Mpdu1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mpdu1
Entrez Gene ID	24070
Full Name	mannose-P-dolichol utilization defect 1
Synonyms	LEC35,SL15,Supl15h
General protein information	Preferred Names mannose-P-dolichol utilization defect 1 Names mannose-P-dolichol utilization defect 1 protein suppressor of Lec15 and Lec35 glycosylation mutation homolog suppressor of Lec15 homolog (C.griseus)
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11 B3\|11 42.86 cM
Summary	This gene encodes a member of the PQ-loop superfamily. A similar gene in human encodes a protein that is required for monosaccharide-P-dolichol-dependent glycosyltransferase reactions, and disruption of this gene is the cause of congenital disorder of glycosylation (CDG) type 1F, a disease linked to defects in protein N-glycosylation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2014].

mRNA and Protein(s)

mRNA	Protein	Name
NM_011900.4	NP_036030.2	mannose-P-dolichol utilization defect 1 protein isoform 1
NM_001301711.1	NP_001288640.1	mannose-P-dolichol utilization defect 1 protein isoform 3
NM_001301710.1	NP_001288639.1	mannose-P-dolichol utilization defect 1 protein isoform 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	MPDU1	NP_004861.2
Rattus norvegicus (Norway rat)	Mpdu1	NP_001100481.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008375	XP_317078.3
Caenorhabditis elegans (roundworm)	CELE_F38E1.9	NP_505155.1
Bos taurus (cattle)	MPDU1	NP_001068647.1
Arabidopsis thaliana (thale cress)	AT5G59470	NP_200755.1
Mus musculus (house mouse)	Mpdu1	NP_036030.2
Neurospora crassa	NCU11327	XP_965266.2
Arabidopsis thaliana (thale cress)	AT4G07390	NP_567315.1
Pan troglodytes (chimpanzee)	MPDU1	XP_511960.1
Canis lupus familiaris (dog)	MPDU1	XP_546595.1
Drosophila melanogaster (fruit fly)	CG3792	NP_608889.1
	MPDU1	XP_001110171.1
Danio rerio (zebrafish)	mpdu1b	NP_001002130.1
Magnaporthe oryzae (rice blast fungus)	MGG_08600	XP_003716131.1
Oryza sativa (rice)	Os07g0479200	NP_001059633.1

Gene Ontology
Bibliography

Related articles in PubMed

MPDU1 mutations underlie a novel human congenital disorder of glycosylation, designated type If.
Schenk B, Imbach T, Frank CG, Grubenmann CE, Raymond GV, Hurvitz H, Korn-Lubetzki I, Revel-Vik S, Raas-Rotschild A, Luder AS, Jaeken J, Berger EG, Matthijs G, Hennet T, Aebi M
The Journal of clinical investigation108(11)1687-95(2001 Dec)

Requirement of the Lec35 gene for all known classes of monosaccharide-P-dolichol-dependent glycosyltransferase reactions in mammals.
Anand M, Rush JS, Ray S, Doucey MA, Weik J, Ware FE, Hofsteenge J, Waechter CJ, Lehrman MA
Molecular biology of the cell12(2)487-501(2001 Feb)

Five different genes, Eif4a1, Cd68, Supl15h, Sox15 and Fxr2h, are clustered in a 40 kb region of mouse chromosome 11.
Miyashita A, Shimizu N, Endo N, Hanyuu T, Ishii N, Ito K, Itoh Y, Shirai M, Nakajima T, Odani S, Kuwano R
Gene237(1)53-60(1999 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

study of mutations in human ortholog of MPDU1
Title: MPDU1 mutations underlie a novel human congenital disorder of glycosylation, designated type If.

A functional study of Mpdu1 in hamster
Title: Requirement of the Lec35 gene for all known classes of monosaccharide-P-dolichol-dependent glycosyltransferase reactions in mammals.

The following Mpdu1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mpdu1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu09118	NM_011900.4 Latest version!	Mus musculus mannose-P-dolichol utilization defect 1 (Mpdu1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu69182	NM_001301711.1 Latest version!	Mus musculus mannose-P-dolichol utilization defect 1 (Mpdu1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu69181	NM_001301710.1 Latest version!	Mus musculus mannose-P-dolichol utilization defect 1 (Mpdu1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mpdu1 ( NM_011900.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_036030.2 Mus musculus mannose-P-dolichol utilization defect 1 (Mpdu1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu09118
Clone ID Related Accession (Same CDS sequence)	NM_011900.4
Accession Version	NM_011900.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	mannose-P-dolichol utilization defect 1 protein isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL603707.5, AK080233.1 and BE686800.1. On Sep 11, 2014 this sequence version replaced NM_011900.3. Transcript Variant: This variant (1) encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: AK080233.1, AK089426.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164143 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGGGG AGGCCGACGG GCCGTTCAAA GGGCTGCTGG TGCCAATTCT TTTACCTGAG 
AAATGTTACG ACCAGCTCTT CGTGCAATGG GACTTGCTTC ATGTTCCCTG CCTCAAGATT 
CTCCTCAGCA AAGGCCTCGG GCTGGGCATC GTGGCTGGGT CACTTCTCGT CAAGCTGCCC 
CAGGTATTTA AACTCTTGGG AGCCAAGAGT GCAGAAGGAC TGAGTCTCCA GTCAGTAATG 
CTGGAGCTAG TGGCACTGAC CGGAACCGTG GTCTACAGCA TCACCAACAA CTTCCCCTTC 
AGCTCTTGGG GTGAAGCGCT GTTCCTGACA CTCCAGACCG TTGCCATCTG CTTCCTGGTC 
ATGCACTACA GAGGAGAGAC CGTGAAAGGA GTCGCTTTCC TTGCCTGCTA TGCCATGGTC 
CTGCTGGCGC TGCTCTCCCC GCTCACGCCT CTGGCTGTAG TCACTCTGCT CCAGGCCTCC 
AATGTACCTG CCGTGGTGGT GGGGAAGTTG CTTCAGGCAG CCACTAACTA CCGCAACGGA 
CACACAGGCC AGCTTTCAGC CATTACAGTG TTTATGCTGT TTGGGGGCTC CTTGGCCCGA 
ATCTTCACTT CTGTTCAGGA AACTGGAGAC CCCCTCATGG CTGGAGTCTT TGTGGTCTCT 
TCTCTCTGCA ATGGCCTCAT TGCTGCCCAG GTCCTCTTCT ACTGGAACGC AAAGGCTCCC 
CACAAACAGA AAAAGGAGCA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036030.2
CDS	79..822
Translation	MAGEADGPFKGLLVPILLPEKCYDQLFVQWDLLHVPCLKILLSKGLGLGIVAGSLLVKLPQVFKLLGAKSAEGLSLQSVMLELVALTGTVVYSITNNFPFSSWGEALFLTLQTVAICFLVMHYRGETVKGVAFLACYAMVLLALLSPLTPLAVVTLLQASNVPAVVVGKLLQAATNYRNGHTGQLSAITVFMLFGGSLARIFTSVQETGDPLMAGVFVVSSLCNGLIAAQVLFYWNAKAPHKQKKEQ

Target ORF information:

RefSeq Version	NM_011900.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus mannose-P-dolichol utilization defect 1 (Mpdu1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011900.4

ORF Insert Sequence:

ATGGCGGGGG AGGCCGACGG GCCGTTCAAA GGGCTGCTGG TGCCAATTCT TTTACCTGAG 
AAATGTTACG ACCAGCTCTT CGTGCAATGG GACTTGCTTC ATGTTCCCTG CCTCAAGATT 
CTCCTCAGCA AAGGCCTCGG GCTGGGCATC GTGGCTGGGT CACTTCTCGT CAAGCTGCCC 
CAGGTATTTA AACTCTTGGG AGCCAAGAGT GCAGAAGGAC TGAGTCTCCA GTCAGTAATG 
CTGGAGCTAG TGGCACTGAC CGGAACCGTG GTCTACAGCA TCACCAACAA CTTCCCCTTC 
AGCTCTTGGG GTGAAGCGCT GTTCCTGACA CTCCAGACCG TTGCCATCTG CTTCCTGGTC 
ATGCACTACA GAGGAGAGAC CGTGAAAGGA GTCGCTTTCC TTGCCTGCTA TGCCATGGTC 
CTGCTGGCGC TGCTCTCCCC GCTCACGCCT CTGGCTGTAG TCACTCTGCT CCAGGCCTCC 
AATGTACCTG CCGTGGTGGT GGGGAAGTTG CTTCAGGCAG CCACTAACTA CCGCAACGGA 
CACACAGGCC AGCTTTCAGC CATTACAGTG TTTATGCTGT TTGGGGGCTC CTTGGCCCGA 
ATCTTCACTT CTGTTCAGGA AACTGGAGAC CCCCTCATGG CTGGAGTCTT TGTGGTCTCT 
TCTCTCTGCA ATGGCCTCAT TGCTGCCCAG GTCCTCTTCT ACTGGAACGC AAAGGCTCCC 
CACAAACAGA AAAAGGAGCA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mpdu1 ( NM_001301711.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001288640.1 Mus musculus mannose-P-dolichol utilization defect 1 (Mpdu1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu69182
Clone ID Related Accession (Same CDS sequence)	NM_001301711.1
Accession Version	NM_001301711.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 384bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Mus musculus(house mouse)
Product	mannose-P-dolichol utilization defect 1 protein isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL603707.5, AK080233.1, CV561188.1 and BE686800.1. On Sep 11, 2014 this sequence version replaced XM_006533310.1. Transcript Variant: This variant (3) uses an alternate splice site and uses an in-frame downstream start codon, compared to variant 1. The resulting protein (isoform 3) has a shorter N-terminus than isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK172020.1, CK031333.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164137, SAMN01164143 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCACTACA GAGGAGAGAC CGTGAAAGGA GTCGCTTTCC TTGCCTGCTA TGCCATGGTC 
CTGCTGGCGC TGCTCTCCCC GCTCACGCCT CTGGCTGTAG TCACTCTGCT CCAGGCCTCC 
AATGTACCTG CCGTGGTGGT GGGGAAGTTG CTTCAGGCAG CCACTAACTA CCGCAACGGA 
CACACAGGCC AGCTTTCAGC CATTACAGTG TTTATGCTGT TTGGGGGCTC CTTGGCCCGA 
ATCTTCACTT CTGTTCAGGA AACTGGAGAC CCCCTCATGG CTGGAGTCTT TGTGGTCTCT 
TCTCTCTGCA ATGGCCTCAT TGCTGCCCAG GTCCTCTTCT ACTGGAACGC AAAGGCTCCC 
CACAAACAGA AAAAGGAGCA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001288640.1
CDS	474..857
Translation	MHYRGETVKGVAFLACYAMVLLALLSPLTPLAVVTLLQASNVPAVVVGKLLQAATNYRNGHTGQLSAITVFMLFGGSLARIFTSVQETGDPLMAGVFVVSSLCNGLIAAQVLFYWNAKAPHKQKKEQ

Target ORF information:

RefSeq Version	NM_001301711.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mannose-P-dolichol utilization defect 1 (Mpdu1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001301711.1

ORF Insert Sequence:

ATGCACTACA GAGGAGAGAC CGTGAAAGGA GTCGCTTTCC TTGCCTGCTA TGCCATGGTC 
CTGCTGGCGC TGCTCTCCCC GCTCACGCCT CTGGCTGTAG TCACTCTGCT CCAGGCCTCC 
AATGTACCTG CCGTGGTGGT GGGGAAGTTG CTTCAGGCAG CCACTAACTA CCGCAACGGA 
CACACAGGCC AGCTTTCAGC CATTACAGTG TTTATGCTGT TTGGGGGCTC CTTGGCCCGA 
ATCTTCACTT CTGTTCAGGA AACTGGAGAC CCCCTCATGG CTGGAGTCTT TGTGGTCTCT 
TCTCTCTGCA ATGGCCTCAT TGCTGCCCAG GTCCTCTTCT ACTGGAACGC AAAGGCTCCC 
CACAAACAGA AAAAGGAGCA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mpdu1 ( NM_001301710.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001288639.1 Mus musculus mannose-P-dolichol utilization defect 1 (Mpdu1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu69181
Clone ID Related Accession (Same CDS sequence)	NM_001301710.1
Accession Version	NM_001301710.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 600bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Mus musculus(house mouse)
Product	mannose-P-dolichol utilization defect 1 protein isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL603707.5, AK080233.1 and BE686800.1. Transcript Variant: This variant (2) lacks two alternate exons which results in a frameshift in the 3' coding region compared to variant 1. The resulting protein (isoform 2) has a distinct C-terminus and is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: CB588826.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164143 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGGGG AGGCCGACGG GCCGTTCAAA GGGCTGCTGG TGCCAATTCT TTTACCTGAG 
AAATGTTACG ACCAGCTCTT CGTGCAATGG GACTTGCTTC ATGTTCCCTG CCTCAAGATT 
CTCCTCAGCA AAGGCCTCGG GCTGGGCATC GTGGCTGGGT CACTTCTCGT CAAGCTGCCC 
CAGGTATTTA AACTCTTGGG AGCCAAGAGT GCAGAAGGAC TGAGTCTCCA GTCAGTAATG 
CTGGAGCTAG TGGCACTGAC CGGAACCGTG GTCTACAGCA TCACCAACAA CTTCCCCTTC 
AGTTGCTTCA GGCAGCCACT AACTACCGCA ACGGACACAC AGGCCAGCTT TCAGCCATTA 
CAGTGTTTAT GCTGTTTGGG GGCTCCTTGG CCCGAATCTT CACTTCTGTT CAGGAAACTG 
GAGACCCCCT CATGGCTGGA GTCTTTGTGG TCTCTTCTCT CTGCAATGGC CTCATTGCTG 
CCCAGGTCCT CTTCTACTGG AACGCAAAGG CTCCCCACAA ACAGAAAAAG GAGCAATAGA 
GCTGAGCTCG CTTCTAGAAC AATTCCATTT CCACTCATCC TCAGAGTCCT CCCCACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001288639.1
CDS	79..678
Translation	MAGEADGPFKGLLVPILLPEKCYDQLFVQWDLLHVPCLKILLSKGLGLGIVAGSLLVKLPQVFKLLGAKSAEGLSLQSVMLELVALTGTVVYSITNNFPFSCFRQPLTTATDTQASFQPLQCLCCLGAPWPESSLLFRKLETPSWLESLWSLLSAMASLLPRSSSTGTQRLPTNRKRSNRAELASRTIPFPLILRVLPT

Target ORF information:

RefSeq Version	NM_001301710.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mannose-P-dolichol utilization defect 1 (Mpdu1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001301710.1

ORF Insert Sequence:

ATGGCGGGGG AGGCCGACGG GCCGTTCAAA GGGCTGCTGG TGCCAATTCT TTTACCTGAG 
AAATGTTACG ACCAGCTCTT CGTGCAATGG GACTTGCTTC ATGTTCCCTG CCTCAAGATT 
CTCCTCAGCA AAGGCCTCGG GCTGGGCATC GTGGCTGGGT CACTTCTCGT CAAGCTGCCC 
CAGGTATTTA AACTCTTGGG AGCCAAGAGT GCAGAAGGAC TGAGTCTCCA GTCAGTAATG 
CTGGAGCTAG TGGCACTGAC CGGAACCGTG GTCTACAGCA TCACCAACAA CTTCCCCTTC 
AGTTGCTTCA GGCAGCCACT AACTACCGCA ACGGACACAC AGGCCAGCTT TCAGCCATTA 
CAGTGTTTAT GCTGTTTGGG GGCTCCTTGG CCCGAATCTT CACTTCTGTT CAGGAAACTG 
GAGACCCCCT CATGGCTGGA GTCTTTGTGG TCTCTTCTCT CTGCAATGGC CTCATTGCTG 
CCCAGGTCCT CTTCTACTGG AACGCAAAGG CTCCCCACAA ACAGAAAAAG GAGCAATAGA 
GCTGAGCTCG CTTCTAGAAC AATTCCATTT CCACTCATCC TCAGAGTCCT CCCCACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MPDU1 mutations underlie a novel human congenital disorder of glycosylation, designated type If.
The Journal of clinical investigation108(11)1687-95(2001 Dec)
Schenk B,Imbach T,Frank CG,Grubenmann CE,Raymond GV,Hurvitz H,Korn-Lubetzki I,Revel-Vik S,Raas-Rotschild A,Luder AS,Jaeken J,Berger EG,Matthijs G,Hennet T,Aebi M

Requirement of the Lec35 gene for all known classes of monosaccharide-P-dolichol-dependent glycosyltransferase reactions in mammals.
Molecular biology of the cell12(2)487-501(2001 Feb)
Anand M,Rush JS,Ray S,Doucey MA,Weik J,Ware FE,Hofsteenge J,Waechter CJ,Lehrman MA

Five different genes, Eif4a1, Cd68, Supl15h, Sox15 and Fxr2h, are clustered in a 40 kb region of mouse chromosome 11.
Gene237(1)53-60(1999 Sep)
Miyashita A,Shimizu N,Endo N,Hanyuu T,Ishii N,Ito K,Itoh Y,Shirai M,Nakajima T,Odani S,Kuwano R

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

Mpdu1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報