Hax1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hax1
Entrez Gene ID	23897
Full Name	HCLS1 associated X-1
Synonyms	HAX-1,HSP1BP-1,Hs1bp1,SIG-111,Silg111,mHAX-1s
General protein information	Preferred Names HCLS1 associated X-1 Names HCLS1-associated protein X-1 HS1 binding protein HS1-associated protein X-1 HS1-associating protein X-1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 3 Map Location: 3\|3 F1

mRNA and Protein(s)

mRNA	Protein	Name
XM_006501446.2	XP_006501509.1	HCLS1-associated protein X-1 isoform X2
NM_001282032.1	NP_001268961.1	HCLS1-associated protein X-1 isoform 2
NM_011826.4	NP_035956.1	HCLS1-associated protein X-1 isoform 1
XM_017319570.1	XP_017175059.1	HCLS1-associated protein X-1 isoform X2
XM_006501446.3	XP_006501509.1	HCLS1-associated protein X-1 isoform X1
XM_017319569.1	XP_017175058.1	HCLS1-associated protein X-1 isoform X1
NM_001310681.1	NP_001297610.1	HCLS1-associated protein X-1 isoform 3
XM_017319570.2	XP_017175059.1	HCLS1-associated protein X-1 isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	HAX1	NP_001039418.2
Danio rerio (zebrafish)	hax1	NP_001002337.1
	HAX1	XP_002801842.1
Pan troglodytes (chimpanzee)	HAX1	XP_001150932.1
Canis lupus familiaris (dog)	HAX1	XP_005622847.1
Mus musculus (house mouse)	Hax1	NP_035956.1
Xenopus tropicalis (tropical clawed frog)	LOC100485912	XP_002943156.2
Homo sapiens (human)	HAX1	NP_006109.2
Rattus norvegicus (Norway rat)	Hax1	NP_853658.1

Gene Ontology
Bibliography

Related articles in PubMed

The antiapoptotic protein HAX-1 mediates half of phospholamban's inhibitory activity on calcium cycling and contractility in the heart.
Bidwell PA, Haghighi K, Kranias EG
The Journal of biological chemistry293(1)359-367(2018 Jan)

HAX-1 regulates SERCA2a oxidation and degradation.
Bidwell PA, Liu GS, Nagarajan N, Lam CK, Haghighi K, Gardner G, Cai WF, Zhao W, Mugge L, Vafiadaki E, Sanoudou D, Rubinstein J, Lebeche D, Hajjar R, Sadoshima J, Kranias EG
Journal of molecular and cellular cardiology114220-233(2017 Nov)

Mitochondrial Omi/HtrA2 Promotes Caspase Activation Through Cleavage of HAX-1 in Aging Heart.
Liu X, Lei J, Wang K, Ma L, Liu D, Du Y, Wu Y, Zhang S, Wang W, Ma X, Liu H
Rejuvenation research20(3)183-192(2017 Jun)

MLF1 is a proapoptotic antagonist of HOP complex-mediated survival.
Sun Y, Chao JR, Xu W, Pourpak A, Boyd K, Moshiach S, Qi GY, Fu A, Shao HR, Pounds S, Morris SW
Biochimica et biophysica acta1864(4)719-727(2017 04)

Kv3.3 Channels Bind Hax-1 and Arp2/3 to Assemble a Stable Local Actin Network that Regulates Channel Gating.
Zhang Y, Zhang XF, Fleming MR, Amiri A, El-Hassar L, Surguchev AA, Hyland C, Jenkins DP, Desai R, Brown MR, Gazula VR, Waters MF, Large CH, Horvath TL, Navaratnam D, Vaccarino FM, Forscher P, Kaczmarek LK
Cell165(2)434-448(2016 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

HAX1 plays a general role in B cell receptor-mediated internalization events and BCR-mediated apoptosis.
Title: HAX1 deletion impairs BCR internalization and leads to delayed BCR-mediated apoptosis.

The hydrophilic sequences within loop 2, and the matrix-localized hydrophilic domain of UCP3, were necessary for binding to Hax-1 at the C-terminal domain, adjacent to the mitochondrial inner membrane.
Title: UCP3 is associated with Hax-1 in mitochondria in the presence of calcium ion.

Knockdown of HAX1 and EB2 in skin epidermal cells stabilizes focal adhesions and impairs epidermal migration.
Title: Regulation of Focal Adhesion Dynamics and Cell Motility by the EB2 and Hax1 Protein Complex.

These findings reveal the role of HAX-1 in regulating cyclophilin-D levels via an Hsp90-dependent mechanism, resulting in protection against activation of mPTP and subsequent cell death responses.
Title: HAX-1 regulates cyclophilin-D levels and mitochondria permeability transition pore in the heart.

Protease Omi impairs mitochondrial function by cleaving Hax-1, which induces apoptosis in oxygen-glucose deprivation and reoxygenation -treated N2a cells and causes reperfusion injury in middle cerebral artery occlusion mice.
Title: Protease Omi cleaving Hax-1 protein contributes to OGD/R-induced mitochondrial damage in neuroblastoma N2a cells and cerebral injury in MCAO mice.

The following Hax1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hax1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu08937	NM_011826.4 Latest version!	Mus musculus HCLS1 associated X-1 (Hax1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu31889	NM_001310681.1 Latest version!	Mus musculus HCLS1 associated X-1 (Hax1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu08393	NM_001282032.1 Latest version!	Mus musculus HCLS1 associated X-1 (Hax1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu31889	XM_006501446.3 Latest version!	Mus musculus HCLS1 associated X-1 (Hax1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu31889	XM_017319570.2 Latest version!	Mus musculus HCLS1 associated X-1 (Hax1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu80614	XM_017319569.1 Latest version!	Mus musculus HCLS1 associated X-1 (Hax1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OMu31889	XM_006501446.2	Mus musculus HCLS1 associated X-1 (Hax1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu31889	XM_017319570.1	Mus musculus HCLS1 associated X-1 (Hax1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hax1 ( NM_011826.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035956.1 Mus musculus HCLS1 associated X-1 (Hax1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu08937
Clone ID Related Accession (Same CDS sequence)	NM_011826.4
Accession Version	NM_011826.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 843bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	HCLS1-associated protein X-1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC163616.3, BY253818.1, BC147481.1 and BM900159.1. On Aug 15, 2013 this sequence version replaced NM_011826.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK010633.1, AK088746.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCGTCT TTGATCTTTT CCGAGGCTTT TTCGGCTTTC CTGGACCTCG GAGCCACAGA 
GATCCTTTTT TTGGAGGGAT GACTCGAGAT GATGACGACG ATGATGATGA TGATGACGAA 
GCGGAGGAAG ACAGAGGCGC GTGGGGTCGA GAGAGCTATG CGTTTGATGG TTCTCAGCCT 
CCAGAGGAAT TCGGTTTCAG CTTCAGCCCC AGGGGAGGGA TGCGATTCCA CGGCAACTTT 
GGCTTTGATG ATCTAGTACG AGATTTTAAT AGCATCTTCA GCGAGATGGG GGCCTGGACC 
TTGCCTTCCC ACTCTCCTGA ACTTCCAGGT CCTGAGTCAG AAACACCTGG TGAGAGACTG 
CGGGAGGGGC AGACACTACG AGACTCAATG CTTAAGTACC CAGATAGTCA CCAACCCAGG 
ATCTTTGAGG GGGTCTTGGA GAGTCATGCA AAACCTGAAT CCCCAAAACC AGCTCCAGAT 
TGGGGGTCGC AGGGACCTTT TCATAGGTTG GATGATACAT GGCCTGTGAG TCCCCATTCT 
AGAGCCAAAG AGGACAAAGA TCTTGACTCC CAGGTTTCCC AGGAAGGTCT GGGTCCACTT 
CTTCAACCCC AGCCCAAATC GTATTTCAAA AGTATCTCTG TGACCAAGAT CACTAAGCCA 
GATGGGACAG TGGAGGAACG CCGCACTGTG GTAGACAGTG AGGGCCGGAG GGAGACCACA 
GTGACCCATC AAGAAGCCCA TGACAGTTCC AGAAGTGATC CAGACTCTCA GAGATCTTCA 
GCTTTGGATG ATCCCTTTTC CATTCTGGAT TTGCTACTAG GACGTTGGTT TCGGTCCCGA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035956.1
CDS	137..979
Translation	MSVFDLFRGFFGFPGPRSHRDPFFGGMTRDDDDDDDDDDEAEEDRGAWGRESYAFDGSQPPEEFGFSFSPRGGMRFHGNFGFDDLVRDFNSIFSEMGAWTLPSHSPELPGPESETPGERLREGQTLRDSMLKYPDSHQPRIFEGVLESHAKPESPKPAPDWGSQGPFHRLDDTWPVSPHSRAKEDKDLDSQVSQEGLGPLLQPQPKSYFKSISVTKITKPDGTVEERRTVVDSEGRRETTVTHQEAHDSSRSDPDSQRSSALDDPFSILDLLLGRWFRSR

Target ORF information:

RefSeq Version	NM_011826.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus HCLS1 associated X-1 (Hax1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011826.4

ORF Insert Sequence:

ATGAGCGTCT TTGATCTTTT CCGAGGCTTT TTCGGCTTTC CTGGACCTCG GAGCCACAGA 
GATCCTTTTT TTGGAGGGAT GACTCGAGAT GATGACGACG ATGATGATGA TGATGACGAA 
GCGGAGGAAG ACAGAGGCGC GTGGGGTCGA GAGAGCTATG CGTTTGATGG TTCTCAGCCT 
CCAGAGGAAT TCGGTTTCAG CTTCAGCCCC AGGGGAGGGA TGCGATTCCA CGGCAACTTT 
GGCTTTGATG ATCTAGTACG AGATTTTAAT AGCATCTTCA GCGAGATGGG GGCCTGGACC 
TTGCCTTCCC ACTCTCCTGA ACTTCCAGGT CCTGAGTCAG AAACACCTGG TGAGAGACTG 
CGGGAGGGGC AGACACTACG AGACTCAATG CTTAAGTACC CAGATAGTCA CCAACCCAGG 
ATCTTTGAGG GGGTCTTGGA GAGTCATGCA AAACCTGAAT CCCCAAAACC AGCTCCAGAT 
TGGGGGTCGC AGGGACCTTT TCATAGGTTG GATGATACAT GGCCTGTGAG TCCCCATTCT 
AGAGCCAAAG AGGACAAAGA TCTTGACTCC CAGGTTTCCC AGGAAGGTCT GGGTCCACTT 
CTTCAACCCC AGCCCAAATC GTATTTCAAA AGTATCTCTG TGACCAAGAT CACTAAGCCA 
GATGGGACAG TGGAGGAACG CCGCACTGTG GTAGACAGTG AGGGCCGGAG GGAGACCACA 
GTGACCCATC AAGAAGCCCA TGACAGTTCC AGAAGTGATC CAGACTCTCA GAGATCTTCA 
GCTTTGGATG ATCCCTTTTC CATTCTGGAT TTGCTACTAG GACGTTGGTT TCGGTCCCGA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hax1 ( NM_001310681.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001297610.1 Mus musculus HCLS1 associated X-1 (Hax1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu31889
Clone ID Related Accession (Same CDS sequence)	XM_006501446.2 , XM_017319570.1 , XM_006501446.3 , NM_001310681.1 , XM_017319570.2
Accession Version	NM_001310681.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 765bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-29
Organism	Mus musculus(house mouse)
Product	HCLS1-associated protein X-1 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DV048999.1, CJ048771.1, AA874206.1 and BM900159.1. On Jul 17, 2015 this sequence version replaced XM_006501447.2. Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start site compared to variant 1. The encoded isoform (3) has a shorter N-terminus than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660821.179167.1, AI663871.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACTCGAG ATGATGACGA CGATGATGAT GATGATGACG AAGCGGAGGA AGACAGAGGC 
GCGTGGGGTC GAGAGAGCTA TGCGTTTGAT GGTTCTCAGC CTCCAGAGGA ATTCGGTTTC 
AGCTTCAGCC CCAGGGGAGG GATGCGATTC CACGGCAACT TTGGCTTTGA TGATCTAGTA 
CGAGATTTTA ATAGCATCTT CAGCGAGATG GGGGCCTGGA CCTTGCCTTC CCACTCTCCT 
GAACTTCCAG GTCCTGAGTC AGAAACACCT GGTGAGAGAC TGCGGGAGGG GCAGACACTA 
CGAGACTCAA TGCTTAAGTA CCCAGATAGT CACCAACCCA GGATCTTTGA GGGGGTCTTG 
GAGAGTCATG CAAAACCTGA ATCCCCAAAA CCAGCTCCAG ATTGGGGGTC GCAGGGACCT 
TTTCATAGGT TGGATGATAC ATGGCCTGTG AGTCCCCATT CTAGAGCCAA AGAGGACAAA 
GATCTTGACT CCCAGGTTTC CCAGGAAGGT CTGGGTCCAC TTCTTCAACC CCAGCCCAAA 
TCGTATTTCA AAAGTATCTC TGTGACCAAG ATCACTAAGC CAGATGGGAC AGTGGAGGAA 
CGCCGCACTG TGGTAGACAG TGAGGGCCGG AGGGAGACCA CAGTGACCCA TCAAGAAGCC 
CATGACAGTT CCAGAAGTGA TCCAGACTCT CAGAGATCTT CAGCTTTGGA TGATCCCTTT 
TCCATTCTGG ATTTGCTACT AGGACGTTGG TTTCGGTCCC GATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001297610.1
CDS	126..890
Translation	MTRDDDDDDDDDDEAEEDRGAWGRESYAFDGSQPPEEFGFSFSPRGGMRFHGNFGFDDLVRDFNSIFSEMGAWTLPSHSPELPGPESETPGERLREGQTLRDSMLKYPDSHQPRIFEGVLESHAKPESPKPAPDWGSQGPFHRLDDTWPVSPHSRAKEDKDLDSQVSQEGLGPLLQPQPKSYFKSISVTKITKPDGTVEERRTVVDSEGRRETTVTHQEAHDSSRSDPDSQRSSALDDPFSILDLLLGRWFRSR

Target ORF information:

RefSeq Version	NM_001310681.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus HCLS1 associated X-1 (Hax1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001310681.1

ORF Insert Sequence:

ATGACTCGAG ATGATGACGA CGATGATGAT GATGATGACG AAGCGGAGGA AGACAGAGGC 
GCGTGGGGTC GAGAGAGCTA TGCGTTTGAT GGTTCTCAGC CTCCAGAGGA ATTCGGTTTC 
AGCTTCAGCC CCAGGGGAGG GATGCGATTC CACGGCAACT TTGGCTTTGA TGATCTAGTA 
CGAGATTTTA ATAGCATCTT CAGCGAGATG GGGGCCTGGA CCTTGCCTTC CCACTCTCCT 
GAACTTCCAG GTCCTGAGTC AGAAACACCT GGTGAGAGAC TGCGGGAGGG GCAGACACTA 
CGAGACTCAA TGCTTAAGTA CCCAGATAGT CACCAACCCA GGATCTTTGA GGGGGTCTTG 
GAGAGTCATG CAAAACCTGA ATCCCCAAAA CCAGCTCCAG ATTGGGGGTC GCAGGGACCT 
TTTCATAGGT TGGATGATAC ATGGCCTGTG AGTCCCCATT CTAGAGCCAA AGAGGACAAA 
GATCTTGACT CCCAGGTTTC CCAGGAAGGT CTGGGTCCAC TTCTTCAACC CCAGCCCAAA 
TCGTATTTCA AAAGTATCTC TGTGACCAAG ATCACTAAGC CAGATGGGAC AGTGGAGGAA 
CGCCGCACTG TGGTAGACAG TGAGGGCCGG AGGGAGACCA CAGTGACCCA TCAAGAAGCC 
CATGACAGTT CCAGAAGTGA TCCAGACTCT CAGAGATCTT CAGCTTTGGA TGATCCCTTT 
TCCATTCTGG ATTTGCTACT AGGACGTTGG TTTCGGTCCC GATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hax1 ( XM_006501446.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501509.1 Mus musculus HCLS1 associated X-1 (Hax1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu31889
Clone ID Related Accession (Same CDS sequence)	XM_006501446.2 , XM_017319570.1 , XM_006501446.3 , NM_001310681.1 , XM_017319570.2
Accession Version	XM_006501446.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 765bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	HCLS1-associated protein X-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006501446.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTCGAG ATGATGACGA CGATGATGAT GATGATGACG AAGCGGAGGA AGACAGAGGC 
GCGTGGGGTC GAGAGAGCTA TGCGTTTGAT GGTTCTCAGC CTCCAGAGGA ATTCGGTTTC 
AGCTTCAGCC CCAGGGGAGG GATGCGATTC CACGGCAACT TTGGCTTTGA TGATCTAGTA 
CGAGATTTTA ATAGCATCTT CAGCGAGATG GGGGCCTGGA CCTTGCCTTC CCACTCTCCT 
GAACTTCCAG GTCCTGAGTC AGAAACACCT GGTGAGAGAC TGCGGGAGGG GCAGACACTA 
CGAGACTCAA TGCTTAAGTA CCCAGATAGT CACCAACCCA GGATCTTTGA GGGGGTCTTG 
GAGAGTCATG CAAAACCTGA ATCCCCAAAA CCAGCTCCAG ATTGGGGGTC GCAGGGACCT 
TTTCATAGGT TGGATGATAC ATGGCCTGTG AGTCCCCATT CTAGAGCCAA AGAGGACAAA 
GATCTTGACT CCCAGGTTTC CCAGGAAGGT CTGGGTCCAC TTCTTCAACC CCAGCCCAAA 
TCGTATTTCA AAAGTATCTC TGTGACCAAG ATCACTAAGC CAGATGGGAC AGTGGAGGAA 
CGCCGCACTG TGGTAGACAG TGAGGGCCGG AGGGAGACCA CAGTGACCCA TCAAGAAGCC 
CATGACAGTT CCAGAAGTGA TCCAGACTCT CAGAGATCTT CAGCTTTGGA TGATCCCTTT 
TCCATTCTGG ATTTGCTACT AGGACGTTGG TTTCGGTCCC GATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501509.1
CDS	279..1043
Translation	MTRDDDDDDDDDDEAEEDRGAWGRESYAFDGSQPPEEFGFSFSPRGGMRFHGNFGFDDLVRDFNSIFSEMGAWTLPSHSPELPGPESETPGERLREGQTLRDSMLKYPDSHQPRIFEGVLESHAKPESPKPAPDWGSQGPFHRLDDTWPVSPHSRAKEDKDLDSQVSQEGLGPLLQPQPKSYFKSISVTKITKPDGTVEERRTVVDSEGRRETTVTHQEAHDSSRSDPDSQRSSALDDPFSILDLLLGRWFRSR

Target ORF information:

RefSeq Version	XM_006501446.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus HCLS1 associated X-1 (Hax1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501446.2

ORF Insert Sequence:

ATGACTCGAG ATGATGACGA CGATGATGAT GATGATGACG AAGCGGAGGA AGACAGAGGC 
GCGTGGGGTC GAGAGAGCTA TGCGTTTGAT GGTTCTCAGC CTCCAGAGGA ATTCGGTTTC 
AGCTTCAGCC CCAGGGGAGG GATGCGATTC CACGGCAACT TTGGCTTTGA TGATCTAGTA 
CGAGATTTTA ATAGCATCTT CAGCGAGATG GGGGCCTGGA CCTTGCCTTC CCACTCTCCT 
GAACTTCCAG GTCCTGAGTC AGAAACACCT GGTGAGAGAC TGCGGGAGGG GCAGACACTA 
CGAGACTCAA TGCTTAAGTA CCCAGATAGT CACCAACCCA GGATCTTTGA GGGGGTCTTG 
GAGAGTCATG CAAAACCTGA ATCCCCAAAA CCAGCTCCAG ATTGGGGGTC GCAGGGACCT 
TTTCATAGGT TGGATGATAC ATGGCCTGTG AGTCCCCATT CTAGAGCCAA AGAGGACAAA 
GATCTTGACT CCCAGGTTTC CCAGGAAGGT CTGGGTCCAC TTCTTCAACC CCAGCCCAAA 
TCGTATTTCA AAAGTATCTC TGTGACCAAG ATCACTAAGC CAGATGGGAC AGTGGAGGAA 
CGCCGCACTG TGGTAGACAG TGAGGGCCGG AGGGAGACCA CAGTGACCCA TCAAGAAGCC 
CATGACAGTT CCAGAAGTGA TCCAGACTCT CAGAGATCTT CAGCTTTGGA TGATCCCTTT 
TCCATTCTGG ATTTGCTACT AGGACGTTGG TTTCGGTCCC GATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hax1 ( XM_017319570.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175059.1 Mus musculus HCLS1 associated X-1 (Hax1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu31889
Clone ID Related Accession (Same CDS sequence)	XM_006501446.2 , XM_017319570.1 , XM_006501446.3 , NM_001310681.1 , XM_017319570.2
Accession Version	XM_017319570.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 765bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	HCLS1-associated protein X-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTCGAG ATGATGACGA CGATGATGAT GATGATGACG AAGCGGAGGA AGACAGAGGC 
GCGTGGGGTC GAGAGAGCTA TGCGTTTGAT GGTTCTCAGC CTCCAGAGGA ATTCGGTTTC 
AGCTTCAGCC CCAGGGGAGG GATGCGATTC CACGGCAACT TTGGCTTTGA TGATCTAGTA 
CGAGATTTTA ATAGCATCTT CAGCGAGATG GGGGCCTGGA CCTTGCCTTC CCACTCTCCT 
GAACTTCCAG GTCCTGAGTC AGAAACACCT GGTGAGAGAC TGCGGGAGGG GCAGACACTA 
CGAGACTCAA TGCTTAAGTA CCCAGATAGT CACCAACCCA GGATCTTTGA GGGGGTCTTG 
GAGAGTCATG CAAAACCTGA ATCCCCAAAA CCAGCTCCAG ATTGGGGGTC GCAGGGACCT 
TTTCATAGGT TGGATGATAC ATGGCCTGTG AGTCCCCATT CTAGAGCCAA AGAGGACAAA 
GATCTTGACT CCCAGGTTTC CCAGGAAGGT CTGGGTCCAC TTCTTCAACC CCAGCCCAAA 
TCGTATTTCA AAAGTATCTC TGTGACCAAG ATCACTAAGC CAGATGGGAC AGTGGAGGAA 
CGCCGCACTG TGGTAGACAG TGAGGGCCGG AGGGAGACCA CAGTGACCCA TCAAGAAGCC 
CATGACAGTT CCAGAAGTGA TCCAGACTCT CAGAGATCTT CAGCTTTGGA TGATCCCTTT 
TCCATTCTGG ATTTGCTACT AGGACGTTGG TTTCGGTCCC GATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175059.1
CDS	301..1065
Translation	MTRDDDDDDDDDDEAEEDRGAWGRESYAFDGSQPPEEFGFSFSPRGGMRFHGNFGFDDLVRDFNSIFSEMGAWTLPSHSPELPGPESETPGERLREGQTLRDSMLKYPDSHQPRIFEGVLESHAKPESPKPAPDWGSQGPFHRLDDTWPVSPHSRAKEDKDLDSQVSQEGLGPLLQPQPKSYFKSISVTKITKPDGTVEERRTVVDSEGRRETTVTHQEAHDSSRSDPDSQRSSALDDPFSILDLLLGRWFRSR

Target ORF information:

RefSeq Version	XM_017319570.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus HCLS1 associated X-1 (Hax1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319570.1

ORF Insert Sequence:

ATGACTCGAG ATGATGACGA CGATGATGAT GATGATGACG AAGCGGAGGA AGACAGAGGC 
GCGTGGGGTC GAGAGAGCTA TGCGTTTGAT GGTTCTCAGC CTCCAGAGGA ATTCGGTTTC 
AGCTTCAGCC CCAGGGGAGG GATGCGATTC CACGGCAACT TTGGCTTTGA TGATCTAGTA 
CGAGATTTTA ATAGCATCTT CAGCGAGATG GGGGCCTGGA CCTTGCCTTC CCACTCTCCT 
GAACTTCCAG GTCCTGAGTC AGAAACACCT GGTGAGAGAC TGCGGGAGGG GCAGACACTA 
CGAGACTCAA TGCTTAAGTA CCCAGATAGT CACCAACCCA GGATCTTTGA GGGGGTCTTG 
GAGAGTCATG CAAAACCTGA ATCCCCAAAA CCAGCTCCAG ATTGGGGGTC GCAGGGACCT 
TTTCATAGGT TGGATGATAC ATGGCCTGTG AGTCCCCATT CTAGAGCCAA AGAGGACAAA 
GATCTTGACT CCCAGGTTTC CCAGGAAGGT CTGGGTCCAC TTCTTCAACC CCAGCCCAAA 
TCGTATTTCA AAAGTATCTC TGTGACCAAG ATCACTAAGC CAGATGGGAC AGTGGAGGAA 
CGCCGCACTG TGGTAGACAG TGAGGGCCGG AGGGAGACCA CAGTGACCCA TCAAGAAGCC 
CATGACAGTT CCAGAAGTGA TCCAGACTCT CAGAGATCTT CAGCTTTGGA TGATCCCTTT 
TCCATTCTGG ATTTGCTACT AGGACGTTGG TTTCGGTCCC GATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hax1 ( NM_001282032.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001268961.1 Mus musculus HCLS1 associated X-1 (Hax1), transcript variant 2, mRNA.

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CloneID	OMu08393
Clone ID Related Accession (Same CDS sequence)	NM_001282032.1
Accession Version	NM_001282032.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 591bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-29
Organism	Mus musculus(house mouse)
Product	HCLS1-associated protein X-1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC163616.3, BY253818.1, AF465243.1, AK135138.1 and BM900159.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site and exon in the coding region, but maintains the reading frame compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK135138.1, AF465243.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCGTCT TTGATCTTTT CCGAGGCTTT TTCGGCTTTC CTGGACCTCG GAGCCACAGA 
GATCCTTTTT TTGGAGGGAT GACTCGAGAT GATGACGACG ATGATGATGA TGATGACGAA 
GCGGAGGAAG ACAGAGGCGC GTGGGGTCGA GAGAGCTATG CGTTTGATGG TTCTCAGCCT 
CCAGAGGAAT TCGGTTTCAG CTTCAGCCCC AGGGGAGGGA TGCGATTCCA CGGCAACTTT 
GGCTTTGATG ATCTATTGGA TGATACATGG CCTGTGAGTC CCCATTCTAG AGCCAAAGAG 
GACAAAGATC TTGACTCCCA GGTTTCCCAG GAAGGTCTGG GTCCACTTCT TCAACCCCAG 
CCCAAATCGT ATTTCAAAAG TATCTCTGTG ACCAAGATCA CTAAGCCAGA TGGGACAGTG 
GAGGAACGCC GCACTGTGGT AGACAGTGAG GGCCGGAGGG AGACCACAGT GACCCATCAA 
GAAGCCCATG ACAGTTCCAG AAGTGATCCA GACTCTCAGA GATCTTCAGC TTTGGATGAT 
CCCTTTTCCA TTCTGGATTT GCTACTAGGA CGTTGGTTTC GGTCCCGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001268961.1
CDS	137..727
Translation	MSVFDLFRGFFGFPGPRSHRDPFFGGMTRDDDDDDDDDDEAEEDRGAWGRESYAFDGSQPPEEFGFSFSPRGGMRFHGNFGFDDLLDDTWPVSPHSRAKEDKDLDSQVSQEGLGPLLQPQPKSYFKSISVTKITKPDGTVEERRTVVDSEGRRETTVTHQEAHDSSRSDPDSQRSSALDDPFSILDLLLGRWFRSR

Target ORF information:

RefSeq Version	NM_001282032.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus HCLS1 associated X-1 (Hax1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001282032.1

ORF Insert Sequence:

ATGAGCGTCT TTGATCTTTT CCGAGGCTTT TTCGGCTTTC CTGGACCTCG GAGCCACAGA 
GATCCTTTTT TTGGAGGGAT GACTCGAGAT GATGACGACG ATGATGATGA TGATGACGAA 
GCGGAGGAAG ACAGAGGCGC GTGGGGTCGA GAGAGCTATG CGTTTGATGG TTCTCAGCCT 
CCAGAGGAAT TCGGTTTCAG CTTCAGCCCC AGGGGAGGGA TGCGATTCCA CGGCAACTTT 
GGCTTTGATG ATCTATTGGA TGATACATGG CCTGTGAGTC CCCATTCTAG AGCCAAAGAG 
GACAAAGATC TTGACTCCCA GGTTTCCCAG GAAGGTCTGG GTCCACTTCT TCAACCCCAG 
CCCAAATCGT ATTTCAAAAG TATCTCTGTG ACCAAGATCA CTAAGCCAGA TGGGACAGTG 
GAGGAACGCC GCACTGTGGT AGACAGTGAG GGCCGGAGGG AGACCACAGT GACCCATCAA 
GAAGCCCATG ACAGTTCCAG AAGTGATCCA GACTCTCAGA GATCTTCAGC TTTGGATGAT 
CCCTTTTCCA TTCTGGATTT GCTACTAGGA CGTTGGTTTC GGTCCCGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hax1 ( XM_006501446.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501509.1 Mus musculus HCLS1 associated X-1 (Hax1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu31889
Clone ID Related Accession (Same CDS sequence)	XM_006501446.2 , XM_017319570.1 , XM_006501446.3 , NM_001310681.1 , XM_017319570.2
Accession Version	XM_006501446.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 765bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	HCLS1-associated protein X-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006501446.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTCGAG ATGATGACGA CGATGATGAT GATGATGACG AAGCGGAGGA AGACAGAGGC 
GCGTGGGGTC GAGAGAGCTA TGCGTTTGAT GGTTCTCAGC CTCCAGAGGA ATTCGGTTTC 
AGCTTCAGCC CCAGGGGAGG GATGCGATTC CACGGCAACT TTGGCTTTGA TGATCTAGTA 
CGAGATTTTA ATAGCATCTT CAGCGAGATG GGGGCCTGGA CCTTGCCTTC CCACTCTCCT 
GAACTTCCAG GTCCTGAGTC AGAAACACCT GGTGAGAGAC TGCGGGAGGG GCAGACACTA 
CGAGACTCAA TGCTTAAGTA CCCAGATAGT CACCAACCCA GGATCTTTGA GGGGGTCTTG 
GAGAGTCATG CAAAACCTGA ATCCCCAAAA CCAGCTCCAG ATTGGGGGTC GCAGGGACCT 
TTTCATAGGT TGGATGATAC ATGGCCTGTG AGTCCCCATT CTAGAGCCAA AGAGGACAAA 
GATCTTGACT CCCAGGTTTC CCAGGAAGGT CTGGGTCCAC TTCTTCAACC CCAGCCCAAA 
TCGTATTTCA AAAGTATCTC TGTGACCAAG ATCACTAAGC CAGATGGGAC AGTGGAGGAA 
CGCCGCACTG TGGTAGACAG TGAGGGCCGG AGGGAGACCA CAGTGACCCA TCAAGAAGCC 
CATGACAGTT CCAGAAGTGA TCCAGACTCT CAGAGATCTT CAGCTTTGGA TGATCCCTTT 
TCCATTCTGG ATTTGCTACT AGGACGTTGG TTTCGGTCCC GATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501509.1
CDS	296..1060
Translation	MTRDDDDDDDDDDEAEEDRGAWGRESYAFDGSQPPEEFGFSFSPRGGMRFHGNFGFDDLVRDFNSIFSEMGAWTLPSHSPELPGPESETPGERLREGQTLRDSMLKYPDSHQPRIFEGVLESHAKPESPKPAPDWGSQGPFHRLDDTWPVSPHSRAKEDKDLDSQVSQEGLGPLLQPQPKSYFKSISVTKITKPDGTVEERRTVVDSEGRRETTVTHQEAHDSSRSDPDSQRSSALDDPFSILDLLLGRWFRSR

Target ORF information:

RefSeq Version	XM_006501446.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus HCLS1 associated X-1 (Hax1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501446.3

ORF Insert Sequence:

ATGACTCGAG ATGATGACGA CGATGATGAT GATGATGACG AAGCGGAGGA AGACAGAGGC 
GCGTGGGGTC GAGAGAGCTA TGCGTTTGAT GGTTCTCAGC CTCCAGAGGA ATTCGGTTTC 
AGCTTCAGCC CCAGGGGAGG GATGCGATTC CACGGCAACT TTGGCTTTGA TGATCTAGTA 
CGAGATTTTA ATAGCATCTT CAGCGAGATG GGGGCCTGGA CCTTGCCTTC CCACTCTCCT 
GAACTTCCAG GTCCTGAGTC AGAAACACCT GGTGAGAGAC TGCGGGAGGG GCAGACACTA 
CGAGACTCAA TGCTTAAGTA CCCAGATAGT CACCAACCCA GGATCTTTGA GGGGGTCTTG 
GAGAGTCATG CAAAACCTGA ATCCCCAAAA CCAGCTCCAG ATTGGGGGTC GCAGGGACCT 
TTTCATAGGT TGGATGATAC ATGGCCTGTG AGTCCCCATT CTAGAGCCAA AGAGGACAAA 
GATCTTGACT CCCAGGTTTC CCAGGAAGGT CTGGGTCCAC TTCTTCAACC CCAGCCCAAA 
TCGTATTTCA AAAGTATCTC TGTGACCAAG ATCACTAAGC CAGATGGGAC AGTGGAGGAA 
CGCCGCACTG TGGTAGACAG TGAGGGCCGG AGGGAGACCA CAGTGACCCA TCAAGAAGCC 
CATGACAGTT CCAGAAGTGA TCCAGACTCT CAGAGATCTT CAGCTTTGGA TGATCCCTTT 
TCCATTCTGG ATTTGCTACT AGGACGTTGG TTTCGGTCCC GATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hax1 ( XM_017319570.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175059.1 Mus musculus HCLS1 associated X-1 (Hax1), transcript variant X2, mRNA.

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CloneID	OMu31889
Clone ID Related Accession (Same CDS sequence)	XM_006501446.2 , XM_017319570.1 , XM_006501446.3 , NM_001310681.1 , XM_017319570.2
Accession Version	XM_017319570.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 765bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	HCLS1-associated protein X-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017319570.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTCGAG ATGATGACGA CGATGATGAT GATGATGACG AAGCGGAGGA AGACAGAGGC 
GCGTGGGGTC GAGAGAGCTA TGCGTTTGAT GGTTCTCAGC CTCCAGAGGA ATTCGGTTTC 
AGCTTCAGCC CCAGGGGAGG GATGCGATTC CACGGCAACT TTGGCTTTGA TGATCTAGTA 
CGAGATTTTA ATAGCATCTT CAGCGAGATG GGGGCCTGGA CCTTGCCTTC CCACTCTCCT 
GAACTTCCAG GTCCTGAGTC AGAAACACCT GGTGAGAGAC TGCGGGAGGG GCAGACACTA 
CGAGACTCAA TGCTTAAGTA CCCAGATAGT CACCAACCCA GGATCTTTGA GGGGGTCTTG 
GAGAGTCATG CAAAACCTGA ATCCCCAAAA CCAGCTCCAG ATTGGGGGTC GCAGGGACCT 
TTTCATAGGT TGGATGATAC ATGGCCTGTG AGTCCCCATT CTAGAGCCAA AGAGGACAAA 
GATCTTGACT CCCAGGTTTC CCAGGAAGGT CTGGGTCCAC TTCTTCAACC CCAGCCCAAA 
TCGTATTTCA AAAGTATCTC TGTGACCAAG ATCACTAAGC CAGATGGGAC AGTGGAGGAA 
CGCCGCACTG TGGTAGACAG TGAGGGCCGG AGGGAGACCA CAGTGACCCA TCAAGAAGCC 
CATGACAGTT CCAGAAGTGA TCCAGACTCT CAGAGATCTT CAGCTTTGGA TGATCCCTTT 
TCCATTCTGG ATTTGCTACT AGGACGTTGG TTTCGGTCCC GATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175059.1
CDS	300..1064
Translation	MTRDDDDDDDDDDEAEEDRGAWGRESYAFDGSQPPEEFGFSFSPRGGMRFHGNFGFDDLVRDFNSIFSEMGAWTLPSHSPELPGPESETPGERLREGQTLRDSMLKYPDSHQPRIFEGVLESHAKPESPKPAPDWGSQGPFHRLDDTWPVSPHSRAKEDKDLDSQVSQEGLGPLLQPQPKSYFKSISVTKITKPDGTVEERRTVVDSEGRRETTVTHQEAHDSSRSDPDSQRSSALDDPFSILDLLLGRWFRSR

Target ORF information:

RefSeq Version	XM_017319570.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus HCLS1 associated X-1 (Hax1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319570.2

ORF Insert Sequence:

ATGACTCGAG ATGATGACGA CGATGATGAT GATGATGACG AAGCGGAGGA AGACAGAGGC 
GCGTGGGGTC GAGAGAGCTA TGCGTTTGAT GGTTCTCAGC CTCCAGAGGA ATTCGGTTTC 
AGCTTCAGCC CCAGGGGAGG GATGCGATTC CACGGCAACT TTGGCTTTGA TGATCTAGTA 
CGAGATTTTA ATAGCATCTT CAGCGAGATG GGGGCCTGGA CCTTGCCTTC CCACTCTCCT 
GAACTTCCAG GTCCTGAGTC AGAAACACCT GGTGAGAGAC TGCGGGAGGG GCAGACACTA 
CGAGACTCAA TGCTTAAGTA CCCAGATAGT CACCAACCCA GGATCTTTGA GGGGGTCTTG 
GAGAGTCATG CAAAACCTGA ATCCCCAAAA CCAGCTCCAG ATTGGGGGTC GCAGGGACCT 
TTTCATAGGT TGGATGATAC ATGGCCTGTG AGTCCCCATT CTAGAGCCAA AGAGGACAAA 
GATCTTGACT CCCAGGTTTC CCAGGAAGGT CTGGGTCCAC TTCTTCAACC CCAGCCCAAA 
TCGTATTTCA AAAGTATCTC TGTGACCAAG ATCACTAAGC CAGATGGGAC AGTGGAGGAA 
CGCCGCACTG TGGTAGACAG TGAGGGCCGG AGGGAGACCA CAGTGACCCA TCAAGAAGCC 
CATGACAGTT CCAGAAGTGA TCCAGACTCT CAGAGATCTT CAGCTTTGGA TGATCCCTTT 
TCCATTCTGG ATTTGCTACT AGGACGTTGG TTTCGGTCCC GATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hax1 ( XM_017319569.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175058.1 Mus musculus HCLS1 associated X-1 (Hax1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu80614
Clone ID Related Accession (Same CDS sequence)	XM_017319569.1
Accession Version	XM_017319569.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 834bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	HCLS1-associated protein X-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGTCT TTGATCTTTT CCGAGGCTTT TTCGGCTTTC CTGGACCTCG GAGCCACAGA 
GATCCTTTTT TTGGAGGGAT GACTCGAGAT GATGACGACG ATGATGATGA TGATGACGAA 
GCGGAGGAAG ACAGAGGCGC GTGGGGTCGA GAGAGCTATG CGTTTGATGG TTCTCAGCCT 
CCAGAGGAAT TCGGTTTCAG CTTCAGCCCC AGGGGAGGGA TGCGATTCCA CGGCAACTTT 
GGCTTTGATG ATCTAGTACG AGATTTTAAT AGCATCTTCA GCGAGATGGG GGCCTGGACC 
TTGCCTTCCC ACTCTCCTGG TCCTGAGTCA GAAACACCTG GTGAGAGACT GCGGGAGGGG 
CAGACACTAC GAGACTCAAT GCTTAAGTAC CCAGATAGTC ACCAACCCAG GATCTTTGAG 
GGGGTCTTGG AGAGTCATGC AAAACCTGAA TCCCCAAAAC CAGCTCCAGA TTGGGGGTCG 
CAGGGACCTT TTCATAGGTT GGATGATACA TGGCCTGTGA GTCCCCATTC TAGAGCCAAA 
GAGGACAAAG ATCTTGACTC CCAGGTTTCC CAGGAAGGTC TGGGTCCACT TCTTCAACCC 
CAGCCCAAAT CGTATTTCAA AAGTATCTCT GTGACCAAGA TCACTAAGCC AGATGGGACA 
GTGGAGGAAC GCCGCACTGT GGTAGACAGT GAGGGCCGGA GGGAGACCAC AGTGACCCAT 
CAAGAAGCCC ATGACAGTTC CAGAAGTGAT CCAGACTCTC AGAGATCTTC AGCTTTGGAT 
GATCCCTTTT CCATTCTGGA TTTGCTACTA GGACGTTGGT TTCGGTCCCG ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175058.1
CDS	110..943
Translation	MSVFDLFRGFFGFPGPRSHRDPFFGGMTRDDDDDDDDDDEAEEDRGAWGRESYAFDGSQPPEEFGFSFSPRGGMRFHGNFGFDDLVRDFNSIFSEMGAWTLPSHSPGPESETPGERLREGQTLRDSMLKYPDSHQPRIFEGVLESHAKPESPKPAPDWGSQGPFHRLDDTWPVSPHSRAKEDKDLDSQVSQEGLGPLLQPQPKSYFKSISVTKITKPDGTVEERRTVVDSEGRRETTVTHQEAHDSSRSDPDSQRSSALDDPFSILDLLLGRWFRSR

Target ORF information:

RefSeq Version	XM_017319569.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus HCLS1 associated X-1 (Hax1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319569.1

ORF Insert Sequence:

ATGAGCGTCT TTGATCTTTT CCGAGGCTTT TTCGGCTTTC CTGGACCTCG GAGCCACAGA 
GATCCTTTTT TTGGAGGGAT GACTCGAGAT GATGACGACG ATGATGATGA TGATGACGAA 
GCGGAGGAAG ACAGAGGCGC GTGGGGTCGA GAGAGCTATG CGTTTGATGG TTCTCAGCCT 
CCAGAGGAAT TCGGTTTCAG CTTCAGCCCC AGGGGAGGGA TGCGATTCCA CGGCAACTTT 
GGCTTTGATG ATCTAGTACG AGATTTTAAT AGCATCTTCA GCGAGATGGG GGCCTGGACC 
TTGCCTTCCC ACTCTCCTGG TCCTGAGTCA GAAACACCTG GTGAGAGACT GCGGGAGGGG 
CAGACACTAC GAGACTCAAT GCTTAAGTAC CCAGATAGTC ACCAACCCAG GATCTTTGAG 
GGGGTCTTGG AGAGTCATGC AAAACCTGAA TCCCCAAAAC CAGCTCCAGA TTGGGGGTCG 
CAGGGACCTT TTCATAGGTT GGATGATACA TGGCCTGTGA GTCCCCATTC TAGAGCCAAA 
GAGGACAAAG ATCTTGACTC CCAGGTTTCC CAGGAAGGTC TGGGTCCACT TCTTCAACCC 
CAGCCCAAAT CGTATTTCAA AAGTATCTCT GTGACCAAGA TCACTAAGCC AGATGGGACA 
GTGGAGGAAC GCCGCACTGT GGTAGACAGT GAGGGCCGGA GGGAGACCAC AGTGACCCAT 
CAAGAAGCCC ATGACAGTTC CAGAAGTGAT CCAGACTCTC AGAGATCTTC AGCTTTGGAT 
GATCCCTTTT CCATTCTGGA TTTGCTACTA GGACGTTGGT TTCGGTCCCG ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The antiapoptotic protein HAX-1 mediates half of phospholamban's inhibitory activity on calcium cycling and contractility in the heart.
The Journal of biological chemistry293(1)359-367(2018 Jan)
Bidwell PA,Haghighi K,Kranias EG

HAX-1 regulates SERCA2a oxidation and degradation.
Journal of molecular and cellular cardiology114220-233(2017 Nov)
Bidwell PA,Liu GS,Nagarajan N,Lam CK,Haghighi K,Gardner G,Cai WF,Zhao W,Mugge L,Vafiadaki E,Sanoudou D,Rubinstein J,Lebeche D,Hajjar R,Sadoshima J,Kranias EG

Mitochondrial Omi/HtrA2 Promotes Caspase Activation Through Cleavage of HAX-1 in Aging Heart.
Rejuvenation research20(3)183-192(2017 Jun)
Liu X,Lei J,Wang K,Ma L,Liu D,Du Y,Wu Y,Zhang S,Wang W,Ma X,Liu H

MLF1 is a proapoptotic antagonist of HOP complex-mediated survival.
Biochimica et biophysica acta1864(4)719-727(2017 04)
Sun Y,Chao JR,Xu W,Pourpak A,Boyd K,Moshiach S,Qi GY,Fu A,Shao HR,Pounds S,Morris SW

Kv3.3 Channels Bind Hax-1 and Arp2/3 to Assemble a Stable Local Actin Network that Regulates Channel Gating.
Cell165(2)434-448(2016 Apr)
Zhang Y,Zhang XF,Fleming MR,Amiri A,El-Hassar L,Surguchev AA,Hyland C,Jenkins DP,Desai R,Brown MR,Gazula VR,Waters MF,Large CH,Horvath TL,Navaratnam D,Vaccarino FM,Forscher P,Kaczmarek LK

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Hax1 cDNA ORF clone, Mus musculus(house mouse)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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