Clca1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Clca1
Entrez Gene ID	23844
Full Name	chloride channel accessory 1
Synonyms	Clca2,Clca3,gob-5,gob5
General protein information	Preferred Names chloride channel accessory 1 Names calcium-activated chloride channel regulator 1 calcium-activated chloride channel family member 3 chloride channel calcium activated 1 chloride channel calcium activated 2 chloride channel calcium activated 3 mCLCA3 protein gob-5
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 3 Map Location: 3 H2\|3 69.26 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_017474.2	NP_059502.1	calcium-activated chloride channel regulator 1 precursor
XM_006501444.1	XP_006501507.1	calcium-activated chloride channel regulator 1 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CLCA1	XP_001143250.1
	CLCA1	NP_001028084.1
Mus musculus (house mouse)	Clca3	NP_059502.1
Canis lupus familiaris (dog)	CLCA1	XP_547299.2
Bos taurus (cattle)	CLCA1	NP_001193105.1
Homo sapiens (human)	CLCA1	NP_001276.2
Rattus norvegicus (Norway rat)	Clca1	NP_001100919.1

Gene Ontology
Bibliography

Related articles in PubMed

The Lymphatic Endothelial mCLCA1 Antibody Induces Proliferation and Growth of Lymph Node Lymphatic Sinuses.
Jordan-Williams KL, Ramanujam N, Farr AG, Ruddell A
PloS one11(5)e0156079(2016)

Integrin-Alpha IIb Identifies Murine Lymph Node Lymphatic Endothelial Cells Responsive to RANKL.
Cordeiro OG, Chypre M, Brouard N, Rauber S, Alloush F, Romera-Hernandez M, B?n?zech C, Li Z, Eckly A, Coles MC, Rot A, Yagita H, L?on C, Ludewig B, Cupedo T, Lanza F, Mueller CG
PloS one11(3)e0151848(2016)

The Goblet Cell Protein Clca1 (Alias mClca3 or Gob-5) Is Not Required for Intestinal Mucus Synthesis, Structure and Barrier Function in Naive or DSS-Challenged Mice.
Erickson NA, Nystr?m EE, Mundhenk L, Arike L, Glauben R, Heimesaat MM, Fischer A, Bereswill S, Birchenough GM, Gruber AD, Johansson ME
PloS one10(7)e0131991(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These findings demonstrate that 10.1.1 Ab rapidly and specifically induces proliferation and growth of Lymph Node lymphatic sinuses and stroma, suggesting a key role of mCLCA1 in coordinating Lymph Node remodeling during immune responses.
Title: The Lymphatic Endothelial mCLCA1 Antibody Induces Proliferation and Growth of Lymph Node Lymphatic Sinuses.

Clca1 is not required for mucus synthesis, structure and barrier function in the murine colon.
Title: The Goblet Cell Protein Clca1 (Alias mClca3 or Gob-5) Is Not Required for Intestinal Mucus Synthesis, Structure and Barrier Function in Naive or DSS-Challenged Mice.

mCLCA3 appears to modulate leukocyte response via IL-17 and murine CXCL-8 homologs in acute Staphylococcus aureus pneumonia which is well in line with the proposed function of hCLCA1 as a signaling molecule acting on alveolar macrophages.
Title: mCLCA3 modulates IL-17 and CXCL-1 induction and leukocyte recruitment in murine Staphylococcus aureus pneumonia.

Findings suggest that increased expression of a single-gene, mCLCA3, could simulate an asthma attack, and its mechanism may involve mCLCA3 overexpression up-regulating IL-13 expression.
Title: Murine calcium-activated chloride channel family member 3 induces asthmatic airway inflammation independently of allergen exposure.

PA-LPS-induced MUC5AC and Clca3 expression is partly through Duox1
Title: P. aeruginosa lipopolysaccharide-induced MUC5AC and CLCA3 expression is partly through Duox1 in vitro and in vivo.

The following Clca1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Clca1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu31887	XM_006501444.1 Latest version!	Mus musculus chloride channel accessory 1 (Clca1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OMu17804	NM_017474.2 Latest version!	Mus musculus chloride channel accessory 1 (Clca1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$538.30 $769.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Clca1 ( XM_006501444.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501507.1 Mus musculus chloride channel accessory 1 (Clca1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu31887
Clone ID Related Accession (Same CDS sequence)	XM_006501444.1
Accession Version	XM_006501444.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	calcium-activated chloride channel regulator 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGCCC AGCCACCTGC ACCCACCTTC TCACTGCTGC AAATTGGACA AAGAATTGTG 
TGCTTAGTTC TTGATAAGTC CGGGAGCATG CTGAACGATG ATCGTCTTAA CCGAATGAAT 
CAGGCAAGCC GGCTTTTCCT GCTGCAGACT GTGGAGCAGG GATCCTGGGT CGGGATGGTG 
ACCTTTGACA GTGCTGCCTA TGTACAAAGC GAACTCAAAC AGTTAAACAG TGGTGCTGAC 
AGAGATCTGC TGATCAAGCA CTTACCCACA GTATCTGCAG GAGGGACATC TATATGCTCT 
GGCCTTCGGA CAGCATTTAC AGTGATAAAG AAGAAGTATC CAACTGATGG ATCTGAAATT 
GTGCTGCTGA CCGATGGGGA GGACAACACC ATTAGCAGCT GCTTTGACCT GGTGAAGCAG 
AGCGGGGCCA TCATCCATAC AGTGGCCCTG GGACCGGCTG CCGCTAAAGA GCTTGAGCAG 
CTGTCCAAAA TGACAGGAGG CCTGCAGACA TACTCTTCGG ATCAGGTTCA GAACAATGGT 
CTTGTTGATG CTTTCGCAGC ACTCTCCTCA GGAAATGCGG CGATCGCTCA GCACTCCATC 
CAGCTGGAGA GCAGGGGAGT TAATCTCCAG AATAACCAAT GGATGAATGG CTCAGTGATC 
GTGGACAGCT CGGTGGGCAA GGACACCTTG TTTCTTATCA CCTGGACAAC GCATCCTCCT 
ACAATATTTA TCTGGGATCC CAGCGGAGTG GAACAAAATG GTTTTATACT AGACACAACC 
ACTAAGGTGG CCTACCTCCA AGTCCCAGGC ACGGCTAAGG TTGGCTTTTG GAAATACAGC 
ATTCAAGCGA GCTCACAGAC TCTCACCTTG ACTGTCACCT CCCGTGCAGC AAGTGCTACA 
CTGCCTCCTA TTACAGTGAC CCCGGTAGTG AATAAGAACA CAGGGAAATT CCCCAGCCCT 
GTAACAGTGT ATGCAAGCAT TCGCCAAGGA GCCTCGCCTA TTCTCAGGGC CAGCGTCACA 
GCCTTGATTG AATCTGTGAA TGGAAAAACA GTAACCCTGG AATTACTGGA TAACGGAGCA 
GGTGCCGATG CCACCAAGAA TGATGGTGTC TACTCAAGGT TTTTTACAGC TTTTGATGCA 
AATGGTAGAT ACAGCGTTAA AATATGGGCT CTGGGAGGAG TCACTTCAGA CAGACAGAGA 
GCAGCACCTC CGAAGAACAG AGCCATGTAC ATAGATGGCT GGATTGAGGA TGGTGAAGTA 
AGAATGAACC CACCACGTCC TGAAACTAGT TATGTTCAAG ACAAGCAGCT GTGCTTCAGC 
AGGACATCTT CAGGGGGATC GTTTGTGGCC ACCAATGTCC CCGCAGCAGC TCCCATTCCT 
GACCTCTTTC CACCCTGTCA AATCACTGAC CTGAAGGCCA GCATCCAAGG GCAGAACCTG 
GTGAATCTGA CGTGGACGGC TCCTGGGGAT GACTACGACC ACGGGAGAGC TTCCAACTAC 
ATCATCCGAA TGAGCACCAG TATCGTTGAT CTCAGGGACC ACTTCAACAC CTCACTCCAA 
GTGAACACTA CCGGTCTTAT CCCCAAAGAG GCCAGCTCTG AGGAAATCTT TGAGTTTGAA 
CTGGGAGGCA ACACTTTTGG AAATGGCACA GATATCTTCA TTGCTATCCA GGCTGTGGAT 
AAGTCCAATC TGAAATCAGA AATCTCCAAC ATTGCACGGG TGTCTGTGTT CATCCCCGCT 
CAGGAGCCGC CCATTCCCGA AGACTCAACT CCCCCTTGTC CTGACATCAG CATCAACAGC 
ACCATTCCTG GCATCCACGT GCTGAAGATA ATGTGGAAGT GGCTAGGGGA AATGCAGGTG 
ACACTAGGTT TGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501507.1
CDS	605..2482
Translation	MTAQPPAPTFSLLQIGQRIVCLVLDKSGSMLNDDRLNRMNQASRLFLLQTVEQGSWVGMVTFDSAAYVQSELKQLNSGADRDLLIKHLPTVSAGGTSICSGLRTAFTVIKKKYPTDGSEIVLLTDGEDNTISSCFDLVKQSGAIIHTVALGPAAAKELEQLSKMTGGLQTYSSDQVQNNGLVDAFAALSSGNAAIAQHSIQLESRGVNLQNNQWMNGSVIVDSSVGKDTLFLITWTTHPPTIFIWDPSGVEQNGFILDTTTKVAYLQVPGTAKVGFWKYSIQASSQTLTLTVTSRAASATLPPITVTPVVNKNTGKFPSPVTVYASIRQGASPILRASVTALIESVNGKTVTLELLDNGAGADATKNDGVYSRFFTAFDANGRYSVKIWALGGVTSDRQRAAPPKNRAMYIDGWIEDGEVRMNPPRPETSYVQDKQLCFSRTSSGGSFVATNVPAAAPIPDLFPPCQITDLKASIQGQNLVNLTWTAPGDDYDHGRASNYIIRMSTSIVDLRDHFNTSLQVNTTGLIPKEASSEEIFEFELGGNTFGNGTDIFIAIQAVDKSNLKSEISNIARVSVFIPAQEPPIPEDSTPPCPDISINSTIPGIHVLKIMWKWLGEMQVTLGLH

Target ORF information:

RefSeq Version	XM_006501444.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus chloride channel accessory 1 (Clca1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501444.1

ORF Insert Sequence:

ATGACAGCCC AGCCACCTGC ACCCACCTTC TCACTGCTGC AAATTGGACA AAGAATTGTG 
TGCTTAGTTC TTGATAAGTC CGGGAGCATG CTGAACGATG ATCGTCTTAA CCGAATGAAT 
CAGGCAAGCC GGCTTTTCCT GCTGCAGACT GTGGAGCAGG GATCCTGGGT CGGGATGGTG 
ACCTTTGACA GTGCTGCCTA TGTACAAAGC GAACTCAAAC AGTTAAACAG TGGTGCTGAC 
AGAGATCTGC TGATCAAGCA CTTACCCACA GTATCTGCAG GAGGGACATC TATATGCTCT 
GGCCTTCGGA CAGCATTTAC AGTGATAAAG AAGAAGTATC CAACTGATGG ATCTGAAATT 
GTGCTGCTGA CCGATGGGGA GGACAACACC ATTAGCAGCT GCTTTGACCT GGTGAAGCAG 
AGCGGGGCCA TCATCCATAC AGTGGCCCTG GGACCGGCTG CCGCTAAAGA GCTTGAGCAG 
CTGTCCAAAA TGACAGGAGG CCTGCAGACA TACTCTTCGG ATCAGGTTCA GAACAATGGT 
CTTGTTGATG CTTTCGCAGC ACTCTCCTCA GGAAATGCGG CGATCGCTCA GCACTCCATC 
CAGCTGGAGA GCAGGGGAGT TAATCTCCAG AATAACCAAT GGATGAATGG CTCAGTGATC 
GTGGACAGCT CGGTGGGCAA GGACACCTTG TTTCTTATCA CCTGGACAAC GCATCCTCCT 
ACAATATTTA TCTGGGATCC CAGCGGAGTG GAACAAAATG GTTTTATACT AGACACAACC 
ACTAAGGTGG CCTACCTCCA AGTCCCAGGC ACGGCTAAGG TTGGCTTTTG GAAATACAGC 
ATTCAAGCGA GCTCACAGAC TCTCACCTTG ACTGTCACCT CCCGTGCAGC AAGTGCTACA 
CTGCCTCCTA TTACAGTGAC CCCGGTAGTG AATAAGAACA CAGGGAAATT CCCCAGCCCT 
GTAACAGTGT ATGCAAGCAT TCGCCAAGGA GCCTCGCCTA TTCTCAGGGC CAGCGTCACA 
GCCTTGATTG AATCTGTGAA TGGAAAAACA GTAACCCTGG AATTACTGGA TAACGGAGCA 
GGTGCCGATG CCACCAAGAA TGATGGTGTC TACTCAAGGT TTTTTACAGC TTTTGATGCA 
AATGGTAGAT ACAGCGTTAA AATATGGGCT CTGGGAGGAG TCACTTCAGA CAGACAGAGA 
GCAGCACCTC CGAAGAACAG AGCCATGTAC ATAGATGGCT GGATTGAGGA TGGTGAAGTA 
AGAATGAACC CACCACGTCC TGAAACTAGT TATGTTCAAG ACAAGCAGCT GTGCTTCAGC 
AGGACATCTT CAGGGGGATC GTTTGTGGCC ACCAATGTCC CCGCAGCAGC TCCCATTCCT 
GACCTCTTTC CACCCTGTCA AATCACTGAC CTGAAGGCCA GCATCCAAGG GCAGAACCTG 
GTGAATCTGA CGTGGACGGC TCCTGGGGAT GACTACGACC ACGGGAGAGC TTCCAACTAC 
ATCATCCGAA TGAGCACCAG TATCGTTGAT CTCAGGGACC ACTTCAACAC CTCACTCCAA 
GTGAACACTA CCGGTCTTAT CCCCAAAGAG GCCAGCTCTG AGGAAATCTT TGAGTTTGAA 
CTGGGAGGCA ACACTTTTGG AAATGGCACA GATATCTTCA TTGCTATCCA GGCTGTGGAT 
AAGTCCAATC TGAAATCAGA AATCTCCAAC ATTGCACGGG TGTCTGTGTT CATCCCCGCT 
CAGGAGCCGC CCATTCCCGA AGACTCAACT CCCCCTTGTC CTGACATCAG CATCAACAGC 
ACCATTCCTG GCATCCACGT GCTGAAGATA ATGTGGAAGT GGCTAGGGGA AATGCAGGTG 
ACACTAGGTT TGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Clca1 ( NM_017474.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_059502.1 Mus musculus chloride channel accessory 1 (Clca1), mRNA.

Price & Availability↑

CloneID	OMu17804
Clone ID Related Accession (Same CDS sequence)	NM_017474.2
Accession Version	NM_017474.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2742bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Mus musculus(house mouse)
Product	calcium-activated chloride channel regulator 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK033560.1 and AK008659.2. On Aug 13, 2009 this sequence version replaced NM_017474.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK033560.1, AB017156.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849377 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAATCTT TGAAGAGTCC TGTCTTCCTC TTGATCCTCC ACCTTCTGGA AGGAGTTCTG 
AGTGAGTCCC TCATCCAACT GAACAACAAC GGCTATGAGG GCATCGTCAT CGCCATAGAC 
CACGACGTGC CGGAAGATGA AGCCCTCATT CAACACATAA AGGACATGGT GACTCAGGCC 
TCTCCATACC TGTTTGAAGC TACAGGAAAA AGATTTTACT TCAAAAATGT TGCCATTTTG 
ATTCCCGAGA GCTGGAAGGC AAAGCCTGAA TATACGAGGC CAAAACTTGA AACCTTCAAA 
AACGCTGATG TCCTTGTATC AACAACCAGC CCTCTAGGCA ATGATGAGCC CTACACCGAA 
CATATAGGAG CATGTGGAGA AAAGGGGATC AGGATTCACC TGACTCCTGA CTTCTTAGCA 
GGAAAGAAGC TGACTCAGTA TGGGCCACAA GACAGGACCT TTGTCCATGA GTGGGCTCAC 
TTCCGATGGG GAGTGTTTAA TGAATACAAC AACGACGAGA AGTTCTACTT ATCCAAAGGA 
AAACCCCAAG CAGTGAGGTG TTCAGCAGCC ATTACCGGTA AAAATCAAGT TCGTCGGTGC 
CAGGGAGGCA GTTGTATCAC TAACGGAAAG TGTGTAATCG ACAGAGTAAC GGGACTGTAT 
AAAGACAATT GTGTATTTGT ACCAGATCCA CACCAAAACG AGAAGGCTTC CATCATGTTT 
AACCAAAATA TCAATTCTGT GGTTGAATTC TGTACAGAAA AAAATCACAA TCAAGAAGCC 
CCAAATGACC AAAACCAACG ATGCAATCTC CGAAGCACGT GGGAAGTCAT CCAGGAATCT 
GAGGACTTCA AGCAAACCAC TCCCATGACA GCCCAGCCAC CTGCACCCAC CTTCTCACTG 
CTGCAAATTG GACAAAGAAT TGTGTGCTTA GTTCTTGATA AGTCCGGGAG CATGCTGAAC 
GATGATCGTC TTAACCGAAT GAATCAGGCA AGCCGGCTTT TCCTGCTGCA GACTGTGGAG 
CAGGGATCCT GGGTCGGGAT GGTGACCTTT GACAGTGCTG CCTATGTACA AAGCGAACTC 
AAACAGTTAA ACAGTGGTGC TGACAGAGAT CTGCTGATCA AGCACTTACC CACAGTATCT 
GCAGGAGGGA CATCTATATG CTCTGGCCTT CGGACAGCAT TTACAGTGAT AAAGAAGAAG 
TATCCAACTG ATGGATCTGA AATTGTGCTG CTGACCGATG GGGAGGACAA CACCATTAGC 
AGCTGCTTTG ACCTGGTGAA GCAGAGCGGG GCCATCATCC ATACAGTGGC CCTGGGACCG 
GCTGCCGCTA AAGAGCTTGA GCAGCTGTCC AAAATGACAG GAGGCCTGCA GACATACTCT 
TCGGATCAGG TTCAGAACAA TGGTCTTGTT GATGCTTTCG CAGCACTCTC CTCAGGAAAT 
GCGGCGATCG CTCAGCACTC CATCCAGCTG GAGAGCAGGG GAGTTAATCT CCAGAATAAC 
CAATGGATGA ATGGCTCAGT GATCGTGGAC AGCTCGGTGG GCAAGGACAC CTTGTTTCTT 
ATCACCTGGA CAACGCATCC TCCTACAATA TTTATCTGGG ATCCCAGCGG AGTGGAACAA 
AATGGTTTTA TACTAGACAC AACCACTAAG GTGGCCTACC TCCAAGTCCC AGGCACGGCT 
AAGGTTGGCT TTTGGAAATA CAGCATTCAA GCGAGCTCAC AGACTCTCAC CTTGACTGTC 
ACCTCCCGTG CAGCAAGTGC TACACTGCCT CCTATTACAG TGACCCCGGT AGTGAATAAG 
AACACAGGGA AATTCCCCAG CCCTGTAACA GTGTATGCAA GCATTCGCCA AGGAGCCTCG 
CCTATTCTCA GGGCCAGCGT CACAGCCTTG ATTGAATCTG TGAATGGAAA AACAGTAACC 
CTGGAATTAC TGGATAACGG AGCAGGTGCC GATGCCACCA AGAATGATGG TGTCTACTCA 
AGGTTTTTTA CAGCTTTTGA TGCAAATGGT AGATACAGCG TTAAAATATG GGCTCTGGGA 
GGAGTCACTT CAGACAGACA GAGAGCAGCA CCTCCGAAGA ACAGAGCCAT GTACATAGAT 
GGCTGGATTG AGGATGGTGA AGTAAGAATG AACCCACCAC GTCCTGAAAC TAGTTATGTT 
CAAGACAAGC AGCTGTGCTT CAGCAGGACA TCTTCAGGGG GATCGTTTGT GGCCACCAAT 
GTCCCCGCAG CAGCTCCCAT TCCTGACCTC TTTCCACCCT GTCAAATCAC TGACCTGAAG 
GCCAGCATCC AAGGGCAGAA CCTGGTGAAT CTGACGTGGA CGGCTCCTGG GGATGACTAC 
GACCACGGGA GAGCTTCCAA CTACATCATC CGAATGAGCA CCAGTATCGT TGATCTCAGG 
GACCACTTCA ACACCTCACT CCAAGTGAAC ACTACCGGTC TTATCCCCAA AGAGGCCAGC 
TCTGAGGAAA TCTTTGAGTT TGAACTGGGA GGCAACACTT TTGGAAATGG CACAGATATC 
TTCATTGCTA TCCAGGCTGT GGATAAGTCC AATCTGAAAT CAGAAATCTC CAACATTGCA 
CGGGTGTCTG TGTTCATCCC CGCTCAGGAG CCGCCCATTC CCGAAGACTC AACTCCCCCT 
TGTCCTGACA TCAGCATCAA CAGCACCATT CCTGGCATCC ACGTGCTGAA GATAATGTGG 
AAGTGGCTAG GGGAAATGCA GGTGACACTA GGTTTGCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_059502.1
CDS	32..2773
Translation	MESLKSPVFLLILHLLEGVLSESLIQLNNNGYEGIVIAIDHDVPEDEALIQHIKDMVTQASPYLFEATGKRFYFKNVAILIPESWKAKPEYTRPKLETFKNADVLVSTTSPLGNDEPYTEHIGACGEKGIRIHLTPDFLAGKKLTQYGPQDRTFVHEWAHFRWGVFNEYNNDEKFYLSKGKPQAVRCSAAITGKNQVRRCQGGSCITNGKCVIDRVTGLYKDNCVFVPDPHQNEKASIMFNQNINSVVEFCTEKNHNQEAPNDQNQRCNLRSTWEVIQESEDFKQTTPMTAQPPAPTFSLLQIGQRIVCLVLDKSGSMLNDDRLNRMNQASRLFLLQTVEQGSWVGMVTFDSAAYVQSELKQLNSGADRDLLIKHLPTVSAGGTSICSGLRTAFTVIKKKYPTDGSEIVLLTDGEDNTISSCFDLVKQSGAIIHTVALGPAAAKELEQLSKMTGGLQTYSSDQVQNNGLVDAFAALSSGNAAIAQHSIQLESRGVNLQNNQWMNGSVIVDSSVGKDTLFLITWTTHPPTIFIWDPSGVEQNGFILDTTTKVAYLQVPGTAKVGFWKYSIQASSQTLTLTVTSRAASATLPPITVTPVVNKNTGKFPSPVTVYASIRQGASPILRASVTALIESVNGKTVTLELLDNGAGADATKNDGVYSRFFTAFDANGRYSVKIWALGGVTSDRQRAAPPKNRAMYIDGWIEDGEVRMNPPRPETSYVQDKQLCFSRTSSGGSFVATNVPAAAPIPDLFPPCQITDLKASIQGQNLVNLTWTAPGDDYDHGRASNYIIRMSTSIVDLRDHFNTSLQVNTTGLIPKEASSEEIFEFELGGNTFGNGTDIFIAIQAVDKSNLKSEISNIARVSVFIPAQEPPIPEDSTPPCPDISINSTIPGIHVLKIMWKWLGEMQVTLGLH

Target ORF information:

RefSeq Version	NM_017474.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus chloride channel accessory 1 (Clca1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017474.2

ORF Insert Sequence:

ATGGAATCTT TGAAGAGTCC TGTCTTCCTC TTGATCCTCC ACCTTCTGGA AGGAGTTCTG 
AGTGAGTCCC TCATCCAACT GAACAACAAC GGCTATGAGG GCATCGTCAT CGCCATAGAC 
CACGACGTGC CGGAAGATGA AGCCCTCATT CAACACATAA AGGACATGGT GACTCAGGCC 
TCTCCATACC TGTTTGAAGC TACAGGAAAA AGATTTTACT TCAAAAATGT TGCCATTTTG 
ATTCCCGAGA GCTGGAAGGC AAAGCCTGAA TATACGAGGC CAAAACTTGA AACCTTCAAA 
AACGCTGATG TCCTTGTATC AACAACCAGC CCTCTAGGCA ATGATGAGCC CTACACCGAA 
CATATAGGAG CATGTGGAGA AAAGGGGATC AGGATTCACC TGACTCCTGA CTTCTTAGCA 
GGAAAGAAGC TGACTCAGTA TGGGCCACAA GACAGGACCT TTGTCCATGA GTGGGCTCAC 
TTCCGATGGG GAGTGTTTAA TGAATACAAC AACGACGAGA AGTTCTACTT ATCCAAAGGA 
AAACCCCAAG CAGTGAGGTG TTCAGCAGCC ATTACCGGTA AAAATCAAGT TCGTCGGTGC 
CAGGGAGGCA GTTGTATCAC TAACGGAAAG TGTGTAATCG ACAGAGTAAC GGGACTGTAT 
AAAGACAATT GTGTATTTGT ACCAGATCCA CACCAAAACG AGAAGGCTTC CATCATGTTT 
AACCAAAATA TCAATTCTGT GGTTGAATTC TGTACAGAAA AAAATCACAA TCAAGAAGCC 
CCAAATGACC AAAACCAACG ATGCAATCTC CGAAGCACGT GGGAAGTCAT CCAGGAATCT 
GAGGACTTCA AGCAAACCAC TCCCATGACA GCCCAGCCAC CTGCACCCAC CTTCTCACTG 
CTGCAAATTG GACAAAGAAT TGTGTGCTTA GTTCTTGATA AGTCCGGGAG CATGCTGAAC 
GATGATCGTC TTAACCGAAT GAATCAGGCA AGCCGGCTTT TCCTGCTGCA GACTGTGGAG 
CAGGGATCCT GGGTCGGGAT GGTGACCTTT GACAGTGCTG CCTATGTACA AAGCGAACTC 
AAACAGTTAA ACAGTGGTGC TGACAGAGAT CTGCTGATCA AGCACTTACC CACAGTATCT 
GCAGGAGGGA CATCTATATG CTCTGGCCTT CGGACAGCAT TTACAGTGAT AAAGAAGAAG 
TATCCAACTG ATGGATCTGA AATTGTGCTG CTGACCGATG GGGAGGACAA CACCATTAGC 
AGCTGCTTTG ACCTGGTGAA GCAGAGCGGG GCCATCATCC ATACAGTGGC CCTGGGACCG 
GCTGCCGCTA AAGAGCTTGA GCAGCTGTCC AAAATGACAG GAGGCCTGCA GACATACTCT 
TCGGATCAGG TTCAGAACAA TGGTCTTGTT GATGCTTTCG CAGCACTCTC CTCAGGAAAT 
GCGGCGATCG CTCAGCACTC CATCCAGCTG GAGAGCAGGG GAGTTAATCT CCAGAATAAC 
CAATGGATGA ATGGCTCAGT GATCGTGGAC AGCTCGGTGG GCAAGGACAC CTTGTTTCTT 
ATCACCTGGA CAACGCATCC TCCTACAATA TTTATCTGGG ATCCCAGCGG AGTGGAACAA 
AATGGTTTTA TACTAGACAC AACCACTAAG GTGGCCTACC TCCAAGTCCC AGGCACGGCT 
AAGGTTGGCT TTTGGAAATA CAGCATTCAA GCGAGCTCAC AGACTCTCAC CTTGACTGTC 
ACCTCCCGTG CAGCAAGTGC TACACTGCCT CCTATTACAG TGACCCCGGT AGTGAATAAG 
AACACAGGGA AATTCCCCAG CCCTGTAACA GTGTATGCAA GCATTCGCCA AGGAGCCTCG 
CCTATTCTCA GGGCCAGCGT CACAGCCTTG ATTGAATCTG TGAATGGAAA AACAGTAACC 
CTGGAATTAC TGGATAACGG AGCAGGTGCC GATGCCACCA AGAATGATGG TGTCTACTCA 
AGGTTTTTTA CAGCTTTTGA TGCAAATGGT AGATACAGCG TTAAAATATG GGCTCTGGGA 
GGAGTCACTT CAGACAGACA GAGAGCAGCA CCTCCGAAGA ACAGAGCCAT GTACATAGAT 
GGCTGGATTG AGGATGGTGA AGTAAGAATG AACCCACCAC GTCCTGAAAC TAGTTATGTT 
CAAGACAAGC AGCTGTGCTT CAGCAGGACA TCTTCAGGGG GATCGTTTGT GGCCACCAAT 
GTCCCCGCAG CAGCTCCCAT TCCTGACCTC TTTCCACCCT GTCAAATCAC TGACCTGAAG 
GCCAGCATCC AAGGGCAGAA CCTGGTGAAT CTGACGTGGA CGGCTCCTGG GGATGACTAC 
GACCACGGGA GAGCTTCCAA CTACATCATC CGAATGAGCA CCAGTATCGT TGATCTCAGG 
GACCACTTCA ACACCTCACT CCAAGTGAAC ACTACCGGTC TTATCCCCAA AGAGGCCAGC 
TCTGAGGAAA TCTTTGAGTT TGAACTGGGA GGCAACACTT TTGGAAATGG CACAGATATC 
TTCATTGCTA TCCAGGCTGT GGATAAGTCC AATCTGAAAT CAGAAATCTC CAACATTGCA 
CGGGTGTCTG TGTTCATCCC CGCTCAGGAG CCGCCCATTC CCGAAGACTC AACTCCCCCT 
TGTCCTGACA TCAGCATCAA CAGCACCATT CCTGGCATCC ACGTGCTGAA GATAATGTGG 
AAGTGGCTAG GGGAAATGCA GGTGACACTA GGTTTGCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Lymphatic Endothelial mCLCA1 Antibody Induces Proliferation and Growth of Lymph Node Lymphatic Sinuses.
PloS one11(5)e0156079(2016)
Jordan-Williams KL,Ramanujam N,Farr AG,Ruddell A

Integrin-Alpha IIb Identifies Murine Lymph Node Lymphatic Endothelial Cells Responsive to RANKL.
PloS one11(3)e0151848(2016)
Cordeiro OG,Chypre M,Brouard N,Rauber S,Alloush F,Romera-Hernandez M,B?n?zech C,Li Z,Eckly A,Coles MC,Rot A,Yagita H,L?on C,Ludewig B,Cupedo T,Lanza F,Mueller CG

The Goblet Cell Protein Clca1 (Alias mClca3 or Gob-5) Is Not Required for Intestinal Mucus Synthesis, Structure and Barrier Function in Naive or DSS-Challenged Mice.
PloS one10(7)e0131991(2015)
Erickson NA,Nystr?m EE,Mundhenk L,Arike L,Glauben R,Heimesaat MM,Fischer A,Bereswill S,Birchenough GM,Gruber AD,Johansson ME