Dapp1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dapp1
Entrez Gene ID	26377
Full Name	dual adaptor for phosphotyrosine and 3-phosphoinositides 1
Synonyms	Bam32
General protein information	Preferred Names dual adaptor for phosphotyrosine and 3-phosphoinositides 1 Names dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide B-cell adapter molecule of 32 kDa b lymphocyte adapter protein Bam32
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 3 Map Location: 3\|3 G3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001310753.1	NP_001297682.1	dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide isoform 2
XM_017319585.1	XP_017175074.1	dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide isoform X1
NM_011932.3	NP_036062.1	dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide isoform 1
XM_017319585.2	XP_017175074.1	dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	DAPP1	XP_517361.2
Canis lupus familiaris (dog)	DAPP1	XP_535669.2
Bos taurus (cattle)	DAPP1	NP_001070359.1
Gallus gallus (chicken)	DAPP1	NP_001012951.2
Danio rerio (zebrafish)	dapp1	NP_001017691.1
Xenopus tropicalis (tropical clawed frog)	dapp1	NP_001015723.1
Mus musculus (house mouse)	Dapp1	NP_036062.1
Homo sapiens (human)	DAPP1	NP_055210.2
	DAPP1	XP_001107831.2
Rattus norvegicus (Norway rat)	Dapp1	NP_001102038.1

Gene Ontology
Bibliography

Related articles in PubMed

The B cell adaptor molecule Bam32 is critically important for optimal antibody response and resistance to Trypanosoma congolense infection in mice.
Onyilagha C, Jia P, Jayachandran N, Hou S, Okwor I, Kuriakose S, Marshall A, Uzonna JE
PLoS neglected tropical diseases9(4)e0003716(2015 Apr)

LAT-independent Erk activation via Bam32-PLC-?1-Pak1 complexes: GTPase-independent Pak1 activation.
Rouquette-Jazdanian AK, Sommers CL, Kortum RL, Morrison DK, Samelson LE
Molecular cell48(2)298-312(2012 Oct)

The PH domain adaptor protein Bam32/DAPP1 functions in mast cells to restrain Fc?RI-induced calcium flux and granule release.
Hou S, Pauls SD, Liu P, Marshall AJ
Molecular immunology48(1-3)89-97(2010 Nov-Dec)

Bam32/DAPP1 promotes B cell adhesion and formation of polarized conjugates with T cells.
Al-Alwan M, Hou S, Zhang TT, Makondo K, Marshall AJ
Journal of immunology (Baltimore, Md. : 1950)184(12)6961-9(2010 Jun)

The pleckstrin homology domain adaptor protein Bam32/DAPP1 is required for germinal center progression.
Zhang TT, Al-Alwan M, Marshall AJ
Journal of immunology (Baltimore, Md. : 1950)184(1)164-72(2010 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These data show that Bam32 is important for optimal anti-trypanosome IgG antibody response and suppression of disease-promoting proinflammatory cytokines.
Title: The B cell adaptor molecule Bam32 is critically important for optimal antibody response and resistance to Trypanosoma congolense infection in mice.

our results identify Bam32 as a novel regulator of mast cell activation
Title: The PH domain adaptor protein Bam32/DAPP1 functions in mast cells to restrain Fc?RI-induced calcium flux and granule release.

Bam32 serves to integrate PI3K and Src kinase signaling to promote Rac-dependent B cell adhesive interactions important for Ag presentation function.
Title: Bam32/DAPP1 promotes B cell adhesion and formation of polarized conjugates with T cells.

Bam32 is not required for germinal center (GC) initiation, but rather functions in a late checkpoint of GC progression associated with T cell recruitment and GC B cell survival.
Title: The pleckstrin homology domain adaptor protein Bam32/DAPP1 is required for germinal center progression.

Data show that Erk activation was diminished in LAT knock-in Bam32 knockout CD4(+) T cells.
Title: Genetic evidence for the role of Erk activation in a lymphoproliferative disease of mice.

The following Dapp1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dapp1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu09181	NM_011932.3 Latest version!	Mus musculus dual adaptor for phosphotyrosine and 3-phosphoinositides 1 (Dapp1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu69196	NM_001310753.1 Latest version!	Mus musculus dual adaptor for phosphotyrosine and 3-phosphoinositides 1 (Dapp1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu80621	XM_017319585.2 Latest version!	Mus musculus dual adaptor for phosphotyrosine and 3-phosphoinositides 1 (Dapp1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OMu80621	XM_017319585.1	Mus musculus dual adaptor for phosphotyrosine and 3-phosphoinositides 1 (Dapp1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dapp1 ( NM_011932.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_036062.1 Mus musculus dual adaptor for phosphotyrosine and 3-phosphoinositides 1 (Dapp1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu09181
Clone ID Related Accession (Same CDS sequence)	NM_011932.3
Accession Version	NM_011932.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 843bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Mus musculus(house mouse)
Product	dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC146607.2, AK145745.1, AK169584.1 and BF714724.1. On Jul 18, 2015 this sequence version replaced NM_011932.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK145745.1, SRR1660815.15096.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164135, SAMN01164137 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCAGAG CAGAACTTCT AGGAGGGAAC ATGAGTACCC AGGATCCCTC AGAGCTGTGG 
GGCAGAGCAG ATGGAGGCAC AGACCTGCTC CAGGATTTGG GGTGGTATCA CGGCAACCTC 
ACCCGCCATG CTGCCGAAGC CCTTCTGCTC TCCAATGGAC GTGATGGTAG CTACCTGCTG 
CGGGACAGCA ATGAGCAGAC TGGGCTCTAC TCTCTCTCTG TGAGAGCCAA AGACTCTGTC 
AAACACTTTC ATGTTGAATA TACTGGGTAC TCGTTTAAAT TCGGCTTTAA TGAGTATTCA 
TCTCTAAAGG ATTTTGTCAA GCATTTTGCA AATCAGCCTT TGATTGGAAG CGAGACAGGG 
ACTCTGATGG TTTTGAAGCA TCCCTATCCA AGGGAAGTGG AAGAACCTTG CATTTACGAA 
TCAGTCCGGG TTCACACAGC GATGCAGACA GGAAGGACAG AGAACGACCT CGTGCCCACT 
GCGCCTTCTC TGGGCACCAA AGAAGGCTAT CTCACCAAGC AGGGAGGCCT GGTAAAGACC 
TGGAAAACAA GATGGTTTAC TTTGCAAAGG AACGAACTGA AATATTTCAA AGACCAGATG 
TCACCAGAAC CAATTCGGAT CCTAGACCTA ACAGAGTGCT CAGCTGTTCA ATTTGATTAC 
TCACAGGAAC GAGTAAACTG TTTCTGCCTA GTGTTTCCAT TCAGGACATT TTATCTCTGT 
GCAAAGACGG GAGTTGAAGC CGATGAATGG ATCAAAATAC TGAGATGGAA GTTGTCAAAA 
ATAAGAAAAC AGCTGGATCA AGGAGAAGAT ACCGTCCGAT CTAGGTCATT TATCTTCAAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036062.1
CDS	131..973
Translation	MGRAELLGGNMSTQDPSELWGRADGGTDLLQDLGWYHGNLTRHAAEALLLSNGRDGSYLLRDSNEQTGLYSLSVRAKDSVKHFHVEYTGYSFKFGFNEYSSLKDFVKHFANQPLIGSETGTLMVLKHPYPREVEEPCIYESVRVHTAMQTGRTENDLVPTAPSLGTKEGYLTKQGGLVKTWKTRWFTLQRNELKYFKDQMSPEPIRILDLTECSAVQFDYSQERVNCFCLVFPFRTFYLCAKTGVEADEWIKILRWKLSKIRKQLDQGEDTVRSRSFIFK

Target ORF information:

RefSeq Version	NM_011932.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus dual adaptor for phosphotyrosine and 3-phosphoinositides 1 (Dapp1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011932.3

ORF Insert Sequence:

ATGGGCAGAG CAGAACTTCT AGGAGGGAAC ATGAGTACCC AGGATCCCTC AGAGCTGTGG 
GGCAGAGCAG ATGGAGGCAC AGACCTGCTC CAGGATTTGG GGTGGTATCA CGGCAACCTC 
ACCCGCCATG CTGCCGAAGC CCTTCTGCTC TCCAATGGAC GTGATGGTAG CTACCTGCTG 
CGGGACAGCA ATGAGCAGAC TGGGCTCTAC TCTCTCTCTG TGAGAGCCAA AGACTCTGTC 
AAACACTTTC ATGTTGAATA TACTGGGTAC TCGTTTAAAT TCGGCTTTAA TGAGTATTCA 
TCTCTAAAGG ATTTTGTCAA GCATTTTGCA AATCAGCCTT TGATTGGAAG CGAGACAGGG 
ACTCTGATGG TTTTGAAGCA TCCCTATCCA AGGGAAGTGG AAGAACCTTG CATTTACGAA 
TCAGTCCGGG TTCACACAGC GATGCAGACA GGAAGGACAG AGAACGACCT CGTGCCCACT 
GCGCCTTCTC TGGGCACCAA AGAAGGCTAT CTCACCAAGC AGGGAGGCCT GGTAAAGACC 
TGGAAAACAA GATGGTTTAC TTTGCAAAGG AACGAACTGA AATATTTCAA AGACCAGATG 
TCACCAGAAC CAATTCGGAT CCTAGACCTA ACAGAGTGCT CAGCTGTTCA ATTTGATTAC 
TCACAGGAAC GAGTAAACTG TTTCTGCCTA GTGTTTCCAT TCAGGACATT TTATCTCTGT 
GCAAAGACGG GAGTTGAAGC CGATGAATGG ATCAAAATAC TGAGATGGAA GTTGTCAAAA 
ATAAGAAAAC AGCTGGATCA AGGAGAAGAT ACCGTCCGAT CTAGGTCATT TATCTTCAAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dapp1 ( NM_001310753.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001297682.1 Mus musculus dual adaptor for phosphotyrosine and 3-phosphoinositides 1 (Dapp1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu69196
Clone ID Related Accession (Same CDS sequence)	NM_001310753.1
Accession Version	NM_001310753.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 720bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-21
Organism	Mus musculus(house mouse)
Product	dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC146607.2, AK169584.1 and BF714724.1. On Jul 18, 2015 this sequence version replaced XM_011240126.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK169584.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGGCAGAG CAGAACTTCT AGGAGGGAAC ATGAGTACCC AGGATCCCTC AGAGCTGTGG 
GGCAGAGCAG ATGGAGGCAC AGACCTGCTC CAGGATTTGG GAGCCAAAGA CTCTGTCAAA 
CACTTTCATG TTGAATATAC TGGGTACTCG TTTAAATTCG GCTTTAATGA GTATTCATCT 
CTAAAGGATT TTGTCAAGCA TTTTGCAAAT CAGCCTTTGA TTGGAAGCGA GACAGGGACT 
CTGATGGTTT TGAAGCATCC CTATCCAAGG GAAGTGGAAG AACCTTGCAT TTACGAATCA 
GTCCGGGTTC ACACAGCGAT GCAGACAGGA AGGACAGAGA ACGACCTCGT GCCCACTGCG 
CCTTCTCTGG GCACCAAAGA AGGCTATCTC ACCAAGCAGG GAGGCCTGGT AAAGACCTGG 
AAAACAAGAT GGTTTACTTT GCAAAGGAAC GAACTGAAAT ATTTCAAAGA CCAGATGTCA 
CCAGAACCAA TTCGGATCCT AGACCTAACA GAGTGCTCAG CTGTTCAATT TGATTACTCA 
CAGGAACGAG TAAACTGTTT CTGCCTAGTG TTTCCATTCA GGACATTTTA TCTCTGTGCA 
AAGACGGGAG TTGAAGCCGA TGAATGGATC AAAATACTGA GATGGAAGTT GTCAAAAATA 
AGAAAACAGC TGGATCAAGG AGAAGATACC GTCCGATCTA GGTCATTTAT CTTCAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001297682.1
CDS	131..850
Translation	MGRAELLGGNMSTQDPSELWGRADGGTDLLQDLGAKDSVKHFHVEYTGYSFKFGFNEYSSLKDFVKHFANQPLIGSETGTLMVLKHPYPREVEEPCIYESVRVHTAMQTGRTENDLVPTAPSLGTKEGYLTKQGGLVKTWKTRWFTLQRNELKYFKDQMSPEPIRILDLTECSAVQFDYSQERVNCFCLVFPFRTFYLCAKTGVEADEWIKILRWKLSKIRKQLDQGEDTVRSRSFIFK

Target ORF information:

RefSeq Version	NM_001310753.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus dual adaptor for phosphotyrosine and 3-phosphoinositides 1 (Dapp1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001310753.1

ORF Insert Sequence:

ATGGGCAGAG CAGAACTTCT AGGAGGGAAC ATGAGTACCC AGGATCCCTC AGAGCTGTGG 
GGCAGAGCAG ATGGAGGCAC AGACCTGCTC CAGGATTTGG GAGCCAAAGA CTCTGTCAAA 
CACTTTCATG TTGAATATAC TGGGTACTCG TTTAAATTCG GCTTTAATGA GTATTCATCT 
CTAAAGGATT TTGTCAAGCA TTTTGCAAAT CAGCCTTTGA TTGGAAGCGA GACAGGGACT 
CTGATGGTTT TGAAGCATCC CTATCCAAGG GAAGTGGAAG AACCTTGCAT TTACGAATCA 
GTCCGGGTTC ACACAGCGAT GCAGACAGGA AGGACAGAGA ACGACCTCGT GCCCACTGCG 
CCTTCTCTGG GCACCAAAGA AGGCTATCTC ACCAAGCAGG GAGGCCTGGT AAAGACCTGG 
AAAACAAGAT GGTTTACTTT GCAAAGGAAC GAACTGAAAT ATTTCAAAGA CCAGATGTCA 
CCAGAACCAA TTCGGATCCT AGACCTAACA GAGTGCTCAG CTGTTCAATT TGATTACTCA 
CAGGAACGAG TAAACTGTTT CTGCCTAGTG TTTCCATTCA GGACATTTTA TCTCTGTGCA 
AAGACGGGAG TTGAAGCCGA TGAATGGATC AAAATACTGA GATGGAAGTT GTCAAAAATA 
AGAAAACAGC TGGATCAAGG AGAAGATACC GTCCGATCTA GGTCATTTAT CTTCAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dapp1 ( XM_017319585.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175074.1 Mus musculus dual adaptor for phosphotyrosine and 3-phosphoinositides 1 (Dapp1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu80621
Clone ID Related Accession (Same CDS sequence)	XM_017319585.1 , XM_017319585.2
Accession Version	XM_017319585.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTTTGA AGCATCCCTA TCCAAGGGAA GTGGAAGAAC CTTGCATTTA CGAATCAGTC 
CGGGTTCACA CAGCGATGCA GACAGGAAGG ACAGAGAACG ACCTCGTGCC CACTGCGCCT 
TCTCTGGGCA CCAAAGAAGG CTATCTCACC AAGCAGGGAG GCCTGGTAAA GACCTGGAAA 
ACAAGATGGT TTACTTTGCA AAGGAACGAA CTGAAATATT TCAAAGACCA GATGTCACCA 
GAACCAATTC GGATCCTAGA CCTAACAGAG TGCTCAGCTG TTCAATTTGA TTACTCACAG 
GAACGAGTAA ACTGTTTCTG CCTAGTGTTT CCATTCAGGA CATTTTATCT CTGTGCAAAG 
ACGGGAGTTG AAGCCGATGA ATGGATCAAA ATACTGAGAT GGAAGTTGTC AAAAATAAGA 
AAACAGCTGG ATCAAGGAGA AGATACCGTC CGATCTAGGT CATTTATCTT CAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175074.1
CDS	411..887
Translation	MVLKHPYPREVEEPCIYESVRVHTAMQTGRTENDLVPTAPSLGTKEGYLTKQGGLVKTWKTRWFTLQRNELKYFKDQMSPEPIRILDLTECSAVQFDYSQERVNCFCLVFPFRTFYLCAKTGVEADEWIKILRWKLSKIRKQLDQGEDTVRSRSFIFK

Target ORF information:

RefSeq Version	XM_017319585.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus dual adaptor for phosphotyrosine and 3-phosphoinositides 1 (Dapp1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319585.1

ORF Insert Sequence:

ATGGTTTTGA AGCATCCCTA TCCAAGGGAA GTGGAAGAAC CTTGCATTTA CGAATCAGTC 
CGGGTTCACA CAGCGATGCA GACAGGAAGG ACAGAGAACG ACCTCGTGCC CACTGCGCCT 
TCTCTGGGCA CCAAAGAAGG CTATCTCACC AAGCAGGGAG GCCTGGTAAA GACCTGGAAA 
ACAAGATGGT TTACTTTGCA AAGGAACGAA CTGAAATATT TCAAAGACCA GATGTCACCA 
GAACCAATTC GGATCCTAGA CCTAACAGAG TGCTCAGCTG TTCAATTTGA TTACTCACAG 
GAACGAGTAA ACTGTTTCTG CCTAGTGTTT CCATTCAGGA CATTTTATCT CTGTGCAAAG 
ACGGGAGTTG AAGCCGATGA ATGGATCAAA ATACTGAGAT GGAAGTTGTC AAAAATAAGA 
AAACAGCTGG ATCAAGGAGA AGATACCGTC CGATCTAGGT CATTTATCTT CAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dapp1 ( XM_017319585.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175074.1 Mus musculus dual adaptor for phosphotyrosine and 3-phosphoinositides 1 (Dapp1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu80621
Clone ID Related Accession (Same CDS sequence)	XM_017319585.1 , XM_017319585.2
Accession Version	XM_017319585.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017319585.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTTTGA AGCATCCCTA TCCAAGGGAA GTGGAAGAAC CTTGCATTTA CGAATCAGTC 
CGGGTTCACA CAGCGATGCA GACAGGAAGG ACAGAGAACG ACCTCGTGCC CACTGCGCCT 
TCTCTGGGCA CCAAAGAAGG CTATCTCACC AAGCAGGGAG GCCTGGTAAA GACCTGGAAA 
ACAAGATGGT TTACTTTGCA AAGGAACGAA CTGAAATATT TCAAAGACCA GATGTCACCA 
GAACCAATTC GGATCCTAGA CCTAACAGAG TGCTCAGCTG TTCAATTTGA TTACTCACAG 
GAACGAGTAA ACTGTTTCTG CCTAGTGTTT CCATTCAGGA CATTTTATCT CTGTGCAAAG 
ACGGGAGTTG AAGCCGATGA ATGGATCAAA ATACTGAGAT GGAAGTTGTC AAAAATAAGA 
AAACAGCTGG ATCAAGGAGA AGATACCGTC CGATCTAGGT CATTTATCTT CAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175074.1
CDS	400..876
Translation	MVLKHPYPREVEEPCIYESVRVHTAMQTGRTENDLVPTAPSLGTKEGYLTKQGGLVKTWKTRWFTLQRNELKYFKDQMSPEPIRILDLTECSAVQFDYSQERVNCFCLVFPFRTFYLCAKTGVEADEWIKILRWKLSKIRKQLDQGEDTVRSRSFIFK

Target ORF information:

RefSeq Version	XM_017319585.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus dual adaptor for phosphotyrosine and 3-phosphoinositides 1 (Dapp1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319585.2

ORF Insert Sequence:

ATGGTTTTGA AGCATCCCTA TCCAAGGGAA GTGGAAGAAC CTTGCATTTA CGAATCAGTC 
CGGGTTCACA CAGCGATGCA GACAGGAAGG ACAGAGAACG ACCTCGTGCC CACTGCGCCT 
TCTCTGGGCA CCAAAGAAGG CTATCTCACC AAGCAGGGAG GCCTGGTAAA GACCTGGAAA 
ACAAGATGGT TTACTTTGCA AAGGAACGAA CTGAAATATT TCAAAGACCA GATGTCACCA 
GAACCAATTC GGATCCTAGA CCTAACAGAG TGCTCAGCTG TTCAATTTGA TTACTCACAG 
GAACGAGTAA ACTGTTTCTG CCTAGTGTTT CCATTCAGGA CATTTTATCT CTGTGCAAAG 
ACGGGAGTTG AAGCCGATGA ATGGATCAAA ATACTGAGAT GGAAGTTGTC AAAAATAAGA 
AAACAGCTGG ATCAAGGAGA AGATACCGTC CGATCTAGGT CATTTATCTT CAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The B cell adaptor molecule Bam32 is critically important for optimal antibody response and resistance to Trypanosoma congolense infection in mice.
PLoS neglected tropical diseases9(4)e0003716(2015 Apr)
Onyilagha C,Jia P,Jayachandran N,Hou S,Okwor I,Kuriakose S,Marshall A,Uzonna JE

LAT-independent Erk activation via Bam32-PLC-?1-Pak1 complexes: GTPase-independent Pak1 activation.
Molecular cell48(2)298-312(2012 Oct)
Rouquette-Jazdanian AK,Sommers CL,Kortum RL,Morrison DK,Samelson LE

The PH domain adaptor protein Bam32/DAPP1 functions in mast cells to restrain Fc?RI-induced calcium flux and granule release.
Molecular immunology48(1-3)89-97(2010 Nov-Dec)
Hou S,Pauls SD,Liu P,Marshall AJ

Bam32/DAPP1 promotes B cell adhesion and formation of polarized conjugates with T cells.
Journal of immunology (Baltimore, Md. : 1950)184(12)6961-9(2010 Jun)
Al-Alwan M,Hou S,Zhang TT,Makondo K,Marshall AJ

The pleckstrin homology domain adaptor protein Bam32/DAPP1 is required for germinal center progression.
Journal of immunology (Baltimore, Md. : 1950)184(1)164-72(2010 Jan)
Zhang TT,Al-Alwan M,Marshall AJ