Med17 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Med17
Entrez Gene ID	234959
Full Name	mediator complex subunit 17
Synonyms	77kDa,C330002H14Rik,Crsp6,Trap80
General protein information	Preferred Names mediator complex subunit 17 Names mediator of RNA polymerase II transcription subunit 17 CRSP complex subunit 6 cofactor required for Sp1 transcriptional activation subunit 6 cofactor required for Sp1 transcriptional activation, subunit 6 (77kD) thyroid hormone receptor-associated protein complex 80 kDa component
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9\|9 A2

mRNA and Protein(s)

mRNA	Protein	Name
NM_144933.2	NP_659182.1	mediator of RNA polymerase II transcription subunit 17 isoform 1
NM_144933.1	NP_659182.1	mediator of RNA polymerase II transcription subunit 17
NM_001364533.1	NP_001351462.1	mediator of RNA polymerase II transcription subunit 17 isoform 2
XM_006510158.3	XP_006510221.1	mediator of RNA polymerase II transcription subunit 17 isoform X2
XM_006510157.3	XP_006510220.1	mediator of RNA polymerase II transcription subunit 17 isoform X1

Pathways from BioSystems

Homologies

	MED17	XP_001088365.1
Mus musculus (house mouse)	Med17	NP_659182.1
Gallus gallus (chicken)	MED17	NP_001006280.1
Anopheles gambiae (African malaria mosquito)	MED17_ANOGA	XP_319901.2
Xenopus tropicalis (tropical clawed frog)	med17	NP_001016974.1
Pan troglodytes (chimpanzee)	MED17	XP_508698.3
Canis lupus familiaris (dog)	MED17	XP_533975.3
Bos taurus (cattle)	MED17	NP_001029902.2
Homo sapiens (human)	MED17	NP_004259.3
Rattus norvegicus (Norway rat)	Med17	NP_001100271.1
Danio rerio (zebrafish)	med17	NP_001071042.1
Drosophila melanogaster (fruit fly)	MED17	NP_650686.1
Caenorhabditis elegans (roundworm)	mdt-17	NP_001256910.1

Gene Ontology
Bibliography

Related articles in PubMed

Transcriptional activation of lipogenesis by insulin requires phosphorylation of MED17 by CK2.
Viscarra JA, Wang Y, Hong IH, Sul HS
Science signaling10(467)(2017 Feb)

Mediator and cohesin connect gene expression and chromatin architecture.
Kagey MH, Newman JJ, Bilodeau S, Zhan Y, Orlando DA, van Berkum NL, Ebmeier CC, Goossens J, Rahl PB, Levine SS, Taatjes DJ, Dekker J, Young RA
Nature467(7314)430-5(2010 Sep)

ATAC and Mediator coactivators form a stable complex and regulate a set of non-coding RNA genes.
Krebs AR, Demmers J, Karmodiya K, Chang NC, Chang AC, Tora L
EMBO reports11(7)541-7(2010 Jul)

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Yokoyama S, Ito Y, Ueno-Kudoh H, Shimizu H, Uchibe K, Albini S, Mitsuoka K, Miyaki S, Kiso M, Nagai A, Hikata T, Osada T, Fukuda N, Yamashita S, Harada D, Mezzano V, Kasai M, Puri PL, Hayashizaki Y, Okado H, Hashimoto M, Asahara H
Developmental cell17(6)836-48(2009 Dec)

Two modes of transcriptional activation at native promoters by NF-kappaB p65.
van Essen D, Engist B, Natoli G, Saccani S
PLoS biology7(3)e73(2009 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results demonstrate that CK2-mediated phosphorylation of Ser(53) in MED17 is required for the transcriptional activation of lipogenic genes in response to insulin.
Title: Transcriptional activation of lipogenesis by insulin requires phosphorylation of MED17 by CK2.

The following Med17 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Med17 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu11272	NM_144933.2 Latest version!	Mus musculus mediator complex subunit 17 (Med17), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu42113	NM_001364533.1 Latest version!	Mus musculus mediator complex subunit 17 (Med17), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu42113	XM_006510157.3 Latest version!	Mus musculus mediator complex subunit 17 (Med17), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu42114	XM_006510158.3 Latest version!	Mus musculus mediator complex subunit 17 (Med17), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
View Old Accession Versions >>
OMu11272	NM_144933.1	Mus musculus mediator complex subunit 17 (Med17), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Med17 ( NM_144933.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_659182.1 Mus musculus mediator complex subunit 17 (Med17), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu11272
Clone ID Related Accession (Same CDS sequence)	NM_144933.2 , NM_144933.1
Accession Version	NM_144933.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Mus musculus(house mouse)
Product	mediator of RNA polymerase II transcription subunit 17 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ078373.1 and BC020185.1. On Jun 21, 2018 this sequence version replaced NM_144933.1. ##Evidence-Data-START## Transcript exon combination :: BC020185.1, AK033151.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164135, SAMN01164139 [ECO:0000348] ##Evidence-Data-END##

ATGTCGGGCG TGCGAGCCGT GCGCATCAGC ATCGAGTCGG CCTGCGAGAA GCAGGTGCAG 
GAGGTGGGCC TTGATGGCAC CGAGACGTAC CTGCAGCCGC TGTCCATGTC GCAGAACCTG 
GCGCGCCTGG CACAGCGCAT CGACTTCAGC CAGGGCTCTG GCTCTGAGGA GGAGGAGGCG 
GCAGGGCCTG ACGGCGATGC GCCGGACTGG GGGGGCGCGG GAGCCGACCA GGACGACGAG 
GAGGGGTTGG TAAAATTCCA ACCTTCTTTG TGGCCTTGGG ACTCTGTGAG GAACAATTTG 
CGAAGTGCCC TTACAGAGAT GTGTGTTCTC TATGATGTCC TCAGTATTGT CAGGGACAAA 
AAGTTCATGA CTCTGGACCC CGTCTCTCAG GATGCTCTTC CTCCCAAACA GAGTCCACAA 
ACGCTGCAGC TGATCTCCAA GAAGAAGTCC CTCGCTGGAG CAGCTCAGAT CCTACTGAAG 
GGGGCAGAAA GACTGACCAA GTCAGTGGCT GAGAACCAGG AAAACAAGCT CCAGCGAGAC 
TTCAATTCTG AGCTCCTGAG ACTCCGGCAG CACTGGAAGC TGAGGAAGGT GGGCGACAAG 
ATCCTGGGTG ACCTGAGCTA CAGAAGTGCA GGTTCTCTCT TTCCTCATCA TGGTACCTTT 
GAAGTGATAA AGAATACAGA CATTGACTTG GATAAGAAGA TACCAGAAGA TTACTGTCCT 
CTGGATGTCC AAATTCCTAG TGATTTAGAG GGGTCTGCGT ATATCAAGGT TTCTATTCAG 
AAACAGGCTC CAGACATTGG TGATCTTGGC ACAGTTAACC TTTTTAAGAG ACCTTTGCCC 
AAATCCAAGC CAGGTTCCCC ACACTGGCAG ACAAAATTAG AAGCAGCACA AAATGTTCTT 
CTTTGTAAAG AAATTTTTGC ACAGCTCTCC CGGGAAGCCG TTCAGATTAA GTCTCAGATC 
CCTCACATTG TGGTGAAAAA CCAGATCATC TCTCAGCCCT TTCCAAGCTT GCAGTTGTCC 
ATTTCTCTGT GCCACTCCTC AGATGATAAG AAGTCGCAGA AGTGTGCCGC AGAGAAGCCT 
GGGCAAGAGG ATCACCTCTA CGTCCTGGAG CACAACCTGC ACCTGCTGAT CAGAGAGTTT 
CATAAACAGA CCTTGAGTTC CATTGTGATG CCACACCCAG CAAGTGCTCC CTTTGGCCAC 
AAGAGGATGA GACTCTCAGG TCCTCAAGCT TTTGATAAAA ATGAAATTAA TTCAATACAG 
TCCACAGAAG GACTCCTAGA AAAAATAATT AAACAGGCAA AGCATATTTT TCTAAGGAGC 
AGAACTGCTG CAACCATTGA CAGCTTGGCA AGTCGCATTG AGGACCCCCA GATCCAGGCC 
CACTGGTCAA ACATCAATGA TGTGTATGAG TCTAGTGTAA AAGTTTTGAT CACATCTCAG 
GGTTATGAAC AGATCTGCAA GTCCATCCAG CTGCAGTTGA ACATTGGTGT GGAGCAGGTC 
CGTGTTGTGC ACAGAGATGG TCGGGTAATC ATGTTGTCTC ATCAGGAGCA GGAGCTACAG 
GACTTCCTTC TGTCTCAGAT GTCACAGCAC CAGGTGCACG CGGTTCAGCA GCTTGCCAAG 
GTTATGGGCT GGCAGGTCCT CAGCTTCAGT AACCATGTGG GACTTGGGCC CATAGAGAGC 
ATTGGCAATG CTTCGGCCAT CACCGTGGCC TCGCCAAGTG GAGACTATGC GATTTCAGTT 
CGTAACGGAC CTGAGAGTGG CAGCAAGATT ATGGTTCAGT TTCCCCGTAA CCAGTGCAAA 
GACCTTCCGA AGAGTGATGT TTTACAAGAC AGCAAGTGGA GTCATCTCCG AGGACCATTC 
AAGGAGGTTC AGTGGAACAA GATGGAAGGC CGAAACTTCG TTTATAAAAT GGAGCTGCTT 
ATGTCTGCCC TGAGCCCCTG TCTGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_659182.1
CDS	114..2063
Translation	MSGVRAVRISIESACEKQVQEVGLDGTETYLQPLSMSQNLARLAQRIDFSQGSGSEEEEAAGPDGDAPDWGGAGADQDDEEGLVKFQPSLWPWDSVRNNLRSALTEMCVLYDVLSIVRDKKFMTLDPVSQDALPPKQSPQTLQLISKKKSLAGAAQILLKGAERLTKSVAENQENKLQRDFNSELLRLRQHWKLRKVGDKILGDLSYRSAGSLFPHHGTFEVIKNTDIDLDKKIPEDYCPLDVQIPSDLEGSAYIKVSIQKQAPDIGDLGTVNLFKRPLPKSKPGSPHWQTKLEAAQNVLLCKEIFAQLSREAVQIKSQIPHIVVKNQIISQPFPSLQLSISLCHSSDDKKSQKCAAEKPGQEDHLYVLEHNLHLLIREFHKQTLSSIVMPHPASAPFGHKRMRLSGPQAFDKNEINSIQSTEGLLEKIIKQAKHIFLRSRTAATIDSLASRIEDPQIQAHWSNINDVYESSVKVLITSQGYEQICKSIQLQLNIGVEQVRVVHRDGRVIMLSHQEQELQDFLLSQMSQHQVHAVQQLAKVMGWQVLSFSNHVGLGPIESIGNASAITVASPSGDYAISVRNGPESGSKIMVQFPRNQCKDLPKSDVLQDSKWSHLRGPFKEVQWNKMEGRNFVYKMELLMSALSPCLL

Target ORF information:

RefSeq Version	NM_144933.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus mediator complex subunit 17 (Med17), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_144933.2

ORF Insert Sequence:

ATGTCGGGCG TGCGAGCCGT GCGCATCAGC ATCGAGTCGG CCTGCGAGAA GCAGGTGCAG 
GAGGTGGGCC TTGATGGCAC CGAGACGTAC CTGCAGCCGC TGTCCATGTC GCAGAACCTG 
GCGCGCCTGG CACAGCGCAT CGACTTCAGC CAGGGCTCTG GCTCTGAGGA GGAGGAGGCG 
GCAGGGCCTG ACGGCGATGC GCCGGACTGG GGGGGCGCGG GAGCCGACCA GGACGACGAG 
GAGGGGTTGG TAAAATTCCA ACCTTCTTTG TGGCCTTGGG ACTCTGTGAG GAACAATTTG 
CGAAGTGCCC TTACAGAGAT GTGTGTTCTC TATGATGTCC TCAGTATTGT CAGGGACAAA 
AAGTTCATGA CTCTGGACCC CGTCTCTCAG GATGCTCTTC CTCCCAAACA GAGTCCACAA 
ACGCTGCAGC TGATCTCCAA GAAGAAGTCC CTCGCTGGAG CAGCTCAGAT CCTACTGAAG 
GGGGCAGAAA GACTGACCAA GTCAGTGGCT GAGAACCAGG AAAACAAGCT CCAGCGAGAC 
TTCAATTCTG AGCTCCTGAG ACTCCGGCAG CACTGGAAGC TGAGGAAGGT GGGCGACAAG 
ATCCTGGGTG ACCTGAGCTA CAGAAGTGCA GGTTCTCTCT TTCCTCATCA TGGTACCTTT 
GAAGTGATAA AGAATACAGA CATTGACTTG GATAAGAAGA TACCAGAAGA TTACTGTCCT 
CTGGATGTCC AAATTCCTAG TGATTTAGAG GGGTCTGCGT ATATCAAGGT TTCTATTCAG 
AAACAGGCTC CAGACATTGG TGATCTTGGC ACAGTTAACC TTTTTAAGAG ACCTTTGCCC 
AAATCCAAGC CAGGTTCCCC ACACTGGCAG ACAAAATTAG AAGCAGCACA AAATGTTCTT 
CTTTGTAAAG AAATTTTTGC ACAGCTCTCC CGGGAAGCCG TTCAGATTAA GTCTCAGATC 
CCTCACATTG TGGTGAAAAA CCAGATCATC TCTCAGCCCT TTCCAAGCTT GCAGTTGTCC 
ATTTCTCTGT GCCACTCCTC AGATGATAAG AAGTCGCAGA AGTGTGCCGC AGAGAAGCCT 
GGGCAAGAGG ATCACCTCTA CGTCCTGGAG CACAACCTGC ACCTGCTGAT CAGAGAGTTT 
CATAAACAGA CCTTGAGTTC CATTGTGATG CCACACCCAG CAAGTGCTCC CTTTGGCCAC 
AAGAGGATGA GACTCTCAGG TCCTCAAGCT TTTGATAAAA ATGAAATTAA TTCAATACAG 
TCCACAGAAG GACTCCTAGA AAAAATAATT AAACAGGCAA AGCATATTTT TCTAAGGAGC 
AGAACTGCTG CAACCATTGA CAGCTTGGCA AGTCGCATTG AGGACCCCCA GATCCAGGCC 
CACTGGTCAA ACATCAATGA TGTGTATGAG TCTAGTGTAA AAGTTTTGAT CACATCTCAG 
GGTTATGAAC AGATCTGCAA GTCCATCCAG CTGCAGTTGA ACATTGGTGT GGAGCAGGTC 
CGTGTTGTGC ACAGAGATGG TCGGGTAATC ATGTTGTCTC ATCAGGAGCA GGAGCTACAG 
GACTTCCTTC TGTCTCAGAT GTCACAGCAC CAGGTGCACG CGGTTCAGCA GCTTGCCAAG 
GTTATGGGCT GGCAGGTCCT CAGCTTCAGT AACCATGTGG GACTTGGGCC CATAGAGAGC 
ATTGGCAATG CTTCGGCCAT CACCGTGGCC TCGCCAAGTG GAGACTATGC GATTTCAGTT 
CGTAACGGAC CTGAGAGTGG CAGCAAGATT ATGGTTCAGT TTCCCCGTAA CCAGTGCAAA 
GACCTTCCGA AGAGTGATGT TTTACAAGAC AGCAAGTGGA GTCATCTCCG AGGACCATTC 
AAGGAGGTTC AGTGGAACAA GATGGAAGGC CGAAACTTCG TTTATAAAAT GGAGCTGCTT 
ATGTCTGCCC TGAGCCCCTG TCTGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Med17 ( NM_144933.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_659182.1 Mus musculus mediator complex subunit 17 (Med17), mRNA.

Price & Availability↑

CloneID	OMu11272
Clone ID Related Accession (Same CDS sequence)	NM_144933.2 , NM_144933.1
Accession Version	NM_144933.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-29
Organism	Mus musculus(house mouse)
Product	mediator of RNA polymerase II transcription subunit 17
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC020185.1. ##Evidence-Data-START## Transcript exon combination :: BC020185.1, AK033151.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164135, SAMN01164139 [ECO:0000348] ##Evidence-Data-END##

ATGTCGGGCG TGCGAGCCGT GCGCATCAGC ATCGAGTCGG CCTGCGAGAA GCAGGTGCAG 
GAGGTGGGCC TTGATGGCAC CGAGACGTAC CTGCAGCCGC TGTCCATGTC GCAGAACCTG 
GCGCGCCTGG CACAGCGCAT CGACTTCAGC CAGGGCTCTG GCTCTGAGGA GGAGGAGGCG 
GCAGGGCCTG ACGGCGATGC GCCGGACTGG GGGGGCGCGG GAGCCGACCA GGACGACGAG 
GAGGGGTTGG TAAAATTCCA ACCTTCTTTG TGGCCTTGGG ACTCTGTGAG GAACAATTTG 
CGAAGTGCCC TTACAGAGAT GTGTGTTCTC TATGATGTCC TCAGTATTGT CAGGGACAAA 
AAGTTCATGA CTCTGGACCC CGTCTCTCAG GATGCTCTTC CTCCCAAACA GAGTCCACAA 
ACGCTGCAGC TGATCTCCAA GAAGAAGTCC CTCGCTGGAG CAGCTCAGAT CCTACTGAAG 
GGGGCAGAAA GACTGACCAA GTCAGTGGCT GAGAACCAGG AAAACAAGCT CCAGCGAGAC 
TTCAATTCTG AGCTCCTGAG ACTCCGGCAG CACTGGAAGC TGAGGAAGGT GGGCGACAAG 
ATCCTGGGTG ACCTGAGCTA CAGAAGTGCA GGTTCTCTCT TTCCTCATCA TGGTACCTTT 
GAAGTGATAA AGAATACAGA CATTGACTTG GATAAGAAGA TACCAGAAGA TTACTGTCCT 
CTGGATGTCC AAATTCCTAG TGATTTAGAG GGGTCTGCGT ATATCAAGGT TTCTATTCAG 
AAACAGGCTC CAGACATTGG TGATCTTGGC ACAGTTAACC TTTTTAAGAG ACCTTTGCCC 
AAATCCAAGC CAGGTTCCCC ACACTGGCAG ACAAAATTAG AAGCAGCACA AAATGTTCTT 
CTTTGTAAAG AAATTTTTGC ACAGCTCTCC CGGGAAGCCG TTCAGATTAA GTCTCAGATC 
CCTCACATTG TGGTGAAAAA CCAGATCATC TCTCAGCCCT TTCCAAGCTT GCAGTTGTCC 
ATTTCTCTGT GCCACTCCTC AGATGATAAG AAGTCGCAGA AGTGTGCCGC AGAGAAGCCT 
GGGCAAGAGG ATCACCTCTA CGTCCTGGAG CACAACCTGC ACCTGCTGAT CAGAGAGTTT 
CATAAACAGA CCTTGAGTTC CATTGTGATG CCACACCCAG CAAGTGCTCC CTTTGGCCAC 
AAGAGGATGA GACTCTCAGG TCCTCAAGCT TTTGATAAAA ATGAAATTAA TTCAATACAG 
TCCACAGAAG GACTCCTAGA AAAAATAATT AAACAGGCAA AGCATATTTT TCTAAGGAGC 
AGAACTGCTG CAACCATTGA CAGCTTGGCA AGTCGCATTG AGGACCCCCA GATCCAGGCC 
CACTGGTCAA ACATCAATGA TGTGTATGAG TCTAGTGTAA AAGTTTTGAT CACATCTCAG 
GGTTATGAAC AGATCTGCAA GTCCATCCAG CTGCAGTTGA ACATTGGTGT GGAGCAGGTC 
CGTGTTGTGC ACAGAGATGG TCGGGTAATC ATGTTGTCTC ATCAGGAGCA GGAGCTACAG 
GACTTCCTTC TGTCTCAGAT GTCACAGCAC CAGGTGCACG CGGTTCAGCA GCTTGCCAAG 
GTTATGGGCT GGCAGGTCCT CAGCTTCAGT AACCATGTGG GACTTGGGCC CATAGAGAGC 
ATTGGCAATG CTTCGGCCAT CACCGTGGCC TCGCCAAGTG GAGACTATGC GATTTCAGTT 
CGTAACGGAC CTGAGAGTGG CAGCAAGATT ATGGTTCAGT TTCCCCGTAA CCAGTGCAAA 
GACCTTCCGA AGAGTGATGT TTTACAAGAC AGCAAGTGGA GTCATCTCCG AGGACCATTC 
AAGGAGGTTC AGTGGAACAA GATGGAAGGC CGAAACTTCG TTTATAAAAT GGAGCTGCTT 
ATGTCTGCCC TGAGCCCCTG TCTGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_659182.1
CDS	75..2024
Translation	MSGVRAVRISIESACEKQVQEVGLDGTETYLQPLSMSQNLARLAQRIDFSQGSGSEEEEAAGPDGDAPDWGGAGADQDDEEGLVKFQPSLWPWDSVRNNLRSALTEMCVLYDVLSIVRDKKFMTLDPVSQDALPPKQSPQTLQLISKKKSLAGAAQILLKGAERLTKSVAENQENKLQRDFNSELLRLRQHWKLRKVGDKILGDLSYRSAGSLFPHHGTFEVIKNTDIDLDKKIPEDYCPLDVQIPSDLEGSAYIKVSIQKQAPDIGDLGTVNLFKRPLPKSKPGSPHWQTKLEAAQNVLLCKEIFAQLSREAVQIKSQIPHIVVKNQIISQPFPSLQLSISLCHSSDDKKSQKCAAEKPGQEDHLYVLEHNLHLLIREFHKQTLSSIVMPHPASAPFGHKRMRLSGPQAFDKNEINSIQSTEGLLEKIIKQAKHIFLRSRTAATIDSLASRIEDPQIQAHWSNINDVYESSVKVLITSQGYEQICKSIQLQLNIGVEQVRVVHRDGRVIMLSHQEQELQDFLLSQMSQHQVHAVQQLAKVMGWQVLSFSNHVGLGPIESIGNASAITVASPSGDYAISVRNGPESGSKIMVQFPRNQCKDLPKSDVLQDSKWSHLRGPFKEVQWNKMEGRNFVYKMELLMSALSPCLL

Target ORF information:

RefSeq Version	NM_144933.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mediator complex subunit 17 (Med17), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_144933.1

ORF Insert Sequence:

ATGTCGGGCG TGCGAGCCGT GCGCATCAGC ATCGAGTCGG CCTGCGAGAA GCAGGTGCAG 
GAGGTGGGCC TTGATGGCAC CGAGACGTAC CTGCAGCCGC TGTCCATGTC GCAGAACCTG 
GCGCGCCTGG CACAGCGCAT CGACTTCAGC CAGGGCTCTG GCTCTGAGGA GGAGGAGGCG 
GCAGGGCCTG ACGGCGATGC GCCGGACTGG GGGGGCGCGG GAGCCGACCA GGACGACGAG 
GAGGGGTTGG TAAAATTCCA ACCTTCTTTG TGGCCTTGGG ACTCTGTGAG GAACAATTTG 
CGAAGTGCCC TTACAGAGAT GTGTGTTCTC TATGATGTCC TCAGTATTGT CAGGGACAAA 
AAGTTCATGA CTCTGGACCC CGTCTCTCAG GATGCTCTTC CTCCCAAACA GAGTCCACAA 
ACGCTGCAGC TGATCTCCAA GAAGAAGTCC CTCGCTGGAG CAGCTCAGAT CCTACTGAAG 
GGGGCAGAAA GACTGACCAA GTCAGTGGCT GAGAACCAGG AAAACAAGCT CCAGCGAGAC 
TTCAATTCTG AGCTCCTGAG ACTCCGGCAG CACTGGAAGC TGAGGAAGGT GGGCGACAAG 
ATCCTGGGTG ACCTGAGCTA CAGAAGTGCA GGTTCTCTCT TTCCTCATCA TGGTACCTTT 
GAAGTGATAA AGAATACAGA CATTGACTTG GATAAGAAGA TACCAGAAGA TTACTGTCCT 
CTGGATGTCC AAATTCCTAG TGATTTAGAG GGGTCTGCGT ATATCAAGGT TTCTATTCAG 
AAACAGGCTC CAGACATTGG TGATCTTGGC ACAGTTAACC TTTTTAAGAG ACCTTTGCCC 
AAATCCAAGC CAGGTTCCCC ACACTGGCAG ACAAAATTAG AAGCAGCACA AAATGTTCTT 
CTTTGTAAAG AAATTTTTGC ACAGCTCTCC CGGGAAGCCG TTCAGATTAA GTCTCAGATC 
CCTCACATTG TGGTGAAAAA CCAGATCATC TCTCAGCCCT TTCCAAGCTT GCAGTTGTCC 
ATTTCTCTGT GCCACTCCTC AGATGATAAG AAGTCGCAGA AGTGTGCCGC AGAGAAGCCT 
GGGCAAGAGG ATCACCTCTA CGTCCTGGAG CACAACCTGC ACCTGCTGAT CAGAGAGTTT 
CATAAACAGA CCTTGAGTTC CATTGTGATG CCACACCCAG CAAGTGCTCC CTTTGGCCAC 
AAGAGGATGA GACTCTCAGG TCCTCAAGCT TTTGATAAAA ATGAAATTAA TTCAATACAG 
TCCACAGAAG GACTCCTAGA AAAAATAATT AAACAGGCAA AGCATATTTT TCTAAGGAGC 
AGAACTGCTG CAACCATTGA CAGCTTGGCA AGTCGCATTG AGGACCCCCA GATCCAGGCC 
CACTGGTCAA ACATCAATGA TGTGTATGAG TCTAGTGTAA AAGTTTTGAT CACATCTCAG 
GGTTATGAAC AGATCTGCAA GTCCATCCAG CTGCAGTTGA ACATTGGTGT GGAGCAGGTC 
CGTGTTGTGC ACAGAGATGG TCGGGTAATC ATGTTGTCTC ATCAGGAGCA GGAGCTACAG 
GACTTCCTTC TGTCTCAGAT GTCACAGCAC CAGGTGCACG CGGTTCAGCA GCTTGCCAAG 
GTTATGGGCT GGCAGGTCCT CAGCTTCAGT AACCATGTGG GACTTGGGCC CATAGAGAGC 
ATTGGCAATG CTTCGGCCAT CACCGTGGCC TCGCCAAGTG GAGACTATGC GATTTCAGTT 
CGTAACGGAC CTGAGAGTGG CAGCAAGATT ATGGTTCAGT TTCCCCGTAA CCAGTGCAAA 
GACCTTCCGA AGAGTGATGT TTTACAAGAC AGCAAGTGGA GTCATCTCCG AGGACCATTC 
AAGGAGGTTC AGTGGAACAA GATGGAAGGC CGAAACTTCG TTTATAAAAT GGAGCTGCTT 
ATGTCTGCCC TGAGCCCCTG TCTGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Med17 ( NM_001364533.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001351462.1 Mus musculus mediator complex subunit 17 (Med17), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu42113
Clone ID Related Accession (Same CDS sequence)	NM_001364533.1 , XM_006510157.3
Accession Version	NM_001364533.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1812bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-20
Organism	Mus musculus(house mouse)
Product	mediator of RNA polymerase II transcription subunit 17 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC154296.2. On Jun 21, 2018 this sequence version replaced XM_006510157.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660817.1587.1, SRR1660811.31824.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164135, SAMN01164139 [ECO:0000348] ##Evidence-Data-END##

ATGTCGGGCG TGCGAGCCGT GCGCATCAGC ATCGAGTCGG CCTGCGAGAA GCAGGTGCAG 
GAGGTGGGCC TTGATGGCAC CGAGACGTAC CTGCAGCCGC TGTCCATGTC GCAGAACCTG 
GCGCGCCTGG CACAGCGCAT CGACTTCAGC CAGGGCTCTG GCTCTGAGGA GGAGGAGGCG 
GCAGGGCCTG ACGGCGATGC GCCGGACTGG GGGGGCGCGG GAGCCGACCA GGACGACGAG 
GAGGGGTTGG TAAAATTCCA ACCTTCTTTG TGGCCTTGGG ACTCTGTGAG GAACAATTTG 
CGAAGTGCCC TTACAGAGAT GTGTGTTCTC TATGATGTCC TCAGTATTGT CAGGGACAAA 
AAGTTCATGA CTCTGGACCC CGTCTCTCAG GATGCTCTTC CTCCCAAACA GAGTCCACAA 
ACGCTGCAGC TGATCTCCAA GAAGAAGTCC CTCGCTGGAG CAGCTCAGAT CCTACTGAAG 
GGGGCAGAAA GACTGACCAA GTCAGTGGCT GAGAACCAGG AAAACAAGCT CCAGCGAGAC 
TTCAATTCTG AGCTCCTGAG ACTCCGGCAG CACTGGAAGC TGAGGAAGGT GGGCGACAAG 
ATCCTGGGTG ACCTGAGCTA CAGAAGTGCA GGTTCTCTCT TTCCTCATCA TGGTACCTTT 
GAAGTGATAA AGAATACAGA CATTGACTTG GATAAGAAGA TACCAGAAGA TTACTGTCCT 
CTGGATGTCC AAATTCCTAG TGATTTAGAG GGGTCTGCGT ATATCAAGGT TTCTATTCAG 
AAACAGGCTC CAGACATTGG TGATCTTGGC ACAGTTAACC TTTTTAAGAG ACCTTTGCCC 
AAATCCAAGC CAGGTTCCCC ACACTGGCAG ACAAAATTAG AAGCAGCACA AAATGTTCTT 
CTTTGTAAAG AAATTTTTGC ACAGCTCTCC CGGGAAGCCG TTCAGATTAA GTCTCAGATC 
CCTCACATTG TGGTGAAAAA CCAGATCATC TCTCAGCCCT TTCCAAGCTT GCAGTTGTCC 
ATTTCTCTGT GCCACTCCTC AGATGATAAG AAGTCGCAGA AGTGTGCCGC AGAGAAGCCT 
GGGCAAGAGG ATCACCTCTA CGTCCTGGAG CACAACCTGC ACCTGCTGAT CAGAGAGTTT 
CATAAACAGA CCTTGAGTTC CATTGTGATG CCACACCCAG CAAGTGCTCC CTTTGGCCAC 
AAGAGGATGA GACTCTCAGG TCCTCAAGCT TTTGATAAAA ATGAAATTAA TTCAATACAG 
TCCACAGAAG GACTCCTAGA AAAAATAATT AAACAGGCAA AGCATATTTT TCTAAGGAGC 
AGGTCCATCC AGCTGCAGTT GAACATTGGT GTGGAGCAGG TCCGTGTTGT GCACAGAGAT 
GGTCGGGTAA TCATGTTGTC TCATCAGGAG CAGGAGCTAC AGGACTTCCT TCTGTCTCAG 
ATGTCACAGC ACCAGGTGCA CGCGGTTCAG CAGCTTGCCA AGGTTATGGG CTGGCAGGTC 
CTCAGCTTCA GTAACCATGT GGGACTTGGG CCCATAGAGA GCATTGGCAA TGCTTCGGCC 
ATCACCGTGG CCTCGCCAAG TGGAGACTAT GCGATTTCAG TTCGTAACGG ACCTGAGAGT 
GGCAGCAAGA TTATGGTTCA GTTTCCCCGT AACCAGTGCA AAGACCTTCC GAAGAGTGAT 
GTTTTACAAG ACAGCAAGTG GAGTCATCTC CGAGGACCAT TCAAGGAGGT TCAGTGGAAC 
AAGATGGAAG GCCGAAACTT CGTTTATAAA ATGGAGCTGC TTATGTCTGC CCTGAGCCCC 
TGTCTGCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001351462.1
CDS	114..1925
Translation	MSGVRAVRISIESACEKQVQEVGLDGTETYLQPLSMSQNLARLAQRIDFSQGSGSEEEEAAGPDGDAPDWGGAGADQDDEEGLVKFQPSLWPWDSVRNNLRSALTEMCVLYDVLSIVRDKKFMTLDPVSQDALPPKQSPQTLQLISKKKSLAGAAQILLKGAERLTKSVAENQENKLQRDFNSELLRLRQHWKLRKVGDKILGDLSYRSAGSLFPHHGTFEVIKNTDIDLDKKIPEDYCPLDVQIPSDLEGSAYIKVSIQKQAPDIGDLGTVNLFKRPLPKSKPGSPHWQTKLEAAQNVLLCKEIFAQLSREAVQIKSQIPHIVVKNQIISQPFPSLQLSISLCHSSDDKKSQKCAAEKPGQEDHLYVLEHNLHLLIREFHKQTLSSIVMPHPASAPFGHKRMRLSGPQAFDKNEINSIQSTEGLLEKIIKQAKHIFLRSRSIQLQLNIGVEQVRVVHRDGRVIMLSHQEQELQDFLLSQMSQHQVHAVQQLAKVMGWQVLSFSNHVGLGPIESIGNASAITVASPSGDYAISVRNGPESGSKIMVQFPRNQCKDLPKSDVLQDSKWSHLRGPFKEVQWNKMEGRNFVYKMELLMSALSPCLL

Target ORF information:

RefSeq Version	NM_001364533.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mediator complex subunit 17 (Med17), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001364533.1

ORF Insert Sequence:

ATGTCGGGCG TGCGAGCCGT GCGCATCAGC ATCGAGTCGG CCTGCGAGAA GCAGGTGCAG 
GAGGTGGGCC TTGATGGCAC CGAGACGTAC CTGCAGCCGC TGTCCATGTC GCAGAACCTG 
GCGCGCCTGG CACAGCGCAT CGACTTCAGC CAGGGCTCTG GCTCTGAGGA GGAGGAGGCG 
GCAGGGCCTG ACGGCGATGC GCCGGACTGG GGGGGCGCGG GAGCCGACCA GGACGACGAG 
GAGGGGTTGG TAAAATTCCA ACCTTCTTTG TGGCCTTGGG ACTCTGTGAG GAACAATTTG 
CGAAGTGCCC TTACAGAGAT GTGTGTTCTC TATGATGTCC TCAGTATTGT CAGGGACAAA 
AAGTTCATGA CTCTGGACCC CGTCTCTCAG GATGCTCTTC CTCCCAAACA GAGTCCACAA 
ACGCTGCAGC TGATCTCCAA GAAGAAGTCC CTCGCTGGAG CAGCTCAGAT CCTACTGAAG 
GGGGCAGAAA GACTGACCAA GTCAGTGGCT GAGAACCAGG AAAACAAGCT CCAGCGAGAC 
TTCAATTCTG AGCTCCTGAG ACTCCGGCAG CACTGGAAGC TGAGGAAGGT GGGCGACAAG 
ATCCTGGGTG ACCTGAGCTA CAGAAGTGCA GGTTCTCTCT TTCCTCATCA TGGTACCTTT 
GAAGTGATAA AGAATACAGA CATTGACTTG GATAAGAAGA TACCAGAAGA TTACTGTCCT 
CTGGATGTCC AAATTCCTAG TGATTTAGAG GGGTCTGCGT ATATCAAGGT TTCTATTCAG 
AAACAGGCTC CAGACATTGG TGATCTTGGC ACAGTTAACC TTTTTAAGAG ACCTTTGCCC 
AAATCCAAGC CAGGTTCCCC ACACTGGCAG ACAAAATTAG AAGCAGCACA AAATGTTCTT 
CTTTGTAAAG AAATTTTTGC ACAGCTCTCC CGGGAAGCCG TTCAGATTAA GTCTCAGATC 
CCTCACATTG TGGTGAAAAA CCAGATCATC TCTCAGCCCT TTCCAAGCTT GCAGTTGTCC 
ATTTCTCTGT GCCACTCCTC AGATGATAAG AAGTCGCAGA AGTGTGCCGC AGAGAAGCCT 
GGGCAAGAGG ATCACCTCTA CGTCCTGGAG CACAACCTGC ACCTGCTGAT CAGAGAGTTT 
CATAAACAGA CCTTGAGTTC CATTGTGATG CCACACCCAG CAAGTGCTCC CTTTGGCCAC 
AAGAGGATGA GACTCTCAGG TCCTCAAGCT TTTGATAAAA ATGAAATTAA TTCAATACAG 
TCCACAGAAG GACTCCTAGA AAAAATAATT AAACAGGCAA AGCATATTTT TCTAAGGAGC 
AGGTCCATCC AGCTGCAGTT GAACATTGGT GTGGAGCAGG TCCGTGTTGT GCACAGAGAT 
GGTCGGGTAA TCATGTTGTC TCATCAGGAG CAGGAGCTAC AGGACTTCCT TCTGTCTCAG 
ATGTCACAGC ACCAGGTGCA CGCGGTTCAG CAGCTTGCCA AGGTTATGGG CTGGCAGGTC 
CTCAGCTTCA GTAACCATGT GGGACTTGGG CCCATAGAGA GCATTGGCAA TGCTTCGGCC 
ATCACCGTGG CCTCGCCAAG TGGAGACTAT GCGATTTCAG TTCGTAACGG ACCTGAGAGT 
GGCAGCAAGA TTATGGTTCA GTTTCCCCGT AACCAGTGCA AAGACCTTCC GAAGAGTGAT 
GTTTTACAAG ACAGCAAGTG GAGTCATCTC CGAGGACCAT TCAAGGAGGT TCAGTGGAAC 
AAGATGGAAG GCCGAAACTT CGTTTATAAA ATGGAGCTGC TTATGTCTGC CCTGAGCCCC 
TGTCTGCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Med17 ( XM_006510157.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006510220.1 Mus musculus mediator complex subunit 17 (Med17), transcript variant X1, mRNA.

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CloneID	OMu42113
Clone ID Related Accession (Same CDS sequence)	NM_001364533.1 , XM_006510157.3
Accession Version	XM_006510157.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1812bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	mediator of RNA polymerase II transcription subunit 17 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006510157.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGCG TGCGAGCCGT GCGCATCAGC ATCGAGTCGG CCTGCGAGAA GCAGGTGCAG 
GAGGTGGGCC TTGATGGCAC CGAGACGTAC CTGCAGCCGC TGTCCATGTC GCAGAACCTG 
GCGCGCCTGG CACAGCGCAT CGACTTCAGC CAGGGCTCTG GCTCTGAGGA GGAGGAGGCG 
GCAGGGCCTG ACGGCGATGC GCCGGACTGG GGGGGCGCGG GAGCCGACCA GGACGACGAG 
GAGGGGTTGG TAAAATTCCA ACCTTCTTTG TGGCCTTGGG ACTCTGTGAG GAACAATTTG 
CGAAGTGCCC TTACAGAGAT GTGTGTTCTC TATGATGTCC TCAGTATTGT CAGGGACAAA 
AAGTTCATGA CTCTGGACCC CGTCTCTCAG GATGCTCTTC CTCCCAAACA GAGTCCACAA 
ACGCTGCAGC TGATCTCCAA GAAGAAGTCC CTCGCTGGAG CAGCTCAGAT CCTACTGAAG 
GGGGCAGAAA GACTGACCAA GTCAGTGGCT GAGAACCAGG AAAACAAGCT CCAGCGAGAC 
TTCAATTCTG AGCTCCTGAG ACTCCGGCAG CACTGGAAGC TGAGGAAGGT GGGCGACAAG 
ATCCTGGGTG ACCTGAGCTA CAGAAGTGCA GGTTCTCTCT TTCCTCATCA TGGTACCTTT 
GAAGTGATAA AGAATACAGA CATTGACTTG GATAAGAAGA TACCAGAAGA TTACTGTCCT 
CTGGATGTCC AAATTCCTAG TGATTTAGAG GGGTCTGCGT ATATCAAGGT TTCTATTCAG 
AAACAGGCTC CAGACATTGG TGATCTTGGC ACAGTTAACC TTTTTAAGAG ACCTTTGCCC 
AAATCCAAGC CAGGTTCCCC ACACTGGCAG ACAAAATTAG AAGCAGCACA AAATGTTCTT 
CTTTGTAAAG AAATTTTTGC ACAGCTCTCC CGGGAAGCCG TTCAGATTAA GTCTCAGATC 
CCTCACATTG TGGTGAAAAA CCAGATCATC TCTCAGCCCT TTCCAAGCTT GCAGTTGTCC 
ATTTCTCTGT GCCACTCCTC AGATGATAAG AAGTCGCAGA AGTGTGCCGC AGAGAAGCCT 
GGGCAAGAGG ATCACCTCTA CGTCCTGGAG CACAACCTGC ACCTGCTGAT CAGAGAGTTT 
CATAAACAGA CCTTGAGTTC CATTGTGATG CCACACCCAG CAAGTGCTCC CTTTGGCCAC 
AAGAGGATGA GACTCTCAGG TCCTCAAGCT TTTGATAAAA ATGAAATTAA TTCAATACAG 
TCCACAGAAG GACTCCTAGA AAAAATAATT AAACAGGCAA AGCATATTTT TCTAAGGAGC 
AGGTCCATCC AGCTGCAGTT GAACATTGGT GTGGAGCAGG TCCGTGTTGT GCACAGAGAT 
GGTCGGGTAA TCATGTTGTC TCATCAGGAG CAGGAGCTAC AGGACTTCCT TCTGTCTCAG 
ATGTCACAGC ACCAGGTGCA CGCGGTTCAG CAGCTTGCCA AGGTTATGGG CTGGCAGGTC 
CTCAGCTTCA GTAACCATGT GGGACTTGGG CCCATAGAGA GCATTGGCAA TGCTTCGGCC 
ATCACCGTGG CCTCGCCAAG TGGAGACTAT GCGATTTCAG TTCGTAACGG ACCTGAGAGT 
GGCAGCAAGA TTATGGTTCA GTTTCCCCGT AACCAGTGCA AAGACCTTCC GAAGAGTGAT 
GTTTTACAAG ACAGCAAGTG GAGTCATCTC CGAGGACCAT TCAAGGAGGT TCAGTGGAAC 
AAGATGGAAG GCCGAAACTT CGTTTATAAA ATGGAGCTGC TTATGTCTGC CCTGAGCCCC 
TGTCTGCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006510220.1
CDS	1691..3502
Translation	MSGVRAVRISIESACEKQVQEVGLDGTETYLQPLSMSQNLARLAQRIDFSQGSGSEEEEAAGPDGDAPDWGGAGADQDDEEGLVKFQPSLWPWDSVRNNLRSALTEMCVLYDVLSIVRDKKFMTLDPVSQDALPPKQSPQTLQLISKKKSLAGAAQILLKGAERLTKSVAENQENKLQRDFNSELLRLRQHWKLRKVGDKILGDLSYRSAGSLFPHHGTFEVIKNTDIDLDKKIPEDYCPLDVQIPSDLEGSAYIKVSIQKQAPDIGDLGTVNLFKRPLPKSKPGSPHWQTKLEAAQNVLLCKEIFAQLSREAVQIKSQIPHIVVKNQIISQPFPSLQLSISLCHSSDDKKSQKCAAEKPGQEDHLYVLEHNLHLLIREFHKQTLSSIVMPHPASAPFGHKRMRLSGPQAFDKNEINSIQSTEGLLEKIIKQAKHIFLRSRSIQLQLNIGVEQVRVVHRDGRVIMLSHQEQELQDFLLSQMSQHQVHAVQQLAKVMGWQVLSFSNHVGLGPIESIGNASAITVASPSGDYAISVRNGPESGSKIMVQFPRNQCKDLPKSDVLQDSKWSHLRGPFKEVQWNKMEGRNFVYKMELLMSALSPCLL

Target ORF information:

RefSeq Version	XM_006510157.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus mediator complex subunit 17 (Med17), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006510157.3

ORF Insert Sequence:

ATGTCGGGCG TGCGAGCCGT GCGCATCAGC ATCGAGTCGG CCTGCGAGAA GCAGGTGCAG 
GAGGTGGGCC TTGATGGCAC CGAGACGTAC CTGCAGCCGC TGTCCATGTC GCAGAACCTG 
GCGCGCCTGG CACAGCGCAT CGACTTCAGC CAGGGCTCTG GCTCTGAGGA GGAGGAGGCG 
GCAGGGCCTG ACGGCGATGC GCCGGACTGG GGGGGCGCGG GAGCCGACCA GGACGACGAG 
GAGGGGTTGG TAAAATTCCA ACCTTCTTTG TGGCCTTGGG ACTCTGTGAG GAACAATTTG 
CGAAGTGCCC TTACAGAGAT GTGTGTTCTC TATGATGTCC TCAGTATTGT CAGGGACAAA 
AAGTTCATGA CTCTGGACCC CGTCTCTCAG GATGCTCTTC CTCCCAAACA GAGTCCACAA 
ACGCTGCAGC TGATCTCCAA GAAGAAGTCC CTCGCTGGAG CAGCTCAGAT CCTACTGAAG 
GGGGCAGAAA GACTGACCAA GTCAGTGGCT GAGAACCAGG AAAACAAGCT CCAGCGAGAC 
TTCAATTCTG AGCTCCTGAG ACTCCGGCAG CACTGGAAGC TGAGGAAGGT GGGCGACAAG 
ATCCTGGGTG ACCTGAGCTA CAGAAGTGCA GGTTCTCTCT TTCCTCATCA TGGTACCTTT 
GAAGTGATAA AGAATACAGA CATTGACTTG GATAAGAAGA TACCAGAAGA TTACTGTCCT 
CTGGATGTCC AAATTCCTAG TGATTTAGAG GGGTCTGCGT ATATCAAGGT TTCTATTCAG 
AAACAGGCTC CAGACATTGG TGATCTTGGC ACAGTTAACC TTTTTAAGAG ACCTTTGCCC 
AAATCCAAGC CAGGTTCCCC ACACTGGCAG ACAAAATTAG AAGCAGCACA AAATGTTCTT 
CTTTGTAAAG AAATTTTTGC ACAGCTCTCC CGGGAAGCCG TTCAGATTAA GTCTCAGATC 
CCTCACATTG TGGTGAAAAA CCAGATCATC TCTCAGCCCT TTCCAAGCTT GCAGTTGTCC 
ATTTCTCTGT GCCACTCCTC AGATGATAAG AAGTCGCAGA AGTGTGCCGC AGAGAAGCCT 
GGGCAAGAGG ATCACCTCTA CGTCCTGGAG CACAACCTGC ACCTGCTGAT CAGAGAGTTT 
CATAAACAGA CCTTGAGTTC CATTGTGATG CCACACCCAG CAAGTGCTCC CTTTGGCCAC 
AAGAGGATGA GACTCTCAGG TCCTCAAGCT TTTGATAAAA ATGAAATTAA TTCAATACAG 
TCCACAGAAG GACTCCTAGA AAAAATAATT AAACAGGCAA AGCATATTTT TCTAAGGAGC 
AGGTCCATCC AGCTGCAGTT GAACATTGGT GTGGAGCAGG TCCGTGTTGT GCACAGAGAT 
GGTCGGGTAA TCATGTTGTC TCATCAGGAG CAGGAGCTAC AGGACTTCCT TCTGTCTCAG 
ATGTCACAGC ACCAGGTGCA CGCGGTTCAG CAGCTTGCCA AGGTTATGGG CTGGCAGGTC 
CTCAGCTTCA GTAACCATGT GGGACTTGGG CCCATAGAGA GCATTGGCAA TGCTTCGGCC 
ATCACCGTGG CCTCGCCAAG TGGAGACTAT GCGATTTCAG TTCGTAACGG ACCTGAGAGT 
GGCAGCAAGA TTATGGTTCA GTTTCCCCGT AACCAGTGCA AAGACCTTCC GAAGAGTGAT 
GTTTTACAAG ACAGCAAGTG GAGTCATCTC CGAGGACCAT TCAAGGAGGT TCAGTGGAAC 
AAGATGGAAG GCCGAAACTT CGTTTATAAA ATGGAGCTGC TTATGTCTGC CCTGAGCCCC 
TGTCTGCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Med17 ( XM_006510158.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006510221.1 Mus musculus mediator complex subunit 17 (Med17), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu42114
Clone ID Related Accession (Same CDS sequence)	XM_006510158.3
Accession Version	XM_006510158.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1710bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	mediator of RNA polymerase II transcription subunit 17 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006510158.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACCGA GACGTACCTG CAGCCGCTGT CCATGTCGCA GAACCTGGCG CGCCTGGCAC 
AGCGCATCGA CTTCAGCCAG GGCTCTGGCT CTGAGGAGGA GGAGGCGGCA GGGCCTGACG 
GCGATGCGCC GGACTGGGGG GGCGCGGGAG CCGACCAGGA CGACGAGGAG GAGTCCACAA 
ACGCTGCAGC TGATCTCCAA GAAGAAGTCC CTCGCTGGAG CAGCTCAGAT CCTACTGAAG 
GGGGCAGAAA GACTGACCAA GTCAGTGGCT GAGAACCAGG AAAACAAGCT CCAGCGAGAC 
TTCAATTCTG AGCTCCTGAG ACTCCGGCAG CACTGGAAGC TGAGGAAGGT GGGCGACAAG 
ATCCTGGGTG ACCTGAGCTA CAGAAGTGCA GGTTCTCTCT TTCCTCATCA TGGTACCTTT 
GAAGTGATAA AGAATACAGA CATTGACTTG GATAAGAAGA TACCAGAAGA TTACTGTCCT 
CTGGATGTCC AAATTCCTAG TGATTTAGAG GGGTCTGCGT ATATCAAGGT TTCTATTCAG 
AAACAGGCTC CAGACATTGG TGATCTTGGC ACAGTTAACC TTTTTAAGAG ACCTTTGCCC 
AAATCCAAGC CAGGTTCCCC ACACTGGCAG ACAAAATTAG AAGCAGCACA AAATGTTCTT 
CTTTGTAAAG AAATTTTTGC ACAGCTCTCC CGGGAAGCCG TTCAGATTAA GTCTCAGATC 
CCTCACATTG TGGTGAAAAA CCAGATCATC TCTCAGCCCT TTCCAAGCTT GCAGTTGTCC 
ATTTCTCTGT GCCACTCCTC AGATGATAAG AAGTCGCAGA AGTGTGCCGC AGAGAAGCCT 
GGGCAAGAGG ATCACCTCTA CGTCCTGGAG CACAACCTGC ACCTGCTGAT CAGAGAGTTT 
CATAAACAGA CCTTGAGTTC CATTGTGATG CCACACCCAG CAAGTGCTCC CTTTGGCCAC 
AAGAGGATGA GACTCTCAGG TCCTCAAGCT TTTGATAAAA ATGAAATTAA TTCAATACAG 
TCCACAGAAG GACTCCTAGA AAAAATAATT AAACAGGCAA AGCATATTTT TCTAAGGAGC 
AGAACTGCTG CAACCATTGA CAGCTTGGCA AGTCGCATTG AGGACCCCCA GATCCAGGCC 
CACTGGTCAA ACATCAATGA TGTGTATGAG TCTAGTGTAA AAGTTTTGAT CACATCTCAG 
GGTTATGAAC AGATCTGCAA GTCCATCCAG CTGCAGTTGA ACATTGGTGT GGAGCAGGTC 
CGTGTTGTGC ACAGAGATGG TCGGGTAATC ATGTTGTCTC ATCAGGAGCA GGAGCTACAG 
GACTTCCTTC TGTCTCAGAT GTCACAGCAC CAGGTGCACG CGGTTCAGCA GCTTGCCAAG 
GTTATGGGCT GGCAGGTCCT CAGCTTCAGT AACCATGTGG GACTTGGGCC CATAGAGAGC 
ATTGGCAATG CTTCGGCCAT CACCGTGGCC TCGCCAAGTG GAGACTATGC GATTTCAGTT 
CGTAACGGAC CTGAGAGTGG CAGCAAGATT ATGGTTCAGT TTCCCCGTAA CCAGTGCAAA 
GACCTTCCGA AGAGTGATGT TTTACAAGAC AGCAAGTGGA GTCATCTCCG AGGACCATTC 
AAGGAGGTTC AGTGGAACAA GATGGAAGGC CGAAACTTCG TTTATAAAAT GGAGCTGCTT 
ATGTCTGCCC TGAGCCCCTG TCTGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006510221.1
CDS	1764..3473
Translation	MAPRRTCSRCPCRRTWRAWHSASTSARALALRRRRRQGLTAMRRTGGAREPTRTTRRSPQTLQLISKKKSLAGAAQILLKGAERLTKSVAENQENKLQRDFNSELLRLRQHWKLRKVGDKILGDLSYRSAGSLFPHHGTFEVIKNTDIDLDKKIPEDYCPLDVQIPSDLEGSAYIKVSIQKQAPDIGDLGTVNLFKRPLPKSKPGSPHWQTKLEAAQNVLLCKEIFAQLSREAVQIKSQIPHIVVKNQIISQPFPSLQLSISLCHSSDDKKSQKCAAEKPGQEDHLYVLEHNLHLLIREFHKQTLSSIVMPHPASAPFGHKRMRLSGPQAFDKNEINSIQSTEGLLEKIIKQAKHIFLRSRTAATIDSLASRIEDPQIQAHWSNINDVYESSVKVLITSQGYEQICKSIQLQLNIGVEQVRVVHRDGRVIMLSHQEQELQDFLLSQMSQHQVHAVQQLAKVMGWQVLSFSNHVGLGPIESIGNASAITVASPSGDYAISVRNGPESGSKIMVQFPRNQCKDLPKSDVLQDSKWSHLRGPFKEVQWNKMEGRNFVYKMELLMSALSPCLL

Target ORF information:

RefSeq Version	XM_006510158.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus mediator complex subunit 17 (Med17), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006510158.3

ORF Insert Sequence:

ATGGCACCGA GACGTACCTG CAGCCGCTGT CCATGTCGCA GAACCTGGCG CGCCTGGCAC 
AGCGCATCGA CTTCAGCCAG GGCTCTGGCT CTGAGGAGGA GGAGGCGGCA GGGCCTGACG 
GCGATGCGCC GGACTGGGGG GGCGCGGGAG CCGACCAGGA CGACGAGGAG GAGTCCACAA 
ACGCTGCAGC TGATCTCCAA GAAGAAGTCC CTCGCTGGAG CAGCTCAGAT CCTACTGAAG 
GGGGCAGAAA GACTGACCAA GTCAGTGGCT GAGAACCAGG AAAACAAGCT CCAGCGAGAC 
TTCAATTCTG AGCTCCTGAG ACTCCGGCAG CACTGGAAGC TGAGGAAGGT GGGCGACAAG 
ATCCTGGGTG ACCTGAGCTA CAGAAGTGCA GGTTCTCTCT TTCCTCATCA TGGTACCTTT 
GAAGTGATAA AGAATACAGA CATTGACTTG GATAAGAAGA TACCAGAAGA TTACTGTCCT 
CTGGATGTCC AAATTCCTAG TGATTTAGAG GGGTCTGCGT ATATCAAGGT TTCTATTCAG 
AAACAGGCTC CAGACATTGG TGATCTTGGC ACAGTTAACC TTTTTAAGAG ACCTTTGCCC 
AAATCCAAGC CAGGTTCCCC ACACTGGCAG ACAAAATTAG AAGCAGCACA AAATGTTCTT 
CTTTGTAAAG AAATTTTTGC ACAGCTCTCC CGGGAAGCCG TTCAGATTAA GTCTCAGATC 
CCTCACATTG TGGTGAAAAA CCAGATCATC TCTCAGCCCT TTCCAAGCTT GCAGTTGTCC 
ATTTCTCTGT GCCACTCCTC AGATGATAAG AAGTCGCAGA AGTGTGCCGC AGAGAAGCCT 
GGGCAAGAGG ATCACCTCTA CGTCCTGGAG CACAACCTGC ACCTGCTGAT CAGAGAGTTT 
CATAAACAGA CCTTGAGTTC CATTGTGATG CCACACCCAG CAAGTGCTCC CTTTGGCCAC 
AAGAGGATGA GACTCTCAGG TCCTCAAGCT TTTGATAAAA ATGAAATTAA TTCAATACAG 
TCCACAGAAG GACTCCTAGA AAAAATAATT AAACAGGCAA AGCATATTTT TCTAAGGAGC 
AGAACTGCTG CAACCATTGA CAGCTTGGCA AGTCGCATTG AGGACCCCCA GATCCAGGCC 
CACTGGTCAA ACATCAATGA TGTGTATGAG TCTAGTGTAA AAGTTTTGAT CACATCTCAG 
GGTTATGAAC AGATCTGCAA GTCCATCCAG CTGCAGTTGA ACATTGGTGT GGAGCAGGTC 
CGTGTTGTGC ACAGAGATGG TCGGGTAATC ATGTTGTCTC ATCAGGAGCA GGAGCTACAG 
GACTTCCTTC TGTCTCAGAT GTCACAGCAC CAGGTGCACG CGGTTCAGCA GCTTGCCAAG 
GTTATGGGCT GGCAGGTCCT CAGCTTCAGT AACCATGTGG GACTTGGGCC CATAGAGAGC 
ATTGGCAATG CTTCGGCCAT CACCGTGGCC TCGCCAAGTG GAGACTATGC GATTTCAGTT 
CGTAACGGAC CTGAGAGTGG CAGCAAGATT ATGGTTCAGT TTCCCCGTAA CCAGTGCAAA 
GACCTTCCGA AGAGTGATGT TTTACAAGAC AGCAAGTGGA GTCATCTCCG AGGACCATTC 
AAGGAGGTTC AGTGGAACAA GATGGAAGGC CGAAACTTCG TTTATAAAAT GGAGCTGCTT 
ATGTCTGCCC TGAGCCCCTG TCTGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Transcriptional activation of lipogenesis by insulin requires phosphorylation of MED17 by CK2.
Science signaling10(467)(2017 Feb)
Viscarra JA,Wang Y,Hong IH,Sul HS

Mediator and cohesin connect gene expression and chromatin architecture.
Nature467(7314)430-5(2010 Sep)
Kagey MH,Newman JJ,Bilodeau S,Zhan Y,Orlando DA,van Berkum NL,Ebmeier CC,Goossens J,Rahl PB,Levine SS,Taatjes DJ,Dekker J,Young RA

ATAC and Mediator coactivators form a stable complex and regulate a set of non-coding RNA genes.
EMBO reports11(7)541-7(2010 Jul)
Krebs AR,Demmers J,Karmodiya K,Chang NC,Chang AC,Tora L

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Developmental cell17(6)836-48(2009 Dec)
Yokoyama S,Ito Y,Ueno-Kudoh H,Shimizu H,Uchibe K,Albini S,Mitsuoka K,Miyaki S,Kiso M,Nagai A,Hikata T,Osada T,Fukuda N,Yamashita S,Harada D,Mezzano V,Kasai M,Puri PL,Hayashizaki Y,Okado H,Hashimoto M,Asahara H

Two modes of transcriptional activation at native promoters by NF-kappaB p65.
PLoS biology7(3)e73(2009 Mar)
van Essen D,Engist B,Natoli G,Saccani S

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Med17 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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