Hunk cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hunk
Entrez Gene ID	26559
Full Name	hormonally upregulated Neu-associated kinase
Synonyms	Bstk1,Mak-v
General protein information	Preferred Names hormonally upregulated Neu-associated kinase Names hormonally up-regulated neu tumor-associated kinase serine/threonine-protein kinase MAK-V
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 16 Map Location: 16 C3.3\|16 51.71 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_015755.2	NP_056570.1	hormonally up-regulated neu tumor-associated kinase
XM_011246130.2	XP_011244432.1	hormonally up-regulated neu tumor-associated kinase isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Hunk	NP_056570.1
Rattus norvegicus (Norway rat)	Hunk	NP_001178591.1
Pan troglodytes (chimpanzee)	HUNK	NP_001065290.1
	LOC709993	XP_002803212.1
Bos taurus (cattle)	HUNK	XP_002684636.1
Xenopus tropicalis (tropical clawed frog)	hunk	NP_001120549.1
Canis lupus familiaris (dog)	HUNK	XP_005638886.1
Gallus gallus (chicken)	HUNK	XP_003640526.2
Homo sapiens (human)	HUNK	NP_055401.1

Gene Ontology
Bibliography

Related articles in PubMed

Hunk/Mak-v is a negative regulator of intestinal cell proliferation.
Reed KR, Korobko IV, Ninkina N, Korobko EV, Hopkins BR, Platt JL, Buchman V, Clarke AR
BMC cancer15110(2015 Mar)

Hunk negatively regulates c-myc to promote Akt-mediated cell survival and mammary tumorigenesis induced by loss of Pten.
Yeh ES, Belka GK, Vernon AE, Chen CC, Jung JJ, Chodosh LA
Proceedings of the National Academy of Sciences of the United States of America110(15)6103-8(2013 Apr)

Genetic analysis of Down syndrome-associated heart defects in mice.
Liu C, Morishima M, Yu T, Matsui S, Zhang L, Fu D, Pao A, Costa AC, Gardiner KJ, Cowell JK, Nowak NJ, Nowak NJ, Parmacek MS, Liang P, Baldini A, Yu YE
Human genetics130(5)623-32(2011 Nov)

Hunk is required for HER2/neu-induced mammary tumorigenesis.
Yeh ES, Yang TW, Jung JJ, Gardner HP, Cardiff RD, Chodosh LA
The Journal of clinical investigation121(3)866-79(2011 Mar)

HUNK suppresses metastasis of basal type breast cancers by disrupting the interaction between PP2A and cofilin-1.
Quintela-Fandino M, Arpaia E, Brenner D, Goh T, Yeung FA, Blaser H, Alexandrova R, Lind EF, Tusche MW, Wakeham A, Ohashi PS, Mak TW
Proceedings of the National Academy of Sciences of the United States of America107(6)2622-7(2010 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Hunk has a role in normal intestinal proliferation and homeostasis and, although it does not alter overall survival rates, activity of this kinase does impact on tumour initiation rates during the early stages in tumourigenesis in the small intestine.
Title: Hunk/Mak-v is a negative regulator of intestinal cell proliferation.

Hunk is a previously unrecognized link between the Akt and c-myc oncogenic pathway induced by loss of Pten
Title: Hunk negatively regulates c-myc to promote Akt-mediated cell survival and mammary tumorigenesis induced by loss of Pten.

Hunk is required for HER2/neu-induced mammary tumorigenesis
Title: Hunk is required for HER2/neu-induced mammary tumorigenesis.

Data show that HUNK reconstitution in basal breast cancer cell lines prevented protein phosphatase 2-A (PP2A from binding to CFL-1.
Title: HUNK suppresses metastasis of basal type breast cancers by disrupting the interaction between PP2A and cofilin-1.

Reconstitution experiments revealed that Hunk is sufficient to restore the metastatic potential of Hunk-deficient mammary tumor cells, as well as defects in migration and invasion, and does so in a manner that requires its kinase activity.
Title: The Snf1-related kinase, Hunk, is essential for mammary tumor metastasis.

The following Hunk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hunk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu69224	XM_011246130.2 Latest version!	Mus musculus hormonally upregulated Neu-associated kinase (Hunk), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu10219	NM_015755.2 Latest version!	Mus musculus hormonally upregulated Neu-associated kinase (Hunk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$419.30 $599.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hunk ( XM_011246130.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244432.1 Mus musculus hormonally upregulated Neu-associated kinase (Hunk), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu69224
Clone ID Related Accession (Same CDS sequence)	XM_011246130.2
Accession Version	XM_011246130.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	hormonally up-regulated neu tumor-associated kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011246130.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCTG TCTGGAGCCA GCACAGGCCT CCCTTAGTCA AGAAGGGACT TCGAGACCCA 
AGGAATGAGG AAATGCTCCT ACAGGTAGCT ATCAAGGTCA TCGATAAGAA AAGAGCCAAG 
AAAGACACCT ACGTCACCAA AAACCTGCGT CGAGAGGGGC AGATCCAGCA GATGATCCGA 
CACCCCAACA TCACACAGCT CCTGGACATC TTGGAGACAG AGAACAGCTA CTACCTGGTC 
ATGGAGCTGT GTCCTGGTGG CAACCTCATG CACAAGATCT ACGAAAAGAA ACGGTTGGAT 
GAAGCCGAGG CCCGCAGATA CATCCGGCAA CTCATCTCTG CGGTGGAACA CCTGCACCGT 
GCGGGGGTGG TTCACAGAGA CTTGAAGATA GAGAATTTGC TACTAGATGA AGACAATAAT 
ATCAAGCTGA TTGACTTTGG CTTGAGCAAC TGTGCAGGGA TCCTAGGTTA CTCGGATCCA 
TTCAGCACAC AGTGTGGCAG CCCTGCCTAT GCTGCGCCAG AACTGCTTGC CAGGAAGAAA 
TATGGCCCCA AAATTGATGT CTGGTCAATA GGCGTGAACA TGTATGCCAT GCTGACGGGG 
ACCCTACCTT TCACTGTGGA GCCTTTCAGC CTGAGGGCTC TGTATCAGAA GATGGTGGAC 
AAAGCAATGA ATCCCCTGCC GACCCAGCTC TCCACAGGGG CCGTCAACTT TCTGCGCTCC 
CTCCTGGAAC CAGACCCTGT GAAGAGGCCG AATATCCAGC AAGCGCTGGC GAATCGCTGG 
TTGAATGAGA ATTACACTGG AAAGGTGCCC TGCAATGTCA CCTATCCCAA CAGGATTTCT 
TTGGAAGACC TGAGTCCCAG CGTGGTGCTG CACATGACTG AAAAGCTGGG CTATAAGAAC 
AGTGACGTCA TCAACACGGT GCTCTCCAAC CGCGCCTGCC ACATCCTGGC CATCTACTTC 
CTGTTGAACA AGAAACTTGA GCGCTATTTG TCAGGGAAAT CAGATATCCA AGATAGCATC 
TGCTACAAGA CCCAGCTCTA CCAGATAGAG AAGTGCAGAG CCACCAAGGA GCCCTATGAG 
GCCTCCCTGG ATACCTGGAC GAGGGACTTT GAATTCCATG CTGTGCAGGA TAAAAAGCCC 
AAAGAACAAG AAAAAAGAGG TGATTTTCTC CACCGTCCGT TTTCCAAGAA GTTGGACAAG 
AATCTGCCTT CTCACAAACA GCCATCGCCC TCGCTGATCA CACAGCTCCA GAGTACCAAA 
GCCCTGCTCA AAGACAGGAA GGCCTCCAAG TCAGGCTTCC CCGACAAAGA TTCCTTCGTC 
TGCCGCAATC TTTTCCGAAA AACCTCTGAT TCCAATTGTG TGGCTTCTTC TTCCATGGAA 
TTCATCCCTG TCCCACCTCC CAGGACACCA AGGATTGTAA AGAAACTAGA GCCACACCAA 
CCAGGGCCGG GAAGTGCCAG CATCCTCCCC AAGGAAGAGC CCCTGCTGCT GGATATGGTA 
CGCTCCTTTG AGTCTGTGGA TCGAGAGGAC CACATAGAAC TGCTGTCCCC TTCTCACCAT 
TATAGGATCC TGAGCTCGCC TGTGAGCCTG GCTCGTAGGA ATTCTAGTGA GAGGACACTC 
TCCCAGGGGC TGCTGTCCGG AAGTACCTCA CCTCTCCAAA CTCCACTGCA TTCCACGCTG 
GTCTCTTTTG CCCACGAAGA AAAGAACAGC CCCCCGAAAG AGGAGGGTGT GTGTTCACCG 
CCTCCCGTTC CCAGTAATGG CCTCCTGCAG CCTCTGGGGA GCCCCAACTG TGTGAAGAGC 
AGGGGACGGT TCCCCATGAT GGGCATCGGA CAGATGCTGA GGAAGCGGCA CCAGAGCCTG 
CAGCCTTCCT CAGAGAGGTC CCTGGACGCC AGCATGTCCC CTCTGCAGCC CACAGCCCCC 
TCCAGCCTCT CCTTTGACAT GGCCGACGGT GTCAAGGGCC AGTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244432.1
CDS	748..2715
Translation	MWAVWSQHRPPLVKKGLRDPRNEEMLLQVAIKVIDKKRAKKDTYVTKNLRREGQIQQMIRHPNITQLLDILETENSYYLVMELCPGGNLMHKIYEKKRLDEAEARRYIRQLISAVEHLHRAGVVHRDLKIENLLLDEDNNIKLIDFGLSNCAGILGYSDPFSTQCGSPAYAAPELLARKKYGPKIDVWSIGVNMYAMLTGTLPFTVEPFSLRALYQKMVDKAMNPLPTQLSTGAVNFLRSLLEPDPVKRPNIQQALANRWLNENYTGKVPCNVTYPNRISLEDLSPSVVLHMTEKLGYKNSDVINTVLSNRACHILAIYFLLNKKLERYLSGKSDIQDSICYKTQLYQIEKCRATKEPYEASLDTWTRDFEFHAVQDKKPKEQEKRGDFLHRPFSKKLDKNLPSHKQPSPSLITQLQSTKALLKDRKASKSGFPDKDSFVCRNLFRKTSDSNCVASSSMEFIPVPPPRTPRIVKKLEPHQPGPGSASILPKEEPLLLDMVRSFESVDREDHIELLSPSHHYRILSSPVSLARRNSSERTLSQGLLSGSTSPLQTPLHSTLVSFAHEEKNSPPKEEGVCSPPPVPSNGLLQPLGSPNCVKSRGRFPMMGIGQMLRKRHQSLQPSSERSLDASMSPLQPTAPSSLSFDMADGVKGQC

Target ORF information:

RefSeq Version	XM_011246130.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus hormonally upregulated Neu-associated kinase (Hunk), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011246130.2

ORF Insert Sequence:

ATGTGGGCTG TCTGGAGCCA GCACAGGCCT CCCTTAGTCA AGAAGGGACT TCGAGACCCA 
AGGAATGAGG AAATGCTCCT ACAGGTAGCT ATCAAGGTCA TCGATAAGAA AAGAGCCAAG 
AAAGACACCT ACGTCACCAA AAACCTGCGT CGAGAGGGGC AGATCCAGCA GATGATCCGA 
CACCCCAACA TCACACAGCT CCTGGACATC TTGGAGACAG AGAACAGCTA CTACCTGGTC 
ATGGAGCTGT GTCCTGGTGG CAACCTCATG CACAAGATCT ACGAAAAGAA ACGGTTGGAT 
GAAGCCGAGG CCCGCAGATA CATCCGGCAA CTCATCTCTG CGGTGGAACA CCTGCACCGT 
GCGGGGGTGG TTCACAGAGA CTTGAAGATA GAGAATTTGC TACTAGATGA AGACAATAAT 
ATCAAGCTGA TTGACTTTGG CTTGAGCAAC TGTGCAGGGA TCCTAGGTTA CTCGGATCCA 
TTCAGCACAC AGTGTGGCAG CCCTGCCTAT GCTGCGCCAG AACTGCTTGC CAGGAAGAAA 
TATGGCCCCA AAATTGATGT CTGGTCAATA GGCGTGAACA TGTATGCCAT GCTGACGGGG 
ACCCTACCTT TCACTGTGGA GCCTTTCAGC CTGAGGGCTC TGTATCAGAA GATGGTGGAC 
AAAGCAATGA ATCCCCTGCC GACCCAGCTC TCCACAGGGG CCGTCAACTT TCTGCGCTCC 
CTCCTGGAAC CAGACCCTGT GAAGAGGCCG AATATCCAGC AAGCGCTGGC GAATCGCTGG 
TTGAATGAGA ATTACACTGG AAAGGTGCCC TGCAATGTCA CCTATCCCAA CAGGATTTCT 
TTGGAAGACC TGAGTCCCAG CGTGGTGCTG CACATGACTG AAAAGCTGGG CTATAAGAAC 
AGTGACGTCA TCAACACGGT GCTCTCCAAC CGCGCCTGCC ACATCCTGGC CATCTACTTC 
CTGTTGAACA AGAAACTTGA GCGCTATTTG TCAGGGAAAT CAGATATCCA AGATAGCATC 
TGCTACAAGA CCCAGCTCTA CCAGATAGAG AAGTGCAGAG CCACCAAGGA GCCCTATGAG 
GCCTCCCTGG ATACCTGGAC GAGGGACTTT GAATTCCATG CTGTGCAGGA TAAAAAGCCC 
AAAGAACAAG AAAAAAGAGG TGATTTTCTC CACCGTCCGT TTTCCAAGAA GTTGGACAAG 
AATCTGCCTT CTCACAAACA GCCATCGCCC TCGCTGATCA CACAGCTCCA GAGTACCAAA 
GCCCTGCTCA AAGACAGGAA GGCCTCCAAG TCAGGCTTCC CCGACAAAGA TTCCTTCGTC 
TGCCGCAATC TTTTCCGAAA AACCTCTGAT TCCAATTGTG TGGCTTCTTC TTCCATGGAA 
TTCATCCCTG TCCCACCTCC CAGGACACCA AGGATTGTAA AGAAACTAGA GCCACACCAA 
CCAGGGCCGG GAAGTGCCAG CATCCTCCCC AAGGAAGAGC CCCTGCTGCT GGATATGGTA 
CGCTCCTTTG AGTCTGTGGA TCGAGAGGAC CACATAGAAC TGCTGTCCCC TTCTCACCAT 
TATAGGATCC TGAGCTCGCC TGTGAGCCTG GCTCGTAGGA ATTCTAGTGA GAGGACACTC 
TCCCAGGGGC TGCTGTCCGG AAGTACCTCA CCTCTCCAAA CTCCACTGCA TTCCACGCTG 
GTCTCTTTTG CCCACGAAGA AAAGAACAGC CCCCCGAAAG AGGAGGGTGT GTGTTCACCG 
CCTCCCGTTC CCAGTAATGG CCTCCTGCAG CCTCTGGGGA GCCCCAACTG TGTGAAGAGC 
AGGGGACGGT TCCCCATGAT GGGCATCGGA CAGATGCTGA GGAAGCGGCA CCAGAGCCTG 
CAGCCTTCCT CAGAGAGGTC CCTGGACGCC AGCATGTCCC CTCTGCAGCC CACAGCCCCC 
TCCAGCCTCT CCTTTGACAT GGCCGACGGT GTCAAGGGCC AGTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hunk ( NM_015755.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_056570.1 Mus musculus hormonally upregulated Neu-associated kinase (Hunk), mRNA.

Price & Availability↑

CloneID	OMu10219
Clone ID Related Accession (Same CDS sequence)	NM_015755.2
Accession Version	NM_015755.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2145bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-21
Organism	Mus musculus(house mouse)
Product	hormonally up-regulated neu tumor-associated kinase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC131339.3 and AC133505.3. On Nov 17, 2006 this sequence version replaced NM_015755.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF055919.2, AK147425.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849383 [ECO:0000348] ##Evidence-Data-END##

ATGCCGGCAG CGGCGGGGGA CGGGCTCTTG GGCGAGCCGG CGGCACCGGG GGGCGATGGA 
GGCGCGGAGG ACACGACCAG GCCGGCGGCG GCCTGCGAGG GAAGTTTCCT GCCCGCCTGG 
GTGAGCGGCG TGTCCCGCGA GCGGCTCCGG GACTTCCAGC ACCACAAGCG CGTGGGCAAC 
TACCTCATCG GCAGCAGGAA GCTGGGAGAG GGCTCCTTCG CCAAGGTGCG CGAGGGGCTG 
CACGTGCTGA CGGGAGAAAA GGTAGCTATC AAGGTCATCG ATAAGAAAAG AGCCAAGAAA 
GACACCTACG TCACCAAAAA CCTGCGTCGA GAGGGGCAGA TCCAGCAGAT GATCCGACAC 
CCCAACATCA CACAGCTCCT GGACATCTTG GAGACAGAGA ACAGCTACTA CCTGGTCATG 
GAGCTGTGTC CTGGTGGCAA CCTCATGCAC AAGATCTACG AAAAGAAACG GTTGGATGAA 
GCCGAGGCCC GCAGATACAT CCGGCAACTC ATCTCTGCGG TGGAACACCT GCACCGTGCG 
GGGGTGGTTC ACAGAGACTT GAAGATAGAG AATTTGCTAC TAGATGAAGA CAATAATATC 
AAGCTGATTG ACTTTGGCTT GAGCAACTGT GCAGGGATCC TAGGTTACTC GGATCCATTC 
AGCACACAGT GTGGCAGCCC TGCCTATGCT GCGCCAGAAC TGCTTGCCAG GAAGAAATAT 
GGCCCCAAAA TTGATGTCTG GTCAATAGGC GTGAACATGT ATGCCATGCT GACGGGGACC 
CTACCTTTCA CTGTGGAGCC TTTCAGCCTG AGGGCTCTGT ATCAGAAGAT GGTGGACAAA 
GCAATGAATC CCCTGCCGAC CCAGCTCTCC ACAGGGGCCG TCAACTTTCT GCGCTCCCTC 
CTGGAACCAG ACCCTGTGAA GAGGCCGAAT ATCCAGCAAG CGCTGGCGAA TCGCTGGTTG 
AATGAGAATT ACACTGGAAA GGTGCCCTGC AATGTCACCT ATCCCAACAG GATTTCTTTG 
GAAGACCTGA GTCCCAGCGT GGTGCTGCAC ATGACTGAAA AGCTGGGCTA TAAGAACAGT 
GACGTCATCA ACACGGTGCT CTCCAACCGC GCCTGCCACA TCCTGGCCAT CTACTTCCTG 
TTGAACAAGA AACTTGAGCG CTATTTGTCA GGGAAATCAG ATATCCAAGA TAGCATCTGC 
TACAAGACCC AGCTCTACCA GATAGAGAAG TGCAGAGCCA CCAAGGAGCC CTATGAGGCC 
TCCCTGGATA CCTGGACGAG GGACTTTGAA TTCCATGCTG TGCAGGATAA AAAGCCCAAA 
GAACAAGAAA AAAGAGGTGA TTTTCTCCAC CGTCCGTTTT CCAAGAAGTT GGACAAGAAT 
CTGCCTTCTC ACAAACAGCC ATCGCCCTCG CTGATCACAC AGCTCCAGAG TACCAAAGCC 
CTGCTCAAAG ACAGGAAGGC CTCCAAGTCA GGCTTCCCCG ACAAAGATTC CTTCGTCTGC 
CGCAATCTTT TCCGAAAAAC CTCTGATTCC AATTGTGTGG CTTCTTCTTC CATGGAATTC 
ATCCCTGTCC CACCTCCCAG GACACCAAGG ATTGTAAAGA AACTAGAGCC ACACCAACCA 
GGGCCGGGAA GTGCCAGCAT CCTCCCCAAG GAAGAGCCCC TGCTGCTGGA TATGGTACGC 
TCCTTTGAGT CTGTGGATCG AGAGGACCAC ATAGAACTGC TGTCCCCTTC TCACCATTAT 
AGGATCCTGA GCTCGCCTGT GAGCCTGGCT CGTAGGAATT CTAGTGAGAG GACACTCTCC 
CAGGGGCTGC TGTCCGGAAG TACCTCACCT CTCCAAACTC CACTGCATTC CACGCTGGTC 
TCTTTTGCCC ACGAAGAAAA GAACAGCCCC CCGAAAGAGG AGGGTGTGTG TTCACCGCCT 
CCCGTTCCCA GTAATGGCCT CCTGCAGCCT CTGGGGAGCC CCAACTGTGT GAAGAGCAGG 
GGACGGTTCC CCATGATGGG CATCGGACAG ATGCTGAGGA AGCGGCACCA GAGCCTGCAG 
CCTTCCTCAG AGAGGTCCCT GGACGCCAGC ATGTCCCCTC TGCAGCCCAC AGCCCCCTCC 
AGCCTCTCCT TTGACATGGC CGACGGTGTC AAGGGCCAGT GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056570.1
CDS	60..2204
Translation	MPAAAGDGLLGEPAAPGGDGGAEDTTRPAAACEGSFLPAWVSGVSRERLRDFQHHKRVGNYLIGSRKLGEGSFAKVREGLHVLTGEKVAIKVIDKKRAKKDTYVTKNLRREGQIQQMIRHPNITQLLDILETENSYYLVMELCPGGNLMHKIYEKKRLDEAEARRYIRQLISAVEHLHRAGVVHRDLKIENLLLDEDNNIKLIDFGLSNCAGILGYSDPFSTQCGSPAYAAPELLARKKYGPKIDVWSIGVNMYAMLTGTLPFTVEPFSLRALYQKMVDKAMNPLPTQLSTGAVNFLRSLLEPDPVKRPNIQQALANRWLNENYTGKVPCNVTYPNRISLEDLSPSVVLHMTEKLGYKNSDVINTVLSNRACHILAIYFLLNKKLERYLSGKSDIQDSICYKTQLYQIEKCRATKEPYEASLDTWTRDFEFHAVQDKKPKEQEKRGDFLHRPFSKKLDKNLPSHKQPSPSLITQLQSTKALLKDRKASKSGFPDKDSFVCRNLFRKTSDSNCVASSSMEFIPVPPPRTPRIVKKLEPHQPGPGSASILPKEEPLLLDMVRSFESVDREDHIELLSPSHHYRILSSPVSLARRNSSERTLSQGLLSGSTSPLQTPLHSTLVSFAHEEKNSPPKEEGVCSPPPVPSNGLLQPLGSPNCVKSRGRFPMMGIGQMLRKRHQSLQPSSERSLDASMSPLQPTAPSSLSFDMADGVKGQC

Target ORF information:

RefSeq Version	NM_015755.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus hormonally upregulated Neu-associated kinase (Hunk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015755.2

ORF Insert Sequence:

ATGCCGGCAG CGGCGGGGGA CGGGCTCTTG GGCGAGCCGG CGGCACCGGG GGGCGATGGA 
GGCGCGGAGG ACACGACCAG GCCGGCGGCG GCCTGCGAGG GAAGTTTCCT GCCCGCCTGG 
GTGAGCGGCG TGTCCCGCGA GCGGCTCCGG GACTTCCAGC ACCACAAGCG CGTGGGCAAC 
TACCTCATCG GCAGCAGGAA GCTGGGAGAG GGCTCCTTCG CCAAGGTGCG CGAGGGGCTG 
CACGTGCTGA CGGGAGAAAA GGTAGCTATC AAGGTCATCG ATAAGAAAAG AGCCAAGAAA 
GACACCTACG TCACCAAAAA CCTGCGTCGA GAGGGGCAGA TCCAGCAGAT GATCCGACAC 
CCCAACATCA CACAGCTCCT GGACATCTTG GAGACAGAGA ACAGCTACTA CCTGGTCATG 
GAGCTGTGTC CTGGTGGCAA CCTCATGCAC AAGATCTACG AAAAGAAACG GTTGGATGAA 
GCCGAGGCCC GCAGATACAT CCGGCAACTC ATCTCTGCGG TGGAACACCT GCACCGTGCG 
GGGGTGGTTC ACAGAGACTT GAAGATAGAG AATTTGCTAC TAGATGAAGA CAATAATATC 
AAGCTGATTG ACTTTGGCTT GAGCAACTGT GCAGGGATCC TAGGTTACTC GGATCCATTC 
AGCACACAGT GTGGCAGCCC TGCCTATGCT GCGCCAGAAC TGCTTGCCAG GAAGAAATAT 
GGCCCCAAAA TTGATGTCTG GTCAATAGGC GTGAACATGT ATGCCATGCT GACGGGGACC 
CTACCTTTCA CTGTGGAGCC TTTCAGCCTG AGGGCTCTGT ATCAGAAGAT GGTGGACAAA 
GCAATGAATC CCCTGCCGAC CCAGCTCTCC ACAGGGGCCG TCAACTTTCT GCGCTCCCTC 
CTGGAACCAG ACCCTGTGAA GAGGCCGAAT ATCCAGCAAG CGCTGGCGAA TCGCTGGTTG 
AATGAGAATT ACACTGGAAA GGTGCCCTGC AATGTCACCT ATCCCAACAG GATTTCTTTG 
GAAGACCTGA GTCCCAGCGT GGTGCTGCAC ATGACTGAAA AGCTGGGCTA TAAGAACAGT 
GACGTCATCA ACACGGTGCT CTCCAACCGC GCCTGCCACA TCCTGGCCAT CTACTTCCTG 
TTGAACAAGA AACTTGAGCG CTATTTGTCA GGGAAATCAG ATATCCAAGA TAGCATCTGC 
TACAAGACCC AGCTCTACCA GATAGAGAAG TGCAGAGCCA CCAAGGAGCC CTATGAGGCC 
TCCCTGGATA CCTGGACGAG GGACTTTGAA TTCCATGCTG TGCAGGATAA AAAGCCCAAA 
GAACAAGAAA AAAGAGGTGA TTTTCTCCAC CGTCCGTTTT CCAAGAAGTT GGACAAGAAT 
CTGCCTTCTC ACAAACAGCC ATCGCCCTCG CTGATCACAC AGCTCCAGAG TACCAAAGCC 
CTGCTCAAAG ACAGGAAGGC CTCCAAGTCA GGCTTCCCCG ACAAAGATTC CTTCGTCTGC 
CGCAATCTTT TCCGAAAAAC CTCTGATTCC AATTGTGTGG CTTCTTCTTC CATGGAATTC 
ATCCCTGTCC CACCTCCCAG GACACCAAGG ATTGTAAAGA AACTAGAGCC ACACCAACCA 
GGGCCGGGAA GTGCCAGCAT CCTCCCCAAG GAAGAGCCCC TGCTGCTGGA TATGGTACGC 
TCCTTTGAGT CTGTGGATCG AGAGGACCAC ATAGAACTGC TGTCCCCTTC TCACCATTAT 
AGGATCCTGA GCTCGCCTGT GAGCCTGGCT CGTAGGAATT CTAGTGAGAG GACACTCTCC 
CAGGGGCTGC TGTCCGGAAG TACCTCACCT CTCCAAACTC CACTGCATTC CACGCTGGTC 
TCTTTTGCCC ACGAAGAAAA GAACAGCCCC CCGAAAGAGG AGGGTGTGTG TTCACCGCCT 
CCCGTTCCCA GTAATGGCCT CCTGCAGCCT CTGGGGAGCC CCAACTGTGT GAAGAGCAGG 
GGACGGTTCC CCATGATGGG CATCGGACAG ATGCTGAGGA AGCGGCACCA GAGCCTGCAG 
CCTTCCTCAG AGAGGTCCCT GGACGCCAGC ATGTCCCCTC TGCAGCCCAC AGCCCCCTCC 
AGCCTCTCCT TTGACATGGC CGACGGTGTC AAGGGCCAGT GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Hunk/Mak-v is a negative regulator of intestinal cell proliferation.
BMC cancer15110(2015 Mar)
Reed KR,Korobko IV,Ninkina N,Korobko EV,Hopkins BR,Platt JL,Buchman V,Clarke AR

Hunk negatively regulates c-myc to promote Akt-mediated cell survival and mammary tumorigenesis induced by loss of Pten.
Proceedings of the National Academy of Sciences of the United States of America110(15)6103-8(2013 Apr)
Yeh ES,Belka GK,Vernon AE,Chen CC,Jung JJ,Chodosh LA

Genetic analysis of Down syndrome-associated heart defects in mice.
Human genetics130(5)623-32(2011 Nov)
Liu C,Morishima M,Yu T,Matsui S,Zhang L,Fu D,Pao A,Costa AC,Gardiner KJ,Cowell JK,Nowak NJ,Nowak NJ,Parmacek MS,Liang P,Baldini A,Yu YE

Hunk is required for HER2/neu-induced mammary tumorigenesis.
The Journal of clinical investigation121(3)866-79(2011 Mar)
Yeh ES,Yang TW,Jung JJ,Gardner HP,Cardiff RD,Chodosh LA

HUNK suppresses metastasis of basal type breast cancers by disrupting the interaction between PP2A and cofilin-1.
Proceedings of the National Academy of Sciences of the United States of America107(6)2622-7(2010 Feb)
Quintela-Fandino M,Arpaia E,Brenner D,Goh T,Yeung FA,Blaser H,Alexandrova R,Lind EF,Tusche MW,Wakeham A,Ohashi PS,Mak TW

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Hunk cDNA ORF clone, Mus musculus(house mouse)

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ORF Insert Sequence:

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