Cacna1i cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cacna1i
Entrez Gene ID	239556
Full Name	calcium channel, voltage-dependent, alpha 1I subunit
Synonyms	Ca(V)3.3
General protein information	Preferred Names calcium channel, voltage-dependent, alpha 1I subunit Names voltage-dependent T-type calcium channel subunit alpha-1I low voltage-activated T-type calcium channel alpha-1 subunit (CACNA1I) low-voltage-activated calcium channel alpha13.3
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 15 Map Location: 15\|15 E1

mRNA and Protein(s)

mRNA	Protein	Name
XM_030248558.1	XP_030104418.1	voltage-dependent T-type calcium channel subunit alpha-1I isoform X3
XM_006520949.2	XP_006521012.1	voltage-dependent T-type calcium channel subunit alpha-1I isoform X1
XM_006520950.2	XP_006521013.1	voltage-dependent T-type calcium channel subunit alpha-1I isoform X2
XM_006520949.1	XP_006521012.1	voltage-dependent T-type calcium channel subunit alpha-1I isoform X1
NM_001044308.2	NP_001037773.2	voltage-dependent T-type calcium channel subunit alpha-1I
XM_006520950.1	XP_006521013.1	voltage-dependent T-type calcium channel subunit alpha-1I isoform X2

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	CACNA1I	XP_425474.3
Pan troglodytes (chimpanzee)	CACNA1I	XP_515143.4
Canis lupus familiaris (dog)	CACNA1I	XP_538364.4
Homo sapiens (human)	CACNA1I	NP_001003406.1
Bos taurus (cattle)	CACNA1I	XP_002687981.2
Mus musculus (house mouse)	Cacna1i	NP_001037773.2
Xenopus tropicalis (tropical clawed frog)	cacna1i	XP_002933803.2
Rattus norvegicus (Norway rat)	Cacna1i	NP_064469.3
Danio rerio (zebrafish)	cacna1i	XP_694715.5

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Growth differentiation factor-15 promotes glutamate release in medial prefrontal cortex of mice through upregulation of T-type calcium channels.
Liu DD, Lu JM, Zhao QR, Hu C, Mei YA
Scientific reports628653(2016 Jun)

Contribution of postsynaptic T-type calcium channels to parallel fibre-Purkinje cell synaptic responses.
Ly R, Bouvier G, Szapiro G, Prosser HM, Randall AD, Kano M, Sakimura K, Isope P, Barbour B, Feltz A
The Journal of physiology594(4)915-36(2016 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Extracellular pressure increases [Ca(2+)]i via Cav3.3, driving a PKC-beta- IKK- IkB-NF-kB pathway that stimulates cancer cell proliferation
Title: Increased extracellular pressure stimulates tumor proliferation by a mechanosensitive calcium channel and PKC-?.

using Ca(V)3.3(-/-) mice we found that Ca(V)3.3 channels trigger synaptic plasticity in reticular thalamic neurons
Title: Synaptic plasticity at intrathalamic connections via CaV3.3 T-type Ca2+ channels and GluN2B-containing NMDA receptors.

data identify a central role for Ca(V)3.3 channels in the rhythmogenic properties of the sleep-spindle generator and provide a molecular target to elucidate the roles of sleep spindles for brain function and development.
Title: The Ca(V)3.3 calcium channel is the major sleep spindle pacemaker in thalamus.

alpha1(G) T-type calcium channels contribute to sleep waves as well as to behavioral state of sleep.
Title: T-type calcium channels and thalamocortical rhythms in sleep: a perspective from studies of T-type calcium channel knockout mice.

A T-type calcium channel required for normal function of a mammalian mechanoreceptor.
Title: A T-type calcium channel required for normal function of a mammalian mechanoreceptor.

The following Cacna1i gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cacna1i cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu51299	XM_006520949.2 Latest version!	Mus musculus calcium channel, voltage-dependent, alpha 1I subunit (Cacna1i), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu51300	XM_006520950.2 Latest version!	Mus musculus calcium channel, voltage-dependent, alpha 1I subunit (Cacna1i), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu86721	XM_030248558.1 Latest version!	Mus musculus calcium channel, voltage-dependent, alpha 1I subunit (Cacna1i), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu21028	NM_001044308.2 Latest version!	Mus musculus calcium channel, voltage-dependent, alpha 1I subunit (Cacna1i), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1616.30 $2309.00
View Old Accession Versions >>
OMu51299	XM_006520949.1	Mus musculus calcium channel, voltage-dependent, alpha 1I subunit (Cacna1i), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu51300	XM_006520950.1	Mus musculus calcium channel, voltage-dependent, alpha 1I subunit (Cacna1i), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cacna1i ( XM_006520949.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006521012.1 Mus musculus calcium channel, voltage-dependent, alpha 1I subunit (Cacna1i), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu51299
Clone ID Related Accession (Same CDS sequence)	XM_006520949.2 , XM_006520949.1
Accession Version	XM_006520949.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1I isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006520949.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGACA GCAACTTACC ACCCTCATCT TCAGCAGCCC CGGACCCTGA GCCGGGAATC 
ACTGAGCAGC CGGGGCCCCG GAGTCCCCCT CCATCCCCTC CAGGCCTGGA GGAGCCATTG 
GATGGAACCA ACCCTGACGT CCCGCATCCA GACCTGGCAC CCGTTGCCTT CTTCTGCCTG 
CGCCAGACCA CGAGCCCACG GAACTGGTGC ATCAAGATGG TATGTAACCC GTGGTTCGAG 
TGTGTGAGCA TGCTGGTCAT CCTGCTGAAC TGTGTGACCC TGGGCATGTA CCAGCCATGT 
GACGATATGG AGTGCCTCTC AGACCGCTGC AAGATCCTGC AGGTCTTTGA TGACTTCATC 
TTCATCTTCT TCGCCATGGA GATGGTGCTT AAGATGGTGG CCCTGGGCAT TTTTGGCAAG 
AAGTGCTACC TCGGGGACAC ATGGAACCGC TTGGACTTCT TCATTGTCAT GGCAGGGATG 
GTCGAGTACT CTCTGGACCT ACAGAACATC AACCTGTCAG CCATCCGCAC TGTGCGTGTC 
CTGAGGCCTC TCAAAGCCAT CAACCGTGTT CCCAGCATGC GGATTCTGGT GAACCTGCTG 
CTGGACACGC TGCCCATGCT GGGAAACGTG CTCCTGCTCT GTTTCTTCGT CTTCTTCATC 
TTTGGCATCA TCGGCGTGCA GCTCTGGGCA GGCCTGCTGC GGAACCGCTG CTTCCTGGAG 
GAGAACTTCA CCATACAAGG GGATGTGGCC CTGCCCCCTT ACTACCAACC AGAAGAGGAC 
GACGAGATGC CCTTCATCTG CTCCCTGTCG GGGGACAATG GCATTATGGG CTGTCACGAG 
ATCCCTCCAC TCAAGGAGCA GGGCCGAGAG TGCTGCCTGT CCAAAGATGA CATGTACGAC 
TTCGGGGCGG GACGCCAAGA CCTCAACGCC AGCGGCCTCT GCGTCAACTG GAACCGCTAC 
TACAACGTCT GCCGCACGGG CAACGCCAAC CCTCACAAGG GCGCCATCAA CTTTGACAAC 
ATTGGCTATG CCTGGATCGT GATCTTTCAG GTGATCACTC TGGAAGGCTG GGTGGAGATC 
ATGTACTACG TGATGGATGC ACATTCTTTC TACAACTTCA TCTACTTCAT CCTGCTCATC 
ATAGTGGGCT CCTTCTTCAT GATCAACCTG TGCCTCGTTG TCATAGCGAC CCAGTTCTCT 
GAGACCAAGC AACGGGAGCA CCGGCTGATG CTGGAGCAAC GCCAGCGCTA CCTGTCCTCC 
AGCACGGTGG CCAGTTACGC CGAGCCCGGC GACTGCTATG AGGAGATCTT CCAATATGTC 
TGTCACATCC TTCGCAAGGC CAAGCGCCGT GCTCTAGGCC TCTACCAGGC CCTGCAGAAC 
CGGCGCCAGG CCACGGGCCC CGGGACACCA GCCCCTGCCA AGCCTGGGCC CCATGCCAAG 
GAGCCCAGCC ACTGCAAGCT GTGCCCACGA CACAGTCCCC TGGACACCAC TCCCCACACA 
CTGGTGCAGC CCATCTCTGC CATTCTGGCC TCCGATCCCA GCAGCTGCCC TCGCTGCCAG 
CACGAGGCAG GCAGGCGGCC CTCTGGCCTG GGCAGCACTG ACTCGGGCCA GGAAGGCTCA 
GGTTCTGGCG GTTCTGCAGA GGCTGAAGCC AATGGCGATG GACCCCAAAG CAGTGAGGAT 
GGGGTCTCCT CAGGTTTGGG GAAGGAGGAG GAACAGGAGG ACGGGGCAGC CCGACTGTGT 
GGGGATGTGT GGCGAGAGAC ACGGGCCAAG CTGCGGGGCA TTGTGGACAG CAAGTACTTC 
AACAGAGGCA TCATGATGGC TATTCTGGTT AACACTGTCA GCATGGGCAT CGAGCACCAT 
GAACAGCCAG AGGAACTGAC CAACATCCTG GAGATCTGCA ACGTGGTCTT CACCAGCATG 
TTTGCCCTGG AGATGATTCT GAAGCTGGCT GCCTTTGGGC TCTTTGACTA CCTGCGGAAC 
CCTTACAACA TCTTCGACAG CATCATTGTC ATCATCAGCA TCTGGGAAAT CGTGGGGCAG 
GCTGACGGCG GCCTGTCCGT GCTGCGCACC TTCCGGCTGC TGCGGGTGCT GAAGCTGGTG 
CGCTTCATGC CGGCGCTGCG GCGCCAGCTC GTGGTGCTCA TGAAGACCAT GGACAACGTG 
GCCACCTTCT GCATGCTGCT CATGCTCTTC ATTTTCATCT TCAGCATCCT TGGGATGCAT 
ATCTTTGGCT GCAAATTCAG CCTCCGCACG GACACTGGAG ACACAGTCCC TGACAGGAAG 
AACTTCGATT CCTTACTGTG GGCCATCGTC ACCGTGTTTC AGATCCTCAC TCAGGAGGAC 
TGGAACGTTG TCCTGTACAA TGGCATGGCC TCCACCACCC CCTGGGCCTC CCTCTATTTT 
GTTGCCCTCA TGACCTTTGG CAACTATGTT CTCTTCAATC TCCTGGTGGC TATCCTGGTC 
GAGGGCTTCC AGGCTGAGGG TGATGCTAAT CGCTCCTACT CAGACGAGGA CCAGAGCTCA 
TCCAACTTGG AGGAGCTTGA TAAGCTCCCA GAGGGCCTGG ACAGCAGCAG AGATCTCAAG 
CTCTGCCCAA TACCCATGAC ACCCAATGGA CACCTGGACC CTAGTCTCCC TCTGGGTGGA 
CATCTGGGTC CTGCTGGTGC CATGGGTGCT GCCCCCCGCC TCTCACTGCA GCCAGACCCA 
GTGCTGGTGG CCCTAGAATC CCGGAAAAGC AGTGTCATGT CACTGGGCAG GATGAGCTAT 
GATCAGCGAT CTTTGTCCAG CTCCCGGAGC TCCTACTATG GGCCCTGGGG CCGCAGCGGG 
ACCTGGGCCA GCCGCCGCTC CAGCTGGAAC AGCCTGAAAC ACAAGCCACC CTCAGCTGAG 
CACGAGTCCC TGCTCTCCGG GGAGCGTGGT GGTAGTTGCG TCAGGGCCTG TGAAGGCGCC 
CGGGAGGATG CGCCACCTCG CGCTGCACCC CTGCATGCCC CGCACACCCA CCACGCGCAT 
CATGGACCCC ACTTGGCACA CCGTCACCGC CACCACCGTC GGACACTGTC CCTTGATACC 
AGGGACTCTG TTGACCTGGC AGAGCTGGTG CCCGTGGTGG GTGCCCACTC GCGGGCCGCT 
TGGAGGGCGG CAGGCCAGGC CCCTGGGCAC GAGGACTGCA ATGGCAGAAT GCCCAACATA 
GCCAAGGATG TCTTCACCAA GATGGACGAC CGCCGCGACC GTGGGGAGGA CGAGGAGGAG 
ATCGACTACA CCCTGTGTTT CCGCGTCCGC AAGATGATTG ATGTGTACAA GCCTGACTGG 
TGCGAAGTCC GCGAGGACTG GTCGGTCTAC CTCTTCTCCC CTGAGAACAA GTTCCGGATC 
CTGTGTCAGA CCATTATTGC TCACAAGCTT TTTGACTACG TGGTCTTGGC CTTCATCTTC 
CTCAACTGTA TCACCATTGC TCTGGAGAGG CCCCAGATCG AAGCTGGTAG CACTGAGCGC 
ATCTTCCTCA CGGTGTCTAA CTACATCTTC ACAGCCATCT TTGTGGGCGA GATGACACTG 
AAGGTGGTCT CTTTGGGCCT ATACTTCGGT GAGCAAGCAT ACTTGCGTAG CAGCTGGAAC 
GTCCTGGATG GTTTCCTGGT CTTTGTGTCT ATCATCGATA TCGTGGTATC CGTGGCCTCT 
GCAGGGGGAG CCAAGATCCT GGGGGTCCTC CGGGTCCTGC GGCTCCTGCG TACCTTACGT 
CCTTTGAGGG TCATCAGCCG GGCCCCTGGG CTGAAGCTGG TGGTAGAGAC GCTCATCTCC 
TCCCTCAAGC CCATTGGAAA CATTGTCCTC ATCTGCTGCG CCTTCTTCAT CATCTTCGGT 
ATCCTGGGGG TGCAGCTTTT CAAAGGCAAG TTCTATCACT GTCTGGGAGT GGACACCCGA 
AACATCACCA ACCGATCAGA CTGTGTGGCC GCCAACTACC GCTGGGTGCA TCACAAATAC 
AACTTTGATA ACCTGGGCCA GGCTTTGATG TCCCTCTTTG TCTTGGCCTC CAAGGATGGC 
TGGGTGAACA TCATGTATAA TGGATTGGAT GCTGTTGCTG TGGACCAGCA GCCGGTGACA 
AACCACAACC CCTGGATGCT GCTGTACTTC ATCTCCTTCC TGCTCATCGT CAGCTTCTTC 
GTGCTCAACA TGTTCGTGGG AGTGGTCGTG GAGAACTTCC ACAAGTGCCG GCAGCATCAG 
GAGGCTGAGG AGGCACGGCG GCGTGAGGAG AAACGGCTAC GGCGCCTGGA AAAGAAGCGC 
CGTAAGGCTC AGAGGCTGCC CTACTATGCC ACCTACTGTC CCACAAGACT GCTCATCCAC 
TCCATGTGCA CTAGCCACTA CCTGGACATC TTCATCACCT TCATCATCTG CCTCAACGTG 
GTCACCATGT CCCTGGAGCA CTACAACCAG CCTACATCCC TAGAGACAGC CCTCAAGTAC 
TGCAACTACA TGTTCACCAC TGTCTTTGTG CTGGAGGCTG TGCTGAAGCT GGTGGCGTTT 
GGCCTGAGGC GTTTCTTCAA GGACCGATGG AACCAGCTGG ACTTGGCCAT TGTGCTGCTG 
TCTGTCATGG GCATCACACT GGAGGAGATT GAGATCAATG CGGCCCTGCC CATCAACCCC 
ACCATCATCC GTATCATGCG TGTTCTGCGT ATTGCCCGGG TGTTGAAGCT GTTGAAGATG 
GCCACAGGAA TGCGGGCCCT GCTGGACACA GTGGTACAGG CTCTGCCCCA GGTGGGCAAC 
CTGGGCCTCC TCTTCATGCT GCTCTTCTTC ATCTACGCCG CTCTGGGAGT GGAGCTCTTC 
GGAAAGCTGG TCTGCAATGA CGAGAACCCG TGTGAGGGCA TGAGCCGGCA CGCCACCTTC 
GAGAACTTCG GCATGGCCTT CCTCACACTC TTCCAGGTCT CCACAGGCGA TAACTGGAAT 
GGGATTATGA AGGACACTCT GCGAGACTGC ACCCACGACG AGCGCAGCTG CCTGAGCAGC 
CTGCAGTTTG TGTCGCCTCT CTACTTTGTG AGCTTCGTAC TCACAGCGCA GTTTGTGCTC 
ATCAACGTGG TGGTGGCCGT GCTGATGAAA CATCTGGATG ACAGCAACAA GGAGGCCCAG 
GAGGATGCAG AGATGGATGC TGAGATCGAG CTGGAGATGG CCCATGGCCT AGGCCCCGGG 
CCCGGCCCCT GTCCCTGTCC CTGTCCCTGC CCCTGCCCCT GTCCCTGTCC TGGCCCGAGG 
ATGCCCACCA GTTCACCGGG GGCTCCAGGC CGAGGATCAG GAGGGGCAGG CGTTGGAGGC 
GACACTGAGA GCCACCTGTG CCGGCACTGC TATTCTCCAG CCCAGGAGAC CCTGTGGCTG 
GACAGCGTCT CTTTAATCAT CAAGGACTCC TTGGAGGGGG AGCTGACCAT CATTGACAAC 
CTGTCTGGCT CCATCTTCCA CCACTACTCC TCACCTGCCG GCTGTGACAA GTGTCACCAT 
GACAAGCAAG AGGGACCTGT TGCTCAGCGC TCCAGGCCCA GGCTGCCCCA TCCCAGCCCC 
AGGGTGACCC GAGGCCACCA CCTCCCCCCG CAGGTGCAGC TGGCTGAGAC AGAGGCCTTC 
TCCCTGAACT CAGACAGGTC CTCGTCCGTC CTGCTGGGGG ATGACCTGAG TCTTGAGGAC 
CCCACGGCCT GCCCGCAGGG CCCCAAGGAG AGCAAGGGTG AGCTAGAGCC TCCGGAGCCC 
ATGCAGGCTG GAGACCTGGA TGAATGCTTC TTCCCCTTTG CCGGTGAGCC AGTGTCTGCA 
GGCCCAGAGA GCCTGCTGTG CGAGATGGGG GCCATTCCAT TCAACCCCGT CCAGTCCTGG 
CTCAAACATG AGAGCAACCA AGCACCCCCA AGCCCTTTCT CCCCGGATGG TTCCAGCCCT 
CTGCTGCAGA TGCCTGCTGA GTTCTTCCAC CCTGCTGTGT CTGCCAGCCA GAAGGGGCAG 
GAACCGGGCA TGAGTTCAGG GACCCTGCCC AAGATTGCAC TTCAGGGGTC CTGGGCATCC 
CTGAGGTCAC CGAGTGTCAA CTGCACCCTC TTGCGCCAGG CTACTGTGAG TGACACGTCT 
TTGGATGCCA GCCCTAGCAG CTCAGCGGGC AGCCTGCAGA CCACACTGGA GGACAGTCTG 
ACCCTGAGTG ACAGTCCTCG GCGTGCCCTG GGACCACCAG TCCAGGTGCC AGGGCCACGG 
GCTAGCCTGT CACCTGCCAC CCGGCGCCGC CTCAGCCTGC GGGGGCGTGG CCTGTTTAGT 
CTGCGTGGGC TGCGGGCCCA TCAGCGTAGC CACAGCAGCG GTGGCTCCAC CAGCCCTGGC 
TGCACTTACC ACGACTCCAT GGACCCCTCT GATGAGGAGG GCCGCGGGGG CGCAGGTGGT 
GGGGGCGCGG GCAGTGAGCA CTCCGAGACC CTCAGCAGCC TGTCACTCAC ATCTCTCTTC 
TGCCTACCAC CCACGCTGCC ACCACCCGGT CTCACCCCAG CCAGGAAGTT CAGCAGTACC 
AGCAGCCTGG CTGCTGGACC TGGCCGTCCT GGCGCCACTG TCTCGGTCCG CGGCCTGGCC 
AGGAGTCCCT CATGGGCTGC AGACCGCAGC AAGGACCCAC CAGGCCAGGC ACAGCTGGCC 
TCCGGCTTCG GCTCCTCAGC ACCCGAGCCA CAGCCACCTC CCGGGGAGTC AACGGATGCG 
GCGAGCAAAA GGAAGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006521012.1
CDS	557..7237
Translation	MADSNLPPSSSAAPDPEPGITEQPGPRSPPPSPPGLEEPLDGTNPDVPHPDLAPVAFFCLRQTTSPRNWCIKMVCNPWFECVSMLVILLNCVTLGMYQPCDDMECLSDRCKILQVFDDFIFIFFAMEMVLKMVALGIFGKKCYLGDTWNRLDFFIVMAGMVEYSLDLQNINLSAIRTVRVLRPLKAINRVPSMRILVNLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLWAGLLRNRCFLEENFTIQGDVALPPYYQPEEDDEMPFICSLSGDNGIMGCHEIPPLKEQGRECCLSKDDMYDFGAGRQDLNASGLCVNWNRYYNVCRTGNANPHKGAINFDNIGYAWIVIFQVITLEGWVEIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQREHRLMLEQRQRYLSSSTVASYAEPGDCYEEIFQYVCHILRKAKRRALGLYQALQNRRQATGPGTPAPAKPGPHAKEPSHCKLCPRHSPLDTTPHTLVQPISAILASDPSSCPRCQHEAGRRPSGLGSTDSGQEGSGSGGSAEAEANGDGPQSSEDGVSSGLGKEEEQEDGAARLCGDVWRETRAKLRGIVDSKYFNRGIMMAILVNTVSMGIEHHEQPEELTNILEICNVVFTSMFALEMILKLAAFGLFDYLRNPYNIFDSIIVIISIWEIVGQADGGLSVLRTFRLLRVLKLVRFMPALRRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHIFGCKFSLRTDTGDTVPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMASTTPWASLYFVALMTFGNYVLFNLLVAILVEGFQAEGDANRSYSDEDQSSSNLEELDKLPEGLDSSRDLKLCPIPMTPNGHLDPSLPLGGHLGPAGAMGAAPRLSLQPDPVLVALESRKSSVMSLGRMSYDQRSLSSSRSSYYGPWGRSGTWASRRSSWNSLKHKPPSAEHESLLSGERGGSCVRACEGAREDAPPRAAPLHAPHTHHAHHGPHLAHRHRHHRRTLSLDTRDSVDLAELVPVVGAHSRAAWRAAGQAPGHEDCNGRMPNIAKDVFTKMDDRRDRGEDEEEIDYTLCFRVRKMIDVYKPDWCEVREDWSVYLFSPENKFRILCQTIIAHKLFDYVVLAFIFLNCITIALERPQIEAGSTERIFLTVSNYIFTAIFVGEMTLKVVSLGLYFGEQAYLRSSWNVLDGFLVFVSIIDIVVSVASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLKPIGNIVLICCAFFIIFGILGVQLFKGKFYHCLGVDTRNITNRSDCVAANYRWVHHKYNFDNLGQALMSLFVLASKDGWVNIMYNGLDAVAVDQQPVTNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEAEEARRREEKRLRRLEKKRRKAQRLPYYATYCPTRLLIHSMCTSHYLDIFITFIICLNVVTMSLEHYNQPTSLETALKYCNYMFTTVFVLEAVLKLVAFGLRRFFKDRWNQLDLAIVLLSVMGITLEEIEINAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGKLVCNDENPCEGMSRHATFENFGMAFLTLFQVSTGDNWNGIMKDTLRDCTHDERSCLSSLQFVSPLYFVSFVLTAQFVLINVVVAVLMKHLDDSNKEAQEDAEMDAEIELEMAHGLGPGPGPCPCPCPCPCPCPCPGPRMPTSSPGAPGRGSGGAGVGGDTESHLCRHCYSPAQETLWLDSVSLIIKDSLEGELTIIDNLSGSIFHHYSSPAGCDKCHHDKQEGPVAQRSRPRLPHPSPRVTRGHHLPPQVQLAETEAFSLNSDRSSSVLLGDDLSLEDPTACPQGPKESKGELEPPEPMQAGDLDECFFPFAGEPVSAGPESLLCEMGAIPFNPVQSWLKHESNQAPPSPFSPDGSSPLLQMPAEFFHPAVSASQKGQEPGMSSGTLPKIALQGSWASLRSPSVNCTLLRQATVSDTSLDASPSSSAGSLQTTLEDSLTLSDSPRRALGPPVQVPGPRASLSPATRRRLSLRGRGLFSLRGLRAHQRSHSSGGSTSPGCTYHDSMDPSDEEGRGGAGGGGAGSEHSETLSSLSLTSLFCLPPTLPPPGLTPARKFSSTSSLAAGPGRPGATVSVRGLARSPSWAADRSKDPPGQAQLASGFGSSAPEPQPPPGESTDAASKRKR

Target ORF information:

RefSeq Version	XM_006520949.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, alpha 1I subunit (Cacna1i), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006520949.2

ORF Insert Sequence:

ATGGCTGACA GCAACTTACC ACCCTCATCT TCAGCAGCCC CGGACCCTGA GCCGGGAATC 
ACTGAGCAGC CGGGGCCCCG GAGTCCCCCT CCATCCCCTC CAGGCCTGGA GGAGCCATTG 
GATGGAACCA ACCCTGACGT CCCGCATCCA GACCTGGCAC CCGTTGCCTT CTTCTGCCTG 
CGCCAGACCA CGAGCCCACG GAACTGGTGC ATCAAGATGG TATGTAACCC GTGGTTCGAG 
TGTGTGAGCA TGCTGGTCAT CCTGCTGAAC TGTGTGACCC TGGGCATGTA CCAGCCATGT 
GACGATATGG AGTGCCTCTC AGACCGCTGC AAGATCCTGC AGGTCTTTGA TGACTTCATC 
TTCATCTTCT TCGCCATGGA GATGGTGCTT AAGATGGTGG CCCTGGGCAT TTTTGGCAAG 
AAGTGCTACC TCGGGGACAC ATGGAACCGC TTGGACTTCT TCATTGTCAT GGCAGGGATG 
GTCGAGTACT CTCTGGACCT ACAGAACATC AACCTGTCAG CCATCCGCAC TGTGCGTGTC 
CTGAGGCCTC TCAAAGCCAT CAACCGTGTT CCCAGCATGC GGATTCTGGT GAACCTGCTG 
CTGGACACGC TGCCCATGCT GGGAAACGTG CTCCTGCTCT GTTTCTTCGT CTTCTTCATC 
TTTGGCATCA TCGGCGTGCA GCTCTGGGCA GGCCTGCTGC GGAACCGCTG CTTCCTGGAG 
GAGAACTTCA CCATACAAGG GGATGTGGCC CTGCCCCCTT ACTACCAACC AGAAGAGGAC 
GACGAGATGC CCTTCATCTG CTCCCTGTCG GGGGACAATG GCATTATGGG CTGTCACGAG 
ATCCCTCCAC TCAAGGAGCA GGGCCGAGAG TGCTGCCTGT CCAAAGATGA CATGTACGAC 
TTCGGGGCGG GACGCCAAGA CCTCAACGCC AGCGGCCTCT GCGTCAACTG GAACCGCTAC 
TACAACGTCT GCCGCACGGG CAACGCCAAC CCTCACAAGG GCGCCATCAA CTTTGACAAC 
ATTGGCTATG CCTGGATCGT GATCTTTCAG GTGATCACTC TGGAAGGCTG GGTGGAGATC 
ATGTACTACG TGATGGATGC ACATTCTTTC TACAACTTCA TCTACTTCAT CCTGCTCATC 
ATAGTGGGCT CCTTCTTCAT GATCAACCTG TGCCTCGTTG TCATAGCGAC CCAGTTCTCT 
GAGACCAAGC AACGGGAGCA CCGGCTGATG CTGGAGCAAC GCCAGCGCTA CCTGTCCTCC 
AGCACGGTGG CCAGTTACGC CGAGCCCGGC GACTGCTATG AGGAGATCTT CCAATATGTC 
TGTCACATCC TTCGCAAGGC CAAGCGCCGT GCTCTAGGCC TCTACCAGGC CCTGCAGAAC 
CGGCGCCAGG CCACGGGCCC CGGGACACCA GCCCCTGCCA AGCCTGGGCC CCATGCCAAG 
GAGCCCAGCC ACTGCAAGCT GTGCCCACGA CACAGTCCCC TGGACACCAC TCCCCACACA 
CTGGTGCAGC CCATCTCTGC CATTCTGGCC TCCGATCCCA GCAGCTGCCC TCGCTGCCAG 
CACGAGGCAG GCAGGCGGCC CTCTGGCCTG GGCAGCACTG ACTCGGGCCA GGAAGGCTCA 
GGTTCTGGCG GTTCTGCAGA GGCTGAAGCC AATGGCGATG GACCCCAAAG CAGTGAGGAT 
GGGGTCTCCT CAGGTTTGGG GAAGGAGGAG GAACAGGAGG ACGGGGCAGC CCGACTGTGT 
GGGGATGTGT GGCGAGAGAC ACGGGCCAAG CTGCGGGGCA TTGTGGACAG CAAGTACTTC 
AACAGAGGCA TCATGATGGC TATTCTGGTT AACACTGTCA GCATGGGCAT CGAGCACCAT 
GAACAGCCAG AGGAACTGAC CAACATCCTG GAGATCTGCA ACGTGGTCTT CACCAGCATG 
TTTGCCCTGG AGATGATTCT GAAGCTGGCT GCCTTTGGGC TCTTTGACTA CCTGCGGAAC 
CCTTACAACA TCTTCGACAG CATCATTGTC ATCATCAGCA TCTGGGAAAT CGTGGGGCAG 
GCTGACGGCG GCCTGTCCGT GCTGCGCACC TTCCGGCTGC TGCGGGTGCT GAAGCTGGTG 
CGCTTCATGC CGGCGCTGCG GCGCCAGCTC GTGGTGCTCA TGAAGACCAT GGACAACGTG 
GCCACCTTCT GCATGCTGCT CATGCTCTTC ATTTTCATCT TCAGCATCCT TGGGATGCAT 
ATCTTTGGCT GCAAATTCAG CCTCCGCACG GACACTGGAG ACACAGTCCC TGACAGGAAG 
AACTTCGATT CCTTACTGTG GGCCATCGTC ACCGTGTTTC AGATCCTCAC TCAGGAGGAC 
TGGAACGTTG TCCTGTACAA TGGCATGGCC TCCACCACCC CCTGGGCCTC CCTCTATTTT 
GTTGCCCTCA TGACCTTTGG CAACTATGTT CTCTTCAATC TCCTGGTGGC TATCCTGGTC 
GAGGGCTTCC AGGCTGAGGG TGATGCTAAT CGCTCCTACT CAGACGAGGA CCAGAGCTCA 
TCCAACTTGG AGGAGCTTGA TAAGCTCCCA GAGGGCCTGG ACAGCAGCAG AGATCTCAAG 
CTCTGCCCAA TACCCATGAC ACCCAATGGA CACCTGGACC CTAGTCTCCC TCTGGGTGGA 
CATCTGGGTC CTGCTGGTGC CATGGGTGCT GCCCCCCGCC TCTCACTGCA GCCAGACCCA 
GTGCTGGTGG CCCTAGAATC CCGGAAAAGC AGTGTCATGT CACTGGGCAG GATGAGCTAT 
GATCAGCGAT CTTTGTCCAG CTCCCGGAGC TCCTACTATG GGCCCTGGGG CCGCAGCGGG 
ACCTGGGCCA GCCGCCGCTC CAGCTGGAAC AGCCTGAAAC ACAAGCCACC CTCAGCTGAG 
CACGAGTCCC TGCTCTCCGG GGAGCGTGGT GGTAGTTGCG TCAGGGCCTG TGAAGGCGCC 
CGGGAGGATG CGCCACCTCG CGCTGCACCC CTGCATGCCC CGCACACCCA CCACGCGCAT 
CATGGACCCC ACTTGGCACA CCGTCACCGC CACCACCGTC GGACACTGTC CCTTGATACC 
AGGGACTCTG TTGACCTGGC AGAGCTGGTG CCCGTGGTGG GTGCCCACTC GCGGGCCGCT 
TGGAGGGCGG CAGGCCAGGC CCCTGGGCAC GAGGACTGCA ATGGCAGAAT GCCCAACATA 
GCCAAGGATG TCTTCACCAA GATGGACGAC CGCCGCGACC GTGGGGAGGA CGAGGAGGAG 
ATCGACTACA CCCTGTGTTT CCGCGTCCGC AAGATGATTG ATGTGTACAA GCCTGACTGG 
TGCGAAGTCC GCGAGGACTG GTCGGTCTAC CTCTTCTCCC CTGAGAACAA GTTCCGGATC 
CTGTGTCAGA CCATTATTGC TCACAAGCTT TTTGACTACG TGGTCTTGGC CTTCATCTTC 
CTCAACTGTA TCACCATTGC TCTGGAGAGG CCCCAGATCG AAGCTGGTAG CACTGAGCGC 
ATCTTCCTCA CGGTGTCTAA CTACATCTTC ACAGCCATCT TTGTGGGCGA GATGACACTG 
AAGGTGGTCT CTTTGGGCCT ATACTTCGGT GAGCAAGCAT ACTTGCGTAG CAGCTGGAAC 
GTCCTGGATG GTTTCCTGGT CTTTGTGTCT ATCATCGATA TCGTGGTATC CGTGGCCTCT 
GCAGGGGGAG CCAAGATCCT GGGGGTCCTC CGGGTCCTGC GGCTCCTGCG TACCTTACGT 
CCTTTGAGGG TCATCAGCCG GGCCCCTGGG CTGAAGCTGG TGGTAGAGAC GCTCATCTCC 
TCCCTCAAGC CCATTGGAAA CATTGTCCTC ATCTGCTGCG CCTTCTTCAT CATCTTCGGT 
ATCCTGGGGG TGCAGCTTTT CAAAGGCAAG TTCTATCACT GTCTGGGAGT GGACACCCGA 
AACATCACCA ACCGATCAGA CTGTGTGGCC GCCAACTACC GCTGGGTGCA TCACAAATAC 
AACTTTGATA ACCTGGGCCA GGCTTTGATG TCCCTCTTTG TCTTGGCCTC CAAGGATGGC 
TGGGTGAACA TCATGTATAA TGGATTGGAT GCTGTTGCTG TGGACCAGCA GCCGGTGACA 
AACCACAACC CCTGGATGCT GCTGTACTTC ATCTCCTTCC TGCTCATCGT CAGCTTCTTC 
GTGCTCAACA TGTTCGTGGG AGTGGTCGTG GAGAACTTCC ACAAGTGCCG GCAGCATCAG 
GAGGCTGAGG AGGCACGGCG GCGTGAGGAG AAACGGCTAC GGCGCCTGGA AAAGAAGCGC 
CGTAAGGCTC AGAGGCTGCC CTACTATGCC ACCTACTGTC CCACAAGACT GCTCATCCAC 
TCCATGTGCA CTAGCCACTA CCTGGACATC TTCATCACCT TCATCATCTG CCTCAACGTG 
GTCACCATGT CCCTGGAGCA CTACAACCAG CCTACATCCC TAGAGACAGC CCTCAAGTAC 
TGCAACTACA TGTTCACCAC TGTCTTTGTG CTGGAGGCTG TGCTGAAGCT GGTGGCGTTT 
GGCCTGAGGC GTTTCTTCAA GGACCGATGG AACCAGCTGG ACTTGGCCAT TGTGCTGCTG 
TCTGTCATGG GCATCACACT GGAGGAGATT GAGATCAATG CGGCCCTGCC CATCAACCCC 
ACCATCATCC GTATCATGCG TGTTCTGCGT ATTGCCCGGG TGTTGAAGCT GTTGAAGATG 
GCCACAGGAA TGCGGGCCCT GCTGGACACA GTGGTACAGG CTCTGCCCCA GGTGGGCAAC 
CTGGGCCTCC TCTTCATGCT GCTCTTCTTC ATCTACGCCG CTCTGGGAGT GGAGCTCTTC 
GGAAAGCTGG TCTGCAATGA CGAGAACCCG TGTGAGGGCA TGAGCCGGCA CGCCACCTTC 
GAGAACTTCG GCATGGCCTT CCTCACACTC TTCCAGGTCT CCACAGGCGA TAACTGGAAT 
GGGATTATGA AGGACACTCT GCGAGACTGC ACCCACGACG AGCGCAGCTG CCTGAGCAGC 
CTGCAGTTTG TGTCGCCTCT CTACTTTGTG AGCTTCGTAC TCACAGCGCA GTTTGTGCTC 
ATCAACGTGG TGGTGGCCGT GCTGATGAAA CATCTGGATG ACAGCAACAA GGAGGCCCAG 
GAGGATGCAG AGATGGATGC TGAGATCGAG CTGGAGATGG CCCATGGCCT AGGCCCCGGG 
CCCGGCCCCT GTCCCTGTCC CTGTCCCTGC CCCTGCCCCT GTCCCTGTCC TGGCCCGAGG 
ATGCCCACCA GTTCACCGGG GGCTCCAGGC CGAGGATCAG GAGGGGCAGG CGTTGGAGGC 
GACACTGAGA GCCACCTGTG CCGGCACTGC TATTCTCCAG CCCAGGAGAC CCTGTGGCTG 
GACAGCGTCT CTTTAATCAT CAAGGACTCC TTGGAGGGGG AGCTGACCAT CATTGACAAC 
CTGTCTGGCT CCATCTTCCA CCACTACTCC TCACCTGCCG GCTGTGACAA GTGTCACCAT 
GACAAGCAAG AGGGACCTGT TGCTCAGCGC TCCAGGCCCA GGCTGCCCCA TCCCAGCCCC 
AGGGTGACCC GAGGCCACCA CCTCCCCCCG CAGGTGCAGC TGGCTGAGAC AGAGGCCTTC 
TCCCTGAACT CAGACAGGTC CTCGTCCGTC CTGCTGGGGG ATGACCTGAG TCTTGAGGAC 
CCCACGGCCT GCCCGCAGGG CCCCAAGGAG AGCAAGGGTG AGCTAGAGCC TCCGGAGCCC 
ATGCAGGCTG GAGACCTGGA TGAATGCTTC TTCCCCTTTG CCGGTGAGCC AGTGTCTGCA 
GGCCCAGAGA GCCTGCTGTG CGAGATGGGG GCCATTCCAT TCAACCCCGT CCAGTCCTGG 
CTCAAACATG AGAGCAACCA AGCACCCCCA AGCCCTTTCT CCCCGGATGG TTCCAGCCCT 
CTGCTGCAGA TGCCTGCTGA GTTCTTCCAC CCTGCTGTGT CTGCCAGCCA GAAGGGGCAG 
GAACCGGGCA TGAGTTCAGG GACCCTGCCC AAGATTGCAC TTCAGGGGTC CTGGGCATCC 
CTGAGGTCAC CGAGTGTCAA CTGCACCCTC TTGCGCCAGG CTACTGTGAG TGACACGTCT 
TTGGATGCCA GCCCTAGCAG CTCAGCGGGC AGCCTGCAGA CCACACTGGA GGACAGTCTG 
ACCCTGAGTG ACAGTCCTCG GCGTGCCCTG GGACCACCAG TCCAGGTGCC AGGGCCACGG 
GCTAGCCTGT CACCTGCCAC CCGGCGCCGC CTCAGCCTGC GGGGGCGTGG CCTGTTTAGT 
CTGCGTGGGC TGCGGGCCCA TCAGCGTAGC CACAGCAGCG GTGGCTCCAC CAGCCCTGGC 
TGCACTTACC ACGACTCCAT GGACCCCTCT GATGAGGAGG GCCGCGGGGG CGCAGGTGGT 
GGGGGCGCGG GCAGTGAGCA CTCCGAGACC CTCAGCAGCC TGTCACTCAC ATCTCTCTTC 
TGCCTACCAC CCACGCTGCC ACCACCCGGT CTCACCCCAG CCAGGAAGTT CAGCAGTACC 
AGCAGCCTGG CTGCTGGACC TGGCCGTCCT GGCGCCACTG TCTCGGTCCG CGGCCTGGCC 
AGGAGTCCCT CATGGGCTGC AGACCGCAGC AAGGACCCAC CAGGCCAGGC ACAGCTGGCC 
TCCGGCTTCG GCTCCTCAGC ACCCGAGCCA CAGCCACCTC CCGGGGAGTC AACGGATGCG 
GCGAGCAAAA GGAAGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1i ( XM_006520950.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006521013.1 Mus musculus calcium channel, voltage-dependent, alpha 1I subunit (Cacna1i), transcript variant X2, mRNA.

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CloneID	OMu51300
Clone ID Related Accession (Same CDS sequence)	XM_006520950.2 , XM_006520950.1
Accession Version	XM_006520950.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1I isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006520950.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGACA GCAACTTACC ACCCTCATCT TCAGCAGCCC CGGACCCTGA GCCGGGAATC 
ACTGAGCAGC CGGGGCCCCG GAGTCCCCCT CCATCCCCTC CAGGCCTGGA GGAGCCATTG 
GATGGAACCA ACCCTGACGT CCCGCATCCA GACCTGGCAC CCGTTGCCTT CTTCTGCCTG 
CGCCAGACCA CGAGCCCACG GAACTGGTGC ATCAAGATGG TATGTAACCC GTGGTTCGAG 
TGTGTGAGCA TGCTGGTCAT CCTGCTGAAC TGTGTGACCC TGGGCATGTA CCAGCCATGT 
GACGATATGG AGTGCCTCTC AGACCGCTGC AAGATCCTGC AGGTCTTTGA TGACTTCATC 
TTCATCTTCT TCGCCATGGA GATGGTGCTT AAGATGGTGG CCCTGGGCAT TTTTGGCAAG 
AAGTGCTACC TCGGGGACAC ATGGAACCGC TTGGACTTCT TCATTGTCAT GGCAGGGATG 
GTCGAGTACT CTCTGGACCT ACAGAACATC AACCTGTCAG CCATCCGCAC TGTGCGTGTC 
CTGAGGCCTC TCAAAGCCAT CAACCGTGTT CCCAGCATGC GGATTCTGGT GAACCTGCTG 
CTGGACACGC TGCCCATGCT GGGAAACGTG CTCCTGCTCT GTTTCTTCGT CTTCTTCATC 
TTTGGCATCA TCGGCGTGCA GCTCTGGGCA GGCCTGCTGC GGAACCGCTG CTTCCTGGAG 
GAGAACTTCA CCATACAAGG GGATGTGGCC CTGCCCCCTT ACTACCAACC AGAAGAGGAC 
GACGAGATGC CCTTCATCTG CTCCCTGTCG GGGGACAATG GCATTATGGG CTGTCACGAG 
ATCCCTCCAC TCAAGGAGCA GGGCCGAGAG TGCTGCCTGT CCAAAGATGA CATGTACGAC 
TTCGGGGCGG GACGCCAAGA CCTCAACGCC AGCGGCCTCT GCGTCAACTG GAACCGCTAC 
TACAACGTCT GCCGCACGGG CAACGCCAAC CCTCACAAGG GCGCCATCAA CTTTGACAAC 
ATTGGCTATG CCTGGATCGT GATCTTTCAG GTGATCACTC TGGAAGGCTG GGTGGAGATC 
ATGTACTACG TGATGGATGC ACATTCTTTC TACAACTTCA TCTACTTCAT CCTGCTCATC 
ATAGTGGGCT CCTTCTTCAT GATCAACCTG TGCCTCGTTG TCATAGCGAC CCAGTTCTCT 
GAGACCAAGC AACGGGAGCA CCGGCTGATG CTGGAGCAAC GCCAGCGCTA CCTGTCCTCC 
AGCACGGTGG CCAGTTACGC CGAGCCCGGC GACTGCTATG AGGAGATCTT CCAATATGTC 
TGTCACATCC TTCGCAAGGC CAAGCGCCGT GCTCTAGGCC TCTACCAGGC CCTGCAGAAC 
CGGCGCCAGG CCACGGGCCC CGGGACACCA GCCCCTGCCA AGCCTGGGCC CCATGCCAAG 
GAGCCCAGCC ACTGCAAGCT GTGCCCACGA CACAGTCCCC TGGACACCAC TCCCCACACA 
CTGGTGCAGC CCATCTCTGC CATTCTGGCC TCCGATCCCA GCAGCTGCCC TCGCTGCCAG 
CACGAGGCAG GCAGGCGGCC CTCTGGCCTG GGCAGCACTG ACTCGGGCCA GGAAGGCTCA 
GGTTCTGGCG GTTCTGCAGA GGCTGAAGCC AATGGCGATG GACCCCAAAG CAGTGAGGAT 
GGGGTCTCCT CAGGTTTGGG GAAGGAGGAG GAACAGGAGG ACGGGGCAGC CCGACTGTGT 
GGGGATGTGT GGCGAGAGAC ACGGGCCAAG CTGCGGGGCA TTGTGGACAG CAAGTACTTC 
AACAGAGGCA TCATGATGGC TATTCTGGTT AACACTGTCA GCATGGGCAT CGAGCACCAT 
GAACAGCCAG AGGAACTGAC CAACATCCTG GAGATCTGCA ACGTGGTCTT CACCAGCATG 
TTTGCCCTGG AGATGATTCT GAAGCTGGCT GCCTTTGGGC TCTTTGACTA CCTGCGGAAC 
CCTTACAACA TCTTCGACAG CATCATTGTC ATCATCAGCA TCTGGGAAAT CGTGGGGCAG 
GCTGACGGCG GCCTGTCCGT GCTGCGCACC TTCCGGCTGC TGCGGGTGCT GAAGCTGGTG 
CGCTTCATGC CGGCGCTGCG GCGCCAGCTC GTGGTGCTCA TGAAGACCAT GGACAACGTG 
GCCACCTTCT GCATGCTGCT CATGCTCTTC ATTTTCATCT TCAGCATCCT TGGGATGCAT 
ATCTTTGGCT GCAAATTCAG CCTCCGCACG GACACTGGAG ACACAGTCCC TGACAGGAAG 
AACTTCGATT CCTTACTGTG GGCCATCGTC ACCGTGTTTC AGATCCTCAC TCAGGAGGAC 
TGGAACGTTG TCCTGTACAA TGGCATGGCC TCCACCACCC CCTGGGCCTC CCTCTATTTT 
GTTGCCCTCA TGACCTTTGG CAACTATGTT CTCTTCAATC TCCTGGTGGC TATCCTGGTC 
GAGGGCTTCC AGGCTGAGGG TGATGCTAAT CGCTCCTACT CAGACGAGGA CCAGAGCTCA 
TCCAACTTGG AGGAGCTTGA TAAGCTCCCA GAGGGCCTGG ACAGCAGCAG AGATCTCAAG 
CTCTGCCCAA TACCCATGAC ACCCAATGGA CACCTGGACC CTAGTCTCCC TCTGGGTGGA 
CATCTGGGTC CTGCTGGTGC CATGGGTGCT GCCCCCCGCC TCTCACTGCA GCCAGACCCA 
GTGCTGGTGG CCCTAGAATC CCGGAAAAGC AGTGTCATGT CACTGGGCAG GATGAGCTAT 
GATCAGCGAT CTTTGTCCAG CTCCCGGAGC TCCTACTATG GGCCCTGGGG CCGCAGCGGG 
ACCTGGGCCA GCCGCCGCTC CAGCTGGAAC AGCCTGAAAC ACAAGCCACC CTCAGCTGAG 
CACGAGTCCC TGCTCTCCGG GGAGCGTGGT GGTAGTTGCG TCAGGGCCTG TGAAGGCGCC 
CGGGAGGATG CGCCACCTCG CGCTGCACCC CTGCATGCCC CGCACACCCA CCACGCGCAT 
CATGGACCCC ACTTGGCACA CCGTCACCGC CACCACCGTC GGACACTGTC CCTTGATACC 
AGGGACTCTG TTGACCTGGC AGAGCTGGTG CCCGTGGTGG GTGCCCACTC GCGGGCCGCT 
TGGAGGGCGG CAGGCCAGGC CCCTGGGCAC GAGGACTGCA ATGGCAGAAT GCCCAACATA 
GCCAAGGATG TCTTCACCAA GATGGACGAC CGCCGCGACC GTGGGGAGGA CGAGGAGGAG 
ATCGACTACA CCCTGTGTTT CCGCGTCCGC AAGATGATTG ATGTGTACAA GCCTGACTGG 
TGCGAAGTCC GCGAGGACTG GTCGGTCTAC CTCTTCTCCC CTGAGAACAA GTTCCGGATC 
CTGTGTCAGA CCATTATTGC TCACAAGCTT TTTGACTACG TGGTCTTGGC CTTCATCTTC 
CTCAACTGTA TCACCATTGC TCTGGAGAGG CCCCAGATCG AAGCTGGTAG CACTGAGCGC 
ATCTTCCTCA CGGTGTCTAA CTACATCTTC ACAGCCATCT TTGTGGGCGA GATGACACTG 
AAGGTGGTCT CTTTGGGCCT ATACTTCGGT GAGCAAGCAT ACTTGCGTAG CAGCTGGAAC 
GTCCTGGATG GTTTCCTGGT CTTTGTGTCT ATCATCGATA TCGTGGTATC CGTGGCCTCT 
GCAGGGGGAG CCAAGATCCT GGGGGTCCTC CGGGTCCTGC GGCTCCTGCG TACCTTACGT 
CCTTTGAGGG TCATCAGCCG GGCCCCTGGG CTGAAGCTGG TGGTAGAGAC GCTCATCTCC 
TCCCTCAAGC CCATTGGAAA CATTGTCCTC ATCTGCTGCG CCTTCTTCAT CATCTTCGGT 
ATCCTGGGGG TGCAGCTTTT CAAAGGCAAG TTCTATCACT GTCTGGGAGT GGACACCCGA 
AACATCACCA ACCGATCAGA CTGTGTGGCC GCCAACTACC GCTGGGTGCA TCACAAATAC 
AACTTTGATA ACCTGGGCCA GGCTTTGATG TCCCTCTTTG TCTTGGCCTC CAAGGATGGC 
TGGGTGAACA TCATGTATAA TGGATTGGAT GCTGTTGCTG TGGACCAGCA GCCGGTGACA 
AACCACAACC CCTGGATGCT GCTGTACTTC ATCTCCTTCC TGCTCATCGT CAGCTTCTTC 
GTGCTCAACA TGTTCGTGGG AGTGGTCGTG GAGAACTTCC ACAAGTGCCG GCAGCATCAG 
GAGGCTGAGG AGGCACGGCG GCGTGAGGAG AAACGGCTAC GGCGCCTGGA AAAGAAGCGC 
CGTAAGGCTC AGAGGCTGCC CTACTATGCC ACCTACTGTC CCACAAGACT GCTCATCCAC 
TCCATGTGCA CTAGCCACTA CCTGGACATC TTCATCACCT TCATCATCTG CCTCAACGTG 
GTCACCATGT CCCTGGAGCA CTACAACCAG CCTACATCCC TAGAGACAGC CCTCAAGTAC 
TGCAACTACA TGTTCACCAC TGTCTTTGTG CTGGAGGCTG TGCTGAAGCT GGTGGCGTTT 
GGCCTGAGGC GTTTCTTCAA GGACCGATGG AACCAGCTGG ACTTGGCCAT TGTGCTGCTG 
TCTGTCATGG GCATCACACT GGAGGAGATT GAGATCAATG CGGCCCTGCC CATCAACCCC 
ACCATCATCC GTATCATGCG TGTTCTGCGT ATTGCCCGGG TGTTGAAGCT GTTGAAGATG 
GCCACAGGAA TGCGGGCCCT GCTGGACACA GTGGTACAGG CTCTGCCCCA GGTGGGCAAC 
CTGGGCCTCC TCTTCATGCT GCTCTTCTTC ATCTACGCCG CTCTGGGAGT GGAGCTCTTC 
GGAAAGCTGG TCTGCAATGA CGAGAACCCG TGTGAGGGCA TGAGCCGGCA CGCCACCTTC 
GAGAACTTCG GCATGGCCTT CCTCACACTC TTCCAGGTCT CCACAGGCGA TAACTGGAAT 
GGGATTATGA AGGACACTCT GCGAGACTGC ACCCACGACG AGCGCAGCTG CCTGAGCAGC 
CTGCAGTTTG TGTCGCCTCT CTACTTTGTG AGCTTCGTAC TCACAGCGCA GTTTGTGCTC 
ATCAACGTGG TGGTGGCCGT GCTGATGAAA CATCTGGATG ACAGCAACAA GGAGGCCCAG 
GAGGATGCAG AGATGGATGC TGAGATCGAG CTGGAGATGG CCCATGGCCT AGGCCCCGGG 
CCCGGCCCCT GTCCCTGTCC CTGTCCCTGC CCCTGCCCCT GTCCCTGTCC TGGCCCGAGG 
ATGCCCACCA GTTCACCGGG GGCTCCAGGC CGAGGATCAG GAGGGGCAGG CGTTGGAGGC 
GACACTGAGA GCCACCTGTG CCGGCACTGC TATTCTCCAG CCCAGGACTC CTTGGAGGGG 
GAGCTGACCA TCATTGACAA CCTGTCTGGC TCCATCTTCC ACCACTACTC CTCACCTGCC 
GGCTGTGACA AGTGTCACCA TGACAAGCAA GAGGGACCTG TTGCTCAGCG CTCCAGGCCC 
AGGCTGCCCC ATCCCAGCCC CAGGGTGACC CGAGGCCACC ACCTCCCCCC GCAGGTGCAG 
CTGGCTGAGA CAGAGGCCTT CTCCCTGAAC TCAGACAGGT CCTCGTCCGT CCTGCTGGGG 
GATGACCTGA GTCTTGAGGA CCCCACGGCC TGCCCGCAGG GCCCCAAGGA GAGCAAGGGT 
GAGCTAGAGC CTCCGGAGCC CATGCAGGCT GGAGACCTGG ATGAATGCTT CTTCCCCTTT 
GCCGGTGAGC CAGTGTCTGC AGGCCCAGAG AGCCTGCTGT GCGAGATGGG GGCCATTCCA 
TTCAACCCCG TCCAGTCCTG GCTCAAACAT GAGAGCAACC AAGCACCCCC AAGCCCTTTC 
TCCCCGGATG GTTCCAGCCC TCTGCTGCAG ATGCCTGCTG AGTTCTTCCA CCCTGCTGTG 
TCTGCCAGCC AGAAGGGGCA GGAACCGGGC ATGAGTTCAG GGACCCTGCC CAAGATTGCA 
CTTCAGGGGT CCTGGGCATC CCTGAGGTCA CCGAGTGTCA ACTGCACCCT CTTGCGCCAG 
GCTACTGTGA GTGACACGTC TTTGGATGCC AGCCCTAGCA GCTCAGCGGG CAGCCTGCAG 
ACCACACTGG AGGACAGTCT GACCCTGAGT GACAGTCCTC GGCGTGCCCT GGGACCACCA 
GTCCAGGTGC CAGGGCCACG GGCTAGCCTG TCACCTGCCA CCCGGCGCCG CCTCAGCCTG 
CGGGGGCGTG GCCTGTTTAG TCTGCGTGGG CTGCGGGCCC ATCAGCGTAG CCACAGCAGC 
GGTGGCTCCA CCAGCCCTGG CTGCACTTAC CACGACTCCA TGGACCCCTC TGATGAGGAG 
GGCCGCGGGG GCGCAGGTGG TGGGGGCGCG GGCAGTGAGC ACTCCGAGAC CCTCAGCAGC 
CTGTCACTCA CATCTCTCTT CTGCCTACCA CCCACGCTGC CACCACCCGG TCTCACCCCA 
GCCAGGAAGT TCAGCAGTAC CAGCAGCCTG GCTGCTGGAC CTGGCCGTCC TGGCGCCACT 
GTCTCGGTCC GCGGCCTGGC CAGGAGTCCC TCATGGGCTG CAGACCGCAG CAAGGACCCA 
CCAGGCCAGG CACAGCTGGC CTCCGGCTTC GGCTCCTCAG CACCCGAGCC ACAGCCACCT 
CCCGGGGAGT CAACGGATGC GGCGAGCAAA AGGAAGAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006521013.1
CDS	557..7198
Translation	MADSNLPPSSSAAPDPEPGITEQPGPRSPPPSPPGLEEPLDGTNPDVPHPDLAPVAFFCLRQTTSPRNWCIKMVCNPWFECVSMLVILLNCVTLGMYQPCDDMECLSDRCKILQVFDDFIFIFFAMEMVLKMVALGIFGKKCYLGDTWNRLDFFIVMAGMVEYSLDLQNINLSAIRTVRVLRPLKAINRVPSMRILVNLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLWAGLLRNRCFLEENFTIQGDVALPPYYQPEEDDEMPFICSLSGDNGIMGCHEIPPLKEQGRECCLSKDDMYDFGAGRQDLNASGLCVNWNRYYNVCRTGNANPHKGAINFDNIGYAWIVIFQVITLEGWVEIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQREHRLMLEQRQRYLSSSTVASYAEPGDCYEEIFQYVCHILRKAKRRALGLYQALQNRRQATGPGTPAPAKPGPHAKEPSHCKLCPRHSPLDTTPHTLVQPISAILASDPSSCPRCQHEAGRRPSGLGSTDSGQEGSGSGGSAEAEANGDGPQSSEDGVSSGLGKEEEQEDGAARLCGDVWRETRAKLRGIVDSKYFNRGIMMAILVNTVSMGIEHHEQPEELTNILEICNVVFTSMFALEMILKLAAFGLFDYLRNPYNIFDSIIVIISIWEIVGQADGGLSVLRTFRLLRVLKLVRFMPALRRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHIFGCKFSLRTDTGDTVPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMASTTPWASLYFVALMTFGNYVLFNLLVAILVEGFQAEGDANRSYSDEDQSSSNLEELDKLPEGLDSSRDLKLCPIPMTPNGHLDPSLPLGGHLGPAGAMGAAPRLSLQPDPVLVALESRKSSVMSLGRMSYDQRSLSSSRSSYYGPWGRSGTWASRRSSWNSLKHKPPSAEHESLLSGERGGSCVRACEGAREDAPPRAAPLHAPHTHHAHHGPHLAHRHRHHRRTLSLDTRDSVDLAELVPVVGAHSRAAWRAAGQAPGHEDCNGRMPNIAKDVFTKMDDRRDRGEDEEEIDYTLCFRVRKMIDVYKPDWCEVREDWSVYLFSPENKFRILCQTIIAHKLFDYVVLAFIFLNCITIALERPQIEAGSTERIFLTVSNYIFTAIFVGEMTLKVVSLGLYFGEQAYLRSSWNVLDGFLVFVSIIDIVVSVASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLKPIGNIVLICCAFFIIFGILGVQLFKGKFYHCLGVDTRNITNRSDCVAANYRWVHHKYNFDNLGQALMSLFVLASKDGWVNIMYNGLDAVAVDQQPVTNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEAEEARRREEKRLRRLEKKRRKAQRLPYYATYCPTRLLIHSMCTSHYLDIFITFIICLNVVTMSLEHYNQPTSLETALKYCNYMFTTVFVLEAVLKLVAFGLRRFFKDRWNQLDLAIVLLSVMGITLEEIEINAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGKLVCNDENPCEGMSRHATFENFGMAFLTLFQVSTGDNWNGIMKDTLRDCTHDERSCLSSLQFVSPLYFVSFVLTAQFVLINVVVAVLMKHLDDSNKEAQEDAEMDAEIELEMAHGLGPGPGPCPCPCPCPCPCPCPGPRMPTSSPGAPGRGSGGAGVGGDTESHLCRHCYSPAQDSLEGELTIIDNLSGSIFHHYSSPAGCDKCHHDKQEGPVAQRSRPRLPHPSPRVTRGHHLPPQVQLAETEAFSLNSDRSSSVLLGDDLSLEDPTACPQGPKESKGELEPPEPMQAGDLDECFFPFAGEPVSAGPESLLCEMGAIPFNPVQSWLKHESNQAPPSPFSPDGSSPLLQMPAEFFHPAVSASQKGQEPGMSSGTLPKIALQGSWASLRSPSVNCTLLRQATVSDTSLDASPSSSAGSLQTTLEDSLTLSDSPRRALGPPVQVPGPRASLSPATRRRLSLRGRGLFSLRGLRAHQRSHSSGGSTSPGCTYHDSMDPSDEEGRGGAGGGGAGSEHSETLSSLSLTSLFCLPPTLPPPGLTPARKFSSTSSLAAGPGRPGATVSVRGLARSPSWAADRSKDPPGQAQLASGFGSSAPEPQPPPGESTDAASKRKR

Target ORF information:

RefSeq Version	XM_006520950.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, alpha 1I subunit (Cacna1i), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006520950.2

ORF Insert Sequence:

ATGGCTGACA GCAACTTACC ACCCTCATCT TCAGCAGCCC CGGACCCTGA GCCGGGAATC 
ACTGAGCAGC CGGGGCCCCG GAGTCCCCCT CCATCCCCTC CAGGCCTGGA GGAGCCATTG 
GATGGAACCA ACCCTGACGT CCCGCATCCA GACCTGGCAC CCGTTGCCTT CTTCTGCCTG 
CGCCAGACCA CGAGCCCACG GAACTGGTGC ATCAAGATGG TATGTAACCC GTGGTTCGAG 
TGTGTGAGCA TGCTGGTCAT CCTGCTGAAC TGTGTGACCC TGGGCATGTA CCAGCCATGT 
GACGATATGG AGTGCCTCTC AGACCGCTGC AAGATCCTGC AGGTCTTTGA TGACTTCATC 
TTCATCTTCT TCGCCATGGA GATGGTGCTT AAGATGGTGG CCCTGGGCAT TTTTGGCAAG 
AAGTGCTACC TCGGGGACAC ATGGAACCGC TTGGACTTCT TCATTGTCAT GGCAGGGATG 
GTCGAGTACT CTCTGGACCT ACAGAACATC AACCTGTCAG CCATCCGCAC TGTGCGTGTC 
CTGAGGCCTC TCAAAGCCAT CAACCGTGTT CCCAGCATGC GGATTCTGGT GAACCTGCTG 
CTGGACACGC TGCCCATGCT GGGAAACGTG CTCCTGCTCT GTTTCTTCGT CTTCTTCATC 
TTTGGCATCA TCGGCGTGCA GCTCTGGGCA GGCCTGCTGC GGAACCGCTG CTTCCTGGAG 
GAGAACTTCA CCATACAAGG GGATGTGGCC CTGCCCCCTT ACTACCAACC AGAAGAGGAC 
GACGAGATGC CCTTCATCTG CTCCCTGTCG GGGGACAATG GCATTATGGG CTGTCACGAG 
ATCCCTCCAC TCAAGGAGCA GGGCCGAGAG TGCTGCCTGT CCAAAGATGA CATGTACGAC 
TTCGGGGCGG GACGCCAAGA CCTCAACGCC AGCGGCCTCT GCGTCAACTG GAACCGCTAC 
TACAACGTCT GCCGCACGGG CAACGCCAAC CCTCACAAGG GCGCCATCAA CTTTGACAAC 
ATTGGCTATG CCTGGATCGT GATCTTTCAG GTGATCACTC TGGAAGGCTG GGTGGAGATC 
ATGTACTACG TGATGGATGC ACATTCTTTC TACAACTTCA TCTACTTCAT CCTGCTCATC 
ATAGTGGGCT CCTTCTTCAT GATCAACCTG TGCCTCGTTG TCATAGCGAC CCAGTTCTCT 
GAGACCAAGC AACGGGAGCA CCGGCTGATG CTGGAGCAAC GCCAGCGCTA CCTGTCCTCC 
AGCACGGTGG CCAGTTACGC CGAGCCCGGC GACTGCTATG AGGAGATCTT CCAATATGTC 
TGTCACATCC TTCGCAAGGC CAAGCGCCGT GCTCTAGGCC TCTACCAGGC CCTGCAGAAC 
CGGCGCCAGG CCACGGGCCC CGGGACACCA GCCCCTGCCA AGCCTGGGCC CCATGCCAAG 
GAGCCCAGCC ACTGCAAGCT GTGCCCACGA CACAGTCCCC TGGACACCAC TCCCCACACA 
CTGGTGCAGC CCATCTCTGC CATTCTGGCC TCCGATCCCA GCAGCTGCCC TCGCTGCCAG 
CACGAGGCAG GCAGGCGGCC CTCTGGCCTG GGCAGCACTG ACTCGGGCCA GGAAGGCTCA 
GGTTCTGGCG GTTCTGCAGA GGCTGAAGCC AATGGCGATG GACCCCAAAG CAGTGAGGAT 
GGGGTCTCCT CAGGTTTGGG GAAGGAGGAG GAACAGGAGG ACGGGGCAGC CCGACTGTGT 
GGGGATGTGT GGCGAGAGAC ACGGGCCAAG CTGCGGGGCA TTGTGGACAG CAAGTACTTC 
AACAGAGGCA TCATGATGGC TATTCTGGTT AACACTGTCA GCATGGGCAT CGAGCACCAT 
GAACAGCCAG AGGAACTGAC CAACATCCTG GAGATCTGCA ACGTGGTCTT CACCAGCATG 
TTTGCCCTGG AGATGATTCT GAAGCTGGCT GCCTTTGGGC TCTTTGACTA CCTGCGGAAC 
CCTTACAACA TCTTCGACAG CATCATTGTC ATCATCAGCA TCTGGGAAAT CGTGGGGCAG 
GCTGACGGCG GCCTGTCCGT GCTGCGCACC TTCCGGCTGC TGCGGGTGCT GAAGCTGGTG 
CGCTTCATGC CGGCGCTGCG GCGCCAGCTC GTGGTGCTCA TGAAGACCAT GGACAACGTG 
GCCACCTTCT GCATGCTGCT CATGCTCTTC ATTTTCATCT TCAGCATCCT TGGGATGCAT 
ATCTTTGGCT GCAAATTCAG CCTCCGCACG GACACTGGAG ACACAGTCCC TGACAGGAAG 
AACTTCGATT CCTTACTGTG GGCCATCGTC ACCGTGTTTC AGATCCTCAC TCAGGAGGAC 
TGGAACGTTG TCCTGTACAA TGGCATGGCC TCCACCACCC CCTGGGCCTC CCTCTATTTT 
GTTGCCCTCA TGACCTTTGG CAACTATGTT CTCTTCAATC TCCTGGTGGC TATCCTGGTC 
GAGGGCTTCC AGGCTGAGGG TGATGCTAAT CGCTCCTACT CAGACGAGGA CCAGAGCTCA 
TCCAACTTGG AGGAGCTTGA TAAGCTCCCA GAGGGCCTGG ACAGCAGCAG AGATCTCAAG 
CTCTGCCCAA TACCCATGAC ACCCAATGGA CACCTGGACC CTAGTCTCCC TCTGGGTGGA 
CATCTGGGTC CTGCTGGTGC CATGGGTGCT GCCCCCCGCC TCTCACTGCA GCCAGACCCA 
GTGCTGGTGG CCCTAGAATC CCGGAAAAGC AGTGTCATGT CACTGGGCAG GATGAGCTAT 
GATCAGCGAT CTTTGTCCAG CTCCCGGAGC TCCTACTATG GGCCCTGGGG CCGCAGCGGG 
ACCTGGGCCA GCCGCCGCTC CAGCTGGAAC AGCCTGAAAC ACAAGCCACC CTCAGCTGAG 
CACGAGTCCC TGCTCTCCGG GGAGCGTGGT GGTAGTTGCG TCAGGGCCTG TGAAGGCGCC 
CGGGAGGATG CGCCACCTCG CGCTGCACCC CTGCATGCCC CGCACACCCA CCACGCGCAT 
CATGGACCCC ACTTGGCACA CCGTCACCGC CACCACCGTC GGACACTGTC CCTTGATACC 
AGGGACTCTG TTGACCTGGC AGAGCTGGTG CCCGTGGTGG GTGCCCACTC GCGGGCCGCT 
TGGAGGGCGG CAGGCCAGGC CCCTGGGCAC GAGGACTGCA ATGGCAGAAT GCCCAACATA 
GCCAAGGATG TCTTCACCAA GATGGACGAC CGCCGCGACC GTGGGGAGGA CGAGGAGGAG 
ATCGACTACA CCCTGTGTTT CCGCGTCCGC AAGATGATTG ATGTGTACAA GCCTGACTGG 
TGCGAAGTCC GCGAGGACTG GTCGGTCTAC CTCTTCTCCC CTGAGAACAA GTTCCGGATC 
CTGTGTCAGA CCATTATTGC TCACAAGCTT TTTGACTACG TGGTCTTGGC CTTCATCTTC 
CTCAACTGTA TCACCATTGC TCTGGAGAGG CCCCAGATCG AAGCTGGTAG CACTGAGCGC 
ATCTTCCTCA CGGTGTCTAA CTACATCTTC ACAGCCATCT TTGTGGGCGA GATGACACTG 
AAGGTGGTCT CTTTGGGCCT ATACTTCGGT GAGCAAGCAT ACTTGCGTAG CAGCTGGAAC 
GTCCTGGATG GTTTCCTGGT CTTTGTGTCT ATCATCGATA TCGTGGTATC CGTGGCCTCT 
GCAGGGGGAG CCAAGATCCT GGGGGTCCTC CGGGTCCTGC GGCTCCTGCG TACCTTACGT 
CCTTTGAGGG TCATCAGCCG GGCCCCTGGG CTGAAGCTGG TGGTAGAGAC GCTCATCTCC 
TCCCTCAAGC CCATTGGAAA CATTGTCCTC ATCTGCTGCG CCTTCTTCAT CATCTTCGGT 
ATCCTGGGGG TGCAGCTTTT CAAAGGCAAG TTCTATCACT GTCTGGGAGT GGACACCCGA 
AACATCACCA ACCGATCAGA CTGTGTGGCC GCCAACTACC GCTGGGTGCA TCACAAATAC 
AACTTTGATA ACCTGGGCCA GGCTTTGATG TCCCTCTTTG TCTTGGCCTC CAAGGATGGC 
TGGGTGAACA TCATGTATAA TGGATTGGAT GCTGTTGCTG TGGACCAGCA GCCGGTGACA 
AACCACAACC CCTGGATGCT GCTGTACTTC ATCTCCTTCC TGCTCATCGT CAGCTTCTTC 
GTGCTCAACA TGTTCGTGGG AGTGGTCGTG GAGAACTTCC ACAAGTGCCG GCAGCATCAG 
GAGGCTGAGG AGGCACGGCG GCGTGAGGAG AAACGGCTAC GGCGCCTGGA AAAGAAGCGC 
CGTAAGGCTC AGAGGCTGCC CTACTATGCC ACCTACTGTC CCACAAGACT GCTCATCCAC 
TCCATGTGCA CTAGCCACTA CCTGGACATC TTCATCACCT TCATCATCTG CCTCAACGTG 
GTCACCATGT CCCTGGAGCA CTACAACCAG CCTACATCCC TAGAGACAGC CCTCAAGTAC 
TGCAACTACA TGTTCACCAC TGTCTTTGTG CTGGAGGCTG TGCTGAAGCT GGTGGCGTTT 
GGCCTGAGGC GTTTCTTCAA GGACCGATGG AACCAGCTGG ACTTGGCCAT TGTGCTGCTG 
TCTGTCATGG GCATCACACT GGAGGAGATT GAGATCAATG CGGCCCTGCC CATCAACCCC 
ACCATCATCC GTATCATGCG TGTTCTGCGT ATTGCCCGGG TGTTGAAGCT GTTGAAGATG 
GCCACAGGAA TGCGGGCCCT GCTGGACACA GTGGTACAGG CTCTGCCCCA GGTGGGCAAC 
CTGGGCCTCC TCTTCATGCT GCTCTTCTTC ATCTACGCCG CTCTGGGAGT GGAGCTCTTC 
GGAAAGCTGG TCTGCAATGA CGAGAACCCG TGTGAGGGCA TGAGCCGGCA CGCCACCTTC 
GAGAACTTCG GCATGGCCTT CCTCACACTC TTCCAGGTCT CCACAGGCGA TAACTGGAAT 
GGGATTATGA AGGACACTCT GCGAGACTGC ACCCACGACG AGCGCAGCTG CCTGAGCAGC 
CTGCAGTTTG TGTCGCCTCT CTACTTTGTG AGCTTCGTAC TCACAGCGCA GTTTGTGCTC 
ATCAACGTGG TGGTGGCCGT GCTGATGAAA CATCTGGATG ACAGCAACAA GGAGGCCCAG 
GAGGATGCAG AGATGGATGC TGAGATCGAG CTGGAGATGG CCCATGGCCT AGGCCCCGGG 
CCCGGCCCCT GTCCCTGTCC CTGTCCCTGC CCCTGCCCCT GTCCCTGTCC TGGCCCGAGG 
ATGCCCACCA GTTCACCGGG GGCTCCAGGC CGAGGATCAG GAGGGGCAGG CGTTGGAGGC 
GACACTGAGA GCCACCTGTG CCGGCACTGC TATTCTCCAG CCCAGGACTC CTTGGAGGGG 
GAGCTGACCA TCATTGACAA CCTGTCTGGC TCCATCTTCC ACCACTACTC CTCACCTGCC 
GGCTGTGACA AGTGTCACCA TGACAAGCAA GAGGGACCTG TTGCTCAGCG CTCCAGGCCC 
AGGCTGCCCC ATCCCAGCCC CAGGGTGACC CGAGGCCACC ACCTCCCCCC GCAGGTGCAG 
CTGGCTGAGA CAGAGGCCTT CTCCCTGAAC TCAGACAGGT CCTCGTCCGT CCTGCTGGGG 
GATGACCTGA GTCTTGAGGA CCCCACGGCC TGCCCGCAGG GCCCCAAGGA GAGCAAGGGT 
GAGCTAGAGC CTCCGGAGCC CATGCAGGCT GGAGACCTGG ATGAATGCTT CTTCCCCTTT 
GCCGGTGAGC CAGTGTCTGC AGGCCCAGAG AGCCTGCTGT GCGAGATGGG GGCCATTCCA 
TTCAACCCCG TCCAGTCCTG GCTCAAACAT GAGAGCAACC AAGCACCCCC AAGCCCTTTC 
TCCCCGGATG GTTCCAGCCC TCTGCTGCAG ATGCCTGCTG AGTTCTTCCA CCCTGCTGTG 
TCTGCCAGCC AGAAGGGGCA GGAACCGGGC ATGAGTTCAG GGACCCTGCC CAAGATTGCA 
CTTCAGGGGT CCTGGGCATC CCTGAGGTCA CCGAGTGTCA ACTGCACCCT CTTGCGCCAG 
GCTACTGTGA GTGACACGTC TTTGGATGCC AGCCCTAGCA GCTCAGCGGG CAGCCTGCAG 
ACCACACTGG AGGACAGTCT GACCCTGAGT GACAGTCCTC GGCGTGCCCT GGGACCACCA 
GTCCAGGTGC CAGGGCCACG GGCTAGCCTG TCACCTGCCA CCCGGCGCCG CCTCAGCCTG 
CGGGGGCGTG GCCTGTTTAG TCTGCGTGGG CTGCGGGCCC ATCAGCGTAG CCACAGCAGC 
GGTGGCTCCA CCAGCCCTGG CTGCACTTAC CACGACTCCA TGGACCCCTC TGATGAGGAG 
GGCCGCGGGG GCGCAGGTGG TGGGGGCGCG GGCAGTGAGC ACTCCGAGAC CCTCAGCAGC 
CTGTCACTCA CATCTCTCTT CTGCCTACCA CCCACGCTGC CACCACCCGG TCTCACCCCA 
GCCAGGAAGT TCAGCAGTAC CAGCAGCCTG GCTGCTGGAC CTGGCCGTCC TGGCGCCACT 
GTCTCGGTCC GCGGCCTGGC CAGGAGTCCC TCATGGGCTG CAGACCGCAG CAAGGACCCA 
CCAGGCCAGG CACAGCTGGC CTCCGGCTTC GGCTCCTCAG CACCCGAGCC ACAGCCACCT 
CCCGGGGAGT CAACGGATGC GGCGAGCAAA AGGAAGAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1i ( XM_030248558.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030104418.1 Mus musculus calcium channel, voltage-dependent, alpha 1I subunit (Cacna1i), transcript variant X3, mRNA.

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CloneID	OMu86721
Clone ID Related Accession (Same CDS sequence)	XM_030248558.1
Accession Version	XM_030248558.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6561bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1I isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGACA GCAACTTACC ACCCTCATCT TCAGCAGCCC CGGACCCTGA GCCGGGAATC 
ACTGAGCAGC CGGGGCCCCG GAGTCCCCCT CCATCCCCTC CAGGCCTGGA GGAGCCATTG 
GATGGAACCA ACCCTGACGT CCCGCATCCA GACCTGGCAC CCGTTGCCTT CTTCTGCCTG 
CGCCAGACCA CGAGCCCACG GAACTGGTGC ATCAAGATGG TATGTAACCC GTGGTTCGAG 
TGTGTGAGCA TGCTGGTCAT CCTGCTGAAC TGTGTGACCC TGGGCATGTA CCAGCCATGT 
GACGATATGG AGTGCCTCTC AGACCGCTGC AAGATCCTGC AGGTCTTTGA TGACTTCATC 
TTCATCTTCT TCGCCATGGA GATGGTGCTT AAGATGGTGG CCCTGGGCAT TTTTGGCAAG 
AAGTGCTACC TCGGGGACAC ATGGAACCGC TTGGACTTCT TCATTGTCAT GGCAGGGATG 
GTCGAGTACT CTCTGGACCT ACAGAACATC AACCTGTCAG CCATCCGCAC TGTGCGTGTC 
CTGAGGCCTC TCAAAGCCAT CAACCGTGTT CCCAGCATGC GGATTCTGGT GAACCTGCTG 
CTGGACACGC TGCCCATGCT GGGAAACGTG CTCCTGCTCT GTTTCTTCGT CTTCTTCATC 
TTTGGCATCA TCGGCGTGCA GCTCTGGGCA GGCCTGCTGC GGAACCGCTG CTTCCTGGAG 
GAGAACTTCA CCATACAAGG GGATGTGGCC CTGCCCCCTT ACTACCAACC AGAAGAGGAC 
GACGAGATGC CCTTCATCTG CTCCCTGTCG GGGGACAATG GCATTATGGG CTGTCACGAG 
ATCCCTCCAC TCAAGGAGCA GGGCCGAGAG TGCTGCCTGT CCAAAGATGA CATGTACGAC 
TTCGGGGCGG GACGCCAAGA CCTCAACGCC AGCGGCCTCT GCGTCAACTG GAACCGCTAC 
TACAACGTCT GCCGCACGGG CAACGCCAAC CCTCACAAGG GCGCCATCAA CTTTGACAAC 
ATTGGCTATG CCTGGATCGT GATCTTTCAG GTGATCACTC TGGAAGGCTG GGTGGAGATC 
ATGTACTACG TGATGGATGC ACATTCTTTC TACAACTTCA TCTACTTCAT CCTGCTCATC 
ATAGTGGGCT CCTTCTTCAT GATCAACCTG TGCCTCGTTG TCATAGCGAC CCAGTTCTCT 
GAGACCAAGC AACGGGAGCA CCGGCTGATG CTGGAGCAAC GCCAGCGCTA CCTGTCCTCC 
AGCACGGTGG CCAGTTACGC CGAGCCCGGC GACTGCTATG AGGAGATCTT CCAATATGTC 
TGTCACATCC TTCGCAAGGC CAAGCGCCGT GCTCTAGGCC TCTACCAGGC CCTGCAGAAC 
CGGCGCCAGG CCACGGGCCC CGGGACACCA GCCCCTGCCA AGCCTGGGCC CCATGCCAAG 
GAGCCCAGCC ACTGCAAGCT GTGCCCACGA CACAGTCCCC TGGACACCAC TCCCCACACA 
CTGGTGCAGC CCATCTCTGC CATTCTGGCC TCCGATCCCA GCAGCTGCCC TCGCTGCCAG 
CACGAGGCAG GCAGGCGGCC CTCTGGCCTG GGCAGCACTG ACTCGGGCCA GGAAGGCTCA 
GGTTCTGGCG GTTCTGCAGA GGCTGAAGCC AATGGCGATG GACCCCAAAG CAGTGAGGAT 
GGGGTCTCCT CAGGTTTGGG GAAGGAGGAG GAACAGGAGG ACGGGGCAGC CCGACTGTGT 
GGGGATGTGT GGCGAGAGAC ACGGGCCAAG CTGCGGGGCA TTGTGGACAG CAAGTACTTC 
AACAGAGGCA TCATGATGGC TATTCTGGTT AACACTGTCA GCATGGGCAT CGAGCACCAT 
GAACAGCCAG AGGAACTGAC CAACATCCTG GAGATCTGCA ACGTGGTCTT CACCAGCATG 
TTTGCCCTGG AGATGATTCT GAAGCTGGCT GCCTTTGGGC TCTTTGACTA CCTGCGGAAC 
CCTTACAACA TCTTCGACAG CATCATTGTC ATCATCAGCA TCTGGGAAAT CGTGGGGCAG 
GCTGACGGCG GCCTGTCCGT GCTGCGCACC TTCCGGCTGC TGCGGGTGCT GAAGCTGGTG 
CGCTTCATGC CGGCGCTGCG GCGCCAGCTC GTGGTGCTCA TGAAGACCAT GGACAACGTG 
GCCACCTTCT GCATGCTGCT CATGCTCTTC ATTTTCATCT TCAGCATCCT TGGGATGCAT 
ATCTTTGGCT GCAAATTCAG CCTCCGCACG GACACTGGAG ACACAGTCCC TGACAGGAAG 
AACTTCGATT CCTTACTGTG GGCCATCGTC ACCGTGTTTC AGATCCTCAC TCAGGAGGAC 
TGGAACGTTG TCCTGTACAA TGGCATGGCC TCCACCACCC CCTGGGCCTC CCTCTATTTT 
GTTGCCCTCA TGACCTTTGG CAACTATGTT CTCTTCAATC TCCTGGTGGC TATCCTGGTC 
GAGGGCTTCC AGGCTGAGGG TGATGCTAAT CGCTCCTACT CAGACGAGGA CCAGAGCTCA 
TCCAACTTGG AGGAGCTTGA TAAGCTCCCA GAGGGCCTGG ACAGCAGCAG AGATCTCAAG 
CTCTGCCCAA TACCCATGAC ACCCAATGGA CACCTGGACC CTAGTCTCCC TCTGGGTGGA 
CATCTGGGTC CTGCTGGTGC CATGGGTGCT GCCCCCCGCC TCTCACTGCA GCCAGACCCA 
GTGCTGGTGG CCCTAGAATC CCGGAAAAGC AGTGTCATGT CACTGGGCAG GATGAGCTAT 
GATCAGCGAT CTTTGTCCAG CTCCCGGAGC TCCTACTATG GGCCCTGGGG CCGCAGCGGG 
ACCTGGGCCA GCCGCCGCTC CAGCTGGAAC AGCCTGAAAC ACAAGCCACC CTCAGCTGAG 
CACGAGTCCC TGCTCTCCGG GGAGCGTGGT GGTAGTTGCG TCAGGGCCTG TGAAGGCGCC 
CGGGAGGATG CGCCACCTCG CGCTGCACCC CTGCATGCCC CGCACACCCA CCACGCGCAT 
CATGGACCCC ACTTGGCACA CCGTCACCGC CACCACCGTC GGACACTGTC CCTTGATACC 
AGGGACTCTG TTGACCTGGC AGAGCTGGTG CCCGTGGTGG GTGCCCACTC GCGGGCCGCT 
TGGAGGGCGG CAGGCCAGGC CCCTGGGCAC GAGGACTGCA ATGGCAGAAT GCCCAACATA 
GCCAAGGATG TCTTCACCAA GATGGACGAC CGCCGCGACC GTGGGGAGGA CGAGGAGGAG 
ATCGACTACA CCCTGTGTTT CCGCGTCCGC AAGATGATTG ATGTGTACAA GCCTGACTGG 
TGCGAAGTCC GCGAGGACTG GTCGGTCTAC CTCTTCTCCC CTGAGAACAA GTTCCGGATC 
CTGTGTCAGA CCATTATTGC TCACAAGCTT TTTGACTACG TGGTCTTGGC CTTCATCTTC 
CTCAACTGTA TCACCATTGC TCTGGAGAGG CCCCAGATCG AAGCTGGTAG CACTGAGCGC 
ATCTTCCTCA CGGTGTCTAA CTACATCTTC ACAGCCATCT TTGTGGGCGA GATGACACTG 
AAGGTGGTCT CTTTGGGCCT ATACTTCGGT GAGCAAGCAT ACTTGCGTAG CAGCTGGAAC 
GTCCTGGATG GTTTCCTGGT CTTTGTGTCT ATCATCGATA TCGTGGTATC CGTGGCCTCT 
GCAGGGGGAG CCAAGATCCT GGGGGTCCTC CGGGTCCTGC GGCTCCTGCG TACCTTACGT 
CCTTTGAGGG TCATCAGCCG GGCCCCTGGG CTGAAGCTGG TGGTAGAGAC GCTCATCTCC 
TCCCTCAAGC CCATTGGAAA CATTGTCCTC ATCTGCTGCG CCTTCTTCAT CATCTTCGGT 
ATCCTGGGGG TGCAGCTTTT CAAAGGCAAG TTCTATCACT GTCTGGGAGT GGACACCCGA 
AACATCACCA ACCGATCAGA CTGTGTGGCC GCCAACTACC GCTGGGTGCA TCACAAATAC 
AACTTTGATA ACCTGGGCCA GGCTTTGATG TCCCTCTTTG TCTTGGCCTC CAAGGATGGC 
TGGGTGAACA TCATGTATAA TGGATTGGAT GCTGTTGCTG TGGACCAGCA GCCGGTGACA 
AACCACAACC CCTGGATGCT GCTGTACTTC ATCTCCTTCC TGCTCATCGT CAGCTTCTTC 
GTGCTCAACA TGTTCGTGGG AGTGGTCGTG GAGAACTTCC ACAAGTGCCG GCAGCATCAG 
GAGGCTGAGG AGGCACGGCG GCGTGAGGAG AAACGGCTAC GGCGCCTGGA AAAGAAGCGC 
CGTAAGGCTC AGAGGCTGCC CTACTATGCC ACCTACTGTC CCACAAGACT GCTCATCCAC 
TCCATGTGCA CTAGCCACTA CCTGGACATC TTCATCACCT TCATCATCTG CCTCAACGTG 
GTCACCATGT CCCTGGAGCA CTACAACCAG CCTACATCCC TAGAGACAGC CCTCAAGTAC 
TGCAACTACA TGTTCACCAC TGTCTTTGTG CTGGAGGCTG TGCTGAAGCT GGTGGCGTTT 
GGCCTGAGGC GTTTCTTCAA GGACCGATGG AACCAGCTGG ACTTGGCCAT TGTGCTGCTG 
TCTGTCATGG GCATCACACT GGAGGAGATT GAGATCAATG CGGCCCTGCC CATCAACCCC 
ACCATCATCC GTATCATGCG TGTTCTGCGT ATTGCCCGGG TGTTGAAGCT GTTGAAGATG 
GCCACAGGAA TGCGGGCCCT GCTGGACACA GTGGTACAGG CTCTGCCCCA GGTGGGCAAC 
CTGGGCCTCC TCTTCATGCT GCTCTTCTTC ATCTACGCCG CTCTGGGAGT GGAGCTCTTC 
GGAAAGCTGG TCTGCAATGA CGAGAACCCG TGTGAGGGCA TGAGCCGGCA CGCCACCTTC 
GAGAACTTCG GCATGGCCTT CCTCACACTC TTCCAGGTCT CCACAGGCGA TAACTGGAAT 
GGGATTATGA AGGACACTCT GCGAGACTGC ACCCACGACG AGCGCAGCTG CCTGAGCAGC 
CTGCAGTTTG TGTCGCCTCT CTACTTTGTG AGCTTCGTAC TCACAGCGCA GTTTGTGCTC 
ATCAACGTGG TGGTGGCCGT GCTGATGAAA CATCTGGATG ACAGCAACAA GGAGGCCCAG 
GAGGATGCAG AGATGGATGC TGAGATCGAG CTGGAGATGG CCCATGGCCT AGGCCCCGGG 
CCCGGCCCCT GTCCCTGTCC CTGTCCCTGC CCCTGCCCCT GTCCCTGTCC TGGCCCGAGG 
ATGCCCACCA GTTCACCGGG GGCTCCAGGC CGAGGATCAG GAGGGGCAGG CGTTGGAGGC 
GACACTGAGA GCCACCTGTG CCGGCACTGC TATTCTCCAG CCCAGGACTC CTTGGAGGGG 
GAGCTGACCA TCATTGACAA CCTGTCTGGC TCCATCTTCC ACCACTACTC CTCACCTGCC 
GGCTGTGACA AGTGTCACCA TGACAAGCAA GAGGTGCAGC TGGCTGAGAC AGAGGCCTTC 
TCCCTGAACT CAGACAGGTC CTCGTCCGTC CTGCTGGGGG ATGACCTGAG TCTTGAGGAC 
CCCACGGCCT GCCCGCAGGG CCCCAAGGAG AGCAAGGGTG AGCTAGAGCC TCCGGAGCCC 
ATGCAGGCTG GAGACCTGGA TGAATGCTTC TTCCCCTTTG CCGGTGAGCC AGTGTCTGCA 
GGCCCAGAGA GCCTGCTGTG CGAGATGGGG GCCATTCCAT TCAACCCCGT CCAGTCCTGG 
CTCAAACATG AGAGCAACCA AGCACCCCCA AGCCCTTTCT CCCCGGATGG TTCCAGCCCT 
CTGCTGCAGA TGCCTGCTGA GTTCTTCCAC CCTGCTGTGT CTGCCAGCCA GAAGGGGCAG 
GAACCGGGCA TGAGTTCAGG GACCCTGCCC AAGATTGCAC TTCAGGGGTC CTGGGCATCC 
CTGAGGTCAC CGAGTGTCAA CTGCACCCTC TTGCGCCAGG CTACTGTGAG TGACACGTCT 
TTGGATGCCA GCCCTAGCAG CTCAGCGGGC AGCCTGCAGA CCACACTGGA GGACAGTCTG 
ACCCTGAGTG ACAGTCCTCG GCGTGCCCTG GGACCACCAG TCCAGGTGCC AGGGCCACGG 
GCTAGCCTGT CACCTGCCAC CCGGCGCCGC CTCAGCCTGC GGGGGCGTGG CCTGTTTAGT 
CTGCGTGGGC TGCGGGCCCA TCAGCGTAGC CACAGCAGCG GTGGCTCCAC CAGCCCTGGC 
TGCACTTACC ACGACTCCAT GGACCCCTCT GATGAGGAGG GCCGCGGGGG CGCAGGTGGT 
GGGGGCGCGG GCAGTGAGCA CTCCGAGACC CTCAGCAGCC TGTCACTCAC ATCTCTCTTC 
TGCCTACCAC CCACGCTGCC ACCACCCGGT CTCACCCCAG CCAGGAAGTT CAGCAGTACC 
AGCAGCCTGG CTGCTGGACC TGGCCGTCCT GGCGCCACTG TCTCGGTCCG CGGCCTGGCC 
AGGAGTCCCT CATGGGCTGC AGACCGCAGC AAGGACCCAC CAGGCCAGGC ACAGCTGGCC 
TCCGGCTTCG GCTCCTCAGC ACCCGAGCCA CAGCCACCTC CCGGGGAGTC AACGGATGCG 
GCGAGCAAAA GGAAGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030104418.1
CDS	557..7117
Translation	MADSNLPPSSSAAPDPEPGITEQPGPRSPPPSPPGLEEPLDGTNPDVPHPDLAPVAFFCLRQTTSPRNWCIKMVCNPWFECVSMLVILLNCVTLGMYQPCDDMECLSDRCKILQVFDDFIFIFFAMEMVLKMVALGIFGKKCYLGDTWNRLDFFIVMAGMVEYSLDLQNINLSAIRTVRVLRPLKAINRVPSMRILVNLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLWAGLLRNRCFLEENFTIQGDVALPPYYQPEEDDEMPFICSLSGDNGIMGCHEIPPLKEQGRECCLSKDDMYDFGAGRQDLNASGLCVNWNRYYNVCRTGNANPHKGAINFDNIGYAWIVIFQVITLEGWVEIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQREHRLMLEQRQRYLSSSTVASYAEPGDCYEEIFQYVCHILRKAKRRALGLYQALQNRRQATGPGTPAPAKPGPHAKEPSHCKLCPRHSPLDTTPHTLVQPISAILASDPSSCPRCQHEAGRRPSGLGSTDSGQEGSGSGGSAEAEANGDGPQSSEDGVSSGLGKEEEQEDGAARLCGDVWRETRAKLRGIVDSKYFNRGIMMAILVNTVSMGIEHHEQPEELTNILEICNVVFTSMFALEMILKLAAFGLFDYLRNPYNIFDSIIVIISIWEIVGQADGGLSVLRTFRLLRVLKLVRFMPALRRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHIFGCKFSLRTDTGDTVPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMASTTPWASLYFVALMTFGNYVLFNLLVAILVEGFQAEGDANRSYSDEDQSSSNLEELDKLPEGLDSSRDLKLCPIPMTPNGHLDPSLPLGGHLGPAGAMGAAPRLSLQPDPVLVALESRKSSVMSLGRMSYDQRSLSSSRSSYYGPWGRSGTWASRRSSWNSLKHKPPSAEHESLLSGERGGSCVRACEGAREDAPPRAAPLHAPHTHHAHHGPHLAHRHRHHRRTLSLDTRDSVDLAELVPVVGAHSRAAWRAAGQAPGHEDCNGRMPNIAKDVFTKMDDRRDRGEDEEEIDYTLCFRVRKMIDVYKPDWCEVREDWSVYLFSPENKFRILCQTIIAHKLFDYVVLAFIFLNCITIALERPQIEAGSTERIFLTVSNYIFTAIFVGEMTLKVVSLGLYFGEQAYLRSSWNVLDGFLVFVSIIDIVVSVASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLKPIGNIVLICCAFFIIFGILGVQLFKGKFYHCLGVDTRNITNRSDCVAANYRWVHHKYNFDNLGQALMSLFVLASKDGWVNIMYNGLDAVAVDQQPVTNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEAEEARRREEKRLRRLEKKRRKAQRLPYYATYCPTRLLIHSMCTSHYLDIFITFIICLNVVTMSLEHYNQPTSLETALKYCNYMFTTVFVLEAVLKLVAFGLRRFFKDRWNQLDLAIVLLSVMGITLEEIEINAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGKLVCNDENPCEGMSRHATFENFGMAFLTLFQVSTGDNWNGIMKDTLRDCTHDERSCLSSLQFVSPLYFVSFVLTAQFVLINVVVAVLMKHLDDSNKEAQEDAEMDAEIELEMAHGLGPGPGPCPCPCPCPCPCPCPGPRMPTSSPGAPGRGSGGAGVGGDTESHLCRHCYSPAQDSLEGELTIIDNLSGSIFHHYSSPAGCDKCHHDKQEVQLAETEAFSLNSDRSSSVLLGDDLSLEDPTACPQGPKESKGELEPPEPMQAGDLDECFFPFAGEPVSAGPESLLCEMGAIPFNPVQSWLKHESNQAPPSPFSPDGSSPLLQMPAEFFHPAVSASQKGQEPGMSSGTLPKIALQGSWASLRSPSVNCTLLRQATVSDTSLDASPSSSAGSLQTTLEDSLTLSDSPRRALGPPVQVPGPRASLSPATRRRLSLRGRGLFSLRGLRAHQRSHSSGGSTSPGCTYHDSMDPSDEEGRGGAGGGGAGSEHSETLSSLSLTSLFCLPPTLPPPGLTPARKFSSTSSLAAGPGRPGATVSVRGLARSPSWAADRSKDPPGQAQLASGFGSSAPEPQPPPGESTDAASKRKR

Target ORF information:

RefSeq Version	XM_030248558.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, alpha 1I subunit (Cacna1i), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030248558.1

ORF Insert Sequence:

ATGGCTGACA GCAACTTACC ACCCTCATCT TCAGCAGCCC CGGACCCTGA GCCGGGAATC 
ACTGAGCAGC CGGGGCCCCG GAGTCCCCCT CCATCCCCTC CAGGCCTGGA GGAGCCATTG 
GATGGAACCA ACCCTGACGT CCCGCATCCA GACCTGGCAC CCGTTGCCTT CTTCTGCCTG 
CGCCAGACCA CGAGCCCACG GAACTGGTGC ATCAAGATGG TATGTAACCC GTGGTTCGAG 
TGTGTGAGCA TGCTGGTCAT CCTGCTGAAC TGTGTGACCC TGGGCATGTA CCAGCCATGT 
GACGATATGG AGTGCCTCTC AGACCGCTGC AAGATCCTGC AGGTCTTTGA TGACTTCATC 
TTCATCTTCT TCGCCATGGA GATGGTGCTT AAGATGGTGG CCCTGGGCAT TTTTGGCAAG 
AAGTGCTACC TCGGGGACAC ATGGAACCGC TTGGACTTCT TCATTGTCAT GGCAGGGATG 
GTCGAGTACT CTCTGGACCT ACAGAACATC AACCTGTCAG CCATCCGCAC TGTGCGTGTC 
CTGAGGCCTC TCAAAGCCAT CAACCGTGTT CCCAGCATGC GGATTCTGGT GAACCTGCTG 
CTGGACACGC TGCCCATGCT GGGAAACGTG CTCCTGCTCT GTTTCTTCGT CTTCTTCATC 
TTTGGCATCA TCGGCGTGCA GCTCTGGGCA GGCCTGCTGC GGAACCGCTG CTTCCTGGAG 
GAGAACTTCA CCATACAAGG GGATGTGGCC CTGCCCCCTT ACTACCAACC AGAAGAGGAC 
GACGAGATGC CCTTCATCTG CTCCCTGTCG GGGGACAATG GCATTATGGG CTGTCACGAG 
ATCCCTCCAC TCAAGGAGCA GGGCCGAGAG TGCTGCCTGT CCAAAGATGA CATGTACGAC 
TTCGGGGCGG GACGCCAAGA CCTCAACGCC AGCGGCCTCT GCGTCAACTG GAACCGCTAC 
TACAACGTCT GCCGCACGGG CAACGCCAAC CCTCACAAGG GCGCCATCAA CTTTGACAAC 
ATTGGCTATG CCTGGATCGT GATCTTTCAG GTGATCACTC TGGAAGGCTG GGTGGAGATC 
ATGTACTACG TGATGGATGC ACATTCTTTC TACAACTTCA TCTACTTCAT CCTGCTCATC 
ATAGTGGGCT CCTTCTTCAT GATCAACCTG TGCCTCGTTG TCATAGCGAC CCAGTTCTCT 
GAGACCAAGC AACGGGAGCA CCGGCTGATG CTGGAGCAAC GCCAGCGCTA CCTGTCCTCC 
AGCACGGTGG CCAGTTACGC CGAGCCCGGC GACTGCTATG AGGAGATCTT CCAATATGTC 
TGTCACATCC TTCGCAAGGC CAAGCGCCGT GCTCTAGGCC TCTACCAGGC CCTGCAGAAC 
CGGCGCCAGG CCACGGGCCC CGGGACACCA GCCCCTGCCA AGCCTGGGCC CCATGCCAAG 
GAGCCCAGCC ACTGCAAGCT GTGCCCACGA CACAGTCCCC TGGACACCAC TCCCCACACA 
CTGGTGCAGC CCATCTCTGC CATTCTGGCC TCCGATCCCA GCAGCTGCCC TCGCTGCCAG 
CACGAGGCAG GCAGGCGGCC CTCTGGCCTG GGCAGCACTG ACTCGGGCCA GGAAGGCTCA 
GGTTCTGGCG GTTCTGCAGA GGCTGAAGCC AATGGCGATG GACCCCAAAG CAGTGAGGAT 
GGGGTCTCCT CAGGTTTGGG GAAGGAGGAG GAACAGGAGG ACGGGGCAGC CCGACTGTGT 
GGGGATGTGT GGCGAGAGAC ACGGGCCAAG CTGCGGGGCA TTGTGGACAG CAAGTACTTC 
AACAGAGGCA TCATGATGGC TATTCTGGTT AACACTGTCA GCATGGGCAT CGAGCACCAT 
GAACAGCCAG AGGAACTGAC CAACATCCTG GAGATCTGCA ACGTGGTCTT CACCAGCATG 
TTTGCCCTGG AGATGATTCT GAAGCTGGCT GCCTTTGGGC TCTTTGACTA CCTGCGGAAC 
CCTTACAACA TCTTCGACAG CATCATTGTC ATCATCAGCA TCTGGGAAAT CGTGGGGCAG 
GCTGACGGCG GCCTGTCCGT GCTGCGCACC TTCCGGCTGC TGCGGGTGCT GAAGCTGGTG 
CGCTTCATGC CGGCGCTGCG GCGCCAGCTC GTGGTGCTCA TGAAGACCAT GGACAACGTG 
GCCACCTTCT GCATGCTGCT CATGCTCTTC ATTTTCATCT TCAGCATCCT TGGGATGCAT 
ATCTTTGGCT GCAAATTCAG CCTCCGCACG GACACTGGAG ACACAGTCCC TGACAGGAAG 
AACTTCGATT CCTTACTGTG GGCCATCGTC ACCGTGTTTC AGATCCTCAC TCAGGAGGAC 
TGGAACGTTG TCCTGTACAA TGGCATGGCC TCCACCACCC CCTGGGCCTC CCTCTATTTT 
GTTGCCCTCA TGACCTTTGG CAACTATGTT CTCTTCAATC TCCTGGTGGC TATCCTGGTC 
GAGGGCTTCC AGGCTGAGGG TGATGCTAAT CGCTCCTACT CAGACGAGGA CCAGAGCTCA 
TCCAACTTGG AGGAGCTTGA TAAGCTCCCA GAGGGCCTGG ACAGCAGCAG AGATCTCAAG 
CTCTGCCCAA TACCCATGAC ACCCAATGGA CACCTGGACC CTAGTCTCCC TCTGGGTGGA 
CATCTGGGTC CTGCTGGTGC CATGGGTGCT GCCCCCCGCC TCTCACTGCA GCCAGACCCA 
GTGCTGGTGG CCCTAGAATC CCGGAAAAGC AGTGTCATGT CACTGGGCAG GATGAGCTAT 
GATCAGCGAT CTTTGTCCAG CTCCCGGAGC TCCTACTATG GGCCCTGGGG CCGCAGCGGG 
ACCTGGGCCA GCCGCCGCTC CAGCTGGAAC AGCCTGAAAC ACAAGCCACC CTCAGCTGAG 
CACGAGTCCC TGCTCTCCGG GGAGCGTGGT GGTAGTTGCG TCAGGGCCTG TGAAGGCGCC 
CGGGAGGATG CGCCACCTCG CGCTGCACCC CTGCATGCCC CGCACACCCA CCACGCGCAT 
CATGGACCCC ACTTGGCACA CCGTCACCGC CACCACCGTC GGACACTGTC CCTTGATACC 
AGGGACTCTG TTGACCTGGC AGAGCTGGTG CCCGTGGTGG GTGCCCACTC GCGGGCCGCT 
TGGAGGGCGG CAGGCCAGGC CCCTGGGCAC GAGGACTGCA ATGGCAGAAT GCCCAACATA 
GCCAAGGATG TCTTCACCAA GATGGACGAC CGCCGCGACC GTGGGGAGGA CGAGGAGGAG 
ATCGACTACA CCCTGTGTTT CCGCGTCCGC AAGATGATTG ATGTGTACAA GCCTGACTGG 
TGCGAAGTCC GCGAGGACTG GTCGGTCTAC CTCTTCTCCC CTGAGAACAA GTTCCGGATC 
CTGTGTCAGA CCATTATTGC TCACAAGCTT TTTGACTACG TGGTCTTGGC CTTCATCTTC 
CTCAACTGTA TCACCATTGC TCTGGAGAGG CCCCAGATCG AAGCTGGTAG CACTGAGCGC 
ATCTTCCTCA CGGTGTCTAA CTACATCTTC ACAGCCATCT TTGTGGGCGA GATGACACTG 
AAGGTGGTCT CTTTGGGCCT ATACTTCGGT GAGCAAGCAT ACTTGCGTAG CAGCTGGAAC 
GTCCTGGATG GTTTCCTGGT CTTTGTGTCT ATCATCGATA TCGTGGTATC CGTGGCCTCT 
GCAGGGGGAG CCAAGATCCT GGGGGTCCTC CGGGTCCTGC GGCTCCTGCG TACCTTACGT 
CCTTTGAGGG TCATCAGCCG GGCCCCTGGG CTGAAGCTGG TGGTAGAGAC GCTCATCTCC 
TCCCTCAAGC CCATTGGAAA CATTGTCCTC ATCTGCTGCG CCTTCTTCAT CATCTTCGGT 
ATCCTGGGGG TGCAGCTTTT CAAAGGCAAG TTCTATCACT GTCTGGGAGT GGACACCCGA 
AACATCACCA ACCGATCAGA CTGTGTGGCC GCCAACTACC GCTGGGTGCA TCACAAATAC 
AACTTTGATA ACCTGGGCCA GGCTTTGATG TCCCTCTTTG TCTTGGCCTC CAAGGATGGC 
TGGGTGAACA TCATGTATAA TGGATTGGAT GCTGTTGCTG TGGACCAGCA GCCGGTGACA 
AACCACAACC CCTGGATGCT GCTGTACTTC ATCTCCTTCC TGCTCATCGT CAGCTTCTTC 
GTGCTCAACA TGTTCGTGGG AGTGGTCGTG GAGAACTTCC ACAAGTGCCG GCAGCATCAG 
GAGGCTGAGG AGGCACGGCG GCGTGAGGAG AAACGGCTAC GGCGCCTGGA AAAGAAGCGC 
CGTAAGGCTC AGAGGCTGCC CTACTATGCC ACCTACTGTC CCACAAGACT GCTCATCCAC 
TCCATGTGCA CTAGCCACTA CCTGGACATC TTCATCACCT TCATCATCTG CCTCAACGTG 
GTCACCATGT CCCTGGAGCA CTACAACCAG CCTACATCCC TAGAGACAGC CCTCAAGTAC 
TGCAACTACA TGTTCACCAC TGTCTTTGTG CTGGAGGCTG TGCTGAAGCT GGTGGCGTTT 
GGCCTGAGGC GTTTCTTCAA GGACCGATGG AACCAGCTGG ACTTGGCCAT TGTGCTGCTG 
TCTGTCATGG GCATCACACT GGAGGAGATT GAGATCAATG CGGCCCTGCC CATCAACCCC 
ACCATCATCC GTATCATGCG TGTTCTGCGT ATTGCCCGGG TGTTGAAGCT GTTGAAGATG 
GCCACAGGAA TGCGGGCCCT GCTGGACACA GTGGTACAGG CTCTGCCCCA GGTGGGCAAC 
CTGGGCCTCC TCTTCATGCT GCTCTTCTTC ATCTACGCCG CTCTGGGAGT GGAGCTCTTC 
GGAAAGCTGG TCTGCAATGA CGAGAACCCG TGTGAGGGCA TGAGCCGGCA CGCCACCTTC 
GAGAACTTCG GCATGGCCTT CCTCACACTC TTCCAGGTCT CCACAGGCGA TAACTGGAAT 
GGGATTATGA AGGACACTCT GCGAGACTGC ACCCACGACG AGCGCAGCTG CCTGAGCAGC 
CTGCAGTTTG TGTCGCCTCT CTACTTTGTG AGCTTCGTAC TCACAGCGCA GTTTGTGCTC 
ATCAACGTGG TGGTGGCCGT GCTGATGAAA CATCTGGATG ACAGCAACAA GGAGGCCCAG 
GAGGATGCAG AGATGGATGC TGAGATCGAG CTGGAGATGG CCCATGGCCT AGGCCCCGGG 
CCCGGCCCCT GTCCCTGTCC CTGTCCCTGC CCCTGCCCCT GTCCCTGTCC TGGCCCGAGG 
ATGCCCACCA GTTCACCGGG GGCTCCAGGC CGAGGATCAG GAGGGGCAGG CGTTGGAGGC 
GACACTGAGA GCCACCTGTG CCGGCACTGC TATTCTCCAG CCCAGGACTC CTTGGAGGGG 
GAGCTGACCA TCATTGACAA CCTGTCTGGC TCCATCTTCC ACCACTACTC CTCACCTGCC 
GGCTGTGACA AGTGTCACCA TGACAAGCAA GAGGTGCAGC TGGCTGAGAC AGAGGCCTTC 
TCCCTGAACT CAGACAGGTC CTCGTCCGTC CTGCTGGGGG ATGACCTGAG TCTTGAGGAC 
CCCACGGCCT GCCCGCAGGG CCCCAAGGAG AGCAAGGGTG AGCTAGAGCC TCCGGAGCCC 
ATGCAGGCTG GAGACCTGGA TGAATGCTTC TTCCCCTTTG CCGGTGAGCC AGTGTCTGCA 
GGCCCAGAGA GCCTGCTGTG CGAGATGGGG GCCATTCCAT TCAACCCCGT CCAGTCCTGG 
CTCAAACATG AGAGCAACCA AGCACCCCCA AGCCCTTTCT CCCCGGATGG TTCCAGCCCT 
CTGCTGCAGA TGCCTGCTGA GTTCTTCCAC CCTGCTGTGT CTGCCAGCCA GAAGGGGCAG 
GAACCGGGCA TGAGTTCAGG GACCCTGCCC AAGATTGCAC TTCAGGGGTC CTGGGCATCC 
CTGAGGTCAC CGAGTGTCAA CTGCACCCTC TTGCGCCAGG CTACTGTGAG TGACACGTCT 
TTGGATGCCA GCCCTAGCAG CTCAGCGGGC AGCCTGCAGA CCACACTGGA GGACAGTCTG 
ACCCTGAGTG ACAGTCCTCG GCGTGCCCTG GGACCACCAG TCCAGGTGCC AGGGCCACGG 
GCTAGCCTGT CACCTGCCAC CCGGCGCCGC CTCAGCCTGC GGGGGCGTGG CCTGTTTAGT 
CTGCGTGGGC TGCGGGCCCA TCAGCGTAGC CACAGCAGCG GTGGCTCCAC CAGCCCTGGC 
TGCACTTACC ACGACTCCAT GGACCCCTCT GATGAGGAGG GCCGCGGGGG CGCAGGTGGT 
GGGGGCGCGG GCAGTGAGCA CTCCGAGACC CTCAGCAGCC TGTCACTCAC ATCTCTCTTC 
TGCCTACCAC CCACGCTGCC ACCACCCGGT CTCACCCCAG CCAGGAAGTT CAGCAGTACC 
AGCAGCCTGG CTGCTGGACC TGGCCGTCCT GGCGCCACTG TCTCGGTCCG CGGCCTGGCC 
AGGAGTCCCT CATGGGCTGC AGACCGCAGC AAGGACCCAC CAGGCCAGGC ACAGCTGGCC 
TCCGGCTTCG GCTCCTCAGC ACCCGAGCCA CAGCCACCTC CCGGGGAGTC AACGGATGCG 
GCGAGCAAAA GGAAGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1i ( NM_001044308.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001037773.2 Mus musculus calcium channel, voltage-dependent, alpha 1I subunit (Cacna1i), mRNA.

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CloneID	OMu21028
Clone ID Related Accession (Same CDS sequence)	NM_001044308.2
Accession Version	NM_001044308.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6600bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-28
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1I
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK147585.1, AC155313.2 and CJ259561.1. On Sep 7, 2007 this sequence version replaced NM_001044308.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript that matched the reference genome was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK147585.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGACA GCAACTTACC ACCCTCATCT TCAGCAGCCC CGGACCCTGA GCCGGGAATC 
ACTGAGCAGC CGGGGCCCCG GAGTCCCCCT CCATCCCCTC CAGGCCTGGA GGAGCCATTG 
GATGGAACCA ACCCTGACGT CCCGCATCCA GACCTGGCAC CCGTTGCCTT CTTCTGCCTG 
CGCCAGACCA CGAGCCCACG GAACTGGTGC ATCAAGATGG TATGTAACCC GTGGTTCGAG 
TGTGTGAGCA TGCTGGTCAT CCTGCTGAAC TGTGTGACCC TGGGCATGTA CCAGCCATGT 
GACGATATGG AGTGCCTCTC AGACCGCTGC AAGATCCTGC AGGTCTTTGA TGACTTCATC 
TTCATCTTCT TCGCCATGGA GATGGTGCTT AAGATGGTGG CCCTGGGCAT TTTTGGCAAG 
AAGTGCTACC TCGGGGACAC ATGGAACCGC TTGGACTTCT TCATTGTCAT GGCAGGGATG 
GTCGAGTACT CTCTGGACCT ACAGAACATC AACCTGTCAG CCATCCGCAC TGTGCGTGTC 
CTGAGGCCTC TCAAAGCCAT CAACCGTGTT CCCAGCATGC GGATTCTGGT GAACCTGCTG 
CTGGACACGC TGCCCATGCT GGGAAACGTG CTCCTGCTCT GTTTCTTCGT CTTCTTCATC 
TTTGGCATCA TCGGCGTGCA GCTCTGGGCA GGCCTGCTGC GGAACCGCTG CTTCCTGGAG 
GAGAACTTCA CCATACAAGG GGATGTGGCC CTGCCCCCTT ACTACCAACC AGAAGAGGAC 
GACGAGATGC CCTTCATCTG CTCCCTGTCG GGGGACAATG GCATTATGGG CTGTCACGAG 
ATCCCTCCAC TCAAGGAGCA GGGCCGAGAG TGCTGCCTGT CCAAAGATGA CATGTACGAC 
TTCGGGGCGG GACGCCAAGA CCTCAACGCC AGCGGCCTCT GCGTCAACTG GAACCGCTAC 
TACAACGTCT GCCGCACGGG CAACGCCAAC CCTCACAAGG GCGCCATCAA CTTTGACAAC 
ATTGGCTATG CCTGGATCGT GATCTTTCAG GTGATCACTC TGGAAGGCTG GGTGGAGATC 
ATGTACTACG TGATGGATGC ACATTCTTTC TACAACTTCA TCTACTTCAT CCTGCTCATC 
ATAGTGGGCT CCTTCTTCAT GATCAACCTG TGCCTCGTTG TCATAGCGAC CCAGTTCTCT 
GAGACCAAGC AACGGGAGCA CCGGCTGATG CTGGAGCAAC GCCAGCGCTA CCTGTCCTCC 
AGCACGGTGG CCAGTTACGC CGAGCCCGGC GACTGCTATG AGGAGATCTT CCAATATGTC 
TGTCACATCC TTCGCAAGGC CAAGCGCCGT GCTCTAGGCC TCTACCAGGC CCTGCAGAAC 
CGGCGCCAGG CCACGGGCCC CGGGACACCA GCCCCTGCCA AGCCTGGGCC CCATGCCAAG 
GAGCCCAGCC ACTGCAAGCT GTGCCCACGA CACAGTCCCC TGGACACCAC TCCCCACACA 
CTGGTGCAGC CCATCTCTGC CATTCTGGCC TCCGATCCCA GCAGCTGCCC TCGCTGCCAG 
CACGAGGCAG GCAGGCGGCC CTCTGGCCTG GGCAGCACTG ACTCGGGCCA GGAAGGCTCA 
GGTTCTGGCG GTTCTGCAGA GGCTGAAGCC AATGGCGATG GACCCCAAAG CAGTGAGGAT 
GGGGTCTCCT CAGGTTTGGG GAAGGAGGAG GAACAGGAGG ACGGGGCAGC CCGACTGTGT 
GGGGATGTGT GGCGAGAGAC ACGGGCCAAG CTGCGGGGCA TTGTGGACAG CAAGTACTTC 
AACAGAGGCA TCATGATGGC TATTCTGGTT AACACTGTCA GCATGGGCAT CGAGCACCAT 
GAACAGCCAG AGGAACTGAC CAACATCCTG GAGATCTGCA ACGTGGTCTT CACCAGCATG 
TTTGCCCTGG AGATGATTCT GAAGCTGGCT GCCTTTGGGC TCTTTGACTA CCTGCGGAAC 
CCTTACAACA TCTTCGACAG CATCATTGTC ATCATCAGCA TCTGGGAAAT CGTGGGGCAG 
GCTGACGGCG GCCTGTCCGT GCTGCGCACC TTCCGGCTGC TGCGGGTGCT GAAGCTGGTG 
CGCTTCATGC CGGCGCTGCG GCGCCAGCTC GTGGTGCTCA TGAAGACCAT GGACAACGTG 
GCCACCTTCT GCATGCTGCT CATGCTCTTC ATTTTCATCT TCAGCATCCT TGGGATGCAT 
ATCTTTGGCT GCAAATTCAG CCTCCGCACG GACACTGGAG ACACAGTCCC TGACAGGAAG 
AACTTCGATT CCTTACTGTG GGCCATCGTC ACCGTGTTTC AGATCCTCAC TCAGGAGGAC 
TGGAACGTTG TCCTGTACAA TGGCATGGCC TCCACCACCC CCTGGGCCTC CCTCTATTTT 
GTTGCCCTCA TGACCTTTGG CAACTATGTT CTCTTCAATC TCCTGGTGGC TATCCTGGTC 
GAGGGCTTCC AGGCTGAGGG TGATGCTAAT CGCTCCTACT CAGACGAGGA CCAGAGCTCA 
TCCAACTTGG AGGAGCTTGA TAAGCTCCCA GAGGGCCTGG ACAGCAGCAG AGATCTCAAG 
CTCTGCCCAA TACCCATGAC ACCCAATGGA CACCTGGACC CTAGTCTCCC TCTGGGTGGA 
CATCTGGGTC CTGCTGGTGC CATGGGTGCT GCCCCCCGCC TCTCACTGCA GCCAGACCCA 
GTGCTGGTGG CCCTAGAATC CCGGAAAAGC AGTGTCATGT CACTGGGCAG GATGAGCTAT 
GATCAGCGAT CTTTGTCCAG CTCCCGGAGC TCCTACTATG GGCCCTGGGG CCGCAGCGGG 
ACCTGGGCCA GCCGCCGCTC CAGCTGGAAC AGCCTGAAAC ACAAGCCACC CTCAGCTGAG 
CACGAGTCCC TGCTCTCCGG GGAGCGTGGT GGTAGTTGCG TCAGGGCCTG TGAAGGCGCC 
CGGGAGGATG CGCCACCTCG CGCTGCACCC CTGCATGCCC CGCACACCCA CCACGCGCAT 
CATGGACCCC ACTTGGCACA CCGTCACCGC CACCACCGTC GGACACTGTC CCTTGATACC 
AGGGACTCTG TTGACCTGGC AGAGCTGGTG CCCGTGGTGG GTGCCCACTC GCGGGCCGCT 
TGGAGGGCGG CAGGCCAGGC CCCTGGGCAC GAGGACTGCA ATGGCAGAAT GCCCAACATA 
GCCAAGGATG TCTTCACCAA GATGGACGAC CGCCGCGACC GTGGGGAGGA CGAGGAGGAG 
ATCGACTACA CCCTGTGTTT CCGCGTCCGC AAGATGATTG ATGTGTACAA GCCTGACTGG 
TGCGAAGTCC GCGAGGACTG GTCGGTCTAC CTCTTCTCCC CTGAGAACAA GTTCCGGATC 
CTGTGTCAGA CCATTATTGC TCACAAGCTT TTTGACTACG TGGTCTTGGC CTTCATCTTC 
CTCAACTGTA TCACCATTGC TCTGGAGAGG CCCCAGATCG AAGCTGGTAG CACTGAGCGC 
ATCTTCCTCA CGGTGTCTAA CTACATCTTC ACAGCCATCT TTGTGGGCGA GATGACACTG 
AAGGTGGTCT CTTTGGGCCT ATACTTCGGT GAGCAAGCAT ACTTGCGTAG CAGCTGGAAC 
GTCCTGGATG GTTTCCTGGT CTTTGTGTCT ATCATCGATA TCGTGGTATC CGTGGCCTCT 
GCAGGGGGAG CCAAGATCCT GGGGGTCCTC CGGGTCCTGC GGCTCCTGCG TACCTTACGT 
CCTTTGAGGG TCATCAGCCG GGCCCCTGGG CTGAAGCTGG TGGTAGAGAC GCTCATCTCC 
TCCCTCAAGC CCATTGGAAA CATTGTCCTC ATCTGCTGCG CCTTCTTCAT CATCTTCGGT 
ATCCTGGGGG TGCAGCTTTT CAAAGGCAAG TTCTATCACT GTCTGGGAGT GGACACCCGA 
AACATCACCA ACCGATCAGA CTGTGTGGCC GCCAACTACC GCTGGGTGCA TCACAAATAC 
AACTTTGATA ACCTGGGCCA GGCTTTGATG TCCCTCTTTG TCTTGGCCTC CAAGGATGGC 
TGGGTGAACA TCATGTATAA TGGATTGGAT GCTGTTGCTG TGGACCAGCA GCCGGTGACA 
AACCACAACC CCTGGATGCT GCTGTACTTC ATCTCCTTCC TGCTCATCGT CAGCTTCTTC 
GTGCTCAACA TGTTCGTGGG AGTGGTCGTG GAGAACTTCC ACAAGTGCCG GCAGCATCAG 
GAGGCTGAGG AGGCACGGCG GCGTGAGGAG AAACGGCTAC GGCGCCTGGA AAAGAAGCGC 
CGTAAGGCTC AGAGGCTGCC CTACTATGCC ACCTACTGTC CCACAAGACT GCTCATCCAC 
TCCATGTGCA CTAGCCACTA CCTGGACATC TTCATCACCT TCATCATCTG CCTCAACGTG 
GTCACCATGT CCCTGGAGCA CTACAACCAG CCTACATCCC TAGAGACAGC CCTCAAGTAC 
TGCAACTACA TGTTCACCAC TGTCTTTGTG CTGGAGGCTG TGCTGAAGCT GGTGGCGTTT 
GGCCTGAGGC GTTTCTTCAA GGACCGATGG AACCAGCTGG ACTTGGCCAT TGTGCTGCTG 
TCTGTCATGG GCATCACACT GGAGGAGATT GAGATCAATG CGGCCCTGCC CATCAACCCC 
ACCATCATCC GTATCATGCG TGTTCTGCGT ATTGCCCGGG TGTTGAAGCT GTTGAAGATG 
GCCACAGGAA TGCGGGCCCT GCTGGACACA GTGGTACAGG CTCTGCCCCA GGTGGGCAAC 
CTGGGCCTCC TCTTCATGCT GCTCTTCTTC ATCTACGCCG CTCTGGGAGT GGAGCTCTTC 
GGAAAGCTGG TCTGCAATGA CGAGAACCCG TGTGAGGGCA TGAGCCGGCA CGCCACCTTC 
GAGAACTTCG GCATGGCCTT CCTCACACTC TTCCAGGTCT CCACAGGCGA TAACTGGAAT 
GGGATTATGA AGGACACTCT GCGAGACTGC ACCCACGACG AGCGCAGCTG CCTGAGCAGC 
CTGCAGTTTG TGTCGCCTCT CTACTTTGTG AGCTTCGTAC TCACAGCGCA GTTTGTGCTC 
ATCAACGTGG TGGTGGCCGT GCTGATGAAA CATCTGGATG ACAGCAACAA GGAGGCCCAG 
GAGGATGCAG AGATGGATGC TGAGATCGAG CTGGAGATGG CCCATGGCCT AGGCCCCGGG 
CCCGGCCCCT GTCCCTGTCC CTGTCCCTGC CCCTGCCCCT GTCCCTGTCC TGGCCCGAGG 
ATGCCCACCA GTTCACCGGG GGCTCCAGGC CGAGGATCAG GAGGGGCAGG CGTTGGAGGC 
GACACTGAGA GCCACCTGTG CCGGCACTGC TATTCTCCAG CCCAGGAGAC CCTGTGGCTG 
GACAGCGTCT CTTTAATCAT CAAGGACTCC TTGGAGGGGG AGCTGACCAT CATTGACAAC 
CTGTCTGGCT CCATCTTCCA CCACTACTCC TCACCTGCCG GCTGTGACAA GTGTCACCAT 
GACAAGCAAG AGGTGCAGCT GGCTGAGACA GAGGCCTTCT CCCTGAACTC AGACAGGTCC 
TCGTCCGTCC TGCTGGGGGA TGACCTGAGT CTTGAGGACC CCACGGCCTG CCCGCAGGGC 
CCCAAGGAGA GCAAGGGTGA GCTAGAGCCT CCGGAGCCCA TGCAGGCTGG AGACCTGGAT 
GAATGCTTCT TCCCCTTTGC CGGTGAGCCA GTGTCTGCAG GCCCAGAGAG CCTGCTGTGC 
GAGATGGGGG CCATTCCATT CAACCCCGTC CAGTCCTGGC TCAAACATGA GAGCAACCAA 
GCACCCCCAA GCCCTTTCTC CCCGGATGGT TCCAGCCCTC TGCTGCAGAT GCCTGCTGAG 
TTCTTCCACC CTGCTGTGTC TGCCAGCCAG AAGGGGCAGG AACCGGGCAT GAGTTCAGGG 
ACCCTGCCCA AGATTGCACT TCAGGGGTCC TGGGCATCCC TGAGGTCACC GAGTGTCAAC 
TGCACCCTCT TGCGCCAGGC TACTGTGAGT GACACGTCTT TGGATGCCAG CCCTAGCAGC 
TCAGCGGGCA GCCTGCAGAC CACACTGGAG GACAGTCTGA CCCTGAGTGA CAGTCCTCGG 
CGTGCCCTGG GACCACCAGT CCAGGTGCCA GGGCCACGGG CTAGCCTGTC ACCTGCCACC 
CGGCGCCGCC TCAGCCTGCG GGGGCGTGGC CTGTTTAGTC TGCGTGGGCT GCGGGCCCAT 
CAGCGTAGCC ACAGCAGCGG TGGCTCCACC AGCCCTGGCT GCACTTACCA CGACTCCATG 
GACCCCTCTG ATGAGGAGGG CCGCGGGGGC GCAGGTGGTG GGGGCGCGGG CAGTGAGCAC 
TCCGAGACCC TCAGCAGCCT GTCACTCACA TCTCTCTTCT GCCTACCACC CACGCTGCCA 
CCACCCGGTC TCACCCCAGC CAGGAAGTTC AGCAGTACCA GCAGCCTGGC TGCTGGACCT 
GGCCGTCCTG GCGCCACTGT CTCGGTCCGC GGCCTGGCCA GGAGTCCCTC ATGGGCTGCA 
GACCGCAGCA AGGACCCACC AGGCCAGGCA CAGCTGGCCT CCGGCTTCGG CTCCTCAGCA 
CCCGAGCCAC AGCCACCTCC CGGGGAGTCA ACGGATGCGG CGAGCAAAAG GAAGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001037773.2
CDS	562..7161
Translation	MADSNLPPSSSAAPDPEPGITEQPGPRSPPPSPPGLEEPLDGTNPDVPHPDLAPVAFFCLRQTTSPRNWCIKMVCNPWFECVSMLVILLNCVTLGMYQPCDDMECLSDRCKILQVFDDFIFIFFAMEMVLKMVALGIFGKKCYLGDTWNRLDFFIVMAGMVEYSLDLQNINLSAIRTVRVLRPLKAINRVPSMRILVNLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLWAGLLRNRCFLEENFTIQGDVALPPYYQPEEDDEMPFICSLSGDNGIMGCHEIPPLKEQGRECCLSKDDMYDFGAGRQDLNASGLCVNWNRYYNVCRTGNANPHKGAINFDNIGYAWIVIFQVITLEGWVEIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQREHRLMLEQRQRYLSSSTVASYAEPGDCYEEIFQYVCHILRKAKRRALGLYQALQNRRQATGPGTPAPAKPGPHAKEPSHCKLCPRHSPLDTTPHTLVQPISAILASDPSSCPRCQHEAGRRPSGLGSTDSGQEGSGSGGSAEAEANGDGPQSSEDGVSSGLGKEEEQEDGAARLCGDVWRETRAKLRGIVDSKYFNRGIMMAILVNTVSMGIEHHEQPEELTNILEICNVVFTSMFALEMILKLAAFGLFDYLRNPYNIFDSIIVIISIWEIVGQADGGLSVLRTFRLLRVLKLVRFMPALRRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHIFGCKFSLRTDTGDTVPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMASTTPWASLYFVALMTFGNYVLFNLLVAILVEGFQAEGDANRSYSDEDQSSSNLEELDKLPEGLDSSRDLKLCPIPMTPNGHLDPSLPLGGHLGPAGAMGAAPRLSLQPDPVLVALESRKSSVMSLGRMSYDQRSLSSSRSSYYGPWGRSGTWASRRSSWNSLKHKPPSAEHESLLSGERGGSCVRACEGAREDAPPRAAPLHAPHTHHAHHGPHLAHRHRHHRRTLSLDTRDSVDLAELVPVVGAHSRAAWRAAGQAPGHEDCNGRMPNIAKDVFTKMDDRRDRGEDEEEIDYTLCFRVRKMIDVYKPDWCEVREDWSVYLFSPENKFRILCQTIIAHKLFDYVVLAFIFLNCITIALERPQIEAGSTERIFLTVSNYIFTAIFVGEMTLKVVSLGLYFGEQAYLRSSWNVLDGFLVFVSIIDIVVSVASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLKPIGNIVLICCAFFIIFGILGVQLFKGKFYHCLGVDTRNITNRSDCVAANYRWVHHKYNFDNLGQALMSLFVLASKDGWVNIMYNGLDAVAVDQQPVTNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEAEEARRREEKRLRRLEKKRRKAQRLPYYATYCPTRLLIHSMCTSHYLDIFITFIICLNVVTMSLEHYNQPTSLETALKYCNYMFTTVFVLEAVLKLVAFGLRRFFKDRWNQLDLAIVLLSVMGITLEEIEINAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGKLVCNDENPCEGMSRHATFENFGMAFLTLFQVSTGDNWNGIMKDTLRDCTHDERSCLSSLQFVSPLYFVSFVLTAQFVLINVVVAVLMKHLDDSNKEAQEDAEMDAEIELEMAHGLGPGPGPCPCPCPCPCPCPCPGPRMPTSSPGAPGRGSGGAGVGGDTESHLCRHCYSPAQETLWLDSVSLIIKDSLEGELTIIDNLSGSIFHHYSSPAGCDKCHHDKQEVQLAETEAFSLNSDRSSSVLLGDDLSLEDPTACPQGPKESKGELEPPEPMQAGDLDECFFPFAGEPVSAGPESLLCEMGAIPFNPVQSWLKHESNQAPPSPFSPDGSSPLLQMPAEFFHPAVSASQKGQEPGMSSGTLPKIALQGSWASLRSPSVNCTLLRQATVSDTSLDASPSSSAGSLQTTLEDSLTLSDSPRRALGPPVQVPGPRASLSPATRRRLSLRGRGLFSLRGLRAHQRSHSSGGSTSPGCTYHDSMDPSDEEGRGGAGGGGAGSEHSETLSSLSLTSLFCLPPTLPPPGLTPARKFSSTSSLAAGPGRPGATVSVRGLARSPSWAADRSKDPPGQAQLASGFGSSAPEPQPPPGESTDAASKRKR

Target ORF information:

RefSeq Version	NM_001044308.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, alpha 1I subunit (Cacna1i), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001044308.2

ORF Insert Sequence:

ATGGCTGACA GCAACTTACC ACCCTCATCT TCAGCAGCCC CGGACCCTGA GCCGGGAATC 
ACTGAGCAGC CGGGGCCCCG GAGTCCCCCT CCATCCCCTC CAGGCCTGGA GGAGCCATTG 
GATGGAACCA ACCCTGACGT CCCGCATCCA GACCTGGCAC CCGTTGCCTT CTTCTGCCTG 
CGCCAGACCA CGAGCCCACG GAACTGGTGC ATCAAGATGG TATGTAACCC GTGGTTCGAG 
TGTGTGAGCA TGCTGGTCAT CCTGCTGAAC TGTGTGACCC TGGGCATGTA CCAGCCATGT 
GACGATATGG AGTGCCTCTC AGACCGCTGC AAGATCCTGC AGGTCTTTGA TGACTTCATC 
TTCATCTTCT TCGCCATGGA GATGGTGCTT AAGATGGTGG CCCTGGGCAT TTTTGGCAAG 
AAGTGCTACC TCGGGGACAC ATGGAACCGC TTGGACTTCT TCATTGTCAT GGCAGGGATG 
GTCGAGTACT CTCTGGACCT ACAGAACATC AACCTGTCAG CCATCCGCAC TGTGCGTGTC 
CTGAGGCCTC TCAAAGCCAT CAACCGTGTT CCCAGCATGC GGATTCTGGT GAACCTGCTG 
CTGGACACGC TGCCCATGCT GGGAAACGTG CTCCTGCTCT GTTTCTTCGT CTTCTTCATC 
TTTGGCATCA TCGGCGTGCA GCTCTGGGCA GGCCTGCTGC GGAACCGCTG CTTCCTGGAG 
GAGAACTTCA CCATACAAGG GGATGTGGCC CTGCCCCCTT ACTACCAACC AGAAGAGGAC 
GACGAGATGC CCTTCATCTG CTCCCTGTCG GGGGACAATG GCATTATGGG CTGTCACGAG 
ATCCCTCCAC TCAAGGAGCA GGGCCGAGAG TGCTGCCTGT CCAAAGATGA CATGTACGAC 
TTCGGGGCGG GACGCCAAGA CCTCAACGCC AGCGGCCTCT GCGTCAACTG GAACCGCTAC 
TACAACGTCT GCCGCACGGG CAACGCCAAC CCTCACAAGG GCGCCATCAA CTTTGACAAC 
ATTGGCTATG CCTGGATCGT GATCTTTCAG GTGATCACTC TGGAAGGCTG GGTGGAGATC 
ATGTACTACG TGATGGATGC ACATTCTTTC TACAACTTCA TCTACTTCAT CCTGCTCATC 
ATAGTGGGCT CCTTCTTCAT GATCAACCTG TGCCTCGTTG TCATAGCGAC CCAGTTCTCT 
GAGACCAAGC AACGGGAGCA CCGGCTGATG CTGGAGCAAC GCCAGCGCTA CCTGTCCTCC 
AGCACGGTGG CCAGTTACGC CGAGCCCGGC GACTGCTATG AGGAGATCTT CCAATATGTC 
TGTCACATCC TTCGCAAGGC CAAGCGCCGT GCTCTAGGCC TCTACCAGGC CCTGCAGAAC 
CGGCGCCAGG CCACGGGCCC CGGGACACCA GCCCCTGCCA AGCCTGGGCC CCATGCCAAG 
GAGCCCAGCC ACTGCAAGCT GTGCCCACGA CACAGTCCCC TGGACACCAC TCCCCACACA 
CTGGTGCAGC CCATCTCTGC CATTCTGGCC TCCGATCCCA GCAGCTGCCC TCGCTGCCAG 
CACGAGGCAG GCAGGCGGCC CTCTGGCCTG GGCAGCACTG ACTCGGGCCA GGAAGGCTCA 
GGTTCTGGCG GTTCTGCAGA GGCTGAAGCC AATGGCGATG GACCCCAAAG CAGTGAGGAT 
GGGGTCTCCT CAGGTTTGGG GAAGGAGGAG GAACAGGAGG ACGGGGCAGC CCGACTGTGT 
GGGGATGTGT GGCGAGAGAC ACGGGCCAAG CTGCGGGGCA TTGTGGACAG CAAGTACTTC 
AACAGAGGCA TCATGATGGC TATTCTGGTT AACACTGTCA GCATGGGCAT CGAGCACCAT 
GAACAGCCAG AGGAACTGAC CAACATCCTG GAGATCTGCA ACGTGGTCTT CACCAGCATG 
TTTGCCCTGG AGATGATTCT GAAGCTGGCT GCCTTTGGGC TCTTTGACTA CCTGCGGAAC 
CCTTACAACA TCTTCGACAG CATCATTGTC ATCATCAGCA TCTGGGAAAT CGTGGGGCAG 
GCTGACGGCG GCCTGTCCGT GCTGCGCACC TTCCGGCTGC TGCGGGTGCT GAAGCTGGTG 
CGCTTCATGC CGGCGCTGCG GCGCCAGCTC GTGGTGCTCA TGAAGACCAT GGACAACGTG 
GCCACCTTCT GCATGCTGCT CATGCTCTTC ATTTTCATCT TCAGCATCCT TGGGATGCAT 
ATCTTTGGCT GCAAATTCAG CCTCCGCACG GACACTGGAG ACACAGTCCC TGACAGGAAG 
AACTTCGATT CCTTACTGTG GGCCATCGTC ACCGTGTTTC AGATCCTCAC TCAGGAGGAC 
TGGAACGTTG TCCTGTACAA TGGCATGGCC TCCACCACCC CCTGGGCCTC CCTCTATTTT 
GTTGCCCTCA TGACCTTTGG CAACTATGTT CTCTTCAATC TCCTGGTGGC TATCCTGGTC 
GAGGGCTTCC AGGCTGAGGG TGATGCTAAT CGCTCCTACT CAGACGAGGA CCAGAGCTCA 
TCCAACTTGG AGGAGCTTGA TAAGCTCCCA GAGGGCCTGG ACAGCAGCAG AGATCTCAAG 
CTCTGCCCAA TACCCATGAC ACCCAATGGA CACCTGGACC CTAGTCTCCC TCTGGGTGGA 
CATCTGGGTC CTGCTGGTGC CATGGGTGCT GCCCCCCGCC TCTCACTGCA GCCAGACCCA 
GTGCTGGTGG CCCTAGAATC CCGGAAAAGC AGTGTCATGT CACTGGGCAG GATGAGCTAT 
GATCAGCGAT CTTTGTCCAG CTCCCGGAGC TCCTACTATG GGCCCTGGGG CCGCAGCGGG 
ACCTGGGCCA GCCGCCGCTC CAGCTGGAAC AGCCTGAAAC ACAAGCCACC CTCAGCTGAG 
CACGAGTCCC TGCTCTCCGG GGAGCGTGGT GGTAGTTGCG TCAGGGCCTG TGAAGGCGCC 
CGGGAGGATG CGCCACCTCG CGCTGCACCC CTGCATGCCC CGCACACCCA CCACGCGCAT 
CATGGACCCC ACTTGGCACA CCGTCACCGC CACCACCGTC GGACACTGTC CCTTGATACC 
AGGGACTCTG TTGACCTGGC AGAGCTGGTG CCCGTGGTGG GTGCCCACTC GCGGGCCGCT 
TGGAGGGCGG CAGGCCAGGC CCCTGGGCAC GAGGACTGCA ATGGCAGAAT GCCCAACATA 
GCCAAGGATG TCTTCACCAA GATGGACGAC CGCCGCGACC GTGGGGAGGA CGAGGAGGAG 
ATCGACTACA CCCTGTGTTT CCGCGTCCGC AAGATGATTG ATGTGTACAA GCCTGACTGG 
TGCGAAGTCC GCGAGGACTG GTCGGTCTAC CTCTTCTCCC CTGAGAACAA GTTCCGGATC 
CTGTGTCAGA CCATTATTGC TCACAAGCTT TTTGACTACG TGGTCTTGGC CTTCATCTTC 
CTCAACTGTA TCACCATTGC TCTGGAGAGG CCCCAGATCG AAGCTGGTAG CACTGAGCGC 
ATCTTCCTCA CGGTGTCTAA CTACATCTTC ACAGCCATCT TTGTGGGCGA GATGACACTG 
AAGGTGGTCT CTTTGGGCCT ATACTTCGGT GAGCAAGCAT ACTTGCGTAG CAGCTGGAAC 
GTCCTGGATG GTTTCCTGGT CTTTGTGTCT ATCATCGATA TCGTGGTATC CGTGGCCTCT 
GCAGGGGGAG CCAAGATCCT GGGGGTCCTC CGGGTCCTGC GGCTCCTGCG TACCTTACGT 
CCTTTGAGGG TCATCAGCCG GGCCCCTGGG CTGAAGCTGG TGGTAGAGAC GCTCATCTCC 
TCCCTCAAGC CCATTGGAAA CATTGTCCTC ATCTGCTGCG CCTTCTTCAT CATCTTCGGT 
ATCCTGGGGG TGCAGCTTTT CAAAGGCAAG TTCTATCACT GTCTGGGAGT GGACACCCGA 
AACATCACCA ACCGATCAGA CTGTGTGGCC GCCAACTACC GCTGGGTGCA TCACAAATAC 
AACTTTGATA ACCTGGGCCA GGCTTTGATG TCCCTCTTTG TCTTGGCCTC CAAGGATGGC 
TGGGTGAACA TCATGTATAA TGGATTGGAT GCTGTTGCTG TGGACCAGCA GCCGGTGACA 
AACCACAACC CCTGGATGCT GCTGTACTTC ATCTCCTTCC TGCTCATCGT CAGCTTCTTC 
GTGCTCAACA TGTTCGTGGG AGTGGTCGTG GAGAACTTCC ACAAGTGCCG GCAGCATCAG 
GAGGCTGAGG AGGCACGGCG GCGTGAGGAG AAACGGCTAC GGCGCCTGGA AAAGAAGCGC 
CGTAAGGCTC AGAGGCTGCC CTACTATGCC ACCTACTGTC CCACAAGACT GCTCATCCAC 
TCCATGTGCA CTAGCCACTA CCTGGACATC TTCATCACCT TCATCATCTG CCTCAACGTG 
GTCACCATGT CCCTGGAGCA CTACAACCAG CCTACATCCC TAGAGACAGC CCTCAAGTAC 
TGCAACTACA TGTTCACCAC TGTCTTTGTG CTGGAGGCTG TGCTGAAGCT GGTGGCGTTT 
GGCCTGAGGC GTTTCTTCAA GGACCGATGG AACCAGCTGG ACTTGGCCAT TGTGCTGCTG 
TCTGTCATGG GCATCACACT GGAGGAGATT GAGATCAATG CGGCCCTGCC CATCAACCCC 
ACCATCATCC GTATCATGCG TGTTCTGCGT ATTGCCCGGG TGTTGAAGCT GTTGAAGATG 
GCCACAGGAA TGCGGGCCCT GCTGGACACA GTGGTACAGG CTCTGCCCCA GGTGGGCAAC 
CTGGGCCTCC TCTTCATGCT GCTCTTCTTC ATCTACGCCG CTCTGGGAGT GGAGCTCTTC 
GGAAAGCTGG TCTGCAATGA CGAGAACCCG TGTGAGGGCA TGAGCCGGCA CGCCACCTTC 
GAGAACTTCG GCATGGCCTT CCTCACACTC TTCCAGGTCT CCACAGGCGA TAACTGGAAT 
GGGATTATGA AGGACACTCT GCGAGACTGC ACCCACGACG AGCGCAGCTG CCTGAGCAGC 
CTGCAGTTTG TGTCGCCTCT CTACTTTGTG AGCTTCGTAC TCACAGCGCA GTTTGTGCTC 
ATCAACGTGG TGGTGGCCGT GCTGATGAAA CATCTGGATG ACAGCAACAA GGAGGCCCAG 
GAGGATGCAG AGATGGATGC TGAGATCGAG CTGGAGATGG CCCATGGCCT AGGCCCCGGG 
CCCGGCCCCT GTCCCTGTCC CTGTCCCTGC CCCTGCCCCT GTCCCTGTCC TGGCCCGAGG 
ATGCCCACCA GTTCACCGGG GGCTCCAGGC CGAGGATCAG GAGGGGCAGG CGTTGGAGGC 
GACACTGAGA GCCACCTGTG CCGGCACTGC TATTCTCCAG CCCAGGAGAC CCTGTGGCTG 
GACAGCGTCT CTTTAATCAT CAAGGACTCC TTGGAGGGGG AGCTGACCAT CATTGACAAC 
CTGTCTGGCT CCATCTTCCA CCACTACTCC TCACCTGCCG GCTGTGACAA GTGTCACCAT 
GACAAGCAAG AGGTGCAGCT GGCTGAGACA GAGGCCTTCT CCCTGAACTC AGACAGGTCC 
TCGTCCGTCC TGCTGGGGGA TGACCTGAGT CTTGAGGACC CCACGGCCTG CCCGCAGGGC 
CCCAAGGAGA GCAAGGGTGA GCTAGAGCCT CCGGAGCCCA TGCAGGCTGG AGACCTGGAT 
GAATGCTTCT TCCCCTTTGC CGGTGAGCCA GTGTCTGCAG GCCCAGAGAG CCTGCTGTGC 
GAGATGGGGG CCATTCCATT CAACCCCGTC CAGTCCTGGC TCAAACATGA GAGCAACCAA 
GCACCCCCAA GCCCTTTCTC CCCGGATGGT TCCAGCCCTC TGCTGCAGAT GCCTGCTGAG 
TTCTTCCACC CTGCTGTGTC TGCCAGCCAG AAGGGGCAGG AACCGGGCAT GAGTTCAGGG 
ACCCTGCCCA AGATTGCACT TCAGGGGTCC TGGGCATCCC TGAGGTCACC GAGTGTCAAC 
TGCACCCTCT TGCGCCAGGC TACTGTGAGT GACACGTCTT TGGATGCCAG CCCTAGCAGC 
TCAGCGGGCA GCCTGCAGAC CACACTGGAG GACAGTCTGA CCCTGAGTGA CAGTCCTCGG 
CGTGCCCTGG GACCACCAGT CCAGGTGCCA GGGCCACGGG CTAGCCTGTC ACCTGCCACC 
CGGCGCCGCC TCAGCCTGCG GGGGCGTGGC CTGTTTAGTC TGCGTGGGCT GCGGGCCCAT 
CAGCGTAGCC ACAGCAGCGG TGGCTCCACC AGCCCTGGCT GCACTTACCA CGACTCCATG 
GACCCCTCTG ATGAGGAGGG CCGCGGGGGC GCAGGTGGTG GGGGCGCGGG CAGTGAGCAC 
TCCGAGACCC TCAGCAGCCT GTCACTCACA TCTCTCTTCT GCCTACCACC CACGCTGCCA 
CCACCCGGTC TCACCCCAGC CAGGAAGTTC AGCAGTACCA GCAGCCTGGC TGCTGGACCT 
GGCCGTCCTG GCGCCACTGT CTCGGTCCGC GGCCTGGCCA GGAGTCCCTC ATGGGCTGCA 
GACCGCAGCA AGGACCCACC AGGCCAGGCA CAGCTGGCCT CCGGCTTCGG CTCCTCAGCA 
CCCGAGCCAC AGCCACCTCC CGGGGAGTCA ACGGATGCGG CGAGCAAAAG GAAGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1i ( XM_006520949.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006521012.1 Mus musculus calcium channel, voltage-dependent, alpha 1I subunit (Cacna1i), transcript variant X1, mRNA.

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CloneID	OMu51299
Clone ID Related Accession (Same CDS sequence)	XM_006520949.2 , XM_006520949.1
Accession Version	XM_006520949.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1I isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGACA GCAACTTACC ACCCTCATCT TCAGCAGCCC CGGACCCTGA GCCGGGAATC 
ACTGAGCAGC CGGGGCCCCG GAGTCCCCCT CCATCCCCTC CAGGCCTGGA GGAGCCATTG 
GATGGAACCA ACCCTGACGT CCCGCATCCA GACCTGGCAC CCGTTGCCTT CTTCTGCCTG 
CGCCAGACCA CGAGCCCACG GAACTGGTGC ATCAAGATGG TATGTAACCC GTGGTTCGAG 
TGTGTGAGCA TGCTGGTCAT CCTGCTGAAC TGTGTGACCC TGGGCATGTA CCAGCCATGT 
GACGATATGG AGTGCCTCTC AGACCGCTGC AAGATCCTGC AGGTCTTTGA TGACTTCATC 
TTCATCTTCT TCGCCATGGA GATGGTGCTT AAGATGGTGG CCCTGGGCAT TTTTGGCAAG 
AAGTGCTACC TCGGGGACAC ATGGAACCGC TTGGACTTCT TCATTGTCAT GGCAGGGATG 
GTCGAGTACT CTCTGGACCT ACAGAACATC AACCTGTCAG CCATCCGCAC TGTGCGTGTC 
CTGAGGCCTC TCAAAGCCAT CAACCGTGTT CCCAGCATGC GGATTCTGGT GAACCTGCTG 
CTGGACACGC TGCCCATGCT GGGAAACGTG CTCCTGCTCT GTTTCTTCGT CTTCTTCATC 
TTTGGCATCA TCGGCGTGCA GCTCTGGGCA GGCCTGCTGC GGAACCGCTG CTTCCTGGAG 
GAGAACTTCA CCATACAAGG GGATGTGGCC CTGCCCCCTT ACTACCAACC AGAAGAGGAC 
GACGAGATGC CCTTCATCTG CTCCCTGTCG GGGGACAATG GCATTATGGG CTGTCACGAG 
ATCCCTCCAC TCAAGGAGCA GGGCCGAGAG TGCTGCCTGT CCAAAGATGA CATGTACGAC 
TTCGGGGCGG GACGCCAAGA CCTCAACGCC AGCGGCCTCT GCGTCAACTG GAACCGCTAC 
TACAACGTCT GCCGCACGGG CAACGCCAAC CCTCACAAGG GCGCCATCAA CTTTGACAAC 
ATTGGCTATG CCTGGATCGT GATCTTTCAG GTGATCACTC TGGAAGGCTG GGTGGAGATC 
ATGTACTACG TGATGGATGC ACATTCTTTC TACAACTTCA TCTACTTCAT CCTGCTCATC 
ATAGTGGGCT CCTTCTTCAT GATCAACCTG TGCCTCGTTG TCATAGCGAC CCAGTTCTCT 
GAGACCAAGC AACGGGAGCA CCGGCTGATG CTGGAGCAAC GCCAGCGCTA CCTGTCCTCC 
AGCACGGTGG CCAGTTACGC CGAGCCCGGC GACTGCTATG AGGAGATCTT CCAATATGTC 
TGTCACATCC TTCGCAAGGC CAAGCGCCGT GCTCTAGGCC TCTACCAGGC CCTGCAGAAC 
CGGCGCCAGG CCACGGGCCC CGGGACACCA GCCCCTGCCA AGCCTGGGCC CCATGCCAAG 
GAGCCCAGCC ACTGCAAGCT GTGCCCACGA CACAGTCCCC TGGACACCAC TCCCCACACA 
CTGGTGCAGC CCATCTCTGC CATTCTGGCC TCCGATCCCA GCAGCTGCCC TCGCTGCCAG 
CACGAGGCAG GCAGGCGGCC CTCTGGCCTG GGCAGCACTG ACTCGGGCCA GGAAGGCTCA 
GGTTCTGGCG GTTCTGCAGA GGCTGAAGCC AATGGCGATG GACCCCAAAG CAGTGAGGAT 
GGGGTCTCCT CAGGTTTGGG GAAGGAGGAG GAACAGGAGG ACGGGGCAGC CCGACTGTGT 
GGGGATGTGT GGCGAGAGAC ACGGGCCAAG CTGCGGGGCA TTGTGGACAG CAAGTACTTC 
AACAGAGGCA TCATGATGGC TATTCTGGTT AACACTGTCA GCATGGGCAT CGAGCACCAT 
GAACAGCCAG AGGAACTGAC CAACATCCTG GAGATCTGCA ACGTGGTCTT CACCAGCATG 
TTTGCCCTGG AGATGATTCT GAAGCTGGCT GCCTTTGGGC TCTTTGACTA CCTGCGGAAC 
CCTTACAACA TCTTCGACAG CATCATTGTC ATCATCAGCA TCTGGGAAAT CGTGGGGCAG 
GCTGACGGCG GCCTGTCCGT GCTGCGCACC TTCCGGCTGC TGCGGGTGCT GAAGCTGGTG 
CGCTTCATGC CGGCGCTGCG GCGCCAGCTC GTGGTGCTCA TGAAGACCAT GGACAACGTG 
GCCACCTTCT GCATGCTGCT CATGCTCTTC ATTTTCATCT TCAGCATCCT TGGGATGCAT 
ATCTTTGGCT GCAAATTCAG CCTCCGCACG GACACTGGAG ACACAGTCCC TGACAGGAAG 
AACTTCGATT CCTTACTGTG GGCCATCGTC ACCGTGTTTC AGATCCTCAC TCAGGAGGAC 
TGGAACGTTG TCCTGTACAA TGGCATGGCC TCCACCACCC CCTGGGCCTC CCTCTATTTT 
GTTGCCCTCA TGACCTTTGG CAACTATGTT CTCTTCAATC TCCTGGTGGC TATCCTGGTC 
GAGGGCTTCC AGGCTGAGGG TGATGCTAAT CGCTCCTACT CAGACGAGGA CCAGAGCTCA 
TCCAACTTGG AGGAGCTTGA TAAGCTCCCA GAGGGCCTGG ACAGCAGCAG AGATCTCAAG 
CTCTGCCCAA TACCCATGAC ACCCAATGGA CACCTGGACC CTAGTCTCCC TCTGGGTGGA 
CATCTGGGTC CTGCTGGTGC CATGGGTGCT GCCCCCCGCC TCTCACTGCA GCCAGACCCA 
GTGCTGGTGG CCCTAGAATC CCGGAAAAGC AGTGTCATGT CACTGGGCAG GATGAGCTAT 
GATCAGCGAT CTTTGTCCAG CTCCCGGAGC TCCTACTATG GGCCCTGGGG CCGCAGCGGG 
ACCTGGGCCA GCCGCCGCTC CAGCTGGAAC AGCCTGAAAC ACAAGCCACC CTCAGCTGAG 
CACGAGTCCC TGCTCTCCGG GGAGCGTGGT GGTAGTTGCG TCAGGGCCTG TGAAGGCGCC 
CGGGAGGATG CGCCACCTCG CGCTGCACCC CTGCATGCCC CGCACACCCA CCACGCGCAT 
CATGGACCCC ACTTGGCACA CCGTCACCGC CACCACCGTC GGACACTGTC CCTTGATACC 
AGGGACTCTG TTGACCTGGC AGAGCTGGTG CCCGTGGTGG GTGCCCACTC GCGGGCCGCT 
TGGAGGGCGG CAGGCCAGGC CCCTGGGCAC GAGGACTGCA ATGGCAGAAT GCCCAACATA 
GCCAAGGATG TCTTCACCAA GATGGACGAC CGCCGCGACC GTGGGGAGGA CGAGGAGGAG 
ATCGACTACA CCCTGTGTTT CCGCGTCCGC AAGATGATTG ATGTGTACAA GCCTGACTGG 
TGCGAAGTCC GCGAGGACTG GTCGGTCTAC CTCTTCTCCC CTGAGAACAA GTTCCGGATC 
CTGTGTCAGA CCATTATTGC TCACAAGCTT TTTGACTACG TGGTCTTGGC CTTCATCTTC 
CTCAACTGTA TCACCATTGC TCTGGAGAGG CCCCAGATCG AAGCTGGTAG CACTGAGCGC 
ATCTTCCTCA CGGTGTCTAA CTACATCTTC ACAGCCATCT TTGTGGGCGA GATGACACTG 
AAGGTGGTCT CTTTGGGCCT ATACTTCGGT GAGCAAGCAT ACTTGCGTAG CAGCTGGAAC 
GTCCTGGATG GTTTCCTGGT CTTTGTGTCT ATCATCGATA TCGTGGTATC CGTGGCCTCT 
GCAGGGGGAG CCAAGATCCT GGGGGTCCTC CGGGTCCTGC GGCTCCTGCG TACCTTACGT 
CCTTTGAGGG TCATCAGCCG GGCCCCTGGG CTGAAGCTGG TGGTAGAGAC GCTCATCTCC 
TCCCTCAAGC CCATTGGAAA CATTGTCCTC ATCTGCTGCG CCTTCTTCAT CATCTTCGGT 
ATCCTGGGGG TGCAGCTTTT CAAAGGCAAG TTCTATCACT GTCTGGGAGT GGACACCCGA 
AACATCACCA ACCGATCAGA CTGTGTGGCC GCCAACTACC GCTGGGTGCA TCACAAATAC 
AACTTTGATA ACCTGGGCCA GGCTTTGATG TCCCTCTTTG TCTTGGCCTC CAAGGATGGC 
TGGGTGAACA TCATGTATAA TGGATTGGAT GCTGTTGCTG TGGACCAGCA GCCGGTGACA 
AACCACAACC CCTGGATGCT GCTGTACTTC ATCTCCTTCC TGCTCATCGT CAGCTTCTTC 
GTGCTCAACA TGTTCGTGGG AGTGGTCGTG GAGAACTTCC ACAAGTGCCG GCAGCATCAG 
GAGGCTGAGG AGGCACGGCG GCGTGAGGAG AAACGGCTAC GGCGCCTGGA AAAGAAGCGC 
CGTAAGGCTC AGAGGCTGCC CTACTATGCC ACCTACTGTC CCACAAGACT GCTCATCCAC 
TCCATGTGCA CTAGCCACTA CCTGGACATC TTCATCACCT TCATCATCTG CCTCAACGTG 
GTCACCATGT CCCTGGAGCA CTACAACCAG CCTACATCCC TAGAGACAGC CCTCAAGTAC 
TGCAACTACA TGTTCACCAC TGTCTTTGTG CTGGAGGCTG TGCTGAAGCT GGTGGCGTTT 
GGCCTGAGGC GTTTCTTCAA GGACCGATGG AACCAGCTGG ACTTGGCCAT TGTGCTGCTG 
TCTGTCATGG GCATCACACT GGAGGAGATT GAGATCAATG CGGCCCTGCC CATCAACCCC 
ACCATCATCC GTATCATGCG TGTTCTGCGT ATTGCCCGGG TGTTGAAGCT GTTGAAGATG 
GCCACAGGAA TGCGGGCCCT GCTGGACACA GTGGTACAGG CTCTGCCCCA GGTGGGCAAC 
CTGGGCCTCC TCTTCATGCT GCTCTTCTTC ATCTACGCCG CTCTGGGAGT GGAGCTCTTC 
GGAAAGCTGG TCTGCAATGA CGAGAACCCG TGTGAGGGCA TGAGCCGGCA CGCCACCTTC 
GAGAACTTCG GCATGGCCTT CCTCACACTC TTCCAGGTCT CCACAGGCGA TAACTGGAAT 
GGGATTATGA AGGACACTCT GCGAGACTGC ACCCACGACG AGCGCAGCTG CCTGAGCAGC 
CTGCAGTTTG TGTCGCCTCT CTACTTTGTG AGCTTCGTAC TCACAGCGCA GTTTGTGCTC 
ATCAACGTGG TGGTGGCCGT GCTGATGAAA CATCTGGATG ACAGCAACAA GGAGGCCCAG 
GAGGATGCAG AGATGGATGC TGAGATCGAG CTGGAGATGG CCCATGGCCT AGGCCCCGGG 
CCCGGCCCCT GTCCCTGTCC CTGTCCCTGC CCCTGCCCCT GTCCCTGTCC TGGCCCGAGG 
ATGCCCACCA GTTCACCGGG GGCTCCAGGC CGAGGATCAG GAGGGGCAGG CGTTGGAGGC 
GACACTGAGA GCCACCTGTG CCGGCACTGC TATTCTCCAG CCCAGGAGAC CCTGTGGCTG 
GACAGCGTCT CTTTAATCAT CAAGGACTCC TTGGAGGGGG AGCTGACCAT CATTGACAAC 
CTGTCTGGCT CCATCTTCCA CCACTACTCC TCACCTGCCG GCTGTGACAA GTGTCACCAT 
GACAAGCAAG AGGGACCTGT TGCTCAGCGC TCCAGGCCCA GGCTGCCCCA TCCCAGCCCC 
AGGGTGACCC GAGGCCACCA CCTCCCCCCG CAGGTGCAGC TGGCTGAGAC AGAGGCCTTC 
TCCCTGAACT CAGACAGGTC CTCGTCCGTC CTGCTGGGGG ATGACCTGAG TCTTGAGGAC 
CCCACGGCCT GCCCGCAGGG CCCCAAGGAG AGCAAGGGTG AGCTAGAGCC TCCGGAGCCC 
ATGCAGGCTG GAGACCTGGA TGAATGCTTC TTCCCCTTTG CCGGTGAGCC AGTGTCTGCA 
GGCCCAGAGA GCCTGCTGTG CGAGATGGGG GCCATTCCAT TCAACCCCGT CCAGTCCTGG 
CTCAAACATG AGAGCAACCA AGCACCCCCA AGCCCTTTCT CCCCGGATGG TTCCAGCCCT 
CTGCTGCAGA TGCCTGCTGA GTTCTTCCAC CCTGCTGTGT CTGCCAGCCA GAAGGGGCAG 
GAACCGGGCA TGAGTTCAGG GACCCTGCCC AAGATTGCAC TTCAGGGGTC CTGGGCATCC 
CTGAGGTCAC CGAGTGTCAA CTGCACCCTC TTGCGCCAGG CTACTGTGAG TGACACGTCT 
TTGGATGCCA GCCCTAGCAG CTCAGCGGGC AGCCTGCAGA CCACACTGGA GGACAGTCTG 
ACCCTGAGTG ACAGTCCTCG GCGTGCCCTG GGACCACCAG TCCAGGTGCC AGGGCCACGG 
GCTAGCCTGT CACCTGCCAC CCGGCGCCGC CTCAGCCTGC GGGGGCGTGG CCTGTTTAGT 
CTGCGTGGGC TGCGGGCCCA TCAGCGTAGC CACAGCAGCG GTGGCTCCAC CAGCCCTGGC 
TGCACTTACC ACGACTCCAT GGACCCCTCT GATGAGGAGG GCCGCGGGGG CGCAGGTGGT 
GGGGGCGCGG GCAGTGAGCA CTCCGAGACC CTCAGCAGCC TGTCACTCAC ATCTCTCTTC 
TGCCTACCAC CCACGCTGCC ACCACCCGGT CTCACCCCAG CCAGGAAGTT CAGCAGTACC 
AGCAGCCTGG CTGCTGGACC TGGCCGTCCT GGCGCCACTG TCTCGGTCCG CGGCCTGGCC 
AGGAGTCCCT CATGGGCTGC AGACCGCAGC AAGGACCCAC CAGGCCAGGC ACAGCTGGCC 
TCCGGCTTCG GCTCCTCAGC ACCCGAGCCA CAGCCACCTC CCGGGGAGTC AACGGATGCG 
GCGAGCAAAA GGAAGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006521012.1
CDS	234..6914
Translation	MADSNLPPSSSAAPDPEPGITEQPGPRSPPPSPPGLEEPLDGTNPDVPHPDLAPVAFFCLRQTTSPRNWCIKMVCNPWFECVSMLVILLNCVTLGMYQPCDDMECLSDRCKILQVFDDFIFIFFAMEMVLKMVALGIFGKKCYLGDTWNRLDFFIVMAGMVEYSLDLQNINLSAIRTVRVLRPLKAINRVPSMRILVNLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLWAGLLRNRCFLEENFTIQGDVALPPYYQPEEDDEMPFICSLSGDNGIMGCHEIPPLKEQGRECCLSKDDMYDFGAGRQDLNASGLCVNWNRYYNVCRTGNANPHKGAINFDNIGYAWIVIFQVITLEGWVEIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQREHRLMLEQRQRYLSSSTVASYAEPGDCYEEIFQYVCHILRKAKRRALGLYQALQNRRQATGPGTPAPAKPGPHAKEPSHCKLCPRHSPLDTTPHTLVQPISAILASDPSSCPRCQHEAGRRPSGLGSTDSGQEGSGSGGSAEAEANGDGPQSSEDGVSSGLGKEEEQEDGAARLCGDVWRETRAKLRGIVDSKYFNRGIMMAILVNTVSMGIEHHEQPEELTNILEICNVVFTSMFALEMILKLAAFGLFDYLRNPYNIFDSIIVIISIWEIVGQADGGLSVLRTFRLLRVLKLVRFMPALRRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHIFGCKFSLRTDTGDTVPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMASTTPWASLYFVALMTFGNYVLFNLLVAILVEGFQAEGDANRSYSDEDQSSSNLEELDKLPEGLDSSRDLKLCPIPMTPNGHLDPSLPLGGHLGPAGAMGAAPRLSLQPDPVLVALESRKSSVMSLGRMSYDQRSLSSSRSSYYGPWGRSGTWASRRSSWNSLKHKPPSAEHESLLSGERGGSCVRACEGAREDAPPRAAPLHAPHTHHAHHGPHLAHRHRHHRRTLSLDTRDSVDLAELVPVVGAHSRAAWRAAGQAPGHEDCNGRMPNIAKDVFTKMDDRRDRGEDEEEIDYTLCFRVRKMIDVYKPDWCEVREDWSVYLFSPENKFRILCQTIIAHKLFDYVVLAFIFLNCITIALERPQIEAGSTERIFLTVSNYIFTAIFVGEMTLKVVSLGLYFGEQAYLRSSWNVLDGFLVFVSIIDIVVSVASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLKPIGNIVLICCAFFIIFGILGVQLFKGKFYHCLGVDTRNITNRSDCVAANYRWVHHKYNFDNLGQALMSLFVLASKDGWVNIMYNGLDAVAVDQQPVTNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEAEEARRREEKRLRRLEKKRRKAQRLPYYATYCPTRLLIHSMCTSHYLDIFITFIICLNVVTMSLEHYNQPTSLETALKYCNYMFTTVFVLEAVLKLVAFGLRRFFKDRWNQLDLAIVLLSVMGITLEEIEINAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGKLVCNDENPCEGMSRHATFENFGMAFLTLFQVSTGDNWNGIMKDTLRDCTHDERSCLSSLQFVSPLYFVSFVLTAQFVLINVVVAVLMKHLDDSNKEAQEDAEMDAEIELEMAHGLGPGPGPCPCPCPCPCPCPCPGPRMPTSSPGAPGRGSGGAGVGGDTESHLCRHCYSPAQETLWLDSVSLIIKDSLEGELTIIDNLSGSIFHHYSSPAGCDKCHHDKQEGPVAQRSRPRLPHPSPRVTRGHHLPPQVQLAETEAFSLNSDRSSSVLLGDDLSLEDPTACPQGPKESKGELEPPEPMQAGDLDECFFPFAGEPVSAGPESLLCEMGAIPFNPVQSWLKHESNQAPPSPFSPDGSSPLLQMPAEFFHPAVSASQKGQEPGMSSGTLPKIALQGSWASLRSPSVNCTLLRQATVSDTSLDASPSSSAGSLQTTLEDSLTLSDSPRRALGPPVQVPGPRASLSPATRRRLSLRGRGLFSLRGLRAHQRSHSSGGSTSPGCTYHDSMDPSDEEGRGGAGGGGAGSEHSETLSSLSLTSLFCLPPTLPPPGLTPARKFSSTSSLAAGPGRPGATVSVRGLARSPSWAADRSKDPPGQAQLASGFGSSAPEPQPPPGESTDAASKRKR

Target ORF information:

RefSeq Version	XM_006520949.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, alpha 1I subunit (Cacna1i), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006520949.1

ORF Insert Sequence:

ATGGCTGACA GCAACTTACC ACCCTCATCT TCAGCAGCCC CGGACCCTGA GCCGGGAATC 
ACTGAGCAGC CGGGGCCCCG GAGTCCCCCT CCATCCCCTC CAGGCCTGGA GGAGCCATTG 
GATGGAACCA ACCCTGACGT CCCGCATCCA GACCTGGCAC CCGTTGCCTT CTTCTGCCTG 
CGCCAGACCA CGAGCCCACG GAACTGGTGC ATCAAGATGG TATGTAACCC GTGGTTCGAG 
TGTGTGAGCA TGCTGGTCAT CCTGCTGAAC TGTGTGACCC TGGGCATGTA CCAGCCATGT 
GACGATATGG AGTGCCTCTC AGACCGCTGC AAGATCCTGC AGGTCTTTGA TGACTTCATC 
TTCATCTTCT TCGCCATGGA GATGGTGCTT AAGATGGTGG CCCTGGGCAT TTTTGGCAAG 
AAGTGCTACC TCGGGGACAC ATGGAACCGC TTGGACTTCT TCATTGTCAT GGCAGGGATG 
GTCGAGTACT CTCTGGACCT ACAGAACATC AACCTGTCAG CCATCCGCAC TGTGCGTGTC 
CTGAGGCCTC TCAAAGCCAT CAACCGTGTT CCCAGCATGC GGATTCTGGT GAACCTGCTG 
CTGGACACGC TGCCCATGCT GGGAAACGTG CTCCTGCTCT GTTTCTTCGT CTTCTTCATC 
TTTGGCATCA TCGGCGTGCA GCTCTGGGCA GGCCTGCTGC GGAACCGCTG CTTCCTGGAG 
GAGAACTTCA CCATACAAGG GGATGTGGCC CTGCCCCCTT ACTACCAACC AGAAGAGGAC 
GACGAGATGC CCTTCATCTG CTCCCTGTCG GGGGACAATG GCATTATGGG CTGTCACGAG 
ATCCCTCCAC TCAAGGAGCA GGGCCGAGAG TGCTGCCTGT CCAAAGATGA CATGTACGAC 
TTCGGGGCGG GACGCCAAGA CCTCAACGCC AGCGGCCTCT GCGTCAACTG GAACCGCTAC 
TACAACGTCT GCCGCACGGG CAACGCCAAC CCTCACAAGG GCGCCATCAA CTTTGACAAC 
ATTGGCTATG CCTGGATCGT GATCTTTCAG GTGATCACTC TGGAAGGCTG GGTGGAGATC 
ATGTACTACG TGATGGATGC ACATTCTTTC TACAACTTCA TCTACTTCAT CCTGCTCATC 
ATAGTGGGCT CCTTCTTCAT GATCAACCTG TGCCTCGTTG TCATAGCGAC CCAGTTCTCT 
GAGACCAAGC AACGGGAGCA CCGGCTGATG CTGGAGCAAC GCCAGCGCTA CCTGTCCTCC 
AGCACGGTGG CCAGTTACGC CGAGCCCGGC GACTGCTATG AGGAGATCTT CCAATATGTC 
TGTCACATCC TTCGCAAGGC CAAGCGCCGT GCTCTAGGCC TCTACCAGGC CCTGCAGAAC 
CGGCGCCAGG CCACGGGCCC CGGGACACCA GCCCCTGCCA AGCCTGGGCC CCATGCCAAG 
GAGCCCAGCC ACTGCAAGCT GTGCCCACGA CACAGTCCCC TGGACACCAC TCCCCACACA 
CTGGTGCAGC CCATCTCTGC CATTCTGGCC TCCGATCCCA GCAGCTGCCC TCGCTGCCAG 
CACGAGGCAG GCAGGCGGCC CTCTGGCCTG GGCAGCACTG ACTCGGGCCA GGAAGGCTCA 
GGTTCTGGCG GTTCTGCAGA GGCTGAAGCC AATGGCGATG GACCCCAAAG CAGTGAGGAT 
GGGGTCTCCT CAGGTTTGGG GAAGGAGGAG GAACAGGAGG ACGGGGCAGC CCGACTGTGT 
GGGGATGTGT GGCGAGAGAC ACGGGCCAAG CTGCGGGGCA TTGTGGACAG CAAGTACTTC 
AACAGAGGCA TCATGATGGC TATTCTGGTT AACACTGTCA GCATGGGCAT CGAGCACCAT 
GAACAGCCAG AGGAACTGAC CAACATCCTG GAGATCTGCA ACGTGGTCTT CACCAGCATG 
TTTGCCCTGG AGATGATTCT GAAGCTGGCT GCCTTTGGGC TCTTTGACTA CCTGCGGAAC 
CCTTACAACA TCTTCGACAG CATCATTGTC ATCATCAGCA TCTGGGAAAT CGTGGGGCAG 
GCTGACGGCG GCCTGTCCGT GCTGCGCACC TTCCGGCTGC TGCGGGTGCT GAAGCTGGTG 
CGCTTCATGC CGGCGCTGCG GCGCCAGCTC GTGGTGCTCA TGAAGACCAT GGACAACGTG 
GCCACCTTCT GCATGCTGCT CATGCTCTTC ATTTTCATCT TCAGCATCCT TGGGATGCAT 
ATCTTTGGCT GCAAATTCAG CCTCCGCACG GACACTGGAG ACACAGTCCC TGACAGGAAG 
AACTTCGATT CCTTACTGTG GGCCATCGTC ACCGTGTTTC AGATCCTCAC TCAGGAGGAC 
TGGAACGTTG TCCTGTACAA TGGCATGGCC TCCACCACCC CCTGGGCCTC CCTCTATTTT 
GTTGCCCTCA TGACCTTTGG CAACTATGTT CTCTTCAATC TCCTGGTGGC TATCCTGGTC 
GAGGGCTTCC AGGCTGAGGG TGATGCTAAT CGCTCCTACT CAGACGAGGA CCAGAGCTCA 
TCCAACTTGG AGGAGCTTGA TAAGCTCCCA GAGGGCCTGG ACAGCAGCAG AGATCTCAAG 
CTCTGCCCAA TACCCATGAC ACCCAATGGA CACCTGGACC CTAGTCTCCC TCTGGGTGGA 
CATCTGGGTC CTGCTGGTGC CATGGGTGCT GCCCCCCGCC TCTCACTGCA GCCAGACCCA 
GTGCTGGTGG CCCTAGAATC CCGGAAAAGC AGTGTCATGT CACTGGGCAG GATGAGCTAT 
GATCAGCGAT CTTTGTCCAG CTCCCGGAGC TCCTACTATG GGCCCTGGGG CCGCAGCGGG 
ACCTGGGCCA GCCGCCGCTC CAGCTGGAAC AGCCTGAAAC ACAAGCCACC CTCAGCTGAG 
CACGAGTCCC TGCTCTCCGG GGAGCGTGGT GGTAGTTGCG TCAGGGCCTG TGAAGGCGCC 
CGGGAGGATG CGCCACCTCG CGCTGCACCC CTGCATGCCC CGCACACCCA CCACGCGCAT 
CATGGACCCC ACTTGGCACA CCGTCACCGC CACCACCGTC GGACACTGTC CCTTGATACC 
AGGGACTCTG TTGACCTGGC AGAGCTGGTG CCCGTGGTGG GTGCCCACTC GCGGGCCGCT 
TGGAGGGCGG CAGGCCAGGC CCCTGGGCAC GAGGACTGCA ATGGCAGAAT GCCCAACATA 
GCCAAGGATG TCTTCACCAA GATGGACGAC CGCCGCGACC GTGGGGAGGA CGAGGAGGAG 
ATCGACTACA CCCTGTGTTT CCGCGTCCGC AAGATGATTG ATGTGTACAA GCCTGACTGG 
TGCGAAGTCC GCGAGGACTG GTCGGTCTAC CTCTTCTCCC CTGAGAACAA GTTCCGGATC 
CTGTGTCAGA CCATTATTGC TCACAAGCTT TTTGACTACG TGGTCTTGGC CTTCATCTTC 
CTCAACTGTA TCACCATTGC TCTGGAGAGG CCCCAGATCG AAGCTGGTAG CACTGAGCGC 
ATCTTCCTCA CGGTGTCTAA CTACATCTTC ACAGCCATCT TTGTGGGCGA GATGACACTG 
AAGGTGGTCT CTTTGGGCCT ATACTTCGGT GAGCAAGCAT ACTTGCGTAG CAGCTGGAAC 
GTCCTGGATG GTTTCCTGGT CTTTGTGTCT ATCATCGATA TCGTGGTATC CGTGGCCTCT 
GCAGGGGGAG CCAAGATCCT GGGGGTCCTC CGGGTCCTGC GGCTCCTGCG TACCTTACGT 
CCTTTGAGGG TCATCAGCCG GGCCCCTGGG CTGAAGCTGG TGGTAGAGAC GCTCATCTCC 
TCCCTCAAGC CCATTGGAAA CATTGTCCTC ATCTGCTGCG CCTTCTTCAT CATCTTCGGT 
ATCCTGGGGG TGCAGCTTTT CAAAGGCAAG TTCTATCACT GTCTGGGAGT GGACACCCGA 
AACATCACCA ACCGATCAGA CTGTGTGGCC GCCAACTACC GCTGGGTGCA TCACAAATAC 
AACTTTGATA ACCTGGGCCA GGCTTTGATG TCCCTCTTTG TCTTGGCCTC CAAGGATGGC 
TGGGTGAACA TCATGTATAA TGGATTGGAT GCTGTTGCTG TGGACCAGCA GCCGGTGACA 
AACCACAACC CCTGGATGCT GCTGTACTTC ATCTCCTTCC TGCTCATCGT CAGCTTCTTC 
GTGCTCAACA TGTTCGTGGG AGTGGTCGTG GAGAACTTCC ACAAGTGCCG GCAGCATCAG 
GAGGCTGAGG AGGCACGGCG GCGTGAGGAG AAACGGCTAC GGCGCCTGGA AAAGAAGCGC 
CGTAAGGCTC AGAGGCTGCC CTACTATGCC ACCTACTGTC CCACAAGACT GCTCATCCAC 
TCCATGTGCA CTAGCCACTA CCTGGACATC TTCATCACCT TCATCATCTG CCTCAACGTG 
GTCACCATGT CCCTGGAGCA CTACAACCAG CCTACATCCC TAGAGACAGC CCTCAAGTAC 
TGCAACTACA TGTTCACCAC TGTCTTTGTG CTGGAGGCTG TGCTGAAGCT GGTGGCGTTT 
GGCCTGAGGC GTTTCTTCAA GGACCGATGG AACCAGCTGG ACTTGGCCAT TGTGCTGCTG 
TCTGTCATGG GCATCACACT GGAGGAGATT GAGATCAATG CGGCCCTGCC CATCAACCCC 
ACCATCATCC GTATCATGCG TGTTCTGCGT ATTGCCCGGG TGTTGAAGCT GTTGAAGATG 
GCCACAGGAA TGCGGGCCCT GCTGGACACA GTGGTACAGG CTCTGCCCCA GGTGGGCAAC 
CTGGGCCTCC TCTTCATGCT GCTCTTCTTC ATCTACGCCG CTCTGGGAGT GGAGCTCTTC 
GGAAAGCTGG TCTGCAATGA CGAGAACCCG TGTGAGGGCA TGAGCCGGCA CGCCACCTTC 
GAGAACTTCG GCATGGCCTT CCTCACACTC TTCCAGGTCT CCACAGGCGA TAACTGGAAT 
GGGATTATGA AGGACACTCT GCGAGACTGC ACCCACGACG AGCGCAGCTG CCTGAGCAGC 
CTGCAGTTTG TGTCGCCTCT CTACTTTGTG AGCTTCGTAC TCACAGCGCA GTTTGTGCTC 
ATCAACGTGG TGGTGGCCGT GCTGATGAAA CATCTGGATG ACAGCAACAA GGAGGCCCAG 
GAGGATGCAG AGATGGATGC TGAGATCGAG CTGGAGATGG CCCATGGCCT AGGCCCCGGG 
CCCGGCCCCT GTCCCTGTCC CTGTCCCTGC CCCTGCCCCT GTCCCTGTCC TGGCCCGAGG 
ATGCCCACCA GTTCACCGGG GGCTCCAGGC CGAGGATCAG GAGGGGCAGG CGTTGGAGGC 
GACACTGAGA GCCACCTGTG CCGGCACTGC TATTCTCCAG CCCAGGAGAC CCTGTGGCTG 
GACAGCGTCT CTTTAATCAT CAAGGACTCC TTGGAGGGGG AGCTGACCAT CATTGACAAC 
CTGTCTGGCT CCATCTTCCA CCACTACTCC TCACCTGCCG GCTGTGACAA GTGTCACCAT 
GACAAGCAAG AGGGACCTGT TGCTCAGCGC TCCAGGCCCA GGCTGCCCCA TCCCAGCCCC 
AGGGTGACCC GAGGCCACCA CCTCCCCCCG CAGGTGCAGC TGGCTGAGAC AGAGGCCTTC 
TCCCTGAACT CAGACAGGTC CTCGTCCGTC CTGCTGGGGG ATGACCTGAG TCTTGAGGAC 
CCCACGGCCT GCCCGCAGGG CCCCAAGGAG AGCAAGGGTG AGCTAGAGCC TCCGGAGCCC 
ATGCAGGCTG GAGACCTGGA TGAATGCTTC TTCCCCTTTG CCGGTGAGCC AGTGTCTGCA 
GGCCCAGAGA GCCTGCTGTG CGAGATGGGG GCCATTCCAT TCAACCCCGT CCAGTCCTGG 
CTCAAACATG AGAGCAACCA AGCACCCCCA AGCCCTTTCT CCCCGGATGG TTCCAGCCCT 
CTGCTGCAGA TGCCTGCTGA GTTCTTCCAC CCTGCTGTGT CTGCCAGCCA GAAGGGGCAG 
GAACCGGGCA TGAGTTCAGG GACCCTGCCC AAGATTGCAC TTCAGGGGTC CTGGGCATCC 
CTGAGGTCAC CGAGTGTCAA CTGCACCCTC TTGCGCCAGG CTACTGTGAG TGACACGTCT 
TTGGATGCCA GCCCTAGCAG CTCAGCGGGC AGCCTGCAGA CCACACTGGA GGACAGTCTG 
ACCCTGAGTG ACAGTCCTCG GCGTGCCCTG GGACCACCAG TCCAGGTGCC AGGGCCACGG 
GCTAGCCTGT CACCTGCCAC CCGGCGCCGC CTCAGCCTGC GGGGGCGTGG CCTGTTTAGT 
CTGCGTGGGC TGCGGGCCCA TCAGCGTAGC CACAGCAGCG GTGGCTCCAC CAGCCCTGGC 
TGCACTTACC ACGACTCCAT GGACCCCTCT GATGAGGAGG GCCGCGGGGG CGCAGGTGGT 
GGGGGCGCGG GCAGTGAGCA CTCCGAGACC CTCAGCAGCC TGTCACTCAC ATCTCTCTTC 
TGCCTACCAC CCACGCTGCC ACCACCCGGT CTCACCCCAG CCAGGAAGTT CAGCAGTACC 
AGCAGCCTGG CTGCTGGACC TGGCCGTCCT GGCGCCACTG TCTCGGTCCG CGGCCTGGCC 
AGGAGTCCCT CATGGGCTGC AGACCGCAGC AAGGACCCAC CAGGCCAGGC ACAGCTGGCC 
TCCGGCTTCG GCTCCTCAGC ACCCGAGCCA CAGCCACCTC CCGGGGAGTC AACGGATGCG 
GCGAGCAAAA GGAAGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1i ( XM_006520950.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006521013.1 Mus musculus calcium channel, voltage-dependent, alpha 1I subunit (Cacna1i), transcript variant X2, mRNA.

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CloneID	OMu51300
Clone ID Related Accession (Same CDS sequence)	XM_006520950.2 , XM_006520950.1
Accession Version	XM_006520950.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent T-type calcium channel subunit alpha-1I isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGACA GCAACTTACC ACCCTCATCT TCAGCAGCCC CGGACCCTGA GCCGGGAATC 
ACTGAGCAGC CGGGGCCCCG GAGTCCCCCT CCATCCCCTC CAGGCCTGGA GGAGCCATTG 
GATGGAACCA ACCCTGACGT CCCGCATCCA GACCTGGCAC CCGTTGCCTT CTTCTGCCTG 
CGCCAGACCA CGAGCCCACG GAACTGGTGC ATCAAGATGG TATGTAACCC GTGGTTCGAG 
TGTGTGAGCA TGCTGGTCAT CCTGCTGAAC TGTGTGACCC TGGGCATGTA CCAGCCATGT 
GACGATATGG AGTGCCTCTC AGACCGCTGC AAGATCCTGC AGGTCTTTGA TGACTTCATC 
TTCATCTTCT TCGCCATGGA GATGGTGCTT AAGATGGTGG CCCTGGGCAT TTTTGGCAAG 
AAGTGCTACC TCGGGGACAC ATGGAACCGC TTGGACTTCT TCATTGTCAT GGCAGGGATG 
GTCGAGTACT CTCTGGACCT ACAGAACATC AACCTGTCAG CCATCCGCAC TGTGCGTGTC 
CTGAGGCCTC TCAAAGCCAT CAACCGTGTT CCCAGCATGC GGATTCTGGT GAACCTGCTG 
CTGGACACGC TGCCCATGCT GGGAAACGTG CTCCTGCTCT GTTTCTTCGT CTTCTTCATC 
TTTGGCATCA TCGGCGTGCA GCTCTGGGCA GGCCTGCTGC GGAACCGCTG CTTCCTGGAG 
GAGAACTTCA CCATACAAGG GGATGTGGCC CTGCCCCCTT ACTACCAACC AGAAGAGGAC 
GACGAGATGC CCTTCATCTG CTCCCTGTCG GGGGACAATG GCATTATGGG CTGTCACGAG 
ATCCCTCCAC TCAAGGAGCA GGGCCGAGAG TGCTGCCTGT CCAAAGATGA CATGTACGAC 
TTCGGGGCGG GACGCCAAGA CCTCAACGCC AGCGGCCTCT GCGTCAACTG GAACCGCTAC 
TACAACGTCT GCCGCACGGG CAACGCCAAC CCTCACAAGG GCGCCATCAA CTTTGACAAC 
ATTGGCTATG CCTGGATCGT GATCTTTCAG GTGATCACTC TGGAAGGCTG GGTGGAGATC 
ATGTACTACG TGATGGATGC ACATTCTTTC TACAACTTCA TCTACTTCAT CCTGCTCATC 
ATAGTGGGCT CCTTCTTCAT GATCAACCTG TGCCTCGTTG TCATAGCGAC CCAGTTCTCT 
GAGACCAAGC AACGGGAGCA CCGGCTGATG CTGGAGCAAC GCCAGCGCTA CCTGTCCTCC 
AGCACGGTGG CCAGTTACGC CGAGCCCGGC GACTGCTATG AGGAGATCTT CCAATATGTC 
TGTCACATCC TTCGCAAGGC CAAGCGCCGT GCTCTAGGCC TCTACCAGGC CCTGCAGAAC 
CGGCGCCAGG CCACGGGCCC CGGGACACCA GCCCCTGCCA AGCCTGGGCC CCATGCCAAG 
GAGCCCAGCC ACTGCAAGCT GTGCCCACGA CACAGTCCCC TGGACACCAC TCCCCACACA 
CTGGTGCAGC CCATCTCTGC CATTCTGGCC TCCGATCCCA GCAGCTGCCC TCGCTGCCAG 
CACGAGGCAG GCAGGCGGCC CTCTGGCCTG GGCAGCACTG ACTCGGGCCA GGAAGGCTCA 
GGTTCTGGCG GTTCTGCAGA GGCTGAAGCC AATGGCGATG GACCCCAAAG CAGTGAGGAT 
GGGGTCTCCT CAGGTTTGGG GAAGGAGGAG GAACAGGAGG ACGGGGCAGC CCGACTGTGT 
GGGGATGTGT GGCGAGAGAC ACGGGCCAAG CTGCGGGGCA TTGTGGACAG CAAGTACTTC 
AACAGAGGCA TCATGATGGC TATTCTGGTT AACACTGTCA GCATGGGCAT CGAGCACCAT 
GAACAGCCAG AGGAACTGAC CAACATCCTG GAGATCTGCA ACGTGGTCTT CACCAGCATG 
TTTGCCCTGG AGATGATTCT GAAGCTGGCT GCCTTTGGGC TCTTTGACTA CCTGCGGAAC 
CCTTACAACA TCTTCGACAG CATCATTGTC ATCATCAGCA TCTGGGAAAT CGTGGGGCAG 
GCTGACGGCG GCCTGTCCGT GCTGCGCACC TTCCGGCTGC TGCGGGTGCT GAAGCTGGTG 
CGCTTCATGC CGGCGCTGCG GCGCCAGCTC GTGGTGCTCA TGAAGACCAT GGACAACGTG 
GCCACCTTCT GCATGCTGCT CATGCTCTTC ATTTTCATCT TCAGCATCCT TGGGATGCAT 
ATCTTTGGCT GCAAATTCAG CCTCCGCACG GACACTGGAG ACACAGTCCC TGACAGGAAG 
AACTTCGATT CCTTACTGTG GGCCATCGTC ACCGTGTTTC AGATCCTCAC TCAGGAGGAC 
TGGAACGTTG TCCTGTACAA TGGCATGGCC TCCACCACCC CCTGGGCCTC CCTCTATTTT 
GTTGCCCTCA TGACCTTTGG CAACTATGTT CTCTTCAATC TCCTGGTGGC TATCCTGGTC 
GAGGGCTTCC AGGCTGAGGG TGATGCTAAT CGCTCCTACT CAGACGAGGA CCAGAGCTCA 
TCCAACTTGG AGGAGCTTGA TAAGCTCCCA GAGGGCCTGG ACAGCAGCAG AGATCTCAAG 
CTCTGCCCAA TACCCATGAC ACCCAATGGA CACCTGGACC CTAGTCTCCC TCTGGGTGGA 
CATCTGGGTC CTGCTGGTGC CATGGGTGCT GCCCCCCGCC TCTCACTGCA GCCAGACCCA 
GTGCTGGTGG CCCTAGAATC CCGGAAAAGC AGTGTCATGT CACTGGGCAG GATGAGCTAT 
GATCAGCGAT CTTTGTCCAG CTCCCGGAGC TCCTACTATG GGCCCTGGGG CCGCAGCGGG 
ACCTGGGCCA GCCGCCGCTC CAGCTGGAAC AGCCTGAAAC ACAAGCCACC CTCAGCTGAG 
CACGAGTCCC TGCTCTCCGG GGAGCGTGGT GGTAGTTGCG TCAGGGCCTG TGAAGGCGCC 
CGGGAGGATG CGCCACCTCG CGCTGCACCC CTGCATGCCC CGCACACCCA CCACGCGCAT 
CATGGACCCC ACTTGGCACA CCGTCACCGC CACCACCGTC GGACACTGTC CCTTGATACC 
AGGGACTCTG TTGACCTGGC AGAGCTGGTG CCCGTGGTGG GTGCCCACTC GCGGGCCGCT 
TGGAGGGCGG CAGGCCAGGC CCCTGGGCAC GAGGACTGCA ATGGCAGAAT GCCCAACATA 
GCCAAGGATG TCTTCACCAA GATGGACGAC CGCCGCGACC GTGGGGAGGA CGAGGAGGAG 
ATCGACTACA CCCTGTGTTT CCGCGTCCGC AAGATGATTG ATGTGTACAA GCCTGACTGG 
TGCGAAGTCC GCGAGGACTG GTCGGTCTAC CTCTTCTCCC CTGAGAACAA GTTCCGGATC 
CTGTGTCAGA CCATTATTGC TCACAAGCTT TTTGACTACG TGGTCTTGGC CTTCATCTTC 
CTCAACTGTA TCACCATTGC TCTGGAGAGG CCCCAGATCG AAGCTGGTAG CACTGAGCGC 
ATCTTCCTCA CGGTGTCTAA CTACATCTTC ACAGCCATCT TTGTGGGCGA GATGACACTG 
AAGGTGGTCT CTTTGGGCCT ATACTTCGGT GAGCAAGCAT ACTTGCGTAG CAGCTGGAAC 
GTCCTGGATG GTTTCCTGGT CTTTGTGTCT ATCATCGATA TCGTGGTATC CGTGGCCTCT 
GCAGGGGGAG CCAAGATCCT GGGGGTCCTC CGGGTCCTGC GGCTCCTGCG TACCTTACGT 
CCTTTGAGGG TCATCAGCCG GGCCCCTGGG CTGAAGCTGG TGGTAGAGAC GCTCATCTCC 
TCCCTCAAGC CCATTGGAAA CATTGTCCTC ATCTGCTGCG CCTTCTTCAT CATCTTCGGT 
ATCCTGGGGG TGCAGCTTTT CAAAGGCAAG TTCTATCACT GTCTGGGAGT GGACACCCGA 
AACATCACCA ACCGATCAGA CTGTGTGGCC GCCAACTACC GCTGGGTGCA TCACAAATAC 
AACTTTGATA ACCTGGGCCA GGCTTTGATG TCCCTCTTTG TCTTGGCCTC CAAGGATGGC 
TGGGTGAACA TCATGTATAA TGGATTGGAT GCTGTTGCTG TGGACCAGCA GCCGGTGACA 
AACCACAACC CCTGGATGCT GCTGTACTTC ATCTCCTTCC TGCTCATCGT CAGCTTCTTC 
GTGCTCAACA TGTTCGTGGG AGTGGTCGTG GAGAACTTCC ACAAGTGCCG GCAGCATCAG 
GAGGCTGAGG AGGCACGGCG GCGTGAGGAG AAACGGCTAC GGCGCCTGGA AAAGAAGCGC 
CGTAAGGCTC AGAGGCTGCC CTACTATGCC ACCTACTGTC CCACAAGACT GCTCATCCAC 
TCCATGTGCA CTAGCCACTA CCTGGACATC TTCATCACCT TCATCATCTG CCTCAACGTG 
GTCACCATGT CCCTGGAGCA CTACAACCAG CCTACATCCC TAGAGACAGC CCTCAAGTAC 
TGCAACTACA TGTTCACCAC TGTCTTTGTG CTGGAGGCTG TGCTGAAGCT GGTGGCGTTT 
GGCCTGAGGC GTTTCTTCAA GGACCGATGG AACCAGCTGG ACTTGGCCAT TGTGCTGCTG 
TCTGTCATGG GCATCACACT GGAGGAGATT GAGATCAATG CGGCCCTGCC CATCAACCCC 
ACCATCATCC GTATCATGCG TGTTCTGCGT ATTGCCCGGG TGTTGAAGCT GTTGAAGATG 
GCCACAGGAA TGCGGGCCCT GCTGGACACA GTGGTACAGG CTCTGCCCCA GGTGGGCAAC 
CTGGGCCTCC TCTTCATGCT GCTCTTCTTC ATCTACGCCG CTCTGGGAGT GGAGCTCTTC 
GGAAAGCTGG TCTGCAATGA CGAGAACCCG TGTGAGGGCA TGAGCCGGCA CGCCACCTTC 
GAGAACTTCG GCATGGCCTT CCTCACACTC TTCCAGGTCT CCACAGGCGA TAACTGGAAT 
GGGATTATGA AGGACACTCT GCGAGACTGC ACCCACGACG AGCGCAGCTG CCTGAGCAGC 
CTGCAGTTTG TGTCGCCTCT CTACTTTGTG AGCTTCGTAC TCACAGCGCA GTTTGTGCTC 
ATCAACGTGG TGGTGGCCGT GCTGATGAAA CATCTGGATG ACAGCAACAA GGAGGCCCAG 
GAGGATGCAG AGATGGATGC TGAGATCGAG CTGGAGATGG CCCATGGCCT AGGCCCCGGG 
CCCGGCCCCT GTCCCTGTCC CTGTCCCTGC CCCTGCCCCT GTCCCTGTCC TGGCCCGAGG 
ATGCCCACCA GTTCACCGGG GGCTCCAGGC CGAGGATCAG GAGGGGCAGG CGTTGGAGGC 
GACACTGAGA GCCACCTGTG CCGGCACTGC TATTCTCCAG CCCAGGACTC CTTGGAGGGG 
GAGCTGACCA TCATTGACAA CCTGTCTGGC TCCATCTTCC ACCACTACTC CTCACCTGCC 
GGCTGTGACA AGTGTCACCA TGACAAGCAA GAGGGACCTG TTGCTCAGCG CTCCAGGCCC 
AGGCTGCCCC ATCCCAGCCC CAGGGTGACC CGAGGCCACC ACCTCCCCCC GCAGGTGCAG 
CTGGCTGAGA CAGAGGCCTT CTCCCTGAAC TCAGACAGGT CCTCGTCCGT CCTGCTGGGG 
GATGACCTGA GTCTTGAGGA CCCCACGGCC TGCCCGCAGG GCCCCAAGGA GAGCAAGGGT 
GAGCTAGAGC CTCCGGAGCC CATGCAGGCT GGAGACCTGG ATGAATGCTT CTTCCCCTTT 
GCCGGTGAGC CAGTGTCTGC AGGCCCAGAG AGCCTGCTGT GCGAGATGGG GGCCATTCCA 
TTCAACCCCG TCCAGTCCTG GCTCAAACAT GAGAGCAACC AAGCACCCCC AAGCCCTTTC 
TCCCCGGATG GTTCCAGCCC TCTGCTGCAG ATGCCTGCTG AGTTCTTCCA CCCTGCTGTG 
TCTGCCAGCC AGAAGGGGCA GGAACCGGGC ATGAGTTCAG GGACCCTGCC CAAGATTGCA 
CTTCAGGGGT CCTGGGCATC CCTGAGGTCA CCGAGTGTCA ACTGCACCCT CTTGCGCCAG 
GCTACTGTGA GTGACACGTC TTTGGATGCC AGCCCTAGCA GCTCAGCGGG CAGCCTGCAG 
ACCACACTGG AGGACAGTCT GACCCTGAGT GACAGTCCTC GGCGTGCCCT GGGACCACCA 
GTCCAGGTGC CAGGGCCACG GGCTAGCCTG TCACCTGCCA CCCGGCGCCG CCTCAGCCTG 
CGGGGGCGTG GCCTGTTTAG TCTGCGTGGG CTGCGGGCCC ATCAGCGTAG CCACAGCAGC 
GGTGGCTCCA CCAGCCCTGG CTGCACTTAC CACGACTCCA TGGACCCCTC TGATGAGGAG 
GGCCGCGGGG GCGCAGGTGG TGGGGGCGCG GGCAGTGAGC ACTCCGAGAC CCTCAGCAGC 
CTGTCACTCA CATCTCTCTT CTGCCTACCA CCCACGCTGC CACCACCCGG TCTCACCCCA 
GCCAGGAAGT TCAGCAGTAC CAGCAGCCTG GCTGCTGGAC CTGGCCGTCC TGGCGCCACT 
GTCTCGGTCC GCGGCCTGGC CAGGAGTCCC TCATGGGCTG CAGACCGCAG CAAGGACCCA 
CCAGGCCAGG CACAGCTGGC CTCCGGCTTC GGCTCCTCAG CACCCGAGCC ACAGCCACCT 
CCCGGGGAGT CAACGGATGC GGCGAGCAAA AGGAAGAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006521013.1
CDS	234..6875
Translation	MADSNLPPSSSAAPDPEPGITEQPGPRSPPPSPPGLEEPLDGTNPDVPHPDLAPVAFFCLRQTTSPRNWCIKMVCNPWFECVSMLVILLNCVTLGMYQPCDDMECLSDRCKILQVFDDFIFIFFAMEMVLKMVALGIFGKKCYLGDTWNRLDFFIVMAGMVEYSLDLQNINLSAIRTVRVLRPLKAINRVPSMRILVNLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLWAGLLRNRCFLEENFTIQGDVALPPYYQPEEDDEMPFICSLSGDNGIMGCHEIPPLKEQGRECCLSKDDMYDFGAGRQDLNASGLCVNWNRYYNVCRTGNANPHKGAINFDNIGYAWIVIFQVITLEGWVEIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQREHRLMLEQRQRYLSSSTVASYAEPGDCYEEIFQYVCHILRKAKRRALGLYQALQNRRQATGPGTPAPAKPGPHAKEPSHCKLCPRHSPLDTTPHTLVQPISAILASDPSSCPRCQHEAGRRPSGLGSTDSGQEGSGSGGSAEAEANGDGPQSSEDGVSSGLGKEEEQEDGAARLCGDVWRETRAKLRGIVDSKYFNRGIMMAILVNTVSMGIEHHEQPEELTNILEICNVVFTSMFALEMILKLAAFGLFDYLRNPYNIFDSIIVIISIWEIVGQADGGLSVLRTFRLLRVLKLVRFMPALRRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHIFGCKFSLRTDTGDTVPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMASTTPWASLYFVALMTFGNYVLFNLLVAILVEGFQAEGDANRSYSDEDQSSSNLEELDKLPEGLDSSRDLKLCPIPMTPNGHLDPSLPLGGHLGPAGAMGAAPRLSLQPDPVLVALESRKSSVMSLGRMSYDQRSLSSSRSSYYGPWGRSGTWASRRSSWNSLKHKPPSAEHESLLSGERGGSCVRACEGAREDAPPRAAPLHAPHTHHAHHGPHLAHRHRHHRRTLSLDTRDSVDLAELVPVVGAHSRAAWRAAGQAPGHEDCNGRMPNIAKDVFTKMDDRRDRGEDEEEIDYTLCFRVRKMIDVYKPDWCEVREDWSVYLFSPENKFRILCQTIIAHKLFDYVVLAFIFLNCITIALERPQIEAGSTERIFLTVSNYIFTAIFVGEMTLKVVSLGLYFGEQAYLRSSWNVLDGFLVFVSIIDIVVSVASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLKPIGNIVLICCAFFIIFGILGVQLFKGKFYHCLGVDTRNITNRSDCVAANYRWVHHKYNFDNLGQALMSLFVLASKDGWVNIMYNGLDAVAVDQQPVTNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEAEEARRREEKRLRRLEKKRRKAQRLPYYATYCPTRLLIHSMCTSHYLDIFITFIICLNVVTMSLEHYNQPTSLETALKYCNYMFTTVFVLEAVLKLVAFGLRRFFKDRWNQLDLAIVLLSVMGITLEEIEINAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGKLVCNDENPCEGMSRHATFENFGMAFLTLFQVSTGDNWNGIMKDTLRDCTHDERSCLSSLQFVSPLYFVSFVLTAQFVLINVVVAVLMKHLDDSNKEAQEDAEMDAEIELEMAHGLGPGPGPCPCPCPCPCPCPCPGPRMPTSSPGAPGRGSGGAGVGGDTESHLCRHCYSPAQDSLEGELTIIDNLSGSIFHHYSSPAGCDKCHHDKQEGPVAQRSRPRLPHPSPRVTRGHHLPPQVQLAETEAFSLNSDRSSSVLLGDDLSLEDPTACPQGPKESKGELEPPEPMQAGDLDECFFPFAGEPVSAGPESLLCEMGAIPFNPVQSWLKHESNQAPPSPFSPDGSSPLLQMPAEFFHPAVSASQKGQEPGMSSGTLPKIALQGSWASLRSPSVNCTLLRQATVSDTSLDASPSSSAGSLQTTLEDSLTLSDSPRRALGPPVQVPGPRASLSPATRRRLSLRGRGLFSLRGLRAHQRSHSSGGSTSPGCTYHDSMDPSDEEGRGGAGGGGAGSEHSETLSSLSLTSLFCLPPTLPPPGLTPARKFSSTSSLAAGPGRPGATVSVRGLARSPSWAADRSKDPPGQAQLASGFGSSAPEPQPPPGESTDAASKRKR

Target ORF information:

RefSeq Version	XM_006520950.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, alpha 1I subunit (Cacna1i), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006520950.1

ORF Insert Sequence:

ATGGCTGACA GCAACTTACC ACCCTCATCT TCAGCAGCCC CGGACCCTGA GCCGGGAATC 
ACTGAGCAGC CGGGGCCCCG GAGTCCCCCT CCATCCCCTC CAGGCCTGGA GGAGCCATTG 
GATGGAACCA ACCCTGACGT CCCGCATCCA GACCTGGCAC CCGTTGCCTT CTTCTGCCTG 
CGCCAGACCA CGAGCCCACG GAACTGGTGC ATCAAGATGG TATGTAACCC GTGGTTCGAG 
TGTGTGAGCA TGCTGGTCAT CCTGCTGAAC TGTGTGACCC TGGGCATGTA CCAGCCATGT 
GACGATATGG AGTGCCTCTC AGACCGCTGC AAGATCCTGC AGGTCTTTGA TGACTTCATC 
TTCATCTTCT TCGCCATGGA GATGGTGCTT AAGATGGTGG CCCTGGGCAT TTTTGGCAAG 
AAGTGCTACC TCGGGGACAC ATGGAACCGC TTGGACTTCT TCATTGTCAT GGCAGGGATG 
GTCGAGTACT CTCTGGACCT ACAGAACATC AACCTGTCAG CCATCCGCAC TGTGCGTGTC 
CTGAGGCCTC TCAAAGCCAT CAACCGTGTT CCCAGCATGC GGATTCTGGT GAACCTGCTG 
CTGGACACGC TGCCCATGCT GGGAAACGTG CTCCTGCTCT GTTTCTTCGT CTTCTTCATC 
TTTGGCATCA TCGGCGTGCA GCTCTGGGCA GGCCTGCTGC GGAACCGCTG CTTCCTGGAG 
GAGAACTTCA CCATACAAGG GGATGTGGCC CTGCCCCCTT ACTACCAACC AGAAGAGGAC 
GACGAGATGC CCTTCATCTG CTCCCTGTCG GGGGACAATG GCATTATGGG CTGTCACGAG 
ATCCCTCCAC TCAAGGAGCA GGGCCGAGAG TGCTGCCTGT CCAAAGATGA CATGTACGAC 
TTCGGGGCGG GACGCCAAGA CCTCAACGCC AGCGGCCTCT GCGTCAACTG GAACCGCTAC 
TACAACGTCT GCCGCACGGG CAACGCCAAC CCTCACAAGG GCGCCATCAA CTTTGACAAC 
ATTGGCTATG CCTGGATCGT GATCTTTCAG GTGATCACTC TGGAAGGCTG GGTGGAGATC 
ATGTACTACG TGATGGATGC ACATTCTTTC TACAACTTCA TCTACTTCAT CCTGCTCATC 
ATAGTGGGCT CCTTCTTCAT GATCAACCTG TGCCTCGTTG TCATAGCGAC CCAGTTCTCT 
GAGACCAAGC AACGGGAGCA CCGGCTGATG CTGGAGCAAC GCCAGCGCTA CCTGTCCTCC 
AGCACGGTGG CCAGTTACGC CGAGCCCGGC GACTGCTATG AGGAGATCTT CCAATATGTC 
TGTCACATCC TTCGCAAGGC CAAGCGCCGT GCTCTAGGCC TCTACCAGGC CCTGCAGAAC 
CGGCGCCAGG CCACGGGCCC CGGGACACCA GCCCCTGCCA AGCCTGGGCC CCATGCCAAG 
GAGCCCAGCC ACTGCAAGCT GTGCCCACGA CACAGTCCCC TGGACACCAC TCCCCACACA 
CTGGTGCAGC CCATCTCTGC CATTCTGGCC TCCGATCCCA GCAGCTGCCC TCGCTGCCAG 
CACGAGGCAG GCAGGCGGCC CTCTGGCCTG GGCAGCACTG ACTCGGGCCA GGAAGGCTCA 
GGTTCTGGCG GTTCTGCAGA GGCTGAAGCC AATGGCGATG GACCCCAAAG CAGTGAGGAT 
GGGGTCTCCT CAGGTTTGGG GAAGGAGGAG GAACAGGAGG ACGGGGCAGC CCGACTGTGT 
GGGGATGTGT GGCGAGAGAC ACGGGCCAAG CTGCGGGGCA TTGTGGACAG CAAGTACTTC 
AACAGAGGCA TCATGATGGC TATTCTGGTT AACACTGTCA GCATGGGCAT CGAGCACCAT 
GAACAGCCAG AGGAACTGAC CAACATCCTG GAGATCTGCA ACGTGGTCTT CACCAGCATG 
TTTGCCCTGG AGATGATTCT GAAGCTGGCT GCCTTTGGGC TCTTTGACTA CCTGCGGAAC 
CCTTACAACA TCTTCGACAG CATCATTGTC ATCATCAGCA TCTGGGAAAT CGTGGGGCAG 
GCTGACGGCG GCCTGTCCGT GCTGCGCACC TTCCGGCTGC TGCGGGTGCT GAAGCTGGTG 
CGCTTCATGC CGGCGCTGCG GCGCCAGCTC GTGGTGCTCA TGAAGACCAT GGACAACGTG 
GCCACCTTCT GCATGCTGCT CATGCTCTTC ATTTTCATCT TCAGCATCCT TGGGATGCAT 
ATCTTTGGCT GCAAATTCAG CCTCCGCACG GACACTGGAG ACACAGTCCC TGACAGGAAG 
AACTTCGATT CCTTACTGTG GGCCATCGTC ACCGTGTTTC AGATCCTCAC TCAGGAGGAC 
TGGAACGTTG TCCTGTACAA TGGCATGGCC TCCACCACCC CCTGGGCCTC CCTCTATTTT 
GTTGCCCTCA TGACCTTTGG CAACTATGTT CTCTTCAATC TCCTGGTGGC TATCCTGGTC 
GAGGGCTTCC AGGCTGAGGG TGATGCTAAT CGCTCCTACT CAGACGAGGA CCAGAGCTCA 
TCCAACTTGG AGGAGCTTGA TAAGCTCCCA GAGGGCCTGG ACAGCAGCAG AGATCTCAAG 
CTCTGCCCAA TACCCATGAC ACCCAATGGA CACCTGGACC CTAGTCTCCC TCTGGGTGGA 
CATCTGGGTC CTGCTGGTGC CATGGGTGCT GCCCCCCGCC TCTCACTGCA GCCAGACCCA 
GTGCTGGTGG CCCTAGAATC CCGGAAAAGC AGTGTCATGT CACTGGGCAG GATGAGCTAT 
GATCAGCGAT CTTTGTCCAG CTCCCGGAGC TCCTACTATG GGCCCTGGGG CCGCAGCGGG 
ACCTGGGCCA GCCGCCGCTC CAGCTGGAAC AGCCTGAAAC ACAAGCCACC CTCAGCTGAG 
CACGAGTCCC TGCTCTCCGG GGAGCGTGGT GGTAGTTGCG TCAGGGCCTG TGAAGGCGCC 
CGGGAGGATG CGCCACCTCG CGCTGCACCC CTGCATGCCC CGCACACCCA CCACGCGCAT 
CATGGACCCC ACTTGGCACA CCGTCACCGC CACCACCGTC GGACACTGTC CCTTGATACC 
AGGGACTCTG TTGACCTGGC AGAGCTGGTG CCCGTGGTGG GTGCCCACTC GCGGGCCGCT 
TGGAGGGCGG CAGGCCAGGC CCCTGGGCAC GAGGACTGCA ATGGCAGAAT GCCCAACATA 
GCCAAGGATG TCTTCACCAA GATGGACGAC CGCCGCGACC GTGGGGAGGA CGAGGAGGAG 
ATCGACTACA CCCTGTGTTT CCGCGTCCGC AAGATGATTG ATGTGTACAA GCCTGACTGG 
TGCGAAGTCC GCGAGGACTG GTCGGTCTAC CTCTTCTCCC CTGAGAACAA GTTCCGGATC 
CTGTGTCAGA CCATTATTGC TCACAAGCTT TTTGACTACG TGGTCTTGGC CTTCATCTTC 
CTCAACTGTA TCACCATTGC TCTGGAGAGG CCCCAGATCG AAGCTGGTAG CACTGAGCGC 
ATCTTCCTCA CGGTGTCTAA CTACATCTTC ACAGCCATCT TTGTGGGCGA GATGACACTG 
AAGGTGGTCT CTTTGGGCCT ATACTTCGGT GAGCAAGCAT ACTTGCGTAG CAGCTGGAAC 
GTCCTGGATG GTTTCCTGGT CTTTGTGTCT ATCATCGATA TCGTGGTATC CGTGGCCTCT 
GCAGGGGGAG CCAAGATCCT GGGGGTCCTC CGGGTCCTGC GGCTCCTGCG TACCTTACGT 
CCTTTGAGGG TCATCAGCCG GGCCCCTGGG CTGAAGCTGG TGGTAGAGAC GCTCATCTCC 
TCCCTCAAGC CCATTGGAAA CATTGTCCTC ATCTGCTGCG CCTTCTTCAT CATCTTCGGT 
ATCCTGGGGG TGCAGCTTTT CAAAGGCAAG TTCTATCACT GTCTGGGAGT GGACACCCGA 
AACATCACCA ACCGATCAGA CTGTGTGGCC GCCAACTACC GCTGGGTGCA TCACAAATAC 
AACTTTGATA ACCTGGGCCA GGCTTTGATG TCCCTCTTTG TCTTGGCCTC CAAGGATGGC 
TGGGTGAACA TCATGTATAA TGGATTGGAT GCTGTTGCTG TGGACCAGCA GCCGGTGACA 
AACCACAACC CCTGGATGCT GCTGTACTTC ATCTCCTTCC TGCTCATCGT CAGCTTCTTC 
GTGCTCAACA TGTTCGTGGG AGTGGTCGTG GAGAACTTCC ACAAGTGCCG GCAGCATCAG 
GAGGCTGAGG AGGCACGGCG GCGTGAGGAG AAACGGCTAC GGCGCCTGGA AAAGAAGCGC 
CGTAAGGCTC AGAGGCTGCC CTACTATGCC ACCTACTGTC CCACAAGACT GCTCATCCAC 
TCCATGTGCA CTAGCCACTA CCTGGACATC TTCATCACCT TCATCATCTG CCTCAACGTG 
GTCACCATGT CCCTGGAGCA CTACAACCAG CCTACATCCC TAGAGACAGC CCTCAAGTAC 
TGCAACTACA TGTTCACCAC TGTCTTTGTG CTGGAGGCTG TGCTGAAGCT GGTGGCGTTT 
GGCCTGAGGC GTTTCTTCAA GGACCGATGG AACCAGCTGG ACTTGGCCAT TGTGCTGCTG 
TCTGTCATGG GCATCACACT GGAGGAGATT GAGATCAATG CGGCCCTGCC CATCAACCCC 
ACCATCATCC GTATCATGCG TGTTCTGCGT ATTGCCCGGG TGTTGAAGCT GTTGAAGATG 
GCCACAGGAA TGCGGGCCCT GCTGGACACA GTGGTACAGG CTCTGCCCCA GGTGGGCAAC 
CTGGGCCTCC TCTTCATGCT GCTCTTCTTC ATCTACGCCG CTCTGGGAGT GGAGCTCTTC 
GGAAAGCTGG TCTGCAATGA CGAGAACCCG TGTGAGGGCA TGAGCCGGCA CGCCACCTTC 
GAGAACTTCG GCATGGCCTT CCTCACACTC TTCCAGGTCT CCACAGGCGA TAACTGGAAT 
GGGATTATGA AGGACACTCT GCGAGACTGC ACCCACGACG AGCGCAGCTG CCTGAGCAGC 
CTGCAGTTTG TGTCGCCTCT CTACTTTGTG AGCTTCGTAC TCACAGCGCA GTTTGTGCTC 
ATCAACGTGG TGGTGGCCGT GCTGATGAAA CATCTGGATG ACAGCAACAA GGAGGCCCAG 
GAGGATGCAG AGATGGATGC TGAGATCGAG CTGGAGATGG CCCATGGCCT AGGCCCCGGG 
CCCGGCCCCT GTCCCTGTCC CTGTCCCTGC CCCTGCCCCT GTCCCTGTCC TGGCCCGAGG 
ATGCCCACCA GTTCACCGGG GGCTCCAGGC CGAGGATCAG GAGGGGCAGG CGTTGGAGGC 
GACACTGAGA GCCACCTGTG CCGGCACTGC TATTCTCCAG CCCAGGACTC CTTGGAGGGG 
GAGCTGACCA TCATTGACAA CCTGTCTGGC TCCATCTTCC ACCACTACTC CTCACCTGCC 
GGCTGTGACA AGTGTCACCA TGACAAGCAA GAGGGACCTG TTGCTCAGCG CTCCAGGCCC 
AGGCTGCCCC ATCCCAGCCC CAGGGTGACC CGAGGCCACC ACCTCCCCCC GCAGGTGCAG 
CTGGCTGAGA CAGAGGCCTT CTCCCTGAAC TCAGACAGGT CCTCGTCCGT CCTGCTGGGG 
GATGACCTGA GTCTTGAGGA CCCCACGGCC TGCCCGCAGG GCCCCAAGGA GAGCAAGGGT 
GAGCTAGAGC CTCCGGAGCC CATGCAGGCT GGAGACCTGG ATGAATGCTT CTTCCCCTTT 
GCCGGTGAGC CAGTGTCTGC AGGCCCAGAG AGCCTGCTGT GCGAGATGGG GGCCATTCCA 
TTCAACCCCG TCCAGTCCTG GCTCAAACAT GAGAGCAACC AAGCACCCCC AAGCCCTTTC 
TCCCCGGATG GTTCCAGCCC TCTGCTGCAG ATGCCTGCTG AGTTCTTCCA CCCTGCTGTG 
TCTGCCAGCC AGAAGGGGCA GGAACCGGGC ATGAGTTCAG GGACCCTGCC CAAGATTGCA 
CTTCAGGGGT CCTGGGCATC CCTGAGGTCA CCGAGTGTCA ACTGCACCCT CTTGCGCCAG 
GCTACTGTGA GTGACACGTC TTTGGATGCC AGCCCTAGCA GCTCAGCGGG CAGCCTGCAG 
ACCACACTGG AGGACAGTCT GACCCTGAGT GACAGTCCTC GGCGTGCCCT GGGACCACCA 
GTCCAGGTGC CAGGGCCACG GGCTAGCCTG TCACCTGCCA CCCGGCGCCG CCTCAGCCTG 
CGGGGGCGTG GCCTGTTTAG TCTGCGTGGG CTGCGGGCCC ATCAGCGTAG CCACAGCAGC 
GGTGGCTCCA CCAGCCCTGG CTGCACTTAC CACGACTCCA TGGACCCCTC TGATGAGGAG 
GGCCGCGGGG GCGCAGGTGG TGGGGGCGCG GGCAGTGAGC ACTCCGAGAC CCTCAGCAGC 
CTGTCACTCA CATCTCTCTT CTGCCTACCA CCCACGCTGC CACCACCCGG TCTCACCCCA 
GCCAGGAAGT TCAGCAGTAC CAGCAGCCTG GCTGCTGGAC CTGGCCGTCC TGGCGCCACT 
GTCTCGGTCC GCGGCCTGGC CAGGAGTCCC TCATGGGCTG CAGACCGCAG CAAGGACCCA 
CCAGGCCAGG CACAGCTGGC CTCCGGCTTC GGCTCCTCAG CACCCGAGCC ACAGCCACCT 
CCCGGGGAGT CAACGGATGC GGCGAGCAAA AGGAAGAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Growth differentiation factor-15 promotes glutamate release in medial prefrontal cortex of mice through upregulation of T-type calcium channels.
Scientific reports628653(2016 Jun)
Liu DD,Lu JM,Zhao QR,Hu C,Mei YA

Contribution of postsynaptic T-type calcium channels to parallel fibre-Purkinje cell synaptic responses.
The Journal of physiology594(4)915-36(2016 Feb)
Ly R,Bouvier G,Szapiro G,Prosser HM,Randall AD,Kano M,Sakimura K,Isope P,Barbour B,Feltz A

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Cacna1i cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

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