Itga8 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Itga8
Entrez Gene ID	241226
Full Name	integrin alpha 8
Synonyms	AI447669
General protein information	Preferred Names integrin alpha 8 Names integrin alpha-8
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2 A1\|2 9.12 cM
Summary	This gene encodes a member of the integrin family of cell surface proteins that mediate cellular interactions with the extracellular matrix and other cells. The encoded protein undergoes proteolytic processing to generate the disulfide-linked heterodimeric alpha subunit which, in turn associates with a beta subunit to form the functional integrin receptor. Mice lacking the encoded protein mostly die after birth due to kidney defects, but some of animals that survive exhibit defects in the sensory hair cells of the inner ear. [provided by RefSeq, Aug 2016].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006497475.3	XP_006497538.1	integrin alpha-8 isoform X1
NM_001001309.2	NP_001001309.1	integrin alpha-8 precursor
NM_001001309.3	NP_001001309.1	integrin alpha-8 preproprotein
XM_006497475.4	XP_006497538.1	integrin alpha-8 isoform X1
XM_006497476.3	XP_006497539.1	integrin alpha-8 isoform X2

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	ITGA8	NP_990619.1
Caenorhabditis elegans (roundworm)	pat-2	NP_498948.1
Pan troglodytes (chimpanzee)	ITGA8	XP_507671.2
Bos taurus (cattle)	ITGA8	XP_002692127.1
	ITGA8	XP_001089317.1
Drosophila melanogaster (fruit fly)	if	NP_001162778.1
Canis lupus familiaris (dog)	ITGA8	XP_005617255.1
Mus musculus (house mouse)	Itga8	NP_001001309.1
Homo sapiens (human)	ITGA8	NP_003629.1
Rattus norvegicus (Norway rat)	Itga8	NP_001167443.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000032	XP_311131.5
Xenopus tropicalis (tropical clawed frog)	itga8	XP_002939074.2

Gene Ontology
Bibliography

Related articles in PubMed

Sex differences in the development of vascular and renal lesions in mice with a simultaneous deficiency of
Marek I, Canu M, Cordasic N, Rauh M, Volkert G, Fahlbusch FB, Rascher W, Hilgers KF, Hartner A, Menendez-Castro C
Biology of sex differences819(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mechanistically, ligation of alpha8beta1 by the milk protein Mfge8 reduces antral smooth muscle contractile force by preventing RhoA activation through a PTEN-dependent mechanism.
Title: ?8?1 integrin regulates nutrient absorption through an Mfge8-PTEN dependent mechanism.

Itga8 seems to attenuate tubulointerstitial fibrosis in unilateral ureteral obstruction not via regulation of cell turnover, but via regulation of TGF-beta signalling, fibroblast activation and/or immune cell infiltration
Title: Alpha8 Integrin (Itga8) Signalling Attenuates Chronic Renal Interstitial Fibrosis by Reducing Fibroblast Activation, Not by Interfering with Regulation of Cell Turnover.

Fibrillin-1 and alpha8 integrin interact, and thus, regulate mesangial cell adhesion and migration.
Title: Fibrillin-1 and alpha8 integrin are co-expressed in the glomerulus and interact to convey adhesion of mesangial cells.

The findings indicate that QBRICK facilitates the integrin alpha8beta1-dependent interactions of cells with basement membranes by regulating the basement membrane assembly of nephronectin and explain why renal defects occur in Fraser syndrome.
Title: Basement membrane assembly of the integrin ?8?1 ligand nephronectin requires Fraser syndrome-associated proteins.

Alpha 8 integrin expression is strongly induced in a mouse model of renal fibrosis.
Title: Tubulointerstitial de novo expression of the ?8 integrin chain in a rodent model of renal fibrosis--a potential target for anti-fibrotic therapy?

The following Itga8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Itga8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu29155	XM_006497476.3 Latest version!	Mus musculus integrin alpha 8 (Itga8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu20099	NM_001001309.3 Latest version!	Mus musculus integrin alpha 8 (Itga8), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$629.30 $899.00
OMu29154	XM_006497475.4 Latest version!	Mus musculus integrin alpha 8 (Itga8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OMu20099	NM_001001309.2	Mus musculus integrin alpha 8 (Itga8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$629.30 $899.00
OMu29154	XM_006497475.3	Mus musculus integrin alpha 8 (Itga8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Itga8 ( XM_006497476.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497539.1 Mus musculus integrin alpha 8 (Itga8), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu29155
Clone ID Related Accession (Same CDS sequence)	XM_006497476.3
Accession Version	XM_006497476.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1863bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	integrin alpha-8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006497476.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAACA TCTTTGTAGA TTTGCTTGTA GGGGCATTTG GAAAAGGGAA AGTAGCTGTT 
TACAGAGCAA GGCCAGTTGT AACCGTGGAT GCCCAGCTTC TGCTGCACCC GATGATTATC 
AACCTTGAAA ATAAAACTTG CCAGATTCCA GAATTCCCTA CTCCTGTGGC CTGCTTTTCT 
GTAAGAGTCT GTGCATCTAT AGCAGGCCAG AGCATTTCAA ATACAATAGC CCTGCTGGCC 
GAGGTGCAGT TAGATTTCCT GAAGCAAAAA GGAGCCATCA AACGGACGCT CTTTCTCCAC 
AACCACCAGT CCCATTTCAC CTTCCCCTTT GTGATGAAGC AGCAGAAATC CCTCCACTGC 
CAGGATTTTA TGGTTTACCT TCGGGATGAA ACTGAATTCC GAGATAAATT GTCTCCAATC 
AACATCAGCC TGAACTACAG TTTGGATGAT TCTACCTTTA AAGACAGCCT GGAAGTGAAG 
CCAATTTTGA ACCACTACAG GGACAATGTA GTTACTGAGC AGGCTCACAT CCTGGTGGAC 
TGTGGAGAAG ACAATCTATG CGTTCCTGAC TTGAAGCTGT CAGCTAGACC AGATAAGCAT 
CAGATAATTA TTGGCGATGA AAATCACCTA ATGCTCATAA TAAATGCAAG AAATGAGGGA 
GAAGGGGCCT ACGAAGCCGA ACTCTTTGTT ATCATCCCCG AAGAAGCAGA TTATGTTGGG 
ATTGAGCGCA ACAACAAGGG ACTGAGGCCC CTGAGTTGTG AGTACAAGAT GGAAAACGTG 
ACCAGGATGG TGGTGTGTGA CCTTGGGAAC CCGATGGTGA CTGGAACAAA TTTTTCTCTG 
GGCCTCCGAT TTGCTGTTCC TCGCCTTGAG AAAACAAATA TGAGCATTAA CTTCGATCTC 
CAAATCAGAA GCTCCAATAA GGACAATCCT GACAGCAACT TCGAGCGTGT ACAGATCAAC 
ATCACTGCCA TTGCTCAGGT CGAAATCAGA GGAGTGTCCC ACCCTCCGCA GATTGTTCTG 
CCCATCCACA ACTGGGAACC AGAAAAGAAG CCACACAAAG AGGAGGAAGT CGGTCCTTTG 
GTTGAGCATA TTTATGAGCT GCACAATATT GGACCCAGCA CCATCAGTGA CAGCATTCTT 
GACGTGGGCT GGCCGTTCTC TGCACGGGAT GAGTTTCTCC TCTATATTTT TCATCTTCAA 
ACTCTGGGAC CTCTACAATG TCAAACAAAT CCTGAGATCA ACCCACAGGA TATAAAGCCT 
GCTGCCTCCC CAGAGGATAC CCCTGAGCTC AGTGCCTTCT TAAGAAATGC TACAATTCCC 
CATCTTGTCA GAAAGAGGGA TGTGCCAGTG GTCCAGCTCC ACAGACAGAG CCCTGCAAGA 
ATACTGAACT GTACCAACAT TGACTGCTTG CAAATCTCTT GTGCAGTGGG TCGCCTGGGA 
GGAGGCGAAA GTGCAGTCCT AAAGGTCAGA TCGAGATTGT GGGCCCACAC GTTCCTCAAG 
GAAATTTCTG TAGAAGAGAC GAGCCAAGAG CAGTTGGTGA AGACACTCAA GAGAAAGAAT 
GATCATTATG CTCTTGCATC CCTGGTGTCC TTTGAAGTCA AGAAGATGCC GTATAAAGAA 
CAGCCAGCAA AGCTGCCAGC GGGGAGCACA GCGGTTAAGA CGTCGGTTAT TTGGGCCACT 
CCGAATGTCT CCTTCTCAAT TCCATTGTGG GTCATAATAC TAGCGATCCT TCTTGGTTTG 
CTGGTTCTGG CTATCTTAAC TTTAGCTTTA TGGAAGTGTG GATTCTTTGA TAGAGCAAGA 
CCTCCTCAGG ATGAAATGAC AGACAGGGAA CAACTGACCA GTGACAAGAC CCCAGAGGCG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497539.1
CDS	617..2479
Translation	MMNIFVDLLVGAFGKGKVAVYRARPVVTVDAQLLLHPMIINLENKTCQIPEFPTPVACFSVRVCASIAGQSISNTIALLAEVQLDFLKQKGAIKRTLFLHNHQSHFTFPFVMKQQKSLHCQDFMVYLRDETEFRDKLSPINISLNYSLDDSTFKDSLEVKPILNHYRDNVVTEQAHILVDCGEDNLCVPDLKLSARPDKHQIIIGDENHLMLIINARNEGEGAYEAELFVIIPEEADYVGIERNNKGLRPLSCEYKMENVTRMVVCDLGNPMVTGTNFSLGLRFAVPRLEKTNMSINFDLQIRSSNKDNPDSNFERVQINITAIAQVEIRGVSHPPQIVLPIHNWEPEKKPHKEEEVGPLVEHIYELHNIGPSTISDSILDVGWPFSARDEFLLYIFHLQTLGPLQCQTNPEINPQDIKPAASPEDTPELSAFLRNATIPHLVRKRDVPVVQLHRQSPARILNCTNIDCLQISCAVGRLGGGESAVLKVRSRLWAHTFLKEISVEETSQEQLVKTLKRKNDHYALASLVSFEVKKMPYKEQPAKLPAGSTAVKTSVIWATPNVSFSIPLWVIILAILLGLLVLAILTLALWKCGFFDRARPPQDEMTDREQLTSDKTPEA

Target ORF information:

RefSeq Version	XM_006497476.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus integrin alpha 8 (Itga8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497476.3

ORF Insert Sequence:

ATGATGAACA TCTTTGTAGA TTTGCTTGTA GGGGCATTTG GAAAAGGGAA AGTAGCTGTT 
TACAGAGCAA GGCCAGTTGT AACCGTGGAT GCCCAGCTTC TGCTGCACCC GATGATTATC 
AACCTTGAAA ATAAAACTTG CCAGATTCCA GAATTCCCTA CTCCTGTGGC CTGCTTTTCT 
GTAAGAGTCT GTGCATCTAT AGCAGGCCAG AGCATTTCAA ATACAATAGC CCTGCTGGCC 
GAGGTGCAGT TAGATTTCCT GAAGCAAAAA GGAGCCATCA AACGGACGCT CTTTCTCCAC 
AACCACCAGT CCCATTTCAC CTTCCCCTTT GTGATGAAGC AGCAGAAATC CCTCCACTGC 
CAGGATTTTA TGGTTTACCT TCGGGATGAA ACTGAATTCC GAGATAAATT GTCTCCAATC 
AACATCAGCC TGAACTACAG TTTGGATGAT TCTACCTTTA AAGACAGCCT GGAAGTGAAG 
CCAATTTTGA ACCACTACAG GGACAATGTA GTTACTGAGC AGGCTCACAT CCTGGTGGAC 
TGTGGAGAAG ACAATCTATG CGTTCCTGAC TTGAAGCTGT CAGCTAGACC AGATAAGCAT 
CAGATAATTA TTGGCGATGA AAATCACCTA ATGCTCATAA TAAATGCAAG AAATGAGGGA 
GAAGGGGCCT ACGAAGCCGA ACTCTTTGTT ATCATCCCCG AAGAAGCAGA TTATGTTGGG 
ATTGAGCGCA ACAACAAGGG ACTGAGGCCC CTGAGTTGTG AGTACAAGAT GGAAAACGTG 
ACCAGGATGG TGGTGTGTGA CCTTGGGAAC CCGATGGTGA CTGGAACAAA TTTTTCTCTG 
GGCCTCCGAT TTGCTGTTCC TCGCCTTGAG AAAACAAATA TGAGCATTAA CTTCGATCTC 
CAAATCAGAA GCTCCAATAA GGACAATCCT GACAGCAACT TCGAGCGTGT ACAGATCAAC 
ATCACTGCCA TTGCTCAGGT CGAAATCAGA GGAGTGTCCC ACCCTCCGCA GATTGTTCTG 
CCCATCCACA ACTGGGAACC AGAAAAGAAG CCACACAAAG AGGAGGAAGT CGGTCCTTTG 
GTTGAGCATA TTTATGAGCT GCACAATATT GGACCCAGCA CCATCAGTGA CAGCATTCTT 
GACGTGGGCT GGCCGTTCTC TGCACGGGAT GAGTTTCTCC TCTATATTTT TCATCTTCAA 
ACTCTGGGAC CTCTACAATG TCAAACAAAT CCTGAGATCA ACCCACAGGA TATAAAGCCT 
GCTGCCTCCC CAGAGGATAC CCCTGAGCTC AGTGCCTTCT TAAGAAATGC TACAATTCCC 
CATCTTGTCA GAAAGAGGGA TGTGCCAGTG GTCCAGCTCC ACAGACAGAG CCCTGCAAGA 
ATACTGAACT GTACCAACAT TGACTGCTTG CAAATCTCTT GTGCAGTGGG TCGCCTGGGA 
GGAGGCGAAA GTGCAGTCCT AAAGGTCAGA TCGAGATTGT GGGCCCACAC GTTCCTCAAG 
GAAATTTCTG TAGAAGAGAC GAGCCAAGAG CAGTTGGTGA AGACACTCAA GAGAAAGAAT 
GATCATTATG CTCTTGCATC CCTGGTGTCC TTTGAAGTCA AGAAGATGCC GTATAAAGAA 
CAGCCAGCAA AGCTGCCAGC GGGGAGCACA GCGGTTAAGA CGTCGGTTAT TTGGGCCACT 
CCGAATGTCT CCTTCTCAAT TCCATTGTGG GTCATAATAC TAGCGATCCT TCTTGGTTTG 
CTGGTTCTGG CTATCTTAAC TTTAGCTTTA TGGAAGTGTG GATTCTTTGA TAGAGCAAGA 
CCTCCTCAGG ATGAAATGAC AGACAGGGAA CAACTGACCA GTGACAAGAC CCCAGAGGCG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Itga8 ( NM_001001309.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001001309.1 Mus musculus integrin alpha 8 (Itga8), mRNA.

Price & Availability↑

CloneID	OMu20099
Clone ID Related Accession (Same CDS sequence)	NM_001001309.2 , NM_001001309.3
Accession Version	NM_001001309.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3189bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-14
Organism	Mus musculus(House mouse)
Product	integrin alpha-8 precursor
Comment

ATGTCTGCGG GAACCCACTG TGGTCCCCCG GGGAACCGGG CACCTCCGTT CGCGCGTCTC 
TGTTGCGTCT CGGCCGCGCT GGGGATGCTG TGGTCCCCTG CGTGTCTGGC GTTCAACTTG 
GATGTGGACA AGCTCACTGT GTACAGTGGC CCCGAGGGCA GCTACTTCGG CTACTCACTG 
GACTTCTACA TACCTGATGC GCGCACAGCC AGTGTCTTAG TGGGGGCACC CAAAGCCAAC 
ACTAGCCAGC CGGATATCGT AGAAGGGGGA GCTGTCTACT ACTGTCCCTG GCCCTCAGAG 
AGGTCTGCAC AGTGCAAGCA GATACCGTTT GACACCACCA ACAACAGGAA AATCAGAGTT 
AATGGAACCA AAGAACCTAT AGAATTTAAA TCAAACCAGT GGTTTGGAGC CACAGTGAGA 
GCTCACAAAG GAAAAGTTGT GGCTTGTGCC CCTTTGTATC ACTGGAGAAC TCTGAAACCT 
AATCCAGCGA AGGACCCAGT TGGCACATGC TATGTAGCAA TTCAGAATTT CAGTGCCTAT 
GCTGAGCACT CACCTTGTCG AAACAGCAAT GCTGATCCCG AAGGCCAAGG TTACTGCCAG 
GCAGGGTTTA GCCTAGACTT CTATAAGAAT GGAGACCTTA TAGTGGGAGG ACCTGGAAGT 
TTTTACTGGC AAGGTCAGGT GATCACTGTC AGTATAGCAG ACATCATTGC AAATTACTCA 
TTCAAGGATA TTCTACGAAA ATTGGCCGCA GAAAAGCAGA CAGACGTGGC TCCAGCTTCC 
TATGATGACA GCTACCTTGG ATATTCGGTC GCTGCTGGAG AATTCACTGG GGACTCTCAG 
CAAGAATTGG TGGCTGGGAT TCCAAGAGGA GCACAGAACT TTGGATATGT CTCCATCATT 
AACTCCACAG ACATGACCTT CATTCAGAAT TTTACTGGTG AGCAGATGGC ATCTTATTTC 
GGATATACTG TTGTGGTATC AGATGTTAAC AATGATGGCA TGGATGACAT ATTGGTCGGG 
GCACCTCTCT TTATGGAGCG AGAATTTGAA AGTAACCCTA GAGAAGTGGG ACAGGTCTAC 
TTGTACCTTC AAGCAAGCGC CCTCCTCTTC CAAGACCCAC AGGTCCTCAC CGGCACGGAG 
ACATTTGGGA GATTTGGTAG CTCTGTGGCC CACTTGGGGG ACCTGAACCA AGATGGATAC 
AATGACATCG CCATCGGTGT GCCCTTTGCA GGCAAGGATC AACGAGGTAA AGTCCTCATT 
TACAATGGAA ACCCAAGAGG TTTACACAGC AAGCCTTCCC AAGTTCTGCA AGGAATATGG 
GGGTCACAGA CCATCCCTTC TGGATTTGGC TTTTCTCTAA GAGGAGATGC AGACATAGAC 
AAGAATGATT ACCCAGATTT GCTTGTAGGG GCATTTGGAA AAGGGAAAGT AGCTGTTTAC 
AGAGCAAGGC CAGTTGTAAC CGTGGATGCC CAGCTTCTGC TGCACCCGAT GATTATCAAC 
CTTGAAAATA AAACTTGCCA GATTCCAGAA TTCCCTACTC CTGTGGCCTG CTTTTCTGTA 
AGAGTCTGTG CATCTATAGC AGGCCAGAGC ATTTCAAATA CAATAGCCCT GCTGGCCGAG 
GTGCAGTTAG ATTTCCTGAA GCAAAAAGGA GCCATCAAAC GGACGCTCTT TCTCCACAAC 
CACCAGTCCC ATTTCACCTT CCCCTTTGTG ATGAAGCAGC AGAAATCCCT CCACTGCCAG 
GATTTTATGG TTTACCTTCG GGATGAAACT GAATTCCGAG ATAAATTGTC TCCAATCAAC 
ATCAGCCTGA ACTACAGTTT GGATGATTCT ACCTTTAAAG ACAGCCTGGA AGTGAAGCCA 
ATTTTGAACC ACTACAGGGA CAATGTAGTT ACTGAGCAGG CTCACATCCT GGTGGACTGT 
GGAGAAGACA ATCTATGCGT TCCTGACTTG AAGCTGTCAG CTAGACCAGA TAAGCATCAG 
ATAATTATTG GCGATGAAAA TCACCTAATG CTCATAATAA ATGCAAGAAA TGAGGGAGAA 
GGGGCCTACG AAGCCGAACT CTTTGTTATC ATCCCCGAAG AAGCAGATTA TGTTGGGATT 
GAGCGCAACA ACAAGGGACT GAGGCCCCTG AGTTGTGAGT ACAAGATGGA AAACGTGACC 
AGGATGGTGG TGTGTGACCT TGGGAACCCG ATGGTGACTG GAACAAATTT TTCTCTGGGC 
CTCCGATTTG CTGTTCCTCG CCTTGAGAAA ACAAATATGA GCATTAACTT CGATCTCCAA 
ATCAGAAGCT CCAATAAGGA CAATCCTGAC AGCAACTTCG AGCGTGTACA GATCAACATC 
ACTGCCATTG CTCAGGTCGA AATCAGAGGA GTGTCCCACC CTCCGCAGAT TGTTCTGCCC 
ATCCACAACT GGGAACCAGA AAAGAAGCCA CACAAAGAGG AGGAAGTCGG TCCTTTGGTT 
GAGCATATTT ATGAGCTGCA CAATATTGGA CCCAGCACCA TCAGTGACAG CATTCTTGAC 
GTGGGCTGGC CGTTCTCTGC ACGGGATGAG TTTCTCCTCT ATATTTTTCA TCTTCAAACT 
CTGGGACCTC TACAATGTCA AACAAATCCT GAGATCAACC CACAGGATAT AAAGCCTGCT 
GCCTCCCCAG AGGATACCCC TGAGCTCAGT GCCTTCTTAA GAAATGCTAC AATTCCCCAT 
CTTGTCAGAA AGAGGGATGT GCCAGTGGTC CAGCTCCACA GACAGAGCCC TGCAAGAATA 
CTGAACTGTA CCAACATTGA CTGCTTGCAA ATCTCTTGTG CAGTGGGTCG CCTGGGAGGA 
GGCGAAAGTG CAGTCCTAAA GGTCAGATCG AGATTGTGGG CCCACACGTT CCTCAAGAGA 
AAGAATGATC ATTATGCTCT TGCATCCCTG GTGTCCTTTG AAGTCAAGAA GATGCCGTAT 
AAAGAACAGC CAGCAAAGCT GCCAGCGGGG AGCACAGCGG TTAAGACGTC GGTTATTTGG 
GCCACTCCGA ATGTCTCCTT CTCAATTCCA TTGTGGGTCA TAATACTAGC GATCCTTCTT 
GGTTTGCTGG TTCTGGCTAT CTTAACTTTA GCTTTATGGA AGTGTGGATT CTTTGATAGA 
GCAAGACCTC CTCAGGATGA AATGACAGAC AGGGAACAAC TGACCAGTGA CAAGACCCCA 
GAGGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_001001309.1

Translation

MSAGTHCGPPGNRAPPFARLCCVSAALGMLWSPACLAFNLDVDKLTVYSGPEGSYFGYSLDFYIPDARTASVLVGAPKANTSQPDIVEGGAVYYCPWPSERSAQCKQIPFDTTNNRKIRVNGTKEPIEFKSNQWFGATVRAHKGKVVACAPLYHWRTLKPNPAKDPVGTCYVAIQNFSAYAEHSPCRNSNADPEGQGYCQAGFSLDFYKNGDLIVGGPGSFYWQGQVITVSIADIIANYSFKDILRKLAAEKQTDVAPASYDDSYLGYSVAAGEFTGDSQQELVAGIPRGAQNFGYVSIINSTDMTFIQNFTGEQMASYFGYTVVVSDVNNDGMDDILVGAPLFMEREFESNPREVGQVYLYLQASALLFQDPQVLTGTETFGRFGSSVAHLGDLNQDGYNDIAIGVPFAGKDQRGKVLIYNGNPRGLHSKPSQVLQGIWGSQTIPSGFGFSLRGDADIDKNDYPDLLVGAFGKGKVAVYRARPVVTVDAQLLLHPMIINLENKTCQIPEFPTPVACFSVRVCASIAGQSISNTIALLAEVQLDFLKQKGAIKRTLFLHNHQSHFTFPFVMKQQKSLHCQDFMVYLRDETEFRDKLSPINISLNYSLDDSTFKDSLEVKPILNHYRDNVVTEQAHILVDCGEDNLCVPDLKLSARPDKHQIIIGDENHLMLIINARNEGEGAYEAELFVIIPEEADYVGIERNNKGLRPLSCEYKMENVTRMVVCDLGNPMVTGTNFSLGLRFAVPRLEKTNMSINFDLQIRSSNKDNPDSNFERVQINITAIAQVEIRGVSHPPQIVLPIHNWEPEKKPHKEEEVGPLVEHIYELHNIGPSTISDSILDVGWPFSARDEFLLYIFHLQTLGPLQCQTNPEINPQDIKPAASPEDTPELSAFLRNATIPHLVRKRDVPVVQLHRQSPARILNCTNIDCLQISCAVGRLGGGESAVLKVRSRLWAHTFLKRKNDHYALASLVSFEVKKMPYKEQPAKLPAGSTAVKTSVIWATPNVSFSIPLWVIILAILLGLLVLAILTLALWKCGFFDRARPPQDEMTDREQLTSDKTPEA

Target ORF information:

RefSeq Version	NM_001001309.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus integrin alpha 8 (Itga8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001001309.2

ORF Insert Sequence:

ATGTCTGCGG GAACCCACTG TGGTCCCCCG GGGAACCGGG CACCTCCGTT CGCGCGTCTC 
TGTTGCGTCT CGGCCGCGCT GGGGATGCTG TGGTCCCCTG CGTGTCTGGC GTTCAACTTG 
GATGTGGACA AGCTCACTGT GTACAGTGGC CCCGAGGGCA GCTACTTCGG CTACTCACTG 
GACTTCTACA TACCTGATGC GCGCACAGCC AGTGTCTTAG TGGGGGCACC CAAAGCCAAC 
ACTAGCCAGC CGGATATCGT AGAAGGGGGA GCTGTCTACT ACTGTCCCTG GCCCTCAGAG 
AGGTCTGCAC AGTGCAAGCA GATACCGTTT GACACCACCA ACAACAGGAA AATCAGAGTT 
AATGGAACCA AAGAACCTAT AGAATTTAAA TCAAACCAGT GGTTTGGAGC CACAGTGAGA 
GCTCACAAAG GAAAAGTTGT GGCTTGTGCC CCTTTGTATC ACTGGAGAAC TCTGAAACCT 
AATCCAGCGA AGGACCCAGT TGGCACATGC TATGTAGCAA TTCAGAATTT CAGTGCCTAT 
GCTGAGCACT CACCTTGTCG AAACAGCAAT GCTGATCCCG AAGGCCAAGG TTACTGCCAG 
GCAGGGTTTA GCCTAGACTT CTATAAGAAT GGAGACCTTA TAGTGGGAGG ACCTGGAAGT 
TTTTACTGGC AAGGTCAGGT GATCACTGTC AGTATAGCAG ACATCATTGC AAATTACTCA 
TTCAAGGATA TTCTACGAAA ATTGGCCGCA GAAAAGCAGA CAGACGTGGC TCCAGCTTCC 
TATGATGACA GCTACCTTGG ATATTCGGTC GCTGCTGGAG AATTCACTGG GGACTCTCAG 
CAAGAATTGG TGGCTGGGAT TCCAAGAGGA GCACAGAACT TTGGATATGT CTCCATCATT 
AACTCCACAG ACATGACCTT CATTCAGAAT TTTACTGGTG AGCAGATGGC ATCTTATTTC 
GGATATACTG TTGTGGTATC AGATGTTAAC AATGATGGCA TGGATGACAT ATTGGTCGGG 
GCACCTCTCT TTATGGAGCG AGAATTTGAA AGTAACCCTA GAGAAGTGGG ACAGGTCTAC 
TTGTACCTTC AAGCAAGCGC CCTCCTCTTC CAAGACCCAC AGGTCCTCAC CGGCACGGAG 
ACATTTGGGA GATTTGGTAG CTCTGTGGCC CACTTGGGGG ACCTGAACCA AGATGGATAC 
AATGACATCG CCATCGGTGT GCCCTTTGCA GGCAAGGATC AACGAGGTAA AGTCCTCATT 
TACAATGGAA ACCCAAGAGG TTTACACAGC AAGCCTTCCC AAGTTCTGCA AGGAATATGG 
GGGTCACAGA CCATCCCTTC TGGATTTGGC TTTTCTCTAA GAGGAGATGC AGACATAGAC 
AAGAATGATT ACCCAGATTT GCTTGTAGGG GCATTTGGAA AAGGGAAAGT AGCTGTTTAC 
AGAGCAAGGC CAGTTGTAAC CGTGGATGCC CAGCTTCTGC TGCACCCGAT GATTATCAAC 
CTTGAAAATA AAACTTGCCA GATTCCAGAA TTCCCTACTC CTGTGGCCTG CTTTTCTGTA 
AGAGTCTGTG CATCTATAGC AGGCCAGAGC ATTTCAAATA CAATAGCCCT GCTGGCCGAG 
GTGCAGTTAG ATTTCCTGAA GCAAAAAGGA GCCATCAAAC GGACGCTCTT TCTCCACAAC 
CACCAGTCCC ATTTCACCTT CCCCTTTGTG ATGAAGCAGC AGAAATCCCT CCACTGCCAG 
GATTTTATGG TTTACCTTCG GGATGAAACT GAATTCCGAG ATAAATTGTC TCCAATCAAC 
ATCAGCCTGA ACTACAGTTT GGATGATTCT ACCTTTAAAG ACAGCCTGGA AGTGAAGCCA 
ATTTTGAACC ACTACAGGGA CAATGTAGTT ACTGAGCAGG CTCACATCCT GGTGGACTGT 
GGAGAAGACA ATCTATGCGT TCCTGACTTG AAGCTGTCAG CTAGACCAGA TAAGCATCAG 
ATAATTATTG GCGATGAAAA TCACCTAATG CTCATAATAA ATGCAAGAAA TGAGGGAGAA 
GGGGCCTACG AAGCCGAACT CTTTGTTATC ATCCCCGAAG AAGCAGATTA TGTTGGGATT 
GAGCGCAACA ACAAGGGACT GAGGCCCCTG AGTTGTGAGT ACAAGATGGA AAACGTGACC 
AGGATGGTGG TGTGTGACCT TGGGAACCCG ATGGTGACTG GAACAAATTT TTCTCTGGGC 
CTCCGATTTG CTGTTCCTCG CCTTGAGAAA ACAAATATGA GCATTAACTT CGATCTCCAA 
ATCAGAAGCT CCAATAAGGA CAATCCTGAC AGCAACTTCG AGCGTGTACA GATCAACATC 
ACTGCCATTG CTCAGGTCGA AATCAGAGGA GTGTCCCACC CTCCGCAGAT TGTTCTGCCC 
ATCCACAACT GGGAACCAGA AAAGAAGCCA CACAAAGAGG AGGAAGTCGG TCCTTTGGTT 
GAGCATATTT ATGAGCTGCA CAATATTGGA CCCAGCACCA TCAGTGACAG CATTCTTGAC 
GTGGGCTGGC CGTTCTCTGC ACGGGATGAG TTTCTCCTCT ATATTTTTCA TCTTCAAACT 
CTGGGACCTC TACAATGTCA AACAAATCCT GAGATCAACC CACAGGATAT AAAGCCTGCT 
GCCTCCCCAG AGGATACCCC TGAGCTCAGT GCCTTCTTAA GAAATGCTAC AATTCCCCAT 
CTTGTCAGAA AGAGGGATGT GCCAGTGGTC CAGCTCCACA GACAGAGCCC TGCAAGAATA 
CTGAACTGTA CCAACATTGA CTGCTTGCAA ATCTCTTGTG CAGTGGGTCG CCTGGGAGGA 
GGCGAAAGTG CAGTCCTAAA GGTCAGATCG AGATTGTGGG CCCACACGTT CCTCAAGAGA 
AAGAATGATC ATTATGCTCT TGCATCCCTG GTGTCCTTTG AAGTCAAGAA GATGCCGTAT 
AAAGAACAGC CAGCAAAGCT GCCAGCGGGG AGCACAGCGG TTAAGACGTC GGTTATTTGG 
GCCACTCCGA ATGTCTCCTT CTCAATTCCA TTGTGGGTCA TAATACTAGC GATCCTTCTT 
GGTTTGCTGG TTCTGGCTAT CTTAACTTTA GCTTTATGGA AGTGTGGATT CTTTGATAGA 
GCAAGACCTC CTCAGGATGA AATGACAGAC AGGGAACAAC TGACCAGTGA CAAGACCCCA 
GAGGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Itga8 ( NM_001001309.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001001309.1 Mus musculus integrin alpha 8 (Itga8), transcript variant 1, mRNA.

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CloneID	OMu20099
Clone ID Related Accession (Same CDS sequence)	NM_001001309.2 , NM_001001309.3
Accession Version	NM_001001309.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3189bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	integrin alpha-8 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL845313.9, AK044910.1, AK135193.1, BC137793.1, AK033007.1 and BQ175493.1. On Aug 18, 2016 this sequence version replaced NM_001001309.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF041409.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGTCTGCGG GAACCCACTG TGGTCCCCCG GGGAACCGGG CACCTCCGTT CGCGCGTCTC 
TGTTGCGTCT CGGCCGCGCT GGGGATGCTG TGGTCCCCTG CGTGTCTGGC GTTCAACTTG 
GATGTGGACA AGCTCACTGT GTACAGTGGC CCCGAGGGCA GCTACTTCGG CTACTCACTG 
GACTTCTACA TACCTGATGC GCGCACAGCC AGTGTCTTAG TGGGGGCACC CAAAGCCAAC 
ACTAGCCAGC CGGATATCGT AGAAGGGGGA GCTGTCTACT ACTGTCCCTG GCCCTCAGAG 
AGGTCTGCAC AGTGCAAGCA GATACCGTTT GACACCACCA ACAACAGGAA AATCAGAGTT 
AATGGAACCA AAGAACCTAT AGAATTTAAA TCAAACCAGT GGTTTGGAGC CACAGTGAGA 
GCTCACAAAG GAAAAGTTGT GGCTTGTGCC CCTTTGTATC ACTGGAGAAC TCTGAAACCT 
AATCCAGCGA AGGACCCAGT TGGCACATGC TATGTAGCAA TTCAGAATTT CAGTGCCTAT 
GCTGAGCACT CACCTTGTCG AAACAGCAAT GCTGATCCCG AAGGCCAAGG TTACTGCCAG 
GCAGGGTTTA GCCTAGACTT CTATAAGAAT GGAGACCTTA TAGTGGGAGG ACCTGGAAGT 
TTTTACTGGC AAGGTCAGGT GATCACTGTC AGTATAGCAG ACATCATTGC AAATTACTCA 
TTCAAGGATA TTCTACGAAA ATTGGCCGCA GAAAAGCAGA CAGACGTGGC TCCAGCTTCC 
TATGATGACA GCTACCTTGG ATATTCGGTC GCTGCTGGAG AATTCACTGG GGACTCTCAG 
CAAGAATTGG TGGCTGGGAT TCCAAGAGGA GCACAGAACT TTGGATATGT CTCCATCATT 
AACTCCACAG ACATGACCTT CATTCAGAAT TTTACTGGTG AGCAGATGGC ATCTTATTTC 
GGATATACTG TTGTGGTATC AGATGTTAAC AATGATGGCA TGGATGACAT ATTGGTCGGG 
GCACCTCTCT TTATGGAGCG AGAATTTGAA AGTAACCCTA GAGAAGTGGG ACAGGTCTAC 
TTGTACCTTC AAGCAAGCGC CCTCCTCTTC CAAGACCCAC AGGTCCTCAC CGGCACGGAG 
ACATTTGGGA GATTTGGTAG CTCTGTGGCC CACTTGGGGG ACCTGAACCA AGATGGATAC 
AATGACATCG CCATCGGTGT GCCCTTTGCA GGCAAGGATC AACGAGGTAA AGTCCTCATT 
TACAATGGAA ACCCAAGAGG TTTACACAGC AAGCCTTCCC AAGTTCTGCA AGGAATATGG 
GGGTCACAGA CCATCCCTTC TGGATTTGGC TTTTCTCTAA GAGGAGATGC AGACATAGAC 
AAGAATGATT ACCCAGATTT GCTTGTAGGG GCATTTGGAA AAGGGAAAGT AGCTGTTTAC 
AGAGCAAGGC CAGTTGTAAC CGTGGATGCC CAGCTTCTGC TGCACCCGAT GATTATCAAC 
CTTGAAAATA AAACTTGCCA GATTCCAGAA TTCCCTACTC CTGTGGCCTG CTTTTCTGTA 
AGAGTCTGTG CATCTATAGC AGGCCAGAGC ATTTCAAATA CAATAGCCCT GCTGGCCGAG 
GTGCAGTTAG ATTTCCTGAA GCAAAAAGGA GCCATCAAAC GGACGCTCTT TCTCCACAAC 
CACCAGTCCC ATTTCACCTT CCCCTTTGTG ATGAAGCAGC AGAAATCCCT CCACTGCCAG 
GATTTTATGG TTTACCTTCG GGATGAAACT GAATTCCGAG ATAAATTGTC TCCAATCAAC 
ATCAGCCTGA ACTACAGTTT GGATGATTCT ACCTTTAAAG ACAGCCTGGA AGTGAAGCCA 
ATTTTGAACC ACTACAGGGA CAATGTAGTT ACTGAGCAGG CTCACATCCT GGTGGACTGT 
GGAGAAGACA ATCTATGCGT TCCTGACTTG AAGCTGTCAG CTAGACCAGA TAAGCATCAG 
ATAATTATTG GCGATGAAAA TCACCTAATG CTCATAATAA ATGCAAGAAA TGAGGGAGAA 
GGGGCCTACG AAGCCGAACT CTTTGTTATC ATCCCCGAAG AAGCAGATTA TGTTGGGATT 
GAGCGCAACA ACAAGGGACT GAGGCCCCTG AGTTGTGAGT ACAAGATGGA AAACGTGACC 
AGGATGGTGG TGTGTGACCT TGGGAACCCG ATGGTGACTG GAACAAATTT TTCTCTGGGC 
CTCCGATTTG CTGTTCCTCG CCTTGAGAAA ACAAATATGA GCATTAACTT CGATCTCCAA 
ATCAGAAGCT CCAATAAGGA CAATCCTGAC AGCAACTTCG AGCGTGTACA GATCAACATC 
ACTGCCATTG CTCAGGTCGA AATCAGAGGA GTGTCCCACC CTCCGCAGAT TGTTCTGCCC 
ATCCACAACT GGGAACCAGA AAAGAAGCCA CACAAAGAGG AGGAAGTCGG TCCTTTGGTT 
GAGCATATTT ATGAGCTGCA CAATATTGGA CCCAGCACCA TCAGTGACAG CATTCTTGAC 
GTGGGCTGGC CGTTCTCTGC ACGGGATGAG TTTCTCCTCT ATATTTTTCA TCTTCAAACT 
CTGGGACCTC TACAATGTCA AACAAATCCT GAGATCAACC CACAGGATAT AAAGCCTGCT 
GCCTCCCCAG AGGATACCCC TGAGCTCAGT GCCTTCTTAA GAAATGCTAC AATTCCCCAT 
CTTGTCAGAA AGAGGGATGT GCCAGTGGTC CAGCTCCACA GACAGAGCCC TGCAAGAATA 
CTGAACTGTA CCAACATTGA CTGCTTGCAA ATCTCTTGTG CAGTGGGTCG CCTGGGAGGA 
GGCGAAAGTG CAGTCCTAAA GGTCAGATCG AGATTGTGGG CCCACACGTT CCTCAAGAGA 
AAGAATGATC ATTATGCTCT TGCATCCCTG GTGTCCTTTG AAGTCAAGAA GATGCCGTAT 
AAAGAACAGC CAGCAAAGCT GCCAGCGGGG AGCACAGCGG TTAAGACGTC GGTTATTTGG 
GCCACTCCGA ATGTCTCCTT CTCAATTCCA TTGTGGGTCA TAATACTAGC GATCCTTCTT 
GGTTTGCTGG TTCTGGCTAT CTTAACTTTA GCTTTATGGA AGTGTGGATT CTTTGATAGA 
GCAAGACCTC CTCAGGATGA AATGACAGAC AGGGAACAAC TGACCAGTGA CAAGACCCCA 
GAGGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001001309.1
CDS	163..3351
Translation	MSAGTHCGPPGNRAPPFARLCCVSAALGMLWSPACLAFNLDVDKLTVYSGPEGSYFGYSLDFYIPDARTASVLVGAPKANTSQPDIVEGGAVYYCPWPSERSAQCKQIPFDTTNNRKIRVNGTKEPIEFKSNQWFGATVRAHKGKVVACAPLYHWRTLKPNPAKDPVGTCYVAIQNFSAYAEHSPCRNSNADPEGQGYCQAGFSLDFYKNGDLIVGGPGSFYWQGQVITVSIADIIANYSFKDILRKLAAEKQTDVAPASYDDSYLGYSVAAGEFTGDSQQELVAGIPRGAQNFGYVSIINSTDMTFIQNFTGEQMASYFGYTVVVSDVNNDGMDDILVGAPLFMEREFESNPREVGQVYLYLQASALLFQDPQVLTGTETFGRFGSSVAHLGDLNQDGYNDIAIGVPFAGKDQRGKVLIYNGNPRGLHSKPSQVLQGIWGSQTIPSGFGFSLRGDADIDKNDYPDLLVGAFGKGKVAVYRARPVVTVDAQLLLHPMIINLENKTCQIPEFPTPVACFSVRVCASIAGQSISNTIALLAEVQLDFLKQKGAIKRTLFLHNHQSHFTFPFVMKQQKSLHCQDFMVYLRDETEFRDKLSPINISLNYSLDDSTFKDSLEVKPILNHYRDNVVTEQAHILVDCGEDNLCVPDLKLSARPDKHQIIIGDENHLMLIINARNEGEGAYEAELFVIIPEEADYVGIERNNKGLRPLSCEYKMENVTRMVVCDLGNPMVTGTNFSLGLRFAVPRLEKTNMSINFDLQIRSSNKDNPDSNFERVQINITAIAQVEIRGVSHPPQIVLPIHNWEPEKKPHKEEEVGPLVEHIYELHNIGPSTISDSILDVGWPFSARDEFLLYIFHLQTLGPLQCQTNPEINPQDIKPAASPEDTPELSAFLRNATIPHLVRKRDVPVVQLHRQSPARILNCTNIDCLQISCAVGRLGGGESAVLKVRSRLWAHTFLKRKNDHYALASLVSFEVKKMPYKEQPAKLPAGSTAVKTSVIWATPNVSFSIPLWVIILAILLGLLVLAILTLALWKCGFFDRARPPQDEMTDREQLTSDKTPEA

Target ORF information:

RefSeq Version	NM_001001309.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus integrin alpha 8 (Itga8), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001001309.3

ORF Insert Sequence:

ATGTCTGCGG GAACCCACTG TGGTCCCCCG GGGAACCGGG CACCTCCGTT CGCGCGTCTC 
TGTTGCGTCT CGGCCGCGCT GGGGATGCTG TGGTCCCCTG CGTGTCTGGC GTTCAACTTG 
GATGTGGACA AGCTCACTGT GTACAGTGGC CCCGAGGGCA GCTACTTCGG CTACTCACTG 
GACTTCTACA TACCTGATGC GCGCACAGCC AGTGTCTTAG TGGGGGCACC CAAAGCCAAC 
ACTAGCCAGC CGGATATCGT AGAAGGGGGA GCTGTCTACT ACTGTCCCTG GCCCTCAGAG 
AGGTCTGCAC AGTGCAAGCA GATACCGTTT GACACCACCA ACAACAGGAA AATCAGAGTT 
AATGGAACCA AAGAACCTAT AGAATTTAAA TCAAACCAGT GGTTTGGAGC CACAGTGAGA 
GCTCACAAAG GAAAAGTTGT GGCTTGTGCC CCTTTGTATC ACTGGAGAAC TCTGAAACCT 
AATCCAGCGA AGGACCCAGT TGGCACATGC TATGTAGCAA TTCAGAATTT CAGTGCCTAT 
GCTGAGCACT CACCTTGTCG AAACAGCAAT GCTGATCCCG AAGGCCAAGG TTACTGCCAG 
GCAGGGTTTA GCCTAGACTT CTATAAGAAT GGAGACCTTA TAGTGGGAGG ACCTGGAAGT 
TTTTACTGGC AAGGTCAGGT GATCACTGTC AGTATAGCAG ACATCATTGC AAATTACTCA 
TTCAAGGATA TTCTACGAAA ATTGGCCGCA GAAAAGCAGA CAGACGTGGC TCCAGCTTCC 
TATGATGACA GCTACCTTGG ATATTCGGTC GCTGCTGGAG AATTCACTGG GGACTCTCAG 
CAAGAATTGG TGGCTGGGAT TCCAAGAGGA GCACAGAACT TTGGATATGT CTCCATCATT 
AACTCCACAG ACATGACCTT CATTCAGAAT TTTACTGGTG AGCAGATGGC ATCTTATTTC 
GGATATACTG TTGTGGTATC AGATGTTAAC AATGATGGCA TGGATGACAT ATTGGTCGGG 
GCACCTCTCT TTATGGAGCG AGAATTTGAA AGTAACCCTA GAGAAGTGGG ACAGGTCTAC 
TTGTACCTTC AAGCAAGCGC CCTCCTCTTC CAAGACCCAC AGGTCCTCAC CGGCACGGAG 
ACATTTGGGA GATTTGGTAG CTCTGTGGCC CACTTGGGGG ACCTGAACCA AGATGGATAC 
AATGACATCG CCATCGGTGT GCCCTTTGCA GGCAAGGATC AACGAGGTAA AGTCCTCATT 
TACAATGGAA ACCCAAGAGG TTTACACAGC AAGCCTTCCC AAGTTCTGCA AGGAATATGG 
GGGTCACAGA CCATCCCTTC TGGATTTGGC TTTTCTCTAA GAGGAGATGC AGACATAGAC 
AAGAATGATT ACCCAGATTT GCTTGTAGGG GCATTTGGAA AAGGGAAAGT AGCTGTTTAC 
AGAGCAAGGC CAGTTGTAAC CGTGGATGCC CAGCTTCTGC TGCACCCGAT GATTATCAAC 
CTTGAAAATA AAACTTGCCA GATTCCAGAA TTCCCTACTC CTGTGGCCTG CTTTTCTGTA 
AGAGTCTGTG CATCTATAGC AGGCCAGAGC ATTTCAAATA CAATAGCCCT GCTGGCCGAG 
GTGCAGTTAG ATTTCCTGAA GCAAAAAGGA GCCATCAAAC GGACGCTCTT TCTCCACAAC 
CACCAGTCCC ATTTCACCTT CCCCTTTGTG ATGAAGCAGC AGAAATCCCT CCACTGCCAG 
GATTTTATGG TTTACCTTCG GGATGAAACT GAATTCCGAG ATAAATTGTC TCCAATCAAC 
ATCAGCCTGA ACTACAGTTT GGATGATTCT ACCTTTAAAG ACAGCCTGGA AGTGAAGCCA 
ATTTTGAACC ACTACAGGGA CAATGTAGTT ACTGAGCAGG CTCACATCCT GGTGGACTGT 
GGAGAAGACA ATCTATGCGT TCCTGACTTG AAGCTGTCAG CTAGACCAGA TAAGCATCAG 
ATAATTATTG GCGATGAAAA TCACCTAATG CTCATAATAA ATGCAAGAAA TGAGGGAGAA 
GGGGCCTACG AAGCCGAACT CTTTGTTATC ATCCCCGAAG AAGCAGATTA TGTTGGGATT 
GAGCGCAACA ACAAGGGACT GAGGCCCCTG AGTTGTGAGT ACAAGATGGA AAACGTGACC 
AGGATGGTGG TGTGTGACCT TGGGAACCCG ATGGTGACTG GAACAAATTT TTCTCTGGGC 
CTCCGATTTG CTGTTCCTCG CCTTGAGAAA ACAAATATGA GCATTAACTT CGATCTCCAA 
ATCAGAAGCT CCAATAAGGA CAATCCTGAC AGCAACTTCG AGCGTGTACA GATCAACATC 
ACTGCCATTG CTCAGGTCGA AATCAGAGGA GTGTCCCACC CTCCGCAGAT TGTTCTGCCC 
ATCCACAACT GGGAACCAGA AAAGAAGCCA CACAAAGAGG AGGAAGTCGG TCCTTTGGTT 
GAGCATATTT ATGAGCTGCA CAATATTGGA CCCAGCACCA TCAGTGACAG CATTCTTGAC 
GTGGGCTGGC CGTTCTCTGC ACGGGATGAG TTTCTCCTCT ATATTTTTCA TCTTCAAACT 
CTGGGACCTC TACAATGTCA AACAAATCCT GAGATCAACC CACAGGATAT AAAGCCTGCT 
GCCTCCCCAG AGGATACCCC TGAGCTCAGT GCCTTCTTAA GAAATGCTAC AATTCCCCAT 
CTTGTCAGAA AGAGGGATGT GCCAGTGGTC CAGCTCCACA GACAGAGCCC TGCAAGAATA 
CTGAACTGTA CCAACATTGA CTGCTTGCAA ATCTCTTGTG CAGTGGGTCG CCTGGGAGGA 
GGCGAAAGTG CAGTCCTAAA GGTCAGATCG AGATTGTGGG CCCACACGTT CCTCAAGAGA 
AAGAATGATC ATTATGCTCT TGCATCCCTG GTGTCCTTTG AAGTCAAGAA GATGCCGTAT 
AAAGAACAGC CAGCAAAGCT GCCAGCGGGG AGCACAGCGG TTAAGACGTC GGTTATTTGG 
GCCACTCCGA ATGTCTCCTT CTCAATTCCA TTGTGGGTCA TAATACTAGC GATCCTTCTT 
GGTTTGCTGG TTCTGGCTAT CTTAACTTTA GCTTTATGGA AGTGTGGATT CTTTGATAGA 
GCAAGACCTC CTCAGGATGA AATGACAGAC AGGGAACAAC TGACCAGTGA CAAGACCCCA 
GAGGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Itga8 ( XM_006497475.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497538.1 Mus musculus integrin alpha 8 (Itga8), transcript variant X1, mRNA.

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CloneID	OMu29154
Clone ID Related Accession (Same CDS sequence)	XM_006497475.3 , XM_006497475.4
Accession Version	XM_006497475.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	integrin alpha-8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006497475.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCGG GAACCCACTG TGGTCCCCCG GGGAACCGGG CACCTCCGTT CGCGCGTCTC 
TGTTGCGTCT CGGCCGCGCT GGGGATGCTG TGGTCCCCTG CGTGTCTGGC GTTCAACTTG 
GATGTGGACA AGCTCACTGT GTACAGTGGC CCCGAGGGCA GCTACTTCGG CTACTCACTG 
GACTTCTACA TACCTGATGC GCGCACAGCC AGTGTCTTAG TGGGGGCACC CAAAGCCAAC 
ACTAGCCAGC CGGATATCGT AGAAGGGGGA GCTGTCTACT ACTGTCCCTG GCCCTCAGAG 
AGGTCTGCAC AGTGCAAGCA GATACCGTTT GACACCACCA ACAACAGGAA AATCAGAGTT 
AATGGAACCA AAGAACCTAT AGAATTTAAA TCAAACCAGT GGTTTGGAGC CACAGTGAGA 
GCTCACAAAG GAAAAGTTGT GGCTTGTGCC CCTTTGTATC ACTGGAGAAC TCTGAAACCT 
AATCCAGCGA AGGACCCAGT TGGCACATGC TATGTAGCAA TTCAGAATTT CAGTGCCTAT 
GCTGAGCACT CACCTTGTCG AAACAGCAAT GCTGATCCCG AAGGCCAAGG TTACTGCCAG 
GCAGGGTTTA GCCTAGACTT CTATAAGAAT GGAGACCTTA TAGTGGGAGG ACCTGGAAGT 
TTTTACTGGC AAGGTCAGGT GATCACTGTC AGTATAGCAG ACATCATTGC AAATTACTCA 
TTCAAGGATA TTCTACGAAA ATTGGCCGCA GAAAAGCAGA CAGACGTGGC TCCAGCTTCC 
TATGATGACA GCTACCTTGG ATATTCGGTC GCTGCTGGAG AATTCACTGG GGACTCTCAG 
CAAGAATTGG TGGCTGGGAT TCCAAGAGGA GCACAGAACT TTGGATATGT CTCCATCATT 
AACTCCACAG ACATGACCTT CATTCAGAAT TTTACTGGTG AGCAGATGGC ATCTTATTTC 
GGATATACTG TTGTGGTATC AGATGTTAAC AATGATGGCA TGGATGACAT ATTGGTCGGG 
GCACCTCTCT TTATGGAGCG AGAATTTGAA AGTAACCCTA GAGAAGTGGG ACAGGTCTAC 
TTGTACCTTC AAGCAAGCGC CCTCCTCTTC CAAGACCCAC AGGTCCTCAC CGGCACGGAG 
ACATTTGGGA GATTTGGTAG CTCTGTGGCC CACTTGGGGG ACCTGAACCA AGATGGATAC 
AATGACATCG CCATCGGTGT GCCCTTTGCA GGCAAGGATC AACGAGGTAA AGTCCTCATT 
TACAATGGAA ACCCAAGAGG TTTACACAGC AAGCCTTCCC AAGTTCTGCA AGGAATATGG 
GGGTCACAGA CCATCCCTTC TGGATTTGGC TTTTCTCTAA GAGGAGATGC AGACATAGAC 
AAGAATGATT ACCCAGATTT GCTTGTAGGG GCATTTGGAA AAGGGAAAGT AGCTGTTTAC 
AGAGCAAGGC CAGTTGTAAC CGTGGATGCC CAGCTTCTGC TGCACCCGAT GATTATCAAC 
CTTGAAAATA AAACTTGCCA GATTCCAGAA TTCCCTACTC CTGTGGCCTG CTTTTCTGTA 
AGAGTCTGTG CATCTATAGC AGGCCAGAGC ATTTCAAATA CAATAGCCCT GCTGGCCGAG 
GTGCAGTTAG ATTTCCTGAA GCAAAAAGGA GCCATCAAAC GGACGCTCTT TCTCCACAAC 
CACCAGTCCC ATTTCACCTT CCCCTTTGTG ATGAAGCAGC AGAAATCCCT CCACTGCCAG 
GATTTTATGG TTTACCTTCG GGATGAAACT GAATTCCGAG ATAAATTGTC TCCAATCAAC 
ATCAGCCTGA ACTACAGTTT GGATGATTCT ACCTTTAAAG ACAGCCTGGA AGTGAAGCCA 
ATTTTGAACC ACTACAGGGA CAATGTAGTT ACTGAGCAGG CTCACATCCT GGTGGACTGT 
GGAGAAGACA ATCTATGCGT TCCTGACTTG AAGCTGTCAG CTAGACCAGA TAAGCATCAG 
ATAATTATTG GCGATGAAAA TCACCTAATG CTCATAATAA ATGCAAGAAA TGAGGGAGAA 
GGGGCCTACG AAGCCGAACT CTTTGTTATC ATCCCCGAAG AAGCAGATTA TGTTGGGATT 
GAGCGCAACA ACAAGGGACT GAGGCCCCTG AGTTGTGAGT ACAAGATGGA AAACGTGACC 
AGGATGGTGG TGTGTGACCT TGGGAACCCG ATGGTGACTG GAACAAATTT TTCTCTGGGC 
CTCCGATTTG CTGTTCCTCG CCTTGAGAAA ACAAATATGA GCATTAACTT CGATCTCCAA 
ATCAGAAGCT CCAATAAGGA CAATCCTGAC AGCAACTTCG AGCGTGTACA GATCAACATC 
ACTGCCATTG CTCAGGTCGA AATCAGAGGA GTGTCCCACC CTCCGCAGAT TGTTCTGCCC 
ATCCACAACT GGGAACCAGA AAAGAAGCCA CACAAAGAGG AGGAAGTCGG TCCTTTGGTT 
GAGCATATTT ATGAGCTGCA CAATATTGGA CCCAGCACCA TCAGTGACAG CATTCTTGAC 
GTGGGCTGGC CGTTCTCTGC ACGGGATGAG TTTCTCCTCT ATATTTTTCA TCTTCAAACT 
CTGGGACCTC TACAATGTCA AACAAATCCT GAGATCAACC CACAGGATAT AAAGCCTGCT 
GCCTCCCCAG AGGATACCCC TGAGCTCAGT GCCTTCTTAA GAAATGCTAC AATTCCCCAT 
CTTGTCAGAA AGAGGGATGT GCCAGTGGTC CAGCTCCACA GACAGAGCCC TGCAAGAATA 
CTGAACTGTA CCAACATTGA CTGCTTGCAA ATCTCTTGTG CAGTGGGTCG CCTGGGAGGA 
GGCGAAAGTG CAGTCCTAAA GGTCAGATCG AGATTGTGGG CCCACACGTT CCTCAAGGAA 
ATTTCTGTAG AAGAGACGAG CCAAGAGCAG TTGGTGAAGA CACTCAAGAG AAAGAATGAT 
CATTATGCTC TTGCATCCCT GGTGTCCTTT GAAGTCAAGA AGATGCCGTA TAAAGAACAG 
CCAGCAAAGC TGCCAGCGGG GAGCACAGCG GTTAAGACGT CGGTTATTTG GGCCACTCCG 
AATGTCTCCT TCTCAATTCC ATTGTGGGTC ATAATACTAG CGATCCTTCT TGGTTTGCTG 
GTTCTGGCTA TCTTAACTTT AGCTTTATGG AAGTGTGGAT TCTTTGATAG AGCAAGACCT 
CCTCAGGATG AAATGACAGA CAGGGAACAA CTGACCAGTG ACAAGACCCC AGAGGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497538.1
CDS	204..3443
Translation	MSAGTHCGPPGNRAPPFARLCCVSAALGMLWSPACLAFNLDVDKLTVYSGPEGSYFGYSLDFYIPDARTASVLVGAPKANTSQPDIVEGGAVYYCPWPSERSAQCKQIPFDTTNNRKIRVNGTKEPIEFKSNQWFGATVRAHKGKVVACAPLYHWRTLKPNPAKDPVGTCYVAIQNFSAYAEHSPCRNSNADPEGQGYCQAGFSLDFYKNGDLIVGGPGSFYWQGQVITVSIADIIANYSFKDILRKLAAEKQTDVAPASYDDSYLGYSVAAGEFTGDSQQELVAGIPRGAQNFGYVSIINSTDMTFIQNFTGEQMASYFGYTVVVSDVNNDGMDDILVGAPLFMEREFESNPREVGQVYLYLQASALLFQDPQVLTGTETFGRFGSSVAHLGDLNQDGYNDIAIGVPFAGKDQRGKVLIYNGNPRGLHSKPSQVLQGIWGSQTIPSGFGFSLRGDADIDKNDYPDLLVGAFGKGKVAVYRARPVVTVDAQLLLHPMIINLENKTCQIPEFPTPVACFSVRVCASIAGQSISNTIALLAEVQLDFLKQKGAIKRTLFLHNHQSHFTFPFVMKQQKSLHCQDFMVYLRDETEFRDKLSPINISLNYSLDDSTFKDSLEVKPILNHYRDNVVTEQAHILVDCGEDNLCVPDLKLSARPDKHQIIIGDENHLMLIINARNEGEGAYEAELFVIIPEEADYVGIERNNKGLRPLSCEYKMENVTRMVVCDLGNPMVTGTNFSLGLRFAVPRLEKTNMSINFDLQIRSSNKDNPDSNFERVQINITAIAQVEIRGVSHPPQIVLPIHNWEPEKKPHKEEEVGPLVEHIYELHNIGPSTISDSILDVGWPFSARDEFLLYIFHLQTLGPLQCQTNPEINPQDIKPAASPEDTPELSAFLRNATIPHLVRKRDVPVVQLHRQSPARILNCTNIDCLQISCAVGRLGGGESAVLKVRSRLWAHTFLKEISVEETSQEQLVKTLKRKNDHYALASLVSFEVKKMPYKEQPAKLPAGSTAVKTSVIWATPNVSFSIPLWVIILAILLGLLVLAILTLALWKCGFFDRARPPQDEMTDREQLTSDKTPEA

Target ORF information:

RefSeq Version	XM_006497475.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus integrin alpha 8 (Itga8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497475.4

ORF Insert Sequence:

ATGTCTGCGG GAACCCACTG TGGTCCCCCG GGGAACCGGG CACCTCCGTT CGCGCGTCTC 
TGTTGCGTCT CGGCCGCGCT GGGGATGCTG TGGTCCCCTG CGTGTCTGGC GTTCAACTTG 
GATGTGGACA AGCTCACTGT GTACAGTGGC CCCGAGGGCA GCTACTTCGG CTACTCACTG 
GACTTCTACA TACCTGATGC GCGCACAGCC AGTGTCTTAG TGGGGGCACC CAAAGCCAAC 
ACTAGCCAGC CGGATATCGT AGAAGGGGGA GCTGTCTACT ACTGTCCCTG GCCCTCAGAG 
AGGTCTGCAC AGTGCAAGCA GATACCGTTT GACACCACCA ACAACAGGAA AATCAGAGTT 
AATGGAACCA AAGAACCTAT AGAATTTAAA TCAAACCAGT GGTTTGGAGC CACAGTGAGA 
GCTCACAAAG GAAAAGTTGT GGCTTGTGCC CCTTTGTATC ACTGGAGAAC TCTGAAACCT 
AATCCAGCGA AGGACCCAGT TGGCACATGC TATGTAGCAA TTCAGAATTT CAGTGCCTAT 
GCTGAGCACT CACCTTGTCG AAACAGCAAT GCTGATCCCG AAGGCCAAGG TTACTGCCAG 
GCAGGGTTTA GCCTAGACTT CTATAAGAAT GGAGACCTTA TAGTGGGAGG ACCTGGAAGT 
TTTTACTGGC AAGGTCAGGT GATCACTGTC AGTATAGCAG ACATCATTGC AAATTACTCA 
TTCAAGGATA TTCTACGAAA ATTGGCCGCA GAAAAGCAGA CAGACGTGGC TCCAGCTTCC 
TATGATGACA GCTACCTTGG ATATTCGGTC GCTGCTGGAG AATTCACTGG GGACTCTCAG 
CAAGAATTGG TGGCTGGGAT TCCAAGAGGA GCACAGAACT TTGGATATGT CTCCATCATT 
AACTCCACAG ACATGACCTT CATTCAGAAT TTTACTGGTG AGCAGATGGC ATCTTATTTC 
GGATATACTG TTGTGGTATC AGATGTTAAC AATGATGGCA TGGATGACAT ATTGGTCGGG 
GCACCTCTCT TTATGGAGCG AGAATTTGAA AGTAACCCTA GAGAAGTGGG ACAGGTCTAC 
TTGTACCTTC AAGCAAGCGC CCTCCTCTTC CAAGACCCAC AGGTCCTCAC CGGCACGGAG 
ACATTTGGGA GATTTGGTAG CTCTGTGGCC CACTTGGGGG ACCTGAACCA AGATGGATAC 
AATGACATCG CCATCGGTGT GCCCTTTGCA GGCAAGGATC AACGAGGTAA AGTCCTCATT 
TACAATGGAA ACCCAAGAGG TTTACACAGC AAGCCTTCCC AAGTTCTGCA AGGAATATGG 
GGGTCACAGA CCATCCCTTC TGGATTTGGC TTTTCTCTAA GAGGAGATGC AGACATAGAC 
AAGAATGATT ACCCAGATTT GCTTGTAGGG GCATTTGGAA AAGGGAAAGT AGCTGTTTAC 
AGAGCAAGGC CAGTTGTAAC CGTGGATGCC CAGCTTCTGC TGCACCCGAT GATTATCAAC 
CTTGAAAATA AAACTTGCCA GATTCCAGAA TTCCCTACTC CTGTGGCCTG CTTTTCTGTA 
AGAGTCTGTG CATCTATAGC AGGCCAGAGC ATTTCAAATA CAATAGCCCT GCTGGCCGAG 
GTGCAGTTAG ATTTCCTGAA GCAAAAAGGA GCCATCAAAC GGACGCTCTT TCTCCACAAC 
CACCAGTCCC ATTTCACCTT CCCCTTTGTG ATGAAGCAGC AGAAATCCCT CCACTGCCAG 
GATTTTATGG TTTACCTTCG GGATGAAACT GAATTCCGAG ATAAATTGTC TCCAATCAAC 
ATCAGCCTGA ACTACAGTTT GGATGATTCT ACCTTTAAAG ACAGCCTGGA AGTGAAGCCA 
ATTTTGAACC ACTACAGGGA CAATGTAGTT ACTGAGCAGG CTCACATCCT GGTGGACTGT 
GGAGAAGACA ATCTATGCGT TCCTGACTTG AAGCTGTCAG CTAGACCAGA TAAGCATCAG 
ATAATTATTG GCGATGAAAA TCACCTAATG CTCATAATAA ATGCAAGAAA TGAGGGAGAA 
GGGGCCTACG AAGCCGAACT CTTTGTTATC ATCCCCGAAG AAGCAGATTA TGTTGGGATT 
GAGCGCAACA ACAAGGGACT GAGGCCCCTG AGTTGTGAGT ACAAGATGGA AAACGTGACC 
AGGATGGTGG TGTGTGACCT TGGGAACCCG ATGGTGACTG GAACAAATTT TTCTCTGGGC 
CTCCGATTTG CTGTTCCTCG CCTTGAGAAA ACAAATATGA GCATTAACTT CGATCTCCAA 
ATCAGAAGCT CCAATAAGGA CAATCCTGAC AGCAACTTCG AGCGTGTACA GATCAACATC 
ACTGCCATTG CTCAGGTCGA AATCAGAGGA GTGTCCCACC CTCCGCAGAT TGTTCTGCCC 
ATCCACAACT GGGAACCAGA AAAGAAGCCA CACAAAGAGG AGGAAGTCGG TCCTTTGGTT 
GAGCATATTT ATGAGCTGCA CAATATTGGA CCCAGCACCA TCAGTGACAG CATTCTTGAC 
GTGGGCTGGC CGTTCTCTGC ACGGGATGAG TTTCTCCTCT ATATTTTTCA TCTTCAAACT 
CTGGGACCTC TACAATGTCA AACAAATCCT GAGATCAACC CACAGGATAT AAAGCCTGCT 
GCCTCCCCAG AGGATACCCC TGAGCTCAGT GCCTTCTTAA GAAATGCTAC AATTCCCCAT 
CTTGTCAGAA AGAGGGATGT GCCAGTGGTC CAGCTCCACA GACAGAGCCC TGCAAGAATA 
CTGAACTGTA CCAACATTGA CTGCTTGCAA ATCTCTTGTG CAGTGGGTCG CCTGGGAGGA 
GGCGAAAGTG CAGTCCTAAA GGTCAGATCG AGATTGTGGG CCCACACGTT CCTCAAGGAA 
ATTTCTGTAG AAGAGACGAG CCAAGAGCAG TTGGTGAAGA CACTCAAGAG AAAGAATGAT 
CATTATGCTC TTGCATCCCT GGTGTCCTTT GAAGTCAAGA AGATGCCGTA TAAAGAACAG 
CCAGCAAAGC TGCCAGCGGG GAGCACAGCG GTTAAGACGT CGGTTATTTG GGCCACTCCG 
AATGTCTCCT TCTCAATTCC ATTGTGGGTC ATAATACTAG CGATCCTTCT TGGTTTGCTG 
GTTCTGGCTA TCTTAACTTT AGCTTTATGG AAGTGTGGAT TCTTTGATAG AGCAAGACCT 
CCTCAGGATG AAATGACAGA CAGGGAACAA CTGACCAGTG ACAAGACCCC AGAGGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Itga8 ( XM_006497475.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497538.1 Mus musculus integrin alpha 8 (Itga8), transcript variant X1, mRNA.

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CloneID	OMu29154
Clone ID Related Accession (Same CDS sequence)	XM_006497475.3 , XM_006497475.4
Accession Version	XM_006497475.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	integrin alpha-8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006497475.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCGG GAACCCACTG TGGTCCCCCG GGGAACCGGG CACCTCCGTT CGCGCGTCTC 
TGTTGCGTCT CGGCCGCGCT GGGGATGCTG TGGTCCCCTG CGTGTCTGGC GTTCAACTTG 
GATGTGGACA AGCTCACTGT GTACAGTGGC CCCGAGGGCA GCTACTTCGG CTACTCACTG 
GACTTCTACA TACCTGATGC GCGCACAGCC AGTGTCTTAG TGGGGGCACC CAAAGCCAAC 
ACTAGCCAGC CGGATATCGT AGAAGGGGGA GCTGTCTACT ACTGTCCCTG GCCCTCAGAG 
AGGTCTGCAC AGTGCAAGCA GATACCGTTT GACACCACCA ACAACAGGAA AATCAGAGTT 
AATGGAACCA AAGAACCTAT AGAATTTAAA TCAAACCAGT GGTTTGGAGC CACAGTGAGA 
GCTCACAAAG GAAAAGTTGT GGCTTGTGCC CCTTTGTATC ACTGGAGAAC TCTGAAACCT 
AATCCAGCGA AGGACCCAGT TGGCACATGC TATGTAGCAA TTCAGAATTT CAGTGCCTAT 
GCTGAGCACT CACCTTGTCG AAACAGCAAT GCTGATCCCG AAGGCCAAGG TTACTGCCAG 
GCAGGGTTTA GCCTAGACTT CTATAAGAAT GGAGACCTTA TAGTGGGAGG ACCTGGAAGT 
TTTTACTGGC AAGGTCAGGT GATCACTGTC AGTATAGCAG ACATCATTGC AAATTACTCA 
TTCAAGGATA TTCTACGAAA ATTGGCCGCA GAAAAGCAGA CAGACGTGGC TCCAGCTTCC 
TATGATGACA GCTACCTTGG ATATTCGGTC GCTGCTGGAG AATTCACTGG GGACTCTCAG 
CAAGAATTGG TGGCTGGGAT TCCAAGAGGA GCACAGAACT TTGGATATGT CTCCATCATT 
AACTCCACAG ACATGACCTT CATTCAGAAT TTTACTGGTG AGCAGATGGC ATCTTATTTC 
GGATATACTG TTGTGGTATC AGATGTTAAC AATGATGGCA TGGATGACAT ATTGGTCGGG 
GCACCTCTCT TTATGGAGCG AGAATTTGAA AGTAACCCTA GAGAAGTGGG ACAGGTCTAC 
TTGTACCTTC AAGCAAGCGC CCTCCTCTTC CAAGACCCAC AGGTCCTCAC CGGCACGGAG 
ACATTTGGGA GATTTGGTAG CTCTGTGGCC CACTTGGGGG ACCTGAACCA AGATGGATAC 
AATGACATCG CCATCGGTGT GCCCTTTGCA GGCAAGGATC AACGAGGTAA AGTCCTCATT 
TACAATGGAA ACCCAAGAGG TTTACACAGC AAGCCTTCCC AAGTTCTGCA AGGAATATGG 
GGGTCACAGA CCATCCCTTC TGGATTTGGC TTTTCTCTAA GAGGAGATGC AGACATAGAC 
AAGAATGATT ACCCAGATTT GCTTGTAGGG GCATTTGGAA AAGGGAAAGT AGCTGTTTAC 
AGAGCAAGGC CAGTTGTAAC CGTGGATGCC CAGCTTCTGC TGCACCCGAT GATTATCAAC 
CTTGAAAATA AAACTTGCCA GATTCCAGAA TTCCCTACTC CTGTGGCCTG CTTTTCTGTA 
AGAGTCTGTG CATCTATAGC AGGCCAGAGC ATTTCAAATA CAATAGCCCT GCTGGCCGAG 
GTGCAGTTAG ATTTCCTGAA GCAAAAAGGA GCCATCAAAC GGACGCTCTT TCTCCACAAC 
CACCAGTCCC ATTTCACCTT CCCCTTTGTG ATGAAGCAGC AGAAATCCCT CCACTGCCAG 
GATTTTATGG TTTACCTTCG GGATGAAACT GAATTCCGAG ATAAATTGTC TCCAATCAAC 
ATCAGCCTGA ACTACAGTTT GGATGATTCT ACCTTTAAAG ACAGCCTGGA AGTGAAGCCA 
ATTTTGAACC ACTACAGGGA CAATGTAGTT ACTGAGCAGG CTCACATCCT GGTGGACTGT 
GGAGAAGACA ATCTATGCGT TCCTGACTTG AAGCTGTCAG CTAGACCAGA TAAGCATCAG 
ATAATTATTG GCGATGAAAA TCACCTAATG CTCATAATAA ATGCAAGAAA TGAGGGAGAA 
GGGGCCTACG AAGCCGAACT CTTTGTTATC ATCCCCGAAG AAGCAGATTA TGTTGGGATT 
GAGCGCAACA ACAAGGGACT GAGGCCCCTG AGTTGTGAGT ACAAGATGGA AAACGTGACC 
AGGATGGTGG TGTGTGACCT TGGGAACCCG ATGGTGACTG GAACAAATTT TTCTCTGGGC 
CTCCGATTTG CTGTTCCTCG CCTTGAGAAA ACAAATATGA GCATTAACTT CGATCTCCAA 
ATCAGAAGCT CCAATAAGGA CAATCCTGAC AGCAACTTCG AGCGTGTACA GATCAACATC 
ACTGCCATTG CTCAGGTCGA AATCAGAGGA GTGTCCCACC CTCCGCAGAT TGTTCTGCCC 
ATCCACAACT GGGAACCAGA AAAGAAGCCA CACAAAGAGG AGGAAGTCGG TCCTTTGGTT 
GAGCATATTT ATGAGCTGCA CAATATTGGA CCCAGCACCA TCAGTGACAG CATTCTTGAC 
GTGGGCTGGC CGTTCTCTGC ACGGGATGAG TTTCTCCTCT ATATTTTTCA TCTTCAAACT 
CTGGGACCTC TACAATGTCA AACAAATCCT GAGATCAACC CACAGGATAT AAAGCCTGCT 
GCCTCCCCAG AGGATACCCC TGAGCTCAGT GCCTTCTTAA GAAATGCTAC AATTCCCCAT 
CTTGTCAGAA AGAGGGATGT GCCAGTGGTC CAGCTCCACA GACAGAGCCC TGCAAGAATA 
CTGAACTGTA CCAACATTGA CTGCTTGCAA ATCTCTTGTG CAGTGGGTCG CCTGGGAGGA 
GGCGAAAGTG CAGTCCTAAA GGTCAGATCG AGATTGTGGG CCCACACGTT CCTCAAGGAA 
ATTTCTGTAG AAGAGACGAG CCAAGAGCAG TTGGTGAAGA CACTCAAGAG AAAGAATGAT 
CATTATGCTC TTGCATCCCT GGTGTCCTTT GAAGTCAAGA AGATGCCGTA TAAAGAACAG 
CCAGCAAAGC TGCCAGCGGG GAGCACAGCG GTTAAGACGT CGGTTATTTG GGCCACTCCG 
AATGTCTCCT TCTCAATTCC ATTGTGGGTC ATAATACTAG CGATCCTTCT TGGTTTGCTG 
GTTCTGGCTA TCTTAACTTT AGCTTTATGG AAGTGTGGAT TCTTTGATAG AGCAAGACCT 
CCTCAGGATG AAATGACAGA CAGGGAACAA CTGACCAGTG ACAAGACCCC AGAGGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497538.1
CDS	133..3372
Translation	MSAGTHCGPPGNRAPPFARLCCVSAALGMLWSPACLAFNLDVDKLTVYSGPEGSYFGYSLDFYIPDARTASVLVGAPKANTSQPDIVEGGAVYYCPWPSERSAQCKQIPFDTTNNRKIRVNGTKEPIEFKSNQWFGATVRAHKGKVVACAPLYHWRTLKPNPAKDPVGTCYVAIQNFSAYAEHSPCRNSNADPEGQGYCQAGFSLDFYKNGDLIVGGPGSFYWQGQVITVSIADIIANYSFKDILRKLAAEKQTDVAPASYDDSYLGYSVAAGEFTGDSQQELVAGIPRGAQNFGYVSIINSTDMTFIQNFTGEQMASYFGYTVVVSDVNNDGMDDILVGAPLFMEREFESNPREVGQVYLYLQASALLFQDPQVLTGTETFGRFGSSVAHLGDLNQDGYNDIAIGVPFAGKDQRGKVLIYNGNPRGLHSKPSQVLQGIWGSQTIPSGFGFSLRGDADIDKNDYPDLLVGAFGKGKVAVYRARPVVTVDAQLLLHPMIINLENKTCQIPEFPTPVACFSVRVCASIAGQSISNTIALLAEVQLDFLKQKGAIKRTLFLHNHQSHFTFPFVMKQQKSLHCQDFMVYLRDETEFRDKLSPINISLNYSLDDSTFKDSLEVKPILNHYRDNVVTEQAHILVDCGEDNLCVPDLKLSARPDKHQIIIGDENHLMLIINARNEGEGAYEAELFVIIPEEADYVGIERNNKGLRPLSCEYKMENVTRMVVCDLGNPMVTGTNFSLGLRFAVPRLEKTNMSINFDLQIRSSNKDNPDSNFERVQINITAIAQVEIRGVSHPPQIVLPIHNWEPEKKPHKEEEVGPLVEHIYELHNIGPSTISDSILDVGWPFSARDEFLLYIFHLQTLGPLQCQTNPEINPQDIKPAASPEDTPELSAFLRNATIPHLVRKRDVPVVQLHRQSPARILNCTNIDCLQISCAVGRLGGGESAVLKVRSRLWAHTFLKEISVEETSQEQLVKTLKRKNDHYALASLVSFEVKKMPYKEQPAKLPAGSTAVKTSVIWATPNVSFSIPLWVIILAILLGLLVLAILTLALWKCGFFDRARPPQDEMTDREQLTSDKTPEA

Target ORF information:

RefSeq Version	XM_006497475.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus integrin alpha 8 (Itga8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497475.3

ORF Insert Sequence:

ATGTCTGCGG GAACCCACTG TGGTCCCCCG GGGAACCGGG CACCTCCGTT CGCGCGTCTC 
TGTTGCGTCT CGGCCGCGCT GGGGATGCTG TGGTCCCCTG CGTGTCTGGC GTTCAACTTG 
GATGTGGACA AGCTCACTGT GTACAGTGGC CCCGAGGGCA GCTACTTCGG CTACTCACTG 
GACTTCTACA TACCTGATGC GCGCACAGCC AGTGTCTTAG TGGGGGCACC CAAAGCCAAC 
ACTAGCCAGC CGGATATCGT AGAAGGGGGA GCTGTCTACT ACTGTCCCTG GCCCTCAGAG 
AGGTCTGCAC AGTGCAAGCA GATACCGTTT GACACCACCA ACAACAGGAA AATCAGAGTT 
AATGGAACCA AAGAACCTAT AGAATTTAAA TCAAACCAGT GGTTTGGAGC CACAGTGAGA 
GCTCACAAAG GAAAAGTTGT GGCTTGTGCC CCTTTGTATC ACTGGAGAAC TCTGAAACCT 
AATCCAGCGA AGGACCCAGT TGGCACATGC TATGTAGCAA TTCAGAATTT CAGTGCCTAT 
GCTGAGCACT CACCTTGTCG AAACAGCAAT GCTGATCCCG AAGGCCAAGG TTACTGCCAG 
GCAGGGTTTA GCCTAGACTT CTATAAGAAT GGAGACCTTA TAGTGGGAGG ACCTGGAAGT 
TTTTACTGGC AAGGTCAGGT GATCACTGTC AGTATAGCAG ACATCATTGC AAATTACTCA 
TTCAAGGATA TTCTACGAAA ATTGGCCGCA GAAAAGCAGA CAGACGTGGC TCCAGCTTCC 
TATGATGACA GCTACCTTGG ATATTCGGTC GCTGCTGGAG AATTCACTGG GGACTCTCAG 
CAAGAATTGG TGGCTGGGAT TCCAAGAGGA GCACAGAACT TTGGATATGT CTCCATCATT 
AACTCCACAG ACATGACCTT CATTCAGAAT TTTACTGGTG AGCAGATGGC ATCTTATTTC 
GGATATACTG TTGTGGTATC AGATGTTAAC AATGATGGCA TGGATGACAT ATTGGTCGGG 
GCACCTCTCT TTATGGAGCG AGAATTTGAA AGTAACCCTA GAGAAGTGGG ACAGGTCTAC 
TTGTACCTTC AAGCAAGCGC CCTCCTCTTC CAAGACCCAC AGGTCCTCAC CGGCACGGAG 
ACATTTGGGA GATTTGGTAG CTCTGTGGCC CACTTGGGGG ACCTGAACCA AGATGGATAC 
AATGACATCG CCATCGGTGT GCCCTTTGCA GGCAAGGATC AACGAGGTAA AGTCCTCATT 
TACAATGGAA ACCCAAGAGG TTTACACAGC AAGCCTTCCC AAGTTCTGCA AGGAATATGG 
GGGTCACAGA CCATCCCTTC TGGATTTGGC TTTTCTCTAA GAGGAGATGC AGACATAGAC 
AAGAATGATT ACCCAGATTT GCTTGTAGGG GCATTTGGAA AAGGGAAAGT AGCTGTTTAC 
AGAGCAAGGC CAGTTGTAAC CGTGGATGCC CAGCTTCTGC TGCACCCGAT GATTATCAAC 
CTTGAAAATA AAACTTGCCA GATTCCAGAA TTCCCTACTC CTGTGGCCTG CTTTTCTGTA 
AGAGTCTGTG CATCTATAGC AGGCCAGAGC ATTTCAAATA CAATAGCCCT GCTGGCCGAG 
GTGCAGTTAG ATTTCCTGAA GCAAAAAGGA GCCATCAAAC GGACGCTCTT TCTCCACAAC 
CACCAGTCCC ATTTCACCTT CCCCTTTGTG ATGAAGCAGC AGAAATCCCT CCACTGCCAG 
GATTTTATGG TTTACCTTCG GGATGAAACT GAATTCCGAG ATAAATTGTC TCCAATCAAC 
ATCAGCCTGA ACTACAGTTT GGATGATTCT ACCTTTAAAG ACAGCCTGGA AGTGAAGCCA 
ATTTTGAACC ACTACAGGGA CAATGTAGTT ACTGAGCAGG CTCACATCCT GGTGGACTGT 
GGAGAAGACA ATCTATGCGT TCCTGACTTG AAGCTGTCAG CTAGACCAGA TAAGCATCAG 
ATAATTATTG GCGATGAAAA TCACCTAATG CTCATAATAA ATGCAAGAAA TGAGGGAGAA 
GGGGCCTACG AAGCCGAACT CTTTGTTATC ATCCCCGAAG AAGCAGATTA TGTTGGGATT 
GAGCGCAACA ACAAGGGACT GAGGCCCCTG AGTTGTGAGT ACAAGATGGA AAACGTGACC 
AGGATGGTGG TGTGTGACCT TGGGAACCCG ATGGTGACTG GAACAAATTT TTCTCTGGGC 
CTCCGATTTG CTGTTCCTCG CCTTGAGAAA ACAAATATGA GCATTAACTT CGATCTCCAA 
ATCAGAAGCT CCAATAAGGA CAATCCTGAC AGCAACTTCG AGCGTGTACA GATCAACATC 
ACTGCCATTG CTCAGGTCGA AATCAGAGGA GTGTCCCACC CTCCGCAGAT TGTTCTGCCC 
ATCCACAACT GGGAACCAGA AAAGAAGCCA CACAAAGAGG AGGAAGTCGG TCCTTTGGTT 
GAGCATATTT ATGAGCTGCA CAATATTGGA CCCAGCACCA TCAGTGACAG CATTCTTGAC 
GTGGGCTGGC CGTTCTCTGC ACGGGATGAG TTTCTCCTCT ATATTTTTCA TCTTCAAACT 
CTGGGACCTC TACAATGTCA AACAAATCCT GAGATCAACC CACAGGATAT AAAGCCTGCT 
GCCTCCCCAG AGGATACCCC TGAGCTCAGT GCCTTCTTAA GAAATGCTAC AATTCCCCAT 
CTTGTCAGAA AGAGGGATGT GCCAGTGGTC CAGCTCCACA GACAGAGCCC TGCAAGAATA 
CTGAACTGTA CCAACATTGA CTGCTTGCAA ATCTCTTGTG CAGTGGGTCG CCTGGGAGGA 
GGCGAAAGTG CAGTCCTAAA GGTCAGATCG AGATTGTGGG CCCACACGTT CCTCAAGGAA 
ATTTCTGTAG AAGAGACGAG CCAAGAGCAG TTGGTGAAGA CACTCAAGAG AAAGAATGAT 
CATTATGCTC TTGCATCCCT GGTGTCCTTT GAAGTCAAGA AGATGCCGTA TAAAGAACAG 
CCAGCAAAGC TGCCAGCGGG GAGCACAGCG GTTAAGACGT CGGTTATTTG GGCCACTCCG 
AATGTCTCCT TCTCAATTCC ATTGTGGGTC ATAATACTAG CGATCCTTCT TGGTTTGCTG 
GTTCTGGCTA TCTTAACTTT AGCTTTATGG AAGTGTGGAT TCTTTGATAG AGCAAGACCT 
CCTCAGGATG AAATGACAGA CAGGGAACAA CTGACCAGTG ACAAGACCCC AGAGGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sex differences in the development of vascular and renal lesions in mice with a simultaneous deficiency of
Biology of sex differences819(2017)
Marek I,Canu M,Cordasic N,Rauh M,Volkert G,Fahlbusch FB,Rascher W,Hilgers KF,Hartner A,Menendez-Castro C

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Itga8 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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