Pgap1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pgap1
Entrez Gene ID	241062
Full Name	post-GPI attachment to proteins 1
Synonyms	5033403E17Rik,9030223K07Rik,A530084K22,D230012E17Rik,oto
General protein information	Preferred Names post-GPI attachment to proteins 1 Names GPI inositol-deacylase post-GPI attachment to proteins factor 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1\|1 C1.1-C1.2

mRNA and Protein(s)

mRNA	Protein	Name
NM_001163314.2	NP_001156786.1	GPI inositol-deacylase

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PGAP1	XP_001168679.1
Canis lupus familiaris (dog)	PGAP1	XP_850469.1
Bos taurus (cattle)	PGAP1	NP_001192479.1
Gallus gallus (chicken)	PGAP1	XP_003641623.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011299	XP_309348.4
Xenopus tropicalis (tropical clawed frog)	LOC101732527	XP_004918323.1
Mus musculus (house mouse)	Pgap1	NP_001156786.1
	LOC696475	XP_002808064.1
Homo sapiens (human)	PGAP1	NP_079265.2
Rattus norvegicus (Norway rat)	Pgap1	NP_973719.1
Danio rerio (zebrafish)	pgap1	XP_001922432.2
Drosophila melanogaster (fruit fly)	CG3160	NP_572245.1

Gene Ontology
Bibliography

Related articles in PubMed

Abnormal Paraventricular Nucleus of Hypothalamus and Growth Retardation Associated with Loss of Nuclear Receptor Gene COUP-TFII.
Feng S, Xing C, Shen T, Qiao Y, Wang R, Chen J, Liao J, Lu Z, Yang X, Abd-Allah SM, Li J, Jing N, Tang K
Scientific reports7(1)5282(2017 Jul)

High-throughput screening of mouse gene knockouts identifies established and novel skeletal phenotypes.
Brommage R, Liu J, Hansen GM, Kirkpatrick LL, Potter DG, Sands AT, Zambrowicz B, Powell DR, Vogel P
Bone research214034(2014)

Defects in GPI biosynthesis perturb Cripto signaling during forebrain development in two new mouse models of holoprosencephaly.
McKean DM, Niswander L
Biology open1(9)874-83(2012 Sep)

A mouse knockout library for secreted and transmembrane proteins.
Tang T, Li L, Tang J, Li Y, Lin WY, Martin F, Grant D, Solloway M, Parker L, Ye W, Forrest W, Ghilardi N, Oravecz T, Platt KA, Rice DS, Hansen GM, Abuin A, Eberhart DE, Godowski P, Holt KH, Peterson A, Zambrowicz BP, de Sauvage FJ
Nature biotechnology28(7)749-55(2010 Jul)

Wnt signaling is regulated by endoplasmic reticulum retention.
Zoltewicz JS, Ashique AM, Choe Y, Lee G, Taylor S, Phamluong K, Solloway M, Peterson AS
PloS one4(7)e6191(2009 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PGAP1-deficient spermatozoa showed weak attachment to the zona pellucida and a severely diminished rate of fertilization
Title: PGAP1 knock-out mice show otocephaly and male infertility.

The following Pgap1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pgap1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu08886	NM_001163314.2 Latest version!	Mus musculus post-GPI attachment to proteins 1 (Pgap1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$545.30 $779.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pgap1 ( NM_001163314.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001156786.1 Mus musculus post-GPI attachment to proteins 1 (Pgap1), mRNA.

Price & Availability↑

CloneID	OMu08886
Clone ID Related Accession (Same CDS sequence)	NM_001163314.2
Accession Version	NM_001163314.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	GPI inositol-deacylase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK041133.1, AK138171.1, CK392830.1, AC124538.4, CF745447.1, CN536101.1, BM250484.2, AK017146.1, BG803764.1, BM943932.1, CV558383.1, AK033485.1 and AK141991.1. On Oct 25, 2011 this sequence version replaced NM_001163314.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTTCTCC ACTCAGTCAA CCTCTGGAAT CTGGCGTTCT ATGTCTTCAT GGTGTTTCTG 
GCGACCCTGG GGCTGTGGGA TGTCTTCTTC GGCTTCGAGG AAAACAAGTG CAGCATGAGC 
TACATGTTCG AGTACCCCGA GTACCAGAAA ATAGAGCTCC CAAAGAAACT GACAAAGCGT 
TATCCAGCAT ATGAGTTATA TCTTTACGGA GAAGGATCGT ACGCTGAAGA ACACAAAATT 
CTCCCTTTGA CAGGAATTCC TGTTCTCTTT CTTCCTGGTA ATGCTGGAAG CTATAAGCAA 
GTTCGATCTA TCGGCTCTAT TGCACTTAGG AAAGCAGAAG ACATCGACTT CAAGTACCAC 
TTCGACTTCT TCAGTGTCAA CTTCAATGAA GAACTGGTGG CATTGTATGG TGGAAGCCTT 
CAGAAGCAGA CCAAGTTTGT ACACGAGTGC ATTAAAGCGA TTCTCAAACT CTACAAGGGT 
CAAGAATTTG CTCCTACAAG TGTGGCAATA ATTGGTCACT CAATGGGTGG CCTTGTTGCC 
AGAGCATTGC TTACACTGAA AAATTTTAAG CAAGATCTGA TCAATCTTCT TGTTACACAA 
GCTACTCCTC ATGTGGCCCC TGTGATGCCA TTAGATCGTT TCATTACAGA GTTTTATATG 
AATGTAAACA ACTATTGGAT TTTAAATGCT CGGCACATAA ATTTGACTAC ACTTTCTGTG 
GCTGGAGGAT TCCGGGATTA CCAAGTTCGT TCAGGACTGA CTTTTCTACC AAAATTGAGC 
CATTACACCA GTGCCTTATC TGTTGTGAGC TCAGCAGTGC CTAAGACCTG GGTCTCAACA 
GACCACCTCT CCATCGTGTG GTGTAAACAA TTGCAGTTGA CTACAATTCG AGCGTTCTTT 
GATCTTATTG ATGCCGATAC CAAACAAATA ACTCAAAAGC CTAAGAAGAA ATTGTCCGTT 
TTGAATCACC ATTTTATAAG ACACCCAGCT AAACAGTTTG AGGAAAATCC GTCCATAATT 
TCTGACCTAA CAGGGACATC TATGTGGGTT CCAGTAAAAG TGTCCAGATG GTCCTATGTG 
GCTTATAATG AATCTGATAA GATTTACTTT GCATTTCCTC TTGCAAATCA TAGAAAAATC 
TACACTCATG CCTATTGTCA GAGCACCATG CTGGATACAA ATAGTTGGAT TTTTGGCTGC 
ATAAACAGCA CTTCCATGTG CCGGCAAGGG GTTGATTTGT CATGGAAAGC TGAGCTGTTA 
CCAACAATTA AGTCTCTGAC GCTAAGACTT CAAGACTATC CATCCTTGTC GCATATTGTT 
GTCTATGTGC CATCTGTCCA CGGAAGTAAG TTTGTTGTCG ACTGTGAATT CTTTAAGAAA 
GAAGCGAGAT CCATGCAGCT CCCTGTGACT CACCTTTTTT CCTTTGGACT GTCTTCCAGG 
AAGGTGACGT TAAATACAAA CGGCCTATAC TACAACATAG AGCTGCTGAA CTTTGGACAG 
ATATACCAAG CTTTTAAAGT CAACGTGGTC AGCAAGTGCA CTGGAAGCAA AGAAGAAATA 
ACCAGTATCT ATAAACTTCA TATCCCCTGG TCCTATGAAG ATTCACTAAC CATCGCACAG 
GTTCCATCTT CCACAGACAT TTCTCTGAAG CTTCATGTTG CTCAGCCAGA AAATGACAGC 
CATGTGGCAC TGTTAAAAAT GTACACGTCG TCAGACTGTC AGTATGAGGT GACAATTAAG 
ACTTCCTTCC CACAAATACT TGGACAGGTA GTTAGGTTTC ATGGTGGAGC CCTTCCTGCC 
TACGTTGTAT CTAGCATCCT TCTTGCGTAT GGAGGACAGT TATACTCACT CCTCTCAACA 
GGTTATTGCT TAGAATATTC TACCATATTG GATAAAGAAG CCAAACCATA CAAAGTTGAC 
CCATTTGTAA TTATGATTAA GTTTTTGTTG GGGTATAAAT GGTTTAAGGA ATTATGGGAT 
GCAGTGTTAT TACCGGAATT AGATGCCATC GTTTTGACTA GCCAGAGTAT GTGTTTCCCT 
CTTGTATCCT TGATTCTCTT TCTGTTTGGA ACATGTACTG CATACTGGAG TGGTCTACTC 
TCTTCTACAT CTGTGCAGCT TCTTTCTTCA TTGTGGCTAG CTCTGAAAAG ACCTGCTGAA 
CTTCCTAAAG ATATCAAGGT GATGTCACCA GACTTGCCTG TTTTGACAGT TGTCTTTCTC 
ATAGTGAGTT GGACAACTTG TGGAGCACTT GCCATACTTC TGTCTTATCT GTACTATGTG 
TTTAAGGTTG TTCATCTGCA AGCCAGCCTA ACTACATTTA AAAATAATCA GCCAGTGAAT 
CCCAAACACT CTAGAAGAAG TGAAAAAAAG TCCAACCACC ATAAAGACTC TGCTGTACAA 
AGTCTTCGCT TATGTGCTAA TGATGCTGAA GACAGTCTTC GCATGCACAG CACCGTGATT 
AACTTGCTGA CGTGGGTTGT GCTGCTCAGC ATGCCGTCTC TAATCTACTG GTTAAAGAAC 
CTTAGGTATT ATTTTAAACT TAGTCCTGAC CCATGCAAAC CCCTGGCATT TCTCCTTATT 
CCAGCTATAG CGATTCTCGG AAACACTCAC ACTGTTTCAG TAAAGTCCAG TAAATTGCTG 
AAGACCGTCT CCCAGTTCCC ACTTCCTCTG GCTGTTGGCG TGATTGCTTT TGGCTCCTCC 
CACTTATACA GGGTCCCGTG TTTTGTGATC ATTCCTCTTG TGTTCCATGC GTTATGCAAC 
TTTATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001156786.1
CDS	105..2873
Translation	MFLHSVNLWNLAFYVFMVFLATLGLWDVFFGFEENKCSMSYMFEYPEYQKIELPKKLTKRYPAYELYLYGEGSYAEEHKILPLTGIPVLFLPGNAGSYKQVRSIGSIALRKAEDIDFKYHFDFFSVNFNEELVALYGGSLQKQTKFVHECIKAILKLYKGQEFAPTSVAIIGHSMGGLVARALLTLKNFKQDLINLLVTQATPHVAPVMPLDRFITEFYMNVNNYWILNARHINLTTLSVAGGFRDYQVRSGLTFLPKLSHYTSALSVVSSAVPKTWVSTDHLSIVWCKQLQLTTIRAFFDLIDADTKQITQKPKKKLSVLNHHFIRHPAKQFEENPSIISDLTGTSMWVPVKVSRWSYVAYNESDKIYFAFPLANHRKIYTHAYCQSTMLDTNSWIFGCINSTSMCRQGVDLSWKAELLPTIKSLTLRLQDYPSLSHIVVYVPSVHGSKFVVDCEFFKKEARSMQLPVTHLFSFGLSSRKVTLNTNGLYYNIELLNFGQIYQAFKVNVVSKCTGSKEEITSIYKLHIPWSYEDSLTIAQVPSSTDISLKLHVAQPENDSHVALLKMYTSSDCQYEVTIKTSFPQILGQVVRFHGGALPAYVVSSILLAYGGQLYSLLSTGYCLEYSTILDKEAKPYKVDPFVIMIKFLLGYKWFKELWDAVLLPELDAIVLTSQSMCFPLVSLILFLFGTCTAYWSGLLSSTSVQLLSSLWLALKRPAELPKDIKVMSPDLPVLTVVFLIVSWTTCGALAILLSYLYYVFKVVHLQASLTTFKNNQPVNPKHSRRSEKKSNHHKDSAVQSLRLCANDAEDSLRMHSTVINLLTWVVLLSMPSLIYWLKNLRYYFKLSPDPCKPLAFLLIPAIAILGNTHTVSVKSSKLLKTVSQFPLPLAVGVIAFGSSHLYRVPCFVIIPLVFHALCNFM

Target ORF information:

RefSeq Version	NM_001163314.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus post-GPI attachment to proteins 1 (Pgap1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001163314.2

ORF Insert Sequence:

ATGTTTCTCC ACTCAGTCAA CCTCTGGAAT CTGGCGTTCT ATGTCTTCAT GGTGTTTCTG 
GCGACCCTGG GGCTGTGGGA TGTCTTCTTC GGCTTCGAGG AAAACAAGTG CAGCATGAGC 
TACATGTTCG AGTACCCCGA GTACCAGAAA ATAGAGCTCC CAAAGAAACT GACAAAGCGT 
TATCCAGCAT ATGAGTTATA TCTTTACGGA GAAGGATCGT ACGCTGAAGA ACACAAAATT 
CTCCCTTTGA CAGGAATTCC TGTTCTCTTT CTTCCTGGTA ATGCTGGAAG CTATAAGCAA 
GTTCGATCTA TCGGCTCTAT TGCACTTAGG AAAGCAGAAG ACATCGACTT CAAGTACCAC 
TTCGACTTCT TCAGTGTCAA CTTCAATGAA GAACTGGTGG CATTGTATGG TGGAAGCCTT 
CAGAAGCAGA CCAAGTTTGT ACACGAGTGC ATTAAAGCGA TTCTCAAACT CTACAAGGGT 
CAAGAATTTG CTCCTACAAG TGTGGCAATA ATTGGTCACT CAATGGGTGG CCTTGTTGCC 
AGAGCATTGC TTACACTGAA AAATTTTAAG CAAGATCTGA TCAATCTTCT TGTTACACAA 
GCTACTCCTC ATGTGGCCCC TGTGATGCCA TTAGATCGTT TCATTACAGA GTTTTATATG 
AATGTAAACA ACTATTGGAT TTTAAATGCT CGGCACATAA ATTTGACTAC ACTTTCTGTG 
GCTGGAGGAT TCCGGGATTA CCAAGTTCGT TCAGGACTGA CTTTTCTACC AAAATTGAGC 
CATTACACCA GTGCCTTATC TGTTGTGAGC TCAGCAGTGC CTAAGACCTG GGTCTCAACA 
GACCACCTCT CCATCGTGTG GTGTAAACAA TTGCAGTTGA CTACAATTCG AGCGTTCTTT 
GATCTTATTG ATGCCGATAC CAAACAAATA ACTCAAAAGC CTAAGAAGAA ATTGTCCGTT 
TTGAATCACC ATTTTATAAG ACACCCAGCT AAACAGTTTG AGGAAAATCC GTCCATAATT 
TCTGACCTAA CAGGGACATC TATGTGGGTT CCAGTAAAAG TGTCCAGATG GTCCTATGTG 
GCTTATAATG AATCTGATAA GATTTACTTT GCATTTCCTC TTGCAAATCA TAGAAAAATC 
TACACTCATG CCTATTGTCA GAGCACCATG CTGGATACAA ATAGTTGGAT TTTTGGCTGC 
ATAAACAGCA CTTCCATGTG CCGGCAAGGG GTTGATTTGT CATGGAAAGC TGAGCTGTTA 
CCAACAATTA AGTCTCTGAC GCTAAGACTT CAAGACTATC CATCCTTGTC GCATATTGTT 
GTCTATGTGC CATCTGTCCA CGGAAGTAAG TTTGTTGTCG ACTGTGAATT CTTTAAGAAA 
GAAGCGAGAT CCATGCAGCT CCCTGTGACT CACCTTTTTT CCTTTGGACT GTCTTCCAGG 
AAGGTGACGT TAAATACAAA CGGCCTATAC TACAACATAG AGCTGCTGAA CTTTGGACAG 
ATATACCAAG CTTTTAAAGT CAACGTGGTC AGCAAGTGCA CTGGAAGCAA AGAAGAAATA 
ACCAGTATCT ATAAACTTCA TATCCCCTGG TCCTATGAAG ATTCACTAAC CATCGCACAG 
GTTCCATCTT CCACAGACAT TTCTCTGAAG CTTCATGTTG CTCAGCCAGA AAATGACAGC 
CATGTGGCAC TGTTAAAAAT GTACACGTCG TCAGACTGTC AGTATGAGGT GACAATTAAG 
ACTTCCTTCC CACAAATACT TGGACAGGTA GTTAGGTTTC ATGGTGGAGC CCTTCCTGCC 
TACGTTGTAT CTAGCATCCT TCTTGCGTAT GGAGGACAGT TATACTCACT CCTCTCAACA 
GGTTATTGCT TAGAATATTC TACCATATTG GATAAAGAAG CCAAACCATA CAAAGTTGAC 
CCATTTGTAA TTATGATTAA GTTTTTGTTG GGGTATAAAT GGTTTAAGGA ATTATGGGAT 
GCAGTGTTAT TACCGGAATT AGATGCCATC GTTTTGACTA GCCAGAGTAT GTGTTTCCCT 
CTTGTATCCT TGATTCTCTT TCTGTTTGGA ACATGTACTG CATACTGGAG TGGTCTACTC 
TCTTCTACAT CTGTGCAGCT TCTTTCTTCA TTGTGGCTAG CTCTGAAAAG ACCTGCTGAA 
CTTCCTAAAG ATATCAAGGT GATGTCACCA GACTTGCCTG TTTTGACAGT TGTCTTTCTC 
ATAGTGAGTT GGACAACTTG TGGAGCACTT GCCATACTTC TGTCTTATCT GTACTATGTG 
TTTAAGGTTG TTCATCTGCA AGCCAGCCTA ACTACATTTA AAAATAATCA GCCAGTGAAT 
CCCAAACACT CTAGAAGAAG TGAAAAAAAG TCCAACCACC ATAAAGACTC TGCTGTACAA 
AGTCTTCGCT TATGTGCTAA TGATGCTGAA GACAGTCTTC GCATGCACAG CACCGTGATT 
AACTTGCTGA CGTGGGTTGT GCTGCTCAGC ATGCCGTCTC TAATCTACTG GTTAAAGAAC 
CTTAGGTATT ATTTTAAACT TAGTCCTGAC CCATGCAAAC CCCTGGCATT TCTCCTTATT 
CCAGCTATAG CGATTCTCGG AAACACTCAC ACTGTTTCAG TAAAGTCCAG TAAATTGCTG 
AAGACCGTCT CCCAGTTCCC ACTTCCTCTG GCTGTTGGCG TGATTGCTTT TGGCTCCTCC 
CACTTATACA GGGTCCCGTG TTTTGTGATC ATTCCTCTTG TGTTCCATGC GTTATGCAAC 
TTTATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Abnormal Paraventricular Nucleus of Hypothalamus and Growth Retardation Associated with Loss of Nuclear Receptor Gene COUP-TFII.
Scientific reports7(1)5282(2017 Jul)
Feng S,Xing C,Shen T,Qiao Y,Wang R,Chen J,Liao J,Lu Z,Yang X,Abd-Allah SM,Li J,Jing N,Tang K

High-throughput screening of mouse gene knockouts identifies established and novel skeletal phenotypes.
Bone research214034(2014)
Brommage R,Liu J,Hansen GM,Kirkpatrick LL,Potter DG,Sands AT,Zambrowicz B,Powell DR,Vogel P

Defects in GPI biosynthesis perturb Cripto signaling during forebrain development in two new mouse models of holoprosencephaly.
Biology open1(9)874-83(2012 Sep)
McKean DM,Niswander L

A mouse knockout library for secreted and transmembrane proteins.
Nature biotechnology28(7)749-55(2010 Jul)
Tang T,Li L,Tang J,Li Y,Lin WY,Martin F,Grant D,Solloway M,Parker L,Ye W,Forrest W,Ghilardi N,Oravecz T,Platt KA,Rice DS,Hansen GM,Abuin A,Eberhart DE,Godowski P,Holt KH,Peterson A,Zambrowicz BP,de Sauvage FJ

Wnt signaling is regulated by endoplasmic reticulum retention.
PloS one4(7)e6191(2009 Jul)
Zoltewicz JS,Ashique AM,Choe Y,Lee G,Taylor S,Phamluong K,Solloway M,Peterson AS

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Pgap1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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