Emilin2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Emilin2
Entrez Gene ID	246707
Full Name	elastin microfibril interfacer 2
Synonyms	FOAP-10
General protein information	Preferred Names elastin microfibril interfacer 2 Names EMILIN-2 basilin elastin microfibril interface located protein 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17\|17 E1.3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001357336.1	NP_001344265.1	EMILIN-2 isoform 2
NM_145158.3	NP_660140.1	EMILIN-2 precursor
XM_006524285.3	XP_006524348.1	EMILIN-2 isoform X1
XM_006524286.3	XP_006524349.1	EMILIN-2 isoform X2
NM_145158.4	NP_660140.1	EMILIN-2 isoform 1 precursor
NM_001357338.1	NP_001344267.1	EMILIN-2 isoform 4 precursor
XM_011246461.2	XP_011244763.1	EMILIN-2 isoform X3
NM_001357337.1	NP_001344266.1	EMILIN-2 isoform 3 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	EMILIN2	XP_003315909.1
Mus musculus (house mouse)	Emilin2	NP_660140.1
Gallus gallus (chicken)	EMILIN2	XP_419145.4
Danio rerio (zebrafish)	LOC100001725	XP_001341669.4
Canis lupus familiaris (dog)	EMILIN2	XP_005623089.1
Xenopus tropicalis (tropical clawed frog)	emilin2	XP_002938956.2
	EMILIN2	XP_001086149.1
Bos taurus (cattle)	EMILIN2	NP_001137341.1
Homo sapiens (human)	EMILIN2	NP_114437.2
Rattus norvegicus (Norway rat)	Emilin2	XP_006245732.1

Gene Ontology
Bibliography

Related articles in PubMed

Altered feto-placental vascularization, feto-placental malperfusion and fetal growth restriction in mice with
Lacko LA, Hurtado R, Hinds S, Poulos MG, Butler JM, Stuhlmann H
Development (Cambridge, England)144(13)2469-2479(2017 07)

EMILIN2 regulates platelet activation, thrombus formation, and clot retraction.
Huang M, Sannaningaiah D, Zhao N, Gong Y, Grondolsky J, Hoover-Plow J
PloS one10(2)e0115284(2015)

Multiple-interactions among EMILIN1 and EMILIN2 N- and C-terminal domains.
Bot S, Andreuzzi E, Capuano A, Schiavinato A, Colombatti A, Doliana R
Matrix biology : journal of the International Society for Matrix Biology4144-55(2015 Jan)

Extracellular matrix secretion by cardiac fibroblasts: role of microRNA-29b and microRNA-30c.
Abonnenc M, Nabeebaccus AA, Mayr U, Barallobre-Barreiro J, Dong X, Cuello F, Sur S, Drozdov I, Langley SR, Lu R, Stathopoulou K, Didangelos A, Yin X, Zimmermann WH, Shah AM, Zampetaki A, Mayr M
Circulation research113(10)1138-47(2013 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

EMILIN1 may attenuate the effects of EMILIN2 on platelet aggregation and thrombosis.
Title: EMILIN2 regulates platelet activation, thrombus formation, and clot retraction.

Data suggested mechanisms for homo- and hetero-typic EMILINs multimers formation: EMILIN1 or EMILIN2 alone can form trimers and multimers in the absence of each other or they can co-polymerize in a head-to-tail fashion to form hetero-typic multimers.
Title: Multiple-interactions among EMILIN1 and EMILIN2 N- and C-terminal domains.

cochlear emilin-2 mRNA is expressed in the tympanic border cells and an antibody detected protein in the extracellular matrix surrounding the collagenous fibers in the cochlear basilar membrane
Title: An emilin family extracellular matrix protein identified in the cochlear basilar membrane.

The following Emilin2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Emilin2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu74270	NM_001357336.1 Latest version!	Mus musculus elastin microfibril interfacer 2 (Emilin2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00
OMu74270	XM_011246461.2 Latest version!	Mus musculus elastin microfibril interfacer 2 (Emilin2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00
OMu11369	NM_145158.4 Latest version!	Mus musculus elastin microfibril interfacer 2 (Emilin2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
OMu53594	NM_001357337.1 Latest version!	Mus musculus elastin microfibril interfacer 2 (Emilin2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu53595	NM_001357338.1 Latest version!	Mus musculus elastin microfibril interfacer 2 (Emilin2), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu53594	XM_006524285.3 Latest version!	Mus musculus elastin microfibril interfacer 2 (Emilin2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu53595	XM_006524286.3 Latest version!	Mus musculus elastin microfibril interfacer 2 (Emilin2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OMu11369	NM_145158.3	Mus musculus elastin microfibril interfacer 2 (Emilin2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Emilin2 ( NM_001357336.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001344265.1 Mus musculus elastin microfibril interfacer 2 (Emilin2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu74270
Clone ID Related Accession (Same CDS sequence)	NM_001357336.1 , XM_011246461.2
Accession Version	NM_001357336.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2982bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	EMILIN-2 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC126942.4 and BY582438.1. On Oct 26, 2017 this sequence version replaced XM_011246461.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK171303.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164135, SAMN01164137 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCTGTC CATCCGCCCT GGTGTATCGG GTAAACTTTA GACCCAGATT TGTCACTAGA 
TACAAGATAG TGACACAGTT GGAATGGAGA TGCTGTCCTG GCTTTAGAGG ACCAGACTGC 
CAAGAAGGTC CCAAAGACCA CATGAAGACC CCCCGGCCCC CATCAGCTCG ACCAAAAAAC 
AACCTGAAGA AAGCCACAGA TACAGATCCA AGCCAAGTTT CTCAGCCTAA GAAAACGTTG 
TCCCCAACAA ATGCAGTGGA ACCAGGTCAG GTTGCAGATG CAAAGCAAGG CCCTCCGGAA 
CTTCAGCAAT CCAAAGTCCA GGTGCTGGAG GAGAAGGTCG TCCGACTCAC CAGGATGGTG 
CTGGATCTCC AGTCCACCGT GGTAGGCCTG AAGGAGAACC TCAAACACAC CATTCAAGAT 
GATGGCAGAA AAGAGCCAGA CTCCTGGCTC GGTCCCCTGC ACCCACAGCC AACGCCTGAC 
AGCCCCCTTG CGGGGGACGC AGAGCCGAGC CAGCTTCCCG GAATCCCCAG CAGCAAGGAA 
TCTGGTATGA AGGACATCAA GTCTGAGTTG GCTGAGGTCA AAGACACACT GAAGACCAAG 
AGTGACAAGC TAGAAGAGCT GGACGGGAAG GTGAAGGGCT ATGAAGGACA GCTCAAGCAG 
CTGCAGGAGG CGGCTCAGGG TCCCACGGTC ACCATGACAA CCAATGAGCT GTACCAAGCC 
TATGTGGATA GCAAGATCGA TGCCCTGAGG GAGGAGCTCA TGGAAGGCAT GGACCGGAAG 
CTGGCTGACC TGAAGAATAC GTGCGAGTAC AAGCTGGTGG GGCTCCAGCA GCAGTGTGAT 
GACTACGGCA GCAGCTACCT CGGAGTGATA GAGCTGATCG GGGAGAAGGA GGCGAGCCTG 
AAAAAAGACA TTGCTGACCT GCGAGCCCAG CTGCAGGATC CTGTAGCCCA ACCAAGCTGT 
TGCAATGGTC AAAAGAGCAG CGATTTCGGC CCACAGATCA AGGCGCTAGA CCAGAAAATC 
GAGAGAGTTG CCGAGGCCAC CAGGATGCTG AATGGACGGC TGGACAATGA GTTCGACCGC 
CTCTCCGTTC CAGAGCCAGA CGCAGACTTT GATGCGAGAT GGACCGAGCT CGACGCAAGG 
ATCAATGTGA CGGAGAAGAA CGCAGAGGAG CATTGCTTTT ACATTGAGGA GACCCTGCGG 
GGGACCATCA ACGGCGAAGT GGATGACTTG AGGAAGCTTC TGAATGAGAA GATCCACTCC 
CTGGAAGACC GTCTGGGAAT TGTTCTGCAA GCGGCTAACA GCTCAGACGT GGAGTTGACA 
CCCATGGGCC CAGCCTTGCC AGAGCAGCCA GGGGCAGAGA ATGAGCAGGT CCTAATGGAG 
TTGAGCCGCC TGAAGGACAA AGTTCAAGTG GTTGAGGACT TCTGCCTGCA GAGCTTGCCC 
CACGGGATAG ATGGAGCTTT GCCTAGTGTA GAGGACCTCA CGCATGTCAG CCTGAGCCTT 
TTAGAATCCC TCAATGATAC GATGCACAGG CAGTTCCAAG AAACGAGTCA CAGCATCCAG 
AAACTCCAAG AGGACGTCAA TGCCCTCCAT TCTCAACTGA ATCACTCCGA ATGTACGGGG 
ACTTACTTGC AGAACGGAGT GAGCGACAGC CGAACAGGTG ACAGCATGGA AGCAAGTGGG 
TTTACTAAGA CAGGGGAGCA AGAGAGGACA GTGGGGACTG TCCCGTCTCC TGGGACCCCC 
GCGGCTCCAT GCTGTGGTCA GCTGGAGGAG CGCTGGCAGA AGCTGCAGAA CCAAATGTTG 
GCTGAGCTAG ACACGTGTAA GGAGAGTGCT CACGGGGTTC AGAGTGGGGT GTCTGCGATC 
GAGGGCAGGG TGTTCCAGCT GGAGCAGACA TGCAGAAGGC TGGATACCAT CTCAGGGAGT 
CTGCAGAGGA TCAAGGAGGG GCTCGGTAAG CATGTGGGCA GCTTGTGGAA CTGCATCCGG 
CAGATGAACG GGACGCTCAA ATCGCACTCC AGAGACATCT CTGGCCTCAA GAATTCTGTG 
CAGCAATTCT ATAGCCATGT TTTCCAGATT TCTACCGACT TGCAAGATCT GGTCAAGTTT 
CAGCCATCAG CAACAGAGGA ACCCTCAGAA GCAACCGAGG GACCGTCTGG GAAGACTCCC 
CTAGAATCCA CCAGACCTTC AGAGGAGGCC CCCACTGAAC CACCCCGGCT GACCCCACTG 
CCAGAAGACC CTGCAGGACC ACCACAGACA GGCCAGCAGC CAGTATTGCC CCAGAGGCCC 
CTGCAGCCAC CACCTTTGCC TGCCTGGCCT GGTCGCACGG GGTTGCCCTT CTTGCCTGGC 
TCTAGTGGCG TCATCATGGA GACTGGAGAG GCGGGGCCTC CAGGCAGGAT GGGCGTGTCC 
GGAAGGGGCC TGCCTCGGGG AGTGGATGGC CAGATGGGGC AGGGGCCCAT TCATAGCTCG 
GAAGGCTATG CAGGGGCACC AGGCTACCCC AAGTCACCTC CTGTAACCAC CCCAGGGGTG 
CCGCTGCCCA CTCTGGTGTC CTTTTCAGCC GGTCTTACCC AGAAGCCTTT TCCCAGCGAT 
GGAGGCGTTG TTCTCTTCAA TAAGGTGCTG GTGAACGATG GGGATGTATA CAACCCCAAC 
ACTGGGATCT TCACCGCACC ATACGACGGG CGCTACTTGA TCACAGCCAC CCTCACCCCT 
GAGAGAGACA CCTATGTGGA AGCCGTCCTG TCTGTCTCCA ACGCCAGTGT TGCCCAGCTG 
CACACGGCTG GGTACAGGAG AGAGTTCCTG GAATACCATC GTCCCCCTGG GGCTGTGCAT 
ACCTGTGGGG GCCCGGGGGC CTTCCACCTC ATTGTGCACT TGAAGGCAGG AGATGGAGTC 
AATGTTGTGG TGACTGGGGG CAGACTGGCT CACACAGACT TTGATGAAAT GTACTCCACC 
TTCAGTGGTG TTTTCTTGTA CCCTTTCCTT TCTCACCTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001344265.1
CDS	320..3301
Translation	MPCPSALVYRVNFRPRFVTRYKIVTQLEWRCCPGFRGPDCQEGPKDHMKTPRPPSARPKNNLKKATDTDPSQVSQPKKTLSPTNAVEPGQVADAKQGPPELQQSKVQVLEEKVVRLTRMVLDLQSTVVGLKENLKHTIQDDGRKEPDSWLGPLHPQPTPDSPLAGDAEPSQLPGIPSSKESGMKDIKSELAEVKDTLKTKSDKLEELDGKVKGYEGQLKQLQEAAQGPTVTMTTNELYQAYVDSKIDALREELMEGMDRKLADLKNTCEYKLVGLQQQCDDYGSSYLGVIELIGEKEASLKKDIADLRAQLQDPVAQPSCCNGQKSSDFGPQIKALDQKIERVAEATRMLNGRLDNEFDRLSVPEPDADFDARWTELDARINVTEKNAEEHCFYIEETLRGTINGEVDDLRKLLNEKIHSLEDRLGIVLQAANSSDVELTPMGPALPEQPGAENEQVLMELSRLKDKVQVVEDFCLQSLPHGIDGALPSVEDLTHVSLSLLESLNDTMHRQFQETSHSIQKLQEDVNALHSQLNHSECTGTYLQNGVSDSRTGDSMEASGFTKTGEQERTVGTVPSPGTPAAPCCGQLEERWQKLQNQMLAELDTCKESAHGVQSGVSAIEGRVFQLEQTCRRLDTISGSLQRIKEGLGKHVGSLWNCIRQMNGTLKSHSRDISGLKNSVQQFYSHVFQISTDLQDLVKFQPSATEEPSEATEGPSGKTPLESTRPSEEAPTEPPRLTPLPEDPAGPPQTGQQPVLPQRPLQPPPLPAWPGRTGLPFLPGSSGVIMETGEAGPPGRMGVSGRGLPRGVDGQMGQGPIHSSEGYAGAPGYPKSPPVTTPGVPLPTLVSFSAGLTQKPFPSDGGVVLFNKVLVNDGDVYNPNTGIFTAPYDGRYLITATLTPERDTYVEAVLSVSNASVAQLHTAGYRREFLEYHRPPGAVHTCGGPGAFHLIVHLKAGDGVNVVVTGGRLAHTDFDEMYSTFSGVFLYPFLSHL

Target ORF information:

RefSeq Version	NM_001357336.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus elastin microfibril interfacer 2 (Emilin2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001357336.1

ORF Insert Sequence:

ATGCCCTGTC CATCCGCCCT GGTGTATCGG GTAAACTTTA GACCCAGATT TGTCACTAGA 
TACAAGATAG TGACACAGTT GGAATGGAGA TGCTGTCCTG GCTTTAGAGG ACCAGACTGC 
CAAGAAGGTC CCAAAGACCA CATGAAGACC CCCCGGCCCC CATCAGCTCG ACCAAAAAAC 
AACCTGAAGA AAGCCACAGA TACAGATCCA AGCCAAGTTT CTCAGCCTAA GAAAACGTTG 
TCCCCAACAA ATGCAGTGGA ACCAGGTCAG GTTGCAGATG CAAAGCAAGG CCCTCCGGAA 
CTTCAGCAAT CCAAAGTCCA GGTGCTGGAG GAGAAGGTCG TCCGACTCAC CAGGATGGTG 
CTGGATCTCC AGTCCACCGT GGTAGGCCTG AAGGAGAACC TCAAACACAC CATTCAAGAT 
GATGGCAGAA AAGAGCCAGA CTCCTGGCTC GGTCCCCTGC ACCCACAGCC AACGCCTGAC 
AGCCCCCTTG CGGGGGACGC AGAGCCGAGC CAGCTTCCCG GAATCCCCAG CAGCAAGGAA 
TCTGGTATGA AGGACATCAA GTCTGAGTTG GCTGAGGTCA AAGACACACT GAAGACCAAG 
AGTGACAAGC TAGAAGAGCT GGACGGGAAG GTGAAGGGCT ATGAAGGACA GCTCAAGCAG 
CTGCAGGAGG CGGCTCAGGG TCCCACGGTC ACCATGACAA CCAATGAGCT GTACCAAGCC 
TATGTGGATA GCAAGATCGA TGCCCTGAGG GAGGAGCTCA TGGAAGGCAT GGACCGGAAG 
CTGGCTGACC TGAAGAATAC GTGCGAGTAC AAGCTGGTGG GGCTCCAGCA GCAGTGTGAT 
GACTACGGCA GCAGCTACCT CGGAGTGATA GAGCTGATCG GGGAGAAGGA GGCGAGCCTG 
AAAAAAGACA TTGCTGACCT GCGAGCCCAG CTGCAGGATC CTGTAGCCCA ACCAAGCTGT 
TGCAATGGTC AAAAGAGCAG CGATTTCGGC CCACAGATCA AGGCGCTAGA CCAGAAAATC 
GAGAGAGTTG CCGAGGCCAC CAGGATGCTG AATGGACGGC TGGACAATGA GTTCGACCGC 
CTCTCCGTTC CAGAGCCAGA CGCAGACTTT GATGCGAGAT GGACCGAGCT CGACGCAAGG 
ATCAATGTGA CGGAGAAGAA CGCAGAGGAG CATTGCTTTT ACATTGAGGA GACCCTGCGG 
GGGACCATCA ACGGCGAAGT GGATGACTTG AGGAAGCTTC TGAATGAGAA GATCCACTCC 
CTGGAAGACC GTCTGGGAAT TGTTCTGCAA GCGGCTAACA GCTCAGACGT GGAGTTGACA 
CCCATGGGCC CAGCCTTGCC AGAGCAGCCA GGGGCAGAGA ATGAGCAGGT CCTAATGGAG 
TTGAGCCGCC TGAAGGACAA AGTTCAAGTG GTTGAGGACT TCTGCCTGCA GAGCTTGCCC 
CACGGGATAG ATGGAGCTTT GCCTAGTGTA GAGGACCTCA CGCATGTCAG CCTGAGCCTT 
TTAGAATCCC TCAATGATAC GATGCACAGG CAGTTCCAAG AAACGAGTCA CAGCATCCAG 
AAACTCCAAG AGGACGTCAA TGCCCTCCAT TCTCAACTGA ATCACTCCGA ATGTACGGGG 
ACTTACTTGC AGAACGGAGT GAGCGACAGC CGAACAGGTG ACAGCATGGA AGCAAGTGGG 
TTTACTAAGA CAGGGGAGCA AGAGAGGACA GTGGGGACTG TCCCGTCTCC TGGGACCCCC 
GCGGCTCCAT GCTGTGGTCA GCTGGAGGAG CGCTGGCAGA AGCTGCAGAA CCAAATGTTG 
GCTGAGCTAG ACACGTGTAA GGAGAGTGCT CACGGGGTTC AGAGTGGGGT GTCTGCGATC 
GAGGGCAGGG TGTTCCAGCT GGAGCAGACA TGCAGAAGGC TGGATACCAT CTCAGGGAGT 
CTGCAGAGGA TCAAGGAGGG GCTCGGTAAG CATGTGGGCA GCTTGTGGAA CTGCATCCGG 
CAGATGAACG GGACGCTCAA ATCGCACTCC AGAGACATCT CTGGCCTCAA GAATTCTGTG 
CAGCAATTCT ATAGCCATGT TTTCCAGATT TCTACCGACT TGCAAGATCT GGTCAAGTTT 
CAGCCATCAG CAACAGAGGA ACCCTCAGAA GCAACCGAGG GACCGTCTGG GAAGACTCCC 
CTAGAATCCA CCAGACCTTC AGAGGAGGCC CCCACTGAAC CACCCCGGCT GACCCCACTG 
CCAGAAGACC CTGCAGGACC ACCACAGACA GGCCAGCAGC CAGTATTGCC CCAGAGGCCC 
CTGCAGCCAC CACCTTTGCC TGCCTGGCCT GGTCGCACGG GGTTGCCCTT CTTGCCTGGC 
TCTAGTGGCG TCATCATGGA GACTGGAGAG GCGGGGCCTC CAGGCAGGAT GGGCGTGTCC 
GGAAGGGGCC TGCCTCGGGG AGTGGATGGC CAGATGGGGC AGGGGCCCAT TCATAGCTCG 
GAAGGCTATG CAGGGGCACC AGGCTACCCC AAGTCACCTC CTGTAACCAC CCCAGGGGTG 
CCGCTGCCCA CTCTGGTGTC CTTTTCAGCC GGTCTTACCC AGAAGCCTTT TCCCAGCGAT 
GGAGGCGTTG TTCTCTTCAA TAAGGTGCTG GTGAACGATG GGGATGTATA CAACCCCAAC 
ACTGGGATCT TCACCGCACC ATACGACGGG CGCTACTTGA TCACAGCCAC CCTCACCCCT 
GAGAGAGACA CCTATGTGGA AGCCGTCCTG TCTGTCTCCA ACGCCAGTGT TGCCCAGCTG 
CACACGGCTG GGTACAGGAG AGAGTTCCTG GAATACCATC GTCCCCCTGG GGCTGTGCAT 
ACCTGTGGGG GCCCGGGGGC CTTCCACCTC ATTGTGCACT TGAAGGCAGG AGATGGAGTC 
AATGTTGTGG TGACTGGGGG CAGACTGGCT CACACAGACT TTGATGAAAT GTACTCCACC 
TTCAGTGGTG TTTTCTTGTA CCCTTTCCTT TCTCACCTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Emilin2 ( XM_011246461.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244763.1 Mus musculus elastin microfibril interfacer 2 (Emilin2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu74270
Clone ID Related Accession (Same CDS sequence)	NM_001357336.1 , XM_011246461.2
Accession Version	XM_011246461.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2982bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	EMILIN-2 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011246461.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTGTC CATCCGCCCT GGTGTATCGG GTAAACTTTA GACCCAGATT TGTCACTAGA 
TACAAGATAG TGACACAGTT GGAATGGAGA TGCTGTCCTG GCTTTAGAGG ACCAGACTGC 
CAAGAAGGTC CCAAAGACCA CATGAAGACC CCCCGGCCCC CATCAGCTCG ACCAAAAAAC 
AACCTGAAGA AAGCCACAGA TACAGATCCA AGCCAAGTTT CTCAGCCTAA GAAAACGTTG 
TCCCCAACAA ATGCAGTGGA ACCAGGTCAG GTTGCAGATG CAAAGCAAGG CCCTCCGGAA 
CTTCAGCAAT CCAAAGTCCA GGTGCTGGAG GAGAAGGTCG TCCGACTCAC CAGGATGGTG 
CTGGATCTCC AGTCCACCGT GGTAGGCCTG AAGGAGAACC TCAAACACAC CATTCAAGAT 
GATGGCAGAA AAGAGCCAGA CTCCTGGCTC GGTCCCCTGC ACCCACAGCC AACGCCTGAC 
AGCCCCCTTG CGGGGGACGC AGAGCCGAGC CAGCTTCCCG GAATCCCCAG CAGCAAGGAA 
TCTGGTATGA AGGACATCAA GTCTGAGTTG GCTGAGGTCA AAGACACACT GAAGACCAAG 
AGTGACAAGC TAGAAGAGCT GGACGGGAAG GTGAAGGGCT ATGAAGGACA GCTCAAGCAG 
CTGCAGGAGG CGGCTCAGGG TCCCACGGTC ACCATGACAA CCAATGAGCT GTACCAAGCC 
TATGTGGATA GCAAGATCGA TGCCCTGAGG GAGGAGCTCA TGGAAGGCAT GGACCGGAAG 
CTGGCTGACC TGAAGAATAC GTGCGAGTAC AAGCTGGTGG GGCTCCAGCA GCAGTGTGAT 
GACTACGGCA GCAGCTACCT CGGAGTGATA GAGCTGATCG GGGAGAAGGA GGCGAGCCTG 
AAAAAAGACA TTGCTGACCT GCGAGCCCAG CTGCAGGATC CTGTAGCCCA ACCAAGCTGT 
TGCAATGGTC AAAAGAGCAG CGATTTCGGC CCACAGATCA AGGCGCTAGA CCAGAAAATC 
GAGAGAGTTG CCGAGGCCAC CAGGATGCTG AATGGACGGC TGGACAATGA GTTCGACCGC 
CTCTCCGTTC CAGAGCCAGA CGCAGACTTT GATGCGAGAT GGACCGAGCT CGACGCAAGG 
ATCAATGTGA CGGAGAAGAA CGCAGAGGAG CATTGCTTTT ACATTGAGGA GACCCTGCGG 
GGGACCATCA ACGGCGAAGT GGATGACTTG AGGAAGCTTC TGAATGAGAA GATCCACTCC 
CTGGAAGACC GTCTGGGAAT TGTTCTGCAA GCGGCTAACA GCTCAGACGT GGAGTTGACA 
CCCATGGGCC CAGCCTTGCC AGAGCAGCCA GGGGCAGAGA ATGAGCAGGT CCTAATGGAG 
TTGAGCCGCC TGAAGGACAA AGTTCAAGTG GTTGAGGACT TCTGCCTGCA GAGCTTGCCC 
CACGGGATAG ATGGAGCTTT GCCTAGTGTA GAGGACCTCA CGCATGTCAG CCTGAGCCTT 
TTAGAATCCC TCAATGATAC GATGCACAGG CAGTTCCAAG AAACGAGTCA CAGCATCCAG 
AAACTCCAAG AGGACGTCAA TGCCCTCCAT TCTCAACTGA ATCACTCCGA ATGTACGGGG 
ACTTACTTGC AGAACGGAGT GAGCGACAGC CGAACAGGTG ACAGCATGGA AGCAAGTGGG 
TTTACTAAGA CAGGGGAGCA AGAGAGGACA GTGGGGACTG TCCCGTCTCC TGGGACCCCC 
GCGGCTCCAT GCTGTGGTCA GCTGGAGGAG CGCTGGCAGA AGCTGCAGAA CCAAATGTTG 
GCTGAGCTAG ACACGTGTAA GGAGAGTGCT CACGGGGTTC AGAGTGGGGT GTCTGCGATC 
GAGGGCAGGG TGTTCCAGCT GGAGCAGACA TGCAGAAGGC TGGATACCAT CTCAGGGAGT 
CTGCAGAGGA TCAAGGAGGG GCTCGGTAAG CATGTGGGCA GCTTGTGGAA CTGCATCCGG 
CAGATGAACG GGACGCTCAA ATCGCACTCC AGAGACATCT CTGGCCTCAA GAATTCTGTG 
CAGCAATTCT ATAGCCATGT TTTCCAGATT TCTACCGACT TGCAAGATCT GGTCAAGTTT 
CAGCCATCAG CAACAGAGGA ACCCTCAGAA GCAACCGAGG GACCGTCTGG GAAGACTCCC 
CTAGAATCCA CCAGACCTTC AGAGGAGGCC CCCACTGAAC CACCCCGGCT GACCCCACTG 
CCAGAAGACC CTGCAGGACC ACCACAGACA GGCCAGCAGC CAGTATTGCC CCAGAGGCCC 
CTGCAGCCAC CACCTTTGCC TGCCTGGCCT GGTCGCACGG GGTTGCCCTT CTTGCCTGGC 
TCTAGTGGCG TCATCATGGA GACTGGAGAG GCGGGGCCTC CAGGCAGGAT GGGCGTGTCC 
GGAAGGGGCC TGCCTCGGGG AGTGGATGGC CAGATGGGGC AGGGGCCCAT TCATAGCTCG 
GAAGGCTATG CAGGGGCACC AGGCTACCCC AAGTCACCTC CTGTAACCAC CCCAGGGGTG 
CCGCTGCCCA CTCTGGTGTC CTTTTCAGCC GGTCTTACCC AGAAGCCTTT TCCCAGCGAT 
GGAGGCGTTG TTCTCTTCAA TAAGGTGCTG GTGAACGATG GGGATGTATA CAACCCCAAC 
ACTGGGATCT TCACCGCACC ATACGACGGG CGCTACTTGA TCACAGCCAC CCTCACCCCT 
GAGAGAGACA CCTATGTGGA AGCCGTCCTG TCTGTCTCCA ACGCCAGTGT TGCCCAGCTG 
CACACGGCTG GGTACAGGAG AGAGTTCCTG GAATACCATC GTCCCCCTGG GGCTGTGCAT 
ACCTGTGGGG GCCCGGGGGC CTTCCACCTC ATTGTGCACT TGAAGGCAGG AGATGGAGTC 
AATGTTGTGG TGACTGGGGG CAGACTGGCT CACACAGACT TTGATGAAAT GTACTCCACC 
TTCAGTGGTG TTTTCTTGTA CCCTTTCCTT TCTCACCTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244763.1
CDS	397..3378
Translation	MPCPSALVYRVNFRPRFVTRYKIVTQLEWRCCPGFRGPDCQEGPKDHMKTPRPPSARPKNNLKKATDTDPSQVSQPKKTLSPTNAVEPGQVADAKQGPPELQQSKVQVLEEKVVRLTRMVLDLQSTVVGLKENLKHTIQDDGRKEPDSWLGPLHPQPTPDSPLAGDAEPSQLPGIPSSKESGMKDIKSELAEVKDTLKTKSDKLEELDGKVKGYEGQLKQLQEAAQGPTVTMTTNELYQAYVDSKIDALREELMEGMDRKLADLKNTCEYKLVGLQQQCDDYGSSYLGVIELIGEKEASLKKDIADLRAQLQDPVAQPSCCNGQKSSDFGPQIKALDQKIERVAEATRMLNGRLDNEFDRLSVPEPDADFDARWTELDARINVTEKNAEEHCFYIEETLRGTINGEVDDLRKLLNEKIHSLEDRLGIVLQAANSSDVELTPMGPALPEQPGAENEQVLMELSRLKDKVQVVEDFCLQSLPHGIDGALPSVEDLTHVSLSLLESLNDTMHRQFQETSHSIQKLQEDVNALHSQLNHSECTGTYLQNGVSDSRTGDSMEASGFTKTGEQERTVGTVPSPGTPAAPCCGQLEERWQKLQNQMLAELDTCKESAHGVQSGVSAIEGRVFQLEQTCRRLDTISGSLQRIKEGLGKHVGSLWNCIRQMNGTLKSHSRDISGLKNSVQQFYSHVFQISTDLQDLVKFQPSATEEPSEATEGPSGKTPLESTRPSEEAPTEPPRLTPLPEDPAGPPQTGQQPVLPQRPLQPPPLPAWPGRTGLPFLPGSSGVIMETGEAGPPGRMGVSGRGLPRGVDGQMGQGPIHSSEGYAGAPGYPKSPPVTTPGVPLPTLVSFSAGLTQKPFPSDGGVVLFNKVLVNDGDVYNPNTGIFTAPYDGRYLITATLTPERDTYVEAVLSVSNASVAQLHTAGYRREFLEYHRPPGAVHTCGGPGAFHLIVHLKAGDGVNVVVTGGRLAHTDFDEMYSTFSGVFLYPFLSHL

Target ORF information:

RefSeq Version	XM_011246461.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus elastin microfibril interfacer 2 (Emilin2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011246461.2

ORF Insert Sequence:

ATGCCCTGTC CATCCGCCCT GGTGTATCGG GTAAACTTTA GACCCAGATT TGTCACTAGA 
TACAAGATAG TGACACAGTT GGAATGGAGA TGCTGTCCTG GCTTTAGAGG ACCAGACTGC 
CAAGAAGGTC CCAAAGACCA CATGAAGACC CCCCGGCCCC CATCAGCTCG ACCAAAAAAC 
AACCTGAAGA AAGCCACAGA TACAGATCCA AGCCAAGTTT CTCAGCCTAA GAAAACGTTG 
TCCCCAACAA ATGCAGTGGA ACCAGGTCAG GTTGCAGATG CAAAGCAAGG CCCTCCGGAA 
CTTCAGCAAT CCAAAGTCCA GGTGCTGGAG GAGAAGGTCG TCCGACTCAC CAGGATGGTG 
CTGGATCTCC AGTCCACCGT GGTAGGCCTG AAGGAGAACC TCAAACACAC CATTCAAGAT 
GATGGCAGAA AAGAGCCAGA CTCCTGGCTC GGTCCCCTGC ACCCACAGCC AACGCCTGAC 
AGCCCCCTTG CGGGGGACGC AGAGCCGAGC CAGCTTCCCG GAATCCCCAG CAGCAAGGAA 
TCTGGTATGA AGGACATCAA GTCTGAGTTG GCTGAGGTCA AAGACACACT GAAGACCAAG 
AGTGACAAGC TAGAAGAGCT GGACGGGAAG GTGAAGGGCT ATGAAGGACA GCTCAAGCAG 
CTGCAGGAGG CGGCTCAGGG TCCCACGGTC ACCATGACAA CCAATGAGCT GTACCAAGCC 
TATGTGGATA GCAAGATCGA TGCCCTGAGG GAGGAGCTCA TGGAAGGCAT GGACCGGAAG 
CTGGCTGACC TGAAGAATAC GTGCGAGTAC AAGCTGGTGG GGCTCCAGCA GCAGTGTGAT 
GACTACGGCA GCAGCTACCT CGGAGTGATA GAGCTGATCG GGGAGAAGGA GGCGAGCCTG 
AAAAAAGACA TTGCTGACCT GCGAGCCCAG CTGCAGGATC CTGTAGCCCA ACCAAGCTGT 
TGCAATGGTC AAAAGAGCAG CGATTTCGGC CCACAGATCA AGGCGCTAGA CCAGAAAATC 
GAGAGAGTTG CCGAGGCCAC CAGGATGCTG AATGGACGGC TGGACAATGA GTTCGACCGC 
CTCTCCGTTC CAGAGCCAGA CGCAGACTTT GATGCGAGAT GGACCGAGCT CGACGCAAGG 
ATCAATGTGA CGGAGAAGAA CGCAGAGGAG CATTGCTTTT ACATTGAGGA GACCCTGCGG 
GGGACCATCA ACGGCGAAGT GGATGACTTG AGGAAGCTTC TGAATGAGAA GATCCACTCC 
CTGGAAGACC GTCTGGGAAT TGTTCTGCAA GCGGCTAACA GCTCAGACGT GGAGTTGACA 
CCCATGGGCC CAGCCTTGCC AGAGCAGCCA GGGGCAGAGA ATGAGCAGGT CCTAATGGAG 
TTGAGCCGCC TGAAGGACAA AGTTCAAGTG GTTGAGGACT TCTGCCTGCA GAGCTTGCCC 
CACGGGATAG ATGGAGCTTT GCCTAGTGTA GAGGACCTCA CGCATGTCAG CCTGAGCCTT 
TTAGAATCCC TCAATGATAC GATGCACAGG CAGTTCCAAG AAACGAGTCA CAGCATCCAG 
AAACTCCAAG AGGACGTCAA TGCCCTCCAT TCTCAACTGA ATCACTCCGA ATGTACGGGG 
ACTTACTTGC AGAACGGAGT GAGCGACAGC CGAACAGGTG ACAGCATGGA AGCAAGTGGG 
TTTACTAAGA CAGGGGAGCA AGAGAGGACA GTGGGGACTG TCCCGTCTCC TGGGACCCCC 
GCGGCTCCAT GCTGTGGTCA GCTGGAGGAG CGCTGGCAGA AGCTGCAGAA CCAAATGTTG 
GCTGAGCTAG ACACGTGTAA GGAGAGTGCT CACGGGGTTC AGAGTGGGGT GTCTGCGATC 
GAGGGCAGGG TGTTCCAGCT GGAGCAGACA TGCAGAAGGC TGGATACCAT CTCAGGGAGT 
CTGCAGAGGA TCAAGGAGGG GCTCGGTAAG CATGTGGGCA GCTTGTGGAA CTGCATCCGG 
CAGATGAACG GGACGCTCAA ATCGCACTCC AGAGACATCT CTGGCCTCAA GAATTCTGTG 
CAGCAATTCT ATAGCCATGT TTTCCAGATT TCTACCGACT TGCAAGATCT GGTCAAGTTT 
CAGCCATCAG CAACAGAGGA ACCCTCAGAA GCAACCGAGG GACCGTCTGG GAAGACTCCC 
CTAGAATCCA CCAGACCTTC AGAGGAGGCC CCCACTGAAC CACCCCGGCT GACCCCACTG 
CCAGAAGACC CTGCAGGACC ACCACAGACA GGCCAGCAGC CAGTATTGCC CCAGAGGCCC 
CTGCAGCCAC CACCTTTGCC TGCCTGGCCT GGTCGCACGG GGTTGCCCTT CTTGCCTGGC 
TCTAGTGGCG TCATCATGGA GACTGGAGAG GCGGGGCCTC CAGGCAGGAT GGGCGTGTCC 
GGAAGGGGCC TGCCTCGGGG AGTGGATGGC CAGATGGGGC AGGGGCCCAT TCATAGCTCG 
GAAGGCTATG CAGGGGCACC AGGCTACCCC AAGTCACCTC CTGTAACCAC CCCAGGGGTG 
CCGCTGCCCA CTCTGGTGTC CTTTTCAGCC GGTCTTACCC AGAAGCCTTT TCCCAGCGAT 
GGAGGCGTTG TTCTCTTCAA TAAGGTGCTG GTGAACGATG GGGATGTATA CAACCCCAAC 
ACTGGGATCT TCACCGCACC ATACGACGGG CGCTACTTGA TCACAGCCAC CCTCACCCCT 
GAGAGAGACA CCTATGTGGA AGCCGTCCTG TCTGTCTCCA ACGCCAGTGT TGCCCAGCTG 
CACACGGCTG GGTACAGGAG AGAGTTCCTG GAATACCATC GTCCCCCTGG GGCTGTGCAT 
ACCTGTGGGG GCCCGGGGGC CTTCCACCTC ATTGTGCACT TGAAGGCAGG AGATGGAGTC 
AATGTTGTGG TGACTGGGGG CAGACTGGCT CACACAGACT TTGATGAAAT GTACTCCACC 
TTCAGTGGTG TTTTCTTGTA CCCTTTCCTT TCTCACCTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Emilin2 ( NM_145158.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_660140.1 Mus musculus elastin microfibril interfacer 2 (Emilin2), mRNA.

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CloneID	OMu11369
Clone ID Related Accession (Same CDS sequence)	NM_145158.3 , NM_145158.4
Accession Version	NM_145158.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-29
Organism	Mus musculus(House mouse)
Product	EMILIN-2 precursor
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AK155909.1. On Jun 10, 2007 this sequence version replaced NM_145158.2. ##Evidence-Data-START## Transcript exon combination :: AK155909.1, AK170822.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164135 [ECO:0000348] ##Evidence-Data-END##

ATGTGTCAGG AGACCCCGCC CCGACCCCGA GCACCCAGTC GCTGGACGCC GGCCCTGCTG 
GCCCTGCTGG CCCTGGGCGG CGCGGGACTG TGCCACGCGA GCTCGCAGCC TGGGTACCAC 
GCGAGGCCCA GTGCCAGGAA CAAAAACTGG TGCGCCTACA TCGTGAATAA GAACGTGAGC 
TGCACGGTAC AGGAGGGAAG CGAGAGTTTT ATTCAAGCTC AGTACAACTG TCCCTGGAAC 
CAGATGCCCT GTCCATCCGC CCTGGTGTAT CGGGTAAACT TTAGACCCAG ATTTGTCACT 
AGATACAAGA TAGTGACACA GTTGGAATGG AGATGCTGTC CTGGCTTTAG AGGACCAGAC 
TGCCAAGAAG GTCCCAAAGA CCACATGAAG ACCCCCCGGC CCCCATCAGC TCGACCAAAA 
AACAACCTGA AGAAAGCCAC AGATACAGAT CCAAGCCAAG TTTCTCAGCC TAAGAAAACG 
TTGTCCCCAA CAAATGCAGT GGAACCAGGT CAGGTTGCAG ATGCAAAGCA AGGCCCTCCG 
GAACTTCAGC AATCCAAAGT CCAGGTGCTG GAGGAGAAGG TCGTCCGACT CACCAGGATG 
GTGCTGGATC TCCAGTCCAC CGTGGTAGGC CTGAAGGAGA ACCTCAAACA CACCATTCAA 
GATGATGGCA GAAAAGAGCC AGACTCCTGG CTCGGTCCCC TGCACCCACA GCCAACGCCT 
GACAGCCCCC TTGCGGGGGA CGCAGAGCCG AGCCAGCTTC CCGGAATCCC CAGCAGCAAG 
GAATCTGGTA TGAAGGACAT CAAGTCTGAG TTGGCTGAGG TCAAAGACAC ACTGAAGACC 
AAGAGTGACA AGCTAGAAGA GCTGGACGGG AAGGTGAAGG GCTATGAAGG ACAGCTCAAG 
CAGCTGCAGG AGGCGGCTCA GGGTCCCACG GTCACCATGA CAACCAATGA GCTGTACCAA 
GCCTATGTGG ATAGCAAGAT CGATGCCCTG AGGGAGGAGC TCATGGAAGG CATGGACCGG 
AAGCTGGCTG ACCTGAAGAA TACGTGCGAG TACAAGCTGG TGGGGCTCCA GCAGCAGTGT 
GATGACTACG GCAGCAGCTA CCTCGGAGTG ATAGAGCTGA TCGGGGAGAA GGAGGCGAGC 
CTGAAAAAAG ACATTGCTGA CCTGCGAGCC CAGCTGCAGG ATCCTGTAGC CCAACCAAGC 
TGTTGCAATG GTCAAAAGAG CAGCGATTTC GGCCCACAGA TCAAGGCGCT AGACCAGAAA 
ATCGAGAGAG TTGCCGAGGC CACCAGGATG CTGAATGGAC GGCTGGACAA TGAGTTCGAC 
CGCCTCTCCG TTCCAGAGCC AGACGCAGAC TTTGATGCGA GATGGACCGA GCTCGACGCA 
AGGATCAATG TGACGGAGAA GAACGCAGAG GAGCATTGCT TTTACATTGA GGAGACCCTG 
CGGGGGACCA TCAACGGCGA AGTGGATGAC TTGAGGAAGC TTCTGAATGA GAAGATCCAC 
TCCCTGGAAG ACCGTCTGGG AATTGTTCTG CAAGCGGCTA ACAGCTCAGA CGTGGAGTTG 
ACACCCATGG GCCCAGCCTT GCCAGAGCAG CCAGGGGCAG AGAATGAGCA GGTCCTAATG 
GAGTTGAGCC GCCTGAAGGA CAAAGTTCAA GTGGTTGAGG ACTTCTGCCT GCAGAGCTTG 
CCCCACGGGA TAGATGGAGC TTTGCCTAGT GTAGAGGACC TCACGCATGT CAGCCTGAGC 
CTTTTAGAAT CCCTCAATGA TACGATGCAC AGGCAGTTCC AAGAAACGAG TCACAGCATC 
CAGAAACTCC AAGAGGACGT CAATGCCCTC CATTCTCAAC TGAATCACTC CGAATGTACG 
GGGACTTACT TGCAGAACGG AGTGAGCGAC AGCCGAACAG GTGACAGCAT GGAAGCAAGT 
GGGTTTACTA AGACAGGGGA GCAAGAGAGG ACAGTGGGGA CTGTCCCGTC TCCTGGGACC 
CCCGCGGCTC CATGCTGTGG TCAGCTGGAG GAGCGCTGGC AGAAGCTGCA GAACCAAATG 
TTGGCTGAGC TAGACACGTG TAAGGAGAGT GCTCACGGGG TTCAGAGTGG GGTGTCTGCG 
ATCGAGGGCA GGGTGTTCCA GCTGGAGCAG ACATGCAGAA GGCTGGATAC CATCTCAGGG 
AGTCTGCAGA GGATCAAGGA GGGGCTCGGT AAGCATGTGG GCAGCTTGTG GAACTGCATC 
CGGCAGATGA ACGGGACGCT CAAATCGCAC TCCAGAGACA TCTCTGGCCT CAAGAATTCT 
GTGCAGCAAT TCTATAGCCA TGTTTTCCAG ATTTCTACCG ACTTGCAAGA TCTGGTCAAG 
TTTCAGCCAT CAGCAACAGA GGAACCCTCA GAAGCAACCG AGGGACCGTC TGGGAAGACT 
CCCCTAGAAT CCACCAGACC TTCAGAGGAG GCCCCCACTG AACCACCCCG GCTGACCCCA 
CTGCCAGAAG ACCCTGCAGG ACCACCACAG ACAGGCCAGC AGCCAGTATT GCCCCAGAGG 
CCCCTGCAGC CACCACCTTT GCCTGCCTGG CCTGGTCGCA CGGGGTTGCC CTTCTTGCCT 
GGCTCTAGTG GCGTCATCAT GGAGACTGGA GAGGCGGGGC CTCCAGGCAG GATGGGCGTG 
TCCGGAAGGG GCCTGCCTCG GGGAGTGGAT GGCCAGATGG GGCAGGGGCC CATTCATAGC 
TCGGAAGGCT ATGCAGGGGC ACCAGGCTAC CCCAAGTCAC CTCCTGTAAC CACCCCAGGG 
GTGCCGCTGC CCACTCTGGT GTCCTTTTCA GCCGGTCTTA CCCAGAAGCC TTTTCCCAGC 
GATGGAGGCG TTGTTCTCTT CAATAAGGTG CTGGTGAACG ATGGGGATGT ATACAACCCC 
AACACTGGGA TCTTCACCGC ACCATACGAC GGGCGCTACT TGATCACAGC CACCCTCACC 
CCTGAGAGAG ACACCTATGT GGAAGCCGTC CTGTCTGTCT CCAACGCCAG TGTTGCCCAG 
CTGCACACGG CTGGGTACAG GAGAGAGTTC CTGGAATACC ATCGTCCCCC TGGGGCTGTG 
CATACCTGTG GGGGCCCGGG GGCCTTCCAC CTCATTGTGC ACTTGAAGGC AGGAGATGGA 
GTCAATGTTG TGGTGACTGG GGGCAGACTG GCTCACACAG ACTTTGATGA AATGTACTCC 
ACCTTCAGTG GTGTTTTCTT GTACCCTTTC CTTTCTCACC TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_660140.1
CDS	106..3330
Translation	MCQETPPRPRAPSRWTPALLALLALGGAGLCHASSQPGYHARPSARNKNWCAYIVNKNVSCTVQEGSESFIQAQYNCPWNQMPCPSALVYRVNFRPRFVTRYKIVTQLEWRCCPGFRGPDCQEGPKDHMKTPRPPSARPKNNLKKATDTDPSQVSQPKKTLSPTNAVEPGQVADAKQGPPELQQSKVQVLEEKVVRLTRMVLDLQSTVVGLKENLKHTIQDDGRKEPDSWLGPLHPQPTPDSPLAGDAEPSQLPGIPSSKESGMKDIKSELAEVKDTLKTKSDKLEELDGKVKGYEGQLKQLQEAAQGPTVTMTTNELYQAYVDSKIDALREELMEGMDRKLADLKNTCEYKLVGLQQQCDDYGSSYLGVIELIGEKEASLKKDIADLRAQLQDPVAQPSCCNGQKSSDFGPQIKALDQKIERVAEATRMLNGRLDNEFDRLSVPEPDADFDARWTELDARINVTEKNAEEHCFYIEETLRGTINGEVDDLRKLLNEKIHSLEDRLGIVLQAANSSDVELTPMGPALPEQPGAENEQVLMELSRLKDKVQVVEDFCLQSLPHGIDGALPSVEDLTHVSLSLLESLNDTMHRQFQETSHSIQKLQEDVNALHSQLNHSECTGTYLQNGVSDSRTGDSMEASGFTKTGEQERTVGTVPSPGTPAAPCCGQLEERWQKLQNQMLAELDTCKESAHGVQSGVSAIEGRVFQLEQTCRRLDTISGSLQRIKEGLGKHVGSLWNCIRQMNGTLKSHSRDISGLKNSVQQFYSHVFQISTDLQDLVKFQPSATEEPSEATEGPSGKTPLESTRPSEEAPTEPPRLTPLPEDPAGPPQTGQQPVLPQRPLQPPPLPAWPGRTGLPFLPGSSGVIMETGEAGPPGRMGVSGRGLPRGVDGQMGQGPIHSSEGYAGAPGYPKSPPVTTPGVPLPTLVSFSAGLTQKPFPSDGGVVLFNKVLVNDGDVYNPNTGIFTAPYDGRYLITATLTPERDTYVEAVLSVSNASVAQLHTAGYRREFLEYHRPPGAVHTCGGPGAFHLIVHLKAGDGVNVVVTGGRLAHTDFDEMYSTFSGVFLYPFLSHL

Target ORF information:

RefSeq Version	NM_145158.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus elastin microfibril interfacer 2 (Emilin2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145158.3

ORF Insert Sequence:

ATGTGTCAGG AGACCCCGCC CCGACCCCGA GCACCCAGTC GCTGGACGCC GGCCCTGCTG 
GCCCTGCTGG CCCTGGGCGG CGCGGGACTG TGCCACGCGA GCTCGCAGCC TGGGTACCAC 
GCGAGGCCCA GTGCCAGGAA CAAAAACTGG TGCGCCTACA TCGTGAATAA GAACGTGAGC 
TGCACGGTAC AGGAGGGAAG CGAGAGTTTT ATTCAAGCTC AGTACAACTG TCCCTGGAAC 
CAGATGCCCT GTCCATCCGC CCTGGTGTAT CGGGTAAACT TTAGACCCAG ATTTGTCACT 
AGATACAAGA TAGTGACACA GTTGGAATGG AGATGCTGTC CTGGCTTTAG AGGACCAGAC 
TGCCAAGAAG GTCCCAAAGA CCACATGAAG ACCCCCCGGC CCCCATCAGC TCGACCAAAA 
AACAACCTGA AGAAAGCCAC AGATACAGAT CCAAGCCAAG TTTCTCAGCC TAAGAAAACG 
TTGTCCCCAA CAAATGCAGT GGAACCAGGT CAGGTTGCAG ATGCAAAGCA AGGCCCTCCG 
GAACTTCAGC AATCCAAAGT CCAGGTGCTG GAGGAGAAGG TCGTCCGACT CACCAGGATG 
GTGCTGGATC TCCAGTCCAC CGTGGTAGGC CTGAAGGAGA ACCTCAAACA CACCATTCAA 
GATGATGGCA GAAAAGAGCC AGACTCCTGG CTCGGTCCCC TGCACCCACA GCCAACGCCT 
GACAGCCCCC TTGCGGGGGA CGCAGAGCCG AGCCAGCTTC CCGGAATCCC CAGCAGCAAG 
GAATCTGGTA TGAAGGACAT CAAGTCTGAG TTGGCTGAGG TCAAAGACAC ACTGAAGACC 
AAGAGTGACA AGCTAGAAGA GCTGGACGGG AAGGTGAAGG GCTATGAAGG ACAGCTCAAG 
CAGCTGCAGG AGGCGGCTCA GGGTCCCACG GTCACCATGA CAACCAATGA GCTGTACCAA 
GCCTATGTGG ATAGCAAGAT CGATGCCCTG AGGGAGGAGC TCATGGAAGG CATGGACCGG 
AAGCTGGCTG ACCTGAAGAA TACGTGCGAG TACAAGCTGG TGGGGCTCCA GCAGCAGTGT 
GATGACTACG GCAGCAGCTA CCTCGGAGTG ATAGAGCTGA TCGGGGAGAA GGAGGCGAGC 
CTGAAAAAAG ACATTGCTGA CCTGCGAGCC CAGCTGCAGG ATCCTGTAGC CCAACCAAGC 
TGTTGCAATG GTCAAAAGAG CAGCGATTTC GGCCCACAGA TCAAGGCGCT AGACCAGAAA 
ATCGAGAGAG TTGCCGAGGC CACCAGGATG CTGAATGGAC GGCTGGACAA TGAGTTCGAC 
CGCCTCTCCG TTCCAGAGCC AGACGCAGAC TTTGATGCGA GATGGACCGA GCTCGACGCA 
AGGATCAATG TGACGGAGAA GAACGCAGAG GAGCATTGCT TTTACATTGA GGAGACCCTG 
CGGGGGACCA TCAACGGCGA AGTGGATGAC TTGAGGAAGC TTCTGAATGA GAAGATCCAC 
TCCCTGGAAG ACCGTCTGGG AATTGTTCTG CAAGCGGCTA ACAGCTCAGA CGTGGAGTTG 
ACACCCATGG GCCCAGCCTT GCCAGAGCAG CCAGGGGCAG AGAATGAGCA GGTCCTAATG 
GAGTTGAGCC GCCTGAAGGA CAAAGTTCAA GTGGTTGAGG ACTTCTGCCT GCAGAGCTTG 
CCCCACGGGA TAGATGGAGC TTTGCCTAGT GTAGAGGACC TCACGCATGT CAGCCTGAGC 
CTTTTAGAAT CCCTCAATGA TACGATGCAC AGGCAGTTCC AAGAAACGAG TCACAGCATC 
CAGAAACTCC AAGAGGACGT CAATGCCCTC CATTCTCAAC TGAATCACTC CGAATGTACG 
GGGACTTACT TGCAGAACGG AGTGAGCGAC AGCCGAACAG GTGACAGCAT GGAAGCAAGT 
GGGTTTACTA AGACAGGGGA GCAAGAGAGG ACAGTGGGGA CTGTCCCGTC TCCTGGGACC 
CCCGCGGCTC CATGCTGTGG TCAGCTGGAG GAGCGCTGGC AGAAGCTGCA GAACCAAATG 
TTGGCTGAGC TAGACACGTG TAAGGAGAGT GCTCACGGGG TTCAGAGTGG GGTGTCTGCG 
ATCGAGGGCA GGGTGTTCCA GCTGGAGCAG ACATGCAGAA GGCTGGATAC CATCTCAGGG 
AGTCTGCAGA GGATCAAGGA GGGGCTCGGT AAGCATGTGG GCAGCTTGTG GAACTGCATC 
CGGCAGATGA ACGGGACGCT CAAATCGCAC TCCAGAGACA TCTCTGGCCT CAAGAATTCT 
GTGCAGCAAT TCTATAGCCA TGTTTTCCAG ATTTCTACCG ACTTGCAAGA TCTGGTCAAG 
TTTCAGCCAT CAGCAACAGA GGAACCCTCA GAAGCAACCG AGGGACCGTC TGGGAAGACT 
CCCCTAGAAT CCACCAGACC TTCAGAGGAG GCCCCCACTG AACCACCCCG GCTGACCCCA 
CTGCCAGAAG ACCCTGCAGG ACCACCACAG ACAGGCCAGC AGCCAGTATT GCCCCAGAGG 
CCCCTGCAGC CACCACCTTT GCCTGCCTGG CCTGGTCGCA CGGGGTTGCC CTTCTTGCCT 
GGCTCTAGTG GCGTCATCAT GGAGACTGGA GAGGCGGGGC CTCCAGGCAG GATGGGCGTG 
TCCGGAAGGG GCCTGCCTCG GGGAGTGGAT GGCCAGATGG GGCAGGGGCC CATTCATAGC 
TCGGAAGGCT ATGCAGGGGC ACCAGGCTAC CCCAAGTCAC CTCCTGTAAC CACCCCAGGG 
GTGCCGCTGC CCACTCTGGT GTCCTTTTCA GCCGGTCTTA CCCAGAAGCC TTTTCCCAGC 
GATGGAGGCG TTGTTCTCTT CAATAAGGTG CTGGTGAACG ATGGGGATGT ATACAACCCC 
AACACTGGGA TCTTCACCGC ACCATACGAC GGGCGCTACT TGATCACAGC CACCCTCACC 
CCTGAGAGAG ACACCTATGT GGAAGCCGTC CTGTCTGTCT CCAACGCCAG TGTTGCCCAG 
CTGCACACGG CTGGGTACAG GAGAGAGTTC CTGGAATACC ATCGTCCCCC TGGGGCTGTG 
CATACCTGTG GGGGCCCGGG GGCCTTCCAC CTCATTGTGC ACTTGAAGGC AGGAGATGGA 
GTCAATGTTG TGGTGACTGG GGGCAGACTG GCTCACACAG ACTTTGATGA AATGTACTCC 
ACCTTCAGTG GTGTTTTCTT GTACCCTTTC CTTTCTCACC TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Emilin2 ( NM_145158.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_660140.1 Mus musculus elastin microfibril interfacer 2 (Emilin2), transcript variant 1, mRNA.

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CloneID	OMu11369
Clone ID Related Accession (Same CDS sequence)	NM_145158.3 , NM_145158.4
Accession Version	NM_145158.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	EMILIN-2 isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY232991.1, AK155909.1 and BY582438.1. On Oct 26, 2017 this sequence version replaced NM_145158.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK155918.1, AK171015.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164135 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGTCAGG AGACCCCGCC CCGACCCCGA GCACCCAGTC GCTGGACGCC GGCCCTGCTG 
GCCCTGCTGG CCCTGGGCGG CGCGGGACTG TGCCACGCGA GCTCGCAGCC TGGGTACCAC 
GCGAGGCCCA GTGCCAGGAA CAAAAACTGG TGCGCCTACA TCGTGAATAA GAACGTGAGC 
TGCACGGTAC AGGAGGGAAG CGAGAGTTTT ATTCAAGCTC AGTACAACTG TCCCTGGAAC 
CAGATGCCCT GTCCATCCGC CCTGGTGTAT CGGGTAAACT TTAGACCCAG ATTTGTCACT 
AGATACAAGA TAGTGACACA GTTGGAATGG AGATGCTGTC CTGGCTTTAG AGGACCAGAC 
TGCCAAGAAG GTCCCAAAGA CCACATGAAG ACCCCCCGGC CCCCATCAGC TCGACCAAAA 
AACAACCTGA AGAAAGCCAC AGATACAGAT CCAAGCCAAG TTTCTCAGCC TAAGAAAACG 
TTGTCCCCAA CAAATGCAGT GGAACCAGGT CAGGTTGCAG ATGCAAAGCA AGGCCCTCCG 
GAACTTCAGC AATCCAAAGT CCAGGTGCTG GAGGAGAAGG TCGTCCGACT CACCAGGATG 
GTGCTGGATC TCCAGTCCAC CGTGGTAGGC CTGAAGGAGA ACCTCAAACA CACCATTCAA 
GATGATGGCA GAAAAGAGCC AGACTCCTGG CTCGGTCCCC TGCACCCACA GCCAACGCCT 
GACAGCCCCC TTGCGGGGGA CGCAGAGCCG AGCCAGCTTC CCGGAATCCC CAGCAGCAAG 
GAATCTGGTA TGAAGGACAT CAAGTCTGAG TTGGCTGAGG TCAAAGACAC ACTGAAGACC 
AAGAGTGACA AGCTAGAAGA GCTGGACGGG AAGGTGAAGG GCTATGAAGG ACAGCTCAAG 
CAGCTGCAGG AGGCGGCTCA GGGTCCCACG GTCACCATGA CAACCAATGA GCTGTACCAA 
GCCTATGTGG ATAGCAAGAT CGATGCCCTG AGGGAGGAGC TCATGGAAGG CATGGACCGG 
AAGCTGGCTG ACCTGAAGAA TACGTGCGAG TACAAGCTGG TGGGGCTCCA GCAGCAGTGT 
GATGACTACG GCAGCAGCTA CCTCGGAGTG ATAGAGCTGA TCGGGGAGAA GGAGGCGAGC 
CTGAAAAAAG ACATTGCTGA CCTGCGAGCC CAGCTGCAGG ATCCTGTAGC CCAACCAAGC 
TGTTGCAATG GTCAAAAGAG CAGCGATTTC GGCCCACAGA TCAAGGCGCT AGACCAGAAA 
ATCGAGAGAG TTGCCGAGGC CACCAGGATG CTGAATGGAC GGCTGGACAA TGAGTTCGAC 
CGCCTCTCCG TTCCAGAGCC AGACGCAGAC TTTGATGCGA GATGGACCGA GCTCGACGCA 
AGGATCAATG TGACGGAGAA GAACGCAGAG GAGCATTGCT TTTACATTGA GGAGACCCTG 
CGGGGGACCA TCAACGGCGA AGTGGATGAC TTGAGGAAGC TTCTGAATGA GAAGATCCAC 
TCCCTGGAAG ACCGTCTGGG AATTGTTCTG CAAGCGGCTA ACAGCTCAGA CGTGGAGTTG 
ACACCCATGG GCCCAGCCTT GCCAGAGCAG CCAGGGGCAG AGAATGAGCA GGTCCTAATG 
GAGTTGAGCC GCCTGAAGGA CAAAGTTCAA GTGGTTGAGG ACTTCTGCCT GCAGAGCTTG 
CCCCACGGGA TAGATGGAGC TTTGCCTAGT GTAGAGGACC TCACGCATGT CAGCCTGAGC 
CTTTTAGAAT CCCTCAATGA TACGATGCAC AGGCAGTTCC AAGAAACGAG TCACAGCATC 
CAGAAACTCC AAGAGGACGT CAATGCCCTC CATTCTCAAC TGAATCACTC CGAATGTACG 
GGGACTTACT TGCAGAACGG AGTGAGCGAC AGCCGAACAG GTGACAGCAT GGAAGCAAGT 
GGGTTTACTA AGACAGGGGA GCAAGAGAGG ACAGTGGGGA CTGTCCCGTC TCCTGGGACC 
CCCGCGGCTC CATGCTGTGG TCAGCTGGAG GAGCGCTGGC AGAAGCTGCA GAACCAAATG 
TTGGCTGAGC TAGACACGTG TAAGGAGAGT GCTCACGGGG TTCAGAGTGG GGTGTCTGCG 
ATCGAGGGCA GGGTGTTCCA GCTGGAGCAG ACATGCAGAA GGCTGGATAC CATCTCAGGG 
AGTCTGCAGA GGATCAAGGA GGGGCTCGGT AAGCATGTGG GCAGCTTGTG GAACTGCATC 
CGGCAGATGA ACGGGACGCT CAAATCGCAC TCCAGAGACA TCTCTGGCCT CAAGAATTCT 
GTGCAGCAAT TCTATAGCCA TGTTTTCCAG ATTTCTACCG ACTTGCAAGA TCTGGTCAAG 
TTTCAGCCAT CAGCAACAGA GGAACCCTCA GAAGCAACCG AGGGACCGTC TGGGAAGACT 
CCCCTAGAAT CCACCAGACC TTCAGAGGAG GCCCCCACTG AACCACCCCG GCTGACCCCA 
CTGCCAGAAG ACCCTGCAGG ACCACCACAG ACAGGCCAGC AGCCAGTATT GCCCCAGAGG 
CCCCTGCAGC CACCACCTTT GCCTGCCTGG CCTGGTCGCA CGGGGTTGCC CTTCTTGCCT 
GGCTCTAGTG GCGTCATCAT GGAGACTGGA GAGGCGGGGC CTCCAGGCAG GATGGGCGTG 
TCCGGAAGGG GCCTGCCTCG GGGAGTGGAT GGCCAGATGG GGCAGGGGCC CATTCATAGC 
TCGGAAGGCT ATGCAGGGGC ACCAGGCTAC CCCAAGTCAC CTCCTGTAAC CACCCCAGGG 
GTGCCGCTGC CCACTCTGGT GTCCTTTTCA GCCGGTCTTA CCCAGAAGCC TTTTCCCAGC 
GATGGAGGCG TTGTTCTCTT CAATAAGGTG CTGGTGAACG ATGGGGATGT ATACAACCCC 
AACACTGGGA TCTTCACCGC ACCATACGAC GGGCGCTACT TGATCACAGC CACCCTCACC 
CCTGAGAGAG ACACCTATGT GGAAGCCGTC CTGTCTGTCT CCAACGCCAG TGTTGCCCAG 
CTGCACACGG CTGGGTACAG GAGAGAGTTC CTGGAATACC ATCGTCCCCC TGGGGCTGTG 
CATACCTGTG GGGGCCCGGG GGCCTTCCAC CTCATTGTGC ACTTGAAGGC AGGAGATGGA 
GTCAATGTTG TGGTGACTGG GGGCAGACTG GCTCACACAG ACTTTGATGA AATGTACTCC 
ACCTTCAGTG GTGTTTTCTT GTACCCTTTC CTTTCTCACC TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_660140.1
CDS	152..3376
Translation	MCQETPPRPRAPSRWTPALLALLALGGAGLCHASSQPGYHARPSARNKNWCAYIVNKNVSCTVQEGSESFIQAQYNCPWNQMPCPSALVYRVNFRPRFVTRYKIVTQLEWRCCPGFRGPDCQEGPKDHMKTPRPPSARPKNNLKKATDTDPSQVSQPKKTLSPTNAVEPGQVADAKQGPPELQQSKVQVLEEKVVRLTRMVLDLQSTVVGLKENLKHTIQDDGRKEPDSWLGPLHPQPTPDSPLAGDAEPSQLPGIPSSKESGMKDIKSELAEVKDTLKTKSDKLEELDGKVKGYEGQLKQLQEAAQGPTVTMTTNELYQAYVDSKIDALREELMEGMDRKLADLKNTCEYKLVGLQQQCDDYGSSYLGVIELIGEKEASLKKDIADLRAQLQDPVAQPSCCNGQKSSDFGPQIKALDQKIERVAEATRMLNGRLDNEFDRLSVPEPDADFDARWTELDARINVTEKNAEEHCFYIEETLRGTINGEVDDLRKLLNEKIHSLEDRLGIVLQAANSSDVELTPMGPALPEQPGAENEQVLMELSRLKDKVQVVEDFCLQSLPHGIDGALPSVEDLTHVSLSLLESLNDTMHRQFQETSHSIQKLQEDVNALHSQLNHSECTGTYLQNGVSDSRTGDSMEASGFTKTGEQERTVGTVPSPGTPAAPCCGQLEERWQKLQNQMLAELDTCKESAHGVQSGVSAIEGRVFQLEQTCRRLDTISGSLQRIKEGLGKHVGSLWNCIRQMNGTLKSHSRDISGLKNSVQQFYSHVFQISTDLQDLVKFQPSATEEPSEATEGPSGKTPLESTRPSEEAPTEPPRLTPLPEDPAGPPQTGQQPVLPQRPLQPPPLPAWPGRTGLPFLPGSSGVIMETGEAGPPGRMGVSGRGLPRGVDGQMGQGPIHSSEGYAGAPGYPKSPPVTTPGVPLPTLVSFSAGLTQKPFPSDGGVVLFNKVLVNDGDVYNPNTGIFTAPYDGRYLITATLTPERDTYVEAVLSVSNASVAQLHTAGYRREFLEYHRPPGAVHTCGGPGAFHLIVHLKAGDGVNVVVTGGRLAHTDFDEMYSTFSGVFLYPFLSHL

Target ORF information:

RefSeq Version	NM_145158.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus elastin microfibril interfacer 2 (Emilin2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145158.4

ORF Insert Sequence:

ATGTGTCAGG AGACCCCGCC CCGACCCCGA GCACCCAGTC GCTGGACGCC GGCCCTGCTG 
GCCCTGCTGG CCCTGGGCGG CGCGGGACTG TGCCACGCGA GCTCGCAGCC TGGGTACCAC 
GCGAGGCCCA GTGCCAGGAA CAAAAACTGG TGCGCCTACA TCGTGAATAA GAACGTGAGC 
TGCACGGTAC AGGAGGGAAG CGAGAGTTTT ATTCAAGCTC AGTACAACTG TCCCTGGAAC 
CAGATGCCCT GTCCATCCGC CCTGGTGTAT CGGGTAAACT TTAGACCCAG ATTTGTCACT 
AGATACAAGA TAGTGACACA GTTGGAATGG AGATGCTGTC CTGGCTTTAG AGGACCAGAC 
TGCCAAGAAG GTCCCAAAGA CCACATGAAG ACCCCCCGGC CCCCATCAGC TCGACCAAAA 
AACAACCTGA AGAAAGCCAC AGATACAGAT CCAAGCCAAG TTTCTCAGCC TAAGAAAACG 
TTGTCCCCAA CAAATGCAGT GGAACCAGGT CAGGTTGCAG ATGCAAAGCA AGGCCCTCCG 
GAACTTCAGC AATCCAAAGT CCAGGTGCTG GAGGAGAAGG TCGTCCGACT CACCAGGATG 
GTGCTGGATC TCCAGTCCAC CGTGGTAGGC CTGAAGGAGA ACCTCAAACA CACCATTCAA 
GATGATGGCA GAAAAGAGCC AGACTCCTGG CTCGGTCCCC TGCACCCACA GCCAACGCCT 
GACAGCCCCC TTGCGGGGGA CGCAGAGCCG AGCCAGCTTC CCGGAATCCC CAGCAGCAAG 
GAATCTGGTA TGAAGGACAT CAAGTCTGAG TTGGCTGAGG TCAAAGACAC ACTGAAGACC 
AAGAGTGACA AGCTAGAAGA GCTGGACGGG AAGGTGAAGG GCTATGAAGG ACAGCTCAAG 
CAGCTGCAGG AGGCGGCTCA GGGTCCCACG GTCACCATGA CAACCAATGA GCTGTACCAA 
GCCTATGTGG ATAGCAAGAT CGATGCCCTG AGGGAGGAGC TCATGGAAGG CATGGACCGG 
AAGCTGGCTG ACCTGAAGAA TACGTGCGAG TACAAGCTGG TGGGGCTCCA GCAGCAGTGT 
GATGACTACG GCAGCAGCTA CCTCGGAGTG ATAGAGCTGA TCGGGGAGAA GGAGGCGAGC 
CTGAAAAAAG ACATTGCTGA CCTGCGAGCC CAGCTGCAGG ATCCTGTAGC CCAACCAAGC 
TGTTGCAATG GTCAAAAGAG CAGCGATTTC GGCCCACAGA TCAAGGCGCT AGACCAGAAA 
ATCGAGAGAG TTGCCGAGGC CACCAGGATG CTGAATGGAC GGCTGGACAA TGAGTTCGAC 
CGCCTCTCCG TTCCAGAGCC AGACGCAGAC TTTGATGCGA GATGGACCGA GCTCGACGCA 
AGGATCAATG TGACGGAGAA GAACGCAGAG GAGCATTGCT TTTACATTGA GGAGACCCTG 
CGGGGGACCA TCAACGGCGA AGTGGATGAC TTGAGGAAGC TTCTGAATGA GAAGATCCAC 
TCCCTGGAAG ACCGTCTGGG AATTGTTCTG CAAGCGGCTA ACAGCTCAGA CGTGGAGTTG 
ACACCCATGG GCCCAGCCTT GCCAGAGCAG CCAGGGGCAG AGAATGAGCA GGTCCTAATG 
GAGTTGAGCC GCCTGAAGGA CAAAGTTCAA GTGGTTGAGG ACTTCTGCCT GCAGAGCTTG 
CCCCACGGGA TAGATGGAGC TTTGCCTAGT GTAGAGGACC TCACGCATGT CAGCCTGAGC 
CTTTTAGAAT CCCTCAATGA TACGATGCAC AGGCAGTTCC AAGAAACGAG TCACAGCATC 
CAGAAACTCC AAGAGGACGT CAATGCCCTC CATTCTCAAC TGAATCACTC CGAATGTACG 
GGGACTTACT TGCAGAACGG AGTGAGCGAC AGCCGAACAG GTGACAGCAT GGAAGCAAGT 
GGGTTTACTA AGACAGGGGA GCAAGAGAGG ACAGTGGGGA CTGTCCCGTC TCCTGGGACC 
CCCGCGGCTC CATGCTGTGG TCAGCTGGAG GAGCGCTGGC AGAAGCTGCA GAACCAAATG 
TTGGCTGAGC TAGACACGTG TAAGGAGAGT GCTCACGGGG TTCAGAGTGG GGTGTCTGCG 
ATCGAGGGCA GGGTGTTCCA GCTGGAGCAG ACATGCAGAA GGCTGGATAC CATCTCAGGG 
AGTCTGCAGA GGATCAAGGA GGGGCTCGGT AAGCATGTGG GCAGCTTGTG GAACTGCATC 
CGGCAGATGA ACGGGACGCT CAAATCGCAC TCCAGAGACA TCTCTGGCCT CAAGAATTCT 
GTGCAGCAAT TCTATAGCCA TGTTTTCCAG ATTTCTACCG ACTTGCAAGA TCTGGTCAAG 
TTTCAGCCAT CAGCAACAGA GGAACCCTCA GAAGCAACCG AGGGACCGTC TGGGAAGACT 
CCCCTAGAAT CCACCAGACC TTCAGAGGAG GCCCCCACTG AACCACCCCG GCTGACCCCA 
CTGCCAGAAG ACCCTGCAGG ACCACCACAG ACAGGCCAGC AGCCAGTATT GCCCCAGAGG 
CCCCTGCAGC CACCACCTTT GCCTGCCTGG CCTGGTCGCA CGGGGTTGCC CTTCTTGCCT 
GGCTCTAGTG GCGTCATCAT GGAGACTGGA GAGGCGGGGC CTCCAGGCAG GATGGGCGTG 
TCCGGAAGGG GCCTGCCTCG GGGAGTGGAT GGCCAGATGG GGCAGGGGCC CATTCATAGC 
TCGGAAGGCT ATGCAGGGGC ACCAGGCTAC CCCAAGTCAC CTCCTGTAAC CACCCCAGGG 
GTGCCGCTGC CCACTCTGGT GTCCTTTTCA GCCGGTCTTA CCCAGAAGCC TTTTCCCAGC 
GATGGAGGCG TTGTTCTCTT CAATAAGGTG CTGGTGAACG ATGGGGATGT ATACAACCCC 
AACACTGGGA TCTTCACCGC ACCATACGAC GGGCGCTACT TGATCACAGC CACCCTCACC 
CCTGAGAGAG ACACCTATGT GGAAGCCGTC CTGTCTGTCT CCAACGCCAG TGTTGCCCAG 
CTGCACACGG CTGGGTACAG GAGAGAGTTC CTGGAATACC ATCGTCCCCC TGGGGCTGTG 
CATACCTGTG GGGGCCCGGG GGCCTTCCAC CTCATTGTGC ACTTGAAGGC AGGAGATGGA 
GTCAATGTTG TGGTGACTGG GGGCAGACTG GCTCACACAG ACTTTGATGA AATGTACTCC 
ACCTTCAGTG GTGTTTTCTT GTACCCTTTC CTTTCTCACC TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Emilin2 ( NM_001357337.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001344266.1 Mus musculus elastin microfibril interfacer 2 (Emilin2), transcript variant 3, mRNA.

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CloneID	OMu53594
Clone ID Related Accession (Same CDS sequence)	XM_006524285.3 , NM_001357337.1
Accession Version	NM_001357337.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	EMILIN-2 isoform 3 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC126942.4 and BY582438.1. On Oct 26, 2017 this sequence version replaced XM_006524285.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK170081.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGTCAGG AGACCCCGCC CCGACCCCGA GCACCCAGTC GCTGGACGCC GGCCCTGCTG 
GCCCTGCTGG CCCTGGGCGG CGCGGGACTG TGCCACGCGA GCTCGCAGCC TGGGTACCAC 
GCGAGGCCCA GTGCCAGGAA CAAAAACTGG TGCGCCTACA TCGTGAATAA GAACGTGAGC 
TGCACGGTAC AGGAGGGAAG CGAGAGTTTT ATTCAAGCTC AGTACAACTG TCCCTGGAAC 
CAGATGCCCT GTCCATCCGC CCTGGTGTAT CGGGTAAACT TTAGACCCAG ATTTGTCACT 
AGATACAAGA TAGTGACACA GTTGGAATGG AGATGCTGTC CTGGCTTTAG AGGACCAGAC 
TGCCAAGAAG GTCCCAAAGA CCACATGAAG ACCCCCCGGC CCCCATCAGC TCGACCAAAA 
AACAACCTGA AGAAAGCCAC AGATACAGAT CCAAGCCAAG TTTCTCAGCC TAAGAAAACG 
TTGTCCCCAA CAAATGCAGT GGAACCAGGT CAGGTTGCAG ATGCAAAGCA AGGCCCTCCG 
GAACTTCAGC AATCCAAAGT CCAGGTGCTG GAGGAGAAGG TCGTCCGACT CACCAGGATG 
GTGCTGGATC TCCAGTCCAC CGTGGTAGGC CTGAAGGAGA ACCTCAAACA CACCATTCAA 
GATGATGGCA GAAAAGAGCC AGACTCCTGG CTCGGTCCCC TGCACCCACA GCCAACGCCT 
GACAGCCCCC TTGCGGGGGA CGCAGAGCCG AGCCAGCTTC CCGGAATCCC CAGCAGCAAG 
GAATCTGGTA TGAAGGACAT CAAGTCTGAG TTGGCTGAGG TCAAAGACAC ACTGAAGACC 
AAGAGTGACA AGCTAGAAGA GCTGGACGGG AAGGTGAAGG GCTATGAAGG ACAGCTCAAG 
CAGCTGCAGG AGGCGGCTCA GGGTCCCACG GTCACCATGA CAACCAATGA GCTGTACCAA 
GCCTATGTGG ATAGCAAGAT CGATGCCCTG AGGGAGGAGC TCATGGAAGG CATGGACCGG 
AAGCTGGCTG ACCTGAAGAA TACGTGCGAG TACAAGCTGG TGGGGCTCCA GCAGCAGTGT 
GATGACTACG GCAGCAGCTA CCTCGGAGTG ATAGAGCTGA TCGGGGAGAA GGAGGCGAGC 
CTGAAAAAAG ACATTGCTGA CCTGCGAGCC CAGCTGCAGG ATCCTGTAGC CCAACCAAGC 
TGTTGCAATG GTCAAAAGAG CAGCGATTTC GGCCCACAGA TCAAGGCGCT AGACCAGAAA 
ATCGAGAGAG TTGCCGAGGC CACCAGGATG CTGAATGGAC GGCTGGACAA TGAGTTCGAC 
CGCCTCTCCG TTCCAGAGCC AGACGCAGAC TTTGATGCGA GATGGACCGA GCTCGACGCA 
AGGATCAATG TGACGGAGAA GAACGCAGAG GAGCATTGCT TTTACATTGA GGAGACCCTG 
CGGGGGACCA TCAACGGCGA AGTGGATGAC TTGAGGAAGC TTCTGAATGA GAAGATCCAC 
TCCCTGGAAG ACCGTCTGGG AATTGTTCTG CAAGCGGCTA ACAGCTCAGA CGTGGAGTTG 
ACACCCATGG GCCCAGCCTT GCCAGAGCAG CCAGGGGCAG AGAATGAGCA GGTCCTAATG 
GAGTTGAGCC GCCTGAAGGA CAAAGTTCAA GTGGTTGAGG ACTTCTGCCT GCAGAGCTTG 
CCCCACGGGA TAGATGGAGC TTTGCCTAGT GTAGAGGACC TCACGCATGT CAGCCTGAGC 
CTTTTAGAAT CCCTCAATGA TACGATGCAC AGGCAGTTCC AAGAAACGAG TCACAGCATC 
CAGAAACTCC AAGAGGACGT CAATGCCCTC CATTCTCAAC TGAATCACTC CGAATGTACG 
GGGACTTACT TGCAGAACGG AGTGAGCGAC AGCCGAACAG GTGACAGCAT GGAAGCAAGT 
GGGTTTACTA AGACAGGGGA GCAAGAGAGG ACAGTGGGGA CTGTCCCGTC TCCTGGGACC 
CCCGCGGCTC CATGCTGTGG TCAGCTGGAG GAGCGCTGGC AGAAGCTGCA GAACCAAATG 
TTGGCTGAGC TAGACACGTG TAAGGAGAGT GCTCACGGGG TTCAGAGTGG GGTGTCTGCG 
ATCGAGGGCA GGGTGTTCCA GCTGGAGCAG ACATGCAGAA GGCTGGATAC CATCTCAGGG 
AGTCTGCAGA GGATCAAGGA GGGGCTCGGT AAGCATGTGG GCAGCTTGTG GAACTGCATC 
CGGCAGATGA ACGGGACGCT CAAATCGCAC TCCAGAGACA TCTCTGGCCT CAAGAATTCT 
GTGCAGCAAT TCTATAGCCA TGTTTTCCAG ATTTCTACCG ACTTGCAAGA TCTGGTCAAG 
TTTCAGCCAT CAGCAAAGGA ACCCTCAGAA GCAACCGAGG GACCGTCTGG GAAGACTCCC 
CTAGAATCCA CCAGACCTTC AGAGGAGGCC CCCACTGAAC CACCCCGGCT GACCCCACTG 
CCAGAAGACC CTGCAGGACC ACCACAGACA GGCCAGCAGC CAGTATTGCC CCAGAGGCCC 
CTGCAGCCAC CACCTTTGCC TGCCTGGCCT GGTCGCACGG GGTTGCCCTT CTTGCCTGGC 
TCTAGTGGCG TCATCATGGA GACTGGAGAG GCGGGGCCTC CAGGCAGGAT GGGCGTGTCC 
GGAAGGGGCC TGCCTCGGGG AGTGGATGGC CAGATGGGGC AGGGGCCCAT TCATAGCTCG 
GAAGGCTATG CAGGGGCACC AGGCTACCCC AAGTCACCTC CTGTAACCAC CCCAGGGGTG 
CCGCTGCCCA CTCTGGTGTC CTTTTCAGCC GGTCTTACCC AGAAGCCTTT TCCCAGCGAT 
GGAGGCGTTG TTCTCTTCAA TAAGGTGCTG GTGAACGATG GGGATGTATA CAACCCCAAC 
ACTGGGATCT TCACCGCACC ATACGACGGG CGCTACTTGA TCACAGCCAC CCTCACCCCT 
GAGAGAGACA CCTATGTGGA AGCCGTCCTG TCTGTCTCCA ACGCCAGTGT TGCCCAGCTG 
CACACGGCTG GGTACAGGAG AGAGTTCCTG GAATACCATC GTCCCCCTGG GGCTGTGCAT 
ACCTGTGGGG GCCCGGGGGC CTTCCACCTC ATTGTGCACT TGAAGGCAGG AGATGGAGTC 
AATGTTGTGG TGACTGGGGG CAGACTGGCT CACACAGACT TTGATGAAAT GTACTCCACC 
TTCAGTGGTG TTTTCTTGTA CCCTTTCCTT TCTCACCTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001344266.1
CDS	152..3373
Translation	MCQETPPRPRAPSRWTPALLALLALGGAGLCHASSQPGYHARPSARNKNWCAYIVNKNVSCTVQEGSESFIQAQYNCPWNQMPCPSALVYRVNFRPRFVTRYKIVTQLEWRCCPGFRGPDCQEGPKDHMKTPRPPSARPKNNLKKATDTDPSQVSQPKKTLSPTNAVEPGQVADAKQGPPELQQSKVQVLEEKVVRLTRMVLDLQSTVVGLKENLKHTIQDDGRKEPDSWLGPLHPQPTPDSPLAGDAEPSQLPGIPSSKESGMKDIKSELAEVKDTLKTKSDKLEELDGKVKGYEGQLKQLQEAAQGPTVTMTTNELYQAYVDSKIDALREELMEGMDRKLADLKNTCEYKLVGLQQQCDDYGSSYLGVIELIGEKEASLKKDIADLRAQLQDPVAQPSCCNGQKSSDFGPQIKALDQKIERVAEATRMLNGRLDNEFDRLSVPEPDADFDARWTELDARINVTEKNAEEHCFYIEETLRGTINGEVDDLRKLLNEKIHSLEDRLGIVLQAANSSDVELTPMGPALPEQPGAENEQVLMELSRLKDKVQVVEDFCLQSLPHGIDGALPSVEDLTHVSLSLLESLNDTMHRQFQETSHSIQKLQEDVNALHSQLNHSECTGTYLQNGVSDSRTGDSMEASGFTKTGEQERTVGTVPSPGTPAAPCCGQLEERWQKLQNQMLAELDTCKESAHGVQSGVSAIEGRVFQLEQTCRRLDTISGSLQRIKEGLGKHVGSLWNCIRQMNGTLKSHSRDISGLKNSVQQFYSHVFQISTDLQDLVKFQPSAKEPSEATEGPSGKTPLESTRPSEEAPTEPPRLTPLPEDPAGPPQTGQQPVLPQRPLQPPPLPAWPGRTGLPFLPGSSGVIMETGEAGPPGRMGVSGRGLPRGVDGQMGQGPIHSSEGYAGAPGYPKSPPVTTPGVPLPTLVSFSAGLTQKPFPSDGGVVLFNKVLVNDGDVYNPNTGIFTAPYDGRYLITATLTPERDTYVEAVLSVSNASVAQLHTAGYRREFLEYHRPPGAVHTCGGPGAFHLIVHLKAGDGVNVVVTGGRLAHTDFDEMYSTFSGVFLYPFLSHL

Target ORF information:

RefSeq Version	NM_001357337.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus elastin microfibril interfacer 2 (Emilin2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001357337.1

ORF Insert Sequence:

ATGTGTCAGG AGACCCCGCC CCGACCCCGA GCACCCAGTC GCTGGACGCC GGCCCTGCTG 
GCCCTGCTGG CCCTGGGCGG CGCGGGACTG TGCCACGCGA GCTCGCAGCC TGGGTACCAC 
GCGAGGCCCA GTGCCAGGAA CAAAAACTGG TGCGCCTACA TCGTGAATAA GAACGTGAGC 
TGCACGGTAC AGGAGGGAAG CGAGAGTTTT ATTCAAGCTC AGTACAACTG TCCCTGGAAC 
CAGATGCCCT GTCCATCCGC CCTGGTGTAT CGGGTAAACT TTAGACCCAG ATTTGTCACT 
AGATACAAGA TAGTGACACA GTTGGAATGG AGATGCTGTC CTGGCTTTAG AGGACCAGAC 
TGCCAAGAAG GTCCCAAAGA CCACATGAAG ACCCCCCGGC CCCCATCAGC TCGACCAAAA 
AACAACCTGA AGAAAGCCAC AGATACAGAT CCAAGCCAAG TTTCTCAGCC TAAGAAAACG 
TTGTCCCCAA CAAATGCAGT GGAACCAGGT CAGGTTGCAG ATGCAAAGCA AGGCCCTCCG 
GAACTTCAGC AATCCAAAGT CCAGGTGCTG GAGGAGAAGG TCGTCCGACT CACCAGGATG 
GTGCTGGATC TCCAGTCCAC CGTGGTAGGC CTGAAGGAGA ACCTCAAACA CACCATTCAA 
GATGATGGCA GAAAAGAGCC AGACTCCTGG CTCGGTCCCC TGCACCCACA GCCAACGCCT 
GACAGCCCCC TTGCGGGGGA CGCAGAGCCG AGCCAGCTTC CCGGAATCCC CAGCAGCAAG 
GAATCTGGTA TGAAGGACAT CAAGTCTGAG TTGGCTGAGG TCAAAGACAC ACTGAAGACC 
AAGAGTGACA AGCTAGAAGA GCTGGACGGG AAGGTGAAGG GCTATGAAGG ACAGCTCAAG 
CAGCTGCAGG AGGCGGCTCA GGGTCCCACG GTCACCATGA CAACCAATGA GCTGTACCAA 
GCCTATGTGG ATAGCAAGAT CGATGCCCTG AGGGAGGAGC TCATGGAAGG CATGGACCGG 
AAGCTGGCTG ACCTGAAGAA TACGTGCGAG TACAAGCTGG TGGGGCTCCA GCAGCAGTGT 
GATGACTACG GCAGCAGCTA CCTCGGAGTG ATAGAGCTGA TCGGGGAGAA GGAGGCGAGC 
CTGAAAAAAG ACATTGCTGA CCTGCGAGCC CAGCTGCAGG ATCCTGTAGC CCAACCAAGC 
TGTTGCAATG GTCAAAAGAG CAGCGATTTC GGCCCACAGA TCAAGGCGCT AGACCAGAAA 
ATCGAGAGAG TTGCCGAGGC CACCAGGATG CTGAATGGAC GGCTGGACAA TGAGTTCGAC 
CGCCTCTCCG TTCCAGAGCC AGACGCAGAC TTTGATGCGA GATGGACCGA GCTCGACGCA 
AGGATCAATG TGACGGAGAA GAACGCAGAG GAGCATTGCT TTTACATTGA GGAGACCCTG 
CGGGGGACCA TCAACGGCGA AGTGGATGAC TTGAGGAAGC TTCTGAATGA GAAGATCCAC 
TCCCTGGAAG ACCGTCTGGG AATTGTTCTG CAAGCGGCTA ACAGCTCAGA CGTGGAGTTG 
ACACCCATGG GCCCAGCCTT GCCAGAGCAG CCAGGGGCAG AGAATGAGCA GGTCCTAATG 
GAGTTGAGCC GCCTGAAGGA CAAAGTTCAA GTGGTTGAGG ACTTCTGCCT GCAGAGCTTG 
CCCCACGGGA TAGATGGAGC TTTGCCTAGT GTAGAGGACC TCACGCATGT CAGCCTGAGC 
CTTTTAGAAT CCCTCAATGA TACGATGCAC AGGCAGTTCC AAGAAACGAG TCACAGCATC 
CAGAAACTCC AAGAGGACGT CAATGCCCTC CATTCTCAAC TGAATCACTC CGAATGTACG 
GGGACTTACT TGCAGAACGG AGTGAGCGAC AGCCGAACAG GTGACAGCAT GGAAGCAAGT 
GGGTTTACTA AGACAGGGGA GCAAGAGAGG ACAGTGGGGA CTGTCCCGTC TCCTGGGACC 
CCCGCGGCTC CATGCTGTGG TCAGCTGGAG GAGCGCTGGC AGAAGCTGCA GAACCAAATG 
TTGGCTGAGC TAGACACGTG TAAGGAGAGT GCTCACGGGG TTCAGAGTGG GGTGTCTGCG 
ATCGAGGGCA GGGTGTTCCA GCTGGAGCAG ACATGCAGAA GGCTGGATAC CATCTCAGGG 
AGTCTGCAGA GGATCAAGGA GGGGCTCGGT AAGCATGTGG GCAGCTTGTG GAACTGCATC 
CGGCAGATGA ACGGGACGCT CAAATCGCAC TCCAGAGACA TCTCTGGCCT CAAGAATTCT 
GTGCAGCAAT TCTATAGCCA TGTTTTCCAG ATTTCTACCG ACTTGCAAGA TCTGGTCAAG 
TTTCAGCCAT CAGCAAAGGA ACCCTCAGAA GCAACCGAGG GACCGTCTGG GAAGACTCCC 
CTAGAATCCA CCAGACCTTC AGAGGAGGCC CCCACTGAAC CACCCCGGCT GACCCCACTG 
CCAGAAGACC CTGCAGGACC ACCACAGACA GGCCAGCAGC CAGTATTGCC CCAGAGGCCC 
CTGCAGCCAC CACCTTTGCC TGCCTGGCCT GGTCGCACGG GGTTGCCCTT CTTGCCTGGC 
TCTAGTGGCG TCATCATGGA GACTGGAGAG GCGGGGCCTC CAGGCAGGAT GGGCGTGTCC 
GGAAGGGGCC TGCCTCGGGG AGTGGATGGC CAGATGGGGC AGGGGCCCAT TCATAGCTCG 
GAAGGCTATG CAGGGGCACC AGGCTACCCC AAGTCACCTC CTGTAACCAC CCCAGGGGTG 
CCGCTGCCCA CTCTGGTGTC CTTTTCAGCC GGTCTTACCC AGAAGCCTTT TCCCAGCGAT 
GGAGGCGTTG TTCTCTTCAA TAAGGTGCTG GTGAACGATG GGGATGTATA CAACCCCAAC 
ACTGGGATCT TCACCGCACC ATACGACGGG CGCTACTTGA TCACAGCCAC CCTCACCCCT 
GAGAGAGACA CCTATGTGGA AGCCGTCCTG TCTGTCTCCA ACGCCAGTGT TGCCCAGCTG 
CACACGGCTG GGTACAGGAG AGAGTTCCTG GAATACCATC GTCCCCCTGG GGCTGTGCAT 
ACCTGTGGGG GCCCGGGGGC CTTCCACCTC ATTGTGCACT TGAAGGCAGG AGATGGAGTC 
AATGTTGTGG TGACTGGGGG CAGACTGGCT CACACAGACT TTGATGAAAT GTACTCCACC 
TTCAGTGGTG TTTTCTTGTA CCCTTTCCTT TCTCACCTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Emilin2 ( NM_001357338.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001344267.1 Mus musculus elastin microfibril interfacer 2 (Emilin2), transcript variant 4, mRNA.

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CloneID	OMu53595
Clone ID Related Accession (Same CDS sequence)	XM_006524286.3 , NM_001357338.1
Accession Version	NM_001357338.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3192bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	EMILIN-2 isoform 4 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY232991.1, AK155797.1, AK155909.1 and BY582438.1. On Oct 26, 2017 this sequence version replaced XM_006524286.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK155797.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164135 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGTCAGG AGACCCCGCC CCGACCCCGA GCACCCAGTC GCTGGACGCC GGCCCTGCTG 
GCCCTGCTGG CCCTGGGCGG CGCGGGACTG TGCCACGCGA GCTCGCAGCC TGGGTACCAC 
GCGAGGCCCA GTGCCAGGAA CAAAAACTGG TGCGCCTACA TCGTGAATAA GAACGTGAGC 
TGCACGGTAC AGGAGGGAAG CGAGAGTTTT ATTCAAGCTC AGTACAACTG TCCCTGGAAC 
CAGATGCCCT GTCCATCCGC CCTGGTGTAT CGGGTAAACT TTAGACCCAG ATTTGTCACT 
AGATACAAGA TAGTGACACA GTTGGAATGG AGATGCTGTC CTGGCTTTAG AGGACCAGAC 
TGCCAAGAAG GTCCCAAAGA CCACATGAAG ACCCCCCGGC CCCCATCAGC TCGACCAAAA 
AACAACCTGA AGAAAGCCAC AGATACAGAT CCAAGCCAAG TTTCTCAGCC TAAGAAAACG 
TTGTCCCCAA CAAATGCAGT GGAACCAGGT CAGGTTGCAG ATGCAAAGCA AGGCCCTCCG 
GAACTTCAGC AATCCAAAGT CCAGGTGCTG GAGGAGAAGG TCGTCCGACT CACCAGGATG 
GTGCTGGATC TCCAGTCCAC CGTGGTAGGC CTGAAGGAGA ACCTCAAACA CACCATTCAA 
GATGATGGCA GAAAAGAGCC AGACTCCTGG CTCGGTCCCC TGCACCCACA GCCAACGCCT 
GACAGCCCCC TTGCGGGGGA CGCAGAGCCG AGCCAGCTTC CCGGAATCCC CAGCAGCAAG 
GAATCTGGTA TGAAGGACAT CAAGTCTGAG TTGGCTGAGG TCAAAGACAC ACTGAAGACC 
AAGAGTGACA AGCTAGAAGA GCTGGACGGG AAGGTGAAGG GCTATGAAGG ACAGCTCAAG 
CAGCTGCAGG AGGCGGCTCA GGGTCCCACG GTCACCATGA CAACCAATGA GCTGTACCAA 
GCCTATGTGG ATAGCAAGAT CGATGCCCTG AGGGAGGAGC TCATGGAAGG CATGGACCGG 
AAGCTGGCTG ACCTGAAGAA TACGTGCGAG TACAAGCTGG TGGGGCTCCA GCAGCAGTGT 
GATGACTACG GCAGCAGCTA CCTCGGAGTG ATAGAGCTGA TCGGGGAGAA GGAGGCGAGC 
CTGAAAAAAG ACATTGCTGA CCTGCGAGCC CAGCTGCAGG ATCCTGTAGC CCAACCAAGC 
TGTTGCAATG GTCAAAAGAG CAGCGATTTC GGCCCACAGA TCAAGGCGCT AGACCAGAAA 
ATCGAGAGAG TTGCCGAGGC CACCAGGATG CTGAATGGAC GGCTGGACAA TGAGTTCGAC 
CGCCTCTCCG TTCCAGAGCC AGACGCAGAC TTTGATGCGA GATGGACCGA GCTCGACGCA 
AGGATCAATG TGACGGAGAA GAACGCAGAG GAGCATTGCT TTTACATTGA GGAGACCCTG 
CGGGGGACCA TCAACGGCGA AGTGGATGAC TTGAGGAAGC TTCTGAATGA GAAGATCCAC 
TCCCTGGAAG ACCGTCTGGG AATTGTTCTG CAAGCGGCTA ACAGCTCAGA CGTGGAGTTG 
ACACCCATGG GCCCAGCCTT GCCAGAGCAG CCAGGGGCAG AGAATGAGCA GGTCCTAATG 
GAGTTGAGCC GCCTGAAGGA CAAAGTTCAA GTGGTTGAGG ACTTCTGCCT GCAGAGCTTG 
CCCCACGGGA TAGATGGAGC TTTGCCTAGT GTAGAGGACC TCACGCATGT CAGCCTGAGC 
CTTTTAGAAT CCCTCAATGA TACGATGCAC AGGCAGTTCC AAGAAACGAG TCACAGCATC 
CAGAAACTCC AAGAGGACGT CAATGCCCTC CATTCTCAAC TGAATCACTC CGAATGTACG 
GGGACTTACT TGCAGAACGG AGTGAGCGAC AGCCGAACAG GTGACAGCAT GGAAGCAAGT 
GGGTTTACTA AGACAGGGGA GCAAGAGAGG ACAGTGGGGA CTGTCCCGTC TCCTGGGACC 
CCCGCGGCTC CATGCTGTGG TCAGCTGGAG GAGCGCTGGC AGAAGCTGCA GAACCAAATG 
TTGGCTGAGC TAGACACGTG TAAGGAGAGT GCTCACGGGG TTCAGAGTGG GGTGTCTGCG 
ATCGAGGGCA GGGTGTTCCA GCTGGAGCAG ACATGCAGAA GGCTGGATAC CATCTCAGGG 
AGTCTGCAGA GGATCAAGGA GGGGCTCGGT AAGCATGTGG GCAGCTTGTG GAACTGCATC 
CGGCAGATGA ACGGGACGCT CAAATCGCAC TCCAGAGACA TCTCTGGCCT CAAGAATTCT 
GTGCAGCAAT TCTATAGCCA TGTTTTCCAG ATTTCTACCG ACTTGCAAGA TCTGGTCAAG 
TTTCAGCCAT CAGCAACAGA GGAACCCTCA GAAGCAACCG AGGGACCGTC TGGGAAGACT 
CCCCTAGAAT CCACCAGACC TTCAGAGGAG GCCCCCACTG AACCACCCCG GCTGACCCCA 
CTGCCAGAAG ACCCTGCAGG ACCACCACAG ACAGGCCAGC AGCCAGTATT GCCCCAGAGG 
CCCCTGCAGC CACCACCTTT GCCTGCCTGG CCTGGTCGCA CGGGGTTGCC CTTCTTGCCT 
GGCTCTAGTG GCGTCATCAT GGAGACTGGA GAGGCGGGGC CTCCAGGCAG GATGGGCGTG 
TCCGGAAGGG GCCTGCCTCG GGGAGTGGAT GGCCAGATGG GGCAGGGGCC CATTCATAGC 
TCGGAAGGCT ATGCAGGGGC ACCAGGCTAC CCCAAGTCAC CTCCTGTAAC CACCCCAGCC 
GGTCTTACCC AGAAGCCTTT TCCCAGCGAT GGAGGCGTTG TTCTCTTCAA TAAGGTGCTG 
GTGAACGATG GGGATGTATA CAACCCCAAC ACTGGGATCT TCACCGCACC ATACGACGGG 
CGCTACTTGA TCACAGCCAC CCTCACCCCT GAGAGAGACA CCTATGTGGA AGCCGTCCTG 
TCTGTCTCCA ACGCCAGTGT TGCCCAGCTG CACACGGCTG GGTACAGGAG AGAGTTCCTG 
GAATACCATC GTCCCCCTGG GGCTGTGCAT ACCTGTGGGG GCCCGGGGGC CTTCCACCTC 
ATTGTGCACT TGAAGGCAGG AGATGGAGTC AATGTTGTGG TGACTGGGGG CAGACTGGCT 
CACACAGACT TTGATGAAAT GTACTCCACC TTCAGTGGTG TTTTCTTGTA CCCTTTCCTT 
TCTCACCTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001344267.1
CDS	152..3343
Translation	MCQETPPRPRAPSRWTPALLALLALGGAGLCHASSQPGYHARPSARNKNWCAYIVNKNVSCTVQEGSESFIQAQYNCPWNQMPCPSALVYRVNFRPRFVTRYKIVTQLEWRCCPGFRGPDCQEGPKDHMKTPRPPSARPKNNLKKATDTDPSQVSQPKKTLSPTNAVEPGQVADAKQGPPELQQSKVQVLEEKVVRLTRMVLDLQSTVVGLKENLKHTIQDDGRKEPDSWLGPLHPQPTPDSPLAGDAEPSQLPGIPSSKESGMKDIKSELAEVKDTLKTKSDKLEELDGKVKGYEGQLKQLQEAAQGPTVTMTTNELYQAYVDSKIDALREELMEGMDRKLADLKNTCEYKLVGLQQQCDDYGSSYLGVIELIGEKEASLKKDIADLRAQLQDPVAQPSCCNGQKSSDFGPQIKALDQKIERVAEATRMLNGRLDNEFDRLSVPEPDADFDARWTELDARINVTEKNAEEHCFYIEETLRGTINGEVDDLRKLLNEKIHSLEDRLGIVLQAANSSDVELTPMGPALPEQPGAENEQVLMELSRLKDKVQVVEDFCLQSLPHGIDGALPSVEDLTHVSLSLLESLNDTMHRQFQETSHSIQKLQEDVNALHSQLNHSECTGTYLQNGVSDSRTGDSMEASGFTKTGEQERTVGTVPSPGTPAAPCCGQLEERWQKLQNQMLAELDTCKESAHGVQSGVSAIEGRVFQLEQTCRRLDTISGSLQRIKEGLGKHVGSLWNCIRQMNGTLKSHSRDISGLKNSVQQFYSHVFQISTDLQDLVKFQPSATEEPSEATEGPSGKTPLESTRPSEEAPTEPPRLTPLPEDPAGPPQTGQQPVLPQRPLQPPPLPAWPGRTGLPFLPGSSGVIMETGEAGPPGRMGVSGRGLPRGVDGQMGQGPIHSSEGYAGAPGYPKSPPVTTPAGLTQKPFPSDGGVVLFNKVLVNDGDVYNPNTGIFTAPYDGRYLITATLTPERDTYVEAVLSVSNASVAQLHTAGYRREFLEYHRPPGAVHTCGGPGAFHLIVHLKAGDGVNVVVTGGRLAHTDFDEMYSTFSGVFLYPFLSHL

Target ORF information:

RefSeq Version	NM_001357338.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus elastin microfibril interfacer 2 (Emilin2), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001357338.1

ORF Insert Sequence:

ATGTGTCAGG AGACCCCGCC CCGACCCCGA GCACCCAGTC GCTGGACGCC GGCCCTGCTG 
GCCCTGCTGG CCCTGGGCGG CGCGGGACTG TGCCACGCGA GCTCGCAGCC TGGGTACCAC 
GCGAGGCCCA GTGCCAGGAA CAAAAACTGG TGCGCCTACA TCGTGAATAA GAACGTGAGC 
TGCACGGTAC AGGAGGGAAG CGAGAGTTTT ATTCAAGCTC AGTACAACTG TCCCTGGAAC 
CAGATGCCCT GTCCATCCGC CCTGGTGTAT CGGGTAAACT TTAGACCCAG ATTTGTCACT 
AGATACAAGA TAGTGACACA GTTGGAATGG AGATGCTGTC CTGGCTTTAG AGGACCAGAC 
TGCCAAGAAG GTCCCAAAGA CCACATGAAG ACCCCCCGGC CCCCATCAGC TCGACCAAAA 
AACAACCTGA AGAAAGCCAC AGATACAGAT CCAAGCCAAG TTTCTCAGCC TAAGAAAACG 
TTGTCCCCAA CAAATGCAGT GGAACCAGGT CAGGTTGCAG ATGCAAAGCA AGGCCCTCCG 
GAACTTCAGC AATCCAAAGT CCAGGTGCTG GAGGAGAAGG TCGTCCGACT CACCAGGATG 
GTGCTGGATC TCCAGTCCAC CGTGGTAGGC CTGAAGGAGA ACCTCAAACA CACCATTCAA 
GATGATGGCA GAAAAGAGCC AGACTCCTGG CTCGGTCCCC TGCACCCACA GCCAACGCCT 
GACAGCCCCC TTGCGGGGGA CGCAGAGCCG AGCCAGCTTC CCGGAATCCC CAGCAGCAAG 
GAATCTGGTA TGAAGGACAT CAAGTCTGAG TTGGCTGAGG TCAAAGACAC ACTGAAGACC 
AAGAGTGACA AGCTAGAAGA GCTGGACGGG AAGGTGAAGG GCTATGAAGG ACAGCTCAAG 
CAGCTGCAGG AGGCGGCTCA GGGTCCCACG GTCACCATGA CAACCAATGA GCTGTACCAA 
GCCTATGTGG ATAGCAAGAT CGATGCCCTG AGGGAGGAGC TCATGGAAGG CATGGACCGG 
AAGCTGGCTG ACCTGAAGAA TACGTGCGAG TACAAGCTGG TGGGGCTCCA GCAGCAGTGT 
GATGACTACG GCAGCAGCTA CCTCGGAGTG ATAGAGCTGA TCGGGGAGAA GGAGGCGAGC 
CTGAAAAAAG ACATTGCTGA CCTGCGAGCC CAGCTGCAGG ATCCTGTAGC CCAACCAAGC 
TGTTGCAATG GTCAAAAGAG CAGCGATTTC GGCCCACAGA TCAAGGCGCT AGACCAGAAA 
ATCGAGAGAG TTGCCGAGGC CACCAGGATG CTGAATGGAC GGCTGGACAA TGAGTTCGAC 
CGCCTCTCCG TTCCAGAGCC AGACGCAGAC TTTGATGCGA GATGGACCGA GCTCGACGCA 
AGGATCAATG TGACGGAGAA GAACGCAGAG GAGCATTGCT TTTACATTGA GGAGACCCTG 
CGGGGGACCA TCAACGGCGA AGTGGATGAC TTGAGGAAGC TTCTGAATGA GAAGATCCAC 
TCCCTGGAAG ACCGTCTGGG AATTGTTCTG CAAGCGGCTA ACAGCTCAGA CGTGGAGTTG 
ACACCCATGG GCCCAGCCTT GCCAGAGCAG CCAGGGGCAG AGAATGAGCA GGTCCTAATG 
GAGTTGAGCC GCCTGAAGGA CAAAGTTCAA GTGGTTGAGG ACTTCTGCCT GCAGAGCTTG 
CCCCACGGGA TAGATGGAGC TTTGCCTAGT GTAGAGGACC TCACGCATGT CAGCCTGAGC 
CTTTTAGAAT CCCTCAATGA TACGATGCAC AGGCAGTTCC AAGAAACGAG TCACAGCATC 
CAGAAACTCC AAGAGGACGT CAATGCCCTC CATTCTCAAC TGAATCACTC CGAATGTACG 
GGGACTTACT TGCAGAACGG AGTGAGCGAC AGCCGAACAG GTGACAGCAT GGAAGCAAGT 
GGGTTTACTA AGACAGGGGA GCAAGAGAGG ACAGTGGGGA CTGTCCCGTC TCCTGGGACC 
CCCGCGGCTC CATGCTGTGG TCAGCTGGAG GAGCGCTGGC AGAAGCTGCA GAACCAAATG 
TTGGCTGAGC TAGACACGTG TAAGGAGAGT GCTCACGGGG TTCAGAGTGG GGTGTCTGCG 
ATCGAGGGCA GGGTGTTCCA GCTGGAGCAG ACATGCAGAA GGCTGGATAC CATCTCAGGG 
AGTCTGCAGA GGATCAAGGA GGGGCTCGGT AAGCATGTGG GCAGCTTGTG GAACTGCATC 
CGGCAGATGA ACGGGACGCT CAAATCGCAC TCCAGAGACA TCTCTGGCCT CAAGAATTCT 
GTGCAGCAAT TCTATAGCCA TGTTTTCCAG ATTTCTACCG ACTTGCAAGA TCTGGTCAAG 
TTTCAGCCAT CAGCAACAGA GGAACCCTCA GAAGCAACCG AGGGACCGTC TGGGAAGACT 
CCCCTAGAAT CCACCAGACC TTCAGAGGAG GCCCCCACTG AACCACCCCG GCTGACCCCA 
CTGCCAGAAG ACCCTGCAGG ACCACCACAG ACAGGCCAGC AGCCAGTATT GCCCCAGAGG 
CCCCTGCAGC CACCACCTTT GCCTGCCTGG CCTGGTCGCA CGGGGTTGCC CTTCTTGCCT 
GGCTCTAGTG GCGTCATCAT GGAGACTGGA GAGGCGGGGC CTCCAGGCAG GATGGGCGTG 
TCCGGAAGGG GCCTGCCTCG GGGAGTGGAT GGCCAGATGG GGCAGGGGCC CATTCATAGC 
TCGGAAGGCT ATGCAGGGGC ACCAGGCTAC CCCAAGTCAC CTCCTGTAAC CACCCCAGCC 
GGTCTTACCC AGAAGCCTTT TCCCAGCGAT GGAGGCGTTG TTCTCTTCAA TAAGGTGCTG 
GTGAACGATG GGGATGTATA CAACCCCAAC ACTGGGATCT TCACCGCACC ATACGACGGG 
CGCTACTTGA TCACAGCCAC CCTCACCCCT GAGAGAGACA CCTATGTGGA AGCCGTCCTG 
TCTGTCTCCA ACGCCAGTGT TGCCCAGCTG CACACGGCTG GGTACAGGAG AGAGTTCCTG 
GAATACCATC GTCCCCCTGG GGCTGTGCAT ACCTGTGGGG GCCCGGGGGC CTTCCACCTC 
ATTGTGCACT TGAAGGCAGG AGATGGAGTC AATGTTGTGG TGACTGGGGG CAGACTGGCT 
CACACAGACT TTGATGAAAT GTACTCCACC TTCAGTGGTG TTTTCTTGTA CCCTTTCCTT 
TCTCACCTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Emilin2 ( XM_006524285.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524348.1 Mus musculus elastin microfibril interfacer 2 (Emilin2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu53594
Clone ID Related Accession (Same CDS sequence)	XM_006524285.3 , NM_001357337.1
Accession Version	XM_006524285.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	EMILIN-2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006524285.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTCAGG AGACCCCGCC CCGACCCCGA GCACCCAGTC GCTGGACGCC GGCCCTGCTG 
GCCCTGCTGG CCCTGGGCGG CGCGGGACTG TGCCACGCGA GCTCGCAGCC TGGGTACCAC 
GCGAGGCCCA GTGCCAGGAA CAAAAACTGG TGCGCCTACA TCGTGAATAA GAACGTGAGC 
TGCACGGTAC AGGAGGGAAG CGAGAGTTTT ATTCAAGCTC AGTACAACTG TCCCTGGAAC 
CAGATGCCCT GTCCATCCGC CCTGGTGTAT CGGGTAAACT TTAGACCCAG ATTTGTCACT 
AGATACAAGA TAGTGACACA GTTGGAATGG AGATGCTGTC CTGGCTTTAG AGGACCAGAC 
TGCCAAGAAG GTCCCAAAGA CCACATGAAG ACCCCCCGGC CCCCATCAGC TCGACCAAAA 
AACAACCTGA AGAAAGCCAC AGATACAGAT CCAAGCCAAG TTTCTCAGCC TAAGAAAACG 
TTGTCCCCAA CAAATGCAGT GGAACCAGGT CAGGTTGCAG ATGCAAAGCA AGGCCCTCCG 
GAACTTCAGC AATCCAAAGT CCAGGTGCTG GAGGAGAAGG TCGTCCGACT CACCAGGATG 
GTGCTGGATC TCCAGTCCAC CGTGGTAGGC CTGAAGGAGA ACCTCAAACA CACCATTCAA 
GATGATGGCA GAAAAGAGCC AGACTCCTGG CTCGGTCCCC TGCACCCACA GCCAACGCCT 
GACAGCCCCC TTGCGGGGGA CGCAGAGCCG AGCCAGCTTC CCGGAATCCC CAGCAGCAAG 
GAATCTGGTA TGAAGGACAT CAAGTCTGAG TTGGCTGAGG TCAAAGACAC ACTGAAGACC 
AAGAGTGACA AGCTAGAAGA GCTGGACGGG AAGGTGAAGG GCTATGAAGG ACAGCTCAAG 
CAGCTGCAGG AGGCGGCTCA GGGTCCCACG GTCACCATGA CAACCAATGA GCTGTACCAA 
GCCTATGTGG ATAGCAAGAT CGATGCCCTG AGGGAGGAGC TCATGGAAGG CATGGACCGG 
AAGCTGGCTG ACCTGAAGAA TACGTGCGAG TACAAGCTGG TGGGGCTCCA GCAGCAGTGT 
GATGACTACG GCAGCAGCTA CCTCGGAGTG ATAGAGCTGA TCGGGGAGAA GGAGGCGAGC 
CTGAAAAAAG ACATTGCTGA CCTGCGAGCC CAGCTGCAGG ATCCTGTAGC CCAACCAAGC 
TGTTGCAATG GTCAAAAGAG CAGCGATTTC GGCCCACAGA TCAAGGCGCT AGACCAGAAA 
ATCGAGAGAG TTGCCGAGGC CACCAGGATG CTGAATGGAC GGCTGGACAA TGAGTTCGAC 
CGCCTCTCCG TTCCAGAGCC AGACGCAGAC TTTGATGCGA GATGGACCGA GCTCGACGCA 
AGGATCAATG TGACGGAGAA GAACGCAGAG GAGCATTGCT TTTACATTGA GGAGACCCTG 
CGGGGGACCA TCAACGGCGA AGTGGATGAC TTGAGGAAGC TTCTGAATGA GAAGATCCAC 
TCCCTGGAAG ACCGTCTGGG AATTGTTCTG CAAGCGGCTA ACAGCTCAGA CGTGGAGTTG 
ACACCCATGG GCCCAGCCTT GCCAGAGCAG CCAGGGGCAG AGAATGAGCA GGTCCTAATG 
GAGTTGAGCC GCCTGAAGGA CAAAGTTCAA GTGGTTGAGG ACTTCTGCCT GCAGAGCTTG 
CCCCACGGGA TAGATGGAGC TTTGCCTAGT GTAGAGGACC TCACGCATGT CAGCCTGAGC 
CTTTTAGAAT CCCTCAATGA TACGATGCAC AGGCAGTTCC AAGAAACGAG TCACAGCATC 
CAGAAACTCC AAGAGGACGT CAATGCCCTC CATTCTCAAC TGAATCACTC CGAATGTACG 
GGGACTTACT TGCAGAACGG AGTGAGCGAC AGCCGAACAG GTGACAGCAT GGAAGCAAGT 
GGGTTTACTA AGACAGGGGA GCAAGAGAGG ACAGTGGGGA CTGTCCCGTC TCCTGGGACC 
CCCGCGGCTC CATGCTGTGG TCAGCTGGAG GAGCGCTGGC AGAAGCTGCA GAACCAAATG 
TTGGCTGAGC TAGACACGTG TAAGGAGAGT GCTCACGGGG TTCAGAGTGG GGTGTCTGCG 
ATCGAGGGCA GGGTGTTCCA GCTGGAGCAG ACATGCAGAA GGCTGGATAC CATCTCAGGG 
AGTCTGCAGA GGATCAAGGA GGGGCTCGGT AAGCATGTGG GCAGCTTGTG GAACTGCATC 
CGGCAGATGA ACGGGACGCT CAAATCGCAC TCCAGAGACA TCTCTGGCCT CAAGAATTCT 
GTGCAGCAAT TCTATAGCCA TGTTTTCCAG ATTTCTACCG ACTTGCAAGA TCTGGTCAAG 
TTTCAGCCAT CAGCAAAGGA ACCCTCAGAA GCAACCGAGG GACCGTCTGG GAAGACTCCC 
CTAGAATCCA CCAGACCTTC AGAGGAGGCC CCCACTGAAC CACCCCGGCT GACCCCACTG 
CCAGAAGACC CTGCAGGACC ACCACAGACA GGCCAGCAGC CAGTATTGCC CCAGAGGCCC 
CTGCAGCCAC CACCTTTGCC TGCCTGGCCT GGTCGCACGG GGTTGCCCTT CTTGCCTGGC 
TCTAGTGGCG TCATCATGGA GACTGGAGAG GCGGGGCCTC CAGGCAGGAT GGGCGTGTCC 
GGAAGGGGCC TGCCTCGGGG AGTGGATGGC CAGATGGGGC AGGGGCCCAT TCATAGCTCG 
GAAGGCTATG CAGGGGCACC AGGCTACCCC AAGTCACCTC CTGTAACCAC CCCAGGGGTG 
CCGCTGCCCA CTCTGGTGTC CTTTTCAGCC GGTCTTACCC AGAAGCCTTT TCCCAGCGAT 
GGAGGCGTTG TTCTCTTCAA TAAGGTGCTG GTGAACGATG GGGATGTATA CAACCCCAAC 
ACTGGGATCT TCACCGCACC ATACGACGGG CGCTACTTGA TCACAGCCAC CCTCACCCCT 
GAGAGAGACA CCTATGTGGA AGCCGTCCTG TCTGTCTCCA ACGCCAGTGT TGCCCAGCTG 
CACACGGCTG GGTACAGGAG AGAGTTCCTG GAATACCATC GTCCCCCTGG GGCTGTGCAT 
ACCTGTGGGG GCCCGGGGGC CTTCCACCTC ATTGTGCACT TGAAGGCAGG AGATGGAGTC 
AATGTTGTGG TGACTGGGGG CAGACTGGCT CACACAGACT TTGATGAAAT GTACTCCACC 
TTCAGTGGTG TTTTCTTGTA CCCTTTCCTT TCTCACCTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524348.1
CDS	459..3680
Translation	MCQETPPRPRAPSRWTPALLALLALGGAGLCHASSQPGYHARPSARNKNWCAYIVNKNVSCTVQEGSESFIQAQYNCPWNQMPCPSALVYRVNFRPRFVTRYKIVTQLEWRCCPGFRGPDCQEGPKDHMKTPRPPSARPKNNLKKATDTDPSQVSQPKKTLSPTNAVEPGQVADAKQGPPELQQSKVQVLEEKVVRLTRMVLDLQSTVVGLKENLKHTIQDDGRKEPDSWLGPLHPQPTPDSPLAGDAEPSQLPGIPSSKESGMKDIKSELAEVKDTLKTKSDKLEELDGKVKGYEGQLKQLQEAAQGPTVTMTTNELYQAYVDSKIDALREELMEGMDRKLADLKNTCEYKLVGLQQQCDDYGSSYLGVIELIGEKEASLKKDIADLRAQLQDPVAQPSCCNGQKSSDFGPQIKALDQKIERVAEATRMLNGRLDNEFDRLSVPEPDADFDARWTELDARINVTEKNAEEHCFYIEETLRGTINGEVDDLRKLLNEKIHSLEDRLGIVLQAANSSDVELTPMGPALPEQPGAENEQVLMELSRLKDKVQVVEDFCLQSLPHGIDGALPSVEDLTHVSLSLLESLNDTMHRQFQETSHSIQKLQEDVNALHSQLNHSECTGTYLQNGVSDSRTGDSMEASGFTKTGEQERTVGTVPSPGTPAAPCCGQLEERWQKLQNQMLAELDTCKESAHGVQSGVSAIEGRVFQLEQTCRRLDTISGSLQRIKEGLGKHVGSLWNCIRQMNGTLKSHSRDISGLKNSVQQFYSHVFQISTDLQDLVKFQPSAKEPSEATEGPSGKTPLESTRPSEEAPTEPPRLTPLPEDPAGPPQTGQQPVLPQRPLQPPPLPAWPGRTGLPFLPGSSGVIMETGEAGPPGRMGVSGRGLPRGVDGQMGQGPIHSSEGYAGAPGYPKSPPVTTPGVPLPTLVSFSAGLTQKPFPSDGGVVLFNKVLVNDGDVYNPNTGIFTAPYDGRYLITATLTPERDTYVEAVLSVSNASVAQLHTAGYRREFLEYHRPPGAVHTCGGPGAFHLIVHLKAGDGVNVVVTGGRLAHTDFDEMYSTFSGVFLYPFLSHL

Target ORF information:

RefSeq Version	XM_006524285.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus elastin microfibril interfacer 2 (Emilin2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524285.3

ORF Insert Sequence:

ATGTGTCAGG AGACCCCGCC CCGACCCCGA GCACCCAGTC GCTGGACGCC GGCCCTGCTG 
GCCCTGCTGG CCCTGGGCGG CGCGGGACTG TGCCACGCGA GCTCGCAGCC TGGGTACCAC 
GCGAGGCCCA GTGCCAGGAA CAAAAACTGG TGCGCCTACA TCGTGAATAA GAACGTGAGC 
TGCACGGTAC AGGAGGGAAG CGAGAGTTTT ATTCAAGCTC AGTACAACTG TCCCTGGAAC 
CAGATGCCCT GTCCATCCGC CCTGGTGTAT CGGGTAAACT TTAGACCCAG ATTTGTCACT 
AGATACAAGA TAGTGACACA GTTGGAATGG AGATGCTGTC CTGGCTTTAG AGGACCAGAC 
TGCCAAGAAG GTCCCAAAGA CCACATGAAG ACCCCCCGGC CCCCATCAGC TCGACCAAAA 
AACAACCTGA AGAAAGCCAC AGATACAGAT CCAAGCCAAG TTTCTCAGCC TAAGAAAACG 
TTGTCCCCAA CAAATGCAGT GGAACCAGGT CAGGTTGCAG ATGCAAAGCA AGGCCCTCCG 
GAACTTCAGC AATCCAAAGT CCAGGTGCTG GAGGAGAAGG TCGTCCGACT CACCAGGATG 
GTGCTGGATC TCCAGTCCAC CGTGGTAGGC CTGAAGGAGA ACCTCAAACA CACCATTCAA 
GATGATGGCA GAAAAGAGCC AGACTCCTGG CTCGGTCCCC TGCACCCACA GCCAACGCCT 
GACAGCCCCC TTGCGGGGGA CGCAGAGCCG AGCCAGCTTC CCGGAATCCC CAGCAGCAAG 
GAATCTGGTA TGAAGGACAT CAAGTCTGAG TTGGCTGAGG TCAAAGACAC ACTGAAGACC 
AAGAGTGACA AGCTAGAAGA GCTGGACGGG AAGGTGAAGG GCTATGAAGG ACAGCTCAAG 
CAGCTGCAGG AGGCGGCTCA GGGTCCCACG GTCACCATGA CAACCAATGA GCTGTACCAA 
GCCTATGTGG ATAGCAAGAT CGATGCCCTG AGGGAGGAGC TCATGGAAGG CATGGACCGG 
AAGCTGGCTG ACCTGAAGAA TACGTGCGAG TACAAGCTGG TGGGGCTCCA GCAGCAGTGT 
GATGACTACG GCAGCAGCTA CCTCGGAGTG ATAGAGCTGA TCGGGGAGAA GGAGGCGAGC 
CTGAAAAAAG ACATTGCTGA CCTGCGAGCC CAGCTGCAGG ATCCTGTAGC CCAACCAAGC 
TGTTGCAATG GTCAAAAGAG CAGCGATTTC GGCCCACAGA TCAAGGCGCT AGACCAGAAA 
ATCGAGAGAG TTGCCGAGGC CACCAGGATG CTGAATGGAC GGCTGGACAA TGAGTTCGAC 
CGCCTCTCCG TTCCAGAGCC AGACGCAGAC TTTGATGCGA GATGGACCGA GCTCGACGCA 
AGGATCAATG TGACGGAGAA GAACGCAGAG GAGCATTGCT TTTACATTGA GGAGACCCTG 
CGGGGGACCA TCAACGGCGA AGTGGATGAC TTGAGGAAGC TTCTGAATGA GAAGATCCAC 
TCCCTGGAAG ACCGTCTGGG AATTGTTCTG CAAGCGGCTA ACAGCTCAGA CGTGGAGTTG 
ACACCCATGG GCCCAGCCTT GCCAGAGCAG CCAGGGGCAG AGAATGAGCA GGTCCTAATG 
GAGTTGAGCC GCCTGAAGGA CAAAGTTCAA GTGGTTGAGG ACTTCTGCCT GCAGAGCTTG 
CCCCACGGGA TAGATGGAGC TTTGCCTAGT GTAGAGGACC TCACGCATGT CAGCCTGAGC 
CTTTTAGAAT CCCTCAATGA TACGATGCAC AGGCAGTTCC AAGAAACGAG TCACAGCATC 
CAGAAACTCC AAGAGGACGT CAATGCCCTC CATTCTCAAC TGAATCACTC CGAATGTACG 
GGGACTTACT TGCAGAACGG AGTGAGCGAC AGCCGAACAG GTGACAGCAT GGAAGCAAGT 
GGGTTTACTA AGACAGGGGA GCAAGAGAGG ACAGTGGGGA CTGTCCCGTC TCCTGGGACC 
CCCGCGGCTC CATGCTGTGG TCAGCTGGAG GAGCGCTGGC AGAAGCTGCA GAACCAAATG 
TTGGCTGAGC TAGACACGTG TAAGGAGAGT GCTCACGGGG TTCAGAGTGG GGTGTCTGCG 
ATCGAGGGCA GGGTGTTCCA GCTGGAGCAG ACATGCAGAA GGCTGGATAC CATCTCAGGG 
AGTCTGCAGA GGATCAAGGA GGGGCTCGGT AAGCATGTGG GCAGCTTGTG GAACTGCATC 
CGGCAGATGA ACGGGACGCT CAAATCGCAC TCCAGAGACA TCTCTGGCCT CAAGAATTCT 
GTGCAGCAAT TCTATAGCCA TGTTTTCCAG ATTTCTACCG ACTTGCAAGA TCTGGTCAAG 
TTTCAGCCAT CAGCAAAGGA ACCCTCAGAA GCAACCGAGG GACCGTCTGG GAAGACTCCC 
CTAGAATCCA CCAGACCTTC AGAGGAGGCC CCCACTGAAC CACCCCGGCT GACCCCACTG 
CCAGAAGACC CTGCAGGACC ACCACAGACA GGCCAGCAGC CAGTATTGCC CCAGAGGCCC 
CTGCAGCCAC CACCTTTGCC TGCCTGGCCT GGTCGCACGG GGTTGCCCTT CTTGCCTGGC 
TCTAGTGGCG TCATCATGGA GACTGGAGAG GCGGGGCCTC CAGGCAGGAT GGGCGTGTCC 
GGAAGGGGCC TGCCTCGGGG AGTGGATGGC CAGATGGGGC AGGGGCCCAT TCATAGCTCG 
GAAGGCTATG CAGGGGCACC AGGCTACCCC AAGTCACCTC CTGTAACCAC CCCAGGGGTG 
CCGCTGCCCA CTCTGGTGTC CTTTTCAGCC GGTCTTACCC AGAAGCCTTT TCCCAGCGAT 
GGAGGCGTTG TTCTCTTCAA TAAGGTGCTG GTGAACGATG GGGATGTATA CAACCCCAAC 
ACTGGGATCT TCACCGCACC ATACGACGGG CGCTACTTGA TCACAGCCAC CCTCACCCCT 
GAGAGAGACA CCTATGTGGA AGCCGTCCTG TCTGTCTCCA ACGCCAGTGT TGCCCAGCTG 
CACACGGCTG GGTACAGGAG AGAGTTCCTG GAATACCATC GTCCCCCTGG GGCTGTGCAT 
ACCTGTGGGG GCCCGGGGGC CTTCCACCTC ATTGTGCACT TGAAGGCAGG AGATGGAGTC 
AATGTTGTGG TGACTGGGGG CAGACTGGCT CACACAGACT TTGATGAAAT GTACTCCACC 
TTCAGTGGTG TTTTCTTGTA CCCTTTCCTT TCTCACCTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Emilin2 ( XM_006524286.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524349.1 Mus musculus elastin microfibril interfacer 2 (Emilin2), transcript variant X2, mRNA.

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CloneID	OMu53595
Clone ID Related Accession (Same CDS sequence)	XM_006524286.3 , NM_001357338.1
Accession Version	XM_006524286.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3192bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	EMILIN-2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006524286.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTCAGG AGACCCCGCC CCGACCCCGA GCACCCAGTC GCTGGACGCC GGCCCTGCTG 
GCCCTGCTGG CCCTGGGCGG CGCGGGACTG TGCCACGCGA GCTCGCAGCC TGGGTACCAC 
GCGAGGCCCA GTGCCAGGAA CAAAAACTGG TGCGCCTACA TCGTGAATAA GAACGTGAGC 
TGCACGGTAC AGGAGGGAAG CGAGAGTTTT ATTCAAGCTC AGTACAACTG TCCCTGGAAC 
CAGATGCCCT GTCCATCCGC CCTGGTGTAT CGGGTAAACT TTAGACCCAG ATTTGTCACT 
AGATACAAGA TAGTGACACA GTTGGAATGG AGATGCTGTC CTGGCTTTAG AGGACCAGAC 
TGCCAAGAAG GTCCCAAAGA CCACATGAAG ACCCCCCGGC CCCCATCAGC TCGACCAAAA 
AACAACCTGA AGAAAGCCAC AGATACAGAT CCAAGCCAAG TTTCTCAGCC TAAGAAAACG 
TTGTCCCCAA CAAATGCAGT GGAACCAGGT CAGGTTGCAG ATGCAAAGCA AGGCCCTCCG 
GAACTTCAGC AATCCAAAGT CCAGGTGCTG GAGGAGAAGG TCGTCCGACT CACCAGGATG 
GTGCTGGATC TCCAGTCCAC CGTGGTAGGC CTGAAGGAGA ACCTCAAACA CACCATTCAA 
GATGATGGCA GAAAAGAGCC AGACTCCTGG CTCGGTCCCC TGCACCCACA GCCAACGCCT 
GACAGCCCCC TTGCGGGGGA CGCAGAGCCG AGCCAGCTTC CCGGAATCCC CAGCAGCAAG 
GAATCTGGTA TGAAGGACAT CAAGTCTGAG TTGGCTGAGG TCAAAGACAC ACTGAAGACC 
AAGAGTGACA AGCTAGAAGA GCTGGACGGG AAGGTGAAGG GCTATGAAGG ACAGCTCAAG 
CAGCTGCAGG AGGCGGCTCA GGGTCCCACG GTCACCATGA CAACCAATGA GCTGTACCAA 
GCCTATGTGG ATAGCAAGAT CGATGCCCTG AGGGAGGAGC TCATGGAAGG CATGGACCGG 
AAGCTGGCTG ACCTGAAGAA TACGTGCGAG TACAAGCTGG TGGGGCTCCA GCAGCAGTGT 
GATGACTACG GCAGCAGCTA CCTCGGAGTG ATAGAGCTGA TCGGGGAGAA GGAGGCGAGC 
CTGAAAAAAG ACATTGCTGA CCTGCGAGCC CAGCTGCAGG ATCCTGTAGC CCAACCAAGC 
TGTTGCAATG GTCAAAAGAG CAGCGATTTC GGCCCACAGA TCAAGGCGCT AGACCAGAAA 
ATCGAGAGAG TTGCCGAGGC CACCAGGATG CTGAATGGAC GGCTGGACAA TGAGTTCGAC 
CGCCTCTCCG TTCCAGAGCC AGACGCAGAC TTTGATGCGA GATGGACCGA GCTCGACGCA 
AGGATCAATG TGACGGAGAA GAACGCAGAG GAGCATTGCT TTTACATTGA GGAGACCCTG 
CGGGGGACCA TCAACGGCGA AGTGGATGAC TTGAGGAAGC TTCTGAATGA GAAGATCCAC 
TCCCTGGAAG ACCGTCTGGG AATTGTTCTG CAAGCGGCTA ACAGCTCAGA CGTGGAGTTG 
ACACCCATGG GCCCAGCCTT GCCAGAGCAG CCAGGGGCAG AGAATGAGCA GGTCCTAATG 
GAGTTGAGCC GCCTGAAGGA CAAAGTTCAA GTGGTTGAGG ACTTCTGCCT GCAGAGCTTG 
CCCCACGGGA TAGATGGAGC TTTGCCTAGT GTAGAGGACC TCACGCATGT CAGCCTGAGC 
CTTTTAGAAT CCCTCAATGA TACGATGCAC AGGCAGTTCC AAGAAACGAG TCACAGCATC 
CAGAAACTCC AAGAGGACGT CAATGCCCTC CATTCTCAAC TGAATCACTC CGAATGTACG 
GGGACTTACT TGCAGAACGG AGTGAGCGAC AGCCGAACAG GTGACAGCAT GGAAGCAAGT 
GGGTTTACTA AGACAGGGGA GCAAGAGAGG ACAGTGGGGA CTGTCCCGTC TCCTGGGACC 
CCCGCGGCTC CATGCTGTGG TCAGCTGGAG GAGCGCTGGC AGAAGCTGCA GAACCAAATG 
TTGGCTGAGC TAGACACGTG TAAGGAGAGT GCTCACGGGG TTCAGAGTGG GGTGTCTGCG 
ATCGAGGGCA GGGTGTTCCA GCTGGAGCAG ACATGCAGAA GGCTGGATAC CATCTCAGGG 
AGTCTGCAGA GGATCAAGGA GGGGCTCGGT AAGCATGTGG GCAGCTTGTG GAACTGCATC 
CGGCAGATGA ACGGGACGCT CAAATCGCAC TCCAGAGACA TCTCTGGCCT CAAGAATTCT 
GTGCAGCAAT TCTATAGCCA TGTTTTCCAG ATTTCTACCG ACTTGCAAGA TCTGGTCAAG 
TTTCAGCCAT CAGCAACAGA GGAACCCTCA GAAGCAACCG AGGGACCGTC TGGGAAGACT 
CCCCTAGAAT CCACCAGACC TTCAGAGGAG GCCCCCACTG AACCACCCCG GCTGACCCCA 
CTGCCAGAAG ACCCTGCAGG ACCACCACAG ACAGGCCAGC AGCCAGTATT GCCCCAGAGG 
CCCCTGCAGC CACCACCTTT GCCTGCCTGG CCTGGTCGCA CGGGGTTGCC CTTCTTGCCT 
GGCTCTAGTG GCGTCATCAT GGAGACTGGA GAGGCGGGGC CTCCAGGCAG GATGGGCGTG 
TCCGGAAGGG GCCTGCCTCG GGGAGTGGAT GGCCAGATGG GGCAGGGGCC CATTCATAGC 
TCGGAAGGCT ATGCAGGGGC ACCAGGCTAC CCCAAGTCAC CTCCTGTAAC CACCCCAGCC 
GGTCTTACCC AGAAGCCTTT TCCCAGCGAT GGAGGCGTTG TTCTCTTCAA TAAGGTGCTG 
GTGAACGATG GGGATGTATA CAACCCCAAC ACTGGGATCT TCACCGCACC ATACGACGGG 
CGCTACTTGA TCACAGCCAC CCTCACCCCT GAGAGAGACA CCTATGTGGA AGCCGTCCTG 
TCTGTCTCCA ACGCCAGTGT TGCCCAGCTG CACACGGCTG GGTACAGGAG AGAGTTCCTG 
GAATACCATC GTCCCCCTGG GGCTGTGCAT ACCTGTGGGG GCCCGGGGGC CTTCCACCTC 
ATTGTGCACT TGAAGGCAGG AGATGGAGTC AATGTTGTGG TGACTGGGGG CAGACTGGCT 
CACACAGACT TTGATGAAAT GTACTCCACC TTCAGTGGTG TTTTCTTGTA CCCTTTCCTT 
TCTCACCTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524349.1
CDS	460..3651
Translation	MCQETPPRPRAPSRWTPALLALLALGGAGLCHASSQPGYHARPSARNKNWCAYIVNKNVSCTVQEGSESFIQAQYNCPWNQMPCPSALVYRVNFRPRFVTRYKIVTQLEWRCCPGFRGPDCQEGPKDHMKTPRPPSARPKNNLKKATDTDPSQVSQPKKTLSPTNAVEPGQVADAKQGPPELQQSKVQVLEEKVVRLTRMVLDLQSTVVGLKENLKHTIQDDGRKEPDSWLGPLHPQPTPDSPLAGDAEPSQLPGIPSSKESGMKDIKSELAEVKDTLKTKSDKLEELDGKVKGYEGQLKQLQEAAQGPTVTMTTNELYQAYVDSKIDALREELMEGMDRKLADLKNTCEYKLVGLQQQCDDYGSSYLGVIELIGEKEASLKKDIADLRAQLQDPVAQPSCCNGQKSSDFGPQIKALDQKIERVAEATRMLNGRLDNEFDRLSVPEPDADFDARWTELDARINVTEKNAEEHCFYIEETLRGTINGEVDDLRKLLNEKIHSLEDRLGIVLQAANSSDVELTPMGPALPEQPGAENEQVLMELSRLKDKVQVVEDFCLQSLPHGIDGALPSVEDLTHVSLSLLESLNDTMHRQFQETSHSIQKLQEDVNALHSQLNHSECTGTYLQNGVSDSRTGDSMEASGFTKTGEQERTVGTVPSPGTPAAPCCGQLEERWQKLQNQMLAELDTCKESAHGVQSGVSAIEGRVFQLEQTCRRLDTISGSLQRIKEGLGKHVGSLWNCIRQMNGTLKSHSRDISGLKNSVQQFYSHVFQISTDLQDLVKFQPSATEEPSEATEGPSGKTPLESTRPSEEAPTEPPRLTPLPEDPAGPPQTGQQPVLPQRPLQPPPLPAWPGRTGLPFLPGSSGVIMETGEAGPPGRMGVSGRGLPRGVDGQMGQGPIHSSEGYAGAPGYPKSPPVTTPAGLTQKPFPSDGGVVLFNKVLVNDGDVYNPNTGIFTAPYDGRYLITATLTPERDTYVEAVLSVSNASVAQLHTAGYRREFLEYHRPPGAVHTCGGPGAFHLIVHLKAGDGVNVVVTGGRLAHTDFDEMYSTFSGVFLYPFLSHL

Target ORF information:

RefSeq Version	XM_006524286.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus elastin microfibril interfacer 2 (Emilin2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524286.3

ORF Insert Sequence:

ATGTGTCAGG AGACCCCGCC CCGACCCCGA GCACCCAGTC GCTGGACGCC GGCCCTGCTG 
GCCCTGCTGG CCCTGGGCGG CGCGGGACTG TGCCACGCGA GCTCGCAGCC TGGGTACCAC 
GCGAGGCCCA GTGCCAGGAA CAAAAACTGG TGCGCCTACA TCGTGAATAA GAACGTGAGC 
TGCACGGTAC AGGAGGGAAG CGAGAGTTTT ATTCAAGCTC AGTACAACTG TCCCTGGAAC 
CAGATGCCCT GTCCATCCGC CCTGGTGTAT CGGGTAAACT TTAGACCCAG ATTTGTCACT 
AGATACAAGA TAGTGACACA GTTGGAATGG AGATGCTGTC CTGGCTTTAG AGGACCAGAC 
TGCCAAGAAG GTCCCAAAGA CCACATGAAG ACCCCCCGGC CCCCATCAGC TCGACCAAAA 
AACAACCTGA AGAAAGCCAC AGATACAGAT CCAAGCCAAG TTTCTCAGCC TAAGAAAACG 
TTGTCCCCAA CAAATGCAGT GGAACCAGGT CAGGTTGCAG ATGCAAAGCA AGGCCCTCCG 
GAACTTCAGC AATCCAAAGT CCAGGTGCTG GAGGAGAAGG TCGTCCGACT CACCAGGATG 
GTGCTGGATC TCCAGTCCAC CGTGGTAGGC CTGAAGGAGA ACCTCAAACA CACCATTCAA 
GATGATGGCA GAAAAGAGCC AGACTCCTGG CTCGGTCCCC TGCACCCACA GCCAACGCCT 
GACAGCCCCC TTGCGGGGGA CGCAGAGCCG AGCCAGCTTC CCGGAATCCC CAGCAGCAAG 
GAATCTGGTA TGAAGGACAT CAAGTCTGAG TTGGCTGAGG TCAAAGACAC ACTGAAGACC 
AAGAGTGACA AGCTAGAAGA GCTGGACGGG AAGGTGAAGG GCTATGAAGG ACAGCTCAAG 
CAGCTGCAGG AGGCGGCTCA GGGTCCCACG GTCACCATGA CAACCAATGA GCTGTACCAA 
GCCTATGTGG ATAGCAAGAT CGATGCCCTG AGGGAGGAGC TCATGGAAGG CATGGACCGG 
AAGCTGGCTG ACCTGAAGAA TACGTGCGAG TACAAGCTGG TGGGGCTCCA GCAGCAGTGT 
GATGACTACG GCAGCAGCTA CCTCGGAGTG ATAGAGCTGA TCGGGGAGAA GGAGGCGAGC 
CTGAAAAAAG ACATTGCTGA CCTGCGAGCC CAGCTGCAGG ATCCTGTAGC CCAACCAAGC 
TGTTGCAATG GTCAAAAGAG CAGCGATTTC GGCCCACAGA TCAAGGCGCT AGACCAGAAA 
ATCGAGAGAG TTGCCGAGGC CACCAGGATG CTGAATGGAC GGCTGGACAA TGAGTTCGAC 
CGCCTCTCCG TTCCAGAGCC AGACGCAGAC TTTGATGCGA GATGGACCGA GCTCGACGCA 
AGGATCAATG TGACGGAGAA GAACGCAGAG GAGCATTGCT TTTACATTGA GGAGACCCTG 
CGGGGGACCA TCAACGGCGA AGTGGATGAC TTGAGGAAGC TTCTGAATGA GAAGATCCAC 
TCCCTGGAAG ACCGTCTGGG AATTGTTCTG CAAGCGGCTA ACAGCTCAGA CGTGGAGTTG 
ACACCCATGG GCCCAGCCTT GCCAGAGCAG CCAGGGGCAG AGAATGAGCA GGTCCTAATG 
GAGTTGAGCC GCCTGAAGGA CAAAGTTCAA GTGGTTGAGG ACTTCTGCCT GCAGAGCTTG 
CCCCACGGGA TAGATGGAGC TTTGCCTAGT GTAGAGGACC TCACGCATGT CAGCCTGAGC 
CTTTTAGAAT CCCTCAATGA TACGATGCAC AGGCAGTTCC AAGAAACGAG TCACAGCATC 
CAGAAACTCC AAGAGGACGT CAATGCCCTC CATTCTCAAC TGAATCACTC CGAATGTACG 
GGGACTTACT TGCAGAACGG AGTGAGCGAC AGCCGAACAG GTGACAGCAT GGAAGCAAGT 
GGGTTTACTA AGACAGGGGA GCAAGAGAGG ACAGTGGGGA CTGTCCCGTC TCCTGGGACC 
CCCGCGGCTC CATGCTGTGG TCAGCTGGAG GAGCGCTGGC AGAAGCTGCA GAACCAAATG 
TTGGCTGAGC TAGACACGTG TAAGGAGAGT GCTCACGGGG TTCAGAGTGG GGTGTCTGCG 
ATCGAGGGCA GGGTGTTCCA GCTGGAGCAG ACATGCAGAA GGCTGGATAC CATCTCAGGG 
AGTCTGCAGA GGATCAAGGA GGGGCTCGGT AAGCATGTGG GCAGCTTGTG GAACTGCATC 
CGGCAGATGA ACGGGACGCT CAAATCGCAC TCCAGAGACA TCTCTGGCCT CAAGAATTCT 
GTGCAGCAAT TCTATAGCCA TGTTTTCCAG ATTTCTACCG ACTTGCAAGA TCTGGTCAAG 
TTTCAGCCAT CAGCAACAGA GGAACCCTCA GAAGCAACCG AGGGACCGTC TGGGAAGACT 
CCCCTAGAAT CCACCAGACC TTCAGAGGAG GCCCCCACTG AACCACCCCG GCTGACCCCA 
CTGCCAGAAG ACCCTGCAGG ACCACCACAG ACAGGCCAGC AGCCAGTATT GCCCCAGAGG 
CCCCTGCAGC CACCACCTTT GCCTGCCTGG CCTGGTCGCA CGGGGTTGCC CTTCTTGCCT 
GGCTCTAGTG GCGTCATCAT GGAGACTGGA GAGGCGGGGC CTCCAGGCAG GATGGGCGTG 
TCCGGAAGGG GCCTGCCTCG GGGAGTGGAT GGCCAGATGG GGCAGGGGCC CATTCATAGC 
TCGGAAGGCT ATGCAGGGGC ACCAGGCTAC CCCAAGTCAC CTCCTGTAAC CACCCCAGCC 
GGTCTTACCC AGAAGCCTTT TCCCAGCGAT GGAGGCGTTG TTCTCTTCAA TAAGGTGCTG 
GTGAACGATG GGGATGTATA CAACCCCAAC ACTGGGATCT TCACCGCACC ATACGACGGG 
CGCTACTTGA TCACAGCCAC CCTCACCCCT GAGAGAGACA CCTATGTGGA AGCCGTCCTG 
TCTGTCTCCA ACGCCAGTGT TGCCCAGCTG CACACGGCTG GGTACAGGAG AGAGTTCCTG 
GAATACCATC GTCCCCCTGG GGCTGTGCAT ACCTGTGGGG GCCCGGGGGC CTTCCACCTC 
ATTGTGCACT TGAAGGCAGG AGATGGAGTC AATGTTGTGG TGACTGGGGG CAGACTGGCT 
CACACAGACT TTGATGAAAT GTACTCCACC TTCAGTGGTG TTTTCTTGTA CCCTTTCCTT 
TCTCACCTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Altered feto-placental vascularization, feto-placental malperfusion and fetal growth restriction in mice with
Development (Cambridge, England)144(13)2469-2479(2017 07)
Lacko LA,Hurtado R,Hinds S,Poulos MG,Butler JM,Stuhlmann H

EMILIN2 regulates platelet activation, thrombus formation, and clot retraction.
PloS one10(2)e0115284(2015)
Huang M,Sannaningaiah D,Zhao N,Gong Y,Grondolsky J,Hoover-Plow J

Multiple-interactions among EMILIN1 and EMILIN2 N- and C-terminal domains.
Matrix biology : journal of the International Society for Matrix Biology4144-55(2015 Jan)
Bot S,Andreuzzi E,Capuano A,Schiavinato A,Colombatti A,Doliana R

Extracellular matrix secretion by cardiac fibroblasts: role of microRNA-29b and microRNA-30c.
Circulation research113(10)1138-47(2013 Oct)
Abonnenc M,Nabeebaccus AA,Mayr U,Barallobre-Barreiro J,Dong X,Cuello F,Sur S,Drozdov I,Langley SR,Lu R,Stathopoulou K,Didangelos A,Yin X,Zimmermann WH,Shah AM,Zampetaki A,Mayr M

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Emilin2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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