGRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPNYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTTYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSVQQKTDLMGFGYSLPDQNKGNNALLYIPDYRLAEGLSNRMPHNQSQDFSTTSSHNSSERSGSLSGGKGGKKKKTKNSSKAQKNNGSTWANVPLPPPPVQPLPGTELGHYAAEQENGYDSDSWCPPLPVQTYLHQGMEDELEEDEDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNTPPPQPPAPPLGYVSGALISDLETDVPDEDADDEEEPLEIPRPLRALDQTPGSSMDNLDSSVTGKAFSSSQRQRPTSPFSTDSNTSAAQNQSQRPRPTKKHKGGRMDPQPVLPHRREGMPDDLPPPPDPPPGQGLRQQIGLSQHSGNVENSTERKGSSLERQQAANLEDTKSSLDCPAKTVLEWQRQTQDWINSTERQEETRKAPHKQGVGSEESLVPYSKPSFPSPGGHSSSGTSSSKGSTGPRKADVLRGSHQRNANDLLDIGYVGSNSQGQFTGEL

Target ORF information:

RefSeq Version	XM_006523042.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523042.3

ORF Insert Sequence:

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGCTCCTCC ACAGTTTGTG 
GTTAGGCCAA GAGATCAGAT CGTTGCTCAA GGCCGAACAG TGACATTCCC CTGTGAAACT 
AAAGGAAACC CACAGCCAGC TGTTTTTTGG CAGAAAGAAG GCAGCCAGAA CCTACTTTTC 
CCGAATCAAC CTCAGCAGCC CAACAGCCGA TGTTCAGTGT CGCCCACGGG GGACCTCACC 
ATCACCAACA TCCAGCGTTC AGATGCGGGT TACTACATCT GCCAGGCCCT AACCGTGGCA 
GGAAGCATTT TAGCTAAAGC ACAGTTGGAA GTTACTGACG TTTTGACAGA TAGACCTCCA 
CCCATAATCT TGCAAGGACC AATAAACCAA ACACTTGCAG TAGACGGTAC AGCATTGTTG 
AAGTGTAAAG CCACTGGTGA GCCTCTGCCT GTAATTAGCT GGCTAAAGGA GGGCTTTACT 
TTTCTGGGGA GAGATCCAAG AGCCACGATC CAAGACCAAG GAACACTGCA GATTAAGAAT 
TTACGGATAT CTGATACTGG CACTTATACT TGTGTGGCTA CAAGTTCCAG TGGAGAGACT 
TCCTGGAGTG CAGTGCTGGA TGTAACAGAA TCTGGAGCAA CAATCAGTAA AAATTATGAT 
ATGAATGACC TCCCGGGACC ACCATCCAAA CCTCAGGTCA CTGATGTTTC TAAGAACAGT 
GTCACCTTAT CCTGGCAGCC AGGTACACCT GGCGTTCTTC CTGCAAGCGC GTATATCATT 
GAGGCTTTCA GCCAATCGGT GAGCAATAGC TGGCAGACAG TGGCAAACCA TGTTAAGACA 
ACTCTGTATA CAGTAAGGGG GCTGAGGCCC AACACAATCT ACTTGTTTAT GGTCAGAGCG 
ATCAACCCAC AAGGTCTCAG TGATCCAAGT CCTATGTCGG ATCCTGTACG CACACAAGAT 
ATCAGCCCCC CAGCACAAGG AGTGGACCAC AGACAGGTGC AGAAGGAATT AGGTGATGTC 
GTTGTTCGTC TCCATAATCC AGTTGTCCTG ACACCTACAA CTGTTCAAGT CACATGGACG 
GTGGACCGAC AACCCCAGTT TATTCAGGGC TACAGAGTGA TGTACCGTCA GACTTCGGGA 
CTACAAGCCT CAACTGTGTG GCAGAATCTA GACGCCAAAG TCCCGACTGA GAGGAGTGCT 
GTCCTTGTGA ATTTGAAAAA GGGGGTGACT TATGAAATTA AAGTCCGGCC GTATTTTAAC 
GAGTTCCAAG GAATGGACAG TGAATCGAAA ACAGTCCGAA CCACTGAGGA AGCCCCAAGT 
GCCCCTCCCC AGTCTGTCAC TGTGCTGACA GTTGGAAGTC ACAACAGCAC AAGCATCAGT 
GTTTCCTGGG ATCCTCCACC AGCCGACCAC CAGAATGGAA TTATTCAGGA ATATAAGATC 
TGGTGTCTGG GAAACGAAAC GCGATTCCAT ATCAATAAAA CGGTGGATGC AGCCATTCGC 
TCTGTAGTAA TAGGTGGCTT GTTCCCTGGA ATTCAGTACC GGGTAGAAGT GGCAGCTAGC 
ACAAGTGCAG GGGTTGGAGT AAAAAGTGAA CCACAGCCGA TAATAATTGG GGGACGTAAC 
GAAGTTGTCA TTACTGAAAA CAATAACAGC ATCACTGAGC AAATCACGGA TGTCGTGAAG 
CAACCGGCAT TTATAGCTGG CATTGGTGGT GCCTGCTGGG TAATTCTGAT GGGTTTTAGC 
ATCTGGTTGT ACTGGAGAAG AAAGAAGAGA AAGGGACTCA GCAATTATGC TGTAACATTT 
CAAAGAGGAG ATGGAGGACT AATGAGCAAT GGGAGCCGTC CAGGTCTTCT AAATGCTGGC 
GATCCCAATT ACCCATGGCT TGCTGATTCT TGGCCAGCCA CGAGTTTGCC AGTGAACAAT 
AGCAATAGTG GCCCAAATGA AATTGGAAAT TTTGGGCGTG GAGATGTGCT GCCTCCGGTG 
CCAGGCCAAG GGGATAAAAC AGCGACCATG CTCTCGGATG GAGCCATTTA TAGCAGCATT 
GACTTCACTA CCAAAACCAC TTACAACAGT TCCAGCCAAA TAACACAGGC CACCCCATAT 
GCCACTACAC AAATCCTGCA TTCAAACAGC ATCCACGAAC TGGCAGTTGA TCTTCCTGAT 
CCACAGTGGA AAAGCTCAGT TCAACAGAAG ACAGACCTCA TGGGATTTGG TTATTCGCTA 
CCTGATCAGA ACAAGGGGAA CAACGCCTTA CTTTACATCC CTGACTACCG ATTGGCTGAG 
GGATTGTCTA ATAGAATGCC ACACAACCAG TCACAGGATT TCAGCACCAC CAGCTCTCAC 
AACAGCTCAG AAAGGAGTGG CAGTCTCTCA GGTGGGAAAG GTGGAAAAAA GAAGAAAACT 
AAAAATTCTT CGAAAGCGCA GAAAAACAAC GGATCCACTT GGGCTAATGT CCCTCTACCT 
CCTCCTCCCG TCCAGCCCCT TCCTGGTACA GAGCTGGGCC ACTATGCTGC GGAACAAGAA 
AATGGCTATG ACAGTGACAG CTGGTGCCCA CCATTACCGG TGCAAACATA CTTGCATCAG 
GGTATGGAAG ATGAGCTGGA AGAAGACGAA GATCGGGTCC CAACACCTCC TGTTCGCGGC 
GTGGCCTCTT CACCAGCTAT CTCTTTTGGA CAGCAGTCCA CTGCCACTCT TACTCCATCC 
CCACGGGAAG AGATGCAACC CATGCTGCAA GCTCACTTGG ATGAGTTGAC AAGGGCCTAT 
CAGTTTGATA TAGCAAAACA AACATGGCAC ATTCAAAGCA ATACCCCACC TCCACAACCC 
CCAGCTCCGC CATTAGGTTA CGTGTCCGGA GCCTTGATTT CTGATTTGGA GACAGATGTT 
CCAGATGAGG ATGCTGATGA TGAAGAGGAA CCATTAGAAA TTCCCAGGCC CCTCAGAGCA 
CTAGACCAGA CACCTGGATC CAGTATGGAC AATCTAGACA GCTCTGTCAC AGGAAAAGCC 
TTTAGCTCCT CTCAAAGGCA GCGGCCCACC AGCCCATTTT CTACGGACAG TAACACCAGT 
GCTGCCCAGA ATCAAAGCCA GAGGCCTCGG CCCACGAAAA AACATAAGGG AGGACGGATG 
GACCCACAGC CAGTGTTACC TCATCGGAGG GAAGGGATGC CAGATGATCT TCCCCCACCA 
CCAGATCCCC CACCAGGTCA GGGTTTAAGG CAGCAGATAG GCCTGAGCCA ACACTCTGGT 
AACGTGGAAA ACTCAACAGA GAGAAAAGGA AGCTCTCTAG AGAGACAACA AGCAGCCAAC 
TTAGAAGACA CAAAGAGCTC ATTGGATTGT CCAGCTAAAA CTGTTCTAGA GTGGCAGCGT 
CAAACCCAGG ACTGGATAAA CTCTACAGAA CGCCAAGAAG AAACTCGGAA AGCCCCACAC 
AAACAAGGGG TTGGATCAGA GGAGTCCCTG GTGCCCTACA GCAAGCCCAG CTTCCCGTCT 
CCTGGCGGGC ACAGCTCATC AGGAACATCT TCCTCTAAAG GGTCCACTGG CCCTCGGAAG 
GCTGACGTGC TGAGAGGCAG CCACCAGAGG AATGCCAATG ACCTTCTCGA CATTGGATAT 
GTGGGCTCAA ATAGTCAAGG ACAGTTTACA GGTGAATTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo2 ( XM_006523043.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523106.1 Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OMu52763
Clone ID Related Accession (Same CDS sequence)	XM_006523043.3 , XM_006523043.4
Accession Version	XM_006523043.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	roundabout homolog 2 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006523043.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCCTC TGATGTTTAC ACTATTATTG CTCTTTGGAT TTCTCTGCAT TCAGATTGAT 
GGATCTCGTC TTCGTCAAGA AGACTTTCCC CCCAGGATCG TAGAACACCC TTCTGATGTC 
ATCGTCTCCA AGGGAGAGCC CACCACTCTG AACTGTAAAG CAGAGGGCCG ACCCACCCCC 
ACCATTGAAT GGTACAAGGA TGGTGAGAGG GTGGAGACAG ACAAGGATGA TCCCAGGTCC 
CACAGAATGC TTCTGCCCAG CGGATCTTTA TTCTTTTTGC GAATTGTTCA TGGGCGCAGA 
AGTAAACCGG ACGAAGGGAG TTACGTTTGT GTTGCAAGGA ACTATCTTGG TGAAGCAGTG 
AGTCGAAATG CATCTCTGGA AGTGGCATTA TTGCGAGATG ACTTCCGGCA AAACCCCACA 
GATGTGGTAG TCGCAGCTGG AGAGCCTGCA ATCTTGGAGT GCCAGCCACC ACGGGGACAC 
CCAGAACCAA CCATCTACTG GAAAAAGGAC AAAGTCCGAA TTGATGACAA GGAAGAGAGA 
ATAAGTATCC GTGGTGGGAA GCTGATGATC TCTAATACTA GGAAAAGCGA TGCTGGCATG 
TACACCTGTG TGGGAACCAA TATGGTGGGA GAAAGGGACA GCGACCCTGC AGAGCTCACT 
GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTGCT AGAGGAAGAA 
GCTGTAGAAT TCCGTTGTCA GGTCCAAGGA GATCCCCAGC CAACGGTGAG GTGGAAAAAA 
GATGATGCAG ACTTGCCGAG AGGAAGGTAT GATATCAAAG ATGACTACAC GCTGAGAATT 
AAAAAGGCCA TGAGTACTGA TGAAGGTACC TATGTGTGTA TTGCTGAGAA TCGGGTGGGA 
AAAGTGGAAG CCTCTGCTAC CCTCACTGTC CGAGTTCGCC CTGTTGCTCC TCCACAGTTT 
GTGGTTAGGC CAAGAGATCA GATCGTTGCT CAAGGCCGAA CAGTGACATT CCCCTGTGAA 
ACTAAAGGAA ACCCACAGCC AGCTGTTTTT TGGCAGAAAG AAGGCAGCCA GAACCTACTT 
TTCCCGAATC AACCTCAGCA GCCCAACAGC CGATGTTCAG TGTCGCCCAC GGGGGACCTC 
ACCATCACCA ACATCCAGCG TTCAGATGCG GGTTACTACA TCTGCCAGGC CCTAACCGTG 
GCAGGAAGCA TTTTAGCTAA AGCACAGTTG GAAGTTACTG ACGTTTTGAC AGATAGACCT 
CCACCCATAA TCTTGCAAGG ACCAATAAAC CAAACACTTG CAGTAGACGG TACAGCATTG 
TTGAAGTGTA AAGCCACTGG TGAGCCTCTG CCTGTAATTA GCTGGCTAAA GGAGGGCTTT 
ACTTTTCTGG GGAGAGATCC AAGAGCCACG ATCCAAGACC AAGGAACACT GCAGATTAAG 
AATTTACGGA TATCTGATAC TGGCACTTAT ACTTGTGTGG CTACAAGTTC CAGTGGAGAG 
ACTTCCTGGA GTGCAGTGCT GGATGTAACA GAATCTGGAG CAACAATCAG TAAAAATTAT 
GATATGAATG ACCTCCCGGG ACCACCATCC AAACCTCAGG TCACTGATGT TTCTAAGAAC 
AGTGTCACCT TATCCTGGCA GCCAGGTACA CCTGGCGTTC TTCCTGCAAG CGCGTATATC 
ATTGAGGCTT TCAGCCAATC GGTGAGCAAT AGCTGGCAGA CAGTGGCAAA CCATGTTAAG 
ACAACTCTGT ATACAGTAAG GGGGCTGAGG CCCAACACAA TCTACTTGTT TATGGTCAGA 
GCGATCAACC CACAAGGTCT CAGTGATCCA AGTCCTATGT CGGATCCTGT ACGCACACAA 
GATATCAGCC CCCCAGCACA AGGAGTGGAC CACAGACAGG TGCAGAAGGA ATTAGGTGAT 
GTCGTTGTTC GTCTCCATAA TCCAGTTGTC CTGACACCTA CAACTGTTCA AGTCACATGG 
ACGGTGGACC GACAACCCCA GTTTATTCAG GGCTACAGAG TGATGTACCG TCAGACTTCG 
GGACTACAAG CCTCAACTGT GTGGCAGAAT CTAGACGCCA AAGTCCCGAC TGAGAGGAGT 
GCTGTCCTTG TGAATTTGAA AAAGGGGGTG ACTTATGAAA TTAAAGTCCG GCCGTATTTT 
AACGAGTTCC AAGGAATGGA CAGTGAATCG AAAACAGTCC GAACCACTGA GGAAGCCCCA 
AGTGCCCCTC CCCAGTCTGT CACTGTGCTG ACAGTTGGAA GTCACAACAG CACAAGCATC 
AGTGTTTCCT GGGATCCTCC ACCAGCCGAC CACCAGAATG GAATTATTCA GGAATATAAG 
ATCTGGTGTC TGGGAAACGA AACGCGATTC CATATCAATA AAACGGTGGA TGCAGCCATT 
CGCTCTGTAG TAATAGGTGG CTTGTTCCCT GGAATTCAGT ACCGGGTAGA AGTGGCAGCT 
AGCACAAGTG CAGGGGTTGG AGTAAAAAGT GAACCACAGC CGATAATAAT TGGGGGACGT 
AACGAAGTTG TCATTACTGA AAACAATAAC AGCATCACTG AGCAAATCAC GGATGTCGTG 
AAGCAACCGG CATTTATAGC TGGCATTGGT GGTGCCTGCT GGGTAATTCT GATGGGTTTT 
AGCATCTGGT TGTACTGGAG AAGAAAGAAG AGAAAGGGAC TCAGCAATTA TGCTGTAACA 
TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGGAGCC GTCCAGGTCT TCTAAATGCT 
GGCGATCCCA ATTACCCATG GCTTGCTGAT TCTTGGCCAG CCACGAGTTT GCCAGTGAAC 
AATAGCAATA GTGGCCCAAA TGAAATTGGA AATTTTGGGC GTGGAGATGT GCTGCCTCCG 
GTGCCAGGCC AAGGGGATAA AACAGCGACC ATGCTCTCGG ATGGAGCCAT TTATAGCAGC 
ATTGACTTCA CTACCAAAAC CACTTACAAC AGTTCCAGCC AAATAACACA GGCCACCCCA 
TATGCCACTA CACAAATCCT GCATTCAAAC AGCATCCACG AACTGGCAGT TGATCTTCCT 
GATCCACAGT GGAAAAGCTC AGTTCAACAG AAGACAGACC TCATGGGATT TGGTTATTCG 
CTACCTGATC AGAACAAGGG GAACAACGGT GGGAAAGGTG GAAAAAAGAA GAAAACTAAA 
AATTCTTCGA AAGCGCAGAA AAACAACGGA TCCACTTGGG CTAATGTCCC TCTACCTCCT 
CCTCCCGTCC AGCCCCTTCC TGGTACAGAG CTGGGCCACT ATGCTGCGGA ACAAGAAAAT 
GGCTATGACA GTGACAGCTG GTGCCCACCA TTACCGGTGC AAACATACTT GCATCAGGGT 
ATGGAAGATG AGCTGGAAGA AGACGAAGAT CGGGTCCCAA CACCTCCTGT TCGCGGCGTG 
GCCTCTTCAC CAGCTATCTC TTTTGGACAG CAGTCCACTG CCACTCTTAC TCCATCCCCA 
CGGGAAGAGA TGCAACCCAT GCTGCAAGCT CACTTGGATG AGTTGACAAG GGCCTATCAG 
TTTGATATAG CAAAACAAAC ATGGCACATT CAAAGCAATA CCCCACCTCC ACAACCCCCA 
GCTCCGCCAT TAGGTTACGT GTCCGGAGCC TTGATTTCTG ATTTGGAGAC AGATGTTCCA 
GATGAGGATG CTGATGATGA AGAGGAACCA TTAGAAATTC CCAGGCCCCT CAGAGCACTA 
GACCAGACAC CTGGATCCAG TATGGACAAT CTAGACAGCT CTGTCACAGG TTCAATGGTA 
AATGGATGGG GCTCTGCATC TGATGAGGAT CGTAACTTTT CTAGTCATAG ATCTAGTGTA 
GGTAGCTCCT CAGATGGCTC CATCTTTGCC AGCGGCAGCT TTGCAAAAGC ATTGGTGGCA 
GCAGCAGACA AAGCTGGCTT TAGGCTGGAT GGAACCAGTC TTACGAGAAC AGGAAAAGCC 
TTTAGCTCCT CTCAAAGGCA GCGGCCCACC AGCCCATTTT CTACGGACAG TAACACCAGT 
GCTGCCCAGA ATCAAAGCCA GAGGCCTCGG CCCACGAAAA AACATAAGGG AGGACGGATG 
GACCCACAGC CAGTGTTACC TCATCGGAGG GAAGGGATGC CAGATGATCT TCCCCCACCA 
CCAGATCCCC CACCAGGTCA GGGTTTAAGG CAGCAGATAG GCCTGAGCCA ACACTCTGGT 
AACGTGGAAA ACTCAACAGA GAGAAAAGGA AGCTCTCTAG AGAGACAACA AGCAGCCAAC 
TTAGAAGACA CAAAGAGCTC ATTGGATTGT CCAGCTAAAA CTGTTCTAGA GTGGCAGCGT 
CAAACCCAGG ACTGGATAAA CTCTACAGAA CGCCAAGAAG AAACTCGGAA AGCCCCACAC 
AAACAAGGGG TTGGATCAGA GGAGTCCCTG GTGCCCTACA GCAAGCCCAG CTTCCCGTCT 
CCTGGCGGGC ACAGCTCATC AGGAACATCT TCCTCTAAAG GGTCCACTGG CCCTCGGAAG 
GCTGACGTGC TGAGAGGCAG CCACCAGAGG AATGCCAATG ACCTTCTCGA CATTGGATAT 
GTGGGCTCAA ATAGTCAAGG ACAGTTTACA GGTGAATTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523106.1
CDS	874..5475
Translation	MNPLMFTLLLLFGFLCIQIDGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKAMSTDEGTYVCIAENRVGKVEASATLTVRVRPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPINQTLAVDGTALLKCKATGEPLPVISWLKEGFTFLGRDPRATIQDQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDMNDLPGPPSKPQVTDVSKNSVTLSWQPGTPGVLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVVVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQASTVWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSHNSTSISVSWDPPPADHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVVIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGGRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPNYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTTYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSVQQKTDLMGFGYSLPDQNKGNNGGKGGKKKKTKNSSKAQKNNGSTWANVPLPPPPVQPLPGTELGHYAAEQENGYDSDSWCPPLPVQTYLHQGMEDELEEDEDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNTPPPQPPAPPLGYVSGALISDLETDVPDEDADDEEEPLEIPRPLRALDQTPGSSMDNLDSSVTGSMVNGWGSASDEDRNFSSHRSSVGSSSDGSIFASGSFAKALVAAADKAGFRLDGTSLTRTGKAFSSSQRQRPTSPFSTDSNTSAAQNQSQRPRPTKKHKGGRMDPQPVLPHRREGMPDDLPPPPDPPPGQGLRQQIGLSQHSGNVENSTERKGSSLERQQAANLEDTKSSLDCPAKTVLEWQRQTQDWINSTERQEETRKAPHKQGVGSEESLVPYSKPSFPSPGGHSSSGTSSSKGSTGPRKADVLRGSHQRNANDLLDIGYVGSNSQGQFTGEL

Target ORF information:

RefSeq Version	XM_006523043.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523043.3

ORF Insert Sequence:

ATGAATCCTC TGATGTTTAC ACTATTATTG CTCTTTGGAT TTCTCTGCAT TCAGATTGAT 
GGATCTCGTC TTCGTCAAGA AGACTTTCCC CCCAGGATCG TAGAACACCC TTCTGATGTC 
ATCGTCTCCA AGGGAGAGCC CACCACTCTG AACTGTAAAG CAGAGGGCCG ACCCACCCCC 
ACCATTGAAT GGTACAAGGA TGGTGAGAGG GTGGAGACAG ACAAGGATGA TCCCAGGTCC 
CACAGAATGC TTCTGCCCAG CGGATCTTTA TTCTTTTTGC GAATTGTTCA TGGGCGCAGA 
AGTAAACCGG ACGAAGGGAG TTACGTTTGT GTTGCAAGGA ACTATCTTGG TGAAGCAGTG 
AGTCGAAATG CATCTCTGGA AGTGGCATTA TTGCGAGATG ACTTCCGGCA AAACCCCACA 
GATGTGGTAG TCGCAGCTGG AGAGCCTGCA ATCTTGGAGT GCCAGCCACC ACGGGGACAC 
CCAGAACCAA CCATCTACTG GAAAAAGGAC AAAGTCCGAA TTGATGACAA GGAAGAGAGA 
ATAAGTATCC GTGGTGGGAA GCTGATGATC TCTAATACTA GGAAAAGCGA TGCTGGCATG 
TACACCTGTG TGGGAACCAA TATGGTGGGA GAAAGGGACA GCGACCCTGC AGAGCTCACT 
GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTGCT AGAGGAAGAA 
GCTGTAGAAT TCCGTTGTCA GGTCCAAGGA GATCCCCAGC CAACGGTGAG GTGGAAAAAA 
GATGATGCAG ACTTGCCGAG AGGAAGGTAT GATATCAAAG ATGACTACAC GCTGAGAATT 
AAAAAGGCCA TGAGTACTGA TGAAGGTACC TATGTGTGTA TTGCTGAGAA TCGGGTGGGA 
AAAGTGGAAG CCTCTGCTAC CCTCACTGTC CGAGTTCGCC CTGTTGCTCC TCCACAGTTT 
GTGGTTAGGC CAAGAGATCA GATCGTTGCT CAAGGCCGAA CAGTGACATT CCCCTGTGAA 
ACTAAAGGAA ACCCACAGCC AGCTGTTTTT TGGCAGAAAG AAGGCAGCCA GAACCTACTT 
TTCCCGAATC AACCTCAGCA GCCCAACAGC CGATGTTCAG TGTCGCCCAC GGGGGACCTC 
ACCATCACCA ACATCCAGCG TTCAGATGCG GGTTACTACA TCTGCCAGGC CCTAACCGTG 
GCAGGAAGCA TTTTAGCTAA AGCACAGTTG GAAGTTACTG ACGTTTTGAC AGATAGACCT 
CCACCCATAA TCTTGCAAGG ACCAATAAAC CAAACACTTG CAGTAGACGG TACAGCATTG 
TTGAAGTGTA AAGCCACTGG TGAGCCTCTG CCTGTAATTA GCTGGCTAAA GGAGGGCTTT 
ACTTTTCTGG GGAGAGATCC AAGAGCCACG ATCCAAGACC AAGGAACACT GCAGATTAAG 
AATTTACGGA TATCTGATAC TGGCACTTAT ACTTGTGTGG CTACAAGTTC CAGTGGAGAG 
ACTTCCTGGA GTGCAGTGCT GGATGTAACA GAATCTGGAG CAACAATCAG TAAAAATTAT 
GATATGAATG ACCTCCCGGG ACCACCATCC AAACCTCAGG TCACTGATGT TTCTAAGAAC 
AGTGTCACCT TATCCTGGCA GCCAGGTACA CCTGGCGTTC TTCCTGCAAG CGCGTATATC 
ATTGAGGCTT TCAGCCAATC GGTGAGCAAT AGCTGGCAGA CAGTGGCAAA CCATGTTAAG 
ACAACTCTGT ATACAGTAAG GGGGCTGAGG CCCAACACAA TCTACTTGTT TATGGTCAGA 
GCGATCAACC CACAAGGTCT CAGTGATCCA AGTCCTATGT CGGATCCTGT ACGCACACAA 
GATATCAGCC CCCCAGCACA AGGAGTGGAC CACAGACAGG TGCAGAAGGA ATTAGGTGAT 
GTCGTTGTTC GTCTCCATAA TCCAGTTGTC CTGACACCTA CAACTGTTCA AGTCACATGG 
ACGGTGGACC GACAACCCCA GTTTATTCAG GGCTACAGAG TGATGTACCG TCAGACTTCG 
GGACTACAAG CCTCAACTGT GTGGCAGAAT CTAGACGCCA AAGTCCCGAC TGAGAGGAGT 
GCTGTCCTTG TGAATTTGAA AAAGGGGGTG ACTTATGAAA TTAAAGTCCG GCCGTATTTT 
AACGAGTTCC AAGGAATGGA CAGTGAATCG AAAACAGTCC GAACCACTGA GGAAGCCCCA 
AGTGCCCCTC CCCAGTCTGT CACTGTGCTG ACAGTTGGAA GTCACAACAG CACAAGCATC 
AGTGTTTCCT GGGATCCTCC ACCAGCCGAC CACCAGAATG GAATTATTCA GGAATATAAG 
ATCTGGTGTC TGGGAAACGA AACGCGATTC CATATCAATA AAACGGTGGA TGCAGCCATT 
CGCTCTGTAG TAATAGGTGG CTTGTTCCCT GGAATTCAGT ACCGGGTAGA AGTGGCAGCT 
AGCACAAGTG CAGGGGTTGG AGTAAAAAGT GAACCACAGC CGATAATAAT TGGGGGACGT 
AACGAAGTTG TCATTACTGA AAACAATAAC AGCATCACTG AGCAAATCAC GGATGTCGTG 
AAGCAACCGG CATTTATAGC TGGCATTGGT GGTGCCTGCT GGGTAATTCT GATGGGTTTT 
AGCATCTGGT TGTACTGGAG AAGAAAGAAG AGAAAGGGAC TCAGCAATTA TGCTGTAACA 
TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGGAGCC GTCCAGGTCT TCTAAATGCT 
GGCGATCCCA ATTACCCATG GCTTGCTGAT TCTTGGCCAG CCACGAGTTT GCCAGTGAAC 
AATAGCAATA GTGGCCCAAA TGAAATTGGA AATTTTGGGC GTGGAGATGT GCTGCCTCCG 
GTGCCAGGCC AAGGGGATAA AACAGCGACC ATGCTCTCGG ATGGAGCCAT TTATAGCAGC 
ATTGACTTCA CTACCAAAAC CACTTACAAC AGTTCCAGCC AAATAACACA GGCCACCCCA 
TATGCCACTA CACAAATCCT GCATTCAAAC AGCATCCACG AACTGGCAGT TGATCTTCCT 
GATCCACAGT GGAAAAGCTC AGTTCAACAG AAGACAGACC TCATGGGATT TGGTTATTCG 
CTACCTGATC AGAACAAGGG GAACAACGGT GGGAAAGGTG GAAAAAAGAA GAAAACTAAA 
AATTCTTCGA AAGCGCAGAA AAACAACGGA TCCACTTGGG CTAATGTCCC TCTACCTCCT 
CCTCCCGTCC AGCCCCTTCC TGGTACAGAG CTGGGCCACT ATGCTGCGGA ACAAGAAAAT 
GGCTATGACA GTGACAGCTG GTGCCCACCA TTACCGGTGC AAACATACTT GCATCAGGGT 
ATGGAAGATG AGCTGGAAGA AGACGAAGAT CGGGTCCCAA CACCTCCTGT TCGCGGCGTG 
GCCTCTTCAC CAGCTATCTC TTTTGGACAG CAGTCCACTG CCACTCTTAC TCCATCCCCA 
CGGGAAGAGA TGCAACCCAT GCTGCAAGCT CACTTGGATG AGTTGACAAG GGCCTATCAG 
TTTGATATAG CAAAACAAAC ATGGCACATT CAAAGCAATA CCCCACCTCC ACAACCCCCA 
GCTCCGCCAT TAGGTTACGT GTCCGGAGCC TTGATTTCTG ATTTGGAGAC AGATGTTCCA 
GATGAGGATG CTGATGATGA AGAGGAACCA TTAGAAATTC CCAGGCCCCT CAGAGCACTA 
GACCAGACAC CTGGATCCAG TATGGACAAT CTAGACAGCT CTGTCACAGG TTCAATGGTA 
AATGGATGGG GCTCTGCATC TGATGAGGAT CGTAACTTTT CTAGTCATAG ATCTAGTGTA 
GGTAGCTCCT CAGATGGCTC CATCTTTGCC AGCGGCAGCT TTGCAAAAGC ATTGGTGGCA 
GCAGCAGACA AAGCTGGCTT TAGGCTGGAT GGAACCAGTC TTACGAGAAC AGGAAAAGCC 
TTTAGCTCCT CTCAAAGGCA GCGGCCCACC AGCCCATTTT CTACGGACAG TAACACCAGT 
GCTGCCCAGA ATCAAAGCCA GAGGCCTCGG CCCACGAAAA AACATAAGGG AGGACGGATG 
GACCCACAGC CAGTGTTACC TCATCGGAGG GAAGGGATGC CAGATGATCT TCCCCCACCA 
CCAGATCCCC CACCAGGTCA GGGTTTAAGG CAGCAGATAG GCCTGAGCCA ACACTCTGGT 
AACGTGGAAA ACTCAACAGA GAGAAAAGGA AGCTCTCTAG AGAGACAACA AGCAGCCAAC 
TTAGAAGACA CAAAGAGCTC ATTGGATTGT CCAGCTAAAA CTGTTCTAGA GTGGCAGCGT 
CAAACCCAGG ACTGGATAAA CTCTACAGAA CGCCAAGAAG AAACTCGGAA AGCCCCACAC 
AAACAAGGGG TTGGATCAGA GGAGTCCCTG GTGCCCTACA GCAAGCCCAG CTTCCCGTCT 
CCTGGCGGGC ACAGCTCATC AGGAACATCT TCCTCTAAAG GGTCCACTGG CCCTCGGAAG 
GCTGACGTGC TGAGAGGCAG CCACCAGAGG AATGCCAATG ACCTTCTCGA CATTGGATAT 
GTGGGCTCAA ATAGTCAAGG ACAGTTTACA GGTGAATTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo2 ( XM_006523044.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523107.1 Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X11, mRNA.

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CloneID	OMu52764
Clone ID Related Accession (Same CDS sequence)	XM_006523044.3 , NM_001358490.1
Accession Version	XM_006523044.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4584bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	roundabout homolog 2 isoform X11
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006523044.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGAA GACAAGAAAG TATCTGTGGA AGGCCGTGGA CGTGGACGCC AGGACTACTG 
ATGCTGATCA TCCTTGGCAT TCATCAGGGG AGTGGGCAAG GTCAAGGATC TCGTCTTCGT 
CAAGAAGACT TTCCCCCCAG GATCGTAGAA CACCCTTCTG ATGTCATCGT CTCCAAGGGA 
GAGCCCACCA CTCTGAACTG TAAAGCAGAG GGCCGACCCA CCCCCACCAT TGAATGGTAC 
AAGGATGGTG AGAGGGTGGA GACAGACAAG GATGATCCCA GGTCCCACAG AATGCTTCTG 
CCCAGCGGAT CTTTATTCTT TTTGCGAATT GTTCATGGGC GCAGAAGTAA ACCGGACGAA 
GGGAGTTACG TTTGTGTTGC AAGGAACTAT CTTGGTGAAG CAGTGAGTCG AAATGCATCT 
CTGGAAGTGG CATTATTGCG AGATGACTTC CGGCAAAACC CCACAGATGT GGTAGTCGCA 
GCTGGAGAGC CTGCAATCTT GGAGTGCCAG CCACCACGGG GACACCCAGA ACCAACCATC 
TACTGGAAAA AGGACAAAGT CCGAATTGAT GACAAGGAAG AGAGAATAAG TATCCGTGGT 
GGGAAGCTGA TGATCTCTAA TACTAGGAAA AGCGATGCTG GCATGTACAC CTGTGTGGGA 
ACCAATATGG TGGGAGAAAG GGACAGCGAC CCTGCAGAGC TCACTGTCTT TGAACGACCC 
ACATTTCTCA GGAGGCCAAT TAACCAGGTG GTGCTAGAGG AAGAAGCTGT AGAATTCCGT 
TGTCAGGTCC AAGGAGATCC CCAGCCAACG GTGAGGTGGA AAAAAGATGA TGCAGACTTG 
CCGAGAGGAA GGTATGATAT CAAAGATGAC TACACGCTGA GAATTAAAAA GGCCATGAGT 
ACTGATGAAG GTACCTATGT GTGTATTGCT GAGAATCGGG TGGGAAAAGT GGAAGCCTCT 
GCTACCCTCA CTGTCCGAGT TCGCCCTGTT GCTCCTCCAC AGTTTGTGGT TAGGCCAAGA 
GATCAGATCG TTGCTCAAGG CCGAACAGTG ACATTCCCCT GTGAAACTAA AGGAAACCCA 
CAGCCAGCTG TTTTTTGGCA GAAAGAAGGC AGCCAGAACC TACTTTTCCC GAATCAACCT 
CAGCAGCCCA ACAGCCGATG TTCAGTGTCG CCCACGGGGG ACCTCACCAT CACCAACATC 
CAGCGTTCAG ATGCGGGTTA CTACATCTGC CAGGCCCTAA CCGTGGCAGG AAGCATTTTA 
GCTAAAGCAC AGTTGGAAGT TACTGACGTT TTGACAGATA GACCTCCACC CATAATCTTG 
CAAGGACCAA TAAACCAAAC ACTTGCAGTA GACGGTACAG CATTGTTGAA GTGTAAAGCC 
ACTGGTGAGC CTCTGCCTGT AATTAGCTGG CTAAAGGAGG GCTTTACTTT TCTGGGGAGA 
GATCCAAGAG CCACGATCCA AGACCAAGGA ACACTGCAGA TTAAGAATTT ACGGATATCT 
GATACTGGCA CTTATACTTG TGTGGCTACA AGTTCCAGTG GAGAGACTTC CTGGAGTGCA 
GTGCTGGATG TAACAGAATC TGGAGCAACA ATCAGTAAAA ATTATGATAT GAATGACCTC 
CCGGGACCAC CATCCAAACC TCAGGTCACT GATGTTTCTA AGAACAGTGT CACCTTATCC 
TGGCAGCCAG GTACACCTGG CGTTCTTCCT GCAAGCGCGT ATATCATTGA GGCTTTCAGC 
CAATCGGTGA GCAATAGCTG GCAGACAGTG GCAAACCATG TTAAGACAAC TCTGTATACA 
GTAAGGGGGC TGAGGCCCAA CACAATCTAC TTGTTTATGG TCAGAGCGAT CAACCCACAA 
GGTCTCAGTG ATCCAAGTCC TATGTCGGAT CCTGTACGCA CACAAGATAT CAGCCCCCCA 
GCACAAGGAG TGGACCACAG ACAGGTGCAG AAGGAATTAG GTGATGTCGT TGTTCGTCTC 
CATAATCCAG TTGTCCTGAC ACCTACAACT GTTCAAGTCA CATGGACGGT GGACCGACAA 
CCCCAGTTTA TTCAGGGCTA CAGAGTGATG TACCGTCAGA CTTCGGGACT ACAAGCCTCA 
ACTGTGTGGC AGAATCTAGA CGCCAAAGTC CCGACTGAGA GGAGTGCTGT CCTTGTGAAT 
TTGAAAAAGG GGGTGACTTA TGAAATTAAA GTCCGGCCGT ATTTTAACGA GTTCCAAGGA 
ATGGACAGTG AATCGAAAAC AGTCCGAACC ACTGAGGAAG CCCCAAGTGC CCCTCCCCAG 
TCTGTCACTG TGCTGACAGT TGGAAGTCAC AACAGCACAA GCATCAGTGT TTCCTGGGAT 
CCTCCACCAG CCGACCACCA GAATGGAATT ATTCAGGAAT ATAAGATCTG GTGTCTGGGA 
AACGAAACGC GATTCCATAT CAATAAAACG GTGGATGCAG CCATTCGCTC TGTAGTAATA 
GGTGGCTTGT TCCCTGGAAT TCAGTACCGG GTAGAAGTGG CAGCTAGCAC AAGTGCAGGG 
GTTGGAGTAA AAAGTGAACC ACAGCCGATA ATAATTGGGG GACGTAACGA AGTTGTCATT 
ACTGAAAACA ATAACAGCAT CACTGAGCAA ATCACGGATG TCGTGAAGCA ACCGGCATTT 
ATAGCTGGCA TTGGTGGTGC CTGCTGGGTA ATTCTGATGG GTTTTAGCAT CTGGTTGTAC 
TGGAGAAGAA AGAAGAGAAA GGGACTCAGC AATTATGCTG TAACATTTCA AAGAGGAGAT 
GGAGGACTAA TGAGCAATGG GAGCCGTCCA GGTCTTCTAA ATGCTGGCGA TCCCAATTAC 
CCATGGCTTG CTGATTCTTG GCCAGCCACG AGTTTGCCAG TGAACAATAG CAATAGTGGC 
CCAAATGAAA TTGGAAATTT TGGGCGTGGA GATGTGCTGC CTCCGGTGCC AGGCCAAGGG 
GATAAAACAG CGACCATGCT CTCGGATGGA GCCATTTATA GCAGCATTGA CTTCACTACC 
AAAACCACTT ACAACAGTTC CAGCCAAATA ACACAGGCCA CCCCATATGC CACTACACAA 
ATCCTGCATT CAAACAGCAT CCACGAACTG GCAGTTGATC TTCCTGATCC ACAGTGGAAA 
AGCTCAGTTC AACAGAAGAC AGACCTCATG GGATTTGGTT ATTCGCTACC TGATCAGAAC 
AAGGGGAACA ACGCCTTACT TTACATCCCT GACTACCGAT TGGCTGAGGG ATTGTCTAAT 
AGAATGCCAC ACAACCAGTC ACAGGATTTC AGCACCACCA GCTCTCACAA CAGCTCAGAA 
AGGAGTGGCA GTCTCTCAGG TGGGAAAGGT GGAAAAAAGA AGAAAACTAA AAATTCTTCG 
AAAGCGCAGA AAAACAACGG ATCCACTTGG GCTAATGTCC CTCTACCTCC TCCTCCCGTC 
CAGCCCCTTC CTGGTACAGA GCTGGGCCAC TATGCTGCGG AACAAGAAAA TGGCTATGAC 
AGTGACAGCT GGTGCCCACC ATTACCGGTG CAAACATACT TGCATCAGGG TATGGAAGAT 
GAGCTGGAAG AAGACGAAGA TCGGGTCCCA ACACCTCCTG TTCGCGGCGT GGCCTCTTCA 
CCAGCTATCT CTTTTGGACA GCAGTCCACT GCCACTCTTA CTCCATCCCC ACGGGAAGAG 
ATGCAACCCA TGCTGCAAGC TCACTTGGAT GAGTTGACAA GGGCCTATCA GTTTGATATA 
GCAAAACAAA CATGGCACAT TCAAAGCAAT ACCCCACCTC CACAACCCCC AGCTCCGCCA 
TTAGGTTACG TGTCCGGAGC CTTGATTTCT GATTTGGAGA CAGATGTTCC AGATGAGGAT 
GCTGATGATG AAGAGGAACC ATTAGAAATT CCCAGGCCCC TCAGAGCACT AGACCAGACA 
CCTGGATCCA GTATGGACAA TCTAGACAGC TCTGTCACAG GAAAAGCCTT TAGCTCCTCT 
CAAAGGCAGC GGCCCACCAG CCCATTTTCT ACGGACAGTA ACACCAGTGC TGCCCAGAAT 
CAAAGCCAGA GGCCTCGGCC CACGAAAAAA CATAAGGGAG GACGGATGGA CCCACAGCCA 
GTGTTACCTC ATCGGAGGGA AGGGATGCCA GATGATCTTC CCCCACCACC AGATCCCCCA 
CCAGGTCAGG GTTTAAGGCA GCAGATAGGC CTGAGCCAAC ACTCTGGTAA CGTGGAAAAC 
TCAACAGAGA GAAAAGGAAG CTCTCTAGAG AGACAACAAG CAGCCAACTT AGAAGACACA 
AAGAGCTCAT TGGATTGTCC AGCTAAAACT GTTCTAGAGT GGCAGCGTCA AACCCAGGAC 
TGGATAAACT CTACAGAACG CCAAGAAGAA ACTCGGAAAG CCCCACACAA ACAAGGGGTT 
GGATCAGAGG AGTCCCTGGT GCCCTACAGC AAGCCCAGCT TCCCGTCTCC TGGCGGGCAC 
AGCTCATCAG GAACATCTTC CTCTAAAGGG TCCACTGGCC CTCGGAAGGC TGACGTGCTG 
AGAGGCAGCC ACCAGAGGAA TGCCAATGAC CTTCTCGACA TTGGATATGT GGGCTCAAAT 
AGTCAAGGAC AGTTTACAGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523107.1
CDS	236..4819
Translation	MARRQESICGRPWTWTPGLLMLIILGIHQGSGQGQGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKAMSTDEGTYVCIAENRVGKVEASATLTVRVRPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPINQTLAVDGTALLKCKATGEPLPVISWLKEGFTFLGRDPRATIQDQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDMNDLPGPPSKPQVTDVSKNSVTLSWQPGTPGVLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVVVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQASTVWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSHNSTSISVSWDPPPADHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVVIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGGRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPNYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTTYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSVQQKTDLMGFGYSLPDQNKGNNALLYIPDYRLAEGLSNRMPHNQSQDFSTTSSHNSSERSGSLSGGKGGKKKKTKNSSKAQKNNGSTWANVPLPPPPVQPLPGTELGHYAAEQENGYDSDSWCPPLPVQTYLHQGMEDELEEDEDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNTPPPQPPAPPLGYVSGALISDLETDVPDEDADDEEEPLEIPRPLRALDQTPGSSMDNLDSSVTGKAFSSSQRQRPTSPFSTDSNTSAAQNQSQRPRPTKKHKGGRMDPQPVLPHRREGMPDDLPPPPDPPPGQGLRQQIGLSQHSGNVENSTERKGSSLERQQAANLEDTKSSLDCPAKTVLEWQRQTQDWINSTERQEETRKAPHKQGVGSEESLVPYSKPSFPSPGGHSSSGTSSSKGSTGPRKADVLRGSHQRNANDLLDIGYVGSNSQGQFTE

Target ORF information:

RefSeq Version	XM_006523044.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523044.3

ORF Insert Sequence:

ATGGCCAGAA GACAAGAAAG TATCTGTGGA AGGCCGTGGA CGTGGACGCC AGGACTACTG 
ATGCTGATCA TCCTTGGCAT TCATCAGGGG AGTGGGCAAG GTCAAGGATC TCGTCTTCGT 
CAAGAAGACT TTCCCCCCAG GATCGTAGAA CACCCTTCTG ATGTCATCGT CTCCAAGGGA 
GAGCCCACCA CTCTGAACTG TAAAGCAGAG GGCCGACCCA CCCCCACCAT TGAATGGTAC 
AAGGATGGTG AGAGGGTGGA GACAGACAAG GATGATCCCA GGTCCCACAG AATGCTTCTG 
CCCAGCGGAT CTTTATTCTT TTTGCGAATT GTTCATGGGC GCAGAAGTAA ACCGGACGAA 
GGGAGTTACG TTTGTGTTGC AAGGAACTAT CTTGGTGAAG CAGTGAGTCG AAATGCATCT 
CTGGAAGTGG CATTATTGCG AGATGACTTC CGGCAAAACC CCACAGATGT GGTAGTCGCA 
GCTGGAGAGC CTGCAATCTT GGAGTGCCAG CCACCACGGG GACACCCAGA ACCAACCATC 
TACTGGAAAA AGGACAAAGT CCGAATTGAT GACAAGGAAG AGAGAATAAG TATCCGTGGT 
GGGAAGCTGA TGATCTCTAA TACTAGGAAA AGCGATGCTG GCATGTACAC CTGTGTGGGA 
ACCAATATGG TGGGAGAAAG GGACAGCGAC CCTGCAGAGC TCACTGTCTT TGAACGACCC 
ACATTTCTCA GGAGGCCAAT TAACCAGGTG GTGCTAGAGG AAGAAGCTGT AGAATTCCGT 
TGTCAGGTCC AAGGAGATCC CCAGCCAACG GTGAGGTGGA AAAAAGATGA TGCAGACTTG 
CCGAGAGGAA GGTATGATAT CAAAGATGAC TACACGCTGA GAATTAAAAA GGCCATGAGT 
ACTGATGAAG GTACCTATGT GTGTATTGCT GAGAATCGGG TGGGAAAAGT GGAAGCCTCT 
GCTACCCTCA CTGTCCGAGT TCGCCCTGTT GCTCCTCCAC AGTTTGTGGT TAGGCCAAGA 
GATCAGATCG TTGCTCAAGG CCGAACAGTG ACATTCCCCT GTGAAACTAA AGGAAACCCA 
CAGCCAGCTG TTTTTTGGCA GAAAGAAGGC AGCCAGAACC TACTTTTCCC GAATCAACCT 
CAGCAGCCCA ACAGCCGATG TTCAGTGTCG CCCACGGGGG ACCTCACCAT CACCAACATC 
CAGCGTTCAG ATGCGGGTTA CTACATCTGC CAGGCCCTAA CCGTGGCAGG AAGCATTTTA 
GCTAAAGCAC AGTTGGAAGT TACTGACGTT TTGACAGATA GACCTCCACC CATAATCTTG 
CAAGGACCAA TAAACCAAAC ACTTGCAGTA GACGGTACAG CATTGTTGAA GTGTAAAGCC 
ACTGGTGAGC CTCTGCCTGT AATTAGCTGG CTAAAGGAGG GCTTTACTTT TCTGGGGAGA 
GATCCAAGAG CCACGATCCA AGACCAAGGA ACACTGCAGA TTAAGAATTT ACGGATATCT 
GATACTGGCA CTTATACTTG TGTGGCTACA AGTTCCAGTG GAGAGACTTC CTGGAGTGCA 
GTGCTGGATG TAACAGAATC TGGAGCAACA ATCAGTAAAA ATTATGATAT GAATGACCTC 
CCGGGACCAC CATCCAAACC TCAGGTCACT GATGTTTCTA AGAACAGTGT CACCTTATCC 
TGGCAGCCAG GTACACCTGG CGTTCTTCCT GCAAGCGCGT ATATCATTGA GGCTTTCAGC 
CAATCGGTGA GCAATAGCTG GCAGACAGTG GCAAACCATG TTAAGACAAC TCTGTATACA 
GTAAGGGGGC TGAGGCCCAA CACAATCTAC TTGTTTATGG TCAGAGCGAT CAACCCACAA 
GGTCTCAGTG ATCCAAGTCC TATGTCGGAT CCTGTACGCA CACAAGATAT CAGCCCCCCA 
GCACAAGGAG TGGACCACAG ACAGGTGCAG AAGGAATTAG GTGATGTCGT TGTTCGTCTC 
CATAATCCAG TTGTCCTGAC ACCTACAACT GTTCAAGTCA CATGGACGGT GGACCGACAA 
CCCCAGTTTA TTCAGGGCTA CAGAGTGATG TACCGTCAGA CTTCGGGACT ACAAGCCTCA 
ACTGTGTGGC AGAATCTAGA CGCCAAAGTC CCGACTGAGA GGAGTGCTGT CCTTGTGAAT 
TTGAAAAAGG GGGTGACTTA TGAAATTAAA GTCCGGCCGT ATTTTAACGA GTTCCAAGGA 
ATGGACAGTG AATCGAAAAC AGTCCGAACC ACTGAGGAAG CCCCAAGTGC CCCTCCCCAG 
TCTGTCACTG TGCTGACAGT TGGAAGTCAC AACAGCACAA GCATCAGTGT TTCCTGGGAT 
CCTCCACCAG CCGACCACCA GAATGGAATT ATTCAGGAAT ATAAGATCTG GTGTCTGGGA 
AACGAAACGC GATTCCATAT CAATAAAACG GTGGATGCAG CCATTCGCTC TGTAGTAATA 
GGTGGCTTGT TCCCTGGAAT TCAGTACCGG GTAGAAGTGG CAGCTAGCAC AAGTGCAGGG 
GTTGGAGTAA AAAGTGAACC ACAGCCGATA ATAATTGGGG GACGTAACGA AGTTGTCATT 
ACTGAAAACA ATAACAGCAT CACTGAGCAA ATCACGGATG TCGTGAAGCA ACCGGCATTT 
ATAGCTGGCA TTGGTGGTGC CTGCTGGGTA ATTCTGATGG GTTTTAGCAT CTGGTTGTAC 
TGGAGAAGAA AGAAGAGAAA GGGACTCAGC AATTATGCTG TAACATTTCA AAGAGGAGAT 
GGAGGACTAA TGAGCAATGG GAGCCGTCCA GGTCTTCTAA ATGCTGGCGA TCCCAATTAC 
CCATGGCTTG CTGATTCTTG GCCAGCCACG AGTTTGCCAG TGAACAATAG CAATAGTGGC 
CCAAATGAAA TTGGAAATTT TGGGCGTGGA GATGTGCTGC CTCCGGTGCC AGGCCAAGGG 
GATAAAACAG CGACCATGCT CTCGGATGGA GCCATTTATA GCAGCATTGA CTTCACTACC 
AAAACCACTT ACAACAGTTC CAGCCAAATA ACACAGGCCA CCCCATATGC CACTACACAA 
ATCCTGCATT CAAACAGCAT CCACGAACTG GCAGTTGATC TTCCTGATCC ACAGTGGAAA 
AGCTCAGTTC AACAGAAGAC AGACCTCATG GGATTTGGTT ATTCGCTACC TGATCAGAAC 
AAGGGGAACA ACGCCTTACT TTACATCCCT GACTACCGAT TGGCTGAGGG ATTGTCTAAT 
AGAATGCCAC ACAACCAGTC ACAGGATTTC AGCACCACCA GCTCTCACAA CAGCTCAGAA 
AGGAGTGGCA GTCTCTCAGG TGGGAAAGGT GGAAAAAAGA AGAAAACTAA AAATTCTTCG 
AAAGCGCAGA AAAACAACGG ATCCACTTGG GCTAATGTCC CTCTACCTCC TCCTCCCGTC 
CAGCCCCTTC CTGGTACAGA GCTGGGCCAC TATGCTGCGG AACAAGAAAA TGGCTATGAC 
AGTGACAGCT GGTGCCCACC ATTACCGGTG CAAACATACT TGCATCAGGG TATGGAAGAT 
GAGCTGGAAG AAGACGAAGA TCGGGTCCCA ACACCTCCTG TTCGCGGCGT GGCCTCTTCA 
CCAGCTATCT CTTTTGGACA GCAGTCCACT GCCACTCTTA CTCCATCCCC ACGGGAAGAG 
ATGCAACCCA TGCTGCAAGC TCACTTGGAT GAGTTGACAA GGGCCTATCA GTTTGATATA 
GCAAAACAAA CATGGCACAT TCAAAGCAAT ACCCCACCTC CACAACCCCC AGCTCCGCCA 
TTAGGTTACG TGTCCGGAGC CTTGATTTCT GATTTGGAGA CAGATGTTCC AGATGAGGAT 
GCTGATGATG AAGAGGAACC ATTAGAAATT CCCAGGCCCC TCAGAGCACT AGACCAGACA 
CCTGGATCCA GTATGGACAA TCTAGACAGC TCTGTCACAG GAAAAGCCTT TAGCTCCTCT 
CAAAGGCAGC GGCCCACCAG CCCATTTTCT ACGGACAGTA ACACCAGTGC TGCCCAGAAT 
CAAAGCCAGA GGCCTCGGCC CACGAAAAAA CATAAGGGAG GACGGATGGA CCCACAGCCA 
GTGTTACCTC ATCGGAGGGA AGGGATGCCA GATGATCTTC CCCCACCACC AGATCCCCCA 
CCAGGTCAGG GTTTAAGGCA GCAGATAGGC CTGAGCCAAC ACTCTGGTAA CGTGGAAAAC 
TCAACAGAGA GAAAAGGAAG CTCTCTAGAG AGACAACAAG CAGCCAACTT AGAAGACACA 
AAGAGCTCAT TGGATTGTCC AGCTAAAACT GTTCTAGAGT GGCAGCGTCA AACCCAGGAC 
TGGATAAACT CTACAGAACG CCAAGAAGAA ACTCGGAAAG CCCCACACAA ACAAGGGGTT 
GGATCAGAGG AGTCCCTGGT GCCCTACAGC AAGCCCAGCT TCCCGTCTCC TGGCGGGCAC 
AGCTCATCAG GAACATCTTC CTCTAAAGGG TCCACTGGCC CTCGGAAGGC TGACGTGCTG 
AGAGGCAGCC ACCAGAGGAA TGCCAATGAC CTTCTCGACA TTGGATATGT GGGCTCAAAT 
AGTCAAGGAC AGTTTACAGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo2 ( XM_006523045.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523108.1 Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X12, mRNA.

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CloneID	OMu52765
Clone ID Related Accession (Same CDS sequence)	XM_006523045.4 , XM_006523045.3
Accession Version	XM_006523045.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	roundabout homolog 2 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006523045.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT 
CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG 
GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC 
CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA 
GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT 
ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG 
GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG 
CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT 
AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT 
TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC 
GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC 
CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT 
ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA 
CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA 
TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA 
GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT 
CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT 
GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG 
CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG 
GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC 
ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG 
GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT 
GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA 
GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT 
GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG 
GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT 
GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT 
CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG 
CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG 
CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT 
TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG 
GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT 
GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT 
GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGCCT TACTTTACAT CCCTGACTAC 
CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC 
ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA 
AAGAAGAAAA CTAAAAATTC TTCGAAAGCG CAGAAAAACA ACGGATCCAC TTGGGCTAAT 
GTCCCTCTAC CTCCTCCTCC CGTCCAGCCC CTTCCTGGTA CAGAGCTGGG CCACTATGCT 
GCGGAACAAG AAAATGGCTA TGACAGTGAC AGCTGGTGCC CACCATTACC GGTGCAAACA 
TACTTGCATC AGGGTATGGA AGATGAGCTG GAAGAAGACG AAGATCGGGT CCCAACACCT 
CCTGTTCGCG GCGTGGCCTC TTCACCAGCT ATCTCTTTTG GACAGCAGTC CACTGCCACT 
CTTACTCCAT CCCCACGGGA AGAGATGCAA CCCATGCTGC AAGCTCACTT GGATGAGTTG 
ACAAGGGCCT ATCAGTTTGA TATAGCAAAA CAAACATGGC ACATTCAAAG CAATACCCCA 
CCTCCACAAC CCCCAGCTCC GCCATTAGGT TACGTGTCCG GAGCCTTGAT TTCTGATTTG 
GAGACAGATG TTCCAGATGA GGATGCTGAT GATGAAGAGG AACCATTAGA AATTCCCAGG 
CCCCTCAGAG CACTAGACCA GACACCTGGA TCCAGTATGG ACAATCTAGA CAGCTCTGTC 
ACAGGTTCAA TGGTAAATGG ATGGGGCTCT GCATCTGATG AGGATCGTAA CTTTTCTAGT 
CATAGATCTA GTGTAGGTAG CTCCTCAGAT GGCTCCATCT TTGCCAGCGG CAGCTTTGCA 
AAAGCATTGG TGGCAGCAGC AGACAAAGCT GGCTTTAGGC TGGATGGAAC CAGTCTTACG 
AGAACAGGAA AAGCCTTTAG CTCCTCTCAA AGGCAGCGGC CCACCAGCCC ATTTTCTACG 
GACAGTAACA CCAGTGCTGC CCAGAATCAA AGCCAGAGGC CTCGGCCCAC GAAAAAACAT 
AAGGGAGGAC GGATGGACCC ACAGCCAGTG TTACCTCATC GGAGGGAAGG GATGCCAGAT 
GAGGAGTCCC TGGTGCCCTA CAGCAAGCCC AGCTTCCCGT CTCCTGGCGG GCACAGCTCA 
TCAGGAACAT CTTCCTCTAA AGGGTCCACT GGCCCTCGGA AGGCTGACGT GCTGAGAGGC 
AGCCACCAGA GGAATGCCAA TGACCTTCTC GACATTGGAT ATGTGGGCTC AAATAGTCAA 
GGACAGTTTA CAGGTGAATT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523108.1
CDS	337..4860
Translation	MLSSEKVDKITRLELLFVLMLFFSGPTLGKLTRTPWKGKPWKGGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKAMSTDEGTYVCIAENRVGKVEASATLTVRVRPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPINQTLAVDGTALLKCKATGEPLPVISWLKEGFTFLGRDPRATIQDQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDMNDLPGPPSKPQVTDVSKNSVTLSWQPGTPGVLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVVVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQASTVWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSHNSTSISVSWDPPPADHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVVIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGGRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPNYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTTYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSVQQKTDLMGFGYSLPDQNKGNNALLYIPDYRLAEGLSNRMPHNQSQDFSTTSSHNSSERSGSLSGGKGGKKKKTKNSSKAQKNNGSTWANVPLPPPPVQPLPGTELGHYAAEQENGYDSDSWCPPLPVQTYLHQGMEDELEEDEDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNTPPPQPPAPPLGYVSGALISDLETDVPDEDADDEEEPLEIPRPLRALDQTPGSSMDNLDSSVTGSMVNGWGSASDEDRNFSSHRSSVGSSSDGSIFASGSFAKALVAAADKAGFRLDGTSLTRTGKAFSSSQRQRPTSPFSTDSNTSAAQNQSQRPRPTKKHKGGRMDPQPVLPHRREGMPDEESLVPYSKPSFPSPGGHSSSGTSSSKGSTGPRKADVLRGSHQRNANDLLDIGYVGSNSQGQFTGEL

Target ORF information:

RefSeq Version	XM_006523045.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523045.3

ORF Insert Sequence:

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT 
CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG 
GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC 
CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA 
GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT 
ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG 
GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG 
CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT 
AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT 
TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC 
GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC 
CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT 
ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA 
CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA 
TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA 
GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT 
CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT 
GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG 
CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG 
GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC 
ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG 
GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT 
GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA 
GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT 
GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG 
GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT 
GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT 
CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG 
CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG 
CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT 
TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG 
GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT 
GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT 
GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGCCT TACTTTACAT CCCTGACTAC 
CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC 
ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA 
AAGAAGAAAA CTAAAAATTC TTCGAAAGCG CAGAAAAACA ACGGATCCAC TTGGGCTAAT 
GTCCCTCTAC CTCCTCCTCC CGTCCAGCCC CTTCCTGGTA CAGAGCTGGG CCACTATGCT 
GCGGAACAAG AAAATGGCTA TGACAGTGAC AGCTGGTGCC CACCATTACC GGTGCAAACA 
TACTTGCATC AGGGTATGGA AGATGAGCTG GAAGAAGACG AAGATCGGGT CCCAACACCT 
CCTGTTCGCG GCGTGGCCTC TTCACCAGCT ATCTCTTTTG GACAGCAGTC CACTGCCACT 
CTTACTCCAT CCCCACGGGA AGAGATGCAA CCCATGCTGC AAGCTCACTT GGATGAGTTG 
ACAAGGGCCT ATCAGTTTGA TATAGCAAAA CAAACATGGC ACATTCAAAG CAATACCCCA 
CCTCCACAAC CCCCAGCTCC GCCATTAGGT TACGTGTCCG GAGCCTTGAT TTCTGATTTG 
GAGACAGATG TTCCAGATGA GGATGCTGAT GATGAAGAGG AACCATTAGA AATTCCCAGG 
CCCCTCAGAG CACTAGACCA GACACCTGGA TCCAGTATGG ACAATCTAGA CAGCTCTGTC 
ACAGGTTCAA TGGTAAATGG ATGGGGCTCT GCATCTGATG AGGATCGTAA CTTTTCTAGT 
CATAGATCTA GTGTAGGTAG CTCCTCAGAT GGCTCCATCT TTGCCAGCGG CAGCTTTGCA 
AAAGCATTGG TGGCAGCAGC AGACAAAGCT GGCTTTAGGC TGGATGGAAC CAGTCTTACG 
AGAACAGGAA AAGCCTTTAG CTCCTCTCAA AGGCAGCGGC CCACCAGCCC ATTTTCTACG 
GACAGTAACA CCAGTGCTGC CCAGAATCAA AGCCAGAGGC CTCGGCCCAC GAAAAAACAT 
AAGGGAGGAC GGATGGACCC ACAGCCAGTG TTACCTCATC GGAGGGAAGG GATGCCAGAT 
GAGGAGTCCC TGGTGCCCTA CAGCAAGCCC AGCTTCCCGT CTCCTGGCGG GCACAGCTCA 
TCAGGAACAT CTTCCTCTAA AGGGTCCACT GGCCCTCGGA AGGCTGACGT GCTGAGAGGC 
AGCCACCAGA GGAATGCCAA TGACCTTCTC GACATTGGAT ATGTGGGCTC AAATAGTCAA 
GGACAGTTTA CAGGTGAATT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo2 ( XM_006523046.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523109.1 Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OMu52766
Clone ID Related Accession (Same CDS sequence)	XM_006523046.3 , XM_006523046.4
Accession Version	XM_006523046.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4488bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	roundabout homolog 2 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006523046.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT 
CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG 
GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC 
CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA 
GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT 
ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG 
GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG 
CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT 
AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT 
TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC 
GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC 
CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT 
ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA 
CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA 
TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA 
GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT 
CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT 
GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG 
CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG 
GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC 
ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG 
GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT 
GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA 
GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT 
GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG 
GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT 
GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT 
CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG 
CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG 
CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT 
TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG 
GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT 
GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT 
GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGGTG GGAAAGGTGG AAAAAAGAAG 
AAAACTAAAA ATTCTTCGAA AGCGCAGAAA AACAACGGAT CCACTTGGGC TAATGTCCCT 
CTACCTCCTC CTCCCGTCCA GCCCCTTCCT GGTACAGAGC TGGGCCACTA TGCTGCGGAA 
CAAGAAAATG GCTATGACAG TGACAGCTGG TGCCCACCAT TACCGGTGCA AACATACTTG 
CATCAGGGTA TGGAAGATGA GCTGGAAGAA GACGAAGATC GGGTCCCAAC ACCTCCTGTT 
CGCGGCGTGG CCTCTTCACC AGCTATCTCT TTTGGACAGC AGTCCACTGC CACTCTTACT 
CCATCCCCAC GGGAAGAGAT GCAACCCATG CTGCAAGCTC ACTTGGATGA GTTGACAAGG 
GCCTATCAGT TTGATATAGC AAAACAAACA TGGCACATTC AAAGCAATAC CCCACCTCCA 
CAACCCCCAG CTCCGCCATT AGGTTACGTG TCCGGAGCCT TGATTTCTGA TTTGGAGACA 
GATGTTCCAG ATGAGGATGC TGATGATGAA GAGGAACCAT TAGAAATTCC CAGGCCCCTC 
AGAGCACTAG ACCAGACACC TGGATCCAGT ATGGACAATC TAGACAGCTC TGTCACAGGA 
AAAGCCTTTA GCTCCTCTCA AAGGCAGCGG CCCACCAGCC CATTTTCTAC GGACAGTAAC 
ACCAGTGCTG CCCAGAATCA AAGCCAGAGG CCTCGGCCCA CGAAAAAACA TAAGGGAGGA 
CGGATGGACC CACAGCCAGT GTTACCTCAT CGGAGGGAAG GGATGCCAGA TGATCTTCCC 
CCACCACCAG ATCCCCCACC AGGTCAGGGT TTAAGGCAGC AGATAGGCCT GAGCCAACAC 
TCTGGTAACG TGGAAAACTC AACAGAGAGA AAAGGAAGCT CTCTAGAGAG ACAACAAGCA 
GCCAACTTAG AAGACACAAA GAGCTCATTG GATTGTCCAG CTAAAACTGT TCTAGAGTGG 
CAGCGTCAAA CCCAGGACTG GATAAACTCT ACAGAACGCC AAGAAGAAAC TCGGAAAGCC 
CCACACAAAC AAGGGGTTGG ATCAGAGGAG TCCCTGGTGC CCTACAGCAA GCCCAGCTTC 
CCGTCTCCTG GCGGGCACAG CTCATCAGGA ACATCTTCCT CTAAAGGGTC CACTGGCCCT 
CGGAAGGCTG ACGTGCTGAG AGGCAGCCAC CAGAGGAATG CCAATGACCT TCTCGACATT 
GGATATGTGG GCTCAAATAG TCAAGGACAG TTTACAGGTG AATTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523109.1
CDS	337..4824
Translation	MLSSEKVDKITRLELLFVLMLFFSGPTLGKLTRTPWKGKPWKGGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKAMSTDEGTYVCIAENRVGKVEASATLTVRVRPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPINQTLAVDGTALLKCKATGEPLPVISWLKEGFTFLGRDPRATIQDQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDMNDLPGPPSKPQVTDVSKNSVTLSWQPGTPGVLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVVVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQASTVWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSHNSTSISVSWDPPPADHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVVIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGGRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPNYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTTYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSVQQKTDLMGFGYSLPDQNKGNNGGKGGKKKKTKNSSKAQKNNGSTWANVPLPPPPVQPLPGTELGHYAAEQENGYDSDSWCPPLPVQTYLHQGMEDELEEDEDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNTPPPQPPAPPLGYVSGALISDLETDVPDEDADDEEEPLEIPRPLRALDQTPGSSMDNLDSSVTGKAFSSSQRQRPTSPFSTDSNTSAAQNQSQRPRPTKKHKGGRMDPQPVLPHRREGMPDDLPPPPDPPPGQGLRQQIGLSQHSGNVENSTERKGSSLERQQAANLEDTKSSLDCPAKTVLEWQRQTQDWINSTERQEETRKAPHKQGVGSEESLVPYSKPSFPSPGGHSSSGTSSSKGSTGPRKADVLRGSHQRNANDLLDIGYVGSNSQGQFTGEL

Target ORF information:

RefSeq Version	XM_006523046.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523046.3

ORF Insert Sequence:

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT 
CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG 
GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC 
CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA 
GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT 
ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG 
GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG 
CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT 
AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT 
TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC 
GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC 
CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT 
ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA 
CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA 
TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA 
GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT 
CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT 
GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG 
CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG 
GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC 
ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG 
GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT 
GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA 
GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT 
GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG 
GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT 
GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT 
CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG 
CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG 
CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT 
TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG 
GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT 
GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT 
GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGGTG GGAAAGGTGG AAAAAAGAAG 
AAAACTAAAA ATTCTTCGAA AGCGCAGAAA AACAACGGAT CCACTTGGGC TAATGTCCCT 
CTACCTCCTC CTCCCGTCCA GCCCCTTCCT GGTACAGAGC TGGGCCACTA TGCTGCGGAA 
CAAGAAAATG GCTATGACAG TGACAGCTGG TGCCCACCAT TACCGGTGCA AACATACTTG 
CATCAGGGTA TGGAAGATGA GCTGGAAGAA GACGAAGATC GGGTCCCAAC ACCTCCTGTT 
CGCGGCGTGG CCTCTTCACC AGCTATCTCT TTTGGACAGC AGTCCACTGC CACTCTTACT 
CCATCCCCAC GGGAAGAGAT GCAACCCATG CTGCAAGCTC ACTTGGATGA GTTGACAAGG 
GCCTATCAGT TTGATATAGC AAAACAAACA TGGCACATTC AAAGCAATAC CCCACCTCCA 
CAACCCCCAG CTCCGCCATT AGGTTACGTG TCCGGAGCCT TGATTTCTGA TTTGGAGACA 
GATGTTCCAG ATGAGGATGC TGATGATGAA GAGGAACCAT TAGAAATTCC CAGGCCCCTC 
AGAGCACTAG ACCAGACACC TGGATCCAGT ATGGACAATC TAGACAGCTC TGTCACAGGA 
AAAGCCTTTA GCTCCTCTCA AAGGCAGCGG CCCACCAGCC CATTTTCTAC GGACAGTAAC 
ACCAGTGCTG CCCAGAATCA AAGCCAGAGG CCTCGGCCCA CGAAAAAACA TAAGGGAGGA 
CGGATGGACC CACAGCCAGT GTTACCTCAT CGGAGGGAAG GGATGCCAGA TGATCTTCCC 
CCACCACCAG ATCCCCCACC AGGTCAGGGT TTAAGGCAGC AGATAGGCCT GAGCCAACAC 
TCTGGTAACG TGGAAAACTC AACAGAGAGA AAAGGAAGCT CTCTAGAGAG ACAACAAGCA 
GCCAACTTAG AAGACACAAA GAGCTCATTG GATTGTCCAG CTAAAACTGT TCTAGAGTGG 
CAGCGTCAAA CCCAGGACTG GATAAACTCT ACAGAACGCC AAGAAGAAAC TCGGAAAGCC 
CCACACAAAC AAGGGGTTGG ATCAGAGGAG TCCCTGGTGC CCTACAGCAA GCCCAGCTTC 
CCGTCTCCTG GCGGGCACAG CTCATCAGGA ACATCTTCCT CTAAAGGGTC CACTGGCCCT 
CGGAAGGCTG ACGTGCTGAG AGGCAGCCAC CAGAGGAATG CCAATGACCT TCTCGACATT 
GGATATGTGG GCTCAAATAG TCAAGGACAG TTTACAGGTG AATTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo2 ( XM_006523047.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523110.1 Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OMu52767
Clone ID Related Accession (Same CDS sequence)	NM_001358491.1 , XM_006523047.3
Accession Version	XM_006523047.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4482bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	roundabout homolog 2 isoform X14
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006523047.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT 
CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG 
GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC 
CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA 
GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT 
ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG 
GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG 
CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT 
AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT 
TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC 
GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC 
CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT 
ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA 
CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA 
TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA 
GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT 
CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT 
GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG 
CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG 
GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC 
ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG 
GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT 
GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA 
GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT 
GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG 
GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT 
GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT 
CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG 
CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG 
CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT 
TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG 
GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT 
GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT 
GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGGTG GGAAAGGTGG AAAAAAGAAG 
AAAACTAAAA ATTCTTCGAA AGCGCAGAAA AACAACGGAT CCACTTGGGC TAATGTCCCT 
CTACCTCCTC CTCCCGTCCA GCCCCTTCCT GGTACAGAGC TGGGCCACTA TGCTGCGGAA 
CAAGAAAATG GCTATGACAG TGACAGCTGG TGCCCACCAT TACCGGTGCA AACATACTTG 
CATCAGGGTA TGGAAGATGA GCTGGAAGAA GACGAAGATC GGGTCCCAAC ACCTCCTGTT 
CGCGGCGTGG CCTCTTCACC AGCTATCTCT TTTGGACAGC AGTCCACTGC CACTCTTACT 
CCATCCCCAC GGGAAGAGAT GCAACCCATG CTGCAAGCTC ACTTGGATGA GTTGACAAGG 
GCCTATCAGT TTGATATAGC AAAACAAACA TGGCACATTC AAAGCAATAC CCCACCTCCA 
CAACCCCCAG CTCCGCCATT AGGTTACGTG TCCGGAGCCT TGATTTCTGA TTTGGAGACA 
GATGTTCCAG ATGAGGATGC TGATGATGAA GAGGAACCAT TAGAAATTCC CAGGCCCCTC 
AGAGCACTAG ACCAGACACC TGGATCCAGT ATGGACAATC TAGACAGCTC TGTCACAGGA 
AAAGCCTTTA GCTCCTCTCA AAGGCAGCGG CCCACCAGCC CATTTTCTAC GGACAGTAAC 
ACCAGTGCTG CCCAGAATCA AAGCCAGAGG CCTCGGCCCA CGAAAAAACA TAAGGGAGGA 
CGGATGGACC CACAGCCAGT GTTACCTCAT CGGAGGGAAG GGATGCCAGA TGATCTTCCC 
CCACCACCAG ATCCCCCACC AGGTCAGGGT TTAAGGCAGC AGATAGGCCT GAGCCAACAC 
TCTGGTAACG TGGAAAACTC AACAGAGAGA AAAGGAAGCT CTCTAGAGAG ACAACAAGCA 
GCCAACTTAG AAGACACAAA GAGCTCATTG GATTGTCCAG CTAAAACTGT TCTAGAGTGG 
CAGCGTCAAA CCCAGGACTG GATAAACTCT ACAGAACGCC AAGAAGAAAC TCGGAAAGCC 
CCACACAAAC AAGGGGTTGG ATCAGAGGAG TCCCTGGTGC CCTACAGCAA GCCCAGCTTC 
CCGTCTCCTG GCGGGCACAG CTCATCAGGA ACATCTTCCT CTAAAGGGTC CACTGGCCCT 
CGGAAGGCTG ACGTGCTGAG AGGCAGCCAC CAGAGGAATG CCAATGACCT TCTCGACATT 
GGATATGTGG GCTCAAATAG TCAAGGACAG TTTACAGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523110.1
CDS	337..4818
Translation	MLSSEKVDKITRLELLFVLMLFFSGPTLGKLTRTPWKGKPWKGGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKAMSTDEGTYVCIAENRVGKVEASATLTVRVRPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPINQTLAVDGTALLKCKATGEPLPVISWLKEGFTFLGRDPRATIQDQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDMNDLPGPPSKPQVTDVSKNSVTLSWQPGTPGVLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVVVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQASTVWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSHNSTSISVSWDPPPADHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVVIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGGRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPNYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTTYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSVQQKTDLMGFGYSLPDQNKGNNGGKGGKKKKTKNSSKAQKNNGSTWANVPLPPPPVQPLPGTELGHYAAEQENGYDSDSWCPPLPVQTYLHQGMEDELEEDEDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNTPPPQPPAPPLGYVSGALISDLETDVPDEDADDEEEPLEIPRPLRALDQTPGSSMDNLDSSVTGKAFSSSQRQRPTSPFSTDSNTSAAQNQSQRPRPTKKHKGGRMDPQPVLPHRREGMPDDLPPPPDPPPGQGLRQQIGLSQHSGNVENSTERKGSSLERQQAANLEDTKSSLDCPAKTVLEWQRQTQDWINSTERQEETRKAPHKQGVGSEESLVPYSKPSFPSPGGHSSSGTSSSKGSTGPRKADVLRGSHQRNANDLLDIGYVGSNSQGQFTE

Target ORF information:

RefSeq Version	XM_006523047.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523047.3

ORF Insert Sequence:

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT 
CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG 
GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC 
CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA 
GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT 
ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG 
GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG 
CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT 
AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT 
TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC 
GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC 
CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT 
ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA 
CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA 
TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA 
GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT 
CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT 
GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG 
CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG 
GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC 
ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG 
GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT 
GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA 
GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT 
GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG 
GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT 
GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT 
CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG 
CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG 
CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT 
TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG 
GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT 
GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT 
GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGGTG GGAAAGGTGG AAAAAAGAAG 
AAAACTAAAA ATTCTTCGAA AGCGCAGAAA AACAACGGAT CCACTTGGGC TAATGTCCCT 
CTACCTCCTC CTCCCGTCCA GCCCCTTCCT GGTACAGAGC TGGGCCACTA TGCTGCGGAA 
CAAGAAAATG GCTATGACAG TGACAGCTGG TGCCCACCAT TACCGGTGCA AACATACTTG 
CATCAGGGTA TGGAAGATGA GCTGGAAGAA GACGAAGATC GGGTCCCAAC ACCTCCTGTT 
CGCGGCGTGG CCTCTTCACC AGCTATCTCT TTTGGACAGC AGTCCACTGC CACTCTTACT 
CCATCCCCAC GGGAAGAGAT GCAACCCATG CTGCAAGCTC ACTTGGATGA GTTGACAAGG 
GCCTATCAGT TTGATATAGC AAAACAAACA TGGCACATTC AAAGCAATAC CCCACCTCCA 
CAACCCCCAG CTCCGCCATT AGGTTACGTG TCCGGAGCCT TGATTTCTGA TTTGGAGACA 
GATGTTCCAG ATGAGGATGC TGATGATGAA GAGGAACCAT TAGAAATTCC CAGGCCCCTC 
AGAGCACTAG ACCAGACACC TGGATCCAGT ATGGACAATC TAGACAGCTC TGTCACAGGA 
AAAGCCTTTA GCTCCTCTCA AAGGCAGCGG CCCACCAGCC CATTTTCTAC GGACAGTAAC 
ACCAGTGCTG CCCAGAATCA AAGCCAGAGG CCTCGGCCCA CGAAAAAACA TAAGGGAGGA 
CGGATGGACC CACAGCCAGT GTTACCTCAT CGGAGGGAAG GGATGCCAGA TGATCTTCCC 
CCACCACCAG ATCCCCCACC AGGTCAGGGT TTAAGGCAGC AGATAGGCCT GAGCCAACAC 
TCTGGTAACG TGGAAAACTC AACAGAGAGA AAAGGAAGCT CTCTAGAGAG ACAACAAGCA 
GCCAACTTAG AAGACACAAA GAGCTCATTG GATTGTCCAG CTAAAACTGT TCTAGAGTGG 
CAGCGTCAAA CCCAGGACTG GATAAACTCT ACAGAACGCC AAGAAGAAAC TCGGAAAGCC 
CCACACAAAC AAGGGGTTGG ATCAGAGGAG TCCCTGGTGC CCTACAGCAA GCCCAGCTTC 
CCGTCTCCTG GCGGGCACAG CTCATCAGGA ACATCTTCCT CTAAAGGGTC CACTGGCCCT 
CGGAAGGCTG ACGTGCTGAG AGGCAGCCAC CAGAGGAATG CCAATGACCT TCTCGACATT 
GGATATGTGG GCTCAAATAG TCAAGGACAG TTTACAGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo2 ( XM_006523048.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523111.1 Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X17, mRNA.

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CloneID	OMu52768
Clone ID Related Accession (Same CDS sequence)	XM_006523048.4 , XM_006523048.3
Accession Version	XM_006523048.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4335bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	roundabout homolog 2 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006523048.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT 
CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG 
GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC 
CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA 
GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT 
ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG 
GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG 
CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT 
AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT 
TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC 
GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC 
CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT 
ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA 
CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA 
TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA 
GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT 
CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT 
GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG 
CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG 
GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC 
ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG 
GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT 
GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA 
GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT 
GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG 
GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT 
GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT 
CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG 
CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG 
CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT 
TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG 
GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT 
GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT 
GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGCCT TACTTTACAT CCCTGACTAC 
CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC 
ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA 
AAGAAGAAAA CTAAAAATTC TTCGAAAGCG CAGAAAAACA ACGGATCCAC TTGGGCTAAT 
GTCCCTCTAC CTCCTCCTCC CGTCCAGCCC CTTCCTGGTA CAGAGCTGGG CCACTATGCT 
GCGGAACAAG AAAATGGCTA TGACAGTGAC AGCTGGTGCC CACCATTACC GGTGCAAACA 
TACTTGCATC AGGGTATGGA AGATGAGCTG GAAGAAGACG AAGATCGGGT CCCAACACCT 
CCTGTTCGCG GCGTGGCCTC TTCACCAGCT ATCTCTTTTG GACAGCAGTC CACTGCCACT 
CTTACTCCAT CCCCACGGGA AGAGATGCAA CCCATGCTGC AAGCTCACTT GGATGAGTTG 
ACAAGGGCCT ATCAGTTTGA TATAGCAAAA CAAACATGGC ACATTCAAAG CAATACCCCA 
CCTCCACAAC CCCCAGCTCC GCCATTAGGT TACGTGTCCG GAGCCTTGAT TTCTGATTTG 
GAGACAGATG TTCCAGATGA GGATGCTGAT GATGAAGAGG AACCATTAGA AATTCCCAGG 
CCCCTCAGAG CACTAGACCA GACACCTGGA TCCAGTATGG ACAATCTAGA CAGCTCTGTC 
ACAGGAAAAG CCTTTAGCTC CTCTCAAAGG CAGCGGCCCA CCAGCCCATT TTCTACGGAC 
AGTAACACCA GTGCTGCCCA GAATCAAAGC CAGAGGCCTC GGCCCACGAA AAAACATAAG 
GGAGGACGGA TGGACCCACA GCCAGTGTTA CCTCATCGGA GGGAAGGGAT GCCAGATGAG 
GAGTCCCTGG TGCCCTACAG CAAGCCCAGC TTCCCGTCTC CTGGCGGGCA CAGCTCATCA 
GGAACATCTT CCTCTAAAGG GTCCACTGGC CCTCGGAAGG CTGACGTGCT GAGAGGCAGC 
CACCAGAGGA ATGCCAATGA CCTTCTCGAC ATTGGATATG TGGGCTCAAA TAGTCAAGGA 
CAGTTTACAG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523111.1
CDS	337..4671
Translation	MLSSEKVDKITRLELLFVLMLFFSGPTLGKLTRTPWKGKPWKGGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKAMSTDEGTYVCIAENRVGKVEASATLTVRVRPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPINQTLAVDGTALLKCKATGEPLPVISWLKEGFTFLGRDPRATIQDQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDMNDLPGPPSKPQVTDVSKNSVTLSWQPGTPGVLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVVVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQASTVWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSHNSTSISVSWDPPPADHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVVIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGGRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPNYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTTYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSVQQKTDLMGFGYSLPDQNKGNNALLYIPDYRLAEGLSNRMPHNQSQDFSTTSSHNSSERSGSLSGGKGGKKKKTKNSSKAQKNNGSTWANVPLPPPPVQPLPGTELGHYAAEQENGYDSDSWCPPLPVQTYLHQGMEDELEEDEDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNTPPPQPPAPPLGYVSGALISDLETDVPDEDADDEEEPLEIPRPLRALDQTPGSSMDNLDSSVTGKAFSSSQRQRPTSPFSTDSNTSAAQNQSQRPRPTKKHKGGRMDPQPVLPHRREGMPDEESLVPYSKPSFPSPGGHSSSGTSSSKGSTGPRKADVLRGSHQRNANDLLDIGYVGSNSQGQFTE

Target ORF information:

RefSeq Version	XM_006523048.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523048.3

ORF Insert Sequence:

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT 
CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG 
GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC 
CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA 
GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT 
ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG 
GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG 
CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT 
AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT 
TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC 
GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC 
CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT 
ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA 
CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA 
TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA 
GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT 
CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT 
GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG 
CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG 
GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC 
ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG 
GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT 
GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA 
GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT 
GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG 
GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT 
GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT 
CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG 
CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG 
CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT 
TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG 
GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT 
GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT 
GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGCCT TACTTTACAT CCCTGACTAC 
CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCACAGGA TTTCAGCACC 
ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA 
AAGAAGAAAA CTAAAAATTC TTCGAAAGCG CAGAAAAACA ACGGATCCAC TTGGGCTAAT 
GTCCCTCTAC CTCCTCCTCC CGTCCAGCCC CTTCCTGGTA CAGAGCTGGG CCACTATGCT 
GCGGAACAAG AAAATGGCTA TGACAGTGAC AGCTGGTGCC CACCATTACC GGTGCAAACA 
TACTTGCATC AGGGTATGGA AGATGAGCTG GAAGAAGACG AAGATCGGGT CCCAACACCT 
CCTGTTCGCG GCGTGGCCTC TTCACCAGCT ATCTCTTTTG GACAGCAGTC CACTGCCACT 
CTTACTCCAT CCCCACGGGA AGAGATGCAA CCCATGCTGC AAGCTCACTT GGATGAGTTG 
ACAAGGGCCT ATCAGTTTGA TATAGCAAAA CAAACATGGC ACATTCAAAG CAATACCCCA 
CCTCCACAAC CCCCAGCTCC GCCATTAGGT TACGTGTCCG GAGCCTTGAT TTCTGATTTG 
GAGACAGATG TTCCAGATGA GGATGCTGAT GATGAAGAGG AACCATTAGA AATTCCCAGG 
CCCCTCAGAG CACTAGACCA GACACCTGGA TCCAGTATGG ACAATCTAGA CAGCTCTGTC 
ACAGGAAAAG CCTTTAGCTC CTCTCAAAGG CAGCGGCCCA CCAGCCCATT TTCTACGGAC 
AGTAACACCA GTGCTGCCCA GAATCAAAGC CAGAGGCCTC GGCCCACGAA AAAACATAAG 
GGAGGACGGA TGGACCCACA GCCAGTGTTA CCTCATCGGA GGGAAGGGAT GCCAGATGAG 
GAGTCCCTGG TGCCCTACAG CAAGCCCAGC TTCCCGTCTC CTGGCGGGCA CAGCTCATCA 
GGAACATCTT CCTCTAAAGG GTCCACTGGC CCTCGGAAGG CTGACGTGCT GAGAGGCAGC 
CACCAGAGGA ATGCCAATGA CCTTCTCGAC ATTGGATATG TGGGCTCAAA TAGTCAAGGA 
CAGTTTACAG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo2 ( XM_006523049.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523112.1 Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X20, mRNA.

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CloneID	OMu52769
Clone ID Related Accession (Same CDS sequence)	XM_006523049.3 , XM_006523049.4
Accession Version	XM_006523049.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4203bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	roundabout homolog 2 isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006523049.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGCTCCTCC ACAGTTTGTG 
GTTAGGCCAA GAGATCAGAT CGTTGCTCAA GGCCGAACAG TGACATTCCC CTGTGAAACT 
AAAGGAAACC CACAGCCAGC TGTTTTTTGG CAGAAAGAAG GCAGCCAGAA CCTACTTTTC 
CCGAATCAAC CTCAGCAGCC CAACAGCCGA TGTTCAGTGT CGCCCACGGG GGACCTCACC 
ATCACCAACA TCCAGCGTTC AGATGCGGGT TACTACATCT GCCAGGCCCT AACCGTGGCA 
GGAAGCATTT TAGCTAAAGC ACAGTTGGAA GTTACTGACG TTTTGACAGA TAGACCTCCA 
CCCATAATCT TGCAAGGACC AATAAACCAA ACACTTGCAG TAGACGGTAC AGCATTGTTG 
AAGTGTAAAG CCACTGGTGA GCCTCTGCCT GTAATTAGCT GGCTAAAGGA GGGCTTTACT 
TTTCTGGGGA GAGATCCAAG AGCCACGATC CAAGACCAAG GAACACTGCA GATTAAGAAT 
TTACGGATAT CTGATACTGG CACTTATACT TGTGTGGCTA CAAGTTCCAG TGGAGAGACT 
TCCTGGAGTG CAGTGCTGGA TGTAACAGAA TCTGGAGCAA CAATCAGTAA AAATTATGAT 
ATGAATGACC TCCCGGGACC ACCATCCAAA CCTCAGGTCA CTGATGTTTC TAAGAACAGT 
GTCACCTTAT CCTGGCAGCC AGGTACACCT GGCGTTCTTC CTGCAAGCGC GTATATCATT 
GAGGCTTTCA GCCAATCGGT GAGCAATAGC TGGCAGACAG TGGCAAACCA TGTTAAGACA 
ACTCTGTATA CAGTAAGGGG GCTGAGGCCC AACACAATCT ACTTGTTTAT GGTCAGAGCG 
ATCAACCCAC AAGGTCTCAG TGATCCAAGT CCTATGTCGG ATCCTGTACG CACACAAGAT 
ATCAGCCCCC CAGCACAAGG AGTGGACCAC AGACAGGTGC AGAAGGAATT AGGTGATGTC 
GTTGTTCGTC TCCATAATCC AGTTGTCCTG ACACCTACAA CTGTTCAAGT CACATGGACG 
GTGGACCGAC AACCCCAGTT TATTCAGGGC TACAGAGTGA TGTACCGTCA GACTTCGGGA 
CTACAAGCCT CAACTGTGTG GCAGAATCTA GACGCCAAAG TCCCGACTGA GAGGAGTGCT 
GTCCTTGTGA ATTTGAAAAA GGGGGTGACT TATGAAATTA AAGTCCGGCC GTATTTTAAC 
GAGTTCCAAG GAATGGACAG TGAATCGAAA ACAGTCCGAA CCACTGAGGA AGCCCCAAGT 
GCCCCTCCCC AGTCTGTCAC TGTGCTGACA GTTGGAAGTC ACAACAGCAC AAGCATCAGT 
GTTTCCTGGG ATCCTCCACC AGCCGACCAC CAGAATGGAA TTATTCAGGA ATATAAGATC 
TGGTGTCTGG GAAACGAAAC GCGATTCCAT ATCAATAAAA CGGTGGATGC AGCCATTCGC 
TCTGTAGTAA TAGGTGGCTT GTTCCCTGGA ATTCAGTACC GGGTAGAAGT GGCAGCTAGC 
ACAAGTGCAG GGGTTGGAGT AAAAAGTGAA CCACAGCCGA TAATAATTGG GGGACGTAAC 
GAAGTTGTCA TTACTGAAAA CAATAACAGC ATCACTGAGC AAATCACGGA TGTCGTGAAG 
CAACCGGCAT TTATAGCTGG CATTGGTGGT GCCTGCTGGG TAATTCTGAT GGGTTTTAGC 
ATCTGGTTGT ACTGGAGAAG AAAGAAGAGA AAGGGACTCA GCAATTATGC TGTAACATTT 
CAAAGAGGAG ATGGAGGACT AATGAGCAAT GGGAGCCGTC CAGGTCTTCT AAATGCTGGC 
GATCCCAATT ACCCATGGCT TGCTGATTCT TGGCCAGCCA CGAGTTTGCC AGTGAACAAT 
AGCAATAGTG GCCCAAATGA AATTGGAAAT TTTGGGCGTG GAGATGTGCT GCCTCCGGTG 
CCAGGCCAAG GGGATAAAAC AGCGACCATG CTCTCGGATG GAGCCATTTA TAGCAGCATT 
GACTTCACTA CCAAAACCAC TTACAACAGT TCCAGCCAAA TAACACAGGC CACCCCATAT 
GCCACTACAC AAATCCTGCA TTCAAACAGC ATCCACGAAC TGGCAGTTGA TCTTCCTGAT 
CCACAGTGGA AAAGCTCAGT TCAACAGAAG ACAGACCTCA TGGGATTTGG TTATTCGCTA 
CCTGATCAGA ACAAGGGGAA CAACGGTGGG AAAGGTGGAA AAAAGAAGAA AACTAAAAAT 
TCTTCGAAAG CGCAGAAAAA CAACGGATCC ACTTGGGCTA ATGTCCCTCT ACCTCCTCCT 
CCCGTCCAGC CCCTTCCTGG TACAGAGCTG GGCCACTATG CTGCGGAACA AGAAAATGGC 
TATGACAGTG ACAGCTGGTG CCCACCATTA CCGGTGCAAA CATACTTGCA TCAGGGTATG 
GAAGATGAGC TGGAAGAAGA CGAAGATCGG GTCCCAACAC CTCCTGTTCG CGGCGTGGCC 
TCTTCACCAG CTATCTCTTT TGGACAGCAG TCCACTGCCA CTCTTACTCC ATCCCCACGG 
GAAGAGATGC AACCCATGCT GCAAGCTCAC TTGGATGAGT TGACAAGGGC CTATCAGTTT 
GATATAGCAA AACAAACATG GCACATTCAA AGCAATACCC CACCTCCACA ACCCCCAGCT 
CCGCCATTAG GTTACGTGTC CGGAGCCTTG ATTTCTGATT TGGAGACAGA TGTTCCAGAT 
GAGGATGCTG ATGATGAAGA GGAACCATTA GAAATTCCCA GGCCCCTCAG AGCACTAGAC 
CAGACACCTG GATCCAGTAT GGACAATCTA GACAGCTCTG TCACAGGAAA AGCCTTTAGC 
TCCTCTCAAA GGCAGCGGCC CACCAGCCCA TTTTCTACGG ACAGTAACAC CAGTGCTGCC 
CAGAATCAAA GCCAGAGGCC TCGGCCCACG AAAAAACATA AGGGAGGACG GATGGACCCA 
CAGCCAGTGT TACCTCATCG GAGGGAAGGG ATGCCAGATG AGGAGTCCCT GGTGCCCTAC 
AGCAAGCCCA GCTTCCCGTC TCCTGGCGGG CACAGCTCAT CAGGAACATC TTCCTCTAAA 
GGGTCCACTG GCCCTCGGAA GGCTGACGTG CTGAGAGGCA GCCACCAGAG GAATGCCAAT 
GACCTTCTCG ACATTGGATA TGTGGGCTCA AATAGTCAAG GACAGTTTAC AGGTGAATTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523112.1
CDS	337..4539
Translation	MLSSEKVDKITRLELLFVLMLFFSGPTLGKLTRTPWKGKPWKGGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKAMSTDEGTYVCIAENRVGKVEASATLTVRAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPINQTLAVDGTALLKCKATGEPLPVISWLKEGFTFLGRDPRATIQDQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDMNDLPGPPSKPQVTDVSKNSVTLSWQPGTPGVLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVVVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQASTVWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSHNSTSISVSWDPPPADHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVVIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGGRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPNYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTTYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSVQQKTDLMGFGYSLPDQNKGNNGGKGGKKKKTKNSSKAQKNNGSTWANVPLPPPPVQPLPGTELGHYAAEQENGYDSDSWCPPLPVQTYLHQGMEDELEEDEDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNTPPPQPPAPPLGYVSGALISDLETDVPDEDADDEEEPLEIPRPLRALDQTPGSSMDNLDSSVTGKAFSSSQRQRPTSPFSTDSNTSAAQNQSQRPRPTKKHKGGRMDPQPVLPHRREGMPDEESLVPYSKPSFPSPGGHSSSGTSSSKGSTGPRKADVLRGSHQRNANDLLDIGYVGSNSQGQFTGEL

Target ORF information:

RefSeq Version	XM_006523049.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523049.3

ORF Insert Sequence:

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGCTCCTCC ACAGTTTGTG 
GTTAGGCCAA GAGATCAGAT CGTTGCTCAA GGCCGAACAG TGACATTCCC CTGTGAAACT 
AAAGGAAACC CACAGCCAGC TGTTTTTTGG CAGAAAGAAG GCAGCCAGAA CCTACTTTTC 
CCGAATCAAC CTCAGCAGCC CAACAGCCGA TGTTCAGTGT CGCCCACGGG GGACCTCACC 
ATCACCAACA TCCAGCGTTC AGATGCGGGT TACTACATCT GCCAGGCCCT AACCGTGGCA 
GGAAGCATTT TAGCTAAAGC ACAGTTGGAA GTTACTGACG TTTTGACAGA TAGACCTCCA 
CCCATAATCT TGCAAGGACC AATAAACCAA ACACTTGCAG TAGACGGTAC AGCATTGTTG 
AAGTGTAAAG CCACTGGTGA GCCTCTGCCT GTAATTAGCT GGCTAAAGGA GGGCTTTACT 
TTTCTGGGGA GAGATCCAAG AGCCACGATC CAAGACCAAG GAACACTGCA GATTAAGAAT 
TTACGGATAT CTGATACTGG CACTTATACT TGTGTGGCTA CAAGTTCCAG TGGAGAGACT 
TCCTGGAGTG CAGTGCTGGA TGTAACAGAA TCTGGAGCAA CAATCAGTAA AAATTATGAT 
ATGAATGACC TCCCGGGACC ACCATCCAAA CCTCAGGTCA CTGATGTTTC TAAGAACAGT 
GTCACCTTAT CCTGGCAGCC AGGTACACCT GGCGTTCTTC CTGCAAGCGC GTATATCATT 
GAGGCTTTCA GCCAATCGGT GAGCAATAGC TGGCAGACAG TGGCAAACCA TGTTAAGACA 
ACTCTGTATA CAGTAAGGGG GCTGAGGCCC AACACAATCT ACTTGTTTAT GGTCAGAGCG 
ATCAACCCAC AAGGTCTCAG TGATCCAAGT CCTATGTCGG ATCCTGTACG CACACAAGAT 
ATCAGCCCCC CAGCACAAGG AGTGGACCAC AGACAGGTGC AGAAGGAATT AGGTGATGTC 
GTTGTTCGTC TCCATAATCC AGTTGTCCTG ACACCTACAA CTGTTCAAGT CACATGGACG 
GTGGACCGAC AACCCCAGTT TATTCAGGGC TACAGAGTGA TGTACCGTCA GACTTCGGGA 
CTACAAGCCT CAACTGTGTG GCAGAATCTA GACGCCAAAG TCCCGACTGA GAGGAGTGCT 
GTCCTTGTGA ATTTGAAAAA GGGGGTGACT TATGAAATTA AAGTCCGGCC GTATTTTAAC 
GAGTTCCAAG GAATGGACAG TGAATCGAAA ACAGTCCGAA CCACTGAGGA AGCCCCAAGT 
GCCCCTCCCC AGTCTGTCAC TGTGCTGACA GTTGGAAGTC ACAACAGCAC AAGCATCAGT 
GTTTCCTGGG ATCCTCCACC AGCCGACCAC CAGAATGGAA TTATTCAGGA ATATAAGATC 
TGGTGTCTGG GAAACGAAAC GCGATTCCAT ATCAATAAAA CGGTGGATGC AGCCATTCGC 
TCTGTAGTAA TAGGTGGCTT GTTCCCTGGA ATTCAGTACC GGGTAGAAGT GGCAGCTAGC 
ACAAGTGCAG GGGTTGGAGT AAAAAGTGAA CCACAGCCGA TAATAATTGG GGGACGTAAC 
GAAGTTGTCA TTACTGAAAA CAATAACAGC ATCACTGAGC AAATCACGGA TGTCGTGAAG 
CAACCGGCAT TTATAGCTGG CATTGGTGGT GCCTGCTGGG TAATTCTGAT GGGTTTTAGC 
ATCTGGTTGT ACTGGAGAAG AAAGAAGAGA AAGGGACTCA GCAATTATGC TGTAACATTT 
CAAAGAGGAG ATGGAGGACT AATGAGCAAT GGGAGCCGTC CAGGTCTTCT AAATGCTGGC 
GATCCCAATT ACCCATGGCT TGCTGATTCT TGGCCAGCCA CGAGTTTGCC AGTGAACAAT 
AGCAATAGTG GCCCAAATGA AATTGGAAAT TTTGGGCGTG GAGATGTGCT GCCTCCGGTG 
CCAGGCCAAG GGGATAAAAC AGCGACCATG CTCTCGGATG GAGCCATTTA TAGCAGCATT 
GACTTCACTA CCAAAACCAC TTACAACAGT TCCAGCCAAA TAACACAGGC CACCCCATAT 
GCCACTACAC AAATCCTGCA TTCAAACAGC ATCCACGAAC TGGCAGTTGA TCTTCCTGAT 
CCACAGTGGA AAAGCTCAGT TCAACAGAAG ACAGACCTCA TGGGATTTGG TTATTCGCTA 
CCTGATCAGA ACAAGGGGAA CAACGGTGGG AAAGGTGGAA AAAAGAAGAA AACTAAAAAT 
TCTTCGAAAG CGCAGAAAAA CAACGGATCC ACTTGGGCTA ATGTCCCTCT ACCTCCTCCT 
CCCGTCCAGC CCCTTCCTGG TACAGAGCTG GGCCACTATG CTGCGGAACA AGAAAATGGC 
TATGACAGTG ACAGCTGGTG CCCACCATTA CCGGTGCAAA CATACTTGCA TCAGGGTATG 
GAAGATGAGC TGGAAGAAGA CGAAGATCGG GTCCCAACAC CTCCTGTTCG CGGCGTGGCC 
TCTTCACCAG CTATCTCTTT TGGACAGCAG TCCACTGCCA CTCTTACTCC ATCCCCACGG 
GAAGAGATGC AACCCATGCT GCAAGCTCAC TTGGATGAGT TGACAAGGGC CTATCAGTTT 
GATATAGCAA AACAAACATG GCACATTCAA AGCAATACCC CACCTCCACA ACCCCCAGCT 
CCGCCATTAG GTTACGTGTC CGGAGCCTTG ATTTCTGATT TGGAGACAGA TGTTCCAGAT 
GAGGATGCTG ATGATGAAGA GGAACCATTA GAAATTCCCA GGCCCCTCAG AGCACTAGAC 
CAGACACCTG GATCCAGTAT GGACAATCTA GACAGCTCTG TCACAGGAAA AGCCTTTAGC 
TCCTCTCAAA GGCAGCGGCC CACCAGCCCA TTTTCTACGG ACAGTAACAC CAGTGCTGCC 
CAGAATCAAA GCCAGAGGCC TCGGCCCACG AAAAAACATA AGGGAGGACG GATGGACCCA 
CAGCCAGTGT TACCTCATCG GAGGGAAGGG ATGCCAGATG AGGAGTCCCT GGTGCCCTAC 
AGCAAGCCCA GCTTCCCGTC TCCTGGCGGG CACAGCTCAT CAGGAACATC TTCCTCTAAA 
GGGTCCACTG GCCCTCGGAA GGCTGACGTG CTGAGAGGCA GCCACCAGAG GAATGCCAAT 
GACCTTCTCG ACATTGGATA TGTGGGCTCA AATAGTCAAG GACAGTTTAC AGGTGAATTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo2 ( XM_011246131.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244433.1 Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OMu74355
Clone ID Related Accession (Same CDS sequence)	XM_011246131.2 , XM_011246131.3
Accession Version	XM_011246131.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4398bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	roundabout homolog 2 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011246131.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT 
CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG 
GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC 
CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA 
GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT 
ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG 
GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG 
CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT 
AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT 
TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC 
GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC 
CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT 
ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA 
CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA 
TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA 
GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT 
CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT 
GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG 
CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG 
GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC 
ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG 
GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT 
GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA 
GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT 
GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG 
GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT 
GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT 
CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG 
CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG 
CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT 
TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG 
GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT 
GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT 
GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGGTG GGAAAGGTGG AAAAAAGAAG 
AAAACTAAAA ATTCTTCGAA AGCGCAGAAA AACAACGGAT CCACTTGGGC TAATGTCCCT 
CTACCTCCTC CTCCCGTCCA GCCCCTTCCT GGTACAGAGC TGGGCCACTA TGCTGCGGAA 
CAAGAAAATG GCTATGACAG TGACAGCTGG TGCCCACCAT TACCGGTGCA AACATACTTG 
CATCAGGGTA TGGAAGATGA GCTGGAAGAA GACGAAGATC GGGTCCCAAC ACCTCCTGTT 
CGCGGCGTGG CCTCTTCACC AGCTATCTCT TTTGGACAGC AGTCCACTGC CACTCTTACT 
CCATCCCCAC GGGAAGAGAT GCAACCCATG CTGCAAGCTC ACTTGGATGA GTTGACAAGG 
GCCTATCAGT TTGATATAGC AAAACAAACA TGGCACATTC AAAGCAATAC CCCACCTCCA 
CAACCCCCAG CTCCGCCATT AGGTTACGTG TCCGGAGCCT TGATTTCTGA TTTGGAGACA 
GATGTTCCAG ATGAGGATGC TGATGATGAA GAGGAACCAT TAGAAATTCC CAGGCCCCTC 
AGAGCACTAG ACCAGACACC TGGATCCAGT ATGGACAATC TAGACAGCTC TGTCACAGGT 
TCAATGGTAA ATGGATGGGG CTCTGCATCT GATGAGGATC GTAACTTTTC TAGTCATAGA 
TCTAGTGTAG GTAGCTCCTC AGATGGCTCC ATCTTTGCCA GCGGCAGCTT TGCAAAAGCA 
TTGGTGGCAG CAGCAGACAA AGCTGGCTTT AGGCTGGATG GAACCAGTCT TACGAGAACA 
GGAAAAGCCT TTAGCTCCTC TCAAAGGCAG CGGCCCACCA GCCCATTTTC TACGGACAGT 
AACACCAGTG CTGCCCAGAA TCAAAGCCAG AGGCCTCGGC CCACGAAAAA ACATAAGGGA 
GGACGGATGG ACCCACAGCC AGTGTTACCT CATCGGAGGG AAGGGATGCC AGATGAGGAG 
TCCCTGGTGC CCTACAGCAA GCCCAGCTTC CCGTCTCCTG GCGGGCACAG CTCATCAGGA 
ACATCTTCCT CTAAAGGGTC CACTGGCCCT CGGAAGGCTG ACGTGCTGAG AGGCAGCCAC 
CAGAGGAATG CCAATGACCT TCTCGACATT GGATATGTGG GCTCAAATAG TCAAGGACAG 
TTTACAGGTG AATTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244433.1
CDS	337..4734
Translation	MLSSEKVDKITRLELLFVLMLFFSGPTLGKLTRTPWKGKPWKGGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKAMSTDEGTYVCIAENRVGKVEASATLTVRVRPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPINQTLAVDGTALLKCKATGEPLPVISWLKEGFTFLGRDPRATIQDQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDMNDLPGPPSKPQVTDVSKNSVTLSWQPGTPGVLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVVVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQASTVWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSHNSTSISVSWDPPPADHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVVIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGGRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPNYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTTYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSVQQKTDLMGFGYSLPDQNKGNNGGKGGKKKKTKNSSKAQKNNGSTWANVPLPPPPVQPLPGTELGHYAAEQENGYDSDSWCPPLPVQTYLHQGMEDELEEDEDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNTPPPQPPAPPLGYVSGALISDLETDVPDEDADDEEEPLEIPRPLRALDQTPGSSMDNLDSSVTGSMVNGWGSASDEDRNFSSHRSSVGSSSDGSIFASGSFAKALVAAADKAGFRLDGTSLTRTGKAFSSSQRQRPTSPFSTDSNTSAAQNQSQRPRPTKKHKGGRMDPQPVLPHRREGMPDEESLVPYSKPSFPSPGGHSSSGTSSSKGSTGPRKADVLRGSHQRNANDLLDIGYVGSNSQGQFTGEL

Target ORF information:

RefSeq Version	XM_011246131.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011246131.2

ORF Insert Sequence:

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT 
CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG 
GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC 
CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA 
GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT 
ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG 
GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG 
CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT 
AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT 
TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC 
GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC 
CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT 
ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA 
CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA 
TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA 
GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT 
CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT 
GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG 
CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG 
GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC 
ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG 
GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT 
GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA 
GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT 
GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG 
GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT 
GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT 
CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG 
CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG 
CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT 
TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG 
GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT 
GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT 
GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGGTG GGAAAGGTGG AAAAAAGAAG 
AAAACTAAAA ATTCTTCGAA AGCGCAGAAA AACAACGGAT CCACTTGGGC TAATGTCCCT 
CTACCTCCTC CTCCCGTCCA GCCCCTTCCT GGTACAGAGC TGGGCCACTA TGCTGCGGAA 
CAAGAAAATG GCTATGACAG TGACAGCTGG TGCCCACCAT TACCGGTGCA AACATACTTG 
CATCAGGGTA TGGAAGATGA GCTGGAAGAA GACGAAGATC GGGTCCCAAC ACCTCCTGTT 
CGCGGCGTGG CCTCTTCACC AGCTATCTCT TTTGGACAGC AGTCCACTGC CACTCTTACT 
CCATCCCCAC GGGAAGAGAT GCAACCCATG CTGCAAGCTC ACTTGGATGA GTTGACAAGG 
GCCTATCAGT TTGATATAGC AAAACAAACA TGGCACATTC AAAGCAATAC CCCACCTCCA 
CAACCCCCAG CTCCGCCATT AGGTTACGTG TCCGGAGCCT TGATTTCTGA TTTGGAGACA 
GATGTTCCAG ATGAGGATGC TGATGATGAA GAGGAACCAT TAGAAATTCC CAGGCCCCTC 
AGAGCACTAG ACCAGACACC TGGATCCAGT ATGGACAATC TAGACAGCTC TGTCACAGGT 
TCAATGGTAA ATGGATGGGG CTCTGCATCT GATGAGGATC GTAACTTTTC TAGTCATAGA 
TCTAGTGTAG GTAGCTCCTC AGATGGCTCC ATCTTTGCCA GCGGCAGCTT TGCAAAAGCA 
TTGGTGGCAG CAGCAGACAA AGCTGGCTTT AGGCTGGATG GAACCAGTCT TACGAGAACA 
GGAAAAGCCT TTAGCTCCTC TCAAAGGCAG CGGCCCACCA GCCCATTTTC TACGGACAGT 
AACACCAGTG CTGCCCAGAA TCAAAGCCAG AGGCCTCGGC CCACGAAAAA ACATAAGGGA 
GGACGGATGG ACCCACAGCC AGTGTTACCT CATCGGAGGG AAGGGATGCC AGATGAGGAG 
TCCCTGGTGC CCTACAGCAA GCCCAGCTTC CCGTCTCCTG GCGGGCACAG CTCATCAGGA 
ACATCTTCCT CTAAAGGGTC CACTGGCCCT CGGAAGGCTG ACGTGCTGAG AGGCAGCCAC 
CAGAGGAATG CCAATGACCT TCTCGACATT GGATATGTGG GCTCAAATAG TCAAGGACAG 
TTTACAGGTG AATTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Robo2 ( XM_011246132.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244434.1 Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X18, mRNA.

Price & Availability↑

CloneID	OMu74356
Clone ID Related Accession (Same CDS sequence)	NM_001358492.1 , XM_011246132.2
Accession Version	XM_011246132.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4215bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	roundabout homolog 2 isoform X18
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011246132.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT 
CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG 
GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC 
CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA 
GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT 
ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG 
GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG 
CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT 
AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT 
TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC 
GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC 
CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT 
ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA 
CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA 
TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA 
GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT 
CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT 
GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG 
CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG 
GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC 
ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG 
GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT 
GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA 
GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT 
GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG 
GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT 
GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT 
CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG 
CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG 
CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT 
TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG 
GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT 
GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT 
GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGGTG GGAAAGGTGG AAAAAAGAAG 
AAAACTAAAA ATTCTTCGAA AGCGCAGAAA AACAACGGAT CCACTTGGGC TAATGTCCCT 
CTACCTCCTC CTCCCGTCCA GCCCCTTCCT GGTACAGAGC TGGGCCACTA TGCTGCGGAA 
CAAGAAAATG GCTATGACAG TGACAGCTGG TGCCCACCAT TACCGGTGCA AACATACTTG 
CATCAGGGTA TGGAAGATGA GCTGGAAGAA GACGAAGATC GGGTCCCAAC ACCTCCTGTT 
CGCGGCGTGG CCTCTTCACC AGCTATCTCT TTTGGACAGC AGTCCACTGC CACTCTTACT 
CCATCCCCAC GGGAAGAGAT GCAACCCATG CTGCAAGCTC ACTTGGATGA GTTGACAAGG 
GCCTATCAGT TTGATATAGC AAAACAAACA TGGCACATTC AAAGCAATAC CCCACCTCCA 
CAACCCCCAG CTCCGCCATT AGGTTACGTG TCCGGAGCCT TGATTTCTGA TTTGGAGACA 
GATGTTCCAG ATGAGGATGC TGATGATGAA GAGGAACCAT TAGAAATTCC CAGGCCCCTC 
AGAGCACTAG ACCAGACACC TGGATCCAGT ATGGACAATC TAGACAGCTC TGTCACAGGA 
AAAGCCTTTA GCTCCTCTCA AAGGCAGCGG CCCACCAGCC CATTTTCTAC GGACAGTAAC 
ACCAGTGCTG CCCAGAATCA AAGCCAGAGG CCTCGGCCCA CGAAAAAACA TAAGGGAGGA 
CGGATGGACC CACAGCCAGT GTTACCTCAT CGGAGGGAAG GGATGCCAGA TGAGGAGTCC 
CTGGTGCCCT ACAGCAAGCC CAGCTTCCCG TCTCCTGGCG GGCACAGCTC ATCAGGAACA 
TCTTCCTCTA AAGGGTCCAC TGGCCCTCGG AAGGCTGACG TGCTGAGAGG CAGCCACCAG 
AGGAATGCCA ATGACCTTCT CGACATTGGA TATGTGGGCT CAAATAGTCA AGGACAGTTT 
ACAGGTGAAT TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244434.1
CDS	337..4551
Translation	MLSSEKVDKITRLELLFVLMLFFSGPTLGKLTRTPWKGKPWKGGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKAMSTDEGTYVCIAENRVGKVEASATLTVRVRPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPINQTLAVDGTALLKCKATGEPLPVISWLKEGFTFLGRDPRATIQDQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDMNDLPGPPSKPQVTDVSKNSVTLSWQPGTPGVLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVVVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQASTVWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSHNSTSISVSWDPPPADHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVVIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGGRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPNYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTTYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSVQQKTDLMGFGYSLPDQNKGNNGGKGGKKKKTKNSSKAQKNNGSTWANVPLPPPPVQPLPGTELGHYAAEQENGYDSDSWCPPLPVQTYLHQGMEDELEEDEDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNTPPPQPPAPPLGYVSGALISDLETDVPDEDADDEEEPLEIPRPLRALDQTPGSSMDNLDSSVTGKAFSSSQRQRPTSPFSTDSNTSAAQNQSQRPRPTKKHKGGRMDPQPVLPHRREGMPDEESLVPYSKPSFPSPGGHSSSGTSSSKGSTGPRKADVLRGSHQRNANDLLDIGYVGSNSQGQFTGEL

Target ORF information:

RefSeq Version	XM_011246132.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus roundabout guidance receptor 2 (Robo2), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011246132.2

ORF Insert Sequence:

ATGTTATCAT CCGAGAAAGT GGACAAAATT ACTAGACTCG AACTTCTCTT CGTTTTGATG 
TTATTCTTCT CAGGACCAAC TCTAGGCAAG CTGACCAGGA CCCCCTGGAA GGGCAAACCT 
TGGAAGGGGG GATCTCGTCT TCGTCAAGAA GACTTTCCCC CCAGGATCGT AGAACACCCT 
TCTGATGTCA TCGTCTCCAA GGGAGAGCCC ACCACTCTGA ACTGTAAAGC AGAGGGCCGA 
CCCACCCCCA CCATTGAATG GTACAAGGAT GGTGAGAGGG TGGAGACAGA CAAGGATGAT 
CCCAGGTCCC ACAGAATGCT TCTGCCCAGC GGATCTTTAT TCTTTTTGCG AATTGTTCAT 
GGGCGCAGAA GTAAACCGGA CGAAGGGAGT TACGTTTGTG TTGCAAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC ATCTCTGGAA GTGGCATTAT TGCGAGATGA CTTCCGGCAA 
AACCCCACAG ATGTGGTAGT CGCAGCTGGA GAGCCTGCAA TCTTGGAGTG CCAGCCACCA 
CGGGGACACC CAGAACCAAC CATCTACTGG AAAAAGGACA AAGTCCGAAT TGATGACAAG 
GAAGAGAGAA TAAGTATCCG TGGTGGGAAG CTGATGATCT CTAATACTAG GAAAAGCGAT 
GCTGGCATGT ACACCTGTGT GGGAACCAAT ATGGTGGGAG AAAGGGACAG CGACCCTGCA 
GAGCTCACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTGCTA 
GAGGAAGAAG CTGTAGAATT CCGTTGTCAG GTCCAAGGAG ATCCCCAGCC AACGGTGAGG 
TGGAAAAAAG ATGATGCAGA CTTGCCGAGA GGAAGGTATG ATATCAAAGA TGACTACACG 
CTGAGAATTA AAAAGGCCAT GAGTACTGAT GAAGGTACCT ATGTGTGTAT TGCTGAGAAT 
CGGGTGGGAA AAGTGGAAGC CTCTGCTACC CTCACTGTCC GAGTTCGCCC TGTTGCTCCT 
CCACAGTTTG TGGTTAGGCC AAGAGATCAG ATCGTTGCTC AAGGCCGAAC AGTGACATTC 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCGAATCA ACCTCAGCAG CCCAACAGCC GATGTTCAGT GTCGCCCACG 
GGGGACCTCA CCATCACCAA CATCCAGCGT TCAGATGCGG GTTACTACAT CTGCCAGGCC 
CTAACCGTGG CAGGAAGCAT TTTAGCTAAA GCACAGTTGG AAGTTACTGA CGTTTTGACA 
GATAGACCTC CACCCATAAT CTTGCAAGGA CCAATAAACC AAACACTTGC AGTAGACGGT 
ACAGCATTGT TGAAGTGTAA AGCCACTGGT GAGCCTCTGC CTGTAATTAG CTGGCTAAAG 
GAGGGCTTTA CTTTTCTGGG GAGAGATCCA AGAGCCACGA TCCAAGACCA AGGAACACTG 
CAGATTAAGA ATTTACGGAT ATCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCC 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTAACAG AATCTGGAGC AACAATCAGT 
AAAAATTATG ATATGAATGA CCTCCCGGGA CCACCATCCA AACCTCAGGT CACTGATGTT 
TCTAAGAACA GTGTCACCTT ATCCTGGCAG CCAGGTACAC CTGGCGTTCT TCCTGCAAGC 
GCGTATATCA TTGAGGCTTT CAGCCAATCG GTGAGCAATA GCTGGCAGAC AGTGGCAAAC 
CATGTTAAGA CAACTCTGTA TACAGTAAGG GGGCTGAGGC CCAACACAAT CTACTTGTTT 
ATGGTCAGAG CGATCAACCC ACAAGGTCTC AGTGATCCAA GTCCTATGTC GGATCCTGTA 
CGCACACAAG ATATCAGCCC CCCAGCACAA GGAGTGGACC ACAGACAGGT GCAGAAGGAA 
TTAGGTGATG TCGTTGTTCG TCTCCATAAT CCAGTTGTCC TGACACCTAC AACTGTTCAA 
GTCACATGGA CGGTGGACCG ACAACCCCAG TTTATTCAGG GCTACAGAGT GATGTACCGT 
CAGACTTCGG GACTACAAGC CTCAACTGTG TGGCAGAATC TAGACGCCAA AGTCCCGACT 
GAGAGGAGTG CTGTCCTTGT GAATTTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTCCGG 
CCGTATTTTA ACGAGTTCCA AGGAATGGAC AGTGAATCGA AAACAGTCCG AACCACTGAG 
GAAGCCCCAA GTGCCCCTCC CCAGTCTGTC ACTGTGCTGA CAGTTGGAAG TCACAACAGC 
ACAAGCATCA GTGTTTCCTG GGATCCTCCA CCAGCCGACC ACCAGAATGG AATTATTCAG 
GAATATAAGA TCTGGTGTCT GGGAAACGAA ACGCGATTCC ATATCAATAA AACGGTGGAT 
GCAGCCATTC GCTCTGTAGT AATAGGTGGC TTGTTCCCTG GAATTCAGTA CCGGGTAGAA 
GTGGCAGCTA GCACAAGTGC AGGGGTTGGA GTAAAAAGTG AACCACAGCC GATAATAATT 
GGGGGACGTA ACGAAGTTGT CATTACTGAA AACAATAACA GCATCACTGA GCAAATCACG 
GATGTCGTGA AGCAACCGGC ATTTATAGCT GGCATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATCTGGTT GTACTGGAGA AGAAAGAAGA GAAAGGGACT CAGCAATTAT 
GCTGTAACAT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGGAGCCG TCCAGGTCTT 
CTAAATGCTG GCGATCCCAA TTACCCATGG CTTGCTGATT CTTGGCCAGC CACGAGTTTG 
CCAGTGAACA ATAGCAATAG TGGCCCAAAT GAAATTGGAA ATTTTGGGCG TGGAGATGTG 
CTGCCTCCGG TGCCAGGCCA AGGGGATAAA ACAGCGACCA TGCTCTCGGA TGGAGCCATT 
TATAGCAGCA TTGACTTCAC TACCAAAACC ACTTACAACA GTTCCAGCCA AATAACACAG 
GCCACCCCAT ATGCCACTAC ACAAATCCTG CATTCAAACA GCATCCACGA ACTGGCAGTT 
GATCTTCCTG ATCCACAGTG GAAAAGCTCA GTTCAACAGA AGACAGACCT CATGGGATTT 
GGTTATTCGC TACCTGATCA GAACAAGGGG AACAACGGTG GGAAAGGTGG AAAAAAGAAG 
AAAACTAAAA ATTCTTCGAA AGCGCAGAAA AACAACGGAT CCACTTGGGC TAATGTCCCT 
CTACCTCCTC CTCCCGTCCA GCCCCTTCCT GGTACAGAGC TGGGCCACTA TGCTGCGGAA 
CAAGAAAATG GCTATGACAG TGACAGCTGG TGCCCACCAT TACCGGTGCA AACATACTTG 
CATCAGGGTA TGGAAGATGA GCTGGAAGAA GACGAAGATC GGGTCCCAAC ACCTCCTGTT 
CGCGGCGTGG CCTCTTCACC AGCTATCTCT TTTGGACAGC AGTCCACTGC CACTCTTACT 
CCATCCCCAC GGGAAGAGAT GCAACCCATG CTGCAAGCTC ACTTGGATGA GTTGACAAGG 
GCCTATCAGT TTGATATAGC AAAACAAACA TGGCACATTC AAAGCAATAC CCCACCTCCA 
CAACCCCCAG CTCCGCCATT AGGTTACGTG TCCGGAGCCT TGATTTCTGA TTTGGAGACA 
GATGTTCCAG ATGAGGATGC TGATGATGAA GAGGAACCAT TAGAAATTCC CAGGCCCCTC 
AGAGCACTAG ACCAGACACC TGGATCCAGT ATGGACAATC TAGACAGCTC TGTCACAGGA 
AAAGCCTTTA GCTCCTCTCA AAGGCAGCGG CCCACCAGCC CATTTTCTAC GGACAGTAAC 
ACCAGTGCTG CCCAGAATCA AAGCCAGAGG CCTCGGCCCA CGAAAAAACA TAAGGGAGGA 
CGGATGGACC CACAGCCAGT GTTACCTCAT CGGAGGGAAG GGATGCCAGA TGAGGAGTCC 
CTGGTGCCCT ACAGCAAGCC CAGCTTCCCG TCTCCTGGCG GGCACAGCTC ATCAGGAACA 
TCTTCCTCTA AAGGGTCCAC TGGCCCTCGG AAGGCTGACG TGCTGAGAGG CAGCCACCAG 
AGGAATGCCA ATGACCTTCT CGACATTGGA TATGTGGGCT CAAATAGTCA AGGACAGTTT 
ACAGGTGAAT TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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Robo2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information: