gRNAs
The following Gucy1a2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gucy1a2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OMu73798 | |
| Clone ID Related Accession (Same CDS sequence) | XM_011242361.2 | |
| Accession Version | XM_011242361.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1941bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-08-07 | |
| Organism | Mus musculus(house mouse) | |
| Product | guanylate cyclase soluble subunit alpha-2 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011242361.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGAACACCA AGTTATTGGG GTTCACAGCT CTTGATGACT TCTCCAAGCA GCCTGGATTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_011240663.1 |
| CDS | 128..2068 |
| Translation |
Target ORF information:
Target ORF information:
|
 XM_011242361.2 |
1 | ATGAACACCA AGTTATTGGG GTTCACAGCT CTTGATGACT TCTCCAAGCA GCCTGGATTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu12595 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001033322.2 | |
| Accession Version | NM_001033322.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2193bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-05-25 | |
| Organism | Mus musculus(house mouse) | |
| Product | guanylate cyclase soluble subunit alpha-2 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC122444.4, AK147527.1, AK165150.1 and AC156642.2. On Jan 27, 2012 this sequence version replaced NM_001033322.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849385 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## | |
1 | ATGTCTCGAA GAAAGATTTC ATCCGAGTCT TTCAGCTCTT TGAGCTCTGA CTACCTGGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001028494.1 |
| CDS | 455..2647 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001033322.2 |
1 | ATGTCTCGAA GAAAGATTTC ATCCGAGTCT TTCAGCTCTT TGAGCTCTGA CTACCTGGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Postsynaptic NO/cGMP increases NMDA receptor currents via hyperpolarization-activated cyclic nucleotide-gated channels in the hippocampus. |
Presynaptic nitric oxide/cGMP facilitates glutamate release via hyperpolarization-activated cyclic nucleotide-gated channels in the hippocampus. |
Phosphodiesterase 5 restricts NOS3/Soluble guanylate cyclase signaling to L-type Ca2+ current in cardiac myocytes. |
More than a retrograde messenger: nitric oxide needs two cGMP pathways to induce hippocampal long-term potentiation. |
The receptor guanylyl cyclase Npr2 is essential for sensory axon bifurcation within the spinal cord. |
