Prkag3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Prkag3
Entrez Gene ID	241113
Full Name	protein kinase, AMP-activated, gamma 3 non-catatlytic subunit
Synonyms	AMPKg3L,AMPKg3S,Amkg3,Ampkg3
General protein information	Preferred Names protein kinase, AMP-activated, gamma 3 non-catatlytic subunit Names 5'-AMP-activated protein kinase subunit gamma-3 AMP-activated protein kinase gamma subunit AMPK gamma3 AMPK subunit gamma-3
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1\|1 C4

mRNA and Protein(s)

mRNA	Protein	Name
XM_006495988.3	XP_006496051.1	5'-AMP-activated protein kinase subunit gamma-3 isoform X1
XM_006495988.4	XP_006496051.1	5'-AMP-activated protein kinase subunit gamma-3 isoform X1
XM_006495989.3	XP_006496052.1	5'-AMP-activated protein kinase subunit gamma-3 isoform X1
NM_153744.3	NP_714966.1	5'-AMP-activated protein kinase subunit gamma-3
XM_006495989.4	XP_006496052.1	5'-AMP-activated protein kinase subunit gamma-3 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	PRKAG3	NP_059127.2
Canis lupus familiaris (dog)	PRKAG3	XP_005640723.1
Bos taurus (cattle)	PRKAG3	NP_001155892.1
Rattus norvegicus (Norway rat)	Prkag3	NP_001100391.1
Pan troglodytes (chimpanzee)	PRKAG3	XP_526030.3
	PRKAG3	XP_001091081.2
Mus musculus (house mouse)	Prkag3	NP_714966.1
Gallus gallus (chicken)	PRKAG3	NP_001026429.2
Danio rerio (zebrafish)	prkag3b	NP_001035456.1
Danio rerio (zebrafish)	prkag3a	XP_005160349.1
Xenopus tropicalis (tropical clawed frog)	prkag3	NP_001072406.2

Gene Ontology
Bibliography

Related articles in PubMed

Activation of Skeletal Muscle AMPK Promotes Glucose Disposal and Glucose Lowering in Non-human Primates and Mice.
Cokorinos EC, Delmore J, Reyes AR, Albuquerque B, Kj?bsted R, J?rgensen NO, Tran JL, Jatkar A, Cialdea K, Esquejo RM, Meissen J, Calabrese MF, Cordes J, Moccia R, Tess D, Salatto CT, Coskran TM, Opsahl AC, Flynn D, Blatnik M, Li W, Kindt E, Foretz M, Viollet B, Ward J, Kurumbail RG, Kalgutkar AS, Wojtaszewski JFP, Cameron KO, Miller RA
Cell metabolism25(5)1147-1159.e10(2017 May)

Enhanced Muscle Insulin Sensitivity After Contraction/Exercise Is Mediated by AMPK.
Kj?bsted R, Munk-Hansen N, Birk JB, Foretz M, Viollet B, Bj?rnholm M, Zierath JR, Treebak JT, Wojtaszewski JF
Diabetes66(3)598-612(2017 03)

AMPK?3 is dispensable for skeletal muscle hypertrophy induced by functional overload.
Riedl I, Osler ME, Bj?rnholm M, Egan B, Nader GA, Chibalin AV, Zierath JR
American journal of physiology. Endocrinology and metabolism310(6)E461-72(2016 Mar)

Prior AICAR stimulation increases insulin sensitivity in mouse skeletal muscle in an AMPK-dependent manner.
Kj?bsted R, Treebak JT, Fentz J, Lantier L, Viollet B, Birk JB, Schjerling P, Bj?rnholm M, Zierath JR, Wojtaszewski JF
Diabetes64(6)2042-55(2015 Jun)

Gene discovery by microarray: identification of novel genes induced during growth factor-mediated muscle cell survival and differentiation.
Kuninger D, Kuzmickas R, Peng B, Pintar JE, Rotwein P
Genomics84(5)876-89(2004 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that the AMPK-TBC1D4 signaling axis is likely mediating the improved muscle insulin sensitivity after contraction/exercise and illuminates an important and physiologically relevant role of AMPK in skeletal muscle.
Title: Enhanced Muscle Insulin Sensitivity After Contraction/Exercise Is Mediated by AMPK.

The AMPKgamma3 isoform is dispensable for functional overload-induced muscle growth.
Title: AMPK?3 is dispensable for skeletal muscle hypertrophy induced by functional overload.

gamma3-AMPK down-regulation is a rapid physiological muscle response observed in mouse after running exercise or fasting, two situations leading to PPARbeta activation.
Title: PPARbeta activation induces rapid changes of both AMPK subunit expression and AMPK activation in mouse skeletal muscle.

subunit R225Q mutation effect on growth performance with normal or high-energy diet
Title: Mouse AMP-activated protein kinase gamma3 subunit R225Q mutation affecting mouse growth performance when fed a high-energy diet.

AMPK acts as the primordial trigger for fasting- and exercise-induced adaptations in skeletal muscle and that activation of SIRT1 and its downstream signaling pathways are improperly triggered in AMPK-deficient states.
Title: Interdependence of AMPK and SIRT1 for metabolic adaptation to fasting and exercise in skeletal muscle.

The following Prkag3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prkag3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu13529	NM_153744.3 Latest version!	Mus musculus protein kinase, AMP-activated, gamma 3 non-catalytic subunit (Prkag3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu13529	XM_006495988.4 Latest version!	Mus musculus protein kinase, AMP-activated, gamma 3 non-catalytic subunit (Prkag3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu13529	XM_006495989.4 Latest version!	Mus musculus protein kinase, AMP-activated, gamma 3 non-catalytic subunit (Prkag3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OMu13529	XM_006495988.3	Mus musculus protein kinase, AMP-activated, gamma 3 non-catatlytic subunit (Prkag3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu13529	XM_006495989.3	Mus musculus protein kinase, AMP-activated, gamma 3 non-catatlytic subunit (Prkag3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prkag3 ( NM_153744.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_714966.1 Mus musculus protein kinase, AMP-activated, gamma 3 non-catalytic subunit (Prkag3), mRNA.

Price & Availability↑

CloneID	OMu13529
Clone ID Related Accession (Same CDS sequence)	XM_006495988.3 , XM_006495988.4 , XM_006495989.3 , NM_153744.3 , XM_006495989.4
Accession Version	NM_153744.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1470bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	5'-AMP-activated protein kinase subunit gamma-3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK036585.1, AC152409.8 and BX637099.1. On Jun 2, 2009 this sequence version replaced NM_153744.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK036585.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## NMD candidate :: PMID: 14512293, 14559719, 15247217 ##RefSeq-Attributes-END##

ATGGAGCCCG AGCTGGAGCA CACATTGCCT GGGACCCTGA CCTGGAGCCA CAGTGGGGGT 
CCAGAGAGTC AAGAGATGGA CTTCTTAGAA CAAGGAGAAA ACTCATGGCC CTCACCAGCT 
GTGGCCACCA GCTCAGAAAG AACCTGTGCC ATACGGGGAG TCAAGGCTTC CAGATGGACG 
AGACAGGAGG CCGTAGAGGA AGCAGAACCA CCAGGTTTGG GAGAAGGTGC CCAGTCCAGA 
CCAGCTGCTG AGTCCACCAG GCAGGAGGCC ACATTCCCGA AGGCCACACC CTTGGCTCAA 
GCTGTTCCCT TGGCTGAAGC GGAGACCTCC CCCACAGGGT GGGACCTGCT CTTGCCCGAC 
TGTGCAGCCT CAGCAGGGGG CTCCAGCACA GGTGACCTGG AGCTGACCAT AGAGTTCCCA 
GCCCCAGAGG CCTGGGACTG TGAGCTGGAA GGCCTGGGGA AGGACAGGCC TCGTCCTGGT 
CCATCCCCAC AGGCCCCACT TCTCGGCCTG AGTTGGGATG ACGAACTTCA GAAGCCCGGA 
GCCCAGGTCT ACATGCACTT CATGCAGGAA CACACCTGTT ATGATGCCAT GGCTACCAGC 
TCCAAATTGG TCATCTTTGA CACCACGTTG GAGATAAAGA AGGCTTTCTT TGCCATGGTG 
GCCAACGGTG TGAGGGCAGC TCCTCTGTGG GACAGCAAGA AGCAGAGCTT TGTGGGTATG 
CTCACCATCA CTGACTTTAT CCTGGTGCTG CACCGGTACT ACAGATCCCC CCTGGTCCAG 
ATCTATGAGA TTGAAGAACA TAAGATTGAG ACCTGGAGGG AGATCTACCT ACAAGGCTGC 
TTCAAGCCTC TAGTCTCCAT CTCTCCCAAT GACAGCCTGT TTGAAGCTGT CTATGCCCTC 
ATCAAGAACC GAATCCACCG CCTGCCGGTC CTGGACCCGG TCTCTGGCAC TGTGCTCTAC 
ATACTCACAC ACAAGCGGCT ACTCAAGTTC CTGCATATAT TTGGTGCCCT GTTGCCCCGG 
CCCTCCTTCC TCTGCCGCAC TATCCAAGAC TTGGGCATCG GCACATTCCG AGATTTGGCT 
GTAGTTCTGG AAACAGCTCC TGTCCTGACT GCGCTGGACA TCTTTGTGGA CCGACGTGTG 
TCTGCACTGC CTGTGGTCAA TGAATCTGGT CAGGTCGTGG GCCTCTACTC CCGCTTTGAT 
GTCATTCACC TGGCTGCCCA GCAAACCTAC AACCACCTAG ACATGAGTGT GGGAGAAGCT 
CTGAGACAGA GGACACTGTG TCTGGAGGGA GTTCTCTCCT GCCAGCCCCA CGAGAGCCTA 
GGTGAAGTCA TTGACAGGAT CGCACGGGAA CAGGTGCATA GGCTGGTGTT GGTGGATGAG 
ACCCAGCATC TTCTGGGCGT GGTCTCCCTC TCTGACATAC TTCAAGCACT GGTACTCAGC 
CCTGCTGGCA TCGATGCCCT CAGCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_714966.1
CDS	106..1575
Translation	MEPELEHTLPGTLTWSHSGGPESQEMDFLEQGENSWPSPAVATSSERTCAIRGVKASRWTRQEAVEEAEPPGLGEGAQSRPAAESTRQEATFPKATPLAQAVPLAEAETSPTGWDLLLPDCAASAGGSSTGDLELTIEFPAPEAWDCELEGLGKDRPRPGPSPQAPLLGLSWDDELQKPGAQVYMHFMQEHTCYDAMATSSKLVIFDTTLEIKKAFFAMVANGVRAAPLWDSKKQSFVGMLTITDFILVLHRYYRSPLVQIYEIEEHKIETWREIYLQGCFKPLVSISPNDSLFEAVYALIKNRIHRLPVLDPVSGTVLYILTHKRLLKFLHIFGALLPRPSFLCRTIQDLGIGTFRDLAVVLETAPVLTALDIFVDRRVSALPVVNESGQVVGLYSRFDVIHLAAQQTYNHLDMSVGEALRQRTLCLEGVLSCQPHESLGEVIDRIAREQVHRLVLVDETQHLLGVVSLSDILQALVLSPAGIDALSA

Target ORF information:

RefSeq Version	NM_153744.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein kinase, AMP-activated, gamma 3 non-catalytic subunit (Prkag3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153744.3

ORF Insert Sequence:

ATGGAGCCCG AGCTGGAGCA CACATTGCCT GGGACCCTGA CCTGGAGCCA CAGTGGGGGT 
CCAGAGAGTC AAGAGATGGA CTTCTTAGAA CAAGGAGAAA ACTCATGGCC CTCACCAGCT 
GTGGCCACCA GCTCAGAAAG AACCTGTGCC ATACGGGGAG TCAAGGCTTC CAGATGGACG 
AGACAGGAGG CCGTAGAGGA AGCAGAACCA CCAGGTTTGG GAGAAGGTGC CCAGTCCAGA 
CCAGCTGCTG AGTCCACCAG GCAGGAGGCC ACATTCCCGA AGGCCACACC CTTGGCTCAA 
GCTGTTCCCT TGGCTGAAGC GGAGACCTCC CCCACAGGGT GGGACCTGCT CTTGCCCGAC 
TGTGCAGCCT CAGCAGGGGG CTCCAGCACA GGTGACCTGG AGCTGACCAT AGAGTTCCCA 
GCCCCAGAGG CCTGGGACTG TGAGCTGGAA GGCCTGGGGA AGGACAGGCC TCGTCCTGGT 
CCATCCCCAC AGGCCCCACT TCTCGGCCTG AGTTGGGATG ACGAACTTCA GAAGCCCGGA 
GCCCAGGTCT ACATGCACTT CATGCAGGAA CACACCTGTT ATGATGCCAT GGCTACCAGC 
TCCAAATTGG TCATCTTTGA CACCACGTTG GAGATAAAGA AGGCTTTCTT TGCCATGGTG 
GCCAACGGTG TGAGGGCAGC TCCTCTGTGG GACAGCAAGA AGCAGAGCTT TGTGGGTATG 
CTCACCATCA CTGACTTTAT CCTGGTGCTG CACCGGTACT ACAGATCCCC CCTGGTCCAG 
ATCTATGAGA TTGAAGAACA TAAGATTGAG ACCTGGAGGG AGATCTACCT ACAAGGCTGC 
TTCAAGCCTC TAGTCTCCAT CTCTCCCAAT GACAGCCTGT TTGAAGCTGT CTATGCCCTC 
ATCAAGAACC GAATCCACCG CCTGCCGGTC CTGGACCCGG TCTCTGGCAC TGTGCTCTAC 
ATACTCACAC ACAAGCGGCT ACTCAAGTTC CTGCATATAT TTGGTGCCCT GTTGCCCCGG 
CCCTCCTTCC TCTGCCGCAC TATCCAAGAC TTGGGCATCG GCACATTCCG AGATTTGGCT 
GTAGTTCTGG AAACAGCTCC TGTCCTGACT GCGCTGGACA TCTTTGTGGA CCGACGTGTG 
TCTGCACTGC CTGTGGTCAA TGAATCTGGT CAGGTCGTGG GCCTCTACTC CCGCTTTGAT 
GTCATTCACC TGGCTGCCCA GCAAACCTAC AACCACCTAG ACATGAGTGT GGGAGAAGCT 
CTGAGACAGA GGACACTGTG TCTGGAGGGA GTTCTCTCCT GCCAGCCCCA CGAGAGCCTA 
GGTGAAGTCA TTGACAGGAT CGCACGGGAA CAGGTGCATA GGCTGGTGTT GGTGGATGAG 
ACCCAGCATC TTCTGGGCGT GGTCTCCCTC TCTGACATAC TTCAAGCACT GGTACTCAGC 
CCTGCTGGCA TCGATGCCCT CAGCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prkag3 ( XM_006495988.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496051.1 Mus musculus protein kinase, AMP-activated, gamma 3 non-catatlytic subunit (Prkag3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu13529
Clone ID Related Accession (Same CDS sequence)	XM_006495988.3 , XM_006495988.4 , XM_006495989.3 , NM_153744.3 , XM_006495989.4
Accession Version	XM_006495988.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1470bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	5'-AMP-activated protein kinase subunit gamma-3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006495988.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCCG AGCTGGAGCA CACATTGCCT GGGACCCTGA CCTGGAGCCA CAGTGGGGGT 
CCAGAGAGTC AAGAGATGGA CTTCTTAGAA CAAGGAGAAA ACTCATGGCC CTCACCAGCT 
GTGGCCACCA GCTCAGAAAG AACCTGTGCC ATACGGGGAG TCAAGGCTTC CAGATGGACG 
AGACAGGAGG CCGTAGAGGA AGCAGAACCA CCAGGTTTGG GAGAAGGTGC CCAGTCCAGA 
CCAGCTGCTG AGTCCACCAG GCAGGAGGCC ACATTCCCGA AGGCCACACC CTTGGCTCAA 
GCTGTTCCCT TGGCTGAAGC GGAGACCTCC CCCACAGGGT GGGACCTGCT CTTGCCCGAC 
TGTGCAGCCT CAGCAGGGGG CTCCAGCACA GGTGACCTGG AGCTGACCAT AGAGTTCCCA 
GCCCCAGAGG CCTGGGACTG TGAGCTGGAA GGCCTGGGGA AGGACAGGCC TCGTCCTGGT 
CCATCCCCAC AGGCCCCACT TCTCGGCCTG AGTTGGGATG ACGAACTTCA GAAGCCCGGA 
GCCCAGGTCT ACATGCACTT CATGCAGGAA CACACCTGTT ATGATGCCAT GGCTACCAGC 
TCCAAATTGG TCATCTTTGA CACCACGTTG GAGATAAAGA AGGCTTTCTT TGCCATGGTG 
GCCAACGGTG TGAGGGCAGC TCCTCTGTGG GACAGCAAGA AGCAGAGCTT TGTGGGTATG 
CTCACCATCA CTGACTTTAT CCTGGTGCTG CACCGGTACT ACAGATCCCC CCTGGTCCAG 
ATCTATGAGA TTGAAGAACA TAAGATTGAG ACCTGGAGGG AGATCTACCT ACAAGGCTGC 
TTCAAGCCTC TAGTCTCCAT CTCTCCCAAT GACAGCCTGT TTGAAGCTGT CTATGCCCTC 
ATCAAGAACC GAATCCACCG CCTGCCGGTC CTGGACCCGG TCTCTGGCAC TGTGCTCTAC 
ATACTCACAC ACAAGCGGCT ACTCAAGTTC CTGCATATAT TTGGTGCCCT GTTGCCCCGG 
CCCTCCTTCC TCTGCCGCAC TATCCAAGAC TTGGGCATCG GCACATTCCG AGATTTGGCT 
GTAGTTCTGG AAACAGCTCC TGTCCTGACT GCGCTGGACA TCTTTGTGGA CCGACGTGTG 
TCTGCACTGC CTGTGGTCAA TGAATCTGGT CAGGTCGTGG GCCTCTACTC CCGCTTTGAT 
GTCATTCACC TGGCTGCCCA GCAAACCTAC AACCACCTAG ACATGAGTGT GGGAGAAGCT 
CTGAGACAGA GGACACTGTG TCTGGAGGGA GTTCTCTCCT GCCAGCCCCA CGAGAGCCTA 
GGTGAAGTCA TTGACAGGAT CGCACGGGAA CAGGTGCATA GGCTGGTGTT GGTGGATGAG 
ACCCAGCATC TTCTGGGCGT GGTCTCCCTC TCTGACATAC TTCAAGCACT GGTACTCAGC 
CCTGCTGGCA TCGATGCCCT CAGCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496051.1
CDS	429..1898
Translation	MEPELEHTLPGTLTWSHSGGPESQEMDFLEQGENSWPSPAVATSSERTCAIRGVKASRWTRQEAVEEAEPPGLGEGAQSRPAAESTRQEATFPKATPLAQAVPLAEAETSPTGWDLLLPDCAASAGGSSTGDLELTIEFPAPEAWDCELEGLGKDRPRPGPSPQAPLLGLSWDDELQKPGAQVYMHFMQEHTCYDAMATSSKLVIFDTTLEIKKAFFAMVANGVRAAPLWDSKKQSFVGMLTITDFILVLHRYYRSPLVQIYEIEEHKIETWREIYLQGCFKPLVSISPNDSLFEAVYALIKNRIHRLPVLDPVSGTVLYILTHKRLLKFLHIFGALLPRPSFLCRTIQDLGIGTFRDLAVVLETAPVLTALDIFVDRRVSALPVVNESGQVVGLYSRFDVIHLAAQQTYNHLDMSVGEALRQRTLCLEGVLSCQPHESLGEVIDRIAREQVHRLVLVDETQHLLGVVSLSDILQALVLSPAGIDALSA

Target ORF information:

RefSeq Version	XM_006495988.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein kinase, AMP-activated, gamma 3 non-catatlytic subunit (Prkag3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006495988.3

ORF Insert Sequence:

ATGGAGCCCG AGCTGGAGCA CACATTGCCT GGGACCCTGA CCTGGAGCCA CAGTGGGGGT 
CCAGAGAGTC AAGAGATGGA CTTCTTAGAA CAAGGAGAAA ACTCATGGCC CTCACCAGCT 
GTGGCCACCA GCTCAGAAAG AACCTGTGCC ATACGGGGAG TCAAGGCTTC CAGATGGACG 
AGACAGGAGG CCGTAGAGGA AGCAGAACCA CCAGGTTTGG GAGAAGGTGC CCAGTCCAGA 
CCAGCTGCTG AGTCCACCAG GCAGGAGGCC ACATTCCCGA AGGCCACACC CTTGGCTCAA 
GCTGTTCCCT TGGCTGAAGC GGAGACCTCC CCCACAGGGT GGGACCTGCT CTTGCCCGAC 
TGTGCAGCCT CAGCAGGGGG CTCCAGCACA GGTGACCTGG AGCTGACCAT AGAGTTCCCA 
GCCCCAGAGG CCTGGGACTG TGAGCTGGAA GGCCTGGGGA AGGACAGGCC TCGTCCTGGT 
CCATCCCCAC AGGCCCCACT TCTCGGCCTG AGTTGGGATG ACGAACTTCA GAAGCCCGGA 
GCCCAGGTCT ACATGCACTT CATGCAGGAA CACACCTGTT ATGATGCCAT GGCTACCAGC 
TCCAAATTGG TCATCTTTGA CACCACGTTG GAGATAAAGA AGGCTTTCTT TGCCATGGTG 
GCCAACGGTG TGAGGGCAGC TCCTCTGTGG GACAGCAAGA AGCAGAGCTT TGTGGGTATG 
CTCACCATCA CTGACTTTAT CCTGGTGCTG CACCGGTACT ACAGATCCCC CCTGGTCCAG 
ATCTATGAGA TTGAAGAACA TAAGATTGAG ACCTGGAGGG AGATCTACCT ACAAGGCTGC 
TTCAAGCCTC TAGTCTCCAT CTCTCCCAAT GACAGCCTGT TTGAAGCTGT CTATGCCCTC 
ATCAAGAACC GAATCCACCG CCTGCCGGTC CTGGACCCGG TCTCTGGCAC TGTGCTCTAC 
ATACTCACAC ACAAGCGGCT ACTCAAGTTC CTGCATATAT TTGGTGCCCT GTTGCCCCGG 
CCCTCCTTCC TCTGCCGCAC TATCCAAGAC TTGGGCATCG GCACATTCCG AGATTTGGCT 
GTAGTTCTGG AAACAGCTCC TGTCCTGACT GCGCTGGACA TCTTTGTGGA CCGACGTGTG 
TCTGCACTGC CTGTGGTCAA TGAATCTGGT CAGGTCGTGG GCCTCTACTC CCGCTTTGAT 
GTCATTCACC TGGCTGCCCA GCAAACCTAC AACCACCTAG ACATGAGTGT GGGAGAAGCT 
CTGAGACAGA GGACACTGTG TCTGGAGGGA GTTCTCTCCT GCCAGCCCCA CGAGAGCCTA 
GGTGAAGTCA TTGACAGGAT CGCACGGGAA CAGGTGCATA GGCTGGTGTT GGTGGATGAG 
ACCCAGCATC TTCTGGGCGT GGTCTCCCTC TCTGACATAC TTCAAGCACT GGTACTCAGC 
CCTGCTGGCA TCGATGCCCT CAGCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prkag3 ( XM_006495989.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496052.1 Mus musculus protein kinase, AMP-activated, gamma 3 non-catatlytic subunit (Prkag3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu13529
Clone ID Related Accession (Same CDS sequence)	XM_006495988.3 , XM_006495988.4 , XM_006495989.3 , NM_153744.3 , XM_006495989.4
Accession Version	XM_006495989.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1470bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	5'-AMP-activated protein kinase subunit gamma-3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006495989.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCCG AGCTGGAGCA CACATTGCCT GGGACCCTGA CCTGGAGCCA CAGTGGGGGT 
CCAGAGAGTC AAGAGATGGA CTTCTTAGAA CAAGGAGAAA ACTCATGGCC CTCACCAGCT 
GTGGCCACCA GCTCAGAAAG AACCTGTGCC ATACGGGGAG TCAAGGCTTC CAGATGGACG 
AGACAGGAGG CCGTAGAGGA AGCAGAACCA CCAGGTTTGG GAGAAGGTGC CCAGTCCAGA 
CCAGCTGCTG AGTCCACCAG GCAGGAGGCC ACATTCCCGA AGGCCACACC CTTGGCTCAA 
GCTGTTCCCT TGGCTGAAGC GGAGACCTCC CCCACAGGGT GGGACCTGCT CTTGCCCGAC 
TGTGCAGCCT CAGCAGGGGG CTCCAGCACA GGTGACCTGG AGCTGACCAT AGAGTTCCCA 
GCCCCAGAGG CCTGGGACTG TGAGCTGGAA GGCCTGGGGA AGGACAGGCC TCGTCCTGGT 
CCATCCCCAC AGGCCCCACT TCTCGGCCTG AGTTGGGATG ACGAACTTCA GAAGCCCGGA 
GCCCAGGTCT ACATGCACTT CATGCAGGAA CACACCTGTT ATGATGCCAT GGCTACCAGC 
TCCAAATTGG TCATCTTTGA CACCACGTTG GAGATAAAGA AGGCTTTCTT TGCCATGGTG 
GCCAACGGTG TGAGGGCAGC TCCTCTGTGG GACAGCAAGA AGCAGAGCTT TGTGGGTATG 
CTCACCATCA CTGACTTTAT CCTGGTGCTG CACCGGTACT ACAGATCCCC CCTGGTCCAG 
ATCTATGAGA TTGAAGAACA TAAGATTGAG ACCTGGAGGG AGATCTACCT ACAAGGCTGC 
TTCAAGCCTC TAGTCTCCAT CTCTCCCAAT GACAGCCTGT TTGAAGCTGT CTATGCCCTC 
ATCAAGAACC GAATCCACCG CCTGCCGGTC CTGGACCCGG TCTCTGGCAC TGTGCTCTAC 
ATACTCACAC ACAAGCGGCT ACTCAAGTTC CTGCATATAT TTGGTGCCCT GTTGCCCCGG 
CCCTCCTTCC TCTGCCGCAC TATCCAAGAC TTGGGCATCG GCACATTCCG AGATTTGGCT 
GTAGTTCTGG AAACAGCTCC TGTCCTGACT GCGCTGGACA TCTTTGTGGA CCGACGTGTG 
TCTGCACTGC CTGTGGTCAA TGAATCTGGT CAGGTCGTGG GCCTCTACTC CCGCTTTGAT 
GTCATTCACC TGGCTGCCCA GCAAACCTAC AACCACCTAG ACATGAGTGT GGGAGAAGCT 
CTGAGACAGA GGACACTGTG TCTGGAGGGA GTTCTCTCCT GCCAGCCCCA CGAGAGCCTA 
GGTGAAGTCA TTGACAGGAT CGCACGGGAA CAGGTGCATA GGCTGGTGTT GGTGGATGAG 
ACCCAGCATC TTCTGGGCGT GGTCTCCCTC TCTGACATAC TTCAAGCACT GGTACTCAGC 
CCTGCTGGCA TCGATGCCCT CAGCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496052.1
CDS	419..1888
Translation	MEPELEHTLPGTLTWSHSGGPESQEMDFLEQGENSWPSPAVATSSERTCAIRGVKASRWTRQEAVEEAEPPGLGEGAQSRPAAESTRQEATFPKATPLAQAVPLAEAETSPTGWDLLLPDCAASAGGSSTGDLELTIEFPAPEAWDCELEGLGKDRPRPGPSPQAPLLGLSWDDELQKPGAQVYMHFMQEHTCYDAMATSSKLVIFDTTLEIKKAFFAMVANGVRAAPLWDSKKQSFVGMLTITDFILVLHRYYRSPLVQIYEIEEHKIETWREIYLQGCFKPLVSISPNDSLFEAVYALIKNRIHRLPVLDPVSGTVLYILTHKRLLKFLHIFGALLPRPSFLCRTIQDLGIGTFRDLAVVLETAPVLTALDIFVDRRVSALPVVNESGQVVGLYSRFDVIHLAAQQTYNHLDMSVGEALRQRTLCLEGVLSCQPHESLGEVIDRIAREQVHRLVLVDETQHLLGVVSLSDILQALVLSPAGIDALSA

Target ORF information:

RefSeq Version	XM_006495989.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein kinase, AMP-activated, gamma 3 non-catatlytic subunit (Prkag3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006495989.3

ORF Insert Sequence:

ATGGAGCCCG AGCTGGAGCA CACATTGCCT GGGACCCTGA CCTGGAGCCA CAGTGGGGGT 
CCAGAGAGTC AAGAGATGGA CTTCTTAGAA CAAGGAGAAA ACTCATGGCC CTCACCAGCT 
GTGGCCACCA GCTCAGAAAG AACCTGTGCC ATACGGGGAG TCAAGGCTTC CAGATGGACG 
AGACAGGAGG CCGTAGAGGA AGCAGAACCA CCAGGTTTGG GAGAAGGTGC CCAGTCCAGA 
CCAGCTGCTG AGTCCACCAG GCAGGAGGCC ACATTCCCGA AGGCCACACC CTTGGCTCAA 
GCTGTTCCCT TGGCTGAAGC GGAGACCTCC CCCACAGGGT GGGACCTGCT CTTGCCCGAC 
TGTGCAGCCT CAGCAGGGGG CTCCAGCACA GGTGACCTGG AGCTGACCAT AGAGTTCCCA 
GCCCCAGAGG CCTGGGACTG TGAGCTGGAA GGCCTGGGGA AGGACAGGCC TCGTCCTGGT 
CCATCCCCAC AGGCCCCACT TCTCGGCCTG AGTTGGGATG ACGAACTTCA GAAGCCCGGA 
GCCCAGGTCT ACATGCACTT CATGCAGGAA CACACCTGTT ATGATGCCAT GGCTACCAGC 
TCCAAATTGG TCATCTTTGA CACCACGTTG GAGATAAAGA AGGCTTTCTT TGCCATGGTG 
GCCAACGGTG TGAGGGCAGC TCCTCTGTGG GACAGCAAGA AGCAGAGCTT TGTGGGTATG 
CTCACCATCA CTGACTTTAT CCTGGTGCTG CACCGGTACT ACAGATCCCC CCTGGTCCAG 
ATCTATGAGA TTGAAGAACA TAAGATTGAG ACCTGGAGGG AGATCTACCT ACAAGGCTGC 
TTCAAGCCTC TAGTCTCCAT CTCTCCCAAT GACAGCCTGT TTGAAGCTGT CTATGCCCTC 
ATCAAGAACC GAATCCACCG CCTGCCGGTC CTGGACCCGG TCTCTGGCAC TGTGCTCTAC 
ATACTCACAC ACAAGCGGCT ACTCAAGTTC CTGCATATAT TTGGTGCCCT GTTGCCCCGG 
CCCTCCTTCC TCTGCCGCAC TATCCAAGAC TTGGGCATCG GCACATTCCG AGATTTGGCT 
GTAGTTCTGG AAACAGCTCC TGTCCTGACT GCGCTGGACA TCTTTGTGGA CCGACGTGTG 
TCTGCACTGC CTGTGGTCAA TGAATCTGGT CAGGTCGTGG GCCTCTACTC CCGCTTTGAT 
GTCATTCACC TGGCTGCCCA GCAAACCTAC AACCACCTAG ACATGAGTGT GGGAGAAGCT 
CTGAGACAGA GGACACTGTG TCTGGAGGGA GTTCTCTCCT GCCAGCCCCA CGAGAGCCTA 
GGTGAAGTCA TTGACAGGAT CGCACGGGAA CAGGTGCATA GGCTGGTGTT GGTGGATGAG 
ACCCAGCATC TTCTGGGCGT GGTCTCCCTC TCTGACATAC TTCAAGCACT GGTACTCAGC 
CCTGCTGGCA TCGATGCCCT CAGCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prkag3 ( XM_006495988.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496051.1 Mus musculus protein kinase, AMP-activated, gamma 3 non-catalytic subunit (Prkag3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu13529
Clone ID Related Accession (Same CDS sequence)	XM_006495988.3 , XM_006495988.4 , XM_006495989.3 , NM_153744.3 , XM_006495989.4
Accession Version	XM_006495988.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1470bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	5'-AMP-activated protein kinase subunit gamma-3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006495988.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCCG AGCTGGAGCA CACATTGCCT GGGACCCTGA CCTGGAGCCA CAGTGGGGGT 
CCAGAGAGTC AAGAGATGGA CTTCTTAGAA CAAGGAGAAA ACTCATGGCC CTCACCAGCT 
GTGGCCACCA GCTCAGAAAG AACCTGTGCC ATACGGGGAG TCAAGGCTTC CAGATGGACG 
AGACAGGAGG CCGTAGAGGA AGCAGAACCA CCAGGTTTGG GAGAAGGTGC CCAGTCCAGA 
CCAGCTGCTG AGTCCACCAG GCAGGAGGCC ACATTCCCGA AGGCCACACC CTTGGCTCAA 
GCTGTTCCCT TGGCTGAAGC GGAGACCTCC CCCACAGGGT GGGACCTGCT CTTGCCCGAC 
TGTGCAGCCT CAGCAGGGGG CTCCAGCACA GGTGACCTGG AGCTGACCAT AGAGTTCCCA 
GCCCCAGAGG CCTGGGACTG TGAGCTGGAA GGCCTGGGGA AGGACAGGCC TCGTCCTGGT 
CCATCCCCAC AGGCCCCACT TCTCGGCCTG AGTTGGGATG ACGAACTTCA GAAGCCCGGA 
GCCCAGGTCT ACATGCACTT CATGCAGGAA CACACCTGTT ATGATGCCAT GGCTACCAGC 
TCCAAATTGG TCATCTTTGA CACCACGTTG GAGATAAAGA AGGCTTTCTT TGCCATGGTG 
GCCAACGGTG TGAGGGCAGC TCCTCTGTGG GACAGCAAGA AGCAGAGCTT TGTGGGTATG 
CTCACCATCA CTGACTTTAT CCTGGTGCTG CACCGGTACT ACAGATCCCC CCTGGTCCAG 
ATCTATGAGA TTGAAGAACA TAAGATTGAG ACCTGGAGGG AGATCTACCT ACAAGGCTGC 
TTCAAGCCTC TAGTCTCCAT CTCTCCCAAT GACAGCCTGT TTGAAGCTGT CTATGCCCTC 
ATCAAGAACC GAATCCACCG CCTGCCGGTC CTGGACCCGG TCTCTGGCAC TGTGCTCTAC 
ATACTCACAC ACAAGCGGCT ACTCAAGTTC CTGCATATAT TTGGTGCCCT GTTGCCCCGG 
CCCTCCTTCC TCTGCCGCAC TATCCAAGAC TTGGGCATCG GCACATTCCG AGATTTGGCT 
GTAGTTCTGG AAACAGCTCC TGTCCTGACT GCGCTGGACA TCTTTGTGGA CCGACGTGTG 
TCTGCACTGC CTGTGGTCAA TGAATCTGGT CAGGTCGTGG GCCTCTACTC CCGCTTTGAT 
GTCATTCACC TGGCTGCCCA GCAAACCTAC AACCACCTAG ACATGAGTGT GGGAGAAGCT 
CTGAGACAGA GGACACTGTG TCTGGAGGGA GTTCTCTCCT GCCAGCCCCA CGAGAGCCTA 
GGTGAAGTCA TTGACAGGAT CGCACGGGAA CAGGTGCATA GGCTGGTGTT GGTGGATGAG 
ACCCAGCATC TTCTGGGCGT GGTCTCCCTC TCTGACATAC TTCAAGCACT GGTACTCAGC 
CCTGCTGGCA TCGATGCCCT CAGCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496051.1
CDS	308..1777
Translation	MEPELEHTLPGTLTWSHSGGPESQEMDFLEQGENSWPSPAVATSSERTCAIRGVKASRWTRQEAVEEAEPPGLGEGAQSRPAAESTRQEATFPKATPLAQAVPLAEAETSPTGWDLLLPDCAASAGGSSTGDLELTIEFPAPEAWDCELEGLGKDRPRPGPSPQAPLLGLSWDDELQKPGAQVYMHFMQEHTCYDAMATSSKLVIFDTTLEIKKAFFAMVANGVRAAPLWDSKKQSFVGMLTITDFILVLHRYYRSPLVQIYEIEEHKIETWREIYLQGCFKPLVSISPNDSLFEAVYALIKNRIHRLPVLDPVSGTVLYILTHKRLLKFLHIFGALLPRPSFLCRTIQDLGIGTFRDLAVVLETAPVLTALDIFVDRRVSALPVVNESGQVVGLYSRFDVIHLAAQQTYNHLDMSVGEALRQRTLCLEGVLSCQPHESLGEVIDRIAREQVHRLVLVDETQHLLGVVSLSDILQALVLSPAGIDALSA

Target ORF information:

RefSeq Version	XM_006495988.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein kinase, AMP-activated, gamma 3 non-catalytic subunit (Prkag3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006495988.4

ORF Insert Sequence:

ATGGAGCCCG AGCTGGAGCA CACATTGCCT GGGACCCTGA CCTGGAGCCA CAGTGGGGGT 
CCAGAGAGTC AAGAGATGGA CTTCTTAGAA CAAGGAGAAA ACTCATGGCC CTCACCAGCT 
GTGGCCACCA GCTCAGAAAG AACCTGTGCC ATACGGGGAG TCAAGGCTTC CAGATGGACG 
AGACAGGAGG CCGTAGAGGA AGCAGAACCA CCAGGTTTGG GAGAAGGTGC CCAGTCCAGA 
CCAGCTGCTG AGTCCACCAG GCAGGAGGCC ACATTCCCGA AGGCCACACC CTTGGCTCAA 
GCTGTTCCCT TGGCTGAAGC GGAGACCTCC CCCACAGGGT GGGACCTGCT CTTGCCCGAC 
TGTGCAGCCT CAGCAGGGGG CTCCAGCACA GGTGACCTGG AGCTGACCAT AGAGTTCCCA 
GCCCCAGAGG CCTGGGACTG TGAGCTGGAA GGCCTGGGGA AGGACAGGCC TCGTCCTGGT 
CCATCCCCAC AGGCCCCACT TCTCGGCCTG AGTTGGGATG ACGAACTTCA GAAGCCCGGA 
GCCCAGGTCT ACATGCACTT CATGCAGGAA CACACCTGTT ATGATGCCAT GGCTACCAGC 
TCCAAATTGG TCATCTTTGA CACCACGTTG GAGATAAAGA AGGCTTTCTT TGCCATGGTG 
GCCAACGGTG TGAGGGCAGC TCCTCTGTGG GACAGCAAGA AGCAGAGCTT TGTGGGTATG 
CTCACCATCA CTGACTTTAT CCTGGTGCTG CACCGGTACT ACAGATCCCC CCTGGTCCAG 
ATCTATGAGA TTGAAGAACA TAAGATTGAG ACCTGGAGGG AGATCTACCT ACAAGGCTGC 
TTCAAGCCTC TAGTCTCCAT CTCTCCCAAT GACAGCCTGT TTGAAGCTGT CTATGCCCTC 
ATCAAGAACC GAATCCACCG CCTGCCGGTC CTGGACCCGG TCTCTGGCAC TGTGCTCTAC 
ATACTCACAC ACAAGCGGCT ACTCAAGTTC CTGCATATAT TTGGTGCCCT GTTGCCCCGG 
CCCTCCTTCC TCTGCCGCAC TATCCAAGAC TTGGGCATCG GCACATTCCG AGATTTGGCT 
GTAGTTCTGG AAACAGCTCC TGTCCTGACT GCGCTGGACA TCTTTGTGGA CCGACGTGTG 
TCTGCACTGC CTGTGGTCAA TGAATCTGGT CAGGTCGTGG GCCTCTACTC CCGCTTTGAT 
GTCATTCACC TGGCTGCCCA GCAAACCTAC AACCACCTAG ACATGAGTGT GGGAGAAGCT 
CTGAGACAGA GGACACTGTG TCTGGAGGGA GTTCTCTCCT GCCAGCCCCA CGAGAGCCTA 
GGTGAAGTCA TTGACAGGAT CGCACGGGAA CAGGTGCATA GGCTGGTGTT GGTGGATGAG 
ACCCAGCATC TTCTGGGCGT GGTCTCCCTC TCTGACATAC TTCAAGCACT GGTACTCAGC 
CCTGCTGGCA TCGATGCCCT CAGCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prkag3 ( XM_006495989.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496052.1 Mus musculus protein kinase, AMP-activated, gamma 3 non-catalytic subunit (Prkag3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu13529
Clone ID Related Accession (Same CDS sequence)	XM_006495988.3 , XM_006495988.4 , XM_006495989.3 , NM_153744.3 , XM_006495989.4
Accession Version	XM_006495989.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1470bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	5'-AMP-activated protein kinase subunit gamma-3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006495989.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCCG AGCTGGAGCA CACATTGCCT GGGACCCTGA CCTGGAGCCA CAGTGGGGGT 
CCAGAGAGTC AAGAGATGGA CTTCTTAGAA CAAGGAGAAA ACTCATGGCC CTCACCAGCT 
GTGGCCACCA GCTCAGAAAG AACCTGTGCC ATACGGGGAG TCAAGGCTTC CAGATGGACG 
AGACAGGAGG CCGTAGAGGA AGCAGAACCA CCAGGTTTGG GAGAAGGTGC CCAGTCCAGA 
CCAGCTGCTG AGTCCACCAG GCAGGAGGCC ACATTCCCGA AGGCCACACC CTTGGCTCAA 
GCTGTTCCCT TGGCTGAAGC GGAGACCTCC CCCACAGGGT GGGACCTGCT CTTGCCCGAC 
TGTGCAGCCT CAGCAGGGGG CTCCAGCACA GGTGACCTGG AGCTGACCAT AGAGTTCCCA 
GCCCCAGAGG CCTGGGACTG TGAGCTGGAA GGCCTGGGGA AGGACAGGCC TCGTCCTGGT 
CCATCCCCAC AGGCCCCACT TCTCGGCCTG AGTTGGGATG ACGAACTTCA GAAGCCCGGA 
GCCCAGGTCT ACATGCACTT CATGCAGGAA CACACCTGTT ATGATGCCAT GGCTACCAGC 
TCCAAATTGG TCATCTTTGA CACCACGTTG GAGATAAAGA AGGCTTTCTT TGCCATGGTG 
GCCAACGGTG TGAGGGCAGC TCCTCTGTGG GACAGCAAGA AGCAGAGCTT TGTGGGTATG 
CTCACCATCA CTGACTTTAT CCTGGTGCTG CACCGGTACT ACAGATCCCC CCTGGTCCAG 
ATCTATGAGA TTGAAGAACA TAAGATTGAG ACCTGGAGGG AGATCTACCT ACAAGGCTGC 
TTCAAGCCTC TAGTCTCCAT CTCTCCCAAT GACAGCCTGT TTGAAGCTGT CTATGCCCTC 
ATCAAGAACC GAATCCACCG CCTGCCGGTC CTGGACCCGG TCTCTGGCAC TGTGCTCTAC 
ATACTCACAC ACAAGCGGCT ACTCAAGTTC CTGCATATAT TTGGTGCCCT GTTGCCCCGG 
CCCTCCTTCC TCTGCCGCAC TATCCAAGAC TTGGGCATCG GCACATTCCG AGATTTGGCT 
GTAGTTCTGG AAACAGCTCC TGTCCTGACT GCGCTGGACA TCTTTGTGGA CCGACGTGTG 
TCTGCACTGC CTGTGGTCAA TGAATCTGGT CAGGTCGTGG GCCTCTACTC CCGCTTTGAT 
GTCATTCACC TGGCTGCCCA GCAAACCTAC AACCACCTAG ACATGAGTGT GGGAGAAGCT 
CTGAGACAGA GGACACTGTG TCTGGAGGGA GTTCTCTCCT GCCAGCCCCA CGAGAGCCTA 
GGTGAAGTCA TTGACAGGAT CGCACGGGAA CAGGTGCATA GGCTGGTGTT GGTGGATGAG 
ACCCAGCATC TTCTGGGCGT GGTCTCCCTC TCTGACATAC TTCAAGCACT GGTACTCAGC 
CCTGCTGGCA TCGATGCCCT CAGCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496052.1
CDS	288..1757
Translation	MEPELEHTLPGTLTWSHSGGPESQEMDFLEQGENSWPSPAVATSSERTCAIRGVKASRWTRQEAVEEAEPPGLGEGAQSRPAAESTRQEATFPKATPLAQAVPLAEAETSPTGWDLLLPDCAASAGGSSTGDLELTIEFPAPEAWDCELEGLGKDRPRPGPSPQAPLLGLSWDDELQKPGAQVYMHFMQEHTCYDAMATSSKLVIFDTTLEIKKAFFAMVANGVRAAPLWDSKKQSFVGMLTITDFILVLHRYYRSPLVQIYEIEEHKIETWREIYLQGCFKPLVSISPNDSLFEAVYALIKNRIHRLPVLDPVSGTVLYILTHKRLLKFLHIFGALLPRPSFLCRTIQDLGIGTFRDLAVVLETAPVLTALDIFVDRRVSALPVVNESGQVVGLYSRFDVIHLAAQQTYNHLDMSVGEALRQRTLCLEGVLSCQPHESLGEVIDRIAREQVHRLVLVDETQHLLGVVSLSDILQALVLSPAGIDALSA

Target ORF information:

RefSeq Version	XM_006495989.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein kinase, AMP-activated, gamma 3 non-catalytic subunit (Prkag3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006495989.4

ORF Insert Sequence:

ATGGAGCCCG AGCTGGAGCA CACATTGCCT GGGACCCTGA CCTGGAGCCA CAGTGGGGGT 
CCAGAGAGTC AAGAGATGGA CTTCTTAGAA CAAGGAGAAA ACTCATGGCC CTCACCAGCT 
GTGGCCACCA GCTCAGAAAG AACCTGTGCC ATACGGGGAG TCAAGGCTTC CAGATGGACG 
AGACAGGAGG CCGTAGAGGA AGCAGAACCA CCAGGTTTGG GAGAAGGTGC CCAGTCCAGA 
CCAGCTGCTG AGTCCACCAG GCAGGAGGCC ACATTCCCGA AGGCCACACC CTTGGCTCAA 
GCTGTTCCCT TGGCTGAAGC GGAGACCTCC CCCACAGGGT GGGACCTGCT CTTGCCCGAC 
TGTGCAGCCT CAGCAGGGGG CTCCAGCACA GGTGACCTGG AGCTGACCAT AGAGTTCCCA 
GCCCCAGAGG CCTGGGACTG TGAGCTGGAA GGCCTGGGGA AGGACAGGCC TCGTCCTGGT 
CCATCCCCAC AGGCCCCACT TCTCGGCCTG AGTTGGGATG ACGAACTTCA GAAGCCCGGA 
GCCCAGGTCT ACATGCACTT CATGCAGGAA CACACCTGTT ATGATGCCAT GGCTACCAGC 
TCCAAATTGG TCATCTTTGA CACCACGTTG GAGATAAAGA AGGCTTTCTT TGCCATGGTG 
GCCAACGGTG TGAGGGCAGC TCCTCTGTGG GACAGCAAGA AGCAGAGCTT TGTGGGTATG 
CTCACCATCA CTGACTTTAT CCTGGTGCTG CACCGGTACT ACAGATCCCC CCTGGTCCAG 
ATCTATGAGA TTGAAGAACA TAAGATTGAG ACCTGGAGGG AGATCTACCT ACAAGGCTGC 
TTCAAGCCTC TAGTCTCCAT CTCTCCCAAT GACAGCCTGT TTGAAGCTGT CTATGCCCTC 
ATCAAGAACC GAATCCACCG CCTGCCGGTC CTGGACCCGG TCTCTGGCAC TGTGCTCTAC 
ATACTCACAC ACAAGCGGCT ACTCAAGTTC CTGCATATAT TTGGTGCCCT GTTGCCCCGG 
CCCTCCTTCC TCTGCCGCAC TATCCAAGAC TTGGGCATCG GCACATTCCG AGATTTGGCT 
GTAGTTCTGG AAACAGCTCC TGTCCTGACT GCGCTGGACA TCTTTGTGGA CCGACGTGTG 
TCTGCACTGC CTGTGGTCAA TGAATCTGGT CAGGTCGTGG GCCTCTACTC CCGCTTTGAT 
GTCATTCACC TGGCTGCCCA GCAAACCTAC AACCACCTAG ACATGAGTGT GGGAGAAGCT 
CTGAGACAGA GGACACTGTG TCTGGAGGGA GTTCTCTCCT GCCAGCCCCA CGAGAGCCTA 
GGTGAAGTCA TTGACAGGAT CGCACGGGAA CAGGTGCATA GGCTGGTGTT GGTGGATGAG 
ACCCAGCATC TTCTGGGCGT GGTCTCCCTC TCTGACATAC TTCAAGCACT GGTACTCAGC 
CCTGCTGGCA TCGATGCCCT CAGCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Activation of Skeletal Muscle AMPK Promotes Glucose Disposal and Glucose Lowering in Non-human Primates and Mice.
Cell metabolism25(5)1147-1159.e10(2017 May)
Cokorinos EC,Delmore J,Reyes AR,Albuquerque B,Kj?bsted R,J?rgensen NO,Tran JL,Jatkar A,Cialdea K,Esquejo RM,Meissen J,Calabrese MF,Cordes J,Moccia R,Tess D,Salatto CT,Coskran TM,Opsahl AC,Flynn D,Blatnik M,Li W,Kindt E,Foretz M,Viollet B,Ward J,Kurumbail RG,Kalgutkar AS,Wojtaszewski JFP,Cameron KO,Miller RA

Enhanced Muscle Insulin Sensitivity After Contraction/Exercise Is Mediated by AMPK.
Diabetes66(3)598-612(2017 03)
Kj?bsted R,Munk-Hansen N,Birk JB,Foretz M,Viollet B,Bj?rnholm M,Zierath JR,Treebak JT,Wojtaszewski JF

AMPK?3 is dispensable for skeletal muscle hypertrophy induced by functional overload.
American journal of physiology. Endocrinology and metabolism310(6)E461-72(2016 Mar)
Riedl I,Osler ME,Bj?rnholm M,Egan B,Nader GA,Chibalin AV,Zierath JR

Prior AICAR stimulation increases insulin sensitivity in mouse skeletal muscle in an AMPK-dependent manner.
Diabetes64(6)2042-55(2015 Jun)
Kj?bsted R,Treebak JT,Fentz J,Lantier L,Viollet B,Birk JB,Schjerling P,Bj?rnholm M,Zierath JR,Wojtaszewski JF

Gene discovery by microarray: identification of novel genes induced during growth factor-mediated muscle cell survival and differentiation.
Genomics84(5)876-89(2004 Nov)
Kuninger D,Kuzmickas R,Peng B,Pintar JE,Rotwein P

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Prkag3 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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