Mdc1 gene cDNA ORF clone, Mus musculus(house mouse)

mRNA	Protein	Name
XM_006524262.3	XP_006524325.1	mediator of DNA damage checkpoint protein 1 isoform X2
NM_001010833.2	NP_001010833.2	mediator of DNA damage checkpoint protein 1
XM_006524260.1	XP_006524323.1	mediator of DNA damage checkpoint protein 1 isoform X1
XM_006524263.3	XP_006524326.1	mediator of DNA damage checkpoint protein 1 isoform X3
XM_006524262.4	XP_006524325.1	mediator of DNA damage checkpoint protein 1 isoform X2
XM_030249799.1	XP_030105659.1	mediator of DNA damage checkpoint protein 1 isoform X2
XM_006524264.4	XP_006524327.1	mediator of DNA damage checkpoint protein 1 isoform X4
XM_006524264.3	XP_006524327.1	mediator of DNA damage checkpoint protein 1 isoform X4
XM_006524260.2	XP_006524323.1	mediator of DNA damage checkpoint protein 1 isoform X1
XM_006524261.3	XP_006524324.1	mediator of DNA damage checkpoint protein 1 isoform X1
XM_006524263.4	XP_006524326.1	mediator of DNA damage checkpoint protein 1 isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu19129	XM_006524260.2 Latest version!	Mus musculus mediator of DNA damage checkpoint 1 (Mdc1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu53578	XM_006524262.4 Latest version!	Mus musculus mediator of DNA damage checkpoint 1 (Mdc1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu53578	XM_030249799.1 Latest version!	Mus musculus mediator of DNA damage checkpoint 1 (Mdc1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu53579	XM_006524263.4 Latest version!	Mus musculus mediator of DNA damage checkpoint 1 (Mdc1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu53580	XM_006524264.4 Latest version!	Mus musculus mediator of DNA damage checkpoint 1 (Mdc1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu19129	NM_001010833.2 Latest version!	Mus musculus mediator of DNA damage checkpoint 1 (Mdc1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OMu19129	XM_006524261.3 Latest version!	Mus musculus mediator of DNA damage checkpoint 1 (Mdc1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
View Old Accession Versions >>
OMu53578	XM_006524262.3	Mus musculus mediator of DNA damage checkpoint 1 (Mdc1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OMu53580	XM_006524264.3	Mus musculus mediator of DNA damage checkpoint 1 (Mdc1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OMu19129	XM_006524260.1	Mus musculus mediator of DNA damage checkpoint 1 (Mdc1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OMu53579	XM_006524263.3	Mus musculus mediator of DNA damage checkpoint 1 (Mdc1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
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Mdc1 ( XM_006524260.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524323.1 Mus musculus mediator of DNA damage checkpoint 1 (Mdc1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu19129
Clone ID Related Accession (Same CDS sequence)	NM_001010833.2 , XM_006524260.1 , XM_006524260.2 , XM_006524261.3
Accession Version	XM_006524260.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	mediator of DNA damage checkpoint protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006524260.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGCA CCCAGGTGAT TGACTGGGAT GCTGAAGAAG AGGAAGAGAC AGAACTATCC 
AGTGGCTCCT TGGGGTATAG TGTGGAGCCT ATAGGGCAGC TACGTCTCTT CAGTGGAACT 
CATGGACCAG AAAGAGATTT CCCCCTCTAC CTTGGCAAGA ATGTAGTTGG TCGAAGCCCT 
GACTGCTCTG TGGCCCTGCC TTTTCCATCC ATCTCCAAAC AGCATGCAGT AATTGAAATC 
TCAGCTTGGA ACAAAGCCCC TATCCTCCAG GATTGTGGGA GCCTCAATGG CACTCAAATC 
GTAAAGCCTC CTAGGGTCCT ACCTCCTGGA GTGAGCCATC GTCTGAGGGA CCAGGAATTA 
ATTCTGTTTG CAGATTTTCC CTGCCAGTAC CATCGCCTGG ATGTCCCACC ACCCTTGGTC 
CCTCGGAGTC TTCTAACTAT AGAGAAAACC CCCAGAATAC GGATAGAATC CCAAAATTCC 
AGAGTTTTGT TGGCTGCCGA TTCAGAGGAA GAAGGGGATT TTCCTTCTGG AAGGTGTGTG 
GCGAATGGAC AAAGGAATAC AGCATCCCCT TCAGCAACAG TAGTTCCAGA AAGTGATGAA 
GAGGTCTCTT CTCCGGCCCC AAGTGTCCCC GGGCCATCTT CACCCTTTGG TTTGGGCAGT 
GACACTGATG AAGAACAAGG TCAGCAACCA GGAGTAGAGG AGTCCTCTTT AGCTGACAGC 
AGTGGTGCTG CAGGGGAAGC TGAGCAGCCT GAAGCTAACG GAACGACAGC TGGCATACAG 
GCTCAGCCTA CTGAGCACAA ATTGAAGGAC ACAAAAGTCA AGAAGGAGGC AGGCAGGGCA 
GGGGTCTCAG ATGGGTCAGT TCTGGAGAGG AGCCCCACTC TTGGAGAGGA CAGTGACACA 
GAAGTGGACG AAGATCACAA ACCTGGGTTT GCGGACAGTG AGACAGATGT GGAAGAAGAG 
AGGATCCCTG TGACCCCCCC TGTAGCCCCT GTGAAGAAAA ACCAAGTGCT ACTTGCAGTT 
GGTATAGGAG ATCCTGAAGC ACCTGGTGTG GCACATCTGC AGGACTGCCT AGCTGGTAGT 
GGCACAGATG TGGAGGACAA GACTGCACTG GATGTCCCTC TAGAGAGAAA CCACACACCC 
ATGGTGATCA ACAGTGATAC AGATGAGGAG GAGGAGGAGG AGGAGGAGGA GGTCTCAGCA 
GCACTCACTT TGGCCCATCT GAAAGAGAGA GGAATTGGTT TGTGGAGTAG AGACCCAGGC 
GCAGAAGAGG TCAAGTCCCA ACCACAGGTC CTTGTAGAAC AAAGCCAGAG TGCCTCTGGG 
AGAGACAGTG ACACGGATGT GGAGGAGGAA TCCTCAGGGC GAAAGAGAGA GATTATCCCA 
GACAGTCCCA TGGATGTAGA TGAGGCTCTT ACTGTCACAC AGCCAGAGAG CCAGCCTCCC 
CGTAGGCCCA ATGATGCAGA TGAGTACATG GATATGAGCT CCCCTGGCAG CCATCTAGTG 
GTAAATCAGG CCTCCTTTGC TGTGGTAGGC AAGACCAGAG CACAAGTGGA GGAGGAAGTC 
CCAGGGCCAT CTGTTATCCT GGGGGAGAAG CACCAGGTGC CTCTAGAGGG AGCCCAGCCT 
CCTGAGGAAG CCTGGGAAAC AGCTGTTCAG GAAGGCTCAT CCTCACCAGA GGCAGCAGCC 
TCTGTAAGGC CTAGCCAGCA GCCAGTAGCA GAAGATGCTG GGACAGAGTG TGCTACAGCT 
GTTTCTGAGC AGGAAAGTAC TCTTGAGGTG AGGTCCCAAA GCGGGTCACC TGCAGCACCA 
GTGGAGCAAG TGGTGATACA TACAGATACT TCAGGGGATC CCACCCTGCC ACAGAGAGAG 
GGAGCCCAAA CCCCCACAGG AAGGGAGAGA GAAGCACATG TGGGCAGGAC CAAGAGTGCC 
AAAGAGTGCT GTGATGCAGA ACCTGAAGAT CTGTGCCTTC CAGCTACCCA GTGCTTTGTG 
GAAGGGGAGA GCCAGCACCC AGAAGCTGTC CAGAGTTTGG AAAATGAGCC TACCCAACTC 
TTCCCATGTA CTCTTCCACA AGAGCCAGGA CCTTCCCATC TCAGCCTTCA GACTCCAGGT 
GCAGATACCT TGGATGTGCC TTGGGAAGTC TTGGCTACAC AGCCGTTCTG TCTAAGAGAA 
CAGTCAGAGA CTTCTGAGCT TCATGAAGCT CATGGATCTC AACCATCTCT GCCTAGGGAA 
CCACCAGGAC ACCAACATCT GGTTCATACC AGCCCAGTTC ACACAGAGCT GTTGAGAATT 
GAAGGCAGAG AGATACAGAC TGTGGAGAAA GCCATGGGTA TACCAAAGGA AATGGCAGAC 
AGGATGACCC CTGAGAGAGA GCCATTGGAG AGGGAAATCA GGGGAAGAAC AGAAAACAGT 
GAGAGAGATG TGATAGGAGA AGAACTAATT CAGGGGACAA AGGACAGAGA GCCAAAGAAG 
GTGTTAGCTA GAGACAGTCA GAGAAAAGAG GCTGACAAGG ATCTTGAAGG GAATAGGGAG 
AGTTTGGAGG TAGAAATAGA GATGTCCAAA GACTCACAGA AGAGAGAGAG AAAAGTAGAG 
AAACCAGAAC CAAAGAGAGA ATGGGAGCCA GCTGATTTAG AAGTGACTCC AGATAGAGGG 
GTGACAGAGG AAGGGAGCCA TGATCAGAAA GGGCAAATTG CTAGTCTAAC ACTAAAGCCT 
GGAGTTGGGG TGAAGGACCT TGAGGGACTT GCATCAGCCC CAATAATCAC AGGGAGCCAG 
GCAGATGGAG GAAAGGGAGA CCCTTTGAGC CCTGGGAGGC AGCAGAGAGG CCGCTTGAGT 
TGCCAGACGA CACCTGCTGG AAAGGCTTCC AGGGGTGATC CAGAGCCCCC AGACCACTGC 
CTGTTTTCTT CAGTGCCTGA AGCTTCAACT CAGAGTCTCC TCACCTCTCA GAGCCAAAAG 
CAGTCTACAC CTCAGCCTCT GTTTTCGACC TCTTCTTCTG AGATACCCCT TCCTGAGAGT 
CTTCACACAA AGCCTAATGT CAGGCCACGG CGGTCCTCCA GGATGACCCC CTCCCCGCAT 
TCCTCTGCTG CCCTTAAGCC AAATACTACC TGCCCCACAA ACCAGCCTGC TGCCTCTAGA 
CCAACATCCC GACCCACTCG GGGCAGGGCA AATAGGTCCT CTACCAGGAC CCCAGAACTG 
ATTGTCCCTG TAGACCCTGA ACTCCAGCCT TCCACCTCCA CAGAACAGCC TGTCATCCCA 
AAACTTACGT CCCAGGTCAC TGAGGGCAGG GTACAGATGC CTGAACCACT TCTCACAGGT 
CCCGAGATCC AGTCTCCCAC CTCCACAGAA CAGTCTGTCA CCCCAGACCG CAAACCTCGG 
GCCACTCGGG GCAGGCCAAG TAAATCTCCC AACAAGACCC CAGAACCACT TATTTCTACT 
GGCCCTGAAC TCCAACCTCC CACCTCCATA GAACAACCTG TCATACCTAA ACCCACTTCT 
CGGGTCACTC GGGGCAGACC ACGTAAGTCT TCTGTCAGGA CCCCAGAATC AGTTGTCTCC 
ACTGGCCCTG AACTTCAGCC TCTCACCTCT ATAGAACAGC CTGTTATCCC TGAACCTAGG 
GCCACTCGAG GTAGACCAAG TAAGTCTTCT ATCAAGACCC CAGAATCAGT TGTCCCCACT 
GGTCCTGAGC TGCAGCCTCT TACCTCTGCA AAACAGCCTG TCACCCCTAA CCTCACATCT 
CGGGCCTCTC GGGGTAGATC AAGTAAGTCT ATCAGGACCC CGGAACCAGT TGTCCAAACT 
GGCCCTGAAT TCCATCCATC CACCTCCACA GAACAGCCTG ATACCCGTGA GCCCTCATCT 
CAGGCTAGGA CACGTAGGTC TGCTGTCAAG ACTCCTGAAG CGAGTGTCCC CACAACCCCT 
GAACTCCAGC CTTTCACTTC TAAAAAACAG CCTGCCCCTA AGCCTACAGC TCTGGTCACT 
CAGGGAAGGA CATATAAACC CTCCACTGAA GATTGTGAAT CAGTTGGGCC AGTGGCCCCT 
GATTTTGAGC CTTCCACCTC TACAGACCAT CTTGTCACCC CTAAGGTTAC AGATCAGAGC 
TTAACACTAC AGTCTTCACC CCTAAGTGCT TCCCCAGTTT CCAGTACCCC TGATCTCAAG 
CCTCCTGTCC CCATAGCCCA GCCTGTTACC CCAGAGCCCA TTCCACAAGC CAATCACCAA 
AGGAAACGAA GGGCTGCTGG GAAGCAGGGT TCCCGCACAG TCCCTCTGGG TCATAAGTCT 
TACTCTGCCC TCTCTGAACC TGAGCCCCAA TCTTCAGCCA GCCAAAGCTC AGGAGCATCA 
GAAGCAGACT CACCCCGTCA AAAACGTCCC AGAAGACAAG CCTCCCAGAA AACTGTAGTC 
ATCAAGGAAG AACCTGTAGA AACAGAAGTG AAGGAAGAGC CTCAAGAGAC TGCAATTCCA 
ACACCAGAAA AGAGAAAGAG GGACCATGCA GAAGAAGTGA CCCAGGGAAA ACCAACTCGA 
AGCCGGCGAA CTAAACCTAA CCAAGAAACA GCCCCCAAGG TACTGTTCAC AGGAGTCATG 
GATTCTCGTG GCGAGCGTGC AGTGCTGGCT CTGGGAGGCA GTCTGGCCAG CTCAGTAAAT 
GAGGCCTCCC ACTTAGTTAC TGATCGCATC CGCAGGACAG TCAAGTTCTT GTGTGCCCTG 
GGGAAGGGAA TTCCCATCCT GTCCCTGAAC TGGCTGTACC AGTCCCGAAA GGCTGGTTGC 
TTCTTGCCGC CTGATGACTA CTTAGTGACT GATCCTGAGC AAGAGAAGAA CTTTAGCTTC 
AGCCTTCGGG ATTCCCTGTG CCGGGCTCGG GAACGAAGAC TGCTGGAGGA CTATGAGATT 
CATGTGACCC CTGGCGTGCA GCCACCCCCC CCTCAGATGG GCGAGATCAT CAGTTGCTGT 
GGAGGCACTT TCCTGCCCAG CATGCCTCAC TCCTATAAGC TTCACCGAGT CATCATAACT 
TGCACTGAAG ACCTACCACG CTGTGCTATT CCATCCCGCC TGGGGCTACC CCTCCTCTCT 
CCTGAGTTCC TCCTGACTGG AGTGCTGAAG CAGGAAGCCA CACCAGAGGC CTTTGTTCTC 
TCCAATTTGG AAATGTCATC TACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524323.1
CDS	96..5222
Translation	MESTQVIDWDAEEEEETELSSGSLGYSVEPIGQLRLFSGTHGPERDFPLYLGKNVVGRSPDCSVALPFPSISKQHAVIEISAWNKAPILQDCGSLNGTQIVKPPRVLPPGVSHRLRDQELILFADFPCQYHRLDVPPPLVPRSLLTIEKTPRIRIESQNSRVLLAADSEEEGDFPSGRCVANGQRNTASPSATVVPESDEEVSSPAPSVPGPSSPFGLGSDTDEEQGQQPGVEESSLADSSGAAGEAEQPEANGTTAGIQAQPTEHKLKDTKVKKEAGRAGVSDGSVLERSPTLGEDSDTEVDEDHKPGFADSETDVEEERIPVTPPVAPVKKNQVLLAVGIGDPEAPGVAHLQDCLAGSGTDVEDKTALDVPLERNHTPMVINSDTDEEEEEEEEEVSAALTLAHLKERGIGLWSRDPGAEEVKSQPQVLVEQSQSASGRDSDTDVEEESSGRKREIIPDSPMDVDEALTVTQPESQPPRRPNDADEYMDMSSPGSHLVVNQASFAVVGKTRAQVEEEVPGPSVILGEKHQVPLEGAQPPEEAWETAVQEGSSSPEAAASVRPSQQPVAEDAGTECATAVSEQESTLEVRSQSGSPAAPVEQVVIHTDTSGDPTLPQREGAQTPTGREREAHVGRTKSAKECCDAEPEDLCLPATQCFVEGESQHPEAVQSLENEPTQLFPCTLPQEPGPSHLSLQTPGADTLDVPWEVLATQPFCLREQSETSELHEAHGSQPSLPREPPGHQHLVHTSPVHTELLRIEGREIQTVEKAMGIPKEMADRMTPEREPLEREIRGRTENSERDVIGEELIQGTKDREPKKVLARDSQRKEADKDLEGNRESLEVEIEMSKDSQKRERKVEKPEPKREWEPADLEVTPDRGVTEEGSHDQKGQIASLTLKPGVGVKDLEGLASAPIITGSQADGGKGDPLSPGRQQRGRLSCQTTPAGKASRGDPEPPDHCLFSSVPEASTQSLLTSQSQKQSTPQPLFSTSSSEIPLPESLHTKPNVRPRRSSRMTPSPHSSAALKPNTTCPTNQPAASRPTSRPTRGRANRSSTRTPELIVPVDPELQPSTSTEQPVIPKLTSQVTEGRVQMPEPLLTGPEIQSPTSTEQSVTPDRKPRATRGRPSKSPNKTPEPLISTGPELQPPTSIEQPVIPKPTSRVTRGRPRKSSVRTPESVVSTGPELQPLTSIEQPVIPEPRATRGRPSKSSIKTPESVVPTGPELQPLTSAKQPVTPNLTSRASRGRSSKSIRTPEPVVQTGPEFHPSTSTEQPDTREPSSQARTRRSAVKTPEASVPTTPELQPFTSKKQPAPKPTALVTQGRTYKPSTEDCESVGPVAPDFEPSTSTDHLVTPKVTDQSLTLQSSPLSASPVSSTPDLKPPVPIAQPVTPEPIPQANHQRKRRAAGKQGSRTVPLGHKSYSALSEPEPQSSASQSSGASEADSPRQKRPRRQASQKTVVIKEEPVETEVKEEPQETAIPTPEKRKRDHAEEVTQGKPTRSRRTKPNQETAPKVLFTGVMDSRGERAVLALGGSLASSVNEASHLVTDRIRRTVKFLCALGKGIPILSLNWLYQSRKAGCFLPPDDYLVTDPEQEKNFSFSLRDSLCRARERRLLEDYEIHVTPGVQPPPPQMGEIISCCGGTFLPSMPHSYKLHRVIITCTEDLPRCAIPSRLGLPLLSPEFLLTGVLKQEATPEAFVLSNLEMSST

Target ORF information:

RefSeq Version	XM_006524260.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus mediator of DNA damage checkpoint 1 (Mdc1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524260.2

ORF Insert Sequence:

ATGGAAAGCA CCCAGGTGAT TGACTGGGAT GCTGAAGAAG AGGAAGAGAC AGAACTATCC 
AGTGGCTCCT TGGGGTATAG TGTGGAGCCT ATAGGGCAGC TACGTCTCTT CAGTGGAACT 
CATGGACCAG AAAGAGATTT CCCCCTCTAC CTTGGCAAGA ATGTAGTTGG TCGAAGCCCT 
GACTGCTCTG TGGCCCTGCC TTTTCCATCC ATCTCCAAAC AGCATGCAGT AATTGAAATC 
TCAGCTTGGA ACAAAGCCCC TATCCTCCAG GATTGTGGGA GCCTCAATGG CACTCAAATC 
GTAAAGCCTC CTAGGGTCCT ACCTCCTGGA GTGAGCCATC GTCTGAGGGA CCAGGAATTA 
ATTCTGTTTG CAGATTTTCC CTGCCAGTAC CATCGCCTGG ATGTCCCACC ACCCTTGGTC 
CCTCGGAGTC TTCTAACTAT AGAGAAAACC CCCAGAATAC GGATAGAATC CCAAAATTCC 
AGAGTTTTGT TGGCTGCCGA TTCAGAGGAA GAAGGGGATT TTCCTTCTGG AAGGTGTGTG 
GCGAATGGAC AAAGGAATAC AGCATCCCCT TCAGCAACAG TAGTTCCAGA AAGTGATGAA 
GAGGTCTCTT CTCCGGCCCC AAGTGTCCCC GGGCCATCTT CACCCTTTGG TTTGGGCAGT 
GACACTGATG AAGAACAAGG TCAGCAACCA GGAGTAGAGG AGTCCTCTTT AGCTGACAGC 
AGTGGTGCTG CAGGGGAAGC TGAGCAGCCT GAAGCTAACG GAACGACAGC TGGCATACAG 
GCTCAGCCTA CTGAGCACAA ATTGAAGGAC ACAAAAGTCA AGAAGGAGGC AGGCAGGGCA 
GGGGTCTCAG ATGGGTCAGT TCTGGAGAGG AGCCCCACTC TTGGAGAGGA CAGTGACACA 
GAAGTGGACG AAGATCACAA ACCTGGGTTT GCGGACAGTG AGACAGATGT GGAAGAAGAG 
AGGATCCCTG TGACCCCCCC TGTAGCCCCT GTGAAGAAAA ACCAAGTGCT ACTTGCAGTT 
GGTATAGGAG ATCCTGAAGC ACCTGGTGTG GCACATCTGC AGGACTGCCT AGCTGGTAGT 
GGCACAGATG TGGAGGACAA GACTGCACTG GATGTCCCTC TAGAGAGAAA CCACACACCC 
ATGGTGATCA ACAGTGATAC AGATGAGGAG GAGGAGGAGG AGGAGGAGGA GGTCTCAGCA 
GCACTCACTT TGGCCCATCT GAAAGAGAGA GGAATTGGTT TGTGGAGTAG AGACCCAGGC 
GCAGAAGAGG TCAAGTCCCA ACCACAGGTC CTTGTAGAAC AAAGCCAGAG TGCCTCTGGG 
AGAGACAGTG ACACGGATGT GGAGGAGGAA TCCTCAGGGC GAAAGAGAGA GATTATCCCA 
GACAGTCCCA TGGATGTAGA TGAGGCTCTT ACTGTCACAC AGCCAGAGAG CCAGCCTCCC 
CGTAGGCCCA ATGATGCAGA TGAGTACATG GATATGAGCT CCCCTGGCAG CCATCTAGTG 
GTAAATCAGG CCTCCTTTGC TGTGGTAGGC AAGACCAGAG CACAAGTGGA GGAGGAAGTC 
CCAGGGCCAT CTGTTATCCT GGGGGAGAAG CACCAGGTGC CTCTAGAGGG AGCCCAGCCT 
CCTGAGGAAG CCTGGGAAAC AGCTGTTCAG GAAGGCTCAT CCTCACCAGA GGCAGCAGCC 
TCTGTAAGGC CTAGCCAGCA GCCAGTAGCA GAAGATGCTG GGACAGAGTG TGCTACAGCT 
GTTTCTGAGC AGGAAAGTAC TCTTGAGGTG AGGTCCCAAA GCGGGTCACC TGCAGCACCA 
GTGGAGCAAG TGGTGATACA TACAGATACT TCAGGGGATC CCACCCTGCC ACAGAGAGAG 
GGAGCCCAAA CCCCCACAGG AAGGGAGAGA GAAGCACATG TGGGCAGGAC CAAGAGTGCC 
AAAGAGTGCT GTGATGCAGA ACCTGAAGAT CTGTGCCTTC CAGCTACCCA GTGCTTTGTG 
GAAGGGGAGA GCCAGCACCC AGAAGCTGTC CAGAGTTTGG AAAATGAGCC TACCCAACTC 
TTCCCATGTA CTCTTCCACA AGAGCCAGGA CCTTCCCATC TCAGCCTTCA GACTCCAGGT 
GCAGATACCT TGGATGTGCC TTGGGAAGTC TTGGCTACAC AGCCGTTCTG TCTAAGAGAA 
CAGTCAGAGA CTTCTGAGCT TCATGAAGCT CATGGATCTC AACCATCTCT GCCTAGGGAA 
CCACCAGGAC ACCAACATCT GGTTCATACC AGCCCAGTTC ACACAGAGCT GTTGAGAATT 
GAAGGCAGAG AGATACAGAC TGTGGAGAAA GCCATGGGTA TACCAAAGGA AATGGCAGAC 
AGGATGACCC CTGAGAGAGA GCCATTGGAG AGGGAAATCA GGGGAAGAAC AGAAAACAGT 
GAGAGAGATG TGATAGGAGA AGAACTAATT CAGGGGACAA AGGACAGAGA GCCAAAGAAG 
GTGTTAGCTA GAGACAGTCA GAGAAAAGAG GCTGACAAGG ATCTTGAAGG GAATAGGGAG 
AGTTTGGAGG TAGAAATAGA GATGTCCAAA GACTCACAGA AGAGAGAGAG AAAAGTAGAG 
AAACCAGAAC CAAAGAGAGA ATGGGAGCCA GCTGATTTAG AAGTGACTCC AGATAGAGGG 
GTGACAGAGG AAGGGAGCCA TGATCAGAAA GGGCAAATTG CTAGTCTAAC ACTAAAGCCT 
GGAGTTGGGG TGAAGGACCT TGAGGGACTT GCATCAGCCC CAATAATCAC AGGGAGCCAG 
GCAGATGGAG GAAAGGGAGA CCCTTTGAGC CCTGGGAGGC AGCAGAGAGG CCGCTTGAGT 
TGCCAGACGA CACCTGCTGG AAAGGCTTCC AGGGGTGATC CAGAGCCCCC AGACCACTGC 
CTGTTTTCTT CAGTGCCTGA AGCTTCAACT CAGAGTCTCC TCACCTCTCA GAGCCAAAAG 
CAGTCTACAC CTCAGCCTCT GTTTTCGACC TCTTCTTCTG AGATACCCCT TCCTGAGAGT 
CTTCACACAA AGCCTAATGT CAGGCCACGG CGGTCCTCCA GGATGACCCC CTCCCCGCAT 
TCCTCTGCTG CCCTTAAGCC AAATACTACC TGCCCCACAA ACCAGCCTGC TGCCTCTAGA 
CCAACATCCC GACCCACTCG GGGCAGGGCA AATAGGTCCT CTACCAGGAC CCCAGAACTG 
ATTGTCCCTG TAGACCCTGA ACTCCAGCCT TCCACCTCCA CAGAACAGCC TGTCATCCCA 
AAACTTACGT CCCAGGTCAC TGAGGGCAGG GTACAGATGC CTGAACCACT TCTCACAGGT 
CCCGAGATCC AGTCTCCCAC CTCCACAGAA CAGTCTGTCA CCCCAGACCG CAAACCTCGG 
GCCACTCGGG GCAGGCCAAG TAAATCTCCC AACAAGACCC CAGAACCACT TATTTCTACT 
GGCCCTGAAC TCCAACCTCC CACCTCCATA GAACAACCTG TCATACCTAA ACCCACTTCT 
CGGGTCACTC GGGGCAGACC ACGTAAGTCT TCTGTCAGGA CCCCAGAATC AGTTGTCTCC 
ACTGGCCCTG AACTTCAGCC TCTCACCTCT ATAGAACAGC CTGTTATCCC TGAACCTAGG 
GCCACTCGAG GTAGACCAAG TAAGTCTTCT ATCAAGACCC CAGAATCAGT TGTCCCCACT 
GGTCCTGAGC TGCAGCCTCT TACCTCTGCA AAACAGCCTG TCACCCCTAA CCTCACATCT 
CGGGCCTCTC GGGGTAGATC AAGTAAGTCT ATCAGGACCC CGGAACCAGT TGTCCAAACT 
GGCCCTGAAT TCCATCCATC CACCTCCACA GAACAGCCTG ATACCCGTGA GCCCTCATCT 
CAGGCTAGGA CACGTAGGTC TGCTGTCAAG ACTCCTGAAG CGAGTGTCCC CACAACCCCT 
GAACTCCAGC CTTTCACTTC TAAAAAACAG CCTGCCCCTA AGCCTACAGC TCTGGTCACT 
CAGGGAAGGA CATATAAACC CTCCACTGAA GATTGTGAAT CAGTTGGGCC AGTGGCCCCT 
GATTTTGAGC CTTCCACCTC TACAGACCAT CTTGTCACCC CTAAGGTTAC AGATCAGAGC 
TTAACACTAC AGTCTTCACC CCTAAGTGCT TCCCCAGTTT CCAGTACCCC TGATCTCAAG 
CCTCCTGTCC CCATAGCCCA GCCTGTTACC CCAGAGCCCA TTCCACAAGC CAATCACCAA 
AGGAAACGAA GGGCTGCTGG GAAGCAGGGT TCCCGCACAG TCCCTCTGGG TCATAAGTCT 
TACTCTGCCC TCTCTGAACC TGAGCCCCAA TCTTCAGCCA GCCAAAGCTC AGGAGCATCA 
GAAGCAGACT CACCCCGTCA AAAACGTCCC AGAAGACAAG CCTCCCAGAA AACTGTAGTC 
ATCAAGGAAG AACCTGTAGA AACAGAAGTG AAGGAAGAGC CTCAAGAGAC TGCAATTCCA 
ACACCAGAAA AGAGAAAGAG GGACCATGCA GAAGAAGTGA CCCAGGGAAA ACCAACTCGA 
AGCCGGCGAA CTAAACCTAA CCAAGAAACA GCCCCCAAGG TACTGTTCAC AGGAGTCATG 
GATTCTCGTG GCGAGCGTGC AGTGCTGGCT CTGGGAGGCA GTCTGGCCAG CTCAGTAAAT 
GAGGCCTCCC ACTTAGTTAC TGATCGCATC CGCAGGACAG TCAAGTTCTT GTGTGCCCTG 
GGGAAGGGAA TTCCCATCCT GTCCCTGAAC TGGCTGTACC AGTCCCGAAA GGCTGGTTGC 
TTCTTGCCGC CTGATGACTA CTTAGTGACT GATCCTGAGC AAGAGAAGAA CTTTAGCTTC 
AGCCTTCGGG ATTCCCTGTG CCGGGCTCGG GAACGAAGAC TGCTGGAGGA CTATGAGATT 
CATGTGACCC CTGGCGTGCA GCCACCCCCC CCTCAGATGG GCGAGATCAT CAGTTGCTGT 
GGAGGCACTT TCCTGCCCAG CATGCCTCAC TCCTATAAGC TTCACCGAGT CATCATAACT 
TGCACTGAAG ACCTACCACG CTGTGCTATT CCATCCCGCC TGGGGCTACC CCTCCTCTCT 
CCTGAGTTCC TCCTGACTGG AGTGCTGAAG CAGGAAGCCA CACCAGAGGC CTTTGTTCTC 
TCCAATTTGG AAATGTCATC TACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mdc1 ( XM_006524262.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524325.1 Mus musculus mediator of DNA damage checkpoint 1 (Mdc1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu53578
Clone ID Related Accession (Same CDS sequence)	XM_006524262.3 , XM_006524262.4 , XM_030249799.1
Accession Version	XM_006524262.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5124bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	mediator of DNA damage checkpoint protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006524262.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGCA CCCAGGTGAT TGACTGGGAT GCTGAAGAAG AGGAAGAGAC AGAACTATCC 
AGTGGCTCCT TGGGGTATAG TGTGGAGCCT ATAGGGCAGC TACGTCTCTT CAGTGGAACT 
CATGGACCAG AAAGAGATTT CCCCCTCTAC CTTGGCAAGA ATGTAGTTGG TCGAAGCCCT 
GACTGCTCTG TGGCCCTGCC TTTTCCATCC ATCTCCAAAC AGCATGCAGT AATTGAAATC 
TCAGCTTGGA ACAAAGCCCC TATCCTCCAG GATTGTGGGA GCCTCAATGG CACTCAAATC 
GTAAAGCCTC CTAGGGTCCT ACCTCCTGGA GTGAGCCATC GTCTGAGGGA CCAGGAATTA 
ATTCTGTTTG CAGATTTTCC CTGCCAGTAC CATCGCCTGG ATGTCCCACC ACCCTTGGTC 
CCTCGGAGTC TTCTAACTAT AGAGAAAACC CCCAGAATAC GGATAGAATC CCAAAATTCC 
AGAGTTTTGT TGGCTGCCGA TTCAGAGGAA GAAGGGGATT TTCCTTCTGG AAGGTGTGTG 
GCGAATGGAC AAAGGAATAC AGCATCCCCT TCAGCAACAG TAGTTCCAGA AAGTGATGAA 
GAGGTCTCTT CTCCGGCCCC AAGTGTCCCC GGGCCATCTT CACCCTTTGG TTTGGGCAGT 
GACACTGATG AAGAACAAGG TCAGCAACCA GGAGTAGAGG AGTCCTCTTT AGCTGACAGC 
AGTGGTGCTG CAGGGGAAGC TGAGCAGCCT GAAGCTAACG GAACGACAGC TGGCATACAG 
GCTCAGCCTA CTGAGCACAA ATTGAAGGAC ACAAAAGTCA AGAAGGAGGC AGGCAGGGCA 
GGGGTCTCAG ATGGGTCAGT TCTGGAGAGG AGCCCCACTC TTGGAGAGGA CAGTGACACA 
GAAGTGGACG AAGATCACAA ACCTGGGTTT GCGGACAGTG AGACAGATGT GGAAGAAGAG 
AGGATCCCTG TGACCCCCCC TGTAGCCCCT GTGAAGAAAA ACCAAGTGCT ACTTGCAGTT 
GGTATAGGAG ATCCTGAAGC ACCTGGTGTG GCACATCTGC AGGACTGCCT AGCTGGTAGT 
GGCACAGATG TGGAGGACAA GACTGCACTG GATGTCCCTC TAGAGAGAAA CCACACACCC 
ATGGTGATCA ACAGTGATAC AGATGAGGAG GAGGAGGAGG AGGAGGAGGA GGTCTCAGCA 
GCACTCACTT TGGCCCATCT GAAAGAGAGA GGAATTGGTT TGTGGAGTAG AGACCCAGGC 
GCAGAAGAGG TCAAGTCCCA ACCACAGGTC CTTGTAGAAC AAAGCCAGAG TGCCTCTGGG 
AGAGACAGTG ACACGGATGT GGAGGAGGAA TCCTCAGGGC GAAAGAGAGA GATTATCCCA 
GACAGTCCCA TGGATGTAGA TGAGGCTCTT ACTGTCACAC AGCCAGAGAG CCAGCCTCCC 
CGTAGGCCCA ATGATGCAGA TGAGTACATG GATATGAGCT CCCCTGGCAG CCATCTAGTG 
GTAAATCAGG CCTCCTTTGC TGTGGTAGGC AAGACCAGAG CACAAGTGGA GGAGGAAGTC 
CCAGGGCCAT CTGTTATCCT GGGGGAGAAG CACCAGGTGC CTCTAGAGGG AGCCCAGCCT 
CCTGAGGAAG CCTGGGAAAC AGCTGTTCAG GAAGGCTCAT CCTCACCAGA GGCAGCAGCC 
TCTGTAAGGC CTAGCCAGCA GCCAGTAGCA GAAGATGCTG GGACAGAGTG TGCTACAGCT 
GTTTCTGAGC AGGAAAGTAC TCTTGAGGTG AGGTCCCAAA GCGGGTCACC TGCAGCACCA 
GTGGAGCAAG TGGTGATACA TACAGATACT TCAGGGGATC CCACCCTGCC ACAGAGAGAG 
GGAGCCCAAA CCCCCACAGG AAGGGAGAGA GAAGCACATG TGGGCAGGAC CAAGAGTGCC 
AAAGAGTGCT GTGATGAACC TGAAGATCTG TGCCTTCCAG CTACCCAGTG CTTTGTGGAA 
GGGGAGAGCC AGCACCCAGA AGCTGTCCAG AGTTTGGAAA ATGAGCCTAC CCAACTCTTC 
CCATGTACTC TTCCACAAGA GCCAGGACCT TCCCATCTCA GCCTTCAGAC TCCAGGTGCA 
GATACCTTGG ATGTGCCTTG GGAAGTCTTG GCTACACAGC CGTTCTGTCT AAGAGAACAG 
TCAGAGACTT CTGAGCTTCA TGAAGCTCAT GGATCTCAAC CATCTCTGCC TAGGGAACCA 
CCAGGACACC AACATCTGGT TCATACCAGC CCAGTTCACA CAGAGCTGTT GAGAATTGAA 
GGCAGAGAGA TACAGACTGT GGAGAAAGCC ATGGGTATAC CAAAGGAAAT GGCAGACAGG 
ATGACCCCTG AGAGAGAGCC ATTGGAGAGG GAAATCAGGG GAAGAACAGA AAACAGTGAG 
AGAGATGTGA TAGGAGAAGA ACTAATTCAG GGGACAAAGG ACAGAGAGCC AAAGAAGGTG 
TTAGCTAGAG ACAGTCAGAG AAAAGAGGCT GACAAGGATC TTGAAGGGAA TAGGGAGAGT 
TTGGAGGTAG AAATAGAGAT GTCCAAAGAC TCACAGAAGA GAGAGAGAAA AGTAGAGAAA 
CCAGAACCAA AGAGAGAATG GGAGCCAGCT GATTTAGAAG TGACTCCAGA TAGAGGGGTG 
ACAGAGGAAG GGAGCCATGA TCAGAAAGGG CAAATTGCTA GTCTAACACT AAAGCCTGGA 
GTTGGGGTGA AGGACCTTGA GGGACTTGCA TCAGCCCCAA TAATCACAGG GAGCCAGGCA 
GATGGAGGAA AGGGAGACCC TTTGAGCCCT GGGAGGCAGC AGAGAGGCCG CTTGAGTTGC 
CAGACGACAC CTGCTGGAAA GGCTTCCAGG GGTGATCCAG AGCCCCCAGA CCACTGCCTG 
TTTTCTTCAG TGCCTGAAGC TTCAACTCAG AGTCTCCTCA CCTCTCAGAG CCAAAAGCAG 
TCTACACCTC AGCCTCTGTT TTCGACCTCT TCTTCTGAGA TACCCCTTCC TGAGAGTCTT 
CACACAAAGC CTAATGTCAG GCCACGGCGG TCCTCCAGGA TGACCCCCTC CCCGCATTCC 
TCTGCTGCCC TTAAGCCAAA TACTACCTGC CCCACAAACC AGCCTGCTGC CTCTAGACCA 
ACATCCCGAC CCACTCGGGG CAGGGCAAAT AGGTCCTCTA CCAGGACCCC AGAACTGATT 
GTCCCTGTAG ACCCTGAACT CCAGCCTTCC ACCTCCACAG AACAGCCTGT CATCCCAAAA 
CTTACGTCCC AGGTCACTGA GGGCAGGGTA CAGATGCCTG AACCACTTCT CACAGGTCCC 
GAGATCCAGT CTCCCACCTC CACAGAACAG TCTGTCACCC CAGACCGCAA ACCTCGGGCC 
ACTCGGGGCA GGCCAAGTAA ATCTCCCAAC AAGACCCCAG AACCACTTAT TTCTACTGGC 
CCTGAACTCC AACCTCCCAC CTCCATAGAA CAACCTGTCA TACCTAAACC CACTTCTCGG 
GTCACTCGGG GCAGACCACG TAAGTCTTCT GTCAGGACCC CAGAATCAGT TGTCTCCACT 
GGCCCTGAAC TTCAGCCTCT CACCTCTATA GAACAGCCTG TTATCCCTGA ACCTAGGGCC 
ACTCGAGGTA GACCAAGTAA GTCTTCTATC AAGACCCCAG AATCAGTTGT CCCCACTGGT 
CCTGAGCTGC AGCCTCTTAC CTCTGCAAAA CAGCCTGTCA CCCCTAACCT CACATCTCGG 
GCCTCTCGGG GTAGATCAAG TAAGTCTATC AGGACCCCGG AACCAGTTGT CCAAACTGGC 
CCTGAATTCC ATCCATCCAC CTCCACAGAA CAGCCTGATA CCCGTGAGCC CTCATCTCAG 
GCTAGGACAC GTAGGTCTGC TGTCAAGACT CCTGAAGCGA GTGTCCCCAC AACCCCTGAA 
CTCCAGCCTT TCACTTCTAA AAAACAGCCT GCCCCTAAGC CTACAGCTCT GGTCACTCAG 
GGAAGGACAT ATAAACCCTC CACTGAAGAT TGTGAATCAG TTGGGCCAGT GGCCCCTGAT 
TTTGAGCCTT CCACCTCTAC AGACCATCTT GTCACCCCTA AGGTTACAGA TCAGAGCTTA 
ACACTACAGT CTTCACCCCT AAGTGCTTCC CCAGTTTCCA GTACCCCTGA TCTCAAGCCT 
CCTGTCCCCA TAGCCCAGCC TGTTACCCCA GAGCCCATTC CACAAGCCAA TCACCAAAGG 
AAACGAAGGG CTGCTGGGAA GCAGGGTTCC CGCACAGTCC CTCTGGGTCA TAAGTCTTAC 
TCTGCCCTCT CTGAACCTGA GCCCCAATCT TCAGCCAGCC AAAGCTCAGG AGCATCAGAA 
GCAGACTCAC CCCGTCAAAA ACGTCCCAGA AGACAAGCCT CCCAGAAAAC TGTAGTCATC 
AAGGAAGAAC CTGTAGAAAC AGAAGTGAAG GAAGAGCCTC AAGAGACTGC AATTCCAACA 
CCAGAAAAGA GAAAGAGGGA CCATGCAGAA GAAGTGACCC AGGGAAAACC AACTCGAAGC 
CGGCGAACTA AACCTAACCA AGAAACAGCC CCCAAGGTAC TGTTCACAGG AGTCATGGAT 
TCTCGTGGCG AGCGTGCAGT GCTGGCTCTG GGAGGCAGTC TGGCCAGCTC AGTAAATGAG 
GCCTCCCACT TAGTTACTGA TCGCATCCGC AGGACAGTCA AGTTCTTGTG TGCCCTGGGG 
AAGGGAATTC CCATCCTGTC CCTGAACTGG CTGTACCAGT CCCGAAAGGC TGGTTGCTTC 
TTGCCGCCTG ATGACTACTT AGTGACTGAT CCTGAGCAAG AGAAGAACTT TAGCTTCAGC 
CTTCGGGATT CCCTGTGCCG GGCTCGGGAA CGAAGACTGC TGGAGGACTA TGAGATTCAT 
GTGACCCCTG GCGTGCAGCC ACCCCCCCCT CAGATGGGCG AGATCATCAG TTGCTGTGGA 
GGCACTTTCC TGCCCAGCAT GCCTCACTCC TATAAGCTTC ACCGAGTCAT CATAACTTGC 
ACTGAAGACC TACCACGCTG TGCTATTCCA TCCCGCCTGG GGCTACCCCT CCTCTCTCCT 
GAGTTCCTCC TGACTGGAGT GCTGAAGCAG GAAGCCACAC CAGAGGCCTT TGTTCTCTCC 
AATTTGGAAA TGTCATCTAC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524325.1
CDS	158..5281
Translation	MESTQVIDWDAEEEEETELSSGSLGYSVEPIGQLRLFSGTHGPERDFPLYLGKNVVGRSPDCSVALPFPSISKQHAVIEISAWNKAPILQDCGSLNGTQIVKPPRVLPPGVSHRLRDQELILFADFPCQYHRLDVPPPLVPRSLLTIEKTPRIRIESQNSRVLLAADSEEEGDFPSGRCVANGQRNTASPSATVVPESDEEVSSPAPSVPGPSSPFGLGSDTDEEQGQQPGVEESSLADSSGAAGEAEQPEANGTTAGIQAQPTEHKLKDTKVKKEAGRAGVSDGSVLERSPTLGEDSDTEVDEDHKPGFADSETDVEEERIPVTPPVAPVKKNQVLLAVGIGDPEAPGVAHLQDCLAGSGTDVEDKTALDVPLERNHTPMVINSDTDEEEEEEEEEVSAALTLAHLKERGIGLWSRDPGAEEVKSQPQVLVEQSQSASGRDSDTDVEEESSGRKREIIPDSPMDVDEALTVTQPESQPPRRPNDADEYMDMSSPGSHLVVNQASFAVVGKTRAQVEEEVPGPSVILGEKHQVPLEGAQPPEEAWETAVQEGSSSPEAAASVRPSQQPVAEDAGTECATAVSEQESTLEVRSQSGSPAAPVEQVVIHTDTSGDPTLPQREGAQTPTGREREAHVGRTKSAKECCDEPEDLCLPATQCFVEGESQHPEAVQSLENEPTQLFPCTLPQEPGPSHLSLQTPGADTLDVPWEVLATQPFCLREQSETSELHEAHGSQPSLPREPPGHQHLVHTSPVHTELLRIEGREIQTVEKAMGIPKEMADRMTPEREPLEREIRGRTENSERDVIGEELIQGTKDREPKKVLARDSQRKEADKDLEGNRESLEVEIEMSKDSQKRERKVEKPEPKREWEPADLEVTPDRGVTEEGSHDQKGQIASLTLKPGVGVKDLEGLASAPIITGSQADGGKGDPLSPGRQQRGRLSCQTTPAGKASRGDPEPPDHCLFSSVPEASTQSLLTSQSQKQSTPQPLFSTSSSEIPLPESLHTKPNVRPRRSSRMTPSPHSSAALKPNTTCPTNQPAASRPTSRPTRGRANRSSTRTPELIVPVDPELQPSTSTEQPVIPKLTSQVTEGRVQMPEPLLTGPEIQSPTSTEQSVTPDRKPRATRGRPSKSPNKTPEPLISTGPELQPPTSIEQPVIPKPTSRVTRGRPRKSSVRTPESVVSTGPELQPLTSIEQPVIPEPRATRGRPSKSSIKTPESVVPTGPELQPLTSAKQPVTPNLTSRASRGRSSKSIRTPEPVVQTGPEFHPSTSTEQPDTREPSSQARTRRSAVKTPEASVPTTPELQPFTSKKQPAPKPTALVTQGRTYKPSTEDCESVGPVAPDFEPSTSTDHLVTPKVTDQSLTLQSSPLSASPVSSTPDLKPPVPIAQPVTPEPIPQANHQRKRRAAGKQGSRTVPLGHKSYSALSEPEPQSSASQSSGASEADSPRQKRPRRQASQKTVVIKEEPVETEVKEEPQETAIPTPEKRKRDHAEEVTQGKPTRSRRTKPNQETAPKVLFTGVMDSRGERAVLALGGSLASSVNEASHLVTDRIRRTVKFLCALGKGIPILSLNWLYQSRKAGCFLPPDDYLVTDPEQEKNFSFSLRDSLCRARERRLLEDYEIHVTPGVQPPPPQMGEIISCCGGTFLPSMPHSYKLHRVIITCTEDLPRCAIPSRLGLPLLSPEFLLTGVLKQEATPEAFVLSNLEMSST

Target ORF information:

RefSeq Version	XM_006524262.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus mediator of DNA damage checkpoint 1 (Mdc1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524262.4

ORF Insert Sequence:

ATGGAAAGCA CCCAGGTGAT TGACTGGGAT GCTGAAGAAG AGGAAGAGAC AGAACTATCC 
AGTGGCTCCT TGGGGTATAG TGTGGAGCCT ATAGGGCAGC TACGTCTCTT CAGTGGAACT 
CATGGACCAG AAAGAGATTT CCCCCTCTAC CTTGGCAAGA ATGTAGTTGG TCGAAGCCCT 
GACTGCTCTG TGGCCCTGCC TTTTCCATCC ATCTCCAAAC AGCATGCAGT AATTGAAATC 
TCAGCTTGGA ACAAAGCCCC TATCCTCCAG GATTGTGGGA GCCTCAATGG CACTCAAATC 
GTAAAGCCTC CTAGGGTCCT ACCTCCTGGA GTGAGCCATC GTCTGAGGGA CCAGGAATTA 
ATTCTGTTTG CAGATTTTCC CTGCCAGTAC CATCGCCTGG ATGTCCCACC ACCCTTGGTC 
CCTCGGAGTC TTCTAACTAT AGAGAAAACC CCCAGAATAC GGATAGAATC CCAAAATTCC 
AGAGTTTTGT TGGCTGCCGA TTCAGAGGAA GAAGGGGATT TTCCTTCTGG AAGGTGTGTG 
GCGAATGGAC AAAGGAATAC AGCATCCCCT TCAGCAACAG TAGTTCCAGA AAGTGATGAA 
GAGGTCTCTT CTCCGGCCCC AAGTGTCCCC GGGCCATCTT CACCCTTTGG TTTGGGCAGT 
GACACTGATG AAGAACAAGG TCAGCAACCA GGAGTAGAGG AGTCCTCTTT AGCTGACAGC 
AGTGGTGCTG CAGGGGAAGC TGAGCAGCCT GAAGCTAACG GAACGACAGC TGGCATACAG 
GCTCAGCCTA CTGAGCACAA ATTGAAGGAC ACAAAAGTCA AGAAGGAGGC AGGCAGGGCA 
GGGGTCTCAG ATGGGTCAGT TCTGGAGAGG AGCCCCACTC TTGGAGAGGA CAGTGACACA 
GAAGTGGACG AAGATCACAA ACCTGGGTTT GCGGACAGTG AGACAGATGT GGAAGAAGAG 
AGGATCCCTG TGACCCCCCC TGTAGCCCCT GTGAAGAAAA ACCAAGTGCT ACTTGCAGTT 
GGTATAGGAG ATCCTGAAGC ACCTGGTGTG GCACATCTGC AGGACTGCCT AGCTGGTAGT 
GGCACAGATG TGGAGGACAA GACTGCACTG GATGTCCCTC TAGAGAGAAA CCACACACCC 
ATGGTGATCA ACAGTGATAC AGATGAGGAG GAGGAGGAGG AGGAGGAGGA GGTCTCAGCA 
GCACTCACTT TGGCCCATCT GAAAGAGAGA GGAATTGGTT TGTGGAGTAG AGACCCAGGC 
GCAGAAGAGG TCAAGTCCCA ACCACAGGTC CTTGTAGAAC AAAGCCAGAG TGCCTCTGGG 
AGAGACAGTG ACACGGATGT GGAGGAGGAA TCCTCAGGGC GAAAGAGAGA GATTATCCCA 
GACAGTCCCA TGGATGTAGA TGAGGCTCTT ACTGTCACAC AGCCAGAGAG CCAGCCTCCC 
CGTAGGCCCA ATGATGCAGA TGAGTACATG GATATGAGCT CCCCTGGCAG CCATCTAGTG 
GTAAATCAGG CCTCCTTTGC TGTGGTAGGC AAGACCAGAG CACAAGTGGA GGAGGAAGTC 
CCAGGGCCAT CTGTTATCCT GGGGGAGAAG CACCAGGTGC CTCTAGAGGG AGCCCAGCCT 
CCTGAGGAAG CCTGGGAAAC AGCTGTTCAG GAAGGCTCAT CCTCACCAGA GGCAGCAGCC 
TCTGTAAGGC CTAGCCAGCA GCCAGTAGCA GAAGATGCTG GGACAGAGTG TGCTACAGCT 
GTTTCTGAGC AGGAAAGTAC TCTTGAGGTG AGGTCCCAAA GCGGGTCACC TGCAGCACCA 
GTGGAGCAAG TGGTGATACA TACAGATACT TCAGGGGATC CCACCCTGCC ACAGAGAGAG 
GGAGCCCAAA CCCCCACAGG AAGGGAGAGA GAAGCACATG TGGGCAGGAC CAAGAGTGCC 
AAAGAGTGCT GTGATGAACC TGAAGATCTG TGCCTTCCAG CTACCCAGTG CTTTGTGGAA 
GGGGAGAGCC AGCACCCAGA AGCTGTCCAG AGTTTGGAAA ATGAGCCTAC CCAACTCTTC 
CCATGTACTC TTCCACAAGA GCCAGGACCT TCCCATCTCA GCCTTCAGAC TCCAGGTGCA 
GATACCTTGG ATGTGCCTTG GGAAGTCTTG GCTACACAGC CGTTCTGTCT AAGAGAACAG 
TCAGAGACTT CTGAGCTTCA TGAAGCTCAT GGATCTCAAC CATCTCTGCC TAGGGAACCA 
CCAGGACACC AACATCTGGT TCATACCAGC CCAGTTCACA CAGAGCTGTT GAGAATTGAA 
GGCAGAGAGA TACAGACTGT GGAGAAAGCC ATGGGTATAC CAAAGGAAAT GGCAGACAGG 
ATGACCCCTG AGAGAGAGCC ATTGGAGAGG GAAATCAGGG GAAGAACAGA AAACAGTGAG 
AGAGATGTGA TAGGAGAAGA ACTAATTCAG GGGACAAAGG ACAGAGAGCC AAAGAAGGTG 
TTAGCTAGAG ACAGTCAGAG AAAAGAGGCT GACAAGGATC TTGAAGGGAA TAGGGAGAGT 
TTGGAGGTAG AAATAGAGAT GTCCAAAGAC TCACAGAAGA GAGAGAGAAA AGTAGAGAAA 
CCAGAACCAA AGAGAGAATG GGAGCCAGCT GATTTAGAAG TGACTCCAGA TAGAGGGGTG 
ACAGAGGAAG GGAGCCATGA TCAGAAAGGG CAAATTGCTA GTCTAACACT AAAGCCTGGA 
GTTGGGGTGA AGGACCTTGA GGGACTTGCA TCAGCCCCAA TAATCACAGG GAGCCAGGCA 
GATGGAGGAA AGGGAGACCC TTTGAGCCCT GGGAGGCAGC AGAGAGGCCG CTTGAGTTGC 
CAGACGACAC CTGCTGGAAA GGCTTCCAGG GGTGATCCAG AGCCCCCAGA CCACTGCCTG 
TTTTCTTCAG TGCCTGAAGC TTCAACTCAG AGTCTCCTCA CCTCTCAGAG CCAAAAGCAG 
TCTACACCTC AGCCTCTGTT TTCGACCTCT TCTTCTGAGA TACCCCTTCC TGAGAGTCTT 
CACACAAAGC CTAATGTCAG GCCACGGCGG TCCTCCAGGA TGACCCCCTC CCCGCATTCC 
TCTGCTGCCC TTAAGCCAAA TACTACCTGC CCCACAAACC AGCCTGCTGC CTCTAGACCA 
ACATCCCGAC CCACTCGGGG CAGGGCAAAT AGGTCCTCTA CCAGGACCCC AGAACTGATT 
GTCCCTGTAG ACCCTGAACT CCAGCCTTCC ACCTCCACAG AACAGCCTGT CATCCCAAAA 
CTTACGTCCC AGGTCACTGA GGGCAGGGTA CAGATGCCTG AACCACTTCT CACAGGTCCC 
GAGATCCAGT CTCCCACCTC CACAGAACAG TCTGTCACCC CAGACCGCAA ACCTCGGGCC 
ACTCGGGGCA GGCCAAGTAA ATCTCCCAAC AAGACCCCAG AACCACTTAT TTCTACTGGC 
CCTGAACTCC AACCTCCCAC CTCCATAGAA CAACCTGTCA TACCTAAACC CACTTCTCGG 
GTCACTCGGG GCAGACCACG TAAGTCTTCT GTCAGGACCC CAGAATCAGT TGTCTCCACT 
GGCCCTGAAC TTCAGCCTCT CACCTCTATA GAACAGCCTG TTATCCCTGA ACCTAGGGCC 
ACTCGAGGTA GACCAAGTAA GTCTTCTATC AAGACCCCAG AATCAGTTGT CCCCACTGGT 
CCTGAGCTGC AGCCTCTTAC CTCTGCAAAA CAGCCTGTCA CCCCTAACCT CACATCTCGG 
GCCTCTCGGG GTAGATCAAG TAAGTCTATC AGGACCCCGG AACCAGTTGT CCAAACTGGC 
CCTGAATTCC ATCCATCCAC CTCCACAGAA CAGCCTGATA CCCGTGAGCC CTCATCTCAG 
GCTAGGACAC GTAGGTCTGC TGTCAAGACT CCTGAAGCGA GTGTCCCCAC AACCCCTGAA 
CTCCAGCCTT TCACTTCTAA AAAACAGCCT GCCCCTAAGC CTACAGCTCT GGTCACTCAG 
GGAAGGACAT ATAAACCCTC CACTGAAGAT TGTGAATCAG TTGGGCCAGT GGCCCCTGAT 
TTTGAGCCTT CCACCTCTAC AGACCATCTT GTCACCCCTA AGGTTACAGA TCAGAGCTTA 
ACACTACAGT CTTCACCCCT AAGTGCTTCC CCAGTTTCCA GTACCCCTGA TCTCAAGCCT 
CCTGTCCCCA TAGCCCAGCC TGTTACCCCA GAGCCCATTC CACAAGCCAA TCACCAAAGG 
AAACGAAGGG CTGCTGGGAA GCAGGGTTCC CGCACAGTCC CTCTGGGTCA TAAGTCTTAC 
TCTGCCCTCT CTGAACCTGA GCCCCAATCT TCAGCCAGCC AAAGCTCAGG AGCATCAGAA 
GCAGACTCAC CCCGTCAAAA ACGTCCCAGA AGACAAGCCT CCCAGAAAAC TGTAGTCATC 
AAGGAAGAAC CTGTAGAAAC AGAAGTGAAG GAAGAGCCTC AAGAGACTGC AATTCCAACA 
CCAGAAAAGA GAAAGAGGGA CCATGCAGAA GAAGTGACCC AGGGAAAACC AACTCGAAGC 
CGGCGAACTA AACCTAACCA AGAAACAGCC CCCAAGGTAC TGTTCACAGG AGTCATGGAT 
TCTCGTGGCG AGCGTGCAGT GCTGGCTCTG GGAGGCAGTC TGGCCAGCTC AGTAAATGAG 
GCCTCCCACT TAGTTACTGA TCGCATCCGC AGGACAGTCA AGTTCTTGTG TGCCCTGGGG 
AAGGGAATTC CCATCCTGTC CCTGAACTGG CTGTACCAGT CCCGAAAGGC TGGTTGCTTC 
TTGCCGCCTG ATGACTACTT AGTGACTGAT CCTGAGCAAG AGAAGAACTT TAGCTTCAGC 
CTTCGGGATT CCCTGTGCCG GGCTCGGGAA CGAAGACTGC TGGAGGACTA TGAGATTCAT 
GTGACCCCTG GCGTGCAGCC ACCCCCCCCT CAGATGGGCG AGATCATCAG TTGCTGTGGA 
GGCACTTTCC TGCCCAGCAT GCCTCACTCC TATAAGCTTC ACCGAGTCAT CATAACTTGC 
ACTGAAGACC TACCACGCTG TGCTATTCCA TCCCGCCTGG GGCTACCCCT CCTCTCTCCT 
GAGTTCCTCC TGACTGGAGT GCTGAAGCAG GAAGCCACAC CAGAGGCCTT TGTTCTCTCC 
AATTTGGAAA TGTCATCTAC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mdc1 ( XM_030249799.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105659.1 Mus musculus mediator of DNA damage checkpoint 1 (Mdc1), transcript variant X4, mRNA.

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CloneID	OMu53578
Clone ID Related Accession (Same CDS sequence)	XM_006524262.3 , XM_006524262.4 , XM_030249799.1
Accession Version	XM_030249799.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5124bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	mediator of DNA damage checkpoint protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGCA CCCAGGTGAT TGACTGGGAT GCTGAAGAAG AGGAAGAGAC AGAACTATCC 
AGTGGCTCCT TGGGGTATAG TGTGGAGCCT ATAGGGCAGC TACGTCTCTT CAGTGGAACT 
CATGGACCAG AAAGAGATTT CCCCCTCTAC CTTGGCAAGA ATGTAGTTGG TCGAAGCCCT 
GACTGCTCTG TGGCCCTGCC TTTTCCATCC ATCTCCAAAC AGCATGCAGT AATTGAAATC 
TCAGCTTGGA ACAAAGCCCC TATCCTCCAG GATTGTGGGA GCCTCAATGG CACTCAAATC 
GTAAAGCCTC CTAGGGTCCT ACCTCCTGGA GTGAGCCATC GTCTGAGGGA CCAGGAATTA 
ATTCTGTTTG CAGATTTTCC CTGCCAGTAC CATCGCCTGG ATGTCCCACC ACCCTTGGTC 
CCTCGGAGTC TTCTAACTAT AGAGAAAACC CCCAGAATAC GGATAGAATC CCAAAATTCC 
AGAGTTTTGT TGGCTGCCGA TTCAGAGGAA GAAGGGGATT TTCCTTCTGG AAGGTGTGTG 
GCGAATGGAC AAAGGAATAC AGCATCCCCT TCAGCAACAG TAGTTCCAGA AAGTGATGAA 
GAGGTCTCTT CTCCGGCCCC AAGTGTCCCC GGGCCATCTT CACCCTTTGG TTTGGGCAGT 
GACACTGATG AAGAACAAGG TCAGCAACCA GGAGTAGAGG AGTCCTCTTT AGCTGACAGC 
AGTGGTGCTG CAGGGGAAGC TGAGCAGCCT GAAGCTAACG GAACGACAGC TGGCATACAG 
GCTCAGCCTA CTGAGCACAA ATTGAAGGAC ACAAAAGTCA AGAAGGAGGC AGGCAGGGCA 
GGGGTCTCAG ATGGGTCAGT TCTGGAGAGG AGCCCCACTC TTGGAGAGGA CAGTGACACA 
GAAGTGGACG AAGATCACAA ACCTGGGTTT GCGGACAGTG AGACAGATGT GGAAGAAGAG 
AGGATCCCTG TGACCCCCCC TGTAGCCCCT GTGAAGAAAA ACCAAGTGCT ACTTGCAGTT 
GGTATAGGAG ATCCTGAAGC ACCTGGTGTG GCACATCTGC AGGACTGCCT AGCTGGTAGT 
GGCACAGATG TGGAGGACAA GACTGCACTG GATGTCCCTC TAGAGAGAAA CCACACACCC 
ATGGTGATCA ACAGTGATAC AGATGAGGAG GAGGAGGAGG AGGAGGAGGA GGTCTCAGCA 
GCACTCACTT TGGCCCATCT GAAAGAGAGA GGAATTGGTT TGTGGAGTAG AGACCCAGGC 
GCAGAAGAGG TCAAGTCCCA ACCACAGGTC CTTGTAGAAC AAAGCCAGAG TGCCTCTGGG 
AGAGACAGTG ACACGGATGT GGAGGAGGAA TCCTCAGGGC GAAAGAGAGA GATTATCCCA 
GACAGTCCCA TGGATGTAGA TGAGGCTCTT ACTGTCACAC AGCCAGAGAG CCAGCCTCCC 
CGTAGGCCCA ATGATGCAGA TGAGTACATG GATATGAGCT CCCCTGGCAG CCATCTAGTG 
GTAAATCAGG CCTCCTTTGC TGTGGTAGGC AAGACCAGAG CACAAGTGGA GGAGGAAGTC 
CCAGGGCCAT CTGTTATCCT GGGGGAGAAG CACCAGGTGC CTCTAGAGGG AGCCCAGCCT 
CCTGAGGAAG CCTGGGAAAC AGCTGTTCAG GAAGGCTCAT CCTCACCAGA GGCAGCAGCC 
TCTGTAAGGC CTAGCCAGCA GCCAGTAGCA GAAGATGCTG GGACAGAGTG TGCTACAGCT 
GTTTCTGAGC AGGAAAGTAC TCTTGAGGTG AGGTCCCAAA GCGGGTCACC TGCAGCACCA 
GTGGAGCAAG TGGTGATACA TACAGATACT TCAGGGGATC CCACCCTGCC ACAGAGAGAG 
GGAGCCCAAA CCCCCACAGG AAGGGAGAGA GAAGCACATG TGGGCAGGAC CAAGAGTGCC 
AAAGAGTGCT GTGATGAACC TGAAGATCTG TGCCTTCCAG CTACCCAGTG CTTTGTGGAA 
GGGGAGAGCC AGCACCCAGA AGCTGTCCAG AGTTTGGAAA ATGAGCCTAC CCAACTCTTC 
CCATGTACTC TTCCACAAGA GCCAGGACCT TCCCATCTCA GCCTTCAGAC TCCAGGTGCA 
GATACCTTGG ATGTGCCTTG GGAAGTCTTG GCTACACAGC CGTTCTGTCT AAGAGAACAG 
TCAGAGACTT CTGAGCTTCA TGAAGCTCAT GGATCTCAAC CATCTCTGCC TAGGGAACCA 
CCAGGACACC AACATCTGGT TCATACCAGC CCAGTTCACA CAGAGCTGTT GAGAATTGAA 
GGCAGAGAGA TACAGACTGT GGAGAAAGCC ATGGGTATAC CAAAGGAAAT GGCAGACAGG 
ATGACCCCTG AGAGAGAGCC ATTGGAGAGG GAAATCAGGG GAAGAACAGA AAACAGTGAG 
AGAGATGTGA TAGGAGAAGA ACTAATTCAG GGGACAAAGG ACAGAGAGCC AAAGAAGGTG 
TTAGCTAGAG ACAGTCAGAG AAAAGAGGCT GACAAGGATC TTGAAGGGAA TAGGGAGAGT 
TTGGAGGTAG AAATAGAGAT GTCCAAAGAC TCACAGAAGA GAGAGAGAAA AGTAGAGAAA 
CCAGAACCAA AGAGAGAATG GGAGCCAGCT GATTTAGAAG TGACTCCAGA TAGAGGGGTG 
ACAGAGGAAG GGAGCCATGA TCAGAAAGGG CAAATTGCTA GTCTAACACT AAAGCCTGGA 
GTTGGGGTGA AGGACCTTGA GGGACTTGCA TCAGCCCCAA TAATCACAGG GAGCCAGGCA 
GATGGAGGAA AGGGAGACCC TTTGAGCCCT GGGAGGCAGC AGAGAGGCCG CTTGAGTTGC 
CAGACGACAC CTGCTGGAAA GGCTTCCAGG GGTGATCCAG AGCCCCCAGA CCACTGCCTG 
TTTTCTTCAG TGCCTGAAGC TTCAACTCAG AGTCTCCTCA CCTCTCAGAG CCAAAAGCAG 
TCTACACCTC AGCCTCTGTT TTCGACCTCT TCTTCTGAGA TACCCCTTCC TGAGAGTCTT 
CACACAAAGC CTAATGTCAG GCCACGGCGG TCCTCCAGGA TGACCCCCTC CCCGCATTCC 
TCTGCTGCCC TTAAGCCAAA TACTACCTGC CCCACAAACC AGCCTGCTGC CTCTAGACCA 
ACATCCCGAC CCACTCGGGG CAGGGCAAAT AGGTCCTCTA CCAGGACCCC AGAACTGATT 
GTCCCTGTAG ACCCTGAACT CCAGCCTTCC ACCTCCACAG AACAGCCTGT CATCCCAAAA 
CTTACGTCCC AGGTCACTGA GGGCAGGGTA CAGATGCCTG AACCACTTCT CACAGGTCCC 
GAGATCCAGT CTCCCACCTC CACAGAACAG TCTGTCACCC CAGACCGCAA ACCTCGGGCC 
ACTCGGGGCA GGCCAAGTAA ATCTCCCAAC AAGACCCCAG AACCACTTAT TTCTACTGGC 
CCTGAACTCC AACCTCCCAC CTCCATAGAA CAACCTGTCA TACCTAAACC CACTTCTCGG 
GTCACTCGGG GCAGACCACG TAAGTCTTCT GTCAGGACCC CAGAATCAGT TGTCTCCACT 
GGCCCTGAAC TTCAGCCTCT CACCTCTATA GAACAGCCTG TTATCCCTGA ACCTAGGGCC 
ACTCGAGGTA GACCAAGTAA GTCTTCTATC AAGACCCCAG AATCAGTTGT CCCCACTGGT 
CCTGAGCTGC AGCCTCTTAC CTCTGCAAAA CAGCCTGTCA CCCCTAACCT CACATCTCGG 
GCCTCTCGGG GTAGATCAAG TAAGTCTATC AGGACCCCGG AACCAGTTGT CCAAACTGGC 
CCTGAATTCC ATCCATCCAC CTCCACAGAA CAGCCTGATA CCCGTGAGCC CTCATCTCAG 
GCTAGGACAC GTAGGTCTGC TGTCAAGACT CCTGAAGCGA GTGTCCCCAC AACCCCTGAA 
CTCCAGCCTT TCACTTCTAA AAAACAGCCT GCCCCTAAGC CTACAGCTCT GGTCACTCAG 
GGAAGGACAT ATAAACCCTC CACTGAAGAT TGTGAATCAG TTGGGCCAGT GGCCCCTGAT 
TTTGAGCCTT CCACCTCTAC AGACCATCTT GTCACCCCTA AGGTTACAGA TCAGAGCTTA 
ACACTACAGT CTTCACCCCT AAGTGCTTCC CCAGTTTCCA GTACCCCTGA TCTCAAGCCT 
CCTGTCCCCA TAGCCCAGCC TGTTACCCCA GAGCCCATTC CACAAGCCAA TCACCAAAGG 
AAACGAAGGG CTGCTGGGAA GCAGGGTTCC CGCACAGTCC CTCTGGGTCA TAAGTCTTAC 
TCTGCCCTCT CTGAACCTGA GCCCCAATCT TCAGCCAGCC AAAGCTCAGG AGCATCAGAA 
GCAGACTCAC CCCGTCAAAA ACGTCCCAGA AGACAAGCCT CCCAGAAAAC TGTAGTCATC 
AAGGAAGAAC CTGTAGAAAC AGAAGTGAAG GAAGAGCCTC AAGAGACTGC AATTCCAACA 
CCAGAAAAGA GAAAGAGGGA CCATGCAGAA GAAGTGACCC AGGGAAAACC AACTCGAAGC 
CGGCGAACTA AACCTAACCA AGAAACAGCC CCCAAGGTAC TGTTCACAGG AGTCATGGAT 
TCTCGTGGCG AGCGTGCAGT GCTGGCTCTG GGAGGCAGTC TGGCCAGCTC AGTAAATGAG 
GCCTCCCACT TAGTTACTGA TCGCATCCGC AGGACAGTCA AGTTCTTGTG TGCCCTGGGG 
AAGGGAATTC CCATCCTGTC CCTGAACTGG CTGTACCAGT CCCGAAAGGC TGGTTGCTTC 
TTGCCGCCTG ATGACTACTT AGTGACTGAT CCTGAGCAAG AGAAGAACTT TAGCTTCAGC 
CTTCGGGATT CCCTGTGCCG GGCTCGGGAA CGAAGACTGC TGGAGGACTA TGAGATTCAT 
GTGACCCCTG GCGTGCAGCC ACCCCCCCCT CAGATGGGCG AGATCATCAG TTGCTGTGGA 
GGCACTTTCC TGCCCAGCAT GCCTCACTCC TATAAGCTTC ACCGAGTCAT CATAACTTGC 
ACTGAAGACC TACCACGCTG TGCTATTCCA TCCCGCCTGG GGCTACCCCT CCTCTCTCCT 
GAGTTCCTCC TGACTGGAGT GCTGAAGCAG GAAGCCACAC CAGAGGCCTT TGTTCTCTCC 
AATTTGGAAA TGTCATCTAC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105659.1
CDS	96..5219
Translation	MESTQVIDWDAEEEEETELSSGSLGYSVEPIGQLRLFSGTHGPERDFPLYLGKNVVGRSPDCSVALPFPSISKQHAVIEISAWNKAPILQDCGSLNGTQIVKPPRVLPPGVSHRLRDQELILFADFPCQYHRLDVPPPLVPRSLLTIEKTPRIRIESQNSRVLLAADSEEEGDFPSGRCVANGQRNTASPSATVVPESDEEVSSPAPSVPGPSSPFGLGSDTDEEQGQQPGVEESSLADSSGAAGEAEQPEANGTTAGIQAQPTEHKLKDTKVKKEAGRAGVSDGSVLERSPTLGEDSDTEVDEDHKPGFADSETDVEEERIPVTPPVAPVKKNQVLLAVGIGDPEAPGVAHLQDCLAGSGTDVEDKTALDVPLERNHTPMVINSDTDEEEEEEEEEVSAALTLAHLKERGIGLWSRDPGAEEVKSQPQVLVEQSQSASGRDSDTDVEEESSGRKREIIPDSPMDVDEALTVTQPESQPPRRPNDADEYMDMSSPGSHLVVNQASFAVVGKTRAQVEEEVPGPSVILGEKHQVPLEGAQPPEEAWETAVQEGSSSPEAAASVRPSQQPVAEDAGTECATAVSEQESTLEVRSQSGSPAAPVEQVVIHTDTSGDPTLPQREGAQTPTGREREAHVGRTKSAKECCDEPEDLCLPATQCFVEGESQHPEAVQSLENEPTQLFPCTLPQEPGPSHLSLQTPGADTLDVPWEVLATQPFCLREQSETSELHEAHGSQPSLPREPPGHQHLVHTSPVHTELLRIEGREIQTVEKAMGIPKEMADRMTPEREPLEREIRGRTENSERDVIGEELIQGTKDREPKKVLARDSQRKEADKDLEGNRESLEVEIEMSKDSQKRERKVEKPEPKREWEPADLEVTPDRGVTEEGSHDQKGQIASLTLKPGVGVKDLEGLASAPIITGSQADGGKGDPLSPGRQQRGRLSCQTTPAGKASRGDPEPPDHCLFSSVPEASTQSLLTSQSQKQSTPQPLFSTSSSEIPLPESLHTKPNVRPRRSSRMTPSPHSSAALKPNTTCPTNQPAASRPTSRPTRGRANRSSTRTPELIVPVDPELQPSTSTEQPVIPKLTSQVTEGRVQMPEPLLTGPEIQSPTSTEQSVTPDRKPRATRGRPSKSPNKTPEPLISTGPELQPPTSIEQPVIPKPTSRVTRGRPRKSSVRTPESVVSTGPELQPLTSIEQPVIPEPRATRGRPSKSSIKTPESVVPTGPELQPLTSAKQPVTPNLTSRASRGRSSKSIRTPEPVVQTGPEFHPSTSTEQPDTREPSSQARTRRSAVKTPEASVPTTPELQPFTSKKQPAPKPTALVTQGRTYKPSTEDCESVGPVAPDFEPSTSTDHLVTPKVTDQSLTLQSSPLSASPVSSTPDLKPPVPIAQPVTPEPIPQANHQRKRRAAGKQGSRTVPLGHKSYSALSEPEPQSSASQSSGASEADSPRQKRPRRQASQKTVVIKEEPVETEVKEEPQETAIPTPEKRKRDHAEEVTQGKPTRSRRTKPNQETAPKVLFTGVMDSRGERAVLALGGSLASSVNEASHLVTDRIRRTVKFLCALGKGIPILSLNWLYQSRKAGCFLPPDDYLVTDPEQEKNFSFSLRDSLCRARERRLLEDYEIHVTPGVQPPPPQMGEIISCCGGTFLPSMPHSYKLHRVIITCTEDLPRCAIPSRLGLPLLSPEFLLTGVLKQEATPEAFVLSNLEMSST

Target ORF information:

RefSeq Version	XM_030249799.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mediator of DNA damage checkpoint 1 (Mdc1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249799.1

ORF Insert Sequence:

ATGGAAAGCA CCCAGGTGAT TGACTGGGAT GCTGAAGAAG AGGAAGAGAC AGAACTATCC 
AGTGGCTCCT TGGGGTATAG TGTGGAGCCT ATAGGGCAGC TACGTCTCTT CAGTGGAACT 
CATGGACCAG AAAGAGATTT CCCCCTCTAC CTTGGCAAGA ATGTAGTTGG TCGAAGCCCT 
GACTGCTCTG TGGCCCTGCC TTTTCCATCC ATCTCCAAAC AGCATGCAGT AATTGAAATC 
TCAGCTTGGA ACAAAGCCCC TATCCTCCAG GATTGTGGGA GCCTCAATGG CACTCAAATC 
GTAAAGCCTC CTAGGGTCCT ACCTCCTGGA GTGAGCCATC GTCTGAGGGA CCAGGAATTA 
ATTCTGTTTG CAGATTTTCC CTGCCAGTAC CATCGCCTGG ATGTCCCACC ACCCTTGGTC 
CCTCGGAGTC TTCTAACTAT AGAGAAAACC CCCAGAATAC GGATAGAATC CCAAAATTCC 
AGAGTTTTGT TGGCTGCCGA TTCAGAGGAA GAAGGGGATT TTCCTTCTGG AAGGTGTGTG 
GCGAATGGAC AAAGGAATAC AGCATCCCCT TCAGCAACAG TAGTTCCAGA AAGTGATGAA 
GAGGTCTCTT CTCCGGCCCC AAGTGTCCCC GGGCCATCTT CACCCTTTGG TTTGGGCAGT 
GACACTGATG AAGAACAAGG TCAGCAACCA GGAGTAGAGG AGTCCTCTTT AGCTGACAGC 
AGTGGTGCTG CAGGGGAAGC TGAGCAGCCT GAAGCTAACG GAACGACAGC TGGCATACAG 
GCTCAGCCTA CTGAGCACAA ATTGAAGGAC ACAAAAGTCA AGAAGGAGGC AGGCAGGGCA 
GGGGTCTCAG ATGGGTCAGT TCTGGAGAGG AGCCCCACTC TTGGAGAGGA CAGTGACACA 
GAAGTGGACG AAGATCACAA ACCTGGGTTT GCGGACAGTG AGACAGATGT GGAAGAAGAG 
AGGATCCCTG TGACCCCCCC TGTAGCCCCT GTGAAGAAAA ACCAAGTGCT ACTTGCAGTT 
GGTATAGGAG ATCCTGAAGC ACCTGGTGTG GCACATCTGC AGGACTGCCT AGCTGGTAGT 
GGCACAGATG TGGAGGACAA GACTGCACTG GATGTCCCTC TAGAGAGAAA CCACACACCC 
ATGGTGATCA ACAGTGATAC AGATGAGGAG GAGGAGGAGG AGGAGGAGGA GGTCTCAGCA 
GCACTCACTT TGGCCCATCT GAAAGAGAGA GGAATTGGTT TGTGGAGTAG AGACCCAGGC 
GCAGAAGAGG TCAAGTCCCA ACCACAGGTC CTTGTAGAAC AAAGCCAGAG TGCCTCTGGG 
AGAGACAGTG ACACGGATGT GGAGGAGGAA TCCTCAGGGC GAAAGAGAGA GATTATCCCA 
GACAGTCCCA TGGATGTAGA TGAGGCTCTT ACTGTCACAC AGCCAGAGAG CCAGCCTCCC 
CGTAGGCCCA ATGATGCAGA TGAGTACATG GATATGAGCT CCCCTGGCAG CCATCTAGTG 
GTAAATCAGG CCTCCTTTGC TGTGGTAGGC AAGACCAGAG CACAAGTGGA GGAGGAAGTC 
CCAGGGCCAT CTGTTATCCT GGGGGAGAAG CACCAGGTGC CTCTAGAGGG AGCCCAGCCT 
CCTGAGGAAG CCTGGGAAAC AGCTGTTCAG GAAGGCTCAT CCTCACCAGA GGCAGCAGCC 
TCTGTAAGGC CTAGCCAGCA GCCAGTAGCA GAAGATGCTG GGACAGAGTG TGCTACAGCT 
GTTTCTGAGC AGGAAAGTAC TCTTGAGGTG AGGTCCCAAA GCGGGTCACC TGCAGCACCA 
GTGGAGCAAG TGGTGATACA TACAGATACT TCAGGGGATC CCACCCTGCC ACAGAGAGAG 
GGAGCCCAAA CCCCCACAGG AAGGGAGAGA GAAGCACATG TGGGCAGGAC CAAGAGTGCC 
AAAGAGTGCT GTGATGAACC TGAAGATCTG TGCCTTCCAG CTACCCAGTG CTTTGTGGAA 
GGGGAGAGCC AGCACCCAGA AGCTGTCCAG AGTTTGGAAA ATGAGCCTAC CCAACTCTTC 
CCATGTACTC TTCCACAAGA GCCAGGACCT TCCCATCTCA GCCTTCAGAC TCCAGGTGCA 
GATACCTTGG ATGTGCCTTG GGAAGTCTTG GCTACACAGC CGTTCTGTCT AAGAGAACAG 
TCAGAGACTT CTGAGCTTCA TGAAGCTCAT GGATCTCAAC CATCTCTGCC TAGGGAACCA 
CCAGGACACC AACATCTGGT TCATACCAGC CCAGTTCACA CAGAGCTGTT GAGAATTGAA 
GGCAGAGAGA TACAGACTGT GGAGAAAGCC ATGGGTATAC CAAAGGAAAT GGCAGACAGG 
ATGACCCCTG AGAGAGAGCC ATTGGAGAGG GAAATCAGGG GAAGAACAGA AAACAGTGAG 
AGAGATGTGA TAGGAGAAGA ACTAATTCAG GGGACAAAGG ACAGAGAGCC AAAGAAGGTG 
TTAGCTAGAG ACAGTCAGAG AAAAGAGGCT GACAAGGATC TTGAAGGGAA TAGGGAGAGT 
TTGGAGGTAG AAATAGAGAT GTCCAAAGAC TCACAGAAGA GAGAGAGAAA AGTAGAGAAA 
CCAGAACCAA AGAGAGAATG GGAGCCAGCT GATTTAGAAG TGACTCCAGA TAGAGGGGTG 
ACAGAGGAAG GGAGCCATGA TCAGAAAGGG CAAATTGCTA GTCTAACACT AAAGCCTGGA 
GTTGGGGTGA AGGACCTTGA GGGACTTGCA TCAGCCCCAA TAATCACAGG GAGCCAGGCA 
GATGGAGGAA AGGGAGACCC TTTGAGCCCT GGGAGGCAGC AGAGAGGCCG CTTGAGTTGC 
CAGACGACAC CTGCTGGAAA GGCTTCCAGG GGTGATCCAG AGCCCCCAGA CCACTGCCTG 
TTTTCTTCAG TGCCTGAAGC TTCAACTCAG AGTCTCCTCA CCTCTCAGAG CCAAAAGCAG 
TCTACACCTC AGCCTCTGTT TTCGACCTCT TCTTCTGAGA TACCCCTTCC TGAGAGTCTT 
CACACAAAGC CTAATGTCAG GCCACGGCGG TCCTCCAGGA TGACCCCCTC CCCGCATTCC 
TCTGCTGCCC TTAAGCCAAA TACTACCTGC CCCACAAACC AGCCTGCTGC CTCTAGACCA 
ACATCCCGAC CCACTCGGGG CAGGGCAAAT AGGTCCTCTA CCAGGACCCC AGAACTGATT 
GTCCCTGTAG ACCCTGAACT CCAGCCTTCC ACCTCCACAG AACAGCCTGT CATCCCAAAA 
CTTACGTCCC AGGTCACTGA GGGCAGGGTA CAGATGCCTG AACCACTTCT CACAGGTCCC 
GAGATCCAGT CTCCCACCTC CACAGAACAG TCTGTCACCC CAGACCGCAA ACCTCGGGCC 
ACTCGGGGCA GGCCAAGTAA ATCTCCCAAC AAGACCCCAG AACCACTTAT TTCTACTGGC 
CCTGAACTCC AACCTCCCAC CTCCATAGAA CAACCTGTCA TACCTAAACC CACTTCTCGG 
GTCACTCGGG GCAGACCACG TAAGTCTTCT GTCAGGACCC CAGAATCAGT TGTCTCCACT 
GGCCCTGAAC TTCAGCCTCT CACCTCTATA GAACAGCCTG TTATCCCTGA ACCTAGGGCC 
ACTCGAGGTA GACCAAGTAA GTCTTCTATC AAGACCCCAG AATCAGTTGT CCCCACTGGT 
CCTGAGCTGC AGCCTCTTAC CTCTGCAAAA CAGCCTGTCA CCCCTAACCT CACATCTCGG 
GCCTCTCGGG GTAGATCAAG TAAGTCTATC AGGACCCCGG AACCAGTTGT CCAAACTGGC 
CCTGAATTCC ATCCATCCAC CTCCACAGAA CAGCCTGATA CCCGTGAGCC CTCATCTCAG 
GCTAGGACAC GTAGGTCTGC TGTCAAGACT CCTGAAGCGA GTGTCCCCAC AACCCCTGAA 
CTCCAGCCTT TCACTTCTAA AAAACAGCCT GCCCCTAAGC CTACAGCTCT GGTCACTCAG 
GGAAGGACAT ATAAACCCTC CACTGAAGAT TGTGAATCAG TTGGGCCAGT GGCCCCTGAT 
TTTGAGCCTT CCACCTCTAC AGACCATCTT GTCACCCCTA AGGTTACAGA TCAGAGCTTA 
ACACTACAGT CTTCACCCCT AAGTGCTTCC CCAGTTTCCA GTACCCCTGA TCTCAAGCCT 
CCTGTCCCCA TAGCCCAGCC TGTTACCCCA GAGCCCATTC CACAAGCCAA TCACCAAAGG 
AAACGAAGGG CTGCTGGGAA GCAGGGTTCC CGCACAGTCC CTCTGGGTCA TAAGTCTTAC 
TCTGCCCTCT CTGAACCTGA GCCCCAATCT TCAGCCAGCC AAAGCTCAGG AGCATCAGAA 
GCAGACTCAC CCCGTCAAAA ACGTCCCAGA AGACAAGCCT CCCAGAAAAC TGTAGTCATC 
AAGGAAGAAC CTGTAGAAAC AGAAGTGAAG GAAGAGCCTC AAGAGACTGC AATTCCAACA 
CCAGAAAAGA GAAAGAGGGA CCATGCAGAA GAAGTGACCC AGGGAAAACC AACTCGAAGC 
CGGCGAACTA AACCTAACCA AGAAACAGCC CCCAAGGTAC TGTTCACAGG AGTCATGGAT 
TCTCGTGGCG AGCGTGCAGT GCTGGCTCTG GGAGGCAGTC TGGCCAGCTC AGTAAATGAG 
GCCTCCCACT TAGTTACTGA TCGCATCCGC AGGACAGTCA AGTTCTTGTG TGCCCTGGGG 
AAGGGAATTC CCATCCTGTC CCTGAACTGG CTGTACCAGT CCCGAAAGGC TGGTTGCTTC 
TTGCCGCCTG ATGACTACTT AGTGACTGAT CCTGAGCAAG AGAAGAACTT TAGCTTCAGC 
CTTCGGGATT CCCTGTGCCG GGCTCGGGAA CGAAGACTGC TGGAGGACTA TGAGATTCAT 
GTGACCCCTG GCGTGCAGCC ACCCCCCCCT CAGATGGGCG AGATCATCAG TTGCTGTGGA 
GGCACTTTCC TGCCCAGCAT GCCTCACTCC TATAAGCTTC ACCGAGTCAT CATAACTTGC 
ACTGAAGACC TACCACGCTG TGCTATTCCA TCCCGCCTGG GGCTACCCCT CCTCTCTCCT 
GAGTTCCTCC TGACTGGAGT GCTGAAGCAG GAAGCCACAC CAGAGGCCTT TGTTCTCTCC 
AATTTGGAAA TGTCATCTAC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mdc1 ( XM_006524263.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524326.1 Mus musculus mediator of DNA damage checkpoint 1 (Mdc1), transcript variant X5, mRNA.

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CloneID	OMu53579
Clone ID Related Accession (Same CDS sequence)	XM_006524263.3 , XM_006524263.4
Accession Version	XM_006524263.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	mediator of DNA damage checkpoint protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006524263.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGCA CCCAGGTGAT TGACTGGGAT GCTGAAGAAG AGGAAGAGAC AGAACTATCC 
AGTGGCTCCT TGGGGTATAG TGTGGAGCCT ATAGGGCAGC TACGTCTCTT CAGTGGAACT 
CATGGACCAG AAAGAGATTT CCCCCTCTAC CTTGGCAAGA ATGTAGTTGG TCGAAGCCCT 
GACTGCTCTG TGGCCCTGCC TTTTCCATCC ATCTCCAAAC AGCATGCAGT AATTGAAATC 
TCAGCTTGGA ACAAAGCCCC TATCCTCCAG GATTGTGGGA GCCTCAATGG CACTCAAATC 
GTAAAGCCTC CTAGGGTCCT ACCTCCTGGA GTGAGCCATC GTCTGAGGGA CCAGGAATTA 
ATTCTGTTTG CAGATTTTCC CTGCCAGTAC CATCGCCTGG ATGTCCCACC ACCCTTGGTC 
CCTCGGAGTC TTCTAACTAT AGAGAAAACC CCCAGAATAC GGATAGAATC CCAAAATTCC 
AGAGTTTTGT TGGCTGCCGA TTCAGAGGAA GAAGGGGATT TTCCTTCTGG AAGGTGTGTG 
GCGAATGGAC AAAGGAATAC AGCATCCCCT TCAGCAACAG TAGTTCCAGA AAGTGATGAA 
GAGGTCTCTT CTCCGGCCCC AAGTGTCCCC GGGCCATCTT CACCCTTTGG TTTGGGCAGT 
GACACTGATG AAGAACAAGG TCAGCAACCA GGAGTAGAGG AGTCCTCTTT AGCTGACAGC 
AGTGGTGCTG CAGGGGAAGC TGAGCAGCCT GAAGCTAACG GAACGACAGC TGGCATACAG 
GCTCAGCCTA CTGAGCACAA ATTGAAGGAC ACAAAAGTCA AGAAGGAGGC AGGCAGGGCA 
GGGGTCTCAG ATGGGTCAGT TCTGGAGAGG AGCCCCACTC TTGGAGAGGA CAGTGACACA 
GAAGTGGACG AAGATCACAA ACCTGGGTTT GCGGACAGTG AGACAGATGT GGAAGAAGAG 
AGGATCCCTG TGACCCCCCC TGTAGCCCCT GTGAAGAAAA ACCAAGTGCT ACTTGCAGTT 
GGTATAGGAG ATCCTGAAGC ACCTGGTGTG GCACATCTGC AGGACTGCCT AGCTGGTAGT 
GGCACAGATG TGGAGGACAA GACTGCACTG GATGTCCCTC TAGAGAGAAA CCACACACCC 
ATGGTGATCA ACAGTGATAC AGATGAGGAG GAGGAGGAGG AGGAGGAGGA GGTCTCAGCA 
GCACTCACTT TGGCCCATCT GAAAGAGAGA GGAATTGGTT TGTGGAGTAG AGACCCAGGC 
GCAGAAGAGG TCAAGTCCCA ACCACAGGTC CTTGTAGAAC AAAGCCAGAG TGCCTCTGGG 
AGAGACAGTG ACACGGATGT GGAGGAGGAA TCCTCAGGGC GAAAGAGAGA GATTATCCCA 
GACAGTCCCA TGGATGTAGA TGAGGCTCTT ACTGTCACAC AGCCAGAGAG CCAGCCTCCC 
CGTAGGCCCA ATGATGCAGA TGAGTACATG GATATGAGCT CCCCTGGCAG CCATCTAGTG 
GTAAATCAGG CCTCCTTTGC TGTGGTAGGC AAGACCAGAG CACAAGTGGA GGAGGAAGTC 
CCAGGGCCAT CTGTTATCCT GGGGGAGAAG CACCAGGTGC CTCTAGAGGG AGCCCAGCCT 
CCTGAGGAAG CCTGGGAAAC AGCTGTTCAG GAAGGCTCAT CCTCACCAGA GGCAGCAGCC 
TCTGTAAGGC CTAGCCAGCA GCCAGTAGCA GAAGATGCTG GGACAGAGTG TGCTACAGCT 
GTTTCTGAGC AGGAAAGTAC TCTTGAGGTG AGGTCCCAAA GCGGGTCACC TGCAGCACCA 
GTGGAGCAAG TGGTGATACA TACAGATACT TCAGGGGATC CCACCCTGCC ACAGAGAGAG 
GGAGCCCAAA CCCCCACAGG AAGGGAGAGA GAAGCACATG TGGGCAGGAC CAAGAGTGCC 
AAAGAGTGCT GTGATGCAGA ACCTGAAGAT CTGTGCCTTC CAGCTACCCA GTGCTTTGTG 
GAAGGGGAGA GCCAGCACCC AGAAGCTGTC CAGAGTTTGG AAAATGAGCC TACCCAACTC 
TTCCCATGTA CTCTTCCACA AGAGCCAGGA CCTTCCCATC TCAGCCTTCA GACTCCAGGT 
GCAGATACCT TGGATGTGCC TTGGGAAGTC TTGGCTACAC AGCCGTTCTG TCTAAGAGAA 
CAGTCAGAGA CTTCTGAGCT TCATGAAGCT CATGGATCTC AACCATCTCT GCCTAGGGAA 
CCACCAGGAC ACCAACATCT GGTTCATACC AGCCCAGTTC ACACAGAGCT GTTGAGAATT 
GAAGGCAGAG AGATACAGAC TGTGGAGAAA GCCATGGGCC GCTTGAGTTG CCAGACGACA 
CCTGCTGGAA AGGCTTCCAG GGGTGATCCA GAGCCCCCAG ACCACTGCCT GTTTTCTTCA 
GTGCCTGAAG CTTCAACTCA GAGTCTCCTC ACCTCTCAGA GCCAAAAGCA GTCTACACCT 
CAGCCTCTGT TTTCGACCTC TTCTTCTGAG ATACCCCTTC CTGAGAGTCT TCACACAAAG 
CCTAATGTCA GGCCACGGCG GTCCTCCAGG ATGACCCCCT CCCCGCATTC CTCTGCTGCC 
CTTAAGCCAA ATACTACCTG CCCCACAAAC CAGCCTGCTG CCTCTAGACC AACATCCCGA 
CCCACTCGGG GCAGGGCAAA TAGGTCCTCT ACCAGGACCC CAGAACTGAT TGTCCCTGTA 
GACCCTGAAC TCCAGCCTTC CACCTCCACA GAACAGCCTG TCATCCCAAA ACTTACGTCC 
CAGGTCACTG AGGGCAGGGT ACAGATGCCT GAACCACTTC TCACAGGTCC CGAGATCCAG 
TCTCCCACCT CCACAGAACA GTCTGTCACC CCAGACCGCA AACCTCGGGC CACTCGGGGC 
AGGCCAAGTA AATCTCCCAA CAAGACCCCA GAACCACTTA TTTCTACTGG CCCTGAACTC 
CAACCTCCCA CCTCCATAGA ACAACCTGTC ATACCTAAAC CCACTTCTCG GGTCACTCGG 
GGCAGACCAC GTAAGTCTTC TGTCAGGACC CCAGAATCAG TTGTCTCCAC TGGCCCTGAA 
CTTCAGCCTC TCACCTCTAT AGAACAGCCT GTTATCCCTG AACCTAGGGC CACTCGAGGT 
AGACCAAGTA AGTCTTCTAT CAAGACCCCA GAATCAGTTG TCCCCACTGG TCCTGAGCTG 
CAGCCTCTTA CCTCTGCAAA ACAGCCTGTC ACCCCTAACC TCACATCTCG GGCCTCTCGG 
GGTAGATCAA GTAAGTCTAT CAGGACCCCG GAACCAGTTG TCCAAACTGG CCCTGAATTC 
CATCCATCCA CCTCCACAGA ACAGCCTGAT ACCCGTGAGC CCTCATCTCA GGCTAGGACA 
CGTAGGTCTG CTGTCAAGAC TCCTGAAGCG AGTGTCCCCA CAACCCCTGA ACTCCAGCCT 
TTCACTTCTA AAAAACAGCC TGCCCCTAAG CCTACAGCTC TGGTCACTCA GGGAAGGACA 
TATAAACCCT CCACTGAAGA TTGTGAATCA GTTGGGCCAG TGGCCCCTGA TTTTGAGCCT 
TCCACCTCTA CAGACCATCT TGTCACCCCT AAGGTTACAG ATCAGAGCTT AACACTACAG 
TCTTCACCCC TAAGTGCTTC CCCAGTTTCC AGTACCCCTG ATCTCAAGCC TCCTGTCCCC 
ATAGCCCAGC CTGTTACCCC AGAGCCCATT CCACAAGCCA ATCACCAAAG GAAACGAAGG 
GCTGCTGGGA AGCAGGGTTC CCGCACAGTC CCTCTGGGTC ATAAGTCTTA CTCTGCCCTC 
TCTGAACCTG AGCCCCAATC TTCAGCCAGC CAAAGCTCAG GAGCATCAGA AGCAGACTCA 
CCCCGTCAAA AACGTCCCAG AAGACAAGCC TCCCAGAAAA CTGTAGTCAT CAAGGAAGAA 
CCTGTAGAAA CAGAAGTGAA GGAAGAGCCT CAAGAGACTG CAATTCCAAC ACCAGAAAAG 
AGAAAGAGGG ACCATGCAGA AGAAGTGACC CAGGGAAAAC CAACTCGAAG CCGGCGAACT 
AAACCTAACC AAGAAACAGC CCCCAAGGTA CTGTTCACAG GAGTCATGGA TTCTCGTGGC 
GAGCGTGCAG TGCTGGCTCT GGGAGGCAGT CTGGCCAGCT CAGTAAATGA GGCCTCCCAC 
TTAGTTACTG ATCGCATCCG CAGGACAGTC AAGTTCTTGT GTGCCCTGGG GAAGGGAATT 
CCCATCCTGT CCCTGAACTG GCTGTACCAG TCCCGAAAGG CTGGTTGCTT CTTGCCGCCT 
GATGACTACT TAGTGACTGA TCCTGAGCAA GAGAAGAACT TTAGCTTCAG CCTTCGGGAT 
TCCCTGTGCC GGGCTCGGGA ACGAAGACTG CTGGAGGACT ATGAGATTCA TGTGACCCCT 
GGCGTGCAGC CACCCCCCCC TCAGATGGGC GAGATCATCA GTTGCTGTGG AGGCACTTTC 
CTGCCCAGCA TGCCTCACTC CTATAAGCTT CACCGAGTCA TCATAACTTG CACTGAAGAC 
CTACCACGCT GTGCTATTCC ATCCCGCCTG GGGCTACCCC TCCTCTCTCC TGAGTTCCTC 
CTGACTGGAG TGCTGAAGCA GGAAGCCACA CCAGAGGCCT TTGTTCTCTC CAATTTGGAA 
ATGTCATCTA CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524326.1
CDS	156..4790
Translation	MESTQVIDWDAEEEEETELSSGSLGYSVEPIGQLRLFSGTHGPERDFPLYLGKNVVGRSPDCSVALPFPSISKQHAVIEISAWNKAPILQDCGSLNGTQIVKPPRVLPPGVSHRLRDQELILFADFPCQYHRLDVPPPLVPRSLLTIEKTPRIRIESQNSRVLLAADSEEEGDFPSGRCVANGQRNTASPSATVVPESDEEVSSPAPSVPGPSSPFGLGSDTDEEQGQQPGVEESSLADSSGAAGEAEQPEANGTTAGIQAQPTEHKLKDTKVKKEAGRAGVSDGSVLERSPTLGEDSDTEVDEDHKPGFADSETDVEEERIPVTPPVAPVKKNQVLLAVGIGDPEAPGVAHLQDCLAGSGTDVEDKTALDVPLERNHTPMVINSDTDEEEEEEEEEVSAALTLAHLKERGIGLWSRDPGAEEVKSQPQVLVEQSQSASGRDSDTDVEEESSGRKREIIPDSPMDVDEALTVTQPESQPPRRPNDADEYMDMSSPGSHLVVNQASFAVVGKTRAQVEEEVPGPSVILGEKHQVPLEGAQPPEEAWETAVQEGSSSPEAAASVRPSQQPVAEDAGTECATAVSEQESTLEVRSQSGSPAAPVEQVVIHTDTSGDPTLPQREGAQTPTGREREAHVGRTKSAKECCDAEPEDLCLPATQCFVEGESQHPEAVQSLENEPTQLFPCTLPQEPGPSHLSLQTPGADTLDVPWEVLATQPFCLREQSETSELHEAHGSQPSLPREPPGHQHLVHTSPVHTELLRIEGREIQTVEKAMGRLSCQTTPAGKASRGDPEPPDHCLFSSVPEASTQSLLTSQSQKQSTPQPLFSTSSSEIPLPESLHTKPNVRPRRSSRMTPSPHSSAALKPNTTCPTNQPAASRPTSRPTRGRANRSSTRTPELIVPVDPELQPSTSTEQPVIPKLTSQVTEGRVQMPEPLLTGPEIQSPTSTEQSVTPDRKPRATRGRPSKSPNKTPEPLISTGPELQPPTSIEQPVIPKPTSRVTRGRPRKSSVRTPESVVSTGPELQPLTSIEQPVIPEPRATRGRPSKSSIKTPESVVPTGPELQPLTSAKQPVTPNLTSRASRGRSSKSIRTPEPVVQTGPEFHPSTSTEQPDTREPSSQARTRRSAVKTPEASVPTTPELQPFTSKKQPAPKPTALVTQGRTYKPSTEDCESVGPVAPDFEPSTSTDHLVTPKVTDQSLTLQSSPLSASPVSSTPDLKPPVPIAQPVTPEPIPQANHQRKRRAAGKQGSRTVPLGHKSYSALSEPEPQSSASQSSGASEADSPRQKRPRRQASQKTVVIKEEPVETEVKEEPQETAIPTPEKRKRDHAEEVTQGKPTRSRRTKPNQETAPKVLFTGVMDSRGERAVLALGGSLASSVNEASHLVTDRIRRTVKFLCALGKGIPILSLNWLYQSRKAGCFLPPDDYLVTDPEQEKNFSFSLRDSLCRARERRLLEDYEIHVTPGVQPPPPQMGEIISCCGGTFLPSMPHSYKLHRVIITCTEDLPRCAIPSRLGLPLLSPEFLLTGVLKQEATPEAFVLSNLEMSST

Target ORF information:

RefSeq Version	XM_006524263.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus mediator of DNA damage checkpoint 1 (Mdc1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524263.4

ORF Insert Sequence:

ATGGAAAGCA CCCAGGTGAT TGACTGGGAT GCTGAAGAAG AGGAAGAGAC AGAACTATCC 
AGTGGCTCCT TGGGGTATAG TGTGGAGCCT ATAGGGCAGC TACGTCTCTT CAGTGGAACT 
CATGGACCAG AAAGAGATTT CCCCCTCTAC CTTGGCAAGA ATGTAGTTGG TCGAAGCCCT 
GACTGCTCTG TGGCCCTGCC TTTTCCATCC ATCTCCAAAC AGCATGCAGT AATTGAAATC 
TCAGCTTGGA ACAAAGCCCC TATCCTCCAG GATTGTGGGA GCCTCAATGG CACTCAAATC 
GTAAAGCCTC CTAGGGTCCT ACCTCCTGGA GTGAGCCATC GTCTGAGGGA CCAGGAATTA 
ATTCTGTTTG CAGATTTTCC CTGCCAGTAC CATCGCCTGG ATGTCCCACC ACCCTTGGTC 
CCTCGGAGTC TTCTAACTAT AGAGAAAACC CCCAGAATAC GGATAGAATC CCAAAATTCC 
AGAGTTTTGT TGGCTGCCGA TTCAGAGGAA GAAGGGGATT TTCCTTCTGG AAGGTGTGTG 
GCGAATGGAC AAAGGAATAC AGCATCCCCT TCAGCAACAG TAGTTCCAGA AAGTGATGAA 
GAGGTCTCTT CTCCGGCCCC AAGTGTCCCC GGGCCATCTT CACCCTTTGG TTTGGGCAGT 
GACACTGATG AAGAACAAGG TCAGCAACCA GGAGTAGAGG AGTCCTCTTT AGCTGACAGC 
AGTGGTGCTG CAGGGGAAGC TGAGCAGCCT GAAGCTAACG GAACGACAGC TGGCATACAG 
GCTCAGCCTA CTGAGCACAA ATTGAAGGAC ACAAAAGTCA AGAAGGAGGC AGGCAGGGCA 
GGGGTCTCAG ATGGGTCAGT TCTGGAGAGG AGCCCCACTC TTGGAGAGGA CAGTGACACA 
GAAGTGGACG AAGATCACAA ACCTGGGTTT GCGGACAGTG AGACAGATGT GGAAGAAGAG 
AGGATCCCTG TGACCCCCCC TGTAGCCCCT GTGAAGAAAA ACCAAGTGCT ACTTGCAGTT 
GGTATAGGAG ATCCTGAAGC ACCTGGTGTG GCACATCTGC AGGACTGCCT AGCTGGTAGT 
GGCACAGATG TGGAGGACAA GACTGCACTG GATGTCCCTC TAGAGAGAAA CCACACACCC 
ATGGTGATCA ACAGTGATAC AGATGAGGAG GAGGAGGAGG AGGAGGAGGA GGTCTCAGCA 
GCACTCACTT TGGCCCATCT GAAAGAGAGA GGAATTGGTT TGTGGAGTAG AGACCCAGGC 
GCAGAAGAGG TCAAGTCCCA ACCACAGGTC CTTGTAGAAC AAAGCCAGAG TGCCTCTGGG 
AGAGACAGTG ACACGGATGT GGAGGAGGAA TCCTCAGGGC GAAAGAGAGA GATTATCCCA 
GACAGTCCCA TGGATGTAGA TGAGGCTCTT ACTGTCACAC AGCCAGAGAG CCAGCCTCCC 
CGTAGGCCCA ATGATGCAGA TGAGTACATG GATATGAGCT CCCCTGGCAG CCATCTAGTG 
GTAAATCAGG CCTCCTTTGC TGTGGTAGGC AAGACCAGAG CACAAGTGGA GGAGGAAGTC 
CCAGGGCCAT CTGTTATCCT GGGGGAGAAG CACCAGGTGC CTCTAGAGGG AGCCCAGCCT 
CCTGAGGAAG CCTGGGAAAC AGCTGTTCAG GAAGGCTCAT CCTCACCAGA GGCAGCAGCC 
TCTGTAAGGC CTAGCCAGCA GCCAGTAGCA GAAGATGCTG GGACAGAGTG TGCTACAGCT 
GTTTCTGAGC AGGAAAGTAC TCTTGAGGTG AGGTCCCAAA GCGGGTCACC TGCAGCACCA 
GTGGAGCAAG TGGTGATACA TACAGATACT TCAGGGGATC CCACCCTGCC ACAGAGAGAG 
GGAGCCCAAA CCCCCACAGG AAGGGAGAGA GAAGCACATG TGGGCAGGAC CAAGAGTGCC 
AAAGAGTGCT GTGATGCAGA ACCTGAAGAT CTGTGCCTTC CAGCTACCCA GTGCTTTGTG 
GAAGGGGAGA GCCAGCACCC AGAAGCTGTC CAGAGTTTGG AAAATGAGCC TACCCAACTC 
TTCCCATGTA CTCTTCCACA AGAGCCAGGA CCTTCCCATC TCAGCCTTCA GACTCCAGGT 
GCAGATACCT TGGATGTGCC TTGGGAAGTC TTGGCTACAC AGCCGTTCTG TCTAAGAGAA 
CAGTCAGAGA CTTCTGAGCT TCATGAAGCT CATGGATCTC AACCATCTCT GCCTAGGGAA 
CCACCAGGAC ACCAACATCT GGTTCATACC AGCCCAGTTC ACACAGAGCT GTTGAGAATT 
GAAGGCAGAG AGATACAGAC TGTGGAGAAA GCCATGGGCC GCTTGAGTTG CCAGACGACA 
CCTGCTGGAA AGGCTTCCAG GGGTGATCCA GAGCCCCCAG ACCACTGCCT GTTTTCTTCA 
GTGCCTGAAG CTTCAACTCA GAGTCTCCTC ACCTCTCAGA GCCAAAAGCA GTCTACACCT 
CAGCCTCTGT TTTCGACCTC TTCTTCTGAG ATACCCCTTC CTGAGAGTCT TCACACAAAG 
CCTAATGTCA GGCCACGGCG GTCCTCCAGG ATGACCCCCT CCCCGCATTC CTCTGCTGCC 
CTTAAGCCAA ATACTACCTG CCCCACAAAC CAGCCTGCTG CCTCTAGACC AACATCCCGA 
CCCACTCGGG GCAGGGCAAA TAGGTCCTCT ACCAGGACCC CAGAACTGAT TGTCCCTGTA 
GACCCTGAAC TCCAGCCTTC CACCTCCACA GAACAGCCTG TCATCCCAAA ACTTACGTCC 
CAGGTCACTG AGGGCAGGGT ACAGATGCCT GAACCACTTC TCACAGGTCC CGAGATCCAG 
TCTCCCACCT CCACAGAACA GTCTGTCACC CCAGACCGCA AACCTCGGGC CACTCGGGGC 
AGGCCAAGTA AATCTCCCAA CAAGACCCCA GAACCACTTA TTTCTACTGG CCCTGAACTC 
CAACCTCCCA CCTCCATAGA ACAACCTGTC ATACCTAAAC CCACTTCTCG GGTCACTCGG 
GGCAGACCAC GTAAGTCTTC TGTCAGGACC CCAGAATCAG TTGTCTCCAC TGGCCCTGAA 
CTTCAGCCTC TCACCTCTAT AGAACAGCCT GTTATCCCTG AACCTAGGGC CACTCGAGGT 
AGACCAAGTA AGTCTTCTAT CAAGACCCCA GAATCAGTTG TCCCCACTGG TCCTGAGCTG 
CAGCCTCTTA CCTCTGCAAA ACAGCCTGTC ACCCCTAACC TCACATCTCG GGCCTCTCGG 
GGTAGATCAA GTAAGTCTAT CAGGACCCCG GAACCAGTTG TCCAAACTGG CCCTGAATTC 
CATCCATCCA CCTCCACAGA ACAGCCTGAT ACCCGTGAGC CCTCATCTCA GGCTAGGACA 
CGTAGGTCTG CTGTCAAGAC TCCTGAAGCG AGTGTCCCCA CAACCCCTGA ACTCCAGCCT 
TTCACTTCTA AAAAACAGCC TGCCCCTAAG CCTACAGCTC TGGTCACTCA GGGAAGGACA 
TATAAACCCT CCACTGAAGA TTGTGAATCA GTTGGGCCAG TGGCCCCTGA TTTTGAGCCT 
TCCACCTCTA CAGACCATCT TGTCACCCCT AAGGTTACAG ATCAGAGCTT AACACTACAG 
TCTTCACCCC TAAGTGCTTC CCCAGTTTCC AGTACCCCTG ATCTCAAGCC TCCTGTCCCC 
ATAGCCCAGC CTGTTACCCC AGAGCCCATT CCACAAGCCA ATCACCAAAG GAAACGAAGG 
GCTGCTGGGA AGCAGGGTTC CCGCACAGTC CCTCTGGGTC ATAAGTCTTA CTCTGCCCTC 
TCTGAACCTG AGCCCCAATC TTCAGCCAGC CAAAGCTCAG GAGCATCAGA AGCAGACTCA 
CCCCGTCAAA AACGTCCCAG AAGACAAGCC TCCCAGAAAA CTGTAGTCAT CAAGGAAGAA 
CCTGTAGAAA CAGAAGTGAA GGAAGAGCCT CAAGAGACTG CAATTCCAAC ACCAGAAAAG 
AGAAAGAGGG ACCATGCAGA AGAAGTGACC CAGGGAAAAC CAACTCGAAG CCGGCGAACT 
AAACCTAACC AAGAAACAGC CCCCAAGGTA CTGTTCACAG GAGTCATGGA TTCTCGTGGC 
GAGCGTGCAG TGCTGGCTCT GGGAGGCAGT CTGGCCAGCT CAGTAAATGA GGCCTCCCAC 
TTAGTTACTG ATCGCATCCG CAGGACAGTC AAGTTCTTGT GTGCCCTGGG GAAGGGAATT 
CCCATCCTGT CCCTGAACTG GCTGTACCAG TCCCGAAAGG CTGGTTGCTT CTTGCCGCCT 
GATGACTACT TAGTGACTGA TCCTGAGCAA GAGAAGAACT TTAGCTTCAG CCTTCGGGAT 
TCCCTGTGCC GGGCTCGGGA ACGAAGACTG CTGGAGGACT ATGAGATTCA TGTGACCCCT 
GGCGTGCAGC CACCCCCCCC TCAGATGGGC GAGATCATCA GTTGCTGTGG AGGCACTTTC 
CTGCCCAGCA TGCCTCACTC CTATAAGCTT CACCGAGTCA TCATAACTTG CACTGAAGAC 
CTACCACGCT GTGCTATTCC ATCCCGCCTG GGGCTACCCC TCCTCTCTCC TGAGTTCCTC 
CTGACTGGAG TGCTGAAGCA GGAAGCCACA CCAGAGGCCT TTGTTCTCTC CAATTTGGAA 
ATGTCATCTA CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mdc1 ( XM_006524264.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524327.1 Mus musculus mediator of DNA damage checkpoint 1 (Mdc1), transcript variant X6, mRNA.

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CloneID	OMu53580
Clone ID Related Accession (Same CDS sequence)	XM_006524264.4 , XM_006524264.3
Accession Version	XM_006524264.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4632bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	mediator of DNA damage checkpoint protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006524264.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGCA CCCAGGTGAT TGACTGGGAT GCTGAAGAAG AGGAAGAGAC AGAACTATCC 
AGTGGCTCCT TGGGGTATAG TGTGGAGCCT ATAGGGCAGC TACGTCTCTT CAGTGGAACT 
CATGGACCAG AAAGAGATTT CCCCCTCTAC CTTGGCAAGA ATGTAGTTGG TCGAAGCCCT 
GACTGCTCTG TGGCCCTGCC TTTTCCATCC ATCTCCAAAC AGCATGCAGT AATTGAAATC 
TCAGCTTGGA ACAAAGCCCC TATCCTCCAG GATTGTGGGA GCCTCAATGG CACTCAAATC 
GTAAAGCCTC CTAGGGTCCT ACCTCCTGGA GTGAGCCATC GTCTGAGGGA CCAGGAATTA 
ATTCTGTTTG CAGATTTTCC CTGCCAGTAC CATCGCCTGG ATGTCCCACC ACCCTTGGTC 
CCTCGGAGTC TTCTAACTAT AGAGAAAACC CCCAGAATAC GGATAGAATC CCAAAATTCC 
AGAGTTTTGT TGGCTGCCGA TTCAGAGGAA GAAGGGGATT TTCCTTCTGG AAGGTGTGTG 
GCGAATGGAC AAAGGAATAC AGCATCCCCT TCAGCAACAG TAGTTCCAGA AAGTGATGAA 
GAGGTCTCTT CTCCGGCCCC AAGTGTCCCC GGGCCATCTT CACCCTTTGG TTTGGGCAGT 
GACACTGATG AAGAACAAGG TCAGCAACCA GGAGTAGAGG AGTCCTCTTT AGCTGACAGC 
AGTGGTGCTG CAGGGGAAGC TGAGCAGCCT GAAGCTAACG GAACGACAGC TGGCATACAG 
GCTCAGCCTA CTGAGCACAA ATTGAAGGAC ACAAAAGTCA AGAAGGAGGC AGGCAGGGCA 
GGGGTCTCAG ATGGGTCAGT TCTGGAGAGG AGCCCCACTC TTGGAGAGGA CAGTGACACA 
GAAGTGGACG AAGATCACAA ACCTGGGTTT GCGGACAGTG AGACAGATGT GGAAGAAGAG 
AGGATCCCTG TGACCCCCCC TGTAGCCCCT GTGAAGAAAA ACCAAGTGCT ACTTGCAGTT 
GGTATAGGAG ATCCTGAAGC ACCTGGTGTG GCACATCTGC AGGACTGCCT AGCTGGTAGT 
GGCACAGATG TGGAGGACAA GACTGCACTG GATGTCCCTC TAGAGAGAAA CCACACACCC 
ATGGTGATCA ACAGTGATAC AGATGAGGAG GAGGAGGAGG AGGAGGAGGA GGTCTCAGCA 
GCACTCACTT TGGCCCATCT GAAAGAGAGA GGAATTGGTT TGTGGAGTAG AGACCCAGGC 
GCAGAAGAGG TCAAGTCCCA ACCACAGGTC CTTGTAGAAC AAAGCCAGAG TGCCTCTGGG 
AGAGACAGTG ACACGGATGT GGAGGAGGAA TCCTCAGGGC GAAAGAGAGA GATTATCCCA 
GACAGTCCCA TGGATGTAGA TGAGGCTCTT ACTGTCACAC AGCCAGAGAG CCAGCCTCCC 
CGTAGGCCCA ATGATGCAGA TGAGTACATG GATATGAGCT CCCCTGGCAG CCATCTAGTG 
GTAAATCAGG CCTCCTTTGC TGTGGTAGGC AAGACCAGAG CACAAGTGGA GGAGGAAGTC 
CCAGGGCCAT CTGTTATCCT GGGGGAGAAG CACCAGGTGC CTCTAGAGGG AGCCCAGCCT 
CCTGAGGAAG CCTGGGAAAC AGCTGTTCAG GAAGGCTCAT CCTCACCAGA GGCAGCAGCC 
TCTGTAAGGC CTAGCCAGCA GCCAGTAGCA GAAGATGCTG GGACAGAGTG TGCTACAGCT 
GTTTCTGAGC AGGAAAGTAC TCTTGAGGTG AGGTCCCAAA GCGGGTCACC TGCAGCACCA 
GTGGAGCAAG TGGTGATACA TACAGATACT TCAGGGGATC CCACCCTGCC ACAGAGAGAG 
GGAGCCCAAA CCCCCACAGG AAGGGAGAGA GAAGCACATG TGGGCAGGAC CAAGAGTGCC 
AAAGAGTGCT GTGATGAACC TGAAGATCTG TGCCTTCCAG CTACCCAGTG CTTTGTGGAA 
GGGGAGAGCC AGCACCCAGA AGCTGTCCAG AGTTTGGAAA ATGAGCCTAC CCAACTCTTC 
CCATGTACTC TTCCACAAGA GCCAGGACCT TCCCATCTCA GCCTTCAGAC TCCAGGTGCA 
GATACCTTGG ATGTGCCTTG GGAAGTCTTG GCTACACAGC CGTTCTGTCT AAGAGAACAG 
TCAGAGACTT CTGAGCTTCA TGAAGCTCAT GGATCTCAAC CATCTCTGCC TAGGGAACCA 
CCAGGACACC AACATCTGGT TCATACCAGC CCAGTTCACA CAGAGCTGTT GAGAATTGAA 
GGCAGAGAGA TACAGACTGT GGAGAAAGCC ATGGGCCGCT TGAGTTGCCA GACGACACCT 
GCTGGAAAGG CTTCCAGGGG TGATCCAGAG CCCCCAGACC ACTGCCTGTT TTCTTCAGTG 
CCTGAAGCTT CAACTCAGAG TCTCCTCACC TCTCAGAGCC AAAAGCAGTC TACACCTCAG 
CCTCTGTTTT CGACCTCTTC TTCTGAGATA CCCCTTCCTG AGAGTCTTCA CACAAAGCCT 
AATGTCAGGC CACGGCGGTC CTCCAGGATG ACCCCCTCCC CGCATTCCTC TGCTGCCCTT 
AAGCCAAATA CTACCTGCCC CACAAACCAG CCTGCTGCCT CTAGACCAAC ATCCCGACCC 
ACTCGGGGCA GGGCAAATAG GTCCTCTACC AGGACCCCAG AACTGATTGT CCCTGTAGAC 
CCTGAACTCC AGCCTTCCAC CTCCACAGAA CAGCCTGTCA TCCCAAAACT TACGTCCCAG 
GTCACTGAGG GCAGGGTACA GATGCCTGAA CCACTTCTCA CAGGTCCCGA GATCCAGTCT 
CCCACCTCCA CAGAACAGTC TGTCACCCCA GACCGCAAAC CTCGGGCCAC TCGGGGCAGG 
CCAAGTAAAT CTCCCAACAA GACCCCAGAA CCACTTATTT CTACTGGCCC TGAACTCCAA 
CCTCCCACCT CCATAGAACA ACCTGTCATA CCTAAACCCA CTTCTCGGGT CACTCGGGGC 
AGACCACGTA AGTCTTCTGT CAGGACCCCA GAATCAGTTG TCTCCACTGG CCCTGAACTT 
CAGCCTCTCA CCTCTATAGA ACAGCCTGTT ATCCCTGAAC CTAGGGCCAC TCGAGGTAGA 
CCAAGTAAGT CTTCTATCAA GACCCCAGAA TCAGTTGTCC CCACTGGTCC TGAGCTGCAG 
CCTCTTACCT CTGCAAAACA GCCTGTCACC CCTAACCTCA CATCTCGGGC CTCTCGGGGT 
AGATCAAGTA AGTCTATCAG GACCCCGGAA CCAGTTGTCC AAACTGGCCC TGAATTCCAT 
CCATCCACCT CCACAGAACA GCCTGATACC CGTGAGCCCT CATCTCAGGC TAGGACACGT 
AGGTCTGCTG TCAAGACTCC TGAAGCGAGT GTCCCCACAA CCCCTGAACT CCAGCCTTTC 
ACTTCTAAAA AACAGCCTGC CCCTAAGCCT ACAGCTCTGG TCACTCAGGG AAGGACATAT 
AAACCCTCCA CTGAAGATTG TGAATCAGTT GGGCCAGTGG CCCCTGATTT TGAGCCTTCC 
ACCTCTACAG ACCATCTTGT CACCCCTAAG GTTACAGATC AGAGCTTAAC ACTACAGTCT 
TCACCCCTAA GTGCTTCCCC AGTTTCCAGT ACCCCTGATC TCAAGCCTCC TGTCCCCATA 
GCCCAGCCTG TTACCCCAGA GCCCATTCCA CAAGCCAATC ACCAAAGGAA ACGAAGGGCT 
GCTGGGAAGC AGGGTTCCCG CACAGTCCCT CTGGGTCATA AGTCTTACTC TGCCCTCTCT 
GAACCTGAGC CCCAATCTTC AGCCAGCCAA AGCTCAGGAG CATCAGAAGC AGACTCACCC 
CGTCAAAAAC GTCCCAGAAG ACAAGCCTCC CAGAAAACTG TAGTCATCAA GGAAGAACCT 
GTAGAAACAG AAGTGAAGGA AGAGCCTCAA GAGACTGCAA TTCCAACACC AGAAAAGAGA 
AAGAGGGACC ATGCAGAAGA AGTGACCCAG GGAAAACCAA CTCGAAGCCG GCGAACTAAA 
CCTAACCAAG AAACAGCCCC CAAGGTACTG TTCACAGGAG TCATGGATTC TCGTGGCGAG 
CGTGCAGTGC TGGCTCTGGG AGGCAGTCTG GCCAGCTCAG TAAATGAGGC CTCCCACTTA 
GTTACTGATC GCATCCGCAG GACAGTCAAG TTCTTGTGTG CCCTGGGGAA GGGAATTCCC 
ATCCTGTCCC TGAACTGGCT GTACCAGTCC CGAAAGGCTG GTTGCTTCTT GCCGCCTGAT 
GACTACTTAG TGACTGATCC TGAGCAAGAG AAGAACTTTA GCTTCAGCCT TCGGGATTCC 
CTGTGCCGGG CTCGGGAACG AAGACTGCTG GAGGACTATG AGATTCATGT GACCCCTGGC 
GTGCAGCCAC CCCCCCCTCA GATGGGCGAG ATCATCAGTT GCTGTGGAGG CACTTTCCTG 
CCCAGCATGC CTCACTCCTA TAAGCTTCAC CGAGTCATCA TAACTTGCAC TGAAGACCTA 
CCACGCTGTG CTATTCCATC CCGCCTGGGG CTACCCCTCC TCTCTCCTGA GTTCCTCCTG 
ACTGGAGTGC TGAAGCAGGA AGCCACACCA GAGGCCTTTG TTCTCTCCAA TTTGGAAATG 
TCATCTACCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524327.1
CDS	155..4786
Translation	MESTQVIDWDAEEEEETELSSGSLGYSVEPIGQLRLFSGTHGPERDFPLYLGKNVVGRSPDCSVALPFPSISKQHAVIEISAWNKAPILQDCGSLNGTQIVKPPRVLPPGVSHRLRDQELILFADFPCQYHRLDVPPPLVPRSLLTIEKTPRIRIESQNSRVLLAADSEEEGDFPSGRCVANGQRNTASPSATVVPESDEEVSSPAPSVPGPSSPFGLGSDTDEEQGQQPGVEESSLADSSGAAGEAEQPEANGTTAGIQAQPTEHKLKDTKVKKEAGRAGVSDGSVLERSPTLGEDSDTEVDEDHKPGFADSETDVEEERIPVTPPVAPVKKNQVLLAVGIGDPEAPGVAHLQDCLAGSGTDVEDKTALDVPLERNHTPMVINSDTDEEEEEEEEEVSAALTLAHLKERGIGLWSRDPGAEEVKSQPQVLVEQSQSASGRDSDTDVEEESSGRKREIIPDSPMDVDEALTVTQPESQPPRRPNDADEYMDMSSPGSHLVVNQASFAVVGKTRAQVEEEVPGPSVILGEKHQVPLEGAQPPEEAWETAVQEGSSSPEAAASVRPSQQPVAEDAGTECATAVSEQESTLEVRSQSGSPAAPVEQVVIHTDTSGDPTLPQREGAQTPTGREREAHVGRTKSAKECCDEPEDLCLPATQCFVEGESQHPEAVQSLENEPTQLFPCTLPQEPGPSHLSLQTPGADTLDVPWEVLATQPFCLREQSETSELHEAHGSQPSLPREPPGHQHLVHTSPVHTELLRIEGREIQTVEKAMGRLSCQTTPAGKASRGDPEPPDHCLFSSVPEASTQSLLTSQSQKQSTPQPLFSTSSSEIPLPESLHTKPNVRPRRSSRMTPSPHSSAALKPNTTCPTNQPAASRPTSRPTRGRANRSSTRTPELIVPVDPELQPSTSTEQPVIPKLTSQVTEGRVQMPEPLLTGPEIQSPTSTEQSVTPDRKPRATRGRPSKSPNKTPEPLISTGPELQPPTSIEQPVIPKPTSRVTRGRPRKSSVRTPESVVSTGPELQPLTSIEQPVIPEPRATRGRPSKSSIKTPESVVPTGPELQPLTSAKQPVTPNLTSRASRGRSSKSIRTPEPVVQTGPEFHPSTSTEQPDTREPSSQARTRRSAVKTPEASVPTTPELQPFTSKKQPAPKPTALVTQGRTYKPSTEDCESVGPVAPDFEPSTSTDHLVTPKVTDQSLTLQSSPLSASPVSSTPDLKPPVPIAQPVTPEPIPQANHQRKRRAAGKQGSRTVPLGHKSYSALSEPEPQSSASQSSGASEADSPRQKRPRRQASQKTVVIKEEPVETEVKEEPQETAIPTPEKRKRDHAEEVTQGKPTRSRRTKPNQETAPKVLFTGVMDSRGERAVLALGGSLASSVNEASHLVTDRIRRTVKFLCALGKGIPILSLNWLYQSRKAGCFLPPDDYLVTDPEQEKNFSFSLRDSLCRARERRLLEDYEIHVTPGVQPPPPQMGEIISCCGGTFLPSMPHSYKLHRVIITCTEDLPRCAIPSRLGLPLLSPEFLLTGVLKQEATPEAFVLSNLEMSST

Target ORF information:

RefSeq Version	XM_006524264.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus mediator of DNA damage checkpoint 1 (Mdc1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524264.4

ORF Insert Sequence:

ATGGAAAGCA CCCAGGTGAT TGACTGGGAT GCTGAAGAAG AGGAAGAGAC AGAACTATCC 
AGTGGCTCCT TGGGGTATAG TGTGGAGCCT ATAGGGCAGC TACGTCTCTT CAGTGGAACT 
CATGGACCAG AAAGAGATTT CCCCCTCTAC CTTGGCAAGA ATGTAGTTGG TCGAAGCCCT 
GACTGCTCTG TGGCCCTGCC TTTTCCATCC ATCTCCAAAC AGCATGCAGT AATTGAAATC 
TCAGCTTGGA ACAAAGCCCC TATCCTCCAG GATTGTGGGA GCCTCAATGG CACTCAAATC 
GTAAAGCCTC CTAGGGTCCT ACCTCCTGGA GTGAGCCATC GTCTGAGGGA CCAGGAATTA 
ATTCTGTTTG CAGATTTTCC CTGCCAGTAC CATCGCCTGG ATGTCCCACC ACCCTTGGTC 
CCTCGGAGTC TTCTAACTAT AGAGAAAACC CCCAGAATAC GGATAGAATC CCAAAATTCC 
AGAGTTTTGT TGGCTGCCGA TTCAGAGGAA GAAGGGGATT TTCCTTCTGG AAGGTGTGTG 
GCGAATGGAC AAAGGAATAC AGCATCCCCT TCAGCAACAG TAGTTCCAGA AAGTGATGAA 
GAGGTCTCTT CTCCGGCCCC AAGTGTCCCC GGGCCATCTT CACCCTTTGG TTTGGGCAGT 
GACACTGATG AAGAACAAGG TCAGCAACCA GGAGTAGAGG AGTCCTCTTT AGCTGACAGC 
AGTGGTGCTG CAGGGGAAGC TGAGCAGCCT GAAGCTAACG GAACGACAGC TGGCATACAG 
GCTCAGCCTA CTGAGCACAA ATTGAAGGAC ACAAAAGTCA AGAAGGAGGC AGGCAGGGCA 
GGGGTCTCAG ATGGGTCAGT TCTGGAGAGG AGCCCCACTC TTGGAGAGGA CAGTGACACA 
GAAGTGGACG AAGATCACAA ACCTGGGTTT GCGGACAGTG AGACAGATGT GGAAGAAGAG 
AGGATCCCTG TGACCCCCCC TGTAGCCCCT GTGAAGAAAA ACCAAGTGCT ACTTGCAGTT 
GGTATAGGAG ATCCTGAAGC ACCTGGTGTG GCACATCTGC AGGACTGCCT AGCTGGTAGT 
GGCACAGATG TGGAGGACAA GACTGCACTG GATGTCCCTC TAGAGAGAAA CCACACACCC 
ATGGTGATCA ACAGTGATAC AGATGAGGAG GAGGAGGAGG AGGAGGAGGA GGTCTCAGCA 
GCACTCACTT TGGCCCATCT GAAAGAGAGA GGAATTGGTT TGTGGAGTAG AGACCCAGGC 
GCAGAAGAGG TCAAGTCCCA ACCACAGGTC CTTGTAGAAC AAAGCCAGAG TGCCTCTGGG 
AGAGACAGTG ACACGGATGT GGAGGAGGAA TCCTCAGGGC GAAAGAGAGA GATTATCCCA 
GACAGTCCCA TGGATGTAGA TGAGGCTCTT ACTGTCACAC AGCCAGAGAG CCAGCCTCCC 
CGTAGGCCCA ATGATGCAGA TGAGTACATG GATATGAGCT CCCCTGGCAG CCATCTAGTG 
GTAAATCAGG CCTCCTTTGC TGTGGTAGGC AAGACCAGAG CACAAGTGGA GGAGGAAGTC 
CCAGGGCCAT CTGTTATCCT GGGGGAGAAG CACCAGGTGC CTCTAGAGGG AGCCCAGCCT 
CCTGAGGAAG CCTGGGAAAC AGCTGTTCAG GAAGGCTCAT CCTCACCAGA GGCAGCAGCC 
TCTGTAAGGC CTAGCCAGCA GCCAGTAGCA GAAGATGCTG GGACAGAGTG TGCTACAGCT 
GTTTCTGAGC AGGAAAGTAC TCTTGAGGTG AGGTCCCAAA GCGGGTCACC TGCAGCACCA 
GTGGAGCAAG TGGTGATACA TACAGATACT TCAGGGGATC CCACCCTGCC ACAGAGAGAG 
GGAGCCCAAA CCCCCACAGG AAGGGAGAGA GAAGCACATG TGGGCAGGAC CAAGAGTGCC 
AAAGAGTGCT GTGATGAACC TGAAGATCTG TGCCTTCCAG CTACCCAGTG CTTTGTGGAA 
GGGGAGAGCC AGCACCCAGA AGCTGTCCAG AGTTTGGAAA ATGAGCCTAC CCAACTCTTC 
CCATGTACTC TTCCACAAGA GCCAGGACCT TCCCATCTCA GCCTTCAGAC TCCAGGTGCA 
GATACCTTGG ATGTGCCTTG GGAAGTCTTG GCTACACAGC CGTTCTGTCT AAGAGAACAG 
TCAGAGACTT CTGAGCTTCA TGAAGCTCAT GGATCTCAAC CATCTCTGCC TAGGGAACCA 
CCAGGACACC AACATCTGGT TCATACCAGC CCAGTTCACA CAGAGCTGTT GAGAATTGAA 
GGCAGAGAGA TACAGACTGT GGAGAAAGCC ATGGGCCGCT TGAGTTGCCA GACGACACCT 
GCTGGAAAGG CTTCCAGGGG TGATCCAGAG CCCCCAGACC ACTGCCTGTT TTCTTCAGTG 
CCTGAAGCTT CAACTCAGAG TCTCCTCACC TCTCAGAGCC AAAAGCAGTC TACACCTCAG 
CCTCTGTTTT CGACCTCTTC TTCTGAGATA CCCCTTCCTG AGAGTCTTCA CACAAAGCCT 
AATGTCAGGC CACGGCGGTC CTCCAGGATG ACCCCCTCCC CGCATTCCTC TGCTGCCCTT 
AAGCCAAATA CTACCTGCCC CACAAACCAG CCTGCTGCCT CTAGACCAAC ATCCCGACCC 
ACTCGGGGCA GGGCAAATAG GTCCTCTACC AGGACCCCAG AACTGATTGT CCCTGTAGAC 
CCTGAACTCC AGCCTTCCAC CTCCACAGAA CAGCCTGTCA TCCCAAAACT TACGTCCCAG 
GTCACTGAGG GCAGGGTACA GATGCCTGAA CCACTTCTCA CAGGTCCCGA GATCCAGTCT 
CCCACCTCCA CAGAACAGTC TGTCACCCCA GACCGCAAAC CTCGGGCCAC TCGGGGCAGG 
CCAAGTAAAT CTCCCAACAA GACCCCAGAA CCACTTATTT CTACTGGCCC TGAACTCCAA 
CCTCCCACCT CCATAGAACA ACCTGTCATA CCTAAACCCA CTTCTCGGGT CACTCGGGGC 
AGACCACGTA AGTCTTCTGT CAGGACCCCA GAATCAGTTG TCTCCACTGG CCCTGAACTT 
CAGCCTCTCA CCTCTATAGA ACAGCCTGTT ATCCCTGAAC CTAGGGCCAC TCGAGGTAGA 
CCAAGTAAGT CTTCTATCAA GACCCCAGAA TCAGTTGTCC CCACTGGTCC TGAGCTGCAG 
CCTCTTACCT CTGCAAAACA GCCTGTCACC CCTAACCTCA CATCTCGGGC CTCTCGGGGT 
AGATCAAGTA AGTCTATCAG GACCCCGGAA CCAGTTGTCC AAACTGGCCC TGAATTCCAT 
CCATCCACCT CCACAGAACA GCCTGATACC CGTGAGCCCT CATCTCAGGC TAGGACACGT 
AGGTCTGCTG TCAAGACTCC TGAAGCGAGT GTCCCCACAA CCCCTGAACT CCAGCCTTTC 
ACTTCTAAAA AACAGCCTGC CCCTAAGCCT ACAGCTCTGG TCACTCAGGG AAGGACATAT 
AAACCCTCCA CTGAAGATTG TGAATCAGTT GGGCCAGTGG CCCCTGATTT TGAGCCTTCC 
ACCTCTACAG ACCATCTTGT CACCCCTAAG GTTACAGATC AGAGCTTAAC ACTACAGTCT 
TCACCCCTAA GTGCTTCCCC AGTTTCCAGT ACCCCTGATC TCAAGCCTCC TGTCCCCATA 
GCCCAGCCTG TTACCCCAGA GCCCATTCCA CAAGCCAATC ACCAAAGGAA ACGAAGGGCT 
GCTGGGAAGC AGGGTTCCCG CACAGTCCCT CTGGGTCATA AGTCTTACTC TGCCCTCTCT 
GAACCTGAGC CCCAATCTTC AGCCAGCCAA AGCTCAGGAG CATCAGAAGC AGACTCACCC 
CGTCAAAAAC GTCCCAGAAG ACAAGCCTCC CAGAAAACTG TAGTCATCAA GGAAGAACCT 
GTAGAAACAG AAGTGAAGGA AGAGCCTCAA GAGACTGCAA TTCCAACACC AGAAAAGAGA 
AAGAGGGACC ATGCAGAAGA AGTGACCCAG GGAAAACCAA CTCGAAGCCG GCGAACTAAA 
CCTAACCAAG AAACAGCCCC CAAGGTACTG TTCACAGGAG TCATGGATTC TCGTGGCGAG 
CGTGCAGTGC TGGCTCTGGG AGGCAGTCTG GCCAGCTCAG TAAATGAGGC CTCCCACTTA 
GTTACTGATC GCATCCGCAG GACAGTCAAG TTCTTGTGTG CCCTGGGGAA GGGAATTCCC 
ATCCTGTCCC TGAACTGGCT GTACCAGTCC CGAAAGGCTG GTTGCTTCTT GCCGCCTGAT 
GACTACTTAG TGACTGATCC TGAGCAAGAG AAGAACTTTA GCTTCAGCCT TCGGGATTCC 
CTGTGCCGGG CTCGGGAACG AAGACTGCTG GAGGACTATG AGATTCATGT GACCCCTGGC 
GTGCAGCCAC CCCCCCCTCA GATGGGCGAG ATCATCAGTT GCTGTGGAGG CACTTTCCTG 
CCCAGCATGC CTCACTCCTA TAAGCTTCAC CGAGTCATCA TAACTTGCAC TGAAGACCTA 
CCACGCTGTG CTATTCCATC CCGCCTGGGG CTACCCCTCC TCTCTCCTGA GTTCCTCCTG 
ACTGGAGTGC TGAAGCAGGA AGCCACACCA GAGGCCTTTG TTCTCTCCAA TTTGGAAATG 
TCATCTACCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mdc1 ( NM_001010833.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001010833.2 Mus musculus mediator of DNA damage checkpoint 1 (Mdc1), mRNA.

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CloneID	OMu19129
Clone ID Related Accession (Same CDS sequence)	NM_001010833.2 , XM_006524260.1 , XM_006524260.2 , XM_006524261.3
Accession Version	NM_001010833.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	mediator of DNA damage checkpoint protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CR974451.9, BY022698.1, AK033009.1, BC094363.1 and BC085140.1. On Mar 15, 2007 this sequence version replaced NM_001010833.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGAAAGCA CCCAGGTGAT TGACTGGGAT GCTGAAGAAG AGGAAGAGAC AGAACTATCC 
AGTGGCTCCT TGGGGTATAG TGTGGAGCCT ATAGGGCAGC TACGTCTCTT CAGTGGAACT 
CATGGACCAG AAAGAGATTT CCCCCTCTAC CTTGGCAAGA ATGTAGTTGG TCGAAGCCCT 
GACTGCTCTG TGGCCCTGCC TTTTCCATCC ATCTCCAAAC AGCATGCAGT AATTGAAATC 
TCAGCTTGGA ACAAAGCCCC TATCCTCCAG GATTGTGGGA GCCTCAATGG CACTCAAATC 
GTAAAGCCTC CTAGGGTCCT ACCTCCTGGA GTGAGCCATC GTCTGAGGGA CCAGGAATTA 
ATTCTGTTTG CAGATTTTCC CTGCCAGTAC CATCGCCTGG ATGTCCCACC ACCCTTGGTC 
CCTCGGAGTC TTCTAACTAT AGAGAAAACC CCCAGAATAC GGATAGAATC CCAAAATTCC 
AGAGTTTTGT TGGCTGCCGA TTCAGAGGAA GAAGGGGATT TTCCTTCTGG AAGGTGTGTG 
GCGAATGGAC AAAGGAATAC AGCATCCCCT TCAGCAACAG TAGTTCCAGA AAGTGATGAA 
GAGGTCTCTT CTCCGGCCCC AAGTGTCCCC GGGCCATCTT CACCCTTTGG TTTGGGCAGT 
GACACTGATG AAGAACAAGG TCAGCAACCA GGAGTAGAGG AGTCCTCTTT AGCTGACAGC 
AGTGGTGCTG CAGGGGAAGC TGAGCAGCCT GAAGCTAACG GAACGACAGC TGGCATACAG 
GCTCAGCCTA CTGAGCACAA ATTGAAGGAC ACAAAAGTCA AGAAGGAGGC AGGCAGGGCA 
GGGGTCTCAG ATGGGTCAGT TCTGGAGAGG AGCCCCACTC TTGGAGAGGA CAGTGACACA 
GAAGTGGACG AAGATCACAA ACCTGGGTTT GCGGACAGTG AGACAGATGT GGAAGAAGAG 
AGGATCCCTG TGACCCCCCC TGTAGCCCCT GTGAAGAAAA ACCAAGTGCT ACTTGCAGTT 
GGTATAGGAG ATCCTGAAGC ACCTGGTGTG GCACATCTGC AGGACTGCCT AGCTGGTAGT 
GGCACAGATG TGGAGGACAA GACTGCACTG GATGTCCCTC TAGAGAGAAA CCACACACCC 
ATGGTGATCA ACAGTGATAC AGATGAGGAG GAGGAGGAGG AGGAGGAGGA GGTCTCAGCA 
GCACTCACTT TGGCCCATCT GAAAGAGAGA GGAATTGGTT TGTGGAGTAG AGACCCAGGC 
GCAGAAGAGG TCAAGTCCCA ACCACAGGTC CTTGTAGAAC AAAGCCAGAG TGCCTCTGGG 
AGAGACAGTG ACACGGATGT GGAGGAGGAA TCCTCAGGGC GAAAGAGAGA GATTATCCCA 
GACAGTCCCA TGGATGTAGA TGAGGCTCTT ACTGTCACAC AGCCAGAGAG CCAGCCTCCC 
CGTAGGCCCA ATGATGCAGA TGAGTACATG GATATGAGCT CCCCTGGCAG CCATCTAGTG 
GTAAATCAGG CCTCCTTTGC TGTGGTAGGC AAGACCAGAG CACAAGTGGA GGAGGAAGTC 
CCAGGGCCAT CTGTTATCCT GGGGGAGAAG CACCAGGTGC CTCTAGAGGG AGCCCAGCCT 
CCTGAGGAAG CCTGGGAAAC AGCTGTTCAG GAAGGCTCAT CCTCACCAGA GGCAGCAGCC 
TCTGTAAGGC CTAGCCAGCA GCCAGTAGCA GAAGATGCTG GGACAGAGTG TGCTACAGCT 
GTTTCTGAGC AGGAAAGTAC TCTTGAGGTG AGGTCCCAAA GCGGGTCACC TGCAGCACCA 
GTGGAGCAAG TGGTGATACA TACAGATACT TCAGGGGATC CCACCCTGCC ACAGAGAGAG 
GGAGCCCAAA CCCCCACAGG AAGGGAGAGA GAAGCACATG TGGGCAGGAC CAAGAGTGCC 
AAAGAGTGCT GTGATGCAGA ACCTGAAGAT CTGTGCCTTC CAGCTACCCA GTGCTTTGTG 
GAAGGGGAGA GCCAGCACCC AGAAGCTGTC CAGAGTTTGG AAAATGAGCC TACCCAACTC 
TTCCCATGTA CTCTTCCACA AGAGCCAGGA CCTTCCCATC TCAGCCTTCA GACTCCAGGT 
GCAGATACCT TGGATGTGCC TTGGGAAGTC TTGGCTACAC AGCCGTTCTG TCTAAGAGAA 
CAGTCAGAGA CTTCTGAGCT TCATGAAGCT CATGGATCTC AACCATCTCT GCCTAGGGAA 
CCACCAGGAC ACCAACATCT GGTTCATACC AGCCCAGTTC ACACAGAGCT GTTGAGAATT 
GAAGGCAGAG AGATACAGAC TGTGGAGAAA GCCATGGGTA TACCAAAGGA AATGGCAGAC 
AGGATGACCC CTGAGAGAGA GCCATTGGAG AGGGAAATCA GGGGAAGAAC AGAAAACAGT 
GAGAGAGATG TGATAGGAGA AGAACTAATT CAGGGGACAA AGGACAGAGA GCCAAAGAAG 
GTGTTAGCTA GAGACAGTCA GAGAAAAGAG GCTGACAAGG ATCTTGAAGG GAATAGGGAG 
AGTTTGGAGG TAGAAATAGA GATGTCCAAA GACTCACAGA AGAGAGAGAG AAAAGTAGAG 
AAACCAGAAC CAAAGAGAGA ATGGGAGCCA GCTGATTTAG AAGTGACTCC AGATAGAGGG 
GTGACAGAGG AAGGGAGCCA TGATCAGAAA GGGCAAATTG CTAGTCTAAC ACTAAAGCCT 
GGAGTTGGGG TGAAGGACCT TGAGGGACTT GCATCAGCCC CAATAATCAC AGGGAGCCAG 
GCAGATGGAG GAAAGGGAGA CCCTTTGAGC CCTGGGAGGC AGCAGAGAGG CCGCTTGAGT 
TGCCAGACGA CACCTGCTGG AAAGGCTTCC AGGGGTGATC CAGAGCCCCC AGACCACTGC 
CTGTTTTCTT CAGTGCCTGA AGCTTCAACT CAGAGTCTCC TCACCTCTCA GAGCCAAAAG 
CAGTCTACAC CTCAGCCTCT GTTTTCGACC TCTTCTTCTG AGATACCCCT TCCTGAGAGT 
CTTCACACAA AGCCTAATGT CAGGCCACGG CGGTCCTCCA GGATGACCCC CTCCCCGCAT 
TCCTCTGCTG CCCTTAAGCC AAATACTACC TGCCCCACAA ACCAGCCTGC TGCCTCTAGA 
CCAACATCCC GACCCACTCG GGGCAGGGCA AATAGGTCCT CTACCAGGAC CCCAGAACTG 
ATTGTCCCTG TAGACCCTGA ACTCCAGCCT TCCACCTCCA CAGAACAGCC TGTCATCCCA 
AAACTTACGT CCCAGGTCAC TGAGGGCAGG GTACAGATGC CTGAACCACT TCTCACAGGT 
CCCGAGATCC AGTCTCCCAC CTCCACAGAA CAGTCTGTCA CCCCAGACCG CAAACCTCGG 
GCCACTCGGG GCAGGCCAAG TAAATCTCCC AACAAGACCC CAGAACCACT TATTTCTACT 
GGCCCTGAAC TCCAACCTCC CACCTCCATA GAACAACCTG TCATACCTAA ACCCACTTCT 
CGGGTCACTC GGGGCAGACC ACGTAAGTCT TCTGTCAGGA CCCCAGAATC AGTTGTCTCC 
ACTGGCCCTG AACTTCAGCC TCTCACCTCT ATAGAACAGC CTGTTATCCC TGAACCTAGG 
GCCACTCGAG GTAGACCAAG TAAGTCTTCT ATCAAGACCC CAGAATCAGT TGTCCCCACT 
GGTCCTGAGC TGCAGCCTCT TACCTCTGCA AAACAGCCTG TCACCCCTAA CCTCACATCT 
CGGGCCTCTC GGGGTAGATC AAGTAAGTCT ATCAGGACCC CGGAACCAGT TGTCCAAACT 
GGCCCTGAAT TCCATCCATC CACCTCCACA GAACAGCCTG ATACCCGTGA GCCCTCATCT 
CAGGCTAGGA CACGTAGGTC TGCTGTCAAG ACTCCTGAAG CGAGTGTCCC CACAACCCCT 
GAACTCCAGC CTTTCACTTC TAAAAAACAG CCTGCCCCTA AGCCTACAGC TCTGGTCACT 
CAGGGAAGGA CATATAAACC CTCCACTGAA GATTGTGAAT CAGTTGGGCC AGTGGCCCCT 
GATTTTGAGC CTTCCACCTC TACAGACCAT CTTGTCACCC CTAAGGTTAC AGATCAGAGC 
TTAACACTAC AGTCTTCACC CCTAAGTGCT TCCCCAGTTT CCAGTACCCC TGATCTCAAG 
CCTCCTGTCC CCATAGCCCA GCCTGTTACC CCAGAGCCCA TTCCACAAGC CAATCACCAA 
AGGAAACGAA GGGCTGCTGG GAAGCAGGGT TCCCGCACAG TCCCTCTGGG TCATAAGTCT 
TACTCTGCCC TCTCTGAACC TGAGCCCCAA TCTTCAGCCA GCCAAAGCTC AGGAGCATCA 
GAAGCAGACT CACCCCGTCA AAAACGTCCC AGAAGACAAG CCTCCCAGAA AACTGTAGTC 
ATCAAGGAAG AACCTGTAGA AACAGAAGTG AAGGAAGAGC CTCAAGAGAC TGCAATTCCA 
ACACCAGAAA AGAGAAAGAG GGACCATGCA GAAGAAGTGA CCCAGGGAAA ACCAACTCGA 
AGCCGGCGAA CTAAACCTAA CCAAGAAACA GCCCCCAAGG TACTGTTCAC AGGAGTCATG 
GATTCTCGTG GCGAGCGTGC AGTGCTGGCT CTGGGAGGCA GTCTGGCCAG CTCAGTAAAT 
GAGGCCTCCC ACTTAGTTAC TGATCGCATC CGCAGGACAG TCAAGTTCTT GTGTGCCCTG 
GGGAAGGGAA TTCCCATCCT GTCCCTGAAC TGGCTGTACC AGTCCCGAAA GGCTGGTTGC 
TTCTTGCCGC CTGATGACTA CTTAGTGACT GATCCTGAGC AAGAGAAGAA CTTTAGCTTC 
AGCCTTCGGG ATTCCCTGTG CCGGGCTCGG GAACGAAGAC TGCTGGAGGA CTATGAGATT 
CATGTGACCC CTGGCGTGCA GCCACCCCCC CCTCAGATGG GCGAGATCAT CAGTTGCTGT 
GGAGGCACTT TCCTGCCCAG CATGCCTCAC TCCTATAAGC TTCACCGAGT CATCATAACT 
TGCACTGAAG ACCTACCACG CTGTGCTATT CCATCCCGCC TGGGGCTACC CCTCCTCTCT 
CCTGAGTTCC TCCTGACTGG AGTGCTGAAG CAGGAAGCCA CACCAGAGGC CTTTGTTCTC 
TCCAATTTGG AAATGTCATC TACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001010833.2
CDS	186..5312
Translation	MESTQVIDWDAEEEEETELSSGSLGYSVEPIGQLRLFSGTHGPERDFPLYLGKNVVGRSPDCSVALPFPSISKQHAVIEISAWNKAPILQDCGSLNGTQIVKPPRVLPPGVSHRLRDQELILFADFPCQYHRLDVPPPLVPRSLLTIEKTPRIRIESQNSRVLLAADSEEEGDFPSGRCVANGQRNTASPSATVVPESDEEVSSPAPSVPGPSSPFGLGSDTDEEQGQQPGVEESSLADSSGAAGEAEQPEANGTTAGIQAQPTEHKLKDTKVKKEAGRAGVSDGSVLERSPTLGEDSDTEVDEDHKPGFADSETDVEEERIPVTPPVAPVKKNQVLLAVGIGDPEAPGVAHLQDCLAGSGTDVEDKTALDVPLERNHTPMVINSDTDEEEEEEEEEVSAALTLAHLKERGIGLWSRDPGAEEVKSQPQVLVEQSQSASGRDSDTDVEEESSGRKREIIPDSPMDVDEALTVTQPESQPPRRPNDADEYMDMSSPGSHLVVNQASFAVVGKTRAQVEEEVPGPSVILGEKHQVPLEGAQPPEEAWETAVQEGSSSPEAAASVRPSQQPVAEDAGTECATAVSEQESTLEVRSQSGSPAAPVEQVVIHTDTSGDPTLPQREGAQTPTGREREAHVGRTKSAKECCDAEPEDLCLPATQCFVEGESQHPEAVQSLENEPTQLFPCTLPQEPGPSHLSLQTPGADTLDVPWEVLATQPFCLREQSETSELHEAHGSQPSLPREPPGHQHLVHTSPVHTELLRIEGREIQTVEKAMGIPKEMADRMTPEREPLEREIRGRTENSERDVIGEELIQGTKDREPKKVLARDSQRKEADKDLEGNRESLEVEIEMSKDSQKRERKVEKPEPKREWEPADLEVTPDRGVTEEGSHDQKGQIASLTLKPGVGVKDLEGLASAPIITGSQADGGKGDPLSPGRQQRGRLSCQTTPAGKASRGDPEPPDHCLFSSVPEASTQSLLTSQSQKQSTPQPLFSTSSSEIPLPESLHTKPNVRPRRSSRMTPSPHSSAALKPNTTCPTNQPAASRPTSRPTRGRANRSSTRTPELIVPVDPELQPSTSTEQPVIPKLTSQVTEGRVQMPEPLLTGPEIQSPTSTEQSVTPDRKPRATRGRPSKSPNKTPEPLISTGPELQPPTSIEQPVIPKPTSRVTRGRPRKSSVRTPESVVSTGPELQPLTSIEQPVIPEPRATRGRPSKSSIKTPESVVPTGPELQPLTSAKQPVTPNLTSRASRGRSSKSIRTPEPVVQTGPEFHPSTSTEQPDTREPSSQARTRRSAVKTPEASVPTTPELQPFTSKKQPAPKPTALVTQGRTYKPSTEDCESVGPVAPDFEPSTSTDHLVTPKVTDQSLTLQSSPLSASPVSSTPDLKPPVPIAQPVTPEPIPQANHQRKRRAAGKQGSRTVPLGHKSYSALSEPEPQSSASQSSGASEADSPRQKRPRRQASQKTVVIKEEPVETEVKEEPQETAIPTPEKRKRDHAEEVTQGKPTRSRRTKPNQETAPKVLFTGVMDSRGERAVLALGGSLASSVNEASHLVTDRIRRTVKFLCALGKGIPILSLNWLYQSRKAGCFLPPDDYLVTDPEQEKNFSFSLRDSLCRARERRLLEDYEIHVTPGVQPPPPQMGEIISCCGGTFLPSMPHSYKLHRVIITCTEDLPRCAIPSRLGLPLLSPEFLLTGVLKQEATPEAFVLSNLEMSST

Target ORF information:

RefSeq Version	NM_001010833.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus mediator of DNA damage checkpoint 1 (Mdc1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001010833.2

ORF Insert Sequence:

ATGGAAAGCA CCCAGGTGAT TGACTGGGAT GCTGAAGAAG AGGAAGAGAC AGAACTATCC 
AGTGGCTCCT TGGGGTATAG TGTGGAGCCT ATAGGGCAGC TACGTCTCTT CAGTGGAACT 
CATGGACCAG AAAGAGATTT CCCCCTCTAC CTTGGCAAGA ATGTAGTTGG TCGAAGCCCT 
GACTGCTCTG TGGCCCTGCC TTTTCCATCC ATCTCCAAAC AGCATGCAGT AATTGAAATC 
TCAGCTTGGA ACAAAGCCCC TATCCTCCAG GATTGTGGGA GCCTCAATGG CACTCAAATC 
GTAAAGCCTC CTAGGGTCCT ACCTCCTGGA GTGAGCCATC GTCTGAGGGA CCAGGAATTA 
ATTCTGTTTG CAGATTTTCC CTGCCAGTAC CATCGCCTGG ATGTCCCACC ACCCTTGGTC 
CCTCGGAGTC TTCTAACTAT AGAGAAAACC CCCAGAATAC GGATAGAATC CCAAAATTCC 
AGAGTTTTGT TGGCTGCCGA TTCAGAGGAA GAAGGGGATT TTCCTTCTGG AAGGTGTGTG 
GCGAATGGAC AAAGGAATAC AGCATCCCCT TCAGCAACAG TAGTTCCAGA AAGTGATGAA 
GAGGTCTCTT CTCCGGCCCC AAGTGTCCCC GGGCCATCTT CACCCTTTGG TTTGGGCAGT 
GACACTGATG AAGAACAAGG TCAGCAACCA GGAGTAGAGG AGTCCTCTTT AGCTGACAGC 
AGTGGTGCTG CAGGGGAAGC TGAGCAGCCT GAAGCTAACG GAACGACAGC TGGCATACAG 
GCTCAGCCTA CTGAGCACAA ATTGAAGGAC ACAAAAGTCA AGAAGGAGGC AGGCAGGGCA 
GGGGTCTCAG ATGGGTCAGT TCTGGAGAGG AGCCCCACTC TTGGAGAGGA CAGTGACACA 
GAAGTGGACG AAGATCACAA ACCTGGGTTT GCGGACAGTG AGACAGATGT GGAAGAAGAG 
AGGATCCCTG TGACCCCCCC TGTAGCCCCT GTGAAGAAAA ACCAAGTGCT ACTTGCAGTT 
GGTATAGGAG ATCCTGAAGC ACCTGGTGTG GCACATCTGC AGGACTGCCT AGCTGGTAGT 
GGCACAGATG TGGAGGACAA GACTGCACTG GATGTCCCTC TAGAGAGAAA CCACACACCC 
ATGGTGATCA ACAGTGATAC AGATGAGGAG GAGGAGGAGG AGGAGGAGGA GGTCTCAGCA 
GCACTCACTT TGGCCCATCT GAAAGAGAGA GGAATTGGTT TGTGGAGTAG AGACCCAGGC 
GCAGAAGAGG TCAAGTCCCA ACCACAGGTC CTTGTAGAAC AAAGCCAGAG TGCCTCTGGG 
AGAGACAGTG ACACGGATGT GGAGGAGGAA TCCTCAGGGC GAAAGAGAGA GATTATCCCA 
GACAGTCCCA TGGATGTAGA TGAGGCTCTT ACTGTCACAC AGCCAGAGAG CCAGCCTCCC 
CGTAGGCCCA ATGATGCAGA TGAGTACATG GATATGAGCT CCCCTGGCAG CCATCTAGTG 
GTAAATCAGG CCTCCTTTGC TGTGGTAGGC AAGACCAGAG CACAAGTGGA GGAGGAAGTC 
CCAGGGCCAT CTGTTATCCT GGGGGAGAAG CACCAGGTGC CTCTAGAGGG AGCCCAGCCT 
CCTGAGGAAG CCTGGGAAAC AGCTGTTCAG GAAGGCTCAT CCTCACCAGA GGCAGCAGCC 
TCTGTAAGGC CTAGCCAGCA GCCAGTAGCA GAAGATGCTG GGACAGAGTG TGCTACAGCT 
GTTTCTGAGC AGGAAAGTAC TCTTGAGGTG AGGTCCCAAA GCGGGTCACC TGCAGCACCA 
GTGGAGCAAG TGGTGATACA TACAGATACT TCAGGGGATC CCACCCTGCC ACAGAGAGAG 
GGAGCCCAAA CCCCCACAGG AAGGGAGAGA GAAGCACATG TGGGCAGGAC CAAGAGTGCC 
AAAGAGTGCT GTGATGCAGA ACCTGAAGAT CTGTGCCTTC CAGCTACCCA GTGCTTTGTG 
GAAGGGGAGA GCCAGCACCC AGAAGCTGTC CAGAGTTTGG AAAATGAGCC TACCCAACTC 
TTCCCATGTA CTCTTCCACA AGAGCCAGGA CCTTCCCATC TCAGCCTTCA GACTCCAGGT 
GCAGATACCT TGGATGTGCC TTGGGAAGTC TTGGCTACAC AGCCGTTCTG TCTAAGAGAA 
CAGTCAGAGA CTTCTGAGCT TCATGAAGCT CATGGATCTC AACCATCTCT GCCTAGGGAA 
CCACCAGGAC ACCAACATCT GGTTCATACC AGCCCAGTTC ACACAGAGCT GTTGAGAATT 
GAAGGCAGAG AGATACAGAC TGTGGAGAAA GCCATGGGTA TACCAAAGGA AATGGCAGAC 
AGGATGACCC CTGAGAGAGA GCCATTGGAG AGGGAAATCA GGGGAAGAAC AGAAAACAGT 
GAGAGAGATG TGATAGGAGA AGAACTAATT CAGGGGACAA AGGACAGAGA GCCAAAGAAG 
GTGTTAGCTA GAGACAGTCA GAGAAAAGAG GCTGACAAGG ATCTTGAAGG GAATAGGGAG 
AGTTTGGAGG TAGAAATAGA GATGTCCAAA GACTCACAGA AGAGAGAGAG AAAAGTAGAG 
AAACCAGAAC CAAAGAGAGA ATGGGAGCCA GCTGATTTAG AAGTGACTCC AGATAGAGGG 
GTGACAGAGG AAGGGAGCCA TGATCAGAAA GGGCAAATTG CTAGTCTAAC ACTAAAGCCT 
GGAGTTGGGG TGAAGGACCT TGAGGGACTT GCATCAGCCC CAATAATCAC AGGGAGCCAG 
GCAGATGGAG GAAAGGGAGA CCCTTTGAGC CCTGGGAGGC AGCAGAGAGG CCGCTTGAGT 
TGCCAGACGA CACCTGCTGG AAAGGCTTCC AGGGGTGATC CAGAGCCCCC AGACCACTGC 
CTGTTTTCTT CAGTGCCTGA AGCTTCAACT CAGAGTCTCC TCACCTCTCA GAGCCAAAAG 
CAGTCTACAC CTCAGCCTCT GTTTTCGACC TCTTCTTCTG AGATACCCCT TCCTGAGAGT 
CTTCACACAA AGCCTAATGT CAGGCCACGG CGGTCCTCCA GGATGACCCC CTCCCCGCAT 
TCCTCTGCTG CCCTTAAGCC AAATACTACC TGCCCCACAA ACCAGCCTGC TGCCTCTAGA 
CCAACATCCC GACCCACTCG GGGCAGGGCA AATAGGTCCT CTACCAGGAC CCCAGAACTG 
ATTGTCCCTG TAGACCCTGA ACTCCAGCCT TCCACCTCCA CAGAACAGCC TGTCATCCCA 
AAACTTACGT CCCAGGTCAC TGAGGGCAGG GTACAGATGC CTGAACCACT TCTCACAGGT 
CCCGAGATCC AGTCTCCCAC CTCCACAGAA CAGTCTGTCA CCCCAGACCG CAAACCTCGG 
GCCACTCGGG GCAGGCCAAG TAAATCTCCC AACAAGACCC CAGAACCACT TATTTCTACT 
GGCCCTGAAC TCCAACCTCC CACCTCCATA GAACAACCTG TCATACCTAA ACCCACTTCT 
CGGGTCACTC GGGGCAGACC ACGTAAGTCT TCTGTCAGGA CCCCAGAATC AGTTGTCTCC 
ACTGGCCCTG AACTTCAGCC TCTCACCTCT ATAGAACAGC CTGTTATCCC TGAACCTAGG 
GCCACTCGAG GTAGACCAAG TAAGTCTTCT ATCAAGACCC CAGAATCAGT TGTCCCCACT 
GGTCCTGAGC TGCAGCCTCT TACCTCTGCA AAACAGCCTG TCACCCCTAA CCTCACATCT 
CGGGCCTCTC GGGGTAGATC AAGTAAGTCT ATCAGGACCC CGGAACCAGT TGTCCAAACT 
GGCCCTGAAT TCCATCCATC CACCTCCACA GAACAGCCTG ATACCCGTGA GCCCTCATCT 
CAGGCTAGGA CACGTAGGTC TGCTGTCAAG ACTCCTGAAG CGAGTGTCCC CACAACCCCT 
GAACTCCAGC CTTTCACTTC TAAAAAACAG CCTGCCCCTA AGCCTACAGC TCTGGTCACT 
CAGGGAAGGA CATATAAACC CTCCACTGAA GATTGTGAAT CAGTTGGGCC AGTGGCCCCT 
GATTTTGAGC CTTCCACCTC TACAGACCAT CTTGTCACCC CTAAGGTTAC AGATCAGAGC 
TTAACACTAC AGTCTTCACC CCTAAGTGCT TCCCCAGTTT CCAGTACCCC TGATCTCAAG 
CCTCCTGTCC CCATAGCCCA GCCTGTTACC CCAGAGCCCA TTCCACAAGC CAATCACCAA 
AGGAAACGAA GGGCTGCTGG GAAGCAGGGT TCCCGCACAG TCCCTCTGGG TCATAAGTCT 
TACTCTGCCC TCTCTGAACC TGAGCCCCAA TCTTCAGCCA GCCAAAGCTC AGGAGCATCA 
GAAGCAGACT CACCCCGTCA AAAACGTCCC AGAAGACAAG CCTCCCAGAA AACTGTAGTC 
ATCAAGGAAG AACCTGTAGA AACAGAAGTG AAGGAAGAGC CTCAAGAGAC TGCAATTCCA 
ACACCAGAAA AGAGAAAGAG GGACCATGCA GAAGAAGTGA CCCAGGGAAA ACCAACTCGA 
AGCCGGCGAA CTAAACCTAA CCAAGAAACA GCCCCCAAGG TACTGTTCAC AGGAGTCATG 
GATTCTCGTG GCGAGCGTGC AGTGCTGGCT CTGGGAGGCA GTCTGGCCAG CTCAGTAAAT 
GAGGCCTCCC ACTTAGTTAC TGATCGCATC CGCAGGACAG TCAAGTTCTT GTGTGCCCTG 
GGGAAGGGAA TTCCCATCCT GTCCCTGAAC TGGCTGTACC AGTCCCGAAA GGCTGGTTGC 
TTCTTGCCGC CTGATGACTA CTTAGTGACT GATCCTGAGC AAGAGAAGAA CTTTAGCTTC 
AGCCTTCGGG ATTCCCTGTG CCGGGCTCGG GAACGAAGAC TGCTGGAGGA CTATGAGATT 
CATGTGACCC CTGGCGTGCA GCCACCCCCC CCTCAGATGG GCGAGATCAT CAGTTGCTGT 
GGAGGCACTT TCCTGCCCAG CATGCCTCAC TCCTATAAGC TTCACCGAGT CATCATAACT 
TGCACTGAAG ACCTACCACG CTGTGCTATT CCATCCCGCC TGGGGCTACC CCTCCTCTCT 
CCTGAGTTCC TCCTGACTGG AGTGCTGAAG CAGGAAGCCA CACCAGAGGC CTTTGTTCTC 
TCCAATTTGG AAATGTCATC TACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mdc1 ( XM_006524260.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524323.1 Mus musculus mediator of DNA damage checkpoint 1 (Mdc1), transcript variant X1, mRNA.

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CloneID	OMu19129
Clone ID Related Accession (Same CDS sequence)	NM_001010833.2 , XM_006524260.1 , XM_006524260.2 , XM_006524261.3
Accession Version	XM_006524260.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	mediator of DNA damage checkpoint protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGCA CCCAGGTGAT TGACTGGGAT GCTGAAGAAG AGGAAGAGAC AGAACTATCC 
AGTGGCTCCT TGGGGTATAG TGTGGAGCCT ATAGGGCAGC TACGTCTCTT CAGTGGAACT 
CATGGACCAG AAAGAGATTT CCCCCTCTAC CTTGGCAAGA ATGTAGTTGG TCGAAGCCCT 
GACTGCTCTG TGGCCCTGCC TTTTCCATCC ATCTCCAAAC AGCATGCAGT AATTGAAATC 
TCAGCTTGGA ACAAAGCCCC TATCCTCCAG GATTGTGGGA GCCTCAATGG CACTCAAATC 
GTAAAGCCTC CTAGGGTCCT ACCTCCTGGA GTGAGCCATC GTCTGAGGGA CCAGGAATTA 
ATTCTGTTTG CAGATTTTCC CTGCCAGTAC CATCGCCTGG ATGTCCCACC ACCCTTGGTC 
CCTCGGAGTC TTCTAACTAT AGAGAAAACC CCCAGAATAC GGATAGAATC CCAAAATTCC 
AGAGTTTTGT TGGCTGCCGA TTCAGAGGAA GAAGGGGATT TTCCTTCTGG AAGGTGTGTG 
GCGAATGGAC AAAGGAATAC AGCATCCCCT TCAGCAACAG TAGTTCCAGA AAGTGATGAA 
GAGGTCTCTT CTCCGGCCCC AAGTGTCCCC GGGCCATCTT CACCCTTTGG TTTGGGCAGT 
GACACTGATG AAGAACAAGG TCAGCAACCA GGAGTAGAGG AGTCCTCTTT AGCTGACAGC 
AGTGGTGCTG CAGGGGAAGC TGAGCAGCCT GAAGCTAACG GAACGACAGC TGGCATACAG 
GCTCAGCCTA CTGAGCACAA ATTGAAGGAC ACAAAAGTCA AGAAGGAGGC AGGCAGGGCA 
GGGGTCTCAG ATGGGTCAGT TCTGGAGAGG AGCCCCACTC TTGGAGAGGA CAGTGACACA 
GAAGTGGACG AAGATCACAA ACCTGGGTTT GCGGACAGTG AGACAGATGT GGAAGAAGAG 
AGGATCCCTG TGACCCCCCC TGTAGCCCCT GTGAAGAAAA ACCAAGTGCT ACTTGCAGTT 
GGTATAGGAG ATCCTGAAGC ACCTGGTGTG GCACATCTGC AGGACTGCCT AGCTGGTAGT 
GGCACAGATG TGGAGGACAA GACTGCACTG GATGTCCCTC TAGAGAGAAA CCACACACCC 
ATGGTGATCA ACAGTGATAC AGATGAGGAG GAGGAGGAGG AGGAGGAGGA GGTCTCAGCA 
GCACTCACTT TGGCCCATCT GAAAGAGAGA GGAATTGGTT TGTGGAGTAG AGACCCAGGC 
GCAGAAGAGG TCAAGTCCCA ACCACAGGTC CTTGTAGAAC AAAGCCAGAG TGCCTCTGGG 
AGAGACAGTG ACACGGATGT GGAGGAGGAA TCCTCAGGGC GAAAGAGAGA GATTATCCCA 
GACAGTCCCA TGGATGTAGA TGAGGCTCTT ACTGTCACAC AGCCAGAGAG CCAGCCTCCC 
CGTAGGCCCA ATGATGCAGA TGAGTACATG GATATGAGCT CCCCTGGCAG CCATCTAGTG 
GTAAATCAGG CCTCCTTTGC TGTGGTAGGC AAGACCAGAG CACAAGTGGA GGAGGAAGTC 
CCAGGGCCAT CTGTTATCCT GGGGGAGAAG CACCAGGTGC CTCTAGAGGG AGCCCAGCCT 
CCTGAGGAAG CCTGGGAAAC AGCTGTTCAG GAAGGCTCAT CCTCACCAGA GGCAGCAGCC 
TCTGTAAGGC CTAGCCAGCA GCCAGTAGCA GAAGATGCTG GGACAGAGTG TGCTACAGCT 
GTTTCTGAGC AGGAAAGTAC TCTTGAGGTG AGGTCCCAAA GCGGGTCACC TGCAGCACCA 
GTGGAGCAAG TGGTGATACA TACAGATACT TCAGGGGATC CCACCCTGCC ACAGAGAGAG 
GGAGCCCAAA CCCCCACAGG AAGGGAGAGA GAAGCACATG TGGGCAGGAC CAAGAGTGCC 
AAAGAGTGCT GTGATGCAGA ACCTGAAGAT CTGTGCCTTC CAGCTACCCA GTGCTTTGTG 
GAAGGGGAGA GCCAGCACCC AGAAGCTGTC CAGAGTTTGG AAAATGAGCC TACCCAACTC 
TTCCCATGTA CTCTTCCACA AGAGCCAGGA CCTTCCCATC TCAGCCTTCA GACTCCAGGT 
GCAGATACCT TGGATGTGCC TTGGGAAGTC TTGGCTACAC AGCCGTTCTG TCTAAGAGAA 
CAGTCAGAGA CTTCTGAGCT TCATGAAGCT CATGGATCTC AACCATCTCT GCCTAGGGAA 
CCACCAGGAC ACCAACATCT GGTTCATACC AGCCCAGTTC ACACAGAGCT GTTGAGAATT 
GAAGGCAGAG AGATACAGAC TGTGGAGAAA GCCATGGGTA TACCAAAGGA AATGGCAGAC 
AGGATGACCC CTGAGAGAGA GCCATTGGAG AGGGAAATCA GGGGAAGAAC AGAAAACAGT 
GAGAGAGATG TGATAGGAGA AGAACTAATT CAGGGGACAA AGGACAGAGA GCCAAAGAAG 
GTGTTAGCTA GAGACAGTCA GAGAAAAGAG GCTGACAAGG ATCTTGAAGG GAATAGGGAG 
AGTTTGGAGG TAGAAATAGA GATGTCCAAA GACTCACAGA AGAGAGAGAG AAAAGTAGAG 
AAACCAGAAC CAAAGAGAGA ATGGGAGCCA GCTGATTTAG AAGTGACTCC AGATAGAGGG 
GTGACAGAGG AAGGGAGCCA TGATCAGAAA GGGCAAATTG CTAGTCTAAC ACTAAAGCCT 
GGAGTTGGGG TGAAGGACCT TGAGGGACTT GCATCAGCCC CAATAATCAC AGGGAGCCAG 
GCAGATGGAG GAAAGGGAGA CCCTTTGAGC CCTGGGAGGC AGCAGAGAGG CCGCTTGAGT 
TGCCAGACGA CACCTGCTGG AAAGGCTTCC AGGGGTGATC CAGAGCCCCC AGACCACTGC 
CTGTTTTCTT CAGTGCCTGA AGCTTCAACT CAGAGTCTCC TCACCTCTCA GAGCCAAAAG 
CAGTCTACAC CTCAGCCTCT GTTTTCGACC TCTTCTTCTG AGATACCCCT TCCTGAGAGT 
CTTCACACAA AGCCTAATGT CAGGCCACGG CGGTCCTCCA GGATGACCCC CTCCCCGCAT 
TCCTCTGCTG CCCTTAAGCC AAATACTACC TGCCCCACAA ACCAGCCTGC TGCCTCTAGA 
CCAACATCCC GACCCACTCG GGGCAGGGCA AATAGGTCCT CTACCAGGAC CCCAGAACTG 
ATTGTCCCTG TAGACCCTGA ACTCCAGCCT TCCACCTCCA CAGAACAGCC TGTCATCCCA 
AAACTTACGT CCCAGGTCAC TGAGGGCAGG GTACAGATGC CTGAACCACT TCTCACAGGT 
CCCGAGATCC AGTCTCCCAC CTCCACAGAA CAGTCTGTCA CCCCAGACCG CAAACCTCGG 
GCCACTCGGG GCAGGCCAAG TAAATCTCCC AACAAGACCC CAGAACCACT TATTTCTACT 
GGCCCTGAAC TCCAACCTCC CACCTCCATA GAACAACCTG TCATACCTAA ACCCACTTCT 
CGGGTCACTC GGGGCAGACC ACGTAAGTCT TCTGTCAGGA CCCCAGAATC AGTTGTCTCC 
ACTGGCCCTG AACTTCAGCC TCTCACCTCT ATAGAACAGC CTGTTATCCC TGAACCTAGG 
GCCACTCGAG GTAGACCAAG TAAGTCTTCT ATCAAGACCC CAGAATCAGT TGTCCCCACT 
GGTCCTGAGC TGCAGCCTCT TACCTCTGCA AAACAGCCTG TCACCCCTAA CCTCACATCT 
CGGGCCTCTC GGGGTAGATC AAGTAAGTCT ATCAGGACCC CGGAACCAGT TGTCCAAACT 
GGCCCTGAAT TCCATCCATC CACCTCCACA GAACAGCCTG ATACCCGTGA GCCCTCATCT 
CAGGCTAGGA CACGTAGGTC TGCTGTCAAG ACTCCTGAAG CGAGTGTCCC CACAACCCCT 
GAACTCCAGC CTTTCACTTC TAAAAAACAG CCTGCCCCTA AGCCTACAGC TCTGGTCACT 
CAGGGAAGGA CATATAAACC CTCCACTGAA GATTGTGAAT CAGTTGGGCC AGTGGCCCCT 
GATTTTGAGC CTTCCACCTC TACAGACCAT CTTGTCACCC CTAAGGTTAC AGATCAGAGC 
TTAACACTAC AGTCTTCACC CCTAAGTGCT TCCCCAGTTT CCAGTACCCC TGATCTCAAG 
CCTCCTGTCC CCATAGCCCA GCCTGTTACC CCAGAGCCCA TTCCACAAGC CAATCACCAA 
AGGAAACGAA GGGCTGCTGG GAAGCAGGGT TCCCGCACAG TCCCTCTGGG TCATAAGTCT 
TACTCTGCCC TCTCTGAACC TGAGCCCCAA TCTTCAGCCA GCCAAAGCTC AGGAGCATCA 
GAAGCAGACT CACCCCGTCA AAAACGTCCC AGAAGACAAG CCTCCCAGAA AACTGTAGTC 
ATCAAGGAAG AACCTGTAGA AACAGAAGTG AAGGAAGAGC CTCAAGAGAC TGCAATTCCA 
ACACCAGAAA AGAGAAAGAG GGACCATGCA GAAGAAGTGA CCCAGGGAAA ACCAACTCGA 
AGCCGGCGAA CTAAACCTAA CCAAGAAACA GCCCCCAAGG TACTGTTCAC AGGAGTCATG 
GATTCTCGTG GCGAGCGTGC AGTGCTGGCT CTGGGAGGCA GTCTGGCCAG CTCAGTAAAT 
GAGGCCTCCC ACTTAGTTAC TGATCGCATC CGCAGGACAG TCAAGTTCTT GTGTGCCCTG 
GGGAAGGGAA TTCCCATCCT GTCCCTGAAC TGGCTGTACC AGTCCCGAAA GGCTGGTTGC 
TTCTTGCCGC CTGATGACTA CTTAGTGACT GATCCTGAGC AAGAGAAGAA CTTTAGCTTC 
AGCCTTCGGG ATTCCCTGTG CCGGGCTCGG GAACGAAGAC TGCTGGAGGA CTATGAGATT 
CATGTGACCC CTGGCGTGCA GCCACCCCCC CCTCAGATGG GCGAGATCAT CAGTTGCTGT 
GGAGGCACTT TCCTGCCCAG CATGCCTCAC TCCTATAAGC TTCACCGAGT CATCATAACT 
TGCACTGAAG ACCTACCACG CTGTGCTATT CCATCCCGCC TGGGGCTACC CCTCCTCTCT 
CCTGAGTTCC TCCTGACTGG AGTGCTGAAG CAGGAAGCCA CACCAGAGGC CTTTGTTCTC 
TCCAATTTGG AAATGTCATC TACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524323.1
CDS	94..5220
Translation	MESTQVIDWDAEEEEETELSSGSLGYSVEPIGQLRLFSGTHGPERDFPLYLGKNVVGRSPDCSVALPFPSISKQHAVIEISAWNKAPILQDCGSLNGTQIVKPPRVLPPGVSHRLRDQELILFADFPCQYHRLDVPPPLVPRSLLTIEKTPRIRIESQNSRVLLAADSEEEGDFPSGRCVANGQRNTASPSATVVPESDEEVSSPAPSVPGPSSPFGLGSDTDEEQGQQPGVEESSLADSSGAAGEAEQPEANGTTAGIQAQPTEHKLKDTKVKKEAGRAGVSDGSVLERSPTLGEDSDTEVDEDHKPGFADSETDVEEERIPVTPPVAPVKKNQVLLAVGIGDPEAPGVAHLQDCLAGSGTDVEDKTALDVPLERNHTPMVINSDTDEEEEEEEEEVSAALTLAHLKERGIGLWSRDPGAEEVKSQPQVLVEQSQSASGRDSDTDVEEESSGRKREIIPDSPMDVDEALTVTQPESQPPRRPNDADEYMDMSSPGSHLVVNQASFAVVGKTRAQVEEEVPGPSVILGEKHQVPLEGAQPPEEAWETAVQEGSSSPEAAASVRPSQQPVAEDAGTECATAVSEQESTLEVRSQSGSPAAPVEQVVIHTDTSGDPTLPQREGAQTPTGREREAHVGRTKSAKECCDAEPEDLCLPATQCFVEGESQHPEAVQSLENEPTQLFPCTLPQEPGPSHLSLQTPGADTLDVPWEVLATQPFCLREQSETSELHEAHGSQPSLPREPPGHQHLVHTSPVHTELLRIEGREIQTVEKAMGIPKEMADRMTPEREPLEREIRGRTENSERDVIGEELIQGTKDREPKKVLARDSQRKEADKDLEGNRESLEVEIEMSKDSQKRERKVEKPEPKREWEPADLEVTPDRGVTEEGSHDQKGQIASLTLKPGVGVKDLEGLASAPIITGSQADGGKGDPLSPGRQQRGRLSCQTTPAGKASRGDPEPPDHCLFSSVPEASTQSLLTSQSQKQSTPQPLFSTSSSEIPLPESLHTKPNVRPRRSSRMTPSPHSSAALKPNTTCPTNQPAASRPTSRPTRGRANRSSTRTPELIVPVDPELQPSTSTEQPVIPKLTSQVTEGRVQMPEPLLTGPEIQSPTSTEQSVTPDRKPRATRGRPSKSPNKTPEPLISTGPELQPPTSIEQPVIPKPTSRVTRGRPRKSSVRTPESVVSTGPELQPLTSIEQPVIPEPRATRGRPSKSSIKTPESVVPTGPELQPLTSAKQPVTPNLTSRASRGRSSKSIRTPEPVVQTGPEFHPSTSTEQPDTREPSSQARTRRSAVKTPEASVPTTPELQPFTSKKQPAPKPTALVTQGRTYKPSTEDCESVGPVAPDFEPSTSTDHLVTPKVTDQSLTLQSSPLSASPVSSTPDLKPPVPIAQPVTPEPIPQANHQRKRRAAGKQGSRTVPLGHKSYSALSEPEPQSSASQSSGASEADSPRQKRPRRQASQKTVVIKEEPVETEVKEEPQETAIPTPEKRKRDHAEEVTQGKPTRSRRTKPNQETAPKVLFTGVMDSRGERAVLALGGSLASSVNEASHLVTDRIRRTVKFLCALGKGIPILSLNWLYQSRKAGCFLPPDDYLVTDPEQEKNFSFSLRDSLCRARERRLLEDYEIHVTPGVQPPPPQMGEIISCCGGTFLPSMPHSYKLHRVIITCTEDLPRCAIPSRLGLPLLSPEFLLTGVLKQEATPEAFVLSNLEMSST

Target ORF information:

RefSeq Version	XM_006524260.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mediator of DNA damage checkpoint 1 (Mdc1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524260.1

ORF Insert Sequence:

ATGGAAAGCA CCCAGGTGAT TGACTGGGAT GCTGAAGAAG AGGAAGAGAC AGAACTATCC 
AGTGGCTCCT TGGGGTATAG TGTGGAGCCT ATAGGGCAGC TACGTCTCTT CAGTGGAACT 
CATGGACCAG AAAGAGATTT CCCCCTCTAC CTTGGCAAGA ATGTAGTTGG TCGAAGCCCT 
GACTGCTCTG TGGCCCTGCC TTTTCCATCC ATCTCCAAAC AGCATGCAGT AATTGAAATC 
TCAGCTTGGA ACAAAGCCCC TATCCTCCAG GATTGTGGGA GCCTCAATGG CACTCAAATC 
GTAAAGCCTC CTAGGGTCCT ACCTCCTGGA GTGAGCCATC GTCTGAGGGA CCAGGAATTA 
ATTCTGTTTG CAGATTTTCC CTGCCAGTAC CATCGCCTGG ATGTCCCACC ACCCTTGGTC 
CCTCGGAGTC TTCTAACTAT AGAGAAAACC CCCAGAATAC GGATAGAATC CCAAAATTCC 
AGAGTTTTGT TGGCTGCCGA TTCAGAGGAA GAAGGGGATT TTCCTTCTGG AAGGTGTGTG 
GCGAATGGAC AAAGGAATAC AGCATCCCCT TCAGCAACAG TAGTTCCAGA AAGTGATGAA 
GAGGTCTCTT CTCCGGCCCC AAGTGTCCCC GGGCCATCTT CACCCTTTGG TTTGGGCAGT 
GACACTGATG AAGAACAAGG TCAGCAACCA GGAGTAGAGG AGTCCTCTTT AGCTGACAGC 
AGTGGTGCTG CAGGGGAAGC TGAGCAGCCT GAAGCTAACG GAACGACAGC TGGCATACAG 
GCTCAGCCTA CTGAGCACAA ATTGAAGGAC ACAAAAGTCA AGAAGGAGGC AGGCAGGGCA 
GGGGTCTCAG ATGGGTCAGT TCTGGAGAGG AGCCCCACTC TTGGAGAGGA CAGTGACACA 
GAAGTGGACG AAGATCACAA ACCTGGGTTT GCGGACAGTG AGACAGATGT GGAAGAAGAG 
AGGATCCCTG TGACCCCCCC TGTAGCCCCT GTGAAGAAAA ACCAAGTGCT ACTTGCAGTT 
GGTATAGGAG ATCCTGAAGC ACCTGGTGTG GCACATCTGC AGGACTGCCT AGCTGGTAGT 
GGCACAGATG TGGAGGACAA GACTGCACTG GATGTCCCTC TAGAGAGAAA CCACACACCC 
ATGGTGATCA ACAGTGATAC AGATGAGGAG GAGGAGGAGG AGGAGGAGGA GGTCTCAGCA 
GCACTCACTT TGGCCCATCT GAAAGAGAGA GGAATTGGTT TGTGGAGTAG AGACCCAGGC 
GCAGAAGAGG TCAAGTCCCA ACCACAGGTC CTTGTAGAAC AAAGCCAGAG TGCCTCTGGG 
AGAGACAGTG ACACGGATGT GGAGGAGGAA TCCTCAGGGC GAAAGAGAGA GATTATCCCA 
GACAGTCCCA TGGATGTAGA TGAGGCTCTT ACTGTCACAC AGCCAGAGAG CCAGCCTCCC 
CGTAGGCCCA ATGATGCAGA TGAGTACATG GATATGAGCT CCCCTGGCAG CCATCTAGTG 
GTAAATCAGG CCTCCTTTGC TGTGGTAGGC AAGACCAGAG CACAAGTGGA GGAGGAAGTC 
CCAGGGCCAT CTGTTATCCT GGGGGAGAAG CACCAGGTGC CTCTAGAGGG AGCCCAGCCT 
CCTGAGGAAG CCTGGGAAAC AGCTGTTCAG GAAGGCTCAT CCTCACCAGA GGCAGCAGCC 
TCTGTAAGGC CTAGCCAGCA GCCAGTAGCA GAAGATGCTG GGACAGAGTG TGCTACAGCT 
GTTTCTGAGC AGGAAAGTAC TCTTGAGGTG AGGTCCCAAA GCGGGTCACC TGCAGCACCA 
GTGGAGCAAG TGGTGATACA TACAGATACT TCAGGGGATC CCACCCTGCC ACAGAGAGAG 
GGAGCCCAAA CCCCCACAGG AAGGGAGAGA GAAGCACATG TGGGCAGGAC CAAGAGTGCC 
AAAGAGTGCT GTGATGCAGA ACCTGAAGAT CTGTGCCTTC CAGCTACCCA GTGCTTTGTG 
GAAGGGGAGA GCCAGCACCC AGAAGCTGTC CAGAGTTTGG AAAATGAGCC TACCCAACTC 
TTCCCATGTA CTCTTCCACA AGAGCCAGGA CCTTCCCATC TCAGCCTTCA GACTCCAGGT 
GCAGATACCT TGGATGTGCC TTGGGAAGTC TTGGCTACAC AGCCGTTCTG TCTAAGAGAA 
CAGTCAGAGA CTTCTGAGCT TCATGAAGCT CATGGATCTC AACCATCTCT GCCTAGGGAA 
CCACCAGGAC ACCAACATCT GGTTCATACC AGCCCAGTTC ACACAGAGCT GTTGAGAATT 
GAAGGCAGAG AGATACAGAC TGTGGAGAAA GCCATGGGTA TACCAAAGGA AATGGCAGAC 
AGGATGACCC CTGAGAGAGA GCCATTGGAG AGGGAAATCA GGGGAAGAAC AGAAAACAGT 
GAGAGAGATG TGATAGGAGA AGAACTAATT CAGGGGACAA AGGACAGAGA GCCAAAGAAG 
GTGTTAGCTA GAGACAGTCA GAGAAAAGAG GCTGACAAGG ATCTTGAAGG GAATAGGGAG 
AGTTTGGAGG TAGAAATAGA GATGTCCAAA GACTCACAGA AGAGAGAGAG AAAAGTAGAG 
AAACCAGAAC CAAAGAGAGA ATGGGAGCCA GCTGATTTAG AAGTGACTCC AGATAGAGGG 
GTGACAGAGG AAGGGAGCCA TGATCAGAAA GGGCAAATTG CTAGTCTAAC ACTAAAGCCT 
GGAGTTGGGG TGAAGGACCT TGAGGGACTT GCATCAGCCC CAATAATCAC AGGGAGCCAG 
GCAGATGGAG GAAAGGGAGA CCCTTTGAGC CCTGGGAGGC AGCAGAGAGG CCGCTTGAGT 
TGCCAGACGA CACCTGCTGG AAAGGCTTCC AGGGGTGATC CAGAGCCCCC AGACCACTGC 
CTGTTTTCTT CAGTGCCTGA AGCTTCAACT CAGAGTCTCC TCACCTCTCA GAGCCAAAAG 
CAGTCTACAC CTCAGCCTCT GTTTTCGACC TCTTCTTCTG AGATACCCCT TCCTGAGAGT 
CTTCACACAA AGCCTAATGT CAGGCCACGG CGGTCCTCCA GGATGACCCC CTCCCCGCAT 
TCCTCTGCTG CCCTTAAGCC AAATACTACC TGCCCCACAA ACCAGCCTGC TGCCTCTAGA 
CCAACATCCC GACCCACTCG GGGCAGGGCA AATAGGTCCT CTACCAGGAC CCCAGAACTG 
ATTGTCCCTG TAGACCCTGA ACTCCAGCCT TCCACCTCCA CAGAACAGCC TGTCATCCCA 
AAACTTACGT CCCAGGTCAC TGAGGGCAGG GTACAGATGC CTGAACCACT TCTCACAGGT 
CCCGAGATCC AGTCTCCCAC CTCCACAGAA CAGTCTGTCA CCCCAGACCG CAAACCTCGG 
GCCACTCGGG GCAGGCCAAG TAAATCTCCC AACAAGACCC CAGAACCACT TATTTCTACT 
GGCCCTGAAC TCCAACCTCC CACCTCCATA GAACAACCTG TCATACCTAA ACCCACTTCT 
CGGGTCACTC GGGGCAGACC ACGTAAGTCT TCTGTCAGGA CCCCAGAATC AGTTGTCTCC 
ACTGGCCCTG AACTTCAGCC TCTCACCTCT ATAGAACAGC CTGTTATCCC TGAACCTAGG 
GCCACTCGAG GTAGACCAAG TAAGTCTTCT ATCAAGACCC CAGAATCAGT TGTCCCCACT 
GGTCCTGAGC TGCAGCCTCT TACCTCTGCA AAACAGCCTG TCACCCCTAA CCTCACATCT 
CGGGCCTCTC GGGGTAGATC AAGTAAGTCT ATCAGGACCC CGGAACCAGT TGTCCAAACT 
GGCCCTGAAT TCCATCCATC CACCTCCACA GAACAGCCTG ATACCCGTGA GCCCTCATCT 
CAGGCTAGGA CACGTAGGTC TGCTGTCAAG ACTCCTGAAG CGAGTGTCCC CACAACCCCT 
GAACTCCAGC CTTTCACTTC TAAAAAACAG CCTGCCCCTA AGCCTACAGC TCTGGTCACT 
CAGGGAAGGA CATATAAACC CTCCACTGAA GATTGTGAAT CAGTTGGGCC AGTGGCCCCT 
GATTTTGAGC CTTCCACCTC TACAGACCAT CTTGTCACCC CTAAGGTTAC AGATCAGAGC 
TTAACACTAC AGTCTTCACC CCTAAGTGCT TCCCCAGTTT CCAGTACCCC TGATCTCAAG 
CCTCCTGTCC CCATAGCCCA GCCTGTTACC CCAGAGCCCA TTCCACAAGC CAATCACCAA 
AGGAAACGAA GGGCTGCTGG GAAGCAGGGT TCCCGCACAG TCCCTCTGGG TCATAAGTCT 
TACTCTGCCC TCTCTGAACC TGAGCCCCAA TCTTCAGCCA GCCAAAGCTC AGGAGCATCA 
GAAGCAGACT CACCCCGTCA AAAACGTCCC AGAAGACAAG CCTCCCAGAA AACTGTAGTC 
ATCAAGGAAG AACCTGTAGA AACAGAAGTG AAGGAAGAGC CTCAAGAGAC TGCAATTCCA 
ACACCAGAAA AGAGAAAGAG GGACCATGCA GAAGAAGTGA CCCAGGGAAA ACCAACTCGA 
AGCCGGCGAA CTAAACCTAA CCAAGAAACA GCCCCCAAGG TACTGTTCAC AGGAGTCATG 
GATTCTCGTG GCGAGCGTGC AGTGCTGGCT CTGGGAGGCA GTCTGGCCAG CTCAGTAAAT 
GAGGCCTCCC ACTTAGTTAC TGATCGCATC CGCAGGACAG TCAAGTTCTT GTGTGCCCTG 
GGGAAGGGAA TTCCCATCCT GTCCCTGAAC TGGCTGTACC AGTCCCGAAA GGCTGGTTGC 
TTCTTGCCGC CTGATGACTA CTTAGTGACT GATCCTGAGC AAGAGAAGAA CTTTAGCTTC 
AGCCTTCGGG ATTCCCTGTG CCGGGCTCGG GAACGAAGAC TGCTGGAGGA CTATGAGATT 
CATGTGACCC CTGGCGTGCA GCCACCCCCC CCTCAGATGG GCGAGATCAT CAGTTGCTGT 
GGAGGCACTT TCCTGCCCAG CATGCCTCAC TCCTATAAGC TTCACCGAGT CATCATAACT 
TGCACTGAAG ACCTACCACG CTGTGCTATT CCATCCCGCC TGGGGCTACC CCTCCTCTCT 
CCTGAGTTCC TCCTGACTGG AGTGCTGAAG CAGGAAGCCA CACCAGAGGC CTTTGTTCTC 
TCCAATTTGG AAATGTCATC TACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mdc1 ( XM_006524261.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524324.1 Mus musculus mediator of DNA damage checkpoint 1 (Mdc1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu19129
Clone ID Related Accession (Same CDS sequence)	NM_001010833.2 , XM_006524260.1 , XM_006524260.2 , XM_006524261.3
Accession Version	XM_006524261.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	mediator of DNA damage checkpoint protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006524261.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGCA CCCAGGTGAT TGACTGGGAT GCTGAAGAAG AGGAAGAGAC AGAACTATCC 
AGTGGCTCCT TGGGGTATAG TGTGGAGCCT ATAGGGCAGC TACGTCTCTT CAGTGGAACT 
CATGGACCAG AAAGAGATTT CCCCCTCTAC CTTGGCAAGA ATGTAGTTGG TCGAAGCCCT 
GACTGCTCTG TGGCCCTGCC TTTTCCATCC ATCTCCAAAC AGCATGCAGT AATTGAAATC 
TCAGCTTGGA ACAAAGCCCC TATCCTCCAG GATTGTGGGA GCCTCAATGG CACTCAAATC 
GTAAAGCCTC CTAGGGTCCT ACCTCCTGGA GTGAGCCATC GTCTGAGGGA CCAGGAATTA 
ATTCTGTTTG CAGATTTTCC CTGCCAGTAC CATCGCCTGG ATGTCCCACC ACCCTTGGTC 
CCTCGGAGTC TTCTAACTAT AGAGAAAACC CCCAGAATAC GGATAGAATC CCAAAATTCC 
AGAGTTTTGT TGGCTGCCGA TTCAGAGGAA GAAGGGGATT TTCCTTCTGG AAGGTGTGTG 
GCGAATGGAC AAAGGAATAC AGCATCCCCT TCAGCAACAG TAGTTCCAGA AAGTGATGAA 
GAGGTCTCTT CTCCGGCCCC AAGTGTCCCC GGGCCATCTT CACCCTTTGG TTTGGGCAGT 
GACACTGATG AAGAACAAGG TCAGCAACCA GGAGTAGAGG AGTCCTCTTT AGCTGACAGC 
AGTGGTGCTG CAGGGGAAGC TGAGCAGCCT GAAGCTAACG GAACGACAGC TGGCATACAG 
GCTCAGCCTA CTGAGCACAA ATTGAAGGAC ACAAAAGTCA AGAAGGAGGC AGGCAGGGCA 
GGGGTCTCAG ATGGGTCAGT TCTGGAGAGG AGCCCCACTC TTGGAGAGGA CAGTGACACA 
GAAGTGGACG AAGATCACAA ACCTGGGTTT GCGGACAGTG AGACAGATGT GGAAGAAGAG 
AGGATCCCTG TGACCCCCCC TGTAGCCCCT GTGAAGAAAA ACCAAGTGCT ACTTGCAGTT 
GGTATAGGAG ATCCTGAAGC ACCTGGTGTG GCACATCTGC AGGACTGCCT AGCTGGTAGT 
GGCACAGATG TGGAGGACAA GACTGCACTG GATGTCCCTC TAGAGAGAAA CCACACACCC 
ATGGTGATCA ACAGTGATAC AGATGAGGAG GAGGAGGAGG AGGAGGAGGA GGTCTCAGCA 
GCACTCACTT TGGCCCATCT GAAAGAGAGA GGAATTGGTT TGTGGAGTAG AGACCCAGGC 
GCAGAAGAGG TCAAGTCCCA ACCACAGGTC CTTGTAGAAC AAAGCCAGAG TGCCTCTGGG 
AGAGACAGTG ACACGGATGT GGAGGAGGAA TCCTCAGGGC GAAAGAGAGA GATTATCCCA 
GACAGTCCCA TGGATGTAGA TGAGGCTCTT ACTGTCACAC AGCCAGAGAG CCAGCCTCCC 
CGTAGGCCCA ATGATGCAGA TGAGTACATG GATATGAGCT CCCCTGGCAG CCATCTAGTG 
GTAAATCAGG CCTCCTTTGC TGTGGTAGGC AAGACCAGAG CACAAGTGGA GGAGGAAGTC 
CCAGGGCCAT CTGTTATCCT GGGGGAGAAG CACCAGGTGC CTCTAGAGGG AGCCCAGCCT 
CCTGAGGAAG CCTGGGAAAC AGCTGTTCAG GAAGGCTCAT CCTCACCAGA GGCAGCAGCC 
TCTGTAAGGC CTAGCCAGCA GCCAGTAGCA GAAGATGCTG GGACAGAGTG TGCTACAGCT 
GTTTCTGAGC AGGAAAGTAC TCTTGAGGTG AGGTCCCAAA GCGGGTCACC TGCAGCACCA 
GTGGAGCAAG TGGTGATACA TACAGATACT TCAGGGGATC CCACCCTGCC ACAGAGAGAG 
GGAGCCCAAA CCCCCACAGG AAGGGAGAGA GAAGCACATG TGGGCAGGAC CAAGAGTGCC 
AAAGAGTGCT GTGATGCAGA ACCTGAAGAT CTGTGCCTTC CAGCTACCCA GTGCTTTGTG 
GAAGGGGAGA GCCAGCACCC AGAAGCTGTC CAGAGTTTGG AAAATGAGCC TACCCAACTC 
TTCCCATGTA CTCTTCCACA AGAGCCAGGA CCTTCCCATC TCAGCCTTCA GACTCCAGGT 
GCAGATACCT TGGATGTGCC TTGGGAAGTC TTGGCTACAC AGCCGTTCTG TCTAAGAGAA 
CAGTCAGAGA CTTCTGAGCT TCATGAAGCT CATGGATCTC AACCATCTCT GCCTAGGGAA 
CCACCAGGAC ACCAACATCT GGTTCATACC AGCCCAGTTC ACACAGAGCT GTTGAGAATT 
GAAGGCAGAG AGATACAGAC TGTGGAGAAA GCCATGGGTA TACCAAAGGA AATGGCAGAC 
AGGATGACCC CTGAGAGAGA GCCATTGGAG AGGGAAATCA GGGGAAGAAC AGAAAACAGT 
GAGAGAGATG TGATAGGAGA AGAACTAATT CAGGGGACAA AGGACAGAGA GCCAAAGAAG 
GTGTTAGCTA GAGACAGTCA GAGAAAAGAG GCTGACAAGG ATCTTGAAGG GAATAGGGAG 
AGTTTGGAGG TAGAAATAGA GATGTCCAAA GACTCACAGA AGAGAGAGAG AAAAGTAGAG 
AAACCAGAAC CAAAGAGAGA ATGGGAGCCA GCTGATTTAG AAGTGACTCC AGATAGAGGG 
GTGACAGAGG AAGGGAGCCA TGATCAGAAA GGGCAAATTG CTAGTCTAAC ACTAAAGCCT 
GGAGTTGGGG TGAAGGACCT TGAGGGACTT GCATCAGCCC CAATAATCAC AGGGAGCCAG 
GCAGATGGAG GAAAGGGAGA CCCTTTGAGC CCTGGGAGGC AGCAGAGAGG CCGCTTGAGT 
TGCCAGACGA CACCTGCTGG AAAGGCTTCC AGGGGTGATC CAGAGCCCCC AGACCACTGC 
CTGTTTTCTT CAGTGCCTGA AGCTTCAACT CAGAGTCTCC TCACCTCTCA GAGCCAAAAG 
CAGTCTACAC CTCAGCCTCT GTTTTCGACC TCTTCTTCTG AGATACCCCT TCCTGAGAGT 
CTTCACACAA AGCCTAATGT CAGGCCACGG CGGTCCTCCA GGATGACCCC CTCCCCGCAT 
TCCTCTGCTG CCCTTAAGCC AAATACTACC TGCCCCACAA ACCAGCCTGC TGCCTCTAGA 
CCAACATCCC GACCCACTCG GGGCAGGGCA AATAGGTCCT CTACCAGGAC CCCAGAACTG 
ATTGTCCCTG TAGACCCTGA ACTCCAGCCT TCCACCTCCA CAGAACAGCC TGTCATCCCA 
AAACTTACGT CCCAGGTCAC TGAGGGCAGG GTACAGATGC CTGAACCACT TCTCACAGGT 
CCCGAGATCC AGTCTCCCAC CTCCACAGAA CAGTCTGTCA CCCCAGACCG CAAACCTCGG 
GCCACTCGGG GCAGGCCAAG TAAATCTCCC AACAAGACCC CAGAACCACT TATTTCTACT 
GGCCCTGAAC TCCAACCTCC CACCTCCATA GAACAACCTG TCATACCTAA ACCCACTTCT 
CGGGTCACTC GGGGCAGACC ACGTAAGTCT TCTGTCAGGA CCCCAGAATC AGTTGTCTCC 
ACTGGCCCTG AACTTCAGCC TCTCACCTCT ATAGAACAGC CTGTTATCCC TGAACCTAGG 
GCCACTCGAG GTAGACCAAG TAAGTCTTCT ATCAAGACCC CAGAATCAGT TGTCCCCACT 
GGTCCTGAGC TGCAGCCTCT TACCTCTGCA AAACAGCCTG TCACCCCTAA CCTCACATCT 
CGGGCCTCTC GGGGTAGATC AAGTAAGTCT ATCAGGACCC CGGAACCAGT TGTCCAAACT 
GGCCCTGAAT TCCATCCATC CACCTCCACA GAACAGCCTG ATACCCGTGA GCCCTCATCT 
CAGGCTAGGA CACGTAGGTC TGCTGTCAAG ACTCCTGAAG CGAGTGTCCC CACAACCCCT 
GAACTCCAGC CTTTCACTTC TAAAAAACAG CCTGCCCCTA AGCCTACAGC TCTGGTCACT 
CAGGGAAGGA CATATAAACC CTCCACTGAA GATTGTGAAT CAGTTGGGCC AGTGGCCCCT 
GATTTTGAGC CTTCCACCTC TACAGACCAT CTTGTCACCC CTAAGGTTAC AGATCAGAGC 
TTAACACTAC AGTCTTCACC CCTAAGTGCT TCCCCAGTTT CCAGTACCCC TGATCTCAAG 
CCTCCTGTCC CCATAGCCCA GCCTGTTACC CCAGAGCCCA TTCCACAAGC CAATCACCAA 
AGGAAACGAA GGGCTGCTGG GAAGCAGGGT TCCCGCACAG TCCCTCTGGG TCATAAGTCT 
TACTCTGCCC TCTCTGAACC TGAGCCCCAA TCTTCAGCCA GCCAAAGCTC AGGAGCATCA 
GAAGCAGACT CACCCCGTCA AAAACGTCCC AGAAGACAAG CCTCCCAGAA AACTGTAGTC 
ATCAAGGAAG AACCTGTAGA AACAGAAGTG AAGGAAGAGC CTCAAGAGAC TGCAATTCCA 
ACACCAGAAA AGAGAAAGAG GGACCATGCA GAAGAAGTGA CCCAGGGAAA ACCAACTCGA 
AGCCGGCGAA CTAAACCTAA CCAAGAAACA GCCCCCAAGG TACTGTTCAC AGGAGTCATG 
GATTCTCGTG GCGAGCGTGC AGTGCTGGCT CTGGGAGGCA GTCTGGCCAG CTCAGTAAAT 
GAGGCCTCCC ACTTAGTTAC TGATCGCATC CGCAGGACAG TCAAGTTCTT GTGTGCCCTG 
GGGAAGGGAA TTCCCATCCT GTCCCTGAAC TGGCTGTACC AGTCCCGAAA GGCTGGTTGC 
TTCTTGCCGC CTGATGACTA CTTAGTGACT GATCCTGAGC AAGAGAAGAA CTTTAGCTTC 
AGCCTTCGGG ATTCCCTGTG CCGGGCTCGG GAACGAAGAC TGCTGGAGGA CTATGAGATT 
CATGTGACCC CTGGCGTGCA GCCACCCCCC CCTCAGATGG GCGAGATCAT CAGTTGCTGT 
GGAGGCACTT TCCTGCCCAG CATGCCTCAC TCCTATAAGC TTCACCGAGT CATCATAACT 
TGCACTGAAG ACCTACCACG CTGTGCTATT CCATCCCGCC TGGGGCTACC CCTCCTCTCT 
CCTGAGTTCC TCCTGACTGG AGTGCTGAAG CAGGAAGCCA CACCAGAGGC CTTTGTTCTC 
TCCAATTTGG AAATGTCATC TACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524324.1
CDS	96..5222
Translation	MESTQVIDWDAEEEEETELSSGSLGYSVEPIGQLRLFSGTHGPERDFPLYLGKNVVGRSPDCSVALPFPSISKQHAVIEISAWNKAPILQDCGSLNGTQIVKPPRVLPPGVSHRLRDQELILFADFPCQYHRLDVPPPLVPRSLLTIEKTPRIRIESQNSRVLLAADSEEEGDFPSGRCVANGQRNTASPSATVVPESDEEVSSPAPSVPGPSSPFGLGSDTDEEQGQQPGVEESSLADSSGAAGEAEQPEANGTTAGIQAQPTEHKLKDTKVKKEAGRAGVSDGSVLERSPTLGEDSDTEVDEDHKPGFADSETDVEEERIPVTPPVAPVKKNQVLLAVGIGDPEAPGVAHLQDCLAGSGTDVEDKTALDVPLERNHTPMVINSDTDEEEEEEEEEVSAALTLAHLKERGIGLWSRDPGAEEVKSQPQVLVEQSQSASGRDSDTDVEEESSGRKREIIPDSPMDVDEALTVTQPESQPPRRPNDADEYMDMSSPGSHLVVNQASFAVVGKTRAQVEEEVPGPSVILGEKHQVPLEGAQPPEEAWETAVQEGSSSPEAAASVRPSQQPVAEDAGTECATAVSEQESTLEVRSQSGSPAAPVEQVVIHTDTSGDPTLPQREGAQTPTGREREAHVGRTKSAKECCDAEPEDLCLPATQCFVEGESQHPEAVQSLENEPTQLFPCTLPQEPGPSHLSLQTPGADTLDVPWEVLATQPFCLREQSETSELHEAHGSQPSLPREPPGHQHLVHTSPVHTELLRIEGREIQTVEKAMGIPKEMADRMTPEREPLEREIRGRTENSERDVIGEELIQGTKDREPKKVLARDSQRKEADKDLEGNRESLEVEIEMSKDSQKRERKVEKPEPKREWEPADLEVTPDRGVTEEGSHDQKGQIASLTLKPGVGVKDLEGLASAPIITGSQADGGKGDPLSPGRQQRGRLSCQTTPAGKASRGDPEPPDHCLFSSVPEASTQSLLTSQSQKQSTPQPLFSTSSSEIPLPESLHTKPNVRPRRSSRMTPSPHSSAALKPNTTCPTNQPAASRPTSRPTRGRANRSSTRTPELIVPVDPELQPSTSTEQPVIPKLTSQVTEGRVQMPEPLLTGPEIQSPTSTEQSVTPDRKPRATRGRPSKSPNKTPEPLISTGPELQPPTSIEQPVIPKPTSRVTRGRPRKSSVRTPESVVSTGPELQPLTSIEQPVIPEPRATRGRPSKSSIKTPESVVPTGPELQPLTSAKQPVTPNLTSRASRGRSSKSIRTPEPVVQTGPEFHPSTSTEQPDTREPSSQARTRRSAVKTPEASVPTTPELQPFTSKKQPAPKPTALVTQGRTYKPSTEDCESVGPVAPDFEPSTSTDHLVTPKVTDQSLTLQSSPLSASPVSSTPDLKPPVPIAQPVTPEPIPQANHQRKRRAAGKQGSRTVPLGHKSYSALSEPEPQSSASQSSGASEADSPRQKRPRRQASQKTVVIKEEPVETEVKEEPQETAIPTPEKRKRDHAEEVTQGKPTRSRRTKPNQETAPKVLFTGVMDSRGERAVLALGGSLASSVNEASHLVTDRIRRTVKFLCALGKGIPILSLNWLYQSRKAGCFLPPDDYLVTDPEQEKNFSFSLRDSLCRARERRLLEDYEIHVTPGVQPPPPQMGEIISCCGGTFLPSMPHSYKLHRVIITCTEDLPRCAIPSRLGLPLLSPEFLLTGVLKQEATPEAFVLSNLEMSST

Target ORF information:

RefSeq Version	XM_006524261.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus mediator of DNA damage checkpoint 1 (Mdc1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524261.3

ORF Insert Sequence:

ATGGAAAGCA CCCAGGTGAT TGACTGGGAT GCTGAAGAAG AGGAAGAGAC AGAACTATCC 
AGTGGCTCCT TGGGGTATAG TGTGGAGCCT ATAGGGCAGC TACGTCTCTT CAGTGGAACT 
CATGGACCAG AAAGAGATTT CCCCCTCTAC CTTGGCAAGA ATGTAGTTGG TCGAAGCCCT 
GACTGCTCTG TGGCCCTGCC TTTTCCATCC ATCTCCAAAC AGCATGCAGT AATTGAAATC 
TCAGCTTGGA ACAAAGCCCC TATCCTCCAG GATTGTGGGA GCCTCAATGG CACTCAAATC 
GTAAAGCCTC CTAGGGTCCT ACCTCCTGGA GTGAGCCATC GTCTGAGGGA CCAGGAATTA 
ATTCTGTTTG CAGATTTTCC CTGCCAGTAC CATCGCCTGG ATGTCCCACC ACCCTTGGTC 
CCTCGGAGTC TTCTAACTAT AGAGAAAACC CCCAGAATAC GGATAGAATC CCAAAATTCC 
AGAGTTTTGT TGGCTGCCGA TTCAGAGGAA GAAGGGGATT TTCCTTCTGG AAGGTGTGTG 
GCGAATGGAC AAAGGAATAC AGCATCCCCT TCAGCAACAG TAGTTCCAGA AAGTGATGAA 
GAGGTCTCTT CTCCGGCCCC AAGTGTCCCC GGGCCATCTT CACCCTTTGG TTTGGGCAGT 
GACACTGATG AAGAACAAGG TCAGCAACCA GGAGTAGAGG AGTCCTCTTT AGCTGACAGC 
AGTGGTGCTG CAGGGGAAGC TGAGCAGCCT GAAGCTAACG GAACGACAGC TGGCATACAG 
GCTCAGCCTA CTGAGCACAA ATTGAAGGAC ACAAAAGTCA AGAAGGAGGC AGGCAGGGCA 
GGGGTCTCAG ATGGGTCAGT TCTGGAGAGG AGCCCCACTC TTGGAGAGGA CAGTGACACA 
GAAGTGGACG AAGATCACAA ACCTGGGTTT GCGGACAGTG AGACAGATGT GGAAGAAGAG 
AGGATCCCTG TGACCCCCCC TGTAGCCCCT GTGAAGAAAA ACCAAGTGCT ACTTGCAGTT 
GGTATAGGAG ATCCTGAAGC ACCTGGTGTG GCACATCTGC AGGACTGCCT AGCTGGTAGT 
GGCACAGATG TGGAGGACAA GACTGCACTG GATGTCCCTC TAGAGAGAAA CCACACACCC 
ATGGTGATCA ACAGTGATAC AGATGAGGAG GAGGAGGAGG AGGAGGAGGA GGTCTCAGCA 
GCACTCACTT TGGCCCATCT GAAAGAGAGA GGAATTGGTT TGTGGAGTAG AGACCCAGGC 
GCAGAAGAGG TCAAGTCCCA ACCACAGGTC CTTGTAGAAC AAAGCCAGAG TGCCTCTGGG 
AGAGACAGTG ACACGGATGT GGAGGAGGAA TCCTCAGGGC GAAAGAGAGA GATTATCCCA 
GACAGTCCCA TGGATGTAGA TGAGGCTCTT ACTGTCACAC AGCCAGAGAG CCAGCCTCCC 
CGTAGGCCCA ATGATGCAGA TGAGTACATG GATATGAGCT CCCCTGGCAG CCATCTAGTG 
GTAAATCAGG CCTCCTTTGC TGTGGTAGGC AAGACCAGAG CACAAGTGGA GGAGGAAGTC 
CCAGGGCCAT CTGTTATCCT GGGGGAGAAG CACCAGGTGC CTCTAGAGGG AGCCCAGCCT 
CCTGAGGAAG CCTGGGAAAC AGCTGTTCAG GAAGGCTCAT CCTCACCAGA GGCAGCAGCC 
TCTGTAAGGC CTAGCCAGCA GCCAGTAGCA GAAGATGCTG GGACAGAGTG TGCTACAGCT 
GTTTCTGAGC AGGAAAGTAC TCTTGAGGTG AGGTCCCAAA GCGGGTCACC TGCAGCACCA 
GTGGAGCAAG TGGTGATACA TACAGATACT TCAGGGGATC CCACCCTGCC ACAGAGAGAG 
GGAGCCCAAA CCCCCACAGG AAGGGAGAGA GAAGCACATG TGGGCAGGAC CAAGAGTGCC 
AAAGAGTGCT GTGATGCAGA ACCTGAAGAT CTGTGCCTTC CAGCTACCCA GTGCTTTGTG 
GAAGGGGAGA GCCAGCACCC AGAAGCTGTC CAGAGTTTGG AAAATGAGCC TACCCAACTC 
TTCCCATGTA CTCTTCCACA AGAGCCAGGA CCTTCCCATC TCAGCCTTCA GACTCCAGGT 
GCAGATACCT TGGATGTGCC TTGGGAAGTC TTGGCTACAC AGCCGTTCTG TCTAAGAGAA 
CAGTCAGAGA CTTCTGAGCT TCATGAAGCT CATGGATCTC AACCATCTCT GCCTAGGGAA 
CCACCAGGAC ACCAACATCT GGTTCATACC AGCCCAGTTC ACACAGAGCT GTTGAGAATT 
GAAGGCAGAG AGATACAGAC TGTGGAGAAA GCCATGGGTA TACCAAAGGA AATGGCAGAC 
AGGATGACCC CTGAGAGAGA GCCATTGGAG AGGGAAATCA GGGGAAGAAC AGAAAACAGT 
GAGAGAGATG TGATAGGAGA AGAACTAATT CAGGGGACAA AGGACAGAGA GCCAAAGAAG 
GTGTTAGCTA GAGACAGTCA GAGAAAAGAG GCTGACAAGG ATCTTGAAGG GAATAGGGAG 
AGTTTGGAGG TAGAAATAGA GATGTCCAAA GACTCACAGA AGAGAGAGAG AAAAGTAGAG 
AAACCAGAAC CAAAGAGAGA ATGGGAGCCA GCTGATTTAG AAGTGACTCC AGATAGAGGG 
GTGACAGAGG AAGGGAGCCA TGATCAGAAA GGGCAAATTG CTAGTCTAAC ACTAAAGCCT 
GGAGTTGGGG TGAAGGACCT TGAGGGACTT GCATCAGCCC CAATAATCAC AGGGAGCCAG 
GCAGATGGAG GAAAGGGAGA CCCTTTGAGC CCTGGGAGGC AGCAGAGAGG CCGCTTGAGT 
TGCCAGACGA CACCTGCTGG AAAGGCTTCC AGGGGTGATC CAGAGCCCCC AGACCACTGC 
CTGTTTTCTT CAGTGCCTGA AGCTTCAACT CAGAGTCTCC TCACCTCTCA GAGCCAAAAG 
CAGTCTACAC CTCAGCCTCT GTTTTCGACC TCTTCTTCTG AGATACCCCT TCCTGAGAGT 
CTTCACACAA AGCCTAATGT CAGGCCACGG CGGTCCTCCA GGATGACCCC CTCCCCGCAT 
TCCTCTGCTG CCCTTAAGCC AAATACTACC TGCCCCACAA ACCAGCCTGC TGCCTCTAGA 
CCAACATCCC GACCCACTCG GGGCAGGGCA AATAGGTCCT CTACCAGGAC CCCAGAACTG 
ATTGTCCCTG TAGACCCTGA ACTCCAGCCT TCCACCTCCA CAGAACAGCC TGTCATCCCA 
AAACTTACGT CCCAGGTCAC TGAGGGCAGG GTACAGATGC CTGAACCACT TCTCACAGGT 
CCCGAGATCC AGTCTCCCAC CTCCACAGAA CAGTCTGTCA CCCCAGACCG CAAACCTCGG 
GCCACTCGGG GCAGGCCAAG TAAATCTCCC AACAAGACCC CAGAACCACT TATTTCTACT 
GGCCCTGAAC TCCAACCTCC CACCTCCATA GAACAACCTG TCATACCTAA ACCCACTTCT 
CGGGTCACTC GGGGCAGACC ACGTAAGTCT TCTGTCAGGA CCCCAGAATC AGTTGTCTCC 
ACTGGCCCTG AACTTCAGCC TCTCACCTCT ATAGAACAGC CTGTTATCCC TGAACCTAGG 
GCCACTCGAG GTAGACCAAG TAAGTCTTCT ATCAAGACCC CAGAATCAGT TGTCCCCACT 
GGTCCTGAGC TGCAGCCTCT TACCTCTGCA AAACAGCCTG TCACCCCTAA CCTCACATCT 
CGGGCCTCTC GGGGTAGATC AAGTAAGTCT ATCAGGACCC CGGAACCAGT TGTCCAAACT 
GGCCCTGAAT TCCATCCATC CACCTCCACA GAACAGCCTG ATACCCGTGA GCCCTCATCT 
CAGGCTAGGA CACGTAGGTC TGCTGTCAAG ACTCCTGAAG CGAGTGTCCC CACAACCCCT 
GAACTCCAGC CTTTCACTTC TAAAAAACAG CCTGCCCCTA AGCCTACAGC TCTGGTCACT 
CAGGGAAGGA CATATAAACC CTCCACTGAA GATTGTGAAT CAGTTGGGCC AGTGGCCCCT 
GATTTTGAGC CTTCCACCTC TACAGACCAT CTTGTCACCC CTAAGGTTAC AGATCAGAGC 
TTAACACTAC AGTCTTCACC CCTAAGTGCT TCCCCAGTTT CCAGTACCCC TGATCTCAAG 
CCTCCTGTCC CCATAGCCCA GCCTGTTACC CCAGAGCCCA TTCCACAAGC CAATCACCAA 
AGGAAACGAA GGGCTGCTGG GAAGCAGGGT TCCCGCACAG TCCCTCTGGG TCATAAGTCT 
TACTCTGCCC TCTCTGAACC TGAGCCCCAA TCTTCAGCCA GCCAAAGCTC AGGAGCATCA 
GAAGCAGACT CACCCCGTCA AAAACGTCCC AGAAGACAAG CCTCCCAGAA AACTGTAGTC 
ATCAAGGAAG AACCTGTAGA AACAGAAGTG AAGGAAGAGC CTCAAGAGAC TGCAATTCCA 
ACACCAGAAA AGAGAAAGAG GGACCATGCA GAAGAAGTGA CCCAGGGAAA ACCAACTCGA 
AGCCGGCGAA CTAAACCTAA CCAAGAAACA GCCCCCAAGG TACTGTTCAC AGGAGTCATG 
GATTCTCGTG GCGAGCGTGC AGTGCTGGCT CTGGGAGGCA GTCTGGCCAG CTCAGTAAAT 
GAGGCCTCCC ACTTAGTTAC TGATCGCATC CGCAGGACAG TCAAGTTCTT GTGTGCCCTG 
GGGAAGGGAA TTCCCATCCT GTCCCTGAAC TGGCTGTACC AGTCCCGAAA GGCTGGTTGC 
TTCTTGCCGC CTGATGACTA CTTAGTGACT GATCCTGAGC AAGAGAAGAA CTTTAGCTTC 
AGCCTTCGGG ATTCCCTGTG CCGGGCTCGG GAACGAAGAC TGCTGGAGGA CTATGAGATT 
CATGTGACCC CTGGCGTGCA GCCACCCCCC CCTCAGATGG GCGAGATCAT CAGTTGCTGT 
GGAGGCACTT TCCTGCCCAG CATGCCTCAC TCCTATAAGC TTCACCGAGT CATCATAACT 
TGCACTGAAG ACCTACCACG CTGTGCTATT CCATCCCGCC TGGGGCTACC CCTCCTCTCT 
CCTGAGTTCC TCCTGACTGG AGTGCTGAAG CAGGAAGCCA CACCAGAGGC CTTTGTTCTC 
TCCAATTTGG AAATGTCATC TACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mdc1 ( XM_006524262.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524325.1 Mus musculus mediator of DNA damage checkpoint 1 (Mdc1), transcript variant X3, mRNA.

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CloneID	OMu53578
Clone ID Related Accession (Same CDS sequence)	XM_006524262.3 , XM_006524262.4 , XM_030249799.1
Accession Version	XM_006524262.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5124bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	mediator of DNA damage checkpoint protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006524262.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGCA CCCAGGTGAT TGACTGGGAT GCTGAAGAAG AGGAAGAGAC AGAACTATCC 
AGTGGCTCCT TGGGGTATAG TGTGGAGCCT ATAGGGCAGC TACGTCTCTT CAGTGGAACT 
CATGGACCAG AAAGAGATTT CCCCCTCTAC CTTGGCAAGA ATGTAGTTGG TCGAAGCCCT 
GACTGCTCTG TGGCCCTGCC TTTTCCATCC ATCTCCAAAC AGCATGCAGT AATTGAAATC 
TCAGCTTGGA ACAAAGCCCC TATCCTCCAG GATTGTGGGA GCCTCAATGG CACTCAAATC 
GTAAAGCCTC CTAGGGTCCT ACCTCCTGGA GTGAGCCATC GTCTGAGGGA CCAGGAATTA 
ATTCTGTTTG CAGATTTTCC CTGCCAGTAC CATCGCCTGG ATGTCCCACC ACCCTTGGTC 
CCTCGGAGTC TTCTAACTAT AGAGAAAACC CCCAGAATAC GGATAGAATC CCAAAATTCC 
AGAGTTTTGT TGGCTGCCGA TTCAGAGGAA GAAGGGGATT TTCCTTCTGG AAGGTGTGTG 
GCGAATGGAC AAAGGAATAC AGCATCCCCT TCAGCAACAG TAGTTCCAGA AAGTGATGAA 
GAGGTCTCTT CTCCGGCCCC AAGTGTCCCC GGGCCATCTT CACCCTTTGG TTTGGGCAGT 
GACACTGATG AAGAACAAGG TCAGCAACCA GGAGTAGAGG AGTCCTCTTT AGCTGACAGC 
AGTGGTGCTG CAGGGGAAGC TGAGCAGCCT GAAGCTAACG GAACGACAGC TGGCATACAG 
GCTCAGCCTA CTGAGCACAA ATTGAAGGAC ACAAAAGTCA AGAAGGAGGC AGGCAGGGCA 
GGGGTCTCAG ATGGGTCAGT TCTGGAGAGG AGCCCCACTC TTGGAGAGGA CAGTGACACA 
GAAGTGGACG AAGATCACAA ACCTGGGTTT GCGGACAGTG AGACAGATGT GGAAGAAGAG 
AGGATCCCTG TGACCCCCCC TGTAGCCCCT GTGAAGAAAA ACCAAGTGCT ACTTGCAGTT 
GGTATAGGAG ATCCTGAAGC ACCTGGTGTG GCACATCTGC AGGACTGCCT AGCTGGTAGT 
GGCACAGATG TGGAGGACAA GACTGCACTG GATGTCCCTC TAGAGAGAAA CCACACACCC 
ATGGTGATCA ACAGTGATAC AGATGAGGAG GAGGAGGAGG AGGAGGAGGA GGTCTCAGCA 
GCACTCACTT TGGCCCATCT GAAAGAGAGA GGAATTGGTT TGTGGAGTAG AGACCCAGGC 
GCAGAAGAGG TCAAGTCCCA ACCACAGGTC CTTGTAGAAC AAAGCCAGAG TGCCTCTGGG 
AGAGACAGTG ACACGGATGT GGAGGAGGAA TCCTCAGGGC GAAAGAGAGA GATTATCCCA 
GACAGTCCCA TGGATGTAGA TGAGGCTCTT ACTGTCACAC AGCCAGAGAG CCAGCCTCCC 
CGTAGGCCCA ATGATGCAGA TGAGTACATG GATATGAGCT CCCCTGGCAG CCATCTAGTG 
GTAAATCAGG CCTCCTTTGC TGTGGTAGGC AAGACCAGAG CACAAGTGGA GGAGGAAGTC 
CCAGGGCCAT CTGTTATCCT GGGGGAGAAG CACCAGGTGC CTCTAGAGGG AGCCCAGCCT 
CCTGAGGAAG CCTGGGAAAC AGCTGTTCAG GAAGGCTCAT CCTCACCAGA GGCAGCAGCC 
TCTGTAAGGC CTAGCCAGCA GCCAGTAGCA GAAGATGCTG GGACAGAGTG TGCTACAGCT 
GTTTCTGAGC AGGAAAGTAC TCTTGAGGTG AGGTCCCAAA GCGGGTCACC TGCAGCACCA 
GTGGAGCAAG TGGTGATACA TACAGATACT TCAGGGGATC CCACCCTGCC ACAGAGAGAG 
GGAGCCCAAA CCCCCACAGG AAGGGAGAGA GAAGCACATG TGGGCAGGAC CAAGAGTGCC 
AAAGAGTGCT GTGATGAACC TGAAGATCTG TGCCTTCCAG CTACCCAGTG CTTTGTGGAA 
GGGGAGAGCC AGCACCCAGA AGCTGTCCAG AGTTTGGAAA ATGAGCCTAC CCAACTCTTC 
CCATGTACTC TTCCACAAGA GCCAGGACCT TCCCATCTCA GCCTTCAGAC TCCAGGTGCA 
GATACCTTGG ATGTGCCTTG GGAAGTCTTG GCTACACAGC CGTTCTGTCT AAGAGAACAG 
TCAGAGACTT CTGAGCTTCA TGAAGCTCAT GGATCTCAAC CATCTCTGCC TAGGGAACCA 
CCAGGACACC AACATCTGGT TCATACCAGC CCAGTTCACA CAGAGCTGTT GAGAATTGAA 
GGCAGAGAGA TACAGACTGT GGAGAAAGCC ATGGGTATAC CAAAGGAAAT GGCAGACAGG 
ATGACCCCTG AGAGAGAGCC ATTGGAGAGG GAAATCAGGG GAAGAACAGA AAACAGTGAG 
AGAGATGTGA TAGGAGAAGA ACTAATTCAG GGGACAAAGG ACAGAGAGCC AAAGAAGGTG 
TTAGCTAGAG ACAGTCAGAG AAAAGAGGCT GACAAGGATC TTGAAGGGAA TAGGGAGAGT 
TTGGAGGTAG AAATAGAGAT GTCCAAAGAC TCACAGAAGA GAGAGAGAAA AGTAGAGAAA 
CCAGAACCAA AGAGAGAATG GGAGCCAGCT GATTTAGAAG TGACTCCAGA TAGAGGGGTG 
ACAGAGGAAG GGAGCCATGA TCAGAAAGGG CAAATTGCTA GTCTAACACT AAAGCCTGGA 
GTTGGGGTGA AGGACCTTGA GGGACTTGCA TCAGCCCCAA TAATCACAGG GAGCCAGGCA 
GATGGAGGAA AGGGAGACCC TTTGAGCCCT GGGAGGCAGC AGAGAGGCCG CTTGAGTTGC 
CAGACGACAC CTGCTGGAAA GGCTTCCAGG GGTGATCCAG AGCCCCCAGA CCACTGCCTG 
TTTTCTTCAG TGCCTGAAGC TTCAACTCAG AGTCTCCTCA CCTCTCAGAG CCAAAAGCAG 
TCTACACCTC AGCCTCTGTT TTCGACCTCT TCTTCTGAGA TACCCCTTCC TGAGAGTCTT 
CACACAAAGC CTAATGTCAG GCCACGGCGG TCCTCCAGGA TGACCCCCTC CCCGCATTCC 
TCTGCTGCCC TTAAGCCAAA TACTACCTGC CCCACAAACC AGCCTGCTGC CTCTAGACCA 
ACATCCCGAC CCACTCGGGG CAGGGCAAAT AGGTCCTCTA CCAGGACCCC AGAACTGATT 
GTCCCTGTAG ACCCTGAACT CCAGCCTTCC ACCTCCACAG AACAGCCTGT CATCCCAAAA 
CTTACGTCCC AGGTCACTGA GGGCAGGGTA CAGATGCCTG AACCACTTCT CACAGGTCCC 
GAGATCCAGT CTCCCACCTC CACAGAACAG TCTGTCACCC CAGACCGCAA ACCTCGGGCC 
ACTCGGGGCA GGCCAAGTAA ATCTCCCAAC AAGACCCCAG AACCACTTAT TTCTACTGGC 
CCTGAACTCC AACCTCCCAC CTCCATAGAA CAACCTGTCA TACCTAAACC CACTTCTCGG 
GTCACTCGGG GCAGACCACG TAAGTCTTCT GTCAGGACCC CAGAATCAGT TGTCTCCACT 
GGCCCTGAAC TTCAGCCTCT CACCTCTATA GAACAGCCTG TTATCCCTGA ACCTAGGGCC 
ACTCGAGGTA GACCAAGTAA GTCTTCTATC AAGACCCCAG AATCAGTTGT CCCCACTGGT 
CCTGAGCTGC AGCCTCTTAC CTCTGCAAAA CAGCCTGTCA CCCCTAACCT CACATCTCGG 
GCCTCTCGGG GTAGATCAAG TAAGTCTATC AGGACCCCGG AACCAGTTGT CCAAACTGGC 
CCTGAATTCC ATCCATCCAC CTCCACAGAA CAGCCTGATA CCCGTGAGCC CTCATCTCAG 
GCTAGGACAC GTAGGTCTGC TGTCAAGACT CCTGAAGCGA GTGTCCCCAC AACCCCTGAA 
CTCCAGCCTT TCACTTCTAA AAAACAGCCT GCCCCTAAGC CTACAGCTCT GGTCACTCAG 
GGAAGGACAT ATAAACCCTC CACTGAAGAT TGTGAATCAG TTGGGCCAGT GGCCCCTGAT 
TTTGAGCCTT CCACCTCTAC AGACCATCTT GTCACCCCTA AGGTTACAGA TCAGAGCTTA 
ACACTACAGT CTTCACCCCT AAGTGCTTCC CCAGTTTCCA GTACCCCTGA TCTCAAGCCT 
CCTGTCCCCA TAGCCCAGCC TGTTACCCCA GAGCCCATTC CACAAGCCAA TCACCAAAGG 
AAACGAAGGG CTGCTGGGAA GCAGGGTTCC CGCACAGTCC CTCTGGGTCA TAAGTCTTAC 
TCTGCCCTCT CTGAACCTGA GCCCCAATCT TCAGCCAGCC AAAGCTCAGG AGCATCAGAA 
GCAGACTCAC CCCGTCAAAA ACGTCCCAGA AGACAAGCCT CCCAGAAAAC TGTAGTCATC 
AAGGAAGAAC CTGTAGAAAC AGAAGTGAAG GAAGAGCCTC AAGAGACTGC AATTCCAACA 
CCAGAAAAGA GAAAGAGGGA CCATGCAGAA GAAGTGACCC AGGGAAAACC AACTCGAAGC 
CGGCGAACTA AACCTAACCA AGAAACAGCC CCCAAGGTAC TGTTCACAGG AGTCATGGAT 
TCTCGTGGCG AGCGTGCAGT GCTGGCTCTG GGAGGCAGTC TGGCCAGCTC AGTAAATGAG 
GCCTCCCACT TAGTTACTGA TCGCATCCGC AGGACAGTCA AGTTCTTGTG TGCCCTGGGG 
AAGGGAATTC CCATCCTGTC CCTGAACTGG CTGTACCAGT CCCGAAAGGC TGGTTGCTTC 
TTGCCGCCTG ATGACTACTT AGTGACTGAT CCTGAGCAAG AGAAGAACTT TAGCTTCAGC 
CTTCGGGATT CCCTGTGCCG GGCTCGGGAA CGAAGACTGC TGGAGGACTA TGAGATTCAT 
GTGACCCCTG GCGTGCAGCC ACCCCCCCCT CAGATGGGCG AGATCATCAG TTGCTGTGGA 
GGCACTTTCC TGCCCAGCAT GCCTCACTCC TATAAGCTTC ACCGAGTCAT CATAACTTGC 
ACTGAAGACC TACCACGCTG TGCTATTCCA TCCCGCCTGG GGCTACCCCT CCTCTCTCCT 
GAGTTCCTCC TGACTGGAGT GCTGAAGCAG GAAGCCACAC CAGAGGCCTT TGTTCTCTCC 
AATTTGGAAA TGTCATCTAC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524325.1
CDS	169..5292
Translation	MESTQVIDWDAEEEEETELSSGSLGYSVEPIGQLRLFSGTHGPERDFPLYLGKNVVGRSPDCSVALPFPSISKQHAVIEISAWNKAPILQDCGSLNGTQIVKPPRVLPPGVSHRLRDQELILFADFPCQYHRLDVPPPLVPRSLLTIEKTPRIRIESQNSRVLLAADSEEEGDFPSGRCVANGQRNTASPSATVVPESDEEVSSPAPSVPGPSSPFGLGSDTDEEQGQQPGVEESSLADSSGAAGEAEQPEANGTTAGIQAQPTEHKLKDTKVKKEAGRAGVSDGSVLERSPTLGEDSDTEVDEDHKPGFADSETDVEEERIPVTPPVAPVKKNQVLLAVGIGDPEAPGVAHLQDCLAGSGTDVEDKTALDVPLERNHTPMVINSDTDEEEEEEEEEVSAALTLAHLKERGIGLWSRDPGAEEVKSQPQVLVEQSQSASGRDSDTDVEEESSGRKREIIPDSPMDVDEALTVTQPESQPPRRPNDADEYMDMSSPGSHLVVNQASFAVVGKTRAQVEEEVPGPSVILGEKHQVPLEGAQPPEEAWETAVQEGSSSPEAAASVRPSQQPVAEDAGTECATAVSEQESTLEVRSQSGSPAAPVEQVVIHTDTSGDPTLPQREGAQTPTGREREAHVGRTKSAKECCDEPEDLCLPATQCFVEGESQHPEAVQSLENEPTQLFPCTLPQEPGPSHLSLQTPGADTLDVPWEVLATQPFCLREQSETSELHEAHGSQPSLPREPPGHQHLVHTSPVHTELLRIEGREIQTVEKAMGIPKEMADRMTPEREPLEREIRGRTENSERDVIGEELIQGTKDREPKKVLARDSQRKEADKDLEGNRESLEVEIEMSKDSQKRERKVEKPEPKREWEPADLEVTPDRGVTEEGSHDQKGQIASLTLKPGVGVKDLEGLASAPIITGSQADGGKGDPLSPGRQQRGRLSCQTTPAGKASRGDPEPPDHCLFSSVPEASTQSLLTSQSQKQSTPQPLFSTSSSEIPLPESLHTKPNVRPRRSSRMTPSPHSSAALKPNTTCPTNQPAASRPTSRPTRGRANRSSTRTPELIVPVDPELQPSTSTEQPVIPKLTSQVTEGRVQMPEPLLTGPEIQSPTSTEQSVTPDRKPRATRGRPSKSPNKTPEPLISTGPELQPPTSIEQPVIPKPTSRVTRGRPRKSSVRTPESVVSTGPELQPLTSIEQPVIPEPRATRGRPSKSSIKTPESVVPTGPELQPLTSAKQPVTPNLTSRASRGRSSKSIRTPEPVVQTGPEFHPSTSTEQPDTREPSSQARTRRSAVKTPEASVPTTPELQPFTSKKQPAPKPTALVTQGRTYKPSTEDCESVGPVAPDFEPSTSTDHLVTPKVTDQSLTLQSSPLSASPVSSTPDLKPPVPIAQPVTPEPIPQANHQRKRRAAGKQGSRTVPLGHKSYSALSEPEPQSSASQSSGASEADSPRQKRPRRQASQKTVVIKEEPVETEVKEEPQETAIPTPEKRKRDHAEEVTQGKPTRSRRTKPNQETAPKVLFTGVMDSRGERAVLALGGSLASSVNEASHLVTDRIRRTVKFLCALGKGIPILSLNWLYQSRKAGCFLPPDDYLVTDPEQEKNFSFSLRDSLCRARERRLLEDYEIHVTPGVQPPPPQMGEIISCCGGTFLPSMPHSYKLHRVIITCTEDLPRCAIPSRLGLPLLSPEFLLTGVLKQEATPEAFVLSNLEMSST

Target ORF information:

RefSeq Version	XM_006524262.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus mediator of DNA damage checkpoint 1 (Mdc1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524262.3

ORF Insert Sequence:

ATGGAAAGCA CCCAGGTGAT TGACTGGGAT GCTGAAGAAG AGGAAGAGAC AGAACTATCC 
AGTGGCTCCT TGGGGTATAG TGTGGAGCCT ATAGGGCAGC TACGTCTCTT CAGTGGAACT 
CATGGACCAG AAAGAGATTT CCCCCTCTAC CTTGGCAAGA ATGTAGTTGG TCGAAGCCCT 
GACTGCTCTG TGGCCCTGCC TTTTCCATCC ATCTCCAAAC AGCATGCAGT AATTGAAATC 
TCAGCTTGGA ACAAAGCCCC TATCCTCCAG GATTGTGGGA GCCTCAATGG CACTCAAATC 
GTAAAGCCTC CTAGGGTCCT ACCTCCTGGA GTGAGCCATC GTCTGAGGGA CCAGGAATTA 
ATTCTGTTTG CAGATTTTCC CTGCCAGTAC CATCGCCTGG ATGTCCCACC ACCCTTGGTC 
CCTCGGAGTC TTCTAACTAT AGAGAAAACC CCCAGAATAC GGATAGAATC CCAAAATTCC 
AGAGTTTTGT TGGCTGCCGA TTCAGAGGAA GAAGGGGATT TTCCTTCTGG AAGGTGTGTG 
GCGAATGGAC AAAGGAATAC AGCATCCCCT TCAGCAACAG TAGTTCCAGA AAGTGATGAA 
GAGGTCTCTT CTCCGGCCCC AAGTGTCCCC GGGCCATCTT CACCCTTTGG TTTGGGCAGT 
GACACTGATG AAGAACAAGG TCAGCAACCA GGAGTAGAGG AGTCCTCTTT AGCTGACAGC 
AGTGGTGCTG CAGGGGAAGC TGAGCAGCCT GAAGCTAACG GAACGACAGC TGGCATACAG 
GCTCAGCCTA CTGAGCACAA ATTGAAGGAC ACAAAAGTCA AGAAGGAGGC AGGCAGGGCA 
GGGGTCTCAG ATGGGTCAGT TCTGGAGAGG AGCCCCACTC TTGGAGAGGA CAGTGACACA 
GAAGTGGACG AAGATCACAA ACCTGGGTTT GCGGACAGTG AGACAGATGT GGAAGAAGAG 
AGGATCCCTG TGACCCCCCC TGTAGCCCCT GTGAAGAAAA ACCAAGTGCT ACTTGCAGTT 
GGTATAGGAG ATCCTGAAGC ACCTGGTGTG GCACATCTGC AGGACTGCCT AGCTGGTAGT 
GGCACAGATG TGGAGGACAA GACTGCACTG GATGTCCCTC TAGAGAGAAA CCACACACCC 
ATGGTGATCA ACAGTGATAC AGATGAGGAG GAGGAGGAGG AGGAGGAGGA GGTCTCAGCA 
GCACTCACTT TGGCCCATCT GAAAGAGAGA GGAATTGGTT TGTGGAGTAG AGACCCAGGC 
GCAGAAGAGG TCAAGTCCCA ACCACAGGTC CTTGTAGAAC AAAGCCAGAG TGCCTCTGGG 
AGAGACAGTG ACACGGATGT GGAGGAGGAA TCCTCAGGGC GAAAGAGAGA GATTATCCCA 
GACAGTCCCA TGGATGTAGA TGAGGCTCTT ACTGTCACAC AGCCAGAGAG CCAGCCTCCC 
CGTAGGCCCA ATGATGCAGA TGAGTACATG GATATGAGCT CCCCTGGCAG CCATCTAGTG 
GTAAATCAGG CCTCCTTTGC TGTGGTAGGC AAGACCAGAG CACAAGTGGA GGAGGAAGTC 
CCAGGGCCAT CTGTTATCCT GGGGGAGAAG CACCAGGTGC CTCTAGAGGG AGCCCAGCCT 
CCTGAGGAAG CCTGGGAAAC AGCTGTTCAG GAAGGCTCAT CCTCACCAGA GGCAGCAGCC 
TCTGTAAGGC CTAGCCAGCA GCCAGTAGCA GAAGATGCTG GGACAGAGTG TGCTACAGCT 
GTTTCTGAGC AGGAAAGTAC TCTTGAGGTG AGGTCCCAAA GCGGGTCACC TGCAGCACCA 
GTGGAGCAAG TGGTGATACA TACAGATACT TCAGGGGATC CCACCCTGCC ACAGAGAGAG 
GGAGCCCAAA CCCCCACAGG AAGGGAGAGA GAAGCACATG TGGGCAGGAC CAAGAGTGCC 
AAAGAGTGCT GTGATGAACC TGAAGATCTG TGCCTTCCAG CTACCCAGTG CTTTGTGGAA 
GGGGAGAGCC AGCACCCAGA AGCTGTCCAG AGTTTGGAAA ATGAGCCTAC CCAACTCTTC 
CCATGTACTC TTCCACAAGA GCCAGGACCT TCCCATCTCA GCCTTCAGAC TCCAGGTGCA 
GATACCTTGG ATGTGCCTTG GGAAGTCTTG GCTACACAGC CGTTCTGTCT AAGAGAACAG 
TCAGAGACTT CTGAGCTTCA TGAAGCTCAT GGATCTCAAC CATCTCTGCC TAGGGAACCA 
CCAGGACACC AACATCTGGT TCATACCAGC CCAGTTCACA CAGAGCTGTT GAGAATTGAA 
GGCAGAGAGA TACAGACTGT GGAGAAAGCC ATGGGTATAC CAAAGGAAAT GGCAGACAGG 
ATGACCCCTG AGAGAGAGCC ATTGGAGAGG GAAATCAGGG GAAGAACAGA AAACAGTGAG 
AGAGATGTGA TAGGAGAAGA ACTAATTCAG GGGACAAAGG ACAGAGAGCC AAAGAAGGTG 
TTAGCTAGAG ACAGTCAGAG AAAAGAGGCT GACAAGGATC TTGAAGGGAA TAGGGAGAGT 
TTGGAGGTAG AAATAGAGAT GTCCAAAGAC TCACAGAAGA GAGAGAGAAA AGTAGAGAAA 
CCAGAACCAA AGAGAGAATG GGAGCCAGCT GATTTAGAAG TGACTCCAGA TAGAGGGGTG 
ACAGAGGAAG GGAGCCATGA TCAGAAAGGG CAAATTGCTA GTCTAACACT AAAGCCTGGA 
GTTGGGGTGA AGGACCTTGA GGGACTTGCA TCAGCCCCAA TAATCACAGG GAGCCAGGCA 
GATGGAGGAA AGGGAGACCC TTTGAGCCCT GGGAGGCAGC AGAGAGGCCG CTTGAGTTGC 
CAGACGACAC CTGCTGGAAA GGCTTCCAGG GGTGATCCAG AGCCCCCAGA CCACTGCCTG 
TTTTCTTCAG TGCCTGAAGC TTCAACTCAG AGTCTCCTCA CCTCTCAGAG CCAAAAGCAG 
TCTACACCTC AGCCTCTGTT TTCGACCTCT TCTTCTGAGA TACCCCTTCC TGAGAGTCTT 
CACACAAAGC CTAATGTCAG GCCACGGCGG TCCTCCAGGA TGACCCCCTC CCCGCATTCC 
TCTGCTGCCC TTAAGCCAAA TACTACCTGC CCCACAAACC AGCCTGCTGC CTCTAGACCA 
ACATCCCGAC CCACTCGGGG CAGGGCAAAT AGGTCCTCTA CCAGGACCCC AGAACTGATT 
GTCCCTGTAG ACCCTGAACT CCAGCCTTCC ACCTCCACAG AACAGCCTGT CATCCCAAAA 
CTTACGTCCC AGGTCACTGA GGGCAGGGTA CAGATGCCTG AACCACTTCT CACAGGTCCC 
GAGATCCAGT CTCCCACCTC CACAGAACAG TCTGTCACCC CAGACCGCAA ACCTCGGGCC 
ACTCGGGGCA GGCCAAGTAA ATCTCCCAAC AAGACCCCAG AACCACTTAT TTCTACTGGC 
CCTGAACTCC AACCTCCCAC CTCCATAGAA CAACCTGTCA TACCTAAACC CACTTCTCGG 
GTCACTCGGG GCAGACCACG TAAGTCTTCT GTCAGGACCC CAGAATCAGT TGTCTCCACT 
GGCCCTGAAC TTCAGCCTCT CACCTCTATA GAACAGCCTG TTATCCCTGA ACCTAGGGCC 
ACTCGAGGTA GACCAAGTAA GTCTTCTATC AAGACCCCAG AATCAGTTGT CCCCACTGGT 
CCTGAGCTGC AGCCTCTTAC CTCTGCAAAA CAGCCTGTCA CCCCTAACCT CACATCTCGG 
GCCTCTCGGG GTAGATCAAG TAAGTCTATC AGGACCCCGG AACCAGTTGT CCAAACTGGC 
CCTGAATTCC ATCCATCCAC CTCCACAGAA CAGCCTGATA CCCGTGAGCC CTCATCTCAG 
GCTAGGACAC GTAGGTCTGC TGTCAAGACT CCTGAAGCGA GTGTCCCCAC AACCCCTGAA 
CTCCAGCCTT TCACTTCTAA AAAACAGCCT GCCCCTAAGC CTACAGCTCT GGTCACTCAG 
GGAAGGACAT ATAAACCCTC CACTGAAGAT TGTGAATCAG TTGGGCCAGT GGCCCCTGAT 
TTTGAGCCTT CCACCTCTAC AGACCATCTT GTCACCCCTA AGGTTACAGA TCAGAGCTTA 
ACACTACAGT CTTCACCCCT AAGTGCTTCC CCAGTTTCCA GTACCCCTGA TCTCAAGCCT 
CCTGTCCCCA TAGCCCAGCC TGTTACCCCA GAGCCCATTC CACAAGCCAA TCACCAAAGG 
AAACGAAGGG CTGCTGGGAA GCAGGGTTCC CGCACAGTCC CTCTGGGTCA TAAGTCTTAC 
TCTGCCCTCT CTGAACCTGA GCCCCAATCT TCAGCCAGCC AAAGCTCAGG AGCATCAGAA 
GCAGACTCAC CCCGTCAAAA ACGTCCCAGA AGACAAGCCT CCCAGAAAAC TGTAGTCATC 
AAGGAAGAAC CTGTAGAAAC AGAAGTGAAG GAAGAGCCTC AAGAGACTGC AATTCCAACA 
CCAGAAAAGA GAAAGAGGGA CCATGCAGAA GAAGTGACCC AGGGAAAACC AACTCGAAGC 
CGGCGAACTA AACCTAACCA AGAAACAGCC CCCAAGGTAC TGTTCACAGG AGTCATGGAT 
TCTCGTGGCG AGCGTGCAGT GCTGGCTCTG GGAGGCAGTC TGGCCAGCTC AGTAAATGAG 
GCCTCCCACT TAGTTACTGA TCGCATCCGC AGGACAGTCA AGTTCTTGTG TGCCCTGGGG 
AAGGGAATTC CCATCCTGTC CCTGAACTGG CTGTACCAGT CCCGAAAGGC TGGTTGCTTC 
TTGCCGCCTG ATGACTACTT AGTGACTGAT CCTGAGCAAG AGAAGAACTT TAGCTTCAGC 
CTTCGGGATT CCCTGTGCCG GGCTCGGGAA CGAAGACTGC TGGAGGACTA TGAGATTCAT 
GTGACCCCTG GCGTGCAGCC ACCCCCCCCT CAGATGGGCG AGATCATCAG TTGCTGTGGA 
GGCACTTTCC TGCCCAGCAT GCCTCACTCC TATAAGCTTC ACCGAGTCAT CATAACTTGC 
ACTGAAGACC TACCACGCTG TGCTATTCCA TCCCGCCTGG GGCTACCCCT CCTCTCTCCT 
GAGTTCCTCC TGACTGGAGT GCTGAAGCAG GAAGCCACAC CAGAGGCCTT TGTTCTCTCC 
AATTTGGAAA TGTCATCTAC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mdc1 ( XM_006524263.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524326.1 Mus musculus mediator of DNA damage checkpoint 1 (Mdc1), transcript variant X4, mRNA.

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CloneID	OMu53579
Clone ID Related Accession (Same CDS sequence)	XM_006524263.3 , XM_006524263.4
Accession Version	XM_006524263.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	mediator of DNA damage checkpoint protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006524263.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGCA CCCAGGTGAT TGACTGGGAT GCTGAAGAAG AGGAAGAGAC AGAACTATCC 
AGTGGCTCCT TGGGGTATAG TGTGGAGCCT ATAGGGCAGC TACGTCTCTT CAGTGGAACT 
CATGGACCAG AAAGAGATTT CCCCCTCTAC CTTGGCAAGA ATGTAGTTGG TCGAAGCCCT 
GACTGCTCTG TGGCCCTGCC TTTTCCATCC ATCTCCAAAC AGCATGCAGT AATTGAAATC 
TCAGCTTGGA ACAAAGCCCC TATCCTCCAG GATTGTGGGA GCCTCAATGG CACTCAAATC 
GTAAAGCCTC CTAGGGTCCT ACCTCCTGGA GTGAGCCATC GTCTGAGGGA CCAGGAATTA 
ATTCTGTTTG CAGATTTTCC CTGCCAGTAC CATCGCCTGG ATGTCCCACC ACCCTTGGTC 
CCTCGGAGTC TTCTAACTAT AGAGAAAACC CCCAGAATAC GGATAGAATC CCAAAATTCC 
AGAGTTTTGT TGGCTGCCGA TTCAGAGGAA GAAGGGGATT TTCCTTCTGG AAGGTGTGTG 
GCGAATGGAC AAAGGAATAC AGCATCCCCT TCAGCAACAG TAGTTCCAGA AAGTGATGAA 
GAGGTCTCTT CTCCGGCCCC AAGTGTCCCC GGGCCATCTT CACCCTTTGG TTTGGGCAGT 
GACACTGATG AAGAACAAGG TCAGCAACCA GGAGTAGAGG AGTCCTCTTT AGCTGACAGC 
AGTGGTGCTG CAGGGGAAGC TGAGCAGCCT GAAGCTAACG GAACGACAGC TGGCATACAG 
GCTCAGCCTA CTGAGCACAA ATTGAAGGAC ACAAAAGTCA AGAAGGAGGC AGGCAGGGCA 
GGGGTCTCAG ATGGGTCAGT TCTGGAGAGG AGCCCCACTC TTGGAGAGGA CAGTGACACA 
GAAGTGGACG AAGATCACAA ACCTGGGTTT GCGGACAGTG AGACAGATGT GGAAGAAGAG 
AGGATCCCTG TGACCCCCCC TGTAGCCCCT GTGAAGAAAA ACCAAGTGCT ACTTGCAGTT 
GGTATAGGAG ATCCTGAAGC ACCTGGTGTG GCACATCTGC AGGACTGCCT AGCTGGTAGT 
GGCACAGATG TGGAGGACAA GACTGCACTG GATGTCCCTC TAGAGAGAAA CCACACACCC 
ATGGTGATCA ACAGTGATAC AGATGAGGAG GAGGAGGAGG AGGAGGAGGA GGTCTCAGCA 
GCACTCACTT TGGCCCATCT GAAAGAGAGA GGAATTGGTT TGTGGAGTAG AGACCCAGGC 
GCAGAAGAGG TCAAGTCCCA ACCACAGGTC CTTGTAGAAC AAAGCCAGAG TGCCTCTGGG 
AGAGACAGTG ACACGGATGT GGAGGAGGAA TCCTCAGGGC GAAAGAGAGA GATTATCCCA 
GACAGTCCCA TGGATGTAGA TGAGGCTCTT ACTGTCACAC AGCCAGAGAG CCAGCCTCCC 
CGTAGGCCCA ATGATGCAGA TGAGTACATG GATATGAGCT CCCCTGGCAG CCATCTAGTG 
GTAAATCAGG CCTCCTTTGC TGTGGTAGGC AAGACCAGAG CACAAGTGGA GGAGGAAGTC 
CCAGGGCCAT CTGTTATCCT GGGGGAGAAG CACCAGGTGC CTCTAGAGGG AGCCCAGCCT 
CCTGAGGAAG CCTGGGAAAC AGCTGTTCAG GAAGGCTCAT CCTCACCAGA GGCAGCAGCC 
TCTGTAAGGC CTAGCCAGCA GCCAGTAGCA GAAGATGCTG GGACAGAGTG TGCTACAGCT 
GTTTCTGAGC AGGAAAGTAC TCTTGAGGTG AGGTCCCAAA GCGGGTCACC TGCAGCACCA 
GTGGAGCAAG TGGTGATACA TACAGATACT TCAGGGGATC CCACCCTGCC ACAGAGAGAG 
GGAGCCCAAA CCCCCACAGG AAGGGAGAGA GAAGCACATG TGGGCAGGAC CAAGAGTGCC 
AAAGAGTGCT GTGATGCAGA ACCTGAAGAT CTGTGCCTTC CAGCTACCCA GTGCTTTGTG 
GAAGGGGAGA GCCAGCACCC AGAAGCTGTC CAGAGTTTGG AAAATGAGCC TACCCAACTC 
TTCCCATGTA CTCTTCCACA AGAGCCAGGA CCTTCCCATC TCAGCCTTCA GACTCCAGGT 
GCAGATACCT TGGATGTGCC TTGGGAAGTC TTGGCTACAC AGCCGTTCTG TCTAAGAGAA 
CAGTCAGAGA CTTCTGAGCT TCATGAAGCT CATGGATCTC AACCATCTCT GCCTAGGGAA 
CCACCAGGAC ACCAACATCT GGTTCATACC AGCCCAGTTC ACACAGAGCT GTTGAGAATT 
GAAGGCAGAG AGATACAGAC TGTGGAGAAA GCCATGGGCC GCTTGAGTTG CCAGACGACA 
CCTGCTGGAA AGGCTTCCAG GGGTGATCCA GAGCCCCCAG ACCACTGCCT GTTTTCTTCA 
GTGCCTGAAG CTTCAACTCA GAGTCTCCTC ACCTCTCAGA GCCAAAAGCA GTCTACACCT 
CAGCCTCTGT TTTCGACCTC TTCTTCTGAG ATACCCCTTC CTGAGAGTCT TCACACAAAG 
CCTAATGTCA GGCCACGGCG GTCCTCCAGG ATGACCCCCT CCCCGCATTC CTCTGCTGCC 
CTTAAGCCAA ATACTACCTG CCCCACAAAC CAGCCTGCTG CCTCTAGACC AACATCCCGA 
CCCACTCGGG GCAGGGCAAA TAGGTCCTCT ACCAGGACCC CAGAACTGAT TGTCCCTGTA 
GACCCTGAAC TCCAGCCTTC CACCTCCACA GAACAGCCTG TCATCCCAAA ACTTACGTCC 
CAGGTCACTG AGGGCAGGGT ACAGATGCCT GAACCACTTC TCACAGGTCC CGAGATCCAG 
TCTCCCACCT CCACAGAACA GTCTGTCACC CCAGACCGCA AACCTCGGGC CACTCGGGGC 
AGGCCAAGTA AATCTCCCAA CAAGACCCCA GAACCACTTA TTTCTACTGG CCCTGAACTC 
CAACCTCCCA CCTCCATAGA ACAACCTGTC ATACCTAAAC CCACTTCTCG GGTCACTCGG 
GGCAGACCAC GTAAGTCTTC TGTCAGGACC CCAGAATCAG TTGTCTCCAC TGGCCCTGAA 
CTTCAGCCTC TCACCTCTAT AGAACAGCCT GTTATCCCTG AACCTAGGGC CACTCGAGGT 
AGACCAAGTA AGTCTTCTAT CAAGACCCCA GAATCAGTTG TCCCCACTGG TCCTGAGCTG 
CAGCCTCTTA CCTCTGCAAA ACAGCCTGTC ACCCCTAACC TCACATCTCG GGCCTCTCGG 
GGTAGATCAA GTAAGTCTAT CAGGACCCCG GAACCAGTTG TCCAAACTGG CCCTGAATTC 
CATCCATCCA CCTCCACAGA ACAGCCTGAT ACCCGTGAGC CCTCATCTCA GGCTAGGACA 
CGTAGGTCTG CTGTCAAGAC TCCTGAAGCG AGTGTCCCCA CAACCCCTGA ACTCCAGCCT 
TTCACTTCTA AAAAACAGCC TGCCCCTAAG CCTACAGCTC TGGTCACTCA GGGAAGGACA 
TATAAACCCT CCACTGAAGA TTGTGAATCA GTTGGGCCAG TGGCCCCTGA TTTTGAGCCT 
TCCACCTCTA CAGACCATCT TGTCACCCCT AAGGTTACAG ATCAGAGCTT AACACTACAG 
TCTTCACCCC TAAGTGCTTC CCCAGTTTCC AGTACCCCTG ATCTCAAGCC TCCTGTCCCC 
ATAGCCCAGC CTGTTACCCC AGAGCCCATT CCACAAGCCA ATCACCAAAG GAAACGAAGG 
GCTGCTGGGA AGCAGGGTTC CCGCACAGTC CCTCTGGGTC ATAAGTCTTA CTCTGCCCTC 
TCTGAACCTG AGCCCCAATC TTCAGCCAGC CAAAGCTCAG GAGCATCAGA AGCAGACTCA 
CCCCGTCAAA AACGTCCCAG AAGACAAGCC TCCCAGAAAA CTGTAGTCAT CAAGGAAGAA 
CCTGTAGAAA CAGAAGTGAA GGAAGAGCCT CAAGAGACTG CAATTCCAAC ACCAGAAAAG 
AGAAAGAGGG ACCATGCAGA AGAAGTGACC CAGGGAAAAC CAACTCGAAG CCGGCGAACT 
AAACCTAACC AAGAAACAGC CCCCAAGGTA CTGTTCACAG GAGTCATGGA TTCTCGTGGC 
GAGCGTGCAG TGCTGGCTCT GGGAGGCAGT CTGGCCAGCT CAGTAAATGA GGCCTCCCAC 
TTAGTTACTG ATCGCATCCG CAGGACAGTC AAGTTCTTGT GTGCCCTGGG GAAGGGAATT 
CCCATCCTGT CCCTGAACTG GCTGTACCAG TCCCGAAAGG CTGGTTGCTT CTTGCCGCCT 
GATGACTACT TAGTGACTGA TCCTGAGCAA GAGAAGAACT TTAGCTTCAG CCTTCGGGAT 
TCCCTGTGCC GGGCTCGGGA ACGAAGACTG CTGGAGGACT ATGAGATTCA TGTGACCCCT 
GGCGTGCAGC CACCCCCCCC TCAGATGGGC GAGATCATCA GTTGCTGTGG AGGCACTTTC 
CTGCCCAGCA TGCCTCACTC CTATAAGCTT CACCGAGTCA TCATAACTTG CACTGAAGAC 
CTACCACGCT GTGCTATTCC ATCCCGCCTG GGGCTACCCC TCCTCTCTCC TGAGTTCCTC 
CTGACTGGAG TGCTGAAGCA GGAAGCCACA CCAGAGGCCT TTGTTCTCTC CAATTTGGAA 
ATGTCATCTA CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524326.1
CDS	167..4801
Translation	MESTQVIDWDAEEEEETELSSGSLGYSVEPIGQLRLFSGTHGPERDFPLYLGKNVVGRSPDCSVALPFPSISKQHAVIEISAWNKAPILQDCGSLNGTQIVKPPRVLPPGVSHRLRDQELILFADFPCQYHRLDVPPPLVPRSLLTIEKTPRIRIESQNSRVLLAADSEEEGDFPSGRCVANGQRNTASPSATVVPESDEEVSSPAPSVPGPSSPFGLGSDTDEEQGQQPGVEESSLADSSGAAGEAEQPEANGTTAGIQAQPTEHKLKDTKVKKEAGRAGVSDGSVLERSPTLGEDSDTEVDEDHKPGFADSETDVEEERIPVTPPVAPVKKNQVLLAVGIGDPEAPGVAHLQDCLAGSGTDVEDKTALDVPLERNHTPMVINSDTDEEEEEEEEEVSAALTLAHLKERGIGLWSRDPGAEEVKSQPQVLVEQSQSASGRDSDTDVEEESSGRKREIIPDSPMDVDEALTVTQPESQPPRRPNDADEYMDMSSPGSHLVVNQASFAVVGKTRAQVEEEVPGPSVILGEKHQVPLEGAQPPEEAWETAVQEGSSSPEAAASVRPSQQPVAEDAGTECATAVSEQESTLEVRSQSGSPAAPVEQVVIHTDTSGDPTLPQREGAQTPTGREREAHVGRTKSAKECCDAEPEDLCLPATQCFVEGESQHPEAVQSLENEPTQLFPCTLPQEPGPSHLSLQTPGADTLDVPWEVLATQPFCLREQSETSELHEAHGSQPSLPREPPGHQHLVHTSPVHTELLRIEGREIQTVEKAMGRLSCQTTPAGKASRGDPEPPDHCLFSSVPEASTQSLLTSQSQKQSTPQPLFSTSSSEIPLPESLHTKPNVRPRRSSRMTPSPHSSAALKPNTTCPTNQPAASRPTSRPTRGRANRSSTRTPELIVPVDPELQPSTSTEQPVIPKLTSQVTEGRVQMPEPLLTGPEIQSPTSTEQSVTPDRKPRATRGRPSKSPNKTPEPLISTGPELQPPTSIEQPVIPKPTSRVTRGRPRKSSVRTPESVVSTGPELQPLTSIEQPVIPEPRATRGRPSKSSIKTPESVVPTGPELQPLTSAKQPVTPNLTSRASRGRSSKSIRTPEPVVQTGPEFHPSTSTEQPDTREPSSQARTRRSAVKTPEASVPTTPELQPFTSKKQPAPKPTALVTQGRTYKPSTEDCESVGPVAPDFEPSTSTDHLVTPKVTDQSLTLQSSPLSASPVSSTPDLKPPVPIAQPVTPEPIPQANHQRKRRAAGKQGSRTVPLGHKSYSALSEPEPQSSASQSSGASEADSPRQKRPRRQASQKTVVIKEEPVETEVKEEPQETAIPTPEKRKRDHAEEVTQGKPTRSRRTKPNQETAPKVLFTGVMDSRGERAVLALGGSLASSVNEASHLVTDRIRRTVKFLCALGKGIPILSLNWLYQSRKAGCFLPPDDYLVTDPEQEKNFSFSLRDSLCRARERRLLEDYEIHVTPGVQPPPPQMGEIISCCGGTFLPSMPHSYKLHRVIITCTEDLPRCAIPSRLGLPLLSPEFLLTGVLKQEATPEAFVLSNLEMSST

Target ORF information:

RefSeq Version	XM_006524263.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus mediator of DNA damage checkpoint 1 (Mdc1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524263.3

ORF Insert Sequence:

ATGGAAAGCA CCCAGGTGAT TGACTGGGAT GCTGAAGAAG AGGAAGAGAC AGAACTATCC 
AGTGGCTCCT TGGGGTATAG TGTGGAGCCT ATAGGGCAGC TACGTCTCTT CAGTGGAACT 
CATGGACCAG AAAGAGATTT CCCCCTCTAC CTTGGCAAGA ATGTAGTTGG TCGAAGCCCT 
GACTGCTCTG TGGCCCTGCC TTTTCCATCC ATCTCCAAAC AGCATGCAGT AATTGAAATC 
TCAGCTTGGA ACAAAGCCCC TATCCTCCAG GATTGTGGGA GCCTCAATGG CACTCAAATC 
GTAAAGCCTC CTAGGGTCCT ACCTCCTGGA GTGAGCCATC GTCTGAGGGA CCAGGAATTA 
ATTCTGTTTG CAGATTTTCC CTGCCAGTAC CATCGCCTGG ATGTCCCACC ACCCTTGGTC 
CCTCGGAGTC TTCTAACTAT AGAGAAAACC CCCAGAATAC GGATAGAATC CCAAAATTCC 
AGAGTTTTGT TGGCTGCCGA TTCAGAGGAA GAAGGGGATT TTCCTTCTGG AAGGTGTGTG 
GCGAATGGAC AAAGGAATAC AGCATCCCCT TCAGCAACAG TAGTTCCAGA AAGTGATGAA 
GAGGTCTCTT CTCCGGCCCC AAGTGTCCCC GGGCCATCTT CACCCTTTGG TTTGGGCAGT 
GACACTGATG AAGAACAAGG TCAGCAACCA GGAGTAGAGG AGTCCTCTTT AGCTGACAGC 
AGTGGTGCTG CAGGGGAAGC TGAGCAGCCT GAAGCTAACG GAACGACAGC TGGCATACAG 
GCTCAGCCTA CTGAGCACAA ATTGAAGGAC ACAAAAGTCA AGAAGGAGGC AGGCAGGGCA 
GGGGTCTCAG ATGGGTCAGT TCTGGAGAGG AGCCCCACTC TTGGAGAGGA CAGTGACACA 
GAAGTGGACG AAGATCACAA ACCTGGGTTT GCGGACAGTG AGACAGATGT GGAAGAAGAG 
AGGATCCCTG TGACCCCCCC TGTAGCCCCT GTGAAGAAAA ACCAAGTGCT ACTTGCAGTT 
GGTATAGGAG ATCCTGAAGC ACCTGGTGTG GCACATCTGC AGGACTGCCT AGCTGGTAGT 
GGCACAGATG TGGAGGACAA GACTGCACTG GATGTCCCTC TAGAGAGAAA CCACACACCC 
ATGGTGATCA ACAGTGATAC AGATGAGGAG GAGGAGGAGG AGGAGGAGGA GGTCTCAGCA 
GCACTCACTT TGGCCCATCT GAAAGAGAGA GGAATTGGTT TGTGGAGTAG AGACCCAGGC 
GCAGAAGAGG TCAAGTCCCA ACCACAGGTC CTTGTAGAAC AAAGCCAGAG TGCCTCTGGG 
AGAGACAGTG ACACGGATGT GGAGGAGGAA TCCTCAGGGC GAAAGAGAGA GATTATCCCA 
GACAGTCCCA TGGATGTAGA TGAGGCTCTT ACTGTCACAC AGCCAGAGAG CCAGCCTCCC 
CGTAGGCCCA ATGATGCAGA TGAGTACATG GATATGAGCT CCCCTGGCAG CCATCTAGTG 
GTAAATCAGG CCTCCTTTGC TGTGGTAGGC AAGACCAGAG CACAAGTGGA GGAGGAAGTC 
CCAGGGCCAT CTGTTATCCT GGGGGAGAAG CACCAGGTGC CTCTAGAGGG AGCCCAGCCT 
CCTGAGGAAG CCTGGGAAAC AGCTGTTCAG GAAGGCTCAT CCTCACCAGA GGCAGCAGCC 
TCTGTAAGGC CTAGCCAGCA GCCAGTAGCA GAAGATGCTG GGACAGAGTG TGCTACAGCT 
GTTTCTGAGC AGGAAAGTAC TCTTGAGGTG AGGTCCCAAA GCGGGTCACC TGCAGCACCA 
GTGGAGCAAG TGGTGATACA TACAGATACT TCAGGGGATC CCACCCTGCC ACAGAGAGAG 
GGAGCCCAAA CCCCCACAGG AAGGGAGAGA GAAGCACATG TGGGCAGGAC CAAGAGTGCC 
AAAGAGTGCT GTGATGCAGA ACCTGAAGAT CTGTGCCTTC CAGCTACCCA GTGCTTTGTG 
GAAGGGGAGA GCCAGCACCC AGAAGCTGTC CAGAGTTTGG AAAATGAGCC TACCCAACTC 
TTCCCATGTA CTCTTCCACA AGAGCCAGGA CCTTCCCATC TCAGCCTTCA GACTCCAGGT 
GCAGATACCT TGGATGTGCC TTGGGAAGTC TTGGCTACAC AGCCGTTCTG TCTAAGAGAA 
CAGTCAGAGA CTTCTGAGCT TCATGAAGCT CATGGATCTC AACCATCTCT GCCTAGGGAA 
CCACCAGGAC ACCAACATCT GGTTCATACC AGCCCAGTTC ACACAGAGCT GTTGAGAATT 
GAAGGCAGAG AGATACAGAC TGTGGAGAAA GCCATGGGCC GCTTGAGTTG CCAGACGACA 
CCTGCTGGAA AGGCTTCCAG GGGTGATCCA GAGCCCCCAG ACCACTGCCT GTTTTCTTCA 
GTGCCTGAAG CTTCAACTCA GAGTCTCCTC ACCTCTCAGA GCCAAAAGCA GTCTACACCT 
CAGCCTCTGT TTTCGACCTC TTCTTCTGAG ATACCCCTTC CTGAGAGTCT TCACACAAAG 
CCTAATGTCA GGCCACGGCG GTCCTCCAGG ATGACCCCCT CCCCGCATTC CTCTGCTGCC 
CTTAAGCCAA ATACTACCTG CCCCACAAAC CAGCCTGCTG CCTCTAGACC AACATCCCGA 
CCCACTCGGG GCAGGGCAAA TAGGTCCTCT ACCAGGACCC CAGAACTGAT TGTCCCTGTA 
GACCCTGAAC TCCAGCCTTC CACCTCCACA GAACAGCCTG TCATCCCAAA ACTTACGTCC 
CAGGTCACTG AGGGCAGGGT ACAGATGCCT GAACCACTTC TCACAGGTCC CGAGATCCAG 
TCTCCCACCT CCACAGAACA GTCTGTCACC CCAGACCGCA AACCTCGGGC CACTCGGGGC 
AGGCCAAGTA AATCTCCCAA CAAGACCCCA GAACCACTTA TTTCTACTGG CCCTGAACTC 
CAACCTCCCA CCTCCATAGA ACAACCTGTC ATACCTAAAC CCACTTCTCG GGTCACTCGG 
GGCAGACCAC GTAAGTCTTC TGTCAGGACC CCAGAATCAG TTGTCTCCAC TGGCCCTGAA 
CTTCAGCCTC TCACCTCTAT AGAACAGCCT GTTATCCCTG AACCTAGGGC CACTCGAGGT 
AGACCAAGTA AGTCTTCTAT CAAGACCCCA GAATCAGTTG TCCCCACTGG TCCTGAGCTG 
CAGCCTCTTA CCTCTGCAAA ACAGCCTGTC ACCCCTAACC TCACATCTCG GGCCTCTCGG 
GGTAGATCAA GTAAGTCTAT CAGGACCCCG GAACCAGTTG TCCAAACTGG CCCTGAATTC 
CATCCATCCA CCTCCACAGA ACAGCCTGAT ACCCGTGAGC CCTCATCTCA GGCTAGGACA 
CGTAGGTCTG CTGTCAAGAC TCCTGAAGCG AGTGTCCCCA CAACCCCTGA ACTCCAGCCT 
TTCACTTCTA AAAAACAGCC TGCCCCTAAG CCTACAGCTC TGGTCACTCA GGGAAGGACA 
TATAAACCCT CCACTGAAGA TTGTGAATCA GTTGGGCCAG TGGCCCCTGA TTTTGAGCCT 
TCCACCTCTA CAGACCATCT TGTCACCCCT AAGGTTACAG ATCAGAGCTT AACACTACAG 
TCTTCACCCC TAAGTGCTTC CCCAGTTTCC AGTACCCCTG ATCTCAAGCC TCCTGTCCCC 
ATAGCCCAGC CTGTTACCCC AGAGCCCATT CCACAAGCCA ATCACCAAAG GAAACGAAGG 
GCTGCTGGGA AGCAGGGTTC CCGCACAGTC CCTCTGGGTC ATAAGTCTTA CTCTGCCCTC 
TCTGAACCTG AGCCCCAATC TTCAGCCAGC CAAAGCTCAG GAGCATCAGA AGCAGACTCA 
CCCCGTCAAA AACGTCCCAG AAGACAAGCC TCCCAGAAAA CTGTAGTCAT CAAGGAAGAA 
CCTGTAGAAA CAGAAGTGAA GGAAGAGCCT CAAGAGACTG CAATTCCAAC ACCAGAAAAG 
AGAAAGAGGG ACCATGCAGA AGAAGTGACC CAGGGAAAAC CAACTCGAAG CCGGCGAACT 
AAACCTAACC AAGAAACAGC CCCCAAGGTA CTGTTCACAG GAGTCATGGA TTCTCGTGGC 
GAGCGTGCAG TGCTGGCTCT GGGAGGCAGT CTGGCCAGCT CAGTAAATGA GGCCTCCCAC 
TTAGTTACTG ATCGCATCCG CAGGACAGTC AAGTTCTTGT GTGCCCTGGG GAAGGGAATT 
CCCATCCTGT CCCTGAACTG GCTGTACCAG TCCCGAAAGG CTGGTTGCTT CTTGCCGCCT 
GATGACTACT TAGTGACTGA TCCTGAGCAA GAGAAGAACT TTAGCTTCAG CCTTCGGGAT 
TCCCTGTGCC GGGCTCGGGA ACGAAGACTG CTGGAGGACT ATGAGATTCA TGTGACCCCT 
GGCGTGCAGC CACCCCCCCC TCAGATGGGC GAGATCATCA GTTGCTGTGG AGGCACTTTC 
CTGCCCAGCA TGCCTCACTC CTATAAGCTT CACCGAGTCA TCATAACTTG CACTGAAGAC 
CTACCACGCT GTGCTATTCC ATCCCGCCTG GGGCTACCCC TCCTCTCTCC TGAGTTCCTC 
CTGACTGGAG TGCTGAAGCA GGAAGCCACA CCAGAGGCCT TTGTTCTCTC CAATTTGGAA 
ATGTCATCTA CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mdc1 ( XM_006524264.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524327.1 Mus musculus mediator of DNA damage checkpoint 1 (Mdc1), transcript variant X5, mRNA.

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CloneID	OMu53580
Clone ID Related Accession (Same CDS sequence)	XM_006524264.4 , XM_006524264.3
Accession Version	XM_006524264.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4632bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	mediator of DNA damage checkpoint protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006524264.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGCA CCCAGGTGAT TGACTGGGAT GCTGAAGAAG AGGAAGAGAC AGAACTATCC 
AGTGGCTCCT TGGGGTATAG TGTGGAGCCT ATAGGGCAGC TACGTCTCTT CAGTGGAACT 
CATGGACCAG AAAGAGATTT CCCCCTCTAC CTTGGCAAGA ATGTAGTTGG TCGAAGCCCT 
GACTGCTCTG TGGCCCTGCC TTTTCCATCC ATCTCCAAAC AGCATGCAGT AATTGAAATC 
TCAGCTTGGA ACAAAGCCCC TATCCTCCAG GATTGTGGGA GCCTCAATGG CACTCAAATC 
GTAAAGCCTC CTAGGGTCCT ACCTCCTGGA GTGAGCCATC GTCTGAGGGA CCAGGAATTA 
ATTCTGTTTG CAGATTTTCC CTGCCAGTAC CATCGCCTGG ATGTCCCACC ACCCTTGGTC 
CCTCGGAGTC TTCTAACTAT AGAGAAAACC CCCAGAATAC GGATAGAATC CCAAAATTCC 
AGAGTTTTGT TGGCTGCCGA TTCAGAGGAA GAAGGGGATT TTCCTTCTGG AAGGTGTGTG 
GCGAATGGAC AAAGGAATAC AGCATCCCCT TCAGCAACAG TAGTTCCAGA AAGTGATGAA 
GAGGTCTCTT CTCCGGCCCC AAGTGTCCCC GGGCCATCTT CACCCTTTGG TTTGGGCAGT 
GACACTGATG AAGAACAAGG TCAGCAACCA GGAGTAGAGG AGTCCTCTTT AGCTGACAGC 
AGTGGTGCTG CAGGGGAAGC TGAGCAGCCT GAAGCTAACG GAACGACAGC TGGCATACAG 
GCTCAGCCTA CTGAGCACAA ATTGAAGGAC ACAAAAGTCA AGAAGGAGGC AGGCAGGGCA 
GGGGTCTCAG ATGGGTCAGT TCTGGAGAGG AGCCCCACTC TTGGAGAGGA CAGTGACACA 
GAAGTGGACG AAGATCACAA ACCTGGGTTT GCGGACAGTG AGACAGATGT GGAAGAAGAG 
AGGATCCCTG TGACCCCCCC TGTAGCCCCT GTGAAGAAAA ACCAAGTGCT ACTTGCAGTT 
GGTATAGGAG ATCCTGAAGC ACCTGGTGTG GCACATCTGC AGGACTGCCT AGCTGGTAGT 
GGCACAGATG TGGAGGACAA GACTGCACTG GATGTCCCTC TAGAGAGAAA CCACACACCC 
ATGGTGATCA ACAGTGATAC AGATGAGGAG GAGGAGGAGG AGGAGGAGGA GGTCTCAGCA 
GCACTCACTT TGGCCCATCT GAAAGAGAGA GGAATTGGTT TGTGGAGTAG AGACCCAGGC 
GCAGAAGAGG TCAAGTCCCA ACCACAGGTC CTTGTAGAAC AAAGCCAGAG TGCCTCTGGG 
AGAGACAGTG ACACGGATGT GGAGGAGGAA TCCTCAGGGC GAAAGAGAGA GATTATCCCA 
GACAGTCCCA TGGATGTAGA TGAGGCTCTT ACTGTCACAC AGCCAGAGAG CCAGCCTCCC 
CGTAGGCCCA ATGATGCAGA TGAGTACATG GATATGAGCT CCCCTGGCAG CCATCTAGTG 
GTAAATCAGG CCTCCTTTGC TGTGGTAGGC AAGACCAGAG CACAAGTGGA GGAGGAAGTC 
CCAGGGCCAT CTGTTATCCT GGGGGAGAAG CACCAGGTGC CTCTAGAGGG AGCCCAGCCT 
CCTGAGGAAG CCTGGGAAAC AGCTGTTCAG GAAGGCTCAT CCTCACCAGA GGCAGCAGCC 
TCTGTAAGGC CTAGCCAGCA GCCAGTAGCA GAAGATGCTG GGACAGAGTG TGCTACAGCT 
GTTTCTGAGC AGGAAAGTAC TCTTGAGGTG AGGTCCCAAA GCGGGTCACC TGCAGCACCA 
GTGGAGCAAG TGGTGATACA TACAGATACT TCAGGGGATC CCACCCTGCC ACAGAGAGAG 
GGAGCCCAAA CCCCCACAGG AAGGGAGAGA GAAGCACATG TGGGCAGGAC CAAGAGTGCC 
AAAGAGTGCT GTGATGAACC TGAAGATCTG TGCCTTCCAG CTACCCAGTG CTTTGTGGAA 
GGGGAGAGCC AGCACCCAGA AGCTGTCCAG AGTTTGGAAA ATGAGCCTAC CCAACTCTTC 
CCATGTACTC TTCCACAAGA GCCAGGACCT TCCCATCTCA GCCTTCAGAC TCCAGGTGCA 
GATACCTTGG ATGTGCCTTG GGAAGTCTTG GCTACACAGC CGTTCTGTCT AAGAGAACAG 
TCAGAGACTT CTGAGCTTCA TGAAGCTCAT GGATCTCAAC CATCTCTGCC TAGGGAACCA 
CCAGGACACC AACATCTGGT TCATACCAGC CCAGTTCACA CAGAGCTGTT GAGAATTGAA 
GGCAGAGAGA TACAGACTGT GGAGAAAGCC ATGGGCCGCT TGAGTTGCCA GACGACACCT 
GCTGGAAAGG CTTCCAGGGG TGATCCAGAG CCCCCAGACC ACTGCCTGTT TTCTTCAGTG 
CCTGAAGCTT CAACTCAGAG TCTCCTCACC TCTCAGAGCC AAAAGCAGTC TACACCTCAG 
CCTCTGTTTT CGACCTCTTC TTCTGAGATA CCCCTTCCTG AGAGTCTTCA CACAAAGCCT 
AATGTCAGGC CACGGCGGTC CTCCAGGATG ACCCCCTCCC CGCATTCCTC TGCTGCCCTT 
AAGCCAAATA CTACCTGCCC CACAAACCAG CCTGCTGCCT CTAGACCAAC ATCCCGACCC 
ACTCGGGGCA GGGCAAATAG GTCCTCTACC AGGACCCCAG AACTGATTGT CCCTGTAGAC 
CCTGAACTCC AGCCTTCCAC CTCCACAGAA CAGCCTGTCA TCCCAAAACT TACGTCCCAG 
GTCACTGAGG GCAGGGTACA GATGCCTGAA CCACTTCTCA CAGGTCCCGA GATCCAGTCT 
CCCACCTCCA CAGAACAGTC TGTCACCCCA GACCGCAAAC CTCGGGCCAC TCGGGGCAGG 
CCAAGTAAAT CTCCCAACAA GACCCCAGAA CCACTTATTT CTACTGGCCC TGAACTCCAA 
CCTCCCACCT CCATAGAACA ACCTGTCATA CCTAAACCCA CTTCTCGGGT CACTCGGGGC 
AGACCACGTA AGTCTTCTGT CAGGACCCCA GAATCAGTTG TCTCCACTGG CCCTGAACTT 
CAGCCTCTCA CCTCTATAGA ACAGCCTGTT ATCCCTGAAC CTAGGGCCAC TCGAGGTAGA 
CCAAGTAAGT CTTCTATCAA GACCCCAGAA TCAGTTGTCC CCACTGGTCC TGAGCTGCAG 
CCTCTTACCT CTGCAAAACA GCCTGTCACC CCTAACCTCA CATCTCGGGC CTCTCGGGGT 
AGATCAAGTA AGTCTATCAG GACCCCGGAA CCAGTTGTCC AAACTGGCCC TGAATTCCAT 
CCATCCACCT CCACAGAACA GCCTGATACC CGTGAGCCCT CATCTCAGGC TAGGACACGT 
AGGTCTGCTG TCAAGACTCC TGAAGCGAGT GTCCCCACAA CCCCTGAACT CCAGCCTTTC 
ACTTCTAAAA AACAGCCTGC CCCTAAGCCT ACAGCTCTGG TCACTCAGGG AAGGACATAT 
AAACCCTCCA CTGAAGATTG TGAATCAGTT GGGCCAGTGG CCCCTGATTT TGAGCCTTCC 
ACCTCTACAG ACCATCTTGT CACCCCTAAG GTTACAGATC AGAGCTTAAC ACTACAGTCT 
TCACCCCTAA GTGCTTCCCC AGTTTCCAGT ACCCCTGATC TCAAGCCTCC TGTCCCCATA 
GCCCAGCCTG TTACCCCAGA GCCCATTCCA CAAGCCAATC ACCAAAGGAA ACGAAGGGCT 
GCTGGGAAGC AGGGTTCCCG CACAGTCCCT CTGGGTCATA AGTCTTACTC TGCCCTCTCT 
GAACCTGAGC CCCAATCTTC AGCCAGCCAA AGCTCAGGAG CATCAGAAGC AGACTCACCC 
CGTCAAAAAC GTCCCAGAAG ACAAGCCTCC CAGAAAACTG TAGTCATCAA GGAAGAACCT 
GTAGAAACAG AAGTGAAGGA AGAGCCTCAA GAGACTGCAA TTCCAACACC AGAAAAGAGA 
AAGAGGGACC ATGCAGAAGA AGTGACCCAG GGAAAACCAA CTCGAAGCCG GCGAACTAAA 
CCTAACCAAG AAACAGCCCC CAAGGTACTG TTCACAGGAG TCATGGATTC TCGTGGCGAG 
CGTGCAGTGC TGGCTCTGGG AGGCAGTCTG GCCAGCTCAG TAAATGAGGC CTCCCACTTA 
GTTACTGATC GCATCCGCAG GACAGTCAAG TTCTTGTGTG CCCTGGGGAA GGGAATTCCC 
ATCCTGTCCC TGAACTGGCT GTACCAGTCC CGAAAGGCTG GTTGCTTCTT GCCGCCTGAT 
GACTACTTAG TGACTGATCC TGAGCAAGAG AAGAACTTTA GCTTCAGCCT TCGGGATTCC 
CTGTGCCGGG CTCGGGAACG AAGACTGCTG GAGGACTATG AGATTCATGT GACCCCTGGC 
GTGCAGCCAC CCCCCCCTCA GATGGGCGAG ATCATCAGTT GCTGTGGAGG CACTTTCCTG 
CCCAGCATGC CTCACTCCTA TAAGCTTCAC CGAGTCATCA TAACTTGCAC TGAAGACCTA 
CCACGCTGTG CTATTCCATC CCGCCTGGGG CTACCCCTCC TCTCTCCTGA GTTCCTCCTG 
ACTGGAGTGC TGAAGCAGGA AGCCACACCA GAGGCCTTTG TTCTCTCCAA TTTGGAAATG 
TCATCTACCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524327.1
CDS	167..4798
Translation	MESTQVIDWDAEEEEETELSSGSLGYSVEPIGQLRLFSGTHGPERDFPLYLGKNVVGRSPDCSVALPFPSISKQHAVIEISAWNKAPILQDCGSLNGTQIVKPPRVLPPGVSHRLRDQELILFADFPCQYHRLDVPPPLVPRSLLTIEKTPRIRIESQNSRVLLAADSEEEGDFPSGRCVANGQRNTASPSATVVPESDEEVSSPAPSVPGPSSPFGLGSDTDEEQGQQPGVEESSLADSSGAAGEAEQPEANGTTAGIQAQPTEHKLKDTKVKKEAGRAGVSDGSVLERSPTLGEDSDTEVDEDHKPGFADSETDVEEERIPVTPPVAPVKKNQVLLAVGIGDPEAPGVAHLQDCLAGSGTDVEDKTALDVPLERNHTPMVINSDTDEEEEEEEEEVSAALTLAHLKERGIGLWSRDPGAEEVKSQPQVLVEQSQSASGRDSDTDVEEESSGRKREIIPDSPMDVDEALTVTQPESQPPRRPNDADEYMDMSSPGSHLVVNQASFAVVGKTRAQVEEEVPGPSVILGEKHQVPLEGAQPPEEAWETAVQEGSSSPEAAASVRPSQQPVAEDAGTECATAVSEQESTLEVRSQSGSPAAPVEQVVIHTDTSGDPTLPQREGAQTPTGREREAHVGRTKSAKECCDEPEDLCLPATQCFVEGESQHPEAVQSLENEPTQLFPCTLPQEPGPSHLSLQTPGADTLDVPWEVLATQPFCLREQSETSELHEAHGSQPSLPREPPGHQHLVHTSPVHTELLRIEGREIQTVEKAMGRLSCQTTPAGKASRGDPEPPDHCLFSSVPEASTQSLLTSQSQKQSTPQPLFSTSSSEIPLPESLHTKPNVRPRRSSRMTPSPHSSAALKPNTTCPTNQPAASRPTSRPTRGRANRSSTRTPELIVPVDPELQPSTSTEQPVIPKLTSQVTEGRVQMPEPLLTGPEIQSPTSTEQSVTPDRKPRATRGRPSKSPNKTPEPLISTGPELQPPTSIEQPVIPKPTSRVTRGRPRKSSVRTPESVVSTGPELQPLTSIEQPVIPEPRATRGRPSKSSIKTPESVVPTGPELQPLTSAKQPVTPNLTSRASRGRSSKSIRTPEPVVQTGPEFHPSTSTEQPDTREPSSQARTRRSAVKTPEASVPTTPELQPFTSKKQPAPKPTALVTQGRTYKPSTEDCESVGPVAPDFEPSTSTDHLVTPKVTDQSLTLQSSPLSASPVSSTPDLKPPVPIAQPVTPEPIPQANHQRKRRAAGKQGSRTVPLGHKSYSALSEPEPQSSASQSSGASEADSPRQKRPRRQASQKTVVIKEEPVETEVKEEPQETAIPTPEKRKRDHAEEVTQGKPTRSRRTKPNQETAPKVLFTGVMDSRGERAVLALGGSLASSVNEASHLVTDRIRRTVKFLCALGKGIPILSLNWLYQSRKAGCFLPPDDYLVTDPEQEKNFSFSLRDSLCRARERRLLEDYEIHVTPGVQPPPPQMGEIISCCGGTFLPSMPHSYKLHRVIITCTEDLPRCAIPSRLGLPLLSPEFLLTGVLKQEATPEAFVLSNLEMSST

Target ORF information:

RefSeq Version	XM_006524264.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus mediator of DNA damage checkpoint 1 (Mdc1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524264.3

ORF Insert Sequence:

ATGGAAAGCA CCCAGGTGAT TGACTGGGAT GCTGAAGAAG AGGAAGAGAC AGAACTATCC 
AGTGGCTCCT TGGGGTATAG TGTGGAGCCT ATAGGGCAGC TACGTCTCTT CAGTGGAACT 
CATGGACCAG AAAGAGATTT CCCCCTCTAC CTTGGCAAGA ATGTAGTTGG TCGAAGCCCT 
GACTGCTCTG TGGCCCTGCC TTTTCCATCC ATCTCCAAAC AGCATGCAGT AATTGAAATC 
TCAGCTTGGA ACAAAGCCCC TATCCTCCAG GATTGTGGGA GCCTCAATGG CACTCAAATC 
GTAAAGCCTC CTAGGGTCCT ACCTCCTGGA GTGAGCCATC GTCTGAGGGA CCAGGAATTA 
ATTCTGTTTG CAGATTTTCC CTGCCAGTAC CATCGCCTGG ATGTCCCACC ACCCTTGGTC 
CCTCGGAGTC TTCTAACTAT AGAGAAAACC CCCAGAATAC GGATAGAATC CCAAAATTCC 
AGAGTTTTGT TGGCTGCCGA TTCAGAGGAA GAAGGGGATT TTCCTTCTGG AAGGTGTGTG 
GCGAATGGAC AAAGGAATAC AGCATCCCCT TCAGCAACAG TAGTTCCAGA AAGTGATGAA 
GAGGTCTCTT CTCCGGCCCC AAGTGTCCCC GGGCCATCTT CACCCTTTGG TTTGGGCAGT 
GACACTGATG AAGAACAAGG TCAGCAACCA GGAGTAGAGG AGTCCTCTTT AGCTGACAGC 
AGTGGTGCTG CAGGGGAAGC TGAGCAGCCT GAAGCTAACG GAACGACAGC TGGCATACAG 
GCTCAGCCTA CTGAGCACAA ATTGAAGGAC ACAAAAGTCA AGAAGGAGGC AGGCAGGGCA 
GGGGTCTCAG ATGGGTCAGT TCTGGAGAGG AGCCCCACTC TTGGAGAGGA CAGTGACACA 
GAAGTGGACG AAGATCACAA ACCTGGGTTT GCGGACAGTG AGACAGATGT GGAAGAAGAG 
AGGATCCCTG TGACCCCCCC TGTAGCCCCT GTGAAGAAAA ACCAAGTGCT ACTTGCAGTT 
GGTATAGGAG ATCCTGAAGC ACCTGGTGTG GCACATCTGC AGGACTGCCT AGCTGGTAGT 
GGCACAGATG TGGAGGACAA GACTGCACTG GATGTCCCTC TAGAGAGAAA CCACACACCC 
ATGGTGATCA ACAGTGATAC AGATGAGGAG GAGGAGGAGG AGGAGGAGGA GGTCTCAGCA 
GCACTCACTT TGGCCCATCT GAAAGAGAGA GGAATTGGTT TGTGGAGTAG AGACCCAGGC 
GCAGAAGAGG TCAAGTCCCA ACCACAGGTC CTTGTAGAAC AAAGCCAGAG TGCCTCTGGG 
AGAGACAGTG ACACGGATGT GGAGGAGGAA TCCTCAGGGC GAAAGAGAGA GATTATCCCA 
GACAGTCCCA TGGATGTAGA TGAGGCTCTT ACTGTCACAC AGCCAGAGAG CCAGCCTCCC 
CGTAGGCCCA ATGATGCAGA TGAGTACATG GATATGAGCT CCCCTGGCAG CCATCTAGTG 
GTAAATCAGG CCTCCTTTGC TGTGGTAGGC AAGACCAGAG CACAAGTGGA GGAGGAAGTC 
CCAGGGCCAT CTGTTATCCT GGGGGAGAAG CACCAGGTGC CTCTAGAGGG AGCCCAGCCT 
CCTGAGGAAG CCTGGGAAAC AGCTGTTCAG GAAGGCTCAT CCTCACCAGA GGCAGCAGCC 
TCTGTAAGGC CTAGCCAGCA GCCAGTAGCA GAAGATGCTG GGACAGAGTG TGCTACAGCT 
GTTTCTGAGC AGGAAAGTAC TCTTGAGGTG AGGTCCCAAA GCGGGTCACC TGCAGCACCA 
GTGGAGCAAG TGGTGATACA TACAGATACT TCAGGGGATC CCACCCTGCC ACAGAGAGAG 
GGAGCCCAAA CCCCCACAGG AAGGGAGAGA GAAGCACATG TGGGCAGGAC CAAGAGTGCC 
AAAGAGTGCT GTGATGAACC TGAAGATCTG TGCCTTCCAG CTACCCAGTG CTTTGTGGAA 
GGGGAGAGCC AGCACCCAGA AGCTGTCCAG AGTTTGGAAA ATGAGCCTAC CCAACTCTTC 
CCATGTACTC TTCCACAAGA GCCAGGACCT TCCCATCTCA GCCTTCAGAC TCCAGGTGCA 
GATACCTTGG ATGTGCCTTG GGAAGTCTTG GCTACACAGC CGTTCTGTCT AAGAGAACAG 
TCAGAGACTT CTGAGCTTCA TGAAGCTCAT GGATCTCAAC CATCTCTGCC TAGGGAACCA 
CCAGGACACC AACATCTGGT TCATACCAGC CCAGTTCACA CAGAGCTGTT GAGAATTGAA 
GGCAGAGAGA TACAGACTGT GGAGAAAGCC ATGGGCCGCT TGAGTTGCCA GACGACACCT 
GCTGGAAAGG CTTCCAGGGG TGATCCAGAG CCCCCAGACC ACTGCCTGTT TTCTTCAGTG 
CCTGAAGCTT CAACTCAGAG TCTCCTCACC TCTCAGAGCC AAAAGCAGTC TACACCTCAG 
CCTCTGTTTT CGACCTCTTC TTCTGAGATA CCCCTTCCTG AGAGTCTTCA CACAAAGCCT 
AATGTCAGGC CACGGCGGTC CTCCAGGATG ACCCCCTCCC CGCATTCCTC TGCTGCCCTT 
AAGCCAAATA CTACCTGCCC CACAAACCAG CCTGCTGCCT CTAGACCAAC ATCCCGACCC 
ACTCGGGGCA GGGCAAATAG GTCCTCTACC AGGACCCCAG AACTGATTGT CCCTGTAGAC 
CCTGAACTCC AGCCTTCCAC CTCCACAGAA CAGCCTGTCA TCCCAAAACT TACGTCCCAG 
GTCACTGAGG GCAGGGTACA GATGCCTGAA CCACTTCTCA CAGGTCCCGA GATCCAGTCT 
CCCACCTCCA CAGAACAGTC TGTCACCCCA GACCGCAAAC CTCGGGCCAC TCGGGGCAGG 
CCAAGTAAAT CTCCCAACAA GACCCCAGAA CCACTTATTT CTACTGGCCC TGAACTCCAA 
CCTCCCACCT CCATAGAACA ACCTGTCATA CCTAAACCCA CTTCTCGGGT CACTCGGGGC 
AGACCACGTA AGTCTTCTGT CAGGACCCCA GAATCAGTTG TCTCCACTGG CCCTGAACTT 
CAGCCTCTCA CCTCTATAGA ACAGCCTGTT ATCCCTGAAC CTAGGGCCAC TCGAGGTAGA 
CCAAGTAAGT CTTCTATCAA GACCCCAGAA TCAGTTGTCC CCACTGGTCC TGAGCTGCAG 
CCTCTTACCT CTGCAAAACA GCCTGTCACC CCTAACCTCA CATCTCGGGC CTCTCGGGGT 
AGATCAAGTA AGTCTATCAG GACCCCGGAA CCAGTTGTCC AAACTGGCCC TGAATTCCAT 
CCATCCACCT CCACAGAACA GCCTGATACC CGTGAGCCCT CATCTCAGGC TAGGACACGT 
AGGTCTGCTG TCAAGACTCC TGAAGCGAGT GTCCCCACAA CCCCTGAACT CCAGCCTTTC 
ACTTCTAAAA AACAGCCTGC CCCTAAGCCT ACAGCTCTGG TCACTCAGGG AAGGACATAT 
AAACCCTCCA CTGAAGATTG TGAATCAGTT GGGCCAGTGG CCCCTGATTT TGAGCCTTCC 
ACCTCTACAG ACCATCTTGT CACCCCTAAG GTTACAGATC AGAGCTTAAC ACTACAGTCT 
TCACCCCTAA GTGCTTCCCC AGTTTCCAGT ACCCCTGATC TCAAGCCTCC TGTCCCCATA 
GCCCAGCCTG TTACCCCAGA GCCCATTCCA CAAGCCAATC ACCAAAGGAA ACGAAGGGCT 
GCTGGGAAGC AGGGTTCCCG CACAGTCCCT CTGGGTCATA AGTCTTACTC TGCCCTCTCT 
GAACCTGAGC CCCAATCTTC AGCCAGCCAA AGCTCAGGAG CATCAGAAGC AGACTCACCC 
CGTCAAAAAC GTCCCAGAAG ACAAGCCTCC CAGAAAACTG TAGTCATCAA GGAAGAACCT 
GTAGAAACAG AAGTGAAGGA AGAGCCTCAA GAGACTGCAA TTCCAACACC AGAAAAGAGA 
AAGAGGGACC ATGCAGAAGA AGTGACCCAG GGAAAACCAA CTCGAAGCCG GCGAACTAAA 
CCTAACCAAG AAACAGCCCC CAAGGTACTG TTCACAGGAG TCATGGATTC TCGTGGCGAG 
CGTGCAGTGC TGGCTCTGGG AGGCAGTCTG GCCAGCTCAG TAAATGAGGC CTCCCACTTA 
GTTACTGATC GCATCCGCAG GACAGTCAAG TTCTTGTGTG CCCTGGGGAA GGGAATTCCC 
ATCCTGTCCC TGAACTGGCT GTACCAGTCC CGAAAGGCTG GTTGCTTCTT GCCGCCTGAT 
GACTACTTAG TGACTGATCC TGAGCAAGAG AAGAACTTTA GCTTCAGCCT TCGGGATTCC 
CTGTGCCGGG CTCGGGAACG AAGACTGCTG GAGGACTATG AGATTCATGT GACCCCTGGC 
GTGCAGCCAC CCCCCCCTCA GATGGGCGAG ATCATCAGTT GCTGTGGAGG CACTTTCCTG 
CCCAGCATGC CTCACTCCTA TAAGCTTCAC CGAGTCATCA TAACTTGCAC TGAAGACCTA 
CCACGCTGTG CTATTCCATC CCGCCTGGGG CTACCCCTCC TCTCTCCTGA GTTCCTCCTG 
ACTGGAGTGC TGAAGCAGGA AGCCACACCA GAGGCCTTTG TTCTCTCCAA TTTGGAAATG 
TCATCTACCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Mdc1
Entrez Gene ID	240087
Full Name	mediator of DNA damage checkpoint 1
Synonyms	6820401C03,AA413496,Nfbd1,mKIAA0170
General protein information	Preferred Names mediator of DNA damage checkpoint 1 Names mediator of DNA damage checkpoint protein 1 mediation of DNA damage checkpoint 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17\|17 B1
Summary	The protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 7 divergent copies of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci. Mice with mutations in this gene exhibit growth retardation, male infertility, immune defects, chromosome instability, DNA repair defects, and radiation sensitivity. [provided by RefSeq, Jul 2008].

	MDC1	NP_001108419.1
Pan troglodytes (chimpanzee)	MDC1	NP_001035841.1
Canis lupus familiaris (dog)	MDC1	XP_005627164.1
Homo sapiens (human)	MDC1	NP_055456.2
Bos taurus (cattle)	MDC1	XP_002697401.2
Mus musculus (house mouse)	Mdc1	NP_001010833.2
Rattus norvegicus (Norway rat)	Mdc1	NP_001159747.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Mdc1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

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