Pappa2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pappa2
Entrez Gene ID	23850
Full Name	pappalysin 2
Synonyms	PAPP-A2,PLAC3,Pappe
General protein information	Preferred Names pappalysin 2 Names pappalysin-2 placenta-specific 3 pregnancy-associated plasma preproprotein-A2 pregnancy-associated plasma protein-E
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1\|1 H1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001085376.3	NP_001078845.1	pappalysin-2 precursor
XM_006496824.4	XP_006496887.1	pappalysin-2 isoform X1
XM_011238806.2	XP_011237108.1	pappalysin-2 isoform X1
XM_006496823.3	XP_006496886.1	pappalysin-2 isoform X1
XM_006496824.3	XP_006496887.1	pappalysin-2 isoform X1
NM_001085376.2	NP_001078845.1	pappalysin-2 precursor

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	pappa2	XP_695417.4
Homo sapiens (human)	PAPPA2	NP_064714.2
Mus musculus (house mouse)	Pappa2	NP_001078845.1
	PAPPA2	XP_002808308.1
Rattus norvegicus (Norway rat)	LOC680415	XP_002728071.2
Pan troglodytes (chimpanzee)	PAPPA2	XP_514020.2
Canis lupus familiaris (dog)	PAPPA2	XP_537179.2
Bos taurus (cattle)	PAPPA2	XP_002694146.1
Gallus gallus (chicken)	PAPPA2	XP_426627.4
Xenopus tropicalis (tropical clawed frog)	pappa2	XP_002931515.2

Gene Ontology
Bibliography

Related articles in PubMed

PAPP-A2 a new key regulator of growth.
Banaszak-Ziemska M, Niedziela M
Endokrynologia Polska68(6)682-691(2017)

PAPP-A2 expression by osteoblasts is required for normal postnatal growth in mice.
Amiri N, Christians JK
Growth hormone & IGF research : official journal of the Growth Hormone Research Society and the International IGF Research Society25(6)274-80(2015 Dec)

Pappa2 deletion in mice affects male but not female fertility.
Christians JK, King AY, Rogowska MD, Hessels SM
Reproductive biology and endocrinology : RB&E13109(2015 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Locally-produced Pappa2 in osteoblasts, epiphysis, and metaphysis, is required for normal postnatal bone growth.
Title: PAPP-A2 expression by osteoblasts is required for normal postnatal growth in mice.

Despite a dramatic disruption of the balance between circulating IGF-I, IGFBP-3 and -5, we found no effects of Pappa2 deletion on glucose metabolism, weight gain or adiposity on a high-fat diet.
Title: Pappa2 deletion alters IGFBPs but has little effect on glucose disposal or adiposity.

Pappa2 deletion had no effect on female reproduction and subtle effects on male fertility.
Title: Pappa2 deletion in mice affects male but not female fertility.

Pappa2 is responsible for the effects of the previously-identified QTL, demonstrating that natural variation in the Pappa2 gene contributes to variation in postnatal growth in mice
Title: Pregnancy associated plasma protein A2 (PAPP-A2) affects bone size and shape and contributes to natural variation in postnatal growth in mice.

investigation of biological role of PAPP-A2 using knockout mice, heterozygotes, primary fibroblasts: The most striking phenotype of PAPP-A2 KO mice was postnatal growth retardation. PAPP-A2 KO mice were fertile, but exhibited compromised fecundity.
Title: Pregnancy-associated plasma protein-A2 (PAPP-A2): tissue expression and biological consequences of gene knockout in mice.

The following Pappa2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pappa2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu02923	XM_006496824.4 Latest version!	Mus musculus pappalysin 2 (Pappa2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu02923	NM_001085376.3 Latest version!	Mus musculus pappalysin 2 (Pappa2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu02923	XM_011238806.2 Latest version!	Mus musculus pappalysin 2 (Pappa2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu02923	XM_006496823.3 Latest version!	Mus musculus pappalysin 2 (Pappa2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
View Old Accession Versions >>
OMu02923	XM_006496824.3	Mus musculus pappalysin 2 (Pappa2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu02923	NM_001085376.2	Mus musculus pappalysin 2 (Pappa2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pappa2 ( XM_006496824.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496887.1 Mus musculus pappalysin 2 (Pappa2), transcript variant X1, mRNA.

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CloneID	OMu02923
Clone ID Related Accession (Same CDS sequence)	NM_001085376.3 , XM_006496824.4 , XM_011238806.2 , XM_006496823.3 , XM_006496824.3 , NM_001085376.2
Accession Version	XM_006496824.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5370bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	pappalysin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006496824.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGTT GGAAGGTCCT GAGAATAAGC CTGGTGGTCC TGACTGGGTG GGCACTCAGT 
ACCACCAACT CAGAGCAGGG CTGGACACGC AAGAAATTCC TGGCCCAGAA GGAACACCTG 
AACCAAGTGC TGTTGGAAGG GGAACGCTGT TGGCTGGGGT ATAAGGTTCG AAGACCCAGG 
ACTGCTCCAC AGCACCATCT CTTTGGGGTC TACCCAAGCC GCCTCGAAAA CTACCCAAGG 
TCCTATCCTG TGGGGACACA GCAGCAAACC CTGTATGCAG AATGGAGTCA GCGAAGCCCA 
GAGAGACAGG CTCCGAGTGG TATTCCCCGA GACCTGACTG AAAACCCAGC AGGAGCAAGG 
AGAGAGTCTC AGCAGCCAGC TGCCCCGTGG GTAGGGGATG GTCCTATTGG GCAATCGCAG 
CTGCTGAGAA ATGATGACAC TTATCTTGGC GAGGGAGGAT CTAAGGAGGC TCTAGGTGAG 
CCTGCGATTC AGGGAAGCTC AGAAATTGCT GTCTCCACCA TTGCTACCTT CGCATATCAG 
AAGGGACCCA CAGCAGAGAC CCAGAGGAAG GGCCAGTCCA AGTCTAGGCT TCCTCGCCAA 
GTGCAGAAGA GGCAGGCAGA AGACATGACT GGAGACCCCC AGAATACCCC TCAAGGTTTC 
CAGCTTTGGC CTAAAGAGCC ACTTAAGCAT GGAGATAGTG ACAGTCCATT GGAGGGCACC 
ATCCAAAATG GTGGAGGGGC CTCTCTCCGG AGAGTAGAGA CCTTTAACTC CCAAGGAGGA 
TTGCCTGTAC TGTATTTCAC TGGGAAGAGG GAGCGGCTGT TGCTGCGTCC AGAAGTGCTG 
GCTGAGATTC CCCGGGAGGC GTTCACAGTG GAAGCCTGGG TGAGACCAGA GGGAGGACAG 
AGCAACCCAG CCATCATTGC AGGTGTGTTT GATAACTGCT CCCACACAGT CAATGACAAG 
GGCTGGGCCC TGGGGATCCG CTCAGGGAAG GACAAGGGGT GGAGAGATGC TCGCTTCTTC 
TTTTCCCTCC GCACTGACCG GATGAAGAAA GCCACTATTG TGACTGGCCA CAGCCGTTAC 
CAGCCAGGCA TGTGGACTCA TGTAGCAGCC ACTTACGATG GACAGCATAC AGCTCTGTAT 
GTGGATGGCA CTCGAGTGGC TAGTAGTCGT GACCAGTTTG GACCGCTAAA TAGCCCTTTC 
ATGGCGTCTT GCCGCTCTTT AATCCTGGGA GGGGACAGCT CCGAGGATGG ACATTATTTC 
CGTGGTTACC TGGGCACACT GGTAATCTGG TCGACCGCCC TTTCACAAAC CCACCTCCAG 
CACAGTCCCC TGCATCCAAG TGCAGCAGAT GAGTTGACCA CCCTGATCCT GACAGCCACT 
TTCGATCCTC TGATGGAACA GTGGGCTCCA TTTAGAGATG ACTTATATCC CCGGCTGGAG 
GTTCTCCAGG ACTCTGAGTC ACAGCCTGAG ATTCTGTCAC CTTTACAACC CCCACCTTGT 
GGGCAAACAG CTTGTGACAA TGTGGAACTC ATCTCCCAGT ACAATAAGCA TGGGCCCCTT 
CGTAGAGAGA AAGTGATCCG CTACCAGGTG GTAAACATCT ATGATGATGA GGGTCTGCAT 
CCCATTGTGA GTGATCATCA GATCCGCCGT CAGCATGAGG CCCTCAACCA GGCCTTCAGC 
CGCTACAATA TCAGCTGGCA GCTGAGTGTC CACCGAGTCC ACAACTCCAC CCTGCGCCAC 
CGGGTTGTGC TTGTTAATTG TGAGCCCAGC AAGATTGGCA ATGACCACTG TGACCCTGAA 
TGTGAGCATC CACTCACCGG CTTCGATGGG GGCGATTGCC GCCTGCAGGG ACGCTGCTAC 
TCCTGGAATC GCAGGGATGG CCTCTGTCAC GTGGAGTGCA ACAACATGCT GAATGACTTC 
GATGATGGAG ACTGCTGCGA TCCGGAAGTG ACTGATGTCC GAAAGACTTG CTTTGATCCT 
GACTCACCCA AGAGGGCATA CATGAGTGTG AAGGAACTAA AGGAGGCCTT GCAGCTCAAC 
AGCACTCACT TCCTTAATGT CTACTTCGCC AGCTCAGTCC GGGAAGACCT TGCAGGTTCT 
GCCACATGGC CCTGGGACAA GGAAGCTCTC AGTCACCTGG GTGGTGTTGT CCTCAACCCA 
ACCTATTATG GCATGCTTGG CCACACCAAC ATCATGATCC ATGAGGTGGG ACATGTCCTG 
GGACTCTACC ATGTATTCAA AGGAGTCAGT GAAAGAGAAT CCTGTGATGA TCCCTGTAGA 
GAGACAGTGC CATCCATGGA GACAGGAGAC CTGTGTGCTG ACACTGCTCC CACACCCAAG 
AGCAAGCTGT GTCGGGACCC AGAGCCAGCT AATGACACCT GTGGCTTCAC CCTCTTCCCA 
GGGGCTCCGT TCAATAACTA CATGAGCTAC ACAGATGATG AGTGCACTGA CAACTTCACC 
CCTAACCAAG TGGCCCGGAT GCATTGCTAC TTGGACCTCG TATACCAGCA ATGGAGTGAA 
AGCCAAAAAC CTACCCCCAT ACCCATACCA CCCATGGTTA TTGGGCAAAC CCACAAGTCC 
CTTACTATCC ATTGGCTGCC TCCAATCAGT GGAGTTGTGT ATGATAGGGC CCCTGACAGC 
ATGTGTAGTG CCTGCACTGA AGATGGGACA TTCCGTCAGT ATGTATACAA GGCATCCTCT 
GGGCGGGTGT GTGACTCTTC AGGTTACTGG ACTCCAGAGG AGGCTGTGGG GCCTCCTGAT 
GTCGATCAAC CCTGTGAGCC TAGCTTGCAG GCCTGGAGTC CTGAGCTCCA CCTAAACCAC 
ATGAATATGA CCGTGCCCTG CCCAGCAGAA GGCTGTAGCT TGGAGTTGCT TTTTCAATAC 
CCGGTCCAGG CGGATACCCT CACCCTCTGG GTCACTTACC TCTCCATGAA CTCTTCCAAG 
GCATTCTTTG ACATAGAGAT CTTGCTAGAA CACAAAAAAT CTATGCACCT TGGACCTTTA 
AACACTTTTT GTGACATACC GCTCACCATC AAACTCCACA TGGATGAGAA GGTCATGGGA 
GCGAAAGTCT ACACCTTTGA TGAACGAATG GAAATTGATG CTGCTCTCCT GACCTCTCGG 
CCCAATAGTT CCTTGTGTTC TGGATGCAAG CCTATGAGTT ACCAGGTTCT TCGAGAGCCT 
CCTTTTTCCA GTGGCTTGCC CATGGTGGTG ACACATCCAC ACAGAAAGTT CACAGATACG 
GAGGTCATCC CTGGGCAGAT GTATCAGTAC CAAGTTCAAG CTGAAGCTGT AGGGGAACTC 
GGAGAAGCTT CACCTCCTCT GAGCCACATT CACGGAGGCC CTTACTGTGG AGATGGGAAG 
GTAGAAAGGA GTATCGGAGA GATGTGTGAT GATGGAGACC TGTTGAATGG AGATGGGTGC 
TCAAGAGCAT GTGAACTGGA GGAAGGCTTC AATTGTGCAG GGGAGCCAAG CCTTTGCTAC 
AGGTATGAGG GAGATGGGGT TTGTGAAGAT TTTGAGAAGG AAAGCATCAT CACAGACTGT 
GGCCTCCACA CTCCTGAAGG ATATTTGGAT CAGTGGGCTA GCCAGGCTTA TTCCTACCAT 
GAAGACAAGG AGAAATGTCC TGTTTCCCTG GTAACAGGAG AACCTCATTC AATGATCTGT 
ACTTCATGGC ACCCGGATTC ATCCCCATAT CATTCCTTTC CTGGCTGGTT TCCCTGTGTC 
TTCAGTCTGA AGAAAGACCA GGATGCAGGC AGTGAGCAGG CTAAAGATAG CCTGCAGAAG 
GACAATGAAA TTTGGCTTGA AGTGTGTTTC AATAGGCCAG GGGTTGCAGT GGCAATTTAC 
ATTTTCTTGG CATCGGATGG CGTGACCCCT GGGGAGCATC AGCAGGCAAC AGTGACCCTT 
TACCTGATTG ATGTCAGTGG AAGCAACCAC TCTCTTGGAA CCTATGGACT GTCGTGTCAA 
CAAAATCCAC TGGTTATCAA TGTGAGCCAT CATGTGAATG TCTACCCCCA ACATACCTCC 
TCCATGCTGC TGAATTTTTC ATCCCCACTG GTGGGCATCT CAGCAGTGGC TCTAAGGACA 
TCCTCCCAGA CCAGTTCTTC AGCTCCCAGT AACTGCATCC CAGAGCAAAA TTATGAGGGA 
AAGAGCTGTG CTCAACGACC TTGTGGAGAA CAGGGAAGAT GTGCGCCATT GCTACTCGAT 
CATACGGACA TGGTGAATTG TACCTCCAGT AGCCCAGGTC ACATGGAGTG CGCTATCACT 
TGTCAAAGGG GGTATGTCCT TCAGACCAGC AGTGGGCAGT ACCTGAGGAA CATGCAGAAA 
GAGATTCTCC TTACTTGTTC CTCTGGCCAC TGGGACAAGG ATGTGATCTG CAAGCGTCTT 
GACTGTGGTG TCCCAGACTC CTCCTTGGTG AATTATGCAA ACTTCTCCTG CTTGGAGGGT 
ACAGACTTTC TGAAACAATG TTCCATCTCG TGTGTCCCAC CGGCTAAGCT CCAAGGACTG 
AACCCATGGC TGACATGTCT TGAAGATGGA CTCTGGTCTC TCCCTGAAGT CTACTGCAAG 
TTGGAATGTG AAGCTCCTCC AGTTATTCCC AATGCCAATC TGCTCCTGCC ACATTTCCTG 
GAAGGCAACC ATGATGTGGG CACCATCTGC AAATATGAGT GCAAACCAGG CTACTATGTG 
AAGGAGACTT CAGGAAGTCA AGGCAAGAAC AAGTTCCTGA AGATCCAGTG CCTGGATGAT 
GGAAGCTGGG AACAAGGGAG CTGCGTCCCC GTGGTTTGTG AGCCTCCTCC TCCGGTGTTT 
GAAGGCATGT ACGAATGTAC TGATGGCTTC AAGCTGGACA GCCAGTGTGT GCTCAACTGT 
AACCAGGAAA CGGAAGGGAT TCCCATCCTC TGCACTAAAG AGGGGTTGTG GACCCAGGAA 
TTCAAGTTGT GTGAAAACCT GCAAGGAGAA TGCCCACCAC CCACCTCAGA GCTGAATTCT 
GTGGAGTACA AGTGTGGACA GGGATATGGG ATCGGTGCAG TGTGCTCCCC ATCATGTGTA 
ATCCCCCCTA GTGACCCTGT GATACTACCA GAGAATGTGA CTACTGACAC TTTGGAGCAC 
TGGATGGAAC CTGTCAAAGT CCAGAGTATC GTGTGTACCG GCAGGCGCCA GTGGCACCCA 
GATCCCTCTC TGGTGCACTG CATCCAGTCA TGTGAGCCTT TCCAAGCAGA TGGTTGGTGT 
GACACTATTA ATAACCGGGC CTACTGCAAC TATGACGGGG GAGACTGCTG CTCTTCCACA 
CTCTCTTCCA AGAAGGTCAT TCCATTTGCT GCTGATTGTG ACTTGGATGA ATGCACCTGT 
CGAGACCCCA AGGCAGAAGA AAACCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496887.1
CDS	908..6277
Translation	MMCWKVLRISLVVLTGWALSTTNSEQGWTRKKFLAQKEHLNQVLLEGERCWLGYKVRRPRTAPQHHLFGVYPSRLENYPRSYPVGTQQQTLYAEWSQRSPERQAPSGIPRDLTENPAGARRESQQPAAPWVGDGPIGQSQLLRNDDTYLGEGGSKEALGEPAIQGSSEIAVSTIATFAYQKGPTAETQRKGQSKSRLPRQVQKRQAEDMTGDPQNTPQGFQLWPKEPLKHGDSDSPLEGTIQNGGGASLRRVETFNSQGGLPVLYFTGKRERLLLRPEVLAEIPREAFTVEAWVRPEGGQSNPAIIAGVFDNCSHTVNDKGWALGIRSGKDKGWRDARFFFSLRTDRMKKATIVTGHSRYQPGMWTHVAATYDGQHTALYVDGTRVASSRDQFGPLNSPFMASCRSLILGGDSSEDGHYFRGYLGTLVIWSTALSQTHLQHSPLHPSAADELTTLILTATFDPLMEQWAPFRDDLYPRLEVLQDSESQPEILSPLQPPPCGQTACDNVELISQYNKHGPLRREKVIRYQVVNIYDDEGLHPIVSDHQIRRQHEALNQAFSRYNISWQLSVHRVHNSTLRHRVVLVNCEPSKIGNDHCDPECEHPLTGFDGGDCRLQGRCYSWNRRDGLCHVECNNMLNDFDDGDCCDPEVTDVRKTCFDPDSPKRAYMSVKELKEALQLNSTHFLNVYFASSVREDLAGSATWPWDKEALSHLGGVVLNPTYYGMLGHTNIMIHEVGHVLGLYHVFKGVSERESCDDPCRETVPSMETGDLCADTAPTPKSKLCRDPEPANDTCGFTLFPGAPFNNYMSYTDDECTDNFTPNQVARMHCYLDLVYQQWSESQKPTPIPIPPMVIGQTHKSLTIHWLPPISGVVYDRAPDSMCSACTEDGTFRQYVYKASSGRVCDSSGYWTPEEAVGPPDVDQPCEPSLQAWSPELHLNHMNMTVPCPAEGCSLELLFQYPVQADTLTLWVTYLSMNSSKAFFDIEILLEHKKSMHLGPLNTFCDIPLTIKLHMDEKVMGAKVYTFDERMEIDAALLTSRPNSSLCSGCKPMSYQVLREPPFSSGLPMVVTHPHRKFTDTEVIPGQMYQYQVQAEAVGELGEASPPLSHIHGGPYCGDGKVERSIGEMCDDGDLLNGDGCSRACELEEGFNCAGEPSLCYRYEGDGVCEDFEKESIITDCGLHTPEGYLDQWASQAYSYHEDKEKCPVSLVTGEPHSMICTSWHPDSSPYHSFPGWFPCVFSLKKDQDAGSEQAKDSLQKDNEIWLEVCFNRPGVAVAIYIFLASDGVTPGEHQQATVTLYLIDVSGSNHSLGTYGLSCQQNPLVINVSHHVNVYPQHTSSMLLNFSSPLVGISAVALRTSSQTSSSAPSNCIPEQNYEGKSCAQRPCGEQGRCAPLLLDHTDMVNCTSSSPGHMECAITCQRGYVLQTSSGQYLRNMQKEILLTCSSGHWDKDVICKRLDCGVPDSSLVNYANFSCLEGTDFLKQCSISCVPPAKLQGLNPWLTCLEDGLWSLPEVYCKLECEAPPVIPNANLLLPHFLEGNHDVGTICKYECKPGYYVKETSGSQGKNKFLKIQCLDDGSWEQGSCVPVVCEPPPPVFEGMYECTDGFKLDSQCVLNCNQETEGIPILCTKEGLWTQEFKLCENLQGECPPPTSELNSVEYKCGQGYGIGAVCSPSCVIPPSDPVILPENVTTDTLEHWMEPVKVQSIVCTGRRQWHPDPSLVHCIQSCEPFQADGWCDTINNRAYCNYDGGDCCSSTLSSKKVIPFAADCDLDECTCRDPKAEENQ

Target ORF information:

RefSeq Version	XM_006496824.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus pappalysin 2 (Pappa2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496824.4

ORF Insert Sequence:

ATGATGTGTT GGAAGGTCCT GAGAATAAGC CTGGTGGTCC TGACTGGGTG GGCACTCAGT 
ACCACCAACT CAGAGCAGGG CTGGACACGC AAGAAATTCC TGGCCCAGAA GGAACACCTG 
AACCAAGTGC TGTTGGAAGG GGAACGCTGT TGGCTGGGGT ATAAGGTTCG AAGACCCAGG 
ACTGCTCCAC AGCACCATCT CTTTGGGGTC TACCCAAGCC GCCTCGAAAA CTACCCAAGG 
TCCTATCCTG TGGGGACACA GCAGCAAACC CTGTATGCAG AATGGAGTCA GCGAAGCCCA 
GAGAGACAGG CTCCGAGTGG TATTCCCCGA GACCTGACTG AAAACCCAGC AGGAGCAAGG 
AGAGAGTCTC AGCAGCCAGC TGCCCCGTGG GTAGGGGATG GTCCTATTGG GCAATCGCAG 
CTGCTGAGAA ATGATGACAC TTATCTTGGC GAGGGAGGAT CTAAGGAGGC TCTAGGTGAG 
CCTGCGATTC AGGGAAGCTC AGAAATTGCT GTCTCCACCA TTGCTACCTT CGCATATCAG 
AAGGGACCCA CAGCAGAGAC CCAGAGGAAG GGCCAGTCCA AGTCTAGGCT TCCTCGCCAA 
GTGCAGAAGA GGCAGGCAGA AGACATGACT GGAGACCCCC AGAATACCCC TCAAGGTTTC 
CAGCTTTGGC CTAAAGAGCC ACTTAAGCAT GGAGATAGTG ACAGTCCATT GGAGGGCACC 
ATCCAAAATG GTGGAGGGGC CTCTCTCCGG AGAGTAGAGA CCTTTAACTC CCAAGGAGGA 
TTGCCTGTAC TGTATTTCAC TGGGAAGAGG GAGCGGCTGT TGCTGCGTCC AGAAGTGCTG 
GCTGAGATTC CCCGGGAGGC GTTCACAGTG GAAGCCTGGG TGAGACCAGA GGGAGGACAG 
AGCAACCCAG CCATCATTGC AGGTGTGTTT GATAACTGCT CCCACACAGT CAATGACAAG 
GGCTGGGCCC TGGGGATCCG CTCAGGGAAG GACAAGGGGT GGAGAGATGC TCGCTTCTTC 
TTTTCCCTCC GCACTGACCG GATGAAGAAA GCCACTATTG TGACTGGCCA CAGCCGTTAC 
CAGCCAGGCA TGTGGACTCA TGTAGCAGCC ACTTACGATG GACAGCATAC AGCTCTGTAT 
GTGGATGGCA CTCGAGTGGC TAGTAGTCGT GACCAGTTTG GACCGCTAAA TAGCCCTTTC 
ATGGCGTCTT GCCGCTCTTT AATCCTGGGA GGGGACAGCT CCGAGGATGG ACATTATTTC 
CGTGGTTACC TGGGCACACT GGTAATCTGG TCGACCGCCC TTTCACAAAC CCACCTCCAG 
CACAGTCCCC TGCATCCAAG TGCAGCAGAT GAGTTGACCA CCCTGATCCT GACAGCCACT 
TTCGATCCTC TGATGGAACA GTGGGCTCCA TTTAGAGATG ACTTATATCC CCGGCTGGAG 
GTTCTCCAGG ACTCTGAGTC ACAGCCTGAG ATTCTGTCAC CTTTACAACC CCCACCTTGT 
GGGCAAACAG CTTGTGACAA TGTGGAACTC ATCTCCCAGT ACAATAAGCA TGGGCCCCTT 
CGTAGAGAGA AAGTGATCCG CTACCAGGTG GTAAACATCT ATGATGATGA GGGTCTGCAT 
CCCATTGTGA GTGATCATCA GATCCGCCGT CAGCATGAGG CCCTCAACCA GGCCTTCAGC 
CGCTACAATA TCAGCTGGCA GCTGAGTGTC CACCGAGTCC ACAACTCCAC CCTGCGCCAC 
CGGGTTGTGC TTGTTAATTG TGAGCCCAGC AAGATTGGCA ATGACCACTG TGACCCTGAA 
TGTGAGCATC CACTCACCGG CTTCGATGGG GGCGATTGCC GCCTGCAGGG ACGCTGCTAC 
TCCTGGAATC GCAGGGATGG CCTCTGTCAC GTGGAGTGCA ACAACATGCT GAATGACTTC 
GATGATGGAG ACTGCTGCGA TCCGGAAGTG ACTGATGTCC GAAAGACTTG CTTTGATCCT 
GACTCACCCA AGAGGGCATA CATGAGTGTG AAGGAACTAA AGGAGGCCTT GCAGCTCAAC 
AGCACTCACT TCCTTAATGT CTACTTCGCC AGCTCAGTCC GGGAAGACCT TGCAGGTTCT 
GCCACATGGC CCTGGGACAA GGAAGCTCTC AGTCACCTGG GTGGTGTTGT CCTCAACCCA 
ACCTATTATG GCATGCTTGG CCACACCAAC ATCATGATCC ATGAGGTGGG ACATGTCCTG 
GGACTCTACC ATGTATTCAA AGGAGTCAGT GAAAGAGAAT CCTGTGATGA TCCCTGTAGA 
GAGACAGTGC CATCCATGGA GACAGGAGAC CTGTGTGCTG ACACTGCTCC CACACCCAAG 
AGCAAGCTGT GTCGGGACCC AGAGCCAGCT AATGACACCT GTGGCTTCAC CCTCTTCCCA 
GGGGCTCCGT TCAATAACTA CATGAGCTAC ACAGATGATG AGTGCACTGA CAACTTCACC 
CCTAACCAAG TGGCCCGGAT GCATTGCTAC TTGGACCTCG TATACCAGCA ATGGAGTGAA 
AGCCAAAAAC CTACCCCCAT ACCCATACCA CCCATGGTTA TTGGGCAAAC CCACAAGTCC 
CTTACTATCC ATTGGCTGCC TCCAATCAGT GGAGTTGTGT ATGATAGGGC CCCTGACAGC 
ATGTGTAGTG CCTGCACTGA AGATGGGACA TTCCGTCAGT ATGTATACAA GGCATCCTCT 
GGGCGGGTGT GTGACTCTTC AGGTTACTGG ACTCCAGAGG AGGCTGTGGG GCCTCCTGAT 
GTCGATCAAC CCTGTGAGCC TAGCTTGCAG GCCTGGAGTC CTGAGCTCCA CCTAAACCAC 
ATGAATATGA CCGTGCCCTG CCCAGCAGAA GGCTGTAGCT TGGAGTTGCT TTTTCAATAC 
CCGGTCCAGG CGGATACCCT CACCCTCTGG GTCACTTACC TCTCCATGAA CTCTTCCAAG 
GCATTCTTTG ACATAGAGAT CTTGCTAGAA CACAAAAAAT CTATGCACCT TGGACCTTTA 
AACACTTTTT GTGACATACC GCTCACCATC AAACTCCACA TGGATGAGAA GGTCATGGGA 
GCGAAAGTCT ACACCTTTGA TGAACGAATG GAAATTGATG CTGCTCTCCT GACCTCTCGG 
CCCAATAGTT CCTTGTGTTC TGGATGCAAG CCTATGAGTT ACCAGGTTCT TCGAGAGCCT 
CCTTTTTCCA GTGGCTTGCC CATGGTGGTG ACACATCCAC ACAGAAAGTT CACAGATACG 
GAGGTCATCC CTGGGCAGAT GTATCAGTAC CAAGTTCAAG CTGAAGCTGT AGGGGAACTC 
GGAGAAGCTT CACCTCCTCT GAGCCACATT CACGGAGGCC CTTACTGTGG AGATGGGAAG 
GTAGAAAGGA GTATCGGAGA GATGTGTGAT GATGGAGACC TGTTGAATGG AGATGGGTGC 
TCAAGAGCAT GTGAACTGGA GGAAGGCTTC AATTGTGCAG GGGAGCCAAG CCTTTGCTAC 
AGGTATGAGG GAGATGGGGT TTGTGAAGAT TTTGAGAAGG AAAGCATCAT CACAGACTGT 
GGCCTCCACA CTCCTGAAGG ATATTTGGAT CAGTGGGCTA GCCAGGCTTA TTCCTACCAT 
GAAGACAAGG AGAAATGTCC TGTTTCCCTG GTAACAGGAG AACCTCATTC AATGATCTGT 
ACTTCATGGC ACCCGGATTC ATCCCCATAT CATTCCTTTC CTGGCTGGTT TCCCTGTGTC 
TTCAGTCTGA AGAAAGACCA GGATGCAGGC AGTGAGCAGG CTAAAGATAG CCTGCAGAAG 
GACAATGAAA TTTGGCTTGA AGTGTGTTTC AATAGGCCAG GGGTTGCAGT GGCAATTTAC 
ATTTTCTTGG CATCGGATGG CGTGACCCCT GGGGAGCATC AGCAGGCAAC AGTGACCCTT 
TACCTGATTG ATGTCAGTGG AAGCAACCAC TCTCTTGGAA CCTATGGACT GTCGTGTCAA 
CAAAATCCAC TGGTTATCAA TGTGAGCCAT CATGTGAATG TCTACCCCCA ACATACCTCC 
TCCATGCTGC TGAATTTTTC ATCCCCACTG GTGGGCATCT CAGCAGTGGC TCTAAGGACA 
TCCTCCCAGA CCAGTTCTTC AGCTCCCAGT AACTGCATCC CAGAGCAAAA TTATGAGGGA 
AAGAGCTGTG CTCAACGACC TTGTGGAGAA CAGGGAAGAT GTGCGCCATT GCTACTCGAT 
CATACGGACA TGGTGAATTG TACCTCCAGT AGCCCAGGTC ACATGGAGTG CGCTATCACT 
TGTCAAAGGG GGTATGTCCT TCAGACCAGC AGTGGGCAGT ACCTGAGGAA CATGCAGAAA 
GAGATTCTCC TTACTTGTTC CTCTGGCCAC TGGGACAAGG ATGTGATCTG CAAGCGTCTT 
GACTGTGGTG TCCCAGACTC CTCCTTGGTG AATTATGCAA ACTTCTCCTG CTTGGAGGGT 
ACAGACTTTC TGAAACAATG TTCCATCTCG TGTGTCCCAC CGGCTAAGCT CCAAGGACTG 
AACCCATGGC TGACATGTCT TGAAGATGGA CTCTGGTCTC TCCCTGAAGT CTACTGCAAG 
TTGGAATGTG AAGCTCCTCC AGTTATTCCC AATGCCAATC TGCTCCTGCC ACATTTCCTG 
GAAGGCAACC ATGATGTGGG CACCATCTGC AAATATGAGT GCAAACCAGG CTACTATGTG 
AAGGAGACTT CAGGAAGTCA AGGCAAGAAC AAGTTCCTGA AGATCCAGTG CCTGGATGAT 
GGAAGCTGGG AACAAGGGAG CTGCGTCCCC GTGGTTTGTG AGCCTCCTCC TCCGGTGTTT 
GAAGGCATGT ACGAATGTAC TGATGGCTTC AAGCTGGACA GCCAGTGTGT GCTCAACTGT 
AACCAGGAAA CGGAAGGGAT TCCCATCCTC TGCACTAAAG AGGGGTTGTG GACCCAGGAA 
TTCAAGTTGT GTGAAAACCT GCAAGGAGAA TGCCCACCAC CCACCTCAGA GCTGAATTCT 
GTGGAGTACA AGTGTGGACA GGGATATGGG ATCGGTGCAG TGTGCTCCCC ATCATGTGTA 
ATCCCCCCTA GTGACCCTGT GATACTACCA GAGAATGTGA CTACTGACAC TTTGGAGCAC 
TGGATGGAAC CTGTCAAAGT CCAGAGTATC GTGTGTACCG GCAGGCGCCA GTGGCACCCA 
GATCCCTCTC TGGTGCACTG CATCCAGTCA TGTGAGCCTT TCCAAGCAGA TGGTTGGTGT 
GACACTATTA ATAACCGGGC CTACTGCAAC TATGACGGGG GAGACTGCTG CTCTTCCACA 
CTCTCTTCCA AGAAGGTCAT TCCATTTGCT GCTGATTGTG ACTTGGATGA ATGCACCTGT 
CGAGACCCCA AGGCAGAAGA AAACCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pappa2 ( NM_001085376.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001078845.1 Mus musculus pappalysin 2 (Pappa2), mRNA.

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CloneID	OMu02923
Clone ID Related Accession (Same CDS sequence)	NM_001085376.3 , XM_006496824.4 , XM_011238806.2 , XM_006496823.3 , XM_006496824.3 , NM_001085376.2
Accession Version	NM_001085376.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5370bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Mus musculus(house mouse)
Product	pappalysin-2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC162173.4, AC115116.6 and AC158910.3. On Jun 20, 2019 this sequence version replaced NM_001085376.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01164134 [ECO:0000348] ##Evidence-Data-END##

ATGATGTGTT GGAAGGTCCT GAGAATAAGC CTGGTGGTCC TGACTGGGTG GGCACTCAGT 
ACCACCAACT CAGAGCAGGG CTGGACACGC AAGAAATTCC TGGCCCAGAA GGAACACCTG 
AACCAAGTGC TGTTGGAAGG GGAACGCTGT TGGCTGGGGT ATAAGGTTCG AAGACCCAGG 
ACTGCTCCAC AGCACCATCT CTTTGGGGTC TACCCAAGCC GCCTCGAAAA CTACCCAAGG 
TCCTATCCTG TGGGGACACA GCAGCAAACC CTGTATGCAG AATGGAGTCA GCGAAGCCCA 
GAGAGACAGG CTCCGAGTGG TATTCCCCGA GACCTGACTG AAAACCCAGC AGGAGCAAGG 
AGAGAGTCTC AGCAGCCAGC TGCCCCGTGG GTAGGGGATG GTCCTATTGG GCAATCGCAG 
CTGCTGAGAA ATGATGACAC TTATCTTGGC GAGGGAGGAT CTAAGGAGGC TCTAGGTGAG 
CCTGCGATTC AGGGAAGCTC AGAAATTGCT GTCTCCACCA TTGCTACCTT CGCATATCAG 
AAGGGACCCA CAGCAGAGAC CCAGAGGAAG GGCCAGTCCA AGTCTAGGCT TCCTCGCCAA 
GTGCAGAAGA GGCAGGCAGA AGACATGACT GGAGACCCCC AGAATACCCC TCAAGGTTTC 
CAGCTTTGGC CTAAAGAGCC ACTTAAGCAT GGAGATAGTG ACAGTCCATT GGAGGGCACC 
ATCCAAAATG GTGGAGGGGC CTCTCTCCGG AGAGTAGAGA CCTTTAACTC CCAAGGAGGA 
TTGCCTGTAC TGTATTTCAC TGGGAAGAGG GAGCGGCTGT TGCTGCGTCC AGAAGTGCTG 
GCTGAGATTC CCCGGGAGGC GTTCACAGTG GAAGCCTGGG TGAGACCAGA GGGAGGACAG 
AGCAACCCAG CCATCATTGC AGGTGTGTTT GATAACTGCT CCCACACAGT CAATGACAAG 
GGCTGGGCCC TGGGGATCCG CTCAGGGAAG GACAAGGGGT GGAGAGATGC TCGCTTCTTC 
TTTTCCCTCC GCACTGACCG GATGAAGAAA GCCACTATTG TGACTGGCCA CAGCCGTTAC 
CAGCCAGGCA TGTGGACTCA TGTAGCAGCC ACTTACGATG GACAGCATAC AGCTCTGTAT 
GTGGATGGCA CTCGAGTGGC TAGTAGTCGT GACCAGTTTG GACCGCTAAA TAGCCCTTTC 
ATGGCGTCTT GCCGCTCTTT AATCCTGGGA GGGGACAGCT CCGAGGATGG ACATTATTTC 
CGTGGTTACC TGGGCACACT GGTAATCTGG TCGACCGCCC TTTCACAAAC CCACCTCCAG 
CACAGTCCCC TGCATCCAAG TGCAGCAGAT GAGTTGACCA CCCTGATCCT GACAGCCACT 
TTCGATCCTC TGATGGAACA GTGGGCTCCA TTTAGAGATG ACTTATATCC CCGGCTGGAG 
GTTCTCCAGG ACTCTGAGTC ACAGCCTGAG ATTCTGTCAC CTTTACAACC CCCACCTTGT 
GGGCAAACAG CTTGTGACAA TGTGGAACTC ATCTCCCAGT ACAATAAGCA TGGGCCCCTT 
CGTAGAGAGA AAGTGATCCG CTACCAGGTG GTAAACATCT ATGATGATGA GGGTCTGCAT 
CCCATTGTGA GTGATCATCA GATCCGCCGT CAGCATGAGG CCCTCAACCA GGCCTTCAGC 
CGCTACAATA TCAGCTGGCA GCTGAGTGTC CACCGAGTCC ACAACTCCAC CCTGCGCCAC 
CGGGTTGTGC TTGTTAATTG TGAGCCCAGC AAGATTGGCA ATGACCACTG TGACCCTGAA 
TGTGAGCATC CACTCACCGG CTTCGATGGG GGCGATTGCC GCCTGCAGGG ACGCTGCTAC 
TCCTGGAATC GCAGGGATGG CCTCTGTCAC GTGGAGTGCA ACAACATGCT GAATGACTTC 
GATGATGGAG ACTGCTGCGA TCCGGAAGTG ACTGATGTCC GAAAGACTTG CTTTGATCCT 
GACTCACCCA AGAGGGCATA CATGAGTGTG AAGGAACTAA AGGAGGCCTT GCAGCTCAAC 
AGCACTCACT TCCTTAATGT CTACTTCGCC AGCTCAGTCC GGGAAGACCT TGCAGGTTCT 
GCCACATGGC CCTGGGACAA GGAAGCTCTC AGTCACCTGG GTGGTGTTGT CCTCAACCCA 
ACCTATTATG GCATGCTTGG CCACACCAAC ATCATGATCC ATGAGGTGGG ACATGTCCTG 
GGACTCTACC ATGTATTCAA AGGAGTCAGT GAAAGAGAAT CCTGTGATGA TCCCTGTAGA 
GAGACAGTGC CATCCATGGA GACAGGAGAC CTGTGTGCTG ACACTGCTCC CACACCCAAG 
AGCAAGCTGT GTCGGGACCC AGAGCCAGCT AATGACACCT GTGGCTTCAC CCTCTTCCCA 
GGGGCTCCGT TCAATAACTA CATGAGCTAC ACAGATGATG AGTGCACTGA CAACTTCACC 
CCTAACCAAG TGGCCCGGAT GCATTGCTAC TTGGACCTCG TATACCAGCA ATGGAGTGAA 
AGCCAAAAAC CTACCCCCAT ACCCATACCA CCCATGGTTA TTGGGCAAAC CCACAAGTCC 
CTTACTATCC ATTGGCTGCC TCCAATCAGT GGAGTTGTGT ATGATAGGGC CCCTGACAGC 
ATGTGTAGTG CCTGCACTGA AGATGGGACA TTCCGTCAGT ATGTATACAA GGCATCCTCT 
GGGCGGGTGT GTGACTCTTC AGGTTACTGG ACTCCAGAGG AGGCTGTGGG GCCTCCTGAT 
GTCGATCAAC CCTGTGAGCC TAGCTTGCAG GCCTGGAGTC CTGAGCTCCA CCTAAACCAC 
ATGAATATGA CCGTGCCCTG CCCAGCAGAA GGCTGTAGCT TGGAGTTGCT TTTTCAATAC 
CCGGTCCAGG CGGATACCCT CACCCTCTGG GTCACTTACC TCTCCATGAA CTCTTCCAAG 
GCATTCTTTG ACATAGAGAT CTTGCTAGAA CACAAAAAAT CTATGCACCT TGGACCTTTA 
AACACTTTTT GTGACATACC GCTCACCATC AAACTCCACA TGGATGAGAA GGTCATGGGA 
GCGAAAGTCT ACACCTTTGA TGAACGAATG GAAATTGATG CTGCTCTCCT GACCTCTCGG 
CCCAATAGTT CCTTGTGTTC TGGATGCAAG CCTATGAGTT ACCAGGTTCT TCGAGAGCCT 
CCTTTTTCCA GTGGCTTGCC CATGGTGGTG ACACATCCAC ACAGAAAGTT CACAGATACG 
GAGGTCATCC CTGGGCAGAT GTATCAGTAC CAAGTTCAAG CTGAAGCTGT AGGGGAACTC 
GGAGAAGCTT CACCTCCTCT GAGCCACATT CACGGAGGCC CTTACTGTGG AGATGGGAAG 
GTAGAAAGGA GTATCGGAGA GATGTGTGAT GATGGAGACC TGTTGAATGG AGATGGGTGC 
TCAAGAGCAT GTGAACTGGA GGAAGGCTTC AATTGTGCAG GGGAGCCAAG CCTTTGCTAC 
AGGTATGAGG GAGATGGGGT TTGTGAAGAT TTTGAGAAGG AAAGCATCAT CACAGACTGT 
GGCCTCCACA CTCCTGAAGG ATATTTGGAT CAGTGGGCTA GCCAGGCTTA TTCCTACCAT 
GAAGACAAGG AGAAATGTCC TGTTTCCCTG GTAACAGGAG AACCTCATTC AATGATCTGT 
ACTTCATGGC ACCCGGATTC ATCCCCATAT CATTCCTTTC CTGGCTGGTT TCCCTGTGTC 
TTCAGTCTGA AGAAAGACCA GGATGCAGGC AGTGAGCAGG CTAAAGATAG CCTGCAGAAG 
GACAATGAAA TTTGGCTTGA AGTGTGTTTC AATAGGCCAG GGGTTGCAGT GGCAATTTAC 
ATTTTCTTGG CATCGGATGG CGTGACCCCT GGGGAGCATC AGCAGGCAAC AGTGACCCTT 
TACCTGATTG ATGTCAGTGG AAGCAACCAC TCTCTTGGAA CCTATGGACT GTCGTGTCAA 
CAAAATCCAC TGGTTATCAA TGTGAGCCAT CATGTGAATG TCTACCCCCA ACATACCTCC 
TCCATGCTGC TGAATTTTTC ATCCCCACTG GTGGGCATCT CAGCAGTGGC TCTAAGGACA 
TCCTCCCAGA CCAGTTCTTC AGCTCCCAGT AACTGCATCC CAGAGCAAAA TTATGAGGGA 
AAGAGCTGTG CTCAACGACC TTGTGGAGAA CAGGGAAGAT GTGCGCCATT GCTACTCGAT 
CATACGGACA TGGTGAATTG TACCTCCAGT AGCCCAGGTC ACATGGAGTG CGCTATCACT 
TGTCAAAGGG GGTATGTCCT TCAGACCAGC AGTGGGCAGT ACCTGAGGAA CATGCAGAAA 
GAGATTCTCC TTACTTGTTC CTCTGGCCAC TGGGACAAGG ATGTGATCTG CAAGCGTCTT 
GACTGTGGTG TCCCAGACTC CTCCTTGGTG AATTATGCAA ACTTCTCCTG CTTGGAGGGT 
ACAGACTTTC TGAAACAATG TTCCATCTCG TGTGTCCCAC CGGCTAAGCT CCAAGGACTG 
AACCCATGGC TGACATGTCT TGAAGATGGA CTCTGGTCTC TCCCTGAAGT CTACTGCAAG 
TTGGAATGTG AAGCTCCTCC AGTTATTCCC AATGCCAATC TGCTCCTGCC ACATTTCCTG 
GAAGGCAACC ATGATGTGGG CACCATCTGC AAATATGAGT GCAAACCAGG CTACTATGTG 
AAGGAGACTT CAGGAAGTCA AGGCAAGAAC AAGTTCCTGA AGATCCAGTG CCTGGATGAT 
GGAAGCTGGG AACAAGGGAG CTGCGTCCCC GTGGTTTGTG AGCCTCCTCC TCCGGTGTTT 
GAAGGCATGT ACGAATGTAC TGATGGCTTC AAGCTGGACA GCCAGTGTGT GCTCAACTGT 
AACCAGGAAA CGGAAGGGAT TCCCATCCTC TGCACTAAAG AGGGGTTGTG GACCCAGGAA 
TTCAAGTTGT GTGAAAACCT GCAAGGAGAA TGCCCACCAC CCACCTCAGA GCTGAATTCT 
GTGGAGTACA AGTGTGGACA GGGATATGGG ATCGGTGCAG TGTGCTCCCC ATCATGTGTA 
ATCCCCCCTA GTGACCCTGT GATACTACCA GAGAATGTGA CTACTGACAC TTTGGAGCAC 
TGGATGGAAC CTGTCAAAGT CCAGAGTATC GTGTGTACCG GCAGGCGCCA GTGGCACCCA 
GATCCCTCTC TGGTGCACTG CATCCAGTCA TGTGAGCCTT TCCAAGCAGA TGGTTGGTGT 
GACACTATTA ATAACCGGGC CTACTGCAAC TATGACGGGG GAGACTGCTG CTCTTCCACA 
CTCTCTTCCA AGAAGGTCAT TCCATTTGCT GCTGATTGTG ACTTGGATGA ATGCACCTGT 
CGAGACCCCA AGGCAGAAGA AAACCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001078845.1
CDS	393..5762
Translation	MMCWKVLRISLVVLTGWALSTTNSEQGWTRKKFLAQKEHLNQVLLEGERCWLGYKVRRPRTAPQHHLFGVYPSRLENYPRSYPVGTQQQTLYAEWSQRSPERQAPSGIPRDLTENPAGARRESQQPAAPWVGDGPIGQSQLLRNDDTYLGEGGSKEALGEPAIQGSSEIAVSTIATFAYQKGPTAETQRKGQSKSRLPRQVQKRQAEDMTGDPQNTPQGFQLWPKEPLKHGDSDSPLEGTIQNGGGASLRRVETFNSQGGLPVLYFTGKRERLLLRPEVLAEIPREAFTVEAWVRPEGGQSNPAIIAGVFDNCSHTVNDKGWALGIRSGKDKGWRDARFFFSLRTDRMKKATIVTGHSRYQPGMWTHVAATYDGQHTALYVDGTRVASSRDQFGPLNSPFMASCRSLILGGDSSEDGHYFRGYLGTLVIWSTALSQTHLQHSPLHPSAADELTTLILTATFDPLMEQWAPFRDDLYPRLEVLQDSESQPEILSPLQPPPCGQTACDNVELISQYNKHGPLRREKVIRYQVVNIYDDEGLHPIVSDHQIRRQHEALNQAFSRYNISWQLSVHRVHNSTLRHRVVLVNCEPSKIGNDHCDPECEHPLTGFDGGDCRLQGRCYSWNRRDGLCHVECNNMLNDFDDGDCCDPEVTDVRKTCFDPDSPKRAYMSVKELKEALQLNSTHFLNVYFASSVREDLAGSATWPWDKEALSHLGGVVLNPTYYGMLGHTNIMIHEVGHVLGLYHVFKGVSERESCDDPCRETVPSMETGDLCADTAPTPKSKLCRDPEPANDTCGFTLFPGAPFNNYMSYTDDECTDNFTPNQVARMHCYLDLVYQQWSESQKPTPIPIPPMVIGQTHKSLTIHWLPPISGVVYDRAPDSMCSACTEDGTFRQYVYKASSGRVCDSSGYWTPEEAVGPPDVDQPCEPSLQAWSPELHLNHMNMTVPCPAEGCSLELLFQYPVQADTLTLWVTYLSMNSSKAFFDIEILLEHKKSMHLGPLNTFCDIPLTIKLHMDEKVMGAKVYTFDERMEIDAALLTSRPNSSLCSGCKPMSYQVLREPPFSSGLPMVVTHPHRKFTDTEVIPGQMYQYQVQAEAVGELGEASPPLSHIHGGPYCGDGKVERSIGEMCDDGDLLNGDGCSRACELEEGFNCAGEPSLCYRYEGDGVCEDFEKESIITDCGLHTPEGYLDQWASQAYSYHEDKEKCPVSLVTGEPHSMICTSWHPDSSPYHSFPGWFPCVFSLKKDQDAGSEQAKDSLQKDNEIWLEVCFNRPGVAVAIYIFLASDGVTPGEHQQATVTLYLIDVSGSNHSLGTYGLSCQQNPLVINVSHHVNVYPQHTSSMLLNFSSPLVGISAVALRTSSQTSSSAPSNCIPEQNYEGKSCAQRPCGEQGRCAPLLLDHTDMVNCTSSSPGHMECAITCQRGYVLQTSSGQYLRNMQKEILLTCSSGHWDKDVICKRLDCGVPDSSLVNYANFSCLEGTDFLKQCSISCVPPAKLQGLNPWLTCLEDGLWSLPEVYCKLECEAPPVIPNANLLLPHFLEGNHDVGTICKYECKPGYYVKETSGSQGKNKFLKIQCLDDGSWEQGSCVPVVCEPPPPVFEGMYECTDGFKLDSQCVLNCNQETEGIPILCTKEGLWTQEFKLCENLQGECPPPTSELNSVEYKCGQGYGIGAVCSPSCVIPPSDPVILPENVTTDTLEHWMEPVKVQSIVCTGRRQWHPDPSLVHCIQSCEPFQADGWCDTINNRAYCNYDGGDCCSSTLSSKKVIPFAADCDLDECTCRDPKAEENQ

Target ORF information:

RefSeq Version	NM_001085376.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus pappalysin 2 (Pappa2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001085376.3

ORF Insert Sequence:

ATGATGTGTT GGAAGGTCCT GAGAATAAGC CTGGTGGTCC TGACTGGGTG GGCACTCAGT 
ACCACCAACT CAGAGCAGGG CTGGACACGC AAGAAATTCC TGGCCCAGAA GGAACACCTG 
AACCAAGTGC TGTTGGAAGG GGAACGCTGT TGGCTGGGGT ATAAGGTTCG AAGACCCAGG 
ACTGCTCCAC AGCACCATCT CTTTGGGGTC TACCCAAGCC GCCTCGAAAA CTACCCAAGG 
TCCTATCCTG TGGGGACACA GCAGCAAACC CTGTATGCAG AATGGAGTCA GCGAAGCCCA 
GAGAGACAGG CTCCGAGTGG TATTCCCCGA GACCTGACTG AAAACCCAGC AGGAGCAAGG 
AGAGAGTCTC AGCAGCCAGC TGCCCCGTGG GTAGGGGATG GTCCTATTGG GCAATCGCAG 
CTGCTGAGAA ATGATGACAC TTATCTTGGC GAGGGAGGAT CTAAGGAGGC TCTAGGTGAG 
CCTGCGATTC AGGGAAGCTC AGAAATTGCT GTCTCCACCA TTGCTACCTT CGCATATCAG 
AAGGGACCCA CAGCAGAGAC CCAGAGGAAG GGCCAGTCCA AGTCTAGGCT TCCTCGCCAA 
GTGCAGAAGA GGCAGGCAGA AGACATGACT GGAGACCCCC AGAATACCCC TCAAGGTTTC 
CAGCTTTGGC CTAAAGAGCC ACTTAAGCAT GGAGATAGTG ACAGTCCATT GGAGGGCACC 
ATCCAAAATG GTGGAGGGGC CTCTCTCCGG AGAGTAGAGA CCTTTAACTC CCAAGGAGGA 
TTGCCTGTAC TGTATTTCAC TGGGAAGAGG GAGCGGCTGT TGCTGCGTCC AGAAGTGCTG 
GCTGAGATTC CCCGGGAGGC GTTCACAGTG GAAGCCTGGG TGAGACCAGA GGGAGGACAG 
AGCAACCCAG CCATCATTGC AGGTGTGTTT GATAACTGCT CCCACACAGT CAATGACAAG 
GGCTGGGCCC TGGGGATCCG CTCAGGGAAG GACAAGGGGT GGAGAGATGC TCGCTTCTTC 
TTTTCCCTCC GCACTGACCG GATGAAGAAA GCCACTATTG TGACTGGCCA CAGCCGTTAC 
CAGCCAGGCA TGTGGACTCA TGTAGCAGCC ACTTACGATG GACAGCATAC AGCTCTGTAT 
GTGGATGGCA CTCGAGTGGC TAGTAGTCGT GACCAGTTTG GACCGCTAAA TAGCCCTTTC 
ATGGCGTCTT GCCGCTCTTT AATCCTGGGA GGGGACAGCT CCGAGGATGG ACATTATTTC 
CGTGGTTACC TGGGCACACT GGTAATCTGG TCGACCGCCC TTTCACAAAC CCACCTCCAG 
CACAGTCCCC TGCATCCAAG TGCAGCAGAT GAGTTGACCA CCCTGATCCT GACAGCCACT 
TTCGATCCTC TGATGGAACA GTGGGCTCCA TTTAGAGATG ACTTATATCC CCGGCTGGAG 
GTTCTCCAGG ACTCTGAGTC ACAGCCTGAG ATTCTGTCAC CTTTACAACC CCCACCTTGT 
GGGCAAACAG CTTGTGACAA TGTGGAACTC ATCTCCCAGT ACAATAAGCA TGGGCCCCTT 
CGTAGAGAGA AAGTGATCCG CTACCAGGTG GTAAACATCT ATGATGATGA GGGTCTGCAT 
CCCATTGTGA GTGATCATCA GATCCGCCGT CAGCATGAGG CCCTCAACCA GGCCTTCAGC 
CGCTACAATA TCAGCTGGCA GCTGAGTGTC CACCGAGTCC ACAACTCCAC CCTGCGCCAC 
CGGGTTGTGC TTGTTAATTG TGAGCCCAGC AAGATTGGCA ATGACCACTG TGACCCTGAA 
TGTGAGCATC CACTCACCGG CTTCGATGGG GGCGATTGCC GCCTGCAGGG ACGCTGCTAC 
TCCTGGAATC GCAGGGATGG CCTCTGTCAC GTGGAGTGCA ACAACATGCT GAATGACTTC 
GATGATGGAG ACTGCTGCGA TCCGGAAGTG ACTGATGTCC GAAAGACTTG CTTTGATCCT 
GACTCACCCA AGAGGGCATA CATGAGTGTG AAGGAACTAA AGGAGGCCTT GCAGCTCAAC 
AGCACTCACT TCCTTAATGT CTACTTCGCC AGCTCAGTCC GGGAAGACCT TGCAGGTTCT 
GCCACATGGC CCTGGGACAA GGAAGCTCTC AGTCACCTGG GTGGTGTTGT CCTCAACCCA 
ACCTATTATG GCATGCTTGG CCACACCAAC ATCATGATCC ATGAGGTGGG ACATGTCCTG 
GGACTCTACC ATGTATTCAA AGGAGTCAGT GAAAGAGAAT CCTGTGATGA TCCCTGTAGA 
GAGACAGTGC CATCCATGGA GACAGGAGAC CTGTGTGCTG ACACTGCTCC CACACCCAAG 
AGCAAGCTGT GTCGGGACCC AGAGCCAGCT AATGACACCT GTGGCTTCAC CCTCTTCCCA 
GGGGCTCCGT TCAATAACTA CATGAGCTAC ACAGATGATG AGTGCACTGA CAACTTCACC 
CCTAACCAAG TGGCCCGGAT GCATTGCTAC TTGGACCTCG TATACCAGCA ATGGAGTGAA 
AGCCAAAAAC CTACCCCCAT ACCCATACCA CCCATGGTTA TTGGGCAAAC CCACAAGTCC 
CTTACTATCC ATTGGCTGCC TCCAATCAGT GGAGTTGTGT ATGATAGGGC CCCTGACAGC 
ATGTGTAGTG CCTGCACTGA AGATGGGACA TTCCGTCAGT ATGTATACAA GGCATCCTCT 
GGGCGGGTGT GTGACTCTTC AGGTTACTGG ACTCCAGAGG AGGCTGTGGG GCCTCCTGAT 
GTCGATCAAC CCTGTGAGCC TAGCTTGCAG GCCTGGAGTC CTGAGCTCCA CCTAAACCAC 
ATGAATATGA CCGTGCCCTG CCCAGCAGAA GGCTGTAGCT TGGAGTTGCT TTTTCAATAC 
CCGGTCCAGG CGGATACCCT CACCCTCTGG GTCACTTACC TCTCCATGAA CTCTTCCAAG 
GCATTCTTTG ACATAGAGAT CTTGCTAGAA CACAAAAAAT CTATGCACCT TGGACCTTTA 
AACACTTTTT GTGACATACC GCTCACCATC AAACTCCACA TGGATGAGAA GGTCATGGGA 
GCGAAAGTCT ACACCTTTGA TGAACGAATG GAAATTGATG CTGCTCTCCT GACCTCTCGG 
CCCAATAGTT CCTTGTGTTC TGGATGCAAG CCTATGAGTT ACCAGGTTCT TCGAGAGCCT 
CCTTTTTCCA GTGGCTTGCC CATGGTGGTG ACACATCCAC ACAGAAAGTT CACAGATACG 
GAGGTCATCC CTGGGCAGAT GTATCAGTAC CAAGTTCAAG CTGAAGCTGT AGGGGAACTC 
GGAGAAGCTT CACCTCCTCT GAGCCACATT CACGGAGGCC CTTACTGTGG AGATGGGAAG 
GTAGAAAGGA GTATCGGAGA GATGTGTGAT GATGGAGACC TGTTGAATGG AGATGGGTGC 
TCAAGAGCAT GTGAACTGGA GGAAGGCTTC AATTGTGCAG GGGAGCCAAG CCTTTGCTAC 
AGGTATGAGG GAGATGGGGT TTGTGAAGAT TTTGAGAAGG AAAGCATCAT CACAGACTGT 
GGCCTCCACA CTCCTGAAGG ATATTTGGAT CAGTGGGCTA GCCAGGCTTA TTCCTACCAT 
GAAGACAAGG AGAAATGTCC TGTTTCCCTG GTAACAGGAG AACCTCATTC AATGATCTGT 
ACTTCATGGC ACCCGGATTC ATCCCCATAT CATTCCTTTC CTGGCTGGTT TCCCTGTGTC 
TTCAGTCTGA AGAAAGACCA GGATGCAGGC AGTGAGCAGG CTAAAGATAG CCTGCAGAAG 
GACAATGAAA TTTGGCTTGA AGTGTGTTTC AATAGGCCAG GGGTTGCAGT GGCAATTTAC 
ATTTTCTTGG CATCGGATGG CGTGACCCCT GGGGAGCATC AGCAGGCAAC AGTGACCCTT 
TACCTGATTG ATGTCAGTGG AAGCAACCAC TCTCTTGGAA CCTATGGACT GTCGTGTCAA 
CAAAATCCAC TGGTTATCAA TGTGAGCCAT CATGTGAATG TCTACCCCCA ACATACCTCC 
TCCATGCTGC TGAATTTTTC ATCCCCACTG GTGGGCATCT CAGCAGTGGC TCTAAGGACA 
TCCTCCCAGA CCAGTTCTTC AGCTCCCAGT AACTGCATCC CAGAGCAAAA TTATGAGGGA 
AAGAGCTGTG CTCAACGACC TTGTGGAGAA CAGGGAAGAT GTGCGCCATT GCTACTCGAT 
CATACGGACA TGGTGAATTG TACCTCCAGT AGCCCAGGTC ACATGGAGTG CGCTATCACT 
TGTCAAAGGG GGTATGTCCT TCAGACCAGC AGTGGGCAGT ACCTGAGGAA CATGCAGAAA 
GAGATTCTCC TTACTTGTTC CTCTGGCCAC TGGGACAAGG ATGTGATCTG CAAGCGTCTT 
GACTGTGGTG TCCCAGACTC CTCCTTGGTG AATTATGCAA ACTTCTCCTG CTTGGAGGGT 
ACAGACTTTC TGAAACAATG TTCCATCTCG TGTGTCCCAC CGGCTAAGCT CCAAGGACTG 
AACCCATGGC TGACATGTCT TGAAGATGGA CTCTGGTCTC TCCCTGAAGT CTACTGCAAG 
TTGGAATGTG AAGCTCCTCC AGTTATTCCC AATGCCAATC TGCTCCTGCC ACATTTCCTG 
GAAGGCAACC ATGATGTGGG CACCATCTGC AAATATGAGT GCAAACCAGG CTACTATGTG 
AAGGAGACTT CAGGAAGTCA AGGCAAGAAC AAGTTCCTGA AGATCCAGTG CCTGGATGAT 
GGAAGCTGGG AACAAGGGAG CTGCGTCCCC GTGGTTTGTG AGCCTCCTCC TCCGGTGTTT 
GAAGGCATGT ACGAATGTAC TGATGGCTTC AAGCTGGACA GCCAGTGTGT GCTCAACTGT 
AACCAGGAAA CGGAAGGGAT TCCCATCCTC TGCACTAAAG AGGGGTTGTG GACCCAGGAA 
TTCAAGTTGT GTGAAAACCT GCAAGGAGAA TGCCCACCAC CCACCTCAGA GCTGAATTCT 
GTGGAGTACA AGTGTGGACA GGGATATGGG ATCGGTGCAG TGTGCTCCCC ATCATGTGTA 
ATCCCCCCTA GTGACCCTGT GATACTACCA GAGAATGTGA CTACTGACAC TTTGGAGCAC 
TGGATGGAAC CTGTCAAAGT CCAGAGTATC GTGTGTACCG GCAGGCGCCA GTGGCACCCA 
GATCCCTCTC TGGTGCACTG CATCCAGTCA TGTGAGCCTT TCCAAGCAGA TGGTTGGTGT 
GACACTATTA ATAACCGGGC CTACTGCAAC TATGACGGGG GAGACTGCTG CTCTTCCACA 
CTCTCTTCCA AGAAGGTCAT TCCATTTGCT GCTGATTGTG ACTTGGATGA ATGCACCTGT 
CGAGACCCCA AGGCAGAAGA AAACCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pappa2 ( NM_001085376.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001078845.1 Mus musculus pappalysin 2 (Pappa2), mRNA.

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CloneID	OMu02923
Clone ID Related Accession (Same CDS sequence)	NM_001085376.3 , XM_006496824.4 , XM_011238806.2 , XM_006496823.3 , XM_006496824.3 , NM_001085376.2
Accession Version	NM_001085376.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5370bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-08-10
Organism	Mus musculus(house mouse)
Product	pappalysin-2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC162173.4, AK084006.1, BC094560.1, CN703031.1 and CN700808.1. On Aug 29, 2007 this sequence version replaced NM_001085376.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01164134 [ECO:0000348] ##Evidence-Data-END##

ATGATGTGTT GGAAGGTCCT GAGAATAAGC CTGGTGGTCC TGACTGGGTG GGCACTCAGT 
ACCACCAACT CAGAGCAGGG CTGGACACGC AAGAAATTCC TGGCCCAGAA GGAACACCTG 
AACCAAGTGC TGTTGGAAGG GGAACGCTGT TGGCTGGGGT ATAAGGTTCG AAGACCCAGG 
ACTGCTCCAC AGCACCATCT CTTTGGGGTC TACCCAAGCC GCCTCGAAAA CTACCCAAGG 
TCCTATCCTG TGGGGACACA GCAGCAAACC CTGTATGCAG AATGGAGTCA GCGAAGCCCA 
GAGAGACAGG CTCCGAGTGG TATTCCCCGA GACCTGACTG AAAACCCAGC AGGAGCAAGG 
AGAGAGTCTC AGCAGCCAGC TGCCCCGTGG GTAGGGGATG GTCCTATTGG GCAATCGCAG 
CTGCTGAGAA ATGATGACAC TTATCTTGGC GAGGGAGGAT CTAAGGAGGC TCTAGGTGAG 
CCTGCGATTC AGGGAAGCTC AGAAATTGCT GTCTCCACCA TTGCTACCTT CGCATATCAG 
AAGGGACCCA CAGCAGAGAC CCAGAGGAAG GGCCAGTCCA AGTCTAGGCT TCCTCGCCAA 
GTGCAGAAGA GGCAGGCAGA AGACATGACT GGAGACCCCC AGAATACCCC TCAAGGTTTC 
CAGCTTTGGC CTAAAGAGCC ACTTAAGCAT GGAGATAGTG ACAGTCCATT GGAGGGCACC 
ATCCAAAATG GTGGAGGGGC CTCTCTCCGG AGAGTAGAGA CCTTTAACTC CCAAGGAGGA 
TTGCCTGTAC TGTATTTCAC TGGGAAGAGG GAGCGGCTGT TGCTGCGTCC AGAAGTGCTG 
GCTGAGATTC CCCGGGAGGC GTTCACAGTG GAAGCCTGGG TGAGACCAGA GGGAGGACAG 
AGCAACCCAG CCATCATTGC AGGTGTGTTT GATAACTGCT CCCACACAGT CAATGACAAG 
GGCTGGGCCC TGGGGATCCG CTCAGGGAAG GACAAGGGGT GGAGAGATGC TCGCTTCTTC 
TTTTCCCTCC GCACTGACCG GATGAAGAAA GCCACTATTG TGACTGGCCA CAGCCGTTAC 
CAGCCAGGCA TGTGGACTCA TGTAGCAGCC ACTTACGATG GACAGCATAC AGCTCTGTAT 
GTGGATGGCA CTCGAGTGGC TAGTAGTCGT GACCAGTTTG GACCGCTAAA TAGCCCTTTC 
ATGGCGTCTT GCCGCTCTTT AATCCTGGGA GGGGACAGCT CCGAGGATGG ACATTATTTC 
CGTGGTTACC TGGGCACACT GGTAATCTGG TCGACCGCCC TTTCACAAAC CCACCTCCAG 
CACAGTCCCC TGCATCCAAG TGCAGCAGAT GAGTTGACCA CCCTGATCCT GACAGCCACT 
TTCGATCCTC TGATGGAACA GTGGGCTCCA TTTAGAGATG ACTTATATCC CCGGCTGGAG 
GTTCTCCAGG ACTCTGAGTC ACAGCCTGAG ATTCTGTCAC CTTTACAACC CCCACCTTGT 
GGGCAAACAG CTTGTGACAA TGTGGAACTC ATCTCCCAGT ACAATAAGCA TGGGCCCCTT 
CGTAGAGAGA AAGTGATCCG CTACCAGGTG GTAAACATCT ATGATGATGA GGGTCTGCAT 
CCCATTGTGA GTGATCATCA GATCCGCCGT CAGCATGAGG CCCTCAACCA GGCCTTCAGC 
CGCTACAATA TCAGCTGGCA GCTGAGTGTC CACCGAGTCC ACAACTCCAC CCTGCGCCAC 
CGGGTTGTGC TTGTTAATTG TGAGCCCAGC AAGATTGGCA ATGACCACTG TGACCCTGAA 
TGTGAGCATC CACTCACCGG CTTCGATGGG GGCGATTGCC GCCTGCAGGG ACGCTGCTAC 
TCCTGGAATC GCAGGGATGG CCTCTGTCAC GTGGAGTGCA ACAACATGCT GAATGACTTC 
GATGATGGAG ACTGCTGCGA TCCGGAAGTG ACTGATGTCC GAAAGACTTG CTTTGATCCT 
GACTCACCCA AGAGGGCATA CATGAGTGTG AAGGAACTAA AGGAGGCCTT GCAGCTCAAC 
AGCACTCACT TCCTTAATGT CTACTTCGCC AGCTCAGTCC GGGAAGACCT TGCAGGTTCT 
GCCACATGGC CCTGGGACAA GGAAGCTCTC AGTCACCTGG GTGGTGTTGT CCTCAACCCA 
ACCTATTATG GCATGCTTGG CCACACCAAC ATCATGATCC ATGAGGTGGG ACATGTCCTG 
GGACTCTACC ATGTATTCAA AGGAGTCAGT GAAAGAGAAT CCTGTGATGA TCCCTGTAGA 
GAGACAGTGC CATCCATGGA GACAGGAGAC CTGTGTGCTG ACACTGCTCC CACACCCAAG 
AGCAAGCTGT GTCGGGACCC AGAGCCAGCT AATGACACCT GTGGCTTCAC CCTCTTCCCA 
GGGGCTCCGT TCAATAACTA CATGAGCTAC ACAGATGATG AGTGCACTGA CAACTTCACC 
CCTAACCAAG TGGCCCGGAT GCATTGCTAC TTGGACCTCG TATACCAGCA ATGGAGTGAA 
AGCCAAAAAC CTACCCCCAT ACCCATACCA CCCATGGTTA TTGGGCAAAC CCACAAGTCC 
CTTACTATCC ATTGGCTGCC TCCAATCAGT GGAGTTGTGT ATGATAGGGC CCCTGACAGC 
ATGTGTAGTG CCTGCACTGA AGATGGGACA TTCCGTCAGT ATGTATACAA GGCATCCTCT 
GGGCGGGTGT GTGACTCTTC AGGTTACTGG ACTCCAGAGG AGGCTGTGGG GCCTCCTGAT 
GTCGATCAAC CCTGTGAGCC TAGCTTGCAG GCCTGGAGTC CTGAGCTCCA CCTAAACCAC 
ATGAATATGA CCGTGCCCTG CCCAGCAGAA GGCTGTAGCT TGGAGTTGCT TTTTCAATAC 
CCGGTCCAGG CGGATACCCT CACCCTCTGG GTCACTTACC TCTCCATGAA CTCTTCCAAG 
GCATTCTTTG ACATAGAGAT CTTGCTAGAA CACAAAAAAT CTATGCACCT TGGACCTTTA 
AACACTTTTT GTGACATACC GCTCACCATC AAACTCCACA TGGATGAGAA GGTCATGGGA 
GCGAAAGTCT ACACCTTTGA TGAACGAATG GAAATTGATG CTGCTCTCCT GACCTCTCGG 
CCCAATAGTT CCTTGTGTTC TGGATGCAAG CCTATGAGTT ACCAGGTTCT TCGAGAGCCT 
CCTTTTTCCA GTGGCTTGCC CATGGTGGTG ACACATCCAC ACAGAAAGTT CACAGATACG 
GAGGTCATCC CTGGGCAGAT GTATCAGTAC CAAGTTCAAG CTGAAGCTGT AGGGGAACTC 
GGAGAAGCTT CACCTCCTCT GAGCCACATT CACGGAGGCC CTTACTGTGG AGATGGGAAG 
GTAGAAAGGA GTATCGGAGA GATGTGTGAT GATGGAGACC TGTTGAATGG AGATGGGTGC 
TCAAGAGCAT GTGAACTGGA GGAAGGCTTC AATTGTGCAG GGGAGCCAAG CCTTTGCTAC 
AGGTATGAGG GAGATGGGGT TTGTGAAGAT TTTGAGAAGG AAAGCATCAT CACAGACTGT 
GGCCTCCACA CTCCTGAAGG ATATTTGGAT CAGTGGGCTA GCCAGGCTTA TTCCTACCAT 
GAAGACAAGG AGAAATGTCC TGTTTCCCTG GTAACAGGAG AACCTCATTC AATGATCTGT 
ACTTCATGGC ACCCGGATTC ATCCCCATAT CATTCCTTTC CTGGCTGGTT TCCCTGTGTC 
TTCAGTCTGA AGAAAGACCA GGATGCAGGC AGTGAGCAGG CTAAAGATAG CCTGCAGAAG 
GACAATGAAA TTTGGCTTGA AGTGTGTTTC AATAGGCCAG GGGTTGCAGT GGCAATTTAC 
ATTTTCTTGG CATCGGATGG CGTGACCCCT GGGGAGCATC AGCAGGCAAC AGTGACCCTT 
TACCTGATTG ATGTCAGTGG AAGCAACCAC TCTCTTGGAA CCTATGGACT GTCGTGTCAA 
CAAAATCCAC TGGTTATCAA TGTGAGCCAT CATGTGAATG TCTACCCCCA ACATACCTCC 
TCCATGCTGC TGAATTTTTC ATCCCCACTG GTGGGCATCT CAGCAGTGGC TCTAAGGACA 
TCCTCCCAGA CCAGTTCTTC AGCTCCCAGT AACTGCATCC CAGAGCAAAA TTATGAGGGA 
AAGAGCTGTG CTCAACGACC TTGTGGAGAA CAGGGAAGAT GTGCGCCATT GCTACTCGAT 
CATACGGACA TGGTGAATTG TACCTCCAGT AGCCCAGGTC ACATGGAGTG CGCTATCACT 
TGTCAAAGGG GGTATGTCCT TCAGACCAGC AGTGGGCAGT ACCTGAGGAA CATGCAGAAA 
GAGATTCTCC TTACTTGTTC CTCTGGCCAC TGGGACAAGG ATGTGATCTG CAAGCGTCTT 
GACTGTGGTG TCCCAGACTC CTCCTTGGTG AATTATGCAA ACTTCTCCTG CTTGGAGGGT 
ACAGACTTTC TGAAACAATG TTCCATCTCG TGTGTCCCAC CGGCTAAGCT CCAAGGACTG 
AACCCATGGC TGACATGTCT TGAAGATGGA CTCTGGTCTC TCCCTGAAGT CTACTGCAAG 
TTGGAATGTG AAGCTCCTCC AGTTATTCCC AATGCCAATC TGCTCCTGCC ACATTTCCTG 
GAAGGCAACC ATGATGTGGG CACCATCTGC AAATATGAGT GCAAACCAGG CTACTATGTG 
AAGGAGACTT CAGGAAGTCA AGGCAAGAAC AAGTTCCTGA AGATCCAGTG CCTGGATGAT 
GGAAGCTGGG AACAAGGGAG CTGCGTCCCC GTGGTTTGTG AGCCTCCTCC TCCGGTGTTT 
GAAGGCATGT ACGAATGTAC TGATGGCTTC AAGCTGGACA GCCAGTGTGT GCTCAACTGT 
AACCAGGAAA CGGAAGGGAT TCCCATCCTC TGCACTAAAG AGGGGTTGTG GACCCAGGAA 
TTCAAGTTGT GTGAAAACCT GCAAGGAGAA TGCCCACCAC CCACCTCAGA GCTGAATTCT 
GTGGAGTACA AGTGTGGACA GGGATATGGG ATCGGTGCAG TGTGCTCCCC ATCATGTGTA 
ATCCCCCCTA GTGACCCTGT GATACTACCA GAGAATGTGA CTACTGACAC TTTGGAGCAC 
TGGATGGAAC CTGTCAAAGT CCAGAGTATC GTGTGTACCG GCAGGCGCCA GTGGCACCCA 
GATCCCTCTC TGGTGCACTG CATCCAGTCA TGTGAGCCTT TCCAAGCAGA TGGTTGGTGT 
GACACTATTA ATAACCGGGC CTACTGCAAC TATGACGGGG GAGACTGCTG CTCTTCCACA 
CTCTCTTCCA AGAAGGTCAT TCCATTTGCT GCTGATTGTG ACTTGGATGA ATGCACCTGT 
CGAGACCCCA AGGCAGAAGA AAACCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001078845.1
CDS	1..5370
Translation	MMCWKVLRISLVVLTGWALSTTNSEQGWTRKKFLAQKEHLNQVLLEGERCWLGYKVRRPRTAPQHHLFGVYPSRLENYPRSYPVGTQQQTLYAEWSQRSPERQAPSGIPRDLTENPAGARRESQQPAAPWVGDGPIGQSQLLRNDDTYLGEGGSKEALGEPAIQGSSEIAVSTIATFAYQKGPTAETQRKGQSKSRLPRQVQKRQAEDMTGDPQNTPQGFQLWPKEPLKHGDSDSPLEGTIQNGGGASLRRVETFNSQGGLPVLYFTGKRERLLLRPEVLAEIPREAFTVEAWVRPEGGQSNPAIIAGVFDNCSHTVNDKGWALGIRSGKDKGWRDARFFFSLRTDRMKKATIVTGHSRYQPGMWTHVAATYDGQHTALYVDGTRVASSRDQFGPLNSPFMASCRSLILGGDSSEDGHYFRGYLGTLVIWSTALSQTHLQHSPLHPSAADELTTLILTATFDPLMEQWAPFRDDLYPRLEVLQDSESQPEILSPLQPPPCGQTACDNVELISQYNKHGPLRREKVIRYQVVNIYDDEGLHPIVSDHQIRRQHEALNQAFSRYNISWQLSVHRVHNSTLRHRVVLVNCEPSKIGNDHCDPECEHPLTGFDGGDCRLQGRCYSWNRRDGLCHVECNNMLNDFDDGDCCDPEVTDVRKTCFDPDSPKRAYMSVKELKEALQLNSTHFLNVYFASSVREDLAGSATWPWDKEALSHLGGVVLNPTYYGMLGHTNIMIHEVGHVLGLYHVFKGVSERESCDDPCRETVPSMETGDLCADTAPTPKSKLCRDPEPANDTCGFTLFPGAPFNNYMSYTDDECTDNFTPNQVARMHCYLDLVYQQWSESQKPTPIPIPPMVIGQTHKSLTIHWLPPISGVVYDRAPDSMCSACTEDGTFRQYVYKASSGRVCDSSGYWTPEEAVGPPDVDQPCEPSLQAWSPELHLNHMNMTVPCPAEGCSLELLFQYPVQADTLTLWVTYLSMNSSKAFFDIEILLEHKKSMHLGPLNTFCDIPLTIKLHMDEKVMGAKVYTFDERMEIDAALLTSRPNSSLCSGCKPMSYQVLREPPFSSGLPMVVTHPHRKFTDTEVIPGQMYQYQVQAEAVGELGEASPPLSHIHGGPYCGDGKVERSIGEMCDDGDLLNGDGCSRACELEEGFNCAGEPSLCYRYEGDGVCEDFEKESIITDCGLHTPEGYLDQWASQAYSYHEDKEKCPVSLVTGEPHSMICTSWHPDSSPYHSFPGWFPCVFSLKKDQDAGSEQAKDSLQKDNEIWLEVCFNRPGVAVAIYIFLASDGVTPGEHQQATVTLYLIDVSGSNHSLGTYGLSCQQNPLVINVSHHVNVYPQHTSSMLLNFSSPLVGISAVALRTSSQTSSSAPSNCIPEQNYEGKSCAQRPCGEQGRCAPLLLDHTDMVNCTSSSPGHMECAITCQRGYVLQTSSGQYLRNMQKEILLTCSSGHWDKDVICKRLDCGVPDSSLVNYANFSCLEGTDFLKQCSISCVPPAKLQGLNPWLTCLEDGLWSLPEVYCKLECEAPPVIPNANLLLPHFLEGNHDVGTICKYECKPGYYVKETSGSQGKNKFLKIQCLDDGSWEQGSCVPVVCEPPPPVFEGMYECTDGFKLDSQCVLNCNQETEGIPILCTKEGLWTQEFKLCENLQGECPPPTSELNSVEYKCGQGYGIGAVCSPSCVIPPSDPVILPENVTTDTLEHWMEPVKVQSIVCTGRRQWHPDPSLVHCIQSCEPFQADGWCDTINNRAYCNYDGGDCCSSTLSSKKVIPFAADCDLDECTCRDPKAEENQ

Target ORF information:

RefSeq Version	NM_001085376.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus pappalysin 2 (Pappa2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001085376.2

ORF Insert Sequence:

ATGATGTGTT GGAAGGTCCT GAGAATAAGC CTGGTGGTCC TGACTGGGTG GGCACTCAGT 
ACCACCAACT CAGAGCAGGG CTGGACACGC AAGAAATTCC TGGCCCAGAA GGAACACCTG 
AACCAAGTGC TGTTGGAAGG GGAACGCTGT TGGCTGGGGT ATAAGGTTCG AAGACCCAGG 
ACTGCTCCAC AGCACCATCT CTTTGGGGTC TACCCAAGCC GCCTCGAAAA CTACCCAAGG 
TCCTATCCTG TGGGGACACA GCAGCAAACC CTGTATGCAG AATGGAGTCA GCGAAGCCCA 
GAGAGACAGG CTCCGAGTGG TATTCCCCGA GACCTGACTG AAAACCCAGC AGGAGCAAGG 
AGAGAGTCTC AGCAGCCAGC TGCCCCGTGG GTAGGGGATG GTCCTATTGG GCAATCGCAG 
CTGCTGAGAA ATGATGACAC TTATCTTGGC GAGGGAGGAT CTAAGGAGGC TCTAGGTGAG 
CCTGCGATTC AGGGAAGCTC AGAAATTGCT GTCTCCACCA TTGCTACCTT CGCATATCAG 
AAGGGACCCA CAGCAGAGAC CCAGAGGAAG GGCCAGTCCA AGTCTAGGCT TCCTCGCCAA 
GTGCAGAAGA GGCAGGCAGA AGACATGACT GGAGACCCCC AGAATACCCC TCAAGGTTTC 
CAGCTTTGGC CTAAAGAGCC ACTTAAGCAT GGAGATAGTG ACAGTCCATT GGAGGGCACC 
ATCCAAAATG GTGGAGGGGC CTCTCTCCGG AGAGTAGAGA CCTTTAACTC CCAAGGAGGA 
TTGCCTGTAC TGTATTTCAC TGGGAAGAGG GAGCGGCTGT TGCTGCGTCC AGAAGTGCTG 
GCTGAGATTC CCCGGGAGGC GTTCACAGTG GAAGCCTGGG TGAGACCAGA GGGAGGACAG 
AGCAACCCAG CCATCATTGC AGGTGTGTTT GATAACTGCT CCCACACAGT CAATGACAAG 
GGCTGGGCCC TGGGGATCCG CTCAGGGAAG GACAAGGGGT GGAGAGATGC TCGCTTCTTC 
TTTTCCCTCC GCACTGACCG GATGAAGAAA GCCACTATTG TGACTGGCCA CAGCCGTTAC 
CAGCCAGGCA TGTGGACTCA TGTAGCAGCC ACTTACGATG GACAGCATAC AGCTCTGTAT 
GTGGATGGCA CTCGAGTGGC TAGTAGTCGT GACCAGTTTG GACCGCTAAA TAGCCCTTTC 
ATGGCGTCTT GCCGCTCTTT AATCCTGGGA GGGGACAGCT CCGAGGATGG ACATTATTTC 
CGTGGTTACC TGGGCACACT GGTAATCTGG TCGACCGCCC TTTCACAAAC CCACCTCCAG 
CACAGTCCCC TGCATCCAAG TGCAGCAGAT GAGTTGACCA CCCTGATCCT GACAGCCACT 
TTCGATCCTC TGATGGAACA GTGGGCTCCA TTTAGAGATG ACTTATATCC CCGGCTGGAG 
GTTCTCCAGG ACTCTGAGTC ACAGCCTGAG ATTCTGTCAC CTTTACAACC CCCACCTTGT 
GGGCAAACAG CTTGTGACAA TGTGGAACTC ATCTCCCAGT ACAATAAGCA TGGGCCCCTT 
CGTAGAGAGA AAGTGATCCG CTACCAGGTG GTAAACATCT ATGATGATGA GGGTCTGCAT 
CCCATTGTGA GTGATCATCA GATCCGCCGT CAGCATGAGG CCCTCAACCA GGCCTTCAGC 
CGCTACAATA TCAGCTGGCA GCTGAGTGTC CACCGAGTCC ACAACTCCAC CCTGCGCCAC 
CGGGTTGTGC TTGTTAATTG TGAGCCCAGC AAGATTGGCA ATGACCACTG TGACCCTGAA 
TGTGAGCATC CACTCACCGG CTTCGATGGG GGCGATTGCC GCCTGCAGGG ACGCTGCTAC 
TCCTGGAATC GCAGGGATGG CCTCTGTCAC GTGGAGTGCA ACAACATGCT GAATGACTTC 
GATGATGGAG ACTGCTGCGA TCCGGAAGTG ACTGATGTCC GAAAGACTTG CTTTGATCCT 
GACTCACCCA AGAGGGCATA CATGAGTGTG AAGGAACTAA AGGAGGCCTT GCAGCTCAAC 
AGCACTCACT TCCTTAATGT CTACTTCGCC AGCTCAGTCC GGGAAGACCT TGCAGGTTCT 
GCCACATGGC CCTGGGACAA GGAAGCTCTC AGTCACCTGG GTGGTGTTGT CCTCAACCCA 
ACCTATTATG GCATGCTTGG CCACACCAAC ATCATGATCC ATGAGGTGGG ACATGTCCTG 
GGACTCTACC ATGTATTCAA AGGAGTCAGT GAAAGAGAAT CCTGTGATGA TCCCTGTAGA 
GAGACAGTGC CATCCATGGA GACAGGAGAC CTGTGTGCTG ACACTGCTCC CACACCCAAG 
AGCAAGCTGT GTCGGGACCC AGAGCCAGCT AATGACACCT GTGGCTTCAC CCTCTTCCCA 
GGGGCTCCGT TCAATAACTA CATGAGCTAC ACAGATGATG AGTGCACTGA CAACTTCACC 
CCTAACCAAG TGGCCCGGAT GCATTGCTAC TTGGACCTCG TATACCAGCA ATGGAGTGAA 
AGCCAAAAAC CTACCCCCAT ACCCATACCA CCCATGGTTA TTGGGCAAAC CCACAAGTCC 
CTTACTATCC ATTGGCTGCC TCCAATCAGT GGAGTTGTGT ATGATAGGGC CCCTGACAGC 
ATGTGTAGTG CCTGCACTGA AGATGGGACA TTCCGTCAGT ATGTATACAA GGCATCCTCT 
GGGCGGGTGT GTGACTCTTC AGGTTACTGG ACTCCAGAGG AGGCTGTGGG GCCTCCTGAT 
GTCGATCAAC CCTGTGAGCC TAGCTTGCAG GCCTGGAGTC CTGAGCTCCA CCTAAACCAC 
ATGAATATGA CCGTGCCCTG CCCAGCAGAA GGCTGTAGCT TGGAGTTGCT TTTTCAATAC 
CCGGTCCAGG CGGATACCCT CACCCTCTGG GTCACTTACC TCTCCATGAA CTCTTCCAAG 
GCATTCTTTG ACATAGAGAT CTTGCTAGAA CACAAAAAAT CTATGCACCT TGGACCTTTA 
AACACTTTTT GTGACATACC GCTCACCATC AAACTCCACA TGGATGAGAA GGTCATGGGA 
GCGAAAGTCT ACACCTTTGA TGAACGAATG GAAATTGATG CTGCTCTCCT GACCTCTCGG 
CCCAATAGTT CCTTGTGTTC TGGATGCAAG CCTATGAGTT ACCAGGTTCT TCGAGAGCCT 
CCTTTTTCCA GTGGCTTGCC CATGGTGGTG ACACATCCAC ACAGAAAGTT CACAGATACG 
GAGGTCATCC CTGGGCAGAT GTATCAGTAC CAAGTTCAAG CTGAAGCTGT AGGGGAACTC 
GGAGAAGCTT CACCTCCTCT GAGCCACATT CACGGAGGCC CTTACTGTGG AGATGGGAAG 
GTAGAAAGGA GTATCGGAGA GATGTGTGAT GATGGAGACC TGTTGAATGG AGATGGGTGC 
TCAAGAGCAT GTGAACTGGA GGAAGGCTTC AATTGTGCAG GGGAGCCAAG CCTTTGCTAC 
AGGTATGAGG GAGATGGGGT TTGTGAAGAT TTTGAGAAGG AAAGCATCAT CACAGACTGT 
GGCCTCCACA CTCCTGAAGG ATATTTGGAT CAGTGGGCTA GCCAGGCTTA TTCCTACCAT 
GAAGACAAGG AGAAATGTCC TGTTTCCCTG GTAACAGGAG AACCTCATTC AATGATCTGT 
ACTTCATGGC ACCCGGATTC ATCCCCATAT CATTCCTTTC CTGGCTGGTT TCCCTGTGTC 
TTCAGTCTGA AGAAAGACCA GGATGCAGGC AGTGAGCAGG CTAAAGATAG CCTGCAGAAG 
GACAATGAAA TTTGGCTTGA AGTGTGTTTC AATAGGCCAG GGGTTGCAGT GGCAATTTAC 
ATTTTCTTGG CATCGGATGG CGTGACCCCT GGGGAGCATC AGCAGGCAAC AGTGACCCTT 
TACCTGATTG ATGTCAGTGG AAGCAACCAC TCTCTTGGAA CCTATGGACT GTCGTGTCAA 
CAAAATCCAC TGGTTATCAA TGTGAGCCAT CATGTGAATG TCTACCCCCA ACATACCTCC 
TCCATGCTGC TGAATTTTTC ATCCCCACTG GTGGGCATCT CAGCAGTGGC TCTAAGGACA 
TCCTCCCAGA CCAGTTCTTC AGCTCCCAGT AACTGCATCC CAGAGCAAAA TTATGAGGGA 
AAGAGCTGTG CTCAACGACC TTGTGGAGAA CAGGGAAGAT GTGCGCCATT GCTACTCGAT 
CATACGGACA TGGTGAATTG TACCTCCAGT AGCCCAGGTC ACATGGAGTG CGCTATCACT 
TGTCAAAGGG GGTATGTCCT TCAGACCAGC AGTGGGCAGT ACCTGAGGAA CATGCAGAAA 
GAGATTCTCC TTACTTGTTC CTCTGGCCAC TGGGACAAGG ATGTGATCTG CAAGCGTCTT 
GACTGTGGTG TCCCAGACTC CTCCTTGGTG AATTATGCAA ACTTCTCCTG CTTGGAGGGT 
ACAGACTTTC TGAAACAATG TTCCATCTCG TGTGTCCCAC CGGCTAAGCT CCAAGGACTG 
AACCCATGGC TGACATGTCT TGAAGATGGA CTCTGGTCTC TCCCTGAAGT CTACTGCAAG 
TTGGAATGTG AAGCTCCTCC AGTTATTCCC AATGCCAATC TGCTCCTGCC ACATTTCCTG 
GAAGGCAACC ATGATGTGGG CACCATCTGC AAATATGAGT GCAAACCAGG CTACTATGTG 
AAGGAGACTT CAGGAAGTCA AGGCAAGAAC AAGTTCCTGA AGATCCAGTG CCTGGATGAT 
GGAAGCTGGG AACAAGGGAG CTGCGTCCCC GTGGTTTGTG AGCCTCCTCC TCCGGTGTTT 
GAAGGCATGT ACGAATGTAC TGATGGCTTC AAGCTGGACA GCCAGTGTGT GCTCAACTGT 
AACCAGGAAA CGGAAGGGAT TCCCATCCTC TGCACTAAAG AGGGGTTGTG GACCCAGGAA 
TTCAAGTTGT GTGAAAACCT GCAAGGAGAA TGCCCACCAC CCACCTCAGA GCTGAATTCT 
GTGGAGTACA AGTGTGGACA GGGATATGGG ATCGGTGCAG TGTGCTCCCC ATCATGTGTA 
ATCCCCCCTA GTGACCCTGT GATACTACCA GAGAATGTGA CTACTGACAC TTTGGAGCAC 
TGGATGGAAC CTGTCAAAGT CCAGAGTATC GTGTGTACCG GCAGGCGCCA GTGGCACCCA 
GATCCCTCTC TGGTGCACTG CATCCAGTCA TGTGAGCCTT TCCAAGCAGA TGGTTGGTGT 
GACACTATTA ATAACCGGGC CTACTGCAAC TATGACGGGG GAGACTGCTG CTCTTCCACA 
CTCTCTTCCA AGAAGGTCAT TCCATTTGCT GCTGATTGTG ACTTGGATGA ATGCACCTGT 
CGAGACCCCA AGGCAGAAGA AAACCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pappa2 ( XM_011238806.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011237108.1 Mus musculus pappalysin 2 (Pappa2), transcript variant X2, mRNA.

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CloneID	OMu02923
Clone ID Related Accession (Same CDS sequence)	NM_001085376.3 , XM_006496824.4 , XM_011238806.2 , XM_006496823.3 , XM_006496824.3 , NM_001085376.2
Accession Version	XM_011238806.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5370bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	pappalysin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011238806.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGTT GGAAGGTCCT GAGAATAAGC CTGGTGGTCC TGACTGGGTG GGCACTCAGT 
ACCACCAACT CAGAGCAGGG CTGGACACGC AAGAAATTCC TGGCCCAGAA GGAACACCTG 
AACCAAGTGC TGTTGGAAGG GGAACGCTGT TGGCTGGGGT ATAAGGTTCG AAGACCCAGG 
ACTGCTCCAC AGCACCATCT CTTTGGGGTC TACCCAAGCC GCCTCGAAAA CTACCCAAGG 
TCCTATCCTG TGGGGACACA GCAGCAAACC CTGTATGCAG AATGGAGTCA GCGAAGCCCA 
GAGAGACAGG CTCCGAGTGG TATTCCCCGA GACCTGACTG AAAACCCAGC AGGAGCAAGG 
AGAGAGTCTC AGCAGCCAGC TGCCCCGTGG GTAGGGGATG GTCCTATTGG GCAATCGCAG 
CTGCTGAGAA ATGATGACAC TTATCTTGGC GAGGGAGGAT CTAAGGAGGC TCTAGGTGAG 
CCTGCGATTC AGGGAAGCTC AGAAATTGCT GTCTCCACCA TTGCTACCTT CGCATATCAG 
AAGGGACCCA CAGCAGAGAC CCAGAGGAAG GGCCAGTCCA AGTCTAGGCT TCCTCGCCAA 
GTGCAGAAGA GGCAGGCAGA AGACATGACT GGAGACCCCC AGAATACCCC TCAAGGTTTC 
CAGCTTTGGC CTAAAGAGCC ACTTAAGCAT GGAGATAGTG ACAGTCCATT GGAGGGCACC 
ATCCAAAATG GTGGAGGGGC CTCTCTCCGG AGAGTAGAGA CCTTTAACTC CCAAGGAGGA 
TTGCCTGTAC TGTATTTCAC TGGGAAGAGG GAGCGGCTGT TGCTGCGTCC AGAAGTGCTG 
GCTGAGATTC CCCGGGAGGC GTTCACAGTG GAAGCCTGGG TGAGACCAGA GGGAGGACAG 
AGCAACCCAG CCATCATTGC AGGTGTGTTT GATAACTGCT CCCACACAGT CAATGACAAG 
GGCTGGGCCC TGGGGATCCG CTCAGGGAAG GACAAGGGGT GGAGAGATGC TCGCTTCTTC 
TTTTCCCTCC GCACTGACCG GATGAAGAAA GCCACTATTG TGACTGGCCA CAGCCGTTAC 
CAGCCAGGCA TGTGGACTCA TGTAGCAGCC ACTTACGATG GACAGCATAC AGCTCTGTAT 
GTGGATGGCA CTCGAGTGGC TAGTAGTCGT GACCAGTTTG GACCGCTAAA TAGCCCTTTC 
ATGGCGTCTT GCCGCTCTTT AATCCTGGGA GGGGACAGCT CCGAGGATGG ACATTATTTC 
CGTGGTTACC TGGGCACACT GGTAATCTGG TCGACCGCCC TTTCACAAAC CCACCTCCAG 
CACAGTCCCC TGCATCCAAG TGCAGCAGAT GAGTTGACCA CCCTGATCCT GACAGCCACT 
TTCGATCCTC TGATGGAACA GTGGGCTCCA TTTAGAGATG ACTTATATCC CCGGCTGGAG 
GTTCTCCAGG ACTCTGAGTC ACAGCCTGAG ATTCTGTCAC CTTTACAACC CCCACCTTGT 
GGGCAAACAG CTTGTGACAA TGTGGAACTC ATCTCCCAGT ACAATAAGCA TGGGCCCCTT 
CGTAGAGAGA AAGTGATCCG CTACCAGGTG GTAAACATCT ATGATGATGA GGGTCTGCAT 
CCCATTGTGA GTGATCATCA GATCCGCCGT CAGCATGAGG CCCTCAACCA GGCCTTCAGC 
CGCTACAATA TCAGCTGGCA GCTGAGTGTC CACCGAGTCC ACAACTCCAC CCTGCGCCAC 
CGGGTTGTGC TTGTTAATTG TGAGCCCAGC AAGATTGGCA ATGACCACTG TGACCCTGAA 
TGTGAGCATC CACTCACCGG CTTCGATGGG GGCGATTGCC GCCTGCAGGG ACGCTGCTAC 
TCCTGGAATC GCAGGGATGG CCTCTGTCAC GTGGAGTGCA ACAACATGCT GAATGACTTC 
GATGATGGAG ACTGCTGCGA TCCGGAAGTG ACTGATGTCC GAAAGACTTG CTTTGATCCT 
GACTCACCCA AGAGGGCATA CATGAGTGTG AAGGAACTAA AGGAGGCCTT GCAGCTCAAC 
AGCACTCACT TCCTTAATGT CTACTTCGCC AGCTCAGTCC GGGAAGACCT TGCAGGTTCT 
GCCACATGGC CCTGGGACAA GGAAGCTCTC AGTCACCTGG GTGGTGTTGT CCTCAACCCA 
ACCTATTATG GCATGCTTGG CCACACCAAC ATCATGATCC ATGAGGTGGG ACATGTCCTG 
GGACTCTACC ATGTATTCAA AGGAGTCAGT GAAAGAGAAT CCTGTGATGA TCCCTGTAGA 
GAGACAGTGC CATCCATGGA GACAGGAGAC CTGTGTGCTG ACACTGCTCC CACACCCAAG 
AGCAAGCTGT GTCGGGACCC AGAGCCAGCT AATGACACCT GTGGCTTCAC CCTCTTCCCA 
GGGGCTCCGT TCAATAACTA CATGAGCTAC ACAGATGATG AGTGCACTGA CAACTTCACC 
CCTAACCAAG TGGCCCGGAT GCATTGCTAC TTGGACCTCG TATACCAGCA ATGGAGTGAA 
AGCCAAAAAC CTACCCCCAT ACCCATACCA CCCATGGTTA TTGGGCAAAC CCACAAGTCC 
CTTACTATCC ATTGGCTGCC TCCAATCAGT GGAGTTGTGT ATGATAGGGC CCCTGACAGC 
ATGTGTAGTG CCTGCACTGA AGATGGGACA TTCCGTCAGT ATGTATACAA GGCATCCTCT 
GGGCGGGTGT GTGACTCTTC AGGTTACTGG ACTCCAGAGG AGGCTGTGGG GCCTCCTGAT 
GTCGATCAAC CCTGTGAGCC TAGCTTGCAG GCCTGGAGTC CTGAGCTCCA CCTAAACCAC 
ATGAATATGA CCGTGCCCTG CCCAGCAGAA GGCTGTAGCT TGGAGTTGCT TTTTCAATAC 
CCGGTCCAGG CGGATACCCT CACCCTCTGG GTCACTTACC TCTCCATGAA CTCTTCCAAG 
GCATTCTTTG ACATAGAGAT CTTGCTAGAA CACAAAAAAT CTATGCACCT TGGACCTTTA 
AACACTTTTT GTGACATACC GCTCACCATC AAACTCCACA TGGATGAGAA GGTCATGGGA 
GCGAAAGTCT ACACCTTTGA TGAACGAATG GAAATTGATG CTGCTCTCCT GACCTCTCGG 
CCCAATAGTT CCTTGTGTTC TGGATGCAAG CCTATGAGTT ACCAGGTTCT TCGAGAGCCT 
CCTTTTTCCA GTGGCTTGCC CATGGTGGTG ACACATCCAC ACAGAAAGTT CACAGATACG 
GAGGTCATCC CTGGGCAGAT GTATCAGTAC CAAGTTCAAG CTGAAGCTGT AGGGGAACTC 
GGAGAAGCTT CACCTCCTCT GAGCCACATT CACGGAGGCC CTTACTGTGG AGATGGGAAG 
GTAGAAAGGA GTATCGGAGA GATGTGTGAT GATGGAGACC TGTTGAATGG AGATGGGTGC 
TCAAGAGCAT GTGAACTGGA GGAAGGCTTC AATTGTGCAG GGGAGCCAAG CCTTTGCTAC 
AGGTATGAGG GAGATGGGGT TTGTGAAGAT TTTGAGAAGG AAAGCATCAT CACAGACTGT 
GGCCTCCACA CTCCTGAAGG ATATTTGGAT CAGTGGGCTA GCCAGGCTTA TTCCTACCAT 
GAAGACAAGG AGAAATGTCC TGTTTCCCTG GTAACAGGAG AACCTCATTC AATGATCTGT 
ACTTCATGGC ACCCGGATTC ATCCCCATAT CATTCCTTTC CTGGCTGGTT TCCCTGTGTC 
TTCAGTCTGA AGAAAGACCA GGATGCAGGC AGTGAGCAGG CTAAAGATAG CCTGCAGAAG 
GACAATGAAA TTTGGCTTGA AGTGTGTTTC AATAGGCCAG GGGTTGCAGT GGCAATTTAC 
ATTTTCTTGG CATCGGATGG CGTGACCCCT GGGGAGCATC AGCAGGCAAC AGTGACCCTT 
TACCTGATTG ATGTCAGTGG AAGCAACCAC TCTCTTGGAA CCTATGGACT GTCGTGTCAA 
CAAAATCCAC TGGTTATCAA TGTGAGCCAT CATGTGAATG TCTACCCCCA ACATACCTCC 
TCCATGCTGC TGAATTTTTC ATCCCCACTG GTGGGCATCT CAGCAGTGGC TCTAAGGACA 
TCCTCCCAGA CCAGTTCTTC AGCTCCCAGT AACTGCATCC CAGAGCAAAA TTATGAGGGA 
AAGAGCTGTG CTCAACGACC TTGTGGAGAA CAGGGAAGAT GTGCGCCATT GCTACTCGAT 
CATACGGACA TGGTGAATTG TACCTCCAGT AGCCCAGGTC ACATGGAGTG CGCTATCACT 
TGTCAAAGGG GGTATGTCCT TCAGACCAGC AGTGGGCAGT ACCTGAGGAA CATGCAGAAA 
GAGATTCTCC TTACTTGTTC CTCTGGCCAC TGGGACAAGG ATGTGATCTG CAAGCGTCTT 
GACTGTGGTG TCCCAGACTC CTCCTTGGTG AATTATGCAA ACTTCTCCTG CTTGGAGGGT 
ACAGACTTTC TGAAACAATG TTCCATCTCG TGTGTCCCAC CGGCTAAGCT CCAAGGACTG 
AACCCATGGC TGACATGTCT TGAAGATGGA CTCTGGTCTC TCCCTGAAGT CTACTGCAAG 
TTGGAATGTG AAGCTCCTCC AGTTATTCCC AATGCCAATC TGCTCCTGCC ACATTTCCTG 
GAAGGCAACC ATGATGTGGG CACCATCTGC AAATATGAGT GCAAACCAGG CTACTATGTG 
AAGGAGACTT CAGGAAGTCA AGGCAAGAAC AAGTTCCTGA AGATCCAGTG CCTGGATGAT 
GGAAGCTGGG AACAAGGGAG CTGCGTCCCC GTGGTTTGTG AGCCTCCTCC TCCGGTGTTT 
GAAGGCATGT ACGAATGTAC TGATGGCTTC AAGCTGGACA GCCAGTGTGT GCTCAACTGT 
AACCAGGAAA CGGAAGGGAT TCCCATCCTC TGCACTAAAG AGGGGTTGTG GACCCAGGAA 
TTCAAGTTGT GTGAAAACCT GCAAGGAGAA TGCCCACCAC CCACCTCAGA GCTGAATTCT 
GTGGAGTACA AGTGTGGACA GGGATATGGG ATCGGTGCAG TGTGCTCCCC ATCATGTGTA 
ATCCCCCCTA GTGACCCTGT GATACTACCA GAGAATGTGA CTACTGACAC TTTGGAGCAC 
TGGATGGAAC CTGTCAAAGT CCAGAGTATC GTGTGTACCG GCAGGCGCCA GTGGCACCCA 
GATCCCTCTC TGGTGCACTG CATCCAGTCA TGTGAGCCTT TCCAAGCAGA TGGTTGGTGT 
GACACTATTA ATAACCGGGC CTACTGCAAC TATGACGGGG GAGACTGCTG CTCTTCCACA 
CTCTCTTCCA AGAAGGTCAT TCCATTTGCT GCTGATTGTG ACTTGGATGA ATGCACCTGT 
CGAGACCCCA AGGCAGAAGA AAACCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011237108.1
CDS	959..6328
Translation	MMCWKVLRISLVVLTGWALSTTNSEQGWTRKKFLAQKEHLNQVLLEGERCWLGYKVRRPRTAPQHHLFGVYPSRLENYPRSYPVGTQQQTLYAEWSQRSPERQAPSGIPRDLTENPAGARRESQQPAAPWVGDGPIGQSQLLRNDDTYLGEGGSKEALGEPAIQGSSEIAVSTIATFAYQKGPTAETQRKGQSKSRLPRQVQKRQAEDMTGDPQNTPQGFQLWPKEPLKHGDSDSPLEGTIQNGGGASLRRVETFNSQGGLPVLYFTGKRERLLLRPEVLAEIPREAFTVEAWVRPEGGQSNPAIIAGVFDNCSHTVNDKGWALGIRSGKDKGWRDARFFFSLRTDRMKKATIVTGHSRYQPGMWTHVAATYDGQHTALYVDGTRVASSRDQFGPLNSPFMASCRSLILGGDSSEDGHYFRGYLGTLVIWSTALSQTHLQHSPLHPSAADELTTLILTATFDPLMEQWAPFRDDLYPRLEVLQDSESQPEILSPLQPPPCGQTACDNVELISQYNKHGPLRREKVIRYQVVNIYDDEGLHPIVSDHQIRRQHEALNQAFSRYNISWQLSVHRVHNSTLRHRVVLVNCEPSKIGNDHCDPECEHPLTGFDGGDCRLQGRCYSWNRRDGLCHVECNNMLNDFDDGDCCDPEVTDVRKTCFDPDSPKRAYMSVKELKEALQLNSTHFLNVYFASSVREDLAGSATWPWDKEALSHLGGVVLNPTYYGMLGHTNIMIHEVGHVLGLYHVFKGVSERESCDDPCRETVPSMETGDLCADTAPTPKSKLCRDPEPANDTCGFTLFPGAPFNNYMSYTDDECTDNFTPNQVARMHCYLDLVYQQWSESQKPTPIPIPPMVIGQTHKSLTIHWLPPISGVVYDRAPDSMCSACTEDGTFRQYVYKASSGRVCDSSGYWTPEEAVGPPDVDQPCEPSLQAWSPELHLNHMNMTVPCPAEGCSLELLFQYPVQADTLTLWVTYLSMNSSKAFFDIEILLEHKKSMHLGPLNTFCDIPLTIKLHMDEKVMGAKVYTFDERMEIDAALLTSRPNSSLCSGCKPMSYQVLREPPFSSGLPMVVTHPHRKFTDTEVIPGQMYQYQVQAEAVGELGEASPPLSHIHGGPYCGDGKVERSIGEMCDDGDLLNGDGCSRACELEEGFNCAGEPSLCYRYEGDGVCEDFEKESIITDCGLHTPEGYLDQWASQAYSYHEDKEKCPVSLVTGEPHSMICTSWHPDSSPYHSFPGWFPCVFSLKKDQDAGSEQAKDSLQKDNEIWLEVCFNRPGVAVAIYIFLASDGVTPGEHQQATVTLYLIDVSGSNHSLGTYGLSCQQNPLVINVSHHVNVYPQHTSSMLLNFSSPLVGISAVALRTSSQTSSSAPSNCIPEQNYEGKSCAQRPCGEQGRCAPLLLDHTDMVNCTSSSPGHMECAITCQRGYVLQTSSGQYLRNMQKEILLTCSSGHWDKDVICKRLDCGVPDSSLVNYANFSCLEGTDFLKQCSISCVPPAKLQGLNPWLTCLEDGLWSLPEVYCKLECEAPPVIPNANLLLPHFLEGNHDVGTICKYECKPGYYVKETSGSQGKNKFLKIQCLDDGSWEQGSCVPVVCEPPPPVFEGMYECTDGFKLDSQCVLNCNQETEGIPILCTKEGLWTQEFKLCENLQGECPPPTSELNSVEYKCGQGYGIGAVCSPSCVIPPSDPVILPENVTTDTLEHWMEPVKVQSIVCTGRRQWHPDPSLVHCIQSCEPFQADGWCDTINNRAYCNYDGGDCCSSTLSSKKVIPFAADCDLDECTCRDPKAEENQ

Target ORF information:

RefSeq Version	XM_011238806.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus pappalysin 2 (Pappa2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238806.2

ORF Insert Sequence:

ATGATGTGTT GGAAGGTCCT GAGAATAAGC CTGGTGGTCC TGACTGGGTG GGCACTCAGT 
ACCACCAACT CAGAGCAGGG CTGGACACGC AAGAAATTCC TGGCCCAGAA GGAACACCTG 
AACCAAGTGC TGTTGGAAGG GGAACGCTGT TGGCTGGGGT ATAAGGTTCG AAGACCCAGG 
ACTGCTCCAC AGCACCATCT CTTTGGGGTC TACCCAAGCC GCCTCGAAAA CTACCCAAGG 
TCCTATCCTG TGGGGACACA GCAGCAAACC CTGTATGCAG AATGGAGTCA GCGAAGCCCA 
GAGAGACAGG CTCCGAGTGG TATTCCCCGA GACCTGACTG AAAACCCAGC AGGAGCAAGG 
AGAGAGTCTC AGCAGCCAGC TGCCCCGTGG GTAGGGGATG GTCCTATTGG GCAATCGCAG 
CTGCTGAGAA ATGATGACAC TTATCTTGGC GAGGGAGGAT CTAAGGAGGC TCTAGGTGAG 
CCTGCGATTC AGGGAAGCTC AGAAATTGCT GTCTCCACCA TTGCTACCTT CGCATATCAG 
AAGGGACCCA CAGCAGAGAC CCAGAGGAAG GGCCAGTCCA AGTCTAGGCT TCCTCGCCAA 
GTGCAGAAGA GGCAGGCAGA AGACATGACT GGAGACCCCC AGAATACCCC TCAAGGTTTC 
CAGCTTTGGC CTAAAGAGCC ACTTAAGCAT GGAGATAGTG ACAGTCCATT GGAGGGCACC 
ATCCAAAATG GTGGAGGGGC CTCTCTCCGG AGAGTAGAGA CCTTTAACTC CCAAGGAGGA 
TTGCCTGTAC TGTATTTCAC TGGGAAGAGG GAGCGGCTGT TGCTGCGTCC AGAAGTGCTG 
GCTGAGATTC CCCGGGAGGC GTTCACAGTG GAAGCCTGGG TGAGACCAGA GGGAGGACAG 
AGCAACCCAG CCATCATTGC AGGTGTGTTT GATAACTGCT CCCACACAGT CAATGACAAG 
GGCTGGGCCC TGGGGATCCG CTCAGGGAAG GACAAGGGGT GGAGAGATGC TCGCTTCTTC 
TTTTCCCTCC GCACTGACCG GATGAAGAAA GCCACTATTG TGACTGGCCA CAGCCGTTAC 
CAGCCAGGCA TGTGGACTCA TGTAGCAGCC ACTTACGATG GACAGCATAC AGCTCTGTAT 
GTGGATGGCA CTCGAGTGGC TAGTAGTCGT GACCAGTTTG GACCGCTAAA TAGCCCTTTC 
ATGGCGTCTT GCCGCTCTTT AATCCTGGGA GGGGACAGCT CCGAGGATGG ACATTATTTC 
CGTGGTTACC TGGGCACACT GGTAATCTGG TCGACCGCCC TTTCACAAAC CCACCTCCAG 
CACAGTCCCC TGCATCCAAG TGCAGCAGAT GAGTTGACCA CCCTGATCCT GACAGCCACT 
TTCGATCCTC TGATGGAACA GTGGGCTCCA TTTAGAGATG ACTTATATCC CCGGCTGGAG 
GTTCTCCAGG ACTCTGAGTC ACAGCCTGAG ATTCTGTCAC CTTTACAACC CCCACCTTGT 
GGGCAAACAG CTTGTGACAA TGTGGAACTC ATCTCCCAGT ACAATAAGCA TGGGCCCCTT 
CGTAGAGAGA AAGTGATCCG CTACCAGGTG GTAAACATCT ATGATGATGA GGGTCTGCAT 
CCCATTGTGA GTGATCATCA GATCCGCCGT CAGCATGAGG CCCTCAACCA GGCCTTCAGC 
CGCTACAATA TCAGCTGGCA GCTGAGTGTC CACCGAGTCC ACAACTCCAC CCTGCGCCAC 
CGGGTTGTGC TTGTTAATTG TGAGCCCAGC AAGATTGGCA ATGACCACTG TGACCCTGAA 
TGTGAGCATC CACTCACCGG CTTCGATGGG GGCGATTGCC GCCTGCAGGG ACGCTGCTAC 
TCCTGGAATC GCAGGGATGG CCTCTGTCAC GTGGAGTGCA ACAACATGCT GAATGACTTC 
GATGATGGAG ACTGCTGCGA TCCGGAAGTG ACTGATGTCC GAAAGACTTG CTTTGATCCT 
GACTCACCCA AGAGGGCATA CATGAGTGTG AAGGAACTAA AGGAGGCCTT GCAGCTCAAC 
AGCACTCACT TCCTTAATGT CTACTTCGCC AGCTCAGTCC GGGAAGACCT TGCAGGTTCT 
GCCACATGGC CCTGGGACAA GGAAGCTCTC AGTCACCTGG GTGGTGTTGT CCTCAACCCA 
ACCTATTATG GCATGCTTGG CCACACCAAC ATCATGATCC ATGAGGTGGG ACATGTCCTG 
GGACTCTACC ATGTATTCAA AGGAGTCAGT GAAAGAGAAT CCTGTGATGA TCCCTGTAGA 
GAGACAGTGC CATCCATGGA GACAGGAGAC CTGTGTGCTG ACACTGCTCC CACACCCAAG 
AGCAAGCTGT GTCGGGACCC AGAGCCAGCT AATGACACCT GTGGCTTCAC CCTCTTCCCA 
GGGGCTCCGT TCAATAACTA CATGAGCTAC ACAGATGATG AGTGCACTGA CAACTTCACC 
CCTAACCAAG TGGCCCGGAT GCATTGCTAC TTGGACCTCG TATACCAGCA ATGGAGTGAA 
AGCCAAAAAC CTACCCCCAT ACCCATACCA CCCATGGTTA TTGGGCAAAC CCACAAGTCC 
CTTACTATCC ATTGGCTGCC TCCAATCAGT GGAGTTGTGT ATGATAGGGC CCCTGACAGC 
ATGTGTAGTG CCTGCACTGA AGATGGGACA TTCCGTCAGT ATGTATACAA GGCATCCTCT 
GGGCGGGTGT GTGACTCTTC AGGTTACTGG ACTCCAGAGG AGGCTGTGGG GCCTCCTGAT 
GTCGATCAAC CCTGTGAGCC TAGCTTGCAG GCCTGGAGTC CTGAGCTCCA CCTAAACCAC 
ATGAATATGA CCGTGCCCTG CCCAGCAGAA GGCTGTAGCT TGGAGTTGCT TTTTCAATAC 
CCGGTCCAGG CGGATACCCT CACCCTCTGG GTCACTTACC TCTCCATGAA CTCTTCCAAG 
GCATTCTTTG ACATAGAGAT CTTGCTAGAA CACAAAAAAT CTATGCACCT TGGACCTTTA 
AACACTTTTT GTGACATACC GCTCACCATC AAACTCCACA TGGATGAGAA GGTCATGGGA 
GCGAAAGTCT ACACCTTTGA TGAACGAATG GAAATTGATG CTGCTCTCCT GACCTCTCGG 
CCCAATAGTT CCTTGTGTTC TGGATGCAAG CCTATGAGTT ACCAGGTTCT TCGAGAGCCT 
CCTTTTTCCA GTGGCTTGCC CATGGTGGTG ACACATCCAC ACAGAAAGTT CACAGATACG 
GAGGTCATCC CTGGGCAGAT GTATCAGTAC CAAGTTCAAG CTGAAGCTGT AGGGGAACTC 
GGAGAAGCTT CACCTCCTCT GAGCCACATT CACGGAGGCC CTTACTGTGG AGATGGGAAG 
GTAGAAAGGA GTATCGGAGA GATGTGTGAT GATGGAGACC TGTTGAATGG AGATGGGTGC 
TCAAGAGCAT GTGAACTGGA GGAAGGCTTC AATTGTGCAG GGGAGCCAAG CCTTTGCTAC 
AGGTATGAGG GAGATGGGGT TTGTGAAGAT TTTGAGAAGG AAAGCATCAT CACAGACTGT 
GGCCTCCACA CTCCTGAAGG ATATTTGGAT CAGTGGGCTA GCCAGGCTTA TTCCTACCAT 
GAAGACAAGG AGAAATGTCC TGTTTCCCTG GTAACAGGAG AACCTCATTC AATGATCTGT 
ACTTCATGGC ACCCGGATTC ATCCCCATAT CATTCCTTTC CTGGCTGGTT TCCCTGTGTC 
TTCAGTCTGA AGAAAGACCA GGATGCAGGC AGTGAGCAGG CTAAAGATAG CCTGCAGAAG 
GACAATGAAA TTTGGCTTGA AGTGTGTTTC AATAGGCCAG GGGTTGCAGT GGCAATTTAC 
ATTTTCTTGG CATCGGATGG CGTGACCCCT GGGGAGCATC AGCAGGCAAC AGTGACCCTT 
TACCTGATTG ATGTCAGTGG AAGCAACCAC TCTCTTGGAA CCTATGGACT GTCGTGTCAA 
CAAAATCCAC TGGTTATCAA TGTGAGCCAT CATGTGAATG TCTACCCCCA ACATACCTCC 
TCCATGCTGC TGAATTTTTC ATCCCCACTG GTGGGCATCT CAGCAGTGGC TCTAAGGACA 
TCCTCCCAGA CCAGTTCTTC AGCTCCCAGT AACTGCATCC CAGAGCAAAA TTATGAGGGA 
AAGAGCTGTG CTCAACGACC TTGTGGAGAA CAGGGAAGAT GTGCGCCATT GCTACTCGAT 
CATACGGACA TGGTGAATTG TACCTCCAGT AGCCCAGGTC ACATGGAGTG CGCTATCACT 
TGTCAAAGGG GGTATGTCCT TCAGACCAGC AGTGGGCAGT ACCTGAGGAA CATGCAGAAA 
GAGATTCTCC TTACTTGTTC CTCTGGCCAC TGGGACAAGG ATGTGATCTG CAAGCGTCTT 
GACTGTGGTG TCCCAGACTC CTCCTTGGTG AATTATGCAA ACTTCTCCTG CTTGGAGGGT 
ACAGACTTTC TGAAACAATG TTCCATCTCG TGTGTCCCAC CGGCTAAGCT CCAAGGACTG 
AACCCATGGC TGACATGTCT TGAAGATGGA CTCTGGTCTC TCCCTGAAGT CTACTGCAAG 
TTGGAATGTG AAGCTCCTCC AGTTATTCCC AATGCCAATC TGCTCCTGCC ACATTTCCTG 
GAAGGCAACC ATGATGTGGG CACCATCTGC AAATATGAGT GCAAACCAGG CTACTATGTG 
AAGGAGACTT CAGGAAGTCA AGGCAAGAAC AAGTTCCTGA AGATCCAGTG CCTGGATGAT 
GGAAGCTGGG AACAAGGGAG CTGCGTCCCC GTGGTTTGTG AGCCTCCTCC TCCGGTGTTT 
GAAGGCATGT ACGAATGTAC TGATGGCTTC AAGCTGGACA GCCAGTGTGT GCTCAACTGT 
AACCAGGAAA CGGAAGGGAT TCCCATCCTC TGCACTAAAG AGGGGTTGTG GACCCAGGAA 
TTCAAGTTGT GTGAAAACCT GCAAGGAGAA TGCCCACCAC CCACCTCAGA GCTGAATTCT 
GTGGAGTACA AGTGTGGACA GGGATATGGG ATCGGTGCAG TGTGCTCCCC ATCATGTGTA 
ATCCCCCCTA GTGACCCTGT GATACTACCA GAGAATGTGA CTACTGACAC TTTGGAGCAC 
TGGATGGAAC CTGTCAAAGT CCAGAGTATC GTGTGTACCG GCAGGCGCCA GTGGCACCCA 
GATCCCTCTC TGGTGCACTG CATCCAGTCA TGTGAGCCTT TCCAAGCAGA TGGTTGGTGT 
GACACTATTA ATAACCGGGC CTACTGCAAC TATGACGGGG GAGACTGCTG CTCTTCCACA 
CTCTCTTCCA AGAAGGTCAT TCCATTTGCT GCTGATTGTG ACTTGGATGA ATGCACCTGT 
CGAGACCCCA AGGCAGAAGA AAACCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pappa2 ( XM_006496823.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496886.1 Mus musculus pappalysin 2 (Pappa2), transcript variant X1, mRNA.

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CloneID	OMu02923
Clone ID Related Accession (Same CDS sequence)	NM_001085376.3 , XM_006496824.4 , XM_011238806.2 , XM_006496823.3 , XM_006496824.3 , NM_001085376.2
Accession Version	XM_006496823.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5370bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	pappalysin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006496823.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGTT GGAAGGTCCT GAGAATAAGC CTGGTGGTCC TGACTGGGTG GGCACTCAGT 
ACCACCAACT CAGAGCAGGG CTGGACACGC AAGAAATTCC TGGCCCAGAA GGAACACCTG 
AACCAAGTGC TGTTGGAAGG GGAACGCTGT TGGCTGGGGT ATAAGGTTCG AAGACCCAGG 
ACTGCTCCAC AGCACCATCT CTTTGGGGTC TACCCAAGCC GCCTCGAAAA CTACCCAAGG 
TCCTATCCTG TGGGGACACA GCAGCAAACC CTGTATGCAG AATGGAGTCA GCGAAGCCCA 
GAGAGACAGG CTCCGAGTGG TATTCCCCGA GACCTGACTG AAAACCCAGC AGGAGCAAGG 
AGAGAGTCTC AGCAGCCAGC TGCCCCGTGG GTAGGGGATG GTCCTATTGG GCAATCGCAG 
CTGCTGAGAA ATGATGACAC TTATCTTGGC GAGGGAGGAT CTAAGGAGGC TCTAGGTGAG 
CCTGCGATTC AGGGAAGCTC AGAAATTGCT GTCTCCACCA TTGCTACCTT CGCATATCAG 
AAGGGACCCA CAGCAGAGAC CCAGAGGAAG GGCCAGTCCA AGTCTAGGCT TCCTCGCCAA 
GTGCAGAAGA GGCAGGCAGA AGACATGACT GGAGACCCCC AGAATACCCC TCAAGGTTTC 
CAGCTTTGGC CTAAAGAGCC ACTTAAGCAT GGAGATAGTG ACAGTCCATT GGAGGGCACC 
ATCCAAAATG GTGGAGGGGC CTCTCTCCGG AGAGTAGAGA CCTTTAACTC CCAAGGAGGA 
TTGCCTGTAC TGTATTTCAC TGGGAAGAGG GAGCGGCTGT TGCTGCGTCC AGAAGTGCTG 
GCTGAGATTC CCCGGGAGGC GTTCACAGTG GAAGCCTGGG TGAGACCAGA GGGAGGACAG 
AGCAACCCAG CCATCATTGC AGGTGTGTTT GATAACTGCT CCCACACAGT CAATGACAAG 
GGCTGGGCCC TGGGGATCCG CTCAGGGAAG GACAAGGGGT GGAGAGATGC TCGCTTCTTC 
TTTTCCCTCC GCACTGACCG GATGAAGAAA GCCACTATTG TGACTGGCCA CAGCCGTTAC 
CAGCCAGGCA TGTGGACTCA TGTAGCAGCC ACTTACGATG GACAGCATAC AGCTCTGTAT 
GTGGATGGCA CTCGAGTGGC TAGTAGTCGT GACCAGTTTG GACCGCTAAA TAGCCCTTTC 
ATGGCGTCTT GCCGCTCTTT AATCCTGGGA GGGGACAGCT CCGAGGATGG ACATTATTTC 
CGTGGTTACC TGGGCACACT GGTAATCTGG TCGACCGCCC TTTCACAAAC CCACCTCCAG 
CACAGTCCCC TGCATCCAAG TGCAGCAGAT GAGTTGACCA CCCTGATCCT GACAGCCACT 
TTCGATCCTC TGATGGAACA GTGGGCTCCA TTTAGAGATG ACTTATATCC CCGGCTGGAG 
GTTCTCCAGG ACTCTGAGTC ACAGCCTGAG ATTCTGTCAC CTTTACAACC CCCACCTTGT 
GGGCAAACAG CTTGTGACAA TGTGGAACTC ATCTCCCAGT ACAATAAGCA TGGGCCCCTT 
CGTAGAGAGA AAGTGATCCG CTACCAGGTG GTAAACATCT ATGATGATGA GGGTCTGCAT 
CCCATTGTGA GTGATCATCA GATCCGCCGT CAGCATGAGG CCCTCAACCA GGCCTTCAGC 
CGCTACAATA TCAGCTGGCA GCTGAGTGTC CACCGAGTCC ACAACTCCAC CCTGCGCCAC 
CGGGTTGTGC TTGTTAATTG TGAGCCCAGC AAGATTGGCA ATGACCACTG TGACCCTGAA 
TGTGAGCATC CACTCACCGG CTTCGATGGG GGCGATTGCC GCCTGCAGGG ACGCTGCTAC 
TCCTGGAATC GCAGGGATGG CCTCTGTCAC GTGGAGTGCA ACAACATGCT GAATGACTTC 
GATGATGGAG ACTGCTGCGA TCCGGAAGTG ACTGATGTCC GAAAGACTTG CTTTGATCCT 
GACTCACCCA AGAGGGCATA CATGAGTGTG AAGGAACTAA AGGAGGCCTT GCAGCTCAAC 
AGCACTCACT TCCTTAATGT CTACTTCGCC AGCTCAGTCC GGGAAGACCT TGCAGGTTCT 
GCCACATGGC CCTGGGACAA GGAAGCTCTC AGTCACCTGG GTGGTGTTGT CCTCAACCCA 
ACCTATTATG GCATGCTTGG CCACACCAAC ATCATGATCC ATGAGGTGGG ACATGTCCTG 
GGACTCTACC ATGTATTCAA AGGAGTCAGT GAAAGAGAAT CCTGTGATGA TCCCTGTAGA 
GAGACAGTGC CATCCATGGA GACAGGAGAC CTGTGTGCTG ACACTGCTCC CACACCCAAG 
AGCAAGCTGT GTCGGGACCC AGAGCCAGCT AATGACACCT GTGGCTTCAC CCTCTTCCCA 
GGGGCTCCGT TCAATAACTA CATGAGCTAC ACAGATGATG AGTGCACTGA CAACTTCACC 
CCTAACCAAG TGGCCCGGAT GCATTGCTAC TTGGACCTCG TATACCAGCA ATGGAGTGAA 
AGCCAAAAAC CTACCCCCAT ACCCATACCA CCCATGGTTA TTGGGCAAAC CCACAAGTCC 
CTTACTATCC ATTGGCTGCC TCCAATCAGT GGAGTTGTGT ATGATAGGGC CCCTGACAGC 
ATGTGTAGTG CCTGCACTGA AGATGGGACA TTCCGTCAGT ATGTATACAA GGCATCCTCT 
GGGCGGGTGT GTGACTCTTC AGGTTACTGG ACTCCAGAGG AGGCTGTGGG GCCTCCTGAT 
GTCGATCAAC CCTGTGAGCC TAGCTTGCAG GCCTGGAGTC CTGAGCTCCA CCTAAACCAC 
ATGAATATGA CCGTGCCCTG CCCAGCAGAA GGCTGTAGCT TGGAGTTGCT TTTTCAATAC 
CCGGTCCAGG CGGATACCCT CACCCTCTGG GTCACTTACC TCTCCATGAA CTCTTCCAAG 
GCATTCTTTG ACATAGAGAT CTTGCTAGAA CACAAAAAAT CTATGCACCT TGGACCTTTA 
AACACTTTTT GTGACATACC GCTCACCATC AAACTCCACA TGGATGAGAA GGTCATGGGA 
GCGAAAGTCT ACACCTTTGA TGAACGAATG GAAATTGATG CTGCTCTCCT GACCTCTCGG 
CCCAATAGTT CCTTGTGTTC TGGATGCAAG CCTATGAGTT ACCAGGTTCT TCGAGAGCCT 
CCTTTTTCCA GTGGCTTGCC CATGGTGGTG ACACATCCAC ACAGAAAGTT CACAGATACG 
GAGGTCATCC CTGGGCAGAT GTATCAGTAC CAAGTTCAAG CTGAAGCTGT AGGGGAACTC 
GGAGAAGCTT CACCTCCTCT GAGCCACATT CACGGAGGCC CTTACTGTGG AGATGGGAAG 
GTAGAAAGGA GTATCGGAGA GATGTGTGAT GATGGAGACC TGTTGAATGG AGATGGGTGC 
TCAAGAGCAT GTGAACTGGA GGAAGGCTTC AATTGTGCAG GGGAGCCAAG CCTTTGCTAC 
AGGTATGAGG GAGATGGGGT TTGTGAAGAT TTTGAGAAGG AAAGCATCAT CACAGACTGT 
GGCCTCCACA CTCCTGAAGG ATATTTGGAT CAGTGGGCTA GCCAGGCTTA TTCCTACCAT 
GAAGACAAGG AGAAATGTCC TGTTTCCCTG GTAACAGGAG AACCTCATTC AATGATCTGT 
ACTTCATGGC ACCCGGATTC ATCCCCATAT CATTCCTTTC CTGGCTGGTT TCCCTGTGTC 
TTCAGTCTGA AGAAAGACCA GGATGCAGGC AGTGAGCAGG CTAAAGATAG CCTGCAGAAG 
GACAATGAAA TTTGGCTTGA AGTGTGTTTC AATAGGCCAG GGGTTGCAGT GGCAATTTAC 
ATTTTCTTGG CATCGGATGG CGTGACCCCT GGGGAGCATC AGCAGGCAAC AGTGACCCTT 
TACCTGATTG ATGTCAGTGG AAGCAACCAC TCTCTTGGAA CCTATGGACT GTCGTGTCAA 
CAAAATCCAC TGGTTATCAA TGTGAGCCAT CATGTGAATG TCTACCCCCA ACATACCTCC 
TCCATGCTGC TGAATTTTTC ATCCCCACTG GTGGGCATCT CAGCAGTGGC TCTAAGGACA 
TCCTCCCAGA CCAGTTCTTC AGCTCCCAGT AACTGCATCC CAGAGCAAAA TTATGAGGGA 
AAGAGCTGTG CTCAACGACC TTGTGGAGAA CAGGGAAGAT GTGCGCCATT GCTACTCGAT 
CATACGGACA TGGTGAATTG TACCTCCAGT AGCCCAGGTC ACATGGAGTG CGCTATCACT 
TGTCAAAGGG GGTATGTCCT TCAGACCAGC AGTGGGCAGT ACCTGAGGAA CATGCAGAAA 
GAGATTCTCC TTACTTGTTC CTCTGGCCAC TGGGACAAGG ATGTGATCTG CAAGCGTCTT 
GACTGTGGTG TCCCAGACTC CTCCTTGGTG AATTATGCAA ACTTCTCCTG CTTGGAGGGT 
ACAGACTTTC TGAAACAATG TTCCATCTCG TGTGTCCCAC CGGCTAAGCT CCAAGGACTG 
AACCCATGGC TGACATGTCT TGAAGATGGA CTCTGGTCTC TCCCTGAAGT CTACTGCAAG 
TTGGAATGTG AAGCTCCTCC AGTTATTCCC AATGCCAATC TGCTCCTGCC ACATTTCCTG 
GAAGGCAACC ATGATGTGGG CACCATCTGC AAATATGAGT GCAAACCAGG CTACTATGTG 
AAGGAGACTT CAGGAAGTCA AGGCAAGAAC AAGTTCCTGA AGATCCAGTG CCTGGATGAT 
GGAAGCTGGG AACAAGGGAG CTGCGTCCCC GTGGTTTGTG AGCCTCCTCC TCCGGTGTTT 
GAAGGCATGT ACGAATGTAC TGATGGCTTC AAGCTGGACA GCCAGTGTGT GCTCAACTGT 
AACCAGGAAA CGGAAGGGAT TCCCATCCTC TGCACTAAAG AGGGGTTGTG GACCCAGGAA 
TTCAAGTTGT GTGAAAACCT GCAAGGAGAA TGCCCACCAC CCACCTCAGA GCTGAATTCT 
GTGGAGTACA AGTGTGGACA GGGATATGGG ATCGGTGCAG TGTGCTCCCC ATCATGTGTA 
ATCCCCCCTA GTGACCCTGT GATACTACCA GAGAATGTGA CTACTGACAC TTTGGAGCAC 
TGGATGGAAC CTGTCAAAGT CCAGAGTATC GTGTGTACCG GCAGGCGCCA GTGGCACCCA 
GATCCCTCTC TGGTGCACTG CATCCAGTCA TGTGAGCCTT TCCAAGCAGA TGGTTGGTGT 
GACACTATTA ATAACCGGGC CTACTGCAAC TATGACGGGG GAGACTGCTG CTCTTCCACA 
CTCTCTTCCA AGAAGGTCAT TCCATTTGCT GCTGATTGTG ACTTGGATGA ATGCACCTGT 
CGAGACCCCA AGGCAGAAGA AAACCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496886.1
CDS	3268..8637
Translation	MMCWKVLRISLVVLTGWALSTTNSEQGWTRKKFLAQKEHLNQVLLEGERCWLGYKVRRPRTAPQHHLFGVYPSRLENYPRSYPVGTQQQTLYAEWSQRSPERQAPSGIPRDLTENPAGARRESQQPAAPWVGDGPIGQSQLLRNDDTYLGEGGSKEALGEPAIQGSSEIAVSTIATFAYQKGPTAETQRKGQSKSRLPRQVQKRQAEDMTGDPQNTPQGFQLWPKEPLKHGDSDSPLEGTIQNGGGASLRRVETFNSQGGLPVLYFTGKRERLLLRPEVLAEIPREAFTVEAWVRPEGGQSNPAIIAGVFDNCSHTVNDKGWALGIRSGKDKGWRDARFFFSLRTDRMKKATIVTGHSRYQPGMWTHVAATYDGQHTALYVDGTRVASSRDQFGPLNSPFMASCRSLILGGDSSEDGHYFRGYLGTLVIWSTALSQTHLQHSPLHPSAADELTTLILTATFDPLMEQWAPFRDDLYPRLEVLQDSESQPEILSPLQPPPCGQTACDNVELISQYNKHGPLRREKVIRYQVVNIYDDEGLHPIVSDHQIRRQHEALNQAFSRYNISWQLSVHRVHNSTLRHRVVLVNCEPSKIGNDHCDPECEHPLTGFDGGDCRLQGRCYSWNRRDGLCHVECNNMLNDFDDGDCCDPEVTDVRKTCFDPDSPKRAYMSVKELKEALQLNSTHFLNVYFASSVREDLAGSATWPWDKEALSHLGGVVLNPTYYGMLGHTNIMIHEVGHVLGLYHVFKGVSERESCDDPCRETVPSMETGDLCADTAPTPKSKLCRDPEPANDTCGFTLFPGAPFNNYMSYTDDECTDNFTPNQVARMHCYLDLVYQQWSESQKPTPIPIPPMVIGQTHKSLTIHWLPPISGVVYDRAPDSMCSACTEDGTFRQYVYKASSGRVCDSSGYWTPEEAVGPPDVDQPCEPSLQAWSPELHLNHMNMTVPCPAEGCSLELLFQYPVQADTLTLWVTYLSMNSSKAFFDIEILLEHKKSMHLGPLNTFCDIPLTIKLHMDEKVMGAKVYTFDERMEIDAALLTSRPNSSLCSGCKPMSYQVLREPPFSSGLPMVVTHPHRKFTDTEVIPGQMYQYQVQAEAVGELGEASPPLSHIHGGPYCGDGKVERSIGEMCDDGDLLNGDGCSRACELEEGFNCAGEPSLCYRYEGDGVCEDFEKESIITDCGLHTPEGYLDQWASQAYSYHEDKEKCPVSLVTGEPHSMICTSWHPDSSPYHSFPGWFPCVFSLKKDQDAGSEQAKDSLQKDNEIWLEVCFNRPGVAVAIYIFLASDGVTPGEHQQATVTLYLIDVSGSNHSLGTYGLSCQQNPLVINVSHHVNVYPQHTSSMLLNFSSPLVGISAVALRTSSQTSSSAPSNCIPEQNYEGKSCAQRPCGEQGRCAPLLLDHTDMVNCTSSSPGHMECAITCQRGYVLQTSSGQYLRNMQKEILLTCSSGHWDKDVICKRLDCGVPDSSLVNYANFSCLEGTDFLKQCSISCVPPAKLQGLNPWLTCLEDGLWSLPEVYCKLECEAPPVIPNANLLLPHFLEGNHDVGTICKYECKPGYYVKETSGSQGKNKFLKIQCLDDGSWEQGSCVPVVCEPPPPVFEGMYECTDGFKLDSQCVLNCNQETEGIPILCTKEGLWTQEFKLCENLQGECPPPTSELNSVEYKCGQGYGIGAVCSPSCVIPPSDPVILPENVTTDTLEHWMEPVKVQSIVCTGRRQWHPDPSLVHCIQSCEPFQADGWCDTINNRAYCNYDGGDCCSSTLSSKKVIPFAADCDLDECTCRDPKAEENQ

Target ORF information:

RefSeq Version	XM_006496823.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus pappalysin 2 (Pappa2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496823.3

ORF Insert Sequence:

ATGATGTGTT GGAAGGTCCT GAGAATAAGC CTGGTGGTCC TGACTGGGTG GGCACTCAGT 
ACCACCAACT CAGAGCAGGG CTGGACACGC AAGAAATTCC TGGCCCAGAA GGAACACCTG 
AACCAAGTGC TGTTGGAAGG GGAACGCTGT TGGCTGGGGT ATAAGGTTCG AAGACCCAGG 
ACTGCTCCAC AGCACCATCT CTTTGGGGTC TACCCAAGCC GCCTCGAAAA CTACCCAAGG 
TCCTATCCTG TGGGGACACA GCAGCAAACC CTGTATGCAG AATGGAGTCA GCGAAGCCCA 
GAGAGACAGG CTCCGAGTGG TATTCCCCGA GACCTGACTG AAAACCCAGC AGGAGCAAGG 
AGAGAGTCTC AGCAGCCAGC TGCCCCGTGG GTAGGGGATG GTCCTATTGG GCAATCGCAG 
CTGCTGAGAA ATGATGACAC TTATCTTGGC GAGGGAGGAT CTAAGGAGGC TCTAGGTGAG 
CCTGCGATTC AGGGAAGCTC AGAAATTGCT GTCTCCACCA TTGCTACCTT CGCATATCAG 
AAGGGACCCA CAGCAGAGAC CCAGAGGAAG GGCCAGTCCA AGTCTAGGCT TCCTCGCCAA 
GTGCAGAAGA GGCAGGCAGA AGACATGACT GGAGACCCCC AGAATACCCC TCAAGGTTTC 
CAGCTTTGGC CTAAAGAGCC ACTTAAGCAT GGAGATAGTG ACAGTCCATT GGAGGGCACC 
ATCCAAAATG GTGGAGGGGC CTCTCTCCGG AGAGTAGAGA CCTTTAACTC CCAAGGAGGA 
TTGCCTGTAC TGTATTTCAC TGGGAAGAGG GAGCGGCTGT TGCTGCGTCC AGAAGTGCTG 
GCTGAGATTC CCCGGGAGGC GTTCACAGTG GAAGCCTGGG TGAGACCAGA GGGAGGACAG 
AGCAACCCAG CCATCATTGC AGGTGTGTTT GATAACTGCT CCCACACAGT CAATGACAAG 
GGCTGGGCCC TGGGGATCCG CTCAGGGAAG GACAAGGGGT GGAGAGATGC TCGCTTCTTC 
TTTTCCCTCC GCACTGACCG GATGAAGAAA GCCACTATTG TGACTGGCCA CAGCCGTTAC 
CAGCCAGGCA TGTGGACTCA TGTAGCAGCC ACTTACGATG GACAGCATAC AGCTCTGTAT 
GTGGATGGCA CTCGAGTGGC TAGTAGTCGT GACCAGTTTG GACCGCTAAA TAGCCCTTTC 
ATGGCGTCTT GCCGCTCTTT AATCCTGGGA GGGGACAGCT CCGAGGATGG ACATTATTTC 
CGTGGTTACC TGGGCACACT GGTAATCTGG TCGACCGCCC TTTCACAAAC CCACCTCCAG 
CACAGTCCCC TGCATCCAAG TGCAGCAGAT GAGTTGACCA CCCTGATCCT GACAGCCACT 
TTCGATCCTC TGATGGAACA GTGGGCTCCA TTTAGAGATG ACTTATATCC CCGGCTGGAG 
GTTCTCCAGG ACTCTGAGTC ACAGCCTGAG ATTCTGTCAC CTTTACAACC CCCACCTTGT 
GGGCAAACAG CTTGTGACAA TGTGGAACTC ATCTCCCAGT ACAATAAGCA TGGGCCCCTT 
CGTAGAGAGA AAGTGATCCG CTACCAGGTG GTAAACATCT ATGATGATGA GGGTCTGCAT 
CCCATTGTGA GTGATCATCA GATCCGCCGT CAGCATGAGG CCCTCAACCA GGCCTTCAGC 
CGCTACAATA TCAGCTGGCA GCTGAGTGTC CACCGAGTCC ACAACTCCAC CCTGCGCCAC 
CGGGTTGTGC TTGTTAATTG TGAGCCCAGC AAGATTGGCA ATGACCACTG TGACCCTGAA 
TGTGAGCATC CACTCACCGG CTTCGATGGG GGCGATTGCC GCCTGCAGGG ACGCTGCTAC 
TCCTGGAATC GCAGGGATGG CCTCTGTCAC GTGGAGTGCA ACAACATGCT GAATGACTTC 
GATGATGGAG ACTGCTGCGA TCCGGAAGTG ACTGATGTCC GAAAGACTTG CTTTGATCCT 
GACTCACCCA AGAGGGCATA CATGAGTGTG AAGGAACTAA AGGAGGCCTT GCAGCTCAAC 
AGCACTCACT TCCTTAATGT CTACTTCGCC AGCTCAGTCC GGGAAGACCT TGCAGGTTCT 
GCCACATGGC CCTGGGACAA GGAAGCTCTC AGTCACCTGG GTGGTGTTGT CCTCAACCCA 
ACCTATTATG GCATGCTTGG CCACACCAAC ATCATGATCC ATGAGGTGGG ACATGTCCTG 
GGACTCTACC ATGTATTCAA AGGAGTCAGT GAAAGAGAAT CCTGTGATGA TCCCTGTAGA 
GAGACAGTGC CATCCATGGA GACAGGAGAC CTGTGTGCTG ACACTGCTCC CACACCCAAG 
AGCAAGCTGT GTCGGGACCC AGAGCCAGCT AATGACACCT GTGGCTTCAC CCTCTTCCCA 
GGGGCTCCGT TCAATAACTA CATGAGCTAC ACAGATGATG AGTGCACTGA CAACTTCACC 
CCTAACCAAG TGGCCCGGAT GCATTGCTAC TTGGACCTCG TATACCAGCA ATGGAGTGAA 
AGCCAAAAAC CTACCCCCAT ACCCATACCA CCCATGGTTA TTGGGCAAAC CCACAAGTCC 
CTTACTATCC ATTGGCTGCC TCCAATCAGT GGAGTTGTGT ATGATAGGGC CCCTGACAGC 
ATGTGTAGTG CCTGCACTGA AGATGGGACA TTCCGTCAGT ATGTATACAA GGCATCCTCT 
GGGCGGGTGT GTGACTCTTC AGGTTACTGG ACTCCAGAGG AGGCTGTGGG GCCTCCTGAT 
GTCGATCAAC CCTGTGAGCC TAGCTTGCAG GCCTGGAGTC CTGAGCTCCA CCTAAACCAC 
ATGAATATGA CCGTGCCCTG CCCAGCAGAA GGCTGTAGCT TGGAGTTGCT TTTTCAATAC 
CCGGTCCAGG CGGATACCCT CACCCTCTGG GTCACTTACC TCTCCATGAA CTCTTCCAAG 
GCATTCTTTG ACATAGAGAT CTTGCTAGAA CACAAAAAAT CTATGCACCT TGGACCTTTA 
AACACTTTTT GTGACATACC GCTCACCATC AAACTCCACA TGGATGAGAA GGTCATGGGA 
GCGAAAGTCT ACACCTTTGA TGAACGAATG GAAATTGATG CTGCTCTCCT GACCTCTCGG 
CCCAATAGTT CCTTGTGTTC TGGATGCAAG CCTATGAGTT ACCAGGTTCT TCGAGAGCCT 
CCTTTTTCCA GTGGCTTGCC CATGGTGGTG ACACATCCAC ACAGAAAGTT CACAGATACG 
GAGGTCATCC CTGGGCAGAT GTATCAGTAC CAAGTTCAAG CTGAAGCTGT AGGGGAACTC 
GGAGAAGCTT CACCTCCTCT GAGCCACATT CACGGAGGCC CTTACTGTGG AGATGGGAAG 
GTAGAAAGGA GTATCGGAGA GATGTGTGAT GATGGAGACC TGTTGAATGG AGATGGGTGC 
TCAAGAGCAT GTGAACTGGA GGAAGGCTTC AATTGTGCAG GGGAGCCAAG CCTTTGCTAC 
AGGTATGAGG GAGATGGGGT TTGTGAAGAT TTTGAGAAGG AAAGCATCAT CACAGACTGT 
GGCCTCCACA CTCCTGAAGG ATATTTGGAT CAGTGGGCTA GCCAGGCTTA TTCCTACCAT 
GAAGACAAGG AGAAATGTCC TGTTTCCCTG GTAACAGGAG AACCTCATTC AATGATCTGT 
ACTTCATGGC ACCCGGATTC ATCCCCATAT CATTCCTTTC CTGGCTGGTT TCCCTGTGTC 
TTCAGTCTGA AGAAAGACCA GGATGCAGGC AGTGAGCAGG CTAAAGATAG CCTGCAGAAG 
GACAATGAAA TTTGGCTTGA AGTGTGTTTC AATAGGCCAG GGGTTGCAGT GGCAATTTAC 
ATTTTCTTGG CATCGGATGG CGTGACCCCT GGGGAGCATC AGCAGGCAAC AGTGACCCTT 
TACCTGATTG ATGTCAGTGG AAGCAACCAC TCTCTTGGAA CCTATGGACT GTCGTGTCAA 
CAAAATCCAC TGGTTATCAA TGTGAGCCAT CATGTGAATG TCTACCCCCA ACATACCTCC 
TCCATGCTGC TGAATTTTTC ATCCCCACTG GTGGGCATCT CAGCAGTGGC TCTAAGGACA 
TCCTCCCAGA CCAGTTCTTC AGCTCCCAGT AACTGCATCC CAGAGCAAAA TTATGAGGGA 
AAGAGCTGTG CTCAACGACC TTGTGGAGAA CAGGGAAGAT GTGCGCCATT GCTACTCGAT 
CATACGGACA TGGTGAATTG TACCTCCAGT AGCCCAGGTC ACATGGAGTG CGCTATCACT 
TGTCAAAGGG GGTATGTCCT TCAGACCAGC AGTGGGCAGT ACCTGAGGAA CATGCAGAAA 
GAGATTCTCC TTACTTGTTC CTCTGGCCAC TGGGACAAGG ATGTGATCTG CAAGCGTCTT 
GACTGTGGTG TCCCAGACTC CTCCTTGGTG AATTATGCAA ACTTCTCCTG CTTGGAGGGT 
ACAGACTTTC TGAAACAATG TTCCATCTCG TGTGTCCCAC CGGCTAAGCT CCAAGGACTG 
AACCCATGGC TGACATGTCT TGAAGATGGA CTCTGGTCTC TCCCTGAAGT CTACTGCAAG 
TTGGAATGTG AAGCTCCTCC AGTTATTCCC AATGCCAATC TGCTCCTGCC ACATTTCCTG 
GAAGGCAACC ATGATGTGGG CACCATCTGC AAATATGAGT GCAAACCAGG CTACTATGTG 
AAGGAGACTT CAGGAAGTCA AGGCAAGAAC AAGTTCCTGA AGATCCAGTG CCTGGATGAT 
GGAAGCTGGG AACAAGGGAG CTGCGTCCCC GTGGTTTGTG AGCCTCCTCC TCCGGTGTTT 
GAAGGCATGT ACGAATGTAC TGATGGCTTC AAGCTGGACA GCCAGTGTGT GCTCAACTGT 
AACCAGGAAA CGGAAGGGAT TCCCATCCTC TGCACTAAAG AGGGGTTGTG GACCCAGGAA 
TTCAAGTTGT GTGAAAACCT GCAAGGAGAA TGCCCACCAC CCACCTCAGA GCTGAATTCT 
GTGGAGTACA AGTGTGGACA GGGATATGGG ATCGGTGCAG TGTGCTCCCC ATCATGTGTA 
ATCCCCCCTA GTGACCCTGT GATACTACCA GAGAATGTGA CTACTGACAC TTTGGAGCAC 
TGGATGGAAC CTGTCAAAGT CCAGAGTATC GTGTGTACCG GCAGGCGCCA GTGGCACCCA 
GATCCCTCTC TGGTGCACTG CATCCAGTCA TGTGAGCCTT TCCAAGCAGA TGGTTGGTGT 
GACACTATTA ATAACCGGGC CTACTGCAAC TATGACGGGG GAGACTGCTG CTCTTCCACA 
CTCTCTTCCA AGAAGGTCAT TCCATTTGCT GCTGATTGTG ACTTGGATGA ATGCACCTGT 
CGAGACCCCA AGGCAGAAGA AAACCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pappa2 ( XM_006496824.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496887.1 Mus musculus pappalysin 2 (Pappa2), transcript variant X3, mRNA.

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CloneID	OMu02923
Clone ID Related Accession (Same CDS sequence)	NM_001085376.3 , XM_006496824.4 , XM_011238806.2 , XM_006496823.3 , XM_006496824.3 , NM_001085376.2
Accession Version	XM_006496824.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5370bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	pappalysin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006496824.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGTT GGAAGGTCCT GAGAATAAGC CTGGTGGTCC TGACTGGGTG GGCACTCAGT 
ACCACCAACT CAGAGCAGGG CTGGACACGC AAGAAATTCC TGGCCCAGAA GGAACACCTG 
AACCAAGTGC TGTTGGAAGG GGAACGCTGT TGGCTGGGGT ATAAGGTTCG AAGACCCAGG 
ACTGCTCCAC AGCACCATCT CTTTGGGGTC TACCCAAGCC GCCTCGAAAA CTACCCAAGG 
TCCTATCCTG TGGGGACACA GCAGCAAACC CTGTATGCAG AATGGAGTCA GCGAAGCCCA 
GAGAGACAGG CTCCGAGTGG TATTCCCCGA GACCTGACTG AAAACCCAGC AGGAGCAAGG 
AGAGAGTCTC AGCAGCCAGC TGCCCCGTGG GTAGGGGATG GTCCTATTGG GCAATCGCAG 
CTGCTGAGAA ATGATGACAC TTATCTTGGC GAGGGAGGAT CTAAGGAGGC TCTAGGTGAG 
CCTGCGATTC AGGGAAGCTC AGAAATTGCT GTCTCCACCA TTGCTACCTT CGCATATCAG 
AAGGGACCCA CAGCAGAGAC CCAGAGGAAG GGCCAGTCCA AGTCTAGGCT TCCTCGCCAA 
GTGCAGAAGA GGCAGGCAGA AGACATGACT GGAGACCCCC AGAATACCCC TCAAGGTTTC 
CAGCTTTGGC CTAAAGAGCC ACTTAAGCAT GGAGATAGTG ACAGTCCATT GGAGGGCACC 
ATCCAAAATG GTGGAGGGGC CTCTCTCCGG AGAGTAGAGA CCTTTAACTC CCAAGGAGGA 
TTGCCTGTAC TGTATTTCAC TGGGAAGAGG GAGCGGCTGT TGCTGCGTCC AGAAGTGCTG 
GCTGAGATTC CCCGGGAGGC GTTCACAGTG GAAGCCTGGG TGAGACCAGA GGGAGGACAG 
AGCAACCCAG CCATCATTGC AGGTGTGTTT GATAACTGCT CCCACACAGT CAATGACAAG 
GGCTGGGCCC TGGGGATCCG CTCAGGGAAG GACAAGGGGT GGAGAGATGC TCGCTTCTTC 
TTTTCCCTCC GCACTGACCG GATGAAGAAA GCCACTATTG TGACTGGCCA CAGCCGTTAC 
CAGCCAGGCA TGTGGACTCA TGTAGCAGCC ACTTACGATG GACAGCATAC AGCTCTGTAT 
GTGGATGGCA CTCGAGTGGC TAGTAGTCGT GACCAGTTTG GACCGCTAAA TAGCCCTTTC 
ATGGCGTCTT GCCGCTCTTT AATCCTGGGA GGGGACAGCT CCGAGGATGG ACATTATTTC 
CGTGGTTACC TGGGCACACT GGTAATCTGG TCGACCGCCC TTTCACAAAC CCACCTCCAG 
CACAGTCCCC TGCATCCAAG TGCAGCAGAT GAGTTGACCA CCCTGATCCT GACAGCCACT 
TTCGATCCTC TGATGGAACA GTGGGCTCCA TTTAGAGATG ACTTATATCC CCGGCTGGAG 
GTTCTCCAGG ACTCTGAGTC ACAGCCTGAG ATTCTGTCAC CTTTACAACC CCCACCTTGT 
GGGCAAACAG CTTGTGACAA TGTGGAACTC ATCTCCCAGT ACAATAAGCA TGGGCCCCTT 
CGTAGAGAGA AAGTGATCCG CTACCAGGTG GTAAACATCT ATGATGATGA GGGTCTGCAT 
CCCATTGTGA GTGATCATCA GATCCGCCGT CAGCATGAGG CCCTCAACCA GGCCTTCAGC 
CGCTACAATA TCAGCTGGCA GCTGAGTGTC CACCGAGTCC ACAACTCCAC CCTGCGCCAC 
CGGGTTGTGC TTGTTAATTG TGAGCCCAGC AAGATTGGCA ATGACCACTG TGACCCTGAA 
TGTGAGCATC CACTCACCGG CTTCGATGGG GGCGATTGCC GCCTGCAGGG ACGCTGCTAC 
TCCTGGAATC GCAGGGATGG CCTCTGTCAC GTGGAGTGCA ACAACATGCT GAATGACTTC 
GATGATGGAG ACTGCTGCGA TCCGGAAGTG ACTGATGTCC GAAAGACTTG CTTTGATCCT 
GACTCACCCA AGAGGGCATA CATGAGTGTG AAGGAACTAA AGGAGGCCTT GCAGCTCAAC 
AGCACTCACT TCCTTAATGT CTACTTCGCC AGCTCAGTCC GGGAAGACCT TGCAGGTTCT 
GCCACATGGC CCTGGGACAA GGAAGCTCTC AGTCACCTGG GTGGTGTTGT CCTCAACCCA 
ACCTATTATG GCATGCTTGG CCACACCAAC ATCATGATCC ATGAGGTGGG ACATGTCCTG 
GGACTCTACC ATGTATTCAA AGGAGTCAGT GAAAGAGAAT CCTGTGATGA TCCCTGTAGA 
GAGACAGTGC CATCCATGGA GACAGGAGAC CTGTGTGCTG ACACTGCTCC CACACCCAAG 
AGCAAGCTGT GTCGGGACCC AGAGCCAGCT AATGACACCT GTGGCTTCAC CCTCTTCCCA 
GGGGCTCCGT TCAATAACTA CATGAGCTAC ACAGATGATG AGTGCACTGA CAACTTCACC 
CCTAACCAAG TGGCCCGGAT GCATTGCTAC TTGGACCTCG TATACCAGCA ATGGAGTGAA 
AGCCAAAAAC CTACCCCCAT ACCCATACCA CCCATGGTTA TTGGGCAAAC CCACAAGTCC 
CTTACTATCC ATTGGCTGCC TCCAATCAGT GGAGTTGTGT ATGATAGGGC CCCTGACAGC 
ATGTGTAGTG CCTGCACTGA AGATGGGACA TTCCGTCAGT ATGTATACAA GGCATCCTCT 
GGGCGGGTGT GTGACTCTTC AGGTTACTGG ACTCCAGAGG AGGCTGTGGG GCCTCCTGAT 
GTCGATCAAC CCTGTGAGCC TAGCTTGCAG GCCTGGAGTC CTGAGCTCCA CCTAAACCAC 
ATGAATATGA CCGTGCCCTG CCCAGCAGAA GGCTGTAGCT TGGAGTTGCT TTTTCAATAC 
CCGGTCCAGG CGGATACCCT CACCCTCTGG GTCACTTACC TCTCCATGAA CTCTTCCAAG 
GCATTCTTTG ACATAGAGAT CTTGCTAGAA CACAAAAAAT CTATGCACCT TGGACCTTTA 
AACACTTTTT GTGACATACC GCTCACCATC AAACTCCACA TGGATGAGAA GGTCATGGGA 
GCGAAAGTCT ACACCTTTGA TGAACGAATG GAAATTGATG CTGCTCTCCT GACCTCTCGG 
CCCAATAGTT CCTTGTGTTC TGGATGCAAG CCTATGAGTT ACCAGGTTCT TCGAGAGCCT 
CCTTTTTCCA GTGGCTTGCC CATGGTGGTG ACACATCCAC ACAGAAAGTT CACAGATACG 
GAGGTCATCC CTGGGCAGAT GTATCAGTAC CAAGTTCAAG CTGAAGCTGT AGGGGAACTC 
GGAGAAGCTT CACCTCCTCT GAGCCACATT CACGGAGGCC CTTACTGTGG AGATGGGAAG 
GTAGAAAGGA GTATCGGAGA GATGTGTGAT GATGGAGACC TGTTGAATGG AGATGGGTGC 
TCAAGAGCAT GTGAACTGGA GGAAGGCTTC AATTGTGCAG GGGAGCCAAG CCTTTGCTAC 
AGGTATGAGG GAGATGGGGT TTGTGAAGAT TTTGAGAAGG AAAGCATCAT CACAGACTGT 
GGCCTCCACA CTCCTGAAGG ATATTTGGAT CAGTGGGCTA GCCAGGCTTA TTCCTACCAT 
GAAGACAAGG AGAAATGTCC TGTTTCCCTG GTAACAGGAG AACCTCATTC AATGATCTGT 
ACTTCATGGC ACCCGGATTC ATCCCCATAT CATTCCTTTC CTGGCTGGTT TCCCTGTGTC 
TTCAGTCTGA AGAAAGACCA GGATGCAGGC AGTGAGCAGG CTAAAGATAG CCTGCAGAAG 
GACAATGAAA TTTGGCTTGA AGTGTGTTTC AATAGGCCAG GGGTTGCAGT GGCAATTTAC 
ATTTTCTTGG CATCGGATGG CGTGACCCCT GGGGAGCATC AGCAGGCAAC AGTGACCCTT 
TACCTGATTG ATGTCAGTGG AAGCAACCAC TCTCTTGGAA CCTATGGACT GTCGTGTCAA 
CAAAATCCAC TGGTTATCAA TGTGAGCCAT CATGTGAATG TCTACCCCCA ACATACCTCC 
TCCATGCTGC TGAATTTTTC ATCCCCACTG GTGGGCATCT CAGCAGTGGC TCTAAGGACA 
TCCTCCCAGA CCAGTTCTTC AGCTCCCAGT AACTGCATCC CAGAGCAAAA TTATGAGGGA 
AAGAGCTGTG CTCAACGACC TTGTGGAGAA CAGGGAAGAT GTGCGCCATT GCTACTCGAT 
CATACGGACA TGGTGAATTG TACCTCCAGT AGCCCAGGTC ACATGGAGTG CGCTATCACT 
TGTCAAAGGG GGTATGTCCT TCAGACCAGC AGTGGGCAGT ACCTGAGGAA CATGCAGAAA 
GAGATTCTCC TTACTTGTTC CTCTGGCCAC TGGGACAAGG ATGTGATCTG CAAGCGTCTT 
GACTGTGGTG TCCCAGACTC CTCCTTGGTG AATTATGCAA ACTTCTCCTG CTTGGAGGGT 
ACAGACTTTC TGAAACAATG TTCCATCTCG TGTGTCCCAC CGGCTAAGCT CCAAGGACTG 
AACCCATGGC TGACATGTCT TGAAGATGGA CTCTGGTCTC TCCCTGAAGT CTACTGCAAG 
TTGGAATGTG AAGCTCCTCC AGTTATTCCC AATGCCAATC TGCTCCTGCC ACATTTCCTG 
GAAGGCAACC ATGATGTGGG CACCATCTGC AAATATGAGT GCAAACCAGG CTACTATGTG 
AAGGAGACTT CAGGAAGTCA AGGCAAGAAC AAGTTCCTGA AGATCCAGTG CCTGGATGAT 
GGAAGCTGGG AACAAGGGAG CTGCGTCCCC GTGGTTTGTG AGCCTCCTCC TCCGGTGTTT 
GAAGGCATGT ACGAATGTAC TGATGGCTTC AAGCTGGACA GCCAGTGTGT GCTCAACTGT 
AACCAGGAAA CGGAAGGGAT TCCCATCCTC TGCACTAAAG AGGGGTTGTG GACCCAGGAA 
TTCAAGTTGT GTGAAAACCT GCAAGGAGAA TGCCCACCAC CCACCTCAGA GCTGAATTCT 
GTGGAGTACA AGTGTGGACA GGGATATGGG ATCGGTGCAG TGTGCTCCCC ATCATGTGTA 
ATCCCCCCTA GTGACCCTGT GATACTACCA GAGAATGTGA CTACTGACAC TTTGGAGCAC 
TGGATGGAAC CTGTCAAAGT CCAGAGTATC GTGTGTACCG GCAGGCGCCA GTGGCACCCA 
GATCCCTCTC TGGTGCACTG CATCCAGTCA TGTGAGCCTT TCCAAGCAGA TGGTTGGTGT 
GACACTATTA ATAACCGGGC CTACTGCAAC TATGACGGGG GAGACTGCTG CTCTTCCACA 
CTCTCTTCCA AGAAGGTCAT TCCATTTGCT GCTGATTGTG ACTTGGATGA ATGCACCTGT 
CGAGACCCCA AGGCAGAAGA AAACCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496887.1
CDS	910..6279
Translation	MMCWKVLRISLVVLTGWALSTTNSEQGWTRKKFLAQKEHLNQVLLEGERCWLGYKVRRPRTAPQHHLFGVYPSRLENYPRSYPVGTQQQTLYAEWSQRSPERQAPSGIPRDLTENPAGARRESQQPAAPWVGDGPIGQSQLLRNDDTYLGEGGSKEALGEPAIQGSSEIAVSTIATFAYQKGPTAETQRKGQSKSRLPRQVQKRQAEDMTGDPQNTPQGFQLWPKEPLKHGDSDSPLEGTIQNGGGASLRRVETFNSQGGLPVLYFTGKRERLLLRPEVLAEIPREAFTVEAWVRPEGGQSNPAIIAGVFDNCSHTVNDKGWALGIRSGKDKGWRDARFFFSLRTDRMKKATIVTGHSRYQPGMWTHVAATYDGQHTALYVDGTRVASSRDQFGPLNSPFMASCRSLILGGDSSEDGHYFRGYLGTLVIWSTALSQTHLQHSPLHPSAADELTTLILTATFDPLMEQWAPFRDDLYPRLEVLQDSESQPEILSPLQPPPCGQTACDNVELISQYNKHGPLRREKVIRYQVVNIYDDEGLHPIVSDHQIRRQHEALNQAFSRYNISWQLSVHRVHNSTLRHRVVLVNCEPSKIGNDHCDPECEHPLTGFDGGDCRLQGRCYSWNRRDGLCHVECNNMLNDFDDGDCCDPEVTDVRKTCFDPDSPKRAYMSVKELKEALQLNSTHFLNVYFASSVREDLAGSATWPWDKEALSHLGGVVLNPTYYGMLGHTNIMIHEVGHVLGLYHVFKGVSERESCDDPCRETVPSMETGDLCADTAPTPKSKLCRDPEPANDTCGFTLFPGAPFNNYMSYTDDECTDNFTPNQVARMHCYLDLVYQQWSESQKPTPIPIPPMVIGQTHKSLTIHWLPPISGVVYDRAPDSMCSACTEDGTFRQYVYKASSGRVCDSSGYWTPEEAVGPPDVDQPCEPSLQAWSPELHLNHMNMTVPCPAEGCSLELLFQYPVQADTLTLWVTYLSMNSSKAFFDIEILLEHKKSMHLGPLNTFCDIPLTIKLHMDEKVMGAKVYTFDERMEIDAALLTSRPNSSLCSGCKPMSYQVLREPPFSSGLPMVVTHPHRKFTDTEVIPGQMYQYQVQAEAVGELGEASPPLSHIHGGPYCGDGKVERSIGEMCDDGDLLNGDGCSRACELEEGFNCAGEPSLCYRYEGDGVCEDFEKESIITDCGLHTPEGYLDQWASQAYSYHEDKEKCPVSLVTGEPHSMICTSWHPDSSPYHSFPGWFPCVFSLKKDQDAGSEQAKDSLQKDNEIWLEVCFNRPGVAVAIYIFLASDGVTPGEHQQATVTLYLIDVSGSNHSLGTYGLSCQQNPLVINVSHHVNVYPQHTSSMLLNFSSPLVGISAVALRTSSQTSSSAPSNCIPEQNYEGKSCAQRPCGEQGRCAPLLLDHTDMVNCTSSSPGHMECAITCQRGYVLQTSSGQYLRNMQKEILLTCSSGHWDKDVICKRLDCGVPDSSLVNYANFSCLEGTDFLKQCSISCVPPAKLQGLNPWLTCLEDGLWSLPEVYCKLECEAPPVIPNANLLLPHFLEGNHDVGTICKYECKPGYYVKETSGSQGKNKFLKIQCLDDGSWEQGSCVPVVCEPPPPVFEGMYECTDGFKLDSQCVLNCNQETEGIPILCTKEGLWTQEFKLCENLQGECPPPTSELNSVEYKCGQGYGIGAVCSPSCVIPPSDPVILPENVTTDTLEHWMEPVKVQSIVCTGRRQWHPDPSLVHCIQSCEPFQADGWCDTINNRAYCNYDGGDCCSSTLSSKKVIPFAADCDLDECTCRDPKAEENQ

Target ORF information:

RefSeq Version	XM_006496824.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus pappalysin 2 (Pappa2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496824.3

ORF Insert Sequence:

ATGATGTGTT GGAAGGTCCT GAGAATAAGC CTGGTGGTCC TGACTGGGTG GGCACTCAGT 
ACCACCAACT CAGAGCAGGG CTGGACACGC AAGAAATTCC TGGCCCAGAA GGAACACCTG 
AACCAAGTGC TGTTGGAAGG GGAACGCTGT TGGCTGGGGT ATAAGGTTCG AAGACCCAGG 
ACTGCTCCAC AGCACCATCT CTTTGGGGTC TACCCAAGCC GCCTCGAAAA CTACCCAAGG 
TCCTATCCTG TGGGGACACA GCAGCAAACC CTGTATGCAG AATGGAGTCA GCGAAGCCCA 
GAGAGACAGG CTCCGAGTGG TATTCCCCGA GACCTGACTG AAAACCCAGC AGGAGCAAGG 
AGAGAGTCTC AGCAGCCAGC TGCCCCGTGG GTAGGGGATG GTCCTATTGG GCAATCGCAG 
CTGCTGAGAA ATGATGACAC TTATCTTGGC GAGGGAGGAT CTAAGGAGGC TCTAGGTGAG 
CCTGCGATTC AGGGAAGCTC AGAAATTGCT GTCTCCACCA TTGCTACCTT CGCATATCAG 
AAGGGACCCA CAGCAGAGAC CCAGAGGAAG GGCCAGTCCA AGTCTAGGCT TCCTCGCCAA 
GTGCAGAAGA GGCAGGCAGA AGACATGACT GGAGACCCCC AGAATACCCC TCAAGGTTTC 
CAGCTTTGGC CTAAAGAGCC ACTTAAGCAT GGAGATAGTG ACAGTCCATT GGAGGGCACC 
ATCCAAAATG GTGGAGGGGC CTCTCTCCGG AGAGTAGAGA CCTTTAACTC CCAAGGAGGA 
TTGCCTGTAC TGTATTTCAC TGGGAAGAGG GAGCGGCTGT TGCTGCGTCC AGAAGTGCTG 
GCTGAGATTC CCCGGGAGGC GTTCACAGTG GAAGCCTGGG TGAGACCAGA GGGAGGACAG 
AGCAACCCAG CCATCATTGC AGGTGTGTTT GATAACTGCT CCCACACAGT CAATGACAAG 
GGCTGGGCCC TGGGGATCCG CTCAGGGAAG GACAAGGGGT GGAGAGATGC TCGCTTCTTC 
TTTTCCCTCC GCACTGACCG GATGAAGAAA GCCACTATTG TGACTGGCCA CAGCCGTTAC 
CAGCCAGGCA TGTGGACTCA TGTAGCAGCC ACTTACGATG GACAGCATAC AGCTCTGTAT 
GTGGATGGCA CTCGAGTGGC TAGTAGTCGT GACCAGTTTG GACCGCTAAA TAGCCCTTTC 
ATGGCGTCTT GCCGCTCTTT AATCCTGGGA GGGGACAGCT CCGAGGATGG ACATTATTTC 
CGTGGTTACC TGGGCACACT GGTAATCTGG TCGACCGCCC TTTCACAAAC CCACCTCCAG 
CACAGTCCCC TGCATCCAAG TGCAGCAGAT GAGTTGACCA CCCTGATCCT GACAGCCACT 
TTCGATCCTC TGATGGAACA GTGGGCTCCA TTTAGAGATG ACTTATATCC CCGGCTGGAG 
GTTCTCCAGG ACTCTGAGTC ACAGCCTGAG ATTCTGTCAC CTTTACAACC CCCACCTTGT 
GGGCAAACAG CTTGTGACAA TGTGGAACTC ATCTCCCAGT ACAATAAGCA TGGGCCCCTT 
CGTAGAGAGA AAGTGATCCG CTACCAGGTG GTAAACATCT ATGATGATGA GGGTCTGCAT 
CCCATTGTGA GTGATCATCA GATCCGCCGT CAGCATGAGG CCCTCAACCA GGCCTTCAGC 
CGCTACAATA TCAGCTGGCA GCTGAGTGTC CACCGAGTCC ACAACTCCAC CCTGCGCCAC 
CGGGTTGTGC TTGTTAATTG TGAGCCCAGC AAGATTGGCA ATGACCACTG TGACCCTGAA 
TGTGAGCATC CACTCACCGG CTTCGATGGG GGCGATTGCC GCCTGCAGGG ACGCTGCTAC 
TCCTGGAATC GCAGGGATGG CCTCTGTCAC GTGGAGTGCA ACAACATGCT GAATGACTTC 
GATGATGGAG ACTGCTGCGA TCCGGAAGTG ACTGATGTCC GAAAGACTTG CTTTGATCCT 
GACTCACCCA AGAGGGCATA CATGAGTGTG AAGGAACTAA AGGAGGCCTT GCAGCTCAAC 
AGCACTCACT TCCTTAATGT CTACTTCGCC AGCTCAGTCC GGGAAGACCT TGCAGGTTCT 
GCCACATGGC CCTGGGACAA GGAAGCTCTC AGTCACCTGG GTGGTGTTGT CCTCAACCCA 
ACCTATTATG GCATGCTTGG CCACACCAAC ATCATGATCC ATGAGGTGGG ACATGTCCTG 
GGACTCTACC ATGTATTCAA AGGAGTCAGT GAAAGAGAAT CCTGTGATGA TCCCTGTAGA 
GAGACAGTGC CATCCATGGA GACAGGAGAC CTGTGTGCTG ACACTGCTCC CACACCCAAG 
AGCAAGCTGT GTCGGGACCC AGAGCCAGCT AATGACACCT GTGGCTTCAC CCTCTTCCCA 
GGGGCTCCGT TCAATAACTA CATGAGCTAC ACAGATGATG AGTGCACTGA CAACTTCACC 
CCTAACCAAG TGGCCCGGAT GCATTGCTAC TTGGACCTCG TATACCAGCA ATGGAGTGAA 
AGCCAAAAAC CTACCCCCAT ACCCATACCA CCCATGGTTA TTGGGCAAAC CCACAAGTCC 
CTTACTATCC ATTGGCTGCC TCCAATCAGT GGAGTTGTGT ATGATAGGGC CCCTGACAGC 
ATGTGTAGTG CCTGCACTGA AGATGGGACA TTCCGTCAGT ATGTATACAA GGCATCCTCT 
GGGCGGGTGT GTGACTCTTC AGGTTACTGG ACTCCAGAGG AGGCTGTGGG GCCTCCTGAT 
GTCGATCAAC CCTGTGAGCC TAGCTTGCAG GCCTGGAGTC CTGAGCTCCA CCTAAACCAC 
ATGAATATGA CCGTGCCCTG CCCAGCAGAA GGCTGTAGCT TGGAGTTGCT TTTTCAATAC 
CCGGTCCAGG CGGATACCCT CACCCTCTGG GTCACTTACC TCTCCATGAA CTCTTCCAAG 
GCATTCTTTG ACATAGAGAT CTTGCTAGAA CACAAAAAAT CTATGCACCT TGGACCTTTA 
AACACTTTTT GTGACATACC GCTCACCATC AAACTCCACA TGGATGAGAA GGTCATGGGA 
GCGAAAGTCT ACACCTTTGA TGAACGAATG GAAATTGATG CTGCTCTCCT GACCTCTCGG 
CCCAATAGTT CCTTGTGTTC TGGATGCAAG CCTATGAGTT ACCAGGTTCT TCGAGAGCCT 
CCTTTTTCCA GTGGCTTGCC CATGGTGGTG ACACATCCAC ACAGAAAGTT CACAGATACG 
GAGGTCATCC CTGGGCAGAT GTATCAGTAC CAAGTTCAAG CTGAAGCTGT AGGGGAACTC 
GGAGAAGCTT CACCTCCTCT GAGCCACATT CACGGAGGCC CTTACTGTGG AGATGGGAAG 
GTAGAAAGGA GTATCGGAGA GATGTGTGAT GATGGAGACC TGTTGAATGG AGATGGGTGC 
TCAAGAGCAT GTGAACTGGA GGAAGGCTTC AATTGTGCAG GGGAGCCAAG CCTTTGCTAC 
AGGTATGAGG GAGATGGGGT TTGTGAAGAT TTTGAGAAGG AAAGCATCAT CACAGACTGT 
GGCCTCCACA CTCCTGAAGG ATATTTGGAT CAGTGGGCTA GCCAGGCTTA TTCCTACCAT 
GAAGACAAGG AGAAATGTCC TGTTTCCCTG GTAACAGGAG AACCTCATTC AATGATCTGT 
ACTTCATGGC ACCCGGATTC ATCCCCATAT CATTCCTTTC CTGGCTGGTT TCCCTGTGTC 
TTCAGTCTGA AGAAAGACCA GGATGCAGGC AGTGAGCAGG CTAAAGATAG CCTGCAGAAG 
GACAATGAAA TTTGGCTTGA AGTGTGTTTC AATAGGCCAG GGGTTGCAGT GGCAATTTAC 
ATTTTCTTGG CATCGGATGG CGTGACCCCT GGGGAGCATC AGCAGGCAAC AGTGACCCTT 
TACCTGATTG ATGTCAGTGG AAGCAACCAC TCTCTTGGAA CCTATGGACT GTCGTGTCAA 
CAAAATCCAC TGGTTATCAA TGTGAGCCAT CATGTGAATG TCTACCCCCA ACATACCTCC 
TCCATGCTGC TGAATTTTTC ATCCCCACTG GTGGGCATCT CAGCAGTGGC TCTAAGGACA 
TCCTCCCAGA CCAGTTCTTC AGCTCCCAGT AACTGCATCC CAGAGCAAAA TTATGAGGGA 
AAGAGCTGTG CTCAACGACC TTGTGGAGAA CAGGGAAGAT GTGCGCCATT GCTACTCGAT 
CATACGGACA TGGTGAATTG TACCTCCAGT AGCCCAGGTC ACATGGAGTG CGCTATCACT 
TGTCAAAGGG GGTATGTCCT TCAGACCAGC AGTGGGCAGT ACCTGAGGAA CATGCAGAAA 
GAGATTCTCC TTACTTGTTC CTCTGGCCAC TGGGACAAGG ATGTGATCTG CAAGCGTCTT 
GACTGTGGTG TCCCAGACTC CTCCTTGGTG AATTATGCAA ACTTCTCCTG CTTGGAGGGT 
ACAGACTTTC TGAAACAATG TTCCATCTCG TGTGTCCCAC CGGCTAAGCT CCAAGGACTG 
AACCCATGGC TGACATGTCT TGAAGATGGA CTCTGGTCTC TCCCTGAAGT CTACTGCAAG 
TTGGAATGTG AAGCTCCTCC AGTTATTCCC AATGCCAATC TGCTCCTGCC ACATTTCCTG 
GAAGGCAACC ATGATGTGGG CACCATCTGC AAATATGAGT GCAAACCAGG CTACTATGTG 
AAGGAGACTT CAGGAAGTCA AGGCAAGAAC AAGTTCCTGA AGATCCAGTG CCTGGATGAT 
GGAAGCTGGG AACAAGGGAG CTGCGTCCCC GTGGTTTGTG AGCCTCCTCC TCCGGTGTTT 
GAAGGCATGT ACGAATGTAC TGATGGCTTC AAGCTGGACA GCCAGTGTGT GCTCAACTGT 
AACCAGGAAA CGGAAGGGAT TCCCATCCTC TGCACTAAAG AGGGGTTGTG GACCCAGGAA 
TTCAAGTTGT GTGAAAACCT GCAAGGAGAA TGCCCACCAC CCACCTCAGA GCTGAATTCT 
GTGGAGTACA AGTGTGGACA GGGATATGGG ATCGGTGCAG TGTGCTCCCC ATCATGTGTA 
ATCCCCCCTA GTGACCCTGT GATACTACCA GAGAATGTGA CTACTGACAC TTTGGAGCAC 
TGGATGGAAC CTGTCAAAGT CCAGAGTATC GTGTGTACCG GCAGGCGCCA GTGGCACCCA 
GATCCCTCTC TGGTGCACTG CATCCAGTCA TGTGAGCCTT TCCAAGCAGA TGGTTGGTGT 
GACACTATTA ATAACCGGGC CTACTGCAAC TATGACGGGG GAGACTGCTG CTCTTCCACA 
CTCTCTTCCA AGAAGGTCAT TCCATTTGCT GCTGATTGTG ACTTGGATGA ATGCACCTGT 
CGAGACCCCA AGGCAGAAGA AAACCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

PAPP-A2 a new key regulator of growth.
Endokrynologia Polska68(6)682-691(2017)
Banaszak-Ziemska M,Niedziela M

PAPP-A2 expression by osteoblasts is required for normal postnatal growth in mice.
Growth hormone & IGF research : official journal of the Growth Hormone Research Society and the International IGF Research Society25(6)274-80(2015 Dec)
Amiri N,Christians JK

Pappa2 deletion in mice affects male but not female fertility.
Reproductive biology and endocrinology : RB&E13109(2015 Sep)
Christians JK,King AY,Rogowska MD,Hessels SM

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Pappa2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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