Ppef1 cDNA ORF clone, Mus musculus(house mouse)

The following Ppef1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ppef1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu21350 NM_011147.1
Latest version!
Mus musculus protein phosphatase with EF hand calcium-binding domain 1 (Ppef1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $384.30
$549.00
OMu73866 XM_011247818.1
Latest version!
Mus musculus protein phosphatase with EF hand calcium-binding domain 1 (Ppef1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OMu73866 XM_017318480.1
Latest version!
Mus musculus protein phosphatase with EF hand calcium-binding domain 1 (Ppef1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OMu83119 XM_017318481.1
Latest version!
Mus musculus protein phosphatase with EF hand calcium-binding domain 1 (Ppef1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OMu90394 XM_030251335.1
Latest version!
Mus musculus protein phosphatase with EF hand calcium-binding domain 1 (Ppef1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu21350
    Clone ID Related Accession (Same CDS sequence) NM_011147.1
    Accession Version NM_011147.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1953bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-09
    Organism Mus musculus(house mouse)
    Product serine/threonine-protein phosphatase with EF-hands 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL974311.6 and AL732450.5. On or before Jan 27, 2007 this sequence version replaced XM_142281.7, XM_987222.1, XM_985156.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849379, SAMN00849381 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGGCTGTG GCACTTCTTC AAAAAAAGGC AACAAATCTA AGAAAGTGAT AAAAGCCGCG 
    TTAGTCATCC AAAATTGGTA CCGGCGGTAC AGAGCTCGAC TGAGGGTCAG ACAGCATTAT
    GCCCTCGCCA TCTTCCAGTC TATTGAATAT TCTGACGAGC AAGGCCAAAT GCAGCTATCC
    AGCTTCTTTT CGTTCATGTT GGAAAACTAC ACAAAGACAA ATAATGAAGA TTCAGCATTA
    GTAACTCGAA TTTTTGACAA CACCAGGCGG GAAAGCCAAA TCAAGGATAG AGATGACTTT
    TTGGGGTTGA TAGAAGTGCC TGACTCCTAT GATGGTCCTC GGCTGCAGTT TCCTCTGACG
    TTTACTGACA TCCATATACT TCTTCAGGCC TTCAAGCAAC AGCAGATACT TCATGCTCAC
    TACGTCTTAG AGGTGCTATT TGAAGCTAGG AAAGTCCTGA AGCAAATGCC GAATTTCTCT
    CATGTAAAAA CCTTTCCCGC CAAAGAGATA ACAATATGTG GTGATTTGCA TGGGAAACTG
    GATGATCTTA TGCTGATTTT CTATAAGAAT GGCCTGCCTT CAGAGAACAA TCCATACGTT
    TTCAATGGTG ACTTTGTAGA TCGAGGAAAC AATTCCATGG AGATCCTAAT GATCCTGCTT
    GTTTGTTTTC TTGTCTACCC CTCTGACCTG CACCTCAATA GAGGGAACCA TGAAGACTTT
    ATGATGAACC TGAGGTATGG CTTCACAAAA GAAATTTTAC AAAAATACAA GCTACACGGG
    AGAAAAATCT TACAAGTCTT GGAAGAAGTC TATACCTGGC TCCCAATCGG TACAATTATT
    GACAACGAAA TCCTGGTCAT ACATGGTGGG ATATCAGAAT CCACAGACTT GAATACACTC
    CACCAGTTAC AAAGAAATAA AATGAAATCT GTGTTGATGC CGCCAGTGTT GGGAAATCAA
    GAGACTGGCG AAAAGAGAAA TAAATCAGCG TCAAATTATG TTGAACCAAG GAAGGTTGAA
    CCAGACAAGA CTCCTTCTGA AGACTTGACA AAGCAAGAAT GGGAACAAAT AGTTGACATT
    CTGTGGAGTG ACCCCAGAGG CAAAAAAGGC TGTTATCCAA ACACAAGCCG AGGAGGGGGC
    TGCTATTTTG GACCAGATGT TACCTCTAAG GTTCTTAGTA AAAACCAGTT GAAGATGCTC
    ATTAGGTCTC ATGAGTGTAA ACCCGACGGG TATGAAGTCT CTCATGATGG AAAGGTCATT
    ACTGTATTTT CTGCTTCTAA TTATTATGAA GAAGGCAGCA ACCGAGGAGC TTACATCAGA
    CTAAGTTATG GTACAATGCC ACAATTTTTC CAGTACCAAG TAACTAGTAC ATCATGCCTG
    AACCCTCTTC ACCAAAGGAT GAATGCTATG GAATCCAGTG CTTTCAAGAT ACTAAAGGAA
    AAAATGATTT CACGAAAAAC TGATCTCATC AACGCTTTCG AGCTCCGTGA CCACAGTAGG
    TCAGGAAGGA TTTCATTGGC CGAGTGGGCA TTTTCTATGG AGAACATTTT GGGGCTGAAC
    TTACCATGGA GATCTCTAAG TTCACACCTG GTAACCATTG ATTCCAGCGG AAGTGTTGAC
    TACATGTCCA GCTTTGACGA TATCCGTATT GAAAAGCCTA CGAAAGATAT GAAGTCCAAT
    CTAACTGAAA CTATGTACAG ATACCGATCT GACCTGAAAA TCATATTTAA TATCATCGAC
    AGTGATCAAT CAGGTCTGAT CTCCATGGAT GAATTTCGGA CCATGTGGAA ACTTTTCAAT
    GCTCATTACA AAGCTCATAT AGATGATTCC CAAATTGATG AGCTTGCCAG CATAGTGGAC
    TTCAACAAAG ATGGCAACAT CGACTTTAAT GAGTTTTTAA AGGCTTTCTA TGTAGTACAT
    AAATATGATA AGCCAGGGAC CAGTCTTGCT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_035277.1
    CDS121..2073
    Translation

    Target ORF information:

    RefSeq Version NM_011147.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus protein phosphatase with EF hand calcium-binding domain 1 (Ppef1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_011147.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGGCTGTG GCACTTCTTC AAAAAAAGGC AACAAATCTA AGAAAGTGAT AAAAGCCGCG 
    TTAGTCATCC AAAATTGGTA CCGGCGGTAC AGAGCTCGAC TGAGGGTCAG ACAGCATTAT
    GCCCTCGCCA TCTTCCAGTC TATTGAATAT TCTGACGAGC AAGGCCAAAT GCAGCTATCC
    AGCTTCTTTT CGTTCATGTT GGAAAACTAC ACAAAGACAA ATAATGAAGA TTCAGCATTA
    GTAACTCGAA TTTTTGACAA CACCAGGCGG GAAAGCCAAA TCAAGGATAG AGATGACTTT
    TTGGGGTTGA TAGAAGTGCC TGACTCCTAT GATGGTCCTC GGCTGCAGTT TCCTCTGACG
    TTTACTGACA TCCATATACT TCTTCAGGCC TTCAAGCAAC AGCAGATACT TCATGCTCAC
    TACGTCTTAG AGGTGCTATT TGAAGCTAGG AAAGTCCTGA AGCAAATGCC GAATTTCTCT
    CATGTAAAAA CCTTTCCCGC CAAAGAGATA ACAATATGTG GTGATTTGCA TGGGAAACTG
    GATGATCTTA TGCTGATTTT CTATAAGAAT GGCCTGCCTT CAGAGAACAA TCCATACGTT
    TTCAATGGTG ACTTTGTAGA TCGAGGAAAC AATTCCATGG AGATCCTAAT GATCCTGCTT
    GTTTGTTTTC TTGTCTACCC CTCTGACCTG CACCTCAATA GAGGGAACCA TGAAGACTTT
    ATGATGAACC TGAGGTATGG CTTCACAAAA GAAATTTTAC AAAAATACAA GCTACACGGG
    AGAAAAATCT TACAAGTCTT GGAAGAAGTC TATACCTGGC TCCCAATCGG TACAATTATT
    GACAACGAAA TCCTGGTCAT ACATGGTGGG ATATCAGAAT CCACAGACTT GAATACACTC
    CACCAGTTAC AAAGAAATAA AATGAAATCT GTGTTGATGC CGCCAGTGTT GGGAAATCAA
    GAGACTGGCG AAAAGAGAAA TAAATCAGCG TCAAATTATG TTGAACCAAG GAAGGTTGAA
    CCAGACAAGA CTCCTTCTGA AGACTTGACA AAGCAAGAAT GGGAACAAAT AGTTGACATT
    CTGTGGAGTG ACCCCAGAGG CAAAAAAGGC TGTTATCCAA ACACAAGCCG AGGAGGGGGC
    TGCTATTTTG GACCAGATGT TACCTCTAAG GTTCTTAGTA AAAACCAGTT GAAGATGCTC
    ATTAGGTCTC ATGAGTGTAA ACCCGACGGG TATGAAGTCT CTCATGATGG AAAGGTCATT
    ACTGTATTTT CTGCTTCTAA TTATTATGAA GAAGGCAGCA ACCGAGGAGC TTACATCAGA
    CTAAGTTATG GTACAATGCC ACAATTTTTC CAGTACCAAG TAACTAGTAC ATCATGCCTG
    AACCCTCTTC ACCAAAGGAT GAATGCTATG GAATCCAGTG CTTTCAAGAT ACTAAAGGAA
    AAAATGATTT CACGAAAAAC TGATCTCATC AACGCTTTCG AGCTCCGTGA CCACAGTAGG
    TCAGGAAGGA TTTCATTGGC CGAGTGGGCA TTTTCTATGG AGAACATTTT GGGGCTGAAC
    TTACCATGGA GATCTCTAAG TTCACACCTG GTAACCATTG ATTCCAGCGG AAGTGTTGAC
    TACATGTCCA GCTTTGACGA TATCCGTATT GAAAAGCCTA CGAAAGATAT GAAGTCCAAT
    CTAACTGAAA CTATGTACAG ATACCGATCT GACCTGAAAA TCATATTTAA TATCATCGAC
    AGTGATCAAT CAGGTCTGAT CTCCATGGAT GAATTTCGGA CCATGTGGAA ACTTTTCAAT
    GCTCATTACA AAGCTCATAT AGATGATTCC CAAATTGATG AGCTTGCCAG CATAGTGGAC
    TTCAACAAAG ATGGCAACAT CGACTTTAAT GAGTTTTTAA AGGCTTTCTA TGTAGTACAT
    AAATATGATA AGCCAGGGAC CAGTCTTGCT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu73866
    Clone ID Related Accession (Same CDS sequence) XM_011247818.1 , XM_017318480.1
    Accession Version XM_011247818.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2031bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product serine/threonine-protein phosphatase with EF-hands 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGGCTGTG GCACTTCTTC AAAAAAAGGC AACAAATCTA AGAAAGAGAA CACAGACATA 
    ATAATGTCCA GTATCAACTT GGTGACTTCA GCATCATTCA TAACGGAACA TTCAAGGGAC
    CTAATGATAA AAGCCGCGTT AGTCATCCAA AATTGGTACC GGCGGTACAG AGCTCGACTG
    AGGGTCAGAC AGCATTATGC CCTCGCCATC TTCCAGTCTA TTGAATATTC TGACGAGCAA
    GGCCAAATGC AGCTATCCAG CTTCTTTTCG TTCATGTTGG AAAACTACAC AAAGACAAAT
    AATGAAGATT CAGCATTAGT AACTCGAATT TTTGACAACA CCAGGCGGGA AAGCCAAATC
    AAGGATAGAG ATGACTTTTT GGGGTTGATA GAAGTGCCTG ACTCCTATGA TGGTCCTCGG
    CTGCAGTTTC CTCTGACGTT TACTGACATC CATATACTTC TTCAGGCCTT CAAGCAACAG
    CAGATACTTC ATGCTCACTA CGTCTTAGAG GTGCTATTTG AAGCTAGGAA AGTCCTGAAG
    CAAATGCCGA ATTTCTCTCA TGTAAAAACC TTTCCCGCCA AAGAGATAAC AATATGTGGT
    GATTTGCATG GGAAACTGGA TGATCTTATG CTGATTTTCT ATAAGAATGG CCTGCCTTCA
    GAGAACAATC CATACGTTTT CAATGGTGAC TTTGTAGATC GAGGAAACAA TTCCATGGAG
    ATCCTAATGA TCCTGCTTGT TTGTTTTCTT GTCTACCCCT CTGACCTGCA CCTCAATAGA
    GGGAACCATG AAGACTTTAT GATGAACCTG AGGTATGGCT TCACAAAAGA AATTTTACAA
    AAATACAAGC TACACGGGAG AAAAATCTTA CAAGTCTTGG AAGAAGTCTA TACCTGGCTC
    CCAATCGGTA CAATTATTGA CAACGAAATC CTGGTCATAC ATGGTGGGAT ATCAGAATCC
    ACAGACTTGA ATACACTCCA CCAGTTACAA AGAAATAAAA TGAAATCTGT GTTGATGCCG
    CCAGTGTTGG GAAATCAAGA GACTGGCGAA AAGAGAAATA AATCAGCGTC AAATTATGTT
    GAACCAAGGA AGGTTGAACC AGACAAGACT CCTTCTGAAG ACTTGACAAA GCAAGAATGG
    GAACAAATAG TTGACATTCT GTGGAGTGAC CCCAGAGGCA AAAAAGGCTG TTATCCAAAC
    ACAAGCCGAG GAGGGGGCTG CTATTTTGGA CCAGATGTTA CCTCTAAGGT TCTTAGTAAA
    AACCAGTTGA AGATGCTCAT TAGGTCTCAT GAGTGTAAAC CCGACGGGTA TGAAGTCTCT
    CATGATGGAA AGGTCATTAC TGTATTTTCT GCTTCTAATT ATTATGAAGA AGGCAGCAAC
    CGAGGAGCTT ACATCAGACT AAGTTATGGT ACAATGCCAC AATTTTTCCA GTACCAAGTA
    ACTAGTACAT CATGCCTGAA CCCTCTTCAC CAAAGGATGA ATGCTATGGA ATCCAGTGCT
    TTCAAGATAC TAAAGGAAAA AATGATTTCA CGAAAAACTG ATCTCATCAA CGCTTTCGAG
    CTCCGTGACC ACAGTAGGTC AGGAAGGATT TCATTGGCCG AGTGGGCATT TTCTATGGAG
    AACATTTTGG GGCTGAACTT ACCATGGAGA TCTCTAAGTT CACACCTGGT AACCATTGAT
    TCCAGCGGAA GTGTTGACTA CATGTCCAGC TTTGACGATA TCCGTATTGA AAAGCCTACG
    AAAGATATGA AGTCCAATCT AACTGAAACT ATGTACAGAT ACCGATCTGA CCTGAAAATC
    ATATTTAATA TCATCGACAG TGATCAATCA GGTCTGATCT CCATGGATGA ATTTCGGACC
    ATGTGGAAAC TTTTCAATGC TCATTACAAA GCTCATATAG ATGATTCCCA AATTGATGAG
    CTTGCCAGCA TAGTGGACTT CAACAAAGAT GGCAACATCG ACTTTAATGA GTTTTTAAAG
    GCTTTCTATG TAGTACATAA ATATGATAAG CCAGGGACCA GTCTTGCTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011246120.1
    CDS361..2391
    Translation

    Target ORF information:

    RefSeq Version XM_011247818.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus protein phosphatase with EF hand calcium-binding domain 1 (Ppef1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011247818.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGGCTGTG GCACTTCTTC AAAAAAAGGC AACAAATCTA AGAAAGAGAA CACAGACATA 
    ATAATGTCCA GTATCAACTT GGTGACTTCA GCATCATTCA TAACGGAACA TTCAAGGGAC
    CTAATGATAA AAGCCGCGTT AGTCATCCAA AATTGGTACC GGCGGTACAG AGCTCGACTG
    AGGGTCAGAC AGCATTATGC CCTCGCCATC TTCCAGTCTA TTGAATATTC TGACGAGCAA
    GGCCAAATGC AGCTATCCAG CTTCTTTTCG TTCATGTTGG AAAACTACAC AAAGACAAAT
    AATGAAGATT CAGCATTAGT AACTCGAATT TTTGACAACA CCAGGCGGGA AAGCCAAATC
    AAGGATAGAG ATGACTTTTT GGGGTTGATA GAAGTGCCTG ACTCCTATGA TGGTCCTCGG
    CTGCAGTTTC CTCTGACGTT TACTGACATC CATATACTTC TTCAGGCCTT CAAGCAACAG
    CAGATACTTC ATGCTCACTA CGTCTTAGAG GTGCTATTTG AAGCTAGGAA AGTCCTGAAG
    CAAATGCCGA ATTTCTCTCA TGTAAAAACC TTTCCCGCCA AAGAGATAAC AATATGTGGT
    GATTTGCATG GGAAACTGGA TGATCTTATG CTGATTTTCT ATAAGAATGG CCTGCCTTCA
    GAGAACAATC CATACGTTTT CAATGGTGAC TTTGTAGATC GAGGAAACAA TTCCATGGAG
    ATCCTAATGA TCCTGCTTGT TTGTTTTCTT GTCTACCCCT CTGACCTGCA CCTCAATAGA
    GGGAACCATG AAGACTTTAT GATGAACCTG AGGTATGGCT TCACAAAAGA AATTTTACAA
    AAATACAAGC TACACGGGAG AAAAATCTTA CAAGTCTTGG AAGAAGTCTA TACCTGGCTC
    CCAATCGGTA CAATTATTGA CAACGAAATC CTGGTCATAC ATGGTGGGAT ATCAGAATCC
    ACAGACTTGA ATACACTCCA CCAGTTACAA AGAAATAAAA TGAAATCTGT GTTGATGCCG
    CCAGTGTTGG GAAATCAAGA GACTGGCGAA AAGAGAAATA AATCAGCGTC AAATTATGTT
    GAACCAAGGA AGGTTGAACC AGACAAGACT CCTTCTGAAG ACTTGACAAA GCAAGAATGG
    GAACAAATAG TTGACATTCT GTGGAGTGAC CCCAGAGGCA AAAAAGGCTG TTATCCAAAC
    ACAAGCCGAG GAGGGGGCTG CTATTTTGGA CCAGATGTTA CCTCTAAGGT TCTTAGTAAA
    AACCAGTTGA AGATGCTCAT TAGGTCTCAT GAGTGTAAAC CCGACGGGTA TGAAGTCTCT
    CATGATGGAA AGGTCATTAC TGTATTTTCT GCTTCTAATT ATTATGAAGA AGGCAGCAAC
    CGAGGAGCTT ACATCAGACT AAGTTATGGT ACAATGCCAC AATTTTTCCA GTACCAAGTA
    ACTAGTACAT CATGCCTGAA CCCTCTTCAC CAAAGGATGA ATGCTATGGA ATCCAGTGCT
    TTCAAGATAC TAAAGGAAAA AATGATTTCA CGAAAAACTG ATCTCATCAA CGCTTTCGAG
    CTCCGTGACC ACAGTAGGTC AGGAAGGATT TCATTGGCCG AGTGGGCATT TTCTATGGAG
    AACATTTTGG GGCTGAACTT ACCATGGAGA TCTCTAAGTT CACACCTGGT AACCATTGAT
    TCCAGCGGAA GTGTTGACTA CATGTCCAGC TTTGACGATA TCCGTATTGA AAAGCCTACG
    AAAGATATGA AGTCCAATCT AACTGAAACT ATGTACAGAT ACCGATCTGA CCTGAAAATC
    ATATTTAATA TCATCGACAG TGATCAATCA GGTCTGATCT CCATGGATGA ATTTCGGACC
    ATGTGGAAAC TTTTCAATGC TCATTACAAA GCTCATATAG ATGATTCCCA AATTGATGAG
    CTTGCCAGCA TAGTGGACTT CAACAAAGAT GGCAACATCG ACTTTAATGA GTTTTTAAAG
    GCTTTCTATG TAGTACATAA ATATGATAAG CCAGGGACCA GTCTTGCTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu73866
    Clone ID Related Accession (Same CDS sequence) XM_011247818.1 , XM_017318480.1
    Accession Version XM_017318480.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2031bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product serine/threonine-protein phosphatase with EF-hands 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGGCTGTG GCACTTCTTC AAAAAAAGGC AACAAATCTA AGAAAGAGAA CACAGACATA 
    ATAATGTCCA GTATCAACTT GGTGACTTCA GCATCATTCA TAACGGAACA TTCAAGGGAC
    CTAATGATAA AAGCCGCGTT AGTCATCCAA AATTGGTACC GGCGGTACAG AGCTCGACTG
    AGGGTCAGAC AGCATTATGC CCTCGCCATC TTCCAGTCTA TTGAATATTC TGACGAGCAA
    GGCCAAATGC AGCTATCCAG CTTCTTTTCG TTCATGTTGG AAAACTACAC AAAGACAAAT
    AATGAAGATT CAGCATTAGT AACTCGAATT TTTGACAACA CCAGGCGGGA AAGCCAAATC
    AAGGATAGAG ATGACTTTTT GGGGTTGATA GAAGTGCCTG ACTCCTATGA TGGTCCTCGG
    CTGCAGTTTC CTCTGACGTT TACTGACATC CATATACTTC TTCAGGCCTT CAAGCAACAG
    CAGATACTTC ATGCTCACTA CGTCTTAGAG GTGCTATTTG AAGCTAGGAA AGTCCTGAAG
    CAAATGCCGA ATTTCTCTCA TGTAAAAACC TTTCCCGCCA AAGAGATAAC AATATGTGGT
    GATTTGCATG GGAAACTGGA TGATCTTATG CTGATTTTCT ATAAGAATGG CCTGCCTTCA
    GAGAACAATC CATACGTTTT CAATGGTGAC TTTGTAGATC GAGGAAACAA TTCCATGGAG
    ATCCTAATGA TCCTGCTTGT TTGTTTTCTT GTCTACCCCT CTGACCTGCA CCTCAATAGA
    GGGAACCATG AAGACTTTAT GATGAACCTG AGGTATGGCT TCACAAAAGA AATTTTACAA
    AAATACAAGC TACACGGGAG AAAAATCTTA CAAGTCTTGG AAGAAGTCTA TACCTGGCTC
    CCAATCGGTA CAATTATTGA CAACGAAATC CTGGTCATAC ATGGTGGGAT ATCAGAATCC
    ACAGACTTGA ATACACTCCA CCAGTTACAA AGAAATAAAA TGAAATCTGT GTTGATGCCG
    CCAGTGTTGG GAAATCAAGA GACTGGCGAA AAGAGAAATA AATCAGCGTC AAATTATGTT
    GAACCAAGGA AGGTTGAACC AGACAAGACT CCTTCTGAAG ACTTGACAAA GCAAGAATGG
    GAACAAATAG TTGACATTCT GTGGAGTGAC CCCAGAGGCA AAAAAGGCTG TTATCCAAAC
    ACAAGCCGAG GAGGGGGCTG CTATTTTGGA CCAGATGTTA CCTCTAAGGT TCTTAGTAAA
    AACCAGTTGA AGATGCTCAT TAGGTCTCAT GAGTGTAAAC CCGACGGGTA TGAAGTCTCT
    CATGATGGAA AGGTCATTAC TGTATTTTCT GCTTCTAATT ATTATGAAGA AGGCAGCAAC
    CGAGGAGCTT ACATCAGACT AAGTTATGGT ACAATGCCAC AATTTTTCCA GTACCAAGTA
    ACTAGTACAT CATGCCTGAA CCCTCTTCAC CAAAGGATGA ATGCTATGGA ATCCAGTGCT
    TTCAAGATAC TAAAGGAAAA AATGATTTCA CGAAAAACTG ATCTCATCAA CGCTTTCGAG
    CTCCGTGACC ACAGTAGGTC AGGAAGGATT TCATTGGCCG AGTGGGCATT TTCTATGGAG
    AACATTTTGG GGCTGAACTT ACCATGGAGA TCTCTAAGTT CACACCTGGT AACCATTGAT
    TCCAGCGGAA GTGTTGACTA CATGTCCAGC TTTGACGATA TCCGTATTGA AAAGCCTACG
    AAAGATATGA AGTCCAATCT AACTGAAACT ATGTACAGAT ACCGATCTGA CCTGAAAATC
    ATATTTAATA TCATCGACAG TGATCAATCA GGTCTGATCT CCATGGATGA ATTTCGGACC
    ATGTGGAAAC TTTTCAATGC TCATTACAAA GCTCATATAG ATGATTCCCA AATTGATGAG
    CTTGCCAGCA TAGTGGACTT CAACAAAGAT GGCAACATCG ACTTTAATGA GTTTTTAAAG
    GCTTTCTATG TAGTACATAA ATATGATAAG CCAGGGACCA GTCTTGCTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017173969.1
    CDS377..2407
    Translation

    Target ORF information:

    RefSeq Version XM_017318480.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus protein phosphatase with EF hand calcium-binding domain 1 (Ppef1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017318480.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGGCTGTG GCACTTCTTC AAAAAAAGGC AACAAATCTA AGAAAGAGAA CACAGACATA 
    ATAATGTCCA GTATCAACTT GGTGACTTCA GCATCATTCA TAACGGAACA TTCAAGGGAC
    CTAATGATAA AAGCCGCGTT AGTCATCCAA AATTGGTACC GGCGGTACAG AGCTCGACTG
    AGGGTCAGAC AGCATTATGC CCTCGCCATC TTCCAGTCTA TTGAATATTC TGACGAGCAA
    GGCCAAATGC AGCTATCCAG CTTCTTTTCG TTCATGTTGG AAAACTACAC AAAGACAAAT
    AATGAAGATT CAGCATTAGT AACTCGAATT TTTGACAACA CCAGGCGGGA AAGCCAAATC
    AAGGATAGAG ATGACTTTTT GGGGTTGATA GAAGTGCCTG ACTCCTATGA TGGTCCTCGG
    CTGCAGTTTC CTCTGACGTT TACTGACATC CATATACTTC TTCAGGCCTT CAAGCAACAG
    CAGATACTTC ATGCTCACTA CGTCTTAGAG GTGCTATTTG AAGCTAGGAA AGTCCTGAAG
    CAAATGCCGA ATTTCTCTCA TGTAAAAACC TTTCCCGCCA AAGAGATAAC AATATGTGGT
    GATTTGCATG GGAAACTGGA TGATCTTATG CTGATTTTCT ATAAGAATGG CCTGCCTTCA
    GAGAACAATC CATACGTTTT CAATGGTGAC TTTGTAGATC GAGGAAACAA TTCCATGGAG
    ATCCTAATGA TCCTGCTTGT TTGTTTTCTT GTCTACCCCT CTGACCTGCA CCTCAATAGA
    GGGAACCATG AAGACTTTAT GATGAACCTG AGGTATGGCT TCACAAAAGA AATTTTACAA
    AAATACAAGC TACACGGGAG AAAAATCTTA CAAGTCTTGG AAGAAGTCTA TACCTGGCTC
    CCAATCGGTA CAATTATTGA CAACGAAATC CTGGTCATAC ATGGTGGGAT ATCAGAATCC
    ACAGACTTGA ATACACTCCA CCAGTTACAA AGAAATAAAA TGAAATCTGT GTTGATGCCG
    CCAGTGTTGG GAAATCAAGA GACTGGCGAA AAGAGAAATA AATCAGCGTC AAATTATGTT
    GAACCAAGGA AGGTTGAACC AGACAAGACT CCTTCTGAAG ACTTGACAAA GCAAGAATGG
    GAACAAATAG TTGACATTCT GTGGAGTGAC CCCAGAGGCA AAAAAGGCTG TTATCCAAAC
    ACAAGCCGAG GAGGGGGCTG CTATTTTGGA CCAGATGTTA CCTCTAAGGT TCTTAGTAAA
    AACCAGTTGA AGATGCTCAT TAGGTCTCAT GAGTGTAAAC CCGACGGGTA TGAAGTCTCT
    CATGATGGAA AGGTCATTAC TGTATTTTCT GCTTCTAATT ATTATGAAGA AGGCAGCAAC
    CGAGGAGCTT ACATCAGACT AAGTTATGGT ACAATGCCAC AATTTTTCCA GTACCAAGTA
    ACTAGTACAT CATGCCTGAA CCCTCTTCAC CAAAGGATGA ATGCTATGGA ATCCAGTGCT
    TTCAAGATAC TAAAGGAAAA AATGATTTCA CGAAAAACTG ATCTCATCAA CGCTTTCGAG
    CTCCGTGACC ACAGTAGGTC AGGAAGGATT TCATTGGCCG AGTGGGCATT TTCTATGGAG
    AACATTTTGG GGCTGAACTT ACCATGGAGA TCTCTAAGTT CACACCTGGT AACCATTGAT
    TCCAGCGGAA GTGTTGACTA CATGTCCAGC TTTGACGATA TCCGTATTGA AAAGCCTACG
    AAAGATATGA AGTCCAATCT AACTGAAACT ATGTACAGAT ACCGATCTGA CCTGAAAATC
    ATATTTAATA TCATCGACAG TGATCAATCA GGTCTGATCT CCATGGATGA ATTTCGGACC
    ATGTGGAAAC TTTTCAATGC TCATTACAAA GCTCATATAG ATGATTCCCA AATTGATGAG
    CTTGCCAGCA TAGTGGACTT CAACAAAGAT GGCAACATCG ACTTTAATGA GTTTTTAAAG
    GCTTTCTATG TAGTACATAA ATATGATAAG CCAGGGACCA GTCTTGCTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu83119
    Clone ID Related Accession (Same CDS sequence) XM_017318481.1
    Accession Version XM_017318481.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1968bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product serine/threonine-protein phosphatase with EF-hands 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGTCCAGTA TCAACTTGGT GACTTCAGCA TCATTCATAA CGGAACATTC AAGGGACCTA 
    ATGATAAAAG CCGCGTTAGT CATCCAAAAT TGGTACCGGC GGTACAGAGC TCGACTGAGG
    GTCAGACAGC ATTATGCCCT CGCCATCTTC CAGTCTATTG AATATTCTGA CGAGCAAGGC
    CAAATGCAGC TATCCAGCTT CTTTTCGTTC ATGTTGGAAA ACTACACAAA GACAAATAAT
    GAAGATTCAG CATTAGTAAC TCGAATTTTT GACAACACCA GGCGGGAAAG CCAAATCAAG
    GATAGAGATG ACTTTTTGGG GTTGATAGAA GTGCCTGACT CCTATGATGG TCCTCGGCTG
    CAGTTTCCTC TGACGTTTAC TGACATCCAT ATACTTCTTC AGGCCTTCAA GCAACAGCAG
    ATACTTCATG CTCACTACGT CTTAGAGGTG CTATTTGAAG CTAGGAAAGT CCTGAAGCAA
    ATGCCGAATT TCTCTCATGT AAAAACCTTT CCCGCCAAAG AGATAACAAT ATGTGGTGAT
    TTGCATGGGA AACTGGATGA TCTTATGCTG ATTTTCTATA AGAATGGCCT GCCTTCAGAG
    AACAATCCAT ACGTTTTCAA TGGTGACTTT GTAGATCGAG GAAACAATTC CATGGAGATC
    CTAATGATCC TGCTTGTTTG TTTTCTTGTC TACCCCTCTG ACCTGCACCT CAATAGAGGG
    AACCATGAAG ACTTTATGAT GAACCTGAGG TATGGCTTCA CAAAAGAAAT TTTACAAAAA
    TACAAGCTAC ACGGGAGAAA AATCTTACAA GTCTTGGAAG AAGTCTATAC CTGGCTCCCA
    ATCGGTACAA TTATTGACAA CGAAATCCTG GTCATACATG GTGGGATATC AGAATCCACA
    GACTTGAATA CACTCCACCA GTTACAAAGA AATAAAATGA AATCTGTGTT GATGCCGCCA
    GTGTTGGGAA ATCAAGAGAC TGGCGAAAAG AGAAATAAAT CAGCGTCAAA TTATGTTGAA
    CCAAGGAAGG TTGAACCAGA CAAGACTCCT TCTGAAGACT TGACAAAGCA AGAATGGGAA
    CAAATAGTTG ACATTCTGTG GAGTGACCCC AGAGGCAAAA AAGGCTGTTA TCCAAACACA
    AGCCGAGGAG GGGGCTGCTA TTTTGGACCA GATGTTACCT CTAAGGTTCT TAGTAAAAAC
    CAGTTGAAGA TGCTCATTAG GTCTCATGAG TGTAAACCCG ACGGGTATGA AGTCTCTCAT
    GATGGAAAGG TCATTACTGT ATTTTCTGCT TCTAATTATT ATGAAGAAGG CAGCAACCGA
    GGAGCTTACA TCAGACTAAG TTATGGTACA ATGCCACAAT TTTTCCAGTA CCAAGTAACT
    AGTACATCAT GCCTGAACCC TCTTCACCAA AGGATGAATG CTATGGAATC CAGTGCTTTC
    AAGATACTAA AGGAAAAAAT GATTTCACGA AAAACTGATC TCATCAACGC TTTCGAGCTC
    CGTGACCACA GTAGGTCAGG AAGGATTTCA TTGGCCGAGT GGGCATTTTC TATGGAGAAC
    ATTTTGGGGC TGAACTTACC ATGGAGATCT CTAAGTTCAC ACCTGGTAAC CATTGATTCC
    AGCGGAAGTG TTGACTACAT GTCCAGCTTT GACGATATCC GTATTGAAAA GCCTACGAAA
    GATATGAAGT CCAATCTAAC TGAAACTATG TACAGATACC GATCTGACCT GAAAATCATA
    TTTAATATCA TCGACAGTGA TCAATCAGGT CTGATCTCCA TGGATGAATT TCGGACCATG
    TGGAAACTTT TCAATGCTCA TTACAAAGCT CATATAGATG ATTCCCAAAT TGATGAGCTT
    GCCAGCATAG TGGACTTCAA CAAAGATGGC AACATCGACT TTAATGAGTT TTTAAAGGCT
    TTCTATGTAG TACATAAATA TGATAAGCCA GGGACCAGTC TTGCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017173970.1
    CDS279..2246
    Translation

    Target ORF information:

    RefSeq Version XM_017318481.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus protein phosphatase with EF hand calcium-binding domain 1 (Ppef1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017318481.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGTCCAGTA TCAACTTGGT GACTTCAGCA TCATTCATAA CGGAACATTC AAGGGACCTA 
    ATGATAAAAG CCGCGTTAGT CATCCAAAAT TGGTACCGGC GGTACAGAGC TCGACTGAGG
    GTCAGACAGC ATTATGCCCT CGCCATCTTC CAGTCTATTG AATATTCTGA CGAGCAAGGC
    CAAATGCAGC TATCCAGCTT CTTTTCGTTC ATGTTGGAAA ACTACACAAA GACAAATAAT
    GAAGATTCAG CATTAGTAAC TCGAATTTTT GACAACACCA GGCGGGAAAG CCAAATCAAG
    GATAGAGATG ACTTTTTGGG GTTGATAGAA GTGCCTGACT CCTATGATGG TCCTCGGCTG
    CAGTTTCCTC TGACGTTTAC TGACATCCAT ATACTTCTTC AGGCCTTCAA GCAACAGCAG
    ATACTTCATG CTCACTACGT CTTAGAGGTG CTATTTGAAG CTAGGAAAGT CCTGAAGCAA
    ATGCCGAATT TCTCTCATGT AAAAACCTTT CCCGCCAAAG AGATAACAAT ATGTGGTGAT
    TTGCATGGGA AACTGGATGA TCTTATGCTG ATTTTCTATA AGAATGGCCT GCCTTCAGAG
    AACAATCCAT ACGTTTTCAA TGGTGACTTT GTAGATCGAG GAAACAATTC CATGGAGATC
    CTAATGATCC TGCTTGTTTG TTTTCTTGTC TACCCCTCTG ACCTGCACCT CAATAGAGGG
    AACCATGAAG ACTTTATGAT GAACCTGAGG TATGGCTTCA CAAAAGAAAT TTTACAAAAA
    TACAAGCTAC ACGGGAGAAA AATCTTACAA GTCTTGGAAG AAGTCTATAC CTGGCTCCCA
    ATCGGTACAA TTATTGACAA CGAAATCCTG GTCATACATG GTGGGATATC AGAATCCACA
    GACTTGAATA CACTCCACCA GTTACAAAGA AATAAAATGA AATCTGTGTT GATGCCGCCA
    GTGTTGGGAA ATCAAGAGAC TGGCGAAAAG AGAAATAAAT CAGCGTCAAA TTATGTTGAA
    CCAAGGAAGG TTGAACCAGA CAAGACTCCT TCTGAAGACT TGACAAAGCA AGAATGGGAA
    CAAATAGTTG ACATTCTGTG GAGTGACCCC AGAGGCAAAA AAGGCTGTTA TCCAAACACA
    AGCCGAGGAG GGGGCTGCTA TTTTGGACCA GATGTTACCT CTAAGGTTCT TAGTAAAAAC
    CAGTTGAAGA TGCTCATTAG GTCTCATGAG TGTAAACCCG ACGGGTATGA AGTCTCTCAT
    GATGGAAAGG TCATTACTGT ATTTTCTGCT TCTAATTATT ATGAAGAAGG CAGCAACCGA
    GGAGCTTACA TCAGACTAAG TTATGGTACA ATGCCACAAT TTTTCCAGTA CCAAGTAACT
    AGTACATCAT GCCTGAACCC TCTTCACCAA AGGATGAATG CTATGGAATC CAGTGCTTTC
    AAGATACTAA AGGAAAAAAT GATTTCACGA AAAACTGATC TCATCAACGC TTTCGAGCTC
    CGTGACCACA GTAGGTCAGG AAGGATTTCA TTGGCCGAGT GGGCATTTTC TATGGAGAAC
    ATTTTGGGGC TGAACTTACC ATGGAGATCT CTAAGTTCAC ACCTGGTAAC CATTGATTCC
    AGCGGAAGTG TTGACTACAT GTCCAGCTTT GACGATATCC GTATTGAAAA GCCTACGAAA
    GATATGAAGT CCAATCTAAC TGAAACTATG TACAGATACC GATCTGACCT GAAAATCATA
    TTTAATATCA TCGACAGTGA TCAATCAGGT CTGATCTCCA TGGATGAATT TCGGACCATG
    TGGAAACTTT TCAATGCTCA TTACAAAGCT CATATAGATG ATTCCCAAAT TGATGAGCTT
    GCCAGCATAG TGGACTTCAA CAAAGATGGC AACATCGACT TTAATGAGTT TTTAAAGGCT
    TTCTATGTAG TACATAAATA TGATAAGCCA GGGACCAGTC TTGCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu90394
    Clone ID Related Accession (Same CDS sequence) XM_030251335.1
    Accession Version XM_030251335.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1785bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product serine/threonine-protein phosphatase with EF-hands 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGCAGCTAT CCAGCTTCTT TTCGTTCATG TTGGAAAACT ACACAAAGAC AAATAATGAA 
    GATTCAGCAT TAGTAACTCG AATTTTTGAC AACACCAGGC GGGAAAGCCA AATCAAGGAT
    AGAGATGACT TTTTGGGGTT GATAGAAGTG CCTGACTCCT ATGATGGTCC TCGGCTGCAG
    TTTCCTCTGA CGTTTACTGA CATCCATATA CTTCTTCAGG CCTTCAAGCA ACAGCAGATA
    CTTCATGCTC ACTACGTCTT AGAGGTGCTA TTTGAAGCTA GGAAAGTCCT GAAGCAAATG
    CCGAATTTCT CTCATGTAAA AACCTTTCCC GCCAAAGAGA TAACAATATG TGGTGATTTG
    CATGGGAAAC TGGATGATCT TATGCTGATT TTCTATAAGA ATGGCCTGCC TTCAGAGAAC
    AATCCATACG TTTTCAATGG TGACTTTGTA GATCGAGGAA ACAATTCCAT GGAGATCCTA
    ATGATCCTGC TTGTTTGTTT TCTTGTCTAC CCCTCTGACC TGCACCTCAA TAGAGGGAAC
    CATGAAGACT TTATGATGAA CCTGAGGTAT GGCTTCACAA AAGAAATTTT ACAAAAATAC
    AAGCTACACG GGAGAAAAAT CTTACAAGTC TTGGAAGAAG TCTATACCTG GCTCCCAATC
    GGTACAATTA TTGACAACGA AATCCTGGTC ATACATGGTG GGATATCAGA ATCCACAGAC
    TTGAATACAC TCCACCAGTT ACAAAGAAAT AAAATGAAAT CTGTGTTGAT GCCGCCAGTG
    TTGGGAAATC AAGAGACTGG CGAAAAGAGA AATAAATCAG CGTCAAATTA TGTTGAACCA
    AGGAAGGTTG AACCAGACAA GACTCCTTCT GAAGACTTGA CAAAGCAAGA ATGGGAACAA
    ATAGTTGACA TTCTGTGGAG TGACCCCAGA GGCAAAAAAG GCTGTTATCC AAACACAAGC
    CGAGGAGGGG GCTGCTATTT TGGACCAGAT GTTACCTCTA AGGTTCTTAG TAAAAACCAG
    TTGAAGATGC TCATTAGGTC TCATGAGTGT AAACCCGACG GGTATGAAGT CTCTCATGAT
    GGAAAGGTCA TTACTGTATT TTCTGCTTCT AATTATTATG AAGAAGGCAG CAACCGAGGA
    GCTTACATCA GACTAAGTTA TGGTACAATG CCACAATTTT TCCAGTACCA AGTAACTAGT
    ACATCATGCC TGAACCCTCT TCACCAAAGG ATGAATGCTA TGGAATCCAG TGCTTTCAAG
    ATACTAAAGG AAAAAATGAT TTCACGAAAA ACTGATCTCA TCAACGCTTT CGAGCTCCGT
    GACCACAGTA GGTCAGGAAG GATTTCATTG GCCGAGTGGG CATTTTCTAT GGAGAACATT
    TTGGGGCTGA ACTTACCATG GAGATCTCTA AGTTCACACC TGGTAACCAT TGATTCCAGC
    GGAAGTGTTG ACTACATGTC CAGCTTTGAC GATATCCGTA TTGAAAAGCC TACGAAAGAT
    ATGAAGTCCA ATCTAACTGA AACTATGTAC AGATACCGAT CTGACCTGAA AATCATATTT
    AATATCATCG ACAGTGATCA ATCAGGTCTG ATCTCCATGG ATGAATTTCG GACCATGTGG
    AAACTTTTCA ATGCTCATTA CAAAGCTCAT ATAGATGATT CCCAAATTGA TGAGCTTGCC
    AGCATAGTGG ACTTCAACAA AGATGGCAAC ATCGACTTTA ATGAGTTTTT AAAGGCTTTC
    TATGTAGTAC ATAAATATGA TAAGCCAGGG ACCAGTCTTG CTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030107195.1
    CDS384..2168
    Translation

    Target ORF information:

    RefSeq Version XM_030251335.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus protein phosphatase with EF hand calcium-binding domain 1 (Ppef1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030251335.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGCAGCTAT CCAGCTTCTT TTCGTTCATG TTGGAAAACT ACACAAAGAC AAATAATGAA 
    GATTCAGCAT TAGTAACTCG AATTTTTGAC AACACCAGGC GGGAAAGCCA AATCAAGGAT
    AGAGATGACT TTTTGGGGTT GATAGAAGTG CCTGACTCCT ATGATGGTCC TCGGCTGCAG
    TTTCCTCTGA CGTTTACTGA CATCCATATA CTTCTTCAGG CCTTCAAGCA ACAGCAGATA
    CTTCATGCTC ACTACGTCTT AGAGGTGCTA TTTGAAGCTA GGAAAGTCCT GAAGCAAATG
    CCGAATTTCT CTCATGTAAA AACCTTTCCC GCCAAAGAGA TAACAATATG TGGTGATTTG
    CATGGGAAAC TGGATGATCT TATGCTGATT TTCTATAAGA ATGGCCTGCC TTCAGAGAAC
    AATCCATACG TTTTCAATGG TGACTTTGTA GATCGAGGAA ACAATTCCAT GGAGATCCTA
    ATGATCCTGC TTGTTTGTTT TCTTGTCTAC CCCTCTGACC TGCACCTCAA TAGAGGGAAC
    CATGAAGACT TTATGATGAA CCTGAGGTAT GGCTTCACAA AAGAAATTTT ACAAAAATAC
    AAGCTACACG GGAGAAAAAT CTTACAAGTC TTGGAAGAAG TCTATACCTG GCTCCCAATC
    GGTACAATTA TTGACAACGA AATCCTGGTC ATACATGGTG GGATATCAGA ATCCACAGAC
    TTGAATACAC TCCACCAGTT ACAAAGAAAT AAAATGAAAT CTGTGTTGAT GCCGCCAGTG
    TTGGGAAATC AAGAGACTGG CGAAAAGAGA AATAAATCAG CGTCAAATTA TGTTGAACCA
    AGGAAGGTTG AACCAGACAA GACTCCTTCT GAAGACTTGA CAAAGCAAGA ATGGGAACAA
    ATAGTTGACA TTCTGTGGAG TGACCCCAGA GGCAAAAAAG GCTGTTATCC AAACACAAGC
    CGAGGAGGGG GCTGCTATTT TGGACCAGAT GTTACCTCTA AGGTTCTTAG TAAAAACCAG
    TTGAAGATGC TCATTAGGTC TCATGAGTGT AAACCCGACG GGTATGAAGT CTCTCATGAT
    GGAAAGGTCA TTACTGTATT TTCTGCTTCT AATTATTATG AAGAAGGCAG CAACCGAGGA
    GCTTACATCA GACTAAGTTA TGGTACAATG CCACAATTTT TCCAGTACCA AGTAACTAGT
    ACATCATGCC TGAACCCTCT TCACCAAAGG ATGAATGCTA TGGAATCCAG TGCTTTCAAG
    ATACTAAAGG AAAAAATGAT TTCACGAAAA ACTGATCTCA TCAACGCTTT CGAGCTCCGT
    GACCACAGTA GGTCAGGAAG GATTTCATTG GCCGAGTGGG CATTTTCTAT GGAGAACATT
    TTGGGGCTGA ACTTACCATG GAGATCTCTA AGTTCACACC TGGTAACCAT TGATTCCAGC
    GGAAGTGTTG ACTACATGTC CAGCTTTGAC GATATCCGTA TTGAAAAGCC TACGAAAGAT
    ATGAAGTCCA ATCTAACTGA AACTATGTAC AGATACCGAT CTGACCTGAA AATCATATTT
    AATATCATCG ACAGTGATCA ATCAGGTCTG ATCTCCATGG ATGAATTTCG GACCATGTGG
    AAACTTTTCA ATGCTCATTA CAAAGCTCAT ATAGATGATT CCCAAATTGA TGAGCTTGCC
    AGCATAGTGG ACTTCAACAA AGATGGCAAC ATCGACTTTA ATGAGTTTTT AAAGGCTTTC
    TATGTAGTAC ATAAATATGA TAAGCCAGGG ACCAGTCTTG CTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.