Nek4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nek4
Entrez Gene ID	23955
Full Name	NIMA (never in mitosis gene a)-related expressed kinase 4
Synonyms	Stk2
General protein information	Preferred Names NIMA (never in mitosis gene a)-related expressed kinase 4 Names serine/threonine-protein kinase Nek4 serine/threonine-protein kinase 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 14 Map Location: 14\|14 B

mRNA and Protein(s)

mRNA	Protein	Name
NM_011849.3	NP_035979.1	serine/threonine-protein kinase Nek4 isoform 1
NM_001308328.1	NP_001295257.1	serine/threonine-protein kinase Nek4 isoform 2
XM_011245068.2	XP_011243370.1	serine/threonine-protein kinase Nek4 isoform X1
XM_011245069.2	XP_011243371.1	serine/threonine-protein kinase Nek4 isoform X2
XM_011245068.3	XP_011243370.1	serine/threonine-protein kinase Nek4 isoform X1
XM_011245069.3	XP_011243371.1	serine/threonine-protein kinase Nek4 isoform X2

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	NEK4	XP_414252.2
Danio rerio (zebrafish)	nek4	NP_957306.1
Xenopus tropicalis (tropical clawed frog)	nek4	NP_001072768.1
Homo sapiens (human)	NEK4	NP_003148.2
Pan troglodytes (chimpanzee)	NEK4	XP_516519.4
Canis lupus familiaris (dog)	NEK4	XP_005632492.1
Bos taurus (cattle)	NEK4	NP_001095414.1
Mus musculus (house mouse)	Nek4	NP_035979.1
Rattus norvegicus (Norway rat)	Nek4	NP_001013152.2
	NEK4	XP_002802834.1

Gene Ontology
Bibliography

Related articles in PubMed

Nek4 status differentially alters sensitivity to distinct microtubule poisons.
Doles J, Hemann MT
Cancer research70(3)1033-41(2010 Feb)

Construction of a multi-functional cDNA library specific for mouse pancreatic islets and its application to microarray.
Nishimura M, Yokoi N, Miki T, Horikawa Y, Yoshioka H, Takeda J, Ohara O, Seino S
DNA research : an international journal for rapid publication of reports on genes and genomes11(5)315-23(2004 Oct)

Exploration of the cell-cycle genes found within the RIKEN FANTOM2 data set.
Forrest AR, Taylor D, Grimmond S, ,
Genome research13(6B)1366-75(2003 Jun)

Activity and substrate specificity of the murine STK2 Serine/Threonine kinase that is structurally related to the mitotic regulator protein NIMA of Aspergillus nidulans.
Hayashi K, Igarashi H, Ogawa M, Sakaguchi N
Biochemical and biophysical research communications264(2)449-56(1999 Oct)

NIMA-related kinases: isolation and characterization of murine nek3 and nek4 cDNAs, and chromosomal localization of nek1, nek2 and nek3.
Chen A, Yanai A, Arama E, Kilfin G, Motro B
Gene234(1)127-37(1999 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that after Taxol treatment, Nek4 promoted microtubule outgrowth, whereas Nek4 deficiency impaired G(2)-M arrest and decreased formation of mitotic-like asters.
Title: Nek4 status differentially alters sensitivity to distinct microtubule poisons.

The following Nek4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nek4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu69146	NM_001308328.1 Latest version!	Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 4 (Nek4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu08980	NM_011849.3 Latest version!	Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 4 (Nek4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
OMu69144	XM_011245068.3 Latest version!	Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 4 (Nek4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu69145	XM_011245069.3 Latest version!	Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 4 (Nek4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OMu69144	XM_011245068.2	Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 4 (Nek4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu69145	XM_011245069.2	Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 4 (Nek4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nek4 ( XM_011245069.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011243371.1 Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 4 (Nek4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu69145
Clone ID Related Accession (Same CDS sequence)	XM_011245069.2 , XM_011245069.3
Accession Version	XM_011245069.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2133bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase Nek4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011245069.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTTCT GCGAAGGAGG TGATCTGTAC CGGAAGCTCA AGGAGCAGAA AGGGCAGCTT 
CTGCCTGAGA GTCAGGTGGT GGAGTGGTTT GTCCAGATCG CCATGGCCTT GCAGTATTTA 
CATGAGAAAC ACATCCTTCA TCGAGATCTG AAAACTCAGA ATGTCTTCTT AACAAGAACA 
AACATCATCA AAGTGGGGGA CCTAGGAATT GCCCGCGTCT TAGAGAATCA CGGTGACATG 
GCGAGCACCC TCATTGGCAC ACCCTATTAC ATGAGCCCTG AGCTGTTCTC CAACAAACCC 
TACAACTATA AGTCGGATGT TTGGGCTTTG GGATGCTGTG TATATGAAAT GGCCACCCTG 
AAGCACGCTT TTAATGCAAA AGACATGAAT TCTTTAGTTT ATCGGATTAT TGAAGGAAAG 
TTGCCGCCAA TGCCAAAAGT TTACAGCACA GAGCTGGCTG AGCTGATAAG AACAATGCTG 
AGCAGAAGGC CTGAGGAGAG GCCCTCTGTG CGGAGCATCC TGAGGCAGCC CTACATAAAA 
CACCACATCT CCTTGTTCTT GGAGGCCACC AAGGCAAAAA CTTCCAAAAA TAATGTTAAA 
AACTGTGACT CCAGAGCCAA GCCTGTTGCT GCTGTAGTTT CCAGAAAGGA AGAATCAAAT 
ACTGACGTGA TACACTACCA GCCACGCTCT TCTGAGGGCT CTGCTTTGCA TGTCATGGGT 
GAAGATAAAT GTTTGTCCCA AGAGAAACCA GTGGACATTG GTCCCTTGAG ATCACCGGCT 
AGTCTTGAAG GCCACACTGG CAAACAAGAC ATGAACAATA CTGGGGAGTC ATGTGCCACA 
ATCAGTAGGA TAAATATCGA TATCTTACCT GCAGAAAGGA GGGACTCAGC GAACGCTGGT 
GTAGTTCAGG AGAGCCAACC ACAACACGTG GATGCCGCTG ATGAAGTAGA CAGTCAGTGC 
AGTATTTCTC AAGAGAAGGA GAGGCTACAG GGCAATACCA AGTCCAGTGA TCAGCCTGGA 
AATCTCCTTC CCAGACGGTC CTCTGATGGT GGTGATGGGG AAGGGAGTGA ACTGGTGAAG 
CCATTGTATC CCTCAAATAA AGACCAAAAG CCAGACCAGG ATCAAGTTAC TGGTATTATA 
GAAAATCAGG ACAGCATCCA CCCAAGATCC CAGCCACACA GCTCTATGTC TGAACCTTCT 
TTGTCCCGGC AGCGAAGGCA GAAAAAAAGG GAACAGACTG CCCATAGTCG TACAAAGAGC 
CAGTTCCAAG AATTACCCCC TCGGCTTTTG CCTTCTTATC CTGGTATTGG GAAAGTGGAT 
ATCATAGCAA CACAGCAAAA TGATGGAAAC CAAGGTGGAC CTGTGGCTGG GTGTGTAAAC 
AGTTCAAGGA CCAGCTCAAC AGCATCAGCA AAGGATCGAC CATTATCAGC CAGAGAGAGG 
AGACGGCTAA AGCAGTCACA GGAGGAGATG CTGCCCTCAG GCCCTGCAGT TCAGAGAACT 
CCAAGTGCAG TTGAGCCACT GAAACCACAG GAAGAAGACC AACCCATCCC TGCCCAACGA 
TTCTCTTCTG ACTGCAGCAT CACTCAGATG AATCACACAC TCCCGAGGGA AAAGGAGAAG 
AGGCTGATGC ATGGCCTGTC TGAGGATGAG TTAAGCTCTT CTACAAGCTC AACTGATAAG 
TCAGATGGGG ATTCCAGGGA GGGGAAGAGT CATACAAATG AAATGAAAGA CTTGGTACAG 
CTGATGACTC AGACTCTGAG GCTGGAAGCT AAGGAGAGCT GTGAAGATCT CCAGGTCCTA 
AACCCAGGGT CAGAATTCAG ACTTCATCGA AAGTATCGAG ACACGCTGGT GCTTCATGGC 
AAGGTTGCAG AAGAGGTGGA ACCCCACTGT ACGGAGCTGC CTACGGGTAT CATACCAGGT 
TCTGAAAAGA TCAGGAGAAT CGTTGAAGTC TTAAGAGCTG ATGTAATCCA GGGCCTGGGG 
ATTCAACTCT TAGAGCAGGT GTTTGATCTT TTGGGAGAGG AAGATGAGTT GGAGAGAGAG 
GCACGATTAC AGGAGCACAT GGGAGACAAG TATACAACTT ACTGTGTGAA AGCTCGCCAA 
TTGAAATTTT TTGAAGAAAA TGTGAGTTTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011243371.1
CDS	597..2729
Translation	MGFCEGGDLYRKLKEQKGQLLPESQVVEWFVQIAMALQYLHEKHILHRDLKTQNVFLTRTNIIKVGDLGIARVLENHGDMASTLIGTPYYMSPELFSNKPYNYKSDVWALGCCVYEMATLKHAFNAKDMNSLVYRIIEGKLPPMPKVYSTELAELIRTMLSRRPEERPSVRSILRQPYIKHHISLFLEATKAKTSKNNVKNCDSRAKPVAAVVSRKEESNTDVIHYQPRSSEGSALHVMGEDKCLSQEKPVDIGPLRSPASLEGHTGKQDMNNTGESCATISRINIDILPAERRDSANAGVVQESQPQHVDAADEVDSQCSISQEKERLQGNTKSSDQPGNLLPRRSSDGGDGEGSELVKPLYPSNKDQKPDQDQVTGIIENQDSIHPRSQPHSSMSEPSLSRQRRQKKREQTAHSRTKSQFQELPPRLLPSYPGIGKVDIIATQQNDGNQGGPVAGCVNSSRTSSTASAKDRPLSARERRRLKQSQEEMLPSGPAVQRTPSAVEPLKPQEEDQPIPAQRFSSDCSITQMNHTLPREKEKRLMHGLSEDELSSSTSSTDKSDGDSREGKSHTNEMKDLVQLMTQTLRLEAKESCEDLQVLNPGSEFRLHRKYRDTLVLHGKVAEEVEPHCTELPTGIIPGSEKIRRIVEVLRADVIQGLGIQLLEQVFDLLGEEDELEREARLQEHMGDKYTTYCVKARQLKFFEENVSF

Target ORF information:

RefSeq Version	XM_011245069.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 4 (Nek4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011245069.2

ORF Insert Sequence:

ATGGGCTTCT GCGAAGGAGG TGATCTGTAC CGGAAGCTCA AGGAGCAGAA AGGGCAGCTT 
CTGCCTGAGA GTCAGGTGGT GGAGTGGTTT GTCCAGATCG CCATGGCCTT GCAGTATTTA 
CATGAGAAAC ACATCCTTCA TCGAGATCTG AAAACTCAGA ATGTCTTCTT AACAAGAACA 
AACATCATCA AAGTGGGGGA CCTAGGAATT GCCCGCGTCT TAGAGAATCA CGGTGACATG 
GCGAGCACCC TCATTGGCAC ACCCTATTAC ATGAGCCCTG AGCTGTTCTC CAACAAACCC 
TACAACTATA AGTCGGATGT TTGGGCTTTG GGATGCTGTG TATATGAAAT GGCCACCCTG 
AAGCACGCTT TTAATGCAAA AGACATGAAT TCTTTAGTTT ATCGGATTAT TGAAGGAAAG 
TTGCCGCCAA TGCCAAAAGT TTACAGCACA GAGCTGGCTG AGCTGATAAG AACAATGCTG 
AGCAGAAGGC CTGAGGAGAG GCCCTCTGTG CGGAGCATCC TGAGGCAGCC CTACATAAAA 
CACCACATCT CCTTGTTCTT GGAGGCCACC AAGGCAAAAA CTTCCAAAAA TAATGTTAAA 
AACTGTGACT CCAGAGCCAA GCCTGTTGCT GCTGTAGTTT CCAGAAAGGA AGAATCAAAT 
ACTGACGTGA TACACTACCA GCCACGCTCT TCTGAGGGCT CTGCTTTGCA TGTCATGGGT 
GAAGATAAAT GTTTGTCCCA AGAGAAACCA GTGGACATTG GTCCCTTGAG ATCACCGGCT 
AGTCTTGAAG GCCACACTGG CAAACAAGAC ATGAACAATA CTGGGGAGTC ATGTGCCACA 
ATCAGTAGGA TAAATATCGA TATCTTACCT GCAGAAAGGA GGGACTCAGC GAACGCTGGT 
GTAGTTCAGG AGAGCCAACC ACAACACGTG GATGCCGCTG ATGAAGTAGA CAGTCAGTGC 
AGTATTTCTC AAGAGAAGGA GAGGCTACAG GGCAATACCA AGTCCAGTGA TCAGCCTGGA 
AATCTCCTTC CCAGACGGTC CTCTGATGGT GGTGATGGGG AAGGGAGTGA ACTGGTGAAG 
CCATTGTATC CCTCAAATAA AGACCAAAAG CCAGACCAGG ATCAAGTTAC TGGTATTATA 
GAAAATCAGG ACAGCATCCA CCCAAGATCC CAGCCACACA GCTCTATGTC TGAACCTTCT 
TTGTCCCGGC AGCGAAGGCA GAAAAAAAGG GAACAGACTG CCCATAGTCG TACAAAGAGC 
CAGTTCCAAG AATTACCCCC TCGGCTTTTG CCTTCTTATC CTGGTATTGG GAAAGTGGAT 
ATCATAGCAA CACAGCAAAA TGATGGAAAC CAAGGTGGAC CTGTGGCTGG GTGTGTAAAC 
AGTTCAAGGA CCAGCTCAAC AGCATCAGCA AAGGATCGAC CATTATCAGC CAGAGAGAGG 
AGACGGCTAA AGCAGTCACA GGAGGAGATG CTGCCCTCAG GCCCTGCAGT TCAGAGAACT 
CCAAGTGCAG TTGAGCCACT GAAACCACAG GAAGAAGACC AACCCATCCC TGCCCAACGA 
TTCTCTTCTG ACTGCAGCAT CACTCAGATG AATCACACAC TCCCGAGGGA AAAGGAGAAG 
AGGCTGATGC ATGGCCTGTC TGAGGATGAG TTAAGCTCTT CTACAAGCTC AACTGATAAG 
TCAGATGGGG ATTCCAGGGA GGGGAAGAGT CATACAAATG AAATGAAAGA CTTGGTACAG 
CTGATGACTC AGACTCTGAG GCTGGAAGCT AAGGAGAGCT GTGAAGATCT CCAGGTCCTA 
AACCCAGGGT CAGAATTCAG ACTTCATCGA AAGTATCGAG ACACGCTGGT GCTTCATGGC 
AAGGTTGCAG AAGAGGTGGA ACCCCACTGT ACGGAGCTGC CTACGGGTAT CATACCAGGT 
TCTGAAAAGA TCAGGAGAAT CGTTGAAGTC TTAAGAGCTG ATGTAATCCA GGGCCTGGGG 
ATTCAACTCT TAGAGCAGGT GTTTGATCTT TTGGGAGAGG AAGATGAGTT GGAGAGAGAG 
GCACGATTAC AGGAGCACAT GGGAGACAAG TATACAACTT ACTGTGTGAA AGCTCGCCAA 
TTGAAATTTT TTGAAGAAAA TGTGAGTTTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nek4 ( NM_001308328.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001295257.1 Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 4 (Nek4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu69146
Clone ID Related Accession (Same CDS sequence)	NM_001308328.1
Accession Version	NM_001308328.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase Nek4 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK078809.1, AK149584.1 and BP762751.1. On Apr 29, 2015 this sequence version replaced XM_006519011.2. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK149584.1, AK078809.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCCAGG CCGCATACTG CTACATGCGG GTCGTGGGCA GGGGTAGTTA CGGGGAGGTG 
ACGCTCGTGA AGCATCGGCG AGACGGCAAG CAGTATGTCA TCAAAAAGCT GAACCTCCGA 
AATGCCTCCA GCCGAGAGCG TCGAGCTGCT GAGCAGGAAG CTCAGCTCCT ATCTCAGTTG 
AAGCACCCCA ACATTGTCAC CTACAAAGAA TCATGGGAGG GAGGAGACGG TCTGCTCTAC 
ATTGTCATGG GCTTCTGCGA AGGAGGTGAT CTGTACCGGA AGCTCAAGGA GCAGAAAGGG 
CAGCTTCTGC CTGAGAGTCA GGTGGTGGAG TGGTTTGTCC AGATCGCCAT GGCCTTGCAG 
TATTTACATG AGAAACACAT CCTTCATCGA GATCTGAAAA CTCAGAATGT CTTCTTAACA 
AGAACAAACA TCATCAAAGT GGGGGACCTA GGAATTGCCC GCGTCTTAGA GAATCACGGT 
GACATGGCGA GCACCCTCAT TGGCACACCC TATTACATGA GCCCTGAGCT GTTCTCCAAC 
AAACCCTACA ACTATAAGTC GGATGTTTGG GCTTTGGGAT GCTGTGTATA TGAAATGGCC 
ACCCTGAAGC ACGCTTTTAA TGCAAAAGAC ATGAATTCTT TAGTTTATCG GATTATTGAA 
GGAAAGTTGC CGCCAATGCC AAAAGTTTAC AGCACAGAGC TGGCTGAGCT GATAAGAACA 
ATGCTGAGCA GAAGGCCTGA GGAGAGGCCC TCTGTGCGGA GCATCCTGAG GCAGCCCTAC 
ATAAAACACC ACATCTCCTT GTTCTTGGAG GCCACCAAGG CAAAAACTTC CAAAAATAAT 
GTTAAAAACT GTGACTCCAG AGCCAAGCCT GTTGCTGCTG TAGTTTCCAG AAAGGAAGAA 
TCAAATACTG ACGTGATACA CTACCAGCCA CGCTCTTCTG AGGGCTCTGC TTTGCATGTC 
ATGGGTGAAG ATAAATGTTT GTCCCAAGAG AAACCAGTGG ACATTGGTCC CTTGAGATCA 
CCGGCTAGTC TTGAAGGCCA CACTGGCAAA CAAGACATGA ACAATACTGG GGAGTCATGT 
GCCACAATCA GTAGGATAAA TATCGATATC TTACCTGCAG AAAGGAGGGA CTCAGCGAAC 
GCTGGTGTAG TTCAGGAGAG CCAACCACAA CACGTGGATG CCGCTGATGA AGTAGACAGT 
CAGTGCAGTA TTTCTCAAGA GAAGGAGAGG CTACAGGGCA ATACCAAGTC CAGTGATCAG 
CCTGGAAATC TCCTTCCCAG ACGGTCCTCT GATGGTGGTG ATGGGGAAGG GAGTGAACTG 
GTGAAGCCAT TGTATCCCTC AAATAAAGAC CAAAAGCCAG ACCAGTTCCA AGAATTACCC 
CCTCGGCTTT TGCCTTCTTA TCCTGGTATT GGGAAAGTGG ATATCATAGC AACACAGCAA 
AATGATGGAA ACCAAGGTGG ACCTGTGGCT GGGTGTGTAA ACAGTTCAAG GACCAGCTCA 
ACAGCATCAG CAAAGGATCG ACCATTATCA GCCAGAGAGA GGAGACGGCT AAAGCAGTCA 
CAGGAGGAGA TGCTGCCCTC AGGCCCTGCA GTTCAGAGAA CTCCAAGTGC AGTTGAGCCA 
CTGAAACCAC AGGAAGAAGA CCAACCCATC CCTGCCCAAC GATTCTCTTC TGACTGCAGC 
ATCACTCAGA TGAATCACAC ACTCCCGAGG GAAAAGGAGA AGAGGCTGAT GCATGGCCTG 
TCTGAGGATG AGTTAAGCTC TTCTACAAGC TCAACTGATA AGTCAGATGG GGATTCCAGG 
GAGGGGAAGA GTCATACAAA TGAAATGAAA GACTTGGTAC AGCTGATGAC TCAGACTCTG 
AGGCTGGAAG CTAAGGAGAG CTGTGAAGAT CTCCAGGTCC TAAACCCAGG GTCAGAATTC 
AGACTTCATC GAAAGTATCG AGACACGCTG GTGCTTCATG GCAAGGTTGC AGAAGAGGTG 
GAACCCCACT GTACGGAGCT GCCTACGGGT ATCATACCAG GTTCTGAAAA GATCAGGAGA 
ATCGTTGAAG TCTTAAGAGC TGATGTAATC CAGGGCCTGG GGATTCAACT CTTAGAGCAG 
GTGTTTGATC TTTTGGGAGA GGAAGATGAG TTGGAGAGAG AGGCACGATT ACAGGAGCAC 
ATGGGAGACA AGTATACAAC TTACTGTGTG AAAGCTCGCC AATTGAAATT TTTTGAAGAA 
AATGTGAGTT TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001295257.1
CDS	250..2484
Translation	MPQAAYCYMRVVGRGSYGEVTLVKHRRDGKQYVIKKLNLRNASSRERRAAEQEAQLLSQLKHPNIVTYKESWEGGDGLLYIVMGFCEGGDLYRKLKEQKGQLLPESQVVEWFVQIAMALQYLHEKHILHRDLKTQNVFLTRTNIIKVGDLGIARVLENHGDMASTLIGTPYYMSPELFSNKPYNYKSDVWALGCCVYEMATLKHAFNAKDMNSLVYRIIEGKLPPMPKVYSTELAELIRTMLSRRPEERPSVRSILRQPYIKHHISLFLEATKAKTSKNNVKNCDSRAKPVAAVVSRKEESNTDVIHYQPRSSEGSALHVMGEDKCLSQEKPVDIGPLRSPASLEGHTGKQDMNNTGESCATISRINIDILPAERRDSANAGVVQESQPQHVDAADEVDSQCSISQEKERLQGNTKSSDQPGNLLPRRSSDGGDGEGSELVKPLYPSNKDQKPDQFQELPPRLLPSYPGIGKVDIIATQQNDGNQGGPVAGCVNSSRTSSTASAKDRPLSARERRRLKQSQEEMLPSGPAVQRTPSAVEPLKPQEEDQPIPAQRFSSDCSITQMNHTLPREKEKRLMHGLSEDELSSSTSSTDKSDGDSREGKSHTNEMKDLVQLMTQTLRLEAKESCEDLQVLNPGSEFRLHRKYRDTLVLHGKVAEEVEPHCTELPTGIIPGSEKIRRIVEVLRADVIQGLGIQLLEQVFDLLGEEDELEREARLQEHMGDKYTTYCVKARQLKFFEENVSF

Target ORF information:

RefSeq Version	NM_001308328.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 4 (Nek4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001308328.1

ORF Insert Sequence:

ATGCCCCAGG CCGCATACTG CTACATGCGG GTCGTGGGCA GGGGTAGTTA CGGGGAGGTG 
ACGCTCGTGA AGCATCGGCG AGACGGCAAG CAGTATGTCA TCAAAAAGCT GAACCTCCGA 
AATGCCTCCA GCCGAGAGCG TCGAGCTGCT GAGCAGGAAG CTCAGCTCCT ATCTCAGTTG 
AAGCACCCCA ACATTGTCAC CTACAAAGAA TCATGGGAGG GAGGAGACGG TCTGCTCTAC 
ATTGTCATGG GCTTCTGCGA AGGAGGTGAT CTGTACCGGA AGCTCAAGGA GCAGAAAGGG 
CAGCTTCTGC CTGAGAGTCA GGTGGTGGAG TGGTTTGTCC AGATCGCCAT GGCCTTGCAG 
TATTTACATG AGAAACACAT CCTTCATCGA GATCTGAAAA CTCAGAATGT CTTCTTAACA 
AGAACAAACA TCATCAAAGT GGGGGACCTA GGAATTGCCC GCGTCTTAGA GAATCACGGT 
GACATGGCGA GCACCCTCAT TGGCACACCC TATTACATGA GCCCTGAGCT GTTCTCCAAC 
AAACCCTACA ACTATAAGTC GGATGTTTGG GCTTTGGGAT GCTGTGTATA TGAAATGGCC 
ACCCTGAAGC ACGCTTTTAA TGCAAAAGAC ATGAATTCTT TAGTTTATCG GATTATTGAA 
GGAAAGTTGC CGCCAATGCC AAAAGTTTAC AGCACAGAGC TGGCTGAGCT GATAAGAACA 
ATGCTGAGCA GAAGGCCTGA GGAGAGGCCC TCTGTGCGGA GCATCCTGAG GCAGCCCTAC 
ATAAAACACC ACATCTCCTT GTTCTTGGAG GCCACCAAGG CAAAAACTTC CAAAAATAAT 
GTTAAAAACT GTGACTCCAG AGCCAAGCCT GTTGCTGCTG TAGTTTCCAG AAAGGAAGAA 
TCAAATACTG ACGTGATACA CTACCAGCCA CGCTCTTCTG AGGGCTCTGC TTTGCATGTC 
ATGGGTGAAG ATAAATGTTT GTCCCAAGAG AAACCAGTGG ACATTGGTCC CTTGAGATCA 
CCGGCTAGTC TTGAAGGCCA CACTGGCAAA CAAGACATGA ACAATACTGG GGAGTCATGT 
GCCACAATCA GTAGGATAAA TATCGATATC TTACCTGCAG AAAGGAGGGA CTCAGCGAAC 
GCTGGTGTAG TTCAGGAGAG CCAACCACAA CACGTGGATG CCGCTGATGA AGTAGACAGT 
CAGTGCAGTA TTTCTCAAGA GAAGGAGAGG CTACAGGGCA ATACCAAGTC CAGTGATCAG 
CCTGGAAATC TCCTTCCCAG ACGGTCCTCT GATGGTGGTG ATGGGGAAGG GAGTGAACTG 
GTGAAGCCAT TGTATCCCTC AAATAAAGAC CAAAAGCCAG ACCAGTTCCA AGAATTACCC 
CCTCGGCTTT TGCCTTCTTA TCCTGGTATT GGGAAAGTGG ATATCATAGC AACACAGCAA 
AATGATGGAA ACCAAGGTGG ACCTGTGGCT GGGTGTGTAA ACAGTTCAAG GACCAGCTCA 
ACAGCATCAG CAAAGGATCG ACCATTATCA GCCAGAGAGA GGAGACGGCT AAAGCAGTCA 
CAGGAGGAGA TGCTGCCCTC AGGCCCTGCA GTTCAGAGAA CTCCAAGTGC AGTTGAGCCA 
CTGAAACCAC AGGAAGAAGA CCAACCCATC CCTGCCCAAC GATTCTCTTC TGACTGCAGC 
ATCACTCAGA TGAATCACAC ACTCCCGAGG GAAAAGGAGA AGAGGCTGAT GCATGGCCTG 
TCTGAGGATG AGTTAAGCTC TTCTACAAGC TCAACTGATA AGTCAGATGG GGATTCCAGG 
GAGGGGAAGA GTCATACAAA TGAAATGAAA GACTTGGTAC AGCTGATGAC TCAGACTCTG 
AGGCTGGAAG CTAAGGAGAG CTGTGAAGAT CTCCAGGTCC TAAACCCAGG GTCAGAATTC 
AGACTTCATC GAAAGTATCG AGACACGCTG GTGCTTCATG GCAAGGTTGC AGAAGAGGTG 
GAACCCCACT GTACGGAGCT GCCTACGGGT ATCATACCAG GTTCTGAAAA GATCAGGAGA 
ATCGTTGAAG TCTTAAGAGC TGATGTAATC CAGGGCCTGG GGATTCAACT CTTAGAGCAG 
GTGTTTGATC TTTTGGGAGA GGAAGATGAG TTGGAGAGAG AGGCACGATT ACAGGAGCAC 
ATGGGAGACA AGTATACAAC TTACTGTGTG AAAGCTCGCC AATTGAAATT TTTTGAAGAA 
AATGTGAGTT TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nek4 ( XM_011245068.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011243370.1 Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 4 (Nek4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu69144
Clone ID Related Accession (Same CDS sequence)	XM_011245068.2 , XM_011245068.3
Accession Version	XM_011245068.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2358bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase Nek4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011245068.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCACC CCACCCAGCC AGTCTCATGC TTTTTGATCA TCCTCCTGCC TCGTCTTCGA 
TGTATTATAC TGTATGTCAT CAAAAAGCTG AACCTCCGAA ATGCCTCCAG CCGAGAGCGT 
CGAGCTGCTG AGCAGGAAGC TCAGCTCCTA TCTCAGTTGA AGCACCCCAA CATTGTCACC 
TACAAAGAAT CATGGGAGGG AGGAGACGGT CTGCTCTACA TTGTCATGGG CTTCTGCGAA 
GGAGGTGATC TGTACCGGAA GCTCAAGGAG CAGAAAGGGC AGCTTCTGCC TGAGAGTCAG 
GTGGTGGAGT GGTTTGTCCA GATCGCCATG GCCTTGCAGT ATTTACATGA GAAACACATC 
CTTCATCGAG ATCTGAAAAC TCAGAATGTC TTCTTAACAA GAACAAACAT CATCAAAGTG 
GGGGACCTAG GAATTGCCCG CGTCTTAGAG AATCACGGTG ACATGGCGAG CACCCTCATT 
GGCACACCCT ATTACATGAG CCCTGAGCTG TTCTCCAACA AACCCTACAA CTATAAGTCG 
GATGTTTGGG CTTTGGGATG CTGTGTATAT GAAATGGCCA CCCTGAAGCA CGCTTTTAAT 
GCAAAAGACA TGAATTCTTT AGTTTATCGG ATTATTGAAG GAAAGTTGCC GCCAATGCCA 
AAAGTTTACA GCACAGAGCT GGCTGAGCTG ATAAGAACAA TGCTGAGCAG AAGGCCTGAG 
GAGAGGCCCT CTGTGCGGAG CATCCTGAGG CAGCCCTACA TAAAACACCA CATCTCCTTG 
TTCTTGGAGG CCACCAAGGC AAAAACTTCC AAAAATAATG TTAAAAACTG TGACTCCAGA 
GCCAAGCCTG TTGCTGCTGT AGTTTCCAGA AAGGAAGAAT CAAATACTGA CGTGATACAC 
TACCAGCCAC GCTCTTCTGA GGGCTCTGCT TTGCATGTCA TGGGTGAAGA TAAATGTTTG 
TCCCAAGAGA AACCAGTGGA CATTGGTCCC TTGAGATCAC CGGCTAGTCT TGAAGGCCAC 
ACTGGCAAAC AAGACATGAA CAATACTGGG GAGTCATGTG CCACAATCAG TAGGATAAAT 
ATCGATATCT TACCTGCAGA AAGGAGGGAC TCAGCGAACG CTGGTGTAGT TCAGGAGAGC 
CAACCACAAC ACGTGGATGC CGCTGATGAA GTAGACAGTC AGTGCAGTAT TTCTCAAGAG 
AAGGAGAGGC TACAGGGCAA TACCAAGTCC AGTGATCAGC CTGGAAATCT CCTTCCCAGA 
CGGTCCTCTG ATGGTGGTGA TGGGGAAGGG AGTGAACTGG TGAAGCCATT GTATCCCTCA 
AATAAAGACC AAAAGCCAGA CCAGGATCAA GTTACTGGTA TTATAGAAAA TCAGGACAGC 
ATCCACCCAA GATCCCAGCC ACACAGCTCT ATGTCTGAAC CTTCTTTGTC CCGGCAGCGA 
AGGCAGAAAA AAAGGGAACA GACTGCCCAT AGTCGTACAA AGAGCCAGTT CCAAGAATTA 
CCCCCTCGGC TTTTGCCTTC TTATCCTGGT ATTGGGAAAG TGGATATCAT AGCAACACAG 
CAAAATGATG GAAACCAAGG TGGACCTGTG GCTGGGTGTG TAAACAGTTC AAGGACCAGC 
TCAACAGCAT CAGCAAAGGA TCGACCATTA TCAGCCAGAG AGAGGAGACG GCTAAAGCAG 
TCACAGGAGG AGATGCTGCC CTCAGGCCCT GCAGTTCAGA GAACTCCAAG TGCAGTTGAG 
CCACTGAAAC CACAGGAAGA AGACCAACCC ATCCCTGCCC AACGATTCTC TTCTGACTGC 
AGCATCACTC AGATGAATCA CACACTCCCG AGGGAAAAGG AGAAGAGGCT GATGCATGGC 
CTGTCTGAGG ATGAGTTAAG CTCTTCTACA AGCTCAACTG ATAAGTCAGA TGGGGATTCC 
AGGGAGGGGA AGAGTCATAC AAATGAAATG AAAGACTTGG TACAGCTGAT GACTCAGACT 
CTGAGGCTGG AAGCTAAGGA GAGCTGTGAA GATCTCCAGG TCCTAAACCC AGGGTCAGAA 
TTCAGACTTC ATCGAAAGTA TCGAGACACG CTGGTGCTTC ATGGCAAGGT TGCAGAAGAG 
GTGGAACCCC ACTGTACGGA GCTGCCTACG GGTATCATAC CAGGTTCTGA AAAGATCAGG 
AGAATCGTTG AAGTCTTAAG AGCTGATGTA ATCCAGGGCC TGGGGATTCA ACTCTTAGAG 
CAGGTGTTTG ATCTTTTGGG AGAGGAAGAT GAGTTGGAGA GAGAGGCACG ATTACAGGAG 
CACATGGGAG ACAAGTATAC AACTTACTGT GTGAAAGCTC GCCAATTGAA ATTTTTTGAA 
GAAAATGTGA GTTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011243370.1
CDS	1171..3528
Translation	MDHPTQPVSCFLIILLPRLRCIILYVIKKLNLRNASSRERRAAEQEAQLLSQLKHPNIVTYKESWEGGDGLLYIVMGFCEGGDLYRKLKEQKGQLLPESQVVEWFVQIAMALQYLHEKHILHRDLKTQNVFLTRTNIIKVGDLGIARVLENHGDMASTLIGTPYYMSPELFSNKPYNYKSDVWALGCCVYEMATLKHAFNAKDMNSLVYRIIEGKLPPMPKVYSTELAELIRTMLSRRPEERPSVRSILRQPYIKHHISLFLEATKAKTSKNNVKNCDSRAKPVAAVVSRKEESNTDVIHYQPRSSEGSALHVMGEDKCLSQEKPVDIGPLRSPASLEGHTGKQDMNNTGESCATISRINIDILPAERRDSANAGVVQESQPQHVDAADEVDSQCSISQEKERLQGNTKSSDQPGNLLPRRSSDGGDGEGSELVKPLYPSNKDQKPDQDQVTGIIENQDSIHPRSQPHSSMSEPSLSRQRRQKKREQTAHSRTKSQFQELPPRLLPSYPGIGKVDIIATQQNDGNQGGPVAGCVNSSRTSSTASAKDRPLSARERRRLKQSQEEMLPSGPAVQRTPSAVEPLKPQEEDQPIPAQRFSSDCSITQMNHTLPREKEKRLMHGLSEDELSSSTSSTDKSDGDSREGKSHTNEMKDLVQLMTQTLRLEAKESCEDLQVLNPGSEFRLHRKYRDTLVLHGKVAEEVEPHCTELPTGIIPGSEKIRRIVEVLRADVIQGLGIQLLEQVFDLLGEEDELEREARLQEHMGDKYTTYCVKARQLKFFEENVSF

Target ORF information:

RefSeq Version	XM_011245068.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 4 (Nek4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011245068.2

ORF Insert Sequence:

ATGGACCACC CCACCCAGCC AGTCTCATGC TTTTTGATCA TCCTCCTGCC TCGTCTTCGA 
TGTATTATAC TGTATGTCAT CAAAAAGCTG AACCTCCGAA ATGCCTCCAG CCGAGAGCGT 
CGAGCTGCTG AGCAGGAAGC TCAGCTCCTA TCTCAGTTGA AGCACCCCAA CATTGTCACC 
TACAAAGAAT CATGGGAGGG AGGAGACGGT CTGCTCTACA TTGTCATGGG CTTCTGCGAA 
GGAGGTGATC TGTACCGGAA GCTCAAGGAG CAGAAAGGGC AGCTTCTGCC TGAGAGTCAG 
GTGGTGGAGT GGTTTGTCCA GATCGCCATG GCCTTGCAGT ATTTACATGA GAAACACATC 
CTTCATCGAG ATCTGAAAAC TCAGAATGTC TTCTTAACAA GAACAAACAT CATCAAAGTG 
GGGGACCTAG GAATTGCCCG CGTCTTAGAG AATCACGGTG ACATGGCGAG CACCCTCATT 
GGCACACCCT ATTACATGAG CCCTGAGCTG TTCTCCAACA AACCCTACAA CTATAAGTCG 
GATGTTTGGG CTTTGGGATG CTGTGTATAT GAAATGGCCA CCCTGAAGCA CGCTTTTAAT 
GCAAAAGACA TGAATTCTTT AGTTTATCGG ATTATTGAAG GAAAGTTGCC GCCAATGCCA 
AAAGTTTACA GCACAGAGCT GGCTGAGCTG ATAAGAACAA TGCTGAGCAG AAGGCCTGAG 
GAGAGGCCCT CTGTGCGGAG CATCCTGAGG CAGCCCTACA TAAAACACCA CATCTCCTTG 
TTCTTGGAGG CCACCAAGGC AAAAACTTCC AAAAATAATG TTAAAAACTG TGACTCCAGA 
GCCAAGCCTG TTGCTGCTGT AGTTTCCAGA AAGGAAGAAT CAAATACTGA CGTGATACAC 
TACCAGCCAC GCTCTTCTGA GGGCTCTGCT TTGCATGTCA TGGGTGAAGA TAAATGTTTG 
TCCCAAGAGA AACCAGTGGA CATTGGTCCC TTGAGATCAC CGGCTAGTCT TGAAGGCCAC 
ACTGGCAAAC AAGACATGAA CAATACTGGG GAGTCATGTG CCACAATCAG TAGGATAAAT 
ATCGATATCT TACCTGCAGA AAGGAGGGAC TCAGCGAACG CTGGTGTAGT TCAGGAGAGC 
CAACCACAAC ACGTGGATGC CGCTGATGAA GTAGACAGTC AGTGCAGTAT TTCTCAAGAG 
AAGGAGAGGC TACAGGGCAA TACCAAGTCC AGTGATCAGC CTGGAAATCT CCTTCCCAGA 
CGGTCCTCTG ATGGTGGTGA TGGGGAAGGG AGTGAACTGG TGAAGCCATT GTATCCCTCA 
AATAAAGACC AAAAGCCAGA CCAGGATCAA GTTACTGGTA TTATAGAAAA TCAGGACAGC 
ATCCACCCAA GATCCCAGCC ACACAGCTCT ATGTCTGAAC CTTCTTTGTC CCGGCAGCGA 
AGGCAGAAAA AAAGGGAACA GACTGCCCAT AGTCGTACAA AGAGCCAGTT CCAAGAATTA 
CCCCCTCGGC TTTTGCCTTC TTATCCTGGT ATTGGGAAAG TGGATATCAT AGCAACACAG 
CAAAATGATG GAAACCAAGG TGGACCTGTG GCTGGGTGTG TAAACAGTTC AAGGACCAGC 
TCAACAGCAT CAGCAAAGGA TCGACCATTA TCAGCCAGAG AGAGGAGACG GCTAAAGCAG 
TCACAGGAGG AGATGCTGCC CTCAGGCCCT GCAGTTCAGA GAACTCCAAG TGCAGTTGAG 
CCACTGAAAC CACAGGAAGA AGACCAACCC ATCCCTGCCC AACGATTCTC TTCTGACTGC 
AGCATCACTC AGATGAATCA CACACTCCCG AGGGAAAAGG AGAAGAGGCT GATGCATGGC 
CTGTCTGAGG ATGAGTTAAG CTCTTCTACA AGCTCAACTG ATAAGTCAGA TGGGGATTCC 
AGGGAGGGGA AGAGTCATAC AAATGAAATG AAAGACTTGG TACAGCTGAT GACTCAGACT 
CTGAGGCTGG AAGCTAAGGA GAGCTGTGAA GATCTCCAGG TCCTAAACCC AGGGTCAGAA 
TTCAGACTTC ATCGAAAGTA TCGAGACACG CTGGTGCTTC ATGGCAAGGT TGCAGAAGAG 
GTGGAACCCC ACTGTACGGA GCTGCCTACG GGTATCATAC CAGGTTCTGA AAAGATCAGG 
AGAATCGTTG AAGTCTTAAG AGCTGATGTA ATCCAGGGCC TGGGGATTCA ACTCTTAGAG 
CAGGTGTTTG ATCTTTTGGG AGAGGAAGAT GAGTTGGAGA GAGAGGCACG ATTACAGGAG 
CACATGGGAG ACAAGTATAC AACTTACTGT GTGAAAGCTC GCCAATTGAA ATTTTTTGAA 
GAAAATGTGA GTTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nek4 ( NM_011849.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035979.1 Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 4 (Nek4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu08980
Clone ID Related Accession (Same CDS sequence)	NM_011849.3
Accession Version	NM_011849.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2379bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase Nek4 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK078809.1, CN526970.1, AK149584.1 and BP762751.1. On Apr 29, 2015 this sequence version replaced NM_011849.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: BC057939.1, AF099067.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCCAGG CCGCATACTG CTACATGCGG GTCGTGGGCA GGGGTAGTTA CGGGGAGGTG 
ACGCTCGTGA AGCATCGGCG AGACGGCAAG CAGTATGTCA TCAAAAAGCT GAACCTCCGA 
AATGCCTCCA GCCGAGAGCG TCGAGCTGCT GAGCAGGAAG CTCAGCTCCT ATCTCAGTTG 
AAGCACCCCA ACATTGTCAC CTACAAAGAA TCATGGGAGG GAGGAGACGG TCTGCTCTAC 
ATTGTCATGG GCTTCTGCGA AGGAGGTGAT CTGTACCGGA AGCTCAAGGA GCAGAAAGGG 
CAGCTTCTGC CTGAGAGTCA GGTGGTGGAG TGGTTTGTCC AGATCGCCAT GGCCTTGCAG 
TATTTACATG AGAAACACAT CCTTCATCGA GATCTGAAAA CTCAGAATGT CTTCTTAACA 
AGAACAAACA TCATCAAAGT GGGGGACCTA GGAATTGCCC GCGTCTTAGA GAATCACGGT 
GACATGGCGA GCACCCTCAT TGGCACACCC TATTACATGA GCCCTGAGCT GTTCTCCAAC 
AAACCCTACA ACTATAAGTC GGATGTTTGG GCTTTGGGAT GCTGTGTATA TGAAATGGCC 
ACCCTGAAGC ACGCTTTTAA TGCAAAAGAC ATGAATTCTT TAGTTTATCG GATTATTGAA 
GGAAAGTTGC CGCCAATGCC AAAAGTTTAC AGCACAGAGC TGGCTGAGCT GATAAGAACA 
ATGCTGAGCA GAAGGCCTGA GGAGAGGCCC TCTGTGCGGA GCATCCTGAG GCAGCCCTAC 
ATAAAACACC ACATCTCCTT GTTCTTGGAG GCCACCAAGG CAAAAACTTC CAAAAATAAT 
GTTAAAAACT GTGACTCCAG AGCCAAGCCT GTTGCTGCTG TAGTTTCCAG AAAGGAAGAA 
TCAAATACTG ACGTGATACA CTACCAGCCA CGCTCTTCTG AGGGCTCTGC TTTGCATGTC 
ATGGGTGAAG ATAAATGTTT GTCCCAAGAG AAACCAGTGG ACATTGGTCC CTTGAGATCA 
CCGGCTAGTC TTGAAGGCCA CACTGGCAAA CAAGACATGA ACAATACTGG GGAGTCATGT 
GCCACAATCA GTAGGATAAA TATCGATATC TTACCTGCAG AAAGGAGGGA CTCAGCGAAC 
GCTGGTGTAG TTCAGGAGAG CCAACCACAA CACGTGGATG CCGCTGATGA AGTAGACAGT 
CAGTGCAGTA TTTCTCAAGA GAAGGAGAGG CTACAGGGCA ATACCAAGTC CAGTGATCAG 
CCTGGAAATC TCCTTCCCAG ACGGTCCTCT GATGGTGGTG ATGGGGAAGG GAGTGAACTG 
GTGAAGCCAT TGTATCCCTC AAATAAAGAC CAAAAGCCAG ACCAGGATCA AGTTACTGGT 
ATTATAGAAA ATCAGGACAG CATCCACCCA AGATCCCAGC CACACAGCTC TATGTCTGAA 
CCTTCTTTGT CCCGGCAGCG AAGGCAGAAA AAAAGGGAAC AGACTGCCCA TAGTCGTACA 
AAGAGCCAGT TCCAAGAATT ACCCCCTCGG CTTTTGCCTT CTTATCCTGG TATTGGGAAA 
GTGGATATCA TAGCAACACA GCAAAATGAT GGAAACCAAG GTGGACCTGT GGCTGGGTGT 
GTAAACAGTT CAAGGACCAG CTCAACAGCA TCAGCAAAGG ATCGACCATT ATCAGCCAGA 
GAGAGGAGAC GGCTAAAGCA GTCACAGGAG GAGATGCTGC CCTCAGGCCC TGCAGTTCAG 
AGAACTCCAA GTGCAGTTGA GCCACTGAAA CCACAGGAAG AAGACCAACC CATCCCTGCC 
CAACGATTCT CTTCTGACTG CAGCATCACT CAGATGAATC ACACACTCCC GAGGGAAAAG 
GAGAAGAGGC TGATGCATGG CCTGTCTGAG GATGAGTTAA GCTCTTCTAC AAGCTCAACT 
GATAAGTCAG ATGGGGATTC CAGGGAGGGG AAGAGTCATA CAAATGAAAT GAAAGACTTG 
GTACAGCTGA TGACTCAGAC TCTGAGGCTG GAAGCTAAGG AGAGCTGTGA AGATCTCCAG 
GTCCTAAACC CAGGGTCAGA ATTCAGACTT CATCGAAAGT ATCGAGACAC GCTGGTGCTT 
CATGGCAAGG TTGCAGAAGA GGTGGAACCC CACTGTACGG AGCTGCCTAC GGGTATCATA 
CCAGGTTCTG AAAAGATCAG GAGAATCGTT GAAGTCTTAA GAGCTGATGT AATCCAGGGC 
CTGGGGATTC AACTCTTAGA GCAGGTGTTT GATCTTTTGG GAGAGGAAGA TGAGTTGGAG 
AGAGAGGCAC GATTACAGGA GCACATGGGA GACAAGTATA CAACTTACTG TGTGAAAGCT 
CGCCAATTGA AATTTTTTGA AGAAAATGTG AGTTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035979.1
CDS	250..2628
Translation	MPQAAYCYMRVVGRGSYGEVTLVKHRRDGKQYVIKKLNLRNASSRERRAAEQEAQLLSQLKHPNIVTYKESWEGGDGLLYIVMGFCEGGDLYRKLKEQKGQLLPESQVVEWFVQIAMALQYLHEKHILHRDLKTQNVFLTRTNIIKVGDLGIARVLENHGDMASTLIGTPYYMSPELFSNKPYNYKSDVWALGCCVYEMATLKHAFNAKDMNSLVYRIIEGKLPPMPKVYSTELAELIRTMLSRRPEERPSVRSILRQPYIKHHISLFLEATKAKTSKNNVKNCDSRAKPVAAVVSRKEESNTDVIHYQPRSSEGSALHVMGEDKCLSQEKPVDIGPLRSPASLEGHTGKQDMNNTGESCATISRINIDILPAERRDSANAGVVQESQPQHVDAADEVDSQCSISQEKERLQGNTKSSDQPGNLLPRRSSDGGDGEGSELVKPLYPSNKDQKPDQDQVTGIIENQDSIHPRSQPHSSMSEPSLSRQRRQKKREQTAHSRTKSQFQELPPRLLPSYPGIGKVDIIATQQNDGNQGGPVAGCVNSSRTSSTASAKDRPLSARERRRLKQSQEEMLPSGPAVQRTPSAVEPLKPQEEDQPIPAQRFSSDCSITQMNHTLPREKEKRLMHGLSEDELSSSTSSTDKSDGDSREGKSHTNEMKDLVQLMTQTLRLEAKESCEDLQVLNPGSEFRLHRKYRDTLVLHGKVAEEVEPHCTELPTGIIPGSEKIRRIVEVLRADVIQGLGIQLLEQVFDLLGEEDELEREARLQEHMGDKYTTYCVKARQLKFFEENVSF

Target ORF information:

RefSeq Version	NM_011849.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 4 (Nek4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011849.3

ORF Insert Sequence:

ATGCCCCAGG CCGCATACTG CTACATGCGG GTCGTGGGCA GGGGTAGTTA CGGGGAGGTG 
ACGCTCGTGA AGCATCGGCG AGACGGCAAG CAGTATGTCA TCAAAAAGCT GAACCTCCGA 
AATGCCTCCA GCCGAGAGCG TCGAGCTGCT GAGCAGGAAG CTCAGCTCCT ATCTCAGTTG 
AAGCACCCCA ACATTGTCAC CTACAAAGAA TCATGGGAGG GAGGAGACGG TCTGCTCTAC 
ATTGTCATGG GCTTCTGCGA AGGAGGTGAT CTGTACCGGA AGCTCAAGGA GCAGAAAGGG 
CAGCTTCTGC CTGAGAGTCA GGTGGTGGAG TGGTTTGTCC AGATCGCCAT GGCCTTGCAG 
TATTTACATG AGAAACACAT CCTTCATCGA GATCTGAAAA CTCAGAATGT CTTCTTAACA 
AGAACAAACA TCATCAAAGT GGGGGACCTA GGAATTGCCC GCGTCTTAGA GAATCACGGT 
GACATGGCGA GCACCCTCAT TGGCACACCC TATTACATGA GCCCTGAGCT GTTCTCCAAC 
AAACCCTACA ACTATAAGTC GGATGTTTGG GCTTTGGGAT GCTGTGTATA TGAAATGGCC 
ACCCTGAAGC ACGCTTTTAA TGCAAAAGAC ATGAATTCTT TAGTTTATCG GATTATTGAA 
GGAAAGTTGC CGCCAATGCC AAAAGTTTAC AGCACAGAGC TGGCTGAGCT GATAAGAACA 
ATGCTGAGCA GAAGGCCTGA GGAGAGGCCC TCTGTGCGGA GCATCCTGAG GCAGCCCTAC 
ATAAAACACC ACATCTCCTT GTTCTTGGAG GCCACCAAGG CAAAAACTTC CAAAAATAAT 
GTTAAAAACT GTGACTCCAG AGCCAAGCCT GTTGCTGCTG TAGTTTCCAG AAAGGAAGAA 
TCAAATACTG ACGTGATACA CTACCAGCCA CGCTCTTCTG AGGGCTCTGC TTTGCATGTC 
ATGGGTGAAG ATAAATGTTT GTCCCAAGAG AAACCAGTGG ACATTGGTCC CTTGAGATCA 
CCGGCTAGTC TTGAAGGCCA CACTGGCAAA CAAGACATGA ACAATACTGG GGAGTCATGT 
GCCACAATCA GTAGGATAAA TATCGATATC TTACCTGCAG AAAGGAGGGA CTCAGCGAAC 
GCTGGTGTAG TTCAGGAGAG CCAACCACAA CACGTGGATG CCGCTGATGA AGTAGACAGT 
CAGTGCAGTA TTTCTCAAGA GAAGGAGAGG CTACAGGGCA ATACCAAGTC CAGTGATCAG 
CCTGGAAATC TCCTTCCCAG ACGGTCCTCT GATGGTGGTG ATGGGGAAGG GAGTGAACTG 
GTGAAGCCAT TGTATCCCTC AAATAAAGAC CAAAAGCCAG ACCAGGATCA AGTTACTGGT 
ATTATAGAAA ATCAGGACAG CATCCACCCA AGATCCCAGC CACACAGCTC TATGTCTGAA 
CCTTCTTTGT CCCGGCAGCG AAGGCAGAAA AAAAGGGAAC AGACTGCCCA TAGTCGTACA 
AAGAGCCAGT TCCAAGAATT ACCCCCTCGG CTTTTGCCTT CTTATCCTGG TATTGGGAAA 
GTGGATATCA TAGCAACACA GCAAAATGAT GGAAACCAAG GTGGACCTGT GGCTGGGTGT 
GTAAACAGTT CAAGGACCAG CTCAACAGCA TCAGCAAAGG ATCGACCATT ATCAGCCAGA 
GAGAGGAGAC GGCTAAAGCA GTCACAGGAG GAGATGCTGC CCTCAGGCCC TGCAGTTCAG 
AGAACTCCAA GTGCAGTTGA GCCACTGAAA CCACAGGAAG AAGACCAACC CATCCCTGCC 
CAACGATTCT CTTCTGACTG CAGCATCACT CAGATGAATC ACACACTCCC GAGGGAAAAG 
GAGAAGAGGC TGATGCATGG CCTGTCTGAG GATGAGTTAA GCTCTTCTAC AAGCTCAACT 
GATAAGTCAG ATGGGGATTC CAGGGAGGGG AAGAGTCATA CAAATGAAAT GAAAGACTTG 
GTACAGCTGA TGACTCAGAC TCTGAGGCTG GAAGCTAAGG AGAGCTGTGA AGATCTCCAG 
GTCCTAAACC CAGGGTCAGA ATTCAGACTT CATCGAAAGT ATCGAGACAC GCTGGTGCTT 
CATGGCAAGG TTGCAGAAGA GGTGGAACCC CACTGTACGG AGCTGCCTAC GGGTATCATA 
CCAGGTTCTG AAAAGATCAG GAGAATCGTT GAAGTCTTAA GAGCTGATGT AATCCAGGGC 
CTGGGGATTC AACTCTTAGA GCAGGTGTTT GATCTTTTGG GAGAGGAAGA TGAGTTGGAG 
AGAGAGGCAC GATTACAGGA GCACATGGGA GACAAGTATA CAACTTACTG TGTGAAAGCT 
CGCCAATTGA AATTTTTTGA AGAAAATGTG AGTTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nek4 ( XM_011245068.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011243370.1 Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 4 (Nek4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu69144
Clone ID Related Accession (Same CDS sequence)	XM_011245068.2 , XM_011245068.3
Accession Version	XM_011245068.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2358bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase Nek4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011245068.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCACC CCACCCAGCC AGTCTCATGC TTTTTGATCA TCCTCCTGCC TCGTCTTCGA 
TGTATTATAC TGTATGTCAT CAAAAAGCTG AACCTCCGAA ATGCCTCCAG CCGAGAGCGT 
CGAGCTGCTG AGCAGGAAGC TCAGCTCCTA TCTCAGTTGA AGCACCCCAA CATTGTCACC 
TACAAAGAAT CATGGGAGGG AGGAGACGGT CTGCTCTACA TTGTCATGGG CTTCTGCGAA 
GGAGGTGATC TGTACCGGAA GCTCAAGGAG CAGAAAGGGC AGCTTCTGCC TGAGAGTCAG 
GTGGTGGAGT GGTTTGTCCA GATCGCCATG GCCTTGCAGT ATTTACATGA GAAACACATC 
CTTCATCGAG ATCTGAAAAC TCAGAATGTC TTCTTAACAA GAACAAACAT CATCAAAGTG 
GGGGACCTAG GAATTGCCCG CGTCTTAGAG AATCACGGTG ACATGGCGAG CACCCTCATT 
GGCACACCCT ATTACATGAG CCCTGAGCTG TTCTCCAACA AACCCTACAA CTATAAGTCG 
GATGTTTGGG CTTTGGGATG CTGTGTATAT GAAATGGCCA CCCTGAAGCA CGCTTTTAAT 
GCAAAAGACA TGAATTCTTT AGTTTATCGG ATTATTGAAG GAAAGTTGCC GCCAATGCCA 
AAAGTTTACA GCACAGAGCT GGCTGAGCTG ATAAGAACAA TGCTGAGCAG AAGGCCTGAG 
GAGAGGCCCT CTGTGCGGAG CATCCTGAGG CAGCCCTACA TAAAACACCA CATCTCCTTG 
TTCTTGGAGG CCACCAAGGC AAAAACTTCC AAAAATAATG TTAAAAACTG TGACTCCAGA 
GCCAAGCCTG TTGCTGCTGT AGTTTCCAGA AAGGAAGAAT CAAATACTGA CGTGATACAC 
TACCAGCCAC GCTCTTCTGA GGGCTCTGCT TTGCATGTCA TGGGTGAAGA TAAATGTTTG 
TCCCAAGAGA AACCAGTGGA CATTGGTCCC TTGAGATCAC CGGCTAGTCT TGAAGGCCAC 
ACTGGCAAAC AAGACATGAA CAATACTGGG GAGTCATGTG CCACAATCAG TAGGATAAAT 
ATCGATATCT TACCTGCAGA AAGGAGGGAC TCAGCGAACG CTGGTGTAGT TCAGGAGAGC 
CAACCACAAC ACGTGGATGC CGCTGATGAA GTAGACAGTC AGTGCAGTAT TTCTCAAGAG 
AAGGAGAGGC TACAGGGCAA TACCAAGTCC AGTGATCAGC CTGGAAATCT CCTTCCCAGA 
CGGTCCTCTG ATGGTGGTGA TGGGGAAGGG AGTGAACTGG TGAAGCCATT GTATCCCTCA 
AATAAAGACC AAAAGCCAGA CCAGGATCAA GTTACTGGTA TTATAGAAAA TCAGGACAGC 
ATCCACCCAA GATCCCAGCC ACACAGCTCT ATGTCTGAAC CTTCTTTGTC CCGGCAGCGA 
AGGCAGAAAA AAAGGGAACA GACTGCCCAT AGTCGTACAA AGAGCCAGTT CCAAGAATTA 
CCCCCTCGGC TTTTGCCTTC TTATCCTGGT ATTGGGAAAG TGGATATCAT AGCAACACAG 
CAAAATGATG GAAACCAAGG TGGACCTGTG GCTGGGTGTG TAAACAGTTC AAGGACCAGC 
TCAACAGCAT CAGCAAAGGA TCGACCATTA TCAGCCAGAG AGAGGAGACG GCTAAAGCAG 
TCACAGGAGG AGATGCTGCC CTCAGGCCCT GCAGTTCAGA GAACTCCAAG TGCAGTTGAG 
CCACTGAAAC CACAGGAAGA AGACCAACCC ATCCCTGCCC AACGATTCTC TTCTGACTGC 
AGCATCACTC AGATGAATCA CACACTCCCG AGGGAAAAGG AGAAGAGGCT GATGCATGGC 
CTGTCTGAGG ATGAGTTAAG CTCTTCTACA AGCTCAACTG ATAAGTCAGA TGGGGATTCC 
AGGGAGGGGA AGAGTCATAC AAATGAAATG AAAGACTTGG TACAGCTGAT GACTCAGACT 
CTGAGGCTGG AAGCTAAGGA GAGCTGTGAA GATCTCCAGG TCCTAAACCC AGGGTCAGAA 
TTCAGACTTC ATCGAAAGTA TCGAGACACG CTGGTGCTTC ATGGCAAGGT TGCAGAAGAG 
GTGGAACCCC ACTGTACGGA GCTGCCTACG GGTATCATAC CAGGTTCTGA AAAGATCAGG 
AGAATCGTTG AAGTCTTAAG AGCTGATGTA ATCCAGGGCC TGGGGATTCA ACTCTTAGAG 
CAGGTGTTTG ATCTTTTGGG AGAGGAAGAT GAGTTGGAGA GAGAGGCACG ATTACAGGAG 
CACATGGGAG ACAAGTATAC AACTTACTGT GTGAAAGCTC GCCAATTGAA ATTTTTTGAA 
GAAAATGTGA GTTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011243370.1
CDS	1169..3526
Translation	MDHPTQPVSCFLIILLPRLRCIILYVIKKLNLRNASSRERRAAEQEAQLLSQLKHPNIVTYKESWEGGDGLLYIVMGFCEGGDLYRKLKEQKGQLLPESQVVEWFVQIAMALQYLHEKHILHRDLKTQNVFLTRTNIIKVGDLGIARVLENHGDMASTLIGTPYYMSPELFSNKPYNYKSDVWALGCCVYEMATLKHAFNAKDMNSLVYRIIEGKLPPMPKVYSTELAELIRTMLSRRPEERPSVRSILRQPYIKHHISLFLEATKAKTSKNNVKNCDSRAKPVAAVVSRKEESNTDVIHYQPRSSEGSALHVMGEDKCLSQEKPVDIGPLRSPASLEGHTGKQDMNNTGESCATISRINIDILPAERRDSANAGVVQESQPQHVDAADEVDSQCSISQEKERLQGNTKSSDQPGNLLPRRSSDGGDGEGSELVKPLYPSNKDQKPDQDQVTGIIENQDSIHPRSQPHSSMSEPSLSRQRRQKKREQTAHSRTKSQFQELPPRLLPSYPGIGKVDIIATQQNDGNQGGPVAGCVNSSRTSSTASAKDRPLSARERRRLKQSQEEMLPSGPAVQRTPSAVEPLKPQEEDQPIPAQRFSSDCSITQMNHTLPREKEKRLMHGLSEDELSSSTSSTDKSDGDSREGKSHTNEMKDLVQLMTQTLRLEAKESCEDLQVLNPGSEFRLHRKYRDTLVLHGKVAEEVEPHCTELPTGIIPGSEKIRRIVEVLRADVIQGLGIQLLEQVFDLLGEEDELEREARLQEHMGDKYTTYCVKARQLKFFEENVSF

Target ORF information:

RefSeq Version	XM_011245068.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 4 (Nek4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011245068.3

ORF Insert Sequence:

ATGGACCACC CCACCCAGCC AGTCTCATGC TTTTTGATCA TCCTCCTGCC TCGTCTTCGA 
TGTATTATAC TGTATGTCAT CAAAAAGCTG AACCTCCGAA ATGCCTCCAG CCGAGAGCGT 
CGAGCTGCTG AGCAGGAAGC TCAGCTCCTA TCTCAGTTGA AGCACCCCAA CATTGTCACC 
TACAAAGAAT CATGGGAGGG AGGAGACGGT CTGCTCTACA TTGTCATGGG CTTCTGCGAA 
GGAGGTGATC TGTACCGGAA GCTCAAGGAG CAGAAAGGGC AGCTTCTGCC TGAGAGTCAG 
GTGGTGGAGT GGTTTGTCCA GATCGCCATG GCCTTGCAGT ATTTACATGA GAAACACATC 
CTTCATCGAG ATCTGAAAAC TCAGAATGTC TTCTTAACAA GAACAAACAT CATCAAAGTG 
GGGGACCTAG GAATTGCCCG CGTCTTAGAG AATCACGGTG ACATGGCGAG CACCCTCATT 
GGCACACCCT ATTACATGAG CCCTGAGCTG TTCTCCAACA AACCCTACAA CTATAAGTCG 
GATGTTTGGG CTTTGGGATG CTGTGTATAT GAAATGGCCA CCCTGAAGCA CGCTTTTAAT 
GCAAAAGACA TGAATTCTTT AGTTTATCGG ATTATTGAAG GAAAGTTGCC GCCAATGCCA 
AAAGTTTACA GCACAGAGCT GGCTGAGCTG ATAAGAACAA TGCTGAGCAG AAGGCCTGAG 
GAGAGGCCCT CTGTGCGGAG CATCCTGAGG CAGCCCTACA TAAAACACCA CATCTCCTTG 
TTCTTGGAGG CCACCAAGGC AAAAACTTCC AAAAATAATG TTAAAAACTG TGACTCCAGA 
GCCAAGCCTG TTGCTGCTGT AGTTTCCAGA AAGGAAGAAT CAAATACTGA CGTGATACAC 
TACCAGCCAC GCTCTTCTGA GGGCTCTGCT TTGCATGTCA TGGGTGAAGA TAAATGTTTG 
TCCCAAGAGA AACCAGTGGA CATTGGTCCC TTGAGATCAC CGGCTAGTCT TGAAGGCCAC 
ACTGGCAAAC AAGACATGAA CAATACTGGG GAGTCATGTG CCACAATCAG TAGGATAAAT 
ATCGATATCT TACCTGCAGA AAGGAGGGAC TCAGCGAACG CTGGTGTAGT TCAGGAGAGC 
CAACCACAAC ACGTGGATGC CGCTGATGAA GTAGACAGTC AGTGCAGTAT TTCTCAAGAG 
AAGGAGAGGC TACAGGGCAA TACCAAGTCC AGTGATCAGC CTGGAAATCT CCTTCCCAGA 
CGGTCCTCTG ATGGTGGTGA TGGGGAAGGG AGTGAACTGG TGAAGCCATT GTATCCCTCA 
AATAAAGACC AAAAGCCAGA CCAGGATCAA GTTACTGGTA TTATAGAAAA TCAGGACAGC 
ATCCACCCAA GATCCCAGCC ACACAGCTCT ATGTCTGAAC CTTCTTTGTC CCGGCAGCGA 
AGGCAGAAAA AAAGGGAACA GACTGCCCAT AGTCGTACAA AGAGCCAGTT CCAAGAATTA 
CCCCCTCGGC TTTTGCCTTC TTATCCTGGT ATTGGGAAAG TGGATATCAT AGCAACACAG 
CAAAATGATG GAAACCAAGG TGGACCTGTG GCTGGGTGTG TAAACAGTTC AAGGACCAGC 
TCAACAGCAT CAGCAAAGGA TCGACCATTA TCAGCCAGAG AGAGGAGACG GCTAAAGCAG 
TCACAGGAGG AGATGCTGCC CTCAGGCCCT GCAGTTCAGA GAACTCCAAG TGCAGTTGAG 
CCACTGAAAC CACAGGAAGA AGACCAACCC ATCCCTGCCC AACGATTCTC TTCTGACTGC 
AGCATCACTC AGATGAATCA CACACTCCCG AGGGAAAAGG AGAAGAGGCT GATGCATGGC 
CTGTCTGAGG ATGAGTTAAG CTCTTCTACA AGCTCAACTG ATAAGTCAGA TGGGGATTCC 
AGGGAGGGGA AGAGTCATAC AAATGAAATG AAAGACTTGG TACAGCTGAT GACTCAGACT 
CTGAGGCTGG AAGCTAAGGA GAGCTGTGAA GATCTCCAGG TCCTAAACCC AGGGTCAGAA 
TTCAGACTTC ATCGAAAGTA TCGAGACACG CTGGTGCTTC ATGGCAAGGT TGCAGAAGAG 
GTGGAACCCC ACTGTACGGA GCTGCCTACG GGTATCATAC CAGGTTCTGA AAAGATCAGG 
AGAATCGTTG AAGTCTTAAG AGCTGATGTA ATCCAGGGCC TGGGGATTCA ACTCTTAGAG 
CAGGTGTTTG ATCTTTTGGG AGAGGAAGAT GAGTTGGAGA GAGAGGCACG ATTACAGGAG 
CACATGGGAG ACAAGTATAC AACTTACTGT GTGAAAGCTC GCCAATTGAA ATTTTTTGAA 
GAAAATGTGA GTTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nek4 ( XM_011245069.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011243371.1 Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 4 (Nek4), transcript variant X2, mRNA.

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CloneID	OMu69145
Clone ID Related Accession (Same CDS sequence)	XM_011245069.2 , XM_011245069.3
Accession Version	XM_011245069.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2133bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase Nek4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011245069.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTTCT GCGAAGGAGG TGATCTGTAC CGGAAGCTCA AGGAGCAGAA AGGGCAGCTT 
CTGCCTGAGA GTCAGGTGGT GGAGTGGTTT GTCCAGATCG CCATGGCCTT GCAGTATTTA 
CATGAGAAAC ACATCCTTCA TCGAGATCTG AAAACTCAGA ATGTCTTCTT AACAAGAACA 
AACATCATCA AAGTGGGGGA CCTAGGAATT GCCCGCGTCT TAGAGAATCA CGGTGACATG 
GCGAGCACCC TCATTGGCAC ACCCTATTAC ATGAGCCCTG AGCTGTTCTC CAACAAACCC 
TACAACTATA AGTCGGATGT TTGGGCTTTG GGATGCTGTG TATATGAAAT GGCCACCCTG 
AAGCACGCTT TTAATGCAAA AGACATGAAT TCTTTAGTTT ATCGGATTAT TGAAGGAAAG 
TTGCCGCCAA TGCCAAAAGT TTACAGCACA GAGCTGGCTG AGCTGATAAG AACAATGCTG 
AGCAGAAGGC CTGAGGAGAG GCCCTCTGTG CGGAGCATCC TGAGGCAGCC CTACATAAAA 
CACCACATCT CCTTGTTCTT GGAGGCCACC AAGGCAAAAA CTTCCAAAAA TAATGTTAAA 
AACTGTGACT CCAGAGCCAA GCCTGTTGCT GCTGTAGTTT CCAGAAAGGA AGAATCAAAT 
ACTGACGTGA TACACTACCA GCCACGCTCT TCTGAGGGCT CTGCTTTGCA TGTCATGGGT 
GAAGATAAAT GTTTGTCCCA AGAGAAACCA GTGGACATTG GTCCCTTGAG ATCACCGGCT 
AGTCTTGAAG GCCACACTGG CAAACAAGAC ATGAACAATA CTGGGGAGTC ATGTGCCACA 
ATCAGTAGGA TAAATATCGA TATCTTACCT GCAGAAAGGA GGGACTCAGC GAACGCTGGT 
GTAGTTCAGG AGAGCCAACC ACAACACGTG GATGCCGCTG ATGAAGTAGA CAGTCAGTGC 
AGTATTTCTC AAGAGAAGGA GAGGCTACAG GGCAATACCA AGTCCAGTGA TCAGCCTGGA 
AATCTCCTTC CCAGACGGTC CTCTGATGGT GGTGATGGGG AAGGGAGTGA ACTGGTGAAG 
CCATTGTATC CCTCAAATAA AGACCAAAAG CCAGACCAGG ATCAAGTTAC TGGTATTATA 
GAAAATCAGG ACAGCATCCA CCCAAGATCC CAGCCACACA GCTCTATGTC TGAACCTTCT 
TTGTCCCGGC AGCGAAGGCA GAAAAAAAGG GAACAGACTG CCCATAGTCG TACAAAGAGC 
CAGTTCCAAG AATTACCCCC TCGGCTTTTG CCTTCTTATC CTGGTATTGG GAAAGTGGAT 
ATCATAGCAA CACAGCAAAA TGATGGAAAC CAAGGTGGAC CTGTGGCTGG GTGTGTAAAC 
AGTTCAAGGA CCAGCTCAAC AGCATCAGCA AAGGATCGAC CATTATCAGC CAGAGAGAGG 
AGACGGCTAA AGCAGTCACA GGAGGAGATG CTGCCCTCAG GCCCTGCAGT TCAGAGAACT 
CCAAGTGCAG TTGAGCCACT GAAACCACAG GAAGAAGACC AACCCATCCC TGCCCAACGA 
TTCTCTTCTG ACTGCAGCAT CACTCAGATG AATCACACAC TCCCGAGGGA AAAGGAGAAG 
AGGCTGATGC ATGGCCTGTC TGAGGATGAG TTAAGCTCTT CTACAAGCTC AACTGATAAG 
TCAGATGGGG ATTCCAGGGA GGGGAAGAGT CATACAAATG AAATGAAAGA CTTGGTACAG 
CTGATGACTC AGACTCTGAG GCTGGAAGCT AAGGAGAGCT GTGAAGATCT CCAGGTCCTA 
AACCCAGGGT CAGAATTCAG ACTTCATCGA AAGTATCGAG ACACGCTGGT GCTTCATGGC 
AAGGTTGCAG AAGAGGTGGA ACCCCACTGT ACGGAGCTGC CTACGGGTAT CATACCAGGT 
TCTGAAAAGA TCAGGAGAAT CGTTGAAGTC TTAAGAGCTG ATGTAATCCA GGGCCTGGGG 
ATTCAACTCT TAGAGCAGGT GTTTGATCTT TTGGGAGAGG AAGATGAGTT GGAGAGAGAG 
GCACGATTAC AGGAGCACAT GGGAGACAAG TATACAACTT ACTGTGTGAA AGCTCGCCAA 
TTGAAATTTT TTGAAGAAAA TGTGAGTTTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011243371.1
CDS	539..2671
Translation	MGFCEGGDLYRKLKEQKGQLLPESQVVEWFVQIAMALQYLHEKHILHRDLKTQNVFLTRTNIIKVGDLGIARVLENHGDMASTLIGTPYYMSPELFSNKPYNYKSDVWALGCCVYEMATLKHAFNAKDMNSLVYRIIEGKLPPMPKVYSTELAELIRTMLSRRPEERPSVRSILRQPYIKHHISLFLEATKAKTSKNNVKNCDSRAKPVAAVVSRKEESNTDVIHYQPRSSEGSALHVMGEDKCLSQEKPVDIGPLRSPASLEGHTGKQDMNNTGESCATISRINIDILPAERRDSANAGVVQESQPQHVDAADEVDSQCSISQEKERLQGNTKSSDQPGNLLPRRSSDGGDGEGSELVKPLYPSNKDQKPDQDQVTGIIENQDSIHPRSQPHSSMSEPSLSRQRRQKKREQTAHSRTKSQFQELPPRLLPSYPGIGKVDIIATQQNDGNQGGPVAGCVNSSRTSSTASAKDRPLSARERRRLKQSQEEMLPSGPAVQRTPSAVEPLKPQEEDQPIPAQRFSSDCSITQMNHTLPREKEKRLMHGLSEDELSSSTSSTDKSDGDSREGKSHTNEMKDLVQLMTQTLRLEAKESCEDLQVLNPGSEFRLHRKYRDTLVLHGKVAEEVEPHCTELPTGIIPGSEKIRRIVEVLRADVIQGLGIQLLEQVFDLLGEEDELEREARLQEHMGDKYTTYCVKARQLKFFEENVSF

Target ORF information:

RefSeq Version	XM_011245069.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 4 (Nek4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011245069.3

ORF Insert Sequence:

ATGGGCTTCT GCGAAGGAGG TGATCTGTAC CGGAAGCTCA AGGAGCAGAA AGGGCAGCTT 
CTGCCTGAGA GTCAGGTGGT GGAGTGGTTT GTCCAGATCG CCATGGCCTT GCAGTATTTA 
CATGAGAAAC ACATCCTTCA TCGAGATCTG AAAACTCAGA ATGTCTTCTT AACAAGAACA 
AACATCATCA AAGTGGGGGA CCTAGGAATT GCCCGCGTCT TAGAGAATCA CGGTGACATG 
GCGAGCACCC TCATTGGCAC ACCCTATTAC ATGAGCCCTG AGCTGTTCTC CAACAAACCC 
TACAACTATA AGTCGGATGT TTGGGCTTTG GGATGCTGTG TATATGAAAT GGCCACCCTG 
AAGCACGCTT TTAATGCAAA AGACATGAAT TCTTTAGTTT ATCGGATTAT TGAAGGAAAG 
TTGCCGCCAA TGCCAAAAGT TTACAGCACA GAGCTGGCTG AGCTGATAAG AACAATGCTG 
AGCAGAAGGC CTGAGGAGAG GCCCTCTGTG CGGAGCATCC TGAGGCAGCC CTACATAAAA 
CACCACATCT CCTTGTTCTT GGAGGCCACC AAGGCAAAAA CTTCCAAAAA TAATGTTAAA 
AACTGTGACT CCAGAGCCAA GCCTGTTGCT GCTGTAGTTT CCAGAAAGGA AGAATCAAAT 
ACTGACGTGA TACACTACCA GCCACGCTCT TCTGAGGGCT CTGCTTTGCA TGTCATGGGT 
GAAGATAAAT GTTTGTCCCA AGAGAAACCA GTGGACATTG GTCCCTTGAG ATCACCGGCT 
AGTCTTGAAG GCCACACTGG CAAACAAGAC ATGAACAATA CTGGGGAGTC ATGTGCCACA 
ATCAGTAGGA TAAATATCGA TATCTTACCT GCAGAAAGGA GGGACTCAGC GAACGCTGGT 
GTAGTTCAGG AGAGCCAACC ACAACACGTG GATGCCGCTG ATGAAGTAGA CAGTCAGTGC 
AGTATTTCTC AAGAGAAGGA GAGGCTACAG GGCAATACCA AGTCCAGTGA TCAGCCTGGA 
AATCTCCTTC CCAGACGGTC CTCTGATGGT GGTGATGGGG AAGGGAGTGA ACTGGTGAAG 
CCATTGTATC CCTCAAATAA AGACCAAAAG CCAGACCAGG ATCAAGTTAC TGGTATTATA 
GAAAATCAGG ACAGCATCCA CCCAAGATCC CAGCCACACA GCTCTATGTC TGAACCTTCT 
TTGTCCCGGC AGCGAAGGCA GAAAAAAAGG GAACAGACTG CCCATAGTCG TACAAAGAGC 
CAGTTCCAAG AATTACCCCC TCGGCTTTTG CCTTCTTATC CTGGTATTGG GAAAGTGGAT 
ATCATAGCAA CACAGCAAAA TGATGGAAAC CAAGGTGGAC CTGTGGCTGG GTGTGTAAAC 
AGTTCAAGGA CCAGCTCAAC AGCATCAGCA AAGGATCGAC CATTATCAGC CAGAGAGAGG 
AGACGGCTAA AGCAGTCACA GGAGGAGATG CTGCCCTCAG GCCCTGCAGT TCAGAGAACT 
CCAAGTGCAG TTGAGCCACT GAAACCACAG GAAGAAGACC AACCCATCCC TGCCCAACGA 
TTCTCTTCTG ACTGCAGCAT CACTCAGATG AATCACACAC TCCCGAGGGA AAAGGAGAAG 
AGGCTGATGC ATGGCCTGTC TGAGGATGAG TTAAGCTCTT CTACAAGCTC AACTGATAAG 
TCAGATGGGG ATTCCAGGGA GGGGAAGAGT CATACAAATG AAATGAAAGA CTTGGTACAG 
CTGATGACTC AGACTCTGAG GCTGGAAGCT AAGGAGAGCT GTGAAGATCT CCAGGTCCTA 
AACCCAGGGT CAGAATTCAG ACTTCATCGA AAGTATCGAG ACACGCTGGT GCTTCATGGC 
AAGGTTGCAG AAGAGGTGGA ACCCCACTGT ACGGAGCTGC CTACGGGTAT CATACCAGGT 
TCTGAAAAGA TCAGGAGAAT CGTTGAAGTC TTAAGAGCTG ATGTAATCCA GGGCCTGGGG 
ATTCAACTCT TAGAGCAGGT GTTTGATCTT TTGGGAGAGG AAGATGAGTT GGAGAGAGAG 
GCACGATTAC AGGAGCACAT GGGAGACAAG TATACAACTT ACTGTGTGAA AGCTCGCCAA 
TTGAAATTTT TTGAAGAAAA TGTGAGTTTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Nek4 status differentially alters sensitivity to distinct microtubule poisons.
Cancer research70(3)1033-41(2010 Feb)
Doles J,Hemann MT

Construction of a multi-functional cDNA library specific for mouse pancreatic islets and its application to microarray.
DNA research : an international journal for rapid publication of reports on genes and genomes11(5)315-23(2004 Oct)
Nishimura M,Yokoi N,Miki T,Horikawa Y,Yoshioka H,Takeda J,Ohara O,Seino S

Exploration of the cell-cycle genes found within the RIKEN FANTOM2 data set.
Genome research13(6B)1366-75(2003 Jun)
Forrest AR,Taylor D,Grimmond S,,

Activity and substrate specificity of the murine STK2 Serine/Threonine kinase that is structurally related to the mitotic regulator protein NIMA of Aspergillus nidulans.
Biochemical and biophysical research communications264(2)449-56(1999 Oct)
Hayashi K,Igarashi H,Ogawa M,Sakaguchi N

NIMA-related kinases: isolation and characterization of murine nek3 and nek4 cDNAs, and chromosomal localization of nek1, nek2 and nek3.
Gene234(1)127-37(1999 Jun)
Chen A,Yanai A,Arama E,Kilfin G,Motro B

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Nek4 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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