Pik3c2b cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pik3c2b
Entrez Gene ID	240752
Full Name	phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 beta
Synonyms	C330011J12Rik,Gm199,PI3K-C2beta,PI3KC2beta
General protein information	Preferred Names phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 beta Names phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit beta phosphoinositide-3-kinase, class 2, beta polypeptide
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1\|1 E4

mRNA and Protein(s)

mRNA	Protein	Name
XM_006529533.3	XP_006529596.1	phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit beta isoform X2
XM_006529531.3	XP_006529594.1	phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit beta isoform X1
XM_006529532.2	XP_006529595.1	phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit beta isoform X1
NM_001099276.2	NP_001092746.2	phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit beta
XM_006529531.4	XP_006529594.1	phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit beta isoform X1
XM_006529533.4	XP_006529596.1	phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit beta isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	PIK3C2B	NP_002637.3
	PIK3C2B	XP_002802010.1
Mus musculus (house mouse)	Pik3c2b	NP_001092746.2
Rattus norvegicus (Norway rat)	Pik3c2b	NP_001099421.1
Danio rerio (zebrafish)	pik3c2b	XP_003199535.1
Pan troglodytes (chimpanzee)	PIK3C2B	XP_514126.3
Canis lupus familiaris (dog)	PIK3C2B	XP_005640823.1
Bos taurus (cattle)	PIK3C2B	XP_002693928.3
Gallus gallus (chicken)	PIK3C2B	XP_417956.3

Gene Ontology
Bibliography

Related articles in PubMed

Phosphatidlyinositol-3-kinase C2 beta (PI3KC2?) is a potential new target to treat IgE mediated disease.
Srivastava S, Li Z, Skolnik EY
PloS one12(8)e0183474(2017)

PIK3C2B inhibition improves function and prolongs survival in myotubular myopathy animal models.
Sabha N, Volpatti JR, Gonorazky H, Reifler A, Davidson AE, Li X, Eltayeb NM, Dall'Armi C, Di Paolo G, Brooks SV, Buj-Bello A, Feldman EL, Dowling JJ
The Journal of clinical investigation126(9)3613-25(2016 Sep)

Role of protein kinase A and class II phosphatidylinositol 3-kinase C2? in the downregulation of KCa3.1 channel synthesis and membrane surface expression by lyso-globotriaosylceramide.
Choi JY, Park S
Biochemical and biophysical research communications470(4)907-12(2016 Feb)

Inactivation of the Class II PI3K-C2? Potentiates Insulin Signaling and Sensitivity.
Alliouachene S, Bilanges B, Chicanne G, Anderson KE, Pearce W, Ali K, Valet C, Posor Y, Low PC, Chaussade C, Scudamore CL, Salamon RS, Backer JM, Stephens L, Hawkins PT, Payrastre B, Vanhaesebroeck B
Cell reports13(9)1881-94(2015 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Pik3c2b is a genetic modifier of Mtm1 mutation with a role in function and survival in myotubular myopathy animal models
Title: PIK3C2B inhibition improves function and prolongs survival in myotubular myopathy animal models.

Modulation of PKA and PI3KC2beta activity to control of KCa3.1 channel expression can be an alternative important target to attenuate ascending thoracic aortic aneurysms in Fabry disease.
Title: Role of protein kinase A and class II phosphatidylinositol 3-kinase C2? in the downregulation of KCa3.1 channel synthesis and membrane surface expression by lyso-globotriaosylceramide.

study found that TRIM27 negatively regulates FcepsilonRI activation of KCa3.1 and downstream signaling by ubiquitinating and inhibiting PI3KC2beta
Title: Phosphatidylinositol-3-kinase C2? and TRIM27 function to positively and negatively regulate IgE receptor activation of mast cells.

These findings provide a unique mechanism for regulating class II PI3Ks, and identify TRIM27 as a previously undescribed negative regulator of CD4 T cells.
Title: Tripartite motif containing protein 27 negatively regulates CD4 T cells by ubiquitinating and inhibiting the class II PI3K-C2?.

Our findings demonstrate for the first time that the PI3K-C2beta isoform associates with clathrin and thus provides a link between receptor mediated intracellular signalling and clathrin coated vesicle transport.
Title: The N-terminus of phosphoinositide 3-kinase-C2beta regulates lipid kinase activity and binding to clathrin.

The following Pik3c2b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pik3c2b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu28198	XM_006529533.4 Latest version!	Mus musculus phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 beta (Pik3c2b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu02575	XM_006529531.4 Latest version!	Mus musculus phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 beta (Pik3c2b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu02575	NM_001099276.2 Latest version!	Mus musculus phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 beta (Pik3c2b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu02575	XM_006529532.2 Latest version!	Mus musculus phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 beta (Pik3c2b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
View Old Accession Versions >>
OMu02575	XM_006529531.3	Mus musculus phosphoinositide-3-kinase, class 2, beta polypeptide (Pik3c2b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu28198	XM_006529533.3	Mus musculus phosphoinositide-3-kinase, class 2, beta polypeptide (Pik3c2b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pik3c2b ( XM_006529533.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529596.1 Mus musculus phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 beta (Pik3c2b), transcript variant X3, mRNA.

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CloneID	OMu28198
Clone ID Related Accession (Same CDS sequence)	XM_006529533.3 , XM_006529533.4
Accession Version	XM_006529533.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4236bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit beta isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006529533.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCAGT GGTCCCCCCC ACCTCAGCTC GGGGCAGGGG CACAGCCTGC TGGGAGGGGA 
GACCCCTTGA TCTCCGCTCC TATACCCCCA CCCCGCCTTC GCCGGGCTTC CGGCTGGAAG 
GATCCTACTT ATCCCAGGCC CACTGAGCCA TCTCTGGAAA GGGCTAGCTC CAAATGCCAG 
CCTGGTACCC CCGCTGCCAG CCTCTGCCTG GCAGACTCCC CCGACCAGCA TCGGACGATG 
CCACCAGGGC CTGCCAGCCG GGCAAACGTG GGCTCTCGGC CCCACACTGT TGCCAATGGC 
CACGAATTGT TTGAGGTCTC AGAGGAGAGA GATGAGGAAG TTGCTGCGTT CTGCCACATG 
CTGGATATCC TTCGAACGGG CTCTGATATC CAGGACTATT CCCTAACTGG GTGTGTCTGG 
AGCACTGTCA CCCCAAGTCC AGAACACCTT GGGGATGAGG TCAACCTGAA GGTGACAGTG 
TTGTGTGACA GTCTCCGGGA GCCACTCACC TTTACCTGCA ACTGTTCCTC CACCGTGGAC 
CTGCTCATCC ACCAGACCCT CTGCTACACT CACGATGAGC TGAGGGAAGT GGACGTGGGC 
GACTTTGTGC TGAAGCCCTG TGGGCTGGAG GAGTTCTTGC AAAATAAGCA CGCCCTGGGA 
AGCCATGAGT ATATCCAATA TTGCCGCAAG TTTGATATCA GCATCCGGCT ACAGCTGATG 
GAGCAGAAGG CTATCCGGAG CGACCTGGCC CGGACGGTGA ATGACGACCA GAGCCCTTCC 
ACCTTGAACT ACCTCATCCA TCTCCAAGAG AGGCCAGTCA AGCAGACCAT CAGCAGGCAG 
GCCTTGAGTC TTCTGTTTGA CACTTACCAC AATGAGGTGG ACGCCTTCCT GCTGGCTGAT 
GGGGATTTCC CACTGAAGGC TGACAGGGTG GTCCAGTCTG TCAAGGCCAT TTGCAATGCT 
CTGGCTGCTG TGGAGACTCC CGAGATCACG AGCGCTCTCA ACCAGCTGCC TCCCTGCCCC 
TCGCGCATGC AGCCAAAAAT CCAGAAGGAT CCCAGCGTGT TGAGTGTGAG GGAAAACCGA 
GAGAAGGTGG TAGAAGCCTT GACAGCGGCC ATCTTGGACT TGGTGGAGCT GTACTGCAGC 
ACATTCAATG CAGACTTCCA GACGGCGGTG CCTGGGAGCC GAAAGCATGA TTTGGTTCAA 
GAGGCCTGCC ACTTCCCTGG GGCCCTGGCC TTCACTGTCT ATGGCACTCA CCGTATTCCC 
ATCATCTGGG CTACCAGCTA TGAAGATTTC TACCTCTCCT GCTCCCTCAG CCACGGTGGC 
AAGGAACTTT GCAGCCCCCT GCAGACCCGG CGGGCTCACT TCTCCAAGTA CCTGTTCCAC 
CTCATCATCT GGGACCAGCA GATCTGCTTT CCAGTGCAGG TGAACAGGCT ACCTCGGGAG 
ACTCTGCTGT GTGCCACACT CTATGCCCTG CCCGTTCCCC CTCCTGGGGG TTCCTCTGAA 
GCGAATAAGC AGAAGCGGGT GCCTGAAGCT CTGGGTTGGG TCACTACCCC TCTCTTCAAC 
TTCAGGCAAG TCTTGACCTG TGGTCGAAAG CTCCTGGGCT TGTGGCCAGC GACACAGGAA 
AACTCCGGTG CCCGCTGGAG TGCGCCTAAT TTCCACCAGC CAGACAGTGT TATCCTGCAG 
ATTGACTTCC CCAGCTCGGC CTTTGACATC AAGTTCACCA GCCCCCCTGG AGACAAGTTC 
AGCCCCCGCT ATGAGTTTGG CAGTCTCCGG GAGGAGGACC AGCGCAAACT TAAAGACATT 
ACGCAGAAGG AGTCCCTATA CTGGCTCACG GATGCTGACA AGAAGCAGCT GTGGGAGAAA 
CGCTATTACT GCCACACTGA GGTGAGCTCA CTCCCCCTGG TTCTCGCCAG TGCACCCAGC 
TGGGAGTGGG CTTGCCTGCC GGACATCTAT GCTCTCCTGC AACAGTGGAC TCACATGAAC 
CACCAAGATG CCCTGGGACT CCTGCATGCC ACCTTCCCGG ACCAGGAGGT GCGGCGGATG 
GCCGTGCAGT GGATCGGCTC CCTCTCAGAT GCGGAGCTGC TGGACTACCT GCCCCAGCTA 
GTCCAGGCCC TGAAGTATGA GTGCTACCTG GATAGCCCCT TGGTGCGCTT TCTCCTGAAA 
CGAGCCATCT CTGACCTGCG AGTGACCCAC TACTTCTTCT GGTTACTGAA GGACAGCCTC 
AAGGACTCTC AGTTCAGTAT CCGCTACCAA TACCTGCTAG CAGCCCTGCT GTGCTGCTGT 
GGCAAGGGAC TGAGGGAGGA GTTTAACCGC CAGTGCTGGC TTGTCAACAC CCTGGCCAAA 
CTGGCCCAGC AGGTCCGAGA GGCCACCCCA TCCGCCCGCC AGGGCATCCT TCGTGTGGGC 
TTGGAGGAGG TGAAGCAGTT CTTTGCCCTC AATGGTTCGT GCCGTCTGCC GCTCAGTCCC 
AGTCTCCTGG TGAAGGGCAT CGTGCCCAGG GACTGCTCCT ACTTCAATTC CAACGCAGTG 
CCCCTGAAGC TTGCCTTCCA AAATGTGGAT CCGCTGGGCG AGAACATCAG AGTCATCTTT 
AAGTGTGGGG ATGATCTTCG TCAGGACATG CTCACCCTGC AGATGATTCG CATCATGAGC 
AAGATCTGGG TACAGGAGGG GCTGGACATG CGCATGGTCA TCTTCCGCTG CTTCTCCACT 
GGCCGAGGAA AAGGGATGGT AGAGATGATA CCCAATGCAG AGACACTGCG CAAGATCCAG 
GTGGAACATG GGGTGACTGG CTCCTTCAAG GACCGTCCCC TGGCCGACTG GCTACAAAAA 
CACAACCCTG GGGAGGATGA ATATGAAAAG GCTGTGGAGA ACTTCATCTA CTCTTGTGCT 
GGCTGCTGTG TAGCTACATA CATCTTGGGC ATCTGTGACA GACACAATGA CAACATCATG 
CTAAAGACCA CGGGCCACAT GTTCCATATA GATTTCGGCC GCTTCCTGGG TCACGCACAG 
ATGTTTGGTA ACATCAAGAG GGACCGTGCC CCGTTTGTCT TCACCTCAGA CATGGCGTAT 
GTCATCAATG GGGGTGACAA GCCTTCCAGC CGCTTCCATG ATTTTGTTGA CCTTTGCTGC 
CAAGCCTACA ACCTCATTCG CAAGCACACC CACCTCTTCC TCAACCTTCT GGGCCTGATG 
CTGTCCTGTG GAATCCCCGA GCTCTCAGAC CTGGAGGACC TCAAGTATGT GTATGATGCC 
CTGAGACCTC AGGACACAGA GGCCAATGCC ACCACCTACT TCACAAGGTT GATTGAGTCC 
AGCCTGGGCA GCGTAGCCAC GAAACTCAAC TTTTTCATCC ATAACCTGGC TCAGATGAAG 
TTCACAGGCT CAGATGACCG GCTGACCCTC TCCTTCGCTC CCCGGACACA CACTCTCAAG 
AGCTCTGGCC GCATCCGAGA TGTCTTCCTC TGCCGACATG AGAAGATCTT CCACCCCAGC 
AAGGGCTATG TATATGTGGT GAAGGTGATG CGAGAGAATG CTCACGAGGC GACATACATC 
CAGAGGACAT TCGAGGAGTT CCAGGAACTG CACAATAAGC TGCGCCTGCT CTTCCCTTCT 
TCCTTCCTGC CCAGCTTCCC TAGCCGGTTT GTGATTGGCC GCTCCCGGGG AGAGGCAGTG 
GCTGAGAGGA GGAAGGAGGA GTTAAATGGC TACATCTGGC ACCTGATTCA TGCAGCCCCT 
GAGGTGGCTG AGTGTGATCT GGTGTATACT TTCTTCCACC CCCTGCCCCG AGATGAGAAG 
GCTTCAGGCC CCAGCCCAGC TCCGAAGTCC TCAGATGGGA CGTGGGCCCG GCCGGTTGGG 
AAGGTCGGTG GGGAGGTGAA GCTGTCTATC TCCTACAAAA ACAACAAACT CTTCATCATG 
GTGATGCATA TTCGGGGCTT GCAACCGCTC CAGGATGGCA ATGACCCTGA CCCCTATGTA 
AAGATTTACC TCCTTCCTGA CCCTCAGAAA ACCACAAAGA GGAAAACCAA GGTGGCCCGG 
AAAACCTGCA ACCCTACCTA CAATGAGATG CTGGTATATG ATGGGATCCC CAAGGGAGAC 
CTGCAGCAGC GGGAGCTACA GTTGAGTGTG CTGAGTGAGC AAGGATTTTG GGAGAATCTG 
CTTCTCGGGG AGGTGCACAT CCGCCTTCGG GAGCTGGACC TGGCCCAGGA GAAGACCGGC 
TGGTTTGGAC TGGGTTCTCG AGGTCATGGA ACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529596.1
CDS	156..4391
Translation	MRQWSPPPQLGAGAQPAGRGDPLISAPIPPPRLRRASGWKDPTYPRPTEPSLERASSKCQPGTPAASLCLADSPDQHRTMPPGPASRANVGSRPHTVANGHELFEVSEERDEEVAAFCHMLDILRTGSDIQDYSLTGCVWSTVTPSPEHLGDEVNLKVTVLCDSLREPLTFTCNCSSTVDLLIHQTLCYTHDELREVDVGDFVLKPCGLEEFLQNKHALGSHEYIQYCRKFDISIRLQLMEQKAIRSDLARTVNDDQSPSTLNYLIHLQERPVKQTISRQALSLLFDTYHNEVDAFLLADGDFPLKADRVVQSVKAICNALAAVETPEITSALNQLPPCPSRMQPKIQKDPSVLSVRENREKVVEALTAAILDLVELYCSTFNADFQTAVPGSRKHDLVQEACHFPGALAFTVYGTHRIPIIWATSYEDFYLSCSLSHGGKELCSPLQTRRAHFSKYLFHLIIWDQQICFPVQVNRLPRETLLCATLYALPVPPPGGSSEANKQKRVPEALGWVTTPLFNFRQVLTCGRKLLGLWPATQENSGARWSAPNFHQPDSVILQIDFPSSAFDIKFTSPPGDKFSPRYEFGSLREEDQRKLKDITQKESLYWLTDADKKQLWEKRYYCHTEVSSLPLVLASAPSWEWACLPDIYALLQQWTHMNHQDALGLLHATFPDQEVRRMAVQWIGSLSDAELLDYLPQLVQALKYECYLDSPLVRFLLKRAISDLRVTHYFFWLLKDSLKDSQFSIRYQYLLAALLCCCGKGLREEFNRQCWLVNTLAKLAQQVREATPSARQGILRVGLEEVKQFFALNGSCRLPLSPSLLVKGIVPRDCSYFNSNAVPLKLAFQNVDPLGENIRVIFKCGDDLRQDMLTLQMIRIMSKIWVQEGLDMRMVIFRCFSTGRGKGMVEMIPNAETLRKIQVEHGVTGSFKDRPLADWLQKHNPGEDEYEKAVENFIYSCAGCCVATYILGICDRHNDNIMLKTTGHMFHIDFGRFLGHAQMFGNIKRDRAPFVFTSDMAYVINGGDKPSSRFHDFVDLCCQAYNLIRKHTHLFLNLLGLMLSCGIPELSDLEDLKYVYDALRPQDTEANATTYFTRLIESSLGSVATKLNFFIHNLAQMKFTGSDDRLTLSFAPRTHTLKSSGRIRDVFLCRHEKIFHPSKGYVYVVKVMRENAHEATYIQRTFEEFQELHNKLRLLFPSSFLPSFPSRFVIGRSRGEAVAERRKEELNGYIWHLIHAAPEVAECDLVYTFFHPLPRDEKASGPSPAPKSSDGTWARPVGKVGGEVKLSISYKNNKLFIMVMHIRGLQPLQDGNDPDPYVKIYLLPDPQKTTKRKTKVARKTCNPTYNEMLVYDGIPKGDLQQRELQLSVLSEQGFWENLLLGEVHIRLRELDLAQEKTGWFGLGSRGHGT

Target ORF information:

RefSeq Version	XM_006529533.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 beta (Pik3c2b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529533.4

ORF Insert Sequence:

ATGCGGCAGT GGTCCCCCCC ACCTCAGCTC GGGGCAGGGG CACAGCCTGC TGGGAGGGGA 
GACCCCTTGA TCTCCGCTCC TATACCCCCA CCCCGCCTTC GCCGGGCTTC CGGCTGGAAG 
GATCCTACTT ATCCCAGGCC CACTGAGCCA TCTCTGGAAA GGGCTAGCTC CAAATGCCAG 
CCTGGTACCC CCGCTGCCAG CCTCTGCCTG GCAGACTCCC CCGACCAGCA TCGGACGATG 
CCACCAGGGC CTGCCAGCCG GGCAAACGTG GGCTCTCGGC CCCACACTGT TGCCAATGGC 
CACGAATTGT TTGAGGTCTC AGAGGAGAGA GATGAGGAAG TTGCTGCGTT CTGCCACATG 
CTGGATATCC TTCGAACGGG CTCTGATATC CAGGACTATT CCCTAACTGG GTGTGTCTGG 
AGCACTGTCA CCCCAAGTCC AGAACACCTT GGGGATGAGG TCAACCTGAA GGTGACAGTG 
TTGTGTGACA GTCTCCGGGA GCCACTCACC TTTACCTGCA ACTGTTCCTC CACCGTGGAC 
CTGCTCATCC ACCAGACCCT CTGCTACACT CACGATGAGC TGAGGGAAGT GGACGTGGGC 
GACTTTGTGC TGAAGCCCTG TGGGCTGGAG GAGTTCTTGC AAAATAAGCA CGCCCTGGGA 
AGCCATGAGT ATATCCAATA TTGCCGCAAG TTTGATATCA GCATCCGGCT ACAGCTGATG 
GAGCAGAAGG CTATCCGGAG CGACCTGGCC CGGACGGTGA ATGACGACCA GAGCCCTTCC 
ACCTTGAACT ACCTCATCCA TCTCCAAGAG AGGCCAGTCA AGCAGACCAT CAGCAGGCAG 
GCCTTGAGTC TTCTGTTTGA CACTTACCAC AATGAGGTGG ACGCCTTCCT GCTGGCTGAT 
GGGGATTTCC CACTGAAGGC TGACAGGGTG GTCCAGTCTG TCAAGGCCAT TTGCAATGCT 
CTGGCTGCTG TGGAGACTCC CGAGATCACG AGCGCTCTCA ACCAGCTGCC TCCCTGCCCC 
TCGCGCATGC AGCCAAAAAT CCAGAAGGAT CCCAGCGTGT TGAGTGTGAG GGAAAACCGA 
GAGAAGGTGG TAGAAGCCTT GACAGCGGCC ATCTTGGACT TGGTGGAGCT GTACTGCAGC 
ACATTCAATG CAGACTTCCA GACGGCGGTG CCTGGGAGCC GAAAGCATGA TTTGGTTCAA 
GAGGCCTGCC ACTTCCCTGG GGCCCTGGCC TTCACTGTCT ATGGCACTCA CCGTATTCCC 
ATCATCTGGG CTACCAGCTA TGAAGATTTC TACCTCTCCT GCTCCCTCAG CCACGGTGGC 
AAGGAACTTT GCAGCCCCCT GCAGACCCGG CGGGCTCACT TCTCCAAGTA CCTGTTCCAC 
CTCATCATCT GGGACCAGCA GATCTGCTTT CCAGTGCAGG TGAACAGGCT ACCTCGGGAG 
ACTCTGCTGT GTGCCACACT CTATGCCCTG CCCGTTCCCC CTCCTGGGGG TTCCTCTGAA 
GCGAATAAGC AGAAGCGGGT GCCTGAAGCT CTGGGTTGGG TCACTACCCC TCTCTTCAAC 
TTCAGGCAAG TCTTGACCTG TGGTCGAAAG CTCCTGGGCT TGTGGCCAGC GACACAGGAA 
AACTCCGGTG CCCGCTGGAG TGCGCCTAAT TTCCACCAGC CAGACAGTGT TATCCTGCAG 
ATTGACTTCC CCAGCTCGGC CTTTGACATC AAGTTCACCA GCCCCCCTGG AGACAAGTTC 
AGCCCCCGCT ATGAGTTTGG CAGTCTCCGG GAGGAGGACC AGCGCAAACT TAAAGACATT 
ACGCAGAAGG AGTCCCTATA CTGGCTCACG GATGCTGACA AGAAGCAGCT GTGGGAGAAA 
CGCTATTACT GCCACACTGA GGTGAGCTCA CTCCCCCTGG TTCTCGCCAG TGCACCCAGC 
TGGGAGTGGG CTTGCCTGCC GGACATCTAT GCTCTCCTGC AACAGTGGAC TCACATGAAC 
CACCAAGATG CCCTGGGACT CCTGCATGCC ACCTTCCCGG ACCAGGAGGT GCGGCGGATG 
GCCGTGCAGT GGATCGGCTC CCTCTCAGAT GCGGAGCTGC TGGACTACCT GCCCCAGCTA 
GTCCAGGCCC TGAAGTATGA GTGCTACCTG GATAGCCCCT TGGTGCGCTT TCTCCTGAAA 
CGAGCCATCT CTGACCTGCG AGTGACCCAC TACTTCTTCT GGTTACTGAA GGACAGCCTC 
AAGGACTCTC AGTTCAGTAT CCGCTACCAA TACCTGCTAG CAGCCCTGCT GTGCTGCTGT 
GGCAAGGGAC TGAGGGAGGA GTTTAACCGC CAGTGCTGGC TTGTCAACAC CCTGGCCAAA 
CTGGCCCAGC AGGTCCGAGA GGCCACCCCA TCCGCCCGCC AGGGCATCCT TCGTGTGGGC 
TTGGAGGAGG TGAAGCAGTT CTTTGCCCTC AATGGTTCGT GCCGTCTGCC GCTCAGTCCC 
AGTCTCCTGG TGAAGGGCAT CGTGCCCAGG GACTGCTCCT ACTTCAATTC CAACGCAGTG 
CCCCTGAAGC TTGCCTTCCA AAATGTGGAT CCGCTGGGCG AGAACATCAG AGTCATCTTT 
AAGTGTGGGG ATGATCTTCG TCAGGACATG CTCACCCTGC AGATGATTCG CATCATGAGC 
AAGATCTGGG TACAGGAGGG GCTGGACATG CGCATGGTCA TCTTCCGCTG CTTCTCCACT 
GGCCGAGGAA AAGGGATGGT AGAGATGATA CCCAATGCAG AGACACTGCG CAAGATCCAG 
GTGGAACATG GGGTGACTGG CTCCTTCAAG GACCGTCCCC TGGCCGACTG GCTACAAAAA 
CACAACCCTG GGGAGGATGA ATATGAAAAG GCTGTGGAGA ACTTCATCTA CTCTTGTGCT 
GGCTGCTGTG TAGCTACATA CATCTTGGGC ATCTGTGACA GACACAATGA CAACATCATG 
CTAAAGACCA CGGGCCACAT GTTCCATATA GATTTCGGCC GCTTCCTGGG TCACGCACAG 
ATGTTTGGTA ACATCAAGAG GGACCGTGCC CCGTTTGTCT TCACCTCAGA CATGGCGTAT 
GTCATCAATG GGGGTGACAA GCCTTCCAGC CGCTTCCATG ATTTTGTTGA CCTTTGCTGC 
CAAGCCTACA ACCTCATTCG CAAGCACACC CACCTCTTCC TCAACCTTCT GGGCCTGATG 
CTGTCCTGTG GAATCCCCGA GCTCTCAGAC CTGGAGGACC TCAAGTATGT GTATGATGCC 
CTGAGACCTC AGGACACAGA GGCCAATGCC ACCACCTACT TCACAAGGTT GATTGAGTCC 
AGCCTGGGCA GCGTAGCCAC GAAACTCAAC TTTTTCATCC ATAACCTGGC TCAGATGAAG 
TTCACAGGCT CAGATGACCG GCTGACCCTC TCCTTCGCTC CCCGGACACA CACTCTCAAG 
AGCTCTGGCC GCATCCGAGA TGTCTTCCTC TGCCGACATG AGAAGATCTT CCACCCCAGC 
AAGGGCTATG TATATGTGGT GAAGGTGATG CGAGAGAATG CTCACGAGGC GACATACATC 
CAGAGGACAT TCGAGGAGTT CCAGGAACTG CACAATAAGC TGCGCCTGCT CTTCCCTTCT 
TCCTTCCTGC CCAGCTTCCC TAGCCGGTTT GTGATTGGCC GCTCCCGGGG AGAGGCAGTG 
GCTGAGAGGA GGAAGGAGGA GTTAAATGGC TACATCTGGC ACCTGATTCA TGCAGCCCCT 
GAGGTGGCTG AGTGTGATCT GGTGTATACT TTCTTCCACC CCCTGCCCCG AGATGAGAAG 
GCTTCAGGCC CCAGCCCAGC TCCGAAGTCC TCAGATGGGA CGTGGGCCCG GCCGGTTGGG 
AAGGTCGGTG GGGAGGTGAA GCTGTCTATC TCCTACAAAA ACAACAAACT CTTCATCATG 
GTGATGCATA TTCGGGGCTT GCAACCGCTC CAGGATGGCA ATGACCCTGA CCCCTATGTA 
AAGATTTACC TCCTTCCTGA CCCTCAGAAA ACCACAAAGA GGAAAACCAA GGTGGCCCGG 
AAAACCTGCA ACCCTACCTA CAATGAGATG CTGGTATATG ATGGGATCCC CAAGGGAGAC 
CTGCAGCAGC GGGAGCTACA GTTGAGTGTG CTGAGTGAGC AAGGATTTTG GGAGAATCTG 
CTTCTCGGGG AGGTGCACAT CCGCCTTCGG GAGCTGGACC TGGCCCAGGA GAAGACCGGC 
TGGTTTGGAC TGGGTTCTCG AGGTCATGGA ACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pik3c2b ( XM_006529531.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529594.1 Mus musculus phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 beta (Pik3c2b), transcript variant X2, mRNA.

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CloneID	OMu02575
Clone ID Related Accession (Same CDS sequence)	XM_006529531.3 , XM_006529532.2 , NM_001099276.2 , XM_006529531.4
Accession Version	XM_006529531.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4899bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006529531.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTCCA CTCAGGGCAA TGGGGAGCAC TGGAAGTCCC TGGAGTCGGT GGGCATCAGC 
CGCAAAGAGC TGGCTATGGC CGAAGCCCTG CAGATGGAGT ATGACGCTCT TTCCCGGCTC 
CGACATCACA AGGAGGAGAG CAGGGCCAAG CAGAACACAG AACCCTCTCT TATCAGCTGG 
GATGAGCCAG CCTTGGACTT CTACAGCAAG CCAGCAGGCA GGCGGACCGA CCTCAAGCTC 
TTGCGTGGTC TTTCTGGCTC TGATCCCACC CTAAATTACA ACTCCATCTC CCCACCAGAG 
GGGCTCCCCA ACTCTACCTC CCAGGATCCA CAGCCTGGCC CAGACCCCTG GCCCAAGGGC 
TCCCTGTCTG GAGACTATCT CTATATTTTT GATGGCTCAG AGGGGAGATG CTCTTTGTCT 
CCAGGATCAG GTGACACAGA TGGCTCTTGC AAGAAACTAT CCCCACCTCC TCTCCCTCCT 
CGTGTCTCTA TCTGGGATGC CCCTCCCTTG CCTCCTAGAA AAGGATCACC CTCACCCTCC 
AAGATCTCTC AGCCTAATGA CATCAACAGT TTCTCTTCGG CAGAACAGCC ACCTGACAAA 
CTGCTAGTGG CCCAGGACCC AGAAGAGGGA GAGCTGCCTG ATGGCAGGGG ACAGGGACAC 
ACGCTGGGGT CTGTGGACTA CGATGGCATC AATGATGCAA TTACCAGGCT CAACTTGAAG 
TCCACCTACG ACTCAGAGAT ATCGTCTGAT GCTACCAGGG GCTGGAAGGA GGGCCGAGGG 
CCCCTGGACT TCAACAAGGA CACCTCTGGG AAACCTGTGG CCCGGAGCAA GACGATGCCC 
CCTCAGGTGC CCCCTCGAAC CTATACTTCT CGATATGCCA ACCGAAAAAA TGCAACACCT 
GGCAATAACC GACGGATTTC TGCTGCTCCG GTGGGCTCTC GGCCCCACAC TGTTGCCAAT 
GGCCACGAAT TGTTTGAGGT CTCAGAGGAG AGAGATGAGG AAGTTGCTGC GTTCTGCCAC 
ATGCTGGATA TCCTTCGAAC GGGCTCTGAT ATCCAGGACT ATTCCCTAAC TGGGTGTGTC 
TGGAGCACTG TCACCCCAAG TCCAGAACAC CTTGGGGATG AGGTCAACCT GAAGGTGACA 
GTGTTGTGTG ACAGTCTCCG GGAGCCACTC ACCTTTACCT GCAACTGTTC CTCCACCGTG 
GACCTGCTCA TCCACCAGAC CCTCTGCTAC ACTCACGATG AGCTGAGGGA AGTGGACGTG 
GGCGACTTTG TGCTGAAGCC CTGTGGGCTG GAGGAGTTCT TGCAAAATAA GCACGCCCTG 
GGAAGCCATG AGTATATCCA ATATTGCCGC AAGTTTGATA TCAGCATCCG GCTACAGCTG 
ATGGAGCAGA AGGCTATCCG GAGCGACCTG GCCCGGACGG TGAATGACGA CCAGAGCCCT 
TCCACCTTGA ACTACCTCAT CCATCTCCAA GAGAGGCCAG TCAAGCAGAC CATCAGCAGG 
CAGGCCTTGA GTCTTCTGTT TGACACTTAC CACAATGAGG TGGACGCCTT CCTGCTGGCT 
GATGGGGATT TCCCACTGAA GGCTGACAGG GTGGTCCAGT CTGTCAAGGC CATTTGCAAT 
GCTCTGGCTG CTGTGGAGAC TCCCGAGATC ACGAGCGCTC TCAACCAGCT GCCTCCCTGC 
CCCTCGCGCA TGCAGCCAAA AATCCAGAAG GATCCCAGCG TGTTGAGTGT GAGGGAAAAC 
CGAGAGAAGG TGGTAGAAGC CTTGACAGCG GCCATCTTGG ACTTGGTGGA GCTGTACTGC 
AGCACATTCA ATGCAGACTT CCAGACGGCG GTGCCTGGGA GCCGAAAGCA TGATTTGGTT 
CAAGAGGCCT GCCACTTCCC TGGGGCCCTG GCCTTCACTG TCTATGGCAC TCACCGTATT 
CCCATCATCT GGGCTACCAG CTATGAAGAT TTCTACCTCT CCTGCTCCCT CAGCCACGGT 
GGCAAGGAAC TTTGCAGCCC CCTGCAGACC CGGCGGGCTC ACTTCTCCAA GTACCTGTTC 
CACCTCATCA TCTGGGACCA GCAGATCTGC TTTCCAGTGC AGGTGAACAG GCTACCTCGG 
GAGACTCTGC TGTGTGCCAC ACTCTATGCC CTGCCCGTTC CCCCTCCTGG GGGTTCCTCT 
GAAGCGAATA AGCAGAAGCG GGTGCCTGAA GCTCTGGGTT GGGTCACTAC CCCTCTCTTC 
AACTTCAGGC AAGTCTTGAC CTGTGGTCGA AAGCTCCTGG GCTTGTGGCC AGCGACACAG 
GAAAACTCCG GTGCCCGCTG GAGTGCGCCT AATTTCCACC AGCCAGACAG TGTTATCCTG 
CAGATTGACT TCCCCAGCTC GGCCTTTGAC ATCAAGTTCA CCAGCCCCCC TGGAGACAAG 
TTCAGCCCCC GCTATGAGTT TGGCAGTCTC CGGGAGGAGG ACCAGCGCAA ACTTAAAGAC 
ATTACGCAGA AGGAGTCCCT ATACTGGCTC ACGGATGCTG ACAAGAAGCA GCTGTGGGAG 
AAACGCTATT ACTGCCACAC TGAGGTGAGC TCACTCCCCC TGGTTCTCGC CAGTGCACCC 
AGCTGGGAGT GGGCTTGCCT GCCGGACATC TATGCTCTCC TGCAACAGTG GACTCACATG 
AACCACCAAG ATGCCCTGGG ACTCCTGCAT GCCACCTTCC CGGACCAGGA GGTGCGGCGG 
ATGGCCGTGC AGTGGATCGG CTCCCTCTCA GATGCGGAGC TGCTGGACTA CCTGCCCCAG 
CTAGTCCAGG CCCTGAAGTA TGAGTGCTAC CTGGATAGCC CCTTGGTGCG CTTTCTCCTG 
AAACGAGCCA TCTCTGACCT GCGAGTGACC CACTACTTCT TCTGGTTACT GAAGGACAGC 
CTCAAGGACT CTCAGTTCAG TATCCGCTAC CAATACCTGC TAGCAGCCCT GCTGTGCTGC 
TGTGGCAAGG GACTGAGGGA GGAGTTTAAC CGCCAGTGCT GGCTTGTCAA CACCCTGGCC 
AAACTGGCCC AGCAGGTCCG AGAGGCCACC CCATCCGCCC GCCAGGGCAT CCTTCGTGTG 
GGCTTGGAGG AGGTGAAGCA GTTCTTTGCC CTCAATGGTT CGTGCCGTCT GCCGCTCAGT 
CCCAGTCTCC TGGTGAAGGG CATCGTGCCC AGGGACTGCT CCTACTTCAA TTCCAACGCA 
GTGCCCCTGA AGCTTGCCTT CCAAAATGTG GATCCGCTGG GCGAGAACAT CAGAGTCATC 
TTTAAGTGTG GGGATGATCT TCGTCAGGAC ATGCTCACCC TGCAGATGAT TCGCATCATG 
AGCAAGATCT GGGTACAGGA GGGGCTGGAC ATGCGCATGG TCATCTTCCG CTGCTTCTCC 
ACTGGCCGAG GAAAAGGGAT GGTAGAGATG ATACCCAATG CAGAGACACT GCGCAAGATC 
CAGGTGGAAC ATGGGGTGAC TGGCTCCTTC AAGGACCGTC CCCTGGCCGA CTGGCTACAA 
AAACACAACC CTGGGGAGGA TGAATATGAA AAGGCTGTGG AGAACTTCAT CTACTCTTGT 
GCTGGCTGCT GTGTAGCTAC ATACATCTTG GGCATCTGTG ACAGACACAA TGACAACATC 
ATGCTAAAGA CCACGGGCCA CATGTTCCAT ATAGATTTCG GCCGCTTCCT GGGTCACGCA 
CAGATGTTTG GTAACATCAA GAGGGACCGT GCCCCGTTTG TCTTCACCTC AGACATGGCG 
TATGTCATCA ATGGGGGTGA CAAGCCTTCC AGCCGCTTCC ATGATTTTGT TGACCTTTGC 
TGCCAAGCCT ACAACCTCAT TCGCAAGCAC ACCCACCTCT TCCTCAACCT TCTGGGCCTG 
ATGCTGTCCT GTGGAATCCC CGAGCTCTCA GACCTGGAGG ACCTCAAGTA TGTGTATGAT 
GCCCTGAGAC CTCAGGACAC AGAGGCCAAT GCCACCACCT ACTTCACAAG GTTGATTGAG 
TCCAGCCTGG GCAGCGTAGC CACGAAACTC AACTTTTTCA TCCATAACCT GGCTCAGATG 
AAGTTCACAG GCTCAGATGA CCGGCTGACC CTCTCCTTCG CTCCCCGGAC ACACACTCTC 
AAGAGCTCTG GCCGCATCCG AGATGTCTTC CTCTGCCGAC ATGAGAAGAT CTTCCACCCC 
AGCAAGGGCT ATGTATATGT GGTGAAGGTG ATGCGAGAGA ATGCTCACGA GGCGACATAC 
ATCCAGAGGA CATTCGAGGA GTTCCAGGAA CTGCACAATA AGCTGCGCCT GCTCTTCCCT 
TCTTCCTTCC TGCCCAGCTT CCCTAGCCGG TTTGTGATTG GCCGCTCCCG GGGAGAGGCA 
GTGGCTGAGA GGAGGAAGGA GGAGTTAAAT GGCTACATCT GGCACCTGAT TCATGCAGCC 
CCTGAGGTGG CTGAGTGTGA TCTGGTGTAT ACTTTCTTCC ACCCCCTGCC CCGAGATGAG 
AAGGCTTCAG GCCCCAGCCC AGCTCCGAAG TCCTCAGATG GGACGTGGGC CCGGCCGGTT 
GGGAAGGTCG GTGGGGAGGT GAAGCTGTCT ATCTCCTACA AAAACAACAA ACTCTTCATC 
ATGGTGATGC ATATTCGGGG CTTGCAACCG CTCCAGGATG GCAATGACCC TGACCCCTAT 
GTAAAGATTT ACCTCCTTCC TGACCCTCAG AAAACCACAA AGAGGAAAAC CAAGGTGGCC 
CGGAAAACCT GCAACCCTAC CTACAATGAG ATGCTGGTAT ATGATGGGAT CCCCAAGGGA 
GACCTGCAGC AGCGGGAGCT ACAGTTGAGT GTGCTGAGTG AGCAAGGATT TTGGGAGAAT 
CTGCTTCTCG GGGAGGTGCA CATCCGCCTT CGGGAGCTGG ACCTGGCCCA GGAGAAGACC 
GGCTGGTTTG GACTGGGTTC TCGAGGTCAT GGAACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529594.1
CDS	303..5201
Translation	MSSTQGNGEHWKSLESVGISRKELAMAEALQMEYDALSRLRHHKEESRAKQNTEPSLISWDEPALDFYSKPAGRRTDLKLLRGLSGSDPTLNYNSISPPEGLPNSTSQDPQPGPDPWPKGSLSGDYLYIFDGSEGRCSLSPGSGDTDGSCKKLSPPPLPPRVSIWDAPPLPPRKGSPSPSKISQPNDINSFSSAEQPPDKLLVAQDPEEGELPDGRGQGHTLGSVDYDGINDAITRLNLKSTYDSEISSDATRGWKEGRGPLDFNKDTSGKPVARSKTMPPQVPPRTYTSRYANRKNATPGNNRRISAAPVGSRPHTVANGHELFEVSEERDEEVAAFCHMLDILRTGSDIQDYSLTGCVWSTVTPSPEHLGDEVNLKVTVLCDSLREPLTFTCNCSSTVDLLIHQTLCYTHDELREVDVGDFVLKPCGLEEFLQNKHALGSHEYIQYCRKFDISIRLQLMEQKAIRSDLARTVNDDQSPSTLNYLIHLQERPVKQTISRQALSLLFDTYHNEVDAFLLADGDFPLKADRVVQSVKAICNALAAVETPEITSALNQLPPCPSRMQPKIQKDPSVLSVRENREKVVEALTAAILDLVELYCSTFNADFQTAVPGSRKHDLVQEACHFPGALAFTVYGTHRIPIIWATSYEDFYLSCSLSHGGKELCSPLQTRRAHFSKYLFHLIIWDQQICFPVQVNRLPRETLLCATLYALPVPPPGGSSEANKQKRVPEALGWVTTPLFNFRQVLTCGRKLLGLWPATQENSGARWSAPNFHQPDSVILQIDFPSSAFDIKFTSPPGDKFSPRYEFGSLREEDQRKLKDITQKESLYWLTDADKKQLWEKRYYCHTEVSSLPLVLASAPSWEWACLPDIYALLQQWTHMNHQDALGLLHATFPDQEVRRMAVQWIGSLSDAELLDYLPQLVQALKYECYLDSPLVRFLLKRAISDLRVTHYFFWLLKDSLKDSQFSIRYQYLLAALLCCCGKGLREEFNRQCWLVNTLAKLAQQVREATPSARQGILRVGLEEVKQFFALNGSCRLPLSPSLLVKGIVPRDCSYFNSNAVPLKLAFQNVDPLGENIRVIFKCGDDLRQDMLTLQMIRIMSKIWVQEGLDMRMVIFRCFSTGRGKGMVEMIPNAETLRKIQVEHGVTGSFKDRPLADWLQKHNPGEDEYEKAVENFIYSCAGCCVATYILGICDRHNDNIMLKTTGHMFHIDFGRFLGHAQMFGNIKRDRAPFVFTSDMAYVINGGDKPSSRFHDFVDLCCQAYNLIRKHTHLFLNLLGLMLSCGIPELSDLEDLKYVYDALRPQDTEANATTYFTRLIESSLGSVATKLNFFIHNLAQMKFTGSDDRLTLSFAPRTHTLKSSGRIRDVFLCRHEKIFHPSKGYVYVVKVMRENAHEATYIQRTFEEFQELHNKLRLLFPSSFLPSFPSRFVIGRSRGEAVAERRKEELNGYIWHLIHAAPEVAECDLVYTFFHPLPRDEKASGPSPAPKSSDGTWARPVGKVGGEVKLSISYKNNKLFIMVMHIRGLQPLQDGNDPDPYVKIYLLPDPQKTTKRKTKVARKTCNPTYNEMLVYDGIPKGDLQQRELQLSVLSEQGFWENLLLGEVHIRLRELDLAQEKTGWFGLGSRGHGT

Target ORF information:

RefSeq Version	XM_006529531.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 beta (Pik3c2b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529531.4

ORF Insert Sequence:

ATGTCTTCCA CTCAGGGCAA TGGGGAGCAC TGGAAGTCCC TGGAGTCGGT GGGCATCAGC 
CGCAAAGAGC TGGCTATGGC CGAAGCCCTG CAGATGGAGT ATGACGCTCT TTCCCGGCTC 
CGACATCACA AGGAGGAGAG CAGGGCCAAG CAGAACACAG AACCCTCTCT TATCAGCTGG 
GATGAGCCAG CCTTGGACTT CTACAGCAAG CCAGCAGGCA GGCGGACCGA CCTCAAGCTC 
TTGCGTGGTC TTTCTGGCTC TGATCCCACC CTAAATTACA ACTCCATCTC CCCACCAGAG 
GGGCTCCCCA ACTCTACCTC CCAGGATCCA CAGCCTGGCC CAGACCCCTG GCCCAAGGGC 
TCCCTGTCTG GAGACTATCT CTATATTTTT GATGGCTCAG AGGGGAGATG CTCTTTGTCT 
CCAGGATCAG GTGACACAGA TGGCTCTTGC AAGAAACTAT CCCCACCTCC TCTCCCTCCT 
CGTGTCTCTA TCTGGGATGC CCCTCCCTTG CCTCCTAGAA AAGGATCACC CTCACCCTCC 
AAGATCTCTC AGCCTAATGA CATCAACAGT TTCTCTTCGG CAGAACAGCC ACCTGACAAA 
CTGCTAGTGG CCCAGGACCC AGAAGAGGGA GAGCTGCCTG ATGGCAGGGG ACAGGGACAC 
ACGCTGGGGT CTGTGGACTA CGATGGCATC AATGATGCAA TTACCAGGCT CAACTTGAAG 
TCCACCTACG ACTCAGAGAT ATCGTCTGAT GCTACCAGGG GCTGGAAGGA GGGCCGAGGG 
CCCCTGGACT TCAACAAGGA CACCTCTGGG AAACCTGTGG CCCGGAGCAA GACGATGCCC 
CCTCAGGTGC CCCCTCGAAC CTATACTTCT CGATATGCCA ACCGAAAAAA TGCAACACCT 
GGCAATAACC GACGGATTTC TGCTGCTCCG GTGGGCTCTC GGCCCCACAC TGTTGCCAAT 
GGCCACGAAT TGTTTGAGGT CTCAGAGGAG AGAGATGAGG AAGTTGCTGC GTTCTGCCAC 
ATGCTGGATA TCCTTCGAAC GGGCTCTGAT ATCCAGGACT ATTCCCTAAC TGGGTGTGTC 
TGGAGCACTG TCACCCCAAG TCCAGAACAC CTTGGGGATG AGGTCAACCT GAAGGTGACA 
GTGTTGTGTG ACAGTCTCCG GGAGCCACTC ACCTTTACCT GCAACTGTTC CTCCACCGTG 
GACCTGCTCA TCCACCAGAC CCTCTGCTAC ACTCACGATG AGCTGAGGGA AGTGGACGTG 
GGCGACTTTG TGCTGAAGCC CTGTGGGCTG GAGGAGTTCT TGCAAAATAA GCACGCCCTG 
GGAAGCCATG AGTATATCCA ATATTGCCGC AAGTTTGATA TCAGCATCCG GCTACAGCTG 
ATGGAGCAGA AGGCTATCCG GAGCGACCTG GCCCGGACGG TGAATGACGA CCAGAGCCCT 
TCCACCTTGA ACTACCTCAT CCATCTCCAA GAGAGGCCAG TCAAGCAGAC CATCAGCAGG 
CAGGCCTTGA GTCTTCTGTT TGACACTTAC CACAATGAGG TGGACGCCTT CCTGCTGGCT 
GATGGGGATT TCCCACTGAA GGCTGACAGG GTGGTCCAGT CTGTCAAGGC CATTTGCAAT 
GCTCTGGCTG CTGTGGAGAC TCCCGAGATC ACGAGCGCTC TCAACCAGCT GCCTCCCTGC 
CCCTCGCGCA TGCAGCCAAA AATCCAGAAG GATCCCAGCG TGTTGAGTGT GAGGGAAAAC 
CGAGAGAAGG TGGTAGAAGC CTTGACAGCG GCCATCTTGG ACTTGGTGGA GCTGTACTGC 
AGCACATTCA ATGCAGACTT CCAGACGGCG GTGCCTGGGA GCCGAAAGCA TGATTTGGTT 
CAAGAGGCCT GCCACTTCCC TGGGGCCCTG GCCTTCACTG TCTATGGCAC TCACCGTATT 
CCCATCATCT GGGCTACCAG CTATGAAGAT TTCTACCTCT CCTGCTCCCT CAGCCACGGT 
GGCAAGGAAC TTTGCAGCCC CCTGCAGACC CGGCGGGCTC ACTTCTCCAA GTACCTGTTC 
CACCTCATCA TCTGGGACCA GCAGATCTGC TTTCCAGTGC AGGTGAACAG GCTACCTCGG 
GAGACTCTGC TGTGTGCCAC ACTCTATGCC CTGCCCGTTC CCCCTCCTGG GGGTTCCTCT 
GAAGCGAATA AGCAGAAGCG GGTGCCTGAA GCTCTGGGTT GGGTCACTAC CCCTCTCTTC 
AACTTCAGGC AAGTCTTGAC CTGTGGTCGA AAGCTCCTGG GCTTGTGGCC AGCGACACAG 
GAAAACTCCG GTGCCCGCTG GAGTGCGCCT AATTTCCACC AGCCAGACAG TGTTATCCTG 
CAGATTGACT TCCCCAGCTC GGCCTTTGAC ATCAAGTTCA CCAGCCCCCC TGGAGACAAG 
TTCAGCCCCC GCTATGAGTT TGGCAGTCTC CGGGAGGAGG ACCAGCGCAA ACTTAAAGAC 
ATTACGCAGA AGGAGTCCCT ATACTGGCTC ACGGATGCTG ACAAGAAGCA GCTGTGGGAG 
AAACGCTATT ACTGCCACAC TGAGGTGAGC TCACTCCCCC TGGTTCTCGC CAGTGCACCC 
AGCTGGGAGT GGGCTTGCCT GCCGGACATC TATGCTCTCC TGCAACAGTG GACTCACATG 
AACCACCAAG ATGCCCTGGG ACTCCTGCAT GCCACCTTCC CGGACCAGGA GGTGCGGCGG 
ATGGCCGTGC AGTGGATCGG CTCCCTCTCA GATGCGGAGC TGCTGGACTA CCTGCCCCAG 
CTAGTCCAGG CCCTGAAGTA TGAGTGCTAC CTGGATAGCC CCTTGGTGCG CTTTCTCCTG 
AAACGAGCCA TCTCTGACCT GCGAGTGACC CACTACTTCT TCTGGTTACT GAAGGACAGC 
CTCAAGGACT CTCAGTTCAG TATCCGCTAC CAATACCTGC TAGCAGCCCT GCTGTGCTGC 
TGTGGCAAGG GACTGAGGGA GGAGTTTAAC CGCCAGTGCT GGCTTGTCAA CACCCTGGCC 
AAACTGGCCC AGCAGGTCCG AGAGGCCACC CCATCCGCCC GCCAGGGCAT CCTTCGTGTG 
GGCTTGGAGG AGGTGAAGCA GTTCTTTGCC CTCAATGGTT CGTGCCGTCT GCCGCTCAGT 
CCCAGTCTCC TGGTGAAGGG CATCGTGCCC AGGGACTGCT CCTACTTCAA TTCCAACGCA 
GTGCCCCTGA AGCTTGCCTT CCAAAATGTG GATCCGCTGG GCGAGAACAT CAGAGTCATC 
TTTAAGTGTG GGGATGATCT TCGTCAGGAC ATGCTCACCC TGCAGATGAT TCGCATCATG 
AGCAAGATCT GGGTACAGGA GGGGCTGGAC ATGCGCATGG TCATCTTCCG CTGCTTCTCC 
ACTGGCCGAG GAAAAGGGAT GGTAGAGATG ATACCCAATG CAGAGACACT GCGCAAGATC 
CAGGTGGAAC ATGGGGTGAC TGGCTCCTTC AAGGACCGTC CCCTGGCCGA CTGGCTACAA 
AAACACAACC CTGGGGAGGA TGAATATGAA AAGGCTGTGG AGAACTTCAT CTACTCTTGT 
GCTGGCTGCT GTGTAGCTAC ATACATCTTG GGCATCTGTG ACAGACACAA TGACAACATC 
ATGCTAAAGA CCACGGGCCA CATGTTCCAT ATAGATTTCG GCCGCTTCCT GGGTCACGCA 
CAGATGTTTG GTAACATCAA GAGGGACCGT GCCCCGTTTG TCTTCACCTC AGACATGGCG 
TATGTCATCA ATGGGGGTGA CAAGCCTTCC AGCCGCTTCC ATGATTTTGT TGACCTTTGC 
TGCCAAGCCT ACAACCTCAT TCGCAAGCAC ACCCACCTCT TCCTCAACCT TCTGGGCCTG 
ATGCTGTCCT GTGGAATCCC CGAGCTCTCA GACCTGGAGG ACCTCAAGTA TGTGTATGAT 
GCCCTGAGAC CTCAGGACAC AGAGGCCAAT GCCACCACCT ACTTCACAAG GTTGATTGAG 
TCCAGCCTGG GCAGCGTAGC CACGAAACTC AACTTTTTCA TCCATAACCT GGCTCAGATG 
AAGTTCACAG GCTCAGATGA CCGGCTGACC CTCTCCTTCG CTCCCCGGAC ACACACTCTC 
AAGAGCTCTG GCCGCATCCG AGATGTCTTC CTCTGCCGAC ATGAGAAGAT CTTCCACCCC 
AGCAAGGGCT ATGTATATGT GGTGAAGGTG ATGCGAGAGA ATGCTCACGA GGCGACATAC 
ATCCAGAGGA CATTCGAGGA GTTCCAGGAA CTGCACAATA AGCTGCGCCT GCTCTTCCCT 
TCTTCCTTCC TGCCCAGCTT CCCTAGCCGG TTTGTGATTG GCCGCTCCCG GGGAGAGGCA 
GTGGCTGAGA GGAGGAAGGA GGAGTTAAAT GGCTACATCT GGCACCTGAT TCATGCAGCC 
CCTGAGGTGG CTGAGTGTGA TCTGGTGTAT ACTTTCTTCC ACCCCCTGCC CCGAGATGAG 
AAGGCTTCAG GCCCCAGCCC AGCTCCGAAG TCCTCAGATG GGACGTGGGC CCGGCCGGTT 
GGGAAGGTCG GTGGGGAGGT GAAGCTGTCT ATCTCCTACA AAAACAACAA ACTCTTCATC 
ATGGTGATGC ATATTCGGGG CTTGCAACCG CTCCAGGATG GCAATGACCC TGACCCCTAT 
GTAAAGATTT ACCTCCTTCC TGACCCTCAG AAAACCACAA AGAGGAAAAC CAAGGTGGCC 
CGGAAAACCT GCAACCCTAC CTACAATGAG ATGCTGGTAT ATGATGGGAT CCCCAAGGGA 
GACCTGCAGC AGCGGGAGCT ACAGTTGAGT GTGCTGAGTG AGCAAGGATT TTGGGAGAAT 
CTGCTTCTCG GGGAGGTGCA CATCCGCCTT CGGGAGCTGG ACCTGGCCCA GGAGAAGACC 
GGCTGGTTTG GACTGGGTTC TCGAGGTCAT GGAACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pik3c2b ( NM_001099276.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001092746.2 Mus musculus phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 beta (Pik3c2b), mRNA.

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CloneID	OMu02575
Clone ID Related Accession (Same CDS sequence)	XM_006529531.3 , XM_006529532.2 , NM_001099276.2 , XM_006529531.4
Accession Version	NM_001099276.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4899bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-26
Organism	Mus musculus(house mouse)
Product	phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit beta
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC137948.12 and AC107842.13. On or before Sep 19, 2009 this sequence version replaced XM_914845.2, XM_001479658.1, NM_001099276.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. The combination of the 5' non-coding exon and alternatively spliced exons within the coding region is inferred based on available transcript data and further supported by similar exon-intron structure of the related human gene, GeneID 5287. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTTCCA CTCAGGGCAA TGGGGAGCAC TGGAAGTCCC TGGAGTCGGT GGGCATCAGC 
CGCAAAGAGC TGGCTATGGC CGAAGCCCTG CAGATGGAGT ATGACGCTCT TTCCCGGCTC 
CGACATCACA AGGAGGAGAG CAGGGCCAAG CAGAACACAG AACCCTCTCT TATCAGCTGG 
GATGAGCCAG CCTTGGACTT CTACAGCAAG CCAGCAGGCA GGCGGACCGA CCTCAAGCTC 
TTGCGTGGTC TTTCTGGCTC TGATCCCACC CTAAATTACA ACTCCATCTC CCCACCAGAG 
GGGCTCCCCA ACTCTACCTC CCAGGATCCA CAGCCTGGCC CAGACCCCTG GCCCAAGGGC 
TCCCTGTCTG GAGACTATCT CTATATTTTT GATGGCTCAG AGGGGAGATG CTCTTTGTCT 
CCAGGATCAG GTGACACAGA TGGCTCTTGC AAGAAACTAT CCCCACCTCC TCTCCCTCCT 
CGTGTCTCTA TCTGGGATGC CCCTCCCTTG CCTCCTAGAA AAGGATCACC CTCACCCTCC 
AAGATCTCTC AGCCTAATGA CATCAACAGT TTCTCTTCGG CAGAACAGCC ACCTGACAAA 
CTGCTAGTGG CCCAGGACCC AGAAGAGGGA GAGCTGCCTG ATGGCAGGGG ACAGGGACAC 
ACGCTGGGGT CTGTGGACTA CGATGGCATC AATGATGCAA TTACCAGGCT CAACTTGAAG 
TCCACCTACG ACTCAGAGAT ATCGTCTGAT GCTACCAGGG GCTGGAAGGA GGGCCGAGGG 
CCCCTGGACT TCAACAAGGA CACCTCTGGG AAACCTGTGG CCCGGAGCAA GACGATGCCC 
CCTCAGGTGC CCCCTCGAAC CTATACTTCT CGATATGCCA ACCGAAAAAA TGCAACACCT 
GGCAATAACC GACGGATTTC TGCTGCTCCG GTGGGCTCTC GGCCCCACAC TGTTGCCAAT 
GGCCACGAAT TGTTTGAGGT CTCAGAGGAG AGAGATGAGG AAGTTGCTGC GTTCTGCCAC 
ATGCTGGATA TCCTTCGAAC GGGCTCTGAT ATCCAGGACT ATTCCCTAAC TGGGTGTGTC 
TGGAGCACTG TCACCCCAAG TCCAGAACAC CTTGGGGATG AGGTCAACCT GAAGGTGACA 
GTGTTGTGTG ACAGTCTCCG GGAGCCACTC ACCTTTACCT GCAACTGTTC CTCCACCGTG 
GACCTGCTCA TCCACCAGAC CCTCTGCTAC ACTCACGATG AGCTGAGGGA AGTGGACGTG 
GGCGACTTTG TGCTGAAGCC CTGTGGGCTG GAGGAGTTCT TGCAAAATAA GCACGCCCTG 
GGAAGCCATG AGTATATCCA ATATTGCCGC AAGTTTGATA TCAGCATCCG GCTACAGCTG 
ATGGAGCAGA AGGCTATCCG GAGCGACCTG GCCCGGACGG TGAATGACGA CCAGAGCCCT 
TCCACCTTGA ACTACCTCAT CCATCTCCAA GAGAGGCCAG TCAAGCAGAC CATCAGCAGG 
CAGGCCTTGA GTCTTCTGTT TGACACTTAC CACAATGAGG TGGACGCCTT CCTGCTGGCT 
GATGGGGATT TCCCACTGAA GGCTGACAGG GTGGTCCAGT CTGTCAAGGC CATTTGCAAT 
GCTCTGGCTG CTGTGGAGAC TCCCGAGATC ACGAGCGCTC TCAACCAGCT GCCTCCCTGC 
CCCTCGCGCA TGCAGCCAAA AATCCAGAAG GATCCCAGCG TGTTGAGTGT GAGGGAAAAC 
CGAGAGAAGG TGGTAGAAGC CTTGACAGCG GCCATCTTGG ACTTGGTGGA GCTGTACTGC 
AGCACATTCA ATGCAGACTT CCAGACGGCG GTGCCTGGGA GCCGAAAGCA TGATTTGGTT 
CAAGAGGCCT GCCACTTCCC TGGGGCCCTG GCCTTCACTG TCTATGGCAC TCACCGTATT 
CCCATCATCT GGGCTACCAG CTATGAAGAT TTCTACCTCT CCTGCTCCCT CAGCCACGGT 
GGCAAGGAAC TTTGCAGCCC CCTGCAGACC CGGCGGGCTC ACTTCTCCAA GTACCTGTTC 
CACCTCATCA TCTGGGACCA GCAGATCTGC TTTCCAGTGC AGGTGAACAG GCTACCTCGG 
GAGACTCTGC TGTGTGCCAC ACTCTATGCC CTGCCCGTTC CCCCTCCTGG GGGTTCCTCT 
GAAGCGAATA AGCAGAAGCG GGTGCCTGAA GCTCTGGGTT GGGTCACTAC CCCTCTCTTC 
AACTTCAGGC AAGTCTTGAC CTGTGGTCGA AAGCTCCTGG GCTTGTGGCC AGCGACACAG 
GAAAACTCCG GTGCCCGCTG GAGTGCGCCT AATTTCCACC AGCCAGACAG TGTTATCCTG 
CAGATTGACT TCCCCAGCTC GGCCTTTGAC ATCAAGTTCA CCAGCCCCCC TGGAGACAAG 
TTCAGCCCCC GCTATGAGTT TGGCAGTCTC CGGGAGGAGG ACCAGCGCAA ACTTAAAGAC 
ATTACGCAGA AGGAGTCCCT ATACTGGCTC ACGGATGCTG ACAAGAAGCA GCTGTGGGAG 
AAACGCTATT ACTGCCACAC TGAGGTGAGC TCACTCCCCC TGGTTCTCGC CAGTGCACCC 
AGCTGGGAGT GGGCTTGCCT GCCGGACATC TATGCTCTCC TGCAACAGTG GACTCACATG 
AACCACCAAG ATGCCCTGGG ACTCCTGCAT GCCACCTTCC CGGACCAGGA GGTGCGGCGG 
ATGGCCGTGC AGTGGATCGG CTCCCTCTCA GATGCGGAGC TGCTGGACTA CCTGCCCCAG 
CTAGTCCAGG CCCTGAAGTA TGAGTGCTAC CTGGATAGCC CCTTGGTGCG CTTTCTCCTG 
AAACGAGCCA TCTCTGACCT GCGAGTGACC CACTACTTCT TCTGGTTACT GAAGGACAGC 
CTCAAGGACT CTCAGTTCAG TATCCGCTAC CAATACCTGC TAGCAGCCCT GCTGTGCTGC 
TGTGGCAAGG GACTGAGGGA GGAGTTTAAC CGCCAGTGCT GGCTTGTCAA CACCCTGGCC 
AAACTGGCCC AGCAGGTCCG AGAGGCCACC CCATCCGCCC GCCAGGGCAT CCTTCGTGTG 
GGCTTGGAGG AGGTGAAGCA GTTCTTTGCC CTCAATGGTT CGTGCCGTCT GCCGCTCAGT 
CCCAGTCTCC TGGTGAAGGG CATCGTGCCC AGGGACTGCT CCTACTTCAA TTCCAACGCA 
GTGCCCCTGA AGCTTGCCTT CCAAAATGTG GATCCGCTGG GCGAGAACAT CAGAGTCATC 
TTTAAGTGTG GGGATGATCT TCGTCAGGAC ATGCTCACCC TGCAGATGAT TCGCATCATG 
AGCAAGATCT GGGTACAGGA GGGGCTGGAC ATGCGCATGG TCATCTTCCG CTGCTTCTCC 
ACTGGCCGAG GAAAAGGGAT GGTAGAGATG ATACCCAATG CAGAGACACT GCGCAAGATC 
CAGGTGGAAC ATGGGGTGAC TGGCTCCTTC AAGGACCGTC CCCTGGCCGA CTGGCTACAA 
AAACACAACC CTGGGGAGGA TGAATATGAA AAGGCTGTGG AGAACTTCAT CTACTCTTGT 
GCTGGCTGCT GTGTAGCTAC ATACATCTTG GGCATCTGTG ACAGACACAA TGACAACATC 
ATGCTAAAGA CCACGGGCCA CATGTTCCAT ATAGATTTCG GCCGCTTCCT GGGTCACGCA 
CAGATGTTTG GTAACATCAA GAGGGACCGT GCCCCGTTTG TCTTCACCTC AGACATGGCG 
TATGTCATCA ATGGGGGTGA CAAGCCTTCC AGCCGCTTCC ATGATTTTGT TGACCTTTGC 
TGCCAAGCCT ACAACCTCAT TCGCAAGCAC ACCCACCTCT TCCTCAACCT TCTGGGCCTG 
ATGCTGTCCT GTGGAATCCC CGAGCTCTCA GACCTGGAGG ACCTCAAGTA TGTGTATGAT 
GCCCTGAGAC CTCAGGACAC AGAGGCCAAT GCCACCACCT ACTTCACAAG GTTGATTGAG 
TCCAGCCTGG GCAGCGTAGC CACGAAACTC AACTTTTTCA TCCATAACCT GGCTCAGATG 
AAGTTCACAG GCTCAGATGA CCGGCTGACC CTCTCCTTCG CTCCCCGGAC ACACACTCTC 
AAGAGCTCTG GCCGCATCCG AGATGTCTTC CTCTGCCGAC ATGAGAAGAT CTTCCACCCC 
AGCAAGGGCT ATGTATATGT GGTGAAGGTG ATGCGAGAGA ATGCTCACGA GGCGACATAC 
ATCCAGAGGA CATTCGAGGA GTTCCAGGAA CTGCACAATA AGCTGCGCCT GCTCTTCCCT 
TCTTCCTTCC TGCCCAGCTT CCCTAGCCGG TTTGTGATTG GCCGCTCCCG GGGAGAGGCA 
GTGGCTGAGA GGAGGAAGGA GGAGTTAAAT GGCTACATCT GGCACCTGAT TCATGCAGCC 
CCTGAGGTGG CTGAGTGTGA TCTGGTGTAT ACTTTCTTCC ACCCCCTGCC CCGAGATGAG 
AAGGCTTCAG GCCCCAGCCC AGCTCCGAAG TCCTCAGATG GGACGTGGGC CCGGCCGGTT 
GGGAAGGTCG GTGGGGAGGT GAAGCTGTCT ATCTCCTACA AAAACAACAA ACTCTTCATC 
ATGGTGATGC ATATTCGGGG CTTGCAACCG CTCCAGGATG GCAATGACCC TGACCCCTAT 
GTAAAGATTT ACCTCCTTCC TGACCCTCAG AAAACCACAA AGAGGAAAAC CAAGGTGGCC 
CGGAAAACCT GCAACCCTAC CTACAATGAG ATGCTGGTAT ATGATGGGAT CCCCAAGGGA 
GACCTGCAGC AGCGGGAGCT ACAGTTGAGT GTGCTGAGTG AGCAAGGATT TTGGGAGAAT 
CTGCTTCTCG GGGAGGTGCA CATCCGCCTT CGGGAGCTGG ACCTGGCCCA GGAGAAGACC 
GGCTGGTTTG GACTGGGTTC TCGAGGTCAT GGAACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001092746.2
CDS	501..5399
Translation	MSSTQGNGEHWKSLESVGISRKELAMAEALQMEYDALSRLRHHKEESRAKQNTEPSLISWDEPALDFYSKPAGRRTDLKLLRGLSGSDPTLNYNSISPPEGLPNSTSQDPQPGPDPWPKGSLSGDYLYIFDGSEGRCSLSPGSGDTDGSCKKLSPPPLPPRVSIWDAPPLPPRKGSPSPSKISQPNDINSFSSAEQPPDKLLVAQDPEEGELPDGRGQGHTLGSVDYDGINDAITRLNLKSTYDSEISSDATRGWKEGRGPLDFNKDTSGKPVARSKTMPPQVPPRTYTSRYANRKNATPGNNRRISAAPVGSRPHTVANGHELFEVSEERDEEVAAFCHMLDILRTGSDIQDYSLTGCVWSTVTPSPEHLGDEVNLKVTVLCDSLREPLTFTCNCSSTVDLLIHQTLCYTHDELREVDVGDFVLKPCGLEEFLQNKHALGSHEYIQYCRKFDISIRLQLMEQKAIRSDLARTVNDDQSPSTLNYLIHLQERPVKQTISRQALSLLFDTYHNEVDAFLLADGDFPLKADRVVQSVKAICNALAAVETPEITSALNQLPPCPSRMQPKIQKDPSVLSVRENREKVVEALTAAILDLVELYCSTFNADFQTAVPGSRKHDLVQEACHFPGALAFTVYGTHRIPIIWATSYEDFYLSCSLSHGGKELCSPLQTRRAHFSKYLFHLIIWDQQICFPVQVNRLPRETLLCATLYALPVPPPGGSSEANKQKRVPEALGWVTTPLFNFRQVLTCGRKLLGLWPATQENSGARWSAPNFHQPDSVILQIDFPSSAFDIKFTSPPGDKFSPRYEFGSLREEDQRKLKDITQKESLYWLTDADKKQLWEKRYYCHTEVSSLPLVLASAPSWEWACLPDIYALLQQWTHMNHQDALGLLHATFPDQEVRRMAVQWIGSLSDAELLDYLPQLVQALKYECYLDSPLVRFLLKRAISDLRVTHYFFWLLKDSLKDSQFSIRYQYLLAALLCCCGKGLREEFNRQCWLVNTLAKLAQQVREATPSARQGILRVGLEEVKQFFALNGSCRLPLSPSLLVKGIVPRDCSYFNSNAVPLKLAFQNVDPLGENIRVIFKCGDDLRQDMLTLQMIRIMSKIWVQEGLDMRMVIFRCFSTGRGKGMVEMIPNAETLRKIQVEHGVTGSFKDRPLADWLQKHNPGEDEYEKAVENFIYSCAGCCVATYILGICDRHNDNIMLKTTGHMFHIDFGRFLGHAQMFGNIKRDRAPFVFTSDMAYVINGGDKPSSRFHDFVDLCCQAYNLIRKHTHLFLNLLGLMLSCGIPELSDLEDLKYVYDALRPQDTEANATTYFTRLIESSLGSVATKLNFFIHNLAQMKFTGSDDRLTLSFAPRTHTLKSSGRIRDVFLCRHEKIFHPSKGYVYVVKVMRENAHEATYIQRTFEEFQELHNKLRLLFPSSFLPSFPSRFVIGRSRGEAVAERRKEELNGYIWHLIHAAPEVAECDLVYTFFHPLPRDEKASGPSPAPKSSDGTWARPVGKVGGEVKLSISYKNNKLFIMVMHIRGLQPLQDGNDPDPYVKIYLLPDPQKTTKRKTKVARKTCNPTYNEMLVYDGIPKGDLQQRELQLSVLSEQGFWENLLLGEVHIRLRELDLAQEKTGWFGLGSRGHGT

Target ORF information:

RefSeq Version	NM_001099276.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 beta (Pik3c2b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001099276.2

ORF Insert Sequence:

ATGTCTTCCA CTCAGGGCAA TGGGGAGCAC TGGAAGTCCC TGGAGTCGGT GGGCATCAGC 
CGCAAAGAGC TGGCTATGGC CGAAGCCCTG CAGATGGAGT ATGACGCTCT TTCCCGGCTC 
CGACATCACA AGGAGGAGAG CAGGGCCAAG CAGAACACAG AACCCTCTCT TATCAGCTGG 
GATGAGCCAG CCTTGGACTT CTACAGCAAG CCAGCAGGCA GGCGGACCGA CCTCAAGCTC 
TTGCGTGGTC TTTCTGGCTC TGATCCCACC CTAAATTACA ACTCCATCTC CCCACCAGAG 
GGGCTCCCCA ACTCTACCTC CCAGGATCCA CAGCCTGGCC CAGACCCCTG GCCCAAGGGC 
TCCCTGTCTG GAGACTATCT CTATATTTTT GATGGCTCAG AGGGGAGATG CTCTTTGTCT 
CCAGGATCAG GTGACACAGA TGGCTCTTGC AAGAAACTAT CCCCACCTCC TCTCCCTCCT 
CGTGTCTCTA TCTGGGATGC CCCTCCCTTG CCTCCTAGAA AAGGATCACC CTCACCCTCC 
AAGATCTCTC AGCCTAATGA CATCAACAGT TTCTCTTCGG CAGAACAGCC ACCTGACAAA 
CTGCTAGTGG CCCAGGACCC AGAAGAGGGA GAGCTGCCTG ATGGCAGGGG ACAGGGACAC 
ACGCTGGGGT CTGTGGACTA CGATGGCATC AATGATGCAA TTACCAGGCT CAACTTGAAG 
TCCACCTACG ACTCAGAGAT ATCGTCTGAT GCTACCAGGG GCTGGAAGGA GGGCCGAGGG 
CCCCTGGACT TCAACAAGGA CACCTCTGGG AAACCTGTGG CCCGGAGCAA GACGATGCCC 
CCTCAGGTGC CCCCTCGAAC CTATACTTCT CGATATGCCA ACCGAAAAAA TGCAACACCT 
GGCAATAACC GACGGATTTC TGCTGCTCCG GTGGGCTCTC GGCCCCACAC TGTTGCCAAT 
GGCCACGAAT TGTTTGAGGT CTCAGAGGAG AGAGATGAGG AAGTTGCTGC GTTCTGCCAC 
ATGCTGGATA TCCTTCGAAC GGGCTCTGAT ATCCAGGACT ATTCCCTAAC TGGGTGTGTC 
TGGAGCACTG TCACCCCAAG TCCAGAACAC CTTGGGGATG AGGTCAACCT GAAGGTGACA 
GTGTTGTGTG ACAGTCTCCG GGAGCCACTC ACCTTTACCT GCAACTGTTC CTCCACCGTG 
GACCTGCTCA TCCACCAGAC CCTCTGCTAC ACTCACGATG AGCTGAGGGA AGTGGACGTG 
GGCGACTTTG TGCTGAAGCC CTGTGGGCTG GAGGAGTTCT TGCAAAATAA GCACGCCCTG 
GGAAGCCATG AGTATATCCA ATATTGCCGC AAGTTTGATA TCAGCATCCG GCTACAGCTG 
ATGGAGCAGA AGGCTATCCG GAGCGACCTG GCCCGGACGG TGAATGACGA CCAGAGCCCT 
TCCACCTTGA ACTACCTCAT CCATCTCCAA GAGAGGCCAG TCAAGCAGAC CATCAGCAGG 
CAGGCCTTGA GTCTTCTGTT TGACACTTAC CACAATGAGG TGGACGCCTT CCTGCTGGCT 
GATGGGGATT TCCCACTGAA GGCTGACAGG GTGGTCCAGT CTGTCAAGGC CATTTGCAAT 
GCTCTGGCTG CTGTGGAGAC TCCCGAGATC ACGAGCGCTC TCAACCAGCT GCCTCCCTGC 
CCCTCGCGCA TGCAGCCAAA AATCCAGAAG GATCCCAGCG TGTTGAGTGT GAGGGAAAAC 
CGAGAGAAGG TGGTAGAAGC CTTGACAGCG GCCATCTTGG ACTTGGTGGA GCTGTACTGC 
AGCACATTCA ATGCAGACTT CCAGACGGCG GTGCCTGGGA GCCGAAAGCA TGATTTGGTT 
CAAGAGGCCT GCCACTTCCC TGGGGCCCTG GCCTTCACTG TCTATGGCAC TCACCGTATT 
CCCATCATCT GGGCTACCAG CTATGAAGAT TTCTACCTCT CCTGCTCCCT CAGCCACGGT 
GGCAAGGAAC TTTGCAGCCC CCTGCAGACC CGGCGGGCTC ACTTCTCCAA GTACCTGTTC 
CACCTCATCA TCTGGGACCA GCAGATCTGC TTTCCAGTGC AGGTGAACAG GCTACCTCGG 
GAGACTCTGC TGTGTGCCAC ACTCTATGCC CTGCCCGTTC CCCCTCCTGG GGGTTCCTCT 
GAAGCGAATA AGCAGAAGCG GGTGCCTGAA GCTCTGGGTT GGGTCACTAC CCCTCTCTTC 
AACTTCAGGC AAGTCTTGAC CTGTGGTCGA AAGCTCCTGG GCTTGTGGCC AGCGACACAG 
GAAAACTCCG GTGCCCGCTG GAGTGCGCCT AATTTCCACC AGCCAGACAG TGTTATCCTG 
CAGATTGACT TCCCCAGCTC GGCCTTTGAC ATCAAGTTCA CCAGCCCCCC TGGAGACAAG 
TTCAGCCCCC GCTATGAGTT TGGCAGTCTC CGGGAGGAGG ACCAGCGCAA ACTTAAAGAC 
ATTACGCAGA AGGAGTCCCT ATACTGGCTC ACGGATGCTG ACAAGAAGCA GCTGTGGGAG 
AAACGCTATT ACTGCCACAC TGAGGTGAGC TCACTCCCCC TGGTTCTCGC CAGTGCACCC 
AGCTGGGAGT GGGCTTGCCT GCCGGACATC TATGCTCTCC TGCAACAGTG GACTCACATG 
AACCACCAAG ATGCCCTGGG ACTCCTGCAT GCCACCTTCC CGGACCAGGA GGTGCGGCGG 
ATGGCCGTGC AGTGGATCGG CTCCCTCTCA GATGCGGAGC TGCTGGACTA CCTGCCCCAG 
CTAGTCCAGG CCCTGAAGTA TGAGTGCTAC CTGGATAGCC CCTTGGTGCG CTTTCTCCTG 
AAACGAGCCA TCTCTGACCT GCGAGTGACC CACTACTTCT TCTGGTTACT GAAGGACAGC 
CTCAAGGACT CTCAGTTCAG TATCCGCTAC CAATACCTGC TAGCAGCCCT GCTGTGCTGC 
TGTGGCAAGG GACTGAGGGA GGAGTTTAAC CGCCAGTGCT GGCTTGTCAA CACCCTGGCC 
AAACTGGCCC AGCAGGTCCG AGAGGCCACC CCATCCGCCC GCCAGGGCAT CCTTCGTGTG 
GGCTTGGAGG AGGTGAAGCA GTTCTTTGCC CTCAATGGTT CGTGCCGTCT GCCGCTCAGT 
CCCAGTCTCC TGGTGAAGGG CATCGTGCCC AGGGACTGCT CCTACTTCAA TTCCAACGCA 
GTGCCCCTGA AGCTTGCCTT CCAAAATGTG GATCCGCTGG GCGAGAACAT CAGAGTCATC 
TTTAAGTGTG GGGATGATCT TCGTCAGGAC ATGCTCACCC TGCAGATGAT TCGCATCATG 
AGCAAGATCT GGGTACAGGA GGGGCTGGAC ATGCGCATGG TCATCTTCCG CTGCTTCTCC 
ACTGGCCGAG GAAAAGGGAT GGTAGAGATG ATACCCAATG CAGAGACACT GCGCAAGATC 
CAGGTGGAAC ATGGGGTGAC TGGCTCCTTC AAGGACCGTC CCCTGGCCGA CTGGCTACAA 
AAACACAACC CTGGGGAGGA TGAATATGAA AAGGCTGTGG AGAACTTCAT CTACTCTTGT 
GCTGGCTGCT GTGTAGCTAC ATACATCTTG GGCATCTGTG ACAGACACAA TGACAACATC 
ATGCTAAAGA CCACGGGCCA CATGTTCCAT ATAGATTTCG GCCGCTTCCT GGGTCACGCA 
CAGATGTTTG GTAACATCAA GAGGGACCGT GCCCCGTTTG TCTTCACCTC AGACATGGCG 
TATGTCATCA ATGGGGGTGA CAAGCCTTCC AGCCGCTTCC ATGATTTTGT TGACCTTTGC 
TGCCAAGCCT ACAACCTCAT TCGCAAGCAC ACCCACCTCT TCCTCAACCT TCTGGGCCTG 
ATGCTGTCCT GTGGAATCCC CGAGCTCTCA GACCTGGAGG ACCTCAAGTA TGTGTATGAT 
GCCCTGAGAC CTCAGGACAC AGAGGCCAAT GCCACCACCT ACTTCACAAG GTTGATTGAG 
TCCAGCCTGG GCAGCGTAGC CACGAAACTC AACTTTTTCA TCCATAACCT GGCTCAGATG 
AAGTTCACAG GCTCAGATGA CCGGCTGACC CTCTCCTTCG CTCCCCGGAC ACACACTCTC 
AAGAGCTCTG GCCGCATCCG AGATGTCTTC CTCTGCCGAC ATGAGAAGAT CTTCCACCCC 
AGCAAGGGCT ATGTATATGT GGTGAAGGTG ATGCGAGAGA ATGCTCACGA GGCGACATAC 
ATCCAGAGGA CATTCGAGGA GTTCCAGGAA CTGCACAATA AGCTGCGCCT GCTCTTCCCT 
TCTTCCTTCC TGCCCAGCTT CCCTAGCCGG TTTGTGATTG GCCGCTCCCG GGGAGAGGCA 
GTGGCTGAGA GGAGGAAGGA GGAGTTAAAT GGCTACATCT GGCACCTGAT TCATGCAGCC 
CCTGAGGTGG CTGAGTGTGA TCTGGTGTAT ACTTTCTTCC ACCCCCTGCC CCGAGATGAG 
AAGGCTTCAG GCCCCAGCCC AGCTCCGAAG TCCTCAGATG GGACGTGGGC CCGGCCGGTT 
GGGAAGGTCG GTGGGGAGGT GAAGCTGTCT ATCTCCTACA AAAACAACAA ACTCTTCATC 
ATGGTGATGC ATATTCGGGG CTTGCAACCG CTCCAGGATG GCAATGACCC TGACCCCTAT 
GTAAAGATTT ACCTCCTTCC TGACCCTCAG AAAACCACAA AGAGGAAAAC CAAGGTGGCC 
CGGAAAACCT GCAACCCTAC CTACAATGAG ATGCTGGTAT ATGATGGGAT CCCCAAGGGA 
GACCTGCAGC AGCGGGAGCT ACAGTTGAGT GTGCTGAGTG AGCAAGGATT TTGGGAGAAT 
CTGCTTCTCG GGGAGGTGCA CATCCGCCTT CGGGAGCTGG ACCTGGCCCA GGAGAAGACC 
GGCTGGTTTG GACTGGGTTC TCGAGGTCAT GGAACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pik3c2b ( XM_006529531.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529594.1 Mus musculus phosphoinositide-3-kinase, class 2, beta polypeptide (Pik3c2b), transcript variant X1, mRNA.

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CloneID	OMu02575
Clone ID Related Accession (Same CDS sequence)	XM_006529531.3 , XM_006529532.2 , NM_001099276.2 , XM_006529531.4
Accession Version	XM_006529531.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4899bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006529531.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTCCA CTCAGGGCAA TGGGGAGCAC TGGAAGTCCC TGGAGTCGGT GGGCATCAGC 
CGCAAAGAGC TGGCTATGGC CGAAGCCCTG CAGATGGAGT ATGACGCTCT TTCCCGGCTC 
CGACATCACA AGGAGGAGAG CAGGGCCAAG CAGAACACAG AACCCTCTCT TATCAGCTGG 
GATGAGCCAG CCTTGGACTT CTACAGCAAG CCAGCAGGCA GGCGGACCGA CCTCAAGCTC 
TTGCGTGGTC TTTCTGGCTC TGATCCCACC CTAAATTACA ACTCCATCTC CCCACCAGAG 
GGGCTCCCCA ACTCTACCTC CCAGGATCCA CAGCCTGGCC CAGACCCCTG GCCCAAGGGC 
TCCCTGTCTG GAGACTATCT CTATATTTTT GATGGCTCAG AGGGGAGATG CTCTTTGTCT 
CCAGGATCAG GTGACACAGA TGGCTCTTGC AAGAAACTAT CCCCACCTCC TCTCCCTCCT 
CGTGTCTCTA TCTGGGATGC CCCTCCCTTG CCTCCTAGAA AAGGATCACC CTCACCCTCC 
AAGATCTCTC AGCCTAATGA CATCAACAGT TTCTCTTCGG CAGAACAGCC ACCTGACAAA 
CTGCTAGTGG CCCAGGACCC AGAAGAGGGA GAGCTGCCTG ATGGCAGGGG ACAGGGACAC 
ACGCTGGGGT CTGTGGACTA CGATGGCATC AATGATGCAA TTACCAGGCT CAACTTGAAG 
TCCACCTACG ACTCAGAGAT ATCGTCTGAT GCTACCAGGG GCTGGAAGGA GGGCCGAGGG 
CCCCTGGACT TCAACAAGGA CACCTCTGGG AAACCTGTGG CCCGGAGCAA GACGATGCCC 
CCTCAGGTGC CCCCTCGAAC CTATACTTCT CGATATGCCA ACCGAAAAAA TGCAACACCT 
GGCAATAACC GACGGATTTC TGCTGCTCCG GTGGGCTCTC GGCCCCACAC TGTTGCCAAT 
GGCCACGAAT TGTTTGAGGT CTCAGAGGAG AGAGATGAGG AAGTTGCTGC GTTCTGCCAC 
ATGCTGGATA TCCTTCGAAC GGGCTCTGAT ATCCAGGACT ATTCCCTAAC TGGGTGTGTC 
TGGAGCACTG TCACCCCAAG TCCAGAACAC CTTGGGGATG AGGTCAACCT GAAGGTGACA 
GTGTTGTGTG ACAGTCTCCG GGAGCCACTC ACCTTTACCT GCAACTGTTC CTCCACCGTG 
GACCTGCTCA TCCACCAGAC CCTCTGCTAC ACTCACGATG AGCTGAGGGA AGTGGACGTG 
GGCGACTTTG TGCTGAAGCC CTGTGGGCTG GAGGAGTTCT TGCAAAATAA GCACGCCCTG 
GGAAGCCATG AGTATATCCA ATATTGCCGC AAGTTTGATA TCAGCATCCG GCTACAGCTG 
ATGGAGCAGA AGGCTATCCG GAGCGACCTG GCCCGGACGG TGAATGACGA CCAGAGCCCT 
TCCACCTTGA ACTACCTCAT CCATCTCCAA GAGAGGCCAG TCAAGCAGAC CATCAGCAGG 
CAGGCCTTGA GTCTTCTGTT TGACACTTAC CACAATGAGG TGGACGCCTT CCTGCTGGCT 
GATGGGGATT TCCCACTGAA GGCTGACAGG GTGGTCCAGT CTGTCAAGGC CATTTGCAAT 
GCTCTGGCTG CTGTGGAGAC TCCCGAGATC ACGAGCGCTC TCAACCAGCT GCCTCCCTGC 
CCCTCGCGCA TGCAGCCAAA AATCCAGAAG GATCCCAGCG TGTTGAGTGT GAGGGAAAAC 
CGAGAGAAGG TGGTAGAAGC CTTGACAGCG GCCATCTTGG ACTTGGTGGA GCTGTACTGC 
AGCACATTCA ATGCAGACTT CCAGACGGCG GTGCCTGGGA GCCGAAAGCA TGATTTGGTT 
CAAGAGGCCT GCCACTTCCC TGGGGCCCTG GCCTTCACTG TCTATGGCAC TCACCGTATT 
CCCATCATCT GGGCTACCAG CTATGAAGAT TTCTACCTCT CCTGCTCCCT CAGCCACGGT 
GGCAAGGAAC TTTGCAGCCC CCTGCAGACC CGGCGGGCTC ACTTCTCCAA GTACCTGTTC 
CACCTCATCA TCTGGGACCA GCAGATCTGC TTTCCAGTGC AGGTGAACAG GCTACCTCGG 
GAGACTCTGC TGTGTGCCAC ACTCTATGCC CTGCCCGTTC CCCCTCCTGG GGGTTCCTCT 
GAAGCGAATA AGCAGAAGCG GGTGCCTGAA GCTCTGGGTT GGGTCACTAC CCCTCTCTTC 
AACTTCAGGC AAGTCTTGAC CTGTGGTCGA AAGCTCCTGG GCTTGTGGCC AGCGACACAG 
GAAAACTCCG GTGCCCGCTG GAGTGCGCCT AATTTCCACC AGCCAGACAG TGTTATCCTG 
CAGATTGACT TCCCCAGCTC GGCCTTTGAC ATCAAGTTCA CCAGCCCCCC TGGAGACAAG 
TTCAGCCCCC GCTATGAGTT TGGCAGTCTC CGGGAGGAGG ACCAGCGCAA ACTTAAAGAC 
ATTACGCAGA AGGAGTCCCT ATACTGGCTC ACGGATGCTG ACAAGAAGCA GCTGTGGGAG 
AAACGCTATT ACTGCCACAC TGAGGTGAGC TCACTCCCCC TGGTTCTCGC CAGTGCACCC 
AGCTGGGAGT GGGCTTGCCT GCCGGACATC TATGCTCTCC TGCAACAGTG GACTCACATG 
AACCACCAAG ATGCCCTGGG ACTCCTGCAT GCCACCTTCC CGGACCAGGA GGTGCGGCGG 
ATGGCCGTGC AGTGGATCGG CTCCCTCTCA GATGCGGAGC TGCTGGACTA CCTGCCCCAG 
CTAGTCCAGG CCCTGAAGTA TGAGTGCTAC CTGGATAGCC CCTTGGTGCG CTTTCTCCTG 
AAACGAGCCA TCTCTGACCT GCGAGTGACC CACTACTTCT TCTGGTTACT GAAGGACAGC 
CTCAAGGACT CTCAGTTCAG TATCCGCTAC CAATACCTGC TAGCAGCCCT GCTGTGCTGC 
TGTGGCAAGG GACTGAGGGA GGAGTTTAAC CGCCAGTGCT GGCTTGTCAA CACCCTGGCC 
AAACTGGCCC AGCAGGTCCG AGAGGCCACC CCATCCGCCC GCCAGGGCAT CCTTCGTGTG 
GGCTTGGAGG AGGTGAAGCA GTTCTTTGCC CTCAATGGTT CGTGCCGTCT GCCGCTCAGT 
CCCAGTCTCC TGGTGAAGGG CATCGTGCCC AGGGACTGCT CCTACTTCAA TTCCAACGCA 
GTGCCCCTGA AGCTTGCCTT CCAAAATGTG GATCCGCTGG GCGAGAACAT CAGAGTCATC 
TTTAAGTGTG GGGATGATCT TCGTCAGGAC ATGCTCACCC TGCAGATGAT TCGCATCATG 
AGCAAGATCT GGGTACAGGA GGGGCTGGAC ATGCGCATGG TCATCTTCCG CTGCTTCTCC 
ACTGGCCGAG GAAAAGGGAT GGTAGAGATG ATACCCAATG CAGAGACACT GCGCAAGATC 
CAGGTGGAAC ATGGGGTGAC TGGCTCCTTC AAGGACCGTC CCCTGGCCGA CTGGCTACAA 
AAACACAACC CTGGGGAGGA TGAATATGAA AAGGCTGTGG AGAACTTCAT CTACTCTTGT 
GCTGGCTGCT GTGTAGCTAC ATACATCTTG GGCATCTGTG ACAGACACAA TGACAACATC 
ATGCTAAAGA CCACGGGCCA CATGTTCCAT ATAGATTTCG GCCGCTTCCT GGGTCACGCA 
CAGATGTTTG GTAACATCAA GAGGGACCGT GCCCCGTTTG TCTTCACCTC AGACATGGCG 
TATGTCATCA ATGGGGGTGA CAAGCCTTCC AGCCGCTTCC ATGATTTTGT TGACCTTTGC 
TGCCAAGCCT ACAACCTCAT TCGCAAGCAC ACCCACCTCT TCCTCAACCT TCTGGGCCTG 
ATGCTGTCCT GTGGAATCCC CGAGCTCTCA GACCTGGAGG ACCTCAAGTA TGTGTATGAT 
GCCCTGAGAC CTCAGGACAC AGAGGCCAAT GCCACCACCT ACTTCACAAG GTTGATTGAG 
TCCAGCCTGG GCAGCGTAGC CACGAAACTC AACTTTTTCA TCCATAACCT GGCTCAGATG 
AAGTTCACAG GCTCAGATGA CCGGCTGACC CTCTCCTTCG CTCCCCGGAC ACACACTCTC 
AAGAGCTCTG GCCGCATCCG AGATGTCTTC CTCTGCCGAC ATGAGAAGAT CTTCCACCCC 
AGCAAGGGCT ATGTATATGT GGTGAAGGTG ATGCGAGAGA ATGCTCACGA GGCGACATAC 
ATCCAGAGGA CATTCGAGGA GTTCCAGGAA CTGCACAATA AGCTGCGCCT GCTCTTCCCT 
TCTTCCTTCC TGCCCAGCTT CCCTAGCCGG TTTGTGATTG GCCGCTCCCG GGGAGAGGCA 
GTGGCTGAGA GGAGGAAGGA GGAGTTAAAT GGCTACATCT GGCACCTGAT TCATGCAGCC 
CCTGAGGTGG CTGAGTGTGA TCTGGTGTAT ACTTTCTTCC ACCCCCTGCC CCGAGATGAG 
AAGGCTTCAG GCCCCAGCCC AGCTCCGAAG TCCTCAGATG GGACGTGGGC CCGGCCGGTT 
GGGAAGGTCG GTGGGGAGGT GAAGCTGTCT ATCTCCTACA AAAACAACAA ACTCTTCATC 
ATGGTGATGC ATATTCGGGG CTTGCAACCG CTCCAGGATG GCAATGACCC TGACCCCTAT 
GTAAAGATTT ACCTCCTTCC TGACCCTCAG AAAACCACAA AGAGGAAAAC CAAGGTGGCC 
CGGAAAACCT GCAACCCTAC CTACAATGAG ATGCTGGTAT ATGATGGGAT CCCCAAGGGA 
GACCTGCAGC AGCGGGAGCT ACAGTTGAGT GTGCTGAGTG AGCAAGGATT TTGGGAGAAT 
CTGCTTCTCG GGGAGGTGCA CATCCGCCTT CGGGAGCTGG ACCTGGCCCA GGAGAAGACC 
GGCTGGTTTG GACTGGGTTC TCGAGGTCAT GGAACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529594.1
CDS	311..5209
Translation	MSSTQGNGEHWKSLESVGISRKELAMAEALQMEYDALSRLRHHKEESRAKQNTEPSLISWDEPALDFYSKPAGRRTDLKLLRGLSGSDPTLNYNSISPPEGLPNSTSQDPQPGPDPWPKGSLSGDYLYIFDGSEGRCSLSPGSGDTDGSCKKLSPPPLPPRVSIWDAPPLPPRKGSPSPSKISQPNDINSFSSAEQPPDKLLVAQDPEEGELPDGRGQGHTLGSVDYDGINDAITRLNLKSTYDSEISSDATRGWKEGRGPLDFNKDTSGKPVARSKTMPPQVPPRTYTSRYANRKNATPGNNRRISAAPVGSRPHTVANGHELFEVSEERDEEVAAFCHMLDILRTGSDIQDYSLTGCVWSTVTPSPEHLGDEVNLKVTVLCDSLREPLTFTCNCSSTVDLLIHQTLCYTHDELREVDVGDFVLKPCGLEEFLQNKHALGSHEYIQYCRKFDISIRLQLMEQKAIRSDLARTVNDDQSPSTLNYLIHLQERPVKQTISRQALSLLFDTYHNEVDAFLLADGDFPLKADRVVQSVKAICNALAAVETPEITSALNQLPPCPSRMQPKIQKDPSVLSVRENREKVVEALTAAILDLVELYCSTFNADFQTAVPGSRKHDLVQEACHFPGALAFTVYGTHRIPIIWATSYEDFYLSCSLSHGGKELCSPLQTRRAHFSKYLFHLIIWDQQICFPVQVNRLPRETLLCATLYALPVPPPGGSSEANKQKRVPEALGWVTTPLFNFRQVLTCGRKLLGLWPATQENSGARWSAPNFHQPDSVILQIDFPSSAFDIKFTSPPGDKFSPRYEFGSLREEDQRKLKDITQKESLYWLTDADKKQLWEKRYYCHTEVSSLPLVLASAPSWEWACLPDIYALLQQWTHMNHQDALGLLHATFPDQEVRRMAVQWIGSLSDAELLDYLPQLVQALKYECYLDSPLVRFLLKRAISDLRVTHYFFWLLKDSLKDSQFSIRYQYLLAALLCCCGKGLREEFNRQCWLVNTLAKLAQQVREATPSARQGILRVGLEEVKQFFALNGSCRLPLSPSLLVKGIVPRDCSYFNSNAVPLKLAFQNVDPLGENIRVIFKCGDDLRQDMLTLQMIRIMSKIWVQEGLDMRMVIFRCFSTGRGKGMVEMIPNAETLRKIQVEHGVTGSFKDRPLADWLQKHNPGEDEYEKAVENFIYSCAGCCVATYILGICDRHNDNIMLKTTGHMFHIDFGRFLGHAQMFGNIKRDRAPFVFTSDMAYVINGGDKPSSRFHDFVDLCCQAYNLIRKHTHLFLNLLGLMLSCGIPELSDLEDLKYVYDALRPQDTEANATTYFTRLIESSLGSVATKLNFFIHNLAQMKFTGSDDRLTLSFAPRTHTLKSSGRIRDVFLCRHEKIFHPSKGYVYVVKVMRENAHEATYIQRTFEEFQELHNKLRLLFPSSFLPSFPSRFVIGRSRGEAVAERRKEELNGYIWHLIHAAPEVAECDLVYTFFHPLPRDEKASGPSPAPKSSDGTWARPVGKVGGEVKLSISYKNNKLFIMVMHIRGLQPLQDGNDPDPYVKIYLLPDPQKTTKRKTKVARKTCNPTYNEMLVYDGIPKGDLQQRELQLSVLSEQGFWENLLLGEVHIRLRELDLAQEKTGWFGLGSRGHGT

Target ORF information:

RefSeq Version	XM_006529531.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphoinositide-3-kinase, class 2, beta polypeptide (Pik3c2b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529531.3

ORF Insert Sequence:

ATGTCTTCCA CTCAGGGCAA TGGGGAGCAC TGGAAGTCCC TGGAGTCGGT GGGCATCAGC 
CGCAAAGAGC TGGCTATGGC CGAAGCCCTG CAGATGGAGT ATGACGCTCT TTCCCGGCTC 
CGACATCACA AGGAGGAGAG CAGGGCCAAG CAGAACACAG AACCCTCTCT TATCAGCTGG 
GATGAGCCAG CCTTGGACTT CTACAGCAAG CCAGCAGGCA GGCGGACCGA CCTCAAGCTC 
TTGCGTGGTC TTTCTGGCTC TGATCCCACC CTAAATTACA ACTCCATCTC CCCACCAGAG 
GGGCTCCCCA ACTCTACCTC CCAGGATCCA CAGCCTGGCC CAGACCCCTG GCCCAAGGGC 
TCCCTGTCTG GAGACTATCT CTATATTTTT GATGGCTCAG AGGGGAGATG CTCTTTGTCT 
CCAGGATCAG GTGACACAGA TGGCTCTTGC AAGAAACTAT CCCCACCTCC TCTCCCTCCT 
CGTGTCTCTA TCTGGGATGC CCCTCCCTTG CCTCCTAGAA AAGGATCACC CTCACCCTCC 
AAGATCTCTC AGCCTAATGA CATCAACAGT TTCTCTTCGG CAGAACAGCC ACCTGACAAA 
CTGCTAGTGG CCCAGGACCC AGAAGAGGGA GAGCTGCCTG ATGGCAGGGG ACAGGGACAC 
ACGCTGGGGT CTGTGGACTA CGATGGCATC AATGATGCAA TTACCAGGCT CAACTTGAAG 
TCCACCTACG ACTCAGAGAT ATCGTCTGAT GCTACCAGGG GCTGGAAGGA GGGCCGAGGG 
CCCCTGGACT TCAACAAGGA CACCTCTGGG AAACCTGTGG CCCGGAGCAA GACGATGCCC 
CCTCAGGTGC CCCCTCGAAC CTATACTTCT CGATATGCCA ACCGAAAAAA TGCAACACCT 
GGCAATAACC GACGGATTTC TGCTGCTCCG GTGGGCTCTC GGCCCCACAC TGTTGCCAAT 
GGCCACGAAT TGTTTGAGGT CTCAGAGGAG AGAGATGAGG AAGTTGCTGC GTTCTGCCAC 
ATGCTGGATA TCCTTCGAAC GGGCTCTGAT ATCCAGGACT ATTCCCTAAC TGGGTGTGTC 
TGGAGCACTG TCACCCCAAG TCCAGAACAC CTTGGGGATG AGGTCAACCT GAAGGTGACA 
GTGTTGTGTG ACAGTCTCCG GGAGCCACTC ACCTTTACCT GCAACTGTTC CTCCACCGTG 
GACCTGCTCA TCCACCAGAC CCTCTGCTAC ACTCACGATG AGCTGAGGGA AGTGGACGTG 
GGCGACTTTG TGCTGAAGCC CTGTGGGCTG GAGGAGTTCT TGCAAAATAA GCACGCCCTG 
GGAAGCCATG AGTATATCCA ATATTGCCGC AAGTTTGATA TCAGCATCCG GCTACAGCTG 
ATGGAGCAGA AGGCTATCCG GAGCGACCTG GCCCGGACGG TGAATGACGA CCAGAGCCCT 
TCCACCTTGA ACTACCTCAT CCATCTCCAA GAGAGGCCAG TCAAGCAGAC CATCAGCAGG 
CAGGCCTTGA GTCTTCTGTT TGACACTTAC CACAATGAGG TGGACGCCTT CCTGCTGGCT 
GATGGGGATT TCCCACTGAA GGCTGACAGG GTGGTCCAGT CTGTCAAGGC CATTTGCAAT 
GCTCTGGCTG CTGTGGAGAC TCCCGAGATC ACGAGCGCTC TCAACCAGCT GCCTCCCTGC 
CCCTCGCGCA TGCAGCCAAA AATCCAGAAG GATCCCAGCG TGTTGAGTGT GAGGGAAAAC 
CGAGAGAAGG TGGTAGAAGC CTTGACAGCG GCCATCTTGG ACTTGGTGGA GCTGTACTGC 
AGCACATTCA ATGCAGACTT CCAGACGGCG GTGCCTGGGA GCCGAAAGCA TGATTTGGTT 
CAAGAGGCCT GCCACTTCCC TGGGGCCCTG GCCTTCACTG TCTATGGCAC TCACCGTATT 
CCCATCATCT GGGCTACCAG CTATGAAGAT TTCTACCTCT CCTGCTCCCT CAGCCACGGT 
GGCAAGGAAC TTTGCAGCCC CCTGCAGACC CGGCGGGCTC ACTTCTCCAA GTACCTGTTC 
CACCTCATCA TCTGGGACCA GCAGATCTGC TTTCCAGTGC AGGTGAACAG GCTACCTCGG 
GAGACTCTGC TGTGTGCCAC ACTCTATGCC CTGCCCGTTC CCCCTCCTGG GGGTTCCTCT 
GAAGCGAATA AGCAGAAGCG GGTGCCTGAA GCTCTGGGTT GGGTCACTAC CCCTCTCTTC 
AACTTCAGGC AAGTCTTGAC CTGTGGTCGA AAGCTCCTGG GCTTGTGGCC AGCGACACAG 
GAAAACTCCG GTGCCCGCTG GAGTGCGCCT AATTTCCACC AGCCAGACAG TGTTATCCTG 
CAGATTGACT TCCCCAGCTC GGCCTTTGAC ATCAAGTTCA CCAGCCCCCC TGGAGACAAG 
TTCAGCCCCC GCTATGAGTT TGGCAGTCTC CGGGAGGAGG ACCAGCGCAA ACTTAAAGAC 
ATTACGCAGA AGGAGTCCCT ATACTGGCTC ACGGATGCTG ACAAGAAGCA GCTGTGGGAG 
AAACGCTATT ACTGCCACAC TGAGGTGAGC TCACTCCCCC TGGTTCTCGC CAGTGCACCC 
AGCTGGGAGT GGGCTTGCCT GCCGGACATC TATGCTCTCC TGCAACAGTG GACTCACATG 
AACCACCAAG ATGCCCTGGG ACTCCTGCAT GCCACCTTCC CGGACCAGGA GGTGCGGCGG 
ATGGCCGTGC AGTGGATCGG CTCCCTCTCA GATGCGGAGC TGCTGGACTA CCTGCCCCAG 
CTAGTCCAGG CCCTGAAGTA TGAGTGCTAC CTGGATAGCC CCTTGGTGCG CTTTCTCCTG 
AAACGAGCCA TCTCTGACCT GCGAGTGACC CACTACTTCT TCTGGTTACT GAAGGACAGC 
CTCAAGGACT CTCAGTTCAG TATCCGCTAC CAATACCTGC TAGCAGCCCT GCTGTGCTGC 
TGTGGCAAGG GACTGAGGGA GGAGTTTAAC CGCCAGTGCT GGCTTGTCAA CACCCTGGCC 
AAACTGGCCC AGCAGGTCCG AGAGGCCACC CCATCCGCCC GCCAGGGCAT CCTTCGTGTG 
GGCTTGGAGG AGGTGAAGCA GTTCTTTGCC CTCAATGGTT CGTGCCGTCT GCCGCTCAGT 
CCCAGTCTCC TGGTGAAGGG CATCGTGCCC AGGGACTGCT CCTACTTCAA TTCCAACGCA 
GTGCCCCTGA AGCTTGCCTT CCAAAATGTG GATCCGCTGG GCGAGAACAT CAGAGTCATC 
TTTAAGTGTG GGGATGATCT TCGTCAGGAC ATGCTCACCC TGCAGATGAT TCGCATCATG 
AGCAAGATCT GGGTACAGGA GGGGCTGGAC ATGCGCATGG TCATCTTCCG CTGCTTCTCC 
ACTGGCCGAG GAAAAGGGAT GGTAGAGATG ATACCCAATG CAGAGACACT GCGCAAGATC 
CAGGTGGAAC ATGGGGTGAC TGGCTCCTTC AAGGACCGTC CCCTGGCCGA CTGGCTACAA 
AAACACAACC CTGGGGAGGA TGAATATGAA AAGGCTGTGG AGAACTTCAT CTACTCTTGT 
GCTGGCTGCT GTGTAGCTAC ATACATCTTG GGCATCTGTG ACAGACACAA TGACAACATC 
ATGCTAAAGA CCACGGGCCA CATGTTCCAT ATAGATTTCG GCCGCTTCCT GGGTCACGCA 
CAGATGTTTG GTAACATCAA GAGGGACCGT GCCCCGTTTG TCTTCACCTC AGACATGGCG 
TATGTCATCA ATGGGGGTGA CAAGCCTTCC AGCCGCTTCC ATGATTTTGT TGACCTTTGC 
TGCCAAGCCT ACAACCTCAT TCGCAAGCAC ACCCACCTCT TCCTCAACCT TCTGGGCCTG 
ATGCTGTCCT GTGGAATCCC CGAGCTCTCA GACCTGGAGG ACCTCAAGTA TGTGTATGAT 
GCCCTGAGAC CTCAGGACAC AGAGGCCAAT GCCACCACCT ACTTCACAAG GTTGATTGAG 
TCCAGCCTGG GCAGCGTAGC CACGAAACTC AACTTTTTCA TCCATAACCT GGCTCAGATG 
AAGTTCACAG GCTCAGATGA CCGGCTGACC CTCTCCTTCG CTCCCCGGAC ACACACTCTC 
AAGAGCTCTG GCCGCATCCG AGATGTCTTC CTCTGCCGAC ATGAGAAGAT CTTCCACCCC 
AGCAAGGGCT ATGTATATGT GGTGAAGGTG ATGCGAGAGA ATGCTCACGA GGCGACATAC 
ATCCAGAGGA CATTCGAGGA GTTCCAGGAA CTGCACAATA AGCTGCGCCT GCTCTTCCCT 
TCTTCCTTCC TGCCCAGCTT CCCTAGCCGG TTTGTGATTG GCCGCTCCCG GGGAGAGGCA 
GTGGCTGAGA GGAGGAAGGA GGAGTTAAAT GGCTACATCT GGCACCTGAT TCATGCAGCC 
CCTGAGGTGG CTGAGTGTGA TCTGGTGTAT ACTTTCTTCC ACCCCCTGCC CCGAGATGAG 
AAGGCTTCAG GCCCCAGCCC AGCTCCGAAG TCCTCAGATG GGACGTGGGC CCGGCCGGTT 
GGGAAGGTCG GTGGGGAGGT GAAGCTGTCT ATCTCCTACA AAAACAACAA ACTCTTCATC 
ATGGTGATGC ATATTCGGGG CTTGCAACCG CTCCAGGATG GCAATGACCC TGACCCCTAT 
GTAAAGATTT ACCTCCTTCC TGACCCTCAG AAAACCACAA AGAGGAAAAC CAAGGTGGCC 
CGGAAAACCT GCAACCCTAC CTACAATGAG ATGCTGGTAT ATGATGGGAT CCCCAAGGGA 
GACCTGCAGC AGCGGGAGCT ACAGTTGAGT GTGCTGAGTG AGCAAGGATT TTGGGAGAAT 
CTGCTTCTCG GGGAGGTGCA CATCCGCCTT CGGGAGCTGG ACCTGGCCCA GGAGAAGACC 
GGCTGGTTTG GACTGGGTTC TCGAGGTCAT GGAACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pik3c2b ( XM_006529532.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529595.1 Mus musculus phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 beta (Pik3c2b), transcript variant X1, mRNA.

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CloneID	OMu02575
Clone ID Related Accession (Same CDS sequence)	XM_006529531.3 , XM_006529532.2 , NM_001099276.2 , XM_006529531.4
Accession Version	XM_006529532.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4899bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006529532.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTCCA CTCAGGGCAA TGGGGAGCAC TGGAAGTCCC TGGAGTCGGT GGGCATCAGC 
CGCAAAGAGC TGGCTATGGC CGAAGCCCTG CAGATGGAGT ATGACGCTCT TTCCCGGCTC 
CGACATCACA AGGAGGAGAG CAGGGCCAAG CAGAACACAG AACCCTCTCT TATCAGCTGG 
GATGAGCCAG CCTTGGACTT CTACAGCAAG CCAGCAGGCA GGCGGACCGA CCTCAAGCTC 
TTGCGTGGTC TTTCTGGCTC TGATCCCACC CTAAATTACA ACTCCATCTC CCCACCAGAG 
GGGCTCCCCA ACTCTACCTC CCAGGATCCA CAGCCTGGCC CAGACCCCTG GCCCAAGGGC 
TCCCTGTCTG GAGACTATCT CTATATTTTT GATGGCTCAG AGGGGAGATG CTCTTTGTCT 
CCAGGATCAG GTGACACAGA TGGCTCTTGC AAGAAACTAT CCCCACCTCC TCTCCCTCCT 
CGTGTCTCTA TCTGGGATGC CCCTCCCTTG CCTCCTAGAA AAGGATCACC CTCACCCTCC 
AAGATCTCTC AGCCTAATGA CATCAACAGT TTCTCTTCGG CAGAACAGCC ACCTGACAAA 
CTGCTAGTGG CCCAGGACCC AGAAGAGGGA GAGCTGCCTG ATGGCAGGGG ACAGGGACAC 
ACGCTGGGGT CTGTGGACTA CGATGGCATC AATGATGCAA TTACCAGGCT CAACTTGAAG 
TCCACCTACG ACTCAGAGAT ATCGTCTGAT GCTACCAGGG GCTGGAAGGA GGGCCGAGGG 
CCCCTGGACT TCAACAAGGA CACCTCTGGG AAACCTGTGG CCCGGAGCAA GACGATGCCC 
CCTCAGGTGC CCCCTCGAAC CTATACTTCT CGATATGCCA ACCGAAAAAA TGCAACACCT 
GGCAATAACC GACGGATTTC TGCTGCTCCG GTGGGCTCTC GGCCCCACAC TGTTGCCAAT 
GGCCACGAAT TGTTTGAGGT CTCAGAGGAG AGAGATGAGG AAGTTGCTGC GTTCTGCCAC 
ATGCTGGATA TCCTTCGAAC GGGCTCTGAT ATCCAGGACT ATTCCCTAAC TGGGTGTGTC 
TGGAGCACTG TCACCCCAAG TCCAGAACAC CTTGGGGATG AGGTCAACCT GAAGGTGACA 
GTGTTGTGTG ACAGTCTCCG GGAGCCACTC ACCTTTACCT GCAACTGTTC CTCCACCGTG 
GACCTGCTCA TCCACCAGAC CCTCTGCTAC ACTCACGATG AGCTGAGGGA AGTGGACGTG 
GGCGACTTTG TGCTGAAGCC CTGTGGGCTG GAGGAGTTCT TGCAAAATAA GCACGCCCTG 
GGAAGCCATG AGTATATCCA ATATTGCCGC AAGTTTGATA TCAGCATCCG GCTACAGCTG 
ATGGAGCAGA AGGCTATCCG GAGCGACCTG GCCCGGACGG TGAATGACGA CCAGAGCCCT 
TCCACCTTGA ACTACCTCAT CCATCTCCAA GAGAGGCCAG TCAAGCAGAC CATCAGCAGG 
CAGGCCTTGA GTCTTCTGTT TGACACTTAC CACAATGAGG TGGACGCCTT CCTGCTGGCT 
GATGGGGATT TCCCACTGAA GGCTGACAGG GTGGTCCAGT CTGTCAAGGC CATTTGCAAT 
GCTCTGGCTG CTGTGGAGAC TCCCGAGATC ACGAGCGCTC TCAACCAGCT GCCTCCCTGC 
CCCTCGCGCA TGCAGCCAAA AATCCAGAAG GATCCCAGCG TGTTGAGTGT GAGGGAAAAC 
CGAGAGAAGG TGGTAGAAGC CTTGACAGCG GCCATCTTGG ACTTGGTGGA GCTGTACTGC 
AGCACATTCA ATGCAGACTT CCAGACGGCG GTGCCTGGGA GCCGAAAGCA TGATTTGGTT 
CAAGAGGCCT GCCACTTCCC TGGGGCCCTG GCCTTCACTG TCTATGGCAC TCACCGTATT 
CCCATCATCT GGGCTACCAG CTATGAAGAT TTCTACCTCT CCTGCTCCCT CAGCCACGGT 
GGCAAGGAAC TTTGCAGCCC CCTGCAGACC CGGCGGGCTC ACTTCTCCAA GTACCTGTTC 
CACCTCATCA TCTGGGACCA GCAGATCTGC TTTCCAGTGC AGGTGAACAG GCTACCTCGG 
GAGACTCTGC TGTGTGCCAC ACTCTATGCC CTGCCCGTTC CCCCTCCTGG GGGTTCCTCT 
GAAGCGAATA AGCAGAAGCG GGTGCCTGAA GCTCTGGGTT GGGTCACTAC CCCTCTCTTC 
AACTTCAGGC AAGTCTTGAC CTGTGGTCGA AAGCTCCTGG GCTTGTGGCC AGCGACACAG 
GAAAACTCCG GTGCCCGCTG GAGTGCGCCT AATTTCCACC AGCCAGACAG TGTTATCCTG 
CAGATTGACT TCCCCAGCTC GGCCTTTGAC ATCAAGTTCA CCAGCCCCCC TGGAGACAAG 
TTCAGCCCCC GCTATGAGTT TGGCAGTCTC CGGGAGGAGG ACCAGCGCAA ACTTAAAGAC 
ATTACGCAGA AGGAGTCCCT ATACTGGCTC ACGGATGCTG ACAAGAAGCA GCTGTGGGAG 
AAACGCTATT ACTGCCACAC TGAGGTGAGC TCACTCCCCC TGGTTCTCGC CAGTGCACCC 
AGCTGGGAGT GGGCTTGCCT GCCGGACATC TATGCTCTCC TGCAACAGTG GACTCACATG 
AACCACCAAG ATGCCCTGGG ACTCCTGCAT GCCACCTTCC CGGACCAGGA GGTGCGGCGG 
ATGGCCGTGC AGTGGATCGG CTCCCTCTCA GATGCGGAGC TGCTGGACTA CCTGCCCCAG 
CTAGTCCAGG CCCTGAAGTA TGAGTGCTAC CTGGATAGCC CCTTGGTGCG CTTTCTCCTG 
AAACGAGCCA TCTCTGACCT GCGAGTGACC CACTACTTCT TCTGGTTACT GAAGGACAGC 
CTCAAGGACT CTCAGTTCAG TATCCGCTAC CAATACCTGC TAGCAGCCCT GCTGTGCTGC 
TGTGGCAAGG GACTGAGGGA GGAGTTTAAC CGCCAGTGCT GGCTTGTCAA CACCCTGGCC 
AAACTGGCCC AGCAGGTCCG AGAGGCCACC CCATCCGCCC GCCAGGGCAT CCTTCGTGTG 
GGCTTGGAGG AGGTGAAGCA GTTCTTTGCC CTCAATGGTT CGTGCCGTCT GCCGCTCAGT 
CCCAGTCTCC TGGTGAAGGG CATCGTGCCC AGGGACTGCT CCTACTTCAA TTCCAACGCA 
GTGCCCCTGA AGCTTGCCTT CCAAAATGTG GATCCGCTGG GCGAGAACAT CAGAGTCATC 
TTTAAGTGTG GGGATGATCT TCGTCAGGAC ATGCTCACCC TGCAGATGAT TCGCATCATG 
AGCAAGATCT GGGTACAGGA GGGGCTGGAC ATGCGCATGG TCATCTTCCG CTGCTTCTCC 
ACTGGCCGAG GAAAAGGGAT GGTAGAGATG ATACCCAATG CAGAGACACT GCGCAAGATC 
CAGGTGGAAC ATGGGGTGAC TGGCTCCTTC AAGGACCGTC CCCTGGCCGA CTGGCTACAA 
AAACACAACC CTGGGGAGGA TGAATATGAA AAGGCTGTGG AGAACTTCAT CTACTCTTGT 
GCTGGCTGCT GTGTAGCTAC ATACATCTTG GGCATCTGTG ACAGACACAA TGACAACATC 
ATGCTAAAGA CCACGGGCCA CATGTTCCAT ATAGATTTCG GCCGCTTCCT GGGTCACGCA 
CAGATGTTTG GTAACATCAA GAGGGACCGT GCCCCGTTTG TCTTCACCTC AGACATGGCG 
TATGTCATCA ATGGGGGTGA CAAGCCTTCC AGCCGCTTCC ATGATTTTGT TGACCTTTGC 
TGCCAAGCCT ACAACCTCAT TCGCAAGCAC ACCCACCTCT TCCTCAACCT TCTGGGCCTG 
ATGCTGTCCT GTGGAATCCC CGAGCTCTCA GACCTGGAGG ACCTCAAGTA TGTGTATGAT 
GCCCTGAGAC CTCAGGACAC AGAGGCCAAT GCCACCACCT ACTTCACAAG GTTGATTGAG 
TCCAGCCTGG GCAGCGTAGC CACGAAACTC AACTTTTTCA TCCATAACCT GGCTCAGATG 
AAGTTCACAG GCTCAGATGA CCGGCTGACC CTCTCCTTCG CTCCCCGGAC ACACACTCTC 
AAGAGCTCTG GCCGCATCCG AGATGTCTTC CTCTGCCGAC ATGAGAAGAT CTTCCACCCC 
AGCAAGGGCT ATGTATATGT GGTGAAGGTG ATGCGAGAGA ATGCTCACGA GGCGACATAC 
ATCCAGAGGA CATTCGAGGA GTTCCAGGAA CTGCACAATA AGCTGCGCCT GCTCTTCCCT 
TCTTCCTTCC TGCCCAGCTT CCCTAGCCGG TTTGTGATTG GCCGCTCCCG GGGAGAGGCA 
GTGGCTGAGA GGAGGAAGGA GGAGTTAAAT GGCTACATCT GGCACCTGAT TCATGCAGCC 
CCTGAGGTGG CTGAGTGTGA TCTGGTGTAT ACTTTCTTCC ACCCCCTGCC CCGAGATGAG 
AAGGCTTCAG GCCCCAGCCC AGCTCCGAAG TCCTCAGATG GGACGTGGGC CCGGCCGGTT 
GGGAAGGTCG GTGGGGAGGT GAAGCTGTCT ATCTCCTACA AAAACAACAA ACTCTTCATC 
ATGGTGATGC ATATTCGGGG CTTGCAACCG CTCCAGGATG GCAATGACCC TGACCCCTAT 
GTAAAGATTT ACCTCCTTCC TGACCCTCAG AAAACCACAA AGAGGAAAAC CAAGGTGGCC 
CGGAAAACCT GCAACCCTAC CTACAATGAG ATGCTGGTAT ATGATGGGAT CCCCAAGGGA 
GACCTGCAGC AGCGGGAGCT ACAGTTGAGT GTGCTGAGTG AGCAAGGATT TTGGGAGAAT 
CTGCTTCTCG GGGAGGTGCA CATCCGCCTT CGGGAGCTGG ACCTGGCCCA GGAGAAGACC 
GGCTGGTTTG GACTGGGTTC TCGAGGTCAT GGAACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529595.1
CDS	335..5233
Translation	MSSTQGNGEHWKSLESVGISRKELAMAEALQMEYDALSRLRHHKEESRAKQNTEPSLISWDEPALDFYSKPAGRRTDLKLLRGLSGSDPTLNYNSISPPEGLPNSTSQDPQPGPDPWPKGSLSGDYLYIFDGSEGRCSLSPGSGDTDGSCKKLSPPPLPPRVSIWDAPPLPPRKGSPSPSKISQPNDINSFSSAEQPPDKLLVAQDPEEGELPDGRGQGHTLGSVDYDGINDAITRLNLKSTYDSEISSDATRGWKEGRGPLDFNKDTSGKPVARSKTMPPQVPPRTYTSRYANRKNATPGNNRRISAAPVGSRPHTVANGHELFEVSEERDEEVAAFCHMLDILRTGSDIQDYSLTGCVWSTVTPSPEHLGDEVNLKVTVLCDSLREPLTFTCNCSSTVDLLIHQTLCYTHDELREVDVGDFVLKPCGLEEFLQNKHALGSHEYIQYCRKFDISIRLQLMEQKAIRSDLARTVNDDQSPSTLNYLIHLQERPVKQTISRQALSLLFDTYHNEVDAFLLADGDFPLKADRVVQSVKAICNALAAVETPEITSALNQLPPCPSRMQPKIQKDPSVLSVRENREKVVEALTAAILDLVELYCSTFNADFQTAVPGSRKHDLVQEACHFPGALAFTVYGTHRIPIIWATSYEDFYLSCSLSHGGKELCSPLQTRRAHFSKYLFHLIIWDQQICFPVQVNRLPRETLLCATLYALPVPPPGGSSEANKQKRVPEALGWVTTPLFNFRQVLTCGRKLLGLWPATQENSGARWSAPNFHQPDSVILQIDFPSSAFDIKFTSPPGDKFSPRYEFGSLREEDQRKLKDITQKESLYWLTDADKKQLWEKRYYCHTEVSSLPLVLASAPSWEWACLPDIYALLQQWTHMNHQDALGLLHATFPDQEVRRMAVQWIGSLSDAELLDYLPQLVQALKYECYLDSPLVRFLLKRAISDLRVTHYFFWLLKDSLKDSQFSIRYQYLLAALLCCCGKGLREEFNRQCWLVNTLAKLAQQVREATPSARQGILRVGLEEVKQFFALNGSCRLPLSPSLLVKGIVPRDCSYFNSNAVPLKLAFQNVDPLGENIRVIFKCGDDLRQDMLTLQMIRIMSKIWVQEGLDMRMVIFRCFSTGRGKGMVEMIPNAETLRKIQVEHGVTGSFKDRPLADWLQKHNPGEDEYEKAVENFIYSCAGCCVATYILGICDRHNDNIMLKTTGHMFHIDFGRFLGHAQMFGNIKRDRAPFVFTSDMAYVINGGDKPSSRFHDFVDLCCQAYNLIRKHTHLFLNLLGLMLSCGIPELSDLEDLKYVYDALRPQDTEANATTYFTRLIESSLGSVATKLNFFIHNLAQMKFTGSDDRLTLSFAPRTHTLKSSGRIRDVFLCRHEKIFHPSKGYVYVVKVMRENAHEATYIQRTFEEFQELHNKLRLLFPSSFLPSFPSRFVIGRSRGEAVAERRKEELNGYIWHLIHAAPEVAECDLVYTFFHPLPRDEKASGPSPAPKSSDGTWARPVGKVGGEVKLSISYKNNKLFIMVMHIRGLQPLQDGNDPDPYVKIYLLPDPQKTTKRKTKVARKTCNPTYNEMLVYDGIPKGDLQQRELQLSVLSEQGFWENLLLGEVHIRLRELDLAQEKTGWFGLGSRGHGT

Target ORF information:

RefSeq Version	XM_006529532.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 beta (Pik3c2b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529532.2

ORF Insert Sequence:

ATGTCTTCCA CTCAGGGCAA TGGGGAGCAC TGGAAGTCCC TGGAGTCGGT GGGCATCAGC 
CGCAAAGAGC TGGCTATGGC CGAAGCCCTG CAGATGGAGT ATGACGCTCT TTCCCGGCTC 
CGACATCACA AGGAGGAGAG CAGGGCCAAG CAGAACACAG AACCCTCTCT TATCAGCTGG 
GATGAGCCAG CCTTGGACTT CTACAGCAAG CCAGCAGGCA GGCGGACCGA CCTCAAGCTC 
TTGCGTGGTC TTTCTGGCTC TGATCCCACC CTAAATTACA ACTCCATCTC CCCACCAGAG 
GGGCTCCCCA ACTCTACCTC CCAGGATCCA CAGCCTGGCC CAGACCCCTG GCCCAAGGGC 
TCCCTGTCTG GAGACTATCT CTATATTTTT GATGGCTCAG AGGGGAGATG CTCTTTGTCT 
CCAGGATCAG GTGACACAGA TGGCTCTTGC AAGAAACTAT CCCCACCTCC TCTCCCTCCT 
CGTGTCTCTA TCTGGGATGC CCCTCCCTTG CCTCCTAGAA AAGGATCACC CTCACCCTCC 
AAGATCTCTC AGCCTAATGA CATCAACAGT TTCTCTTCGG CAGAACAGCC ACCTGACAAA 
CTGCTAGTGG CCCAGGACCC AGAAGAGGGA GAGCTGCCTG ATGGCAGGGG ACAGGGACAC 
ACGCTGGGGT CTGTGGACTA CGATGGCATC AATGATGCAA TTACCAGGCT CAACTTGAAG 
TCCACCTACG ACTCAGAGAT ATCGTCTGAT GCTACCAGGG GCTGGAAGGA GGGCCGAGGG 
CCCCTGGACT TCAACAAGGA CACCTCTGGG AAACCTGTGG CCCGGAGCAA GACGATGCCC 
CCTCAGGTGC CCCCTCGAAC CTATACTTCT CGATATGCCA ACCGAAAAAA TGCAACACCT 
GGCAATAACC GACGGATTTC TGCTGCTCCG GTGGGCTCTC GGCCCCACAC TGTTGCCAAT 
GGCCACGAAT TGTTTGAGGT CTCAGAGGAG AGAGATGAGG AAGTTGCTGC GTTCTGCCAC 
ATGCTGGATA TCCTTCGAAC GGGCTCTGAT ATCCAGGACT ATTCCCTAAC TGGGTGTGTC 
TGGAGCACTG TCACCCCAAG TCCAGAACAC CTTGGGGATG AGGTCAACCT GAAGGTGACA 
GTGTTGTGTG ACAGTCTCCG GGAGCCACTC ACCTTTACCT GCAACTGTTC CTCCACCGTG 
GACCTGCTCA TCCACCAGAC CCTCTGCTAC ACTCACGATG AGCTGAGGGA AGTGGACGTG 
GGCGACTTTG TGCTGAAGCC CTGTGGGCTG GAGGAGTTCT TGCAAAATAA GCACGCCCTG 
GGAAGCCATG AGTATATCCA ATATTGCCGC AAGTTTGATA TCAGCATCCG GCTACAGCTG 
ATGGAGCAGA AGGCTATCCG GAGCGACCTG GCCCGGACGG TGAATGACGA CCAGAGCCCT 
TCCACCTTGA ACTACCTCAT CCATCTCCAA GAGAGGCCAG TCAAGCAGAC CATCAGCAGG 
CAGGCCTTGA GTCTTCTGTT TGACACTTAC CACAATGAGG TGGACGCCTT CCTGCTGGCT 
GATGGGGATT TCCCACTGAA GGCTGACAGG GTGGTCCAGT CTGTCAAGGC CATTTGCAAT 
GCTCTGGCTG CTGTGGAGAC TCCCGAGATC ACGAGCGCTC TCAACCAGCT GCCTCCCTGC 
CCCTCGCGCA TGCAGCCAAA AATCCAGAAG GATCCCAGCG TGTTGAGTGT GAGGGAAAAC 
CGAGAGAAGG TGGTAGAAGC CTTGACAGCG GCCATCTTGG ACTTGGTGGA GCTGTACTGC 
AGCACATTCA ATGCAGACTT CCAGACGGCG GTGCCTGGGA GCCGAAAGCA TGATTTGGTT 
CAAGAGGCCT GCCACTTCCC TGGGGCCCTG GCCTTCACTG TCTATGGCAC TCACCGTATT 
CCCATCATCT GGGCTACCAG CTATGAAGAT TTCTACCTCT CCTGCTCCCT CAGCCACGGT 
GGCAAGGAAC TTTGCAGCCC CCTGCAGACC CGGCGGGCTC ACTTCTCCAA GTACCTGTTC 
CACCTCATCA TCTGGGACCA GCAGATCTGC TTTCCAGTGC AGGTGAACAG GCTACCTCGG 
GAGACTCTGC TGTGTGCCAC ACTCTATGCC CTGCCCGTTC CCCCTCCTGG GGGTTCCTCT 
GAAGCGAATA AGCAGAAGCG GGTGCCTGAA GCTCTGGGTT GGGTCACTAC CCCTCTCTTC 
AACTTCAGGC AAGTCTTGAC CTGTGGTCGA AAGCTCCTGG GCTTGTGGCC AGCGACACAG 
GAAAACTCCG GTGCCCGCTG GAGTGCGCCT AATTTCCACC AGCCAGACAG TGTTATCCTG 
CAGATTGACT TCCCCAGCTC GGCCTTTGAC ATCAAGTTCA CCAGCCCCCC TGGAGACAAG 
TTCAGCCCCC GCTATGAGTT TGGCAGTCTC CGGGAGGAGG ACCAGCGCAA ACTTAAAGAC 
ATTACGCAGA AGGAGTCCCT ATACTGGCTC ACGGATGCTG ACAAGAAGCA GCTGTGGGAG 
AAACGCTATT ACTGCCACAC TGAGGTGAGC TCACTCCCCC TGGTTCTCGC CAGTGCACCC 
AGCTGGGAGT GGGCTTGCCT GCCGGACATC TATGCTCTCC TGCAACAGTG GACTCACATG 
AACCACCAAG ATGCCCTGGG ACTCCTGCAT GCCACCTTCC CGGACCAGGA GGTGCGGCGG 
ATGGCCGTGC AGTGGATCGG CTCCCTCTCA GATGCGGAGC TGCTGGACTA CCTGCCCCAG 
CTAGTCCAGG CCCTGAAGTA TGAGTGCTAC CTGGATAGCC CCTTGGTGCG CTTTCTCCTG 
AAACGAGCCA TCTCTGACCT GCGAGTGACC CACTACTTCT TCTGGTTACT GAAGGACAGC 
CTCAAGGACT CTCAGTTCAG TATCCGCTAC CAATACCTGC TAGCAGCCCT GCTGTGCTGC 
TGTGGCAAGG GACTGAGGGA GGAGTTTAAC CGCCAGTGCT GGCTTGTCAA CACCCTGGCC 
AAACTGGCCC AGCAGGTCCG AGAGGCCACC CCATCCGCCC GCCAGGGCAT CCTTCGTGTG 
GGCTTGGAGG AGGTGAAGCA GTTCTTTGCC CTCAATGGTT CGTGCCGTCT GCCGCTCAGT 
CCCAGTCTCC TGGTGAAGGG CATCGTGCCC AGGGACTGCT CCTACTTCAA TTCCAACGCA 
GTGCCCCTGA AGCTTGCCTT CCAAAATGTG GATCCGCTGG GCGAGAACAT CAGAGTCATC 
TTTAAGTGTG GGGATGATCT TCGTCAGGAC ATGCTCACCC TGCAGATGAT TCGCATCATG 
AGCAAGATCT GGGTACAGGA GGGGCTGGAC ATGCGCATGG TCATCTTCCG CTGCTTCTCC 
ACTGGCCGAG GAAAAGGGAT GGTAGAGATG ATACCCAATG CAGAGACACT GCGCAAGATC 
CAGGTGGAAC ATGGGGTGAC TGGCTCCTTC AAGGACCGTC CCCTGGCCGA CTGGCTACAA 
AAACACAACC CTGGGGAGGA TGAATATGAA AAGGCTGTGG AGAACTTCAT CTACTCTTGT 
GCTGGCTGCT GTGTAGCTAC ATACATCTTG GGCATCTGTG ACAGACACAA TGACAACATC 
ATGCTAAAGA CCACGGGCCA CATGTTCCAT ATAGATTTCG GCCGCTTCCT GGGTCACGCA 
CAGATGTTTG GTAACATCAA GAGGGACCGT GCCCCGTTTG TCTTCACCTC AGACATGGCG 
TATGTCATCA ATGGGGGTGA CAAGCCTTCC AGCCGCTTCC ATGATTTTGT TGACCTTTGC 
TGCCAAGCCT ACAACCTCAT TCGCAAGCAC ACCCACCTCT TCCTCAACCT TCTGGGCCTG 
ATGCTGTCCT GTGGAATCCC CGAGCTCTCA GACCTGGAGG ACCTCAAGTA TGTGTATGAT 
GCCCTGAGAC CTCAGGACAC AGAGGCCAAT GCCACCACCT ACTTCACAAG GTTGATTGAG 
TCCAGCCTGG GCAGCGTAGC CACGAAACTC AACTTTTTCA TCCATAACCT GGCTCAGATG 
AAGTTCACAG GCTCAGATGA CCGGCTGACC CTCTCCTTCG CTCCCCGGAC ACACACTCTC 
AAGAGCTCTG GCCGCATCCG AGATGTCTTC CTCTGCCGAC ATGAGAAGAT CTTCCACCCC 
AGCAAGGGCT ATGTATATGT GGTGAAGGTG ATGCGAGAGA ATGCTCACGA GGCGACATAC 
ATCCAGAGGA CATTCGAGGA GTTCCAGGAA CTGCACAATA AGCTGCGCCT GCTCTTCCCT 
TCTTCCTTCC TGCCCAGCTT CCCTAGCCGG TTTGTGATTG GCCGCTCCCG GGGAGAGGCA 
GTGGCTGAGA GGAGGAAGGA GGAGTTAAAT GGCTACATCT GGCACCTGAT TCATGCAGCC 
CCTGAGGTGG CTGAGTGTGA TCTGGTGTAT ACTTTCTTCC ACCCCCTGCC CCGAGATGAG 
AAGGCTTCAG GCCCCAGCCC AGCTCCGAAG TCCTCAGATG GGACGTGGGC CCGGCCGGTT 
GGGAAGGTCG GTGGGGAGGT GAAGCTGTCT ATCTCCTACA AAAACAACAA ACTCTTCATC 
ATGGTGATGC ATATTCGGGG CTTGCAACCG CTCCAGGATG GCAATGACCC TGACCCCTAT 
GTAAAGATTT ACCTCCTTCC TGACCCTCAG AAAACCACAA AGAGGAAAAC CAAGGTGGCC 
CGGAAAACCT GCAACCCTAC CTACAATGAG ATGCTGGTAT ATGATGGGAT CCCCAAGGGA 
GACCTGCAGC AGCGGGAGCT ACAGTTGAGT GTGCTGAGTG AGCAAGGATT TTGGGAGAAT 
CTGCTTCTCG GGGAGGTGCA CATCCGCCTT CGGGAGCTGG ACCTGGCCCA GGAGAAGACC 
GGCTGGTTTG GACTGGGTTC TCGAGGTCAT GGAACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pik3c2b ( XM_006529533.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529596.1 Mus musculus phosphoinositide-3-kinase, class 2, beta polypeptide (Pik3c2b), transcript variant X3, mRNA.

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CloneID	OMu28198
Clone ID Related Accession (Same CDS sequence)	XM_006529533.3 , XM_006529533.4
Accession Version	XM_006529533.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4236bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit beta isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006529533.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCAGT GGTCCCCCCC ACCTCAGCTC GGGGCAGGGG CACAGCCTGC TGGGAGGGGA 
GACCCCTTGA TCTCCGCTCC TATACCCCCA CCCCGCCTTC GCCGGGCTTC CGGCTGGAAG 
GATCCTACTT ATCCCAGGCC CACTGAGCCA TCTCTGGAAA GGGCTAGCTC CAAATGCCAG 
CCTGGTACCC CCGCTGCCAG CCTCTGCCTG GCAGACTCCC CCGACCAGCA TCGGACGATG 
CCACCAGGGC CTGCCAGCCG GGCAAACGTG GGCTCTCGGC CCCACACTGT TGCCAATGGC 
CACGAATTGT TTGAGGTCTC AGAGGAGAGA GATGAGGAAG TTGCTGCGTT CTGCCACATG 
CTGGATATCC TTCGAACGGG CTCTGATATC CAGGACTATT CCCTAACTGG GTGTGTCTGG 
AGCACTGTCA CCCCAAGTCC AGAACACCTT GGGGATGAGG TCAACCTGAA GGTGACAGTG 
TTGTGTGACA GTCTCCGGGA GCCACTCACC TTTACCTGCA ACTGTTCCTC CACCGTGGAC 
CTGCTCATCC ACCAGACCCT CTGCTACACT CACGATGAGC TGAGGGAAGT GGACGTGGGC 
GACTTTGTGC TGAAGCCCTG TGGGCTGGAG GAGTTCTTGC AAAATAAGCA CGCCCTGGGA 
AGCCATGAGT ATATCCAATA TTGCCGCAAG TTTGATATCA GCATCCGGCT ACAGCTGATG 
GAGCAGAAGG CTATCCGGAG CGACCTGGCC CGGACGGTGA ATGACGACCA GAGCCCTTCC 
ACCTTGAACT ACCTCATCCA TCTCCAAGAG AGGCCAGTCA AGCAGACCAT CAGCAGGCAG 
GCCTTGAGTC TTCTGTTTGA CACTTACCAC AATGAGGTGG ACGCCTTCCT GCTGGCTGAT 
GGGGATTTCC CACTGAAGGC TGACAGGGTG GTCCAGTCTG TCAAGGCCAT TTGCAATGCT 
CTGGCTGCTG TGGAGACTCC CGAGATCACG AGCGCTCTCA ACCAGCTGCC TCCCTGCCCC 
TCGCGCATGC AGCCAAAAAT CCAGAAGGAT CCCAGCGTGT TGAGTGTGAG GGAAAACCGA 
GAGAAGGTGG TAGAAGCCTT GACAGCGGCC ATCTTGGACT TGGTGGAGCT GTACTGCAGC 
ACATTCAATG CAGACTTCCA GACGGCGGTG CCTGGGAGCC GAAAGCATGA TTTGGTTCAA 
GAGGCCTGCC ACTTCCCTGG GGCCCTGGCC TTCACTGTCT ATGGCACTCA CCGTATTCCC 
ATCATCTGGG CTACCAGCTA TGAAGATTTC TACCTCTCCT GCTCCCTCAG CCACGGTGGC 
AAGGAACTTT GCAGCCCCCT GCAGACCCGG CGGGCTCACT TCTCCAAGTA CCTGTTCCAC 
CTCATCATCT GGGACCAGCA GATCTGCTTT CCAGTGCAGG TGAACAGGCT ACCTCGGGAG 
ACTCTGCTGT GTGCCACACT CTATGCCCTG CCCGTTCCCC CTCCTGGGGG TTCCTCTGAA 
GCGAATAAGC AGAAGCGGGT GCCTGAAGCT CTGGGTTGGG TCACTACCCC TCTCTTCAAC 
TTCAGGCAAG TCTTGACCTG TGGTCGAAAG CTCCTGGGCT TGTGGCCAGC GACACAGGAA 
AACTCCGGTG CCCGCTGGAG TGCGCCTAAT TTCCACCAGC CAGACAGTGT TATCCTGCAG 
ATTGACTTCC CCAGCTCGGC CTTTGACATC AAGTTCACCA GCCCCCCTGG AGACAAGTTC 
AGCCCCCGCT ATGAGTTTGG CAGTCTCCGG GAGGAGGACC AGCGCAAACT TAAAGACATT 
ACGCAGAAGG AGTCCCTATA CTGGCTCACG GATGCTGACA AGAAGCAGCT GTGGGAGAAA 
CGCTATTACT GCCACACTGA GGTGAGCTCA CTCCCCCTGG TTCTCGCCAG TGCACCCAGC 
TGGGAGTGGG CTTGCCTGCC GGACATCTAT GCTCTCCTGC AACAGTGGAC TCACATGAAC 
CACCAAGATG CCCTGGGACT CCTGCATGCC ACCTTCCCGG ACCAGGAGGT GCGGCGGATG 
GCCGTGCAGT GGATCGGCTC CCTCTCAGAT GCGGAGCTGC TGGACTACCT GCCCCAGCTA 
GTCCAGGCCC TGAAGTATGA GTGCTACCTG GATAGCCCCT TGGTGCGCTT TCTCCTGAAA 
CGAGCCATCT CTGACCTGCG AGTGACCCAC TACTTCTTCT GGTTACTGAA GGACAGCCTC 
AAGGACTCTC AGTTCAGTAT CCGCTACCAA TACCTGCTAG CAGCCCTGCT GTGCTGCTGT 
GGCAAGGGAC TGAGGGAGGA GTTTAACCGC CAGTGCTGGC TTGTCAACAC CCTGGCCAAA 
CTGGCCCAGC AGGTCCGAGA GGCCACCCCA TCCGCCCGCC AGGGCATCCT TCGTGTGGGC 
TTGGAGGAGG TGAAGCAGTT CTTTGCCCTC AATGGTTCGT GCCGTCTGCC GCTCAGTCCC 
AGTCTCCTGG TGAAGGGCAT CGTGCCCAGG GACTGCTCCT ACTTCAATTC CAACGCAGTG 
CCCCTGAAGC TTGCCTTCCA AAATGTGGAT CCGCTGGGCG AGAACATCAG AGTCATCTTT 
AAGTGTGGGG ATGATCTTCG TCAGGACATG CTCACCCTGC AGATGATTCG CATCATGAGC 
AAGATCTGGG TACAGGAGGG GCTGGACATG CGCATGGTCA TCTTCCGCTG CTTCTCCACT 
GGCCGAGGAA AAGGGATGGT AGAGATGATA CCCAATGCAG AGACACTGCG CAAGATCCAG 
GTGGAACATG GGGTGACTGG CTCCTTCAAG GACCGTCCCC TGGCCGACTG GCTACAAAAA 
CACAACCCTG GGGAGGATGA ATATGAAAAG GCTGTGGAGA ACTTCATCTA CTCTTGTGCT 
GGCTGCTGTG TAGCTACATA CATCTTGGGC ATCTGTGACA GACACAATGA CAACATCATG 
CTAAAGACCA CGGGCCACAT GTTCCATATA GATTTCGGCC GCTTCCTGGG TCACGCACAG 
ATGTTTGGTA ACATCAAGAG GGACCGTGCC CCGTTTGTCT TCACCTCAGA CATGGCGTAT 
GTCATCAATG GGGGTGACAA GCCTTCCAGC CGCTTCCATG ATTTTGTTGA CCTTTGCTGC 
CAAGCCTACA ACCTCATTCG CAAGCACACC CACCTCTTCC TCAACCTTCT GGGCCTGATG 
CTGTCCTGTG GAATCCCCGA GCTCTCAGAC CTGGAGGACC TCAAGTATGT GTATGATGCC 
CTGAGACCTC AGGACACAGA GGCCAATGCC ACCACCTACT TCACAAGGTT GATTGAGTCC 
AGCCTGGGCA GCGTAGCCAC GAAACTCAAC TTTTTCATCC ATAACCTGGC TCAGATGAAG 
TTCACAGGCT CAGATGACCG GCTGACCCTC TCCTTCGCTC CCCGGACACA CACTCTCAAG 
AGCTCTGGCC GCATCCGAGA TGTCTTCCTC TGCCGACATG AGAAGATCTT CCACCCCAGC 
AAGGGCTATG TATATGTGGT GAAGGTGATG CGAGAGAATG CTCACGAGGC GACATACATC 
CAGAGGACAT TCGAGGAGTT CCAGGAACTG CACAATAAGC TGCGCCTGCT CTTCCCTTCT 
TCCTTCCTGC CCAGCTTCCC TAGCCGGTTT GTGATTGGCC GCTCCCGGGG AGAGGCAGTG 
GCTGAGAGGA GGAAGGAGGA GTTAAATGGC TACATCTGGC ACCTGATTCA TGCAGCCCCT 
GAGGTGGCTG AGTGTGATCT GGTGTATACT TTCTTCCACC CCCTGCCCCG AGATGAGAAG 
GCTTCAGGCC CCAGCCCAGC TCCGAAGTCC TCAGATGGGA CGTGGGCCCG GCCGGTTGGG 
AAGGTCGGTG GGGAGGTGAA GCTGTCTATC TCCTACAAAA ACAACAAACT CTTCATCATG 
GTGATGCATA TTCGGGGCTT GCAACCGCTC CAGGATGGCA ATGACCCTGA CCCCTATGTA 
AAGATTTACC TCCTTCCTGA CCCTCAGAAA ACCACAAAGA GGAAAACCAA GGTGGCCCGG 
AAAACCTGCA ACCCTACCTA CAATGAGATG CTGGTATATG ATGGGATCCC CAAGGGAGAC 
CTGCAGCAGC GGGAGCTACA GTTGAGTGTG CTGAGTGAGC AAGGATTTTG GGAGAATCTG 
CTTCTCGGGG AGGTGCACAT CCGCCTTCGG GAGCTGGACC TGGCCCAGGA GAAGACCGGC 
TGGTTTGGAC TGGGTTCTCG AGGTCATGGA ACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529596.1
CDS	24..4259
Translation	MRQWSPPPQLGAGAQPAGRGDPLISAPIPPPRLRRASGWKDPTYPRPTEPSLERASSKCQPGTPAASLCLADSPDQHRTMPPGPASRANVGSRPHTVANGHELFEVSEERDEEVAAFCHMLDILRTGSDIQDYSLTGCVWSTVTPSPEHLGDEVNLKVTVLCDSLREPLTFTCNCSSTVDLLIHQTLCYTHDELREVDVGDFVLKPCGLEEFLQNKHALGSHEYIQYCRKFDISIRLQLMEQKAIRSDLARTVNDDQSPSTLNYLIHLQERPVKQTISRQALSLLFDTYHNEVDAFLLADGDFPLKADRVVQSVKAICNALAAVETPEITSALNQLPPCPSRMQPKIQKDPSVLSVRENREKVVEALTAAILDLVELYCSTFNADFQTAVPGSRKHDLVQEACHFPGALAFTVYGTHRIPIIWATSYEDFYLSCSLSHGGKELCSPLQTRRAHFSKYLFHLIIWDQQICFPVQVNRLPRETLLCATLYALPVPPPGGSSEANKQKRVPEALGWVTTPLFNFRQVLTCGRKLLGLWPATQENSGARWSAPNFHQPDSVILQIDFPSSAFDIKFTSPPGDKFSPRYEFGSLREEDQRKLKDITQKESLYWLTDADKKQLWEKRYYCHTEVSSLPLVLASAPSWEWACLPDIYALLQQWTHMNHQDALGLLHATFPDQEVRRMAVQWIGSLSDAELLDYLPQLVQALKYECYLDSPLVRFLLKRAISDLRVTHYFFWLLKDSLKDSQFSIRYQYLLAALLCCCGKGLREEFNRQCWLVNTLAKLAQQVREATPSARQGILRVGLEEVKQFFALNGSCRLPLSPSLLVKGIVPRDCSYFNSNAVPLKLAFQNVDPLGENIRVIFKCGDDLRQDMLTLQMIRIMSKIWVQEGLDMRMVIFRCFSTGRGKGMVEMIPNAETLRKIQVEHGVTGSFKDRPLADWLQKHNPGEDEYEKAVENFIYSCAGCCVATYILGICDRHNDNIMLKTTGHMFHIDFGRFLGHAQMFGNIKRDRAPFVFTSDMAYVINGGDKPSSRFHDFVDLCCQAYNLIRKHTHLFLNLLGLMLSCGIPELSDLEDLKYVYDALRPQDTEANATTYFTRLIESSLGSVATKLNFFIHNLAQMKFTGSDDRLTLSFAPRTHTLKSSGRIRDVFLCRHEKIFHPSKGYVYVVKVMRENAHEATYIQRTFEEFQELHNKLRLLFPSSFLPSFPSRFVIGRSRGEAVAERRKEELNGYIWHLIHAAPEVAECDLVYTFFHPLPRDEKASGPSPAPKSSDGTWARPVGKVGGEVKLSISYKNNKLFIMVMHIRGLQPLQDGNDPDPYVKIYLLPDPQKTTKRKTKVARKTCNPTYNEMLVYDGIPKGDLQQRELQLSVLSEQGFWENLLLGEVHIRLRELDLAQEKTGWFGLGSRGHGT

Target ORF information:

RefSeq Version	XM_006529533.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphoinositide-3-kinase, class 2, beta polypeptide (Pik3c2b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529533.3

ORF Insert Sequence:

ATGCGGCAGT GGTCCCCCCC ACCTCAGCTC GGGGCAGGGG CACAGCCTGC TGGGAGGGGA 
GACCCCTTGA TCTCCGCTCC TATACCCCCA CCCCGCCTTC GCCGGGCTTC CGGCTGGAAG 
GATCCTACTT ATCCCAGGCC CACTGAGCCA TCTCTGGAAA GGGCTAGCTC CAAATGCCAG 
CCTGGTACCC CCGCTGCCAG CCTCTGCCTG GCAGACTCCC CCGACCAGCA TCGGACGATG 
CCACCAGGGC CTGCCAGCCG GGCAAACGTG GGCTCTCGGC CCCACACTGT TGCCAATGGC 
CACGAATTGT TTGAGGTCTC AGAGGAGAGA GATGAGGAAG TTGCTGCGTT CTGCCACATG 
CTGGATATCC TTCGAACGGG CTCTGATATC CAGGACTATT CCCTAACTGG GTGTGTCTGG 
AGCACTGTCA CCCCAAGTCC AGAACACCTT GGGGATGAGG TCAACCTGAA GGTGACAGTG 
TTGTGTGACA GTCTCCGGGA GCCACTCACC TTTACCTGCA ACTGTTCCTC CACCGTGGAC 
CTGCTCATCC ACCAGACCCT CTGCTACACT CACGATGAGC TGAGGGAAGT GGACGTGGGC 
GACTTTGTGC TGAAGCCCTG TGGGCTGGAG GAGTTCTTGC AAAATAAGCA CGCCCTGGGA 
AGCCATGAGT ATATCCAATA TTGCCGCAAG TTTGATATCA GCATCCGGCT ACAGCTGATG 
GAGCAGAAGG CTATCCGGAG CGACCTGGCC CGGACGGTGA ATGACGACCA GAGCCCTTCC 
ACCTTGAACT ACCTCATCCA TCTCCAAGAG AGGCCAGTCA AGCAGACCAT CAGCAGGCAG 
GCCTTGAGTC TTCTGTTTGA CACTTACCAC AATGAGGTGG ACGCCTTCCT GCTGGCTGAT 
GGGGATTTCC CACTGAAGGC TGACAGGGTG GTCCAGTCTG TCAAGGCCAT TTGCAATGCT 
CTGGCTGCTG TGGAGACTCC CGAGATCACG AGCGCTCTCA ACCAGCTGCC TCCCTGCCCC 
TCGCGCATGC AGCCAAAAAT CCAGAAGGAT CCCAGCGTGT TGAGTGTGAG GGAAAACCGA 
GAGAAGGTGG TAGAAGCCTT GACAGCGGCC ATCTTGGACT TGGTGGAGCT GTACTGCAGC 
ACATTCAATG CAGACTTCCA GACGGCGGTG CCTGGGAGCC GAAAGCATGA TTTGGTTCAA 
GAGGCCTGCC ACTTCCCTGG GGCCCTGGCC TTCACTGTCT ATGGCACTCA CCGTATTCCC 
ATCATCTGGG CTACCAGCTA TGAAGATTTC TACCTCTCCT GCTCCCTCAG CCACGGTGGC 
AAGGAACTTT GCAGCCCCCT GCAGACCCGG CGGGCTCACT TCTCCAAGTA CCTGTTCCAC 
CTCATCATCT GGGACCAGCA GATCTGCTTT CCAGTGCAGG TGAACAGGCT ACCTCGGGAG 
ACTCTGCTGT GTGCCACACT CTATGCCCTG CCCGTTCCCC CTCCTGGGGG TTCCTCTGAA 
GCGAATAAGC AGAAGCGGGT GCCTGAAGCT CTGGGTTGGG TCACTACCCC TCTCTTCAAC 
TTCAGGCAAG TCTTGACCTG TGGTCGAAAG CTCCTGGGCT TGTGGCCAGC GACACAGGAA 
AACTCCGGTG CCCGCTGGAG TGCGCCTAAT TTCCACCAGC CAGACAGTGT TATCCTGCAG 
ATTGACTTCC CCAGCTCGGC CTTTGACATC AAGTTCACCA GCCCCCCTGG AGACAAGTTC 
AGCCCCCGCT ATGAGTTTGG CAGTCTCCGG GAGGAGGACC AGCGCAAACT TAAAGACATT 
ACGCAGAAGG AGTCCCTATA CTGGCTCACG GATGCTGACA AGAAGCAGCT GTGGGAGAAA 
CGCTATTACT GCCACACTGA GGTGAGCTCA CTCCCCCTGG TTCTCGCCAG TGCACCCAGC 
TGGGAGTGGG CTTGCCTGCC GGACATCTAT GCTCTCCTGC AACAGTGGAC TCACATGAAC 
CACCAAGATG CCCTGGGACT CCTGCATGCC ACCTTCCCGG ACCAGGAGGT GCGGCGGATG 
GCCGTGCAGT GGATCGGCTC CCTCTCAGAT GCGGAGCTGC TGGACTACCT GCCCCAGCTA 
GTCCAGGCCC TGAAGTATGA GTGCTACCTG GATAGCCCCT TGGTGCGCTT TCTCCTGAAA 
CGAGCCATCT CTGACCTGCG AGTGACCCAC TACTTCTTCT GGTTACTGAA GGACAGCCTC 
AAGGACTCTC AGTTCAGTAT CCGCTACCAA TACCTGCTAG CAGCCCTGCT GTGCTGCTGT 
GGCAAGGGAC TGAGGGAGGA GTTTAACCGC CAGTGCTGGC TTGTCAACAC CCTGGCCAAA 
CTGGCCCAGC AGGTCCGAGA GGCCACCCCA TCCGCCCGCC AGGGCATCCT TCGTGTGGGC 
TTGGAGGAGG TGAAGCAGTT CTTTGCCCTC AATGGTTCGT GCCGTCTGCC GCTCAGTCCC 
AGTCTCCTGG TGAAGGGCAT CGTGCCCAGG GACTGCTCCT ACTTCAATTC CAACGCAGTG 
CCCCTGAAGC TTGCCTTCCA AAATGTGGAT CCGCTGGGCG AGAACATCAG AGTCATCTTT 
AAGTGTGGGG ATGATCTTCG TCAGGACATG CTCACCCTGC AGATGATTCG CATCATGAGC 
AAGATCTGGG TACAGGAGGG GCTGGACATG CGCATGGTCA TCTTCCGCTG CTTCTCCACT 
GGCCGAGGAA AAGGGATGGT AGAGATGATA CCCAATGCAG AGACACTGCG CAAGATCCAG 
GTGGAACATG GGGTGACTGG CTCCTTCAAG GACCGTCCCC TGGCCGACTG GCTACAAAAA 
CACAACCCTG GGGAGGATGA ATATGAAAAG GCTGTGGAGA ACTTCATCTA CTCTTGTGCT 
GGCTGCTGTG TAGCTACATA CATCTTGGGC ATCTGTGACA GACACAATGA CAACATCATG 
CTAAAGACCA CGGGCCACAT GTTCCATATA GATTTCGGCC GCTTCCTGGG TCACGCACAG 
ATGTTTGGTA ACATCAAGAG GGACCGTGCC CCGTTTGTCT TCACCTCAGA CATGGCGTAT 
GTCATCAATG GGGGTGACAA GCCTTCCAGC CGCTTCCATG ATTTTGTTGA CCTTTGCTGC 
CAAGCCTACA ACCTCATTCG CAAGCACACC CACCTCTTCC TCAACCTTCT GGGCCTGATG 
CTGTCCTGTG GAATCCCCGA GCTCTCAGAC CTGGAGGACC TCAAGTATGT GTATGATGCC 
CTGAGACCTC AGGACACAGA GGCCAATGCC ACCACCTACT TCACAAGGTT GATTGAGTCC 
AGCCTGGGCA GCGTAGCCAC GAAACTCAAC TTTTTCATCC ATAACCTGGC TCAGATGAAG 
TTCACAGGCT CAGATGACCG GCTGACCCTC TCCTTCGCTC CCCGGACACA CACTCTCAAG 
AGCTCTGGCC GCATCCGAGA TGTCTTCCTC TGCCGACATG AGAAGATCTT CCACCCCAGC 
AAGGGCTATG TATATGTGGT GAAGGTGATG CGAGAGAATG CTCACGAGGC GACATACATC 
CAGAGGACAT TCGAGGAGTT CCAGGAACTG CACAATAAGC TGCGCCTGCT CTTCCCTTCT 
TCCTTCCTGC CCAGCTTCCC TAGCCGGTTT GTGATTGGCC GCTCCCGGGG AGAGGCAGTG 
GCTGAGAGGA GGAAGGAGGA GTTAAATGGC TACATCTGGC ACCTGATTCA TGCAGCCCCT 
GAGGTGGCTG AGTGTGATCT GGTGTATACT TTCTTCCACC CCCTGCCCCG AGATGAGAAG 
GCTTCAGGCC CCAGCCCAGC TCCGAAGTCC TCAGATGGGA CGTGGGCCCG GCCGGTTGGG 
AAGGTCGGTG GGGAGGTGAA GCTGTCTATC TCCTACAAAA ACAACAAACT CTTCATCATG 
GTGATGCATA TTCGGGGCTT GCAACCGCTC CAGGATGGCA ATGACCCTGA CCCCTATGTA 
AAGATTTACC TCCTTCCTGA CCCTCAGAAA ACCACAAAGA GGAAAACCAA GGTGGCCCGG 
AAAACCTGCA ACCCTACCTA CAATGAGATG CTGGTATATG ATGGGATCCC CAAGGGAGAC 
CTGCAGCAGC GGGAGCTACA GTTGAGTGTG CTGAGTGAGC AAGGATTTTG GGAGAATCTG 
CTTCTCGGGG AGGTGCACAT CCGCCTTCGG GAGCTGGACC TGGCCCAGGA GAAGACCGGC 
TGGTTTGGAC TGGGTTCTCG AGGTCATGGA ACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Phosphatidlyinositol-3-kinase C2 beta (PI3KC2?) is a potential new target to treat IgE mediated disease.
PloS one12(8)e0183474(2017)
Srivastava S,Li Z,Skolnik EY

PIK3C2B inhibition improves function and prolongs survival in myotubular myopathy animal models.
The Journal of clinical investigation126(9)3613-25(2016 Sep)
Sabha N,Volpatti JR,Gonorazky H,Reifler A,Davidson AE,Li X,Eltayeb NM,Dall'Armi C,Di Paolo G,Brooks SV,Buj-Bello A,Feldman EL,Dowling JJ

Role of protein kinase A and class II phosphatidylinositol 3-kinase C2? in the downregulation of KCa3.1 channel synthesis and membrane surface expression by lyso-globotriaosylceramide.
Biochemical and biophysical research communications470(4)907-12(2016 Feb)
Choi JY,Park S

Inactivation of the Class II PI3K-C2? Potentiates Insulin Signaling and Sensitivity.
Cell reports13(9)1881-94(2015 Dec)
Alliouachene S,Bilanges B,Chicanne G,Anderson KE,Pearce W,Ali K,Valet C,Posor Y,Low PC,Chaussade C,Scudamore CL,Salamon RS,Backer JM,Stephens L,Hawkins PT,Payrastre B,Vanhaesebroeck B

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Pik3c2b cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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