Layn cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Layn
Entrez Gene ID	244864
Full Name	layilin
Synonyms	E030012M19Rik,Gm511
General protein information	Preferred Names layilin Names layilin
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9\|9 A5.3

mRNA and Protein(s)

mRNA	Protein	Name
XM_006510329.3	XP_006510392.1	layilin isoform X1
XM_006510330.4	XP_006510393.1	layilin isoform X2
NM_001033534.1	NP_001028706.1	layilin precursor
XM_006510330.3	XP_006510393.1	layilin isoform X2

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Layn	NP_001178926.1
Homo sapiens (human)	LAYN	NP_001245319.1
Xenopus tropicalis (tropical clawed frog)	layn	XP_002932981.2
Pan troglodytes (chimpanzee)	LAYN	XP_508749.2
	LAYN	XP_001105695.1
Bos taurus (cattle)	LAYN	XP_005215935.1
Canis lupus familiaris (dog)	LAYN	XP_005619853.1
Mus musculus (house mouse)	Layn	NP_001028706.1
Danio rerio (zebrafish)	layna	XP_693396.4

Gene Ontology
Bibliography

Related articles in PubMed

Layilin is critical for mediating hyaluronan 35kDa-induced intestinal epithelial tight junction protein ZO-1 in vitro and in vivo.
Kim Y, West GA, Ray G, Kessler SP, Petrey AC, Fiocchi C, McDonald C, Longworth MS, Nagy LE, de la Motte CA
Matrix biology : journal of the International Society for Matrix Biology(2017 Oct)

Roles of layilin in TNF-?-induced epithelial-mesenchymal transformation of renal tubular epithelial cells.
Adachi T, Arito M, Suematsu N, Kamijo-Ikemori A, Omoteyama K, Sato T, Kurokawa MS, Okamoto K, Kimura K, Shibagaki Y, Kato T
Biochemical and biophysical research communications467(1)63-9(2015 Nov)

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Thompson CL, Ng L, Menon V, Martinez S, Lee CK, Glattfelder K, Sunkin SM, Henry A, Lau C, Dang C, Garcia-Lopez R, Martinez-Ferre A, Pombero A, Rubenstein JLR, Wakeman WB, Hohmann J, Dee N, Sodt AJ, Young R, Smith K, Nguyen TN, Kidney J, Kuan L, Jeromin A, Kaykas A, Miller J, Page D, Orta G, Bernard A, Riley Z, Smith S, Wohnoutka P, Hawrylycz MJ, Puelles L, Jones AR
Neuron83(2)309-323(2014 Jul)

A mouse knockout library for secreted and transmembrane proteins.
Tang T, Li L, Tang J, Li Y, Lin WY, Martin F, Grant D, Solloway M, Parker L, Ye W, Forrest W, Ghilardi N, Oravecz T, Platt KA, Rice DS, Hansen GM, Abuin A, Eberhart DE, Godowski P, Holt KH, Peterson A, Zambrowicz BP, de Sauvage FJ
Nature biotechnology28(7)749-55(2010 Jul)

Structural basis for the interaction between the cytoplasmic domain of the hyaluronate receptor layilin and the talin F3 subdomain.
Wegener KL, Basran J, Bagshaw CR, Campbell ID, Roberts GC, Critchley DR, Barsukov IL
Journal of molecular biology382(1)112-26(2008 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

LAYN is involved in the TNF-alpha-induced epithelial-mesenchymal transformation (EMT) of renal tubular epithelial cells.
Title: Roles of layilin in TNF-?-induced epithelial-mesenchymal transformation of renal tubular epithelial cells.

The structure of the talin/layilin complex, which shows that talin binds integrins, PIPK1gamma and layilin in similar although subtly different ways, is reported.
Title: Structural basis for the interaction between the cytoplasmic domain of the hyaluronate receptor layilin and the talin F3 subdomain.

The following Layn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Layn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu12717	NM_001033534.1 Latest version!	Mus musculus layilin (Layn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu42211	XM_006510329.3 Latest version!	Mus musculus layilin (Layn), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu42212	XM_006510330.4 Latest version!	Mus musculus layilin (Layn), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OMu42212	XM_006510330.3	Mus musculus layilin (Layn), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Layn ( NM_001033534.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001028706.1 Mus musculus layilin (Layn), mRNA.

Price & Availability↑

CloneID	OMu12717
Clone ID Related Accession (Same CDS sequence)	NM_001033534.1
Accession Version	NM_001033534.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Mus musculus(house mouse)
Product	layilin precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC119831.6. On or before Apr 19, 2009 this sequence version replaced XM_917279.3, XM_146887.7. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGCAGCCGG GAGCAGCGCT GCAGGCTATG TTGCTGGCGG TGCTGCTGGC CAAACCGAGG 
GATTCGAAGG GTCGCCTGCT GAGCGCCTCG GACTTGGACC CCAGAGGAGG GCAGCTGGTC 
TGCCGAGGAG GAACACGGAG GCCTTGCTAT AAAGTCATTT ATTTCCATGA CGCCTTTCAA 
AGACTGAACT TTGAGGAAGC CAAAGAAACC TGCATGGAGG ATGGGGGACA GCTCGTCAGC 
ATTGAGACAG AGGATGAGCA GAGGTTGATA GAAAAGTTCA TTGAAAACCT CCTGGCATCT 
GATGGTGACT TCTGGATTGG CCTCAAGAGG CTGGAGGAGA AACAGAGCAA TAACACAGCC 
TGCCAGGACC TTTATGCTTG GACAGATGGA AGCACATCAC AGTTTAGGAA CTGGTATGTG 
GATGAGCCTT CCTGTGGCAG TGAGGTCTGC GTGGTCATGT ACCATCAGCC ATCAGCACCA 
CCCGGCATCG GGGGCTCGTA CATGTTCCAG TGGAATGATG ACCGGTGCAA CATGAAGAAC 
AATTTCATTT GCAAATATCA TGACGATAAA CCAAGTACAA CACCCTCTAT TTGGCCTGGA 
GGTGAAGCAA CTGAGCCAGC AACACCCTTA CTTCCAGAAG AAACACAGAA AGAAGACACC 
AAAGAAACAT TTAAAGAACG AAGAGAGGCT GCTTTGAATC TTGCCTACAT CCTAATTCCC 
AGTATTCCCC TTTTCCTCCT ACTTGTGGTC ACTTCAGCTG TATGTTGGGT TTGGATCTGT 
AGAAGAAAAC GAGAACAGAC AGACCCTAGC ACAAAGGAGC AGCACACCAT TTGGCCCACT 
CCTCGCCAAG AAAACAGCCC CAACCTAGAT GTTTACAATG TCATCAGAAA ACAAAGTGAA 
GCTGATTTAG CTGAGCCCCG GCCAGACCTG AAGAACATCT CCTTCCGGGT GTGTTCTGGG 
GAAGCTATGC CTGATGACAT GTCTTGTGAC TATGAAAACA TTGCTGTGAA CCCTTCGGAA 
AGTGGGTTTG TGACTTTGGC AAGTATGGAG AGTGGATTTG TGACCAATGA CATCTATGAA 
TTCTCCCCGG ACCGAATGGG GAGGAGCAAG GAGAGTGGAT GGGTAGAAAA TGAAATATAT 
TACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001028706.1
CDS	125..1270
Translation	MQPGAALQAMLLAVLLAKPRDSKGRLLSASDLDPRGGQLVCRGGTRRPCYKVIYFHDAFQRLNFEEAKETCMEDGGQLVSIETEDEQRLIEKFIENLLASDGDFWIGLKRLEEKQSNNTACQDLYAWTDGSTSQFRNWYVDEPSCGSEVCVVMYHQPSAPPGIGGSYMFQWNDDRCNMKNNFICKYHDDKPSTTPSIWPGGEATEPATPLLPEETQKEDTKETFKERREAALNLAYILIPSIPLFLLLVVTSAVCWVWICRRKREQTDPSTKEQHTIWPTPRQENSPNLDVYNVIRKQSEADLAEPRPDLKNISFRVCSGEAMPDDMSCDYENIAVNPSESGFVTLASMESGFVTNDIYEFSPDRMGRSKESGWVENEIYY

Target ORF information:

RefSeq Version	NM_001033534.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus layilin (Layn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001033534.1

ORF Insert Sequence:

ATGCAGCCGG GAGCAGCGCT GCAGGCTATG TTGCTGGCGG TGCTGCTGGC CAAACCGAGG 
GATTCGAAGG GTCGCCTGCT GAGCGCCTCG GACTTGGACC CCAGAGGAGG GCAGCTGGTC 
TGCCGAGGAG GAACACGGAG GCCTTGCTAT AAAGTCATTT ATTTCCATGA CGCCTTTCAA 
AGACTGAACT TTGAGGAAGC CAAAGAAACC TGCATGGAGG ATGGGGGACA GCTCGTCAGC 
ATTGAGACAG AGGATGAGCA GAGGTTGATA GAAAAGTTCA TTGAAAACCT CCTGGCATCT 
GATGGTGACT TCTGGATTGG CCTCAAGAGG CTGGAGGAGA AACAGAGCAA TAACACAGCC 
TGCCAGGACC TTTATGCTTG GACAGATGGA AGCACATCAC AGTTTAGGAA CTGGTATGTG 
GATGAGCCTT CCTGTGGCAG TGAGGTCTGC GTGGTCATGT ACCATCAGCC ATCAGCACCA 
CCCGGCATCG GGGGCTCGTA CATGTTCCAG TGGAATGATG ACCGGTGCAA CATGAAGAAC 
AATTTCATTT GCAAATATCA TGACGATAAA CCAAGTACAA CACCCTCTAT TTGGCCTGGA 
GGTGAAGCAA CTGAGCCAGC AACACCCTTA CTTCCAGAAG AAACACAGAA AGAAGACACC 
AAAGAAACAT TTAAAGAACG AAGAGAGGCT GCTTTGAATC TTGCCTACAT CCTAATTCCC 
AGTATTCCCC TTTTCCTCCT ACTTGTGGTC ACTTCAGCTG TATGTTGGGT TTGGATCTGT 
AGAAGAAAAC GAGAACAGAC AGACCCTAGC ACAAAGGAGC AGCACACCAT TTGGCCCACT 
CCTCGCCAAG AAAACAGCCC CAACCTAGAT GTTTACAATG TCATCAGAAA ACAAAGTGAA 
GCTGATTTAG CTGAGCCCCG GCCAGACCTG AAGAACATCT CCTTCCGGGT GTGTTCTGGG 
GAAGCTATGC CTGATGACAT GTCTTGTGAC TATGAAAACA TTGCTGTGAA CCCTTCGGAA 
AGTGGGTTTG TGACTTTGGC AAGTATGGAG AGTGGATTTG TGACCAATGA CATCTATGAA 
TTCTCCCCGG ACCGAATGGG GAGGAGCAAG GAGAGTGGAT GGGTAGAAAA TGAAATATAT 
TACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Layn ( XM_006510329.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006510392.1 Mus musculus layilin (Layn), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu42211
Clone ID Related Accession (Same CDS sequence)	XM_006510329.3
Accession Version	XM_006510329.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1122bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	layilin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006510329.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCCGG GAGCAGCGCT GCAGGCTATG TTGCTGGCGG TGCTGCTGGC CAAACCGAGG 
GATTCGAAGG GTCGCCTGCT GAGCGGGCAG CTGGTCTGCC GAGGAGGAAC ACGGAGGCCT 
TGCTATAAAG TCATTTATTT CCATGACGCC TTTCAAAGAC TGAACTTTGA GGAAGCCAAA 
GAAACCTGCA TGGAGGATGG GGGACAGCTC GTCAGCATTG AGACAGAGGA TGAGCAGAGG 
TTGATAGAAA AGTTCATTGA AAACCTCCTG GCATCTGATG GTGACTTCTG GATTGGCCTC 
AAGAGGCTGG AGGAGAAACA GAGCAATAAC ACAGCCTGCC AGGACCTTTA TGCTTGGACA 
GATGGAAGCA CATCACAGTT TAGGAACTGG TATGTGGATG AGCCTTCCTG TGGCAGTGAG 
GTCTGCGTGG TCATGTACCA TCAGCCATCA GCACCACCCG GCATCGGGGG CTCGTACATG 
TTCCAGTGGA ATGATGACCG GTGCAACATG AAGAACAATT TCATTTGCAA ATATCATGAC 
GATAAACCAA GTACAACACC CTCTATTTGG CCTGGAGGTG AAGCAACTGA GCCAGCAACA 
CCCTTACTTC CAGAAGAAAC ACAGAAAGAA GACACCAAAG AAACATTTAA AGAACGAAGA 
GAGGCTGCTT TGAATCTTGC CTACATCCTA ATTCCCAGTA TTCCCCTTTT CCTCCTACTT 
GTGGTCACTT CAGCTGTATG TTGGGTTTGG ATCTGTAGAA GAAAACGAGA ACAGACAGAC 
CCTAGCACAA AGGAGCAGCA CACCATTTGG CCCACTCCTC GCCAAGAAAA CAGCCCCAAC 
CTAGATGTTT ACAATGTCAT CAGAAAACAA AGTGAAGCTG ATTTAGCTGA GCCCCGGCCA 
GACCTGAAGA ACATCTCCTT CCGGGTGTGT TCTGGGGAAG CTATGCCTGA TGACATGTCT 
TGTGACTATG AAAACATTGC TGTGAACCCT TCGGAAAGTG GGTTTGTGAC TTTGGCAAGT 
ATGGAGAGTG GATTTGTGAC CAATGACATC TATGAATTCT CCCCGGACCG AATGGGGAGG 
AGCAAGGAGA GTGGATGGGT AGAAAATGAA ATATATTACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006510392.1
CDS	240..1361
Translation	MQPGAALQAMLLAVLLAKPRDSKGRLLSGQLVCRGGTRRPCYKVIYFHDAFQRLNFEEAKETCMEDGGQLVSIETEDEQRLIEKFIENLLASDGDFWIGLKRLEEKQSNNTACQDLYAWTDGSTSQFRNWYVDEPSCGSEVCVVMYHQPSAPPGIGGSYMFQWNDDRCNMKNNFICKYHDDKPSTTPSIWPGGEATEPATPLLPEETQKEDTKETFKERREAALNLAYILIPSIPLFLLLVVTSAVCWVWICRRKREQTDPSTKEQHTIWPTPRQENSPNLDVYNVIRKQSEADLAEPRPDLKNISFRVCSGEAMPDDMSCDYENIAVNPSESGFVTLASMESGFVTNDIYEFSPDRMGRSKESGWVENEIYY

Target ORF information:

RefSeq Version	XM_006510329.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus layilin (Layn), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006510329.3

ORF Insert Sequence:

ATGCAGCCGG GAGCAGCGCT GCAGGCTATG TTGCTGGCGG TGCTGCTGGC CAAACCGAGG 
GATTCGAAGG GTCGCCTGCT GAGCGGGCAG CTGGTCTGCC GAGGAGGAAC ACGGAGGCCT 
TGCTATAAAG TCATTTATTT CCATGACGCC TTTCAAAGAC TGAACTTTGA GGAAGCCAAA 
GAAACCTGCA TGGAGGATGG GGGACAGCTC GTCAGCATTG AGACAGAGGA TGAGCAGAGG 
TTGATAGAAA AGTTCATTGA AAACCTCCTG GCATCTGATG GTGACTTCTG GATTGGCCTC 
AAGAGGCTGG AGGAGAAACA GAGCAATAAC ACAGCCTGCC AGGACCTTTA TGCTTGGACA 
GATGGAAGCA CATCACAGTT TAGGAACTGG TATGTGGATG AGCCTTCCTG TGGCAGTGAG 
GTCTGCGTGG TCATGTACCA TCAGCCATCA GCACCACCCG GCATCGGGGG CTCGTACATG 
TTCCAGTGGA ATGATGACCG GTGCAACATG AAGAACAATT TCATTTGCAA ATATCATGAC 
GATAAACCAA GTACAACACC CTCTATTTGG CCTGGAGGTG AAGCAACTGA GCCAGCAACA 
CCCTTACTTC CAGAAGAAAC ACAGAAAGAA GACACCAAAG AAACATTTAA AGAACGAAGA 
GAGGCTGCTT TGAATCTTGC CTACATCCTA ATTCCCAGTA TTCCCCTTTT CCTCCTACTT 
GTGGTCACTT CAGCTGTATG TTGGGTTTGG ATCTGTAGAA GAAAACGAGA ACAGACAGAC 
CCTAGCACAA AGGAGCAGCA CACCATTTGG CCCACTCCTC GCCAAGAAAA CAGCCCCAAC 
CTAGATGTTT ACAATGTCAT CAGAAAACAA AGTGAAGCTG ATTTAGCTGA GCCCCGGCCA 
GACCTGAAGA ACATCTCCTT CCGGGTGTGT TCTGGGGAAG CTATGCCTGA TGACATGTCT 
TGTGACTATG AAAACATTGC TGTGAACCCT TCGGAAAGTG GGTTTGTGAC TTTGGCAAGT 
ATGGAGAGTG GATTTGTGAC CAATGACATC TATGAATTCT CCCCGGACCG AATGGGGAGG 
AGCAAGGAGA GTGGATGGGT AGAAAATGAA ATATATTACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Layn ( XM_006510330.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006510393.1 Mus musculus layilin (Layn), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu42212
Clone ID Related Accession (Same CDS sequence)	XM_006510330.4 , XM_006510330.3
Accession Version	XM_006510330.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	layilin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006510330.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCCGG GAGCAGCGCT GCAGGCTATG TTGCTGGCGG TGCTGCTGGC CAAACCGAGG 
GATTCGAAGG GTCGCCTGCT GAGCGCCTCG GACTTGGACC CCAGAGGAGG GCAGCTGGTC 
TGCCGAGGAG GAACACGGAG GCCTTGCTAT AAAGTCATTT ATTTCCATGA CGCCTTTCAA 
AGACTGAACT TTGAGGAAGC CAAAGAAACC TGCATGGAGG ATGGGGGACA GCTCGTCAGC 
ATTGAGACAG AGGATGAGCA GAGGTTGATA GAAAAGTTCA TTGAAAACCT CCTGGCATCT 
GATGGTGACT TCTGGATTGG CCTCAAGAGG CTGGAGGAGA AACAGAGCAA TAACACAGCC 
TGCCAGGACC TTTATGCTTG GACAGATGGA AGCACATCAC AGTTTAGGAA CTGGTATGTG 
GATGAGCCTT CCTGTGGCAG TGAGGTCTGC GTGGTCATGT ACCATCAGCC ATCAGCACCA 
CCCGGCATCG GGGGCTCGTA CATGTTCCAG TGGAATGATG ACCGGTGCAA CATGAAGAAC 
AATTTCATTT GCAAATATCA TGACGATAAA CCAAGTACAA CACCCTCTAT TTGGCCTGGA 
GGTGAAGCAA CTGAGCCAGC AACACCCTTA CTTCCAGAAG AAACACAGAA AGAAGACACC 
AAAGAAACAT TTAAAGAACG AAGAGAAAAC GAGAACAGAC AGACCCTAGC ACAAAGGAGC 
AGCACACCAT TTGGCCCACT CCTCGCCAAG AAAACAGCCC CAACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006510393.1
CDS	240..1007
Translation	MQPGAALQAMLLAVLLAKPRDSKGRLLSASDLDPRGGQLVCRGGTRRPCYKVIYFHDAFQRLNFEEAKETCMEDGGQLVSIETEDEQRLIEKFIENLLASDGDFWIGLKRLEEKQSNNTACQDLYAWTDGSTSQFRNWYVDEPSCGSEVCVVMYHQPSAPPGIGGSYMFQWNDDRCNMKNNFICKYHDDKPSTTPSIWPGGEATEPATPLLPEETQKEDTKETFKERRENENRQTLAQRSSTPFGPLLAKKTAPT

Target ORF information:

RefSeq Version	XM_006510330.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus layilin (Layn), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006510330.3

ORF Insert Sequence:

ATGCAGCCGG GAGCAGCGCT GCAGGCTATG TTGCTGGCGG TGCTGCTGGC CAAACCGAGG 
GATTCGAAGG GTCGCCTGCT GAGCGCCTCG GACTTGGACC CCAGAGGAGG GCAGCTGGTC 
TGCCGAGGAG GAACACGGAG GCCTTGCTAT AAAGTCATTT ATTTCCATGA CGCCTTTCAA 
AGACTGAACT TTGAGGAAGC CAAAGAAACC TGCATGGAGG ATGGGGGACA GCTCGTCAGC 
ATTGAGACAG AGGATGAGCA GAGGTTGATA GAAAAGTTCA TTGAAAACCT CCTGGCATCT 
GATGGTGACT TCTGGATTGG CCTCAAGAGG CTGGAGGAGA AACAGAGCAA TAACACAGCC 
TGCCAGGACC TTTATGCTTG GACAGATGGA AGCACATCAC AGTTTAGGAA CTGGTATGTG 
GATGAGCCTT CCTGTGGCAG TGAGGTCTGC GTGGTCATGT ACCATCAGCC ATCAGCACCA 
CCCGGCATCG GGGGCTCGTA CATGTTCCAG TGGAATGATG ACCGGTGCAA CATGAAGAAC 
AATTTCATTT GCAAATATCA TGACGATAAA CCAAGTACAA CACCCTCTAT TTGGCCTGGA 
GGTGAAGCAA CTGAGCCAGC AACACCCTTA CTTCCAGAAG AAACACAGAA AGAAGACACC 
AAAGAAACAT TTAAAGAACG AAGAGAAAAC GAGAACAGAC AGACCCTAGC ACAAAGGAGC 
AGCACACCAT TTGGCCCACT CCTCGCCAAG AAAACAGCCC CAACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Layn ( XM_006510330.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006510393.1 Mus musculus layilin (Layn), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu42212
Clone ID Related Accession (Same CDS sequence)	XM_006510330.4 , XM_006510330.3
Accession Version	XM_006510330.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	layilin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006510330.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCCGG GAGCAGCGCT GCAGGCTATG TTGCTGGCGG TGCTGCTGGC CAAACCGAGG 
GATTCGAAGG GTCGCCTGCT GAGCGCCTCG GACTTGGACC CCAGAGGAGG GCAGCTGGTC 
TGCCGAGGAG GAACACGGAG GCCTTGCTAT AAAGTCATTT ATTTCCATGA CGCCTTTCAA 
AGACTGAACT TTGAGGAAGC CAAAGAAACC TGCATGGAGG ATGGGGGACA GCTCGTCAGC 
ATTGAGACAG AGGATGAGCA GAGGTTGATA GAAAAGTTCA TTGAAAACCT CCTGGCATCT 
GATGGTGACT TCTGGATTGG CCTCAAGAGG CTGGAGGAGA AACAGAGCAA TAACACAGCC 
TGCCAGGACC TTTATGCTTG GACAGATGGA AGCACATCAC AGTTTAGGAA CTGGTATGTG 
GATGAGCCTT CCTGTGGCAG TGAGGTCTGC GTGGTCATGT ACCATCAGCC ATCAGCACCA 
CCCGGCATCG GGGGCTCGTA CATGTTCCAG TGGAATGATG ACCGGTGCAA CATGAAGAAC 
AATTTCATTT GCAAATATCA TGACGATAAA CCAAGTACAA CACCCTCTAT TTGGCCTGGA 
GGTGAAGCAA CTGAGCCAGC AACACCCTTA CTTCCAGAAG AAACACAGAA AGAAGACACC 
AAAGAAACAT TTAAAGAACG AAGAGAAAAC GAGAACAGAC AGACCCTAGC ACAAAGGAGC 
AGCACACCAT TTGGCCCACT CCTCGCCAAG AAAACAGCCC CAACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006510393.1
CDS	240..1007
Translation	MQPGAALQAMLLAVLLAKPRDSKGRLLSASDLDPRGGQLVCRGGTRRPCYKVIYFHDAFQRLNFEEAKETCMEDGGQLVSIETEDEQRLIEKFIENLLASDGDFWIGLKRLEEKQSNNTACQDLYAWTDGSTSQFRNWYVDEPSCGSEVCVVMYHQPSAPPGIGGSYMFQWNDDRCNMKNNFICKYHDDKPSTTPSIWPGGEATEPATPLLPEETQKEDTKETFKERRENENRQTLAQRSSTPFGPLLAKKTAPT

Target ORF information:

RefSeq Version	XM_006510330.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus layilin (Layn), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006510330.4

ORF Insert Sequence:

ATGCAGCCGG GAGCAGCGCT GCAGGCTATG TTGCTGGCGG TGCTGCTGGC CAAACCGAGG 
GATTCGAAGG GTCGCCTGCT GAGCGCCTCG GACTTGGACC CCAGAGGAGG GCAGCTGGTC 
TGCCGAGGAG GAACACGGAG GCCTTGCTAT AAAGTCATTT ATTTCCATGA CGCCTTTCAA 
AGACTGAACT TTGAGGAAGC CAAAGAAACC TGCATGGAGG ATGGGGGACA GCTCGTCAGC 
ATTGAGACAG AGGATGAGCA GAGGTTGATA GAAAAGTTCA TTGAAAACCT CCTGGCATCT 
GATGGTGACT TCTGGATTGG CCTCAAGAGG CTGGAGGAGA AACAGAGCAA TAACACAGCC 
TGCCAGGACC TTTATGCTTG GACAGATGGA AGCACATCAC AGTTTAGGAA CTGGTATGTG 
GATGAGCCTT CCTGTGGCAG TGAGGTCTGC GTGGTCATGT ACCATCAGCC ATCAGCACCA 
CCCGGCATCG GGGGCTCGTA CATGTTCCAG TGGAATGATG ACCGGTGCAA CATGAAGAAC 
AATTTCATTT GCAAATATCA TGACGATAAA CCAAGTACAA CACCCTCTAT TTGGCCTGGA 
GGTGAAGCAA CTGAGCCAGC AACACCCTTA CTTCCAGAAG AAACACAGAA AGAAGACACC 
AAAGAAACAT TTAAAGAACG AAGAGAAAAC GAGAACAGAC AGACCCTAGC ACAAAGGAGC 
AGCACACCAT TTGGCCCACT CCTCGCCAAG AAAACAGCCC CAACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Layilin is critical for mediating hyaluronan 35kDa-induced intestinal epithelial tight junction protein ZO-1 in vitro and in vivo.
Matrix biology : journal of the International Society for Matrix Biology(2017 Oct)
Kim Y,West GA,Ray G,Kessler SP,Petrey AC,Fiocchi C,McDonald C,Longworth MS,Nagy LE,de la Motte CA

Roles of layilin in TNF-?-induced epithelial-mesenchymal transformation of renal tubular epithelial cells.
Biochemical and biophysical research communications467(1)63-9(2015 Nov)
Adachi T,Arito M,Suematsu N,Kamijo-Ikemori A,Omoteyama K,Sato T,Kurokawa MS,Okamoto K,Kimura K,Shibagaki Y,Kato T

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Neuron83(2)309-323(2014 Jul)
Thompson CL,Ng L,Menon V,Martinez S,Lee CK,Glattfelder K,Sunkin SM,Henry A,Lau C,Dang C,Garcia-Lopez R,Martinez-Ferre A,Pombero A,Rubenstein JLR,Wakeman WB,Hohmann J,Dee N,Sodt AJ,Young R,Smith K,Nguyen TN,Kidney J,Kuan L,Jeromin A,Kaykas A,Miller J,Page D,Orta G,Bernard A,Riley Z,Smith S,Wohnoutka P,Hawrylycz MJ,Puelles L,Jones AR

A mouse knockout library for secreted and transmembrane proteins.
Nature biotechnology28(7)749-55(2010 Jul)
Tang T,Li L,Tang J,Li Y,Lin WY,Martin F,Grant D,Solloway M,Parker L,Ye W,Forrest W,Ghilardi N,Oravecz T,Platt KA,Rice DS,Hansen GM,Abuin A,Eberhart DE,Godowski P,Holt KH,Peterson A,Zambrowicz BP,de Sauvage FJ

Structural basis for the interaction between the cytoplasmic domain of the hyaluronate receptor layilin and the talin F3 subdomain.
Journal of molecular biology382(1)112-26(2008 Sep)
Wegener KL,Basran J,Bagshaw CR,Campbell ID,Roberts GC,Critchley DR,Barsukov IL

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Layn cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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