gRNAs
The following Zfp277 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Zfp277 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OMu03704 | |
| Clone ID Related Accession (Same CDS sequence) | NM_172575.3 | |
| Accession Version | NM_172575.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1752bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-05-25 | |
| Organism | Mus musculus(house mouse) | |
| Product | zinc finger protein 277 isoform 1 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY206876.1, AK077401.1 and AC123855.4. On May 28, 2009 this sequence version replaced NM_172575.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: AK077401.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGGCTGCTA GTGAGGTCCA GGGGCTTCCT GTGGACCCCC GGGAGGCTGG GGTCCGCGGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_766163.2 |
| CDS | 32..1783 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_172575.3 |
1 | ATGGCTGCTA GTGAGGTCCA GGGGCTTCCT GTGGACCCCC GGGAGGCTGG GGTCCGCGGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu07806 | |
| Clone ID Related Accession (Same CDS sequence) | NM_178845.3 | |
| Accession Version | NM_178845.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1374bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-05-25 | |
| Organism | Mus musculus(house mouse) | |
| Product | zinc finger protein 277 isoform 2 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY206876.1, AK075945.1 and AC123855.4. On May 28, 2009 this sequence version replaced NM_178845.2. Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting protein (isoform 2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660823.104338.1, SRR1660825.104338.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGGCTGCTA GTGAGGTCCA GGGGCTTCCT GTGGACCCCC GGGAGGCTGG GGTCCGCGGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_849173.2 |
| CDS | 32..1405 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_178845.3 |
1 | ATGGCTGCTA GTGAGGTCCA GGGGCTTCCT GTGGACCCCC GGGAGGCTGG GGTCCGCGGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Divergent effects of muscarinic receptor subtype gene ablation on murine colon tumorigenesis reveals association of M3R and zinc finger protein 277 expression in colon neoplasia. |
A novel zinc finger protein Zfp277 mediates transcriptional repression of the Ink4a/arf locus through polycomb repressive complex 1. |
Resolution of cell fate decisions revealed by single-cell gene expression analysis from zygote to blastocyst. |
A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58. |
Tagging genes with cassette-exchange sites. |
