Cln8 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cln8
Entrez Gene ID	26889
Full Name	ceroid-lipofuscinosis, neuronal 8
Synonyms	mnd
General protein information	Preferred Names ceroid-lipofuscinosis, neuronal 8 Names protein CLN8 motor neuron degeneration
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8 A1.1\|8 7.59 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006508806.3	XP_006508869.1	protein CLN8 isoform X1
XM_006508806.4	XP_006508869.1	protein CLN8 isoform X1
NM_012000.3	NP_036130.1	protein CLN8
XM_006508806.2	XP_006508869.1	protein CLN8 isoform X1
XM_006508807.2	XP_006508870.1	protein CLN8 isoform X1

Pathways from BioSystems

Homologies

	CLN8	XP_001096231.1
Bos taurus (cattle)	CLN8	NP_001069409.2
Xenopus tropicalis (tropical clawed frog)	cln8	XP_004914543.1
Homo sapiens (human)	CLN8	NP_061764.2
Canis lupus familiaris (dog)	CLN8	NP_001012343.1
Mus musculus (house mouse)	Cln8	NP_036130.1
Gallus gallus (chicken)	CLN8	NP_001026258.1
Pan troglodytes (chimpanzee)	CLN8	XP_519584.2
Rattus norvegicus (Norway rat)	Cln8	NP_001007687.1
Danio rerio (zebrafish)	cln8	NP_001108060.1

Gene Ontology
Bibliography

Related articles in PubMed

NCL disease mechanisms.
Palmer DN, Barry LA, Tyynel? J, Cooper JD
Biochimica et biophysica acta1832(11)1882-93(2013 Nov)

Identifying protein partners of CLN8, an ER-resident protein involved in neuronal ceroid lipofuscinosis.
Passantino R, Cascio C, Deidda I, Galizzi G, Russo D, Spedale G, Guarneri P
Biochimica et biophysica acta1833(3)529-40(2013 Mar)

Selective spatiotemporal patterns of glial activation and neuron loss in the sensory thalamocortical pathways of neuronal ceroid lipofuscinosis 8 mice.
Kuronen M, Lehesjoki AE, Jalanko A, Cooper JD, Kopra O
Neurobiology of disease47(3)444-57(2012 Sep)

Increased [?H]D-aspartate release and changes in glutamate receptor expression in the hippocampus of the mnd mouse.
Bigini P, Milanese M, Gardoni F, Longhi A, Bonifacino T, Barbera S, Fumagalli E, Di Luca M, Mennini T, Bonanno G
Journal of neuroscience research90(6)1148-58(2012 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study demonistrated that Selective spatiotemporal patterns of glial activation and neuron loss in the sensory thalamocortical pathways in neuronal ceroid lipofuscinosis 8 decifiency mice.
Title: Selective spatiotemporal patterns of glial activation and neuron loss in the sensory thalamocortical pathways of neuronal ceroid lipofuscinosis 8 mice.

The amount of myelin was reduced in 1-month-old Cln8(mnd) mice, but reached normal levels by 5 months of age. The level of Cln8 gene expression followed the developmental pattern of myelin formation and was high in primary oligodendrocytes.
Title: Galactolipid deficiency in the early pathogenesis of neuronal ceroid lipofuscinosis model Cln8mnd : implications to delayed myelination and oligodendrocyte maturation.

This study demonistrated that the changes in glutamate receptor expression in the hippocampus of the mnd mouse.
Title: Increased [?H]D-aspartate release and changes in glutamate receptor expression in the hippocampus of the mnd mouse.

Mutations in mouse CLN8 gene show marked deficiency of mitochondrial Ca2+ clearance in Cln8mnd neurons, and the heightened vulnerability of these neurons to excitotoxic effects of glutamate.
Title: Deficient mitochondrial Ca(2+) buffering in the Cln8(mnd) mouse model of neuronal ceroid lipofuscinosis.

Expression of Cln8 in the developing and mature brain suggests roles for Cln8 in maturation, differentiation and supporting the survival of different neuronal populations.
Title: The neuronal ceroid lipofuscinosis Cln8 gene expression is developmentally regulated in mouse brain and up-regulated in the hippocampal kindling model of epilepsy.

The following Cln8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cln8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu09299	NM_012000.3 Latest version!	Mus musculus CLN8 transmembrane ER and ERGIC protein (Cln8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu09299	XM_006508806.4 Latest version!	Mus musculus ceroid-lipofuscinosis, neuronal 8 (Cln8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu09299	XM_006508807.2 Latest version!	Mus musculus ceroid-lipofuscinosis, neuronal 8 (Cln8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
View Old Accession Versions >>
OMu09299	XM_006508806.3	Mus musculus ceroid-lipofuscinosis, neuronal 8 (Cln8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu09299	XM_006508806.2	Mus musculus ceroid-lipofuscinosis, neuronal 8 (Cln8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cln8 ( NM_012000.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_036130.1 Mus musculus CLN8 transmembrane ER and ERGIC protein (Cln8), mRNA.

Price & Availability↑

CloneID	OMu09299
Clone ID Related Accession (Same CDS sequence)	XM_006508806.3 , XM_006508806.4 , NM_012000.3 , XM_006508806.2 , XM_006508807.2
Accession Version	NM_012000.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 867bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-19
Organism	Mus musculus(house mouse)
Product	protein CLN8
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK166905.1 and AC108844.8. On Sep 21, 2006 this sequence version replaced NM_012000.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK166905.1, AK049145.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACTCCTG TGAGCAGCCA CGGCTTGGCG GAGAGCATTT TTGACCTGGA CTACGCTTCG 
TGGAAGATTC GGTCGACTCT AGCGGTTGCT GGCTTTGTCT TCTACCTGGG CGTCTTTGTA 
GTCTGCCATC AGCTCTCATC GTCCCTGAAT GCCACCTACC GCTCCCTGGC GGCCAAAGAG 
AAGGTCTTCT GGAACCTGGC GGCGACGCGT GCTGTCTTCG GCGTCCAGAG CACAACTGCA 
GGCCTGTGGG CCCTGCTGGG AGACCCCGTG CTCTATGCCG ACAAAGCTCT CGGGCAGCAG 
AACTGGTGTT GGTTTCACAT CACCACGGCC ACTGGCTTCT TCTTCTTTGA GAATGTGGCC 
GTTCACCTGT CCAACCTGTT CTTCCGCACC TTTGACTTGT TTCTGGTTGT CCACCACCTC 
TTTGCCTTTC TTGGGTTCCT GGGTTCAGCG ATCAATCTCA GAGCTGGCCA CTATCTAGCC 
ATGACCACGT TGCTCCTAGA GATGAGCACG CCCTTCACCT GCATTTCCTG GATGCTCCTG 
AAGGCTGGGT GGTCAGACTC CCTGTTCTGG AAGGCCAACC AGTGGCTAAT GATCCACATG 
TTTCACTGTC GAATGATCCT CACCTACCAC ATGTGGTGGG TGTGCTTCTG TCACTGGGAC 
GCCCTCACCA GCAGCCTGCA CCTGCCCCAC TGGGCACTGT TCCTTTTCGG GCTGGCCCTG 
CTCACGGCCG TCATTAACCC TTACTGGACA CACAAGAAGA CCCAGCAGCT CCTCCATCCC 
GTGGATTGGA ACTTTGCACA GGAGGAAGCC AAGGGCAGCA GGCAAGAAAG GACCAATGGC 
CAGGTGCCTC GGAAAAAGAG GTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036130.1
CDS	700..1566
Translation	MTPVSSHGLAESIFDLDYASWKIRSTLAVAGFVFYLGVFVVCHQLSSSLNATYRSLAAKEKVFWNLAATRAVFGVQSTTAGLWALLGDPVLYADKALGQQNWCWFHITTATGFFFFENVAVHLSNLFFRTFDLFLVVHHLFAFLGFLGSAINLRAGHYLAMTTLLLEMSTPFTCISWMLLKAGWSDSLFWKANQWLMIHMFHCRMILTYHMWWVCFCHWDALTSSLHLPHWALFLFGLALLTAVINPYWTHKKTQQLLHPVDWNFAQEEAKGSRQERTNGQVPRKKRL

Target ORF information:

RefSeq Version	NM_012000.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus CLN8 transmembrane ER and ERGIC protein (Cln8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012000.3

ORF Insert Sequence:

ATGACTCCTG TGAGCAGCCA CGGCTTGGCG GAGAGCATTT TTGACCTGGA CTACGCTTCG 
TGGAAGATTC GGTCGACTCT AGCGGTTGCT GGCTTTGTCT TCTACCTGGG CGTCTTTGTA 
GTCTGCCATC AGCTCTCATC GTCCCTGAAT GCCACCTACC GCTCCCTGGC GGCCAAAGAG 
AAGGTCTTCT GGAACCTGGC GGCGACGCGT GCTGTCTTCG GCGTCCAGAG CACAACTGCA 
GGCCTGTGGG CCCTGCTGGG AGACCCCGTG CTCTATGCCG ACAAAGCTCT CGGGCAGCAG 
AACTGGTGTT GGTTTCACAT CACCACGGCC ACTGGCTTCT TCTTCTTTGA GAATGTGGCC 
GTTCACCTGT CCAACCTGTT CTTCCGCACC TTTGACTTGT TTCTGGTTGT CCACCACCTC 
TTTGCCTTTC TTGGGTTCCT GGGTTCAGCG ATCAATCTCA GAGCTGGCCA CTATCTAGCC 
ATGACCACGT TGCTCCTAGA GATGAGCACG CCCTTCACCT GCATTTCCTG GATGCTCCTG 
AAGGCTGGGT GGTCAGACTC CCTGTTCTGG AAGGCCAACC AGTGGCTAAT GATCCACATG 
TTTCACTGTC GAATGATCCT CACCTACCAC ATGTGGTGGG TGTGCTTCTG TCACTGGGAC 
GCCCTCACCA GCAGCCTGCA CCTGCCCCAC TGGGCACTGT TCCTTTTCGG GCTGGCCCTG 
CTCACGGCCG TCATTAACCC TTACTGGACA CACAAGAAGA CCCAGCAGCT CCTCCATCCC 
GTGGATTGGA ACTTTGCACA GGAGGAAGCC AAGGGCAGCA GGCAAGAAAG GACCAATGGC 
CAGGTGCCTC GGAAAAAGAG GTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cln8 ( XM_006508806.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508869.1 Mus musculus ceroid-lipofuscinosis, neuronal 8 (Cln8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu09299
Clone ID Related Accession (Same CDS sequence)	XM_006508806.3 , XM_006508806.4 , NM_012000.3 , XM_006508806.2 , XM_006508807.2
Accession Version	XM_006508806.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 867bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein CLN8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006508806.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTCCTG TGAGCAGCCA CGGCTTGGCG GAGAGCATTT TTGACCTGGA CTACGCTTCG 
TGGAAGATTC GGTCGACTCT AGCGGTTGCT GGCTTTGTCT TCTACCTGGG CGTCTTTGTA 
GTCTGCCATC AGCTCTCATC GTCCCTGAAT GCCACCTACC GCTCCCTGGC GGCCAAAGAG 
AAGGTCTTCT GGAACCTGGC GGCGACGCGT GCTGTCTTCG GCGTCCAGAG CACAACTGCA 
GGCCTGTGGG CCCTGCTGGG AGACCCCGTG CTCTATGCCG ACAAAGCTCT CGGGCAGCAG 
AACTGGTGTT GGTTTCACAT CACCACGGCC ACTGGCTTCT TCTTCTTTGA GAATGTGGCC 
GTTCACCTGT CCAACCTGTT CTTCCGCACC TTTGACTTGT TTCTGGTTGT CCACCACCTC 
TTTGCCTTTC TTGGGTTCCT GGGTTCAGCG ATCAATCTCA GAGCTGGCCA CTATCTAGCC 
ATGACCACGT TGCTCCTAGA GATGAGCACG CCCTTCACCT GCATTTCCTG GATGCTCCTG 
AAGGCTGGGT GGTCAGACTC CCTGTTCTGG AAGGCCAACC AGTGGCTAAT GATCCACATG 
TTTCACTGTC GAATGATCCT CACCTACCAC ATGTGGTGGG TGTGCTTCTG TCACTGGGAC 
GCCCTCACCA GCAGCCTGCA CCTGCCCCAC TGGGCACTGT TCCTTTTCGG GCTGGCCCTG 
CTCACGGCCG TCATTAACCC TTACTGGACA CACAAGAAGA CCCAGCAGCT CCTCCATCCC 
GTGGATTGGA ACTTTGCACA GGAGGAAGCC AAGGGCAGCA GGCAAGAAAG GACCAATGGC 
CAGGTGCCTC GGAAAAAGAG GTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508869.1
CDS	537..1403
Translation	MTPVSSHGLAESIFDLDYASWKIRSTLAVAGFVFYLGVFVVCHQLSSSLNATYRSLAAKEKVFWNLAATRAVFGVQSTTAGLWALLGDPVLYADKALGQQNWCWFHITTATGFFFFENVAVHLSNLFFRTFDLFLVVHHLFAFLGFLGSAINLRAGHYLAMTTLLLEMSTPFTCISWMLLKAGWSDSLFWKANQWLMIHMFHCRMILTYHMWWVCFCHWDALTSSLHLPHWALFLFGLALLTAVINPYWTHKKTQQLLHPVDWNFAQEEAKGSRQERTNGQVPRKKRL

Target ORF information:

RefSeq Version	XM_006508806.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ceroid-lipofuscinosis, neuronal 8 (Cln8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508806.3

ORF Insert Sequence:

ATGACTCCTG TGAGCAGCCA CGGCTTGGCG GAGAGCATTT TTGACCTGGA CTACGCTTCG 
TGGAAGATTC GGTCGACTCT AGCGGTTGCT GGCTTTGTCT TCTACCTGGG CGTCTTTGTA 
GTCTGCCATC AGCTCTCATC GTCCCTGAAT GCCACCTACC GCTCCCTGGC GGCCAAAGAG 
AAGGTCTTCT GGAACCTGGC GGCGACGCGT GCTGTCTTCG GCGTCCAGAG CACAACTGCA 
GGCCTGTGGG CCCTGCTGGG AGACCCCGTG CTCTATGCCG ACAAAGCTCT CGGGCAGCAG 
AACTGGTGTT GGTTTCACAT CACCACGGCC ACTGGCTTCT TCTTCTTTGA GAATGTGGCC 
GTTCACCTGT CCAACCTGTT CTTCCGCACC TTTGACTTGT TTCTGGTTGT CCACCACCTC 
TTTGCCTTTC TTGGGTTCCT GGGTTCAGCG ATCAATCTCA GAGCTGGCCA CTATCTAGCC 
ATGACCACGT TGCTCCTAGA GATGAGCACG CCCTTCACCT GCATTTCCTG GATGCTCCTG 
AAGGCTGGGT GGTCAGACTC CCTGTTCTGG AAGGCCAACC AGTGGCTAAT GATCCACATG 
TTTCACTGTC GAATGATCCT CACCTACCAC ATGTGGTGGG TGTGCTTCTG TCACTGGGAC 
GCCCTCACCA GCAGCCTGCA CCTGCCCCAC TGGGCACTGT TCCTTTTCGG GCTGGCCCTG 
CTCACGGCCG TCATTAACCC TTACTGGACA CACAAGAAGA CCCAGCAGCT CCTCCATCCC 
GTGGATTGGA ACTTTGCACA GGAGGAAGCC AAGGGCAGCA GGCAAGAAAG GACCAATGGC 
CAGGTGCCTC GGAAAAAGAG GTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cln8 ( XM_006508806.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508869.1 Mus musculus ceroid-lipofuscinosis, neuronal 8 (Cln8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu09299
Clone ID Related Accession (Same CDS sequence)	XM_006508806.3 , XM_006508806.4 , NM_012000.3 , XM_006508806.2 , XM_006508807.2
Accession Version	XM_006508806.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 867bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein CLN8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006508806.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTCCTG TGAGCAGCCA CGGCTTGGCG GAGAGCATTT TTGACCTGGA CTACGCTTCG 
TGGAAGATTC GGTCGACTCT AGCGGTTGCT GGCTTTGTCT TCTACCTGGG CGTCTTTGTA 
GTCTGCCATC AGCTCTCATC GTCCCTGAAT GCCACCTACC GCTCCCTGGC GGCCAAAGAG 
AAGGTCTTCT GGAACCTGGC GGCGACGCGT GCTGTCTTCG GCGTCCAGAG CACAACTGCA 
GGCCTGTGGG CCCTGCTGGG AGACCCCGTG CTCTATGCCG ACAAAGCTCT CGGGCAGCAG 
AACTGGTGTT GGTTTCACAT CACCACGGCC ACTGGCTTCT TCTTCTTTGA GAATGTGGCC 
GTTCACCTGT CCAACCTGTT CTTCCGCACC TTTGACTTGT TTCTGGTTGT CCACCACCTC 
TTTGCCTTTC TTGGGTTCCT GGGTTCAGCG ATCAATCTCA GAGCTGGCCA CTATCTAGCC 
ATGACCACGT TGCTCCTAGA GATGAGCACG CCCTTCACCT GCATTTCCTG GATGCTCCTG 
AAGGCTGGGT GGTCAGACTC CCTGTTCTGG AAGGCCAACC AGTGGCTAAT GATCCACATG 
TTTCACTGTC GAATGATCCT CACCTACCAC ATGTGGTGGG TGTGCTTCTG TCACTGGGAC 
GCCCTCACCA GCAGCCTGCA CCTGCCCCAC TGGGCACTGT TCCTTTTCGG GCTGGCCCTG 
CTCACGGCCG TCATTAACCC TTACTGGACA CACAAGAAGA CCCAGCAGCT CCTCCATCCC 
GTGGATTGGA ACTTTGCACA GGAGGAAGCC AAGGGCAGCA GGCAAGAAAG GACCAATGGC 
CAGGTGCCTC GGAAAAAGAG GTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508869.1
CDS	535..1401
Translation	MTPVSSHGLAESIFDLDYASWKIRSTLAVAGFVFYLGVFVVCHQLSSSLNATYRSLAAKEKVFWNLAATRAVFGVQSTTAGLWALLGDPVLYADKALGQQNWCWFHITTATGFFFFENVAVHLSNLFFRTFDLFLVVHHLFAFLGFLGSAINLRAGHYLAMTTLLLEMSTPFTCISWMLLKAGWSDSLFWKANQWLMIHMFHCRMILTYHMWWVCFCHWDALTSSLHLPHWALFLFGLALLTAVINPYWTHKKTQQLLHPVDWNFAQEEAKGSRQERTNGQVPRKKRL

Target ORF information:

RefSeq Version	XM_006508806.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus ceroid-lipofuscinosis, neuronal 8 (Cln8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508806.4

ORF Insert Sequence:

ATGACTCCTG TGAGCAGCCA CGGCTTGGCG GAGAGCATTT TTGACCTGGA CTACGCTTCG 
TGGAAGATTC GGTCGACTCT AGCGGTTGCT GGCTTTGTCT TCTACCTGGG CGTCTTTGTA 
GTCTGCCATC AGCTCTCATC GTCCCTGAAT GCCACCTACC GCTCCCTGGC GGCCAAAGAG 
AAGGTCTTCT GGAACCTGGC GGCGACGCGT GCTGTCTTCG GCGTCCAGAG CACAACTGCA 
GGCCTGTGGG CCCTGCTGGG AGACCCCGTG CTCTATGCCG ACAAAGCTCT CGGGCAGCAG 
AACTGGTGTT GGTTTCACAT CACCACGGCC ACTGGCTTCT TCTTCTTTGA GAATGTGGCC 
GTTCACCTGT CCAACCTGTT CTTCCGCACC TTTGACTTGT TTCTGGTTGT CCACCACCTC 
TTTGCCTTTC TTGGGTTCCT GGGTTCAGCG ATCAATCTCA GAGCTGGCCA CTATCTAGCC 
ATGACCACGT TGCTCCTAGA GATGAGCACG CCCTTCACCT GCATTTCCTG GATGCTCCTG 
AAGGCTGGGT GGTCAGACTC CCTGTTCTGG AAGGCCAACC AGTGGCTAAT GATCCACATG 
TTTCACTGTC GAATGATCCT CACCTACCAC ATGTGGTGGG TGTGCTTCTG TCACTGGGAC 
GCCCTCACCA GCAGCCTGCA CCTGCCCCAC TGGGCACTGT TCCTTTTCGG GCTGGCCCTG 
CTCACGGCCG TCATTAACCC TTACTGGACA CACAAGAAGA CCCAGCAGCT CCTCCATCCC 
GTGGATTGGA ACTTTGCACA GGAGGAAGCC AAGGGCAGCA GGCAAGAAAG GACCAATGGC 
CAGGTGCCTC GGAAAAAGAG GTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cln8 ( XM_006508806.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508869.1 Mus musculus ceroid-lipofuscinosis, neuronal 8 (Cln8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu09299
Clone ID Related Accession (Same CDS sequence)	XM_006508806.3 , XM_006508806.4 , NM_012000.3 , XM_006508806.2 , XM_006508807.2
Accession Version	XM_006508806.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 867bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	protein CLN8 isoform X1
Comment

ATGACTCCTG TGAGCAGCCA CGGCTTGGCG GAGAGCATTT TTGACCTGGA CTACGCTTCG 
TGGAAGATTC GGTCGACTCT AGCGGTTGCT GGCTTTGTCT TCTACCTGGG CGTCTTTGTA 
GTCTGCCATC AGCTCTCATC GTCCCTGAAT GCCACCTACC GCTCCCTGGC GGCCAAAGAG 
AAGGTCTTCT GGAACCTGGC GGCGACGCGT GCTGTCTTCG GCGTCCAGAG CACAACTGCA 
GGCCTGTGGG CCCTGCTGGG AGACCCCGTG CTCTATGCCG ACAAAGCTCT CGGGCAGCAG 
AACTGGTGTT GGTTTCACAT CACCACGGCC ACTGGCTTCT TCTTCTTTGA GAATGTGGCC 
GTTCACCTGT CCAACCTGTT CTTCCGCACC TTTGACTTGT TTCTGGTTGT CCACCACCTC 
TTTGCCTTTC TTGGGTTCCT GGGTTCAGCG ATCAATCTCA GAGCTGGCCA CTATCTAGCC 
ATGACCACGT TGCTCCTAGA GATGAGCACG CCCTTCACCT GCATTTCCTG GATGCTCCTG 
AAGGCTGGGT GGTCAGACTC CCTGTTCTGG AAGGCCAACC AGTGGCTAAT GATCCACATG 
TTTCACTGTC GAATGATCCT CACCTACCAC ATGTGGTGGG TGTGCTTCTG TCACTGGGAC 
GCCCTCACCA GCAGCCTGCA CCTGCCCCAC TGGGCACTGT TCCTTTTCGG GCTGGCCCTG 
CTCACGGCCG TCATTAACCC TTACTGGACA CACAAGAAGA CCCAGCAGCT CCTCCATCCC 
GTGGATTGGA ACTTTGCACA GGAGGAAGCC AAGGGCAGCA GGCAAGAAAG GACCAATGGC 
CAGGTGCCTC GGAAAAAGAG GTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508869.1
Translation	MTPVSSHGLAESIFDLDYASWKIRSTLAVAGFVFYLGVFVVCHQLSSSLNATYRSLAAKEKVFWNLAATRAVFGVQSTTAGLWALLGDPVLYADKALGQQNWCWFHITTATGFFFFENVAVHLSNLFFRTFDLFLVVHHLFAFLGFLGSAINLRAGHYLAMTTLLLEMSTPFTCISWMLLKAGWSDSLFWKANQWLMIHMFHCRMILTYHMWWVCFCHWDALTSSLHLPHWALFLFGLALLTAVINPYWTHKKTQQLLHPVDWNFAQEEAKGSRQERTNGQVPRKKRL

Target ORF information:

RefSeq Version	XM_006508806.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus ceroid-lipofuscinosis, neuronal 8 (Cln8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508806.2

ORF Insert Sequence:

ATGACTCCTG TGAGCAGCCA CGGCTTGGCG GAGAGCATTT TTGACCTGGA CTACGCTTCG 
TGGAAGATTC GGTCGACTCT AGCGGTTGCT GGCTTTGTCT TCTACCTGGG CGTCTTTGTA 
GTCTGCCATC AGCTCTCATC GTCCCTGAAT GCCACCTACC GCTCCCTGGC GGCCAAAGAG 
AAGGTCTTCT GGAACCTGGC GGCGACGCGT GCTGTCTTCG GCGTCCAGAG CACAACTGCA 
GGCCTGTGGG CCCTGCTGGG AGACCCCGTG CTCTATGCCG ACAAAGCTCT CGGGCAGCAG 
AACTGGTGTT GGTTTCACAT CACCACGGCC ACTGGCTTCT TCTTCTTTGA GAATGTGGCC 
GTTCACCTGT CCAACCTGTT CTTCCGCACC TTTGACTTGT TTCTGGTTGT CCACCACCTC 
TTTGCCTTTC TTGGGTTCCT GGGTTCAGCG ATCAATCTCA GAGCTGGCCA CTATCTAGCC 
ATGACCACGT TGCTCCTAGA GATGAGCACG CCCTTCACCT GCATTTCCTG GATGCTCCTG 
AAGGCTGGGT GGTCAGACTC CCTGTTCTGG AAGGCCAACC AGTGGCTAAT GATCCACATG 
TTTCACTGTC GAATGATCCT CACCTACCAC ATGTGGTGGG TGTGCTTCTG TCACTGGGAC 
GCCCTCACCA GCAGCCTGCA CCTGCCCCAC TGGGCACTGT TCCTTTTCGG GCTGGCCCTG 
CTCACGGCCG TCATTAACCC TTACTGGACA CACAAGAAGA CCCAGCAGCT CCTCCATCCC 
GTGGATTGGA ACTTTGCACA GGAGGAAGCC AAGGGCAGCA GGCAAGAAAG GACCAATGGC 
CAGGTGCCTC GGAAAAAGAG GTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cln8 ( XM_006508807.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508870.1 Mus musculus ceroid-lipofuscinosis, neuronal 8 (Cln8), transcript variant X2, mRNA.

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CloneID	OMu09299
Clone ID Related Accession (Same CDS sequence)	XM_006508806.3 , XM_006508806.4 , NM_012000.3 , XM_006508806.2 , XM_006508807.2
Accession Version	XM_006508807.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 867bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein CLN8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006508807.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTCCTG TGAGCAGCCA CGGCTTGGCG GAGAGCATTT TTGACCTGGA CTACGCTTCG 
TGGAAGATTC GGTCGACTCT AGCGGTTGCT GGCTTTGTCT TCTACCTGGG CGTCTTTGTA 
GTCTGCCATC AGCTCTCATC GTCCCTGAAT GCCACCTACC GCTCCCTGGC GGCCAAAGAG 
AAGGTCTTCT GGAACCTGGC GGCGACGCGT GCTGTCTTCG GCGTCCAGAG CACAACTGCA 
GGCCTGTGGG CCCTGCTGGG AGACCCCGTG CTCTATGCCG ACAAAGCTCT CGGGCAGCAG 
AACTGGTGTT GGTTTCACAT CACCACGGCC ACTGGCTTCT TCTTCTTTGA GAATGTGGCC 
GTTCACCTGT CCAACCTGTT CTTCCGCACC TTTGACTTGT TTCTGGTTGT CCACCACCTC 
TTTGCCTTTC TTGGGTTCCT GGGTTCAGCG ATCAATCTCA GAGCTGGCCA CTATCTAGCC 
ATGACCACGT TGCTCCTAGA GATGAGCACG CCCTTCACCT GCATTTCCTG GATGCTCCTG 
AAGGCTGGGT GGTCAGACTC CCTGTTCTGG AAGGCCAACC AGTGGCTAAT GATCCACATG 
TTTCACTGTC GAATGATCCT CACCTACCAC ATGTGGTGGG TGTGCTTCTG TCACTGGGAC 
GCCCTCACCA GCAGCCTGCA CCTGCCCCAC TGGGCACTGT TCCTTTTCGG GCTGGCCCTG 
CTCACGGCCG TCATTAACCC TTACTGGACA CACAAGAAGA CCCAGCAGCT CCTCCATCCC 
GTGGATTGGA ACTTTGCACA GGAGGAAGCC AAGGGCAGCA GGCAAGAAAG GACCAATGGC 
CAGGTGCCTC GGAAAAAGAG GTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508870.1
CDS	643..1509
Translation	MTPVSSHGLAESIFDLDYASWKIRSTLAVAGFVFYLGVFVVCHQLSSSLNATYRSLAAKEKVFWNLAATRAVFGVQSTTAGLWALLGDPVLYADKALGQQNWCWFHITTATGFFFFENVAVHLSNLFFRTFDLFLVVHHLFAFLGFLGSAINLRAGHYLAMTTLLLEMSTPFTCISWMLLKAGWSDSLFWKANQWLMIHMFHCRMILTYHMWWVCFCHWDALTSSLHLPHWALFLFGLALLTAVINPYWTHKKTQQLLHPVDWNFAQEEAKGSRQERTNGQVPRKKRL

Target ORF information:

RefSeq Version	XM_006508807.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ceroid-lipofuscinosis, neuronal 8 (Cln8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508807.2

ORF Insert Sequence:

ATGACTCCTG TGAGCAGCCA CGGCTTGGCG GAGAGCATTT TTGACCTGGA CTACGCTTCG 
TGGAAGATTC GGTCGACTCT AGCGGTTGCT GGCTTTGTCT TCTACCTGGG CGTCTTTGTA 
GTCTGCCATC AGCTCTCATC GTCCCTGAAT GCCACCTACC GCTCCCTGGC GGCCAAAGAG 
AAGGTCTTCT GGAACCTGGC GGCGACGCGT GCTGTCTTCG GCGTCCAGAG CACAACTGCA 
GGCCTGTGGG CCCTGCTGGG AGACCCCGTG CTCTATGCCG ACAAAGCTCT CGGGCAGCAG 
AACTGGTGTT GGTTTCACAT CACCACGGCC ACTGGCTTCT TCTTCTTTGA GAATGTGGCC 
GTTCACCTGT CCAACCTGTT CTTCCGCACC TTTGACTTGT TTCTGGTTGT CCACCACCTC 
TTTGCCTTTC TTGGGTTCCT GGGTTCAGCG ATCAATCTCA GAGCTGGCCA CTATCTAGCC 
ATGACCACGT TGCTCCTAGA GATGAGCACG CCCTTCACCT GCATTTCCTG GATGCTCCTG 
AAGGCTGGGT GGTCAGACTC CCTGTTCTGG AAGGCCAACC AGTGGCTAAT GATCCACATG 
TTTCACTGTC GAATGATCCT CACCTACCAC ATGTGGTGGG TGTGCTTCTG TCACTGGGAC 
GCCCTCACCA GCAGCCTGCA CCTGCCCCAC TGGGCACTGT TCCTTTTCGG GCTGGCCCTG 
CTCACGGCCG TCATTAACCC TTACTGGACA CACAAGAAGA CCCAGCAGCT CCTCCATCCC 
GTGGATTGGA ACTTTGCACA GGAGGAAGCC AAGGGCAGCA GGCAAGAAAG GACCAATGGC 
CAGGTGCCTC GGAAAAAGAG GTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

NCL disease mechanisms.
Biochimica et biophysica acta1832(11)1882-93(2013 Nov)
Palmer DN,Barry LA,Tyynel? J,Cooper JD

Identifying protein partners of CLN8, an ER-resident protein involved in neuronal ceroid lipofuscinosis.
Biochimica et biophysica acta1833(3)529-40(2013 Mar)
Passantino R,Cascio C,Deidda I,Galizzi G,Russo D,Spedale G,Guarneri P

Selective spatiotemporal patterns of glial activation and neuron loss in the sensory thalamocortical pathways of neuronal ceroid lipofuscinosis 8 mice.
Neurobiology of disease47(3)444-57(2012 Sep)
Kuronen M,Lehesjoki AE,Jalanko A,Cooper JD,Kopra O

Increased [?H]D-aspartate release and changes in glutamate receptor expression in the hippocampus of the mnd mouse.
Journal of neuroscience research90(6)1148-58(2012 Jun)
Bigini P,Milanese M,Gardoni F,Longhi A,Bonifacino T,Barbera S,Fumagalli E,Di Luca M,Mennini T,Bonanno G

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Cln8 cDNA ORF clone, Mus musculus(house mouse)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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