Sh3bp2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sh3bp2
Entrez Gene ID	24055
Full Name	SH3-domain binding protein 2
Synonyms	3BP2
General protein information	Preferred Names SH3-domain binding protein 2 Names SH3 domain-binding protein 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 B2

mRNA and Protein(s)

mRNA	Protein	Name
XM_006503955.1	XP_006504018.1	SH3 domain-binding protein 2 isoform X1
NM_011893.3	NP_036023.3	SH3 domain-binding protein 2 isoform a
NM_001145858.1	NP_001139330.1	SH3 domain-binding protein 2 isoform c
XM_006503954.2	XP_006504017.1	SH3 domain-binding protein 2 isoform X1
XM_006503954.1	XP_006504017.1	SH3 domain-binding protein 2 isoform X1
NM_001145859.1	NP_001139331.1	SH3 domain-binding protein 2 isoform b

Pathways from BioSystems

Homologies

Homo sapiens (human)	SH3BP2	NP_001139328.1
Mus musculus (house mouse)	Sh3bp2	NP_001139331.1
Rattus norvegicus (Norway rat)	Sh3bp2	NP_001094154.1
Gallus gallus (chicken)	SH3BP2	NP_001026336.1
Canis lupus familiaris (dog)	SH3BP2	XP_005618564.1
Pan troglodytes (chimpanzee)	LOC750514	XP_003950326.1
	SH3BP2	XP_001088909.2
Bos taurus (cattle)	SH3BP2	NP_001178455.1

Gene Ontology
Bibliography

Related articles in PubMed

RANKL coordinates multiple osteoclastogenic pathways by regulating expression of ubiquitin ligase RNF146.
Matsumoto Y, Larose J, Kent OA, Lim M, Changoor A, Zhang L, Storozhuk Y, Mao X, Grynpas MD, Cong F, Rottapel R
The Journal of clinical investigation127(4)1303-1315(2017 Apr)

Reciprocal stabilization of ABL and TAZ regulates osteoblastogenesis through transcription factor RUNX2.
Matsumoto Y, La Rose J, Kent OA, Wagner MJ, Narimatsu M, Levy AD, Omar MH, Tong J, Krieger JR, Riggs E, Storozhuk Y, Pasquale J, Ventura M, Yeganeh B, Post M, Moran MF, Grynpas MD, Wrana JL, Superti-Furga G, Koleske AJ, Pendergast AM, Rottapel R
The Journal of clinical investigation126(12)4482-4496(2016 Dec)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

3BP2 was needed for LPS-induced activation of macrophage signaling pathways. Having a single Sh3bp2 Gly418Arg allele and PAMP stimulation had a cooperative effect on macrophage activation and inflammatory responses.
Title: Cherubism allele heterozygosity amplifies microbe-induced inflammatory responses in murine macrophages.

Bone marrow transplantation also ameliorated the inflammation and bone loss in 10-week-old Sh3bp2(knock in) mice.
Title: Bone marrow transplantation improves autoinflammation and inflammatory bone loss in SH3BP2 knock-in cherubism mice.

Phosphorylation of SH3BP2 at Y183 is critical for the TNF-alpha production.
Title: Enhanced TLR-MYD88 signaling stimulates autoinflammation in SH3BP2 cherubism mice and defines the etiology of cherubism.

Gain-of-function of SH3BP2 augments inflammation and bone loss in the CIA model through increased macrophage activation and osteoclast formation.
Title: SH3BP2 gain-of-function mutation exacerbates inflammation and bone loss in a murine collagen-induced arthritis model.

Etanercept administration to neonatal SH3BP2 knock-in cherubism mice prevents TNF-alpha-induced inflammation and bone loss.
Title: Etanercept administration to neonatal SH3BP2 knock-in cherubism mice prevents TNF-?-induced inflammation and bone loss.

The following Sh3bp2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sh3bp2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu09104	NM_011893.3 Latest version!	Mus musculus SH3-domain binding protein 2 (Sh3bp2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OMu09104	XM_006503955.1 Latest version!	Mus musculus SH3-domain binding protein 2 (Sh3bp2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OMu06988	NM_001145858.1 Latest version!	Mus musculus SH3-domain binding protein 2 (Sh3bp2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$349.30 $499.00
OMu06990	NM_001145859.1 Latest version!	Mus musculus SH3-domain binding protein 2 (Sh3bp2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu09104	XM_006503954.2 Latest version!	Mus musculus SH3-domain binding protein 2 (Sh3bp2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu09104	XM_006503954.1	Mus musculus SH3-domain binding protein 2 (Sh3bp2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sh3bp2 ( NM_011893.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_036023.3 Mus musculus SH3-domain binding protein 2 (Sh3bp2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu09104
Clone ID Related Accession (Same CDS sequence)	XM_006503955.1 , NM_011893.3 , XM_006503954.2 , XM_006503954.1
Accession Version	NM_011893.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1680bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	SH3 domain-binding protein 2 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK155898.1 and BC085497.1. On Mar 14, 2009 this sequence version replaced NM_011893.2. Transcript Variant: This variant (1) represents use of an alternative promoter and 5' UTR and uses a downstream translation start site, compared to variant 2. The resulting isoform (a) has a shorter N-terminus, compared to isoform b. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK155898.1, AK155103.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCTG AGGAGATGCA GTGGCCTGTC CCCATGAAGG CCATTGGTGC CCAGAATTTG 
CTGACCATGC CTGGTGGTGT GGCGAAGGCT GGTTACCTGC ATAAGAAGGG TGGCACCCAA 
CTGCAGCTGC TCAAATGGCC CCTCCGCTTT GTGATCATCC ACAAGCGCTG CATCTACTAC 
TTCAAGAGCA GCACATCTGC CTCCCCACAG GGTGCCTTCT CACTGAGTGG CTACAACAGA 
GTGATGCGAG CAGCTGAGGA GACCACGTCC AACAATGTCT TCCCATTCAA GATCATTCAC 
ATCAGCAAGA AGCACCGAAC ATGGTTCTTC TCAGCGTCCT CTGAGGATGA GCGCAAGAGC 
TGGATGGCCT TGCTACGCAG GGAAATTGGC CACTTCCATG AGAAGAAGGA ACTACCTCTA 
GACACCAGCG ACTCTAGCTC AGACACGGAC AGCTTCTATG GTGCAGTTGA GCGACCTATA 
GATATCAGCC TGTCTTCATA TCCCATGGAC AATGAAGATT ACGAACATGA AGATGAGGAC 
GACTCGTACC TGGAGCCTGA TTCCCCGGGG CCCATGAAGC TTGAAGATGC CCTGACCTAT 
CCACCAGCCT ACCCACCACC CCCTGTGCCT GTACCTCGGA AGCCAGCCTT CTCTGACTTG 
CCCCGTGCCC ACTCGTTCAC CTCTAAAAGT CCAAGTCCCT TACTGCCACC CCCACCTCCT 
AAGCGAGGCC TTCCAGACAC TGGCTCTGCC CCTGAGGATG CTAAGGACGC ATTGGGCCTG 
AGGCGAGTAG AGCCTGGCCT CAGGGTGCCT GCCACCCCTC GGAGGATGAG TGACCCCCCA 
ATGAGCAATG TACCCACCGT GCCCAACCTC CGGAAACATC CTTGCTTCCG GGACAGTGTG 
AATCCCGGCC TAGAACCCTG GACCCCTGGC CATGGGACTA GCTCTGTCTC CAGCTCTACC 
ACCATGGCCG TTGCCACCTC TAGGAACTGT GACAAACTCA AGTCATTCCA CCTGTCTTCC 
CGAGGGCCAC CCACATCTGA GCCCCCACCC GTGCCTGCTA ATAAGCCCAA GTTCCTGAAG 
ATAGCTGAAG AACCTTCCCC GAGGGAGGCA GCCAAGTTTG CACCTGTGCC CCCTGTGGCC 
CCCAGGCCTC CTGTGCAGAA GATGCCCATG CCTGAGGCTA CTGTTCGGCC TGCAGTGCTG 
CCTAGACCAG AGAACACACC TCTTCCTCAC CTCCAGCGAT CCCCCCCTGA TGGACAGAGT 
TTCAGGGGCT TCTCCTTTGA AAAGGCCCGC CAGCCCTCAC AGGCTGACAC TGGCGAGGAG 
GACTCAGATG AAGACTATGA GAAGGTGCCG CTGCCTAACT CGGTGTTTGT CAACACGACA 
GAGTCCTGCG AGGTGGAAAG GTTGTTCAAA GCTACGGACC CCCGTGGAGA GCCTCAGGAT 
GGGCTGTATT GCATTCGGAA CTCCTCTACC AAGTCAGGGA AGGTTTTGGT CGTGTGGGAT 
GAATCTTCTA ACAAAGTGAG GAATTATCGC ATCTTTGAAA AGGATTCTAA GTTCTACCTG 
GAAGGTGAGG TTCTGTTTGC CAGTGTGGGC AGCATGGTGG AGCACTACCA CACCCATGTG 
CTGCCCAGCC ATCAGAGCCT GTTGCTGCGG CACCCATACG GCTACGCTGG GCCCAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036023.3
CDS	123..1802
Translation	MAAEEMQWPVPMKAIGAQNLLTMPGGVAKAGYLHKKGGTQLQLLKWPLRFVIIHKRCIYYFKSSTSASPQGAFSLSGYNRVMRAAEETTSNNVFPFKIIHISKKHRTWFFSASSEDERKSWMALLRREIGHFHEKKELPLDTSDSSSDTDSFYGAVERPIDISLSSYPMDNEDYEHEDEDDSYLEPDSPGPMKLEDALTYPPAYPPPPVPVPRKPAFSDLPRAHSFTSKSPSPLLPPPPPKRGLPDTGSAPEDAKDALGLRRVEPGLRVPATPRRMSDPPMSNVPTVPNLRKHPCFRDSVNPGLEPWTPGHGTSSVSSSTTMAVATSRNCDKLKSFHLSSRGPPTSEPPPVPANKPKFLKIAEEPSPREAAKFAPVPPVAPRPPVQKMPMPEATVRPAVLPRPENTPLPHLQRSPPDGQSFRGFSFEKARQPSQADTGEEDSDEDYEKVPLPNSVFVNTTESCEVERLFKATDPRGEPQDGLYCIRNSSTKSGKVLVVWDESSNKVRNYRIFEKDSKFYLEGEVLFASVGSMVEHYHTHVLPSHQSLLLRHPYGYAGPR

Target ORF information:

RefSeq Version	NM_011893.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus SH3-domain binding protein 2 (Sh3bp2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011893.3

ORF Insert Sequence:

ATGGCGGCTG AGGAGATGCA GTGGCCTGTC CCCATGAAGG CCATTGGTGC CCAGAATTTG 
CTGACCATGC CTGGTGGTGT GGCGAAGGCT GGTTACCTGC ATAAGAAGGG TGGCACCCAA 
CTGCAGCTGC TCAAATGGCC CCTCCGCTTT GTGATCATCC ACAAGCGCTG CATCTACTAC 
TTCAAGAGCA GCACATCTGC CTCCCCACAG GGTGCCTTCT CACTGAGTGG CTACAACAGA 
GTGATGCGAG CAGCTGAGGA GACCACGTCC AACAATGTCT TCCCATTCAA GATCATTCAC 
ATCAGCAAGA AGCACCGAAC ATGGTTCTTC TCAGCGTCCT CTGAGGATGA GCGCAAGAGC 
TGGATGGCCT TGCTACGCAG GGAAATTGGC CACTTCCATG AGAAGAAGGA ACTACCTCTA 
GACACCAGCG ACTCTAGCTC AGACACGGAC AGCTTCTATG GTGCAGTTGA GCGACCTATA 
GATATCAGCC TGTCTTCATA TCCCATGGAC AATGAAGATT ACGAACATGA AGATGAGGAC 
GACTCGTACC TGGAGCCTGA TTCCCCGGGG CCCATGAAGC TTGAAGATGC CCTGACCTAT 
CCACCAGCCT ACCCACCACC CCCTGTGCCT GTACCTCGGA AGCCAGCCTT CTCTGACTTG 
CCCCGTGCCC ACTCGTTCAC CTCTAAAAGT CCAAGTCCCT TACTGCCACC CCCACCTCCT 
AAGCGAGGCC TTCCAGACAC TGGCTCTGCC CCTGAGGATG CTAAGGACGC ATTGGGCCTG 
AGGCGAGTAG AGCCTGGCCT CAGGGTGCCT GCCACCCCTC GGAGGATGAG TGACCCCCCA 
ATGAGCAATG TACCCACCGT GCCCAACCTC CGGAAACATC CTTGCTTCCG GGACAGTGTG 
AATCCCGGCC TAGAACCCTG GACCCCTGGC CATGGGACTA GCTCTGTCTC CAGCTCTACC 
ACCATGGCCG TTGCCACCTC TAGGAACTGT GACAAACTCA AGTCATTCCA CCTGTCTTCC 
CGAGGGCCAC CCACATCTGA GCCCCCACCC GTGCCTGCTA ATAAGCCCAA GTTCCTGAAG 
ATAGCTGAAG AACCTTCCCC GAGGGAGGCA GCCAAGTTTG CACCTGTGCC CCCTGTGGCC 
CCCAGGCCTC CTGTGCAGAA GATGCCCATG CCTGAGGCTA CTGTTCGGCC TGCAGTGCTG 
CCTAGACCAG AGAACACACC TCTTCCTCAC CTCCAGCGAT CCCCCCCTGA TGGACAGAGT 
TTCAGGGGCT TCTCCTTTGA AAAGGCCCGC CAGCCCTCAC AGGCTGACAC TGGCGAGGAG 
GACTCAGATG AAGACTATGA GAAGGTGCCG CTGCCTAACT CGGTGTTTGT CAACACGACA 
GAGTCCTGCG AGGTGGAAAG GTTGTTCAAA GCTACGGACC CCCGTGGAGA GCCTCAGGAT 
GGGCTGTATT GCATTCGGAA CTCCTCTACC AAGTCAGGGA AGGTTTTGGT CGTGTGGGAT 
GAATCTTCTA ACAAAGTGAG GAATTATCGC ATCTTTGAAA AGGATTCTAA GTTCTACCTG 
GAAGGTGAGG TTCTGTTTGC CAGTGTGGGC AGCATGGTGG AGCACTACCA CACCCATGTG 
CTGCCCAGCC ATCAGAGCCT GTTGCTGCGG CACCCATACG GCTACGCTGG GCCCAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sh3bp2 ( XM_006503955.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006504018.1 Mus musculus SH3-domain binding protein 2 (Sh3bp2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu09104
Clone ID Related Accession (Same CDS sequence)	XM_006503955.1 , NM_011893.3 , XM_006503954.2 , XM_006503954.1
Accession Version	XM_006503955.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1680bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	SH3 domain-binding protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTG AGGAGATGCA GTGGCCTGTC CCCATGAAGG CCATTGGTGC CCAGAATTTG 
CTGACCATGC CTGGTGGTGT GGCGAAGGCT GGTTACCTGC ATAAGAAGGG TGGCACCCAA 
CTGCAGCTGC TCAAATGGCC CCTCCGCTTT GTGATCATCC ACAAGCGCTG CATCTACTAC 
TTCAAGAGCA GCACATCTGC CTCCCCACAG GGTGCCTTCT CACTGAGTGG CTACAACAGA 
GTGATGCGAG CAGCTGAGGA GACCACGTCC AACAATGTCT TCCCATTCAA GATCATTCAC 
ATCAGCAAGA AGCACCGAAC ATGGTTCTTC TCAGCGTCCT CTGAGGATGA GCGCAAGAGC 
TGGATGGCCT TGCTACGCAG GGAAATTGGC CACTTCCATG AGAAGAAGGA ACTACCTCTA 
GACACCAGCG ACTCTAGCTC AGACACGGAC AGCTTCTATG GTGCAGTTGA GCGACCTATA 
GATATCAGCC TGTCTTCATA TCCCATGGAC AATGAAGATT ACGAACATGA AGATGAGGAC 
GACTCGTACC TGGAGCCTGA TTCCCCGGGG CCCATGAAGC TTGAAGATGC CCTGACCTAT 
CCACCAGCCT ACCCACCACC CCCTGTGCCT GTACCTCGGA AGCCAGCCTT CTCTGACTTG 
CCCCGTGCCC ACTCGTTCAC CTCTAAAAGT CCAAGTCCCT TACTGCCACC CCCACCTCCT 
AAGCGAGGCC TTCCAGACAC TGGCTCTGCC CCTGAGGATG CTAAGGACGC ATTGGGCCTG 
AGGCGAGTAG AGCCTGGCCT CAGGGTGCCT GCCACCCCTC GGAGGATGAG TGACCCCCCA 
ATGAGCAATG TACCCACCGT GCCCAACCTC CGGAAACATC CTTGCTTCCG GGACAGTGTG 
AATCCCGGCC TAGAACCCTG GACCCCTGGC CATGGGACTA GCTCTGTCTC CAGCTCTACC 
ACCATGGCCG TTGCCACCTC TAGGAACTGT GACAAACTCA AGTCATTCCA CCTGTCTTCC 
CGAGGGCCAC CCACATCTGA GCCCCCACCC GTGCCTGCTA ATAAGCCCAA GTTCCTGAAG 
ATAGCTGAAG AACCTTCCCC GAGGGAGGCA GCCAAGTTTG CACCTGTGCC CCCTGTGGCC 
CCCAGGCCTC CTGTGCAGAA GATGCCCATG CCTGAGGCTA CTGTTCGGCC TGCAGTGCTG 
CCTAGACCAG AGAACACACC TCTTCCTCAC CTCCAGCGAT CCCCCCCTGA TGGACAGAGT 
TTCAGGGGCT TCTCCTTTGA AAAGGCCCGC CAGCCCTCAC AGGCTGACAC TGGCGAGGAG 
GACTCAGATG AAGACTATGA GAAGGTGCCG CTGCCTAACT CGGTGTTTGT CAACACGACA 
GAGTCCTGCG AGGTGGAAAG GTTGTTCAAA GCTACGGACC CCCGTGGAGA GCCTCAGGAT 
GGGCTGTATT GCATTCGGAA CTCCTCTACC AAGTCAGGGA AGGTTTTGGT CGTGTGGGAT 
GAATCTTCTA ACAAAGTGAG GAATTATCGC ATCTTTGAAA AGGATTCTAA GTTCTACCTG 
GAAGGTGAGG TTCTGTTTGC CAGTGTGGGC AGCATGGTGG AGCACTACCA CACCCATGTG 
CTGCCCAGCC ATCAGAGCCT GTTGCTGCGG CACCCATACG GCTACGCTGG GCCCAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006504018.1
CDS	63..1742
Translation	MAAEEMQWPVPMKAIGAQNLLTMPGGVAKAGYLHKKGGTQLQLLKWPLRFVIIHKRCIYYFKSSTSASPQGAFSLSGYNRVMRAAEETTSNNVFPFKIIHISKKHRTWFFSASSEDERKSWMALLRREIGHFHEKKELPLDTSDSSSDTDSFYGAVERPIDISLSSYPMDNEDYEHEDEDDSYLEPDSPGPMKLEDALTYPPAYPPPPVPVPRKPAFSDLPRAHSFTSKSPSPLLPPPPPKRGLPDTGSAPEDAKDALGLRRVEPGLRVPATPRRMSDPPMSNVPTVPNLRKHPCFRDSVNPGLEPWTPGHGTSSVSSSTTMAVATSRNCDKLKSFHLSSRGPPTSEPPPVPANKPKFLKIAEEPSPREAAKFAPVPPVAPRPPVQKMPMPEATVRPAVLPRPENTPLPHLQRSPPDGQSFRGFSFEKARQPSQADTGEEDSDEDYEKVPLPNSVFVNTTESCEVERLFKATDPRGEPQDGLYCIRNSSTKSGKVLVVWDESSNKVRNYRIFEKDSKFYLEGEVLFASVGSMVEHYHTHVLPSHQSLLLRHPYGYAGPR

Target ORF information:

RefSeq Version	XM_006503955.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus SH3-domain binding protein 2 (Sh3bp2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006503955.1

ORF Insert Sequence:

ATGGCGGCTG AGGAGATGCA GTGGCCTGTC CCCATGAAGG CCATTGGTGC CCAGAATTTG 
CTGACCATGC CTGGTGGTGT GGCGAAGGCT GGTTACCTGC ATAAGAAGGG TGGCACCCAA 
CTGCAGCTGC TCAAATGGCC CCTCCGCTTT GTGATCATCC ACAAGCGCTG CATCTACTAC 
TTCAAGAGCA GCACATCTGC CTCCCCACAG GGTGCCTTCT CACTGAGTGG CTACAACAGA 
GTGATGCGAG CAGCTGAGGA GACCACGTCC AACAATGTCT TCCCATTCAA GATCATTCAC 
ATCAGCAAGA AGCACCGAAC ATGGTTCTTC TCAGCGTCCT CTGAGGATGA GCGCAAGAGC 
TGGATGGCCT TGCTACGCAG GGAAATTGGC CACTTCCATG AGAAGAAGGA ACTACCTCTA 
GACACCAGCG ACTCTAGCTC AGACACGGAC AGCTTCTATG GTGCAGTTGA GCGACCTATA 
GATATCAGCC TGTCTTCATA TCCCATGGAC AATGAAGATT ACGAACATGA AGATGAGGAC 
GACTCGTACC TGGAGCCTGA TTCCCCGGGG CCCATGAAGC TTGAAGATGC CCTGACCTAT 
CCACCAGCCT ACCCACCACC CCCTGTGCCT GTACCTCGGA AGCCAGCCTT CTCTGACTTG 
CCCCGTGCCC ACTCGTTCAC CTCTAAAAGT CCAAGTCCCT TACTGCCACC CCCACCTCCT 
AAGCGAGGCC TTCCAGACAC TGGCTCTGCC CCTGAGGATG CTAAGGACGC ATTGGGCCTG 
AGGCGAGTAG AGCCTGGCCT CAGGGTGCCT GCCACCCCTC GGAGGATGAG TGACCCCCCA 
ATGAGCAATG TACCCACCGT GCCCAACCTC CGGAAACATC CTTGCTTCCG GGACAGTGTG 
AATCCCGGCC TAGAACCCTG GACCCCTGGC CATGGGACTA GCTCTGTCTC CAGCTCTACC 
ACCATGGCCG TTGCCACCTC TAGGAACTGT GACAAACTCA AGTCATTCCA CCTGTCTTCC 
CGAGGGCCAC CCACATCTGA GCCCCCACCC GTGCCTGCTA ATAAGCCCAA GTTCCTGAAG 
ATAGCTGAAG AACCTTCCCC GAGGGAGGCA GCCAAGTTTG CACCTGTGCC CCCTGTGGCC 
CCCAGGCCTC CTGTGCAGAA GATGCCCATG CCTGAGGCTA CTGTTCGGCC TGCAGTGCTG 
CCTAGACCAG AGAACACACC TCTTCCTCAC CTCCAGCGAT CCCCCCCTGA TGGACAGAGT 
TTCAGGGGCT TCTCCTTTGA AAAGGCCCGC CAGCCCTCAC AGGCTGACAC TGGCGAGGAG 
GACTCAGATG AAGACTATGA GAAGGTGCCG CTGCCTAACT CGGTGTTTGT CAACACGACA 
GAGTCCTGCG AGGTGGAAAG GTTGTTCAAA GCTACGGACC CCCGTGGAGA GCCTCAGGAT 
GGGCTGTATT GCATTCGGAA CTCCTCTACC AAGTCAGGGA AGGTTTTGGT CGTGTGGGAT 
GAATCTTCTA ACAAAGTGAG GAATTATCGC ATCTTTGAAA AGGATTCTAA GTTCTACCTG 
GAAGGTGAGG TTCTGTTTGC CAGTGTGGGC AGCATGGTGG AGCACTACCA CACCCATGTG 
CTGCCCAGCC ATCAGAGCCT GTTGCTGCGG CACCCATACG GCTACGCTGG GCCCAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sh3bp2 ( XM_006503954.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006504017.1 Mus musculus SH3-domain binding protein 2 (Sh3bp2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu09104
Clone ID Related Accession (Same CDS sequence)	XM_006503955.1 , NM_011893.3 , XM_006503954.2 , XM_006503954.1
Accession Version	XM_006503954.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1680bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	SH3 domain-binding protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTG AGGAGATGCA GTGGCCTGTC CCCATGAAGG CCATTGGTGC CCAGAATTTG 
CTGACCATGC CTGGTGGTGT GGCGAAGGCT GGTTACCTGC ATAAGAAGGG TGGCACCCAA 
CTGCAGCTGC TCAAATGGCC CCTCCGCTTT GTGATCATCC ACAAGCGCTG CATCTACTAC 
TTCAAGAGCA GCACATCTGC CTCCCCACAG GGTGCCTTCT CACTGAGTGG CTACAACAGA 
GTGATGCGAG CAGCTGAGGA GACCACGTCC AACAATGTCT TCCCATTCAA GATCATTCAC 
ATCAGCAAGA AGCACCGAAC ATGGTTCTTC TCAGCGTCCT CTGAGGATGA GCGCAAGAGC 
TGGATGGCCT TGCTACGCAG GGAAATTGGC CACTTCCATG AGAAGAAGGA ACTACCTCTA 
GACACCAGCG ACTCTAGCTC AGACACGGAC AGCTTCTATG GTGCAGTTGA GCGACCTATA 
GATATCAGCC TGTCTTCATA TCCCATGGAC AATGAAGATT ACGAACATGA AGATGAGGAC 
GACTCGTACC TGGAGCCTGA TTCCCCGGGG CCCATGAAGC TTGAAGATGC CCTGACCTAT 
CCACCAGCCT ACCCACCACC CCCTGTGCCT GTACCTCGGA AGCCAGCCTT CTCTGACTTG 
CCCCGTGCCC ACTCGTTCAC CTCTAAAAGT CCAAGTCCCT TACTGCCACC CCCACCTCCT 
AAGCGAGGCC TTCCAGACAC TGGCTCTGCC CCTGAGGATG CTAAGGACGC ATTGGGCCTG 
AGGCGAGTAG AGCCTGGCCT CAGGGTGCCT GCCACCCCTC GGAGGATGAG TGACCCCCCA 
ATGAGCAATG TACCCACCGT GCCCAACCTC CGGAAACATC CTTGCTTCCG GGACAGTGTG 
AATCCCGGCC TAGAACCCTG GACCCCTGGC CATGGGACTA GCTCTGTCTC CAGCTCTACC 
ACCATGGCCG TTGCCACCTC TAGGAACTGT GACAAACTCA AGTCATTCCA CCTGTCTTCC 
CGAGGGCCAC CCACATCTGA GCCCCCACCC GTGCCTGCTA ATAAGCCCAA GTTCCTGAAG 
ATAGCTGAAG AACCTTCCCC GAGGGAGGCA GCCAAGTTTG CACCTGTGCC CCCTGTGGCC 
CCCAGGCCTC CTGTGCAGAA GATGCCCATG CCTGAGGCTA CTGTTCGGCC TGCAGTGCTG 
CCTAGACCAG AGAACACACC TCTTCCTCAC CTCCAGCGAT CCCCCCCTGA TGGACAGAGT 
TTCAGGGGCT TCTCCTTTGA AAAGGCCCGC CAGCCCTCAC AGGCTGACAC TGGCGAGGAG 
GACTCAGATG AAGACTATGA GAAGGTGCCG CTGCCTAACT CGGTGTTTGT CAACACGACA 
GAGTCCTGCG AGGTGGAAAG GTTGTTCAAA GCTACGGACC CCCGTGGAGA GCCTCAGGAT 
GGGCTGTATT GCATTCGGAA CTCCTCTACC AAGTCAGGGA AGGTTTTGGT CGTGTGGGAT 
GAATCTTCTA ACAAAGTGAG GAATTATCGC ATCTTTGAAA AGGATTCTAA GTTCTACCTG 
GAAGGTGAGG TTCTGTTTGC CAGTGTGGGC AGCATGGTGG AGCACTACCA CACCCATGTG 
CTGCCCAGCC ATCAGAGCCT GTTGCTGCGG CACCCATACG GCTACGCTGG GCCCAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006504017.1
CDS	28..1707
Translation	MAAEEMQWPVPMKAIGAQNLLTMPGGVAKAGYLHKKGGTQLQLLKWPLRFVIIHKRCIYYFKSSTSASPQGAFSLSGYNRVMRAAEETTSNNVFPFKIIHISKKHRTWFFSASSEDERKSWMALLRREIGHFHEKKELPLDTSDSSSDTDSFYGAVERPIDISLSSYPMDNEDYEHEDEDDSYLEPDSPGPMKLEDALTYPPAYPPPPVPVPRKPAFSDLPRAHSFTSKSPSPLLPPPPPKRGLPDTGSAPEDAKDALGLRRVEPGLRVPATPRRMSDPPMSNVPTVPNLRKHPCFRDSVNPGLEPWTPGHGTSSVSSSTTMAVATSRNCDKLKSFHLSSRGPPTSEPPPVPANKPKFLKIAEEPSPREAAKFAPVPPVAPRPPVQKMPMPEATVRPAVLPRPENTPLPHLQRSPPDGQSFRGFSFEKARQPSQADTGEEDSDEDYEKVPLPNSVFVNTTESCEVERLFKATDPRGEPQDGLYCIRNSSTKSGKVLVVWDESSNKVRNYRIFEKDSKFYLEGEVLFASVGSMVEHYHTHVLPSHQSLLLRHPYGYAGPR

Target ORF information:

RefSeq Version	XM_006503954.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus SH3-domain binding protein 2 (Sh3bp2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006503954.1

ORF Insert Sequence:

ATGGCGGCTG AGGAGATGCA GTGGCCTGTC CCCATGAAGG CCATTGGTGC CCAGAATTTG 
CTGACCATGC CTGGTGGTGT GGCGAAGGCT GGTTACCTGC ATAAGAAGGG TGGCACCCAA 
CTGCAGCTGC TCAAATGGCC CCTCCGCTTT GTGATCATCC ACAAGCGCTG CATCTACTAC 
TTCAAGAGCA GCACATCTGC CTCCCCACAG GGTGCCTTCT CACTGAGTGG CTACAACAGA 
GTGATGCGAG CAGCTGAGGA GACCACGTCC AACAATGTCT TCCCATTCAA GATCATTCAC 
ATCAGCAAGA AGCACCGAAC ATGGTTCTTC TCAGCGTCCT CTGAGGATGA GCGCAAGAGC 
TGGATGGCCT TGCTACGCAG GGAAATTGGC CACTTCCATG AGAAGAAGGA ACTACCTCTA 
GACACCAGCG ACTCTAGCTC AGACACGGAC AGCTTCTATG GTGCAGTTGA GCGACCTATA 
GATATCAGCC TGTCTTCATA TCCCATGGAC AATGAAGATT ACGAACATGA AGATGAGGAC 
GACTCGTACC TGGAGCCTGA TTCCCCGGGG CCCATGAAGC TTGAAGATGC CCTGACCTAT 
CCACCAGCCT ACCCACCACC CCCTGTGCCT GTACCTCGGA AGCCAGCCTT CTCTGACTTG 
CCCCGTGCCC ACTCGTTCAC CTCTAAAAGT CCAAGTCCCT TACTGCCACC CCCACCTCCT 
AAGCGAGGCC TTCCAGACAC TGGCTCTGCC CCTGAGGATG CTAAGGACGC ATTGGGCCTG 
AGGCGAGTAG AGCCTGGCCT CAGGGTGCCT GCCACCCCTC GGAGGATGAG TGACCCCCCA 
ATGAGCAATG TACCCACCGT GCCCAACCTC CGGAAACATC CTTGCTTCCG GGACAGTGTG 
AATCCCGGCC TAGAACCCTG GACCCCTGGC CATGGGACTA GCTCTGTCTC CAGCTCTACC 
ACCATGGCCG TTGCCACCTC TAGGAACTGT GACAAACTCA AGTCATTCCA CCTGTCTTCC 
CGAGGGCCAC CCACATCTGA GCCCCCACCC GTGCCTGCTA ATAAGCCCAA GTTCCTGAAG 
ATAGCTGAAG AACCTTCCCC GAGGGAGGCA GCCAAGTTTG CACCTGTGCC CCCTGTGGCC 
CCCAGGCCTC CTGTGCAGAA GATGCCCATG CCTGAGGCTA CTGTTCGGCC TGCAGTGCTG 
CCTAGACCAG AGAACACACC TCTTCCTCAC CTCCAGCGAT CCCCCCCTGA TGGACAGAGT 
TTCAGGGGCT TCTCCTTTGA AAAGGCCCGC CAGCCCTCAC AGGCTGACAC TGGCGAGGAG 
GACTCAGATG AAGACTATGA GAAGGTGCCG CTGCCTAACT CGGTGTTTGT CAACACGACA 
GAGTCCTGCG AGGTGGAAAG GTTGTTCAAA GCTACGGACC CCCGTGGAGA GCCTCAGGAT 
GGGCTGTATT GCATTCGGAA CTCCTCTACC AAGTCAGGGA AGGTTTTGGT CGTGTGGGAT 
GAATCTTCTA ACAAAGTGAG GAATTATCGC ATCTTTGAAA AGGATTCTAA GTTCTACCTG 
GAAGGTGAGG TTCTGTTTGC CAGTGTGGGC AGCATGGTGG AGCACTACCA CACCCATGTG 
CTGCCCAGCC ATCAGAGCCT GTTGCTGCGG CACCCATACG GCTACGCTGG GCCCAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sh3bp2 ( NM_001145858.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001139330.1 Mus musculus SH3-domain binding protein 2 (Sh3bp2), transcript variant 3, mRNA.

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CloneID	OMu06988
Clone ID Related Accession (Same CDS sequence)	NM_001145858.1
Accession Version	NM_001145858.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1812bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	SH3 domain-binding protein 2 isoform c
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK150265.1 and BC085497.1. Transcript Variant: This variant (3) represents use of an alternative promoter and 5' UTR and uses an alternate translation start site, compared to variant 2. The resulting isoform (c) has a shorter and distinct N-terminus, compared to isoform b. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK150265.1, AK155970.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGGGG GATGCAGGTT GCAGTCCCTC CTCATAGCCC CTGAGCTGAA TAGCCTTTCC 
GGATTTAAGG ACACCTGTCC CCAGCAGGTC ACAGGAAGTT GTACCATGTG TTGGGTCAGT 
GCCATGAGCT TCATGGCGGC TGAGGAGATG CAGTGGCCTG TCCCCATGAA GGCCATTGGT 
GCCCAGAATT TGCTGACCAT GCCTGGTGGT GTGGCGAAGG CTGGTTACCT GCATAAGAAG 
GGTGGCACCC AACTGCAGCT GCTCAAATGG CCCCTCCGCT TTGTGATCAT CCACAAGCGC 
TGCATCTACT ACTTCAAGAG CAGCACATCT GCCTCCCCAC AGGGTGCCTT CTCACTGAGT 
GGCTACAACA GAGTGATGCG AGCAGCTGAG GAGACCACGT CCAACAATGT CTTCCCATTC 
AAGATCATTC ACATCAGCAA GAAGCACCGA ACATGGTTCT TCTCAGCGTC CTCTGAGGAT 
GAGCGCAAGA GCTGGATGGC CTTGCTACGC AGGGAAATTG GCCACTTCCA TGAGAAGAAG 
GAACTACCTC TAGACACCAG CGACTCTAGC TCAGACACGG ACAGCTTCTA TGGTGCAGTT 
GAGCGACCTA TAGATATCAG CCTGTCTTCA TATCCCATGG ACAATGAAGA TTACGAACAT 
GAAGATGAGG ACGACTCGTA CCTGGAGCCT GATTCCCCGG GGCCCATGAA GCTTGAAGAT 
GCCCTGACCT ATCCACCAGC CTACCCACCA CCCCCTGTGC CTGTACCTCG GAAGCCAGCC 
TTCTCTGACT TGCCCCGTGC CCACTCGTTC ACCTCTAAAA GTCCAAGTCC CTTACTGCCA 
CCCCCACCTC CTAAGCGAGG CCTTCCAGAC ACTGGCTCTG CCCCTGAGGA TGCTAAGGAC 
GCATTGGGCC TGAGGCGAGT AGAGCCTGGC CTCAGGGTGC CTGCCACCCC TCGGAGGATG 
AGTGACCCCC CAATGAGCAA TGTACCCACC GTGCCCAACC TCCGGAAACA TCCTTGCTTC 
CGGGACAGTG TGAATCCCGG CCTAGAACCC TGGACCCCTG GCCATGGGAC TAGCTCTGTC 
TCCAGCTCTA CCACCATGGC CGTTGCCACC TCTAGGAACT GTGACAAACT CAAGTCATTC 
CACCTGTCTT CCCGAGGGCC ACCCACATCT GAGCCCCCAC CCGTGCCTGC TAATAAGCCC 
AAGTTCCTGA AGATAGCTGA AGAACCTTCC CCGAGGGAGG CAGCCAAGTT TGCACCTGTG 
CCCCCTGTGG CCCCCAGGCC TCCTGTGCAG AAGATGCCCA TGCCTGAGGC TACTGTTCGG 
CCTGCAGTGC TGCCTAGACC AGAGAACACA CCTCTTCCTC ACCTCCAGCG ATCCCCCCCT 
GATGGACAGA GTTTCAGGGG CTTCTCCTTT GAAAAGGCCC GCCAGCCCTC ACAGGCTGAC 
ACTGGCGAGG AGGACTCAGA TGAAGACTAT GAGAAGGTGC CGCTGCCTAA CTCGGTGTTT 
GTCAACACGA CAGAGTCCTG CGAGGTGGAA AGGTTGTTCA AAGCTACGGA CCCCCGTGGA 
GAGCCTCAGG ATGGGCTGTA TTGCATTCGG AACTCCTCTA CCAAGTCAGG GAAGGTTTTG 
GTCGTGTGGG ATGAATCTTC TAACAAAGTG AGGAATTATC GCATCTTTGA AAAGGATTCT 
AAGTTCTACC TGGAAGGTGA GGTTCTGTTT GCCAGTGTGG GCAGCATGGT GGAGCACTAC 
CACACCCATG TGCTGCCCAG CCATCAGAGC CTGTTGCTGC GGCACCCATA CGGCTACGCT 
GGGCCCAGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001139330.1
CDS	91..1902
Translation	MSGGCRLQSLLIAPELNSLSGFKDTCPQQVTGSCTMCWVSAMSFMAAEEMQWPVPMKAIGAQNLLTMPGGVAKAGYLHKKGGTQLQLLKWPLRFVIIHKRCIYYFKSSTSASPQGAFSLSGYNRVMRAAEETTSNNVFPFKIIHISKKHRTWFFSASSEDERKSWMALLRREIGHFHEKKELPLDTSDSSSDTDSFYGAVERPIDISLSSYPMDNEDYEHEDEDDSYLEPDSPGPMKLEDALTYPPAYPPPPVPVPRKPAFSDLPRAHSFTSKSPSPLLPPPPPKRGLPDTGSAPEDAKDALGLRRVEPGLRVPATPRRMSDPPMSNVPTVPNLRKHPCFRDSVNPGLEPWTPGHGTSSVSSSTTMAVATSRNCDKLKSFHLSSRGPPTSEPPPVPANKPKFLKIAEEPSPREAAKFAPVPPVAPRPPVQKMPMPEATVRPAVLPRPENTPLPHLQRSPPDGQSFRGFSFEKARQPSQADTGEEDSDEDYEKVPLPNSVFVNTTESCEVERLFKATDPRGEPQDGLYCIRNSSTKSGKVLVVWDESSNKVRNYRIFEKDSKFYLEGEVLFASVGSMVEHYHTHVLPSHQSLLLRHPYGYAGPR

Target ORF information:

RefSeq Version	NM_001145858.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus SH3-domain binding protein 2 (Sh3bp2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001145858.1

ORF Insert Sequence:

ATGTCTGGGG GATGCAGGTT GCAGTCCCTC CTCATAGCCC CTGAGCTGAA TAGCCTTTCC 
GGATTTAAGG ACACCTGTCC CCAGCAGGTC ACAGGAAGTT GTACCATGTG TTGGGTCAGT 
GCCATGAGCT TCATGGCGGC TGAGGAGATG CAGTGGCCTG TCCCCATGAA GGCCATTGGT 
GCCCAGAATT TGCTGACCAT GCCTGGTGGT GTGGCGAAGG CTGGTTACCT GCATAAGAAG 
GGTGGCACCC AACTGCAGCT GCTCAAATGG CCCCTCCGCT TTGTGATCAT CCACAAGCGC 
TGCATCTACT ACTTCAAGAG CAGCACATCT GCCTCCCCAC AGGGTGCCTT CTCACTGAGT 
GGCTACAACA GAGTGATGCG AGCAGCTGAG GAGACCACGT CCAACAATGT CTTCCCATTC 
AAGATCATTC ACATCAGCAA GAAGCACCGA ACATGGTTCT TCTCAGCGTC CTCTGAGGAT 
GAGCGCAAGA GCTGGATGGC CTTGCTACGC AGGGAAATTG GCCACTTCCA TGAGAAGAAG 
GAACTACCTC TAGACACCAG CGACTCTAGC TCAGACACGG ACAGCTTCTA TGGTGCAGTT 
GAGCGACCTA TAGATATCAG CCTGTCTTCA TATCCCATGG ACAATGAAGA TTACGAACAT 
GAAGATGAGG ACGACTCGTA CCTGGAGCCT GATTCCCCGG GGCCCATGAA GCTTGAAGAT 
GCCCTGACCT ATCCACCAGC CTACCCACCA CCCCCTGTGC CTGTACCTCG GAAGCCAGCC 
TTCTCTGACT TGCCCCGTGC CCACTCGTTC ACCTCTAAAA GTCCAAGTCC CTTACTGCCA 
CCCCCACCTC CTAAGCGAGG CCTTCCAGAC ACTGGCTCTG CCCCTGAGGA TGCTAAGGAC 
GCATTGGGCC TGAGGCGAGT AGAGCCTGGC CTCAGGGTGC CTGCCACCCC TCGGAGGATG 
AGTGACCCCC CAATGAGCAA TGTACCCACC GTGCCCAACC TCCGGAAACA TCCTTGCTTC 
CGGGACAGTG TGAATCCCGG CCTAGAACCC TGGACCCCTG GCCATGGGAC TAGCTCTGTC 
TCCAGCTCTA CCACCATGGC CGTTGCCACC TCTAGGAACT GTGACAAACT CAAGTCATTC 
CACCTGTCTT CCCGAGGGCC ACCCACATCT GAGCCCCCAC CCGTGCCTGC TAATAAGCCC 
AAGTTCCTGA AGATAGCTGA AGAACCTTCC CCGAGGGAGG CAGCCAAGTT TGCACCTGTG 
CCCCCTGTGG CCCCCAGGCC TCCTGTGCAG AAGATGCCCA TGCCTGAGGC TACTGTTCGG 
CCTGCAGTGC TGCCTAGACC AGAGAACACA CCTCTTCCTC ACCTCCAGCG ATCCCCCCCT 
GATGGACAGA GTTTCAGGGG CTTCTCCTTT GAAAAGGCCC GCCAGCCCTC ACAGGCTGAC 
ACTGGCGAGG AGGACTCAGA TGAAGACTAT GAGAAGGTGC CGCTGCCTAA CTCGGTGTTT 
GTCAACACGA CAGAGTCCTG CGAGGTGGAA AGGTTGTTCA AAGCTACGGA CCCCCGTGGA 
GAGCCTCAGG ATGGGCTGTA TTGCATTCGG AACTCCTCTA CCAAGTCAGG GAAGGTTTTG 
GTCGTGTGGG ATGAATCTTC TAACAAAGTG AGGAATTATC GCATCTTTGA AAAGGATTCT 
AAGTTCTACC TGGAAGGTGA GGTTCTGTTT GCCAGTGTGG GCAGCATGGT GGAGCACTAC 
CACACCCATG TGCTGCCCAG CCATCAGAGC CTGTTGCTGC GGCACCCATA CGGCTACGCT 
GGGCCCAGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sh3bp2 ( NM_001145859.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001139331.1 Mus musculus SH3-domain binding protein 2 (Sh3bp2), transcript variant 2, mRNA.

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CloneID	OMu06990
Clone ID Related Accession (Same CDS sequence)	NM_001145859.1
Accession Version	NM_001145859.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1848bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	SH3 domain-binding protein 2 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK143548.1 and BC085497.1. Transcript Variant: This variant (2) represents the longest transcript and encodes the longest isoform (b). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC085497.1, AK143548.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGGCT CAGGGTCGCG GCCGCGGAGC TGGGGCTGGC GAGAGACAGG CTCCAGGGAC 
GAGGCTGCGG CCGGTGGGCC CACCTCGGGA CCCTGTCGCT GCTCACAGGG AAGGCGGGCG 
CTGGCCGCCC CCCGGAAGCC GGCTGTGCCC GCCGCGTGGA CACCCTTCAT GGCGGCTGAG 
GAGATGCAGT GGCCTGTCCC CATGAAGGCC ATTGGTGCCC AGAATTTGCT GACCATGCCT 
GGTGGTGTGG CGAAGGCTGG TTACCTGCAT AAGAAGGGTG GCACCCAACT GCAGCTGCTC 
AAATGGCCCC TCCGCTTTGT GATCATCCAC AAGCGCTGCA TCTACTACTT CAAGAGCAGC 
ACATCTGCCT CCCCACAGGG TGCCTTCTCA CTGAGTGGCT ACAACAGAGT GATGCGAGCA 
GCTGAGGAGA CCACGTCCAA CAATGTCTTC CCATTCAAGA TCATTCACAT CAGCAAGAAG 
CACCGAACAT GGTTCTTCTC AGCGTCCTCT GAGGATGAGC GCAAGAGCTG GATGGCCTTG 
CTACGCAGGG AAATTGGCCA CTTCCATGAG AAGAAGGAAC TACCTCTAGA CACCAGCGAC 
TCTAGCTCAG ACACGGACAG CTTCTATGGT GCAGTTGAGC GACCTATAGA TATCAGCCTG 
TCTTCATATC CCATGGACAA TGAAGATTAC GAACATGAAG ATGAGGACGA CTCGTACCTG 
GAGCCTGATT CCCCGGGGCC CATGAAGCTT GAAGATGCCC TGACCTATCC ACCAGCCTAC 
CCACCACCCC CTGTGCCTGT ACCTCGGAAG CCAGCCTTCT CTGACTTGCC CCGTGCCCAC 
TCGTTCACCT CTAAAAGTCC AAGTCCCTTA CTGCCACCCC CACCTCCTAA GCGAGGCCTT 
CCAGACACTG GCTCTGCCCC TGAGGATGCT AAGGACGCAT TGGGCCTGAG GCGAGTAGAG 
CCTGGCCTCA GGGTGCCTGC CACCCCTCGG AGGATGAGTG ACCCCCCAAT GAGCAATGTA 
CCCACCGTGC CCAACCTCCG GAAACATCCT TGCTTCCGGG ACAGTGTGAA TCCCGGCCTA 
GAACCCTGGA CCCCTGGCCA TGGGACTAGC TCTGTCTCCA GCTCTACCAC CATGGCCGTT 
GCCACCTCTA GGAACTGTGA CAAACTCAAG TCATTCCACC TGTCTTCCCG AGGGCCACCC 
ACATCTGAGC CCCCACCCGT GCCTGCTAAT AAGCCCAAGT TCCTGAAGAT AGCTGAAGAA 
CCTTCCCCGA GGGAGGCAGC CAAGTTTGCA CCTGTGCCCC CTGTGGCCCC CAGGCCTCCT 
GTGCAGAAGA TGCCCATGCC TGAGGCTACT GTTCGGCCTG CAGTGCTGCC TAGACCAGAG 
AACACACCTC TTCCTCACCT CCAGCGATCC CCCCCTGATG GACAGAGTTT CAGGGGCTTC 
TCCTTTGAAA AGGCCCGCCA GCCCTCACAG GCTGACACTG GCGAGGAGGA CTCAGATGAA 
GACTATGAGA AGGTGCCGCT GCCTAACTCG GTGTTTGTCA ACACGACAGA GTCCTGCGAG 
GTGGAAAGGT TGTTCAAAGC TACGGACCCC CGTGGAGAGC CTCAGGATGG GCTGTATTGC 
ATTCGGAACT CCTCTACCAA GTCAGGGAAG GTTTTGGTCG TGTGGGATGA ATCTTCTAAC 
AAAGTGAGGA ATTATCGCAT CTTTGAAAAG GATTCTAAGT TCTACCTGGA AGGTGAGGTT 
CTGTTTGCCA GTGTGGGCAG CATGGTGGAG CACTACCACA CCCATGTGCT GCCCAGCCAT 
CAGAGCCTGT TGCTGCGGCA CCCATACGGC TACGCTGGGC CCAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001139331.1
CDS	58..1905
Translation	MAGSGSRPRSWGWRETGSRDEAAAGGPTSGPCRCSQGRRALAAPRKPAVPAAWTPFMAAEEMQWPVPMKAIGAQNLLTMPGGVAKAGYLHKKGGTQLQLLKWPLRFVIIHKRCIYYFKSSTSASPQGAFSLSGYNRVMRAAEETTSNNVFPFKIIHISKKHRTWFFSASSEDERKSWMALLRREIGHFHEKKELPLDTSDSSSDTDSFYGAVERPIDISLSSYPMDNEDYEHEDEDDSYLEPDSPGPMKLEDALTYPPAYPPPPVPVPRKPAFSDLPRAHSFTSKSPSPLLPPPPPKRGLPDTGSAPEDAKDALGLRRVEPGLRVPATPRRMSDPPMSNVPTVPNLRKHPCFRDSVNPGLEPWTPGHGTSSVSSSTTMAVATSRNCDKLKSFHLSSRGPPTSEPPPVPANKPKFLKIAEEPSPREAAKFAPVPPVAPRPPVQKMPMPEATVRPAVLPRPENTPLPHLQRSPPDGQSFRGFSFEKARQPSQADTGEEDSDEDYEKVPLPNSVFVNTTESCEVERLFKATDPRGEPQDGLYCIRNSSTKSGKVLVVWDESSNKVRNYRIFEKDSKFYLEGEVLFASVGSMVEHYHTHVLPSHQSLLLRHPYGYAGPR

Target ORF information:

RefSeq Version	NM_001145859.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus SH3-domain binding protein 2 (Sh3bp2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001145859.1

ORF Insert Sequence:

ATGGCGGGCT CAGGGTCGCG GCCGCGGAGC TGGGGCTGGC GAGAGACAGG CTCCAGGGAC 
GAGGCTGCGG CCGGTGGGCC CACCTCGGGA CCCTGTCGCT GCTCACAGGG AAGGCGGGCG 
CTGGCCGCCC CCCGGAAGCC GGCTGTGCCC GCCGCGTGGA CACCCTTCAT GGCGGCTGAG 
GAGATGCAGT GGCCTGTCCC CATGAAGGCC ATTGGTGCCC AGAATTTGCT GACCATGCCT 
GGTGGTGTGG CGAAGGCTGG TTACCTGCAT AAGAAGGGTG GCACCCAACT GCAGCTGCTC 
AAATGGCCCC TCCGCTTTGT GATCATCCAC AAGCGCTGCA TCTACTACTT CAAGAGCAGC 
ACATCTGCCT CCCCACAGGG TGCCTTCTCA CTGAGTGGCT ACAACAGAGT GATGCGAGCA 
GCTGAGGAGA CCACGTCCAA CAATGTCTTC CCATTCAAGA TCATTCACAT CAGCAAGAAG 
CACCGAACAT GGTTCTTCTC AGCGTCCTCT GAGGATGAGC GCAAGAGCTG GATGGCCTTG 
CTACGCAGGG AAATTGGCCA CTTCCATGAG AAGAAGGAAC TACCTCTAGA CACCAGCGAC 
TCTAGCTCAG ACACGGACAG CTTCTATGGT GCAGTTGAGC GACCTATAGA TATCAGCCTG 
TCTTCATATC CCATGGACAA TGAAGATTAC GAACATGAAG ATGAGGACGA CTCGTACCTG 
GAGCCTGATT CCCCGGGGCC CATGAAGCTT GAAGATGCCC TGACCTATCC ACCAGCCTAC 
CCACCACCCC CTGTGCCTGT ACCTCGGAAG CCAGCCTTCT CTGACTTGCC CCGTGCCCAC 
TCGTTCACCT CTAAAAGTCC AAGTCCCTTA CTGCCACCCC CACCTCCTAA GCGAGGCCTT 
CCAGACACTG GCTCTGCCCC TGAGGATGCT AAGGACGCAT TGGGCCTGAG GCGAGTAGAG 
CCTGGCCTCA GGGTGCCTGC CACCCCTCGG AGGATGAGTG ACCCCCCAAT GAGCAATGTA 
CCCACCGTGC CCAACCTCCG GAAACATCCT TGCTTCCGGG ACAGTGTGAA TCCCGGCCTA 
GAACCCTGGA CCCCTGGCCA TGGGACTAGC TCTGTCTCCA GCTCTACCAC CATGGCCGTT 
GCCACCTCTA GGAACTGTGA CAAACTCAAG TCATTCCACC TGTCTTCCCG AGGGCCACCC 
ACATCTGAGC CCCCACCCGT GCCTGCTAAT AAGCCCAAGT TCCTGAAGAT AGCTGAAGAA 
CCTTCCCCGA GGGAGGCAGC CAAGTTTGCA CCTGTGCCCC CTGTGGCCCC CAGGCCTCCT 
GTGCAGAAGA TGCCCATGCC TGAGGCTACT GTTCGGCCTG CAGTGCTGCC TAGACCAGAG 
AACACACCTC TTCCTCACCT CCAGCGATCC CCCCCTGATG GACAGAGTTT CAGGGGCTTC 
TCCTTTGAAA AGGCCCGCCA GCCCTCACAG GCTGACACTG GCGAGGAGGA CTCAGATGAA 
GACTATGAGA AGGTGCCGCT GCCTAACTCG GTGTTTGTCA ACACGACAGA GTCCTGCGAG 
GTGGAAAGGT TGTTCAAAGC TACGGACCCC CGTGGAGAGC CTCAGGATGG GCTGTATTGC 
ATTCGGAACT CCTCTACCAA GTCAGGGAAG GTTTTGGTCG TGTGGGATGA ATCTTCTAAC 
AAAGTGAGGA ATTATCGCAT CTTTGAAAAG GATTCTAAGT TCTACCTGGA AGGTGAGGTT 
CTGTTTGCCA GTGTGGGCAG CATGGTGGAG CACTACCACA CCCATGTGCT GCCCAGCCAT 
CAGAGCCTGT TGCTGCGGCA CCCATACGGC TACGCTGGGC CCAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sh3bp2 ( XM_006503954.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006504017.1 Mus musculus SH3-domain binding protein 2 (Sh3bp2), transcript variant X1, mRNA.

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CloneID	OMu09104
Clone ID Related Accession (Same CDS sequence)	XM_006503955.1 , NM_011893.3 , XM_006503954.2 , XM_006503954.1
Accession Version	XM_006503954.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1680bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	SH3 domain-binding protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006503954.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTG AGGAGATGCA GTGGCCTGTC CCCATGAAGG CCATTGGTGC CCAGAATTTG 
CTGACCATGC CTGGTGGTGT GGCGAAGGCT GGTTACCTGC ATAAGAAGGG TGGCACCCAA 
CTGCAGCTGC TCAAATGGCC CCTCCGCTTT GTGATCATCC ACAAGCGCTG CATCTACTAC 
TTCAAGAGCA GCACATCTGC CTCCCCACAG GGTGCCTTCT CACTGAGTGG CTACAACAGA 
GTGATGCGAG CAGCTGAGGA GACCACGTCC AACAATGTCT TCCCATTCAA GATCATTCAC 
ATCAGCAAGA AGCACCGAAC ATGGTTCTTC TCAGCGTCCT CTGAGGATGA GCGCAAGAGC 
TGGATGGCCT TGCTACGCAG GGAAATTGGC CACTTCCATG AGAAGAAGGA ACTACCTCTA 
GACACCAGCG ACTCTAGCTC AGACACGGAC AGCTTCTATG GTGCAGTTGA GCGACCTATA 
GATATCAGCC TGTCTTCATA TCCCATGGAC AATGAAGATT ACGAACATGA AGATGAGGAC 
GACTCGTACC TGGAGCCTGA TTCCCCGGGG CCCATGAAGC TTGAAGATGC CCTGACCTAT 
CCACCAGCCT ACCCACCACC CCCTGTGCCT GTACCTCGGA AGCCAGCCTT CTCTGACTTG 
CCCCGTGCCC ACTCGTTCAC CTCTAAAAGT CCAAGTCCCT TACTGCCACC CCCACCTCCT 
AAGCGAGGCC TTCCAGACAC TGGCTCTGCC CCTGAGGATG CTAAGGACGC ATTGGGCCTG 
AGGCGAGTAG AGCCTGGCCT CAGGGTGCCT GCCACCCCTC GGAGGATGAG TGACCCCCCA 
ATGAGCAATG TACCCACCGT GCCCAACCTC CGGAAACATC CTTGCTTCCG GGACAGTGTG 
AATCCCGGCC TAGAACCCTG GACCCCTGGC CATGGGACTA GCTCTGTCTC CAGCTCTACC 
ACCATGGCCG TTGCCACCTC TAGGAACTGT GACAAACTCA AGTCATTCCA CCTGTCTTCC 
CGAGGGCCAC CCACATCTGA GCCCCCACCC GTGCCTGCTA ATAAGCCCAA GTTCCTGAAG 
ATAGCTGAAG AACCTTCCCC GAGGGAGGCA GCCAAGTTTG CACCTGTGCC CCCTGTGGCC 
CCCAGGCCTC CTGTGCAGAA GATGCCCATG CCTGAGGCTA CTGTTCGGCC TGCAGTGCTG 
CCTAGACCAG AGAACACACC TCTTCCTCAC CTCCAGCGAT CCCCCCCTGA TGGACAGAGT 
TTCAGGGGCT TCTCCTTTGA AAAGGCCCGC CAGCCCTCAC AGGCTGACAC TGGCGAGGAG 
GACTCAGATG AAGACTATGA GAAGGTGCCG CTGCCTAACT CGGTGTTTGT CAACACGACA 
GAGTCCTGCG AGGTGGAAAG GTTGTTCAAA GCTACGGACC CCCGTGGAGA GCCTCAGGAT 
GGGCTGTATT GCATTCGGAA CTCCTCTACC AAGTCAGGGA AGGTTTTGGT CGTGTGGGAT 
GAATCTTCTA ACAAAGTGAG GAATTATCGC ATCTTTGAAA AGGATTCTAA GTTCTACCTG 
GAAGGTGAGG TTCTGTTTGC CAGTGTGGGC AGCATGGTGG AGCACTACCA CACCCATGTG 
CTGCCCAGCC ATCAGAGCCT GTTGCTGCGG CACCCATACG GCTACGCTGG GCCCAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006504017.1
CDS	29..1708
Translation	MAAEEMQWPVPMKAIGAQNLLTMPGGVAKAGYLHKKGGTQLQLLKWPLRFVIIHKRCIYYFKSSTSASPQGAFSLSGYNRVMRAAEETTSNNVFPFKIIHISKKHRTWFFSASSEDERKSWMALLRREIGHFHEKKELPLDTSDSSSDTDSFYGAVERPIDISLSSYPMDNEDYEHEDEDDSYLEPDSPGPMKLEDALTYPPAYPPPPVPVPRKPAFSDLPRAHSFTSKSPSPLLPPPPPKRGLPDTGSAPEDAKDALGLRRVEPGLRVPATPRRMSDPPMSNVPTVPNLRKHPCFRDSVNPGLEPWTPGHGTSSVSSSTTMAVATSRNCDKLKSFHLSSRGPPTSEPPPVPANKPKFLKIAEEPSPREAAKFAPVPPVAPRPPVQKMPMPEATVRPAVLPRPENTPLPHLQRSPPDGQSFRGFSFEKARQPSQADTGEEDSDEDYEKVPLPNSVFVNTTESCEVERLFKATDPRGEPQDGLYCIRNSSTKSGKVLVVWDESSNKVRNYRIFEKDSKFYLEGEVLFASVGSMVEHYHTHVLPSHQSLLLRHPYGYAGPR

Target ORF information:

RefSeq Version	XM_006503954.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus SH3-domain binding protein 2 (Sh3bp2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006503954.2

ORF Insert Sequence:

ATGGCGGCTG AGGAGATGCA GTGGCCTGTC CCCATGAAGG CCATTGGTGC CCAGAATTTG 
CTGACCATGC CTGGTGGTGT GGCGAAGGCT GGTTACCTGC ATAAGAAGGG TGGCACCCAA 
CTGCAGCTGC TCAAATGGCC CCTCCGCTTT GTGATCATCC ACAAGCGCTG CATCTACTAC 
TTCAAGAGCA GCACATCTGC CTCCCCACAG GGTGCCTTCT CACTGAGTGG CTACAACAGA 
GTGATGCGAG CAGCTGAGGA GACCACGTCC AACAATGTCT TCCCATTCAA GATCATTCAC 
ATCAGCAAGA AGCACCGAAC ATGGTTCTTC TCAGCGTCCT CTGAGGATGA GCGCAAGAGC 
TGGATGGCCT TGCTACGCAG GGAAATTGGC CACTTCCATG AGAAGAAGGA ACTACCTCTA 
GACACCAGCG ACTCTAGCTC AGACACGGAC AGCTTCTATG GTGCAGTTGA GCGACCTATA 
GATATCAGCC TGTCTTCATA TCCCATGGAC AATGAAGATT ACGAACATGA AGATGAGGAC 
GACTCGTACC TGGAGCCTGA TTCCCCGGGG CCCATGAAGC TTGAAGATGC CCTGACCTAT 
CCACCAGCCT ACCCACCACC CCCTGTGCCT GTACCTCGGA AGCCAGCCTT CTCTGACTTG 
CCCCGTGCCC ACTCGTTCAC CTCTAAAAGT CCAAGTCCCT TACTGCCACC CCCACCTCCT 
AAGCGAGGCC TTCCAGACAC TGGCTCTGCC CCTGAGGATG CTAAGGACGC ATTGGGCCTG 
AGGCGAGTAG AGCCTGGCCT CAGGGTGCCT GCCACCCCTC GGAGGATGAG TGACCCCCCA 
ATGAGCAATG TACCCACCGT GCCCAACCTC CGGAAACATC CTTGCTTCCG GGACAGTGTG 
AATCCCGGCC TAGAACCCTG GACCCCTGGC CATGGGACTA GCTCTGTCTC CAGCTCTACC 
ACCATGGCCG TTGCCACCTC TAGGAACTGT GACAAACTCA AGTCATTCCA CCTGTCTTCC 
CGAGGGCCAC CCACATCTGA GCCCCCACCC GTGCCTGCTA ATAAGCCCAA GTTCCTGAAG 
ATAGCTGAAG AACCTTCCCC GAGGGAGGCA GCCAAGTTTG CACCTGTGCC CCCTGTGGCC 
CCCAGGCCTC CTGTGCAGAA GATGCCCATG CCTGAGGCTA CTGTTCGGCC TGCAGTGCTG 
CCTAGACCAG AGAACACACC TCTTCCTCAC CTCCAGCGAT CCCCCCCTGA TGGACAGAGT 
TTCAGGGGCT TCTCCTTTGA AAAGGCCCGC CAGCCCTCAC AGGCTGACAC TGGCGAGGAG 
GACTCAGATG AAGACTATGA GAAGGTGCCG CTGCCTAACT CGGTGTTTGT CAACACGACA 
GAGTCCTGCG AGGTGGAAAG GTTGTTCAAA GCTACGGACC CCCGTGGAGA GCCTCAGGAT 
GGGCTGTATT GCATTCGGAA CTCCTCTACC AAGTCAGGGA AGGTTTTGGT CGTGTGGGAT 
GAATCTTCTA ACAAAGTGAG GAATTATCGC ATCTTTGAAA AGGATTCTAA GTTCTACCTG 
GAAGGTGAGG TTCTGTTTGC CAGTGTGGGC AGCATGGTGG AGCACTACCA CACCCATGTG 
CTGCCCAGCC ATCAGAGCCT GTTGCTGCGG CACCCATACG GCTACGCTGG GCCCAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RANKL coordinates multiple osteoclastogenic pathways by regulating expression of ubiquitin ligase RNF146.
The Journal of clinical investigation127(4)1303-1315(2017 Apr)
Matsumoto Y,Larose J,Kent OA,Lim M,Changoor A,Zhang L,Storozhuk Y,Mao X,Grynpas MD,Cong F,Rottapel R

Reciprocal stabilization of ABL and TAZ regulates osteoblastogenesis through transcription factor RUNX2.
The Journal of clinical investigation126(12)4482-4496(2016 Dec)
Matsumoto Y,La Rose J,Kent OA,Wagner MJ,Narimatsu M,Levy AD,Omar MH,Tong J,Krieger JR,Riggs E,Storozhuk Y,Pasquale J,Ventura M,Yeganeh B,Post M,Moran MF,Grynpas MD,Wrana JL,Superti-Furga G,Koleske AJ,Pendergast AM,Rottapel R

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

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Sh3bp2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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