Gdf7 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gdf7
Entrez Gene ID	238057
Full Name	growth differentiation factor 7
Synonyms	BMP12
General protein information	Preferred Names growth differentiation factor 7 Names growth/differentiation factor 7 GDF-7
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 12 Map Location: 12\|12 A1.1
Summary	This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein may play a role in the differentiation of tendon cells and spinal cord interneurons. Mice lacking a functional copy of this gene exhibit absence of some spinal dopaminergic neurons and brain defects, male sterility, and premature death. [provided by RefSeq, Sep 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_013527.1	NP_038555.1	growth/differentiation factor 7 isoform 2 preproprotein
NM_001312876.1	NP_001299805.1	growth/differentiation factor 7 isoform 1 preproprotein

Pathways from BioSystems

Homologies

	GDF7	XP_001096970.1
Gallus gallus (chicken)	GDF7	XP_003641114.2
Bos taurus (cattle)	GDF7	NP_001193030.1
Homo sapiens (human)	GDF7	NP_878248.2
Pan troglodytes (chimpanzee)	GDF7	XP_003308955.1
Mus musculus (house mouse)	Gdf7	NP_038555.1
Rattus norvegicus (Norway rat)	Gdf7	NP_001163821.1
Danio rerio (zebrafish)	gdf7	XP_694563.2
Xenopus tropicalis (tropical clawed frog)	LOC100496775	XP_002934530.1

Gene Ontology
Bibliography

Related articles in PubMed

Geminin loss causes neural tube defects through disrupted progenitor specification and neuronal differentiation.
Patterson ES, Waller LE, Kroll KL
Developmental biology393(1)44-56(2014 Sep)

Disrupted dorsal neural tube BMP signaling in the cilia mutant Arl13b hnn stems from abnormal Shh signaling.
Horner VL, Caspary T
Developmental biology355(1)43-54(2011 Jul)

BMP-12 treatment of adult mesenchymal stem cells in vitro augments tendon-like tissue formation and defect repair in vivo.
Lee JY, Zhou Z, Taub PJ, Ramcharan M, Li Y, Akinbiyi T, Maharam ER, Leong DJ, Laudier DM, Ruike T, Torina PJ, Zaidi M, Majeska RJ, Schaffler MB, Flatow EL, Sun HB
PloS one6(3)e17531(2011 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

compensation by Gdf-5 may be at least in part responsible for the absence of a strong phenotype in GDF-7 deficient mice
Title: Effect of GDF-7 deficiency on tail tendon phenotype in mice.

Choroid plexus relationships using normal and Gdf7 (Bmp12) transgenic embryos to fate map or ablate dorsal midline cells.
Title: Direct and indirect roles of CNS dorsal midline cells in choroid plexus epithelia formation.

The following Gdf7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gdf7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu58094	NM_013527.1 Latest version!	Mus musculus growth differentiation factor 7 (Gdf7), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$265.30 $379.00
OMu73901	NM_001312876.1 Latest version!	Mus musculus growth differentiation factor 7 (Gdf7), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gdf7 ( NM_013527.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_038555.1 Mus musculus growth differentiation factor 7 (Gdf7), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu58094
Clone ID Related Accession (Same CDS sequence)	NM_013527.1
Accession Version	NM_013527.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1362bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Mus musculus(house mouse)
Product	growth/differentiation factor 7 isoform 2 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC122860.5. On May 4, 2006 this sequence version replaced XM_979218.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region, compared to variant 1. It encodes isoform 2, which is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF525752.1 [ECO:0000332] ##Evidence-Data-END##

ATGGACCTGA GCGCTGCCGC CGCGCTGTGT CTCTGGCTGC TGAGCGCTTG CCGTCCCCGC 
GACGGGCTAG AAGCTGCCGC GGTGCTCCGA GCGGCGGGGG CTGGACCAGC CTGGAGCCCC 
GGGGGCGGCG GCGGCGGGCG GACCCTCGCC CGGGCTCCAG GCCCTTCAGC TCTCCAGGCC 
GCTGCGGTTC CGGGTCCCCG AGCTGTGCGC CGCGCTGCGG GTTCTGGCTT CAGGAACGGC 
TCGGTGGTGC CACACCACTT CATGATGTCG CTTTACAGGA GCCTGGCGGG GAGGGCTCCG 
GTCGCGGCAG CTTCAGGGCA CGGGCGTGTG GACACAATCA CCGGCTTCAC AGACCAAGCA 
ACTCAAGGCC AGAGCTTCCT GTTCGACGTA TCCAGCCTCT CCGAAGCCGA TGAGGTGGTG 
AATGCGGAGC TGCGCGTGCT GCGCCGGAGG TCTCCGGAAC CAGACAGGGA CAGTGCGACC 
CTCCTTCCGC GGCTGCTGCT GTCCACGTGC CCGGACGAGG CTGGCACAGC TCACCTGCTG 
CACTCCCGGG CCGCCGAGCC CCTGGGCGGC GCGCGCTGGG AAGCGTTCGA CGTGACGGAC 
GCGGTGCAGA GCCACCGCCG CTGGCCGCGA GCCTCCCGCA AGTTCTGCCT GGTGCTGCGC 
GCGGTGACGG CCTCGGAGAG CAGCCCGCTG GCCCTGAGAC GACTGGGCTT CGGCTGGCCG 
GGCGGTGGCG ACGGCGGCGG CACTGCGGCC GAGGAGCGCG CGCTGTTGGT GATCTCCTCC 
CGTACGCAAA GGAAAGAGAG TCTGTTCCGG GAGATCCGAG CCCAGGCCCG TGCTCTCCGG 
GCCGCTGCAG AGCCGCCACC GGATCCAGGA CCAGGCGCTG GGTCACGCAA AGCCAACCTG 
GGCGGTCGCA GGCGGCGGCG GACTGCGCTG GCTGGGACTC GGGGAGCGCA GGGAAGCGGT 
GGTGGCGGCG GTGGCGGTGG CGGCGGCGGC GGCGGCGGCG GCGGCGGCGG CGGCGGCGCA 
GGCAGGGGCC ACGGGCGCAG AGGCCGGAGC CGCTGCAGTC GCAAGTCACT GCACGTGGAC 
TTTAAGGAGC TGGGCTGGGA CGACTGGATC ATCGCGCCAT TAGACTACGA GGCATACCAC 
TGCGAGGGCG TTTGCGACTT TCCTCTGCGC TCGCACCTGG AGCCTACCAA CCACGCCATC 
ATTCAGACGC TGCTCAACTC CATGGCGCCC GACGCTGCGC CAGCCTCCTG CTGCGTGCCC 
GCAAGGCTCA GTCCCATCAG CATTCTCTAC ATCGATGCCG CCAACAACGT GGTCTACAAG 
CAGTACGAAG ACATGGTGGT GGAGGCCTGC GGCTGCAGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038555.1
CDS	22..1383
Translation	MDLSAAAALCLWLLSACRPRDGLEAAAVLRAAGAGPAWSPGGGGGGRTLARAPGPSALQAAAVPGPRAVRRAAGSGFRNGSVVPHHFMMSLYRSLAGRAPVAAASGHGRVDTITGFTDQATQGQSFLFDVSSLSEADEVVNAELRVLRRRSPEPDRDSATLLPRLLLSTCPDEAGTAHLLHSRAAEPLGGARWEAFDVTDAVQSHRRWPRASRKFCLVLRAVTASESSPLALRRLGFGWPGGGDGGGTAAEERALLVISSRTQRKESLFREIRAQARALRAAAEPPPDPGPGAGSRKANLGGRRRRRTALAGTRGAQGSGGGGGGGGGGGGGGGGGGGGAGRGHGRRGRSRCSRKSLHVDFKELGWDDWIIAPLDYEAYHCEGVCDFPLRSHLEPTNHAIIQTLLNSMAPDAAPASCCVPARLSPISILYIDAANNVVYKQYEDMVVEACGCR

Target ORF information:

RefSeq Version	NM_013527.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus growth differentiation factor 7 (Gdf7), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013527.1

ORF Insert Sequence:

ATGGACCTGA GCGCTGCCGC CGCGCTGTGT CTCTGGCTGC TGAGCGCTTG CCGTCCCCGC 
GACGGGCTAG AAGCTGCCGC GGTGCTCCGA GCGGCGGGGG CTGGACCAGC CTGGAGCCCC 
GGGGGCGGCG GCGGCGGGCG GACCCTCGCC CGGGCTCCAG GCCCTTCAGC TCTCCAGGCC 
GCTGCGGTTC CGGGTCCCCG AGCTGTGCGC CGCGCTGCGG GTTCTGGCTT CAGGAACGGC 
TCGGTGGTGC CACACCACTT CATGATGTCG CTTTACAGGA GCCTGGCGGG GAGGGCTCCG 
GTCGCGGCAG CTTCAGGGCA CGGGCGTGTG GACACAATCA CCGGCTTCAC AGACCAAGCA 
ACTCAAGGCC AGAGCTTCCT GTTCGACGTA TCCAGCCTCT CCGAAGCCGA TGAGGTGGTG 
AATGCGGAGC TGCGCGTGCT GCGCCGGAGG TCTCCGGAAC CAGACAGGGA CAGTGCGACC 
CTCCTTCCGC GGCTGCTGCT GTCCACGTGC CCGGACGAGG CTGGCACAGC TCACCTGCTG 
CACTCCCGGG CCGCCGAGCC CCTGGGCGGC GCGCGCTGGG AAGCGTTCGA CGTGACGGAC 
GCGGTGCAGA GCCACCGCCG CTGGCCGCGA GCCTCCCGCA AGTTCTGCCT GGTGCTGCGC 
GCGGTGACGG CCTCGGAGAG CAGCCCGCTG GCCCTGAGAC GACTGGGCTT CGGCTGGCCG 
GGCGGTGGCG ACGGCGGCGG CACTGCGGCC GAGGAGCGCG CGCTGTTGGT GATCTCCTCC 
CGTACGCAAA GGAAAGAGAG TCTGTTCCGG GAGATCCGAG CCCAGGCCCG TGCTCTCCGG 
GCCGCTGCAG AGCCGCCACC GGATCCAGGA CCAGGCGCTG GGTCACGCAA AGCCAACCTG 
GGCGGTCGCA GGCGGCGGCG GACTGCGCTG GCTGGGACTC GGGGAGCGCA GGGAAGCGGT 
GGTGGCGGCG GTGGCGGTGG CGGCGGCGGC GGCGGCGGCG GCGGCGGCGG CGGCGGCGCA 
GGCAGGGGCC ACGGGCGCAG AGGCCGGAGC CGCTGCAGTC GCAAGTCACT GCACGTGGAC 
TTTAAGGAGC TGGGCTGGGA CGACTGGATC ATCGCGCCAT TAGACTACGA GGCATACCAC 
TGCGAGGGCG TTTGCGACTT TCCTCTGCGC TCGCACCTGG AGCCTACCAA CCACGCCATC 
ATTCAGACGC TGCTCAACTC CATGGCGCCC GACGCTGCGC CAGCCTCCTG CTGCGTGCCC 
GCAAGGCTCA GTCCCATCAG CATTCTCTAC ATCGATGCCG CCAACAACGT GGTCTACAAG 
CAGTACGAAG ACATGGTGGT GGAGGCCTGC GGCTGCAGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gdf7 ( NM_001312876.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001299805.1 Mus musculus growth differentiation factor 7 (Gdf7), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu73901
Clone ID Related Accession (Same CDS sequence)	NM_001312876.1
Accession Version	NM_001312876.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1386bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	growth/differentiation factor 7 isoform 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC122860.5. On Aug 20, 2015 this sequence version replaced XM_006515079.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##

ATGGACCTGA GCGCTGCCGC CGCGCTGTGT CTCTGGCTGC TGAGCGCTTG CCGTCCCCGC 
GACGGGCTAG AAGCTGCCGC GGTGCTCCGA GCGGCGGGGG CTGGACCAGC CTGGAGCCCC 
GGGGGCGGCG GCGGCGGGCG GACCCTCGCC CGGGCTCCAG GCCCTTCAGC TCTCCAGGCC 
GCTGCGGTTC CGGGTCCCCG AGCTGTGCGC CGCGCTGCGG GTTCTGGCTT CAGGAACGGC 
TCGGTGGTGC CACACCACTT CATGATGTCG CTTTACAGGA GCCTGGCGGG GAGGGCTCCG 
GTCGCGGCAG CTTCAGGGCA CGGGCGTGTG GACACAATCA CCGGCTTCAC AGACCAAGCA 
ACTCAAGACG AAACGGCGGC GGCCGAGCCA GGCCAGAGCT TCCTGTTCGA CGTATCCAGC 
CTCTCCGAAG CCGATGAGGT GGTGAATGCG GAGCTGCGCG TGCTGCGCCG GAGGTCTCCG 
GAACCAGACA GGGACAGTGC GACCCTCCTT CCGCGGCTGC TGCTGTCCAC GTGCCCGGAC 
GAGGCTGGCA CAGCTCACCT GCTGCACTCC CGGGCCGCCG AGCCCCTGGG CGGCGCGCGC 
TGGGAAGCGT TCGACGTGAC GGACGCGGTG CAGAGCCACC GCCGCTGGCC GCGAGCCTCC 
CGCAAGTTCT GCCTGGTGCT GCGCGCGGTG ACGGCCTCGG AGAGCAGCCC GCTGGCCCTG 
AGACGACTGG GCTTCGGCTG GCCGGGCGGT GGCGACGGCG GCGGCACTGC GGCCGAGGAG 
CGCGCGCTGT TGGTGATCTC CTCCCGTACG CAAAGGAAAG AGAGTCTGTT CCGGGAGATC 
CGAGCCCAGG CCCGTGCTCT CCGGGCCGCT GCAGAGCCGC CACCGGATCC AGGACCAGGC 
GCTGGGTCAC GCAAAGCCAA CCTGGGCGGT CGCAGGCGGC GGCGGACTGC GCTGGCTGGG 
ACTCGGGGAG CGCAGGGAAG CGGTGGTGGC GGCGGTGGCG GTGGCGGCGG CGGCGGCGGC 
GGCGGCGGCG GCGGCGGCGG CGCAGGCAGG GGCCACGGGC GCAGAGGCCG GAGCCGCTGC 
AGTCGCAAGT CACTGCACGT GGACTTTAAG GAGCTGGGCT GGGACGACTG GATCATCGCG 
CCATTAGACT ACGAGGCATA CCACTGCGAG GGCGTTTGCG ACTTTCCTCT GCGCTCGCAC 
CTGGAGCCTA CCAACCACGC CATCATTCAG ACGCTGCTCA ACTCCATGGC GCCCGACGCT 
GCGCCAGCCT CCTGCTGCGT GCCCGCAAGG CTCAGTCCCA TCAGCATTCT CTACATCGAT 
GCCGCCAACA ACGTGGTCTA CAAGCAGTAC GAAGACATGG TGGTGGAGGC CTGCGGCTGC 
AGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001299805.1
CDS	22..1407
Translation	MDLSAAAALCLWLLSACRPRDGLEAAAVLRAAGAGPAWSPGGGGGGRTLARAPGPSALQAAAVPGPRAVRRAAGSGFRNGSVVPHHFMMSLYRSLAGRAPVAAASGHGRVDTITGFTDQATQDETAAAEPGQSFLFDVSSLSEADEVVNAELRVLRRRSPEPDRDSATLLPRLLLSTCPDEAGTAHLLHSRAAEPLGGARWEAFDVTDAVQSHRRWPRASRKFCLVLRAVTASESSPLALRRLGFGWPGGGDGGGTAAEERALLVISSRTQRKESLFREIRAQARALRAAAEPPPDPGPGAGSRKANLGGRRRRRTALAGTRGAQGSGGGGGGGGGGGGGGGGGGGGAGRGHGRRGRSRCSRKSLHVDFKELGWDDWIIAPLDYEAYHCEGVCDFPLRSHLEPTNHAIIQTLLNSMAPDAAPASCCVPARLSPISILYIDAANNVVYKQYEDMVVEACGCR

Target ORF information:

RefSeq Version	NM_001312876.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus growth differentiation factor 7 (Gdf7), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001312876.1

ORF Insert Sequence:

ATGGACCTGA GCGCTGCCGC CGCGCTGTGT CTCTGGCTGC TGAGCGCTTG CCGTCCCCGC 
GACGGGCTAG AAGCTGCCGC GGTGCTCCGA GCGGCGGGGG CTGGACCAGC CTGGAGCCCC 
GGGGGCGGCG GCGGCGGGCG GACCCTCGCC CGGGCTCCAG GCCCTTCAGC TCTCCAGGCC 
GCTGCGGTTC CGGGTCCCCG AGCTGTGCGC CGCGCTGCGG GTTCTGGCTT CAGGAACGGC 
TCGGTGGTGC CACACCACTT CATGATGTCG CTTTACAGGA GCCTGGCGGG GAGGGCTCCG 
GTCGCGGCAG CTTCAGGGCA CGGGCGTGTG GACACAATCA CCGGCTTCAC AGACCAAGCA 
ACTCAAGACG AAACGGCGGC GGCCGAGCCA GGCCAGAGCT TCCTGTTCGA CGTATCCAGC 
CTCTCCGAAG CCGATGAGGT GGTGAATGCG GAGCTGCGCG TGCTGCGCCG GAGGTCTCCG 
GAACCAGACA GGGACAGTGC GACCCTCCTT CCGCGGCTGC TGCTGTCCAC GTGCCCGGAC 
GAGGCTGGCA CAGCTCACCT GCTGCACTCC CGGGCCGCCG AGCCCCTGGG CGGCGCGCGC 
TGGGAAGCGT TCGACGTGAC GGACGCGGTG CAGAGCCACC GCCGCTGGCC GCGAGCCTCC 
CGCAAGTTCT GCCTGGTGCT GCGCGCGGTG ACGGCCTCGG AGAGCAGCCC GCTGGCCCTG 
AGACGACTGG GCTTCGGCTG GCCGGGCGGT GGCGACGGCG GCGGCACTGC GGCCGAGGAG 
CGCGCGCTGT TGGTGATCTC CTCCCGTACG CAAAGGAAAG AGAGTCTGTT CCGGGAGATC 
CGAGCCCAGG CCCGTGCTCT CCGGGCCGCT GCAGAGCCGC CACCGGATCC AGGACCAGGC 
GCTGGGTCAC GCAAAGCCAA CCTGGGCGGT CGCAGGCGGC GGCGGACTGC GCTGGCTGGG 
ACTCGGGGAG CGCAGGGAAG CGGTGGTGGC GGCGGTGGCG GTGGCGGCGG CGGCGGCGGC 
GGCGGCGGCG GCGGCGGCGG CGCAGGCAGG GGCCACGGGC GCAGAGGCCG GAGCCGCTGC 
AGTCGCAAGT CACTGCACGT GGACTTTAAG GAGCTGGGCT GGGACGACTG GATCATCGCG 
CCATTAGACT ACGAGGCATA CCACTGCGAG GGCGTTTGCG ACTTTCCTCT GCGCTCGCAC 
CTGGAGCCTA CCAACCACGC CATCATTCAG ACGCTGCTCA ACTCCATGGC GCCCGACGCT 
GCGCCAGCCT CCTGCTGCGT GCCCGCAAGG CTCAGTCCCA TCAGCATTCT CTACATCGAT 
GCCGCCAACA ACGTGGTCTA CAAGCAGTAC GAAGACATGG TGGTGGAGGC CTGCGGCTGC 
AGGTAG