MAP3K4 cDNA ORF clone, Mus musculus(House mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	MAP3K4
Entrez Gene ID	26407
Full Name	mitogen-activated protein kinase kinase kinase 4
Synonyms	D17Rp17,D17Rp17e,MAPKKK4,MEKK 4,MTK1,Mek4b,Mekk4,RP17,Rp17a,Tas,mKIAA0213
General protein information	Preferred Names mitogen-activated protein kinase kinase kinase 4 Names mitogen-activated protein kinase kinase kinase 4 MAPK/ERK kinase kinase 4 MEK kinase 4 T-associated sex reversal
Gene Type	protein-coding
Organism	Mus musculus(House mouse)
Genome	Chromosome: 17 Map Location: 17 8.42 cM\|17 A1

mRNA and Protein(s)

mRNA	Protein	Name
XM_030249819.1	XP_030105679.1	mitogen-activated protein kinase kinase kinase 4 isoform X1
XM_030249820.1	XP_030105680.1	mitogen-activated protein kinase kinase kinase 4 isoform X2
NM_001357722.1	NP_001344651.1	mitogen-activated protein kinase kinase kinase 4 isoform 2
NM_011948.2	NP_036078.2	mitogen-activated protein kinase kinase kinase 4 isoform 1
XM_006523329.3	XP_006523392.1	mitogen-activated protein kinase kinase kinase 4 isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Map3k4	NP_036078.2
Gallus gallus (chicken)	MAP3K4	XP_004935618.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002371	XP_312585.5
	MAP3K4	XP_001098969.2
Canis lupus familiaris (dog)	MAP3K4	XP_005615756.1
Danio rerio (zebrafish)	map3k4	XP_005157074.1
Xenopus tropicalis (tropical clawed frog)	map3k4	XP_004914705.1
Pan troglodytes (chimpanzee)	MAP3K4	XP_003311625.2
Bos taurus (cattle)	MAP3K4	XP_002690423.2
Rattus norvegicus (Norway rat)	Map3k4	NP_001100926.1
Drosophila melanogaster (fruit fly)	Mekk1	NP_650750.4
Homo sapiens (human)	MAP3K4	NP_005913.2

Gene Ontology
Bibliography

Related articles in PubMed

MAP3K4 Controls the Chromatin Modifier HDAC6 during Trophoblast Stem Cell Epithelial-to-Mesenchymal Transition.
Mobley RJ, Raghu D, Duke LD, Abell-Hart K, Zawistowski JS, Lutz K, Gomez SM, Roy S, Homayouni R, Johnson GL, Abell AN
Cell reports18(10)2387-2400(2017 Mar)

Gadd45a Protein Promotes Skeletal Muscle Atrophy by Forming a Complex with the Protein Kinase MEKK4.
Bullard SA, Seo S, Schilling B, Dyle MC, Dierdorff JM, Ebert SM, DeLau AD, Gibson BW, Adams CM
The Journal of biological chemistry291(34)17496-17509(2016 08)

Genetic Analyses Reveal Functions for MAP2K3 and MAP2K6 in Mouse Testis Determination.
Warr N, Siggers P, Carr? GA, Wells S, Greenfield A
Biology of reproduction94(5)103(2016 05)

MEKK4 Signaling Regulates Sensory Cell Development and Function in the Mouse Inner Ear.
Haque K, Pandey AK, Zheng HW, Riazuddin S, Sha SH, Puligilla C
The Journal of neuroscience : the official journal of the Society for Neuroscience36(4)1347-61(2016 Jan)

Transgenic expression of Map3k4 rescues T-associated sex reversal (Tas) in mice.
Warr N, Siggers P, Carr? GA, Bogani D, Brixey R, Akiyoshi M, Tachibana M, Teboul L, Wells S, Sanderson J, Greenfield A
Human molecular genetics23(11)3035-44(2014 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

MAP3K4 activity controls epithelial-to-mesenchymal transition through the ubiquitination and degradation of HDAC6.
Title: MAP3K4 Controls the Chromatin Modifier HDAC6 during Trophoblast Stem Cell Epithelial-to-Mesenchymal Transition.

by forming a complex with MEKK4 in skeletal muscle fibers, Gadd45a increases MEKK4 protein kinase activity, which is both sufficient to induce skeletal muscle fiber atrophy and required for Gadd45a-mediated skeletal muscle fiber atrophy.
Title: Gadd45a Protein Promotes Skeletal Muscle Atrophy by Forming a Complex with the Protein Kinase MEKK4.

Map3k4 haploinsufficiency is the cause of T-associated sex reversal.
Title: Transgenic expression of Map3k4 rescues T-associated sex reversal (Tas) in mice.

suggest a requirement for GADD45gamma in promoting MAP3K4-mediated activation of p38 MAPK signaling in embryonic gonadal somatic cells for testis determination
Title: Gadd45? and Map3k4 interactions regulate mouse testis determination via p38 MAPK-mediated control of Sry expression.

regulates epithelial-mesenchymal transition in trophoblast stem cells
Title: MAP3K4/CBP-regulated H2B acetylation controls epithelial-mesenchymal transition in trophoblast stem cells.

The following MAP3K4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAP3K4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu87206	XM_030249820.1 Latest version!	Mus musculus mitogen-activated protein kinase kinase kinase 4 (Map3k4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu15171	NM_011948.2 Latest version!	Mus musculus mitogen-activated protein kinase kinase kinase 4 (Map3k4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	22	$937.30 $1339.00
OMu87205	XM_030249819.1 Latest version!	Mus musculus mitogen-activated protein kinase kinase kinase 4 (Map3k4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu52902	NM_001357722.1 Latest version!	Mus musculus mitogen-activated protein kinase kinase kinase 4 (Map3k4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu52902	XM_006523329.3 Latest version!	Mus musculus mitogen-activated protein kinase kinase kinase 4 (Map3k4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MAP3K4 ( XM_030249820.1 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_030105680.1 Mus musculus mitogen-activated protein kinase kinase kinase 4 (Map3k4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu87206
Clone ID Related Accession (Same CDS sequence)	XM_030249820.1
Accession Version	XM_030249820.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4467bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	mitogen-activated protein kinase kinase kinase 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACACAC CGAGTCAGTC TCCACATAAA GATTTGGGGA AGGGAGTGGA GACCGTGGAA 
GAATACAGCT ACAAGCAGGA GAAGAAGATT CGAGCAACTC TGAGAACAAC GGAGCGAGAC 
CATAAGAAAA ATGCACAGTG CTCGTTCATG TTGGACTCGG TGGCTGGGTC TTTGCCAAAA 
AAATCGATTC CAGATGTGGA TCTCAATAAG CCTTACCTCA GTCTCGGCTG TAGCAATGCC 
AAGCTGCCCG TCTCGATGCC CATGCCGATA GCCAGAACTG CACGGCAGAC TTCCCGGACT 
GACTGCCCCG CAGATCGCTT AAAGTTCTTT GAAACACTGC GCCTTTTGCT AAAGCTTACC 
TCAGTCTCGA AGAAGAAGGA CAGGGAGCAG AGGGGACAAG AAAACACGGC TGCTTTCTGG 
TTCAACCGAT CGAACGAACT GATCTGGTTA GAACTGCAGG CCTGGCACGC GGGCCGCACC 
ATCAATGACC AGGACCTCTT TCTCTACACA GCCCGCCAGG CCATCCCAGA CATCATCAAT 
GAGATCCTCA CCTTCAAAGT TAACTACGGG AGCATTGCCT TCTCCAGCAA TGGAGCCGGT 
TTCAACGGGC CCTTGGTAGA AGGCCAGTGC AGAACCCCTC AGGAGACAAA CCGTGTGGGC 
TGCTCATCGT ACCACGAGCA CCTCCAGCGC CAGAGGGTCT CGTTTGAGCA GGTGAAGCGG 
ATAATGGAGC TGCTGGAGTA CATGGAGGCA CTTTACCCAT CCTTGCAGGC TCTGCAGAAG 
GACTATGAAC GGTACGCCGC CAAGGACTTT GAGGACAGAG TGCAGGCGCT CTGCCTGTGG 
CTCAACATCA CGAAAGATCT AAATCAGAAG CTGCGGATCA TGGGCACCGT GCTGGGCATC 
AAGAACCTAT CAGACATTGG CTGGCCAGTG TTTGAAATCC CCTCCCCTCG GCCGTCCAAG 
GGCTACGAGC CAGAGGACGA GGTCGAGGAC ACGGAGGTTG AGCTGAGGGA GCTGGAGAGC 
GGGACGGAGG AGAGTGACGA GGAGCCAACC CCCAGTCCGA GGGTGCCAGA GCTCAGGCTG 
TCCACAGACG CCATCTTGGA CAGTCGCTCC CAGGGCTGCG TCTCCAGGAA GCTGGAGAGG 
CTCGAGTCAG AGGAAGATTC CATAGGCTGG GGGACAGCGG ACTGTGGCCC TGAAGCCAGC 
AGGCATTGTT TGACTTCTAT CTATAGACCA TTCGTGGACA AAGCACTGAA GCAAATGGGG 
CTAAGAAAGT TAATTTTACG ACTTCATAAG CTTATGAATG GGTCCTTGCA AAGAGCTCGT 
GTAGCTCTGG TGAAGGACGA CCGTCCAGTG GAGTTCTCTG ACTTTCCAGG TCCCATGTGG 
GGCTCGGATT ATGTGCAGTT GTCGGGAACA CCTCCTTCCT CAGAGCAGAA GTGTAGCGCT 
GTGTCCTGGG AAGAACTGAG AGCCATGGAC CTGCCTTCCT TTGAGCCCGC CTTCCTGGTG 
CTCTGTCGGG TCCTGCTGAA CGTGATCCAC GAGTGCCTGA AGCTGCGGCT GGAACAGAGG 
CCTGCCGGGG AGCCTTCCCT CTTGAGTATC AAACAGCTAG TGCGAGAGTG TAAAGAGGTC 
CTAAAGGGCG GGCTCCTGAT GAAGCAGTAT TACCAGTTCA TGCTGCAGGA GGTCCTGGGC 
GGACTGGAGA AGACCGACTG CAACATGGAT GCCTTTGAGG AGGACCTGCA GAAGATGCTG 
ATGGTGTATT TTGATTACAT GAGAAGCTGG ATCCAAATGC TACAGCAGTT ACCTCAGGCT 
TCCCATAGCT TAAAAAACCT GCTAGAAGAG GAATGGAATT TCACCAAAGA AATAACCCAT 
TATATCCGTG GCGGAGAAGC GCAGGCTGGA AAGCTTTTCT GTGACATCGC AGGGATGCTG 
CTGAAATCCA CAGGGAGCTT TCTGGAATCC GGCCTGCAGG AGAGCTGTGC TGAGCTGTGG 
ACCAGCGCCG ACGACAACGG TGCTGCCGAC GAGCTAAGGA GATCTGTCAT CGAGATCAGC 
CGAGCACTCA AGGAGCTCTT CCACGAAGCC AGGGAAAGAG CCTCCAAGGC CCTGGGCTTT 
GCTAAAATGC TGAGGAAGGA CCTAGAAATA GCAGCAGAGT TCGTGCTATC TGCATCAGCC 
CGAGAGCTCC TGGACGCTCT GAAAGCAAAG CAGTATGTTA AGGTACAGAT TCCCGGGTTA 
GAGAATTTGC ACGTGTTTGT CCCCGACAGC CTCGCTGAGG AGAAGAAAAT TATTTTGCAG 
CTACTCAATG CTGCCACAGG AAAGGACTGC TCAAAGGATC CAGACGACGT CTTCATGGAT 
GCCTTCCTGC TCCTGACCAA GCATGGGGAC CGAGCCCGTG ACTCAGAAGA TGGCTGGGGC 
ACATGGGAAG CTCGGGCTGT CAAAATTGTG CCTCAGGTGG AGACTGTGGA CACCCTGAGA 
AGCATGCAGG TGGACAACCT TCTGCTGGTT GTCATGGAGT CTGCTCACCT CGTACTTCAG 
AGAAAAGCCT TCCAGCAGTC CATTGAGGGG CTGATGACTG TACGCCATGA GCAGACATCT 
AGCCAGCCCA TCATCGCCAA AGGTTTGCAG CAGCTCAAGA ACGATGCACT TGAGCTATGC 
AACAGAATCA GCGATGCCAT CGACCGTGTG GACCACATGT TCACCCTGGA GTTCGATGCT 
GAGGTCGAGG AGTCTGAGTC GGCCACGCTG CAGCAGTACT ACCGAGAAGC CATGATTCAG 
GGCTACAACT TTGGGTTTGA GTATCATAAA GAAGTTGTTC GTTTGATGTC TGGGGAATTC 
AGGCAGAAGA TAGGAGACAA ATATATAAGC TTCGCCCAGA AGTGGATGAA TTACGTGCTG 
ACCAAATGCG AGAGCGGCAG AGGCACAAGA CCCAGATGGG CCACCCAAGG ATTTGATTTC 
CTACAAGCCA TTGAACCTGC CTTTATTTCA GCTTTACCAG AAGATGACTT CTTGAGTTTG 
CAAGCCCTGA TGAATGAGTG CATCGGGCAC GTCATAGGAA AGCCACACAG CCCTGTCACA 
GCTATCCATC GGAACAGCCC CCGCCCTGTG AAGGTGCCCC GATGCCACAG TGACCCTCCT 
AACCCTCACC TCATCATCCC GACTCCAGAG GGATTCAGCA CCCGGAGCGT GCCTTCCGAC 
GCTCGGACCC ATGGCAACTC TGTTGCTGCT GCTGCTGCTG TTGCTGCCGC CGCCACCACT 
GCTGCTGGCC GCCCTGGCCC AGGTGGTGGT GACTCTGTGC CAGCCAAACC TGTCAACACT 
GCCCCTGATA CCAGGGGTTC CAGTGTCCCT GAAAACGACC GCTTGGCCTC CATAGCTGCA 
GAACTGCAGT TCAGGTCTCT GAGTCGGCAC TCAAGCCCCA CGGAAGAGCG AGACGAGCCA 
GCGTATCCTC GGAGTGACTC AAGTGGATCA ACTCGGAGAA GCTGGGAACT TCGAACACTC 
ATCAGCCAGA CCAAAGACTC GGCCTCTAAG CAGGGGCCCA TAGAAGCTAT CCAGAAGTCA 
GTCCGACTGT TTGAAGAGAG GAGGTATCGA GAGATGAGGA GAAAGAATAT CATCGGCCAA 
GTGTGCGATA CCCCTAAGTC CTATGATAAC GTCATGCATG TTGGACTGAG GAAGGTGACA 
TTTAAGTGGC AAAGAGGAAA CAAAATTGGA GAAGGACAGT ATGGAAAAGT ATACACCTGC 
ATCAGTGTTG ACACAGGGGA GCTGATGGCC ATGAAGGAGA TTCGATTTCA GCCTAACGAC 
CACAAGACTA TCAAGGAGAC TGCAGACGAG TTGAAAATAT TTGAAGGCAT CAAGCACCCC 
AACCTGGTCC GGTATTTTGG CGTGGAGCTT CACAGGGAAG AGATGTACAT CTTCATGGAG 
TACTGTGATG AGGGTACACT AGAGGAGGTG TCACGACTGG GCCTGCAGGA GCACGTCATC 
AGGTTATATA CCAAGCAGAT CACTGTCGCC ATCAACGTCC TCCATGAGCA CGGCATCGTT 
CACCGAGACA TCAAAGGTGC CAATATCTTC CTTACGTCAT CTGGACTAAT CAAGCTGGGA 
GATTTTGGAT GCTCTGTAAA ACTTAAAAAC AACGCCCAGA CCATGCCCGG AGAGGTGAAC 
AGCACCCTAG GGACAGCAGC TTACATGGCC CCTGAAGTTA TTACCCGAGC CAAAGGAGAA 
GGCCACGGAC GTGCGGCAGA TATCTGGAGT CTGGGGTGCG TCGTCATAGA GATGGTGACT 
GGCAAGCGGC CTTGGCATGA GTATGAACAC AACTTTCAGA TTATGTACAA GGTGGGGATG 
GGACACAAGC CACCAATCCC GGAAAGGCTA AGCCCTGAAG GAAAGGCCTT TCTCTCGCAC 
TGCCTGGAAA GTGACCCGAA GATACGGTGG ACAGCCAGCC AGCTCCTCGA CCACGCTTTT 
GTCAAGGTTT GCACAGATGA AGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105680.1
CDS	74..4540
Translation	MNTPSQSPHKDLGKGVETVEEYSYKQEKKIRATLRTTERDHKKNAQCSFMLDSVAGSLPKKSIPDVDLNKPYLSLGCSNAKLPVSMPMPIARTARQTSRTDCPADRLKFFETLRLLLKLTSVSKKKDREQRGQENTAAFWFNRSNELIWLELQAWHAGRTINDQDLFLYTARQAIPDIINEILTFKVNYGSIAFSSNGAGFNGPLVEGQCRTPQETNRVGCSSYHEHLQRQRVSFEQVKRIMELLEYMEALYPSLQALQKDYERYAAKDFEDRVQALCLWLNITKDLNQKLRIMGTVLGIKNLSDIGWPVFEIPSPRPSKGYEPEDEVEDTEVELRELESGTEESDEEPTPSPRVPELRLSTDAILDSRSQGCVSRKLERLESEEDSIGWGTADCGPEASRHCLTSIYRPFVDKALKQMGLRKLILRLHKLMNGSLQRARVALVKDDRPVEFSDFPGPMWGSDYVQLSGTPPSSEQKCSAVSWEELRAMDLPSFEPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLQEVLGGLEKTDCNMDAFEEDLQKMLMVYFDYMRSWIQMLQQLPQASHSLKNLLEEEWNFTKEITHYIRGGEAQAGKLFCDIAGMLLKSTGSFLESGLQESCAELWTSADDNGAADELRRSVIEISRALKELFHEARERASKALGFAKMLRKDLEIAAEFVLSASARELLDALKAKQYVKVQIPGLENLHVFVPDSLAEEKKIILQLLNAATGKDCSKDPDDVFMDAFLLLTKHGDRARDSEDGWGTWEARAVKIVPQVETVDTLRSMQVDNLLLVVMESAHLVLQRKAFQQSIEGLMTVRHEQTSSQPIIAKGLQQLKNDALELCNRISDAIDRVDHMFTLEFDAEVEESESATLQQYYREAMIQGYNFGFEYHKEVVRLMSGEFRQKIGDKYISFAQKWMNYVLTKCESGRGTRPRWATQGFDFLQAIEPAFISALPEDDFLSLQALMNECIGHVIGKPHSPVTAIHRNSPRPVKVPRCHSDPPNPHLIIPTPEGFSTRSVPSDARTHGNSVAAAAAVAAAATTAAGRPGPGGGDSVPAKPVNTAPDTRGSSVPENDRLASIAAELQFRSLSRHSSPTEERDEPAYPRSDSSGSTRRSWELRTLISQTKDSASKQGPIEAIQKSVRLFEERRYREMRRKNIIGQVCDTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCISVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSRLGLQEHVIRLYTKQITVAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLKNNAQTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVVIEMVTGKRPWHEYEHNFQIMYKVGMGHKPPIPERLSPEGKAFLSHCLESDPKIRWTASQLLDHAFVKVCTDEE

Target ORF information:

RefSeq Version	XM_030249820.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mitogen-activated protein kinase kinase kinase 4 (Map3k4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249820.1

ORF Insert Sequence:

ATGAACACAC CGAGTCAGTC TCCACATAAA GATTTGGGGA AGGGAGTGGA GACCGTGGAA 
GAATACAGCT ACAAGCAGGA GAAGAAGATT CGAGCAACTC TGAGAACAAC GGAGCGAGAC 
CATAAGAAAA ATGCACAGTG CTCGTTCATG TTGGACTCGG TGGCTGGGTC TTTGCCAAAA 
AAATCGATTC CAGATGTGGA TCTCAATAAG CCTTACCTCA GTCTCGGCTG TAGCAATGCC 
AAGCTGCCCG TCTCGATGCC CATGCCGATA GCCAGAACTG CACGGCAGAC TTCCCGGACT 
GACTGCCCCG CAGATCGCTT AAAGTTCTTT GAAACACTGC GCCTTTTGCT AAAGCTTACC 
TCAGTCTCGA AGAAGAAGGA CAGGGAGCAG AGGGGACAAG AAAACACGGC TGCTTTCTGG 
TTCAACCGAT CGAACGAACT GATCTGGTTA GAACTGCAGG CCTGGCACGC GGGCCGCACC 
ATCAATGACC AGGACCTCTT TCTCTACACA GCCCGCCAGG CCATCCCAGA CATCATCAAT 
GAGATCCTCA CCTTCAAAGT TAACTACGGG AGCATTGCCT TCTCCAGCAA TGGAGCCGGT 
TTCAACGGGC CCTTGGTAGA AGGCCAGTGC AGAACCCCTC AGGAGACAAA CCGTGTGGGC 
TGCTCATCGT ACCACGAGCA CCTCCAGCGC CAGAGGGTCT CGTTTGAGCA GGTGAAGCGG 
ATAATGGAGC TGCTGGAGTA CATGGAGGCA CTTTACCCAT CCTTGCAGGC TCTGCAGAAG 
GACTATGAAC GGTACGCCGC CAAGGACTTT GAGGACAGAG TGCAGGCGCT CTGCCTGTGG 
CTCAACATCA CGAAAGATCT AAATCAGAAG CTGCGGATCA TGGGCACCGT GCTGGGCATC 
AAGAACCTAT CAGACATTGG CTGGCCAGTG TTTGAAATCC CCTCCCCTCG GCCGTCCAAG 
GGCTACGAGC CAGAGGACGA GGTCGAGGAC ACGGAGGTTG AGCTGAGGGA GCTGGAGAGC 
GGGACGGAGG AGAGTGACGA GGAGCCAACC CCCAGTCCGA GGGTGCCAGA GCTCAGGCTG 
TCCACAGACG CCATCTTGGA CAGTCGCTCC CAGGGCTGCG TCTCCAGGAA GCTGGAGAGG 
CTCGAGTCAG AGGAAGATTC CATAGGCTGG GGGACAGCGG ACTGTGGCCC TGAAGCCAGC 
AGGCATTGTT TGACTTCTAT CTATAGACCA TTCGTGGACA AAGCACTGAA GCAAATGGGG 
CTAAGAAAGT TAATTTTACG ACTTCATAAG CTTATGAATG GGTCCTTGCA AAGAGCTCGT 
GTAGCTCTGG TGAAGGACGA CCGTCCAGTG GAGTTCTCTG ACTTTCCAGG TCCCATGTGG 
GGCTCGGATT ATGTGCAGTT GTCGGGAACA CCTCCTTCCT CAGAGCAGAA GTGTAGCGCT 
GTGTCCTGGG AAGAACTGAG AGCCATGGAC CTGCCTTCCT TTGAGCCCGC CTTCCTGGTG 
CTCTGTCGGG TCCTGCTGAA CGTGATCCAC GAGTGCCTGA AGCTGCGGCT GGAACAGAGG 
CCTGCCGGGG AGCCTTCCCT CTTGAGTATC AAACAGCTAG TGCGAGAGTG TAAAGAGGTC 
CTAAAGGGCG GGCTCCTGAT GAAGCAGTAT TACCAGTTCA TGCTGCAGGA GGTCCTGGGC 
GGACTGGAGA AGACCGACTG CAACATGGAT GCCTTTGAGG AGGACCTGCA GAAGATGCTG 
ATGGTGTATT TTGATTACAT GAGAAGCTGG ATCCAAATGC TACAGCAGTT ACCTCAGGCT 
TCCCATAGCT TAAAAAACCT GCTAGAAGAG GAATGGAATT TCACCAAAGA AATAACCCAT 
TATATCCGTG GCGGAGAAGC GCAGGCTGGA AAGCTTTTCT GTGACATCGC AGGGATGCTG 
CTGAAATCCA CAGGGAGCTT TCTGGAATCC GGCCTGCAGG AGAGCTGTGC TGAGCTGTGG 
ACCAGCGCCG ACGACAACGG TGCTGCCGAC GAGCTAAGGA GATCTGTCAT CGAGATCAGC 
CGAGCACTCA AGGAGCTCTT CCACGAAGCC AGGGAAAGAG CCTCCAAGGC CCTGGGCTTT 
GCTAAAATGC TGAGGAAGGA CCTAGAAATA GCAGCAGAGT TCGTGCTATC TGCATCAGCC 
CGAGAGCTCC TGGACGCTCT GAAAGCAAAG CAGTATGTTA AGGTACAGAT TCCCGGGTTA 
GAGAATTTGC ACGTGTTTGT CCCCGACAGC CTCGCTGAGG AGAAGAAAAT TATTTTGCAG 
CTACTCAATG CTGCCACAGG AAAGGACTGC TCAAAGGATC CAGACGACGT CTTCATGGAT 
GCCTTCCTGC TCCTGACCAA GCATGGGGAC CGAGCCCGTG ACTCAGAAGA TGGCTGGGGC 
ACATGGGAAG CTCGGGCTGT CAAAATTGTG CCTCAGGTGG AGACTGTGGA CACCCTGAGA 
AGCATGCAGG TGGACAACCT TCTGCTGGTT GTCATGGAGT CTGCTCACCT CGTACTTCAG 
AGAAAAGCCT TCCAGCAGTC CATTGAGGGG CTGATGACTG TACGCCATGA GCAGACATCT 
AGCCAGCCCA TCATCGCCAA AGGTTTGCAG CAGCTCAAGA ACGATGCACT TGAGCTATGC 
AACAGAATCA GCGATGCCAT CGACCGTGTG GACCACATGT TCACCCTGGA GTTCGATGCT 
GAGGTCGAGG AGTCTGAGTC GGCCACGCTG CAGCAGTACT ACCGAGAAGC CATGATTCAG 
GGCTACAACT TTGGGTTTGA GTATCATAAA GAAGTTGTTC GTTTGATGTC TGGGGAATTC 
AGGCAGAAGA TAGGAGACAA ATATATAAGC TTCGCCCAGA AGTGGATGAA TTACGTGCTG 
ACCAAATGCG AGAGCGGCAG AGGCACAAGA CCCAGATGGG CCACCCAAGG ATTTGATTTC 
CTACAAGCCA TTGAACCTGC CTTTATTTCA GCTTTACCAG AAGATGACTT CTTGAGTTTG 
CAAGCCCTGA TGAATGAGTG CATCGGGCAC GTCATAGGAA AGCCACACAG CCCTGTCACA 
GCTATCCATC GGAACAGCCC CCGCCCTGTG AAGGTGCCCC GATGCCACAG TGACCCTCCT 
AACCCTCACC TCATCATCCC GACTCCAGAG GGATTCAGCA CCCGGAGCGT GCCTTCCGAC 
GCTCGGACCC ATGGCAACTC TGTTGCTGCT GCTGCTGCTG TTGCTGCCGC CGCCACCACT 
GCTGCTGGCC GCCCTGGCCC AGGTGGTGGT GACTCTGTGC CAGCCAAACC TGTCAACACT 
GCCCCTGATA CCAGGGGTTC CAGTGTCCCT GAAAACGACC GCTTGGCCTC CATAGCTGCA 
GAACTGCAGT TCAGGTCTCT GAGTCGGCAC TCAAGCCCCA CGGAAGAGCG AGACGAGCCA 
GCGTATCCTC GGAGTGACTC AAGTGGATCA ACTCGGAGAA GCTGGGAACT TCGAACACTC 
ATCAGCCAGA CCAAAGACTC GGCCTCTAAG CAGGGGCCCA TAGAAGCTAT CCAGAAGTCA 
GTCCGACTGT TTGAAGAGAG GAGGTATCGA GAGATGAGGA GAAAGAATAT CATCGGCCAA 
GTGTGCGATA CCCCTAAGTC CTATGATAAC GTCATGCATG TTGGACTGAG GAAGGTGACA 
TTTAAGTGGC AAAGAGGAAA CAAAATTGGA GAAGGACAGT ATGGAAAAGT ATACACCTGC 
ATCAGTGTTG ACACAGGGGA GCTGATGGCC ATGAAGGAGA TTCGATTTCA GCCTAACGAC 
CACAAGACTA TCAAGGAGAC TGCAGACGAG TTGAAAATAT TTGAAGGCAT CAAGCACCCC 
AACCTGGTCC GGTATTTTGG CGTGGAGCTT CACAGGGAAG AGATGTACAT CTTCATGGAG 
TACTGTGATG AGGGTACACT AGAGGAGGTG TCACGACTGG GCCTGCAGGA GCACGTCATC 
AGGTTATATA CCAAGCAGAT CACTGTCGCC ATCAACGTCC TCCATGAGCA CGGCATCGTT 
CACCGAGACA TCAAAGGTGC CAATATCTTC CTTACGTCAT CTGGACTAAT CAAGCTGGGA 
GATTTTGGAT GCTCTGTAAA ACTTAAAAAC AACGCCCAGA CCATGCCCGG AGAGGTGAAC 
AGCACCCTAG GGACAGCAGC TTACATGGCC CCTGAAGTTA TTACCCGAGC CAAAGGAGAA 
GGCCACGGAC GTGCGGCAGA TATCTGGAGT CTGGGGTGCG TCGTCATAGA GATGGTGACT 
GGCAAGCGGC CTTGGCATGA GTATGAACAC AACTTTCAGA TTATGTACAA GGTGGGGATG 
GGACACAAGC CACCAATCCC GGAAAGGCTA AGCCCTGAAG GAAAGGCCTT TCTCTCGCAC 
TGCCTGGAAA GTGACCCGAA GATACGGTGG ACAGCCAGCC AGCTCCTCGA CCACGCTTTT 
GTCAAGGTTT GCACAGATGA AGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K4 ( NM_011948.2 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_036078.2 Mus musculus mitogen-activated protein kinase kinase kinase 4 (Map3k4), transcript variant 1, mRNA.

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CloneID	OMu15171
Clone ID Related Accession (Same CDS sequence)	NM_011948.2
Accession Version	NM_011948.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4794bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	mitogen-activated protein kinase kinase kinase 4 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC058719.1 and CK634503.1. On Apr 21, 2006 this sequence version replaced NM_011948.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U85607.1, BC058719.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGAGAGACG CCATCGCCGA GCCGGTGCCC CCTCCTGCCC TCGCCGACAC CCCTGCAGCC 
GCCATGGAGG AGCTGCGGCC AGCACCGCCG CCACAGCCCG AGCCGGATCC GGAGTGCTGC 
CCAGCGGCGA GGCAGGAGTG CATGTTGGGA GAGTCGGCTC GCAAAAGTAT GGAATCCGAT 
CCAGAGGACT TTTCTGATGA AACAAATACA GAGACTCTCT ACGGCACCTC ACCCCCAAGC 
ACACCTCGAC AGATGAAACG CCTGTCAGCC AAGCACCAGA GGAACAGCGC AGGGAGGCCG 
GCCAGCCGAT CGAACTTGAA AGAAAAAATG AACACACCGA GTCAGTCTCC ACATAAAGAT 
TTGGGGAAGG GAGTGGAGAC CGTGGAAGAA TACAGCTACA AGCAGGAGAA GAAGATTCGA 
GCAACTCTGA GAACAACGGA GCGAGACCAT AAGAAAAATG CACAGTGCTC GTTCATGTTG 
GACTCGGTGG CTGGGTCTTT GCCAAAAAAA TCGATTCCAG ATGTGGATCT CAATAAGCCT 
TACCTCAGTC TCGGCTGTAG CAATGCCAAG CTGCCCGTCT CGATGCCCAT GCCGATAGCC 
AGAACTGCAC GGCAGACTTC CCGGACTGAC TGCCCCGCAG ATCGCTTAAA GTTCTTTGAA 
ACACTGCGCC TTTTGCTAAA GCTTACCTCA GTCTCGAAGA AGAAGGACAG GGAGCAGAGG 
GGACAAGAAA ACACGGCTGC TTTCTGGTTC AACCGATCGA ACGAACTGAT CTGGTTAGAA 
CTGCAGGCCT GGCACGCGGG CCGCACCATC AATGACCAGG ACCTCTTTCT CTACACAGCC 
CGCCAGGCCA TCCCAGACAT CATCAATGAG ATCCTCACCT TCAAAGTTAA CTACGGGAGC 
ATTGCCTTCT CCAGCAATGG AGCCGGTTTC AACGGGCCCT TGGTAGAAGG CCAGTGCAGA 
ACCCCTCAGG AGACAAACCG TGTGGGCTGC TCATCGTACC ACGAGCACCT CCAGCGCCAG 
AGGGTCTCGT TTGAGCAGGT GAAGCGGATA ATGGAGCTGC TGGAGTACAT GGAGGCACTT 
TACCCATCCT TGCAGGCTCT GCAGAAGGAC TATGAACGGT ACGCCGCCAA GGACTTTGAG 
GACAGAGTGC AGGCGCTCTG CCTGTGGCTC AACATCACGA AAGATCTAAA TCAGAAGCTG 
CGGATCATGG GCACCGTGCT GGGCATCAAG AACCTATCAG ACATTGGCTG GCCAGTGTTT 
GAAATCCCCT CCCCTCGGCC GTCCAAGGGC TACGAGCCAG AGGACGAGGT CGAGGACACG 
GAGGTTGAGC TGAGGGAGCT GGAGAGCGGG ACGGAGGAGA GTGACGAGGA GCCAACCCCC 
AGTCCGAGGG TGCCAGAGCT CAGGCTGTCC ACAGACGCCA TCTTGGACAG TCGCTCCCAG 
GGCTGCGTCT CCAGGAAGCT GGAGAGGCTC GAGTCAGAGG AAGATTCCAT AGGCTGGGGG 
ACAGCGGACT GTGGCCCTGA AGCCAGCAGG CATTGTTTGA CTTCTATCTA TAGACCATTC 
GTGGACAAAG CACTGAAGCA AATGGGGCTA AGAAAGTTAA TTTTACGACT TCATAAGCTT 
ATGAATGGGT CCTTGCAAAG AGCTCGTGTA GCTCTGGTGA AGGACGACCG TCCAGTGGAG 
TTCTCTGACT TTCCAGGTCC CATGTGGGGC TCGGATTATG TGCAGTTGTC GGGAACACCT 
CCTTCCTCAG AGCAGAAGTG TAGCGCTGTG TCCTGGGAAG AACTGAGAGC CATGGACCTG 
CCTTCCTTTG AGCCCGCCTT CCTGGTGCTC TGTCGGGTCC TGCTGAACGT GATCCACGAG 
TGCCTGAAGC TGCGGCTGGA ACAGAGGCCT GCCGGGGAGC CTTCCCTCTT GAGTATCAAA 
CAGCTAGTGC GAGAGTGTAA AGAGGTCCTA AAGGGCGGGC TCCTGATGAA GCAGTATTAC 
CAGTTCATGC TGCAGGAGGT CCTGGGCGGA CTGGAGAAGA CCGACTGCAA CATGGATGCC 
TTTGAGGAGG ACCTGCAGAA GATGCTGATG GTGTATTTTG ATTACATGAG AAGCTGGATC 
CAAATGCTAC AGCAGTTACC TCAGGCTTCC CATAGCTTAA AAAACCTGCT AGAAGAGGAA 
TGGAATTTCA CCAAAGAAAT AACCCATTAT ATCCGTGGCG GAGAAGCGCA GGCTGGAAAG 
CTTTTCTGTG ACATCGCAGG GATGCTGCTG AAATCCACAG GGAGCTTTCT GGAATCCGGC 
CTGCAGGAGA GCTGTGCTGA GCTGTGGACC AGCGCCGACG ACAACGGTGC TGCCGACGAG 
CTAAGGAGAT CTGTCATCGA GATCAGCCGA GCACTCAAGG AGCTCTTCCA CGAAGCCAGG 
GAAAGAGCCT CCAAGGCCCT GGGCTTTGCT AAAATGCTGA GGAAGGACCT AGAAATAGCA 
GCAGAGTTCG TGCTATCTGC ATCAGCCCGA GAGCTCCTGG ACGCTCTGAA AGCAAAGCAG 
TATGTTAAGG TACAGATTCC CGGGTTAGAG AATTTGCACG TGTTTGTCCC CGACAGCCTC 
GCTGAGGAGA AGAAAATTAT TTTGCAGCTA CTCAATGCTG CCACAGGAAA GGACTGCTCA 
AAGGATCCAG ACGACGTCTT CATGGATGCC TTCCTGCTCC TGACCAAGCA TGGGGACCGA 
GCCCGTGACT CAGAAGATGG CTGGGGCACA TGGGAAGCTC GGGCTGTCAA AATTGTGCCT 
CAGGTGGAGA CTGTGGACAC CCTGAGAAGC ATGCAGGTGG ACAACCTTCT GCTGGTTGTC 
ATGGAGTCTG CTCACCTCGT ACTTCAGAGA AAAGCCTTCC AGCAGTCCAT TGAGGGGCTG 
ATGACTGTAC GCCATGAGCA GACATCTAGC CAGCCCATCA TCGCCAAAGG TTTGCAGCAG 
CTCAAGAACG ATGCACTTGA GCTATGCAAC AGAATCAGCG ATGCCATCGA CCGTGTGGAC 
CACATGTTCA CCCTGGAGTT CGATGCTGAG GTCGAGGAGT CTGAGTCGGC CACGCTGCAG 
CAGTACTACC GAGAAGCCAT GATTCAGGGC TACAACTTTG GGTTTGAGTA TCATAAAGAA 
GTTGTTCGTT TGATGTCTGG GGAATTCAGG CAGAAGATAG GAGACAAATA TATAAGCTTC 
GCCCAGAAGT GGATGAATTA CGTGCTGACC AAATGCGAGA GCGGCAGAGG CACAAGACCC 
AGATGGGCCA CCCAAGGATT TGATTTCCTA CAAGCCATTG AACCTGCCTT TATTTCAGCT 
TTACCAGAAG ATGACTTCTT GAGTTTGCAA GCCCTGATGA ATGAGTGCAT CGGGCACGTC 
ATAGGAAAGC CACACAGCCC TGTCACAGCT ATCCATCGGA ACAGCCCCCG CCCTGTGAAG 
GTGCCCCGAT GCCACAGTGA CCCTCCTAAC CCTCACCTCA TCATCCCGAC TCCAGAGGGA 
TTCAGCACCC GGAGCGTGCC TTCCGACGCT CGGACCCATG GCAACTCTGT TGCTGCTGCT 
GCTGCTGTTG CTGCCGCCGC CACCACTGCT GCTGGCCGCC CTGGCCCAGG TGGTGGTGAC 
TCTGTGCCAG CCAAACCTGT CAACACTGCC CCTGATACCA GGGGTTCCAG TGTCCCTGAA 
AACGACCGCT TGGCCTCCAT AGCTGCAGAA CTGCAGTTCA GGTCTCTGAG TCGGCACTCA 
AGCCCCACGG AAGAGCGAGA CGAGCCAGCG TATCCTCGGA GTGACTCAAG TGGATCAACT 
CGGAGAAGCT GGGAACTTCG AACACTCATC AGCCAGACCA AAGACTCGGC CTCTAAGCAG 
GGGCCCATAG AAGCTATCCA GAAGTCAGTC CGACTGTTTG AAGAGAGGAG GTATCGAGAG 
ATGAGGAGAA AGAATATCAT CGGCCAAGTG TGCGATACCC CTAAGTCCTA TGATAACGTC 
ATGCATGTTG GACTGAGGAA GGTGACATTT AAGTGGCAAA GAGGAAACAA AATTGGAGAA 
GGACAGTATG GAAAAGTATA CACCTGCATC AGTGTTGACA CAGGGGAGCT GATGGCCATG 
AAGGAGATTC GATTTCAGCC TAACGACCAC AAGACTATCA AGGAGACTGC AGACGAGTTG 
AAAATATTTG AAGGCATCAA GCACCCCAAC CTGGTCCGGT ATTTTGGCGT GGAGCTTCAC 
AGGGAAGAGA TGTACATCTT CATGGAGTAC TGTGATGAGG GTACACTAGA GGAGGTGTCA 
CGACTGGGCC TGCAGGAGCA CGTCATCAGG TTATATACCA AGCAGATCAC TGTCGCCATC 
AACGTCCTCC ATGAGCACGG CATCGTTCAC CGAGACATCA AAGGTGCCAA TATCTTCCTT 
ACGTCATCTG GACTAATCAA GCTGGGAGAT TTTGGATGCT CTGTAAAACT TAAAAACAAC 
GCCCAGACCA TGCCCGGAGA GGTGAACAGC ACCCTAGGGA CAGCAGCTTA CATGGCCCCT 
GAAGTTATTA CCCGAGCCAA AGGAGAAGGC CACGGACGTG CGGCAGATAT CTGGAGTCTG 
GGGTGCGTCG TCATAGAGAT GGTGACTGGC AAGCGGCCTT GGCATGAGTA TGAACACAAC 
TTTCAGATTA TGTACAAGGT GGGGATGGGA CACAAGCCAC CAATCCCGGA AAGGCTAAGC 
CCTGAAGGAA AGGCCTTTCT CTCGCACTGC CTGGAAAGTG ACCCGAAGAT ACGGTGGACA 
GCCAGCCAGC TCCTCGACCA CGCTTTTGTC AAGGTTTGCA CAGATGAAGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036078.2
CDS	33..4826
Translation	MRDAIAEPVPPPALADTPAAAMEELRPAPPPQPEPDPECCPAARQECMLGESARKSMESDPEDFSDETNTETLYGTSPPSTPRQMKRLSAKHQRNSAGRPASRSNLKEKMNTPSQSPHKDLGKGVETVEEYSYKQEKKIRATLRTTERDHKKNAQCSFMLDSVAGSLPKKSIPDVDLNKPYLSLGCSNAKLPVSMPMPIARTARQTSRTDCPADRLKFFETLRLLLKLTSVSKKKDREQRGQENTAAFWFNRSNELIWLELQAWHAGRTINDQDLFLYTARQAIPDIINEILTFKVNYGSIAFSSNGAGFNGPLVEGQCRTPQETNRVGCSSYHEHLQRQRVSFEQVKRIMELLEYMEALYPSLQALQKDYERYAAKDFEDRVQALCLWLNITKDLNQKLRIMGTVLGIKNLSDIGWPVFEIPSPRPSKGYEPEDEVEDTEVELRELESGTEESDEEPTPSPRVPELRLSTDAILDSRSQGCVSRKLERLESEEDSIGWGTADCGPEASRHCLTSIYRPFVDKALKQMGLRKLILRLHKLMNGSLQRARVALVKDDRPVEFSDFPGPMWGSDYVQLSGTPPSSEQKCSAVSWEELRAMDLPSFEPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLQEVLGGLEKTDCNMDAFEEDLQKMLMVYFDYMRSWIQMLQQLPQASHSLKNLLEEEWNFTKEITHYIRGGEAQAGKLFCDIAGMLLKSTGSFLESGLQESCAELWTSADDNGAADELRRSVIEISRALKELFHEARERASKALGFAKMLRKDLEIAAEFVLSASARELLDALKAKQYVKVQIPGLENLHVFVPDSLAEEKKIILQLLNAATGKDCSKDPDDVFMDAFLLLTKHGDRARDSEDGWGTWEARAVKIVPQVETVDTLRSMQVDNLLLVVMESAHLVLQRKAFQQSIEGLMTVRHEQTSSQPIIAKGLQQLKNDALELCNRISDAIDRVDHMFTLEFDAEVEESESATLQQYYREAMIQGYNFGFEYHKEVVRLMSGEFRQKIGDKYISFAQKWMNYVLTKCESGRGTRPRWATQGFDFLQAIEPAFISALPEDDFLSLQALMNECIGHVIGKPHSPVTAIHRNSPRPVKVPRCHSDPPNPHLIIPTPEGFSTRSVPSDARTHGNSVAAAAAVAAAATTAAGRPGPGGGDSVPAKPVNTAPDTRGSSVPENDRLASIAAELQFRSLSRHSSPTEERDEPAYPRSDSSGSTRRSWELRTLISQTKDSASKQGPIEAIQKSVRLFEERRYREMRRKNIIGQVCDTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCISVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSRLGLQEHVIRLYTKQITVAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLKNNAQTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVVIEMVTGKRPWHEYEHNFQIMYKVGMGHKPPIPERLSPEGKAFLSHCLESDPKIRWTASQLLDHAFVKVCTDEE

Target ORF information:

RefSeq Version	NM_011948.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus mitogen-activated protein kinase kinase kinase 4 (Map3k4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011948.2

ORF Insert Sequence:

ATGAGAGACG CCATCGCCGA GCCGGTGCCC CCTCCTGCCC TCGCCGACAC CCCTGCAGCC 
GCCATGGAGG AGCTGCGGCC AGCACCGCCG CCACAGCCCG AGCCGGATCC GGAGTGCTGC 
CCAGCGGCGA GGCAGGAGTG CATGTTGGGA GAGTCGGCTC GCAAAAGTAT GGAATCCGAT 
CCAGAGGACT TTTCTGATGA AACAAATACA GAGACTCTCT ACGGCACCTC ACCCCCAAGC 
ACACCTCGAC AGATGAAACG CCTGTCAGCC AAGCACCAGA GGAACAGCGC AGGGAGGCCG 
GCCAGCCGAT CGAACTTGAA AGAAAAAATG AACACACCGA GTCAGTCTCC ACATAAAGAT 
TTGGGGAAGG GAGTGGAGAC CGTGGAAGAA TACAGCTACA AGCAGGAGAA GAAGATTCGA 
GCAACTCTGA GAACAACGGA GCGAGACCAT AAGAAAAATG CACAGTGCTC GTTCATGTTG 
GACTCGGTGG CTGGGTCTTT GCCAAAAAAA TCGATTCCAG ATGTGGATCT CAATAAGCCT 
TACCTCAGTC TCGGCTGTAG CAATGCCAAG CTGCCCGTCT CGATGCCCAT GCCGATAGCC 
AGAACTGCAC GGCAGACTTC CCGGACTGAC TGCCCCGCAG ATCGCTTAAA GTTCTTTGAA 
ACACTGCGCC TTTTGCTAAA GCTTACCTCA GTCTCGAAGA AGAAGGACAG GGAGCAGAGG 
GGACAAGAAA ACACGGCTGC TTTCTGGTTC AACCGATCGA ACGAACTGAT CTGGTTAGAA 
CTGCAGGCCT GGCACGCGGG CCGCACCATC AATGACCAGG ACCTCTTTCT CTACACAGCC 
CGCCAGGCCA TCCCAGACAT CATCAATGAG ATCCTCACCT TCAAAGTTAA CTACGGGAGC 
ATTGCCTTCT CCAGCAATGG AGCCGGTTTC AACGGGCCCT TGGTAGAAGG CCAGTGCAGA 
ACCCCTCAGG AGACAAACCG TGTGGGCTGC TCATCGTACC ACGAGCACCT CCAGCGCCAG 
AGGGTCTCGT TTGAGCAGGT GAAGCGGATA ATGGAGCTGC TGGAGTACAT GGAGGCACTT 
TACCCATCCT TGCAGGCTCT GCAGAAGGAC TATGAACGGT ACGCCGCCAA GGACTTTGAG 
GACAGAGTGC AGGCGCTCTG CCTGTGGCTC AACATCACGA AAGATCTAAA TCAGAAGCTG 
CGGATCATGG GCACCGTGCT GGGCATCAAG AACCTATCAG ACATTGGCTG GCCAGTGTTT 
GAAATCCCCT CCCCTCGGCC GTCCAAGGGC TACGAGCCAG AGGACGAGGT CGAGGACACG 
GAGGTTGAGC TGAGGGAGCT GGAGAGCGGG ACGGAGGAGA GTGACGAGGA GCCAACCCCC 
AGTCCGAGGG TGCCAGAGCT CAGGCTGTCC ACAGACGCCA TCTTGGACAG TCGCTCCCAG 
GGCTGCGTCT CCAGGAAGCT GGAGAGGCTC GAGTCAGAGG AAGATTCCAT AGGCTGGGGG 
ACAGCGGACT GTGGCCCTGA AGCCAGCAGG CATTGTTTGA CTTCTATCTA TAGACCATTC 
GTGGACAAAG CACTGAAGCA AATGGGGCTA AGAAAGTTAA TTTTACGACT TCATAAGCTT 
ATGAATGGGT CCTTGCAAAG AGCTCGTGTA GCTCTGGTGA AGGACGACCG TCCAGTGGAG 
TTCTCTGACT TTCCAGGTCC CATGTGGGGC TCGGATTATG TGCAGTTGTC GGGAACACCT 
CCTTCCTCAG AGCAGAAGTG TAGCGCTGTG TCCTGGGAAG AACTGAGAGC CATGGACCTG 
CCTTCCTTTG AGCCCGCCTT CCTGGTGCTC TGTCGGGTCC TGCTGAACGT GATCCACGAG 
TGCCTGAAGC TGCGGCTGGA ACAGAGGCCT GCCGGGGAGC CTTCCCTCTT GAGTATCAAA 
CAGCTAGTGC GAGAGTGTAA AGAGGTCCTA AAGGGCGGGC TCCTGATGAA GCAGTATTAC 
CAGTTCATGC TGCAGGAGGT CCTGGGCGGA CTGGAGAAGA CCGACTGCAA CATGGATGCC 
TTTGAGGAGG ACCTGCAGAA GATGCTGATG GTGTATTTTG ATTACATGAG AAGCTGGATC 
CAAATGCTAC AGCAGTTACC TCAGGCTTCC CATAGCTTAA AAAACCTGCT AGAAGAGGAA 
TGGAATTTCA CCAAAGAAAT AACCCATTAT ATCCGTGGCG GAGAAGCGCA GGCTGGAAAG 
CTTTTCTGTG ACATCGCAGG GATGCTGCTG AAATCCACAG GGAGCTTTCT GGAATCCGGC 
CTGCAGGAGA GCTGTGCTGA GCTGTGGACC AGCGCCGACG ACAACGGTGC TGCCGACGAG 
CTAAGGAGAT CTGTCATCGA GATCAGCCGA GCACTCAAGG AGCTCTTCCA CGAAGCCAGG 
GAAAGAGCCT CCAAGGCCCT GGGCTTTGCT AAAATGCTGA GGAAGGACCT AGAAATAGCA 
GCAGAGTTCG TGCTATCTGC ATCAGCCCGA GAGCTCCTGG ACGCTCTGAA AGCAAAGCAG 
TATGTTAAGG TACAGATTCC CGGGTTAGAG AATTTGCACG TGTTTGTCCC CGACAGCCTC 
GCTGAGGAGA AGAAAATTAT TTTGCAGCTA CTCAATGCTG CCACAGGAAA GGACTGCTCA 
AAGGATCCAG ACGACGTCTT CATGGATGCC TTCCTGCTCC TGACCAAGCA TGGGGACCGA 
GCCCGTGACT CAGAAGATGG CTGGGGCACA TGGGAAGCTC GGGCTGTCAA AATTGTGCCT 
CAGGTGGAGA CTGTGGACAC CCTGAGAAGC ATGCAGGTGG ACAACCTTCT GCTGGTTGTC 
ATGGAGTCTG CTCACCTCGT ACTTCAGAGA AAAGCCTTCC AGCAGTCCAT TGAGGGGCTG 
ATGACTGTAC GCCATGAGCA GACATCTAGC CAGCCCATCA TCGCCAAAGG TTTGCAGCAG 
CTCAAGAACG ATGCACTTGA GCTATGCAAC AGAATCAGCG ATGCCATCGA CCGTGTGGAC 
CACATGTTCA CCCTGGAGTT CGATGCTGAG GTCGAGGAGT CTGAGTCGGC CACGCTGCAG 
CAGTACTACC GAGAAGCCAT GATTCAGGGC TACAACTTTG GGTTTGAGTA TCATAAAGAA 
GTTGTTCGTT TGATGTCTGG GGAATTCAGG CAGAAGATAG GAGACAAATA TATAAGCTTC 
GCCCAGAAGT GGATGAATTA CGTGCTGACC AAATGCGAGA GCGGCAGAGG CACAAGACCC 
AGATGGGCCA CCCAAGGATT TGATTTCCTA CAAGCCATTG AACCTGCCTT TATTTCAGCT 
TTACCAGAAG ATGACTTCTT GAGTTTGCAA GCCCTGATGA ATGAGTGCAT CGGGCACGTC 
ATAGGAAAGC CACACAGCCC TGTCACAGCT ATCCATCGGA ACAGCCCCCG CCCTGTGAAG 
GTGCCCCGAT GCCACAGTGA CCCTCCTAAC CCTCACCTCA TCATCCCGAC TCCAGAGGGA 
TTCAGCACCC GGAGCGTGCC TTCCGACGCT CGGACCCATG GCAACTCTGT TGCTGCTGCT 
GCTGCTGTTG CTGCCGCCGC CACCACTGCT GCTGGCCGCC CTGGCCCAGG TGGTGGTGAC 
TCTGTGCCAG CCAAACCTGT CAACACTGCC CCTGATACCA GGGGTTCCAG TGTCCCTGAA 
AACGACCGCT TGGCCTCCAT AGCTGCAGAA CTGCAGTTCA GGTCTCTGAG TCGGCACTCA 
AGCCCCACGG AAGAGCGAGA CGAGCCAGCG TATCCTCGGA GTGACTCAAG TGGATCAACT 
CGGAGAAGCT GGGAACTTCG AACACTCATC AGCCAGACCA AAGACTCGGC CTCTAAGCAG 
GGGCCCATAG AAGCTATCCA GAAGTCAGTC CGACTGTTTG AAGAGAGGAG GTATCGAGAG 
ATGAGGAGAA AGAATATCAT CGGCCAAGTG TGCGATACCC CTAAGTCCTA TGATAACGTC 
ATGCATGTTG GACTGAGGAA GGTGACATTT AAGTGGCAAA GAGGAAACAA AATTGGAGAA 
GGACAGTATG GAAAAGTATA CACCTGCATC AGTGTTGACA CAGGGGAGCT GATGGCCATG 
AAGGAGATTC GATTTCAGCC TAACGACCAC AAGACTATCA AGGAGACTGC AGACGAGTTG 
AAAATATTTG AAGGCATCAA GCACCCCAAC CTGGTCCGGT ATTTTGGCGT GGAGCTTCAC 
AGGGAAGAGA TGTACATCTT CATGGAGTAC TGTGATGAGG GTACACTAGA GGAGGTGTCA 
CGACTGGGCC TGCAGGAGCA CGTCATCAGG TTATATACCA AGCAGATCAC TGTCGCCATC 
AACGTCCTCC ATGAGCACGG CATCGTTCAC CGAGACATCA AAGGTGCCAA TATCTTCCTT 
ACGTCATCTG GACTAATCAA GCTGGGAGAT TTTGGATGCT CTGTAAAACT TAAAAACAAC 
GCCCAGACCA TGCCCGGAGA GGTGAACAGC ACCCTAGGGA CAGCAGCTTA CATGGCCCCT 
GAAGTTATTA CCCGAGCCAA AGGAGAAGGC CACGGACGTG CGGCAGATAT CTGGAGTCTG 
GGGTGCGTCG TCATAGAGAT GGTGACTGGC AAGCGGCCTT GGCATGAGTA TGAACACAAC 
TTTCAGATTA TGTACAAGGT GGGGATGGGA CACAAGCCAC CAATCCCGGA AAGGCTAAGC 
CCTGAAGGAA AGGCCTTTCT CTCGCACTGC CTGGAAAGTG ACCCGAAGAT ACGGTGGACA 
GCCAGCCAGC TCCTCGACCA CGCTTTTGTC AAGGTTTGCA CAGATGAAGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K4 ( XM_030249819.1 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_030105679.1 Mus musculus mitogen-activated protein kinase kinase kinase 4 (Map3k4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu87205
Clone ID Related Accession (Same CDS sequence)	XM_030249819.1
Accession Version	XM_030249819.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4653bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	mitogen-activated protein kinase kinase kinase 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGGAG AGTCGGCTCG CAAAAGTATG GAATCCGATC CAGAGGACTT TTCTGATGAA 
ACAAATACAG AGACTCTCTA CGGCACCTCA CCCCCAAGCA CACCTCGACA GATGAAACGC 
CTGTCAGCCA AGCACCAGAG GAACAGCGCA GGGAGGCCGG CCAGCCGATC GAACTTGAAA 
GAAAAAATGA ACACACCGAG TCAGTCTCCA CATAAAGATT TGGGGAAGGG AGTGGAGACC 
GTGGAAGAAT ACAGCTACAA GCAGGAGAAG AAGATTCGAG CAACTCTGAG AACAACGGAG 
CGAGACCATA AGAAAAATGC ACAGTGCTCG TTCATGTTGG ACTCGGTGGC TGGGTCTTTG 
CCAAAAAAAT CGATTCCAGA TGTGGATCTC AATAAGCCTT ACCTCAGTCT CGGCTGTAGC 
AATGCCAAGC TGCCCGTCTC GATGCCCATG CCGATAGCCA GAACTGCACG GCAGACTTCC 
CGGACTGACT GCCCCGCAGA TCGCTTAAAG TTCTTTGAAA CACTGCGCCT TTTGCTAAAG 
CTTACCTCAG TCTCGAAGAA GAAGGACAGG GAGCAGAGGG GACAAGAAAA CACGGCTGCT 
TTCTGGTTCA ACCGATCGAA CGAACTGATC TGGTTAGAAC TGCAGGCCTG GCACGCGGGC 
CGCACCATCA ATGACCAGGA CCTCTTTCTC TACACAGCCC GCCAGGCCAT CCCAGACATC 
ATCAATGAGA TCCTCACCTT CAAAGTTAAC TACGGGAGCA TTGCCTTCTC CAGCAATGGA 
GCCGGTTTCA ACGGGCCCTT GGTAGAAGGC CAGTGCAGAA CCCCTCAGGA GACAAACCGT 
GTGGGCTGCT CATCGTACCA CGAGCACCTC CAGCGCCAGA GGGTCTCGTT TGAGCAGGTG 
AAGCGGATAA TGGAGCTGCT GGAGTACATG GAGGCACTTT ACCCATCCTT GCAGGCTCTG 
CAGAAGGACT ATGAACGGTA CGCCGCCAAG GACTTTGAGG ACAGAGTGCA GGCGCTCTGC 
CTGTGGCTCA ACATCACGAA AGATCTAAAT CAGAAGCTGC GGATCATGGG CACCGTGCTG 
GGCATCAAGA ACCTATCAGA CATTGGCTGG CCAGTGTTTG AAATCCCCTC CCCTCGGCCG 
TCCAAGGGCT ACGAGCCAGA GGACGAGGTC GAGGACACGG AGGTTGAGCT GAGGGAGCTG 
GAGAGCGGGA CGGAGGAGAG TGACGAGGAG CCAACCCCCA GTCCGAGGGT GCCAGAGCTC 
AGGCTGTCCA CAGACGCCAT CTTGGACAGT CGCTCCCAGG GCTGCGTCTC CAGGAAGCTG 
GAGAGGCTCG AGTCAGAGGA AGATTCCATA GGCTGGGGGA CAGCGGACTG TGGCCCTGAA 
GCCAGCAGGC ATTGTTTGAC TTCTATCTAT AGACCATTCG TGGACAAAGC ACTGAAGCAA 
ATGGGGCTAA GAAAGTTAAT TTTACGACTT CATAAGCTTA TGAATGGGTC CTTGCAAAGA 
GCTCGTGTAG CTCTGGTGAA GGACGACCGT CCAGTGGAGT TCTCTGACTT TCCAGGTCCC 
ATGTGGGGCT CGGATTATGT GCAGTTGTCG GGAACACCTC CTTCCTCAGA GCAGAAGTGT 
AGCGCTGTGT CCTGGGAAGA ACTGAGAGCC ATGGACCTGC CTTCCTTTGA GCCCGCCTTC 
CTGGTGCTCT GTCGGGTCCT GCTGAACGTG ATCCACGAGT GCCTGAAGCT GCGGCTGGAA 
CAGAGGCCTG CCGGGGAGCC TTCCCTCTTG AGTATCAAAC AGCTAGTGCG AGAGTGTAAA 
GAGGTCCTAA AGGGCGGGCT CCTGATGAAG CAGTATTACC AGTTCATGCT GCAGGAGGTC 
CTGGGCGGAC TGGAGAAGAC CGACTGCAAC ATGGATGCCT TTGAGGAGGA CCTGCAGAAG 
ATGCTGATGG TGTATTTTGA TTACATGAGA AGCTGGATCC AAATGCTACA GCAGTTACCT 
CAGGCTTCCC ATAGCTTAAA AAACCTGCTA GAAGAGGAAT GGAATTTCAC CAAAGAAATA 
ACCCATTATA TCCGTGGCGG AGAAGCGCAG GCTGGAAAGC TTTTCTGTGA CATCGCAGGG 
ATGCTGCTGA AATCCACAGG GAGCTTTCTG GAATCCGGCC TGCAGGAGAG CTGTGCTGAG 
CTGTGGACCA GCGCCGACGA CAACGGTGCT GCCGACGAGC TAAGGAGATC TGTCATCGAG 
ATCAGCCGAG CACTCAAGGA GCTCTTCCAC GAAGCCAGGG AAAGAGCCTC CAAGGCCCTG 
GGCTTTGCTA AAATGCTGAG GAAGGACCTA GAAATAGCAG CAGAGTTCGT GCTATCTGCA 
TCAGCCCGAG AGCTCCTGGA CGCTCTGAAA GCAAAGCAGT ATGTTAAGGT ACAGATTCCC 
GGGTTAGAGA ATTTGCACGT GTTTGTCCCC GACAGCCTCG CTGAGGAGAA GAAAATTATT 
TTGCAGCTAC TCAATGCTGC CACAGGAAAG GACTGCTCAA AGGATCCAGA CGACGTCTTC 
ATGGATGCCT TCCTGCTCCT GACCAAGCAT GGGGACCGAG CCCGTGACTC AGAAGATGGC 
TGGGGCACAT GGGAAGCTCG GGCTGTCAAA ATTGTGCCTC AGGTGGAGAC TGTGGACACC 
CTGAGAAGCA TGCAGGTGGA CAACCTTCTG CTGGTTGTCA TGGAGTCTGC TCACCTCGTA 
CTTCAGAGAA AAGCCTTCCA GCAGTCCATT GAGGGGCTGA TGACTGTACG CCATGAGCAG 
ACATCTAGCC AGCCCATCAT CGCCAAAGGT TTGCAGCAGC TCAAGAACGA TGCACTTGAG 
CTATGCAACA GAATCAGCGA TGCCATCGAC CGTGTGGACC ACATGTTCAC CCTGGAGTTC 
GATGCTGAGG TCGAGGAGTC TGAGTCGGCC ACGCTGCAGC AGTACTACCG AGAAGCCATG 
ATTCAGGGCT ACAACTTTGG GTTTGAGTAT CATAAAGAAG TTGTTCGTTT GATGTCTGGG 
GAATTCAGGC AGAAGATAGG AGACAAATAT ATAAGCTTCG CCCAGAAGTG GATGAATTAC 
GTGCTGACCA AATGCGAGAG CGGCAGAGGC ACAAGACCCA GATGGGCCAC CCAAGGATTT 
GATTTCCTAC AAGCCATTGA ACCTGCCTTT ATTTCAGCTT TACCAGAAGA TGACTTCTTG 
AGTTTGCAAG CCCTGATGAA TGAGTGCATC GGGCACGTCA TAGGAAAGCC ACACAGCCCT 
GTCACAGCTA TCCATCGGAA CAGCCCCCGC CCTGTGAAGG TGCCCCGATG CCACAGTGAC 
CCTCCTAACC CTCACCTCAT CATCCCGACT CCAGAGGGAT TCAGCACCCG GAGCGTGCCT 
TCCGACGCTC GGACCCATGG CAACTCTGTT GCTGCTGCTG CTGCTGTTGC TGCCGCCGCC 
ACCACTGCTG CTGGCCGCCC TGGCCCAGGT GGTGGTGACT CTGTGCCAGC CAAACCTGTC 
AACACTGCCC CTGATACCAG GGGTTCCAGT GTCCCTGAAA ACGACCGCTT GGCCTCCATA 
GCTGCAGAAC TGCAGTTCAG GTCTCTGAGT CGGCACTCAA GCCCCACGGA AGAGCGAGAC 
GAGCCAGCGT ATCCTCGGAG TGACTCAAGT GGATCAACTC GGAGAAGCTG GGAACTTCGA 
ACACTCATCA GCCAGACCAA AGACTCGGCC TCTAAGCAGG GGCCCATAGA AGCTATCCAG 
AAGTCAGTCC GACTGTTTGA AGAGAGGAGG TATCGAGAGA TGAGGAGAAA GAATATCATC 
GGCCAAGTGT GCGATACCCC TAAGTCCTAT GATAACGTCA TGCATGTTGG ACTGAGGAAG 
GTGACATTTA AGTGGCAAAG AGGAAACAAA ATTGGAGAAG GACAGTATGG AAAAGTATAC 
ACCTGCATCA GTGTTGACAC AGGGGAGCTG ATGGCCATGA AGGAGATTCG ATTTCAGCCT 
AACGACCACA AGACTATCAA GGAGACTGCA GACGAGTTGA AAATATTTGA AGGCATCAAG 
CACCCCAACC TGGTCCGGTA TTTTGGCGTG GAGCTTCACA GGGAAGAGAT GTACATCTTC 
ATGGAGTACT GTGATGAGGG TACACTAGAG GAGGTGTCAC GACTGGGCCT GCAGGAGCAC 
GTCATCAGGT TATATACCAA GCAGATCACT GTCGCCATCA ACGTCCTCCA TGAGCACGGC 
ATCGTTCACC GAGACATCAA AGGTGCCAAT ATCTTCCTTA CGTCATCTGG ACTAATCAAG 
CTGGGAGATT TTGGATGCTC TGTAAAACTT AAAAACAACG CCCAGACCAT GCCCGGAGAG 
GTGAACAGCA CCCTAGGGAC AGCAGCTTAC ATGGCCCCTG AAGTTATTAC CCGAGCCAAA 
GGAGAAGGCC ACGGACGTGC GGCAGATATC TGGAGTCTGG GGTGCGTCGT CATAGAGATG 
GTGACTGGCA AGCGGCCTTG GCATGAGTAT GAACACAACT TTCAGATTAT GTACAAGGTG 
GGGATGGGAC ACAAGCCACC AATCCCGGAA AGGCTAAGCC CTGAAGGAAA GGCCTTTCTC 
TCGCACTGCC TGGAAAGTGA CCCGAAGATA CGGTGGACAG CCAGCCAGCT CCTCGACCAC 
GCTTTTGTCA AGGTTTGCAC AGATGAAGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105679.1
CDS	2595..7247
Translation	MLGESARKSMESDPEDFSDETNTETLYGTSPPSTPRQMKRLSAKHQRNSAGRPASRSNLKEKMNTPSQSPHKDLGKGVETVEEYSYKQEKKIRATLRTTERDHKKNAQCSFMLDSVAGSLPKKSIPDVDLNKPYLSLGCSNAKLPVSMPMPIARTARQTSRTDCPADRLKFFETLRLLLKLTSVSKKKDREQRGQENTAAFWFNRSNELIWLELQAWHAGRTINDQDLFLYTARQAIPDIINEILTFKVNYGSIAFSSNGAGFNGPLVEGQCRTPQETNRVGCSSYHEHLQRQRVSFEQVKRIMELLEYMEALYPSLQALQKDYERYAAKDFEDRVQALCLWLNITKDLNQKLRIMGTVLGIKNLSDIGWPVFEIPSPRPSKGYEPEDEVEDTEVELRELESGTEESDEEPTPSPRVPELRLSTDAILDSRSQGCVSRKLERLESEEDSIGWGTADCGPEASRHCLTSIYRPFVDKALKQMGLRKLILRLHKLMNGSLQRARVALVKDDRPVEFSDFPGPMWGSDYVQLSGTPPSSEQKCSAVSWEELRAMDLPSFEPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLQEVLGGLEKTDCNMDAFEEDLQKMLMVYFDYMRSWIQMLQQLPQASHSLKNLLEEEWNFTKEITHYIRGGEAQAGKLFCDIAGMLLKSTGSFLESGLQESCAELWTSADDNGAADELRRSVIEISRALKELFHEARERASKALGFAKMLRKDLEIAAEFVLSASARELLDALKAKQYVKVQIPGLENLHVFVPDSLAEEKKIILQLLNAATGKDCSKDPDDVFMDAFLLLTKHGDRARDSEDGWGTWEARAVKIVPQVETVDTLRSMQVDNLLLVVMESAHLVLQRKAFQQSIEGLMTVRHEQTSSQPIIAKGLQQLKNDALELCNRISDAIDRVDHMFTLEFDAEVEESESATLQQYYREAMIQGYNFGFEYHKEVVRLMSGEFRQKIGDKYISFAQKWMNYVLTKCESGRGTRPRWATQGFDFLQAIEPAFISALPEDDFLSLQALMNECIGHVIGKPHSPVTAIHRNSPRPVKVPRCHSDPPNPHLIIPTPEGFSTRSVPSDARTHGNSVAAAAAVAAAATTAAGRPGPGGGDSVPAKPVNTAPDTRGSSVPENDRLASIAAELQFRSLSRHSSPTEERDEPAYPRSDSSGSTRRSWELRTLISQTKDSASKQGPIEAIQKSVRLFEERRYREMRRKNIIGQVCDTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCISVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSRLGLQEHVIRLYTKQITVAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLKNNAQTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVVIEMVTGKRPWHEYEHNFQIMYKVGMGHKPPIPERLSPEGKAFLSHCLESDPKIRWTASQLLDHAFVKVCTDEE

Target ORF information:

RefSeq Version	XM_030249819.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mitogen-activated protein kinase kinase kinase 4 (Map3k4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249819.1

ORF Insert Sequence:

ATGTTGGGAG AGTCGGCTCG CAAAAGTATG GAATCCGATC CAGAGGACTT TTCTGATGAA 
ACAAATACAG AGACTCTCTA CGGCACCTCA CCCCCAAGCA CACCTCGACA GATGAAACGC 
CTGTCAGCCA AGCACCAGAG GAACAGCGCA GGGAGGCCGG CCAGCCGATC GAACTTGAAA 
GAAAAAATGA ACACACCGAG TCAGTCTCCA CATAAAGATT TGGGGAAGGG AGTGGAGACC 
GTGGAAGAAT ACAGCTACAA GCAGGAGAAG AAGATTCGAG CAACTCTGAG AACAACGGAG 
CGAGACCATA AGAAAAATGC ACAGTGCTCG TTCATGTTGG ACTCGGTGGC TGGGTCTTTG 
CCAAAAAAAT CGATTCCAGA TGTGGATCTC AATAAGCCTT ACCTCAGTCT CGGCTGTAGC 
AATGCCAAGC TGCCCGTCTC GATGCCCATG CCGATAGCCA GAACTGCACG GCAGACTTCC 
CGGACTGACT GCCCCGCAGA TCGCTTAAAG TTCTTTGAAA CACTGCGCCT TTTGCTAAAG 
CTTACCTCAG TCTCGAAGAA GAAGGACAGG GAGCAGAGGG GACAAGAAAA CACGGCTGCT 
TTCTGGTTCA ACCGATCGAA CGAACTGATC TGGTTAGAAC TGCAGGCCTG GCACGCGGGC 
CGCACCATCA ATGACCAGGA CCTCTTTCTC TACACAGCCC GCCAGGCCAT CCCAGACATC 
ATCAATGAGA TCCTCACCTT CAAAGTTAAC TACGGGAGCA TTGCCTTCTC CAGCAATGGA 
GCCGGTTTCA ACGGGCCCTT GGTAGAAGGC CAGTGCAGAA CCCCTCAGGA GACAAACCGT 
GTGGGCTGCT CATCGTACCA CGAGCACCTC CAGCGCCAGA GGGTCTCGTT TGAGCAGGTG 
AAGCGGATAA TGGAGCTGCT GGAGTACATG GAGGCACTTT ACCCATCCTT GCAGGCTCTG 
CAGAAGGACT ATGAACGGTA CGCCGCCAAG GACTTTGAGG ACAGAGTGCA GGCGCTCTGC 
CTGTGGCTCA ACATCACGAA AGATCTAAAT CAGAAGCTGC GGATCATGGG CACCGTGCTG 
GGCATCAAGA ACCTATCAGA CATTGGCTGG CCAGTGTTTG AAATCCCCTC CCCTCGGCCG 
TCCAAGGGCT ACGAGCCAGA GGACGAGGTC GAGGACACGG AGGTTGAGCT GAGGGAGCTG 
GAGAGCGGGA CGGAGGAGAG TGACGAGGAG CCAACCCCCA GTCCGAGGGT GCCAGAGCTC 
AGGCTGTCCA CAGACGCCAT CTTGGACAGT CGCTCCCAGG GCTGCGTCTC CAGGAAGCTG 
GAGAGGCTCG AGTCAGAGGA AGATTCCATA GGCTGGGGGA CAGCGGACTG TGGCCCTGAA 
GCCAGCAGGC ATTGTTTGAC TTCTATCTAT AGACCATTCG TGGACAAAGC ACTGAAGCAA 
ATGGGGCTAA GAAAGTTAAT TTTACGACTT CATAAGCTTA TGAATGGGTC CTTGCAAAGA 
GCTCGTGTAG CTCTGGTGAA GGACGACCGT CCAGTGGAGT TCTCTGACTT TCCAGGTCCC 
ATGTGGGGCT CGGATTATGT GCAGTTGTCG GGAACACCTC CTTCCTCAGA GCAGAAGTGT 
AGCGCTGTGT CCTGGGAAGA ACTGAGAGCC ATGGACCTGC CTTCCTTTGA GCCCGCCTTC 
CTGGTGCTCT GTCGGGTCCT GCTGAACGTG ATCCACGAGT GCCTGAAGCT GCGGCTGGAA 
CAGAGGCCTG CCGGGGAGCC TTCCCTCTTG AGTATCAAAC AGCTAGTGCG AGAGTGTAAA 
GAGGTCCTAA AGGGCGGGCT CCTGATGAAG CAGTATTACC AGTTCATGCT GCAGGAGGTC 
CTGGGCGGAC TGGAGAAGAC CGACTGCAAC ATGGATGCCT TTGAGGAGGA CCTGCAGAAG 
ATGCTGATGG TGTATTTTGA TTACATGAGA AGCTGGATCC AAATGCTACA GCAGTTACCT 
CAGGCTTCCC ATAGCTTAAA AAACCTGCTA GAAGAGGAAT GGAATTTCAC CAAAGAAATA 
ACCCATTATA TCCGTGGCGG AGAAGCGCAG GCTGGAAAGC TTTTCTGTGA CATCGCAGGG 
ATGCTGCTGA AATCCACAGG GAGCTTTCTG GAATCCGGCC TGCAGGAGAG CTGTGCTGAG 
CTGTGGACCA GCGCCGACGA CAACGGTGCT GCCGACGAGC TAAGGAGATC TGTCATCGAG 
ATCAGCCGAG CACTCAAGGA GCTCTTCCAC GAAGCCAGGG AAAGAGCCTC CAAGGCCCTG 
GGCTTTGCTA AAATGCTGAG GAAGGACCTA GAAATAGCAG CAGAGTTCGT GCTATCTGCA 
TCAGCCCGAG AGCTCCTGGA CGCTCTGAAA GCAAAGCAGT ATGTTAAGGT ACAGATTCCC 
GGGTTAGAGA ATTTGCACGT GTTTGTCCCC GACAGCCTCG CTGAGGAGAA GAAAATTATT 
TTGCAGCTAC TCAATGCTGC CACAGGAAAG GACTGCTCAA AGGATCCAGA CGACGTCTTC 
ATGGATGCCT TCCTGCTCCT GACCAAGCAT GGGGACCGAG CCCGTGACTC AGAAGATGGC 
TGGGGCACAT GGGAAGCTCG GGCTGTCAAA ATTGTGCCTC AGGTGGAGAC TGTGGACACC 
CTGAGAAGCA TGCAGGTGGA CAACCTTCTG CTGGTTGTCA TGGAGTCTGC TCACCTCGTA 
CTTCAGAGAA AAGCCTTCCA GCAGTCCATT GAGGGGCTGA TGACTGTACG CCATGAGCAG 
ACATCTAGCC AGCCCATCAT CGCCAAAGGT TTGCAGCAGC TCAAGAACGA TGCACTTGAG 
CTATGCAACA GAATCAGCGA TGCCATCGAC CGTGTGGACC ACATGTTCAC CCTGGAGTTC 
GATGCTGAGG TCGAGGAGTC TGAGTCGGCC ACGCTGCAGC AGTACTACCG AGAAGCCATG 
ATTCAGGGCT ACAACTTTGG GTTTGAGTAT CATAAAGAAG TTGTTCGTTT GATGTCTGGG 
GAATTCAGGC AGAAGATAGG AGACAAATAT ATAAGCTTCG CCCAGAAGTG GATGAATTAC 
GTGCTGACCA AATGCGAGAG CGGCAGAGGC ACAAGACCCA GATGGGCCAC CCAAGGATTT 
GATTTCCTAC AAGCCATTGA ACCTGCCTTT ATTTCAGCTT TACCAGAAGA TGACTTCTTG 
AGTTTGCAAG CCCTGATGAA TGAGTGCATC GGGCACGTCA TAGGAAAGCC ACACAGCCCT 
GTCACAGCTA TCCATCGGAA CAGCCCCCGC CCTGTGAAGG TGCCCCGATG CCACAGTGAC 
CCTCCTAACC CTCACCTCAT CATCCCGACT CCAGAGGGAT TCAGCACCCG GAGCGTGCCT 
TCCGACGCTC GGACCCATGG CAACTCTGTT GCTGCTGCTG CTGCTGTTGC TGCCGCCGCC 
ACCACTGCTG CTGGCCGCCC TGGCCCAGGT GGTGGTGACT CTGTGCCAGC CAAACCTGTC 
AACACTGCCC CTGATACCAG GGGTTCCAGT GTCCCTGAAA ACGACCGCTT GGCCTCCATA 
GCTGCAGAAC TGCAGTTCAG GTCTCTGAGT CGGCACTCAA GCCCCACGGA AGAGCGAGAC 
GAGCCAGCGT ATCCTCGGAG TGACTCAAGT GGATCAACTC GGAGAAGCTG GGAACTTCGA 
ACACTCATCA GCCAGACCAA AGACTCGGCC TCTAAGCAGG GGCCCATAGA AGCTATCCAG 
AAGTCAGTCC GACTGTTTGA AGAGAGGAGG TATCGAGAGA TGAGGAGAAA GAATATCATC 
GGCCAAGTGT GCGATACCCC TAAGTCCTAT GATAACGTCA TGCATGTTGG ACTGAGGAAG 
GTGACATTTA AGTGGCAAAG AGGAAACAAA ATTGGAGAAG GACAGTATGG AAAAGTATAC 
ACCTGCATCA GTGTTGACAC AGGGGAGCTG ATGGCCATGA AGGAGATTCG ATTTCAGCCT 
AACGACCACA AGACTATCAA GGAGACTGCA GACGAGTTGA AAATATTTGA AGGCATCAAG 
CACCCCAACC TGGTCCGGTA TTTTGGCGTG GAGCTTCACA GGGAAGAGAT GTACATCTTC 
ATGGAGTACT GTGATGAGGG TACACTAGAG GAGGTGTCAC GACTGGGCCT GCAGGAGCAC 
GTCATCAGGT TATATACCAA GCAGATCACT GTCGCCATCA ACGTCCTCCA TGAGCACGGC 
ATCGTTCACC GAGACATCAA AGGTGCCAAT ATCTTCCTTA CGTCATCTGG ACTAATCAAG 
CTGGGAGATT TTGGATGCTC TGTAAAACTT AAAAACAACG CCCAGACCAT GCCCGGAGAG 
GTGAACAGCA CCCTAGGGAC AGCAGCTTAC ATGGCCCCTG AAGTTATTAC CCGAGCCAAA 
GGAGAAGGCC ACGGACGTGC GGCAGATATC TGGAGTCTGG GGTGCGTCGT CATAGAGATG 
GTGACTGGCA AGCGGCCTTG GCATGAGTAT GAACACAACT TTCAGATTAT GTACAAGGTG 
GGGATGGGAC ACAAGCCACC AATCCCGGAA AGGCTAAGCC CTGAAGGAAA GGCCTTTCTC 
TCGCACTGCC TGGAAAGTGA CCCGAAGATA CGGTGGACAG CCAGCCAGCT CCTCGACCAC 
GCTTTTGTCA AGGTTTGCAC AGATGAAGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K4 ( NM_001357722.1 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_001344651.1 Mus musculus mitogen-activated protein kinase kinase kinase 4 (Map3k4), transcript variant 2, mRNA.

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CloneID	OMu52902
Clone ID Related Accession (Same CDS sequence)	NM_001357722.1 , XM_006523329.3
Accession Version	NM_001357722.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4638bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Mus musculus(house mouse)
Product	mitogen-activated protein kinase kinase kinase 4 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC136921.8 and CO040910.1. On Nov 4, 2017 this sequence version replaced XM_006523329.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U85608.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGAGACG CCATCGCCGA GCCGGTGCCC CCTCCTGCCC TCGCCGACAC CCCTGCAGCC 
GCCATGGAGG AGCTGCGGCC AGCACCGCCG CCACAGCCCG AGCCGGATCC GGAGTGCTGC 
CCAGCGGCGA GGCAGGAGTG CATGTTGGGA GAGTCGGCTC GCAAAAGTAT GGAATCCGAT 
CCAGAGGACT TTTCTGATGA AACAAATACA GAGACTCTCT ACGGCACCTC ACCCCCAAGC 
ACACCTCGAC AGATGAAACG CCTGTCAGCC AAGCACCAGA GGAACAGCGC AGGGAGGCCG 
GCCAGCCGAT CGAACTTGAA AGAAAAAATG AACACACCGA GTCAGTCTCC ACATAAAGAT 
TTGGGGAAGG GAGTGGAGAC CGTGGAAGAA TACAGCTACA AGCAGGAGAA GAAGATTCGA 
GCAACTCTGA GAACAACGGA GCGAGACCAT AAGAAAAATG CACAGTGCTC GTTCATGTTG 
GACTCGGTGG CTGGGTCTTT GCCAAAAAAA TCGATTCCAG ATGTGGATCT CAATAAGCCT 
TACCTCAGTC TCGGCTGTAG CAATGCCAAG CTGCCCGTCT CGATGCCCAT GCCGATAGCC 
AGAACTGCAC GGCAGACTTC CCGGACTGAC TGCCCCGCAG ATCGCTTAAA GTTCTTTGAA 
ACACTGCGCC TTTTGCTAAA GCTTACCTCA GTCTCGAAGA AGAAGGACAG GGAGCAGAGG 
GGACAAGAAA ACACGGCTGC TTTCTGGTTC AACCGATCGA ACGAACTGAT CTGGTTAGAA 
CTGCAGGCCT GGCACGCGGG CCGCACCATC AATGACCAGG ACCTCTTTCT CTACACAGCC 
CGCCAGGCCA TCCCAGACAT CATCAATGAG ATCCTCACCT TCAAAGTTAA CTACGGGAGC 
ATTGCCTTCT CCAGCAATGG AGCCGGTTTC AACGGGCCCT TGGTAGAAGG CCAGTGCAGA 
ACCCCTCAGG AGACAAACCG TGTGGGCTGC TCATCGTACC ACGAGCACCT CCAGCGCCAG 
AGGGTCTCGT TTGAGCAGGT GAAGCGGATA ATGGAGCTGC TGGAGTACAT GGAGGCACTT 
TACCCATCCT TGCAGGCTCT GCAGAAGGAC TATGAACGGT ACGCCGCCAA GGACTTTGAG 
GACAGAGTGC AGGCGCTCTG CCTGTGGCTC AACATCACGA AAGATCTAAA TCAGAAGCTG 
CGGATCATGG GCACCGTGCT GGGCATCAAG AACCTATCAG ACATTGGCTG GCCAGTGTTT 
GAAATCCCCT CCCCTCGGCC GTCCAAGGGC TACGAGCCAG AGGACGAGGT CGAGGACACG 
GAGGTTGAGC TGAGGGAGCT GGAGAGCGGG ACGGAGGAGA GTGACGAGGA GCCAACCCCC 
AGTCCGAGGG TGCCAGAGCT CAGGCTGTCC ACAGACGCCA TCTTGGACAG TCGCTCCCAG 
GGCTGCGTCT CCAGGAAGCT GGAGAGGCTC GAGTCAGAGG AAGATTCCAT AGGCTGGGGG 
ACAGCGGACT GTGGCCCTGA AGCCAGCAGG CATTGTTTGA CTTCTATCTA TAGACCATTC 
GTGGACAAAG CACTGAAGCA AATGGGGCTA AGAAAGTTAA TTTTACGACT TCATAAGCTT 
ATGAATGGGT CCTTGCAAAG AGCTCGTGTA GCTCTGGTGA AGGACGACCG TCCAGTGGAG 
TTCTCTGACT TTCCAGGTCC CATGTGGGGC TCGGATTATG TGCAGTTGTC GGGAACACCT 
CCTTCCTCAG AGCAGAAGTG TAGCGCTGTG TCCTGGGAAG AACTGAGAGC CATGGACCTG 
CCTTCCTTTG AGCCCGCCTT CCTGGTGCTC TGTCGGGTCC TGCTGAACGT GATCCACGAG 
TGCCTGAAGC TGCGGCTGGA ACAGAGGCCT GCCGGGGAGC CTTCCCTCTT GAGTATCAAA 
CAGCTAGTGC GAGAGTGTAA AGAGGTCCTA AAGGGCGGGC TCCTGATGAA GCAGTATTAC 
CAGTTCATGC TGCAGGAGGT CCTGGGCGGA CTGGAGAAGA CCGACTGCAA CATGGATGCC 
TTTGAGGAGG ACCTGCAGAA GATGCTGATG GTGTATTTTG ATTACATGAG AAGCTGGATC 
CAAATGCTAC AGCAGTTACC TCAGGCTTCC CATAGCTTAA AAAACCTGCT AGAAGAGGAA 
TGGAATTTCA CCAAAGAAAT AACCCATTAT ATCCGTGGCG GAGAAGCGCA GGCTGGAAAG 
CTTTTCTGTG ACATCGCAGG GATGCTGCTG AAATCCACAG GGAGCTTTCT GGAATCCGGC 
CTGCAGGAGA GCTGTGCTGA GCTGTGGACC AGCGCCGACG ACAACGGTGC TGCCGACGAG 
CTAAGGAGAT CTGTCATCGA GATCAGCCGA GCACTCAAGG AGCTCTTCCA CGAAGCCAGG 
GAAAGAGCCT CCAAGGCCCT GGGCTTTGCT AAAATGCTGA GGAAGGACCT AGAAATAGCA 
GCAGAGTTCG TGCTATCTGC ATCAGCCCGA GAGCTCCTGG ACGCTCTGAA AGCAAAGCAG 
TATGTTAAGG TACAGATTCC CGGGTTAGAG AATTTGCACG TGTTTGTCCC CGACAGCCTC 
GCTGAGGAGA AGAAAATTAT TTTGCAGCTA CTCAATGCTG CCACAGGAAA GGACTGCTCA 
AAGGATCCAG ACGACGTCTT CATGGATGCC TTCCTGCTCC TGACCAAGCA TGGGGACCGA 
GCCCGTGACT CAGAAGATGG CTGGGGCACA TGGGAAGCTC GGGCTGTCAA AATTGTGCCT 
CAGGTGGAGA CTGTGGACAC CCTGAGAAGC ATGCAGGTGG ACAACCTTCT GCTGGTTGTC 
ATGGAGTCTG CTCACCTCGT ACTTCAGAGA AAAGCCTTCC AGCAGTCCAT TGAGGGGCTG 
ATGACTGTAC GCCATGAGCA GACATCTAGC CAGCCCATCA TCGCCAAAGG TTTGCAGCAG 
CTCAAGAACG ATGCACTTGA GCTATGCAAC AGAATCAGCG ATGCCATCGA CCGTGTGGAC 
CACATGTTCA CCCTGGAGTT CGATGCTGAG GTCGAGGAGT CTGAGTCGGC CACGCTGCAG 
CAGTACTACC GAGAAGCCAT GATTCAGGGC TACAACTTTG GGTTTGAGTA TCATAAAGAA 
GTTGTTCGTT TGATGTCTGG GGAATTCAGG CAGAAGATAG GAGACAAATA TATAAGCTTC 
GCCCAGAAGT GGATGAATTA CGTGCTGACC AAATGCGAGA GCGGCAGAGG CACAAGACCC 
AGATGGGCCA CCCAAGGATT TGATTTCCTA CAAGCCATTG AACCTGCCTT TATTTCAGCT 
TTACCAGAAG ATGACTTCTT GAGTTTGCAA GCCCTGATGA ATGAGTGCAT CGGGCACGTC 
ATAGGAAAGC CACACAGCCC TGTCACAGCT ATCCATCGGA ACAGCCCCCG CCCTGTGAAG 
GTGCCCCGAT GCCACAGTGA CCCTCCTAAC CCTCACCTCA TCATCCCGAC TCCAGAGGGA 
TTCAGGGGTT CCAGTGTCCC TGAAAACGAC CGCTTGGCCT CCATAGCTGC AGAACTGCAG 
TTCAGGTCTC TGAGTCGGCA CTCAAGCCCC ACGGAAGAGC GAGACGAGCC AGCGTATCCT 
CGGAGTGACT CAAGTGGATC AACTCGGAGA AGCTGGGAAC TTCGAACACT CATCAGCCAG 
ACCAAAGACT CGGCCTCTAA GCAGGGGCCC ATAGAAGCTA TCCAGAAGTC AGTCCGACTG 
TTTGAAGAGA GGAGGTATCG AGAGATGAGG AGAAAGAATA TCATCGGCCA AGTGTGCGAT 
ACCCCTAAGT CCTATGATAA CGTCATGCAT GTTGGACTGA GGAAGGTGAC ATTTAAGTGG 
CAAAGAGGAA ACAAAATTGG AGAAGGACAG TATGGAAAAG TATACACCTG CATCAGTGTT 
GACACAGGGG AGCTGATGGC CATGAAGGAG ATTCGATTTC AGCCTAACGA CCACAAGACT 
ATCAAGGAGA CTGCAGACGA GTTGAAAATA TTTGAAGGCA TCAAGCACCC CAACCTGGTC 
CGGTATTTTG GCGTGGAGCT TCACAGGGAA GAGATGTACA TCTTCATGGA GTACTGTGAT 
GAGGGTACAC TAGAGGAGGT GTCACGACTG GGCCTGCAGG AGCACGTCAT CAGGTTATAT 
ACCAAGCAGA TCACTGTCGC CATCAACGTC CTCCATGAGC ACGGCATCGT TCACCGAGAC 
ATCAAAGGTG CCAATATCTT CCTTACGTCA TCTGGACTAA TCAAGCTGGG AGATTTTGGA 
TGCTCTGTAA AACTTAAAAA CAACGCCCAG ACCATGCCCG GAGAGGTGAA CAGCACCCTA 
GGGACAGCAG CTTACATGGC CCCTGAAGTT ATTACCCGAG CCAAAGGAGA AGGCCACGGA 
CGTGCGGCAG ATATCTGGAG TCTGGGGTGC GTCGTCATAG AGATGGTGAC TGGCAAGCGG 
CCTTGGCATG AGTATGAACA CAACTTTCAG ATTATGTACA AGGTGGGGAT GGGACACAAG 
CCACCAATCC CGGAAAGGCT AAGCCCTGAA GGAAAGGCCT TTCTCTCGCA CTGCCTGGAA 
AGTGACCCGA AGATACGGTG GACAGCCAGC CAGCTCCTCG ACCACGCTTT TGTCAAGGTT 
TGCACAGATG AAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001344651.1
CDS	169..4806
Translation	MRDAIAEPVPPPALADTPAAAMEELRPAPPPQPEPDPECCPAARQECMLGESARKSMESDPEDFSDETNTETLYGTSPPSTPRQMKRLSAKHQRNSAGRPASRSNLKEKMNTPSQSPHKDLGKGVETVEEYSYKQEKKIRATLRTTERDHKKNAQCSFMLDSVAGSLPKKSIPDVDLNKPYLSLGCSNAKLPVSMPMPIARTARQTSRTDCPADRLKFFETLRLLLKLTSVSKKKDREQRGQENTAAFWFNRSNELIWLELQAWHAGRTINDQDLFLYTARQAIPDIINEILTFKVNYGSIAFSSNGAGFNGPLVEGQCRTPQETNRVGCSSYHEHLQRQRVSFEQVKRIMELLEYMEALYPSLQALQKDYERYAAKDFEDRVQALCLWLNITKDLNQKLRIMGTVLGIKNLSDIGWPVFEIPSPRPSKGYEPEDEVEDTEVELRELESGTEESDEEPTPSPRVPELRLSTDAILDSRSQGCVSRKLERLESEEDSIGWGTADCGPEASRHCLTSIYRPFVDKALKQMGLRKLILRLHKLMNGSLQRARVALVKDDRPVEFSDFPGPMWGSDYVQLSGTPPSSEQKCSAVSWEELRAMDLPSFEPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLQEVLGGLEKTDCNMDAFEEDLQKMLMVYFDYMRSWIQMLQQLPQASHSLKNLLEEEWNFTKEITHYIRGGEAQAGKLFCDIAGMLLKSTGSFLESGLQESCAELWTSADDNGAADELRRSVIEISRALKELFHEARERASKALGFAKMLRKDLEIAAEFVLSASARELLDALKAKQYVKVQIPGLENLHVFVPDSLAEEKKIILQLLNAATGKDCSKDPDDVFMDAFLLLTKHGDRARDSEDGWGTWEARAVKIVPQVETVDTLRSMQVDNLLLVVMESAHLVLQRKAFQQSIEGLMTVRHEQTSSQPIIAKGLQQLKNDALELCNRISDAIDRVDHMFTLEFDAEVEESESATLQQYYREAMIQGYNFGFEYHKEVVRLMSGEFRQKIGDKYISFAQKWMNYVLTKCESGRGTRPRWATQGFDFLQAIEPAFISALPEDDFLSLQALMNECIGHVIGKPHSPVTAIHRNSPRPVKVPRCHSDPPNPHLIIPTPEGFRGSSVPENDRLASIAAELQFRSLSRHSSPTEERDEPAYPRSDSSGSTRRSWELRTLISQTKDSASKQGPIEAIQKSVRLFEERRYREMRRKNIIGQVCDTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCISVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSRLGLQEHVIRLYTKQITVAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLKNNAQTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVVIEMVTGKRPWHEYEHNFQIMYKVGMGHKPPIPERLSPEGKAFLSHCLESDPKIRWTASQLLDHAFVKVCTDEE

Target ORF information:

RefSeq Version	NM_001357722.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mitogen-activated protein kinase kinase kinase 4 (Map3k4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001357722.1

ORF Insert Sequence:

ATGAGAGACG CCATCGCCGA GCCGGTGCCC CCTCCTGCCC TCGCCGACAC CCCTGCAGCC 
GCCATGGAGG AGCTGCGGCC AGCACCGCCG CCACAGCCCG AGCCGGATCC GGAGTGCTGC 
CCAGCGGCGA GGCAGGAGTG CATGTTGGGA GAGTCGGCTC GCAAAAGTAT GGAATCCGAT 
CCAGAGGACT TTTCTGATGA AACAAATACA GAGACTCTCT ACGGCACCTC ACCCCCAAGC 
ACACCTCGAC AGATGAAACG CCTGTCAGCC AAGCACCAGA GGAACAGCGC AGGGAGGCCG 
GCCAGCCGAT CGAACTTGAA AGAAAAAATG AACACACCGA GTCAGTCTCC ACATAAAGAT 
TTGGGGAAGG GAGTGGAGAC CGTGGAAGAA TACAGCTACA AGCAGGAGAA GAAGATTCGA 
GCAACTCTGA GAACAACGGA GCGAGACCAT AAGAAAAATG CACAGTGCTC GTTCATGTTG 
GACTCGGTGG CTGGGTCTTT GCCAAAAAAA TCGATTCCAG ATGTGGATCT CAATAAGCCT 
TACCTCAGTC TCGGCTGTAG CAATGCCAAG CTGCCCGTCT CGATGCCCAT GCCGATAGCC 
AGAACTGCAC GGCAGACTTC CCGGACTGAC TGCCCCGCAG ATCGCTTAAA GTTCTTTGAA 
ACACTGCGCC TTTTGCTAAA GCTTACCTCA GTCTCGAAGA AGAAGGACAG GGAGCAGAGG 
GGACAAGAAA ACACGGCTGC TTTCTGGTTC AACCGATCGA ACGAACTGAT CTGGTTAGAA 
CTGCAGGCCT GGCACGCGGG CCGCACCATC AATGACCAGG ACCTCTTTCT CTACACAGCC 
CGCCAGGCCA TCCCAGACAT CATCAATGAG ATCCTCACCT TCAAAGTTAA CTACGGGAGC 
ATTGCCTTCT CCAGCAATGG AGCCGGTTTC AACGGGCCCT TGGTAGAAGG CCAGTGCAGA 
ACCCCTCAGG AGACAAACCG TGTGGGCTGC TCATCGTACC ACGAGCACCT CCAGCGCCAG 
AGGGTCTCGT TTGAGCAGGT GAAGCGGATA ATGGAGCTGC TGGAGTACAT GGAGGCACTT 
TACCCATCCT TGCAGGCTCT GCAGAAGGAC TATGAACGGT ACGCCGCCAA GGACTTTGAG 
GACAGAGTGC AGGCGCTCTG CCTGTGGCTC AACATCACGA AAGATCTAAA TCAGAAGCTG 
CGGATCATGG GCACCGTGCT GGGCATCAAG AACCTATCAG ACATTGGCTG GCCAGTGTTT 
GAAATCCCCT CCCCTCGGCC GTCCAAGGGC TACGAGCCAG AGGACGAGGT CGAGGACACG 
GAGGTTGAGC TGAGGGAGCT GGAGAGCGGG ACGGAGGAGA GTGACGAGGA GCCAACCCCC 
AGTCCGAGGG TGCCAGAGCT CAGGCTGTCC ACAGACGCCA TCTTGGACAG TCGCTCCCAG 
GGCTGCGTCT CCAGGAAGCT GGAGAGGCTC GAGTCAGAGG AAGATTCCAT AGGCTGGGGG 
ACAGCGGACT GTGGCCCTGA AGCCAGCAGG CATTGTTTGA CTTCTATCTA TAGACCATTC 
GTGGACAAAG CACTGAAGCA AATGGGGCTA AGAAAGTTAA TTTTACGACT TCATAAGCTT 
ATGAATGGGT CCTTGCAAAG AGCTCGTGTA GCTCTGGTGA AGGACGACCG TCCAGTGGAG 
TTCTCTGACT TTCCAGGTCC CATGTGGGGC TCGGATTATG TGCAGTTGTC GGGAACACCT 
CCTTCCTCAG AGCAGAAGTG TAGCGCTGTG TCCTGGGAAG AACTGAGAGC CATGGACCTG 
CCTTCCTTTG AGCCCGCCTT CCTGGTGCTC TGTCGGGTCC TGCTGAACGT GATCCACGAG 
TGCCTGAAGC TGCGGCTGGA ACAGAGGCCT GCCGGGGAGC CTTCCCTCTT GAGTATCAAA 
CAGCTAGTGC GAGAGTGTAA AGAGGTCCTA AAGGGCGGGC TCCTGATGAA GCAGTATTAC 
CAGTTCATGC TGCAGGAGGT CCTGGGCGGA CTGGAGAAGA CCGACTGCAA CATGGATGCC 
TTTGAGGAGG ACCTGCAGAA GATGCTGATG GTGTATTTTG ATTACATGAG AAGCTGGATC 
CAAATGCTAC AGCAGTTACC TCAGGCTTCC CATAGCTTAA AAAACCTGCT AGAAGAGGAA 
TGGAATTTCA CCAAAGAAAT AACCCATTAT ATCCGTGGCG GAGAAGCGCA GGCTGGAAAG 
CTTTTCTGTG ACATCGCAGG GATGCTGCTG AAATCCACAG GGAGCTTTCT GGAATCCGGC 
CTGCAGGAGA GCTGTGCTGA GCTGTGGACC AGCGCCGACG ACAACGGTGC TGCCGACGAG 
CTAAGGAGAT CTGTCATCGA GATCAGCCGA GCACTCAAGG AGCTCTTCCA CGAAGCCAGG 
GAAAGAGCCT CCAAGGCCCT GGGCTTTGCT AAAATGCTGA GGAAGGACCT AGAAATAGCA 
GCAGAGTTCG TGCTATCTGC ATCAGCCCGA GAGCTCCTGG ACGCTCTGAA AGCAAAGCAG 
TATGTTAAGG TACAGATTCC CGGGTTAGAG AATTTGCACG TGTTTGTCCC CGACAGCCTC 
GCTGAGGAGA AGAAAATTAT TTTGCAGCTA CTCAATGCTG CCACAGGAAA GGACTGCTCA 
AAGGATCCAG ACGACGTCTT CATGGATGCC TTCCTGCTCC TGACCAAGCA TGGGGACCGA 
GCCCGTGACT CAGAAGATGG CTGGGGCACA TGGGAAGCTC GGGCTGTCAA AATTGTGCCT 
CAGGTGGAGA CTGTGGACAC CCTGAGAAGC ATGCAGGTGG ACAACCTTCT GCTGGTTGTC 
ATGGAGTCTG CTCACCTCGT ACTTCAGAGA AAAGCCTTCC AGCAGTCCAT TGAGGGGCTG 
ATGACTGTAC GCCATGAGCA GACATCTAGC CAGCCCATCA TCGCCAAAGG TTTGCAGCAG 
CTCAAGAACG ATGCACTTGA GCTATGCAAC AGAATCAGCG ATGCCATCGA CCGTGTGGAC 
CACATGTTCA CCCTGGAGTT CGATGCTGAG GTCGAGGAGT CTGAGTCGGC CACGCTGCAG 
CAGTACTACC GAGAAGCCAT GATTCAGGGC TACAACTTTG GGTTTGAGTA TCATAAAGAA 
GTTGTTCGTT TGATGTCTGG GGAATTCAGG CAGAAGATAG GAGACAAATA TATAAGCTTC 
GCCCAGAAGT GGATGAATTA CGTGCTGACC AAATGCGAGA GCGGCAGAGG CACAAGACCC 
AGATGGGCCA CCCAAGGATT TGATTTCCTA CAAGCCATTG AACCTGCCTT TATTTCAGCT 
TTACCAGAAG ATGACTTCTT GAGTTTGCAA GCCCTGATGA ATGAGTGCAT CGGGCACGTC 
ATAGGAAAGC CACACAGCCC TGTCACAGCT ATCCATCGGA ACAGCCCCCG CCCTGTGAAG 
GTGCCCCGAT GCCACAGTGA CCCTCCTAAC CCTCACCTCA TCATCCCGAC TCCAGAGGGA 
TTCAGGGGTT CCAGTGTCCC TGAAAACGAC CGCTTGGCCT CCATAGCTGC AGAACTGCAG 
TTCAGGTCTC TGAGTCGGCA CTCAAGCCCC ACGGAAGAGC GAGACGAGCC AGCGTATCCT 
CGGAGTGACT CAAGTGGATC AACTCGGAGA AGCTGGGAAC TTCGAACACT CATCAGCCAG 
ACCAAAGACT CGGCCTCTAA GCAGGGGCCC ATAGAAGCTA TCCAGAAGTC AGTCCGACTG 
TTTGAAGAGA GGAGGTATCG AGAGATGAGG AGAAAGAATA TCATCGGCCA AGTGTGCGAT 
ACCCCTAAGT CCTATGATAA CGTCATGCAT GTTGGACTGA GGAAGGTGAC ATTTAAGTGG 
CAAAGAGGAA ACAAAATTGG AGAAGGACAG TATGGAAAAG TATACACCTG CATCAGTGTT 
GACACAGGGG AGCTGATGGC CATGAAGGAG ATTCGATTTC AGCCTAACGA CCACAAGACT 
ATCAAGGAGA CTGCAGACGA GTTGAAAATA TTTGAAGGCA TCAAGCACCC CAACCTGGTC 
CGGTATTTTG GCGTGGAGCT TCACAGGGAA GAGATGTACA TCTTCATGGA GTACTGTGAT 
GAGGGTACAC TAGAGGAGGT GTCACGACTG GGCCTGCAGG AGCACGTCAT CAGGTTATAT 
ACCAAGCAGA TCACTGTCGC CATCAACGTC CTCCATGAGC ACGGCATCGT TCACCGAGAC 
ATCAAAGGTG CCAATATCTT CCTTACGTCA TCTGGACTAA TCAAGCTGGG AGATTTTGGA 
TGCTCTGTAA AACTTAAAAA CAACGCCCAG ACCATGCCCG GAGAGGTGAA CAGCACCCTA 
GGGACAGCAG CTTACATGGC CCCTGAAGTT ATTACCCGAG CCAAAGGAGA AGGCCACGGA 
CGTGCGGCAG ATATCTGGAG TCTGGGGTGC GTCGTCATAG AGATGGTGAC TGGCAAGCGG 
CCTTGGCATG AGTATGAACA CAACTTTCAG ATTATGTACA AGGTGGGGAT GGGACACAAG 
CCACCAATCC CGGAAAGGCT AAGCCCTGAA GGAAAGGCCT TTCTCTCGCA CTGCCTGGAA 
AGTGACCCGA AGATACGGTG GACAGCCAGC CAGCTCCTCG ACCACGCTTT TGTCAAGGTT 
TGCACAGATG AAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K4 ( XM_006523329.3 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_006523392.1 Mus musculus mitogen-activated protein kinase kinase kinase 4 (Map3k4), transcript variant X4, mRNA.

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CloneID	OMu52902
Clone ID Related Accession (Same CDS sequence)	NM_001357722.1 , XM_006523329.3
Accession Version	XM_006523329.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4638bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	mitogen-activated protein kinase kinase kinase 4 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006523329.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGACG CCATCGCCGA GCCGGTGCCC CCTCCTGCCC TCGCCGACAC CCCTGCAGCC 
GCCATGGAGG AGCTGCGGCC AGCACCGCCG CCACAGCCCG AGCCGGATCC GGAGTGCTGC 
CCAGCGGCGA GGCAGGAGTG CATGTTGGGA GAGTCGGCTC GCAAAAGTAT GGAATCCGAT 
CCAGAGGACT TTTCTGATGA AACAAATACA GAGACTCTCT ACGGCACCTC ACCCCCAAGC 
ACACCTCGAC AGATGAAACG CCTGTCAGCC AAGCACCAGA GGAACAGCGC AGGGAGGCCG 
GCCAGCCGAT CGAACTTGAA AGAAAAAATG AACACACCGA GTCAGTCTCC ACATAAAGAT 
TTGGGGAAGG GAGTGGAGAC CGTGGAAGAA TACAGCTACA AGCAGGAGAA GAAGATTCGA 
GCAACTCTGA GAACAACGGA GCGAGACCAT AAGAAAAATG CACAGTGCTC GTTCATGTTG 
GACTCGGTGG CTGGGTCTTT GCCAAAAAAA TCGATTCCAG ATGTGGATCT CAATAAGCCT 
TACCTCAGTC TCGGCTGTAG CAATGCCAAG CTGCCCGTCT CGATGCCCAT GCCGATAGCC 
AGAACTGCAC GGCAGACTTC CCGGACTGAC TGCCCCGCAG ATCGCTTAAA GTTCTTTGAA 
ACACTGCGCC TTTTGCTAAA GCTTACCTCA GTCTCGAAGA AGAAGGACAG GGAGCAGAGG 
GGACAAGAAA ACACGGCTGC TTTCTGGTTC AACCGATCGA ACGAACTGAT CTGGTTAGAA 
CTGCAGGCCT GGCACGCGGG CCGCACCATC AATGACCAGG ACCTCTTTCT CTACACAGCC 
CGCCAGGCCA TCCCAGACAT CATCAATGAG ATCCTCACCT TCAAAGTTAA CTACGGGAGC 
ATTGCCTTCT CCAGCAATGG AGCCGGTTTC AACGGGCCCT TGGTAGAAGG CCAGTGCAGA 
ACCCCTCAGG AGACAAACCG TGTGGGCTGC TCATCGTACC ACGAGCACCT CCAGCGCCAG 
AGGGTCTCGT TTGAGCAGGT GAAGCGGATA ATGGAGCTGC TGGAGTACAT GGAGGCACTT 
TACCCATCCT TGCAGGCTCT GCAGAAGGAC TATGAACGGT ACGCCGCCAA GGACTTTGAG 
GACAGAGTGC AGGCGCTCTG CCTGTGGCTC AACATCACGA AAGATCTAAA TCAGAAGCTG 
CGGATCATGG GCACCGTGCT GGGCATCAAG AACCTATCAG ACATTGGCTG GCCAGTGTTT 
GAAATCCCCT CCCCTCGGCC GTCCAAGGGC TACGAGCCAG AGGACGAGGT CGAGGACACG 
GAGGTTGAGC TGAGGGAGCT GGAGAGCGGG ACGGAGGAGA GTGACGAGGA GCCAACCCCC 
AGTCCGAGGG TGCCAGAGCT CAGGCTGTCC ACAGACGCCA TCTTGGACAG TCGCTCCCAG 
GGCTGCGTCT CCAGGAAGCT GGAGAGGCTC GAGTCAGAGG AAGATTCCAT AGGCTGGGGG 
ACAGCGGACT GTGGCCCTGA AGCCAGCAGG CATTGTTTGA CTTCTATCTA TAGACCATTC 
GTGGACAAAG CACTGAAGCA AATGGGGCTA AGAAAGTTAA TTTTACGACT TCATAAGCTT 
ATGAATGGGT CCTTGCAAAG AGCTCGTGTA GCTCTGGTGA AGGACGACCG TCCAGTGGAG 
TTCTCTGACT TTCCAGGTCC CATGTGGGGC TCGGATTATG TGCAGTTGTC GGGAACACCT 
CCTTCCTCAG AGCAGAAGTG TAGCGCTGTG TCCTGGGAAG AACTGAGAGC CATGGACCTG 
CCTTCCTTTG AGCCCGCCTT CCTGGTGCTC TGTCGGGTCC TGCTGAACGT GATCCACGAG 
TGCCTGAAGC TGCGGCTGGA ACAGAGGCCT GCCGGGGAGC CTTCCCTCTT GAGTATCAAA 
CAGCTAGTGC GAGAGTGTAA AGAGGTCCTA AAGGGCGGGC TCCTGATGAA GCAGTATTAC 
CAGTTCATGC TGCAGGAGGT CCTGGGCGGA CTGGAGAAGA CCGACTGCAA CATGGATGCC 
TTTGAGGAGG ACCTGCAGAA GATGCTGATG GTGTATTTTG ATTACATGAG AAGCTGGATC 
CAAATGCTAC AGCAGTTACC TCAGGCTTCC CATAGCTTAA AAAACCTGCT AGAAGAGGAA 
TGGAATTTCA CCAAAGAAAT AACCCATTAT ATCCGTGGCG GAGAAGCGCA GGCTGGAAAG 
CTTTTCTGTG ACATCGCAGG GATGCTGCTG AAATCCACAG GGAGCTTTCT GGAATCCGGC 
CTGCAGGAGA GCTGTGCTGA GCTGTGGACC AGCGCCGACG ACAACGGTGC TGCCGACGAG 
CTAAGGAGAT CTGTCATCGA GATCAGCCGA GCACTCAAGG AGCTCTTCCA CGAAGCCAGG 
GAAAGAGCCT CCAAGGCCCT GGGCTTTGCT AAAATGCTGA GGAAGGACCT AGAAATAGCA 
GCAGAGTTCG TGCTATCTGC ATCAGCCCGA GAGCTCCTGG ACGCTCTGAA AGCAAAGCAG 
TATGTTAAGG TACAGATTCC CGGGTTAGAG AATTTGCACG TGTTTGTCCC CGACAGCCTC 
GCTGAGGAGA AGAAAATTAT TTTGCAGCTA CTCAATGCTG CCACAGGAAA GGACTGCTCA 
AAGGATCCAG ACGACGTCTT CATGGATGCC TTCCTGCTCC TGACCAAGCA TGGGGACCGA 
GCCCGTGACT CAGAAGATGG CTGGGGCACA TGGGAAGCTC GGGCTGTCAA AATTGTGCCT 
CAGGTGGAGA CTGTGGACAC CCTGAGAAGC ATGCAGGTGG ACAACCTTCT GCTGGTTGTC 
ATGGAGTCTG CTCACCTCGT ACTTCAGAGA AAAGCCTTCC AGCAGTCCAT TGAGGGGCTG 
ATGACTGTAC GCCATGAGCA GACATCTAGC CAGCCCATCA TCGCCAAAGG TTTGCAGCAG 
CTCAAGAACG ATGCACTTGA GCTATGCAAC AGAATCAGCG ATGCCATCGA CCGTGTGGAC 
CACATGTTCA CCCTGGAGTT CGATGCTGAG GTCGAGGAGT CTGAGTCGGC CACGCTGCAG 
CAGTACTACC GAGAAGCCAT GATTCAGGGC TACAACTTTG GGTTTGAGTA TCATAAAGAA 
GTTGTTCGTT TGATGTCTGG GGAATTCAGG CAGAAGATAG GAGACAAATA TATAAGCTTC 
GCCCAGAAGT GGATGAATTA CGTGCTGACC AAATGCGAGA GCGGCAGAGG CACAAGACCC 
AGATGGGCCA CCCAAGGATT TGATTTCCTA CAAGCCATTG AACCTGCCTT TATTTCAGCT 
TTACCAGAAG ATGACTTCTT GAGTTTGCAA GCCCTGATGA ATGAGTGCAT CGGGCACGTC 
ATAGGAAAGC CACACAGCCC TGTCACAGCT ATCCATCGGA ACAGCCCCCG CCCTGTGAAG 
GTGCCCCGAT GCCACAGTGA CCCTCCTAAC CCTCACCTCA TCATCCCGAC TCCAGAGGGA 
TTCAGGGGTT CCAGTGTCCC TGAAAACGAC CGCTTGGCCT CCATAGCTGC AGAACTGCAG 
TTCAGGTCTC TGAGTCGGCA CTCAAGCCCC ACGGAAGAGC GAGACGAGCC AGCGTATCCT 
CGGAGTGACT CAAGTGGATC AACTCGGAGA AGCTGGGAAC TTCGAACACT CATCAGCCAG 
ACCAAAGACT CGGCCTCTAA GCAGGGGCCC ATAGAAGCTA TCCAGAAGTC AGTCCGACTG 
TTTGAAGAGA GGAGGTATCG AGAGATGAGG AGAAAGAATA TCATCGGCCA AGTGTGCGAT 
ACCCCTAAGT CCTATGATAA CGTCATGCAT GTTGGACTGA GGAAGGTGAC ATTTAAGTGG 
CAAAGAGGAA ACAAAATTGG AGAAGGACAG TATGGAAAAG TATACACCTG CATCAGTGTT 
GACACAGGGG AGCTGATGGC CATGAAGGAG ATTCGATTTC AGCCTAACGA CCACAAGACT 
ATCAAGGAGA CTGCAGACGA GTTGAAAATA TTTGAAGGCA TCAAGCACCC CAACCTGGTC 
CGGTATTTTG GCGTGGAGCT TCACAGGGAA GAGATGTACA TCTTCATGGA GTACTGTGAT 
GAGGGTACAC TAGAGGAGGT GTCACGACTG GGCCTGCAGG AGCACGTCAT CAGGTTATAT 
ACCAAGCAGA TCACTGTCGC CATCAACGTC CTCCATGAGC ACGGCATCGT TCACCGAGAC 
ATCAAAGGTG CCAATATCTT CCTTACGTCA TCTGGACTAA TCAAGCTGGG AGATTTTGGA 
TGCTCTGTAA AACTTAAAAA CAACGCCCAG ACCATGCCCG GAGAGGTGAA CAGCACCCTA 
GGGACAGCAG CTTACATGGC CCCTGAAGTT ATTACCCGAG CCAAAGGAGA AGGCCACGGA 
CGTGCGGCAG ATATCTGGAG TCTGGGGTGC GTCGTCATAG AGATGGTGAC TGGCAAGCGG 
CCTTGGCATG AGTATGAACA CAACTTTCAG ATTATGTACA AGGTGGGGAT GGGACACAAG 
CCACCAATCC CGGAAAGGCT AAGCCCTGAA GGAAAGGCCT TTCTCTCGCA CTGCCTGGAA 
AGTGACCCGA AGATACGGTG GACAGCCAGC CAGCTCCTCG ACCACGCTTT TGTCAAGGTT 
TGCACAGATG AAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523392.1
CDS	169..4806
Translation	MRDAIAEPVPPPALADTPAAAMEELRPAPPPQPEPDPECCPAARQECMLGESARKSMESDPEDFSDETNTETLYGTSPPSTPRQMKRLSAKHQRNSAGRPASRSNLKEKMNTPSQSPHKDLGKGVETVEEYSYKQEKKIRATLRTTERDHKKNAQCSFMLDSVAGSLPKKSIPDVDLNKPYLSLGCSNAKLPVSMPMPIARTARQTSRTDCPADRLKFFETLRLLLKLTSVSKKKDREQRGQENTAAFWFNRSNELIWLELQAWHAGRTINDQDLFLYTARQAIPDIINEILTFKVNYGSIAFSSNGAGFNGPLVEGQCRTPQETNRVGCSSYHEHLQRQRVSFEQVKRIMELLEYMEALYPSLQALQKDYERYAAKDFEDRVQALCLWLNITKDLNQKLRIMGTVLGIKNLSDIGWPVFEIPSPRPSKGYEPEDEVEDTEVELRELESGTEESDEEPTPSPRVPELRLSTDAILDSRSQGCVSRKLERLESEEDSIGWGTADCGPEASRHCLTSIYRPFVDKALKQMGLRKLILRLHKLMNGSLQRARVALVKDDRPVEFSDFPGPMWGSDYVQLSGTPPSSEQKCSAVSWEELRAMDLPSFEPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLQEVLGGLEKTDCNMDAFEEDLQKMLMVYFDYMRSWIQMLQQLPQASHSLKNLLEEEWNFTKEITHYIRGGEAQAGKLFCDIAGMLLKSTGSFLESGLQESCAELWTSADDNGAADELRRSVIEISRALKELFHEARERASKALGFAKMLRKDLEIAAEFVLSASARELLDALKAKQYVKVQIPGLENLHVFVPDSLAEEKKIILQLLNAATGKDCSKDPDDVFMDAFLLLTKHGDRARDSEDGWGTWEARAVKIVPQVETVDTLRSMQVDNLLLVVMESAHLVLQRKAFQQSIEGLMTVRHEQTSSQPIIAKGLQQLKNDALELCNRISDAIDRVDHMFTLEFDAEVEESESATLQQYYREAMIQGYNFGFEYHKEVVRLMSGEFRQKIGDKYISFAQKWMNYVLTKCESGRGTRPRWATQGFDFLQAIEPAFISALPEDDFLSLQALMNECIGHVIGKPHSPVTAIHRNSPRPVKVPRCHSDPPNPHLIIPTPEGFRGSSVPENDRLASIAAELQFRSLSRHSSPTEERDEPAYPRSDSSGSTRRSWELRTLISQTKDSASKQGPIEAIQKSVRLFEERRYREMRRKNIIGQVCDTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCISVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSRLGLQEHVIRLYTKQITVAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLKNNAQTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVVIEMVTGKRPWHEYEHNFQIMYKVGMGHKPPIPERLSPEGKAFLSHCLESDPKIRWTASQLLDHAFVKVCTDEE

Target ORF information:

RefSeq Version	XM_006523329.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus mitogen-activated protein kinase kinase kinase 4 (Map3k4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523329.3

ORF Insert Sequence:

ATGAGAGACG CCATCGCCGA GCCGGTGCCC CCTCCTGCCC TCGCCGACAC CCCTGCAGCC 
GCCATGGAGG AGCTGCGGCC AGCACCGCCG CCACAGCCCG AGCCGGATCC GGAGTGCTGC 
CCAGCGGCGA GGCAGGAGTG CATGTTGGGA GAGTCGGCTC GCAAAAGTAT GGAATCCGAT 
CCAGAGGACT TTTCTGATGA AACAAATACA GAGACTCTCT ACGGCACCTC ACCCCCAAGC 
ACACCTCGAC AGATGAAACG CCTGTCAGCC AAGCACCAGA GGAACAGCGC AGGGAGGCCG 
GCCAGCCGAT CGAACTTGAA AGAAAAAATG AACACACCGA GTCAGTCTCC ACATAAAGAT 
TTGGGGAAGG GAGTGGAGAC CGTGGAAGAA TACAGCTACA AGCAGGAGAA GAAGATTCGA 
GCAACTCTGA GAACAACGGA GCGAGACCAT AAGAAAAATG CACAGTGCTC GTTCATGTTG 
GACTCGGTGG CTGGGTCTTT GCCAAAAAAA TCGATTCCAG ATGTGGATCT CAATAAGCCT 
TACCTCAGTC TCGGCTGTAG CAATGCCAAG CTGCCCGTCT CGATGCCCAT GCCGATAGCC 
AGAACTGCAC GGCAGACTTC CCGGACTGAC TGCCCCGCAG ATCGCTTAAA GTTCTTTGAA 
ACACTGCGCC TTTTGCTAAA GCTTACCTCA GTCTCGAAGA AGAAGGACAG GGAGCAGAGG 
GGACAAGAAA ACACGGCTGC TTTCTGGTTC AACCGATCGA ACGAACTGAT CTGGTTAGAA 
CTGCAGGCCT GGCACGCGGG CCGCACCATC AATGACCAGG ACCTCTTTCT CTACACAGCC 
CGCCAGGCCA TCCCAGACAT CATCAATGAG ATCCTCACCT TCAAAGTTAA CTACGGGAGC 
ATTGCCTTCT CCAGCAATGG AGCCGGTTTC AACGGGCCCT TGGTAGAAGG CCAGTGCAGA 
ACCCCTCAGG AGACAAACCG TGTGGGCTGC TCATCGTACC ACGAGCACCT CCAGCGCCAG 
AGGGTCTCGT TTGAGCAGGT GAAGCGGATA ATGGAGCTGC TGGAGTACAT GGAGGCACTT 
TACCCATCCT TGCAGGCTCT GCAGAAGGAC TATGAACGGT ACGCCGCCAA GGACTTTGAG 
GACAGAGTGC AGGCGCTCTG CCTGTGGCTC AACATCACGA AAGATCTAAA TCAGAAGCTG 
CGGATCATGG GCACCGTGCT GGGCATCAAG AACCTATCAG ACATTGGCTG GCCAGTGTTT 
GAAATCCCCT CCCCTCGGCC GTCCAAGGGC TACGAGCCAG AGGACGAGGT CGAGGACACG 
GAGGTTGAGC TGAGGGAGCT GGAGAGCGGG ACGGAGGAGA GTGACGAGGA GCCAACCCCC 
AGTCCGAGGG TGCCAGAGCT CAGGCTGTCC ACAGACGCCA TCTTGGACAG TCGCTCCCAG 
GGCTGCGTCT CCAGGAAGCT GGAGAGGCTC GAGTCAGAGG AAGATTCCAT AGGCTGGGGG 
ACAGCGGACT GTGGCCCTGA AGCCAGCAGG CATTGTTTGA CTTCTATCTA TAGACCATTC 
GTGGACAAAG CACTGAAGCA AATGGGGCTA AGAAAGTTAA TTTTACGACT TCATAAGCTT 
ATGAATGGGT CCTTGCAAAG AGCTCGTGTA GCTCTGGTGA AGGACGACCG TCCAGTGGAG 
TTCTCTGACT TTCCAGGTCC CATGTGGGGC TCGGATTATG TGCAGTTGTC GGGAACACCT 
CCTTCCTCAG AGCAGAAGTG TAGCGCTGTG TCCTGGGAAG AACTGAGAGC CATGGACCTG 
CCTTCCTTTG AGCCCGCCTT CCTGGTGCTC TGTCGGGTCC TGCTGAACGT GATCCACGAG 
TGCCTGAAGC TGCGGCTGGA ACAGAGGCCT GCCGGGGAGC CTTCCCTCTT GAGTATCAAA 
CAGCTAGTGC GAGAGTGTAA AGAGGTCCTA AAGGGCGGGC TCCTGATGAA GCAGTATTAC 
CAGTTCATGC TGCAGGAGGT CCTGGGCGGA CTGGAGAAGA CCGACTGCAA CATGGATGCC 
TTTGAGGAGG ACCTGCAGAA GATGCTGATG GTGTATTTTG ATTACATGAG AAGCTGGATC 
CAAATGCTAC AGCAGTTACC TCAGGCTTCC CATAGCTTAA AAAACCTGCT AGAAGAGGAA 
TGGAATTTCA CCAAAGAAAT AACCCATTAT ATCCGTGGCG GAGAAGCGCA GGCTGGAAAG 
CTTTTCTGTG ACATCGCAGG GATGCTGCTG AAATCCACAG GGAGCTTTCT GGAATCCGGC 
CTGCAGGAGA GCTGTGCTGA GCTGTGGACC AGCGCCGACG ACAACGGTGC TGCCGACGAG 
CTAAGGAGAT CTGTCATCGA GATCAGCCGA GCACTCAAGG AGCTCTTCCA CGAAGCCAGG 
GAAAGAGCCT CCAAGGCCCT GGGCTTTGCT AAAATGCTGA GGAAGGACCT AGAAATAGCA 
GCAGAGTTCG TGCTATCTGC ATCAGCCCGA GAGCTCCTGG ACGCTCTGAA AGCAAAGCAG 
TATGTTAAGG TACAGATTCC CGGGTTAGAG AATTTGCACG TGTTTGTCCC CGACAGCCTC 
GCTGAGGAGA AGAAAATTAT TTTGCAGCTA CTCAATGCTG CCACAGGAAA GGACTGCTCA 
AAGGATCCAG ACGACGTCTT CATGGATGCC TTCCTGCTCC TGACCAAGCA TGGGGACCGA 
GCCCGTGACT CAGAAGATGG CTGGGGCACA TGGGAAGCTC GGGCTGTCAA AATTGTGCCT 
CAGGTGGAGA CTGTGGACAC CCTGAGAAGC ATGCAGGTGG ACAACCTTCT GCTGGTTGTC 
ATGGAGTCTG CTCACCTCGT ACTTCAGAGA AAAGCCTTCC AGCAGTCCAT TGAGGGGCTG 
ATGACTGTAC GCCATGAGCA GACATCTAGC CAGCCCATCA TCGCCAAAGG TTTGCAGCAG 
CTCAAGAACG ATGCACTTGA GCTATGCAAC AGAATCAGCG ATGCCATCGA CCGTGTGGAC 
CACATGTTCA CCCTGGAGTT CGATGCTGAG GTCGAGGAGT CTGAGTCGGC CACGCTGCAG 
CAGTACTACC GAGAAGCCAT GATTCAGGGC TACAACTTTG GGTTTGAGTA TCATAAAGAA 
GTTGTTCGTT TGATGTCTGG GGAATTCAGG CAGAAGATAG GAGACAAATA TATAAGCTTC 
GCCCAGAAGT GGATGAATTA CGTGCTGACC AAATGCGAGA GCGGCAGAGG CACAAGACCC 
AGATGGGCCA CCCAAGGATT TGATTTCCTA CAAGCCATTG AACCTGCCTT TATTTCAGCT 
TTACCAGAAG ATGACTTCTT GAGTTTGCAA GCCCTGATGA ATGAGTGCAT CGGGCACGTC 
ATAGGAAAGC CACACAGCCC TGTCACAGCT ATCCATCGGA ACAGCCCCCG CCCTGTGAAG 
GTGCCCCGAT GCCACAGTGA CCCTCCTAAC CCTCACCTCA TCATCCCGAC TCCAGAGGGA 
TTCAGGGGTT CCAGTGTCCC TGAAAACGAC CGCTTGGCCT CCATAGCTGC AGAACTGCAG 
TTCAGGTCTC TGAGTCGGCA CTCAAGCCCC ACGGAAGAGC GAGACGAGCC AGCGTATCCT 
CGGAGTGACT CAAGTGGATC AACTCGGAGA AGCTGGGAAC TTCGAACACT CATCAGCCAG 
ACCAAAGACT CGGCCTCTAA GCAGGGGCCC ATAGAAGCTA TCCAGAAGTC AGTCCGACTG 
TTTGAAGAGA GGAGGTATCG AGAGATGAGG AGAAAGAATA TCATCGGCCA AGTGTGCGAT 
ACCCCTAAGT CCTATGATAA CGTCATGCAT GTTGGACTGA GGAAGGTGAC ATTTAAGTGG 
CAAAGAGGAA ACAAAATTGG AGAAGGACAG TATGGAAAAG TATACACCTG CATCAGTGTT 
GACACAGGGG AGCTGATGGC CATGAAGGAG ATTCGATTTC AGCCTAACGA CCACAAGACT 
ATCAAGGAGA CTGCAGACGA GTTGAAAATA TTTGAAGGCA TCAAGCACCC CAACCTGGTC 
CGGTATTTTG GCGTGGAGCT TCACAGGGAA GAGATGTACA TCTTCATGGA GTACTGTGAT 
GAGGGTACAC TAGAGGAGGT GTCACGACTG GGCCTGCAGG AGCACGTCAT CAGGTTATAT 
ACCAAGCAGA TCACTGTCGC CATCAACGTC CTCCATGAGC ACGGCATCGT TCACCGAGAC 
ATCAAAGGTG CCAATATCTT CCTTACGTCA TCTGGACTAA TCAAGCTGGG AGATTTTGGA 
TGCTCTGTAA AACTTAAAAA CAACGCCCAG ACCATGCCCG GAGAGGTGAA CAGCACCCTA 
GGGACAGCAG CTTACATGGC CCCTGAAGTT ATTACCCGAG CCAAAGGAGA AGGCCACGGA 
CGTGCGGCAG ATATCTGGAG TCTGGGGTGC GTCGTCATAG AGATGGTGAC TGGCAAGCGG 
CCTTGGCATG AGTATGAACA CAACTTTCAG ATTATGTACA AGGTGGGGAT GGGACACAAG 
CCACCAATCC CGGAAAGGCT AAGCCCTGAA GGAAAGGCCT TTCTCTCGCA CTGCCTGGAA 
AGTGACCCGA AGATACGGTG GACAGCCAGC CAGCTCCTCG ACCACGCTTT TGTCAAGGTT 
TGCACAGATG AAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MAP3K4 Controls the Chromatin Modifier HDAC6 during Trophoblast Stem Cell Epithelial-to-Mesenchymal Transition.
Cell reports18(10)2387-2400(2017 Mar)
Mobley RJ,Raghu D,Duke LD,Abell-Hart K,Zawistowski JS,Lutz K,Gomez SM,Roy S,Homayouni R,Johnson GL,Abell AN

Gadd45a Protein Promotes Skeletal Muscle Atrophy by Forming a Complex with the Protein Kinase MEKK4.
The Journal of biological chemistry291(34)17496-17509(2016 08)
Bullard SA,Seo S,Schilling B,Dyle MC,Dierdorff JM,Ebert SM,DeLau AD,Gibson BW,Adams CM

Genetic Analyses Reveal Functions for MAP2K3 and MAP2K6 in Mouse Testis Determination.
Biology of reproduction94(5)103(2016 05)
Warr N,Siggers P,Carr? GA,Wells S,Greenfield A

MEKK4 Signaling Regulates Sensory Cell Development and Function in the Mouse Inner Ear.
The Journal of neuroscience : the official journal of the Society for Neuroscience36(4)1347-61(2016 Jan)
Haque K,Pandey AK,Zheng HW,Riazuddin S,Sha SH,Puligilla C

Transgenic expression of Map3k4 rescues T-associated sex reversal (Tas) in mice.
Human molecular genetics23(11)3035-44(2014 Jun)
Warr N,Siggers P,Carr? GA,Bogani D,Brixey R,Akiyoshi M,Tachibana M,Teboul L,Wells S,Sanderson J,Greenfield A

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MAP3K4 cDNA ORF clone, Mus musculus(House mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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