PCBP1 cDNA ORF clone, Mus musculus(House mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	PCBP1
Entrez Gene ID	23983
Full Name	poly(rC) binding protein 1
Synonyms	WBP17,[a]CP-1,alphaCP-1,hnRNP E1,hnRNP-E1
General protein information	Preferred Names poly(rC) binding protein 1 Names poly(rC)-binding protein 1 alpha-CP1 heterogeneous nuclear ribonucleoprotein E1
Gene Type	protein-coding
Organism	Mus musculus(House mouse)
Genome	Chromosome: 6 Map Location: 6\|6 D1

mRNA and Protein(s)

mRNA	Protein	Name
NM_011865.4	NP_035995.1	poly(rC)-binding protein 1
NM_011865.3	NP_035995.1	poly(rC)-binding protein 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	PCBP1	NP_006187.2
Canis lupus familiaris (dog)	PCBP1	XP_538537.1
Bos taurus (cattle)	PCBP1	NP_001015565.1
Rattus norvegicus (Norway rat)	Pcbp1	XP_006236906.1
Pan troglodytes (chimpanzee)	PCBP1	XP_515530.2
Mus musculus (house mouse)	Pcbp1	NP_035995.1

Gene Ontology
Bibliography

Related articles in PubMed

Poly(C)-binding protein 1 (Pcbp1) regulates skeletal muscle differentiation by modulating microRNA processing in myoblasts.
Espinoza-Lewis RA, Yang Q, Liu J, Huang ZP, Hu X, Chen D, Wang DZ
The Journal of biological chemistry292(23)9540-9550(2017 06)

PCBP1 and NCOA4 regulate erythroid iron storage and heme biosynthesis.
Ryu MS, Zhang D, Protchenko O, Shakoury-Elizeh M, Philpott CC
The Journal of clinical investigation127(5)1786-1797(2017 May)

Mutant HSPB1 causes loss of translational repression by binding to PCBP1, an RNA binding protein with a possible role in neurodegenerative disease.
Geuens T, De Winter V, Rajan N, Achsel T, Mateiu L, Almeida-Souza L, Asselbergh B, Bouhy D, Auer-Grumbach M, Bagni C, Timmerman V
Acta neuropathologica communications5(1)5(2017 Jan)

The Poly(C) Binding Protein Pcbp2 and Its Retrotransposed Derivative Pcbp1 Are Independently Essential to Mouse Development.
Ghanem LR, Kromer A, Silverman IM, Chatterji P, Traxler E, Penzo-Mendez A, Weiss MJ, Stanger BZ, Liebhaber SA
Molecular and cellular biology36(2)304-19(2015 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Pcbp1 modulated the processing of muscle-enriched miR-1, miR-133, and miR-206 by physically interacting with argonaute 2 (AGO2) and other miRNA pathway components.
Title: Poly(C)-binding protein 1 (Pcbp1) regulates skeletal muscle differentiation by modulating microRNA processing in myoblasts.

These studies demonstrate the importance of ferritin for the vectorial transfer of imported iron to mitochondria in developing red cells and of PCBP1 and NCOA4 in mediating iron flux through ferritin.
Title: PCBP1 and NCOA4 regulate erythroid iron storage and heme biosynthesis.

Study report a novel interaction between mutant HSPB1-P182L and the RNA binding protein PCBP1, leading to a reduction in its translational repression activity. Identifying PCBP1 mRNA targets revealed a marked prevalence for an RNA recognition motif, preferably seen in their 5' and 3'UTRs. Findings further support a role for mutant HSPB1 in neurodegenerative diseases.
Title: Mutant HSPB1 causes loss of translational repression by binding to PCBP1, an RNA binding protein with a possible role in neurodegenerative disease.

These data reveal that Pcbp1 and Pcbp2 are individually essential for mouse embryonic development and have distinct impacts on embryonic viability and that Pcpb2 has a nonredundant in vivo role in hematopoiesis.
Title: The Poly(C) Binding Protein Pcbp2 and Its Retrotransposed Derivative Pcbp1 Are Independently Essential to Mouse Development.

PCBP1 may serve as a novel maternal factor.
Title: Maternal PCBP1 determines the normal timing of pronucleus formation in mouse eggs.

The following PCBP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PCBP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu58154	NM_011865.4 Latest version!	Mus musculus poly(rC) binding protein 1 (Pcbp1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
View Old Accession Versions >>
OMu58154	NM_011865.3	Mus musculus poly(rC) binding protein 1 (Pcbp1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PCBP1 ( NM_011865.4 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_035995.1 Mus musculus poly(rC) binding protein 1 (Pcbp1), mRNA.

Price & Availability↑

CloneID	OMu58154
Clone ID Related Accession (Same CDS sequence)	NM_011865.4 , NM_011865.3
Accession Version	NM_011865.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1071bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-25
Organism	Mus musculus(house mouse)
Product	poly(rC)-binding protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC153374.5. On Sep 28, 2017 this sequence version replaced NM_011865.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.

ATGGACGCCG GTGTGACTGA AAGCGGACTC AACGTGACTC TCACCATTCG GCTGCTGATG 
CACGGAAAGG AAGTAGGCAG CATCATCGGG AAGAAAGGGG AGTCGGTGAA GAGGATCCGC 
GAGGAGAGCG GCGCGCGGAT CAACATCTCG GAGGGGAACT GCCCGGAGAG GATCATCACG 
CTGACTGGGC CTACCAATGC CATCTTTAAG GCCTTCGCCA TGATCATCGA CAAGCTGGAG 
GAAGATATCA ACAGCTCCAT GACCAACAGT ACGGCGGCCA GCAGGCCCCC GGTCACACTT 
CGGCTGGTCG TGCCCGCCAC CCAGTGTGGC TCCCTGATCG GCAAGGGCGG CTGCAAGATC 
AAGGAGATCC GCGAGAGCAC CGGGGCCCAG GTCCAGGTGG CGGGGGATAT GCTGCCCAAC 
TCGACCGAGC GGGCTATCAC TATCGCCGGC GTGCCGCAGT CCGTCACCGA GTGTGTGAAG 
CAGATCTGCC TGGTCATGCT GGAGACGCTC TCCCAGTCTC CACAGGGGAG AGTCATGACC 
ATCCCGTACC AGCCCATGCC GGCCAGCTCG CCAGTCATCT GCGCGGGCGG CCAAGATCGC 
TGCAGCGACG CGGCGGGCTA CCCCCACGCC ACCCACGACC TGGAGGGACC ACCTCTAGAC 
GCCTACTCGA TTCAAGGACA ACACACCATT TCTCCGCTGG ATCTGGCCAA GCTGAACCAG 
GTGGCAAGAC AACAGTCTCA CTTTGCCATG ATGCACGGCG GGACGGGATT CGCCGGAATT 
GACTCCAGCT CTCCAGAGGT GAAAGGCTAT TGGGCAAGTT TGGATGCATC TACTCAAACC 
ACCCATGAAC TCACCATTCC AAATAACTTA ATCGGCTGCA TAATCGGGCG CCAAGGCGCC 
AACATCAATG AGATCCGCCA GATGTCCGGG GCCCAGATCA AAATTGCCAA CCCGGTGGAA 
GGCTCTTCTG GAAGGCAGGT CACTATTACT GGCTCGGCTG CCAGTATTAG CCTGGCCCAG 
TATCTAATCA ATGCCAGGCT TTCCTCTGAG AAGGGGATGG GGTGCAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035995.1
CDS	275..1345
Translation	MDAGVTESGLNVTLTIRLLMHGKEVGSIIGKKGESVKRIREESGARINISEGNCPERIITLTGPTNAIFKAFAMIIDKLEEDINSSMTNSTAASRPPVTLRLVVPATQCGSLIGKGGCKIKEIRESTGAQVQVAGDMLPNSTERAITIAGVPQSVTECVKQICLVMLETLSQSPQGRVMTIPYQPMPASSPVICAGGQDRCSDAAGYPHATHDLEGPPLDAYSIQGQHTISPLDLAKLNQVARQQSHFAMMHGGTGFAGIDSSSPEVKGYWASLDASTQTTHELTIPNNLIGCIIGRQGANINEIRQMSGAQIKIANPVEGSSGRQVTITGSAASISLAQYLINARLSSEKGMGCS

Target ORF information:

RefSeq Version	NM_011865.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus poly(rC) binding protein 1 (Pcbp1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011865.4

ORF Insert Sequence:

ATGGACGCCG GTGTGACTGA AAGCGGACTC AACGTGACTC TCACCATTCG GCTGCTGATG 
CACGGAAAGG AAGTAGGCAG CATCATCGGG AAGAAAGGGG AGTCGGTGAA GAGGATCCGC 
GAGGAGAGCG GCGCGCGGAT CAACATCTCG GAGGGGAACT GCCCGGAGAG GATCATCACG 
CTGACTGGGC CTACCAATGC CATCTTTAAG GCCTTCGCCA TGATCATCGA CAAGCTGGAG 
GAAGATATCA ACAGCTCCAT GACCAACAGT ACGGCGGCCA GCAGGCCCCC GGTCACACTT 
CGGCTGGTCG TGCCCGCCAC CCAGTGTGGC TCCCTGATCG GCAAGGGCGG CTGCAAGATC 
AAGGAGATCC GCGAGAGCAC CGGGGCCCAG GTCCAGGTGG CGGGGGATAT GCTGCCCAAC 
TCGACCGAGC GGGCTATCAC TATCGCCGGC GTGCCGCAGT CCGTCACCGA GTGTGTGAAG 
CAGATCTGCC TGGTCATGCT GGAGACGCTC TCCCAGTCTC CACAGGGGAG AGTCATGACC 
ATCCCGTACC AGCCCATGCC GGCCAGCTCG CCAGTCATCT GCGCGGGCGG CCAAGATCGC 
TGCAGCGACG CGGCGGGCTA CCCCCACGCC ACCCACGACC TGGAGGGACC ACCTCTAGAC 
GCCTACTCGA TTCAAGGACA ACACACCATT TCTCCGCTGG ATCTGGCCAA GCTGAACCAG 
GTGGCAAGAC AACAGTCTCA CTTTGCCATG ATGCACGGCG GGACGGGATT CGCCGGAATT 
GACTCCAGCT CTCCAGAGGT GAAAGGCTAT TGGGCAAGTT TGGATGCATC TACTCAAACC 
ACCCATGAAC TCACCATTCC AAATAACTTA ATCGGCTGCA TAATCGGGCG CCAAGGCGCC 
AACATCAATG AGATCCGCCA GATGTCCGGG GCCCAGATCA AAATTGCCAA CCCGGTGGAA 
GGCTCTTCTG GAAGGCAGGT CACTATTACT GGCTCGGCTG CCAGTATTAG CCTGGCCCAG 
TATCTAATCA ATGCCAGGCT TTCCTCTGAG AAGGGGATGG GGTGCAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCBP1 ( NM_011865.3 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_035995.1 Mus musculus poly(rC) binding protein 1 (Pcbp1), mRNA.

Price & Availability↑

CloneID	OMu58154
Clone ID Related Accession (Same CDS sequence)	NM_011865.4 , NM_011865.3
Accession Version	NM_011865.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1071bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-12
Organism	Mus musculus(House mouse)
Product	poly(rC)-binding protein 1
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC069915.1. On May 16, 2004 this sequence version replaced NM_011865.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: BC069915.1 [ECO:0000345] ##Evidence-Data-END##

ATGGACGCCG GTGTGACTGA AAGCGGACTC AACGTGACTC TCACCATTCG GCTGCTGATG 
CACGGAAAGG AAGTAGGCAG CATCATCGGG AAGAAAGGGG AGTCGGTGAA GAGGATCCGC 
GAGGAGAGCG GCGCGCGGAT CAACATCTCG GAGGGGAACT GCCCGGAGAG GATCATCACG 
CTGACTGGGC CTACCAATGC CATCTTTAAG GCCTTCGCCA TGATCATCGA CAAGCTGGAG 
GAAGATATCA ACAGCTCCAT GACCAACAGT ACGGCGGCCA GCAGGCCCCC GGTCACACTT 
CGGCTGGTCG TGCCCGCCAC CCAGTGTGGC TCCCTGATCG GCAAGGGCGG CTGCAAGATC 
AAGGAGATCC GCGAGAGCAC CGGGGCCCAG GTCCAGGTGG CGGGGGATAT GCTGCCCAAC 
TCGACCGAGC GGGCTATCAC TATCGCCGGC GTGCCGCAGT CCGTCACCGA GTGTGTGAAG 
CAGATCTGCC TGGTCATGCT GGAGACGCTC TCCCAGTCTC CACAGGGGAG AGTCATGACC 
ATCCCGTACC AGCCCATGCC GGCCAGCTCG CCAGTCATCT GCGCGGGCGG CCAAGATCGC 
TGCAGCGACG CGGCGGGCTA CCCCCACGCC ACCCACGACC TGGAGGGACC ACCTCTAGAC 
GCCTACTCGA TTCAAGGACA ACACACCATT TCTCCGCTGG ATCTGGCCAA GCTGAACCAG 
GTGGCAAGAC AACAGTCTCA CTTTGCCATG ATGCACGGCG GGACGGGATT CGCCGGAATT 
GACTCCAGCT CTCCAGAGGT GAAAGGCTAT TGGGCAAGTT TGGATGCATC TACTCAAACC 
ACCCATGAAC TCACCATTCC AAATAACTTA ATCGGCTGCA TAATCGGGCG CCAAGGCGCC 
AACATCAATG AGATCCGCCA GATGTCCGGG GCCCAGATCA AAATTGCCAA CCCGGTGGAA 
GGCTCTTCTG GAAGGCAGGT CACTATTACT GGCTCGGCTG CCAGTATTAG CCTGGCCCAG 
TATCTAATCA ATGCCAGGCT TTCCTCTGAG AAGGGGATGG GGTGCAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035995.1
CDS	263..1333
Translation	MDAGVTESGLNVTLTIRLLMHGKEVGSIIGKKGESVKRIREESGARINISEGNCPERIITLTGPTNAIFKAFAMIIDKLEEDINSSMTNSTAASRPPVTLRLVVPATQCGSLIGKGGCKIKEIRESTGAQVQVAGDMLPNSTERAITIAGVPQSVTECVKQICLVMLETLSQSPQGRVMTIPYQPMPASSPVICAGGQDRCSDAAGYPHATHDLEGPPLDAYSIQGQHTISPLDLAKLNQVARQQSHFAMMHGGTGFAGIDSSSPEVKGYWASLDASTQTTHELTIPNNLIGCIIGRQGANINEIRQMSGAQIKIANPVEGSSGRQVTITGSAASISLAQYLINARLSSEKGMGCS

Target ORF information:

RefSeq Version	NM_011865.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus poly(rC) binding protein 1 (Pcbp1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011865.3

ORF Insert Sequence:

ATGGACGCCG GTGTGACTGA AAGCGGACTC AACGTGACTC TCACCATTCG GCTGCTGATG 
CACGGAAAGG AAGTAGGCAG CATCATCGGG AAGAAAGGGG AGTCGGTGAA GAGGATCCGC 
GAGGAGAGCG GCGCGCGGAT CAACATCTCG GAGGGGAACT GCCCGGAGAG GATCATCACG 
CTGACTGGGC CTACCAATGC CATCTTTAAG GCCTTCGCCA TGATCATCGA CAAGCTGGAG 
GAAGATATCA ACAGCTCCAT GACCAACAGT ACGGCGGCCA GCAGGCCCCC GGTCACACTT 
CGGCTGGTCG TGCCCGCCAC CCAGTGTGGC TCCCTGATCG GCAAGGGCGG CTGCAAGATC 
AAGGAGATCC GCGAGAGCAC CGGGGCCCAG GTCCAGGTGG CGGGGGATAT GCTGCCCAAC 
TCGACCGAGC GGGCTATCAC TATCGCCGGC GTGCCGCAGT CCGTCACCGA GTGTGTGAAG 
CAGATCTGCC TGGTCATGCT GGAGACGCTC TCCCAGTCTC CACAGGGGAG AGTCATGACC 
ATCCCGTACC AGCCCATGCC GGCCAGCTCG CCAGTCATCT GCGCGGGCGG CCAAGATCGC 
TGCAGCGACG CGGCGGGCTA CCCCCACGCC ACCCACGACC TGGAGGGACC ACCTCTAGAC 
GCCTACTCGA TTCAAGGACA ACACACCATT TCTCCGCTGG ATCTGGCCAA GCTGAACCAG 
GTGGCAAGAC AACAGTCTCA CTTTGCCATG ATGCACGGCG GGACGGGATT CGCCGGAATT 
GACTCCAGCT CTCCAGAGGT GAAAGGCTAT TGGGCAAGTT TGGATGCATC TACTCAAACC 
ACCCATGAAC TCACCATTCC AAATAACTTA ATCGGCTGCA TAATCGGGCG CCAAGGCGCC 
AACATCAATG AGATCCGCCA GATGTCCGGG GCCCAGATCA AAATTGCCAA CCCGGTGGAA 
GGCTCTTCTG GAAGGCAGGT CACTATTACT GGCTCGGCTG CCAGTATTAG CCTGGCCCAG 
TATCTAATCA ATGCCAGGCT TTCCTCTGAG AAGGGGATGG GGTGCAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Poly(C)-binding protein 1 (Pcbp1) regulates skeletal muscle differentiation by modulating microRNA processing in myoblasts.
The Journal of biological chemistry292(23)9540-9550(2017 06)
Espinoza-Lewis RA,Yang Q,Liu J,Huang ZP,Hu X,Chen D,Wang DZ

PCBP1 and NCOA4 regulate erythroid iron storage and heme biosynthesis.
The Journal of clinical investigation127(5)1786-1797(2017 May)
Ryu MS,Zhang D,Protchenko O,Shakoury-Elizeh M,Philpott CC

Mutant HSPB1 causes loss of translational repression by binding to PCBP1, an RNA binding protein with a possible role in neurodegenerative disease.
Acta neuropathologica communications5(1)5(2017 Jan)
Geuens T,De Winter V,Rajan N,Achsel T,Mateiu L,Almeida-Souza L,Asselbergh B,Bouhy D,Auer-Grumbach M,Bagni C,Timmerman V

The Poly(C) Binding Protein Pcbp2 and Its Retrotransposed Derivative Pcbp1 Are Independently Essential to Mouse Development.
Molecular and cellular biology36(2)304-19(2015 Nov)
Ghanem LR,Kromer A,Silverman IM,Chatterji P,Traxler E,Penzo-Mendez A,Weiss MJ,Stanger BZ,Liebhaber SA

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PCBP1 cDNA ORF clone, Mus musculus(House mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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