BACE1 cDNA ORF clone, Mus musculus(House mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	BACE1
Entrez Gene ID	23821
Full Name	beta-site APP cleaving enzyme 1
Synonyms	ASP2,Bace,C76936
General protein information	Preferred Names beta-site APP cleaving enzyme 1 Names beta-secretase 1 APP beta-secretase asp 2 aspartyl protease 2 beta-site amyloid precursor protein cleaving enzyme 1 memapsin-2 membrane-associated aspartic protease 2
Gene Type	protein-coding
Organism	Mus musculus(House mouse)
Genome	Chromosome: 9 Map Location: 9\|9 A5.2
Summary	This gene encodes a member of the peptidase A1 family of aspartic proteases. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protease. This transmembrane protease catalyzes the first step in the formation of amyloid beta peptide from amyloid precursor protein. Amyloid beta peptides are the main constituent of amyloid beta plaques, which accumulate in the brains of human Alzheimer's disease patients. Homozygous knockout mice for this gene exhibit a wide range of nervous system defects, growth retardation, metabolic abnormalities, and increased neonatal lethality. [provided by RefSeq, Nov 2015].

mRNA and Protein(s)

mRNA	Protein	Name
XM_011242513.3	XP_011240815.1	beta-secretase 1 isoform X2
NM_001145947.2	NP_001139419.1	beta-secretase 1 isoform 2 precursor
XM_011242513.2	XP_011240815.1	beta-secretase 1 isoform X2
NM_011792.6	NP_035922.4	beta-secretase 1 isoform 1 preproprotein
XM_011242512.2	XP_011240814.1	beta-secretase 1 isoform X1
XM_011242512.3	XP_011240814.1	beta-secretase 1 isoform X1
NM_011792.5	NP_035922.4	beta-secretase 1 isoform 1 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	BACE1	NP_036236.1
Gallus gallus (chicken)	BACE1	XP_417908.3
Danio rerio (zebrafish)	bace1	NP_991267.1
Xenopus tropicalis (tropical clawed frog)	bace1	NP_001072485.1
Bos taurus (cattle)	BACE1	NP_001039996.1
Mus musculus (house mouse)	Bace1	NP_035922.4
Rattus norvegicus (Norway rat)	Bace1	NP_062077.1
Pan troglodytes (chimpanzee)	BACE1	XP_001158428.1
	BACE1	XP_001094263.1
Canis lupus familiaris (dog)	BACE1	XP_546508.3

Gene Ontology
Bibliography

Related articles in PubMed

The normal and pathologic roles of the Alzheimer's ?-secretase, BACE1.
Kandalepas PC, Vassar R
Current Alzheimer research11(5)441-9(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In conclusion, glucocorticoid couples mGR with Galphas and triggers cAMP-PKA-CREB axis dependent on the lipid raft to stimulate BACE1 upregulation and Abeta generation.SIGNIFICANCE STATEMENT Patients with Alzheimer's disease (AD) have been growing sharply and stress is considered as the major environment factor of AD.
Title: Membrane-Associated Effects of Glucocorticoid on BACE1 Upregulation and A? Generation: Involvement of Lipid Raft-Mediated CREB Activation.

beta-site APP cleaving enzyme 1 (BACE1) is essential for amyloid beta protein production. We discovered that A673V mutation shifted the BACE1 cleavage site from the Glu(11) to the Asp(1) site, resulting in much higher C99 level and C99/C89 ratio.
Title: BACE1 Cleavage Site Selection Critical for Amyloidogenesis and Alzheimer's Pathogenesis.

Here, we demonstrate that Snapin-mediated retrograde transport plays a critical role in removing BACE1 from presynaptic terminals toward the soma, thus reducing synaptic Abeta production. Adeno-associated virus-mediated Snapin overexpression in the hippocampus of mutant hAPP mice significantly decreases synaptic Abeta levels, attenuates synapse loss, and thus rescues cognitive deficits. Our study uncovers a new pathway...
Title: Regulation of Synaptic Amyloid-? Generation through BACE1 Retrograde Transport in a Mouse Model of Alzheimer's Disease.

study provides new insights into autophagy-mediated regulation of BACE1 turnover and APP processing, thus building a foundation for future development of potential Alzheimer's disease therapeutic strategies.
Title: Autophagy-mediated Regulation of BACE1 Protein Trafficking and Degradation.

Results indicate that SNX4-mediated regulation of the steady-state levels and trafficking of BACE1, as well as the subsequent increase in BACE1-mediated cleavage, may be relevant to Alzheimer's disease progression.
Title: Sorting nexin-4 regulates ?-amyloid production by modulating ?-site-activating cleavage enzyme-1.

The following BACE1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BACE1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu18995	NM_001145947.2 Latest version!	Mus musculus beta-site APP cleaving enzyme 1 (Bace1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu69120	XM_011242512.3 Latest version!	Mus musculus beta-site APP cleaving enzyme 1 (Bace1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu69121	XM_011242513.3 Latest version!	Mus musculus beta-site APP cleaving enzyme 1 (Bace1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu18972	NM_011792.6 Latest version!	Mus musculus beta-site APP cleaving enzyme 1 (Bace1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$293.30 $419.00
View Old Accession Versions >>
OMu69121	XM_011242513.2	Mus musculus beta-site APP cleaving enzyme 1 (Bace1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu69120	XM_011242512.2	Mus musculus beta-site APP cleaving enzyme 1 (Bace1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu18972	NM_011792.5	Mus musculus beta-site APP cleaving enzyme 1 (Bace1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$293.30 $419.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BACE1 ( XM_011242513.2 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_011240815.1 Mus musculus beta-site APP cleaving enzyme 1 (Bace1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu69121
Clone ID Related Accession (Same CDS sequence)	XM_011242513.3 , XM_011242513.2
Accession Version	XM_011242513.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 804bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	beta-secretase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011242513.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCATTG GTGGTATCGA CCACTCGCTA TACACGGGCA GTCTCTGGTA CACACCCATC 
CGGCGGGAGT GGTATTATGA AGTGATCATT GTACGTGTGG AAATCAATGG TCAAGATCTC 
AAGATGGACT GCAAGGAGTA CAACTACGAC AAGAGCATTG TGGACAGTGG GACCACCAAC 
CTTCGCTTGC CCAAGAAAGT ATTTGAAGCT GCCGTCAAGT CCATCAAGGC AGCCTCCTCG 
ACGGAGAAGT TCCCGGATGG CTTTTGGCTA GGGGAGCAGC TGGTGTGCTG GCAAGCAGGC 
ACGACCCCTT GGAACATTTT CCCAGTCATT TCACTTTACC TCATGGGTGA AGTCACCAAT 
CAGTCCTTCC GCATCACCAT CCTTCCTCAG CAATACCTAC GGCCGGTGGA GGACGTGGCC 
ACGTCCCAAG ACGACTGTTA CAAGTTCGCT GTCTCACAGT CATCCACGGG CACTGTTATG 
GGAGCCGTCA TCATGGAAGG TTTCTATGTC GTCTTCGATC GAGCCCGAAA GCGAATTGGC 
TTTGCTGTCA GCGCTTGCCA TGTGCACGAT GAGTTCAGGA CGGCGGCAGT GGAAGGTCCG 
TTTGTTACGG CAGACATGGA AGACTGTGGC TACAACATTC CCCAGACAGA TGAGTCAACA 
CTTATGACCA TAGCCTATGT CATGGCGGCC ATCTGCGCCC TCTTCATGTT GCCACTCTGC 
CTCATGGTAT GTCAGTGGCG CTGCCTGCGT TGCCTGCGCC ACCAGCACGA TGACTTTGCT 
GATGACATCT CCCTGCTCAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011240815.1
CDS	303..1106
Translation	MIIGGIDHSLYTGSLWYTPIRREWYYEVIIVRVEINGQDLKMDCKEYNYDKSIVDSGTTNLRLPKKVFEAAVKSIKAASSTEKFPDGFWLGEQLVCWQAGTTPWNIFPVISLYLMGEVTNQSFRITILPQQYLRPVEDVATSQDDCYKFAVSQSSTGTVMGAVIMEGFYVVFDRARKRIGFAVSACHVHDEFRTAAVEGPFVTADMEDCGYNIPQTDESTLMTIAYVMAAICALFMLPLCLMVCQWRCLRCLRHQHDDFADDISLLK

Target ORF information:

RefSeq Version	XM_011242513.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus beta-site APP cleaving enzyme 1 (Bace1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011242513.2

ORF Insert Sequence:

ATGATCATTG GTGGTATCGA CCACTCGCTA TACACGGGCA GTCTCTGGTA CACACCCATC 
CGGCGGGAGT GGTATTATGA AGTGATCATT GTACGTGTGG AAATCAATGG TCAAGATCTC 
AAGATGGACT GCAAGGAGTA CAACTACGAC AAGAGCATTG TGGACAGTGG GACCACCAAC 
CTTCGCTTGC CCAAGAAAGT ATTTGAAGCT GCCGTCAAGT CCATCAAGGC AGCCTCCTCG 
ACGGAGAAGT TCCCGGATGG CTTTTGGCTA GGGGAGCAGC TGGTGTGCTG GCAAGCAGGC 
ACGACCCCTT GGAACATTTT CCCAGTCATT TCACTTTACC TCATGGGTGA AGTCACCAAT 
CAGTCCTTCC GCATCACCAT CCTTCCTCAG CAATACCTAC GGCCGGTGGA GGACGTGGCC 
ACGTCCCAAG ACGACTGTTA CAAGTTCGCT GTCTCACAGT CATCCACGGG CACTGTTATG 
GGAGCCGTCA TCATGGAAGG TTTCTATGTC GTCTTCGATC GAGCCCGAAA GCGAATTGGC 
TTTGCTGTCA GCGCTTGCCA TGTGCACGAT GAGTTCAGGA CGGCGGCAGT GGAAGGTCCG 
TTTGTTACGG CAGACATGGA AGACTGTGGC TACAACATTC CCCAGACAGA TGAGTCAACA 
CTTATGACCA TAGCCTATGT CATGGCGGCC ATCTGCGCCC TCTTCATGTT GCCACTCTGC 
CTCATGGTAT GTCAGTGGCG CTGCCTGCGT TGCCTGCGCC ACCAGCACGA TGACTTTGCT 
GATGACATCT CCCTGCTCAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BACE1 ( NM_001145947.2 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_001139419.1 Mus musculus beta-site APP cleaving enzyme 1 (Bace1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu18995
Clone ID Related Accession (Same CDS sequence)	NM_001145947.2
Accession Version	NM_001145947.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1404bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	beta-secretase 1 isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC126804.3, AK082317.1, AK033112.1 and BC048189.1. On Jan 8, 2016 this sequence version replaced NM_001145947.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' coding region compared to variant 1. This results in a shorter protein (isoform 2) compared to isoform 1. It is not known whether this isoform (2) is proteolytically processed in the same manner as isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK082317.1, SRR1660821.183606.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849382 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## regulatory uORF :: PMID: 15034149 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCCCAG CGCTGCACTG GCTCCTGCTA TGGGTGGGCT CGGGAATGCT GCCTGCCCAG 
GGAACCCATC TCGGCATCCG GCTGCCCCTT CGCAGCGGCC TGGCAGGGCC ACCCCTGGGC 
CTGAGGCTGC CCCGGGAGAC CGACGAGGAA TCGGAGGAGC CTGGCCGGAG AGGCAGCTTT 
GTGGAGATGG TGGACAACCT GAGGGGAAAG TCCGGCCAGG GCTACTATGT GGAGATGACC 
GTAGGCAGCC CCCCACAGAC GCTCAACATC CTGGTGGACA CGGGCAGTAG TAACTTTGCA 
GTGGGGGCTG CCCCACACCC TTTCCTGCAT CGCTACTACC AGAGGCAGCT GTCCAGCACA 
TATCGAGACC TCCGAAAGGG TGTGTATGTG CCCTACACCC AGGGCAAGTG GGAGGGGGAA 
CTGGGCACCG ACCTGGTGAG CATCCCTCAT GGCCCCAACG TCACTGTGCG TGCCAACATT 
GCTGCCATCA CTGAATCGGA CAAGTTCTTC ATCAATGGTT CCAACTGGGA GGGCATCCTA 
GGGCTGGCCT ATGCTGAGAT TGCCAGGCCC GACGACTCTT TGGAGCCCTT CTTTGACTCC 
CTGGTGAAGC AGACCCACAT TCCCAACATC TTTTCCCTGC AGCTCTGTGG CGCTGGCTTC 
CCCCTCAACC AGACCGAGGC ACTGGCCTCG GTGGGAGGGA GCATGATCAT TGGTGGTATC 
GACCACTCGC TATACACGGG CAGTCTCTGG TACACACCCA TCCGGCGGGA GTGGTATTAT 
GAAGTGATCA TTGTACGTGT GGAAATCAAT GGTCAAGATC TCAAGATGGA CTGCAAGGAG 
ACGGAGAAGT TCCCGGATGG CTTTTGGCTA GGGGAGCAGC TGGTGTGCTG GCAAGCAGGC 
ACGACCCCTT GGAACATTTT CCCAGTCATT TCACTTTACC TCATGGGTGA AGTCACCAAT 
CAGTCCTTCC GCATCACCAT CCTTCCTCAG CAATACCTAC GGCCGGTGGA GGACGTGGCC 
ACGTCCCAAG ACGACTGTTA CAAGTTCGCT GTCTCACAGT CATCCACGGG CACTGTTATG 
GGAGCCGTCA TCATGGAAGG TTTCTATGTC GTCTTCGATC GAGCCCGAAA GCGAATTGGC 
TTTGCTGTCA GCGCTTGCCA TGTGCACGAT GAGTTCAGGA CGGCGGCAGT GGAAGGTCCG 
TTTGTTACGG CAGACATGGA AGACTGTGGC TACAACATTC CCCAGACAGA TGAGTCAACA 
CTTATGACCA TAGCCTATGT CATGGCGGCC ATCTGCGCCC TCTTCATGTT GCCACTCTGC 
CTCATGGTAT GTCAGTGGCG CTGCCTGCGT TGCCTGCGCC ACCAGCACGA TGACTTTGCT 
GATGACATCT CCCTGCTCAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001139419.1
CDS	484..1887
Translation	MAPALHWLLLWVGSGMLPAQGTHLGIRLPLRSGLAGPPLGLRLPRETDEESEEPGRRGSFVEMVDNLRGKSGQGYYVEMTVGSPPQTLNILVDTGSSNFAVGAAPHPFLHRYYQRQLSSTYRDLRKGVYVPYTQGKWEGELGTDLVSIPHGPNVTVRANIAAITESDKFFINGSNWEGILGLAYAEIARPDDSLEPFFDSLVKQTHIPNIFSLQLCGAGFPLNQTEALASVGGSMIIGGIDHSLYTGSLWYTPIRREWYYEVIIVRVEINGQDLKMDCKETEKFPDGFWLGEQLVCWQAGTTPWNIFPVISLYLMGEVTNQSFRITILPQQYLRPVEDVATSQDDCYKFAVSQSSTGTVMGAVIMEGFYVVFDRARKRIGFAVSACHVHDEFRTAAVEGPFVTADMEDCGYNIPQTDESTLMTIAYVMAAICALFMLPLCLMVCQWRCLRCLRHQHDDFADDISLLK

Target ORF information:

RefSeq Version	NM_001145947.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus beta-site APP cleaving enzyme 1 (Bace1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001145947.2

ORF Insert Sequence:

ATGGCCCCAG CGCTGCACTG GCTCCTGCTA TGGGTGGGCT CGGGAATGCT GCCTGCCCAG 
GGAACCCATC TCGGCATCCG GCTGCCCCTT CGCAGCGGCC TGGCAGGGCC ACCCCTGGGC 
CTGAGGCTGC CCCGGGAGAC CGACGAGGAA TCGGAGGAGC CTGGCCGGAG AGGCAGCTTT 
GTGGAGATGG TGGACAACCT GAGGGGAAAG TCCGGCCAGG GCTACTATGT GGAGATGACC 
GTAGGCAGCC CCCCACAGAC GCTCAACATC CTGGTGGACA CGGGCAGTAG TAACTTTGCA 
GTGGGGGCTG CCCCACACCC TTTCCTGCAT CGCTACTACC AGAGGCAGCT GTCCAGCACA 
TATCGAGACC TCCGAAAGGG TGTGTATGTG CCCTACACCC AGGGCAAGTG GGAGGGGGAA 
CTGGGCACCG ACCTGGTGAG CATCCCTCAT GGCCCCAACG TCACTGTGCG TGCCAACATT 
GCTGCCATCA CTGAATCGGA CAAGTTCTTC ATCAATGGTT CCAACTGGGA GGGCATCCTA 
GGGCTGGCCT ATGCTGAGAT TGCCAGGCCC GACGACTCTT TGGAGCCCTT CTTTGACTCC 
CTGGTGAAGC AGACCCACAT TCCCAACATC TTTTCCCTGC AGCTCTGTGG CGCTGGCTTC 
CCCCTCAACC AGACCGAGGC ACTGGCCTCG GTGGGAGGGA GCATGATCAT TGGTGGTATC 
GACCACTCGC TATACACGGG CAGTCTCTGG TACACACCCA TCCGGCGGGA GTGGTATTAT 
GAAGTGATCA TTGTACGTGT GGAAATCAAT GGTCAAGATC TCAAGATGGA CTGCAAGGAG 
ACGGAGAAGT TCCCGGATGG CTTTTGGCTA GGGGAGCAGC TGGTGTGCTG GCAAGCAGGC 
ACGACCCCTT GGAACATTTT CCCAGTCATT TCACTTTACC TCATGGGTGA AGTCACCAAT 
CAGTCCTTCC GCATCACCAT CCTTCCTCAG CAATACCTAC GGCCGGTGGA GGACGTGGCC 
ACGTCCCAAG ACGACTGTTA CAAGTTCGCT GTCTCACAGT CATCCACGGG CACTGTTATG 
GGAGCCGTCA TCATGGAAGG TTTCTATGTC GTCTTCGATC GAGCCCGAAA GCGAATTGGC 
TTTGCTGTCA GCGCTTGCCA TGTGCACGAT GAGTTCAGGA CGGCGGCAGT GGAAGGTCCG 
TTTGTTACGG CAGACATGGA AGACTGTGGC TACAACATTC CCCAGACAGA TGAGTCAACA 
CTTATGACCA TAGCCTATGT CATGGCGGCC ATCTGCGCCC TCTTCATGTT GCCACTCTGC 
CTCATGGTAT GTCAGTGGCG CTGCCTGCGT TGCCTGCGCC ACCAGCACGA TGACTTTGCT 
GATGACATCT CCCTGCTCAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BACE1 ( XM_011242512.2 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_011240814.1 Mus musculus beta-site APP cleaving enzyme 1 (Bace1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu69120
Clone ID Related Accession (Same CDS sequence)	XM_011242512.2 , XM_011242512.3
Accession Version	XM_011242512.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1311bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	beta-secretase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011242512.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCACAC AGCCATGGGA AGGGACTTCT CCCTGTCTTT TCCCGTCTTT TCCAACAGTT 
TCCAAGCTCA ACATCCTGGT GGACACGGGC AGTAGTAACT TTGCAGTGGG GGCTGCCCCA 
CACCCTTTCC TGCATCGCTA CTACCAGAGG CAGCTGTCCA GCACATATCG AGACCTCCGA 
AAGGGTGTGT ATGTGCCCTA CACCCAGGGC AAGTGGGAGG GGGAACTGGG CACCGACCTG 
GTGAGCATCC CTCATGGCCC CAACGTCACT GTGCGTGCCA ACATTGCTGC CATCACTGAA 
TCGGACAAGT TCTTCATCAA TGGTTCCAAC TGGGAGGGCA TCCTAGGGCT GGCCTATGCT 
GAGATTGCCA GGCCCGACGA CTCTTTGGAG CCCTTCTTTG ACTCCCTGGT GAAGCAGACC 
CACATTCCCA ACATCTTTTC CCTGCAGCTC TGTGGCGCTG GCTTCCCCCT CAACCAGACC 
GAGGCACTGG CCTCGGTGGG AGGGAGCATG ATCATTGGTG GTATCGACCA CTCGCTATAC 
ACGGGCAGTC TCTGGTACAC ACCCATCCGG CGGGAGTGGT ATTATGAAGT GATCATTGTA 
CGTGTGGAAA TCAATGGTCA AGATCTCAAG ATGGACTGCA AGGAGTACAA CTACGACAAG 
AGCATTGTGG ACAGTGGGAC CACCAACCTT CGCTTGCCCA AGAAAGTATT TGAAGCTGCC 
GTCAAGTCCA TCAAGGCAGC CTCCTCGACG GAGAAGTTCC CGGATGGCTT TTGGCTAGGG 
GAGCAGCTGG TGTGCTGGCA AGCAGGCACG ACCCCTTGGA ACATTTTCCC AGTCATTTCA 
CTTTACCTCA TGGGTGAAGT CACCAATCAG TCCTTCCGCA TCACCATCCT TCCTCAGCAA 
TACCTACGGC CGGTGGAGGA CGTGGCCACG TCCCAAGACG ACTGTTACAA GTTCGCTGTC 
TCACAGTCAT CCACGGGCAC TGTTATGGGA GCCGTCATCA TGGAAGGTTT CTATGTCGTC 
TTCGATCGAG CCCGAAAGCG AATTGGCTTT GCTGTCAGCG CTTGCCATGT GCACGATGAG 
TTCAGGACGG CGGCAGTGGA AGGTCCGTTT GTTACGGCAG ACATGGAAGA CTGTGGCTAC 
AACATTCCCC AGACAGATGA GTCAACACTT ATGACCATAG CCTATGTCAT GGCGGCCATC 
TGCGCCCTCT TCATGTTGCC ACTCTGCCTC ATGGTATGTC AGTGGCGCTG CCTGCGTTGC 
CTGCGCCACC AGCACGATGA CTTTGCTGAT GACATCTCCC TGCTCAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011240814.1
CDS	16..1326
Translation	MGTQPWEGTSPCLFPSFPTVSKLNILVDTGSSNFAVGAAPHPFLHRYYQRQLSSTYRDLRKGVYVPYTQGKWEGELGTDLVSIPHGPNVTVRANIAAITESDKFFINGSNWEGILGLAYAEIARPDDSLEPFFDSLVKQTHIPNIFSLQLCGAGFPLNQTEALASVGGSMIIGGIDHSLYTGSLWYTPIRREWYYEVIIVRVEINGQDLKMDCKEYNYDKSIVDSGTTNLRLPKKVFEAAVKSIKAASSTEKFPDGFWLGEQLVCWQAGTTPWNIFPVISLYLMGEVTNQSFRITILPQQYLRPVEDVATSQDDCYKFAVSQSSTGTVMGAVIMEGFYVVFDRARKRIGFAVSACHVHDEFRTAAVEGPFVTADMEDCGYNIPQTDESTLMTIAYVMAAICALFMLPLCLMVCQWRCLRCLRHQHDDFADDISLLK

Target ORF information:

RefSeq Version	XM_011242512.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus beta-site APP cleaving enzyme 1 (Bace1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011242512.2

ORF Insert Sequence:

ATGGGCACAC AGCCATGGGA AGGGACTTCT CCCTGTCTTT TCCCGTCTTT TCCAACAGTT 
TCCAAGCTCA ACATCCTGGT GGACACGGGC AGTAGTAACT TTGCAGTGGG GGCTGCCCCA 
CACCCTTTCC TGCATCGCTA CTACCAGAGG CAGCTGTCCA GCACATATCG AGACCTCCGA 
AAGGGTGTGT ATGTGCCCTA CACCCAGGGC AAGTGGGAGG GGGAACTGGG CACCGACCTG 
GTGAGCATCC CTCATGGCCC CAACGTCACT GTGCGTGCCA ACATTGCTGC CATCACTGAA 
TCGGACAAGT TCTTCATCAA TGGTTCCAAC TGGGAGGGCA TCCTAGGGCT GGCCTATGCT 
GAGATTGCCA GGCCCGACGA CTCTTTGGAG CCCTTCTTTG ACTCCCTGGT GAAGCAGACC 
CACATTCCCA ACATCTTTTC CCTGCAGCTC TGTGGCGCTG GCTTCCCCCT CAACCAGACC 
GAGGCACTGG CCTCGGTGGG AGGGAGCATG ATCATTGGTG GTATCGACCA CTCGCTATAC 
ACGGGCAGTC TCTGGTACAC ACCCATCCGG CGGGAGTGGT ATTATGAAGT GATCATTGTA 
CGTGTGGAAA TCAATGGTCA AGATCTCAAG ATGGACTGCA AGGAGTACAA CTACGACAAG 
AGCATTGTGG ACAGTGGGAC CACCAACCTT CGCTTGCCCA AGAAAGTATT TGAAGCTGCC 
GTCAAGTCCA TCAAGGCAGC CTCCTCGACG GAGAAGTTCC CGGATGGCTT TTGGCTAGGG 
GAGCAGCTGG TGTGCTGGCA AGCAGGCACG ACCCCTTGGA ACATTTTCCC AGTCATTTCA 
CTTTACCTCA TGGGTGAAGT CACCAATCAG TCCTTCCGCA TCACCATCCT TCCTCAGCAA 
TACCTACGGC CGGTGGAGGA CGTGGCCACG TCCCAAGACG ACTGTTACAA GTTCGCTGTC 
TCACAGTCAT CCACGGGCAC TGTTATGGGA GCCGTCATCA TGGAAGGTTT CTATGTCGTC 
TTCGATCGAG CCCGAAAGCG AATTGGCTTT GCTGTCAGCG CTTGCCATGT GCACGATGAG 
TTCAGGACGG CGGCAGTGGA AGGTCCGTTT GTTACGGCAG ACATGGAAGA CTGTGGCTAC 
AACATTCCCC AGACAGATGA GTCAACACTT ATGACCATAG CCTATGTCAT GGCGGCCATC 
TGCGCCCTCT TCATGTTGCC ACTCTGCCTC ATGGTATGTC AGTGGCGCTG CCTGCGTTGC 
CTGCGCCACC AGCACGATGA CTTTGCTGAT GACATCTCCC TGCTCAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BACE1 ( XM_011242512.3 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_011240814.1 Mus musculus beta-site APP cleaving enzyme 1 (Bace1), transcript variant X1, mRNA.

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CloneID	OMu69120
Clone ID Related Accession (Same CDS sequence)	XM_011242512.2 , XM_011242512.3
Accession Version	XM_011242512.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1311bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	beta-secretase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011242512.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCACAC AGCCATGGGA AGGGACTTCT CCCTGTCTTT TCCCGTCTTT TCCAACAGTT 
TCCAAGCTCA ACATCCTGGT GGACACGGGC AGTAGTAACT TTGCAGTGGG GGCTGCCCCA 
CACCCTTTCC TGCATCGCTA CTACCAGAGG CAGCTGTCCA GCACATATCG AGACCTCCGA 
AAGGGTGTGT ATGTGCCCTA CACCCAGGGC AAGTGGGAGG GGGAACTGGG CACCGACCTG 
GTGAGCATCC CTCATGGCCC CAACGTCACT GTGCGTGCCA ACATTGCTGC CATCACTGAA 
TCGGACAAGT TCTTCATCAA TGGTTCCAAC TGGGAGGGCA TCCTAGGGCT GGCCTATGCT 
GAGATTGCCA GGCCCGACGA CTCTTTGGAG CCCTTCTTTG ACTCCCTGGT GAAGCAGACC 
CACATTCCCA ACATCTTTTC CCTGCAGCTC TGTGGCGCTG GCTTCCCCCT CAACCAGACC 
GAGGCACTGG CCTCGGTGGG AGGGAGCATG ATCATTGGTG GTATCGACCA CTCGCTATAC 
ACGGGCAGTC TCTGGTACAC ACCCATCCGG CGGGAGTGGT ATTATGAAGT GATCATTGTA 
CGTGTGGAAA TCAATGGTCA AGATCTCAAG ATGGACTGCA AGGAGTACAA CTACGACAAG 
AGCATTGTGG ACAGTGGGAC CACCAACCTT CGCTTGCCCA AGAAAGTATT TGAAGCTGCC 
GTCAAGTCCA TCAAGGCAGC CTCCTCGACG GAGAAGTTCC CGGATGGCTT TTGGCTAGGG 
GAGCAGCTGG TGTGCTGGCA AGCAGGCACG ACCCCTTGGA ACATTTTCCC AGTCATTTCA 
CTTTACCTCA TGGGTGAAGT CACCAATCAG TCCTTCCGCA TCACCATCCT TCCTCAGCAA 
TACCTACGGC CGGTGGAGGA CGTGGCCACG TCCCAAGACG ACTGTTACAA GTTCGCTGTC 
TCACAGTCAT CCACGGGCAC TGTTATGGGA GCCGTCATCA TGGAAGGTTT CTATGTCGTC 
TTCGATCGAG CCCGAAAGCG AATTGGCTTT GCTGTCAGCG CTTGCCATGT GCACGATGAG 
TTCAGGACGG CGGCAGTGGA AGGTCCGTTT GTTACGGCAG ACATGGAAGA CTGTGGCTAC 
AACATTCCCC AGACAGATGA GTCAACACTT ATGACCATAG CCTATGTCAT GGCGGCCATC 
TGCGCCCTCT TCATGTTGCC ACTCTGCCTC ATGGTATGTC AGTGGCGCTG CCTGCGTTGC 
CTGCGCCACC AGCACGATGA CTTTGCTGAT GACATCTCCC TGCTCAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011240814.1
CDS	259..1569
Translation	MGTQPWEGTSPCLFPSFPTVSKLNILVDTGSSNFAVGAAPHPFLHRYYQRQLSSTYRDLRKGVYVPYTQGKWEGELGTDLVSIPHGPNVTVRANIAAITESDKFFINGSNWEGILGLAYAEIARPDDSLEPFFDSLVKQTHIPNIFSLQLCGAGFPLNQTEALASVGGSMIIGGIDHSLYTGSLWYTPIRREWYYEVIIVRVEINGQDLKMDCKEYNYDKSIVDSGTTNLRLPKKVFEAAVKSIKAASSTEKFPDGFWLGEQLVCWQAGTTPWNIFPVISLYLMGEVTNQSFRITILPQQYLRPVEDVATSQDDCYKFAVSQSSTGTVMGAVIMEGFYVVFDRARKRIGFAVSACHVHDEFRTAAVEGPFVTADMEDCGYNIPQTDESTLMTIAYVMAAICALFMLPLCLMVCQWRCLRCLRHQHDDFADDISLLK

Target ORF information:

RefSeq Version	XM_011242512.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus beta-site APP cleaving enzyme 1 (Bace1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011242512.3

ORF Insert Sequence:

ATGGGCACAC AGCCATGGGA AGGGACTTCT CCCTGTCTTT TCCCGTCTTT TCCAACAGTT 
TCCAAGCTCA ACATCCTGGT GGACACGGGC AGTAGTAACT TTGCAGTGGG GGCTGCCCCA 
CACCCTTTCC TGCATCGCTA CTACCAGAGG CAGCTGTCCA GCACATATCG AGACCTCCGA 
AAGGGTGTGT ATGTGCCCTA CACCCAGGGC AAGTGGGAGG GGGAACTGGG CACCGACCTG 
GTGAGCATCC CTCATGGCCC CAACGTCACT GTGCGTGCCA ACATTGCTGC CATCACTGAA 
TCGGACAAGT TCTTCATCAA TGGTTCCAAC TGGGAGGGCA TCCTAGGGCT GGCCTATGCT 
GAGATTGCCA GGCCCGACGA CTCTTTGGAG CCCTTCTTTG ACTCCCTGGT GAAGCAGACC 
CACATTCCCA ACATCTTTTC CCTGCAGCTC TGTGGCGCTG GCTTCCCCCT CAACCAGACC 
GAGGCACTGG CCTCGGTGGG AGGGAGCATG ATCATTGGTG GTATCGACCA CTCGCTATAC 
ACGGGCAGTC TCTGGTACAC ACCCATCCGG CGGGAGTGGT ATTATGAAGT GATCATTGTA 
CGTGTGGAAA TCAATGGTCA AGATCTCAAG ATGGACTGCA AGGAGTACAA CTACGACAAG 
AGCATTGTGG ACAGTGGGAC CACCAACCTT CGCTTGCCCA AGAAAGTATT TGAAGCTGCC 
GTCAAGTCCA TCAAGGCAGC CTCCTCGACG GAGAAGTTCC CGGATGGCTT TTGGCTAGGG 
GAGCAGCTGG TGTGCTGGCA AGCAGGCACG ACCCCTTGGA ACATTTTCCC AGTCATTTCA 
CTTTACCTCA TGGGTGAAGT CACCAATCAG TCCTTCCGCA TCACCATCCT TCCTCAGCAA 
TACCTACGGC CGGTGGAGGA CGTGGCCACG TCCCAAGACG ACTGTTACAA GTTCGCTGTC 
TCACAGTCAT CCACGGGCAC TGTTATGGGA GCCGTCATCA TGGAAGGTTT CTATGTCGTC 
TTCGATCGAG CCCGAAAGCG AATTGGCTTT GCTGTCAGCG CTTGCCATGT GCACGATGAG 
TTCAGGACGG CGGCAGTGGA AGGTCCGTTT GTTACGGCAG ACATGGAAGA CTGTGGCTAC 
AACATTCCCC AGACAGATGA GTCAACACTT ATGACCATAG CCTATGTCAT GGCGGCCATC 
TGCGCCCTCT TCATGTTGCC ACTCTGCCTC ATGGTATGTC AGTGGCGCTG CCTGCGTTGC 
CTGCGCCACC AGCACGATGA CTTTGCTGAT GACATCTCCC TGCTCAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BACE1 ( XM_011242513.3 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_011240815.1 Mus musculus beta-site APP cleaving enzyme 1 (Bace1), transcript variant X2, mRNA.

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CloneID	OMu69121
Clone ID Related Accession (Same CDS sequence)	XM_011242513.3 , XM_011242513.2
Accession Version	XM_011242513.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 804bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	beta-secretase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011242513.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCATTG GTGGTATCGA CCACTCGCTA TACACGGGCA GTCTCTGGTA CACACCCATC 
CGGCGGGAGT GGTATTATGA AGTGATCATT GTACGTGTGG AAATCAATGG TCAAGATCTC 
AAGATGGACT GCAAGGAGTA CAACTACGAC AAGAGCATTG TGGACAGTGG GACCACCAAC 
CTTCGCTTGC CCAAGAAAGT ATTTGAAGCT GCCGTCAAGT CCATCAAGGC AGCCTCCTCG 
ACGGAGAAGT TCCCGGATGG CTTTTGGCTA GGGGAGCAGC TGGTGTGCTG GCAAGCAGGC 
ACGACCCCTT GGAACATTTT CCCAGTCATT TCACTTTACC TCATGGGTGA AGTCACCAAT 
CAGTCCTTCC GCATCACCAT CCTTCCTCAG CAATACCTAC GGCCGGTGGA GGACGTGGCC 
ACGTCCCAAG ACGACTGTTA CAAGTTCGCT GTCTCACAGT CATCCACGGG CACTGTTATG 
GGAGCCGTCA TCATGGAAGG TTTCTATGTC GTCTTCGATC GAGCCCGAAA GCGAATTGGC 
TTTGCTGTCA GCGCTTGCCA TGTGCACGAT GAGTTCAGGA CGGCGGCAGT GGAAGGTCCG 
TTTGTTACGG CAGACATGGA AGACTGTGGC TACAACATTC CCCAGACAGA TGAGTCAACA 
CTTATGACCA TAGCCTATGT CATGGCGGCC ATCTGCGCCC TCTTCATGTT GCCACTCTGC 
CTCATGGTAT GTCAGTGGCG CTGCCTGCGT TGCCTGCGCC ACCAGCACGA TGACTTTGCT 
GATGACATCT CCCTGCTCAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011240815.1
CDS	970..1773
Translation	MIIGGIDHSLYTGSLWYTPIRREWYYEVIIVRVEINGQDLKMDCKEYNYDKSIVDSGTTNLRLPKKVFEAAVKSIKAASSTEKFPDGFWLGEQLVCWQAGTTPWNIFPVISLYLMGEVTNQSFRITILPQQYLRPVEDVATSQDDCYKFAVSQSSTGTVMGAVIMEGFYVVFDRARKRIGFAVSACHVHDEFRTAAVEGPFVTADMEDCGYNIPQTDESTLMTIAYVMAAICALFMLPLCLMVCQWRCLRCLRHQHDDFADDISLLK

Target ORF information:

RefSeq Version	XM_011242513.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus beta-site APP cleaving enzyme 1 (Bace1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011242513.3

ORF Insert Sequence:

ATGATCATTG GTGGTATCGA CCACTCGCTA TACACGGGCA GTCTCTGGTA CACACCCATC 
CGGCGGGAGT GGTATTATGA AGTGATCATT GTACGTGTGG AAATCAATGG TCAAGATCTC 
AAGATGGACT GCAAGGAGTA CAACTACGAC AAGAGCATTG TGGACAGTGG GACCACCAAC 
CTTCGCTTGC CCAAGAAAGT ATTTGAAGCT GCCGTCAAGT CCATCAAGGC AGCCTCCTCG 
ACGGAGAAGT TCCCGGATGG CTTTTGGCTA GGGGAGCAGC TGGTGTGCTG GCAAGCAGGC 
ACGACCCCTT GGAACATTTT CCCAGTCATT TCACTTTACC TCATGGGTGA AGTCACCAAT 
CAGTCCTTCC GCATCACCAT CCTTCCTCAG CAATACCTAC GGCCGGTGGA GGACGTGGCC 
ACGTCCCAAG ACGACTGTTA CAAGTTCGCT GTCTCACAGT CATCCACGGG CACTGTTATG 
GGAGCCGTCA TCATGGAAGG TTTCTATGTC GTCTTCGATC GAGCCCGAAA GCGAATTGGC 
TTTGCTGTCA GCGCTTGCCA TGTGCACGAT GAGTTCAGGA CGGCGGCAGT GGAAGGTCCG 
TTTGTTACGG CAGACATGGA AGACTGTGGC TACAACATTC CCCAGACAGA TGAGTCAACA 
CTTATGACCA TAGCCTATGT CATGGCGGCC ATCTGCGCCC TCTTCATGTT GCCACTCTGC 
CTCATGGTAT GTCAGTGGCG CTGCCTGCGT TGCCTGCGCC ACCAGCACGA TGACTTTGCT 
GATGACATCT CCCTGCTCAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BACE1 ( NM_011792.6 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_035922.4 Mus musculus beta-site APP cleaving enzyme 1 (Bace1), transcript variant 1, mRNA.

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CloneID	OMu18972
Clone ID Related Accession (Same CDS sequence)	NM_011792.6 , NM_011792.5
Accession Version	NM_011792.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1506bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	beta-secretase 1 isoform 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC126804.3, AK033112.1, BC048189.1 and AK082317.1. On Jan 8, 2016 this sequence version replaced NM_011792.5. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK046175.1, AK049626.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## regulatory uORF :: PMID: 15034149 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCCCAG CGCTGCACTG GCTCCTGCTA TGGGTGGGCT CGGGAATGCT GCCTGCCCAG 
GGAACCCATC TCGGCATCCG GCTGCCCCTT CGCAGCGGCC TGGCAGGGCC ACCCCTGGGC 
CTGAGGCTGC CCCGGGAGAC CGACGAGGAA TCGGAGGAGC CTGGCCGGAG AGGCAGCTTT 
GTGGAGATGG TGGACAACCT GAGGGGAAAG TCCGGCCAGG GCTACTATGT GGAGATGACC 
GTAGGCAGCC CCCCACAGAC GCTCAACATC CTGGTGGACA CGGGCAGTAG TAACTTTGCA 
GTGGGGGCTG CCCCACACCC TTTCCTGCAT CGCTACTACC AGAGGCAGCT GTCCAGCACA 
TATCGAGACC TCCGAAAGGG TGTGTATGTG CCCTACACCC AGGGCAAGTG GGAGGGGGAA 
CTGGGCACCG ACCTGGTGAG CATCCCTCAT GGCCCCAACG TCACTGTGCG TGCCAACATT 
GCTGCCATCA CTGAATCGGA CAAGTTCTTC ATCAATGGTT CCAACTGGGA GGGCATCCTA 
GGGCTGGCCT ATGCTGAGAT TGCCAGGCCC GACGACTCTT TGGAGCCCTT CTTTGACTCC 
CTGGTGAAGC AGACCCACAT TCCCAACATC TTTTCCCTGC AGCTCTGTGG CGCTGGCTTC 
CCCCTCAACC AGACCGAGGC ACTGGCCTCG GTGGGAGGGA GCATGATCAT TGGTGGTATC 
GACCACTCGC TATACACGGG CAGTCTCTGG TACACACCCA TCCGGCGGGA GTGGTATTAT 
GAAGTGATCA TTGTACGTGT GGAAATCAAT GGTCAAGATC TCAAGATGGA CTGCAAGGAG 
TACAACTACG ACAAGAGCAT TGTGGACAGT GGGACCACCA ACCTTCGCTT GCCCAAGAAA 
GTATTTGAAG CTGCCGTCAA GTCCATCAAG GCAGCCTCCT CGACGGAGAA GTTCCCGGAT 
GGCTTTTGGC TAGGGGAGCA GCTGGTGTGC TGGCAAGCAG GCACGACCCC TTGGAACATT 
TTCCCAGTCA TTTCACTTTA CCTCATGGGT GAAGTCACCA ATCAGTCCTT CCGCATCACC 
ATCCTTCCTC AGCAATACCT ACGGCCGGTG GAGGACGTGG CCACGTCCCA AGACGACTGT 
TACAAGTTCG CTGTCTCACA GTCATCCACG GGCACTGTTA TGGGAGCCGT CATCATGGAA 
GGTTTCTATG TCGTCTTCGA TCGAGCCCGA AAGCGAATTG GCTTTGCTGT CAGCGCTTGC 
CATGTGCACG ATGAGTTCAG GACGGCGGCA GTGGAAGGTC CGTTTGTTAC GGCAGACATG 
GAAGACTGTG GCTACAACAT TCCCCAGACA GATGAGTCAA CACTTATGAC CATAGCCTAT 
GTCATGGCGG CCATCTGCGC CCTCTTCATG TTGCCACTCT GCCTCATGGT ATGTCAGTGG 
CGCTGCCTGC GTTGCCTGCG CCACCAGCAC GATGACTTTG CTGATGACAT CTCCCTGCTC 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035922.4
CDS	484..1989
Translation	MAPALHWLLLWVGSGMLPAQGTHLGIRLPLRSGLAGPPLGLRLPRETDEESEEPGRRGSFVEMVDNLRGKSGQGYYVEMTVGSPPQTLNILVDTGSSNFAVGAAPHPFLHRYYQRQLSSTYRDLRKGVYVPYTQGKWEGELGTDLVSIPHGPNVTVRANIAAITESDKFFINGSNWEGILGLAYAEIARPDDSLEPFFDSLVKQTHIPNIFSLQLCGAGFPLNQTEALASVGGSMIIGGIDHSLYTGSLWYTPIRREWYYEVIIVRVEINGQDLKMDCKEYNYDKSIVDSGTTNLRLPKKVFEAAVKSIKAASSTEKFPDGFWLGEQLVCWQAGTTPWNIFPVISLYLMGEVTNQSFRITILPQQYLRPVEDVATSQDDCYKFAVSQSSTGTVMGAVIMEGFYVVFDRARKRIGFAVSACHVHDEFRTAAVEGPFVTADMEDCGYNIPQTDESTLMTIAYVMAAICALFMLPLCLMVCQWRCLRCLRHQHDDFADDISLLK

Target ORF information:

RefSeq Version	NM_011792.6
Organism	Mus musculus(house mouse)
Definition	Mus musculus beta-site APP cleaving enzyme 1 (Bace1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011792.6

ORF Insert Sequence:

ATGGCCCCAG CGCTGCACTG GCTCCTGCTA TGGGTGGGCT CGGGAATGCT GCCTGCCCAG 
GGAACCCATC TCGGCATCCG GCTGCCCCTT CGCAGCGGCC TGGCAGGGCC ACCCCTGGGC 
CTGAGGCTGC CCCGGGAGAC CGACGAGGAA TCGGAGGAGC CTGGCCGGAG AGGCAGCTTT 
GTGGAGATGG TGGACAACCT GAGGGGAAAG TCCGGCCAGG GCTACTATGT GGAGATGACC 
GTAGGCAGCC CCCCACAGAC GCTCAACATC CTGGTGGACA CGGGCAGTAG TAACTTTGCA 
GTGGGGGCTG CCCCACACCC TTTCCTGCAT CGCTACTACC AGAGGCAGCT GTCCAGCACA 
TATCGAGACC TCCGAAAGGG TGTGTATGTG CCCTACACCC AGGGCAAGTG GGAGGGGGAA 
CTGGGCACCG ACCTGGTGAG CATCCCTCAT GGCCCCAACG TCACTGTGCG TGCCAACATT 
GCTGCCATCA CTGAATCGGA CAAGTTCTTC ATCAATGGTT CCAACTGGGA GGGCATCCTA 
GGGCTGGCCT ATGCTGAGAT TGCCAGGCCC GACGACTCTT TGGAGCCCTT CTTTGACTCC 
CTGGTGAAGC AGACCCACAT TCCCAACATC TTTTCCCTGC AGCTCTGTGG CGCTGGCTTC 
CCCCTCAACC AGACCGAGGC ACTGGCCTCG GTGGGAGGGA GCATGATCAT TGGTGGTATC 
GACCACTCGC TATACACGGG CAGTCTCTGG TACACACCCA TCCGGCGGGA GTGGTATTAT 
GAAGTGATCA TTGTACGTGT GGAAATCAAT GGTCAAGATC TCAAGATGGA CTGCAAGGAG 
TACAACTACG ACAAGAGCAT TGTGGACAGT GGGACCACCA ACCTTCGCTT GCCCAAGAAA 
GTATTTGAAG CTGCCGTCAA GTCCATCAAG GCAGCCTCCT CGACGGAGAA GTTCCCGGAT 
GGCTTTTGGC TAGGGGAGCA GCTGGTGTGC TGGCAAGCAG GCACGACCCC TTGGAACATT 
TTCCCAGTCA TTTCACTTTA CCTCATGGGT GAAGTCACCA ATCAGTCCTT CCGCATCACC 
ATCCTTCCTC AGCAATACCT ACGGCCGGTG GAGGACGTGG CCACGTCCCA AGACGACTGT 
TACAAGTTCG CTGTCTCACA GTCATCCACG GGCACTGTTA TGGGAGCCGT CATCATGGAA 
GGTTTCTATG TCGTCTTCGA TCGAGCCCGA AAGCGAATTG GCTTTGCTGT CAGCGCTTGC 
CATGTGCACG ATGAGTTCAG GACGGCGGCA GTGGAAGGTC CGTTTGTTAC GGCAGACATG 
GAAGACTGTG GCTACAACAT TCCCCAGACA GATGAGTCAA CACTTATGAC CATAGCCTAT 
GTCATGGCGG CCATCTGCGC CCTCTTCATG TTGCCACTCT GCCTCATGGT ATGTCAGTGG 
CGCTGCCTGC GTTGCCTGCG CCACCAGCAC GATGACTTTG CTGATGACAT CTCCCTGCTC 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BACE1 ( NM_011792.5 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_035922.4 Mus musculus beta-site APP cleaving enzyme 1 (Bace1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu18972
Clone ID Related Accession (Same CDS sequence)	NM_011792.6 , NM_011792.5
Accession Version	NM_011792.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1506bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-14
Organism	Mus musculus(House mouse)
Product	beta-secretase 1 isoform 1 precursor
Comment

ATGGCCCCAG CGCTGCACTG GCTCCTGCTA TGGGTGGGCT CGGGAATGCT GCCTGCCCAG 
GGAACCCATC TCGGCATCCG GCTGCCCCTT CGCAGCGGCC TGGCAGGGCC ACCCCTGGGC 
CTGAGGCTGC CCCGGGAGAC CGACGAGGAA TCGGAGGAGC CTGGCCGGAG AGGCAGCTTT 
GTGGAGATGG TGGACAACCT GAGGGGAAAG TCCGGCCAGG GCTACTATGT GGAGATGACC 
GTAGGCAGCC CCCCACAGAC GCTCAACATC CTGGTGGACA CGGGCAGTAG TAACTTTGCA 
GTGGGGGCTG CCCCACACCC TTTCCTGCAT CGCTACTACC AGAGGCAGCT GTCCAGCACA 
TATCGAGACC TCCGAAAGGG TGTGTATGTG CCCTACACCC AGGGCAAGTG GGAGGGGGAA 
CTGGGCACCG ACCTGGTGAG CATCCCTCAT GGCCCCAACG TCACTGTGCG TGCCAACATT 
GCTGCCATCA CTGAATCGGA CAAGTTCTTC ATCAATGGTT CCAACTGGGA GGGCATCCTA 
GGGCTGGCCT ATGCTGAGAT TGCCAGGCCC GACGACTCTT TGGAGCCCTT CTTTGACTCC 
CTGGTGAAGC AGACCCACAT TCCCAACATC TTTTCCCTGC AGCTCTGTGG CGCTGGCTTC 
CCCCTCAACC AGACCGAGGC ACTGGCCTCG GTGGGAGGGA GCATGATCAT TGGTGGTATC 
GACCACTCGC TATACACGGG CAGTCTCTGG TACACACCCA TCCGGCGGGA GTGGTATTAT 
GAAGTGATCA TTGTACGTGT GGAAATCAAT GGTCAAGATC TCAAGATGGA CTGCAAGGAG 
TACAACTACG ACAAGAGCAT TGTGGACAGT GGGACCACCA ACCTTCGCTT GCCCAAGAAA 
GTATTTGAAG CTGCCGTCAA GTCCATCAAG GCAGCCTCCT CGACGGAGAA GTTCCCGGAT 
GGCTTTTGGC TAGGGGAGCA GCTGGTGTGC TGGCAAGCAG GCACGACCCC TTGGAACATT 
TTCCCAGTCA TTTCACTTTA CCTCATGGGT GAAGTCACCA ATCAGTCCTT CCGCATCACC 
ATCCTTCCTC AGCAATACCT ACGGCCGGTG GAGGACGTGG CCACGTCCCA AGACGACTGT 
TACAAGTTCG CTGTCTCACA GTCATCCACG GGCACTGTTA TGGGAGCCGT CATCATGGAA 
GGTTTCTATG TCGTCTTCGA TCGAGCCCGA AAGCGAATTG GCTTTGCTGT CAGCGCTTGC 
CATGTGCACG ATGAGTTCAG GACGGCGGCA GTGGAAGGTC CGTTTGTTAC GGCAGACATG 
GAAGACTGTG GCTACAACAT TCCCCAGACA GATGAGTCAA CACTTATGAC CATAGCCTAT 
GTCATGGCGG CCATCTGCGC CCTCTTCATG TTGCCACTCT GCCTCATGGT ATGTCAGTGG 
CGCTGCCTGC GTTGCCTGCG CCACCAGCAC GATGACTTTG CTGATGACAT CTCCCTGCTC 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_035922.4

Translation

MAPALHWLLLWVGSGMLPAQGTHLGIRLPLRSGLAGPPLGLRLPRETDEESEEPGRRGSFVEMVDNLRGKSGQGYYVEMTVGSPPQTLNILVDTGSSNFAVGAAPHPFLHRYYQRQLSSTYRDLRKGVYVPYTQGKWEGELGTDLVSIPHGPNVTVRANIAAITESDKFFINGSNWEGILGLAYAEIARPDDSLEPFFDSLVKQTHIPNIFSLQLCGAGFPLNQTEALASVGGSMIIGGIDHSLYTGSLWYTPIRREWYYEVIIVRVEINGQDLKMDCKEYNYDKSIVDSGTTNLRLPKKVFEAAVKSIKAASSTEKFPDGFWLGEQLVCWQAGTTPWNIFPVISLYLMGEVTNQSFRITILPQQYLRPVEDVATSQDDCYKFAVSQSSTGTVMGAVIMEGFYVVFDRARKRIGFAVSACHVHDEFRTAAVEGPFVTADMEDCGYNIPQTDESTLMTIAYVMAAICALFMLPLCLMVCQWRCLRCLRHQHDDFADDISLLK

Target ORF information:

RefSeq Version	NM_011792.5
Organism	Mus musculus(House mouse)
Definition	Mus musculus beta-site APP cleaving enzyme 1 (Bace1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011792.5

ORF Insert Sequence:

ATGGCCCCAG CGCTGCACTG GCTCCTGCTA TGGGTGGGCT CGGGAATGCT GCCTGCCCAG 
GGAACCCATC TCGGCATCCG GCTGCCCCTT CGCAGCGGCC TGGCAGGGCC ACCCCTGGGC 
CTGAGGCTGC CCCGGGAGAC CGACGAGGAA TCGGAGGAGC CTGGCCGGAG AGGCAGCTTT 
GTGGAGATGG TGGACAACCT GAGGGGAAAG TCCGGCCAGG GCTACTATGT GGAGATGACC 
GTAGGCAGCC CCCCACAGAC GCTCAACATC CTGGTGGACA CGGGCAGTAG TAACTTTGCA 
GTGGGGGCTG CCCCACACCC TTTCCTGCAT CGCTACTACC AGAGGCAGCT GTCCAGCACA 
TATCGAGACC TCCGAAAGGG TGTGTATGTG CCCTACACCC AGGGCAAGTG GGAGGGGGAA 
CTGGGCACCG ACCTGGTGAG CATCCCTCAT GGCCCCAACG TCACTGTGCG TGCCAACATT 
GCTGCCATCA CTGAATCGGA CAAGTTCTTC ATCAATGGTT CCAACTGGGA GGGCATCCTA 
GGGCTGGCCT ATGCTGAGAT TGCCAGGCCC GACGACTCTT TGGAGCCCTT CTTTGACTCC 
CTGGTGAAGC AGACCCACAT TCCCAACATC TTTTCCCTGC AGCTCTGTGG CGCTGGCTTC 
CCCCTCAACC AGACCGAGGC ACTGGCCTCG GTGGGAGGGA GCATGATCAT TGGTGGTATC 
GACCACTCGC TATACACGGG CAGTCTCTGG TACACACCCA TCCGGCGGGA GTGGTATTAT 
GAAGTGATCA TTGTACGTGT GGAAATCAAT GGTCAAGATC TCAAGATGGA CTGCAAGGAG 
TACAACTACG ACAAGAGCAT TGTGGACAGT GGGACCACCA ACCTTCGCTT GCCCAAGAAA 
GTATTTGAAG CTGCCGTCAA GTCCATCAAG GCAGCCTCCT CGACGGAGAA GTTCCCGGAT 
GGCTTTTGGC TAGGGGAGCA GCTGGTGTGC TGGCAAGCAG GCACGACCCC TTGGAACATT 
TTCCCAGTCA TTTCACTTTA CCTCATGGGT GAAGTCACCA ATCAGTCCTT CCGCATCACC 
ATCCTTCCTC AGCAATACCT ACGGCCGGTG GAGGACGTGG CCACGTCCCA AGACGACTGT 
TACAAGTTCG CTGTCTCACA GTCATCCACG GGCACTGTTA TGGGAGCCGT CATCATGGAA 
GGTTTCTATG TCGTCTTCGA TCGAGCCCGA AAGCGAATTG GCTTTGCTGT CAGCGCTTGC 
CATGTGCACG ATGAGTTCAG GACGGCGGCA GTGGAAGGTC CGTTTGTTAC GGCAGACATG 
GAAGACTGTG GCTACAACAT TCCCCAGACA GATGAGTCAA CACTTATGAC CATAGCCTAT 
GTCATGGCGG CCATCTGCGC CCTCTTCATG TTGCCACTCT GCCTCATGGT ATGTCAGTGG 
CGCTGCCTGC GTTGCCTGCG CCACCAGCAC GATGACTTTG CTGATGACAT CTCCCTGCTC 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The normal and pathologic roles of the Alzheimer's ?-secretase, BACE1.
Current Alzheimer research11(5)441-9(2014)
Kandalepas PC,Vassar R

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BACE1 cDNA ORF clone, Mus musculus(House mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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