Mrps12 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mrps12
Entrez Gene ID	24030
Full Name	mitochondrial ribosomal protein S12
Synonyms	AI327385,Rpms12,rps12
General protein information	Preferred Names mitochondrial ribosomal protein S12 Names 28S ribosomal protein S12, mitochondrial MRP-S12 MT-RPS12 S12mt mitoribosomal protein s12 ribosomal protein, mitochondrial, S12
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 B1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001360254.1	NP_001347183.1	28S ribosomal protein S12, mitochondrial isoform b
NM_011885.5	NP_036015.1	28S ribosomal protein S12, mitochondrial isoform a precursor
NM_001360252.1	NP_001347181.1	28S ribosomal protein S12, mitochondrial isoform a precursor
NM_011885.4	NP_036015.1	28S ribosomal protein S12, mitochondrial precursor
XM_006539908.2	XP_006539971.1	28S ribosomal protein S12, mitochondrial isoform X1
XM_006539908.1	XP_006539971.1	28S ribosomal protein S12, mitochondrial isoform X1
NM_001360250.1	NP_001347179.1	28S ribosomal protein S12, mitochondrial isoform a precursor

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	MRPS12	XP_001233807.1
Drosophila melanogaster (fruit fly)	tko	NP_001245490.1
Caenorhabditis elegans (roundworm)	mrps-12	NP_508023.1
Xenopus tropicalis (tropical clawed frog)	mrps12	NP_001037988.1
Pan troglodytes (chimpanzee)	MRPS12	XP_001135068.1
Bos taurus (cattle)	MRPS12	NP_001070569.1
Rattus norvegicus (Norway rat)	Mrps12	NP_001099709.1
Danio rerio (zebrafish)	LOC100537619	XP_003200900.1
Saccharomyces cerevisiae (baker's yeast)	MRPS12	NP_014434.1
Kluyveromyces lactis	KLLA0F07007g	XP_455398.1
Oryza sativa (rice)	Os04g0234750	NP_001173808.1
Magnaporthe oryzae (rice blast fungus)	MGG_08691	XP_003719290.1
Neurospora crassa	NCU06542	XP_962249.2
Homo sapiens (human)	MRPS12	NP_066930.1
Canis lupus familiaris (dog)	MRPS12	XP_541631.3
Mus musculus (house mouse)	Mrps12	NP_036015.1
Danio rerio (zebrafish)	si:dkey-17e16.10	XP_005161333.1
Eremothecium gossypii	AGOS_AGL222W	NP_986445.1
Oryza sativa (rice)	rps12	NP_039359.1
	MRPS12	XP_001086380.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP013102	XP_003435965.1
Schizosaccharomyces pombe (fission yeast)	SPAC4F8.06	NP_593866.2

Gene Ontology
Bibliography

Related articles in PubMed

Decreased expression levels of Ifi genes is associated to the increased resistance to spontaneous arthritis disease in mice deficiency of IL-1RA.
Liu X, Jiao Y, Cao Y, Deng N, Ma Y, Hasty KA, Kang A, Chen H, Stuart JM, Gu W
BMC immunology17(1)25(2016 08)

NF-Y influences directionality of transcription from the bidirectional Mrps12/Sarsm promoter in both mouse and human cells.
Zanotto E, H?kkinen A, Teku G, Shen B, Ribeiro AS, Jacobs HT
Biochimica et biophysica acta1789(5)432-42(2009 May)

The bidirectional promoter of two genes for the mitochondrial translational apparatus in mouse is regulated by an array of CCAAT boxes interacting with the transcription factor NF-Y.
Zanotto E, Shah ZH, Jacobs HT
Nucleic acids research35(2)664-77(2007)

Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria.
Mootha VK, Bunkenborg J, Olsen JV, Hjerrild M, Wisniewski JR, Stahl E, Bolouri MS, Ray HN, Sihag S, Kamal M, Patterson N, Lander ES, Mann M
Cell115(5)629-40(2003 Nov)

Proteomic analysis of the mammalian mitochondrial ribosome. Identification of protein components in the 28 S small subunit.
Suzuki T, Terasaki M, Takemoto-Hori C, Hanada T, Ueda T, Wada A, Watanabe K
The Journal of biological chemistry276(35)33181-95(2001 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

NF-Y type CCAAT boxes are found preferentially in bidirectional Mrps12/Sarsm promoters, but many such promoters lack them and must be regulated in another way.
Title: NF-Y influences directionality of transcription from the bidirectional Mrps12/Sarsm promoter in both mouse and human cells.

Identification of an array of 4 CCAAT box elements required for efficient transcription of the Mrps12 gene.
Title: The bidirectional promoter of two genes for the mitochondrial translational apparatus in mouse is regulated by an array of CCAAT boxes interacting with the transcription factor NF-Y.

The following Mrps12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mrps12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu13710	NM_001360252.1 Latest version!	Mus musculus mitochondrial ribosomal protein S12 (Mrps12), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu13710	NM_001360250.1 Latest version!	Mus musculus mitochondrial ribosomal protein S12 (Mrps12), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu13710	NM_011885.5 Latest version!	Mus musculus mitochondrial ribosomal protein S12 (Mrps12), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu84139	NM_001360254.1 Latest version!	Mus musculus mitochondrial ribosomal protein S12 (Mrps12), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OMu13710	XM_006539908.2 Latest version!	Mus musculus mitochondrial ribosomal protein S12 (Mrps12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
View Old Accession Versions >>
OMu13710	NM_011885.4	Mus musculus mitochondrial ribosomal protein S12 (Mrps12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu13710	XM_006539908.1	Mus musculus mitochondrial ribosomal protein S12 (Mrps12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mrps12 ( NM_011885.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_036015.1 Mus musculus mitochondrial ribosomal protein S12 (Mrps12), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu13710
Clone ID Related Accession (Same CDS sequence)	NM_011885.5 , NM_001360252.1 , NM_011885.4 , XM_006539908.2 , XM_006539908.1 , NM_001360250.1
Accession Version	NM_011885.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-06
Organism	Mus musculus(house mouse)
Product	28S ribosomal protein S12, mitochondrial isoform a precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC171210.1 and Y11682.1. On Jan 30, 2018 this sequence version replaced NM_011885.4. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2, and 3 all encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC027522.1, BU604005.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCCTGGC CCGGCCTTCT TTATGGCCTC ACCACGTCCC TCAGTCGTGG CCTAGCCCTC 
GCTCCCCAGC TCTGGGCCGC CCGTTCCATG GCCACCCTGA ACCAGATGCA CCGCTTAGGC 
CCCCGGAAGG AACCCCCTAA GCGTCTGGGC CCCACAGAGG GCCGGCCCCA GCTGAAGGGT 
GTGGTGTTGC GCACATTCAT CCGAAAGCCG AAGAAGCCCA ACTCCGCCAA CCGCAAGTGC 
TGCCGAGTCC GCCTCAGCAC AGGCAAAGAG GCTGTCTGCT TCATCCCCGG GGAGGGCCAC 
ACCCTGCAGG AGCATCACGT GGTCCTCGTG GAGGGTGGTC GCACCCAGGA CCTGCCTGGG 
GTCAAACTCA AAGTTGTGAG GGGCAAGTAT GACTGCGGCC ACGTGCAAAA GAAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036015.1
CDS	77..496
Translation	MSWPGLLYGLTTSLSRGLALAPQLWAARSMATLNQMHRLGPRKEPPKRLGPTEGRPQLKGVVLRTFIRKPKKPNSANRKCCRVRLSTGKEAVCFIPGEGHTLQEHHVVLVEGGRTQDLPGVKLKVVRGKYDCGHVQKKK

Target ORF information:

RefSeq Version	NM_011885.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus mitochondrial ribosomal protein S12 (Mrps12), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011885.5

ORF Insert Sequence:

ATGTCCTGGC CCGGCCTTCT TTATGGCCTC ACCACGTCCC TCAGTCGTGG CCTAGCCCTC 
GCTCCCCAGC TCTGGGCCGC CCGTTCCATG GCCACCCTGA ACCAGATGCA CCGCTTAGGC 
CCCCGGAAGG AACCCCCTAA GCGTCTGGGC CCCACAGAGG GCCGGCCCCA GCTGAAGGGT 
GTGGTGTTGC GCACATTCAT CCGAAAGCCG AAGAAGCCCA ACTCCGCCAA CCGCAAGTGC 
TGCCGAGTCC GCCTCAGCAC AGGCAAAGAG GCTGTCTGCT TCATCCCCGG GGAGGGCCAC 
ACCCTGCAGG AGCATCACGT GGTCCTCGTG GAGGGTGGTC GCACCCAGGA CCTGCCTGGG 
GTCAAACTCA AAGTTGTGAG GGGCAAGTAT GACTGCGGCC ACGTGCAAAA GAAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mrps12 ( NM_001360252.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001347181.1 Mus musculus mitochondrial ribosomal protein S12 (Mrps12), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu13710
Clone ID Related Accession (Same CDS sequence)	NM_011885.5 , NM_001360252.1 , NM_011885.4 , XM_006539908.2 , XM_006539908.1 , NM_001360250.1
Accession Version	NM_001360252.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Mus musculus(house mouse)
Product	28S ribosomal protein S12, mitochondrial isoform a precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC171210.1. On Jan 30, 2018 this sequence version replaced XM_006539908.2. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2, and 3 all encode the same isoform (a). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AV493585.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCCTGGC CCGGCCTTCT TTATGGCCTC ACCACGTCCC TCAGTCGTGG CCTAGCCCTC 
GCTCCCCAGC TCTGGGCCGC CCGTTCCATG GCCACCCTGA ACCAGATGCA CCGCTTAGGC 
CCCCGGAAGG AACCCCCTAA GCGTCTGGGC CCCACAGAGG GCCGGCCCCA GCTGAAGGGT 
GTGGTGTTGC GCACATTCAT CCGAAAGCCG AAGAAGCCCA ACTCCGCCAA CCGCAAGTGC 
TGCCGAGTCC GCCTCAGCAC AGGCAAAGAG GCTGTCTGCT TCATCCCCGG GGAGGGCCAC 
ACCCTGCAGG AGCATCACGT GGTCCTCGTG GAGGGTGGTC GCACCCAGGA CCTGCCTGGG 
GTCAAACTCA AAGTTGTGAG GGGCAAGTAT GACTGCGGCC ACGTGCAAAA GAAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001347181.1
CDS	37..456
Translation	MSWPGLLYGLTTSLSRGLALAPQLWAARSMATLNQMHRLGPRKEPPKRLGPTEGRPQLKGVVLRTFIRKPKKPNSANRKCCRVRLSTGKEAVCFIPGEGHTLQEHHVVLVEGGRTQDLPGVKLKVVRGKYDCGHVQKKK

Target ORF information:

RefSeq Version	NM_001360252.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mitochondrial ribosomal protein S12 (Mrps12), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001360252.1

ORF Insert Sequence:

ATGTCCTGGC CCGGCCTTCT TTATGGCCTC ACCACGTCCC TCAGTCGTGG CCTAGCCCTC 
GCTCCCCAGC TCTGGGCCGC CCGTTCCATG GCCACCCTGA ACCAGATGCA CCGCTTAGGC 
CCCCGGAAGG AACCCCCTAA GCGTCTGGGC CCCACAGAGG GCCGGCCCCA GCTGAAGGGT 
GTGGTGTTGC GCACATTCAT CCGAAAGCCG AAGAAGCCCA ACTCCGCCAA CCGCAAGTGC 
TGCCGAGTCC GCCTCAGCAC AGGCAAAGAG GCTGTCTGCT TCATCCCCGG GGAGGGCCAC 
ACCCTGCAGG AGCATCACGT GGTCCTCGTG GAGGGTGGTC GCACCCAGGA CCTGCCTGGG 
GTCAAACTCA AAGTTGTGAG GGGCAAGTAT GACTGCGGCC ACGTGCAAAA GAAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mrps12 ( NM_011885.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_036015.1 Mus musculus mitochondrial ribosomal protein S12 (Mrps12), mRNA.

Price & Availability↑

CloneID	OMu13710
Clone ID Related Accession (Same CDS sequence)	NM_011885.5 , NM_001360252.1 , NM_011885.4 , XM_006539908.2 , XM_006539908.1 , NM_001360250.1
Accession Version	NM_011885.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-27
Organism	Mus musculus(house mouse)
Product	28S ribosomal protein S12, mitochondrial precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC171210.1. On Aug 22, 2007 this sequence version replaced NM_011885.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC027522.1, BF531552.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGTCCTGGC CCGGCCTTCT TTATGGCCTC ACCACGTCCC TCAGTCGTGG CCTAGCCCTC 
GCTCCCCAGC TCTGGGCCGC CCGTTCCATG GCCACCCTGA ACCAGATGCA CCGCTTAGGC 
CCCCGGAAGG AACCCCCTAA GCGTCTGGGC CCCACAGAGG GCCGGCCCCA GCTGAAGGGT 
GTGGTGTTGC GCACATTCAT CCGAAAGCCG AAGAAGCCCA ACTCCGCCAA CCGCAAGTGC 
TGCCGAGTCC GCCTCAGCAC AGGCAAAGAG GCTGTCTGCT TCATCCCCGG GGAGGGCCAC 
ACCCTGCAGG AGCATCACGT GGTCCTCGTG GAGGGTGGTC GCACCCAGGA CCTGCCTGGG 
GTCAAACTCA AAGTTGTGAG GGGCAAGTAT GACTGCGGCC ACGTGCAAAA GAAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036015.1
CDS	37..456
Translation	MSWPGLLYGLTTSLSRGLALAPQLWAARSMATLNQMHRLGPRKEPPKRLGPTEGRPQLKGVVLRTFIRKPKKPNSANRKCCRVRLSTGKEAVCFIPGEGHTLQEHHVVLVEGGRTQDLPGVKLKVVRGKYDCGHVQKKK

Target ORF information:

RefSeq Version	NM_011885.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus mitochondrial ribosomal protein S12 (Mrps12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011885.4

ORF Insert Sequence:

ATGTCCTGGC CCGGCCTTCT TTATGGCCTC ACCACGTCCC TCAGTCGTGG CCTAGCCCTC 
GCTCCCCAGC TCTGGGCCGC CCGTTCCATG GCCACCCTGA ACCAGATGCA CCGCTTAGGC 
CCCCGGAAGG AACCCCCTAA GCGTCTGGGC CCCACAGAGG GCCGGCCCCA GCTGAAGGGT 
GTGGTGTTGC GCACATTCAT CCGAAAGCCG AAGAAGCCCA ACTCCGCCAA CCGCAAGTGC 
TGCCGAGTCC GCCTCAGCAC AGGCAAAGAG GCTGTCTGCT TCATCCCCGG GGAGGGCCAC 
ACCCTGCAGG AGCATCACGT GGTCCTCGTG GAGGGTGGTC GCACCCAGGA CCTGCCTGGG 
GTCAAACTCA AAGTTGTGAG GGGCAAGTAT GACTGCGGCC ACGTGCAAAA GAAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mrps12 ( NM_001360250.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001347179.1 Mus musculus mitochondrial ribosomal protein S12 (Mrps12), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu13710
Clone ID Related Accession (Same CDS sequence)	NM_011885.5 , NM_001360252.1 , NM_011885.4 , XM_006539908.2 , XM_006539908.1 , NM_001360250.1
Accession Version	NM_001360250.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Mus musculus(house mouse)
Product	28S ribosomal protein S12, mitochondrial isoform a precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC171210.1 and CA323119.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Variants 1, 2, and 3 all encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: CA323119.1, CA467888.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCCTGGC CCGGCCTTCT TTATGGCCTC ACCACGTCCC TCAGTCGTGG CCTAGCCCTC 
GCTCCCCAGC TCTGGGCCGC CCGTTCCATG GCCACCCTGA ACCAGATGCA CCGCTTAGGC 
CCCCGGAAGG AACCCCCTAA GCGTCTGGGC CCCACAGAGG GCCGGCCCCA GCTGAAGGGT 
GTGGTGTTGC GCACATTCAT CCGAAAGCCG AAGAAGCCCA ACTCCGCCAA CCGCAAGTGC 
TGCCGAGTCC GCCTCAGCAC AGGCAAAGAG GCTGTCTGCT TCATCCCCGG GGAGGGCCAC 
ACCCTGCAGG AGCATCACGT GGTCCTCGTG GAGGGTGGTC GCACCCAGGA CCTGCCTGGG 
GTCAAACTCA AAGTTGTGAG GGGCAAGTAT GACTGCGGCC ACGTGCAAAA GAAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001347179.1
CDS	287..706
Translation	MSWPGLLYGLTTSLSRGLALAPQLWAARSMATLNQMHRLGPRKEPPKRLGPTEGRPQLKGVVLRTFIRKPKKPNSANRKCCRVRLSTGKEAVCFIPGEGHTLQEHHVVLVEGGRTQDLPGVKLKVVRGKYDCGHVQKKK

Target ORF information:

RefSeq Version	NM_001360250.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mitochondrial ribosomal protein S12 (Mrps12), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001360250.1

ORF Insert Sequence:

ATGTCCTGGC CCGGCCTTCT TTATGGCCTC ACCACGTCCC TCAGTCGTGG CCTAGCCCTC 
GCTCCCCAGC TCTGGGCCGC CCGTTCCATG GCCACCCTGA ACCAGATGCA CCGCTTAGGC 
CCCCGGAAGG AACCCCCTAA GCGTCTGGGC CCCACAGAGG GCCGGCCCCA GCTGAAGGGT 
GTGGTGTTGC GCACATTCAT CCGAAAGCCG AAGAAGCCCA ACTCCGCCAA CCGCAAGTGC 
TGCCGAGTCC GCCTCAGCAC AGGCAAAGAG GCTGTCTGCT TCATCCCCGG GGAGGGCCAC 
ACCCTGCAGG AGCATCACGT GGTCCTCGTG GAGGGTGGTC GCACCCAGGA CCTGCCTGGG 
GTCAAACTCA AAGTTGTGAG GGGCAAGTAT GACTGCGGCC ACGTGCAAAA GAAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mrps12 ( NM_001360254.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001347183.1 Mus musculus mitochondrial ribosomal protein S12 (Mrps12), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OMu84139
Clone ID Related Accession (Same CDS sequence)	NM_001360254.1
Accession Version	NM_001360254.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 399bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-06
Organism	Mus musculus(house mouse)
Product	28S ribosomal protein S12, mitochondrial isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AA606858.1 and AC171210.1. Transcript Variant: This variant (4) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (b) has a shorter and distinct N-terminus compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AA606858.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849378, SAMN00849379 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTTCCTAG ACATTGCCCA GACATCAGGC CTAGCCCTCG CTCCCCAGCT CTGGGCCGCC 
CGTTCCATGG CCACCCTGAA CCAGATGCAC CGCTTAGGCC CCCGGAAGGA ACCCCCTAAG 
CGTCTGGGCC CCACAGAGGG CCGGCCCCAG CTGAAGGGTG TGGTGTTGCG CACATTCATC 
CGAAAGCCGA AGAAGCCCAA CTCCGCCAAC CGCAAGTGCT GCCGAGTCCG CCTCAGCACA 
GGCAAAGAGG CTGTCTGCTT CATCCCCGGG GAGGGCCACA CCCTGCAGGA GCATCACGTG 
GTCCTCGTGG AGGGTGGTCG CACCCAGGAC CTGCCTGGGG TCAAACTCAA AGTTGTGAGG 
GGCAAGTATG ACTGCGGCCA CGTGCAAAAG AAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001347183.1
CDS	80..478
Translation	MFLDIAQTSGLALAPQLWAARSMATLNQMHRLGPRKEPPKRLGPTEGRPQLKGVVLRTFIRKPKKPNSANRKCCRVRLSTGKEAVCFIPGEGHTLQEHHVVLVEGGRTQDLPGVKLKVVRGKYDCGHVQKKK

Target ORF information:

RefSeq Version	NM_001360254.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mitochondrial ribosomal protein S12 (Mrps12), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001360254.1

ORF Insert Sequence:

ATGTTCCTAG ACATTGCCCA GACATCAGGC CTAGCCCTCG CTCCCCAGCT CTGGGCCGCC 
CGTTCCATGG CCACCCTGAA CCAGATGCAC CGCTTAGGCC CCCGGAAGGA ACCCCCTAAG 
CGTCTGGGCC CCACAGAGGG CCGGCCCCAG CTGAAGGGTG TGGTGTTGCG CACATTCATC 
CGAAAGCCGA AGAAGCCCAA CTCCGCCAAC CGCAAGTGCT GCCGAGTCCG CCTCAGCACA 
GGCAAAGAGG CTGTCTGCTT CATCCCCGGG GAGGGCCACA CCCTGCAGGA GCATCACGTG 
GTCCTCGTGG AGGGTGGTCG CACCCAGGAC CTGCCTGGGG TCAAACTCAA AGTTGTGAGG 
GGCAAGTATG ACTGCGGCCA CGTGCAAAAG AAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mrps12 ( XM_006539908.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006539971.1 Mus musculus mitochondrial ribosomal protein S12 (Mrps12), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu13710
Clone ID Related Accession (Same CDS sequence)	NM_011885.5 , NM_001360252.1 , NM_011885.4 , XM_006539908.2 , XM_006539908.1 , NM_001360250.1
Accession Version	XM_006539908.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	28S ribosomal protein S12, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006539908.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTGGC CCGGCCTTCT TTATGGCCTC ACCACGTCCC TCAGTCGTGG CCTAGCCCTC 
GCTCCCCAGC TCTGGGCCGC CCGTTCCATG GCCACCCTGA ACCAGATGCA CCGCTTAGGC 
CCCCGGAAGG AACCCCCTAA GCGTCTGGGC CCCACAGAGG GCCGGCCCCA GCTGAAGGGT 
GTGGTGTTGC GCACATTCAT CCGAAAGCCG AAGAAGCCCA ACTCCGCCAA CCGCAAGTGC 
TGCCGAGTCC GCCTCAGCAC AGGCAAAGAG GCTGTCTGCT TCATCCCCGG GGAGGGCCAC 
ACCCTGCAGG AGCATCACGT GGTCCTCGTG GAGGGTGGTC GCACCCAGGA CCTGCCTGGG 
GTCAAACTCA AAGTTGTGAG GGGCAAGTAT GACTGCGGCC ACGTGCAAAA GAAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006539971.1
CDS	115..534
Translation	MSWPGLLYGLTTSLSRGLALAPQLWAARSMATLNQMHRLGPRKEPPKRLGPTEGRPQLKGVVLRTFIRKPKKPNSANRKCCRVRLSTGKEAVCFIPGEGHTLQEHHVVLVEGGRTQDLPGVKLKVVRGKYDCGHVQKKK

Target ORF information:

RefSeq Version	XM_006539908.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus mitochondrial ribosomal protein S12 (Mrps12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006539908.2

ORF Insert Sequence:

ATGTCCTGGC CCGGCCTTCT TTATGGCCTC ACCACGTCCC TCAGTCGTGG CCTAGCCCTC 
GCTCCCCAGC TCTGGGCCGC CCGTTCCATG GCCACCCTGA ACCAGATGCA CCGCTTAGGC 
CCCCGGAAGG AACCCCCTAA GCGTCTGGGC CCCACAGAGG GCCGGCCCCA GCTGAAGGGT 
GTGGTGTTGC GCACATTCAT CCGAAAGCCG AAGAAGCCCA ACTCCGCCAA CCGCAAGTGC 
TGCCGAGTCC GCCTCAGCAC AGGCAAAGAG GCTGTCTGCT TCATCCCCGG GGAGGGCCAC 
ACCCTGCAGG AGCATCACGT GGTCCTCGTG GAGGGTGGTC GCACCCAGGA CCTGCCTGGG 
GTCAAACTCA AAGTTGTGAG GGGCAAGTAT GACTGCGGCC ACGTGCAAAA GAAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mrps12 ( XM_006539908.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006539971.1 Mus musculus mitochondrial ribosomal protein S12 (Mrps12), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu13710
Clone ID Related Accession (Same CDS sequence)	NM_011885.5 , NM_001360252.1 , NM_011885.4 , XM_006539908.2 , XM_006539908.1 , NM_001360250.1
Accession Version	XM_006539908.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	28S ribosomal protein S12, mitochondrial isoform X1
Comment

ATGTCCTGGC CCGGCCTTCT TTATGGCCTC ACCACGTCCC TCAGTCGTGG CCTAGCCCTC 
GCTCCCCAGC TCTGGGCCGC CCGTTCCATG GCCACCCTGA ACCAGATGCA CCGCTTAGGC 
CCCCGGAAGG AACCCCCTAA GCGTCTGGGC CCCACAGAGG GCCGGCCCCA GCTGAAGGGT 
GTGGTGTTGC GCACATTCAT CCGAAAGCCG AAGAAGCCCA ACTCCGCCAA CCGCAAGTGC 
TGCCGAGTCC GCCTCAGCAC AGGCAAAGAG GCTGTCTGCT TCATCCCCGG GGAGGGCCAC 
ACCCTGCAGG AGCATCACGT GGTCCTCGTG GAGGGTGGTC GCACCCAGGA CCTGCCTGGG 
GTCAAACTCA AAGTTGTGAG GGGCAAGTAT GACTGCGGCC ACGTGCAAAA GAAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006539971.1
Translation	MSWPGLLYGLTTSLSRGLALAPQLWAARSMATLNQMHRLGPRKEPPKRLGPTEGRPQLKGVVLRTFIRKPKKPNSANRKCCRVRLSTGKEAVCFIPGEGHTLQEHHVVLVEGGRTQDLPGVKLKVVRGKYDCGHVQKKK

Target ORF information:

RefSeq Version	XM_006539908.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus mitochondrial ribosomal protein S12 (Mrps12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006539908.1

ORF Insert Sequence:

ATGTCCTGGC CCGGCCTTCT TTATGGCCTC ACCACGTCCC TCAGTCGTGG CCTAGCCCTC 
GCTCCCCAGC TCTGGGCCGC CCGTTCCATG GCCACCCTGA ACCAGATGCA CCGCTTAGGC 
CCCCGGAAGG AACCCCCTAA GCGTCTGGGC CCCACAGAGG GCCGGCCCCA GCTGAAGGGT 
GTGGTGTTGC GCACATTCAT CCGAAAGCCG AAGAAGCCCA ACTCCGCCAA CCGCAAGTGC 
TGCCGAGTCC GCCTCAGCAC AGGCAAAGAG GCTGTCTGCT TCATCCCCGG GGAGGGCCAC 
ACCCTGCAGG AGCATCACGT GGTCCTCGTG GAGGGTGGTC GCACCCAGGA CCTGCCTGGG 
GTCAAACTCA AAGTTGTGAG GGGCAAGTAT GACTGCGGCC ACGTGCAAAA GAAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Decreased expression levels of Ifi genes is associated to the increased resistance to spontaneous arthritis disease in mice deficiency of IL-1RA.
BMC immunology17(1)25(2016 08)
Liu X,Jiao Y,Cao Y,Deng N,Ma Y,Hasty KA,Kang A,Chen H,Stuart JM,Gu W

NF-Y influences directionality of transcription from the bidirectional Mrps12/Sarsm promoter in both mouse and human cells.
Biochimica et biophysica acta1789(5)432-42(2009 May)
Zanotto E,H?kkinen A,Teku G,Shen B,Ribeiro AS,Jacobs HT

The bidirectional promoter of two genes for the mitochondrial translational apparatus in mouse is regulated by an array of CCAAT boxes interacting with the transcription factor NF-Y.
Nucleic acids research35(2)664-77(2007)
Zanotto E,Shah ZH,Jacobs HT

Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria.
Cell115(5)629-40(2003 Nov)
Mootha VK,Bunkenborg J,Olsen JV,Hjerrild M,Wisniewski JR,Stahl E,Bolouri MS,Ray HN,Sihag S,Kamal M,Patterson N,Lander ES,Mann M

Proteomic analysis of the mammalian mitochondrial ribosome. Identification of protein components in the 28 S small subunit.
The Journal of biological chemistry276(35)33181-95(2001 Aug)
Suzuki T,Terasaki M,Takemoto-Hori C,Hanada T,Ueda T,Wada A,Watanabe K

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Mrps12 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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