Casp8ap2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Casp8ap2
Entrez Gene ID	26885
Full Name	caspase 8 associated protein 2
Synonyms	D4Ertd659e,FLASH
General protein information	Preferred Names caspase 8 associated protein 2 Names CASP8-associated protein 2 FLICE-associated huge protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4 A5\|4 14.27 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006537950.1	XP_006538013.1	CASP8-associated protein 2 isoform X2
NM_001122978.2	NP_001116450.1	CASP8-associated protein 2
NM_011997.3	NP_036127.2	CASP8-associated protein 2
XM_006537950.2	XP_006538013.1	CASP8-associated protein 2 isoform X2
XM_006537949.1	XP_006538012.1	CASP8-associated protein 2 isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	CASP8AP2	XP_005210940.1
Pan troglodytes (chimpanzee)	CASP8AP2	XP_003311433.1
	CASP8AP2	XP_001098220.1
Rattus norvegicus (Norway rat)	Casp8ap2	NP_001101391.1
Mus musculus (house mouse)	Casp8ap2	NP_001116450.1
Homo sapiens (human)	CASP8AP2	NP_001131139.1
Canis lupus familiaris (dog)	CASP8AP2	XP_539042.3

Gene Ontology
Bibliography

Related articles in PubMed

Maternal expression and early induction of histone gene transcription factor Hinfp sustains development in pre-implantation embryos.
Ghule PN, Xie RL, Colby JL, Rivera-P?rez JA, Jones SN, Lian JB, Stein JL, van Wijnen AJ, Stein GS
Developmental biology419(2)311-320(2016 11)

A Novel Interaction between FLICE-Associated Huge Protein (FLASH) and E2A Regulates Cell Proliferation and Cellular Senescence via Tumor Necrosis Factor (TNF)-Alpha-p21WAF1/CIP1 Axis.
Hirano T, Murakami T, Ono H, Sakurai A, Tominaga T, Takahashi T, Nagai K, Doi T, Abe H
PloS one10(7)e0133205(2015)

FLASH/casp8ap2 is indispensable for early embryogenesis but dispensable for proliferation and differentiation of ES cells.
Minamida Y, Someda M, Yonehara S
PloS one9(9)e108032(2014)

SUMO regulates proteasome-dependent degradation of FLASH/Casp8AP2.
Vennemann A, Hofmann TG
Cell cycle (Georgetown, Tex.)12(12)1914-21(2013 Jun)

FLASH is essential during early embryogenesis and cooperates with p73 to regulate histone gene transcription.
De Cola A, Bongiorno-Borbone L, Bianchi E, Barcaroli D, Carletti E, Knight RA, Di Ilio C, Melino G, Sette C, De Laurenzi V
Oncogene31(5)573-82(2012 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the functional interaction of E2A and FLASH play an important role in cell proliferation and cellular senescence via regulation of p21 expression in experimental glomerulonephritis.
Title: A Novel Interaction between FLICE-Associated Huge Protein (FLASH) and E2A Regulates Cell Proliferation and Cellular Senescence via Tumor Necrosis Factor (TNF)-Alpha-p21WAF1/CIP1 Axis.

Data suggest that SUMO targets FLASH for proteasome-dependent degradation, which is associated with recruitment of FLASH to PML bodies.
Title: SUMO regulates proteasome-dependent degradation of FLASH/Casp8AP2.

Data show that homozygous deletion of FLASH results in early embryonic lethality.
Title: FLASH is essential during early embryogenesis and cooperates with p73 to regulate histone gene transcription.

This enzyme has an essential role in T-cell homeostasis and T-cell-mediated immunity.
Title: Essential role for caspase 8 in T-cell homeostasis and T-cell-mediated immunity.

The following Casp8ap2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Casp8ap2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu15369	NM_011997.3 Latest version!	Mus musculus caspase 8 associated protein 2 (Casp8ap2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu15369	NM_001122978.2 Latest version!	Mus musculus caspase 8 associated protein 2 (Casp8ap2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu32946	XM_006537950.2 Latest version!	Mus musculus caspase 8 associated protein 2 (Casp8ap2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu32945	XM_006537949.1 Latest version!	Mus musculus caspase 8 associated protein 2 (Casp8ap2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
View Old Accession Versions >>
OMu32946	XM_006537950.1	Mus musculus caspase 8 associated protein 2 (Casp8ap2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Casp8ap2 ( NM_001122978.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001116450.1 Mus musculus caspase 8 associated protein 2 (Casp8ap2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu15369
Clone ID Related Accession (Same CDS sequence)	NM_001122978.2 , NM_011997.3
Accession Version	NM_001122978.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Mus musculus(house mouse)
Product	CASP8-associated protein 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CA526466.1, AK142148.1 and AL805973.6. On Nov 18, 2015 this sequence version replaced NM_001122978.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AF132726.1, BC138022.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849383 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCAGCAG ATGATGACAA TGGTGATGGA ACGGGTTTGT TTGATGTCTG TCCTGCCTCT 
CCACTTAAAA ATAATGATGA AGGCTCATTG GACATATATG CTGGGTTGGA CAGTGCTGTT 
TCTGACAGTA CTGCTAGATC CTGTGTGTCA TTCAGAAACT GTTTAGATTT GTATGAAGAG 
ATCCTGACTG AAGAAGGAAC TGCGAAGGAG GCAACGTACA ATGACTTGCA GATAGAATAT 
GGGAAATGTC AGCAGCAAAT GAAAGACCTG ATGAAAAGGT TTAAGGAAAT ACAGACACAG 
AACTTGAACT TAAAAAATGA AAACCAGTCT CTTAAGAAGA ATATCTCAGC ACTTATCAAA 
ACTGCCAGAG TGGAGATAAA CCGTAAGGAT GAAGAGATAA ATCATCTTCA CCAAAGATTG 
TCTGAGTTTC CACATTTTCG AAATAACCAT AAAACTGCAA GAACAAAAGA TTCACAGTCC 
ACATCTCCCC ATTTGGATGA TTGTTCAAAG ACTGATCACG GAGTTAAAAG TGATGTTCAG 
AAAGATGTAC ATCCTAACAC TGCACAGCCA AACTTGGAAA AGGAAGGAAA ATCGCATTCT 
GAAGCACAGA ATCCTTTGCA CTTGTCTACG GGTGTTGAGA AACATTGTGC CAATAATGTC 
TGGTCACGTT CTCCTTACCA GGTTGGAGAA GGTAACTCAA ATGAGGATAA TAGGAGAGGA 
AGGAGTGGCA CTAGACATAG CCAGTGTAGC AGAGGAACTG ATAGAACACA GAAAGACTTA 
CATAGCAGCT GTAATGACAG TGAGCCAAGG GACAAGGAGG CTAACTCCAG ACTACAAGGA 
CACCCTGAGA AACATGGCAA CAGTGAAGCA AGGACGGAGA GCAAAATTTC AGAGAGTAAA 
AGCAGCACTG GTATGGGATA TAAAAGTGAG CGCAGTGCCT CTTCTTGGGA AAAAGAGACT 
TCCAGAGAAA GGCCACACAC TCGAGTGGAA TCTCAACATG ACAAAAATCT AGAGAAACAA 
AATGAAAGAT TACAAAATAT GCACAGAAAA GAGCTTCCGT CTCAGGACAA AACAGAAAGA 
AAAGTTGATG TGAAGTTTAA ACCAGCAGGA GAGGAGCAGG GGCATCGGGG AAGAGTGGAC 
CGGGCGTTAC CTCCTCATCC CAAGAATGAC GTGAAACATT ACGGCTTCAA TAAGTATCAT 
CCAGAAGAGA GAAGGGGAAG GGAAGATTGT AAAAGAGACA GAGGGATGAA CAGTCATGGC 
TTTCAAGATA GAAGATGTTC ATCTTTTCTT TCAAGCAACA GAAATAGCAA ATACCCGCAC 
TCCAAGGAAG TTAGTGTTGC ACACCAGTGG GAAAATACTC CTTTCAAAGC AGAAAGACAT 
AGAACTGAGG ACAGGAGGAA AAGAGAACGA GAAAACAAAG AAGAAAGTAG ACATGTGAAA 
AGTGACAAAA AATCACCTCC AGAACACTTA CAAAGGACTC ATAAAGACAC TAAGAAAAGC 
ACTGCTGATG GAAAGAGACA GACTGAGCCC AAACATGGTA AAGGTGCAGT CTCTAACAGT 
GAGCTTTCTA AAGGGACAGA CAGTAAAGAA GGTGCAACAA AGGTGGAGAG TGGGCCAAAT 
GAAGCAAAAG GCAAGGACTT AAAGTTAAGC TTCATGGAAA AACTGAACTT AACTCTTTCT 
CCTGCTAAAA AGCAGCCTGC TTGTCAAGAT AACCCACATC AAATAACTGG TGTTCCCGAG 
CCCAGTGGCA CGTGTGACTC ACGGTCCTTG GAGACGACTG GAACGGTGGC ATGCCTTCCT 
TCTGGCAGTG AACATAATAG AGAGGAAACC AAATCAGAGT TACCAGAGCC AAAGGAGGCT 
CTTTTGGCAA CATCTCAACT CAGGATCAGC ATTCCAGAAA ACAAAATGAA GGAAGAAAAG 
AGGTTGTTAT TTAAATCTGT TGAGAATACT GTGCCTTGTG AACTGCTTGC TTGTGGCACG 
GAAATTTCCC TCCCAGCACC TGTAGAAATT GAACAAGCAA GATGCTTGCT TGGATCAGTG 
GAAGTGGAAG AGACCTGTGG TGGTGCAAGG ACAGCCGCTT CTGTGGTGAT GCATGTGTTA 
CCAGAACATG CTTCTGAAGA TGCCAGCCAA GAATTGGACA CCAAAAGACA CGATGGTATA 
AATGCTTGTG CTATTTCTGA AGGTGTGAAA ACAAAGGTGA TTCTTTCACC AAAAGCAGCT 
GCAGCCAGTG AGAGCCATCT TGCACCTTTG GTTGAAGAAC CTAGCATTTC ACTAGTAAAC 
TGTTCGGGAG ACAATAATCC TAAACTTGAA CCTTCTCTTG AAGAGAGGCC TATAGTTGAG 
ACTAAATCCT GTCCTTTGGA GTCTTGTTTA CCTAAAGAGA CTTTTGTACC TTCACCACAG 
AAGACTGAGT TGATTGACCA CAAAATAGAA ACTGGAGAAT CAAACTCAGT ATATCAAGAT 
GATGATAACT CAGTTTTGAG TATTGACTTT AATAATCTGA GACCTATTCC AGACCCCATC 
AGCCCTCTGA ATAGTCCAGT GAGACCGGTA TGCAAAGTTG TTAGCATGGA GAGCTCATGT 
GCAATTCCAC TGTATGATAG CAGTCATAAA GATGAGTTTC CATCCAATTC AACTCTTTCT 
ACCTTCAAGA GTCAGTCTGA TCTAAATAAG GAAAATGAAA AACCAGTTCC CAAATTTGAC 
AAATGTTCAG AAGCAGATTC TTGTAAGCAT TTGTCTTTAG ATGAATTAGA AGAAGGCGAA 
ATTAGAAGTG ATGATGAAGA ATCTGTAGCA CAAAAACGCT TGGAAAAGAG TGCAAGACCT 
AGAGTTTCTG CTGAAGTGCA GCCAGGGAAA AGCAGCCCAG GAAGCAGGAG GAGCACTGTG 
CATGTGCATA AGGACAACGG GAGGACTGCT GTAAAACTCC CTCGGGACAG ACTCACATGG 
AGCAAAAGAT CAAGTGAATC AAGACCCTCC AACACCGAAA GGAAAAGTAA AACAATGAGC 
ATCTCCAGCT TGGAAAAAAT ACTTCCGCTT ATCCTTGTAC CCTCTTCCCT GTGGGAGGTT 
ATGCATATGT TACGGTTGCT AGGTAAACAT GTAAGAAAAA ATTATATGAA ATTCAAGATA 
AAATTTTCAT TGACACAATT TCATAGAATT ATTGAATCTG CAATTTTGAG TTTCACATCA 
CTAGTTAAAT GCCTTGATTT GTCTAAGATC TGTAAGTCAG TAAGTACTTT ACAAAAGAGT 
CTTTGTGAAG TTATAGAATC TAACCTTAAA CAAGTGAAGA AGAATGGCAT AGTTGACCGT 
TTATTTGAAC AGCAACAAAC AGATATGAAA AAAAAATTGT GGAAGTTTGT AGATGAACAA 
CTTGATTATT TGTTTGAAAA GCTTAAAAAA ATCCTACTAA AGTTTTGTGA TTCTGTAAAC 
TTTGAAAATG AGAATAGTGA AGGAAAACTT GGAAAAAAAT ATAAAGAGAG AACCCAACAT 
TCAAATTGTC AGAAGAAAAA AATGGACAAC AAAGAAATAC GGAGAGAAAA AGTGCTAAAA 
TCAGAAAATA CTGTGAATTT TAAATCTTCA CTGGGATGTG AAAAATCTGA AGAAAAACAT 
CAGGACCAAA ATAAAACCAA TGCTAGTATA GTAAAGCATG ATGTAAAAAG AACTTTTAGC 
ACTTGCAGTG ATAATACAAA GAACGCTGAA TGTAAAGAGC AGTTTCTGGA AAAGAGCTGC 
CCGAGTACCC CTAGGCCAGG AAAAGATGAA GGACATACTG AGGAGGAGGC ACAGGCGGCA 
CAGCATGCAA GTGCTAAGTC CGAACGGAGC TTTGAGATCC TTACTGAGCA GCAGGCATCC 
AGCCTTACTT TTAACTTAGT GAGTGATGCA CAGATGGGTG AGATATTTAA AAGCTTGCTA 
CAAGGTTCTG ATCTGTTGGA CACAAGTGGC ACTGAAAAGG CAGAGTGGGA ATTAAAGACT 
CCAGAGAAAC AGCTGCTGGA AAGCCTCAAG TGTGAATCTG CACCAGCTTG TGCAACAGAA 
GAGCTGGTTT CAGAGGGGGC TTCTCTTTGT CCAAAAGTGA TCAGTGATGA TAATTGGTCT 
TTATTATCAT CTGAAAAGGG TCCATCTTTA TCTTCAGGGC TTTCACTGCC AGTTCATCCT 
GATGTGTTAG ATGAAAATTG TATGTTTGAA GTATCTTCTA ACACTGCTTT AGGTAAAGAT 
AATGTATACA GCTCAGAAAA GAGTAAGCCC TGCATCTCTT CCATACTCTT AGAAGATCTT 
GCGGTCTCTT TAACAGTACC GTCACCTCTG AAATCAGATG GCCATTTGAG TTTCTTAAAG 
CCAGAAGTTT TGTCAACTTC AACTCCTGAA GAAGTTATTA GTGCACATTT TAGTGAGGAT 
GCTTTGCTTG AGGAAGAGGA TGCATCTGAA CAGGACATTC ATCTAGCTCT GGAGTCTGAT 
AACTCAAGCA GTAAGTCAAG CTGTTCATCA TGGACAAGCC GGTCTGTTGC TTCAGGCTTT 
CAGTACCACC CTAATCTTCC CATGCATGCT GTCATAATGG AAAAGTCCAA TGATCATTTC 
ATTGTGAAAA TACGGCGTGC AACACCATCT ACCTCCCCTG GCCTTAAACA TGGTGTGGTA 
GCTGAGGAGT CATTGACATC TTTGCCTAGA ACTGGAAAAG AAGCTGGTGT AGCAACAGAG 
AAAGAACCTA ACCTGTTTCA GAGTACAGTT TTAAAACCTG TCAAGGACTT GGAAAATACT 
GATAAAAATA TTGATAAGAG TAAACTAACT CATGAAGAAC AGAACTCTAT AGTACAAACA 
CAGGTTCCAG ATATATATGA ATTTCTTAAA GATGCCTCAA ATAAGGTGGT TCATTGTGAT 
CAAGTGGTTG ATGATTGCTT CAAGTTGCAT CAAGTATGGG AACCAAAAGT TTCTGAGAAC 
CTTCAAGAGT TGCCTTCAAT GGAGAAAATC CCACACTCTC TTGATAATCA TCTTCCTGAT 
ACACACATAG ATCTAACGAA AGATTCAGCC ACTGAGACCA AAAGCTTGGG GGAGCTAATG 
GAAGTAACAG TTTTAAATGT TGATCACTTG GAATGTTCTC AAACCAACTT AGATCAAGAT 
GCAGAGATAA CATGTAGTTC TTTACAGCCT GATACTATAG ATGCTTTTAT TGATTTGACA 
CATGATGCTT CAAGTGAGAG TAAAAATGAA GGTAGTGAAC CTGTGTTAGC TGTTGAAGGT 
ATGGGATGCC AGGTAATATG TATAGATGAG GATACCAACA AAGAAGGAAA GATGGGAAGG 
GCAAACAGTC CTTTAGAAAG TATTGTTGAA GAAACTTGTA TTGATTTGAC CTCAGAGTCT 
CCTGGCTCCT GTGAAATCAA GAGACATAAT TTAAAGTCGG AGCCTCCATC AAAGTTGGAT 
TGTTTAGAGT TGCCTGAAAC TCTGGGTAAT GGTCACAAGA AGAGGAAAAA CAGTCCTGGT 
GTTAGTCACT CTTCTCAGAA AAAACAAAGA AAGGACATAG ACTTAAGTAG TGAAAAGACC 
CAGAGACTTA GTCCCAATTC TGATAGAAAT GGTGATGCTC ACAGAAAGCA AGCGAGCAAG 
AAAAGAGAAC CTGCAGTAAA TGAAACGTCC TTGTCATCAG AGGCCAGCCC AGAGGTGAAG 
GGTTCAACAG CAGTACTTGC TGCTTCCCCA GCAAGCCTTT CTGCAAAAAA TGTTATCAAA 
AAGAAGGGAG AAATTATAGT TTCATGGACA AGAAATGATG ACCGGGAAAT TTTACTGGAA 
TGTCAGAAAA GAATGCCGTC CCTGAAAACA TTTACTTATT TAGCTGTCAA GCTGAATAAA 
AATCCAAATC AGGTTTCAGA GAGGTTCCAG CAGCTGAAAA AGCTCTTTGA GAAGTCAAAA 
TGCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001116450.1
CDS	52..5940
Translation	MAADDDNGDGTGLFDVCPASPLKNNDEGSLDIYAGLDSAVSDSTARSCVSFRNCLDLYEEILTEEGTAKEATYNDLQIEYGKCQQQMKDLMKRFKEIQTQNLNLKNENQSLKKNISALIKTARVEINRKDEEINHLHQRLSEFPHFRNNHKTARTKDSQSTSPHLDDCSKTDHGVKSDVQKDVHPNTAQPNLEKEGKSHSEAQNPLHLSTGVEKHCANNVWSRSPYQVGEGNSNEDNRRGRSGTRHSQCSRGTDRTQKDLHSSCNDSEPRDKEANSRLQGHPEKHGNSEARTESKISESKSSTGMGYKSERSASSWEKETSRERPHTRVESQHDKNLEKQNERLQNMHRKELPSQDKTERKVDVKFKPAGEEQGHRGRVDRALPPHPKNDVKHYGFNKYHPEERRGREDCKRDRGMNSHGFQDRRCSSFLSSNRNSKYPHSKEVSVAHQWENTPFKAERHRTEDRRKRERENKEESRHVKSDKKSPPEHLQRTHKDTKKSTADGKRQTEPKHGKGAVSNSELSKGTDSKEGATKVESGPNEAKGKDLKLSFMEKLNLTLSPAKKQPACQDNPHQITGVPEPSGTCDSRSLETTGTVACLPSGSEHNREETKSELPEPKEALLATSQLRISIPENKMKEEKRLLFKSVENTVPCELLACGTEISLPAPVEIEQARCLLGSVEVEETCGGARTAASVVMHVLPEHASEDASQELDTKRHDGINACAISEGVKTKVILSPKAAAASESHLAPLVEEPSISLVNCSGDNNPKLEPSLEERPIVETKSCPLESCLPKETFVPSPQKTELIDHKIETGESNSVYQDDDNSVLSIDFNNLRPIPDPISPLNSPVRPVCKVVSMESSCAIPLYDSSHKDEFPSNSTLSTFKSQSDLNKENEKPVPKFDKCSEADSCKHLSLDELEEGEIRSDDEESVAQKRLEKSARPRVSAEVQPGKSSPGSRRSTVHVHKDNGRTAVKLPRDRLTWSKRSSESRPSNTERKSKTMSISSLEKILPLILVPSSLWEVMHMLRLLGKHVRKNYMKFKIKFSLTQFHRIIESAILSFTSLVKCLDLSKICKSVSTLQKSLCEVIESNLKQVKKNGIVDRLFEQQQTDMKKKLWKFVDEQLDYLFEKLKKILLKFCDSVNFENENSEGKLGKKYKERTQHSNCQKKKMDNKEIRREKVLKSENTVNFKSSLGCEKSEEKHQDQNKTNASIVKHDVKRTFSTCSDNTKNAECKEQFLEKSCPSTPRPGKDEGHTEEEAQAAQHASAKSERSFEILTEQQASSLTFNLVSDAQMGEIFKSLLQGSDLLDTSGTEKAEWELKTPEKQLLESLKCESAPACATEELVSEGASLCPKVISDDNWSLLSSEKGPSLSSGLSLPVHPDVLDENCMFEVSSNTALGKDNVYSSEKSKPCISSILLEDLAVSLTVPSPLKSDGHLSFLKPEVLSTSTPEEVISAHFSEDALLEEEDASEQDIHLALESDNSSSKSSCSSWTSRSVASGFQYHPNLPMHAVIMEKSNDHFIVKIRRATPSTSPGLKHGVVAEESLTSLPRTGKEAGVATEKEPNLFQSTVLKPVKDLENTDKNIDKSKLTHEEQNSIVQTQVPDIYEFLKDASNKVVHCDQVVDDCFKLHQVWEPKVSENLQELPSMEKIPHSLDNHLPDTHIDLTKDSATETKSLGELMEVTVLNVDHLECSQTNLDQDAEITCSSLQPDTIDAFIDLTHDASSESKNEGSEPVLAVEGMGCQVICIDEDTNKEGKMGRANSPLESIVEETCIDLTSESPGSCEIKRHNLKSEPPSKLDCLELPETLGNGHKKRKNSPGVSHSSQKKQRKDIDLSSEKTQRLSPNSDRNGDAHRKQASKKREPAVNETSLSSEASPEVKGSTAVLAASPASLSAKNVIKKKGEIIVSWTRNDDREILLECQKRMPSLKTFTYLAVKLNKNPNQVSERFQQLKKLFEKSKCR

Target ORF information:

RefSeq Version	NM_001122978.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus caspase 8 associated protein 2 (Casp8ap2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001122978.2

ORF Insert Sequence:

ATGGCAGCAG ATGATGACAA TGGTGATGGA ACGGGTTTGT TTGATGTCTG TCCTGCCTCT 
CCACTTAAAA ATAATGATGA AGGCTCATTG GACATATATG CTGGGTTGGA CAGTGCTGTT 
TCTGACAGTA CTGCTAGATC CTGTGTGTCA TTCAGAAACT GTTTAGATTT GTATGAAGAG 
ATCCTGACTG AAGAAGGAAC TGCGAAGGAG GCAACGTACA ATGACTTGCA GATAGAATAT 
GGGAAATGTC AGCAGCAAAT GAAAGACCTG ATGAAAAGGT TTAAGGAAAT ACAGACACAG 
AACTTGAACT TAAAAAATGA AAACCAGTCT CTTAAGAAGA ATATCTCAGC ACTTATCAAA 
ACTGCCAGAG TGGAGATAAA CCGTAAGGAT GAAGAGATAA ATCATCTTCA CCAAAGATTG 
TCTGAGTTTC CACATTTTCG AAATAACCAT AAAACTGCAA GAACAAAAGA TTCACAGTCC 
ACATCTCCCC ATTTGGATGA TTGTTCAAAG ACTGATCACG GAGTTAAAAG TGATGTTCAG 
AAAGATGTAC ATCCTAACAC TGCACAGCCA AACTTGGAAA AGGAAGGAAA ATCGCATTCT 
GAAGCACAGA ATCCTTTGCA CTTGTCTACG GGTGTTGAGA AACATTGTGC CAATAATGTC 
TGGTCACGTT CTCCTTACCA GGTTGGAGAA GGTAACTCAA ATGAGGATAA TAGGAGAGGA 
AGGAGTGGCA CTAGACATAG CCAGTGTAGC AGAGGAACTG ATAGAACACA GAAAGACTTA 
CATAGCAGCT GTAATGACAG TGAGCCAAGG GACAAGGAGG CTAACTCCAG ACTACAAGGA 
CACCCTGAGA AACATGGCAA CAGTGAAGCA AGGACGGAGA GCAAAATTTC AGAGAGTAAA 
AGCAGCACTG GTATGGGATA TAAAAGTGAG CGCAGTGCCT CTTCTTGGGA AAAAGAGACT 
TCCAGAGAAA GGCCACACAC TCGAGTGGAA TCTCAACATG ACAAAAATCT AGAGAAACAA 
AATGAAAGAT TACAAAATAT GCACAGAAAA GAGCTTCCGT CTCAGGACAA AACAGAAAGA 
AAAGTTGATG TGAAGTTTAA ACCAGCAGGA GAGGAGCAGG GGCATCGGGG AAGAGTGGAC 
CGGGCGTTAC CTCCTCATCC CAAGAATGAC GTGAAACATT ACGGCTTCAA TAAGTATCAT 
CCAGAAGAGA GAAGGGGAAG GGAAGATTGT AAAAGAGACA GAGGGATGAA CAGTCATGGC 
TTTCAAGATA GAAGATGTTC ATCTTTTCTT TCAAGCAACA GAAATAGCAA ATACCCGCAC 
TCCAAGGAAG TTAGTGTTGC ACACCAGTGG GAAAATACTC CTTTCAAAGC AGAAAGACAT 
AGAACTGAGG ACAGGAGGAA AAGAGAACGA GAAAACAAAG AAGAAAGTAG ACATGTGAAA 
AGTGACAAAA AATCACCTCC AGAACACTTA CAAAGGACTC ATAAAGACAC TAAGAAAAGC 
ACTGCTGATG GAAAGAGACA GACTGAGCCC AAACATGGTA AAGGTGCAGT CTCTAACAGT 
GAGCTTTCTA AAGGGACAGA CAGTAAAGAA GGTGCAACAA AGGTGGAGAG TGGGCCAAAT 
GAAGCAAAAG GCAAGGACTT AAAGTTAAGC TTCATGGAAA AACTGAACTT AACTCTTTCT 
CCTGCTAAAA AGCAGCCTGC TTGTCAAGAT AACCCACATC AAATAACTGG TGTTCCCGAG 
CCCAGTGGCA CGTGTGACTC ACGGTCCTTG GAGACGACTG GAACGGTGGC ATGCCTTCCT 
TCTGGCAGTG AACATAATAG AGAGGAAACC AAATCAGAGT TACCAGAGCC AAAGGAGGCT 
CTTTTGGCAA CATCTCAACT CAGGATCAGC ATTCCAGAAA ACAAAATGAA GGAAGAAAAG 
AGGTTGTTAT TTAAATCTGT TGAGAATACT GTGCCTTGTG AACTGCTTGC TTGTGGCACG 
GAAATTTCCC TCCCAGCACC TGTAGAAATT GAACAAGCAA GATGCTTGCT TGGATCAGTG 
GAAGTGGAAG AGACCTGTGG TGGTGCAAGG ACAGCCGCTT CTGTGGTGAT GCATGTGTTA 
CCAGAACATG CTTCTGAAGA TGCCAGCCAA GAATTGGACA CCAAAAGACA CGATGGTATA 
AATGCTTGTG CTATTTCTGA AGGTGTGAAA ACAAAGGTGA TTCTTTCACC AAAAGCAGCT 
GCAGCCAGTG AGAGCCATCT TGCACCTTTG GTTGAAGAAC CTAGCATTTC ACTAGTAAAC 
TGTTCGGGAG ACAATAATCC TAAACTTGAA CCTTCTCTTG AAGAGAGGCC TATAGTTGAG 
ACTAAATCCT GTCCTTTGGA GTCTTGTTTA CCTAAAGAGA CTTTTGTACC TTCACCACAG 
AAGACTGAGT TGATTGACCA CAAAATAGAA ACTGGAGAAT CAAACTCAGT ATATCAAGAT 
GATGATAACT CAGTTTTGAG TATTGACTTT AATAATCTGA GACCTATTCC AGACCCCATC 
AGCCCTCTGA ATAGTCCAGT GAGACCGGTA TGCAAAGTTG TTAGCATGGA GAGCTCATGT 
GCAATTCCAC TGTATGATAG CAGTCATAAA GATGAGTTTC CATCCAATTC AACTCTTTCT 
ACCTTCAAGA GTCAGTCTGA TCTAAATAAG GAAAATGAAA AACCAGTTCC CAAATTTGAC 
AAATGTTCAG AAGCAGATTC TTGTAAGCAT TTGTCTTTAG ATGAATTAGA AGAAGGCGAA 
ATTAGAAGTG ATGATGAAGA ATCTGTAGCA CAAAAACGCT TGGAAAAGAG TGCAAGACCT 
AGAGTTTCTG CTGAAGTGCA GCCAGGGAAA AGCAGCCCAG GAAGCAGGAG GAGCACTGTG 
CATGTGCATA AGGACAACGG GAGGACTGCT GTAAAACTCC CTCGGGACAG ACTCACATGG 
AGCAAAAGAT CAAGTGAATC AAGACCCTCC AACACCGAAA GGAAAAGTAA AACAATGAGC 
ATCTCCAGCT TGGAAAAAAT ACTTCCGCTT ATCCTTGTAC CCTCTTCCCT GTGGGAGGTT 
ATGCATATGT TACGGTTGCT AGGTAAACAT GTAAGAAAAA ATTATATGAA ATTCAAGATA 
AAATTTTCAT TGACACAATT TCATAGAATT ATTGAATCTG CAATTTTGAG TTTCACATCA 
CTAGTTAAAT GCCTTGATTT GTCTAAGATC TGTAAGTCAG TAAGTACTTT ACAAAAGAGT 
CTTTGTGAAG TTATAGAATC TAACCTTAAA CAAGTGAAGA AGAATGGCAT AGTTGACCGT 
TTATTTGAAC AGCAACAAAC AGATATGAAA AAAAAATTGT GGAAGTTTGT AGATGAACAA 
CTTGATTATT TGTTTGAAAA GCTTAAAAAA ATCCTACTAA AGTTTTGTGA TTCTGTAAAC 
TTTGAAAATG AGAATAGTGA AGGAAAACTT GGAAAAAAAT ATAAAGAGAG AACCCAACAT 
TCAAATTGTC AGAAGAAAAA AATGGACAAC AAAGAAATAC GGAGAGAAAA AGTGCTAAAA 
TCAGAAAATA CTGTGAATTT TAAATCTTCA CTGGGATGTG AAAAATCTGA AGAAAAACAT 
CAGGACCAAA ATAAAACCAA TGCTAGTATA GTAAAGCATG ATGTAAAAAG AACTTTTAGC 
ACTTGCAGTG ATAATACAAA GAACGCTGAA TGTAAAGAGC AGTTTCTGGA AAAGAGCTGC 
CCGAGTACCC CTAGGCCAGG AAAAGATGAA GGACATACTG AGGAGGAGGC ACAGGCGGCA 
CAGCATGCAA GTGCTAAGTC CGAACGGAGC TTTGAGATCC TTACTGAGCA GCAGGCATCC 
AGCCTTACTT TTAACTTAGT GAGTGATGCA CAGATGGGTG AGATATTTAA AAGCTTGCTA 
CAAGGTTCTG ATCTGTTGGA CACAAGTGGC ACTGAAAAGG CAGAGTGGGA ATTAAAGACT 
CCAGAGAAAC AGCTGCTGGA AAGCCTCAAG TGTGAATCTG CACCAGCTTG TGCAACAGAA 
GAGCTGGTTT CAGAGGGGGC TTCTCTTTGT CCAAAAGTGA TCAGTGATGA TAATTGGTCT 
TTATTATCAT CTGAAAAGGG TCCATCTTTA TCTTCAGGGC TTTCACTGCC AGTTCATCCT 
GATGTGTTAG ATGAAAATTG TATGTTTGAA GTATCTTCTA ACACTGCTTT AGGTAAAGAT 
AATGTATACA GCTCAGAAAA GAGTAAGCCC TGCATCTCTT CCATACTCTT AGAAGATCTT 
GCGGTCTCTT TAACAGTACC GTCACCTCTG AAATCAGATG GCCATTTGAG TTTCTTAAAG 
CCAGAAGTTT TGTCAACTTC AACTCCTGAA GAAGTTATTA GTGCACATTT TAGTGAGGAT 
GCTTTGCTTG AGGAAGAGGA TGCATCTGAA CAGGACATTC ATCTAGCTCT GGAGTCTGAT 
AACTCAAGCA GTAAGTCAAG CTGTTCATCA TGGACAAGCC GGTCTGTTGC TTCAGGCTTT 
CAGTACCACC CTAATCTTCC CATGCATGCT GTCATAATGG AAAAGTCCAA TGATCATTTC 
ATTGTGAAAA TACGGCGTGC AACACCATCT ACCTCCCCTG GCCTTAAACA TGGTGTGGTA 
GCTGAGGAGT CATTGACATC TTTGCCTAGA ACTGGAAAAG AAGCTGGTGT AGCAACAGAG 
AAAGAACCTA ACCTGTTTCA GAGTACAGTT TTAAAACCTG TCAAGGACTT GGAAAATACT 
GATAAAAATA TTGATAAGAG TAAACTAACT CATGAAGAAC AGAACTCTAT AGTACAAACA 
CAGGTTCCAG ATATATATGA ATTTCTTAAA GATGCCTCAA ATAAGGTGGT TCATTGTGAT 
CAAGTGGTTG ATGATTGCTT CAAGTTGCAT CAAGTATGGG AACCAAAAGT TTCTGAGAAC 
CTTCAAGAGT TGCCTTCAAT GGAGAAAATC CCACACTCTC TTGATAATCA TCTTCCTGAT 
ACACACATAG ATCTAACGAA AGATTCAGCC ACTGAGACCA AAAGCTTGGG GGAGCTAATG 
GAAGTAACAG TTTTAAATGT TGATCACTTG GAATGTTCTC AAACCAACTT AGATCAAGAT 
GCAGAGATAA CATGTAGTTC TTTACAGCCT GATACTATAG ATGCTTTTAT TGATTTGACA 
CATGATGCTT CAAGTGAGAG TAAAAATGAA GGTAGTGAAC CTGTGTTAGC TGTTGAAGGT 
ATGGGATGCC AGGTAATATG TATAGATGAG GATACCAACA AAGAAGGAAA GATGGGAAGG 
GCAAACAGTC CTTTAGAAAG TATTGTTGAA GAAACTTGTA TTGATTTGAC CTCAGAGTCT 
CCTGGCTCCT GTGAAATCAA GAGACATAAT TTAAAGTCGG AGCCTCCATC AAAGTTGGAT 
TGTTTAGAGT TGCCTGAAAC TCTGGGTAAT GGTCACAAGA AGAGGAAAAA CAGTCCTGGT 
GTTAGTCACT CTTCTCAGAA AAAACAAAGA AAGGACATAG ACTTAAGTAG TGAAAAGACC 
CAGAGACTTA GTCCCAATTC TGATAGAAAT GGTGATGCTC ACAGAAAGCA AGCGAGCAAG 
AAAAGAGAAC CTGCAGTAAA TGAAACGTCC TTGTCATCAG AGGCCAGCCC AGAGGTGAAG 
GGTTCAACAG CAGTACTTGC TGCTTCCCCA GCAAGCCTTT CTGCAAAAAA TGTTATCAAA 
AAGAAGGGAG AAATTATAGT TTCATGGACA AGAAATGATG ACCGGGAAAT TTTACTGGAA 
TGTCAGAAAA GAATGCCGTC CCTGAAAACA TTTACTTATT TAGCTGTCAA GCTGAATAAA 
AATCCAAATC AGGTTTCAGA GAGGTTCCAG CAGCTGAAAA AGCTCTTTGA GAAGTCAAAA 
TGCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Casp8ap2 ( NM_011997.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_036127.2 Mus musculus caspase 8 associated protein 2 (Casp8ap2), transcript variant 1, mRNA.

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CloneID	OMu15369
Clone ID Related Accession (Same CDS sequence)	NM_001122978.2 , NM_011997.3
Accession Version	NM_011997.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Mus musculus(house mouse)
Product	CASP8-associated protein 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL954737.5 and AL805973.6. On Nov 18, 2015 this sequence version replaced NM_011997.2. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF132726.1, BC138022.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849383 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGCAG ATGATGACAA TGGTGATGGA ACGGGTTTGT TTGATGTCTG TCCTGCCTCT 
CCACTTAAAA ATAATGATGA AGGCTCATTG GACATATATG CTGGGTTGGA CAGTGCTGTT 
TCTGACAGTA CTGCTAGATC CTGTGTGTCA TTCAGAAACT GTTTAGATTT GTATGAAGAG 
ATCCTGACTG AAGAAGGAAC TGCGAAGGAG GCAACGTACA ATGACTTGCA GATAGAATAT 
GGGAAATGTC AGCAGCAAAT GAAAGACCTG ATGAAAAGGT TTAAGGAAAT ACAGACACAG 
AACTTGAACT TAAAAAATGA AAACCAGTCT CTTAAGAAGA ATATCTCAGC ACTTATCAAA 
ACTGCCAGAG TGGAGATAAA CCGTAAGGAT GAAGAGATAA ATCATCTTCA CCAAAGATTG 
TCTGAGTTTC CACATTTTCG AAATAACCAT AAAACTGCAA GAACAAAAGA TTCACAGTCC 
ACATCTCCCC ATTTGGATGA TTGTTCAAAG ACTGATCACG GAGTTAAAAG TGATGTTCAG 
AAAGATGTAC ATCCTAACAC TGCACAGCCA AACTTGGAAA AGGAAGGAAA ATCGCATTCT 
GAAGCACAGA ATCCTTTGCA CTTGTCTACG GGTGTTGAGA AACATTGTGC CAATAATGTC 
TGGTCACGTT CTCCTTACCA GGTTGGAGAA GGTAACTCAA ATGAGGATAA TAGGAGAGGA 
AGGAGTGGCA CTAGACATAG CCAGTGTAGC AGAGGAACTG ATAGAACACA GAAAGACTTA 
CATAGCAGCT GTAATGACAG TGAGCCAAGG GACAAGGAGG CTAACTCCAG ACTACAAGGA 
CACCCTGAGA AACATGGCAA CAGTGAAGCA AGGACGGAGA GCAAAATTTC AGAGAGTAAA 
AGCAGCACTG GTATGGGATA TAAAAGTGAG CGCAGTGCCT CTTCTTGGGA AAAAGAGACT 
TCCAGAGAAA GGCCACACAC TCGAGTGGAA TCTCAACATG ACAAAAATCT AGAGAAACAA 
AATGAAAGAT TACAAAATAT GCACAGAAAA GAGCTTCCGT CTCAGGACAA AACAGAAAGA 
AAAGTTGATG TGAAGTTTAA ACCAGCAGGA GAGGAGCAGG GGCATCGGGG AAGAGTGGAC 
CGGGCGTTAC CTCCTCATCC CAAGAATGAC GTGAAACATT ACGGCTTCAA TAAGTATCAT 
CCAGAAGAGA GAAGGGGAAG GGAAGATTGT AAAAGAGACA GAGGGATGAA CAGTCATGGC 
TTTCAAGATA GAAGATGTTC ATCTTTTCTT TCAAGCAACA GAAATAGCAA ATACCCGCAC 
TCCAAGGAAG TTAGTGTTGC ACACCAGTGG GAAAATACTC CTTTCAAAGC AGAAAGACAT 
AGAACTGAGG ACAGGAGGAA AAGAGAACGA GAAAACAAAG AAGAAAGTAG ACATGTGAAA 
AGTGACAAAA AATCACCTCC AGAACACTTA CAAAGGACTC ATAAAGACAC TAAGAAAAGC 
ACTGCTGATG GAAAGAGACA GACTGAGCCC AAACATGGTA AAGGTGCAGT CTCTAACAGT 
GAGCTTTCTA AAGGGACAGA CAGTAAAGAA GGTGCAACAA AGGTGGAGAG TGGGCCAAAT 
GAAGCAAAAG GCAAGGACTT AAAGTTAAGC TTCATGGAAA AACTGAACTT AACTCTTTCT 
CCTGCTAAAA AGCAGCCTGC TTGTCAAGAT AACCCACATC AAATAACTGG TGTTCCCGAG 
CCCAGTGGCA CGTGTGACTC ACGGTCCTTG GAGACGACTG GAACGGTGGC ATGCCTTCCT 
TCTGGCAGTG AACATAATAG AGAGGAAACC AAATCAGAGT TACCAGAGCC AAAGGAGGCT 
CTTTTGGCAA CATCTCAACT CAGGATCAGC ATTCCAGAAA ACAAAATGAA GGAAGAAAAG 
AGGTTGTTAT TTAAATCTGT TGAGAATACT GTGCCTTGTG AACTGCTTGC TTGTGGCACG 
GAAATTTCCC TCCCAGCACC TGTAGAAATT GAACAAGCAA GATGCTTGCT TGGATCAGTG 
GAAGTGGAAG AGACCTGTGG TGGTGCAAGG ACAGCCGCTT CTGTGGTGAT GCATGTGTTA 
CCAGAACATG CTTCTGAAGA TGCCAGCCAA GAATTGGACA CCAAAAGACA CGATGGTATA 
AATGCTTGTG CTATTTCTGA AGGTGTGAAA ACAAAGGTGA TTCTTTCACC AAAAGCAGCT 
GCAGCCAGTG AGAGCCATCT TGCACCTTTG GTTGAAGAAC CTAGCATTTC ACTAGTAAAC 
TGTTCGGGAG ACAATAATCC TAAACTTGAA CCTTCTCTTG AAGAGAGGCC TATAGTTGAG 
ACTAAATCCT GTCCTTTGGA GTCTTGTTTA CCTAAAGAGA CTTTTGTACC TTCACCACAG 
AAGACTGAGT TGATTGACCA CAAAATAGAA ACTGGAGAAT CAAACTCAGT ATATCAAGAT 
GATGATAACT CAGTTTTGAG TATTGACTTT AATAATCTGA GACCTATTCC AGACCCCATC 
AGCCCTCTGA ATAGTCCAGT GAGACCGGTA TGCAAAGTTG TTAGCATGGA GAGCTCATGT 
GCAATTCCAC TGTATGATAG CAGTCATAAA GATGAGTTTC CATCCAATTC AACTCTTTCT 
ACCTTCAAGA GTCAGTCTGA TCTAAATAAG GAAAATGAAA AACCAGTTCC CAAATTTGAC 
AAATGTTCAG AAGCAGATTC TTGTAAGCAT TTGTCTTTAG ATGAATTAGA AGAAGGCGAA 
ATTAGAAGTG ATGATGAAGA ATCTGTAGCA CAAAAACGCT TGGAAAAGAG TGCAAGACCT 
AGAGTTTCTG CTGAAGTGCA GCCAGGGAAA AGCAGCCCAG GAAGCAGGAG GAGCACTGTG 
CATGTGCATA AGGACAACGG GAGGACTGCT GTAAAACTCC CTCGGGACAG ACTCACATGG 
AGCAAAAGAT CAAGTGAATC AAGACCCTCC AACACCGAAA GGAAAAGTAA AACAATGAGC 
ATCTCCAGCT TGGAAAAAAT ACTTCCGCTT ATCCTTGTAC CCTCTTCCCT GTGGGAGGTT 
ATGCATATGT TACGGTTGCT AGGTAAACAT GTAAGAAAAA ATTATATGAA ATTCAAGATA 
AAATTTTCAT TGACACAATT TCATAGAATT ATTGAATCTG CAATTTTGAG TTTCACATCA 
CTAGTTAAAT GCCTTGATTT GTCTAAGATC TGTAAGTCAG TAAGTACTTT ACAAAAGAGT 
CTTTGTGAAG TTATAGAATC TAACCTTAAA CAAGTGAAGA AGAATGGCAT AGTTGACCGT 
TTATTTGAAC AGCAACAAAC AGATATGAAA AAAAAATTGT GGAAGTTTGT AGATGAACAA 
CTTGATTATT TGTTTGAAAA GCTTAAAAAA ATCCTACTAA AGTTTTGTGA TTCTGTAAAC 
TTTGAAAATG AGAATAGTGA AGGAAAACTT GGAAAAAAAT ATAAAGAGAG AACCCAACAT 
TCAAATTGTC AGAAGAAAAA AATGGACAAC AAAGAAATAC GGAGAGAAAA AGTGCTAAAA 
TCAGAAAATA CTGTGAATTT TAAATCTTCA CTGGGATGTG AAAAATCTGA AGAAAAACAT 
CAGGACCAAA ATAAAACCAA TGCTAGTATA GTAAAGCATG ATGTAAAAAG AACTTTTAGC 
ACTTGCAGTG ATAATACAAA GAACGCTGAA TGTAAAGAGC AGTTTCTGGA AAAGAGCTGC 
CCGAGTACCC CTAGGCCAGG AAAAGATGAA GGACATACTG AGGAGGAGGC ACAGGCGGCA 
CAGCATGCAA GTGCTAAGTC CGAACGGAGC TTTGAGATCC TTACTGAGCA GCAGGCATCC 
AGCCTTACTT TTAACTTAGT GAGTGATGCA CAGATGGGTG AGATATTTAA AAGCTTGCTA 
CAAGGTTCTG ATCTGTTGGA CACAAGTGGC ACTGAAAAGG CAGAGTGGGA ATTAAAGACT 
CCAGAGAAAC AGCTGCTGGA AAGCCTCAAG TGTGAATCTG CACCAGCTTG TGCAACAGAA 
GAGCTGGTTT CAGAGGGGGC TTCTCTTTGT CCAAAAGTGA TCAGTGATGA TAATTGGTCT 
TTATTATCAT CTGAAAAGGG TCCATCTTTA TCTTCAGGGC TTTCACTGCC AGTTCATCCT 
GATGTGTTAG ATGAAAATTG TATGTTTGAA GTATCTTCTA ACACTGCTTT AGGTAAAGAT 
AATGTATACA GCTCAGAAAA GAGTAAGCCC TGCATCTCTT CCATACTCTT AGAAGATCTT 
GCGGTCTCTT TAACAGTACC GTCACCTCTG AAATCAGATG GCCATTTGAG TTTCTTAAAG 
CCAGAAGTTT TGTCAACTTC AACTCCTGAA GAAGTTATTA GTGCACATTT TAGTGAGGAT 
GCTTTGCTTG AGGAAGAGGA TGCATCTGAA CAGGACATTC ATCTAGCTCT GGAGTCTGAT 
AACTCAAGCA GTAAGTCAAG CTGTTCATCA TGGACAAGCC GGTCTGTTGC TTCAGGCTTT 
CAGTACCACC CTAATCTTCC CATGCATGCT GTCATAATGG AAAAGTCCAA TGATCATTTC 
ATTGTGAAAA TACGGCGTGC AACACCATCT ACCTCCCCTG GCCTTAAACA TGGTGTGGTA 
GCTGAGGAGT CATTGACATC TTTGCCTAGA ACTGGAAAAG AAGCTGGTGT AGCAACAGAG 
AAAGAACCTA ACCTGTTTCA GAGTACAGTT TTAAAACCTG TCAAGGACTT GGAAAATACT 
GATAAAAATA TTGATAAGAG TAAACTAACT CATGAAGAAC AGAACTCTAT AGTACAAACA 
CAGGTTCCAG ATATATATGA ATTTCTTAAA GATGCCTCAA ATAAGGTGGT TCATTGTGAT 
CAAGTGGTTG ATGATTGCTT CAAGTTGCAT CAAGTATGGG AACCAAAAGT TTCTGAGAAC 
CTTCAAGAGT TGCCTTCAAT GGAGAAAATC CCACACTCTC TTGATAATCA TCTTCCTGAT 
ACACACATAG ATCTAACGAA AGATTCAGCC ACTGAGACCA AAAGCTTGGG GGAGCTAATG 
GAAGTAACAG TTTTAAATGT TGATCACTTG GAATGTTCTC AAACCAACTT AGATCAAGAT 
GCAGAGATAA CATGTAGTTC TTTACAGCCT GATACTATAG ATGCTTTTAT TGATTTGACA 
CATGATGCTT CAAGTGAGAG TAAAAATGAA GGTAGTGAAC CTGTGTTAGC TGTTGAAGGT 
ATGGGATGCC AGGTAATATG TATAGATGAG GATACCAACA AAGAAGGAAA GATGGGAAGG 
GCAAACAGTC CTTTAGAAAG TATTGTTGAA GAAACTTGTA TTGATTTGAC CTCAGAGTCT 
CCTGGCTCCT GTGAAATCAA GAGACATAAT TTAAAGTCGG AGCCTCCATC AAAGTTGGAT 
TGTTTAGAGT TGCCTGAAAC TCTGGGTAAT GGTCACAAGA AGAGGAAAAA CAGTCCTGGT 
GTTAGTCACT CTTCTCAGAA AAAACAAAGA AAGGACATAG ACTTAAGTAG TGAAAAGACC 
CAGAGACTTA GTCCCAATTC TGATAGAAAT GGTGATGCTC ACAGAAAGCA AGCGAGCAAG 
AAAAGAGAAC CTGCAGTAAA TGAAACGTCC TTGTCATCAG AGGCCAGCCC AGAGGTGAAG 
GGTTCAACAG CAGTACTTGC TGCTTCCCCA GCAAGCCTTT CTGCAAAAAA TGTTATCAAA 
AAGAAGGGAG AAATTATAGT TTCATGGACA AGAAATGATG ACCGGGAAAT TTTACTGGAA 
TGTCAGAAAA GAATGCCGTC CCTGAAAACA TTTACTTATT TAGCTGTCAA GCTGAATAAA 
AATCCAAATC AGGTTTCAGA GAGGTTCCAG CAGCTGAAAA AGCTCTTTGA GAAGTCAAAA 
TGCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036127.2
CDS	299..6187
Translation	MAADDDNGDGTGLFDVCPASPLKNNDEGSLDIYAGLDSAVSDSTARSCVSFRNCLDLYEEILTEEGTAKEATYNDLQIEYGKCQQQMKDLMKRFKEIQTQNLNLKNENQSLKKNISALIKTARVEINRKDEEINHLHQRLSEFPHFRNNHKTARTKDSQSTSPHLDDCSKTDHGVKSDVQKDVHPNTAQPNLEKEGKSHSEAQNPLHLSTGVEKHCANNVWSRSPYQVGEGNSNEDNRRGRSGTRHSQCSRGTDRTQKDLHSSCNDSEPRDKEANSRLQGHPEKHGNSEARTESKISESKSSTGMGYKSERSASSWEKETSRERPHTRVESQHDKNLEKQNERLQNMHRKELPSQDKTERKVDVKFKPAGEEQGHRGRVDRALPPHPKNDVKHYGFNKYHPEERRGREDCKRDRGMNSHGFQDRRCSSFLSSNRNSKYPHSKEVSVAHQWENTPFKAERHRTEDRRKRERENKEESRHVKSDKKSPPEHLQRTHKDTKKSTADGKRQTEPKHGKGAVSNSELSKGTDSKEGATKVESGPNEAKGKDLKLSFMEKLNLTLSPAKKQPACQDNPHQITGVPEPSGTCDSRSLETTGTVACLPSGSEHNREETKSELPEPKEALLATSQLRISIPENKMKEEKRLLFKSVENTVPCELLACGTEISLPAPVEIEQARCLLGSVEVEETCGGARTAASVVMHVLPEHASEDASQELDTKRHDGINACAISEGVKTKVILSPKAAAASESHLAPLVEEPSISLVNCSGDNNPKLEPSLEERPIVETKSCPLESCLPKETFVPSPQKTELIDHKIETGESNSVYQDDDNSVLSIDFNNLRPIPDPISPLNSPVRPVCKVVSMESSCAIPLYDSSHKDEFPSNSTLSTFKSQSDLNKENEKPVPKFDKCSEADSCKHLSLDELEEGEIRSDDEESVAQKRLEKSARPRVSAEVQPGKSSPGSRRSTVHVHKDNGRTAVKLPRDRLTWSKRSSESRPSNTERKSKTMSISSLEKILPLILVPSSLWEVMHMLRLLGKHVRKNYMKFKIKFSLTQFHRIIESAILSFTSLVKCLDLSKICKSVSTLQKSLCEVIESNLKQVKKNGIVDRLFEQQQTDMKKKLWKFVDEQLDYLFEKLKKILLKFCDSVNFENENSEGKLGKKYKERTQHSNCQKKKMDNKEIRREKVLKSENTVNFKSSLGCEKSEEKHQDQNKTNASIVKHDVKRTFSTCSDNTKNAECKEQFLEKSCPSTPRPGKDEGHTEEEAQAAQHASAKSERSFEILTEQQASSLTFNLVSDAQMGEIFKSLLQGSDLLDTSGTEKAEWELKTPEKQLLESLKCESAPACATEELVSEGASLCPKVISDDNWSLLSSEKGPSLSSGLSLPVHPDVLDENCMFEVSSNTALGKDNVYSSEKSKPCISSILLEDLAVSLTVPSPLKSDGHLSFLKPEVLSTSTPEEVISAHFSEDALLEEEDASEQDIHLALESDNSSSKSSCSSWTSRSVASGFQYHPNLPMHAVIMEKSNDHFIVKIRRATPSTSPGLKHGVVAEESLTSLPRTGKEAGVATEKEPNLFQSTVLKPVKDLENTDKNIDKSKLTHEEQNSIVQTQVPDIYEFLKDASNKVVHCDQVVDDCFKLHQVWEPKVSENLQELPSMEKIPHSLDNHLPDTHIDLTKDSATETKSLGELMEVTVLNVDHLECSQTNLDQDAEITCSSLQPDTIDAFIDLTHDASSESKNEGSEPVLAVEGMGCQVICIDEDTNKEGKMGRANSPLESIVEETCIDLTSESPGSCEIKRHNLKSEPPSKLDCLELPETLGNGHKKRKNSPGVSHSSQKKQRKDIDLSSEKTQRLSPNSDRNGDAHRKQASKKREPAVNETSLSSEASPEVKGSTAVLAASPASLSAKNVIKKKGEIIVSWTRNDDREILLECQKRMPSLKTFTYLAVKLNKNPNQVSERFQQLKKLFEKSKCR

Target ORF information:

RefSeq Version	NM_011997.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus caspase 8 associated protein 2 (Casp8ap2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011997.3

ORF Insert Sequence:

ATGGCAGCAG ATGATGACAA TGGTGATGGA ACGGGTTTGT TTGATGTCTG TCCTGCCTCT 
CCACTTAAAA ATAATGATGA AGGCTCATTG GACATATATG CTGGGTTGGA CAGTGCTGTT 
TCTGACAGTA CTGCTAGATC CTGTGTGTCA TTCAGAAACT GTTTAGATTT GTATGAAGAG 
ATCCTGACTG AAGAAGGAAC TGCGAAGGAG GCAACGTACA ATGACTTGCA GATAGAATAT 
GGGAAATGTC AGCAGCAAAT GAAAGACCTG ATGAAAAGGT TTAAGGAAAT ACAGACACAG 
AACTTGAACT TAAAAAATGA AAACCAGTCT CTTAAGAAGA ATATCTCAGC ACTTATCAAA 
ACTGCCAGAG TGGAGATAAA CCGTAAGGAT GAAGAGATAA ATCATCTTCA CCAAAGATTG 
TCTGAGTTTC CACATTTTCG AAATAACCAT AAAACTGCAA GAACAAAAGA TTCACAGTCC 
ACATCTCCCC ATTTGGATGA TTGTTCAAAG ACTGATCACG GAGTTAAAAG TGATGTTCAG 
AAAGATGTAC ATCCTAACAC TGCACAGCCA AACTTGGAAA AGGAAGGAAA ATCGCATTCT 
GAAGCACAGA ATCCTTTGCA CTTGTCTACG GGTGTTGAGA AACATTGTGC CAATAATGTC 
TGGTCACGTT CTCCTTACCA GGTTGGAGAA GGTAACTCAA ATGAGGATAA TAGGAGAGGA 
AGGAGTGGCA CTAGACATAG CCAGTGTAGC AGAGGAACTG ATAGAACACA GAAAGACTTA 
CATAGCAGCT GTAATGACAG TGAGCCAAGG GACAAGGAGG CTAACTCCAG ACTACAAGGA 
CACCCTGAGA AACATGGCAA CAGTGAAGCA AGGACGGAGA GCAAAATTTC AGAGAGTAAA 
AGCAGCACTG GTATGGGATA TAAAAGTGAG CGCAGTGCCT CTTCTTGGGA AAAAGAGACT 
TCCAGAGAAA GGCCACACAC TCGAGTGGAA TCTCAACATG ACAAAAATCT AGAGAAACAA 
AATGAAAGAT TACAAAATAT GCACAGAAAA GAGCTTCCGT CTCAGGACAA AACAGAAAGA 
AAAGTTGATG TGAAGTTTAA ACCAGCAGGA GAGGAGCAGG GGCATCGGGG AAGAGTGGAC 
CGGGCGTTAC CTCCTCATCC CAAGAATGAC GTGAAACATT ACGGCTTCAA TAAGTATCAT 
CCAGAAGAGA GAAGGGGAAG GGAAGATTGT AAAAGAGACA GAGGGATGAA CAGTCATGGC 
TTTCAAGATA GAAGATGTTC ATCTTTTCTT TCAAGCAACA GAAATAGCAA ATACCCGCAC 
TCCAAGGAAG TTAGTGTTGC ACACCAGTGG GAAAATACTC CTTTCAAAGC AGAAAGACAT 
AGAACTGAGG ACAGGAGGAA AAGAGAACGA GAAAACAAAG AAGAAAGTAG ACATGTGAAA 
AGTGACAAAA AATCACCTCC AGAACACTTA CAAAGGACTC ATAAAGACAC TAAGAAAAGC 
ACTGCTGATG GAAAGAGACA GACTGAGCCC AAACATGGTA AAGGTGCAGT CTCTAACAGT 
GAGCTTTCTA AAGGGACAGA CAGTAAAGAA GGTGCAACAA AGGTGGAGAG TGGGCCAAAT 
GAAGCAAAAG GCAAGGACTT AAAGTTAAGC TTCATGGAAA AACTGAACTT AACTCTTTCT 
CCTGCTAAAA AGCAGCCTGC TTGTCAAGAT AACCCACATC AAATAACTGG TGTTCCCGAG 
CCCAGTGGCA CGTGTGACTC ACGGTCCTTG GAGACGACTG GAACGGTGGC ATGCCTTCCT 
TCTGGCAGTG AACATAATAG AGAGGAAACC AAATCAGAGT TACCAGAGCC AAAGGAGGCT 
CTTTTGGCAA CATCTCAACT CAGGATCAGC ATTCCAGAAA ACAAAATGAA GGAAGAAAAG 
AGGTTGTTAT TTAAATCTGT TGAGAATACT GTGCCTTGTG AACTGCTTGC TTGTGGCACG 
GAAATTTCCC TCCCAGCACC TGTAGAAATT GAACAAGCAA GATGCTTGCT TGGATCAGTG 
GAAGTGGAAG AGACCTGTGG TGGTGCAAGG ACAGCCGCTT CTGTGGTGAT GCATGTGTTA 
CCAGAACATG CTTCTGAAGA TGCCAGCCAA GAATTGGACA CCAAAAGACA CGATGGTATA 
AATGCTTGTG CTATTTCTGA AGGTGTGAAA ACAAAGGTGA TTCTTTCACC AAAAGCAGCT 
GCAGCCAGTG AGAGCCATCT TGCACCTTTG GTTGAAGAAC CTAGCATTTC ACTAGTAAAC 
TGTTCGGGAG ACAATAATCC TAAACTTGAA CCTTCTCTTG AAGAGAGGCC TATAGTTGAG 
ACTAAATCCT GTCCTTTGGA GTCTTGTTTA CCTAAAGAGA CTTTTGTACC TTCACCACAG 
AAGACTGAGT TGATTGACCA CAAAATAGAA ACTGGAGAAT CAAACTCAGT ATATCAAGAT 
GATGATAACT CAGTTTTGAG TATTGACTTT AATAATCTGA GACCTATTCC AGACCCCATC 
AGCCCTCTGA ATAGTCCAGT GAGACCGGTA TGCAAAGTTG TTAGCATGGA GAGCTCATGT 
GCAATTCCAC TGTATGATAG CAGTCATAAA GATGAGTTTC CATCCAATTC AACTCTTTCT 
ACCTTCAAGA GTCAGTCTGA TCTAAATAAG GAAAATGAAA AACCAGTTCC CAAATTTGAC 
AAATGTTCAG AAGCAGATTC TTGTAAGCAT TTGTCTTTAG ATGAATTAGA AGAAGGCGAA 
ATTAGAAGTG ATGATGAAGA ATCTGTAGCA CAAAAACGCT TGGAAAAGAG TGCAAGACCT 
AGAGTTTCTG CTGAAGTGCA GCCAGGGAAA AGCAGCCCAG GAAGCAGGAG GAGCACTGTG 
CATGTGCATA AGGACAACGG GAGGACTGCT GTAAAACTCC CTCGGGACAG ACTCACATGG 
AGCAAAAGAT CAAGTGAATC AAGACCCTCC AACACCGAAA GGAAAAGTAA AACAATGAGC 
ATCTCCAGCT TGGAAAAAAT ACTTCCGCTT ATCCTTGTAC CCTCTTCCCT GTGGGAGGTT 
ATGCATATGT TACGGTTGCT AGGTAAACAT GTAAGAAAAA ATTATATGAA ATTCAAGATA 
AAATTTTCAT TGACACAATT TCATAGAATT ATTGAATCTG CAATTTTGAG TTTCACATCA 
CTAGTTAAAT GCCTTGATTT GTCTAAGATC TGTAAGTCAG TAAGTACTTT ACAAAAGAGT 
CTTTGTGAAG TTATAGAATC TAACCTTAAA CAAGTGAAGA AGAATGGCAT AGTTGACCGT 
TTATTTGAAC AGCAACAAAC AGATATGAAA AAAAAATTGT GGAAGTTTGT AGATGAACAA 
CTTGATTATT TGTTTGAAAA GCTTAAAAAA ATCCTACTAA AGTTTTGTGA TTCTGTAAAC 
TTTGAAAATG AGAATAGTGA AGGAAAACTT GGAAAAAAAT ATAAAGAGAG AACCCAACAT 
TCAAATTGTC AGAAGAAAAA AATGGACAAC AAAGAAATAC GGAGAGAAAA AGTGCTAAAA 
TCAGAAAATA CTGTGAATTT TAAATCTTCA CTGGGATGTG AAAAATCTGA AGAAAAACAT 
CAGGACCAAA ATAAAACCAA TGCTAGTATA GTAAAGCATG ATGTAAAAAG AACTTTTAGC 
ACTTGCAGTG ATAATACAAA GAACGCTGAA TGTAAAGAGC AGTTTCTGGA AAAGAGCTGC 
CCGAGTACCC CTAGGCCAGG AAAAGATGAA GGACATACTG AGGAGGAGGC ACAGGCGGCA 
CAGCATGCAA GTGCTAAGTC CGAACGGAGC TTTGAGATCC TTACTGAGCA GCAGGCATCC 
AGCCTTACTT TTAACTTAGT GAGTGATGCA CAGATGGGTG AGATATTTAA AAGCTTGCTA 
CAAGGTTCTG ATCTGTTGGA CACAAGTGGC ACTGAAAAGG CAGAGTGGGA ATTAAAGACT 
CCAGAGAAAC AGCTGCTGGA AAGCCTCAAG TGTGAATCTG CACCAGCTTG TGCAACAGAA 
GAGCTGGTTT CAGAGGGGGC TTCTCTTTGT CCAAAAGTGA TCAGTGATGA TAATTGGTCT 
TTATTATCAT CTGAAAAGGG TCCATCTTTA TCTTCAGGGC TTTCACTGCC AGTTCATCCT 
GATGTGTTAG ATGAAAATTG TATGTTTGAA GTATCTTCTA ACACTGCTTT AGGTAAAGAT 
AATGTATACA GCTCAGAAAA GAGTAAGCCC TGCATCTCTT CCATACTCTT AGAAGATCTT 
GCGGTCTCTT TAACAGTACC GTCACCTCTG AAATCAGATG GCCATTTGAG TTTCTTAAAG 
CCAGAAGTTT TGTCAACTTC AACTCCTGAA GAAGTTATTA GTGCACATTT TAGTGAGGAT 
GCTTTGCTTG AGGAAGAGGA TGCATCTGAA CAGGACATTC ATCTAGCTCT GGAGTCTGAT 
AACTCAAGCA GTAAGTCAAG CTGTTCATCA TGGACAAGCC GGTCTGTTGC TTCAGGCTTT 
CAGTACCACC CTAATCTTCC CATGCATGCT GTCATAATGG AAAAGTCCAA TGATCATTTC 
ATTGTGAAAA TACGGCGTGC AACACCATCT ACCTCCCCTG GCCTTAAACA TGGTGTGGTA 
GCTGAGGAGT CATTGACATC TTTGCCTAGA ACTGGAAAAG AAGCTGGTGT AGCAACAGAG 
AAAGAACCTA ACCTGTTTCA GAGTACAGTT TTAAAACCTG TCAAGGACTT GGAAAATACT 
GATAAAAATA TTGATAAGAG TAAACTAACT CATGAAGAAC AGAACTCTAT AGTACAAACA 
CAGGTTCCAG ATATATATGA ATTTCTTAAA GATGCCTCAA ATAAGGTGGT TCATTGTGAT 
CAAGTGGTTG ATGATTGCTT CAAGTTGCAT CAAGTATGGG AACCAAAAGT TTCTGAGAAC 
CTTCAAGAGT TGCCTTCAAT GGAGAAAATC CCACACTCTC TTGATAATCA TCTTCCTGAT 
ACACACATAG ATCTAACGAA AGATTCAGCC ACTGAGACCA AAAGCTTGGG GGAGCTAATG 
GAAGTAACAG TTTTAAATGT TGATCACTTG GAATGTTCTC AAACCAACTT AGATCAAGAT 
GCAGAGATAA CATGTAGTTC TTTACAGCCT GATACTATAG ATGCTTTTAT TGATTTGACA 
CATGATGCTT CAAGTGAGAG TAAAAATGAA GGTAGTGAAC CTGTGTTAGC TGTTGAAGGT 
ATGGGATGCC AGGTAATATG TATAGATGAG GATACCAACA AAGAAGGAAA GATGGGAAGG 
GCAAACAGTC CTTTAGAAAG TATTGTTGAA GAAACTTGTA TTGATTTGAC CTCAGAGTCT 
CCTGGCTCCT GTGAAATCAA GAGACATAAT TTAAAGTCGG AGCCTCCATC AAAGTTGGAT 
TGTTTAGAGT TGCCTGAAAC TCTGGGTAAT GGTCACAAGA AGAGGAAAAA CAGTCCTGGT 
GTTAGTCACT CTTCTCAGAA AAAACAAAGA AAGGACATAG ACTTAAGTAG TGAAAAGACC 
CAGAGACTTA GTCCCAATTC TGATAGAAAT GGTGATGCTC ACAGAAAGCA AGCGAGCAAG 
AAAAGAGAAC CTGCAGTAAA TGAAACGTCC TTGTCATCAG AGGCCAGCCC AGAGGTGAAG 
GGTTCAACAG CAGTACTTGC TGCTTCCCCA GCAAGCCTTT CTGCAAAAAA TGTTATCAAA 
AAGAAGGGAG AAATTATAGT TTCATGGACA AGAAATGATG ACCGGGAAAT TTTACTGGAA 
TGTCAGAAAA GAATGCCGTC CCTGAAAACA TTTACTTATT TAGCTGTCAA GCTGAATAAA 
AATCCAAATC AGGTTTCAGA GAGGTTCCAG CAGCTGAAAA AGCTCTTTGA GAAGTCAAAA 
TGCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Casp8ap2 ( XM_006537950.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006538013.1 Mus musculus caspase 8 associated protein 2 (Casp8ap2), transcript variant X2, mRNA.

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CloneID	OMu32946
Clone ID Related Accession (Same CDS sequence)	XM_006537950.1 , XM_006537950.2
Accession Version	XM_006537950.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6015bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	CASP8-associated protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006537950.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTACG CTCTGGGCGA AGGGTTGTGC CTGGCTGGGC CTCGAGGAGC CAGCTGCCGT 
CCTCGGGGAG CGCCCCTCGG AGCGTCTCGG GGGGCGCTGG GGCGAGCACC CGGAACATCG 
CCGGGTCTAC AGCGCGCCCC TCCCCTCCCC CACCCGTGTC TCCCAAGTCC CTACTGCCGA 
GCTCTCGCCT CTCCACTTAA AAATAATGAT GAAGGCTCAT TGGACATATA TGCTGGGTTG 
GACAGTGCTG TTTCTGACAG TACTGCTAGA TCCTGTGTGT CATTCAGAAA CTGTTTAGAT 
TTGTATGAAG AGATCCTGAC TGAAGAAGGA ACTGCGAAGG AGGCAACGTA CAATGACATA 
GAATATGGGA AATGTCAGCA GCAAATGAAA GACCTGATGA AAAGGTTTAA GGAAATACAG 
ACACAGAACT TGAACTTAAA AAATGAAAAC CAGTCTCTTA AGAAGAATAT CTCAGCACTT 
ATCAAAACTG CCAGAGTGGA GATAAACCGT AAGGATGAAG AGATAAATCA TCTTCACCAA 
AGATTGTCTG AGTTTCCACA TTTTCGAAAT AACCATAAAA CTGCAAGAAC AAAAGATTCA 
CAGTCCACAT CTCCCCATTT GGATGATTGT TCAAAGACTG ATCACGGAGT TAAAAGTGAT 
GTTCAGAAAG ATGTACATCC TAACACTGCA CAGCCAAACT TGGAAAAGGA AGGAAAATCG 
CATTCTGAAG CACAGAATCC TTTGCACTTG TCTACGGGTG TTGAGAAACA TTGTGCCAAT 
AATGTCTGGT CACGTTCTCC TTACCAGGTT GGAGAAGGTA ACTCAAATGA GGATAATAGG 
AGAGGAAGGA GTGGCACTAG ACATAGCCAG TGTAGCAGAG GAACTGATAG AACACAGAAA 
GACTTACATA GCAGCTGTAA TGACAGTGAG CCAAGGGACA AGGAGGCTAA CTCCAGACTA 
CAAGGACACC CTGAGAAACA TGGCAACAGT GAAGCAAGGA CGGAGAGCAA AATTTCAGAG 
AGTAAAAGCA GCACTGGTAT GGGATATAAA AGTGAGCGCA GTGCCTCTTC TTGGGAAAAA 
GAGACTTCCA GAGAAAGGCC ACACACTCGA GTGGAATCTC AACATGACAA AAATCTAGAG 
AAACAAAATG AAAGATTACA AAATATGCAC AGAAAAGAGC TTCCGTCTCA GGACAAAACA 
GAAAGAAAAG TTGATGTGAA GTTTAAACCA GCAGGAGAGG AGCAGGGGCA TCGGGGAAGA 
GTGGACCGGG CGTTACCTCC TCATCCCAAG AATGACGTGA AACATTACGG CTTCAATAAG 
TATCATCCAG AAGAGAGAAG GGGAAGGGAA GATTGTAAAA GAGACAGAGG GATGAACAGT 
CATGGCTTTC AAGATAGAAG ATGTTCATCT TTTCTTTCAA GCAACAGAAA TAGCAAATAC 
CCGCACTCCA AGGAAGTTAG TGTTGCACAC CAGTGGGAAA ATACTCCTTT CAAAGCAGAA 
AGACATAGAA CTGAGGACAG GAGGAAAAGA GAACGAGAAA ACAAAGAAGA AAGTAGACAT 
GTGAAAAGTG ACAAAAAATC ACCTCCAGAA CACTTACAAA GGACTCATAA AGACACTAAG 
AAAAGCACTG CTGATGGAAA GAGACAGACT GAGCCCAAAC ATGGTAAAGG TGCAGTCTCT 
AACAGTGAGC TTTCTAAAGG GACAGACAGT AAAGAAGGTG CAACAAAGGT GGAGAGTGGG 
CCAAATGAAG CAAAAGGCAA GGACTTAAAG TTAAGCTTCA TGGAAAAACT GAACTTAACT 
CTTTCTCCTG CTAAAAAGCA GCCTGCTTGT CAAGATAACC CACATCAAAT AACTGGTGTT 
CCCGAGCCCA GTGGCACGTG TGACTCACGG TCCTTGGAGA CGACTGGAAC GGTGGCATGC 
CTTCCTTCTG GCAGTGAACA TAATAGAGAG GAAACCAAAT CAGAGTTACC AGAGCCAAAG 
GAGGCTCTTT TGGCAACATC TCAACTCAGG ATCAGCATTC CAGAAAACAA AATGAAGGAA 
GAAAAGAGGT TGTTATTTAA ATCTGTTGAG AATACTGTGC CTTGTGAACT GCTTGCTTGT 
GGCACGGAAA TTTCCCTCCC AGCACCTGTA GAAATTGAAC AAGCAAGATG CTTGCTTGGA 
TCAGTGGAAG TGGAAGAGAC CTGTGGTGGT GCAAGGACAG CCGCTTCTGT GGTGATGCAT 
GTGTTACCAG AACATGCTTC TGAAGATGCC AGCCAAGAAT TGGACACCAA AAGACACGAT 
GGTATAAATG CTTGTGCTAT TTCTGAAGGT GTGAAAACAA AGGTGATTCT TTCACCAAAA 
GCAGCTGCAG CCAGTGAGAG CCATCTTGCA CCTTTGGTTG AAGAACCTAG CATTTCACTA 
GTAAACTGTT CGGGAGACAA TAATCCTAAA CTTGAACCTT CTCTTGAAGA GAGGCCTATA 
GTTGAGACTA AATCCTGTCC TTTGGAGTCT TGTTTACCTA AAGAGACTTT TGTACCTTCA 
CCACAGAAGA CTGAGTTGAT TGACCACAAA ATAGAAACTG GAGAATCAAA CTCAGTATAT 
CAAGATGATG ATAACTCAGT TTTGAGTATT GACTTTAATA ATCTGAGACC TATTCCAGAC 
CCCATCAGCC CTCTGAATAG TCCAGTGAGA CCGGTATGCA AAGTTGTTAG CATGGAGAGC 
TCATGTGCAA TTCCACTGTA TGATAGCAGT CATAAAGATG AGTTTCCATC CAATTCAACT 
CTTTCTACCT TCAAGAGTCA GTCTGATCTA AATAAGGAAA ATGAAAAACC AGTTCCCAAA 
TTTGACAAAT GTTCAGAAGC AGATTCTTGT AAGCATTTGT CTTTAGATGA ATTAGAAGAA 
GGCGAAATTA GAAGTGATGA TGAAGAATCT GTAGCACAAA AACGCTTGGA AAAGAGTGCA 
AGACCTAGAG TTTCTGCTGA AGTGCAGCCA GGGAAAAGCA GCCCAGGAAG CAGGAGGAGC 
ACTGTGCATG TGCATAAGGA CAACGGGAGG ACTGCTGTAA AACTCCCTCG GGACAGACTC 
ACATGGAGCA AAAGATCAAG TGAATCAAGA CCCTCCAACA CCGAAAGGAA AAGTAAAACA 
ATGAGCATCT CCAGCTTGGA AAAAATACTT CCGCTTATCC TTGTACCCTC TTCCCTGTGG 
GAGGTTATGC ATATGTTACG GTTGCTAGGT AAACATGTAA GAAAAAATTA TATGAAATTC 
AAGATAAAAT TTTCATTGAC ACAATTTCAT AGAATTATTG AATCTGCAAT TTTGAGTTTC 
ACATCACTAG TTAAATGCCT TGATTTGTCT AAGATCTGTA AGTCAGTAAG TACTTTACAA 
AAGAGTCTTT GTGAAGTTAT AGAATCTAAC CTTAAACAAG TGAAGAAGAA TGGCATAGTT 
GACCGTTTAT TTGAACAGCA ACAAACAGAT ATGAAAAAAA AATTGTGGAA GTTTGTAGAT 
GAACAACTTG ATTATTTGTT TGAAAAGCTT AAAAAAATCC TACTAAAGTT TTGTGATTCT 
GTAAACTTTG AAAATGAGAA TAGTGAAGGA AAACTTGGAA AAAAATATAA AGAGAGAACC 
CAACATTCAA ATTGTCAGAA GAAAAAAATG GACAACAAAG AAATACGGAG AGAAAAAGTG 
CTAAAATCAG AAAATACTGT GAATTTTAAA TCTTCACTGG GATGTGAAAA ATCTGAAGAA 
AAACATCAGG ACCAAAATAA AACCAATGCT AGTATAGTAA AGCATGATGT AAAAAGAACT 
TTTAGCACTT GCAGTGATAA TACAAAGAAC GCTGAATGTA AAGAGCAGTT TCTGGAAAAG 
AGCTGCCCGA GTACCCCTAG GCCAGGAAAA GATGAAGGAC ATACTGAGGA GGAGGCACAG 
GCGGCACAGC ATGCAAGTGC TAAGTCCGAA CGGAGCTTTG AGATCCTTAC TGAGCAGCAG 
GCATCCAGCC TTACTTTTAA CTTAGTGAGT GATGCACAGA TGGGTGAGAT ATTTAAAAGC 
TTGCTACAAG GTTCTGATCT GTTGGACACA AGTGGCACTG AAAAGGCAGA GTGGGAATTA 
AAGACTCCAG AGAAACAGCT GCTGGAAAGC CTCAAGTGTG AATCTGCACC AGCTTGTGCA 
ACAGAAGAGC TGGTTTCAGA GGGGGCTTCT CTTTGTCCAA AAGTGATCAG TGATGATAAT 
TGGTCTTTAT TATCATCTGA AAAGGGTCCA TCTTTATCTT CAGGGCTTTC ACTGCCAGTT 
CATCCTGATG TGTTAGATGA AAATTGTATG TTTGAAGTAT CTTCTAACAC TGCTTTAGGT 
AAAGATAATG TATACAGCTC AGAAAAGAGT AAGCCCTGCA TCTCTTCCAT ACTCTTAGAA 
GATCTTGCGG TCTCTTTAAC AGTACCGTCA CCTCTGAAAT CAGATGGCCA TTTGAGTTTC 
TTAAAGCCAG AAGTTTTGTC AACTTCAACT CCTGAAGAAG TTATTAGTGC ACATTTTAGT 
GAGGATGCTT TGCTTGAGGA AGAGGATGCA TCTGAACAGG ACATTCATCT AGCTCTGGAG 
TCTGATAACT CAAGCAGTAA GTCAAGCTGT TCATCATGGA CAAGCCGGTC TGTTGCTTCA 
GGCTTTCAGT ACCACCCTAA TCTTCCCATG CATGCTGTCA TAATGGAAAA GTCCAATGAT 
CATTTCATTG TGAAAATACG GCGTGCAACA CCATCTACCT CCCCTGGCCT TAAACATGGT 
GTGGTAGCTG AGGAGTCATT GACATCTTTG CCTAGAACTG GAAAAGAAGC TGGTGTAGCA 
ACAGAGAAAG AACCTAACCT GTTTCAGAGT ACAGTTTTAA AACCTGTCAA GGACTTGGAA 
AATACTGATA AAAATATTGA TAAGAGTAAA CTAACTCATG AAGAACAGAA CTCTATAGTA 
CAAACACAGG TTCCAGATAT ATATGAATTT CTTAAAGATG CCTCAAATAA GGTGGTTCAT 
TGTGATCAAG TGGTTGATGA TTGCTTCAAG TTGCATCAAG TATGGGAACC AAAAGTTTCT 
GAGAACCTTC AAGAGTTGCC TTCAATGGAG AAAATCCCAC ACTCTCTTGA TAATCATCTT 
CCTGATACAC ACATAGATCT AACGAAAGAT TCAGCCACTG AGACCAAAAG CTTGGGGGAG 
CTAATGGAAG TAACAGTTTT AAATGTTGAT CACTTGGAAT GTTCTCAAAC CAACTTAGAT 
CAAGATGCAG AGATAACATG TAGTTCTTTA CAGCCTGATA CTATAGATGC TTTTATTGAT 
TTGACACATG ATGCTTCAAG TGAGAGTAAA AATGAAGGTA GTGAACCTGT GTTAGCTGTT 
GAAGGTATGG GATGCCAGGT AATATGTATA GATGAGGATA CCAACAAAGA AGGAAAGATG 
GGAAGGGCAA ACAGTCCTTT AGAAAGTATT GTTGAAGAAA CTTGTATTGA TTTGACCTCA 
GAGTCTCCTG GCTCCTGTGA AATCAAGAGA CATAATTTAA AGTCGGAGCC TCCATCAAAG 
TTGGATTGTT TAGAGTTGCC TGAAACTCTG GGTAATGGTC ACAAGAAGAG GAAAAACAGT 
CCTGGTGTTA GTCACTCTTC TCAGAAAAAA CAAAGAAAGG ACATAGACTT AAGTAGTGAA 
AAGACCCAGA GACTTAGTCC CAATTCTGAT AGAAATGGTG ATGCTCACAG AAAGCAAGCG 
AGCAAGAAAA GAGAACCTGC AGTAAATGAA ACGTCCTTGT CATCAGAGGC CAGCCCAGAG 
GTGAAGGGTT CAACAGCAGT ACTTGCTGCT TCCCCAGCAA GCCTTTCTGC AAAAAATGTT 
ATCAAAAAGA AGGGAGAAAT TATAGTTTCA TGGACAAGAA ATGATGACCG GGAAATTTTA 
CTGGAATGTC AGAAAAGAAT GCCGTCCCTG AAAACATTTA CTTATTTAGC TGTCAAGCTG 
AATAAAAATC CAAATCAGGT TTCAGAGAGG TTCCAGCAGC TGAAAAAGCT CTTTGAGAAG 
TCAAAATGCA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006538013.1
CDS	55..6069
Translation	MAYALGEGLCLAGPRGASCRPRGAPLGASRGALGRAPGTSPGLQRAPPLPHPCLPSPYCRALASPLKNNDEGSLDIYAGLDSAVSDSTARSCVSFRNCLDLYEEILTEEGTAKEATYNDIEYGKCQQQMKDLMKRFKEIQTQNLNLKNENQSLKKNISALIKTARVEINRKDEEINHLHQRLSEFPHFRNNHKTARTKDSQSTSPHLDDCSKTDHGVKSDVQKDVHPNTAQPNLEKEGKSHSEAQNPLHLSTGVEKHCANNVWSRSPYQVGEGNSNEDNRRGRSGTRHSQCSRGTDRTQKDLHSSCNDSEPRDKEANSRLQGHPEKHGNSEARTESKISESKSSTGMGYKSERSASSWEKETSRERPHTRVESQHDKNLEKQNERLQNMHRKELPSQDKTERKVDVKFKPAGEEQGHRGRVDRALPPHPKNDVKHYGFNKYHPEERRGREDCKRDRGMNSHGFQDRRCSSFLSSNRNSKYPHSKEVSVAHQWENTPFKAERHRTEDRRKRERENKEESRHVKSDKKSPPEHLQRTHKDTKKSTADGKRQTEPKHGKGAVSNSELSKGTDSKEGATKVESGPNEAKGKDLKLSFMEKLNLTLSPAKKQPACQDNPHQITGVPEPSGTCDSRSLETTGTVACLPSGSEHNREETKSELPEPKEALLATSQLRISIPENKMKEEKRLLFKSVENTVPCELLACGTEISLPAPVEIEQARCLLGSVEVEETCGGARTAASVVMHVLPEHASEDASQELDTKRHDGINACAISEGVKTKVILSPKAAAASESHLAPLVEEPSISLVNCSGDNNPKLEPSLEERPIVETKSCPLESCLPKETFVPSPQKTELIDHKIETGESNSVYQDDDNSVLSIDFNNLRPIPDPISPLNSPVRPVCKVVSMESSCAIPLYDSSHKDEFPSNSTLSTFKSQSDLNKENEKPVPKFDKCSEADSCKHLSLDELEEGEIRSDDEESVAQKRLEKSARPRVSAEVQPGKSSPGSRRSTVHVHKDNGRTAVKLPRDRLTWSKRSSESRPSNTERKSKTMSISSLEKILPLILVPSSLWEVMHMLRLLGKHVRKNYMKFKIKFSLTQFHRIIESAILSFTSLVKCLDLSKICKSVSTLQKSLCEVIESNLKQVKKNGIVDRLFEQQQTDMKKKLWKFVDEQLDYLFEKLKKILLKFCDSVNFENENSEGKLGKKYKERTQHSNCQKKKMDNKEIRREKVLKSENTVNFKSSLGCEKSEEKHQDQNKTNASIVKHDVKRTFSTCSDNTKNAECKEQFLEKSCPSTPRPGKDEGHTEEEAQAAQHASAKSERSFEILTEQQASSLTFNLVSDAQMGEIFKSLLQGSDLLDTSGTEKAEWELKTPEKQLLESLKCESAPACATEELVSEGASLCPKVISDDNWSLLSSEKGPSLSSGLSLPVHPDVLDENCMFEVSSNTALGKDNVYSSEKSKPCISSILLEDLAVSLTVPSPLKSDGHLSFLKPEVLSTSTPEEVISAHFSEDALLEEEDASEQDIHLALESDNSSSKSSCSSWTSRSVASGFQYHPNLPMHAVIMEKSNDHFIVKIRRATPSTSPGLKHGVVAEESLTSLPRTGKEAGVATEKEPNLFQSTVLKPVKDLENTDKNIDKSKLTHEEQNSIVQTQVPDIYEFLKDASNKVVHCDQVVDDCFKLHQVWEPKVSENLQELPSMEKIPHSLDNHLPDTHIDLTKDSATETKSLGELMEVTVLNVDHLECSQTNLDQDAEITCSSLQPDTIDAFIDLTHDASSESKNEGSEPVLAVEGMGCQVICIDEDTNKEGKMGRANSPLESIVEETCIDLTSESPGSCEIKRHNLKSEPPSKLDCLELPETLGNGHKKRKNSPGVSHSSQKKQRKDIDLSSEKTQRLSPNSDRNGDAHRKQASKKREPAVNETSLSSEASPEVKGSTAVLAASPASLSAKNVIKKKGEIIVSWTRNDDREILLECQKRMPSLKTFTYLAVKLNKNPNQVSERFQQLKKLFEKSKCR

Target ORF information:

RefSeq Version	XM_006537950.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus caspase 8 associated protein 2 (Casp8ap2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006537950.2

ORF Insert Sequence:

ATGGCTTACG CTCTGGGCGA AGGGTTGTGC CTGGCTGGGC CTCGAGGAGC CAGCTGCCGT 
CCTCGGGGAG CGCCCCTCGG AGCGTCTCGG GGGGCGCTGG GGCGAGCACC CGGAACATCG 
CCGGGTCTAC AGCGCGCCCC TCCCCTCCCC CACCCGTGTC TCCCAAGTCC CTACTGCCGA 
GCTCTCGCCT CTCCACTTAA AAATAATGAT GAAGGCTCAT TGGACATATA TGCTGGGTTG 
GACAGTGCTG TTTCTGACAG TACTGCTAGA TCCTGTGTGT CATTCAGAAA CTGTTTAGAT 
TTGTATGAAG AGATCCTGAC TGAAGAAGGA ACTGCGAAGG AGGCAACGTA CAATGACATA 
GAATATGGGA AATGTCAGCA GCAAATGAAA GACCTGATGA AAAGGTTTAA GGAAATACAG 
ACACAGAACT TGAACTTAAA AAATGAAAAC CAGTCTCTTA AGAAGAATAT CTCAGCACTT 
ATCAAAACTG CCAGAGTGGA GATAAACCGT AAGGATGAAG AGATAAATCA TCTTCACCAA 
AGATTGTCTG AGTTTCCACA TTTTCGAAAT AACCATAAAA CTGCAAGAAC AAAAGATTCA 
CAGTCCACAT CTCCCCATTT GGATGATTGT TCAAAGACTG ATCACGGAGT TAAAAGTGAT 
GTTCAGAAAG ATGTACATCC TAACACTGCA CAGCCAAACT TGGAAAAGGA AGGAAAATCG 
CATTCTGAAG CACAGAATCC TTTGCACTTG TCTACGGGTG TTGAGAAACA TTGTGCCAAT 
AATGTCTGGT CACGTTCTCC TTACCAGGTT GGAGAAGGTA ACTCAAATGA GGATAATAGG 
AGAGGAAGGA GTGGCACTAG ACATAGCCAG TGTAGCAGAG GAACTGATAG AACACAGAAA 
GACTTACATA GCAGCTGTAA TGACAGTGAG CCAAGGGACA AGGAGGCTAA CTCCAGACTA 
CAAGGACACC CTGAGAAACA TGGCAACAGT GAAGCAAGGA CGGAGAGCAA AATTTCAGAG 
AGTAAAAGCA GCACTGGTAT GGGATATAAA AGTGAGCGCA GTGCCTCTTC TTGGGAAAAA 
GAGACTTCCA GAGAAAGGCC ACACACTCGA GTGGAATCTC AACATGACAA AAATCTAGAG 
AAACAAAATG AAAGATTACA AAATATGCAC AGAAAAGAGC TTCCGTCTCA GGACAAAACA 
GAAAGAAAAG TTGATGTGAA GTTTAAACCA GCAGGAGAGG AGCAGGGGCA TCGGGGAAGA 
GTGGACCGGG CGTTACCTCC TCATCCCAAG AATGACGTGA AACATTACGG CTTCAATAAG 
TATCATCCAG AAGAGAGAAG GGGAAGGGAA GATTGTAAAA GAGACAGAGG GATGAACAGT 
CATGGCTTTC AAGATAGAAG ATGTTCATCT TTTCTTTCAA GCAACAGAAA TAGCAAATAC 
CCGCACTCCA AGGAAGTTAG TGTTGCACAC CAGTGGGAAA ATACTCCTTT CAAAGCAGAA 
AGACATAGAA CTGAGGACAG GAGGAAAAGA GAACGAGAAA ACAAAGAAGA AAGTAGACAT 
GTGAAAAGTG ACAAAAAATC ACCTCCAGAA CACTTACAAA GGACTCATAA AGACACTAAG 
AAAAGCACTG CTGATGGAAA GAGACAGACT GAGCCCAAAC ATGGTAAAGG TGCAGTCTCT 
AACAGTGAGC TTTCTAAAGG GACAGACAGT AAAGAAGGTG CAACAAAGGT GGAGAGTGGG 
CCAAATGAAG CAAAAGGCAA GGACTTAAAG TTAAGCTTCA TGGAAAAACT GAACTTAACT 
CTTTCTCCTG CTAAAAAGCA GCCTGCTTGT CAAGATAACC CACATCAAAT AACTGGTGTT 
CCCGAGCCCA GTGGCACGTG TGACTCACGG TCCTTGGAGA CGACTGGAAC GGTGGCATGC 
CTTCCTTCTG GCAGTGAACA TAATAGAGAG GAAACCAAAT CAGAGTTACC AGAGCCAAAG 
GAGGCTCTTT TGGCAACATC TCAACTCAGG ATCAGCATTC CAGAAAACAA AATGAAGGAA 
GAAAAGAGGT TGTTATTTAA ATCTGTTGAG AATACTGTGC CTTGTGAACT GCTTGCTTGT 
GGCACGGAAA TTTCCCTCCC AGCACCTGTA GAAATTGAAC AAGCAAGATG CTTGCTTGGA 
TCAGTGGAAG TGGAAGAGAC CTGTGGTGGT GCAAGGACAG CCGCTTCTGT GGTGATGCAT 
GTGTTACCAG AACATGCTTC TGAAGATGCC AGCCAAGAAT TGGACACCAA AAGACACGAT 
GGTATAAATG CTTGTGCTAT TTCTGAAGGT GTGAAAACAA AGGTGATTCT TTCACCAAAA 
GCAGCTGCAG CCAGTGAGAG CCATCTTGCA CCTTTGGTTG AAGAACCTAG CATTTCACTA 
GTAAACTGTT CGGGAGACAA TAATCCTAAA CTTGAACCTT CTCTTGAAGA GAGGCCTATA 
GTTGAGACTA AATCCTGTCC TTTGGAGTCT TGTTTACCTA AAGAGACTTT TGTACCTTCA 
CCACAGAAGA CTGAGTTGAT TGACCACAAA ATAGAAACTG GAGAATCAAA CTCAGTATAT 
CAAGATGATG ATAACTCAGT TTTGAGTATT GACTTTAATA ATCTGAGACC TATTCCAGAC 
CCCATCAGCC CTCTGAATAG TCCAGTGAGA CCGGTATGCA AAGTTGTTAG CATGGAGAGC 
TCATGTGCAA TTCCACTGTA TGATAGCAGT CATAAAGATG AGTTTCCATC CAATTCAACT 
CTTTCTACCT TCAAGAGTCA GTCTGATCTA AATAAGGAAA ATGAAAAACC AGTTCCCAAA 
TTTGACAAAT GTTCAGAAGC AGATTCTTGT AAGCATTTGT CTTTAGATGA ATTAGAAGAA 
GGCGAAATTA GAAGTGATGA TGAAGAATCT GTAGCACAAA AACGCTTGGA AAAGAGTGCA 
AGACCTAGAG TTTCTGCTGA AGTGCAGCCA GGGAAAAGCA GCCCAGGAAG CAGGAGGAGC 
ACTGTGCATG TGCATAAGGA CAACGGGAGG ACTGCTGTAA AACTCCCTCG GGACAGACTC 
ACATGGAGCA AAAGATCAAG TGAATCAAGA CCCTCCAACA CCGAAAGGAA AAGTAAAACA 
ATGAGCATCT CCAGCTTGGA AAAAATACTT CCGCTTATCC TTGTACCCTC TTCCCTGTGG 
GAGGTTATGC ATATGTTACG GTTGCTAGGT AAACATGTAA GAAAAAATTA TATGAAATTC 
AAGATAAAAT TTTCATTGAC ACAATTTCAT AGAATTATTG AATCTGCAAT TTTGAGTTTC 
ACATCACTAG TTAAATGCCT TGATTTGTCT AAGATCTGTA AGTCAGTAAG TACTTTACAA 
AAGAGTCTTT GTGAAGTTAT AGAATCTAAC CTTAAACAAG TGAAGAAGAA TGGCATAGTT 
GACCGTTTAT TTGAACAGCA ACAAACAGAT ATGAAAAAAA AATTGTGGAA GTTTGTAGAT 
GAACAACTTG ATTATTTGTT TGAAAAGCTT AAAAAAATCC TACTAAAGTT TTGTGATTCT 
GTAAACTTTG AAAATGAGAA TAGTGAAGGA AAACTTGGAA AAAAATATAA AGAGAGAACC 
CAACATTCAA ATTGTCAGAA GAAAAAAATG GACAACAAAG AAATACGGAG AGAAAAAGTG 
CTAAAATCAG AAAATACTGT GAATTTTAAA TCTTCACTGG GATGTGAAAA ATCTGAAGAA 
AAACATCAGG ACCAAAATAA AACCAATGCT AGTATAGTAA AGCATGATGT AAAAAGAACT 
TTTAGCACTT GCAGTGATAA TACAAAGAAC GCTGAATGTA AAGAGCAGTT TCTGGAAAAG 
AGCTGCCCGA GTACCCCTAG GCCAGGAAAA GATGAAGGAC ATACTGAGGA GGAGGCACAG 
GCGGCACAGC ATGCAAGTGC TAAGTCCGAA CGGAGCTTTG AGATCCTTAC TGAGCAGCAG 
GCATCCAGCC TTACTTTTAA CTTAGTGAGT GATGCACAGA TGGGTGAGAT ATTTAAAAGC 
TTGCTACAAG GTTCTGATCT GTTGGACACA AGTGGCACTG AAAAGGCAGA GTGGGAATTA 
AAGACTCCAG AGAAACAGCT GCTGGAAAGC CTCAAGTGTG AATCTGCACC AGCTTGTGCA 
ACAGAAGAGC TGGTTTCAGA GGGGGCTTCT CTTTGTCCAA AAGTGATCAG TGATGATAAT 
TGGTCTTTAT TATCATCTGA AAAGGGTCCA TCTTTATCTT CAGGGCTTTC ACTGCCAGTT 
CATCCTGATG TGTTAGATGA AAATTGTATG TTTGAAGTAT CTTCTAACAC TGCTTTAGGT 
AAAGATAATG TATACAGCTC AGAAAAGAGT AAGCCCTGCA TCTCTTCCAT ACTCTTAGAA 
GATCTTGCGG TCTCTTTAAC AGTACCGTCA CCTCTGAAAT CAGATGGCCA TTTGAGTTTC 
TTAAAGCCAG AAGTTTTGTC AACTTCAACT CCTGAAGAAG TTATTAGTGC ACATTTTAGT 
GAGGATGCTT TGCTTGAGGA AGAGGATGCA TCTGAACAGG ACATTCATCT AGCTCTGGAG 
TCTGATAACT CAAGCAGTAA GTCAAGCTGT TCATCATGGA CAAGCCGGTC TGTTGCTTCA 
GGCTTTCAGT ACCACCCTAA TCTTCCCATG CATGCTGTCA TAATGGAAAA GTCCAATGAT 
CATTTCATTG TGAAAATACG GCGTGCAACA CCATCTACCT CCCCTGGCCT TAAACATGGT 
GTGGTAGCTG AGGAGTCATT GACATCTTTG CCTAGAACTG GAAAAGAAGC TGGTGTAGCA 
ACAGAGAAAG AACCTAACCT GTTTCAGAGT ACAGTTTTAA AACCTGTCAA GGACTTGGAA 
AATACTGATA AAAATATTGA TAAGAGTAAA CTAACTCATG AAGAACAGAA CTCTATAGTA 
CAAACACAGG TTCCAGATAT ATATGAATTT CTTAAAGATG CCTCAAATAA GGTGGTTCAT 
TGTGATCAAG TGGTTGATGA TTGCTTCAAG TTGCATCAAG TATGGGAACC AAAAGTTTCT 
GAGAACCTTC AAGAGTTGCC TTCAATGGAG AAAATCCCAC ACTCTCTTGA TAATCATCTT 
CCTGATACAC ACATAGATCT AACGAAAGAT TCAGCCACTG AGACCAAAAG CTTGGGGGAG 
CTAATGGAAG TAACAGTTTT AAATGTTGAT CACTTGGAAT GTTCTCAAAC CAACTTAGAT 
CAAGATGCAG AGATAACATG TAGTTCTTTA CAGCCTGATA CTATAGATGC TTTTATTGAT 
TTGACACATG ATGCTTCAAG TGAGAGTAAA AATGAAGGTA GTGAACCTGT GTTAGCTGTT 
GAAGGTATGG GATGCCAGGT AATATGTATA GATGAGGATA CCAACAAAGA AGGAAAGATG 
GGAAGGGCAA ACAGTCCTTT AGAAAGTATT GTTGAAGAAA CTTGTATTGA TTTGACCTCA 
GAGTCTCCTG GCTCCTGTGA AATCAAGAGA CATAATTTAA AGTCGGAGCC TCCATCAAAG 
TTGGATTGTT TAGAGTTGCC TGAAACTCTG GGTAATGGTC ACAAGAAGAG GAAAAACAGT 
CCTGGTGTTA GTCACTCTTC TCAGAAAAAA CAAAGAAAGG ACATAGACTT AAGTAGTGAA 
AAGACCCAGA GACTTAGTCC CAATTCTGAT AGAAATGGTG ATGCTCACAG AAAGCAAGCG 
AGCAAGAAAA GAGAACCTGC AGTAAATGAA ACGTCCTTGT CATCAGAGGC CAGCCCAGAG 
GTGAAGGGTT CAACAGCAGT ACTTGCTGCT TCCCCAGCAA GCCTTTCTGC AAAAAATGTT 
ATCAAAAAGA AGGGAGAAAT TATAGTTTCA TGGACAAGAA ATGATGACCG GGAAATTTTA 
CTGGAATGTC AGAAAAGAAT GCCGTCCCTG AAAACATTTA CTTATTTAGC TGTCAAGCTG 
AATAAAAATC CAAATCAGGT TTCAGAGAGG TTCCAGCAGC TGAAAAAGCT CTTTGAGAAG 
TCAAAATGCA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Casp8ap2 ( XM_006537949.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006538012.1 Mus musculus caspase 8 associated protein 2 (Casp8ap2), transcript variant X1, mRNA.

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CloneID	OMu32945
Clone ID Related Accession (Same CDS sequence)	XM_006537949.1
Accession Version	XM_006537949.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6021bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	CASP8-associated protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTACG CTCTGGGCGA AGGGTTGTGC CTGGCTGGGC CTCGAGGAGC CAGCTGCCGT 
CCTCGGGGAG CGCCCCTCGG AGCGTCTCGG GGGGCGCTGG GGCGAGCACC CGGAACATCG 
CCGGGTCTAC AGCGCGCCCC TCCCCTCCCC CACCCGTGTC TCCCAAGTCC CTACTGCCGA 
GCTCTCGCCT CTCCACTTAA AAATAATGAT GAAGGCTCAT TGGACATATA TGCTGGGTTG 
GACAGTGCTG TTTCTGACAG TACTGCTAGA TCCTGTGTGT CATTCAGAAA CTGTTTAGAT 
TTGTATGAAG AGATCCTGAC TGAAGAAGGA ACTGCGAAGG AGGCAACGTA CAATGACTTG 
CAGATAGAAT ATGGGAAATG TCAGCAGCAA ATGAAAGACC TGATGAAAAG GTTTAAGGAA 
ATACAGACAC AGAACTTGAA CTTAAAAAAT GAAAACCAGT CTCTTAAGAA GAATATCTCA 
GCACTTATCA AAACTGCCAG AGTGGAGATA AACCGTAAGG ATGAAGAGAT AAATCATCTT 
CACCAAAGAT TGTCTGAGTT TCCACATTTT CGAAATAACC ATAAAACTGC AAGAACAAAA 
GATTCACAGT CCACATCTCC CCATTTGGAT GATTGTTCAA AGACTGATCA CGGAGTTAAA 
AGTGATGTTC AGAAAGATGT ACATCCTAAC ACTGCACAGC CAAACTTGGA AAAGGAAGGA 
AAATCGCATT CTGAAGCACA GAATCCTTTG CACTTGTCTA CGGGTGTTGA GAAACATTGT 
GCCAATAATG TCTGGTCACG TTCTCCTTAC CAGGTTGGAG AAGGTAACTC AAATGAGGAT 
AATAGGAGAG GAAGGAGTGG CACTAGACAT AGCCAGTGTA GCAGAGGAAC TGATAGAACA 
CAGAAAGACT TACATAGCAG CTGTAATGAC AGTGAGCCAA GGGACAAGGA GGCTAACTCC 
AGACTACAAG GACACCCTGA GAAACATGGC AACAGTGAAG CAAGGACGGA GAGCAAAATT 
TCAGAGAGTA AAAGCAGCAC TGGTATGGGA TATAAAAGTG AGCGCAGTGC CTCTTCTTGG 
GAAAAAGAGA CTTCCAGAGA AAGGCCACAC ACTCGAGTGG AATCTCAACA TGACAAAAAT 
CTAGAGAAAC AAAATGAAAG ATTACAAAAT ATGCACAGAA AAGAGCTTCC GTCTCAGGAC 
AAAACAGAAA GAAAAGTTGA TGTGAAGTTT AAACCAGCAG GAGAGGAGCA GGGGCATCGG 
GGAAGAGTGG ACCGGGCGTT ACCTCCTCAT CCCAAGAATG ACGTGAAACA TTACGGCTTC 
AATAAGTATC ATCCAGAAGA GAGAAGGGGA AGGGAAGATT GTAAAAGAGA CAGAGGGATG 
AACAGTCATG GCTTTCAAGA TAGAAGATGT TCATCTTTTC TTTCAAGCAA CAGAAATAGC 
AAATACCCGC ACTCCAAGGA AGTTAGTGTT GCACACCAGT GGGAAAATAC TCCTTTCAAA 
GCAGAAAGAC ATAGAACTGA GGACAGGAGG AAAAGAGAAC GAGAAAACAA AGAAGAAAGT 
AGACATGTGA AAAGTGACAA AAAATCACCT CCAGAACACT TACAAAGGAC TCATAAAGAC 
ACTAAGAAAA GCACTGCTGA TGGAAAGAGA CAGACTGAGC CCAAACATGG TAAAGGTGCA 
GTCTCTAACA GTGAGCTTTC TAAAGGGACA GACAGTAAAG AAGGTGCAAC AAAGGTGGAG 
AGTGGGCCAA ATGAAGCAAA AGGCAAGGAC TTAAAGTTAA GCTTCATGGA AAAACTGAAC 
TTAACTCTTT CTCCTGCTAA AAAGCAGCCT GCTTGTCAAG ATAACCCACA TCAAATAACT 
GGTGTTCCCG AGCCCAGTGG CACGTGTGAC TCACGGTCCT TGGAGACGAC TGGAACGGTG 
GCATGCCTTC CTTCTGGCAG TGAACATAAT AGAGAGGAAA CCAAATCAGA GTTACCAGAG 
CCAAAGGAGG CTCTTTTGGC AACATCTCAA CTCAGGATCA GCATTCCAGA AAACAAAATG 
AAGGAAGAAA AGAGGTTGTT ATTTAAATCT GTTGAGAATA CTGTGCCTTG TGAACTGCTT 
GCTTGTGGCA CGGAAATTTC CCTCCCAGCA CCTGTAGAAA TTGAACAAGC AAGATGCTTG 
CTTGGATCAG TGGAAGTGGA AGAGACCTGT GGTGGTGCAA GGACAGCCGC TTCTGTGGTG 
ATGCATGTGT TACCAGAACA TGCTTCTGAA GATGCCAGCC AAGAATTGGA CACCAAAAGA 
CACGATGGTA TAAATGCTTG TGCTATTTCT GAAGGTGTGA AAACAAAGGT GATTCTTTCA 
CCAAAAGCAG CTGCAGCCAG TGAGAGCCAT CTTGCACCTT TGGTTGAAGA ACCTAGCATT 
TCACTAGTAA ACTGTTCGGG AGACAATAAT CCTAAACTTG AACCTTCTCT TGAAGAGAGG 
CCTATAGTTG AGACTAAATC CTGTCCTTTG GAGTCTTGTT TACCTAAAGA GACTTTTGTA 
CCTTCACCAC AGAAGACTGA GTTGATTGAC CACAAAATAG AAACTGGAGA ATCAAACTCA 
GTATATCAAG ATGATGATAA CTCAGTTTTG AGTATTGACT TTAATAATCT GAGACCTATT 
CCAGACCCCA TCAGCCCTCT GAATAGTCCA GTGAGACCGG TATGCAAAGT TGTTAGCATG 
GAGAGCTCAT GTGCAATTCC ACTGTATGAT AGCAGTCATA AAGATGAGTT TCCATCCAAT 
TCAACTCTTT CTACCTTCAA GAGTCAGTCT GATCTAAATA AGGAAAATGA AAAACCAGTT 
CCCAAATTTG ACAAATGTTC AGAAGCAGAT TCTTGTAAGC ATTTGTCTTT AGATGAATTA 
GAAGAAGGCG AAATTAGAAG TGATGATGAA GAATCTGTAG CACAAAAACG CTTGGAAAAG 
AGTGCAAGAC CTAGAGTTTC TGCTGAAGTG CAGCCAGGGA AAAGCAGCCC AGGAAGCAGG 
AGGAGCACTG TGCATGTGCA TAAGGACAAC GGGAGGACTG CTGTAAAACT CCCTCGGGAC 
AGACTCACAT GGAGCAAAAG ATCAAGTGAA TCAAGACCCT CCAACACCGA AAGGAAAAGT 
AAAACAATGA GCATCTCCAG CTTGGAAAAA ATACTTCCGC TTATCCTTGT ACCCTCTTCC 
CTGTGGGAGG TTATGCATAT GTTACGGTTG CTAGGTAAAC ATGTAAGAAA AAATTATATG 
AAATTCAAGA TAAAATTTTC ATTGACACAA TTTCATAGAA TTATTGAATC TGCAATTTTG 
AGTTTCACAT CACTAGTTAA ATGCCTTGAT TTGTCTAAGA TCTGTAAGTC AGTAAGTACT 
TTACAAAAGA GTCTTTGTGA AGTTATAGAA TCTAACCTTA AACAAGTGAA GAAGAATGGC 
ATAGTTGACC GTTTATTTGA ACAGCAACAA ACAGATATGA AAAAAAAATT GTGGAAGTTT 
GTAGATGAAC AACTTGATTA TTTGTTTGAA AAGCTTAAAA AAATCCTACT AAAGTTTTGT 
GATTCTGTAA ACTTTGAAAA TGAGAATAGT GAAGGAAAAC TTGGAAAAAA ATATAAAGAG 
AGAACCCAAC ATTCAAATTG TCAGAAGAAA AAAATGGACA ACAAAGAAAT ACGGAGAGAA 
AAAGTGCTAA AATCAGAAAA TACTGTGAAT TTTAAATCTT CACTGGGATG TGAAAAATCT 
GAAGAAAAAC ATCAGGACCA AAATAAAACC AATGCTAGTA TAGTAAAGCA TGATGTAAAA 
AGAACTTTTA GCACTTGCAG TGATAATACA AAGAACGCTG AATGTAAAGA GCAGTTTCTG 
GAAAAGAGCT GCCCGAGTAC CCCTAGGCCA GGAAAAGATG AAGGACATAC TGAGGAGGAG 
GCACAGGCGG CACAGCATGC AAGTGCTAAG TCCGAACGGA GCTTTGAGAT CCTTACTGAG 
CAGCAGGCAT CCAGCCTTAC TTTTAACTTA GTGAGTGATG CACAGATGGG TGAGATATTT 
AAAAGCTTGC TACAAGGTTC TGATCTGTTG GACACAAGTG GCACTGAAAA GGCAGAGTGG 
GAATTAAAGA CTCCAGAGAA ACAGCTGCTG GAAAGCCTCA AGTGTGAATC TGCACCAGCT 
TGTGCAACAG AAGAGCTGGT TTCAGAGGGG GCTTCTCTTT GTCCAAAAGT GATCAGTGAT 
GATAATTGGT CTTTATTATC ATCTGAAAAG GGTCCATCTT TATCTTCAGG GCTTTCACTG 
CCAGTTCATC CTGATGTGTT AGATGAAAAT TGTATGTTTG AAGTATCTTC TAACACTGCT 
TTAGGTAAAG ATAATGTATA CAGCTCAGAA AAGAGTAAGC CCTGCATCTC TTCCATACTC 
TTAGAAGATC TTGCGGTCTC TTTAACAGTA CCGTCACCTC TGAAATCAGA TGGCCATTTG 
AGTTTCTTAA AGCCAGAAGT TTTGTCAACT TCAACTCCTG AAGAAGTTAT TAGTGCACAT 
TTTAGTGAGG ATGCTTTGCT TGAGGAAGAG GATGCATCTG AACAGGACAT TCATCTAGCT 
CTGGAGTCTG ATAACTCAAG CAGTAAGTCA AGCTGTTCAT CATGGACAAG CCGGTCTGTT 
GCTTCAGGCT TTCAGTACCA CCCTAATCTT CCCATGCATG CTGTCATAAT GGAAAAGTCC 
AATGATCATT TCATTGTGAA AATACGGCGT GCAACACCAT CTACCTCCCC TGGCCTTAAA 
CATGGTGTGG TAGCTGAGGA GTCATTGACA TCTTTGCCTA GAACTGGAAA AGAAGCTGGT 
GTAGCAACAG AGAAAGAACC TAACCTGTTT CAGAGTACAG TTTTAAAACC TGTCAAGGAC 
TTGGAAAATA CTGATAAAAA TATTGATAAG AGTAAACTAA CTCATGAAGA ACAGAACTCT 
ATAGTACAAA CACAGGTTCC AGATATATAT GAATTTCTTA AAGATGCCTC AAATAAGGTG 
GTTCATTGTG ATCAAGTGGT TGATGATTGC TTCAAGTTGC ATCAAGTATG GGAACCAAAA 
GTTTCTGAGA ACCTTCAAGA GTTGCCTTCA ATGGAGAAAA TCCCACACTC TCTTGATAAT 
CATCTTCCTG ATACACACAT AGATCTAACG AAAGATTCAG CCACTGAGAC CAAAAGCTTG 
GGGGAGCTAA TGGAAGTAAC AGTTTTAAAT GTTGATCACT TGGAATGTTC TCAAACCAAC 
TTAGATCAAG ATGCAGAGAT AACATGTAGT TCTTTACAGC CTGATACTAT AGATGCTTTT 
ATTGATTTGA CACATGATGC TTCAAGTGAG AGTAAAAATG AAGGTAGTGA ACCTGTGTTA 
GCTGTTGAAG GTATGGGATG CCAGGTAATA TGTATAGATG AGGATACCAA CAAAGAAGGA 
AAGATGGGAA GGGCAAACAG TCCTTTAGAA AGTATTGTTG AAGAAACTTG TATTGATTTG 
ACCTCAGAGT CTCCTGGCTC CTGTGAAATC AAGAGACATA ATTTAAAGTC GGAGCCTCCA 
TCAAAGTTGG ATTGTTTAGA GTTGCCTGAA ACTCTGGGTA ATGGTCACAA GAAGAGGAAA 
AACAGTCCTG GTGTTAGTCA CTCTTCTCAG AAAAAACAAA GAAAGGACAT AGACTTAAGT 
AGTGAAAAGA CCCAGAGACT TAGTCCCAAT TCTGATAGAA ATGGTGATGC TCACAGAAAG 
CAAGCGAGCA AGAAAAGAGA ACCTGCAGTA AATGAAACGT CCTTGTCATC AGAGGCCAGC 
CCAGAGGTGA AGGGTTCAAC AGCAGTACTT GCTGCTTCCC CAGCAAGCCT TTCTGCAAAA 
AATGTTATCA AAAAGAAGGG AGAAATTATA GTTTCATGGA CAAGAAATGA TGACCGGGAA 
ATTTTACTGG AATGTCAGAA AAGAATGCCG TCCCTGAAAA CATTTACTTA TTTAGCTGTC 
AAGCTGAATA AAAATCCAAA TCAGGTTTCA GAGAGGTTCC AGCAGCTGAA AAAGCTCTTT 
GAGAAGTCAA AATGCAGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006538012.1
CDS	55..6075
Translation	MAYALGEGLCLAGPRGASCRPRGAPLGASRGALGRAPGTSPGLQRAPPLPHPCLPSPYCRALASPLKNNDEGSLDIYAGLDSAVSDSTARSCVSFRNCLDLYEEILTEEGTAKEATYNDLQIEYGKCQQQMKDLMKRFKEIQTQNLNLKNENQSLKKNISALIKTARVEINRKDEEINHLHQRLSEFPHFRNNHKTARTKDSQSTSPHLDDCSKTDHGVKSDVQKDVHPNTAQPNLEKEGKSHSEAQNPLHLSTGVEKHCANNVWSRSPYQVGEGNSNEDNRRGRSGTRHSQCSRGTDRTQKDLHSSCNDSEPRDKEANSRLQGHPEKHGNSEARTESKISESKSSTGMGYKSERSASSWEKETSRERPHTRVESQHDKNLEKQNERLQNMHRKELPSQDKTERKVDVKFKPAGEEQGHRGRVDRALPPHPKNDVKHYGFNKYHPEERRGREDCKRDRGMNSHGFQDRRCSSFLSSNRNSKYPHSKEVSVAHQWENTPFKAERHRTEDRRKRERENKEESRHVKSDKKSPPEHLQRTHKDTKKSTADGKRQTEPKHGKGAVSNSELSKGTDSKEGATKVESGPNEAKGKDLKLSFMEKLNLTLSPAKKQPACQDNPHQITGVPEPSGTCDSRSLETTGTVACLPSGSEHNREETKSELPEPKEALLATSQLRISIPENKMKEEKRLLFKSVENTVPCELLACGTEISLPAPVEIEQARCLLGSVEVEETCGGARTAASVVMHVLPEHASEDASQELDTKRHDGINACAISEGVKTKVILSPKAAAASESHLAPLVEEPSISLVNCSGDNNPKLEPSLEERPIVETKSCPLESCLPKETFVPSPQKTELIDHKIETGESNSVYQDDDNSVLSIDFNNLRPIPDPISPLNSPVRPVCKVVSMESSCAIPLYDSSHKDEFPSNSTLSTFKSQSDLNKENEKPVPKFDKCSEADSCKHLSLDELEEGEIRSDDEESVAQKRLEKSARPRVSAEVQPGKSSPGSRRSTVHVHKDNGRTAVKLPRDRLTWSKRSSESRPSNTERKSKTMSISSLEKILPLILVPSSLWEVMHMLRLLGKHVRKNYMKFKIKFSLTQFHRIIESAILSFTSLVKCLDLSKICKSVSTLQKSLCEVIESNLKQVKKNGIVDRLFEQQQTDMKKKLWKFVDEQLDYLFEKLKKILLKFCDSVNFENENSEGKLGKKYKERTQHSNCQKKKMDNKEIRREKVLKSENTVNFKSSLGCEKSEEKHQDQNKTNASIVKHDVKRTFSTCSDNTKNAECKEQFLEKSCPSTPRPGKDEGHTEEEAQAAQHASAKSERSFEILTEQQASSLTFNLVSDAQMGEIFKSLLQGSDLLDTSGTEKAEWELKTPEKQLLESLKCESAPACATEELVSEGASLCPKVISDDNWSLLSSEKGPSLSSGLSLPVHPDVLDENCMFEVSSNTALGKDNVYSSEKSKPCISSILLEDLAVSLTVPSPLKSDGHLSFLKPEVLSTSTPEEVISAHFSEDALLEEEDASEQDIHLALESDNSSSKSSCSSWTSRSVASGFQYHPNLPMHAVIMEKSNDHFIVKIRRATPSTSPGLKHGVVAEESLTSLPRTGKEAGVATEKEPNLFQSTVLKPVKDLENTDKNIDKSKLTHEEQNSIVQTQVPDIYEFLKDASNKVVHCDQVVDDCFKLHQVWEPKVSENLQELPSMEKIPHSLDNHLPDTHIDLTKDSATETKSLGELMEVTVLNVDHLECSQTNLDQDAEITCSSLQPDTIDAFIDLTHDASSESKNEGSEPVLAVEGMGCQVICIDEDTNKEGKMGRANSPLESIVEETCIDLTSESPGSCEIKRHNLKSEPPSKLDCLELPETLGNGHKKRKNSPGVSHSSQKKQRKDIDLSSEKTQRLSPNSDRNGDAHRKQASKKREPAVNETSLSSEASPEVKGSTAVLAASPASLSAKNVIKKKGEIIVSWTRNDDREILLECQKRMPSLKTFTYLAVKLNKNPNQVSERFQQLKKLFEKSKCR

Target ORF information:

RefSeq Version	XM_006537949.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus caspase 8 associated protein 2 (Casp8ap2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006537949.1

ORF Insert Sequence:

ATGGCTTACG CTCTGGGCGA AGGGTTGTGC CTGGCTGGGC CTCGAGGAGC CAGCTGCCGT 
CCTCGGGGAG CGCCCCTCGG AGCGTCTCGG GGGGCGCTGG GGCGAGCACC CGGAACATCG 
CCGGGTCTAC AGCGCGCCCC TCCCCTCCCC CACCCGTGTC TCCCAAGTCC CTACTGCCGA 
GCTCTCGCCT CTCCACTTAA AAATAATGAT GAAGGCTCAT TGGACATATA TGCTGGGTTG 
GACAGTGCTG TTTCTGACAG TACTGCTAGA TCCTGTGTGT CATTCAGAAA CTGTTTAGAT 
TTGTATGAAG AGATCCTGAC TGAAGAAGGA ACTGCGAAGG AGGCAACGTA CAATGACTTG 
CAGATAGAAT ATGGGAAATG TCAGCAGCAA ATGAAAGACC TGATGAAAAG GTTTAAGGAA 
ATACAGACAC AGAACTTGAA CTTAAAAAAT GAAAACCAGT CTCTTAAGAA GAATATCTCA 
GCACTTATCA AAACTGCCAG AGTGGAGATA AACCGTAAGG ATGAAGAGAT AAATCATCTT 
CACCAAAGAT TGTCTGAGTT TCCACATTTT CGAAATAACC ATAAAACTGC AAGAACAAAA 
GATTCACAGT CCACATCTCC CCATTTGGAT GATTGTTCAA AGACTGATCA CGGAGTTAAA 
AGTGATGTTC AGAAAGATGT ACATCCTAAC ACTGCACAGC CAAACTTGGA AAAGGAAGGA 
AAATCGCATT CTGAAGCACA GAATCCTTTG CACTTGTCTA CGGGTGTTGA GAAACATTGT 
GCCAATAATG TCTGGTCACG TTCTCCTTAC CAGGTTGGAG AAGGTAACTC AAATGAGGAT 
AATAGGAGAG GAAGGAGTGG CACTAGACAT AGCCAGTGTA GCAGAGGAAC TGATAGAACA 
CAGAAAGACT TACATAGCAG CTGTAATGAC AGTGAGCCAA GGGACAAGGA GGCTAACTCC 
AGACTACAAG GACACCCTGA GAAACATGGC AACAGTGAAG CAAGGACGGA GAGCAAAATT 
TCAGAGAGTA AAAGCAGCAC TGGTATGGGA TATAAAAGTG AGCGCAGTGC CTCTTCTTGG 
GAAAAAGAGA CTTCCAGAGA AAGGCCACAC ACTCGAGTGG AATCTCAACA TGACAAAAAT 
CTAGAGAAAC AAAATGAAAG ATTACAAAAT ATGCACAGAA AAGAGCTTCC GTCTCAGGAC 
AAAACAGAAA GAAAAGTTGA TGTGAAGTTT AAACCAGCAG GAGAGGAGCA GGGGCATCGG 
GGAAGAGTGG ACCGGGCGTT ACCTCCTCAT CCCAAGAATG ACGTGAAACA TTACGGCTTC 
AATAAGTATC ATCCAGAAGA GAGAAGGGGA AGGGAAGATT GTAAAAGAGA CAGAGGGATG 
AACAGTCATG GCTTTCAAGA TAGAAGATGT TCATCTTTTC TTTCAAGCAA CAGAAATAGC 
AAATACCCGC ACTCCAAGGA AGTTAGTGTT GCACACCAGT GGGAAAATAC TCCTTTCAAA 
GCAGAAAGAC ATAGAACTGA GGACAGGAGG AAAAGAGAAC GAGAAAACAA AGAAGAAAGT 
AGACATGTGA AAAGTGACAA AAAATCACCT CCAGAACACT TACAAAGGAC TCATAAAGAC 
ACTAAGAAAA GCACTGCTGA TGGAAAGAGA CAGACTGAGC CCAAACATGG TAAAGGTGCA 
GTCTCTAACA GTGAGCTTTC TAAAGGGACA GACAGTAAAG AAGGTGCAAC AAAGGTGGAG 
AGTGGGCCAA ATGAAGCAAA AGGCAAGGAC TTAAAGTTAA GCTTCATGGA AAAACTGAAC 
TTAACTCTTT CTCCTGCTAA AAAGCAGCCT GCTTGTCAAG ATAACCCACA TCAAATAACT 
GGTGTTCCCG AGCCCAGTGG CACGTGTGAC TCACGGTCCT TGGAGACGAC TGGAACGGTG 
GCATGCCTTC CTTCTGGCAG TGAACATAAT AGAGAGGAAA CCAAATCAGA GTTACCAGAG 
CCAAAGGAGG CTCTTTTGGC AACATCTCAA CTCAGGATCA GCATTCCAGA AAACAAAATG 
AAGGAAGAAA AGAGGTTGTT ATTTAAATCT GTTGAGAATA CTGTGCCTTG TGAACTGCTT 
GCTTGTGGCA CGGAAATTTC CCTCCCAGCA CCTGTAGAAA TTGAACAAGC AAGATGCTTG 
CTTGGATCAG TGGAAGTGGA AGAGACCTGT GGTGGTGCAA GGACAGCCGC TTCTGTGGTG 
ATGCATGTGT TACCAGAACA TGCTTCTGAA GATGCCAGCC AAGAATTGGA CACCAAAAGA 
CACGATGGTA TAAATGCTTG TGCTATTTCT GAAGGTGTGA AAACAAAGGT GATTCTTTCA 
CCAAAAGCAG CTGCAGCCAG TGAGAGCCAT CTTGCACCTT TGGTTGAAGA ACCTAGCATT 
TCACTAGTAA ACTGTTCGGG AGACAATAAT CCTAAACTTG AACCTTCTCT TGAAGAGAGG 
CCTATAGTTG AGACTAAATC CTGTCCTTTG GAGTCTTGTT TACCTAAAGA GACTTTTGTA 
CCTTCACCAC AGAAGACTGA GTTGATTGAC CACAAAATAG AAACTGGAGA ATCAAACTCA 
GTATATCAAG ATGATGATAA CTCAGTTTTG AGTATTGACT TTAATAATCT GAGACCTATT 
CCAGACCCCA TCAGCCCTCT GAATAGTCCA GTGAGACCGG TATGCAAAGT TGTTAGCATG 
GAGAGCTCAT GTGCAATTCC ACTGTATGAT AGCAGTCATA AAGATGAGTT TCCATCCAAT 
TCAACTCTTT CTACCTTCAA GAGTCAGTCT GATCTAAATA AGGAAAATGA AAAACCAGTT 
CCCAAATTTG ACAAATGTTC AGAAGCAGAT TCTTGTAAGC ATTTGTCTTT AGATGAATTA 
GAAGAAGGCG AAATTAGAAG TGATGATGAA GAATCTGTAG CACAAAAACG CTTGGAAAAG 
AGTGCAAGAC CTAGAGTTTC TGCTGAAGTG CAGCCAGGGA AAAGCAGCCC AGGAAGCAGG 
AGGAGCACTG TGCATGTGCA TAAGGACAAC GGGAGGACTG CTGTAAAACT CCCTCGGGAC 
AGACTCACAT GGAGCAAAAG ATCAAGTGAA TCAAGACCCT CCAACACCGA AAGGAAAAGT 
AAAACAATGA GCATCTCCAG CTTGGAAAAA ATACTTCCGC TTATCCTTGT ACCCTCTTCC 
CTGTGGGAGG TTATGCATAT GTTACGGTTG CTAGGTAAAC ATGTAAGAAA AAATTATATG 
AAATTCAAGA TAAAATTTTC ATTGACACAA TTTCATAGAA TTATTGAATC TGCAATTTTG 
AGTTTCACAT CACTAGTTAA ATGCCTTGAT TTGTCTAAGA TCTGTAAGTC AGTAAGTACT 
TTACAAAAGA GTCTTTGTGA AGTTATAGAA TCTAACCTTA AACAAGTGAA GAAGAATGGC 
ATAGTTGACC GTTTATTTGA ACAGCAACAA ACAGATATGA AAAAAAAATT GTGGAAGTTT 
GTAGATGAAC AACTTGATTA TTTGTTTGAA AAGCTTAAAA AAATCCTACT AAAGTTTTGT 
GATTCTGTAA ACTTTGAAAA TGAGAATAGT GAAGGAAAAC TTGGAAAAAA ATATAAAGAG 
AGAACCCAAC ATTCAAATTG TCAGAAGAAA AAAATGGACA ACAAAGAAAT ACGGAGAGAA 
AAAGTGCTAA AATCAGAAAA TACTGTGAAT TTTAAATCTT CACTGGGATG TGAAAAATCT 
GAAGAAAAAC ATCAGGACCA AAATAAAACC AATGCTAGTA TAGTAAAGCA TGATGTAAAA 
AGAACTTTTA GCACTTGCAG TGATAATACA AAGAACGCTG AATGTAAAGA GCAGTTTCTG 
GAAAAGAGCT GCCCGAGTAC CCCTAGGCCA GGAAAAGATG AAGGACATAC TGAGGAGGAG 
GCACAGGCGG CACAGCATGC AAGTGCTAAG TCCGAACGGA GCTTTGAGAT CCTTACTGAG 
CAGCAGGCAT CCAGCCTTAC TTTTAACTTA GTGAGTGATG CACAGATGGG TGAGATATTT 
AAAAGCTTGC TACAAGGTTC TGATCTGTTG GACACAAGTG GCACTGAAAA GGCAGAGTGG 
GAATTAAAGA CTCCAGAGAA ACAGCTGCTG GAAAGCCTCA AGTGTGAATC TGCACCAGCT 
TGTGCAACAG AAGAGCTGGT TTCAGAGGGG GCTTCTCTTT GTCCAAAAGT GATCAGTGAT 
GATAATTGGT CTTTATTATC ATCTGAAAAG GGTCCATCTT TATCTTCAGG GCTTTCACTG 
CCAGTTCATC CTGATGTGTT AGATGAAAAT TGTATGTTTG AAGTATCTTC TAACACTGCT 
TTAGGTAAAG ATAATGTATA CAGCTCAGAA AAGAGTAAGC CCTGCATCTC TTCCATACTC 
TTAGAAGATC TTGCGGTCTC TTTAACAGTA CCGTCACCTC TGAAATCAGA TGGCCATTTG 
AGTTTCTTAA AGCCAGAAGT TTTGTCAACT TCAACTCCTG AAGAAGTTAT TAGTGCACAT 
TTTAGTGAGG ATGCTTTGCT TGAGGAAGAG GATGCATCTG AACAGGACAT TCATCTAGCT 
CTGGAGTCTG ATAACTCAAG CAGTAAGTCA AGCTGTTCAT CATGGACAAG CCGGTCTGTT 
GCTTCAGGCT TTCAGTACCA CCCTAATCTT CCCATGCATG CTGTCATAAT GGAAAAGTCC 
AATGATCATT TCATTGTGAA AATACGGCGT GCAACACCAT CTACCTCCCC TGGCCTTAAA 
CATGGTGTGG TAGCTGAGGA GTCATTGACA TCTTTGCCTA GAACTGGAAA AGAAGCTGGT 
GTAGCAACAG AGAAAGAACC TAACCTGTTT CAGAGTACAG TTTTAAAACC TGTCAAGGAC 
TTGGAAAATA CTGATAAAAA TATTGATAAG AGTAAACTAA CTCATGAAGA ACAGAACTCT 
ATAGTACAAA CACAGGTTCC AGATATATAT GAATTTCTTA AAGATGCCTC AAATAAGGTG 
GTTCATTGTG ATCAAGTGGT TGATGATTGC TTCAAGTTGC ATCAAGTATG GGAACCAAAA 
GTTTCTGAGA ACCTTCAAGA GTTGCCTTCA ATGGAGAAAA TCCCACACTC TCTTGATAAT 
CATCTTCCTG ATACACACAT AGATCTAACG AAAGATTCAG CCACTGAGAC CAAAAGCTTG 
GGGGAGCTAA TGGAAGTAAC AGTTTTAAAT GTTGATCACT TGGAATGTTC TCAAACCAAC 
TTAGATCAAG ATGCAGAGAT AACATGTAGT TCTTTACAGC CTGATACTAT AGATGCTTTT 
ATTGATTTGA CACATGATGC TTCAAGTGAG AGTAAAAATG AAGGTAGTGA ACCTGTGTTA 
GCTGTTGAAG GTATGGGATG CCAGGTAATA TGTATAGATG AGGATACCAA CAAAGAAGGA 
AAGATGGGAA GGGCAAACAG TCCTTTAGAA AGTATTGTTG AAGAAACTTG TATTGATTTG 
ACCTCAGAGT CTCCTGGCTC CTGTGAAATC AAGAGACATA ATTTAAAGTC GGAGCCTCCA 
TCAAAGTTGG ATTGTTTAGA GTTGCCTGAA ACTCTGGGTA ATGGTCACAA GAAGAGGAAA 
AACAGTCCTG GTGTTAGTCA CTCTTCTCAG AAAAAACAAA GAAAGGACAT AGACTTAAGT 
AGTGAAAAGA CCCAGAGACT TAGTCCCAAT TCTGATAGAA ATGGTGATGC TCACAGAAAG 
CAAGCGAGCA AGAAAAGAGA ACCTGCAGTA AATGAAACGT CCTTGTCATC AGAGGCCAGC 
CCAGAGGTGA AGGGTTCAAC AGCAGTACTT GCTGCTTCCC CAGCAAGCCT TTCTGCAAAA 
AATGTTATCA AAAAGAAGGG AGAAATTATA GTTTCATGGA CAAGAAATGA TGACCGGGAA 
ATTTTACTGG AATGTCAGAA AAGAATGCCG TCCCTGAAAA CATTTACTTA TTTAGCTGTC 
AAGCTGAATA AAAATCCAAA TCAGGTTTCA GAGAGGTTCC AGCAGCTGAA AAAGCTCTTT 
GAGAAGTCAA AATGCAGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Casp8ap2 ( XM_006537950.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006538013.1 Mus musculus caspase 8 associated protein 2 (Casp8ap2), transcript variant X2, mRNA.

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CloneID	OMu32946
Clone ID Related Accession (Same CDS sequence)	XM_006537950.1 , XM_006537950.2
Accession Version	XM_006537950.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6015bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	CASP8-associated protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTACG CTCTGGGCGA AGGGTTGTGC CTGGCTGGGC CTCGAGGAGC CAGCTGCCGT 
CCTCGGGGAG CGCCCCTCGG AGCGTCTCGG GGGGCGCTGG GGCGAGCACC CGGAACATCG 
CCGGGTCTAC AGCGCGCCCC TCCCCTCCCC CACCCGTGTC TCCCAAGTCC CTACTGCCGA 
GCTCTCGCCT CTCCACTTAA AAATAATGAT GAAGGCTCAT TGGACATATA TGCTGGGTTG 
GACAGTGCTG TTTCTGACAG TACTGCTAGA TCCTGTGTGT CATTCAGAAA CTGTTTAGAT 
TTGTATGAAG AGATCCTGAC TGAAGAAGGA ACTGCGAAGG AGGCAACGTA CAATGACATA 
GAATATGGGA AATGTCAGCA GCAAATGAAA GACCTGATGA AAAGGTTTAA GGAAATACAG 
ACACAGAACT TGAACTTAAA AAATGAAAAC CAGTCTCTTA AGAAGAATAT CTCAGCACTT 
ATCAAAACTG CCAGAGTGGA GATAAACCGT AAGGATGAAG AGATAAATCA TCTTCACCAA 
AGATTGTCTG AGTTTCCACA TTTTCGAAAT AACCATAAAA CTGCAAGAAC AAAAGATTCA 
CAGTCCACAT CTCCCCATTT GGATGATTGT TCAAAGACTG ATCACGGAGT TAAAAGTGAT 
GTTCAGAAAG ATGTACATCC TAACACTGCA CAGCCAAACT TGGAAAAGGA AGGAAAATCG 
CATTCTGAAG CACAGAATCC TTTGCACTTG TCTACGGGTG TTGAGAAACA TTGTGCCAAT 
AATGTCTGGT CACGTTCTCC TTACCAGGTT GGAGAAGGTA ACTCAAATGA GGATAATAGG 
AGAGGAAGGA GTGGCACTAG ACATAGCCAG TGTAGCAGAG GAACTGATAG AACACAGAAA 
GACTTACATA GCAGCTGTAA TGACAGTGAG CCAAGGGACA AGGAGGCTAA CTCCAGACTA 
CAAGGACACC CTGAGAAACA TGGCAACAGT GAAGCAAGGA CGGAGAGCAA AATTTCAGAG 
AGTAAAAGCA GCACTGGTAT GGGATATAAA AGTGAGCGCA GTGCCTCTTC TTGGGAAAAA 
GAGACTTCCA GAGAAAGGCC ACACACTCGA GTGGAATCTC AACATGACAA AAATCTAGAG 
AAACAAAATG AAAGATTACA AAATATGCAC AGAAAAGAGC TTCCGTCTCA GGACAAAACA 
GAAAGAAAAG TTGATGTGAA GTTTAAACCA GCAGGAGAGG AGCAGGGGCA TCGGGGAAGA 
GTGGACCGGG CGTTACCTCC TCATCCCAAG AATGACGTGA AACATTACGG CTTCAATAAG 
TATCATCCAG AAGAGAGAAG GGGAAGGGAA GATTGTAAAA GAGACAGAGG GATGAACAGT 
CATGGCTTTC AAGATAGAAG ATGTTCATCT TTTCTTTCAA GCAACAGAAA TAGCAAATAC 
CCGCACTCCA AGGAAGTTAG TGTTGCACAC CAGTGGGAAA ATACTCCTTT CAAAGCAGAA 
AGACATAGAA CTGAGGACAG GAGGAAAAGA GAACGAGAAA ACAAAGAAGA AAGTAGACAT 
GTGAAAAGTG ACAAAAAATC ACCTCCAGAA CACTTACAAA GGACTCATAA AGACACTAAG 
AAAAGCACTG CTGATGGAAA GAGACAGACT GAGCCCAAAC ATGGTAAAGG TGCAGTCTCT 
AACAGTGAGC TTTCTAAAGG GACAGACAGT AAAGAAGGTG CAACAAAGGT GGAGAGTGGG 
CCAAATGAAG CAAAAGGCAA GGACTTAAAG TTAAGCTTCA TGGAAAAACT GAACTTAACT 
CTTTCTCCTG CTAAAAAGCA GCCTGCTTGT CAAGATAACC CACATCAAAT AACTGGTGTT 
CCCGAGCCCA GTGGCACGTG TGACTCACGG TCCTTGGAGA CGACTGGAAC GGTGGCATGC 
CTTCCTTCTG GCAGTGAACA TAATAGAGAG GAAACCAAAT CAGAGTTACC AGAGCCAAAG 
GAGGCTCTTT TGGCAACATC TCAACTCAGG ATCAGCATTC CAGAAAACAA AATGAAGGAA 
GAAAAGAGGT TGTTATTTAA ATCTGTTGAG AATACTGTGC CTTGTGAACT GCTTGCTTGT 
GGCACGGAAA TTTCCCTCCC AGCACCTGTA GAAATTGAAC AAGCAAGATG CTTGCTTGGA 
TCAGTGGAAG TGGAAGAGAC CTGTGGTGGT GCAAGGACAG CCGCTTCTGT GGTGATGCAT 
GTGTTACCAG AACATGCTTC TGAAGATGCC AGCCAAGAAT TGGACACCAA AAGACACGAT 
GGTATAAATG CTTGTGCTAT TTCTGAAGGT GTGAAAACAA AGGTGATTCT TTCACCAAAA 
GCAGCTGCAG CCAGTGAGAG CCATCTTGCA CCTTTGGTTG AAGAACCTAG CATTTCACTA 
GTAAACTGTT CGGGAGACAA TAATCCTAAA CTTGAACCTT CTCTTGAAGA GAGGCCTATA 
GTTGAGACTA AATCCTGTCC TTTGGAGTCT TGTTTACCTA AAGAGACTTT TGTACCTTCA 
CCACAGAAGA CTGAGTTGAT TGACCACAAA ATAGAAACTG GAGAATCAAA CTCAGTATAT 
CAAGATGATG ATAACTCAGT TTTGAGTATT GACTTTAATA ATCTGAGACC TATTCCAGAC 
CCCATCAGCC CTCTGAATAG TCCAGTGAGA CCGGTATGCA AAGTTGTTAG CATGGAGAGC 
TCATGTGCAA TTCCACTGTA TGATAGCAGT CATAAAGATG AGTTTCCATC CAATTCAACT 
CTTTCTACCT TCAAGAGTCA GTCTGATCTA AATAAGGAAA ATGAAAAACC AGTTCCCAAA 
TTTGACAAAT GTTCAGAAGC AGATTCTTGT AAGCATTTGT CTTTAGATGA ATTAGAAGAA 
GGCGAAATTA GAAGTGATGA TGAAGAATCT GTAGCACAAA AACGCTTGGA AAAGAGTGCA 
AGACCTAGAG TTTCTGCTGA AGTGCAGCCA GGGAAAAGCA GCCCAGGAAG CAGGAGGAGC 
ACTGTGCATG TGCATAAGGA CAACGGGAGG ACTGCTGTAA AACTCCCTCG GGACAGACTC 
ACATGGAGCA AAAGATCAAG TGAATCAAGA CCCTCCAACA CCGAAAGGAA AAGTAAAACA 
ATGAGCATCT CCAGCTTGGA AAAAATACTT CCGCTTATCC TTGTACCCTC TTCCCTGTGG 
GAGGTTATGC ATATGTTACG GTTGCTAGGT AAACATGTAA GAAAAAATTA TATGAAATTC 
AAGATAAAAT TTTCATTGAC ACAATTTCAT AGAATTATTG AATCTGCAAT TTTGAGTTTC 
ACATCACTAG TTAAATGCCT TGATTTGTCT AAGATCTGTA AGTCAGTAAG TACTTTACAA 
AAGAGTCTTT GTGAAGTTAT AGAATCTAAC CTTAAACAAG TGAAGAAGAA TGGCATAGTT 
GACCGTTTAT TTGAACAGCA ACAAACAGAT ATGAAAAAAA AATTGTGGAA GTTTGTAGAT 
GAACAACTTG ATTATTTGTT TGAAAAGCTT AAAAAAATCC TACTAAAGTT TTGTGATTCT 
GTAAACTTTG AAAATGAGAA TAGTGAAGGA AAACTTGGAA AAAAATATAA AGAGAGAACC 
CAACATTCAA ATTGTCAGAA GAAAAAAATG GACAACAAAG AAATACGGAG AGAAAAAGTG 
CTAAAATCAG AAAATACTGT GAATTTTAAA TCTTCACTGG GATGTGAAAA ATCTGAAGAA 
AAACATCAGG ACCAAAATAA AACCAATGCT AGTATAGTAA AGCATGATGT AAAAAGAACT 
TTTAGCACTT GCAGTGATAA TACAAAGAAC GCTGAATGTA AAGAGCAGTT TCTGGAAAAG 
AGCTGCCCGA GTACCCCTAG GCCAGGAAAA GATGAAGGAC ATACTGAGGA GGAGGCACAG 
GCGGCACAGC ATGCAAGTGC TAAGTCCGAA CGGAGCTTTG AGATCCTTAC TGAGCAGCAG 
GCATCCAGCC TTACTTTTAA CTTAGTGAGT GATGCACAGA TGGGTGAGAT ATTTAAAAGC 
TTGCTACAAG GTTCTGATCT GTTGGACACA AGTGGCACTG AAAAGGCAGA GTGGGAATTA 
AAGACTCCAG AGAAACAGCT GCTGGAAAGC CTCAAGTGTG AATCTGCACC AGCTTGTGCA 
ACAGAAGAGC TGGTTTCAGA GGGGGCTTCT CTTTGTCCAA AAGTGATCAG TGATGATAAT 
TGGTCTTTAT TATCATCTGA AAAGGGTCCA TCTTTATCTT CAGGGCTTTC ACTGCCAGTT 
CATCCTGATG TGTTAGATGA AAATTGTATG TTTGAAGTAT CTTCTAACAC TGCTTTAGGT 
AAAGATAATG TATACAGCTC AGAAAAGAGT AAGCCCTGCA TCTCTTCCAT ACTCTTAGAA 
GATCTTGCGG TCTCTTTAAC AGTACCGTCA CCTCTGAAAT CAGATGGCCA TTTGAGTTTC 
TTAAAGCCAG AAGTTTTGTC AACTTCAACT CCTGAAGAAG TTATTAGTGC ACATTTTAGT 
GAGGATGCTT TGCTTGAGGA AGAGGATGCA TCTGAACAGG ACATTCATCT AGCTCTGGAG 
TCTGATAACT CAAGCAGTAA GTCAAGCTGT TCATCATGGA CAAGCCGGTC TGTTGCTTCA 
GGCTTTCAGT ACCACCCTAA TCTTCCCATG CATGCTGTCA TAATGGAAAA GTCCAATGAT 
CATTTCATTG TGAAAATACG GCGTGCAACA CCATCTACCT CCCCTGGCCT TAAACATGGT 
GTGGTAGCTG AGGAGTCATT GACATCTTTG CCTAGAACTG GAAAAGAAGC TGGTGTAGCA 
ACAGAGAAAG AACCTAACCT GTTTCAGAGT ACAGTTTTAA AACCTGTCAA GGACTTGGAA 
AATACTGATA AAAATATTGA TAAGAGTAAA CTAACTCATG AAGAACAGAA CTCTATAGTA 
CAAACACAGG TTCCAGATAT ATATGAATTT CTTAAAGATG CCTCAAATAA GGTGGTTCAT 
TGTGATCAAG TGGTTGATGA TTGCTTCAAG TTGCATCAAG TATGGGAACC AAAAGTTTCT 
GAGAACCTTC AAGAGTTGCC TTCAATGGAG AAAATCCCAC ACTCTCTTGA TAATCATCTT 
CCTGATACAC ACATAGATCT AACGAAAGAT TCAGCCACTG AGACCAAAAG CTTGGGGGAG 
CTAATGGAAG TAACAGTTTT AAATGTTGAT CACTTGGAAT GTTCTCAAAC CAACTTAGAT 
CAAGATGCAG AGATAACATG TAGTTCTTTA CAGCCTGATA CTATAGATGC TTTTATTGAT 
TTGACACATG ATGCTTCAAG TGAGAGTAAA AATGAAGGTA GTGAACCTGT GTTAGCTGTT 
GAAGGTATGG GATGCCAGGT AATATGTATA GATGAGGATA CCAACAAAGA AGGAAAGATG 
GGAAGGGCAA ACAGTCCTTT AGAAAGTATT GTTGAAGAAA CTTGTATTGA TTTGACCTCA 
GAGTCTCCTG GCTCCTGTGA AATCAAGAGA CATAATTTAA AGTCGGAGCC TCCATCAAAG 
TTGGATTGTT TAGAGTTGCC TGAAACTCTG GGTAATGGTC ACAAGAAGAG GAAAAACAGT 
CCTGGTGTTA GTCACTCTTC TCAGAAAAAA CAAAGAAAGG ACATAGACTT AAGTAGTGAA 
AAGACCCAGA GACTTAGTCC CAATTCTGAT AGAAATGGTG ATGCTCACAG AAAGCAAGCG 
AGCAAGAAAA GAGAACCTGC AGTAAATGAA ACGTCCTTGT CATCAGAGGC CAGCCCAGAG 
GTGAAGGGTT CAACAGCAGT ACTTGCTGCT TCCCCAGCAA GCCTTTCTGC AAAAAATGTT 
ATCAAAAAGA AGGGAGAAAT TATAGTTTCA TGGACAAGAA ATGATGACCG GGAAATTTTA 
CTGGAATGTC AGAAAAGAAT GCCGTCCCTG AAAACATTTA CTTATTTAGC TGTCAAGCTG 
AATAAAAATC CAAATCAGGT TTCAGAGAGG TTCCAGCAGC TGAAAAAGCT CTTTGAGAAG 
TCAAAATGCA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006538013.1
CDS	55..6069
Translation	MAYALGEGLCLAGPRGASCRPRGAPLGASRGALGRAPGTSPGLQRAPPLPHPCLPSPYCRALASPLKNNDEGSLDIYAGLDSAVSDSTARSCVSFRNCLDLYEEILTEEGTAKEATYNDIEYGKCQQQMKDLMKRFKEIQTQNLNLKNENQSLKKNISALIKTARVEINRKDEEINHLHQRLSEFPHFRNNHKTARTKDSQSTSPHLDDCSKTDHGVKSDVQKDVHPNTAQPNLEKEGKSHSEAQNPLHLSTGVEKHCANNVWSRSPYQVGEGNSNEDNRRGRSGTRHSQCSRGTDRTQKDLHSSCNDSEPRDKEANSRLQGHPEKHGNSEARTESKISESKSSTGMGYKSERSASSWEKETSRERPHTRVESQHDKNLEKQNERLQNMHRKELPSQDKTERKVDVKFKPAGEEQGHRGRVDRALPPHPKNDVKHYGFNKYHPEERRGREDCKRDRGMNSHGFQDRRCSSFLSSNRNSKYPHSKEVSVAHQWENTPFKAERHRTEDRRKRERENKEESRHVKSDKKSPPEHLQRTHKDTKKSTADGKRQTEPKHGKGAVSNSELSKGTDSKEGATKVESGPNEAKGKDLKLSFMEKLNLTLSPAKKQPACQDNPHQITGVPEPSGTCDSRSLETTGTVACLPSGSEHNREETKSELPEPKEALLATSQLRISIPENKMKEEKRLLFKSVENTVPCELLACGTEISLPAPVEIEQARCLLGSVEVEETCGGARTAASVVMHVLPEHASEDASQELDTKRHDGINACAISEGVKTKVILSPKAAAASESHLAPLVEEPSISLVNCSGDNNPKLEPSLEERPIVETKSCPLESCLPKETFVPSPQKTELIDHKIETGESNSVYQDDDNSVLSIDFNNLRPIPDPISPLNSPVRPVCKVVSMESSCAIPLYDSSHKDEFPSNSTLSTFKSQSDLNKENEKPVPKFDKCSEADSCKHLSLDELEEGEIRSDDEESVAQKRLEKSARPRVSAEVQPGKSSPGSRRSTVHVHKDNGRTAVKLPRDRLTWSKRSSESRPSNTERKSKTMSISSLEKILPLILVPSSLWEVMHMLRLLGKHVRKNYMKFKIKFSLTQFHRIIESAILSFTSLVKCLDLSKICKSVSTLQKSLCEVIESNLKQVKKNGIVDRLFEQQQTDMKKKLWKFVDEQLDYLFEKLKKILLKFCDSVNFENENSEGKLGKKYKERTQHSNCQKKKMDNKEIRREKVLKSENTVNFKSSLGCEKSEEKHQDQNKTNASIVKHDVKRTFSTCSDNTKNAECKEQFLEKSCPSTPRPGKDEGHTEEEAQAAQHASAKSERSFEILTEQQASSLTFNLVSDAQMGEIFKSLLQGSDLLDTSGTEKAEWELKTPEKQLLESLKCESAPACATEELVSEGASLCPKVISDDNWSLLSSEKGPSLSSGLSLPVHPDVLDENCMFEVSSNTALGKDNVYSSEKSKPCISSILLEDLAVSLTVPSPLKSDGHLSFLKPEVLSTSTPEEVISAHFSEDALLEEEDASEQDIHLALESDNSSSKSSCSSWTSRSVASGFQYHPNLPMHAVIMEKSNDHFIVKIRRATPSTSPGLKHGVVAEESLTSLPRTGKEAGVATEKEPNLFQSTVLKPVKDLENTDKNIDKSKLTHEEQNSIVQTQVPDIYEFLKDASNKVVHCDQVVDDCFKLHQVWEPKVSENLQELPSMEKIPHSLDNHLPDTHIDLTKDSATETKSLGELMEVTVLNVDHLECSQTNLDQDAEITCSSLQPDTIDAFIDLTHDASSESKNEGSEPVLAVEGMGCQVICIDEDTNKEGKMGRANSPLESIVEETCIDLTSESPGSCEIKRHNLKSEPPSKLDCLELPETLGNGHKKRKNSPGVSHSSQKKQRKDIDLSSEKTQRLSPNSDRNGDAHRKQASKKREPAVNETSLSSEASPEVKGSTAVLAASPASLSAKNVIKKKGEIIVSWTRNDDREILLECQKRMPSLKTFTYLAVKLNKNPNQVSERFQQLKKLFEKSKCR

Target ORF information:

RefSeq Version	XM_006537950.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus caspase 8 associated protein 2 (Casp8ap2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006537950.1

ORF Insert Sequence:

ATGGCTTACG CTCTGGGCGA AGGGTTGTGC CTGGCTGGGC CTCGAGGAGC CAGCTGCCGT 
CCTCGGGGAG CGCCCCTCGG AGCGTCTCGG GGGGCGCTGG GGCGAGCACC CGGAACATCG 
CCGGGTCTAC AGCGCGCCCC TCCCCTCCCC CACCCGTGTC TCCCAAGTCC CTACTGCCGA 
GCTCTCGCCT CTCCACTTAA AAATAATGAT GAAGGCTCAT TGGACATATA TGCTGGGTTG 
GACAGTGCTG TTTCTGACAG TACTGCTAGA TCCTGTGTGT CATTCAGAAA CTGTTTAGAT 
TTGTATGAAG AGATCCTGAC TGAAGAAGGA ACTGCGAAGG AGGCAACGTA CAATGACATA 
GAATATGGGA AATGTCAGCA GCAAATGAAA GACCTGATGA AAAGGTTTAA GGAAATACAG 
ACACAGAACT TGAACTTAAA AAATGAAAAC CAGTCTCTTA AGAAGAATAT CTCAGCACTT 
ATCAAAACTG CCAGAGTGGA GATAAACCGT AAGGATGAAG AGATAAATCA TCTTCACCAA 
AGATTGTCTG AGTTTCCACA TTTTCGAAAT AACCATAAAA CTGCAAGAAC AAAAGATTCA 
CAGTCCACAT CTCCCCATTT GGATGATTGT TCAAAGACTG ATCACGGAGT TAAAAGTGAT 
GTTCAGAAAG ATGTACATCC TAACACTGCA CAGCCAAACT TGGAAAAGGA AGGAAAATCG 
CATTCTGAAG CACAGAATCC TTTGCACTTG TCTACGGGTG TTGAGAAACA TTGTGCCAAT 
AATGTCTGGT CACGTTCTCC TTACCAGGTT GGAGAAGGTA ACTCAAATGA GGATAATAGG 
AGAGGAAGGA GTGGCACTAG ACATAGCCAG TGTAGCAGAG GAACTGATAG AACACAGAAA 
GACTTACATA GCAGCTGTAA TGACAGTGAG CCAAGGGACA AGGAGGCTAA CTCCAGACTA 
CAAGGACACC CTGAGAAACA TGGCAACAGT GAAGCAAGGA CGGAGAGCAA AATTTCAGAG 
AGTAAAAGCA GCACTGGTAT GGGATATAAA AGTGAGCGCA GTGCCTCTTC TTGGGAAAAA 
GAGACTTCCA GAGAAAGGCC ACACACTCGA GTGGAATCTC AACATGACAA AAATCTAGAG 
AAACAAAATG AAAGATTACA AAATATGCAC AGAAAAGAGC TTCCGTCTCA GGACAAAACA 
GAAAGAAAAG TTGATGTGAA GTTTAAACCA GCAGGAGAGG AGCAGGGGCA TCGGGGAAGA 
GTGGACCGGG CGTTACCTCC TCATCCCAAG AATGACGTGA AACATTACGG CTTCAATAAG 
TATCATCCAG AAGAGAGAAG GGGAAGGGAA GATTGTAAAA GAGACAGAGG GATGAACAGT 
CATGGCTTTC AAGATAGAAG ATGTTCATCT TTTCTTTCAA GCAACAGAAA TAGCAAATAC 
CCGCACTCCA AGGAAGTTAG TGTTGCACAC CAGTGGGAAA ATACTCCTTT CAAAGCAGAA 
AGACATAGAA CTGAGGACAG GAGGAAAAGA GAACGAGAAA ACAAAGAAGA AAGTAGACAT 
GTGAAAAGTG ACAAAAAATC ACCTCCAGAA CACTTACAAA GGACTCATAA AGACACTAAG 
AAAAGCACTG CTGATGGAAA GAGACAGACT GAGCCCAAAC ATGGTAAAGG TGCAGTCTCT 
AACAGTGAGC TTTCTAAAGG GACAGACAGT AAAGAAGGTG CAACAAAGGT GGAGAGTGGG 
CCAAATGAAG CAAAAGGCAA GGACTTAAAG TTAAGCTTCA TGGAAAAACT GAACTTAACT 
CTTTCTCCTG CTAAAAAGCA GCCTGCTTGT CAAGATAACC CACATCAAAT AACTGGTGTT 
CCCGAGCCCA GTGGCACGTG TGACTCACGG TCCTTGGAGA CGACTGGAAC GGTGGCATGC 
CTTCCTTCTG GCAGTGAACA TAATAGAGAG GAAACCAAAT CAGAGTTACC AGAGCCAAAG 
GAGGCTCTTT TGGCAACATC TCAACTCAGG ATCAGCATTC CAGAAAACAA AATGAAGGAA 
GAAAAGAGGT TGTTATTTAA ATCTGTTGAG AATACTGTGC CTTGTGAACT GCTTGCTTGT 
GGCACGGAAA TTTCCCTCCC AGCACCTGTA GAAATTGAAC AAGCAAGATG CTTGCTTGGA 
TCAGTGGAAG TGGAAGAGAC CTGTGGTGGT GCAAGGACAG CCGCTTCTGT GGTGATGCAT 
GTGTTACCAG AACATGCTTC TGAAGATGCC AGCCAAGAAT TGGACACCAA AAGACACGAT 
GGTATAAATG CTTGTGCTAT TTCTGAAGGT GTGAAAACAA AGGTGATTCT TTCACCAAAA 
GCAGCTGCAG CCAGTGAGAG CCATCTTGCA CCTTTGGTTG AAGAACCTAG CATTTCACTA 
GTAAACTGTT CGGGAGACAA TAATCCTAAA CTTGAACCTT CTCTTGAAGA GAGGCCTATA 
GTTGAGACTA AATCCTGTCC TTTGGAGTCT TGTTTACCTA AAGAGACTTT TGTACCTTCA 
CCACAGAAGA CTGAGTTGAT TGACCACAAA ATAGAAACTG GAGAATCAAA CTCAGTATAT 
CAAGATGATG ATAACTCAGT TTTGAGTATT GACTTTAATA ATCTGAGACC TATTCCAGAC 
CCCATCAGCC CTCTGAATAG TCCAGTGAGA CCGGTATGCA AAGTTGTTAG CATGGAGAGC 
TCATGTGCAA TTCCACTGTA TGATAGCAGT CATAAAGATG AGTTTCCATC CAATTCAACT 
CTTTCTACCT TCAAGAGTCA GTCTGATCTA AATAAGGAAA ATGAAAAACC AGTTCCCAAA 
TTTGACAAAT GTTCAGAAGC AGATTCTTGT AAGCATTTGT CTTTAGATGA ATTAGAAGAA 
GGCGAAATTA GAAGTGATGA TGAAGAATCT GTAGCACAAA AACGCTTGGA AAAGAGTGCA 
AGACCTAGAG TTTCTGCTGA AGTGCAGCCA GGGAAAAGCA GCCCAGGAAG CAGGAGGAGC 
ACTGTGCATG TGCATAAGGA CAACGGGAGG ACTGCTGTAA AACTCCCTCG GGACAGACTC 
ACATGGAGCA AAAGATCAAG TGAATCAAGA CCCTCCAACA CCGAAAGGAA AAGTAAAACA 
ATGAGCATCT CCAGCTTGGA AAAAATACTT CCGCTTATCC TTGTACCCTC TTCCCTGTGG 
GAGGTTATGC ATATGTTACG GTTGCTAGGT AAACATGTAA GAAAAAATTA TATGAAATTC 
AAGATAAAAT TTTCATTGAC ACAATTTCAT AGAATTATTG AATCTGCAAT TTTGAGTTTC 
ACATCACTAG TTAAATGCCT TGATTTGTCT AAGATCTGTA AGTCAGTAAG TACTTTACAA 
AAGAGTCTTT GTGAAGTTAT AGAATCTAAC CTTAAACAAG TGAAGAAGAA TGGCATAGTT 
GACCGTTTAT TTGAACAGCA ACAAACAGAT ATGAAAAAAA AATTGTGGAA GTTTGTAGAT 
GAACAACTTG ATTATTTGTT TGAAAAGCTT AAAAAAATCC TACTAAAGTT TTGTGATTCT 
GTAAACTTTG AAAATGAGAA TAGTGAAGGA AAACTTGGAA AAAAATATAA AGAGAGAACC 
CAACATTCAA ATTGTCAGAA GAAAAAAATG GACAACAAAG AAATACGGAG AGAAAAAGTG 
CTAAAATCAG AAAATACTGT GAATTTTAAA TCTTCACTGG GATGTGAAAA ATCTGAAGAA 
AAACATCAGG ACCAAAATAA AACCAATGCT AGTATAGTAA AGCATGATGT AAAAAGAACT 
TTTAGCACTT GCAGTGATAA TACAAAGAAC GCTGAATGTA AAGAGCAGTT TCTGGAAAAG 
AGCTGCCCGA GTACCCCTAG GCCAGGAAAA GATGAAGGAC ATACTGAGGA GGAGGCACAG 
GCGGCACAGC ATGCAAGTGC TAAGTCCGAA CGGAGCTTTG AGATCCTTAC TGAGCAGCAG 
GCATCCAGCC TTACTTTTAA CTTAGTGAGT GATGCACAGA TGGGTGAGAT ATTTAAAAGC 
TTGCTACAAG GTTCTGATCT GTTGGACACA AGTGGCACTG AAAAGGCAGA GTGGGAATTA 
AAGACTCCAG AGAAACAGCT GCTGGAAAGC CTCAAGTGTG AATCTGCACC AGCTTGTGCA 
ACAGAAGAGC TGGTTTCAGA GGGGGCTTCT CTTTGTCCAA AAGTGATCAG TGATGATAAT 
TGGTCTTTAT TATCATCTGA AAAGGGTCCA TCTTTATCTT CAGGGCTTTC ACTGCCAGTT 
CATCCTGATG TGTTAGATGA AAATTGTATG TTTGAAGTAT CTTCTAACAC TGCTTTAGGT 
AAAGATAATG TATACAGCTC AGAAAAGAGT AAGCCCTGCA TCTCTTCCAT ACTCTTAGAA 
GATCTTGCGG TCTCTTTAAC AGTACCGTCA CCTCTGAAAT CAGATGGCCA TTTGAGTTTC 
TTAAAGCCAG AAGTTTTGTC AACTTCAACT CCTGAAGAAG TTATTAGTGC ACATTTTAGT 
GAGGATGCTT TGCTTGAGGA AGAGGATGCA TCTGAACAGG ACATTCATCT AGCTCTGGAG 
TCTGATAACT CAAGCAGTAA GTCAAGCTGT TCATCATGGA CAAGCCGGTC TGTTGCTTCA 
GGCTTTCAGT ACCACCCTAA TCTTCCCATG CATGCTGTCA TAATGGAAAA GTCCAATGAT 
CATTTCATTG TGAAAATACG GCGTGCAACA CCATCTACCT CCCCTGGCCT TAAACATGGT 
GTGGTAGCTG AGGAGTCATT GACATCTTTG CCTAGAACTG GAAAAGAAGC TGGTGTAGCA 
ACAGAGAAAG AACCTAACCT GTTTCAGAGT ACAGTTTTAA AACCTGTCAA GGACTTGGAA 
AATACTGATA AAAATATTGA TAAGAGTAAA CTAACTCATG AAGAACAGAA CTCTATAGTA 
CAAACACAGG TTCCAGATAT ATATGAATTT CTTAAAGATG CCTCAAATAA GGTGGTTCAT 
TGTGATCAAG TGGTTGATGA TTGCTTCAAG TTGCATCAAG TATGGGAACC AAAAGTTTCT 
GAGAACCTTC AAGAGTTGCC TTCAATGGAG AAAATCCCAC ACTCTCTTGA TAATCATCTT 
CCTGATACAC ACATAGATCT AACGAAAGAT TCAGCCACTG AGACCAAAAG CTTGGGGGAG 
CTAATGGAAG TAACAGTTTT AAATGTTGAT CACTTGGAAT GTTCTCAAAC CAACTTAGAT 
CAAGATGCAG AGATAACATG TAGTTCTTTA CAGCCTGATA CTATAGATGC TTTTATTGAT 
TTGACACATG ATGCTTCAAG TGAGAGTAAA AATGAAGGTA GTGAACCTGT GTTAGCTGTT 
GAAGGTATGG GATGCCAGGT AATATGTATA GATGAGGATA CCAACAAAGA AGGAAAGATG 
GGAAGGGCAA ACAGTCCTTT AGAAAGTATT GTTGAAGAAA CTTGTATTGA TTTGACCTCA 
GAGTCTCCTG GCTCCTGTGA AATCAAGAGA CATAATTTAA AGTCGGAGCC TCCATCAAAG 
TTGGATTGTT TAGAGTTGCC TGAAACTCTG GGTAATGGTC ACAAGAAGAG GAAAAACAGT 
CCTGGTGTTA GTCACTCTTC TCAGAAAAAA CAAAGAAAGG ACATAGACTT AAGTAGTGAA 
AAGACCCAGA GACTTAGTCC CAATTCTGAT AGAAATGGTG ATGCTCACAG AAAGCAAGCG 
AGCAAGAAAA GAGAACCTGC AGTAAATGAA ACGTCCTTGT CATCAGAGGC CAGCCCAGAG 
GTGAAGGGTT CAACAGCAGT ACTTGCTGCT TCCCCAGCAA GCCTTTCTGC AAAAAATGTT 
ATCAAAAAGA AGGGAGAAAT TATAGTTTCA TGGACAAGAA ATGATGACCG GGAAATTTTA 
CTGGAATGTC AGAAAAGAAT GCCGTCCCTG AAAACATTTA CTTATTTAGC TGTCAAGCTG 
AATAAAAATC CAAATCAGGT TTCAGAGAGG TTCCAGCAGC TGAAAAAGCT CTTTGAGAAG 
TCAAAATGCA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Maternal expression and early induction of histone gene transcription factor Hinfp sustains development in pre-implantation embryos.
Developmental biology419(2)311-320(2016 11)
Ghule PN,Xie RL,Colby JL,Rivera-P?rez JA,Jones SN,Lian JB,Stein JL,van Wijnen AJ,Stein GS

A Novel Interaction between FLICE-Associated Huge Protein (FLASH) and E2A Regulates Cell Proliferation and Cellular Senescence via Tumor Necrosis Factor (TNF)-Alpha-p21WAF1/CIP1 Axis.
PloS one10(7)e0133205(2015)
Hirano T,Murakami T,Ono H,Sakurai A,Tominaga T,Takahashi T,Nagai K,Doi T,Abe H

FLASH/casp8ap2 is indispensable for early embryogenesis but dispensable for proliferation and differentiation of ES cells.
PloS one9(9)e108032(2014)
Minamida Y,Someda M,Yonehara S

SUMO regulates proteasome-dependent degradation of FLASH/Casp8AP2.
Cell cycle (Georgetown, Tex.)12(12)1914-21(2013 Jun)
Vennemann A,Hofmann TG

FLASH is essential during early embryogenesis and cooperates with p73 to regulate histone gene transcription.
Oncogene31(5)573-82(2012 Feb)
De Cola A,Bongiorno-Borbone L,Bianchi E,Barcaroli D,Carletti E,Knight RA,Di Ilio C,Melino G,Sette C,De Laurenzi V

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Casp8ap2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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